LOCUS       DADVXU010000001        99284 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.001, whole genome shotgun sequence.
ACCESSION   DADVXU010000001 DADVXU010000000
VERSION     DADVXU010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 99284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 99284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..99284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.001"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(551..2164)
                     /locus_tag="KB953_000001"
     CDS             complement(551..2164)
                     /locus_tag="KB953_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBB6865011.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2305..3213)
                     /locus_tag="KB953_000002"
     CDS             complement(2305..3213)
                     /locus_tag="KB953_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6865012.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            3396..4520
                     /locus_tag="KB953_000003"
     CDS             3396..4520
                     /locus_tag="KB953_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBB6865013.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            4545..4724
                     /locus_tag="KB953_000004"
     CDS             4545..4724
                     /locus_tag="KB953_000004"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865014.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            4747..5664
                     /locus_tag="KB953_000005"
     CDS             4747..5664
                     /locus_tag="KB953_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBB6865015.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            5657..6490
                     /locus_tag="KB953_000006"
     CDS             5657..6490
                     /locus_tag="KB953_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBB6865016.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            6573..7691
                     /locus_tag="KB953_000007"
     CDS             6573..7691
                     /locus_tag="KB953_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBB6865017.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            7685..9583
                     /locus_tag="KB953_000008"
     CDS             7685..9583
                     /locus_tag="KB953_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HBB6865018.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            9577..10800
                     /locus_tag="KB953_000009"
     CDS             9577..10800
                     /locus_tag="KB953_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBB6865019.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATMEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(10886..12820)
                     /locus_tag="KB953_000010"
     CDS             complement(10886..12820)
                     /locus_tag="KB953_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBB6865020.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            13046..13702
                     /locus_tag="KB953_000011"
     CDS             13046..13702
                     /locus_tag="KB953_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBB6865021.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            complement(13776..14129)
                     /locus_tag="KB953_000012"
     CDS             complement(13776..14129)
                     /locus_tag="KB953_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HBB6865022.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            14321..15250
                     /locus_tag="KB953_000013"
     CDS             14321..15250
                     /locus_tag="KB953_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBB6865023.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            15261..16097
                     /locus_tag="KB953_000014"
     CDS             15261..16097
                     /locus_tag="KB953_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HBB6865024.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            16345..17121
                     /locus_tag="KB953_000015"
     CDS             16345..17121
                     /locus_tag="KB953_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBB6865025.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            17297..18040
                     /locus_tag="KB953_000016"
     CDS             17297..18040
                     /locus_tag="KB953_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6865026.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            18033..19613
                     /locus_tag="KB953_000017"
     CDS             18033..19613
                     /locus_tag="KB953_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HBB6865027.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(19864..20343)
                     /locus_tag="KB953_000018"
     CDS             complement(19864..20343)
                     /locus_tag="KB953_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBB6865028.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(20359..21111)
                     /locus_tag="KB953_000019"
     CDS             complement(20359..21111)
                     /locus_tag="KB953_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBB6865029.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            21228..21298
                     /locus_tag="KB953_000020"
     tRNA            21228..21298
                     /locus_tag="KB953_000020"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21260..21262,aa:Gly,seq:ccc)
     gene            21659..22384
                     /locus_tag="KB953_000021"
     CDS             21659..22384
                     /locus_tag="KB953_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HBB6865030.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            22639..22905
                     /locus_tag="KB953_000022"
     CDS             22639..22905
                     /locus_tag="KB953_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HBB6865031.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPSYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            22902..23333
                     /gene="ruvX"
                     /locus_tag="KB953_000023"
     CDS             22902..23333
                     /gene="ruvX"
                     /locus_tag="KB953_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBB6865032.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            23358..23663
                     /locus_tag="KB953_000024"
     CDS             23358..23663
                     /locus_tag="KB953_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HBB6865033.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(23744..24889)
                     /locus_tag="KB953_000025"
     CDS             complement(23744..24889)
                     /locus_tag="KB953_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBB6865034.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(24950..25597)
                     /locus_tag="KB953_000026"
     CDS             complement(24950..25597)
                     /locus_tag="KB953_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6865035.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            25791..26084
                     /locus_tag="KB953_000027"
     CDS             25791..26084
                     /locus_tag="KB953_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865036.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            26128..26439
                     /locus_tag="KB953_000028"
     CDS             26128..26439
                     /locus_tag="KB953_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865037.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            26436..27060
                     /locus_tag="KB953_000029"
                     /pseudo
     CDS             26436..27060
                     /locus_tag="KB953_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            27201..27359
                     /locus_tag="KB953_000030"
     CDS             27201..27359
                     /locus_tag="KB953_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865038.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            27360..27722
                     /locus_tag="KB953_000031"
     CDS             27360..27722
                     /locus_tag="KB953_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865039.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            27759..28607
                     /locus_tag="KB953_000032"
     CDS             27759..28607
                     /locus_tag="KB953_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HBB6865040.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            28597..30072
                     /locus_tag="KB953_000033"
     CDS             28597..30072
                     /locus_tag="KB953_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HBB6865041.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            30338..30745
                     /locus_tag="KB953_000034"
     CDS             30338..30745
                     /locus_tag="KB953_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HBB6865042.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            30748..30963
                     /locus_tag="KB953_000035"
     CDS             30748..30963
                     /locus_tag="KB953_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865043.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            30967..31347
                     /locus_tag="KB953_000036"
     CDS             30967..31347
                     /locus_tag="KB953_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBB6865044.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            31481..31636
                     /locus_tag="KB953_000037"
     CDS             31481..31636
                     /locus_tag="KB953_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HBB6865045.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            31705..32178
                     /locus_tag="KB953_000038"
     CDS             31705..32178
                     /locus_tag="KB953_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HBB6865046.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            32175..33869
                     /locus_tag="KB953_000039"
     CDS             32175..33869
                     /locus_tag="KB953_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBB6865047.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            33835..34020
                     /locus_tag="KB953_000040"
     CDS             33835..34020
                     /locus_tag="KB953_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865048.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            34024..35199
                     /locus_tag="KB953_000041"
     CDS             34024..35199
                     /locus_tag="KB953_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBB6865049.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            35192..36715
                     /locus_tag="KB953_000042"
     CDS             35192..36715
                     /locus_tag="KB953_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBB6865050.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            36771..37055
                     /locus_tag="KB953_000043"
     CDS             36771..37055
                     /locus_tag="KB953_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HBB6865051.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            37042..37377
                     /locus_tag="KB953_000044"
     CDS             37042..37377
                     /locus_tag="KB953_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBB6865052.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            37672..38298
                     /locus_tag="KB953_000045"
     CDS             37672..38298
                     /locus_tag="KB953_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865053.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            39040..39876
                     /locus_tag="KB953_000046"
     CDS             39040..39876
                     /locus_tag="KB953_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBB6865054.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLINLYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            39940..40371
                     /locus_tag="KB953_000047"
     CDS             39940..40371
                     /locus_tag="KB953_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB6865055.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(40433..41755)
                     /locus_tag="KB953_000048"
     CDS             complement(40433..41755)
                     /locus_tag="KB953_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HBB6865056.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(41909..43549)
                     /locus_tag="KB953_000049"
     CDS             complement(41909..43549)
                     /locus_tag="KB953_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HBB6865057.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            43882..44352
                     /locus_tag="KB953_000050"
     CDS             43882..44352
                     /locus_tag="KB953_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HBB6865058.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            44574..45677
                     /locus_tag="KB953_000051"
     CDS             44574..45677
                     /locus_tag="KB953_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBB6865059.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLKDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            45961..46641
                     /locus_tag="KB953_000052"
     CDS             45961..46641
                     /locus_tag="KB953_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBB6865060.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            46731..47765
                     /locus_tag="KB953_000053"
     CDS             46731..47765
                     /locus_tag="KB953_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HBB6865061.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            47779..48114
                     /locus_tag="KB953_000054"
     CDS             47779..48114
                     /locus_tag="KB953_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865062.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            48098..50578
                     /locus_tag="KB953_000055"
     CDS             48098..50578
                     /locus_tag="KB953_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865063.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWKLTAGPTS"
     gene            50634..52193
                     /locus_tag="KB953_000056"
     CDS             50634..52193
                     /locus_tag="KB953_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6865064.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            52190..53554
                     /locus_tag="KB953_000057"
     CDS             52190..53554
                     /locus_tag="KB953_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBB6865065.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            53725..54795
                     /locus_tag="KB953_000058"
     CDS             53725..54795
                     /locus_tag="KB953_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6865066.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            54810..55478
                     /locus_tag="KB953_000059"
     CDS             54810..55478
                     /locus_tag="KB953_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6865067.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            55498..56076
                     /locus_tag="KB953_000060"
     CDS             55498..56076
                     /locus_tag="KB953_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBB6865068.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            56477..57388
                     /locus_tag="KB953_000061"
     CDS             56477..57388
                     /locus_tag="KB953_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBB6865069.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(57476..58393)
                     /locus_tag="KB953_000062"
     CDS             complement(57476..58393)
                     /locus_tag="KB953_000062"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HBB6865070.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            58595..59032
                     /gene="zapA"
                     /locus_tag="KB953_000063"
     CDS             58595..59032
                     /gene="zapA"
                     /locus_tag="KB953_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBB6865071.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            59091..59639
                     /locus_tag="KB953_000064"
     CDS             59091..59639
                     /locus_tag="KB953_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HBB6865072.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            59754..62114
                     /locus_tag="KB953_000065"
     CDS             59754..62114
                     /locus_tag="KB953_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBB6865073.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEEQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLYDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            62201..62515
                     /gene="trxA"
                     /locus_tag="KB953_000066"
     CDS             62201..62515
                     /gene="trxA"
                     /locus_tag="KB953_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBB6865074.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            62697..64475
                     /gene="uvrC"
                     /locus_tag="KB953_000067"
     CDS             62697..64475
                     /gene="uvrC"
                     /locus_tag="KB953_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBB6865075.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            64620..65519
                     /locus_tag="KB953_000068"
     CDS             64620..65519
                     /locus_tag="KB953_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6865076.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            65512..66774
                     /locus_tag="KB953_000069"
     CDS             65512..66774
                     /locus_tag="KB953_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6865077.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            66935..68833
                     /locus_tag="KB953_000070"
     CDS             66935..68833
                     /locus_tag="KB953_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HBB6865078.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(69163..69882)
                     /locus_tag="KB953_000071"
     CDS             complement(69163..69882)
                     /locus_tag="KB953_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBB6865079.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            70219..70551
                     /locus_tag="KB953_000072"
     CDS             70219..70551
                     /locus_tag="KB953_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865080.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            70538..72541
                     /locus_tag="KB953_000073"
     CDS             70538..72541
                     /locus_tag="KB953_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HBB6865081.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            72560..73030
                     /locus_tag="KB953_000074"
     CDS             72560..73030
                     /locus_tag="KB953_000074"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HBB6865082.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            73051..73635
                     /locus_tag="KB953_000075"
     CDS             73051..73635
                     /locus_tag="KB953_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HBB6865083.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            73650..74636
                     /locus_tag="KB953_000076"
     CDS             73650..74636
                     /locus_tag="KB953_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HBB6865084.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            74626..74937
                     /locus_tag="KB953_000077"
     CDS             74626..74937
                     /locus_tag="KB953_000077"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HBB6865085.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            74959..76740
                     /locus_tag="KB953_000078"
     CDS             74959..76740
                     /locus_tag="KB953_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HBB6865086.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            76733..78109
                     /locus_tag="KB953_000079"
     CDS             76733..78109
                     /locus_tag="KB953_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HBB6865087.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            78116..78751
                     /locus_tag="KB953_000080"
     CDS             78116..78751
                     /locus_tag="KB953_000080"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HBB6865088.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            78781..80136
                     /locus_tag="KB953_000081"
     CDS             78781..80136
                     /locus_tag="KB953_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HBB6865089.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(80328..81722)
                     /locus_tag="KB953_000082"
     CDS             complement(80328..81722)
                     /locus_tag="KB953_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBB6865090.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRTQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            81814..82785
                     /locus_tag="KB953_000083"
     CDS             81814..82785
                     /locus_tag="KB953_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HBB6865091.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            82782..83564
                     /locus_tag="KB953_000084"
     CDS             82782..83564
                     /locus_tag="KB953_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBB6865092.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(83733..84104)
                     /locus_tag="KB953_000085"
     CDS             complement(83733..84104)
                     /locus_tag="KB953_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBB6865093.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            84238..84681
                     /locus_tag="KB953_000086"
     CDS             84238..84681
                     /locus_tag="KB953_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HBB6865094.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(84724..85533)
                     /gene="yidA"
                     /locus_tag="KB953_000087"
     CDS             complement(84724..85533)
                     /gene="yidA"
                     /locus_tag="KB953_000087"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBB6865095.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNNEHGVAEAIKKYVL"
     gene            complement(85552..86919)
                     /locus_tag="KB953_000088"
     CDS             complement(85552..86919)
                     /locus_tag="KB953_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBB6865096.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(86903..87733)
                     /locus_tag="KB953_000089"
     CDS             complement(86903..87733)
                     /locus_tag="KB953_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HBB6865097.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            87896..88333
                     /locus_tag="KB953_000090"
     CDS             87896..88333
                     /locus_tag="KB953_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HBB6865098.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            88409..89029
                     /locus_tag="KB953_000091"
     CDS             88409..89029
                     /locus_tag="KB953_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HBB6865099.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            89218..89724
                     /locus_tag="KB953_000092"
     CDS             89218..89724
                     /locus_tag="KB953_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HBB6865100.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            89741..90640
                     /locus_tag="KB953_000093"
     CDS             89741..90640
                     /locus_tag="KB953_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBB6865101.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            90727..91611
                     /locus_tag="KB953_000094"
     CDS             90727..91611
                     /locus_tag="KB953_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBB6865102.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(91803..91874)
                     /locus_tag="KB953_000095"
     tRNA            complement(91803..91874)
                     /locus_tag="KB953_000095"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(91839..91841),aa:Arg,seq:ccg)
     gene            91934..93268
                     /gene="rpoN"
                     /locus_tag="KB953_000096"
     CDS             91934..93268
                     /gene="rpoN"
                     /locus_tag="KB953_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBB6865103.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            93417..94508
                     /locus_tag="KB953_000097"
     CDS             93417..94508
                     /locus_tag="KB953_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HBB6865104.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            94549..95550
                     /gene="gap"
                     /locus_tag="KB953_000098"
     CDS             94549..95550
                     /gene="gap"
                     /locus_tag="KB953_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBB6865105.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            95660..96850
                     /locus_tag="KB953_000099"
     CDS             95660..96850
                     /locus_tag="KB953_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBB6865106.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            96954..97709
                     /locus_tag="KB953_000100"
     CDS             96954..97709
                     /locus_tag="KB953_000100"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBB6865107.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            97825..99123
                     /gene="eno"
                     /locus_tag="KB953_000101"
     CDS             97825..99123
                     /gene="eno"
                     /locus_tag="KB953_000101"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBB6865108.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT        32917 a        16943 c        20475 g        28949 t
ORIGIN      
        1 taaataaata ggaacaaaaa aattaaatta tttaatacac acattaggga tcattcattg
       61 aaggattaga agacaacgag gatggcgaaa aatatgatta caatagaaaa gtcgttctaa
      121 accaatcagt tcctagtatc acacaacgca agccagtcga tttggtgaga ttagaggagt
      181 gaaagcaaac tttattatat aagaaaaaat attcgaaagg gatgctagtt taggtactag
      241 tgctgataga ttaatatgat gcctcgtaat attaggcgta gttactctag agacatcact
      301 atggactatg atttgtctca gagtcacaac atgttttttt atgaaaagaa acagctaaaa
      361 taattaaatt gcgcaatgtt taaactttct taaactataa cctaataaat gtgaagaaat
      421 aatgcacaat ttcttcacat ttatcttcta ttctatatac ataccaacca acaaccctat
      481 atactttttc atttttactc ctcaagtaaa aataactcct agaccgcggc tacccaccgc
      541 ggtcgttttt ttattcttca atcaaaatcg ctttgatacc caaacctgcc aagattcggg
      601 cagaagtata agtattcaat cctcttctga aagtaatgta aactttttta tcttttggga
      661 tttcattgat tcgatctcgt aactcattca ttggaatatt ttttgttgcc gagatactgc
      721 cagaaggtgc tttattaggt tcacgtatat ctaaaaagaa cgcttctttc aaatcgtttt
      781 cgggtatatc acttgctttg atcgtttcta cgatattcgt catttgattg attgcgacat
      841 aaccagctat attcaacggg tcacgggtag tagaatacgg tggagagtaa ggcaactcca
      901 aatcaggcaa atcaaagact gtcaagtttc cggtgattgc tacagaaagt tctcccatcc
      961 gtttatcgac acctttttct ccaaatgctt gtccacctaa aatacgaccg ctatctgcat
     1021 caaaaatcag ctttagattt aatcttgtag cttctggata gaaataggca tggtcaaagg
     1081 gagtgataaa aacagtttta taatttgtta tgcctgatgc ttttaattca tgttctgtca
     1141 ttcccgcata acttgctgta tgatcaaaga tttttgcgac acctgcacca atatatccgc
     1201 gatagcgcat aggcgtacca tttaaaatat ctgccagcat atgtccttga cgattggccg
     1261 cactggaaag catactttgg atcggctgac cagtcactac actagtcgtt tcgataatat
     1321 cgccaatcgc atagatgtcc ggcagattgg tttgtaactg gtcatcaaca atgatctgac
     1381 ctgtatctga taattgtatg ccggttgctt tcaccacttc attcttcgga gaaacaccaa
     1441 cagcaaaaat tagcatatca gcagaaagga cggagccatc tgataatact atttctcgac
     1501 ctttatcacg gatctcagta actcttgttt ctaaatgaac ggctaatcct tctttcagca
     1561 atttgtcata aactaaatta gcaatttctg gatcataagg gaaagcgact tgtggcaatt
     1621 gatcgattaa agtcacgtct aagccgcgat gcttgaaatt ctcagctaat tccagaccca
     1681 taacacctgc accaagaatg acgacatgtt gtggattttt ttcttcaagg aacgatttga
     1741 ttcgatccgc atccgtcaca ctacggagga caaagccgtt ttctgcttca aaaactccgg
     1801 gaatatccgg ataatctggt ctagcaccag aagagatgat caacttatcg taatctgttt
     1861 ttatttcttc atttgtggat aggtctttaa ctgttaatcg cttagtagaa ggatcgattg
     1921 ctgttacttc gtgtttagta aagacatcga tgttgttttt tgttttcaat atttctggtg
     1981 tacgttcgat cagcgagtca cggtcagtga tcacacctcc taaataataa ggcaaagcac
     2041 aacttgcata agaaatattt tctccgcgtt catagataat gatttcgtgc gcttcattca
     2101 atcgacggaa acgagtggca aatgaaggac cgccagctac accgccaatg acaacaactt
     2161 tcataaaaac atctcctttt ctacgttgat cgtactatgt tatggtaaaa aacagcaaat
     2221 aattgtgccg gaaaattatt gtttattttg aatagacaga aaatatagag aaataatttg
     2281 tggataaaac ctaaaaattt ttttctattt taaggataga taatactttg aaggggataa
     2341 ccctgtatat tttttaaaag ctgaagaaaa atgatggatc gaatgaaatc ctaatatatc
     2401 tgcaatttct gtataggtta attcattttc tagcacgaga cgttttgctt tttccatttt
     2461 tagttcttta aaatagctaa taacactttt tccattcact tttctaaaat gtttttttaa
     2521 gctggctgga ctgataaaaa aaatttgggc taattgttca agcaaaaaat tttctgaaag
     2581 atgttcttct aaatatcgta tgattggatc aactaattca tctgaataaa gaatatgctt
     2641 ttttttagga atgactttct catttgaagg tattgtcaat aaagcaagtt cctgttgata
     2701 atgccttggt aaggaagaca attgaatttt ttgcagacaa taagtaacta ataaggaaag
     2761 caaatgaatg ctcatctgtt ggcatagtat gaaaaaagaa ttgatttcct gatcatagga
     2821 aatagctggc ttactatgtt ttagataatc aattttgaac tgttcatttt tcagaaaagt
     2881 aatgatagat gttatttctt gatctataga aaaagattgt cccgtaatat gtgctaataa
     2941 ttgctcatca aaataaaact ggataatgat aaaagtttgt gtttcctttt gagtagaaaa
     3001 gatttggact tcttgctgtt tatcaatgaa taaaagggaa ggattatcta gttctataga
     3061 tttatgtgca aattcagcat ataaattagc gctttcattt attagtagtc gaaaataggg
     3121 agcagcagat aaatgaagat gggtatctat ggaataggtt agtgtttttt tgacaagatt
     3181 ggaggattgg ttgtttgtta acaaaagaac cactttcctt tctcttttta tataaaaagc
     3241 tttctattta taattaatga ctattataac aaaaatgaaa agccaaaacg acaagtaaaa
     3301 tattttttta tataaatatt tgtgttacta attccgttaa tatacataag tgaaagcgtt
     3361 ttatataaga acttgaaaag aaagaaggga tagacatgaa aaaaagatac aaattggtga
     3421 tagctttgct aaacattacc ctattattca gcttaagtgc atgtggaaaa actaacgaag
     3481 aagtaacagc aaacatcgca gatccaatga taaaaaaagc cactacatta gaagtatatg
     3541 tcgcaaacgg caaactgaca aactatgttg aaaatgctgc aaaaatctat aaccgcaaaa
     3601 ctggagcaaa tattgactta aaaattgtga atgtagcttc gggaacagca acagtacaaa
     3661 tgatcacccc aaaattggta acacacgaag atatgccgga tattatctcc atggcagaca
     3721 cttcagcagc aggtgtgctg gaaaaatttc aagatgcttt ttatagtgct gatgaatatg
     3781 gattttatga caaatatggc gatcagtttt atgatcaaaa attgaatatt ttgcgtcaac
     3841 aaacgagtaa tgaaacgatt attccttggg ctagtgattt taccccagca ctttgttatt
     3901 atcaaccaaa aaaatttgct gaaataggtg tagattttga agaaatcagt tcttgggaag
     3961 aatatatcca ggtggcaaaa gcattaaaag aaaaaactgg tagttttgga attgccttgc
     4021 cagaatctgg ggaccaagaa ttgtttatca acatgctagc ccagcagaat caatctttgc
     4081 ttgatgaaga aggaaatatc cgtctgaata ctaaagaagc gaaacatgct gcaaagttaa
     4141 tcaaagaaat gatcgactca gatattgtac atttctacgg tgcacaagat gctgagaaag
     4201 catttcaaga atctgcaatg tttgttgctg gcggctggta tgctacgaat atgtctttga
     4261 attttcctga tgcatctgac aaatggcgca tggctccact gattcctttt tcaaaagaaa
     4321 atccaggaaa aggtgcagta tcaggaggat caagttttta tgtccctaaa gatgcaaaaa
     4381 atccacaagt ggcacaacaa tttcttacat ttatgctgac agatgaagaa tgcttagcca
     4441 acgcactgga gctgggcgtg gctacatcta actcattggc ctatcaaaca gaagcagctg
     4501 agaaaaagtt tgactactaa ggaggacaaa aatactatga actattgaat aaaatcaatg
     4561 aaaatacatc aaatactgtt ttaccagcca cttattcaga tgctacgtct tatttgacta
     4621 ccgctgcata taactattgg caaacaggtg attttgaaaa ttcatatcta aaagaagcag
     4681 aaaattttgc tcataagtac ggcgtaaaat taactggtga ttaaaaaata ttaggaggac
     4741 ttaacgatga tgcaatcttc gttaccaagc aagacaataa agaacagcaa gaaaaaagca
     4801 acagatacgt ctcataaaaa aagtgcttat ggattggtca caccttttgt tattttcttt
     4861 attgtgacga ttttgattcc catcattatc tcgtttgctt ttagttttat gaaaattggc
     4921 tactcatttg aatttgttgg atttgacaat tatctagcta tcttccgaga tccattgttt
     4981 attaaatctt acacgaatgt tttgattatc atggcaggat caattccaat aacgatgcta
     5041 ttagctattt actttgctgt tttattgaat tcagcacagt taaaaggaaa aggatttttt
     5101 agaactgtct attatattcc aaccattaca tcaacggtgg ctgtagcttc cgtattcatg
     5161 acttttttca atccgactgg gttattcaat ggatttttga atgcgctagg aattaacggt
     5221 gtacagtggt taacaaatcc tttttggata cgaatatcaa tgatcatcac gatgatttgg
     5281 cttagtgtag gatataacac tattttattt ttagctggtc ttcaagggat ttctaaagag
     5341 atatacgaaa gtgctgaaat agatggagca tcgaaatttc ggcaattctt tttattaacg
     5401 atccccatgc taaagccaat cattttaatg gccacagttt tagccactat caatggcttg
     5461 ggtaatttca atatcccaaa tatattcttt gggacttcca atggaccaga aaattcagct
     5521 ttggtcgtag gagtcaattt atataaaaca agttttgaaa tggttgattt tggaaaagcc
     5581 tctgctattg cctggacgat ggtgtttgta tctgttatat tatcggctat tcaatttaaa
     5641 ttcggaggtg ataaaaatga ctaaacaaaa gatacaaagc agaattttta cgtacgtcat
     5701 tattgtgttt ggtgccttaa tatttttgat gccatattta tatatgattg tagcttcaac
     5761 gcaaagtaat caagaaattt taagtggcgg aatcaatttc aagtttgggg atatgctatt
     5821 aaaaaactgg caagccatca tggcgagata taattatccc ttgattattt ggaattcgat
     5881 tgttattgct gtaatcagta cagttttagc tacagccagt tctacaatgg cgggttatgc
     5941 attagcaaag tatcgtttta tggggaataa actgttgttc aatacagtta tgctatctag
     6001 gatggtgcct ttttttacca cattgattcc tttattttac attatgagtc gtttaggatt
     6061 atcgaatact tatgcaggtg tgatcatccc ttcgattgcc agtacaactt cagtgtttat
     6121 gatgcgacaa tatagcagtc aatttcctac agcactgatg gaggctgcca gaatcgatgg
     6181 tgccaatgag tgggtgattt ttacgaaaat cgctgtaccg attttatcac ctacgattat
     6241 tactagtggt ttacttatct ttatgggaag ttggaatcaa tatctatttc cattagtcat
     6301 gctttcagat aaagataaat atacggttcc cttaattatc aaaaatatga cggtttctag
     6361 tacaggtgag ccaattaatt atggcgcaat tatgctaatt ttagcaactt cagtgatccc
     6421 aatggtcttg atttatgtgt gggcgcagtc taaatttaag gagaatgaca ttggagctgc
     6481 aaataagtaa aaaaaataac gaatgttaac catctagttc gttctaggag caactccaca
     6541 ttcataaaaa ttaaaagaat ggcgggatat ccatgaatga acagacgaag gaatggtcaa
     6601 gaaaaatttt gaatgtagct tctgaaaaga tagatgcgga attagacaga atggggaata
     6661 aaattccata tatttcttac aacggaaaat atacagacgt aataaaggac agaggaatcg
     6721 actggtggac caatggtttt tggagtggta ttttatggca gctctttcac aagacaaaac
     6781 gagaaaagtt caaagaggca gcaatacaac aagaacaatt attagaatct gccctgtacc
     6841 atttttcaga cgttcatcat gatgtaggtt ttttattctt accatcggct attgcacatt
     6901 atcaacagtc tcaaaacaaa catagtcgac aactagggtt acatgcagct acggtacttg
     6961 ctggacgcta taatatttca gggaactatt tagtagcatg gaatgaccat aaacctggtt
     7021 ggatgattat tgatagtatg atgaatttac cattactgta ttgggcttcg agggaaactg
     7081 gtgatccgag gttctctaaa attgctcggc agcatgcaga tacaacagct aagtatctag
     7141 tgaggaaaga tggatcaacc gggcatatcg cagaatttga tgcagaatca gggaaatata
     7201 ttcggttact tggcgggcaa ggatacaatg agaattcttg ttggagcaga ggaaatgcat
     7261 gggctttata tggattcgcg ttaagttatc gttacacaaa aaatccgtac tatctagcta
     7321 tagcagaaaa gatagcagta aattttattc agaaaatcga aaaaactggt tatattcctc
     7381 tcattgattt tgctgtaaaa gattcgaata ttgataccag tgctggagta tgcgctgcat
     7441 gcggtcttct ggaattagca ggatatgttt cctctgtacg atcaaaaatg tatcatgaag
     7501 ctgctatcaa tatactccaa aagacagaaa agcactatgc aaattggaat atggatgaag
     7561 atgggatcat tggaggcgga gcagaagcat accatagaga actgacttat aatgtgccac
     7621 tgatctattc tgattatttt tttattgaag gcttacttcg attagaacag tcggaattag
     7681 ctatttggta gtaaaaagag gagaaaatac aatgaaacat ccagtaccac tattaaggac
     7741 cagacaggat tactttgact tatttaaaaa gataaatcaa ccactccagc cgttttatcg
     7801 agcaagtggc gcacaaattg attatataca tcaaggggta ggatatggaa ataaaattgc
     7861 aggaatggaa ggattctctc gggttttatg gggagcgggg cccgcaattg accaattaga
     7921 tgaacgatgg ttgaaattaa ttctatcagg aataaaagct ggaacagatc cttcccatcc
     7981 cgattattgg ggagaaatac atgaaagaga ccagcgtatg gtagaaatgc ccgcaatgtt
     8041 attagcgtta tatcatcatg aacaattatt atggaaaaaa ttagatacag gagaaaaaag
     8101 ccagattatt cattggttct cgcaaatttt tgaatatgaa tgtgcagatg gtaattggca
     8161 attttttaag ataattgtcg gaacaattat ccaaaaattt ggttatccag ttaaagaatc
     8221 agcactgaaa gaagcatttg ataatataga aaattgttat cttcaaaatg gctggtatcg
     8281 agacagttca agaggtcgcc aagattatta taatcctttt gcatttcatt attatggttt
     8341 gatttatagc gtactagaac cagaaaagcc cattagccag atatataaga accgagctat
     8401 tgatttttct agagactata ttcatttttt tgcggaagat ggtgcaaatg tgccttttgg
     8461 tcgaagcatg atttatcgtt ttgcagtttc aagcttttgg tcagctatgt tgtgggccga
     8521 tcttttgcct tttaaattag gtgaaatcaa ggggttagtc aatcggaata taagatggtg
     8581 gatgcaaaaa gaaatatttg atgaaaaagg tatcctgaat attggttata cctaccctca
     8641 attgacattg actgaaccct ataatagtac gttatctcca ttatggctga ataagatttt
     8701 tttattgttg gcgctacctg aagaccatga gtattggacg gctaaagaaa tgcctatgcc
     8761 tattcgtgaa ggaactaaac ttctttcaga agccaatttg ctcatacaac atgataatgg
     8821 acatacattt tttttgaatg cgggacaatt agggccaaat ttccatacat tgaccaatga
     8881 aaaatattta aaatttgctt attctagtca atttggtttt tcgataccgc gagcgaatca
     8941 attgaaagca gaaatggcaa tggattcaat gctagggatt cagcgagcgg acacatatat
     9001 cacgaccagt tttaagaatg agagcatcaa aacctatgga cagttcatcg ttcgaaataa
     9061 tgtacgagac attgtatgta agaaagaggt acttgcctct acatgggaac ctactttaca
     9121 ggcaaaaatc agaacttggc ttattccttt tgaaggctgg cagattcgcg tacacaaagt
     9181 agaattagat acagaatatg ttctatatga gacagggttt gctattgaat gttctcctga
     9241 gaaagaagga ataatcatag aagaagacaa ggaaaactat agggtgagtg aagctggctt
     9301 ttcaggtatt atctgtttaa gtgacgatac tattaaacga aaatccacag ctattatggg
     9361 tttgcccaat acaaacttga tgacatggga aaacactttt attcctggat tagagggtac
     9421 atttggaaaa ggaacccatt ggctcggaac aggagtggtt gctcatcaag atcgggatta
     9481 tagtttagaa gtatggaaca atcgtcccaa aattgatttt gatgggacaa ccgggctaac
     9541 tatccaaaat aaaaacaata aaaaaagact aaaattatgc taaaggctgg tggggaaatg
     9601 cttgataaag tgatgtttga agagataata catccagaaa aatggataaa tgaatgttat
     9661 aaagaattaa tggaaaaaga atggaataat gagatcgagc gttgtcgcag acacacggag
     9721 aggttaatca aacgtatcag taacagtcag tatcaattcc caagcgagtg tcctataaaa
     9781 ggacgttatc cattaagaga caatattggt tggacagaag gattttggac aggaattaat
     9841 tggttgttat ttgagaagac gggtgatcct ttatttcaac aaacagctga aaaagcagat
     9901 atagatttta tccacaggat tgactgtcaa attatcacta atcatcacga tcttggtttt
     9961 ttgtattctt tatcaaccgt agcagattat aaaataacgg gaaatcgaaa aatgaaggaa
    10021 gcttcaattg atgctgcaga aacattactt cagcgttttt gggagaaggc aggagttata
    10081 caagcttggg gagatcctag tgatccttca caacaaggta gaatgatcat ggattgttgc
    10141 atgaatcttc cattattgta ttgggcgagt gaagaaactg gaaatcaaaa atataaaaaa
    10201 gctgctcagg ctcatattca aaatgcagca gattatttag taagagaaga tggttcaact
    10261 tatcatacat tttatatgga tgtcaaagat ggaaaaccaa gatatggaag tacgcatcaa
    10321 ggttatagtg ataattcctg ttgggctaga ggccaagcat gggggattta cggttttgca
    10381 ttaagttatc gttatttgaa agaacaaaaa tattttgatt taagcaaaat attagccaat
    10441 tattttatga atcggttgcc agaagatacc atttgttatt gggatttgat ctttgatgat
    10501 tctagcagag aagaaaaaga tacatctagt atggtgatca tggtttgtgg attattagaa
    10561 ttaatagaat caggtctatt agacaaaaca gaaaagaact tataccagtc tgctgtttgg
    10621 aaaattatgc gacaattgaa agaaagatat gctacaatgg aagaagaaga tggcttttta
    10681 acacagtctg tttataataa gcccaaaaat aatggggtaa atacaccttg tatttggggt
    10741 gattactatt atttagaagc tttagtgaga atgactagta attggtcttc ttattggtga
    10801 aaaaggtaaa tatggaattt ttgtttgata aaataaaaaa agaaaaacca aaacagcatg
    10861 aatggtttct gttttggttt tttcgttaat tttcatattc ttctaattcc aagcttagtt
    10921 cttcccattc cgtcaatttt gtttcttgat cttgtctagc agcttctaaa tcttggttca
    10981 gctcattgag ttttacgtga tcttccaggt tttctggcgc gaccatttct tgttccaact
    11041 gatcgatcca ttcatcgatc cgcgccattt cttcttcaac ctgagtaatt tttcgttgaa
    11101 ggctacggat aagcttttgc tgttctttgc tttggtaata atcagatttg ttcgtgggag
    11161 caggagcaga ttctggctca tcttgtgcca acaattcggc gatttcttct tcttcctgct
    11221 ttttctctaa ataataatca tagtctccta gatagacttt gctgccattt tcagagagtt
    11281 cgacgacttt tgtcgcgatc cgattgatga aatatcggtc atgggaaaca aaaagcagtg
    11341 tgccgtcata atcgatcaat gcattttcta atacttcttt gttgtcaata tctaaatggt
    11401 ttgttggttc atccaaaatc aaaaaatttt ctttgttcat cgctaacttg gctaaagcaa
    11461 cccgtgcttt ttcaccgcca cttagcagag ggattggttt ttctacatct tcgccagaaa
    11521 ataagaaagc acctaaagca cttcggatct ctttttctgg agtggtagga tgctcatccc
    11581 acaattctgc taggatcgtc ttgtttgtat gcagatcagc ttgaccttga tcgtaatagc
    11641 cgatggtgac atttgtccct agcgtttctt ttccacggat aaaagggatc tggtgaatga
    11701 ttgatttcag caacgtcgat ttcccgatac cattcggacc tactagagca atcgcttctt
    11761 gacgacggat atctagattg attggttcag ataaggtgac attatcatat ccgatagcgg
    11821 catcttcgat ttgcaagact acatttccag aaggcttttc tatactgaat agaaagtttg
    11881 cttgtttttc atccccttga ggacgatcca gccggtccat tttttctaat gtctttctgc
    11941 ggctttgcgc tcgttttgtg gtagaggcgc gaacaaggtt ccgagcaacg aagtcttcta
    12001 atttatggat ttctgtttgc tgcttttcat aagccttcca ttcacttgct aactgttctg
    12061 ctttcaaatc tagatatttc gaatagttac ctttgtaatg gcgcattttt cttctgctta
    12121 attcatagac ttcattgact actttatcta aaaaataacg gtcatgggaa acaatcaata
    12181 atgcaccagt atagctttgc agataattct caagccagga aagtgtctcg atatctaaat
    12241 ggttcgttgg ttcatctaga atcaaaatgt cagggtgttg caaaagcatg cgagctaaag
    12301 ctaaacgtgt gcgctgtccg cctgataaag tatcgatttt ctgatcataa aatgaagaat
    12361 cgaatttaaa cccatgcaat acggaacgaa tttcattttc ataaccatag ccgtttcgat
    12421 ctgaaaaatc gtgttgcagc cggtcgtatt cttttaaata agaactataa gcaggtgtgt
    12481 ctggcgtacc ttcgctaatg aaaatctcta gctcgcgcat ccgctcttcc atttgtctaa
    12541 cttgttcaaa agctttcagc atctcgttcc aaactgtttc attggaatca agtcctgtgt
    12601 cctgagcgag gtatccgaga gtcgctgttt tatttttagc gatctttcct tcatcaggtg
    12661 cttcaattcc agcaatgatc tttaataaag tcgattttcc agcgccattt ctgccaacta
    12721 aagcgacacg agcgcctgta ctgatttcta aatgtatgtt ttcaaataag acttctgcac
    12781 caaaatggcg agcaatctga ttggcttgta ataatatcat ggaatcctct tttctacttt
    12841 gtgaattctc ctcaaataaa aaactatttt tcaattagta gtctaccata aattccatta
    12901 tactgcgaga tttgcagaaa tgataaataa atgaataaag aataaaattt cacaatatct
    12961 gagcaatgga attgattttt tcctataaat actgctatga tggagaaatc aaagatgtaa
    13021 ttagacaata cgtggaggtc atttcgtgaa agatcatatt attccaaagg cgacagctaa
    13081 aagactgcct ttgtattatc gctatttaag gattttaaat aatgctggaa agaaaaaagt
    13141 gtcttcaact gaattaagtg aagccgttca agtagatagt gcaacgatcc gtcgagattt
    13201 ttcttatttt ggcgaattag gaaaacgtgg gtatggttat gatgtagaag atcttatgca
    13261 cttttttgca aagacattaa acgacgatca attgacaaat gttgcgctga tcggtgtagg
    13321 gaacttggga agtgctttac taaaatacaa attccatcaa agtaatagca tacgtatcag
    13381 ttgtgctttt gatgtgaatg aggaaatcgt tgggcgcatc gtagacggta tccccgttta
    13441 tccaatggaa gatatgtcag aacaaatcaa gatccaacaa atcgaagtag caattttaac
    13501 gatacccgct gaaagagcac aagaagtagt caatcagtta acggaagcag gaatcaaagg
    13561 aatcttgaac tttaccgctg ctcgattgaa tgctccgtca gatgtattga tccaaaatgt
    13621 tgatttgacg aatgaattgc aaaccttgat ctatttcttg cattctgacg caaatgcgca
    13681 ttccgaaaat acagcagaat aaaaataaaa agcgattgaa caaatgaagg caaaactttc
    13741 acttgttcaa tcgctttttt ctgtttggac aacgattatt ttgttcggaa gatttcttca
    13801 taagaaaggg aatcatcttc tgctgtcagt tcatctagta gataacggat ttttttaaag
    13861 tcgatgaacc gtgcttgccg aaagacttct tttccttgat gaaaaatcag gacaactggc
    13921 gcagtcagca cttcaaattt actagctact tctggtgttt caaatgcatc caaatgaagt
    13981 gccggaatat cataatgtgt gagaagttcc tctagtcgtg gacgaacagc gtgacaaaca
    14041 ctgcaattag gcatcgaaag ataaatgacg gcaacggatg actgttgcag tatgtcttga
    14101 acttcagtca agttagtggt ttgtttcatt tttattctcc cttttatgat agaagtgata
    14161 tgtatgagac cttaatttaa tgggaataac agaaaaagtc aattgaagtg ctttggtaaa
    14221 aaataagagt tgtgtaaaaa tttacagagc aacttgtttt ttttgaataa aagcgtatat
    14281 ttgcaaagcg aatgaactag aaataaaaag gaggaaactc atgttttctt ttattccaaa
    14341 ttcggttttg cagatgagta cgattttctt atcgatcgtt attgaagcat tacctttcgt
    14401 attattaggc tgtcttatct caggcgcctt acaagtattt ttgaccccag aacgtgtgaa
    14461 gagatggctt cctgaaaatc gtatcttctc tatttttact ggttcgatct taggattttt
    14521 ctttccttca tgtgaatgcg gcatcgtacc gatcgttcat caatttgtta aaaaaggcgt
    14581 accagttcac acagcgtttg cttttatgct gactgctccg atcatcaatc caatcgtgat
    14641 tttttccact tttatcgcat ttggcaattc ttggcagatg gcaagctggc gggtgatagg
    14701 cagctttttt gttgcccttg tgattggtgt ctggcttgcc tattttcaaa aagacgctgt
    14761 attgaaagaa aaaatcacgt ctaaagtagc agaaaatcac catcatcatg tccatgaaga
    14821 agaacaaagt gagaagaatg aaccaaaaaa tcagctgatc catcaaactg ggcatgtgtt
    14881 gacacattca atcgacgaat tttttgatac aggtcgttat ttgatcattg gcggattaat
    14941 cgcagctgcg atgcagactt atttaccaac tagaattatg ctaacgctcg gctcaacaaa
    15001 gattttagcc attatcatca tgctgttatt ggcctttacg atgtctcttt gctcagaagc
    15061 agatgcgttt ataggatctt ctttgttaag tctgttcggc acagcacctg tagctgcctt
    15121 tttggtcttt ggcccaatgg tcgatatcaa aaatctactg atgatgaaac gatattttac
    15181 aggacgcttt attttatcgc tgatcggact gattgccctt agcgttattg gattcacatt
    15241 atttatttag gagggaaaat atgctgcggt ttttgatttt atctgggtat ttcgaattga
    15301 tgatgtactt gcaagtttct ggcaaactcg atcaatatat caatgtccat tatcgctact
    15361 tagctattct ttcaatgatt ttatctgctc ttttggcttt agttcagctg tatttgtggg
    15421 tcaaaaacgg aaaagcagaa gaaaaggcac acgagcacga tcatgaccat gggttgtcaa
    15481 aaccttatca acgtggaatc gcttatgtac tgctagcttt gccagtaatc gtggggacgt
    15541 tgtttccaac agttagtctc gacactacga tcgtagaagc caaaggattt aatttcccag
    15601 taagtaaaga atcagtaggg gatccggaga tgcagacaca atacttaaag ccggatacca
    15661 gtatttattt caacaaatca gactacttag accaaatgaa tgagttgaaa gaaaagtatg
    15721 gggataagca aactatcaag atcacagatg aaaactattt agaagttatg gagctaattt
    15781 ataattaccc tagcgaattc attggcaaaa aaatcagcta tgaaggattt gtttaccaaa
    15841 caccgaacga tagcaataca gatatcttct tgttccgatt cggcattatc cattgtgtgg
    15901 cagattcagg agtctttggt ttgttgacgc atatgccgga aggagtaacg gttaaaaatg
    15961 atgaatggta taaaatagaa ggaaccatcc aatcagacta ttatcaacca ttcaagcgtg
    16021 aaatcccatc tgtagttgtt acaaacgctg agaaagtctc tgctccgaaa aatcagtatg
    16081 tgtatcgttc tttttaaaag aacaaaataa ataaacatag ctcagacagc tgacacgcaa
    16141 ggacgtcggt tgtctggctt tttttgcatg atgtattcat ttgtttgata tttattttag
    16201 aagcagcatc ctgaaagtat ttgatatcgg tttcaaaggt gttggaaaag attgtgaaga
    16261 gaattacaat ttattcctaa catgtatgtt gaaaagttgt atgaaatgat tttgatagta
    16321 aaatatcgag tggaggtact ttttatggta acgcgtatat ttattttgga tgatagtttg
    16381 gtttttgaaa aaatgctagt agaaattttg gaagaatcca gaattttatt aagtccttgg
    16441 aaatttgaga tcgttcaggg attgaacgtt atacaatttg ttgaattcat taaaaataat
    16501 gaaatctatt cctcagatat ttttttccta gacatcgatt tgaacgccta ttatagcggt
    16561 atcgaactag gcgaaatgct tagagaaaag aatgaaaagt gtaaaattgt ttatctaacg
    16621 tctcatccga ataaagcaat aactgttatt aatcgaaaga ttacgcctag tggttatttg
    16681 ataaaagaat tcacttatga aaaaaataaa aaaagcatcc aaagttactt attgaaagaa
    16741 aaagaaaact atcctgttca acatgctacg aaaaaaaggt ggacaactgt ccgttatggt
    16801 aatagcgatc actatatcca ttacgatgaa atcctttatc taatgagcat gccaggttat
    16861 aaaaataaaa taggcctagt attgaaagat gaagagattc tcgtaaacgg cacaattaaa
    16921 aaatataaaa aatcactcga atatgagtat ttatgtaaag atttaaaagc ttatatcatt
    16981 aatactgaaa acattacatc tattagcaga gtaaacgaaa tagtaacatt tgcaaatgga
    17041 gaacaactag agatgggaaa gagaagtatt gataaactaa agaattttat gcaagaaaag
    17101 atagaaaatg aatcatttta aagaaaacaa agactcatat cttacctgtt agaagagtgt
    17161 tttcctgtag aagagaacaa cccaagtagc agcctagtcg tttctatcct tttttgttat
    17221 gcactctttt tgacatttac tccaccaata acgaagggta caataaaaag taataacaaa
    17281 caaaaggagt acagaaatga ttgaattcat caatgtaagt aaaagttacg ggaaaaaaca
    17341 agcattaaaa gacttgtctc tctcgataag acaaggggaa atttttggat ttttaggaca
    17401 taatggtgcc ggaaaatcaa ctacgattaa aagtttagtt agtattattg aaccatcatc
    17461 gggaacgatt ttagtagatg gaatgaaatt gacggaaaat cgattgagta tcaaacaaaa
    17521 aatcggttat gtgcctgatt ccccagatat ttttttacaa ttgacggctg gagaatattg
    17581 ggacttgatt tcagctgcct atgaattaga cacacaaaaa aaagaaaagc gtttagcaga
    17641 attgacagcg ctttttgata tgtatagtca tcaaaatgaa acaatcgcaa gtttttcaca
    17701 tggaatgcgt caaaaaacga ttttgatcgg cacgttgctg ccagatccag atatttgggt
    17761 attggatgaa cctttgcaag gacttgatcc tcaagcagca tttgatttga aagaaatgat
    17821 gaaggctcat gccgcaaaag gaaaaacagt cattttttct acacatgtat tggataccgc
    17881 acaacagtta tgcgataaac tggctatttt gaagaaaggt gaattgattt atcagggggc
    17941 agttagtgaa ttattgaatg gctcacctga tgaaacgcta gaaaagattt atttgaaaat
    18001 ggctggaagg caagcgacag gagatggacc gtatgtataa gcgacaatta ttagaattaa
    18061 ttcgagttaa tcttttatat gttaatccac agctcacgaa taaagcaaga gagaaaggaa
    18121 aaaagggaaa gcgattaact atctatcttt tgtctcaaaa tcttttttca gggctgattt
    18181 ttttactgat ttacggattt gctatgtttg ctatgaactt tgttaaattg ccaggtttct
    18241 ttacttatta cgtggcactt tttggtatgt taggtttaac tcaaagtata tcagtcattt
    18301 ataatatctt ttttgaagga aatgacctgc aagtattttt gcctctgcct tttggacaag
    18361 aacaggtttt ttcagcaaag attttggtag ttgctttgac catcattcct tttgtttttc
    18421 cgcttctagt catgtttatt ttaaccggat ggcgagctga tctagggatt ttgcttacta
    18481 tcattatctc aatccttttg ttttctctat ttttgattat tcttttttgt ctggctagtt
    18541 tgattgtttt tggtttggct cgaacagcag tgtttaaaaa acatagaaaa acagtttcta
    18601 gtatcttgct tggcagctcg atgctgatcg catttgctgg aatcatgtgg atgaatcatc
    18661 aaacggggac tattgaaaat gagttaacag atcggcatcc gatttcgata ttattgccat
    18721 tctatgatgc tgccagtcag ccactttctc aacaaggttt attgggttgg ggaatactag
    18781 ctatcacggc tctgggattt ttccttgctg taaaacagct gatagtacca aagcttacag
    18841 agcaactgac tgattcaaac agtgagttca aaataaaaag aacccataaa aagaaccaaa
    18901 atgtgcatca gcttttattt agttacaatg ctcaattagt aaaagaacct aatttgatta
    18961 tgcaagttct ttcttcttcc ttgttaactc caatcatttt tattcttgct tttgccctag
    19021 gaggcgaaat aaaattaact gatttagata ataagttaat cggtgttgtt ttcctggtag
    19081 ggatcgcatt ggctttctta acagttaatc agacttcttt tatcagcaat cttatttctt
    19141 tggatcaaga aaattttctg tttatccgtt ctttgccaat ctctatgaac caatatttaa
    19201 aagaaaagtt cagattcgga tggatattgc agagtttttt aactggtggg atcgcattat
    19261 taagcggact gtcttttcag ctgcctttgt tttttatccc tagcttgcta tttggttctt
    19321 tattcggctg ctacttattg tcactacgtt atttcgctag agattatcgg ttgctgctgc
    19381 tgaactggac caatattaac caattattta acagaggttc aggaagtttg ggtttagtcg
    19441 caactatgat gggttcattg atcatcagta ttattcttat cgtcttatac ggaatgttgg
    19501 ctttgtttta tccattttgg ttagtgaata ttcctgtgat cggtttagtt ctaatacttt
    19561 ccgctctatg gatcgtttat tatcaacgaa cgttttggaa aaaatttgaa taaagaagag
    19621 gggccgtgac aaaaatcatg tcgcagcccc ttttttacag atagagggtg ttcaaaagtc
    19681 agtttctcag tattaagtca attttatgta aaaatgtgag aagctatttt tacaaaactt
    19741 tttttaatac tcataattac ttcttattgc tcctgcggtt acttgtcgca atgtttgtga
    19801 gatattttgt catcgctcaa ggctgaacgt ctctttcaca accttttctt tgttacactt
    19861 cgtttattta aaaagccgtt tttttcgttt catcatgatt gcgtcacgct ttactttgaa
    19921 attacggatg ttatggttaa agacttctga taatacaaga cctaaagcga ttgctccagc
    19981 aatcataatt gcttggaccg cataatgagc cgcttctcga taggattcag taacgagatt
    20041 tcgaaccgct tgataagcta aaccaccagg gactagagga accatcccgg gaatattgaa
    20101 aatcgttacg ggcattttca aacgtttaga gaaaagatcg ctgatgaatg ctactcctaa
    20161 agaaccaagc aatgaaccaa aagcagcaga tccgcctatt tgaacacaga tccagtagat
    20221 catccagcca aaagcaccag aaagaccaca acaaatcaat gaacgcctag gtacatttgt
    20281 aataacggca aatgccgcag tcgctaaaaa actaaaagaa aactgaataa atatcgtccc
    20341 catacttgtg ttcctttcct aataaaaaag ctggaaaata aaagcaatcg caaaaccgat
    20401 catcgtagca gtcatcatcg cttctgttcc gcgagaaact cctgagacat aatgtccagc
    20461 tagcaaatca cggatagcgt tcgtaatttg aactccagga actaaaggca tcacacatcc
    20521 gataatgatt agatcctgat tgcttcctaa gccaaaacgt acgctgtaga tagcggctat
    20581 gccaatcaat aaagaagata aaaattccga taaaaatttg attctaaaaa atttcaaact
    20641 atatagataa gctgcgtatc ccaatcctcc aatgactaaa gtcacaggta aatcggtcat
    20701 ctttccgcca aataacacca taattgttcc actgatcact gccgcgctaa cgatttgcaa
    20761 ccagacagga aagaaagaac ggtctcggtc gattgcttgc agttcgcagt aaagttcttc
    20821 taatgttagc tctgaagcaa catatttacg tgaaagatca tttactttta ctactttttc
    20881 taaattaatc gaacggttgg taatttgttc cattcggatg gtcgaggtac gatcaagacc
    20941 aataaagaga ccggtttgag tgacatagct gaccaaacgg aaatttccag aagctaaagc
    21001 aattcgactc atcgtatctt ctacccgata catctccgca ttgctttcca tcatgatttt
    21061 cccagcgagt aaacaagtgt ccagcaacag atctaattct tttcccgtca tagcatcttc
    21121 tccaataatt gattgattag ttttatttta ctacaaaaca taccagaagc gtaaagaata
    21181 aaaaaacttg aggaaggaat aaaagaggcg tatactgttt ttgtattgcg ggtgtagttt
    21241 agtggtaaaa tcccagcttc ccaagctgat gtcgcgggtt cgattcccgt cacccgctta
    21301 aaataggttg tgaaaaagct gctctgcaac aagtaataag aacagccaag caaaaacaac
    21361 ttcttatgtt tttaacttgg atggacttat taccgcagat gcaagctttt gcgcaccgtt
    21421 taccaagcgg ttgtgaaatc agtgtattgt cttgagtatg aagaaaattt ttgaataata
    21481 ataaatacct tacagataga gtggtgactg attagagacg aggaatccgt ctcttttttg
    21541 tatttttaag ccatcttaaa acaggtttta ttttttgctg tttctatttc tttctagtat
    21601 ttatttatag gggaacatat gttaagattt agggagctgt tagtaaagga gtaaagaaat
    21661 gagcaaaaaa acaaccaagg cgatcgtggg gatcgtcgct gctgtgattg caggggcatt
    21721 cggtgtttcc accacacaaa aagattcaga tatctttcag cagattacaa ctatttttga
    21781 ttccacacca caagcttctc aagtagcgga tgtgccagaa tatgatggaa aacatcaaga
    21841 aatagaaatc aatgggaatc agccggaatt tacaaaagag gatcttagcc tttccaaagg
    21901 aacatgggaa agctattcaa atattgataa attgaacaga gtaggacaag ccaatgctat
    21961 gcttggaaaa gatttgttcc ctactgaaaa gagagagcct ttgtatattg acccgacagg
    22021 ttggaaacaa aagaaattaa gtgacgggca atggttatat aatcgcagcc atttaattgg
    22081 tttccagctg actggtcaaa ataacaacat taaaaacttg atgacaggga cacgttcgtt
    22141 gaatgctcca tacatgcttg ctcacgaaaa tgatattgcg gcatatatca aagaaacaaa
    22201 tcatcatgta cgctaccgtg tcactcctca ttttgagggg aatgaactgg tagccagggg
    22261 agtgcaattg gaagcagaaa gcattgaaga caaaaaaatt gaattcaatg tctttatcta
    22321 caatgttcaa gatggctaca cgatcaatta tgagacagga caagcaacaa aaacaaaaaa
    22381 ttagtagcag ctattcgaat tttttatgaa tattatgaaa aaattgtgta aaaacataca
    22441 ttcaaagaaa tatagcgtat actttatgaa aagagtactt ttgataaagc tacagaaaaa
    22501 aggaaaaaaa catacaaata gtgtttctta atttattcgg aagtttggaa tatttgtttg
    22561 tagttttccc tttcttcgag taaaataagg ttgtagaagg taagctggaa ttaccttgcg
    22621 ggaaagaggt gtagaatcat gggttttaca gatgaaaccg tacgttttgg ttttgacgat
    22681 agtcgcaaaa aagaagtgag cgaaacatta gcgattgttt atcgggcttt ggaagaaaaa
    22741 ggctacaatc caatcaatca gattgtcggc tacttgcttt ctggagaccc gtcctacatt
    22801 cctcgttatc gtgatgctcg aaatttgatt cgccgtcacg aacgcgatga gatcatggaa
    22861 gtaattgtca aggactacct ctcaaatcat ggagtgaatt tatgagagta atgggcttgg
    22921 atgtcggttc taagacagta ggtgttgcta tcagtgaccc ctttggctgg acagctcaag
    22981 gagtagagat cattcggatc aatgaagcag aagaagagtt tggctttgat cggttgaagg
    23041 aattagtagc acaatatgaa gtagaccggt ttgtagttgg attacctaaa aatatgaaca
    23101 attccatcgg accaagggca gaagcttcca tgtcatacgg agaaaagatc aaagaactat
    23161 tctccttgcc agttgattat caagatgaac gattaaccac tgttcaggca gaacgtatgc
    23221 tagtagagca ggcaaatact tcaagagcca aacgaaaaaa agtcatcgat aaattagcag
    23281 ctgtgatgat cttacaaaat tatttagacg gctcaggtaa gcaattattt taataataaa
    23341 agaaaagagg taatcttatg acagaacata accattccca cgaccatgaa ggacacgaac
    23401 acatcacatt ggtggatgac caaggaaatg aaacattata tgaaatccta ctaacagttg
    23461 acggacaaga agaatttgga cgtaactatg ttcttttata tcctgctggt gtttcagaag
    23521 atgaagatgt agaattacaa gcttatgcat acatcgaaaa cgaagatgga acagaaggcg
    23581 agttagaaca aatcgaaaca gacgcagaat gggacatgat tgaagaagta ttcaatacat
    23641 tcatggcgga agaagaagaa tagactataa agctaggaga aaccttgata tgatagggtt
    23701 tctccttttt ttatttttta ctgtatgcca tattgtatgc catctagtca atatttaagt
    23761 gttgtactaa tttttcaaca gtcttttctt tcgtttcttt tgccaagtga ccgtaacaat
    23821 cgatcgtctg actaattttt gtatgccgta atctagtttg aacttctacc atagtagctc
    23881 cagcgtccaa gagcattgaa gcatgagtgt gtcttgctac atgcacaccg agaaatggaa
    23941 ccatagcttt ttcacaaata gtaatcaatc ttgcataaat agcgtggtgt gtgattagtt
    24001 ctttatagat accacaaaaa atcatagtcg gatcagttac accaagtgtc agcatatatt
    24061 tccgctgatt aatctgccat ttttttaata tagaaattgt tctttcatct agtggaagaa
    24121 ccccagcact acaatcagtt ttaggatcac tgattttata gccatttttg gattggctta
    24181 gtgttttatt gatggtgacc gtattttctt gaaaatcaat gtctgaccaa ttcaatgcaa
    24241 gggcttcact gatcctaacc ccgctataaa agagaaagcg tagtaacgtt ttatcaaagt
    24301 cgtttcgata agtttcaggt ttgctgttta ggtaatcaaa caaggtgatc agttgttctt
    24361 tactgtaaac tttgaccttt tctttatgct tagtggtggt tcttttaggc attaatacgt
    24421 tgtctagtgg attagtctca ataatcccaa gattgatagc gtagctaaaa atacgcttag
    24481 aagttgaatg aagtaccttg tattggtcag tagtttcagc ccagctattt acaatttgtt
    24541 gggctttttt taccgtgact tcattcacac gaagatttcc atattttgga agaatccact
    24601 ttctaaactt gatttcagtt ttcatgaagg tggcttctcg gacagtggtt ttatatgttt
    24661 ctagccacaa gtcataaact tctttaaacg tttcgttgtt cgctttttta ataccgtttt
    24721 tatcgtagtc cacttgcaag cgtgacaagg caagctgggc ttcttttttt gttttgtaac
    24781 ctcgtctacg aaccttatca ggtttgccag tcattttgtt cattccaaga taggcttcat
    24841 agagccaaaa tttacctttt ttagtttcat attgcttgaa tgttgccata atagttttcc
    24901 ctccatagtg tagcaagccg tgggggcggt tggaggaggc agtttattat tattttgaat
    24961 agtgtttatt tagctcagta acaagagctg tgaactcttt tagaagatca ttttcataaa
    25021 attcttgata ctcttttttc tcatctgtat ttttttctga atcattaatt aatgagtcat
    25081 atcgtaaaag cgtatttaga agagctgcga gataagcaat tggggtactc ccgtcaggaa
    25141 attcaattcg agatcctgtt ttaagatatt ttattgcgtt tgttaaataa atcttctgtg
    25201 agacagtcag gttatcttct tcgatgttat tgatagcatt agaaagatta gaatcaatta
    25261 tagagttatt tttttcaata gtttttttca attgcttttc aagcacattt cgcttaattt
    25321 cgggcatcat atgcatatct cttaatgtat aagatccgtt taatagatag gtagtggata
    25381 catctaatac ctttgaaagt tgtttaagcc tggtgttatt gggaagatta tagccatttt
    25441 cccaattact aacggcacct ttactggccg gtggggataa tttacctcca agttgttcca
    25501 ttgagagtcc cttttcaatt ctagccagtc taattctttg tcctgtctcc actgcttcgg
    25561 ggtatttttt tctagccaca ttttcaccac cattcatata ttttctctac tatatcacga
    25621 tttaaatatg tgtgaaacat aaaagttcaa aaaagtatgt taaaattctt gcaaatgaaa
    25681 aggtatgatg gtatattgag tttataagag gtatagaaat gtattcaaga gcctcttgca
    25741 tttttttaaa atctttgtat agaaatgtat gctaacgagg ggggataaat atggaattaa
    25801 aaattgacaa actcacgctt gatgatccag cttttaaaat tattgacgat cgagttgttc
    25861 aacaagtaga gcaaactcaa ggaacgttac tttcattatt agaaaaaaag taccagtttc
    25921 caaaatatat gaacaaggca caagctgcta agtatatgaa cgtttcatac aatactatga
    25981 tgcagaaata tgttcctaac ggtttaaagt tgattatagt tgatggagtc gtaagaattt
    26041 cacaagatga atgtgatcgc tttatggaag aataccaaaa ataaacgcaa gccgtggggg
    26101 caaagggatt aataggagga aataataatg gaatcgacac aaaataaagc aatcgaaaaa
    26161 gtattatcaa caattcttac agaagacaca gcggcagaat tagcaaactt ggaaggcaaa
    26221 gcgctagaag aaacgtttga atgtttgtac gaacaaatgg actatcaaaa attgctacca
    26281 caagaaccaa cggtaagcgg agtattgcgt ggcttgaacg atttagtaca agcagagttt
    26341 aaagaacgac tttctattga agagtatcaa gaaattttgt atcagcaagt tgagttgtta
    26401 gcgagcttgc tagggcttga actggaggac gttgaatgaa aactgaaatt tggaacggac
    26461 acattataag atttgtcgat attaatgatg aatggtgggc agtggcgaaa gatgttgcgg
    26521 aagcgttagg tcttaaacag gttactagag ccattcactc tttgcctaaa gatggggtta
    26581 ctactagtaa ggtcatcgat tcattgggca gaacacaaga tgtaaacatc atcaacgaaa
    26641 aaaaatattt accgcatggc attcaaaagc cgtaaaaaag aagccgaggc atttcaagac
    26701 tggattttcg acatcatcaa agaactacgc cagtccacag gacttgaagg ctttcaagta
    26761 ttccgcatgc tagacaaaga acaccaaaaa gaagcaatga ctaaattaag ccacgctatt
    26821 actgaaccaa aaccagtaga ctacattaaa gcgaatgtga tagccaataa agctgtatca
    26881 actatttatg gtcattccaa aatggtaaaa aagaaagata tgactcctga aatgttggtt
    26941 gatcgtgaac ctattttaga tgagacagta gaattaatga ctgttaaaga aaaatatggg
    27001 ttgcagtttt cagtgagtga gaaaatttat aatcgttctg ctgaattgca gacaacttag
    27061 gagcattaac atgatcgaga acaaagaaaa agccttatcc ataacagtct tggcggacag
    27121 gataaaggca acttaattgg gataaaaccc gatattcttt gttccgatta taccatatta
    27181 aaaaatcgga gggaaccatt atgaaacgaa acttaattat aggaagcatt ttaatcggcg
    27241 gaattttcgg cttattacca ttaattgcaa aaatagctat ctttgcagga ttagcgttat
    27301 atctcttcac tgcttatgac gagtacgact atcaattaag aactggaggg cataagtaga
    27361 tgtacaaaac aaaactatta aaccaactcg atagccttga attagaggaa attaatcaag
    27421 ggatcgctga actagaaaat aatatcggga aaacctactt tggaaattca ttcaatgaaa
    27481 aactaacagt tttgtatgta ctcaaaaaac atgcagaaca caaaataatt tgtcgagaaa
    27541 tcaacgagct aaaaaatcag attttaaccg catggttaaa cattactgat atgcaagaag
    27601 caagggtgaa aacatttaat acatgggtaa agtatcagaa tcaattaaaa ggtgctgaat
    27661 ttgttcggga tgggttgaaa tacgaacttg agcagttgaa gctcatggag gtgtctgaat
    27721 gatttatacc agtattttga aaaaggagga gaggtttatt ggcaaataat tatcaagcag
    27781 caacggaatt acaaaagtcg ggatggtcaa tttatccctt atctcctgga actaaaattc
    27841 caataaaagg ctctagtggt tggaatgatg caactaacga ttctaatcaa attaatgaat
    27901 ggtggacgga agatgcaact agaaatattg ggttaatgct tgaagatcat caaatggtgg
    27961 tagttgacct agatcaacat tctgaaacac gaaacggcgt tgagaattat aaaaaacttg
    28021 cttcaatgta tgatccattt ccttctactt atacggaaat cacaccaggt aaaggaatcc
    28081 atttcttctt taaaattcct gatggtgtaa ttatccaaca agaaacaggt gcatttgctg
    28141 atatcttcgg gcgtgatgat aaaggtaaaa gtttaagcgg gattgatatt atcactaaag
    28201 gagttccaat agcgccaact atgattattt caagaagtgt tgggagagca tatgaaccta
    28261 ttgaaaacgt atctctaaat aatattgcac cagctcccga ttggctagtg aacctactgc
    28321 taaaaaaacc gcaagaaata aagactcaat accgacccgt agcaaaaaca gggacagcaa
    28381 aaaaattaga tatgattgtt caaggcgctg gcgaaggtag tagaaacgat catttaacta
    28441 agttatgcgg ttggttacta tggcacggtg tagataatca aactttagca gaattgattt
    28501 atacagcaaa tgcgtacaat gaaccaccgc taaaagataa agaagtgaat caaattattc
    28561 gatcgatgat taaaaaagat ttgaggggga aagctcatgg ctcataagca taaattagat
    28621 agtattttag actttcccga agccagcgag cgtgaagaca atatcattga attgaaaacc
    28681 tggatgtcac gattaagatg caataaagac gatcagatta aaagtaacag tgttgtgaat
    28741 gcagaattga ttttaaccaa tgatagcaat ttagctggaa caattgctta taacgaattt
    28801 agcggataca ttcacctatt aaaagattct ccttggatca atcgtaccgc tggagaatgg
    28861 gaagacagtt ttgaggatgc cttaacggca tatattgaag aaaactacaa tgtagttttt
    28921 gatgacaaca aaatacacaa agcagtagta aatgtggcta gaaagaatgt tttcaaccca
    28981 gtaaaagaac gtatcgaaaa agttaaatgg gatcaacagc ctagactgga aacactgttt
    29041 attgaccttt taggggtaga cgacaacctt tacacacgag aagtaacaaa acgttggata
    29101 gtcggatcag tcgcacgtat ctataaacca ggaataaagt ttgagatcgt tccagttttg
    29161 gatggaccgc aagggattgg taagagtacc gttccagcat tactttacac ggatgatttt
    29221 ttcaccgatt cattagattc gctaggtgaa aagaaagatg attatatgca gcttcaaggc
    29281 aatgtaatta ttgaacttgg agaattatct tcaatgaaca aaactaaaat tgaacagata
    29341 aaaaacttca tcagtgccaa gatcgataaa attagacctc cttatggtcg gaatgtcata
    29401 gcttgggcaa ggcaatgtgt atttattgga acgtcgaatg atgggcagta tttgaaagat
    29461 gatacaggaa accgccgttt ttatcctttg ccttgtaaga ataagccaaa gatgaatccg
    29521 tttaaaacaa aggatgaata ttttcttcaa gtgttagctg aagcaaaggt gctttttgat
    29581 aaaggacaac gaatctattt tagtcccgat gaagataaag aagtcttaga aattgcaaaa
    29641 gactatcaag aggatgccaa gatggagaat ccaattaaag aagcaatcac taaatatttg
    29701 gaaatggaaa tcccgtataa atgggaagaa gctccagcgt gggcgagacg ttcttattat
    29761 cagaactatc ctgacagtaa atgtaatgag aaaaatttac agcatttcta taatggagct
    29821 actatgcaac aagagtttta tttagttgat agcgtactca ccgctgatat tttggaagct
    29881 gtttttgata aacaagcaaa ggatttattg aatggtcgat cagatgcaga aactaaaaag
    29941 attgccttga ttattagcag tattcctggt tgggaacgga agcaactgcc aaaaagaaat
    30001 aaacgacgag gattttacaa taagtcaaat gcctatgaaa ataaaaatcg agctggaaag
    30061 tacaaaaagt aacgggtagt aacgggtcag taacacctag cccgttacta gtttaccctt
    30121 actgccccaa gggatacagc aatttggtaa cggggtaaca ccttatttcc ttaaaacttt
    30181 tgaaatagtt agctaatatg ctggggaata atgttactgg attttacccg ttacctgtta
    30241 ccaaaacgct agaacccttg tccccgttgg gattgagtgg taacgggtac ctgttacgta
    30301 ggtgttacca cgtgttacga atcaaggagg aaagtaaatg ctcaatatta tttcaacaaa
    30361 caaagcaccg aactttcaat atacagatga aatggatcgg tttctaatga acacgctggc
    30421 atttagtgtg ggattggtaa cagaagatta ttccacattt gatccagaag tattaaagat
    30481 aatggaagaa gaacctgatt ggctgcagga gtcagtagcg tggtgtcaat cgctagtagt
    30541 gggatcattg gtagatagtg gcaactatga tgatacaggt gagctaatgg atgaatttaa
    30601 ttgtctgctt aacctatatg atcgagcgag acaacgagag cttacatcga atgaggataa
    30661 tttgttttta aacatccatg ataagttctt ggctttgcta ctaacagatg atgaattgat
    30721 aactaattta ttggaggtag aataattatg catatggaaa gtattagata cttcgatggt
    30781 gagaaacaca ttgagtattc aaacactcaa gaagatgtag actttattag ttttgaggaa
    30841 gataaaatca ctaaagttaa ttttaaagac ggaacgtgct tgaagatagt ttcgccttat
    30901 attgaataca aatcaaaatg ggaacaagat gattctgacg aacgagatcg ctttggttgg
    30961 taatctatgg cagttaaaaa acaatgtaat catgctggat gtaaaacatt gattgattat
    31021 aggcagaagt attgtgggaa gcataaagca aagcaaacgg caattaagcg tgaagaaaga
    31081 aagcaaagcg aaggtaaata ttttcaattc tatcaaagta gaacatggcg aaaggcttca
    31141 tacttgtatc ggttaaatca tccagtatgt gaggattgcc tagaagaagg cttgataagg
    31201 aaagctgatg ttgtagatca caaaattgaa ctaaaagatg attggaatag acggttagat
    31261 gaaactaatt ttcgttcttt atgccatgca caccataatt ccaagacagc caatgaaaga
    31321 caaagacgag aaaagagtac cctttgagtt aagggggcat agtgtgaaac ttactgacaa
    31381 tcgatgccta ctcattttgg tacaaataac cgttgtgaaa aggcataagg gtaattacaa
    31441 ataacgatta cacttgtaat cacaagtgaa aaagtatata atgaaagtag gagatttatt
    31501 gaaaaataca attgttatta ctgataaaac agggaaacag cgcaccattg agttacctca
    31561 ttttggtcaa gtgaccattc aaatgcagaa cggaaaaatc atttatatag ataaattaga
    31621 taaagaaaaa ttctgatcga aaaacgaagg aatgtgagct taattgctta cgttcctttt
    31681 tcttttgtct gaaaggagga caacatggga caccctaaaa tattacacga tacaaaaggc
    31741 aatatatcaa gtgaagaaaa agcaatccgt gtggacgcta gagaagaatt gttcaagcag
    31801 caaccattaa taaatatcac gccccctgac tggatggcag cgagcgctag aagtgaatgg
    31861 aatcgtatcg taccaacatt aaaaaaagat tatccattga gtgaagcaga ttatggttca
    31921 ttggtagcct attgtttagc ctttgctcga atgaaaacag ccgaagccga gataagaaaa
    31981 tcaggtacgt ttatcacatg cgaaaacgga gtaaagaaag ccaatccagc ggttcgggtt
    32041 caatcacaag ctatgagtga tttgaaaaaa caagccacct cactaggtat gaccttagaa
    32101 tcacgatcaa aactagcttt gaacaaggtt aaaaatgatg aacccgaaga cccgttcaaa
    32161 gagttgatga attcatgaat gattacattg agaaagtcct atcaggtaag ctgattgcac
    32221 ccaagaaaat tatccaagcg tgtgagcgtc atataagcga tttggagcgt tctaagtcag
    32281 atagcttccc ttatgtgttc gatgaagaac aagccaccaa agcgattaaa ttcattgagt
    32341 tgctaccatc tacggacggt aaagcaatca agatgttagg atttcaaaaa ttcattctag
    32401 gcagtcttta tggctggcgt actaaagaag ggaattacag gcgattcaat cgagcgttta
    32461 ccagtatgag ccgtaagaat gggaaaacgt atatcgcaag tggcatggct gccaatgcgt
    32521 tgattatgga acaagaacca gcagaagcaa ggcaagtatt gtttgtaagt aacgctttga
    32581 aacaagctaa attgggctat gatatgctgt ctaattcact tagaaacgtg gtcaagtcta
    32641 gtaagttttt aagaccacaa ctgaaaatta tgaactctaa gattcagcac ttgccctcta
    32701 attcgttcgc tatggcactg gctagtgaaa ccagcacgct agatgggttt gcaccaacaa
    32761 ccgcaattct tgacgagtgg cacgaagcaa aaactcgtaa aacgtacaac gtcattaagt
    32821 caggaatgac ccaacaaaag aacggcttat tgtgtgttat tagtaccgct gggcttgatt
    32881 taaacgttcc tatgtacgag gaatacttat tgttagatcg tgtgctaaaa ggcgaagaac
    32941 aagccgacag gtactttata gcgatatggg aattggacga tcccgaagaa attcacgatc
    33001 aagagaaatg gatcaaagcc aatccgattt ttgaaagtga agaaatcaaa aaagtaatga
    33061 ttccaaccat tcaagatgat gtgaaccttg ctttgaaaca aaataacctt aattctgtat
    33121 tggtgaaaaa cttcaattta tggagacaag cgagtgagga cagctatatg attgctgaag
    33181 actggcaggc aaccgaagta gaaccacaag atattacagg caagcccgtt tatatcggag
    33241 tggatttatc taaaacagat gatttaacta gcgtttcatg gatcgtaccg ctagataacg
    33301 gcgaacttta ttgtgattca catagctttg tagccaccaa atatgggctt caagacaaag
    33361 aaaagcgcga cggtttgcct tatcgagaac tagaaaaagc tggtgagtgt tccattactc
    33421 aattagaaag tggaatcgtg gactatgacc aagtatttca gtttattcaa gatttgattc
    33481 aagaaaatga tttggaatgt atggggattt gttatgaccc gtataacgct aattcgctta
    33541 tcagtaaagc tgaaaaagcc aactacccaa tgttagaagt aagacaagga acgattactc
    33601 taaacgttcc gacccgaact tttagagaac aagtttatga aggcaacgtt attcacaata
    33661 aaaatacgat tctcacccat gcagtgaaca acgctatttt aaaaacggat aacaacggca
    33721 ttcagattaa taaatcaaag aacagtaaca aaattgatcc aatagctgca ttaatcaatg
    33781 cctatgtgtt tgcaatggat tacttcacca caacggaagg agcgaaagca gacaatgaat
    33841 tttatacaag tgaagaattt tctttctaat tacattcata ccgttctttt acttcttgga
    33901 ttggtgtgtg tgttggttgc tatcacctta ctaacaaatg tctactatgg cttgttagcg
    33961 ctgggcatag tgcttattgg ggtagcggtc atgctaaata cagaacagaa gggaggttaa
    34021 aagatggcat tttttaaagc gagacaaaat acaacgggag atcctttcct acaacatgtg
    34081 gtatcaatcc aatcggatga ttacaccacc agctttacaa gcgttcgtgc attaagaaat
    34141 agtgatgtgt ttgcagccgt tcgaattatt gctagtgata ttgcttcaag tccgattcaa
    34201 ttggtcaaaa acaatatgcc acaagctgat aatgaactgg tgaagttact aaacgagaaa
    34261 cctaattcag aaatggatgg ttggcattta aagtttgctt tggcggtcaa tatgttgcta
    34321 aacggtaata gctttgcaga gattaaacgc aacggtgaaa aggtagaaga aattcactta
    34381 ttacctaact caagtgtaac ggttactcaa ttagataatg gtacgttgtc ttatcagatt
    34441 ggtgataaaa aaagacgtgt gaagtctagc gatattttgc acttcaaata tttcacccaa
    34501 gatggtttaa caggattacc accactttat gcgttacgtg atgaactgaa aattcaacaa
    34561 gctggcaacc gcacattgca caatttcttt actcgtggtg tcagtgggtc aggtattttg
    34621 aaggttcata agtcagattt agacggatca gcaaagaacg caattcgtga aaagtttgaa
    34681 gaagccaacg gttcaagtag tggagataat gctcttagaa cgatcattct tgatgaaaca
    34741 atggactaca aaacattaga aataaacacc gatgttttga agcttatcaa ttccaatgat
    34801 tggaacacaa aacaaattgc caaggcgttt ggtgtaccga ttgagcgttt aggcgttgaa
    34861 aatgaacatt ctagcaccgt tcaaagtaat ctccagtata tccaaagtac gctgattcat
    34921 tactttaatg tgtttgtgag tgaattagat acgaaacttg aaaccaatat ccgctttaat
    34981 tccgatcagt tactagagac tgatcctgaa acaaaagtaa aaaatgtatt ggatcaggtc
    35041 aaagggtcac ttctcacgat taatgagggg cggtcgaaaa tggggctacc ccccatggat
    35101 ggtggcgatc gtttactagc aagtttgaac tttacgtatt tagatacgtt ggagaaatat
    35161 caattaaaag aacaggaagg agttacacca gttgaataat gaagaagaaa aggaaaaacg
    35221 gctgacagaa gaagctgagc taacagccga ttctcccaaa gtagagaaag aaaatgaaaa
    35281 acaaccaaca gacggcaaaa ctatttcagg ctatgcgttg aaattcgggc aaccgtcaaa
    35341 agatttaggc ggctttgtag aagtgattac acccgaagca ttaaaagagg tggatttatc
    35401 aaatgtgttc ttattatatg gacacgatta ttcaaagcca ctagctagtg tcaaagcagg
    35461 tacgttgaaa ttgaatgtcg atgatacagg attacatttt gaagcagagc taaccgacac
    35521 gacctattca aatgatgtgt atgagaatat ctcaaaaggg gttgttgatt ctatgtcctt
    35581 tgggttcgtg cttggattag attcattcga caaaaaagaa gatggcacaa ttgaacgatc
    35641 aatagataaa atcaaagcac ttaatgaaat tagtgtcgtg accgttcccg cttatgattc
    35701 atcaaatgtc caagtaaata agcgttctta cgaatcgttt atgagtaaca accaagcaaa
    35761 acaaacaaac aatagcttag aatccacttc taaagcacaa aaggagagta ataacatgga
    35821 aaaaacttta atcgataacg aaaaaacaga attgcgtggg tatgaagaat atatccgttc
    35881 acaaggcgaa gtgcgtgatg gagtcactac tgtaaatgca gcggcagttg ttcccgaaga
    35941 agtaatcggt gaagtctttg atttgaaacg ttcaaattat aacttagctc aatatgcaac
    36001 agtaaaaaca gtatcaaatg gacaaggtaa atatccagta gcaactaacc aacaagcagt
    36061 gttagcaaca aaagctgaac ttgctgaaat tggtgatatt gacgctgaaa tgtttacttc
    36121 agttgattat aaagtagaaa ctcgtgctgg taagattgcc ttatcaaatg aggttgtgga
    36181 agattcagca gtgaatattg tacaagaggt caaagatcag ttagcgaaat tggtagaaaa
    36241 cactgacaat aagcatatca tggatttatt aaaaacattc actaagaaaa cggctgctac
    36301 gttggacgat ttgaaacaac tatacaatgt ggcattagac ccagcattaa ataaaatggt
    36361 aattctaaac caaagcggat attaccacct agatacattg aaagattcag atggacgtta
    36421 cattttacaa cccgatgtga cagcacctag tggtaaatca ttattcggta tgccagtagt
    36481 attgattgca gatacattgt ttgccaatcc taaagctgga acattcccta tgattatggg
    36541 ggatattgca caatctatct ttgttgctcg tagaaatcaa gtaacgactc aatgggaaaa
    36601 attcgattac tactcacaag gacttgcagt gatcgttcgc aacgattata agaaaattga
    36661 tcaaaatgct tcagtgtata ttgagtttac gccagttgta acaccaaaag tatagaaaaa
    36721 tattgggcgg cggtttatcc cactgccttt ttttcattag gagagataaa atggtaattt
    36781 tggatagcat aaaaaaaagt atgcggatcg atcacactat tgatgatgac tttattcaac
    36841 aattgattga tacagcaggc gaatatataa aaagtgctat tgatagtagc gcaacggata
    36901 aagatatgga taattatcag caatttgatt tggcggtgtc attacttact caacattggt
    36961 atttgaaccg tcaagaagcc agcagcgaac ggataccagt aacggtacaa gcattagtac
    37021 aacaaatgag aggtgcttat tatgccaatc attaagaatg taaatgaatt gactgagaga
    37081 gtcaagttta aaaagacaaa acgggtaaaa gatgaagatg ggcagatggt ggatagtgaa
    37141 gaaactgtat ttgaatgttg ggctaatgtg cgttcacaaa tgctaaaaga cgtgcttgct
    37201 agtgtaggta ccattcttga aggaacgttg acgtttatta ttcgatacga tcaagattat
    37261 gaactaacaa acgatatgaa agtagcttgg aaaaacaaga gttataaaat tatttcaatc
    37321 aatgaaggaa cggcgtttaa agattataca acgatcatag ctaaacagat ttcttagaat
    37381 gattacactt gtaaacgttg tgggtattta gtataattaa ggtagtaaat gaagggttag
    37441 ctgcctgaat tttgcaagac ttagctagtc gaaatctatt gttggactga aaattgtagg
    37501 tgtggttgca aacatgctgg actaagaaag atgatagatg atgaacgttc aattttaata
    37561 caggcatgct aggactgatt ggatggtttt ggcaagctga ttgtgtagaa atcaaagaac
    37621 acgtctgttg tggacgtgtt ttttttgtta taattcaaaa gaggtgattt aatggacaaa
    37681 gcagagttta caaattttta ttggaagtat tatctacatt tagaaaatga gtttattaat
    37741 accacagatt ttgtgatgct cgatgagaat aatttcaaaa cattctctat tgaatatcaa
    37801 aaactactac tagcgattgg ttcggagtgt gagattattt tcaaagaact ttgcggattt
    37861 gatagcagta gcaataaaag cataactgat tttaaagctg ttattaaatc tagcgaatta
    37921 atgtgtttgg ataatggtgt tagggttatg aattctttaa cattgacttc actaaaacct
    37981 tttggagatg ggtggcctga agatacacca gattggtgga aaatatataa cagagtaaaa
    38041 catggtcgtt cttcaaatta taaggaagca aatttggaaa atgtgttgta tgctttagca
    38101 agtctatatt tattagagga atatttgcat aagaaagtaa tactagaagg tgaagtagac
    38161 tttataagtc cagagtcaaa tctatttacg ttatcttggg aaagaaaaca ttcgtcgatg
    38221 caaaaagtga cgtttgaaaa agtggattca aattatgtag ctccactcat tgattttaaa
    38281 gaacaagaag aaatttaaaa acccccacgg cttattgaag tttttgtatg ccatattgta
    38341 tgccatttgg tttaaatcgc ttgaaaaata gtgatagata actctaattc atttagtcat
    38401 aaacgctgtt ttgataacct ttatagttct ttgatatggg tttctcattc atggcggaag
    38461 aagaagaata gaacaaaaga atgaattaat aagatataaa aaaagggatt gtgacaaacg
    38521 tcttgtcaca atcccttttt ccaaataaac gatgttcaat cagctgcccc tcggcattcg
    38581 ctcgaaaaac ggcgaaatat gaagaactat tttgccattt gtttctctaa taatcaggtc
    38641 aaaacgttga cttcacagtc tcttccttaa acggtgttct cctgccaact cctcggtcac
    38701 aagtcacaat attcaacaaa catgagaagc tgttttttga aattgttcct tgtgactcgg
    38761 agctgaacgg caggctcacg acctctttct taaacggtgt tcaaaagctt gcatctgcgg
    38821 taataagttc aaccaaacga aaattatgag gagctatttt tgtttggctg ttcttattac
    38881 ttgatgcagg acagcttttt cacaacctct tttcgtttag cactaaattt ccttaaaaag
    38941 tagaaaagct ttcgaagcct cgtttgtttc ttttactgta taataaaaga aaagagtaac
    39001 aatcgctaaa agatataaac aagagaaagg aaatacaaga tgattggtct gatttatttt
    39061 gtggtgatcg ttctggcgaa cacagttggc gctatttccg ggatgggtgg aggcgtgttg
    39121 atcaaaccga ttcttgatct tatcggcgcc cattctgttg cagggatttc tttttactca
    39181 acagttgcgg tattcacgat gtccattgtt tcgactgtgc gacaagtatc aagcgggaaa
    39241 tcactgaatt ggcagattgt cggttgggta tctagtggag ctgttgttgg aggagtagtc
    39301 ggcaacattg tattcgaagt ttttttacag ttatttgaaa acgagaagca tgtgcagctg
    39361 atccaaattt ttttgacggt attgaccttg gtttttgcgt tcttctacac caagcatcat
    39421 cagccaaaat ttcatttgac gtcatggact tggtacctta tatgtggggg ggttctgggc
    39481 tttttagcta gttttttagg tattggtggt ggaccagtca atgtttcttt gttgatgctg
    39541 atgtttgcat tgccaataaa agaagcaaca ctatattctc tatctacgat tttcttttct
    39601 caactggcaa aattggtaac gattgcttta acaagtagct ttatgcgctt tgatttatct
    39661 atgttgtttt atgttatccc cgctgctgtt attggaggtt tgtggggagc aagatttagc
    39721 agggttttgt ctccaaaaaa agtgaccttt atttttcaag taattgtcct cgtggttctt
    39781 ttgatcaatc tttacaatgc tttcgtcatc ttacaaacat taggagaatc tagactaacg
    39841 caagtcaaaa tcactgaaac atattgttct aaatgaaaaa aggagtatac tgtgataaaa
    39901 aaattcattt aaaaaaataa aaattggctg gtgatttgga tgttgcggga aatgcgttta
    39961 ggagatgcag aagcactacg agaaatcaat gcggaacaat taggatatga tgtaccatta
    40021 accgttacgg tgagacaaat gaagaaactg ttgaaggagc cagaaaaaaa ttttttcttg
    40081 gtctatgaag atcctacttc taaaaaagca gctggctatg tacaagcgga gtcctatgaa
    40141 tcgattttca gcgatccgat gttcaatatt atggcacttg ctgtgtctaa acatgctgaa
    40201 aaaagaggaa tcggaaaggc cttgatgact ggcgtagaag aagcgaaaag acgaggaata
    40261 tcagcaataa gacttaattc tgcagaatac agagtagaag cacaccgatt ttatgaacgc
    40321 atcggttatc attccgataa aatgcaaaaa agatttttga aaatcctgta aatttttatc
    40381 aaaaaatacc tggtcatcaa gccgatactt gatgatcagg tatttttttt gactaagaaa
    40441 gtaattgcat caagaaacct gagatgatca aaacgatggc accaccaagc cgattgccca
    40501 tttgagcaaa agcaataagt tccatccgat tagctgcaga taagacagct acatttcctg
    40561 taccacccat cgagttgttg cataagccag cagtaatagt tgattcgatt ggatataaac
    40621 caaacaaacg accgacaaat cctgaaacta ctgaaatcgc gataacactt gtcaaacata
    40681 agacaacaaa ttgccatgtc aaagcagaag ccaatacatt tagattcaat aacgcaatac
    40741 cgataccggc aagaacagct gctgtcaagt ttttgacaat caagttgttg aacatgatag
    40801 ctgcatcttc gtaatattct ggtaataggt ttgcgatttt acagataaca acgataatga
    40861 tcatgaatgc gtaagcatgg actttaggaa caaaatagtt acagatttgt cctaagatga
    40921 agaaagtcaa tgataccatc aaaccgacac ctaattgtgg aatggaaggt tttacatttc
    40981 taggttttcc atcgccaagg tcgccttttt ccattaattt accattaccg ttgaattttg
    41041 gcattgattg tcccgcacga gcaactaacg ctgcaccaat gatagctagg acatttgtca
    41101 ttgctgtcgc aggaatcagg cgggaaatga tagaagaagc gtccgtacct aaaacatttg
    41161 cataaatcgt tgataaaggt acagctccgg ctccaacacc accagccatt gcgggcaatg
    41221 agatatataa aacggaattt gcaaaaccat ttccgataag catacctacg agtcctacgg
    41281 caaaaaagga gacgaccatt gaaataagtg caacgggaat aaaacgaaca gaagctttca
    41341 tcaatagatt acggttcata ccaaggatgc taccagtaat caaggcagca atataaaagt
    41401 ctaaaaagcc catattgctg acaaagtttt ctgtggattt tactacatac tcaggtaaga
    41461 tcccaaatgt agcaatcgcg gcagaggcaa agatcgcaaa aacggagcca ccacctaaat
    41521 aacttttgac gatagggatt tttgttccga taaaatgaaa taaattaccg aaaatgacca
    41581 aaacagcgat tgggccaatc atattcgtcg gtaattttcc taaagccatt gcaatgatca
    41641 ggacaaggac catgatcaaa taaaggggca aacttacgcc ggtaattttt gttgaccaaa
    41701 acccttcttt tttcttttcc tcaactgcag ttgcggttgt tgttttttgt gccattttta
    41761 cagatccttt cttattctgt atcgtatgaa aaaagccaaa gagaagcggc aaaagagcag
    41821 tagttggctg gctgctgttt tgccgcagtc gcttttttct ttttagacgt catacaaagt
    41881 ttctgtatgt tcttttacta taacaatctt aatcttctaa agatttatat tcaggaaccc
    41941 atttagcaga ttcaacagct tctttgatat ccgtgatttc ttcacgattc aatttttgtg
    42001 caacaactga ttttgcgaca gtttcagcaa tcgtttgaga gaattcagtg atttttgcta
    42061 ctggaggcaa gatagctgct cctggttttg ttacatcaac gattccgcct aatgctcgac
    42121 tagcttgaga aatgatttca gcatttactc gtgtagctgt tgaagcaatc aaaccaagcc
    42181 ctaaacctgg gtacatcaac gcattatttg cttgaccgat ttggtaagtt acaccgttat
    42241 attccacatc agcagcaggg atacctgttc caactaaagc ttttccgtcc gtccattcaa
    42301 tcaaatcttt tgcttttgct tctgccaatt ttgttggatt agacaaaggc atgatgattg
    42361 gacgtggggt atgagccgcc atttctttca cgatttcttc agtgaatgct ccaggttggg
    42421 tagaagtacc gatcatgatc gttggatgga ttgcttttac aactgcttct aagtttgtca
    42481 attcatcagc attagtgaat tctgagcgtt tacgagcgaa tgggatttga ccaggagtca
    42541 atccttttgt atcttcaaac aataaaccct gtttatctac ttgatagaaa tgttggcgtg
    42601 cttcttcttc aggtacacct tgacggatca tttcatctaa aaggatgttt gcgataccta
    42661 caccagcagt accagcacca aatgtcaaga tcgtttgatc tttcaattgc tcacctgata
    42721 tattcaatcc acctaataca ccagctagta caacgattcc tgtgccttgg atatcatcat
    42781 tgaaagtagt gattttatct tcgtattttt ctaagatcgt tgcagcattt cctcgtccaa
    42841 aatcttccca atgtaataac aattctggga ataattcagt agctgattga acgaattgat
    42901 caatgaattc gtagtaagtt tcgccttcta cacgagcatg acgattccct aagtataatg
    42961 gattattcaa taattcttcg ttattagtac cagcgtcgat tgatacaggc aagacttgtg
    43021 caggattgat accagccgca gcagtgtaga ccatcaattt accgatagcg atgtcaacac
    43081 cgttcacacc ccagtcaccc atacctaaga ttccttctgc atctgtcaca acgatcaaac
    43141 gaatgtcgcg tccatctgct gcgtttttca aacttgcttt gatattttct ggttcgtcga
    43201 ttgaaagaaa tgcagcatct tgtggtttca agaaaatttc gttgtattgt tcgattgaat
    43261 ccgcaacgac aggatcataa acgacaggca tgaattcaac taaatgttcg cccatcaatt
    43321 tataaaacaa tgtccggttc gtattgaata gattcattaa gaagatacgt ttttccaaat
    43381 cggtctgttt gcttaagtat tgaccgtaag cttggacaga ttgttgttcc aatgtttgga
    43441 cagtacttgg aagcatacca gtgattccgt attttgcccg ttcttctttt gtaaaagctg
    43501 ttcctttatt tagaaaagga ttatttaata agtttagccc tgtaagcatg aatgattcct
    43561 cctaagtttt aatacaaacg aagtatagga gtcagatgag gatatagtca aaaagagcag
    43621 gtataataaa ttttatttat atgagtaata gagtcaaaag aaatcaaaat agcggtattt
    43681 gttcttttta tatcaacaat ccttgcttga ttactatgcg tatctcttat atgctaaaga
    43741 aagaaaagta gaagaataaa ggatatgtta gatagataaa tagaatctct gaagaataaa
    43801 tcacgtgatc gtgacgataa aatacactta cgtcaggtga taaaaaatac ataagtagaa
    43861 aactaagagg aggaacagaa aatgaaagaa gttcaattga tcagaaaaag cgaactatca
    43921 gaaggtggtt gcaatgcatg cggcgtagta gaagcaacaa gctatacatt aaagcttggt
    43981 gccaacaaag cgataatctc tgagttgaca gtaggcggtc ttgtggacag tctggcgctt
    44041 gctgaaggtt ttattggaga agatatttat gagatgtttt cagaaatccg tcagttaaaa
    44101 aaaggggaaa actgcataga ggttcatcat gagagcccga atgtgagatt caaacgtggt
    44161 gataatgaga tgatcttcaa taatcatgtg tccgatcaca ccgagctata tggaatcgtg
    44221 aatcaaatac taactgagct ttttggattg ggaccttatg cgttcaagga agaaaacggt
    44281 aatccaaaat taaacgaaga atggcaagaa acgattgaaa tacaaagaaa caacccgcac
    44341 ttatttcaat aggcatccac agtatttcgt aaaagggact ttgacacgac ttttgtcaaa
    44401 gtcccatcgt tttttatcta tttgtgttct aaacatttct ttcagagtag taacaactgg
    44461 aatgactgaa atgtaaacag attatttgcc tgtatcaata cggaagagta atataaagtg
    44521 tgtaaacgct atcatattat tcattcaatt ttttatagag ggaaggcatt gaaatggaaa
    44581 aaacgtatca agcaggtaca aatgaaggaa tagtggattt tatcaatatg gaagatttag
    44641 aaatagccgc ttcacaagtg attccagctg gtggttatgg ttacatcagc agtggtgctg
    44701 gcgacttatt tacttatcaa gaaaatgaac gggcattcaa tcatcagctg attattcctc
    44761 atgtattaaa agatgtagaa ttacccgata caactacaca ttttgacaaa gaaacgctga
    44821 ctgcaccaat catcatggca ccagttgctg ctcatggctt agctcatgtg aaggcggaga
    44881 aagcttctgc aaaaggtgta gcagattttg ggacgatcta tacagcaagt tcttacgctt
    44941 cttgtacatt agaagagata agagaagcag gcggggagaa agcaccgcaa tggtttcaat
    45001 tttatatgag caaagatgat gggatcaatt tggatattct tgaagtggcc aagcgtaatg
    45061 gagcaaaagc aatcgtattg acagcggatg caacagttgg cggaaaccgt gaaacagacc
    45121 gcagaaatgg gtttaccttc ccactgccaa tgccgatcgt tcaggcttat caatcaggag
    45181 taggccaaac gatggatgct gtgtacaaat cttccaaaca gaagcttagt ccaaaagatg
    45241 tagaatttat tgctgctcat tctgatttgc cagtctatgt gaaaggtgtc cagtcagaag
    45301 aagatgtcta tcgttcatta gaatctggtg ctggcggaat ctgggtatct aatcatggtg
    45361 gccgtcaact agatggtggt ccagcagcgt ttgattcgtt gcaatatgtg gcagaagcag
    45421 tagataaacg tgtaccaatc gtgtttgaca gcggtgtacg acgcggacag catgttttca
    45481 aagcaatcgc atcaggtgca gatcttgttg ctattggcag acccgttatt tatggattgt
    45541 ctttaggagg aagtacagga gtccatcaag tgtttgattt ctttaaaaca gaattggaaa
    45601 tggtcatgca attagctgga acacaaacag tagaagatat caaaaaaata aaattgcgtg
    45661 agaatcgttt tatttaagaa acaaatgacg aaaaaaatag gagtgcggca gatgatgccg
    45721 tactcctatt tttatagttc aaggatgtca ctaatagaat aatttgaaac gaaaaaataa
    45781 aattgaaata ttttttcata cttaataaaa aaggaagaat attcatgtaa acgattttat
    45841 tgtttttttc tcttttatac aaaaagggtc ataatttcaa gtaagcggat gaattcatga
    45901 ggattttttg catactgtct ctactatact taggtcacta agaaaaagga gtgatttatt
    45961 atgaaaaaga acatgattgg gacattatta ctttcaacac ttcttctagg cggcttggca
    46021 acaccagctt ttgctgaggg tcaagcgact tcaaaaggag atattacctt tactgaacca
    46081 acaaataccg tggagcctct taaccctact gatccttcaa aaccagtaga gccagcagat
    46141 ccggaaaatc cagcaactgg ccagacaggt cctttaacac tggatgtagt tcccgaattg
    46201 ccatttggta cacacgagat tgaaagcgga acaaaaacgt atcaagtaga cgcttcaaaa
    46261 aacgatacac cttatctcca agtatcagat cgtcgaggtg taggtgcaga tggccaagca
    46321 caagggtgga acgtgacagt ttcggtttca gattttgtaa acggttcaca agttttgcaa
    46381 ggcgctgaat tagattttgg aacatccact gttaaaagta cgtcagataa cgaatccact
    46441 ggaccaacta gtcaaactgt aactgggttg agtaaagcaa gtgcagcgac accaatattt
    46501 acagcagcaa aagatcaagg tctgggaaca tggctttctg tttatgatcc agcaaatatc
    46561 acactgaaag tgccgaaagc agcagctgga acattcacgg ctgatttgac ttggaacctc
    46621 gtggctggcc cagtagcata gagtatgaaa taaattcaac aaactgcggt gaaaatacta
    46681 taggtgaagg attttttacc gcagtttttg ttgaaaggat gtggtggaca atgaatcaaa
    46741 gtaaacgact gtttgtttcg tttttttcta ttttaagtct atttttcatt tttccgtcaa
    46801 tcagccaagc aaaggattca gcaggagact ttggaatcaa accggtattt ccagagaacc
    46861 aaatagataa agcaatcggt tattttgatt tattagtggc accggaacaa aatcagacac
    46921 tagaagtaat tatcagcaat tcttcagatg aagaacgaac atttgaggtc tctgtcaatc
    46981 cggcggtaac gagtgatggt ggaacgatcg attatagtca aaaaaaccca acgttagatg
    47041 agacattgcc gtttgatgtc cgtgacgttt tgctcattgc gaaaaaggaa atcaatgtat
    47101 cagcacatgc agaaacaact gtacctatag aagtgaaaat cccagcaaaa agctttaagg
    47161 gacgtgtatt agcgggtatt catgtttcac ctaaagaaga agctgaaaca gaaaatgcta
    47221 cagaaggagc acaaatcaaa aatcgtattg cttataatct cgcagtcgtt ttacaagaaa
    47281 gtcaagagac gatagaaccg gatctgaaac ttttatcagg agatttggac gaggtcaacg
    47341 caaagccaac tgttcagctt cgtttccaaa atcctcagcc taggattatc agcaatttga
    47401 tatttacgag caagatcttt tatgaaaatc agctttatat tgaaaatacg tctaatgcat
    47461 ttttagttgc accaaattcg aattttcact tgaatcttga tttagctggt gataaagcaa
    47521 aagccggcga ttaccgcgca gaaatcatag caaaatcagg cgacagtaat gaatggcgat
    47581 ttacacaaaa ctttacgatc aaaaaagaaa aagcacaaaa agtaaatgaa aattccgttt
    47641 ttgcagtaca agaacaatct ttcccatggc tttatgcagg attggttgct gcagttctag
    47701 tgttatggtt gattttgctg tttttgtggg tccacaagaa aaagaaaaaa ggagaaacaa
    47761 catgatgagg tgatttcgtt gaaaacatcg atcttattgg gagggattat tgcaagtttg
    47821 ctgttcttca gctggcaaga gccagtccaa gcagatacca aaagttcagg tacgattaca
    47881 ttttatgatc taagtccaat tccgcatcca agtggttcag tcaatggcgc atcaaaacag
    47941 cagacacagc taccgattta taaagaatct aatcaacatg gcgtattccc ggagacgaat
    48001 gaacaaaaga tccgttttct ttatttgatc attggtatag aattatggct tcttgcgtgg
    48061 ttgttgttat ggagaagaaa ggaaaaggag gatgaacatg ttgctacaaa ataaattttc
    48121 attttttcgc tccttctttt ttacttcgct ttttgtttta agtatattta tcttttcacc
    48181 ttcggcagaa gctgttggag caacgcctcc tgccatttct tatgatccat acacgtatga
    48241 aaatatcgta cctccaaatg gtggacgtag tgcagtcaat ggcgggaagc ttgtccaact
    48301 aactggggct aaccaattgc ctaaattgac tgctacttat ttgaacgatg gttcaaatga
    48361 tccttggaaa ggtggcacgg tccgttttca ttgggaaagc catgtgaaac taacaaatgt
    48421 acttgatttg gtgaatatca ctgcacaagg atttgcattt gtggcgatca tcaatttgcc
    48481 ggaacatgta tcaagcagtg atgtattaaa agcaattgac tggaacaacg cctacttgta
    48541 tattgccggt gcccccatca aggttactag cggaagttta ttatcagcag gaagccatac
    48601 cttgcgtttt gggatggggt cgtgggatac aaatactcta ttgacagata ttattgctat
    48661 tattacgcga ggcgaattca acgtggacga tatcccgctt caatttgatg ttacagctaa
    48721 tgtcgcgaag atgacagaaa acggagataa atatgatacg agtccaaaca aaattttgac
    48781 caagggtaaa ttacaacctt ctatgtctaa aaaggccgtt ttttctgttg atttttatga
    48841 ttcgaataat attatccaag atcgtatgtt caattcgatt ttagctccag ctgcaggtag
    48901 attttatcct aaattaactg gcggaaactt gaatccagga gcatatgcat tttctgcaag
    48961 tgcagagata aatacgtgga atagttacat ctcaccaagt gattcaacag gaaaatataa
    49021 tacatttgct gataccacag aatacgtgga tggaaataac cagaaccttc caggctctac
    49081 caatagtcgg acattgacga tggggctgac tgaaggacgt tttgatcagt tgccatttag
    49141 tcgctttgat cgcgtagtca actattttac aggaaaaaac gttacacaag gagcaatact
    49201 gactcatact ccagcaagca caagtggatt gaatcagaca gccaatatcg tgtacagtgg
    49261 gaaagatgcg caaggcggat tgctttctcc tgttgcttta aaggtattgg agcagtacaa
    49321 gctagacgga acggttttac caacaaactt aacagccgat agtcagtggg gaaagctcct
    49381 ttcttctgca tcgtaccaag tcacggccaa ttggtcagca actgaactaa aaaagggttc
    49441 gatccattac aggctttatt cgaaaggaac ccaaaaatta gcgacaggat ggacagatca
    49501 acttttccaa acgatccaat caaataacgg gggtgtaaat acagctactt ctacactacc
    49561 tgcattggct tcagggcaat attattttga ttatcgcttg gtggacgatg tacttactaa
    49621 catgtatccg tcttttgcgt acaaatggca gtctgaacaa gctggaatca ccactcttcc
    49681 tcaaataaca gtggctaatt ttccatctgt gagtgcagat agtcagctaa aaaaccttag
    49741 cagacaacct gagacaggag atcccctgtc tgctctatca ggagatacga ttcaagaaaa
    49801 tgtgacattc acgttaacaa aatctgggga tgcgataacc gacaaaaaaa tcacgatctc
    49861 acttcctgaa aatactactt atatcaatgg ttccttaaaa ttaaatggta caactatcgc
    49921 agataccggc attcaacaag gagtttctgt cccagcagat ctattgagca aaataggaga
    49981 taccattcat cttacttaca actatcaatt aaatactgta gatacttctt tacaatccgt
    50041 cagcatactg acaaaggcag ctgtactttc cagcaatatt acattagcag atggtgctaa
    50101 gcttcctaac ccagttgtcc aaacatctgc taaaacaatt cttgtcccca aacaagaatt
    50161 aacattagtt aatgtaccag atgattttac atttggtaac gatctcccca aaccgttgaa
    50221 gacaagttat tatgaatcaa aaggcgattt ttcttttgat gtgcgggata ctcggcttcc
    50281 aagcacgtct ccttggcaac tgacaggtac attgacttct ttatttaaga atgatcaagg
    50341 tcaagaatta agcggaacaa aattatactt caatcattct ggatcaaagc aattgattca
    50401 gcaaggtcaa aacacgttga tttatgagag tgatggaaca gctaaaggag aggttctcgt
    50461 tgactttccc gataccgatg gtttactctt agaggtaaat agcagtacga atgctcaacc
    50521 tggcgcaacg tatcaaggca tggtcacatg gaaactgaca gctgggccaa ccagctaagt
    50581 gattagaatt tgtatggatc tgtgataact gtcacagatc ctctttttca aggatgagaa
    50641 ggaaacttct gctgttaaaa tcagaaatgc ttgaaggagt ggacttggaa cttttcagtt
    50701 tgcttgaaaa aaatgaacaa cggcaaatca aacttttcca acttttatta gaggaaaaag
    50761 gagaggtttc cgttaataac gtacttaccc gcttatcagt tgatcgggct accttgaaag
    50821 aagatctgtt ttatttacaa gctaacttgg aaaatatcaa acaattggaa attgttatcc
    50881 aagaagggaa tattttatta atcagacatg gaaatatagc agcagtcgat gtttattatc
    50941 attgttactt gcgctattct acaaaatatc agatacttct ttatctgttg gaaaatggct
    51001 catttgatcg gcagaaactt gtcatggaac ttaatatcag ttcggctact ttgtctcgca
    51061 gagtgaagga attaaatgat tctttaaatg agtttcatct gaaaataaaa agcgggcggc
    51121 tgaccggtcc agaaagtcaa attcgttatt tttatttcca attattttgg tttggacgtc
    51181 cctatcttga aaacaaaaaa gaatacagtg atttatcagg aaacactttt ttagatctgc
    51241 tgcaaaaaga gtttagtttt gctattacag aagaaggacg attgaaattt gaaatatggc
    51301 tttccattat gaagaagcga atgcgctgta cgaaaaaatc caaaccagat tatagcaatc
    51361 ttcctgttgg attgtttcaa acgacacaat ttttattgga cctccataca tttttgaccc
    51421 gttacttctt tcaatatgcc tttccttgga atcaaaatga aacgttggtt ttttatcttt
    51481 tcatgatcag taactttaca ttagatacac ataactcgca tgtccaggag tttttgataa
    51541 gcgaaaaata caaagacgac gtagtagctc aattgaatcg aacgtttaaa caatcgatag
    51601 aagaatattt ctctttttat gtttttccag aagagatttc gcaaaagatt gcgttgacag
    51661 tgatgcaaaa tcacgctcat tttgcctatt ttagaggatg gatgaatatt tttggccaag
    51721 aaggattaaa acagcggtta actgaagtag aagataaaca attcctggtt ttatgtgaaa
    51781 gattggtcag acaaacattt gctattgttg attttactga agtagacgag gaaaatgccc
    51841 gctttgaatt atttgctcgc tatgcatcta ttttacggac gctgatcaag cagttagaat
    51901 tacgtctgca aattgcttat gatcttcctt atgaaaggct aatggcggat atcgtgaaag
    51961 aaatcatcca ggaacaattg ctgaatacga tcaaatatga tttagtcgaa tacgagaaag
    52021 aaaagcagta tgatatcatt ttgactagcc aattgaaaga gtacccgtca cagaagtccg
    52081 ctaaagtttt tgtattcact agcaacgaat ataattatga tttcccctat ttgaaccaat
    52141 ttttaaaaga atgttattta gaaaaattga atttaaggat gaataaaaca tgaaaatcgt
    52201 gatcattggt gcttcccatg caggcatcac agctgcccta aatctaagaa aacttcaacc
    52261 gaaagcagaa gttttactga tcgatgaaga tcataaagat ggactaggct atgtttcaaa
    52321 tggaatcaac ctttatttaa aaggaaagat acgtagttta gctgaagttg cccataacat
    52381 gcggactctt caagaaagtg gggcaaaact gatcaccgaa tggagagtaa ctgaacttga
    52441 tccagacaaa catcagctca tcctcgcatc caaagaagga aaaaacgaga ccataaccta
    52501 tgataaatta atcgtagcga caggttcctc tcctgtcacc ttgtataaac aaattgaagc
    52561 tgaaaatgtc tacacctata aaaatctagt gcaatctaag caagtacttg ccgctttaaa
    52621 agaagcaaaa gaagtagtga tcttcggtgc tggttacatt ggattggaat tagcagatgc
    52681 tttgagaaat aaaggataca ttattcatct agttgattat atgccgaatg ttttatcaag
    52741 atatttcgat aaggatatga tcaacagttt tcaaaatcag ctgcaaacta agcaaataaa
    52801 tttttatcca aatgaattct tgatcgattg gaaaaaatca gaagagaaag tagtatccgt
    52861 acaacttctg tctcaaacaa taaaggcaga tatggtcatt ttttctgccc aaactcgtcc
    52921 taatacgaca ttgttaaaag aaaaagtggc tttgtatgag gatgagactg tgatagtcaa
    52981 tgaatatctg cagacaagtg atccggacat ctatgcgatt ggagatatcg tcccagtcag
    53041 ttttgacaaa aataaacggc atctcttttt acctttagtt actcgagctg tacatatggc
    53101 aagagcagta gcgctaactt tgagtggtca gccaaccgct tacgacctca gacaaaagat
    53161 aacagcagca gtcattatag actacttttt aggaactgta gggctgacag aagatgaagc
    53221 gccattcttg gaacaaaata ccggttcgtg ttcaggagaa tttgatctat ttccacaata
    53281 cgacgaagaa aacaaaacag taaatgcaaa gctgatctac catccggata cattagaaat
    53341 aattggcgga cagctgataa gccaagaatt cttattatca gatttgaacc tgcttgcgga
    53401 tatcgtgaaa cacaaaacta cgattccaca gctagcagtt gaggattttg gatttctgtc
    53461 tgaatacacc tctcgttttc actacctcaa tgagttagct tttaaagtac tagctaagag
    53521 agcaaatagc aatagagtag ataaaagact gtgagaaata tcacggtctt ttattgtgtg
    53581 gggatataat aaatattaat caatcgagaa taaaaaattc atataatgaa atagcggaag
    53641 aattttttga gcataatatt cgccaaaaat ttatcaagtg ataaaattgt tgtataaaat
    53701 gattatttca ggaggaggag ttaaatgttt ttagcattaa atgaaattat gcattcaaag
    53761 ttacgttatg cgttagttgc aggggtgatg tttttgatcg cctacctggt attttttttg
    53821 actgggttag cttatggctt agctcaagat aatcgaacag cagtcgacaa gtgggaggca
    53881 gattcgattg tgctttcgaa agatgcaaat tcaaatctag ggatgtccat gattactaag
    53941 aagatagcag aagaagtcga aggtgggaaa gtggcgtatc ttgcccaaac acctggagtc
    54001 gtgactagta aagacagtac agaagaagga aaaatcaatg ttagcttctt cggtatcgat
    54061 aaaaatcaat tcatcatgcc caatcttgtt gaaggaaaag cttttgataa cgatgatgag
    54121 gcagtaggcg atattagttt gaaagaagaa tatggtctag ccataggaga tacagttaag
    54181 ttatctggta gtgacaagac gtttaaacta actggcttta cggatcatgc caaattcaat
    54241 gtctcaccag ttctctatac aaccatcaat gcttaccaag aaatccgctt cgaaaaagaa
    54301 gatacaagtg aaaatgcacg gattaacgca atagttgtca gaggtaaaat cagtaattta
    54361 cctgaagatt tagaacagat caaaatttct aaattcatca atgaattacc tggatataat
    54421 gcgcaagtat tgacttttgg atttatgatc ggatttttga tcgttattgc ggctattgtg
    54481 atcgggattt tcatttatgt actgacgatg caaaagatta atatctttgg tgtaatgaaa
    54541 gcacaaggga tcacaggagg atttattgcg cgttctgtag tggcacaaac attcatttta
    54601 tcatttgttg gtattcttct tggtctatta ggaacagtgg gaacatccct tgttctacct
    54661 gatgcggttc cattccaaag caattggctg ttttttggcg tcattagctt gttgatgtta
    54721 gtagtagctg tgttaggcgc actattttct gttcggacaa tcgtaaaaat cgatccgttg
    54781 aaggcaattg gataagaagg aggaagaaca tgaaagcaat agaatttaaa gaagtagaaa
    54841 aaagtttcca agatggcgac caagtagtta aagcattgaa agaaacaacc atgagtatcg
    54901 ataaagggga atttgttgca atcatcggtc cttctggttc aggtaaaagt acttttttga
    54961 ctttggcagg cggattgcag acgcctagca gtggctctgt atttatcaat gatcaagaat
    55021 tcagtaatca aaaagaaagc gaacgcgtta aactacgttt taaagaaatt ggctttattt
    55081 tgcaagcttc caatctggtt ccttttctga cagtaaaaaa gcagttatta ttagtagata
    55141 agattaaaca tgaacatcgt caaaaagaag caaacgaatt atttgagcag cttggcgtgg
    55201 ataagttgat caacaagtat ccagaagaac tatctggtgg agaacggcaa agggtcgcaa
    55261 tcgctcgtgc attgtacaat gatccgtcca ttattctagc agatgaacca actgctagtt
    55321 tggattcaga aaaggcacga gaagtagtcg atatcttggc aaaagaatca aaagacaaaa
    55381 ataaagcaac gatcatggtt actcacgata cacggctgat tgacaaatgc gatagaatat
    55441 ttatgatgga agatggtgta ttgaaagaaa aagaataacc aaaaagcagg tggaaaaatg
    55501 gtccatcgat acaacaaaga tacacagcaa aaaattttga aagaagcgac tgacttgttt
    55561 atgtccaagg gatatctcgg cacttcaaca agagaaatcg cacaaaaagt tgggatcacc
    55621 cagcccaatc tctatcatta tttcggtgat aaagaaaaac tatatacggc agttttagaa
    55681 aaccacttaa aagatgttgg aaaagctttg agagaaatcg ttcaaacaag cgaaacggga
    55741 tttcaagtaa ccttgacaaa aatggcgcag tttttgattg agacacatct ggtcgatttg
    55801 tttctgatgc ttcacgatct tgaaagcaat ctttcaaagg aaacaagaga tcatcttttc
    55861 tttctatgga agaaaaatta tcgtgaacct tttgaagaaa tcttttcagc aaaccaatct
    55921 gtgctaagaa atgggatttc aaaagaaatc gcagcaaggc attttttctt agtgttagca
    55981 ccttacatca ctcggtcgaa aagacctgtg gaagaaccgc taaatgtggc gcaattgatc
    56041 gatttatatt tgaatggtgt attaggagaa gcgtaaagct gttatatagg gagaaaatag
    56101 ttaggtgaag aagcaagata tgacgaagat cgttatatct tgcttcttta tataggtagt
    56161 ttttcataaa gacaaatttg ttattctctc atgattatca gggtatattt ttatttatta
    56221 tgtcggtgtt ccaggaagtg ttttaattta ataacgaaga aattaattgt ttgtagaatg
    56281 aaggaaacta tttattgggt agatgaatca ccacgaatag tcaaaaggag atttcaggat
    56341 atgccaaaaa tgaaaaaaat cattttagca gtaatgttat tttctacggt tatatctttt
    56401 acagcccctt ttttgcttac ttaagctgat tttaagtttt ggcccttagt ctggatctac
    56461 gaggtgagag tcaagaatga aaaaaataat tggagctttt gtatttgcaa tcattatcat
    56521 tattatctgt tttcaaatag atgtttttgg tcaaaaaaag atagttggac aaacggtcag
    56581 cagtacgaac agtacggtta caaagacaaa ggaatcattt gatgatacag aacaagagac
    56641 attaatcaaa atcggaaaaa atgaccgtac actatataca gatgactcgc taactacccc
    56701 acttgcaact ataaacggcg gagagctggc ggagtttctt tctgaaacaa aaaatgctta
    56761 tcagatcaag acaaatgatg gctatactgg atatttagcc ctcgaagatg gaaccaaagt
    56821 cacaaagaat atacaaacaa aaccggaaga gctatctgat gcagtgattg tattagatcc
    56881 tgggcatggc ggaaatgata ctggggcatt aagcaatgat gaacaaacag aagaaaaaga
    56941 tataacatta agtacagcca taaaagtaaa aaaggcgcta gaggatgctg gagcaacggt
    57001 ttatttgacc catactacag atgaattagt acagcttgga gatatatgcg actacagtga
    57061 agaaaaaaag gcagatgtct ttatcagttt acacgcagac tctacggaat atgcgaatga
    57121 agcaactgga atcacgacat attattacta tggacaagag gaaacattag cacagacaat
    57181 tgctgactcc ttcactgatc tgcctctgga ttcaagagga atctccactg ggaactatca
    57241 agttcttcga gaaaatcttc aaccttctat tttaatcgag atgggttata tgaataatga
    57301 tagtgattta aaggaattag taacagacag ttatcagcag cagattgctg caagtttgac
    57361 acaagcattg accacttatt ttcagtaata aataagccca gccaaacaaa aatagctcct
    57421 catatttttc gtttggccgg gcttattatc gcagatgcaa gcttttgaac accgtttatt
    57481 tttttagtag cttcaaagct ttttctgtat ttgcaaagtg aagtttggca tatttttcaa
    57541 gcataggcat gccgcctttc tccaatagtt ttgccgctac ttggtcagat ttcgttccag
    57601 ctcctgatgg aagggtgatt ccagcttcag ctgatgcttt gtgtaactct tccaaaaagc
    57661 tggcacgact gatgatagaa gcagcagcaa cagctaaatg atattgttct cctttagtaa
    57721 cgaagtacag tttttcttgt aattgatttt tttctaaacg cgcatatttt ttaaaatttg
    57781 cttccggcgt aaactgatcg atcaaaatcg cttctggttt ttctggctcg atttttttta
    57841 gcagcaaatg gatcgcttga ttgtgcaaag cgactttcat ccgaacggca ttgtattttg
    57901 gttggatctc attgtatttt tctggagata agacaagcag tttatagggg atggtttttt
    57961 ttaagatgtc agcaagctgg atgatttggc tatcttttaa ctcttttgaa tctctaacac
    58021 caagtttacg taattctgca agctgatcac gtttcacata agcagcacat actgttagcg
    58081 gaccgaaata gctgccattt ccgacctcat cactacctaa aacggataat tgactgatat
    58141 tttttggcaa agaagaggct gtttttgctg gcttggcttg tttatcctgt gcctcccccc
    58201 atttatcagc ttctattgct gcattcgtac cttgaaacat cacttttccg gatgtataag
    58261 cggtaatggt cgtcccgtct ttttttgcag caaagctagt ataaggaatc tgtttattta
    58321 acaaataaga gcgatagtat gctttcattt gactaagttg gtcgtttgtt gctttgataa
    58381 tgacgttgct cataaaattc tcctcgtttt tatacataaa actataccgc aactcacgac
    58441 ttttcgtttt ctaagagatt gcgagacata gcattttttt aaagtttatc atataatagt
    58501 taagtatgtg aaaaatattt gtggagaacg gtgaaagaac caacttcatg gtacactaaa
    58561 taaaactttc atcaaatagg aggaaatgat tcgtatggcg cacgaaaaaa cacgctacaa
    58621 agcggtgatt gccaaccaaa catatactat tatcggacgg gaaaccaagc atcatatgga
    58681 tatcgtaacg aagctgatca atgaacagtt agcagaattg aaacaattat caccacaaat
    58741 ggataatgaa caagcagcaa tcttgatggc tgtcaatgcg ctttcggatc aattgaagaa
    58801 acaagaaaga atcttggagc tggaagaaga aacagcagaa ttaaagaaaa aaatgatcaa
    58861 atttacagag cttgaaaatc gtgtgaaacg cattgaagca atcgagaacg aagctcggga
    58921 agtattgaaa gaaaatggtc aagcagatca taccattaaa aatcatgtgg aagcacaaca
    58981 gattttaaac gagaaacgaa aaggacaaat taaacaaaaa tcttctaatt aagatccttt
    59041 tatcaaaata agaaaaattt aaaaaaagtt agaagagaaa ggaacgtagc atgctgagtt
    59101 tgctgatttt atttttattg ctaattgcgt ttttttctgg agcacgtcgc gggtttgctt
    59161 tgcaaatcat attcgcgatt ggatatgtat tgtcatttat tgctgcccag catttttaca
    59221 aaccattagc aagtcatttg gaattataca ttccataccc agctgtcacg ccaacatcaa
    59281 agttagcatt ttttgatcag gtatttgcct ttcacctaga tgaagctttt tatgcaggaa
    59341 ctgcttttct gattattttg ctaattggct ggctgcttac ccgatttgtg ggggtttttg
    59401 tccatggatt gacgtatgtg ccgatcttga gacaggtaga ctggattgca ggaggcatcc
    59461 tgtcactaat catggcttac gttactatat ttttgatttt gcagttactt gcctttgttc
    59521 cattagatgt ggtccaaaat caattcggcg agcatagttt cgctcgcttt atcgttgaga
    59581 gaacgccatt tcttaccaac aagatgtatg agctttgggt aacgaatatt ttgaattaag
    59641 gaaaatagtg ctggaaaata tttcgttttt tgtacagagt agattattta agaaggctgc
    59701 gtagcgtatg tccggcacag ctcttttttc atagtgaaag ataaggtgat aaaatgaata
    59761 accgaatcct tgaaacattg gaatttgaaa aagtcaaaca gatggtcaga caatttgtag
    59821 tgactgcaca aggaaaagaa gaactggcag aattggtacc agtgagcgag gaacaaacga
    59881 tcacaaactg gcttcaagaa actgaggatg gattgaaagt gcaacgtctg cgaggcggga
    59941 tccctatacc taaacttgaa aatatccgtc cccatatgaa acggattgag atcggagcag
    60001 acttgaatgg agtggagctt gcacaagttt ccagagtatt gtctacgaca agcgaattga
    60061 atcgtttcat tgatgatctt tatgacagtg aaatcgaatt tgcacgtttg tatatgtggg
    60121 cagatcagtt agtcacgatc cctgttttga gccgccgttt aaaggaagca atcgatgaag
    60181 acggacgcgt gactgatgac gcttctcctg agttaaagag cataagacag aacattcggc
    60241 gaagtgaaca agctgttcgt gaacagttag atgggatcgt ccgtgggaaa aatgcaaagt
    60301 atttaagtga tgcgatcatc accatgcgaa atgaccgcta cgttatccca gtcaaacaag
    60361 aataccgcgg tgtatttggc ggtgtagtcc acgatcaaag tgcttcagga cagactttat
    60421 ttattgaacc aaaacaagtg gtggatttga ataatcgttt gcgtcaatac caaatcgctg
    60481 aacgaaatga gattcaacgt attttaagtg aactttctgc tgaacttgtt ccccatcgtc
    60541 aagaaatcat ccacaatgct tatgtgattg gtaaaatgga tctgatgaat gcaaaagcac
    60601 gatttggtaa agaagtaaaa gctatcgtgc cgggaatcag tgaagacaat catgtcgtct
    60661 tgaaacaagc aagacatcca ctgatcgatc aggaaaaagt agtttctaat gacatcacaa
    60721 tcggaaaaga ctatcaagcc atcgtcatta caggacctaa cacaggtggg aaaacgatca
    60781 cgttgaagac tttaggtctg ttacaactga tggggcaagc aggattaccg attcctgcag
    60841 gtgaagaaag tcaaatcggt atttttgagg aagtatttgc ggatatagga gacgaacagt
    60901 cgattgagca aagtctatcg accttttctt ctcatatgac gaacactgta gatattttat
    60961 caaaagtcaa tgaaaaaagt ctggtattat ttgacgagct cggtgcagga acagatccgc
    61021 aagaaggtgc tgctctagca atcgcaatct tagatgatct tggtaaaaag tctgcttatg
    61081 tgatggcaac aacgcattat cctgaattga aagtttatgg atataatcgg gcaaatacga
    61141 ttaatgccag catggagttt gatgtggata cattaagtcc aacgtatcgt ttgctgattg
    61201 gcgttcctgg acgaagcaat gcgtttgaaa tctctagccg attaggtctt gatacagaag
    61261 ttattgatga agcgaagcag ttgatgaatg atgagagcca agatttaaac gaaatgatca
    61321 cagatttaga gaatcgccgg aaaatggcag agaccgaata tctggaaatg cgtcattttg
    61381 tttctgaggc acaagaactt cacgatgatt tgaaagaagc ttacagctat ttctttgaag
    61441 aacgtgaaaa agaaatggaa aaagcaaaga aaaaagcaaa tgaagtcgtg tcagaggcgg
    61501 aagaaaaagc agaaaaaatc attgctgata ttcgtaaaat gcaacagcag attgggcaag
    61561 gaaatgtgaa agagcatcag ttgatcgatg caaagaccca attagcaaat ctacatcaag
    61621 aagaaacttt gaagaaaaat aaagtattga agaaggcgaa agaacaaaaa acgttgaaac
    61681 cgggagacga agtgttggtt acgacttacg gccaaagagg gacgctttta cgtaaaaacg
    61741 ggaatcagtg gcaagtagaa atcggtatat taaaaatgaa tgtgtccgaa gacgagctga
    61801 cgcctgttgc tcctcaaaaa gagccgacac aacgagttat ccatgctgta cgatctgaat
    61861 ctagtagtca cgtgccgaat caattggacc ttcgaggaaa acgatacgaa gaagcgctaa
    61921 gtgaggtaga tcaatatttg gattctgcca ttctagctgg ttatcctcaa gtgactattg
    61981 tccacggaaa aggaacaggc gcacttcgca agggaattac tgattatctt aaaaaccatc
    62041 ggagcgtcaa aagctttgag tttgcaccgg caaaccaagg cggaaatggt gcgacaattg
    62101 ttaaatttaa gtaaagctta ctaaaggtaa gcagatggtt cgctatccaa gtatagaaat
    62161 gctatactag tcatagattt taaaaatgga ggtctttgat atgtcacaag taattacaga
    62221 taagacattt aatgaagaaa cagataaagg acttgtattg attgattttt gggcaacttg
    62281 gtgtggtcca tgtcgtatgc aagcaccgat tttggatcag ttggaacaag aatatgatga
    62341 agaagaattc agaattgcta aaatggatgt ggatgaaaat ccagaaactc ctcaacaatt
    62401 cggtatcatg agcattccaa cattaatgtt gaaaaaagat ggtcaagtag tagaaaaagc
    62461 tgttggtgtt cattcaaaag aacaactacg tcaaatgatc gatcaatatt tataaaaact
    62521 tacagcaaaa ataagctgag ggtgggactg aatccagttc cgtcctcggt ttttttgcgg
    62581 ttgagttaag gttattcaaa ttaatatttt ttgagtataa ttacgttgtc aggctggatt
    62641 gtttttttga cagacatgct ataataactt taattgaata tgaaggggag taccagatga
    62701 acgaacgaat caaaaacaaa ttagctttac ttcctgatca gcccggctgt tacttgatga
    62761 aagataaaaa tggaacgatc atttatgtcg gcaaagccaa aatattaaaa aatcgtgtcc
    62821 gctcatattt tactggtagc cataatacga aaacggaacg gcttgttagt gaaatcgaag
    62881 actttgaata catcgttaca gaatcgaata tcgaagcatt gctcttagag atcaatctga
    62941 taaagaaaaa tgatcctaaa tacaacatta tgttaaagga tgataaaacc tatcctttcc
    63001 ttaagataac gaatgaaaaa tatccccgtt tggtgattac tcgtaaggta ctaaaagaca
    63061 aagcactgta ttttggacca tatcccgatg tcggtgcggc gaatgagaca aagaagatcc
    63121 tcgatcgatt atttcctctg agaaaatgca aacctagtca gacgaaggag ccttgtcttt
    63181 actatcattt gggacaatgt ctgtgtcctt attatttcga cgtagatccg aaagtctata
    63241 cgaaaatcgt tgatgaagtg aaaaactttt taaacggagg acacgaaaaa atcgagtcag
    63301 agatccatca gaaaatggaa aaagctgctg aaaacatgga gtttgaaaaa gctgcagaat
    63361 accgagacca gatccgtgcc attgagacaa tcatgactcg ccaaaaaatg acaaatactg
    63421 atttactgga tcgagatgta tttggttatg cgattgataa aggatggatg tgtgtccaag
    63481 tgtttttcgt aagacaagga aagctaattg aacgagatgt ttcaatgttt cctttctata
    63541 acgaagcaga agaagatttc ttgacttacc tcggtcagtt ttatcaggaa aatgagcatt
    63601 tcattcctaa agaagtcttg attcctgata cgatcgacaa gcccagtgta gaagctttgt
    63661 tagctacgaa agtgctgcaa ccaaaacgag gagaaaagaa aaaattagtt gaattggcga
    63721 gtaagaatgc ctcagttgct ttgaatgaac ggtttgatct gattgccaga aaacaggaac
    63781 ggacgatcgg agcagttgaa cgtttaggaa acgcgatgaa tatcccgacc ccaatacgta
    63841 tcgagtcttt tgataactct aatatcatgg ggacgaatcc tgtggctgcg atggtcgtat
    63901 tcattgatgg aaaaccggca agaaaagaat atcggaaata taaaataaaa acagtcaacg
    63961 gaccggatga ttatgcatca atgagagaag tcatctatcg cagatattcg cgagtgattc
    64021 gcgaagggtt gcctttacct gatttgatct tgatcgatgg aggaaaagga caagtagatg
    64081 tggcaaaaga tgtcttagaa aatcaattag gaatcgatat tcctattgct ggcatggcaa
    64141 aaaacgataa acataaaaca aatgagttgc tattcggtac agatttggct gtagtaccgc
    64201 ttgagcgaaa ttcaccagag tttttcttac tccagcgaat ccaagatgaa gtccatcgat
    64261 tcgcaatcac tttccatcgg cagctacgca gtaaaaacag ttttgcttcc cgattagatg
    64321 aaattgaagg gttaggacca aaacgaaaaa aaatgctgct taaagagttc aaatcgttga
    64381 aaaatatcac agcagccacg acagaagaat tacaagaaat cggactgccg aaaaatgtcg
    64441 cacaaaacgt atttgataaa ttacatcagg aatgaggaaa gggcacttta caaagaaagt
    64501 aaagtgtcct atcattttat gtacaacaaa aagagcgttt acgttttgtt ggtaaataat
    64561 aaatcgctta cgtctggtgt ctaaggatat atgtgatttc ttgaagaagg agtgaagaaa
    64621 tgttagaagt caaggatctt gttaaaactt ttgggaacta cacagctgtc gatcatgtct
    64681 ctttccaaat ccccgatgga aaaatcatgg ggctaattgg ccaaaacggg gctgggaaga
    64741 caacgacttt tcgtttgatc ttggattttt tgacacaaga ccaaggagaa gtgttatgga
    64801 atggccatca tcttggggag aaagattatg atgtcatcgg ttatcttccg gaggaacggg
    64861 gactttatcc gaaagtaacg attcaagacc aactgcttta ttttgcagaa cttagaggga
    64921 aatcaagaaa agagattgaa ccaaaaatcg acttttggat ggaaaaattt caggtaaaag
    64981 ggaaaaagac ggataaagta aaatctttgt ctaaaggaaa tcagcaaaaa gtccagctga
    65041 tcgccacgtt aatccatgaa cctaaattga tcattttgga tgagccattc agcgggttag
    65101 atccggtaaa tgctgaattg ctaaaagatg gaatattgga attgaaaaaa catggttcat
    65161 gtgtcatttt ttctagccac aatatggata atgtagaaaa aatctgcgat catttgatca
    65221 tgttgcgaaa cggaaaaatg gttttgaatg gaaaagtaca tgagattcgt gaatcatttg
    65281 gccgtacgaa gttattcttg gaatctggtt taagccaaca ggaagtagaa gaaatcgaag
    65341 gcgtgaaaaa agtgatcctt catgaagaca atacgttaga gattacctta gaagatcctg
    65401 aagctgggaa agaaatcttt actcgtgcga ctcagttcgg ctacattcct atgtttaacc
    65461 agcaaccgcc aactttggaa gagattttca aattgaaagc aggtgaaatc gatgcgtaag
    65521 ttttggatta ttaccaaaga tgtctattta aaaaatgtta agtcgatttc tttcttgatc
    65581 atgatattag tcccttttat catgctgggt atcatttatg tagcaggaaa ctttgctcaa
    65641 caaaatagtg atgtcaataa aatcggcgta ttggctgata atgagcaaat cagtcagcaa
    65701 atgagccaag tcaaaacaga tgattttgag ttcaaagcag ttgattctga agaaaaagca
    65761 caaaaagagc tttcggaaga aaaaatcgat gcgtatttag tagtctcaac tgagaatgat
    65821 aagataaatg ggatgcttta tagcgaaaat tcattaggac aaagtacaca actactgata
    65881 caacaacaac tgactggctt gcaatcgatg cttcgagcga gtcaattagg catttcacca
    65941 gaagaagtag ctagcttgag ccaacaagca agtttgactc gacaaaaagt caattttgat
    66001 tcaaaaggaa aaatgacagt cggagaagat aactcgacag tccaatacgt cgtaagttat
    66061 gtagcaacga ttgtcttgtt cattattatt ttgacgtatg cgcagatcat tgcacaagag
    66121 atcgcttctg aaaaaggcac gcggatcatg gaagtgatcc tgtccagtac gactgcacaa
    66181 aagcattttt acggaaaatt aacaggtgtt ttacttgttg cattaacgca aatggcatta
    66241 tacggtatta tttttggtat aggatacagt caattcaaag atatggaaat ggtcaaaaat
    66301 atattagacg gtatctcatt ggataacttg attggaccat tcttgtggta ttctcttcta
    66361 ttcatgatct ttggcatttt gattttttct gttttagcag ctttatgtgg ctctttggtc
    66421 aacaaagcag aagatacagc aaaagcaatt ttgccagtga cttatctatc tctaggcgga
    66481 tacatgcttg gattgatact aggagcttca gatccaaaca atatcgttat tcggatcact
    66541 tcgtatattc cattcttatc ttcatatatt atgccggttc gcttagcaaa tgagacagtc
    66601 ggtatgggtg gagcattgat ttccttgttg gtacttgtag ccgtaacatt cttattgatg
    66661 atcttgtctg caaacatgta caaatcgaat gtactcgttt atagcgaagg cggattatgg
    66721 agttcattga aacaatcgat ttcaattatg aggaatgaac gaaaaaaagc ataaaacaat
    66781 cctaaaatac agaaggaagc tggacatcta ttgttcccag cttctttttg tatagaccta
    66841 ttttatttat tttactatat tggatatata ttcattgaaa atagtgtata acaactttat
    66901 aatattgagt ataccaattt ttggaggcgt tttaatggac cgttatttag atttactttc
    66961 agaaaaattc ccctcaacgg aggatgtcat cacggaaatc atcaatctcg aagcaatttt
    67021 gcagcttcca aaaggaacag agttattttt gagtgatata catggcgaat ttcctgcttt
    67081 tgatcatatt ctgcgtatcg gttcaggaaa tcttaaagaa aagatcaaag accttttttc
    67141 tgaacaaatg acggaaacgg atatcagcca attcactatt tttacggctt atccagaata
    67201 tgcattaacg actgattggt acaaagaaca agataaatcg cagctgatcc atcatttgat
    67261 cgatctttta cggtttacga cagtcaaata tacccggtca aaagttcgaa aaggattgcc
    67321 gaaagaatat agctatatca ttgaggaact cttgtatatc gatgaccgta tcaatggaaa
    67381 aaaagactat gtaaaaaaaa tcattgaaca acttgttttg atgaaggaag aagaaaaatt
    67441 tttaacgaaa cttgcacaga cgatccaaaa atctgtcatc gatcatttgc atatcgttgg
    67501 agatattttt gaccgaggaa ctcaagcagc caaagtgatg gatcgaatca tggatttttc
    67561 ttctgtagat atccaatggg gaaatcatga tattttgtgg attggtgcgt attgcggctc
    67621 tgaggcatgt ttactgacac ttttacggat tgcagcacgc tataattatt tatttgaatt
    67681 agagaaagaa tatgggttga atttacgtcc attgttttct tttgctcatc aaacttatca
    67741 gaagaatcca gtatttgcgc ctaaaaacag taaaaatcta tccgaaagag agcaagaaga
    67801 attagaaaaa atccatcagg cactttctat ccttcaattc aaatcagaac atcagttatt
    67861 ggatcgtcga cctgaattta aaatggacga tcgtaaattg ctggaaaaaa tcgactatga
    67921 agcatcaacg attgatttag aaggtcagct ctatgagttg aaaggaactt gttttcaaac
    67981 gatccagcca gatgatccaa aaaaattctt ggaagaggaa caaaaagtga ttgatagttt
    68041 gatgtattca ttccagcact ctttgagaat gcaaaaacat atgacgtttt tgatcgaaaa
    68101 agggggaatg tatctcacga acaatcagca tgtgctgttt catggctgta tacctgtaga
    68161 cgagaaggga cagctcttag catttgaatt agaccaatct tatcgcggaa aagaactgct
    68221 gggctttttt gaaaaacaga taactgaaag cgcaaaagat ctgactgcaa aagaagattt
    68281 agcaaccgat ttgatttggt atgcgtggag tgggccattt tcgcctttat tcggcaagag
    68341 taaaatggcg acttttgaac gttattttct cacagagcct catacgcacg tcgagaaaag
    68401 caatccttat tatcacttgc gagatgaaga gtggttcagt gaaaaaattt tagcagaatt
    68461 cgacacaaaa gaagaagggt cagcaatcat taatgggcac acgccagtaa aagtaaaaaa
    68521 aggggagtca ccgatcaaag caaatggaaa agtatttgtc attgacggag gtttatccaa
    68581 agcttatcaa aaaacgacag gaattgccgg ttattcacta ttatgcaatt cttatggttt
    68641 ccaaatcgtg acacattatc cgtttttgcc tattgaacaa caatttgcaa aaaaatccga
    68701 ccaaacaaat gtgaaaaaag taatcgaaca gcaactacca cgcaaactca atcgtgatac
    68761 aacaaaaggt atggaactgc agcagcagat tacccagctc aaaaggctgt tggactatcg
    68821 aaaggacgat tgagaagaat tccagtgaac attgttccat agactggaga ctgagacaaa
    68881 agtcgtgtcg cagtctcttt ttccggataa acggtgttct tctgccaact cctcggtcac
    68941 aagtcacact attcaacaaa catgaaaagc tgttttttga aattgctcct tgtgactcag
    69001 agttgaacgg caggctcacg acctcttggt ataaacggtg ttcaaaagcc agtttctcgg
    69061 tattaagcca aatttatgca aaaatgtgaa aagccatttt cacaaaattt ttcttaatac
    69121 tcgaaactaa acagcttttt cacaaccccc tcagaaagtc tattaaaatt cattcacaaa
    69181 gaaatcgaat aagcgctgtt cagatggatg tacacggtgg gtaagttccg gatgccattg
    69241 aacaccgagg attttatgca tatcatcttt gctttgaacc gcttcaatga gtccatcgtt
    69301 agaaaaagcg atggctttta ataaaggtga aagatctttg actgcctgat gatggtaaga
    69361 attcacaaaa tatggatcgg ggagaaggtt gcttaacaaa ctgtctgaaa caatcttgac
    69421 ttcgtgagtc gtaaattgcg gggcagtggg ctgttgttca tgttttactg accactttgg
    69481 atataatgaa agatcttgat aaagtgtacc ttccaaaacg acattcaata gttggagtcc
    69541 ccggcagact gcaaaaattg gttttctttg tttcaatgct tccaagatca gtgcctgttc
    69601 aaattggtct cgttgaatat ttgtttccgt tagctttgga tgaggatctt ccatgtaaag
    69661 ttgtggactg acatcttgac cacctgcaag aataagtttg tcgatttgtc caatcagttg
    69721 aggagctgaa tctggagaca tgactggcag aataagaggc aatccttgag catctaaaac
    69781 tgcatccacg aatccttgcg gtgtatagct cacttgattt ccttggaaag tatctgttgc
    69841 tcgaatcagt tgatttccgg caatgccgat gattggtttc atttgtattc cccctaaagt
    69901 agacttattg taacaaggat ttaaaaaaaa ttaaactatg taagacttgc ccctatactt
    69961 atctgttctt gctgtgttac tatctgtaaa gagaaatttc gtgtgaaatt catgacgata
    70021 gttgaaaata ttgcttaatt tgttataata aaaagtaagc agaaataaaa aggttgggcg
    70081 taagcttcaa ggatctagtt cttcaagggg tgagaaggac attttattga aggggcctgc
    70141 ctatactaag ttatctttag aggtacatac tttcaatgaa aaaagaaagc atgtacctct
    70201 tttttaaatc gaaaggaagt gaagcaagtg ttagaagctt tggaacaaat ccgaaaagca
    70261 gaagaagaaa atgaaaagcg tgctcaaaca ctgcggcagc agctgaagat ctatgcaaca
    70321 gaaaaagagc aggaattgga aacgatccaa acaaactatc gacaacaact aaaacagttg
    70381 gtggaagaaa aagaacaatt tgaaatacaa gaattagaaa aagaaaaaat gaaattgaaa
    70441 gcagaagcag acaaggtgca agcaactttg aaagatcaat atcaaaagca tcagagtcaa
    70501 gcgatcggtg cgatcattga aagggtgaaa gaaacttatg gccgtcacta agatggaaaa
    70561 agtgaccctc atttcagata aaagaaatca ggagaggatt ttacaagcag tacaaggaat
    70621 ccaaaacgtt gaaatccggg atctgtatca aaatagaaca aacaaccagt gggtggagaa
    70681 gtattttcca gataccacga ttattgataa agaagcaaaa ttaagcgctt taggtaatcg
    70741 gttgaaagac atccaagaag ctatccaatt catacaacac cacggcgata gccgtcaaaa
    70801 gaaactccat ttgaaacgtc aagaactttc cttacaagat ttggaagcca attattccga
    70861 agaaaatttc ataaataaat tgaaagaagt ccttgattta aaagaaaagt gggaacaact
    70921 aactgaagaa cgtgatcgtt tagaagaaga ggaagaatgg cttcttcatt ggcaacagct
    70981 ggatattgct ccaagtatgt atgcaagtaa gacgaccatt tttgagatgg ggactgtgag
    71041 tgcagcaaat tttgaaagtt tcaaggaaga actgagtaag cttgatgacg tttatatgga
    71101 agaaatagat tatacgccaa aacaagttta tctttcgtat gttgctttaa aaaaatcagt
    71161 tgctgttata gaagaaattg ctgggaaata cgggttcaat aaagaaacgt atccttatga
    71221 acaaaatcct aaagaaaaac tgcaagagac caagcagcaa ttatccattg ttcacgagca
    71281 gcaaaagaag ataacgacag cgttaggtca gtgcagtggc tatatccaag aatttgaatg
    71341 ggtagaagaa gtcacacttg ccgttgcaga acgagaaaaa ataaaagaac ggtttatcca
    71401 tgcgacttat ctgatcgttt tgcaaggttg ggtagatgca gaagaaaaac agaatttgct
    71461 gcatgtgtta aacgaaaccg tatcagaaga agatatatac gtcagtttcg aaacaccaaa
    71521 ctcagaagag attcagcagg aagtccctac taaattgaaa aaccatccgc ttgtcgaacc
    71581 ttttgaattg ctgactgaaa tgtacagtct gccaaaatat gaagaagtag atccaacacc
    71641 ttggatgaca ccattttatc ttgtcttctt cgggatgatg gtcgcagatg ttggttatgg
    71701 tttgctgatg ctcattggga caatactagc tcaaaaacta cttgttctac cacgcgggat
    71761 gaaacgtttc gtaaaattct ttgagattct ctcgatacca tcgatcatct ggggattgat
    71821 ctacagttca tttttcggac agtcattacc aaaagaactg tttggcatcc gcttgccttt
    71881 cccaatccta tctacgacgg atgacgtcaa tacgattttg attttatccg tgattttcgg
    71941 tctgatacag attttggttg gattatttgt ggcagcaaag gaaaatatca ggagaaaaga
    72001 ttacttgagt gctgtcagtg atggatttgc ctggcaaggg atcctaattg ggatcgttat
    72061 tgcgttggtt ggtgccatgt tgataaaaaa tacagcattt gtttaccttg gcgctgggct
    72121 atcgatcatt gctgccttat gtatcttgat cgttccgatt atccaaacgc cgtctaaagt
    72181 caaaggtgct gccaaaggtg cgtataatct ttacggactg accggctata tcggtgattt
    72241 agtcagttat acacgtttga tggcattggg tatttcaggc ggaagtattg gagcggcatt
    72301 caatatgcta gttgctttca tgccgccagc tgctcgattc agtgtaggga tccttttgat
    72361 catcgcttta cacgcactga atatgttttt gacactgcta agtgcctatg tccatggcgc
    72421 acgattgcag tatgtcgaat tttttgggaa gttttataca ggaggcggac gtgcctttga
    72481 tccgctgaaa acggcggaaa aatacgtcaa catcaatcac aagaaaaaaa acaataaata
    72541 attggaggat ttttatataa tgatggatta cttaattagt caaaatggtg gaatggtatt
    72601 tgcggtatta gcgatggcaa cagcgacgat tttttcaggg atcggttcag ctaaaggtgt
    72661 tggaatgaca ggtgaggcag cagcagcatt gacaacaagt caacccgaaa aattcgggca
    72721 ggctctgatt ctgcaattgc ttccaggtac gcaaggtttg tatggattcg ttattgcctt
    72781 tttgattttt atcaacttag gaaatgacat gtcagttgta caaggactga actttctagg
    72841 tgcttctctg ccgattgctt ttacaggctt gttttcaggt attgcccaag gaaaagtcgc
    72901 agcggcaggt attcaaattt tagcgaaaaa acctgaacat gctacaaaag gaatcatttt
    72961 tgctgcgatg gttgaaacat acgcgatttt aggtttcgtt atttccttcc tattagtgtt
    73021 gaatgcctaa gcataaagga ggaaaagcag atgaacgcca ttgaaaaaat catctctcag
    73081 atgaacgaag cagcagagca ggaaagagcg gcgttagaac aagaagagcg aatgaagatc
    73141 gatcaaaact ttgaacaaaa acgtacgcaa gttgaaacag aacatcaaaa acaaaaagaa
    73201 aaacaaatcg aattactcga aaaaaaatac cgtcagctaa ggaatcgcca gcaagtagaa
    73261 gtacgacaag aaaatttgaa tgcgaagcag gaatttttaa gacgattgtt tgcagatgcg
    73321 gtaactgaaa tggaaaattg ggatgaatct gaacaaatcc agtttatcaa aaatgcattg
    73381 tattcattac ctttgacagg aaaagtagca ttcattgctg gagaaaaatc agcagcctat
    73441 ctttctcaga cgttgttgga tgaatggaac aatgaacttc cctttatgat ggttttaagt
    73501 gatgaaactg ttgctgatca agcgggtttt ctgataaacg atcaaggagt tcagtataat
    73561 tttcttttca gcagccttgt acaagatatc caaggaacga tgagttttga gattgccaat
    73621 caattattcg aataaggagg gatcagtgta tggaatacca cgaactaaat cccttgattc
    73681 gcggaagaga gcttgaactt ttgtccaatg agacatttga acggatgatt caaacaaatt
    73741 ctgtcgaagc acttggtgaa atattgaaat ccacgattta cagtccatat atttatgacg
    73801 gatatgaata tgacttcgaa cagaatcttt taaaggagca gagccgtcta tttcgctggt
    73861 taaaggaatc agcaccggag cctgaaatcg tttggatcta tacgatgcgt tatactttcc
    73921 ataatctgaa ggttttgaca aaagctgaga tgacaaatca aaatctggac catttgtata
    73981 tcgacgatgg attttattca ttagaaacct tgaaggatgc cattcatacg caagtttcaa
    74041 atgaacttcc tgaaagcatt atggacagta tacgagaagt acgtgattat tttgaagagt
    74101 ctaccattct tcagggaatt gacgtgattt atgatcgaca gtttttgaca gagcagcgac
    74161 ggcttgccga taagctagga tatcaagaat tgctgacaga agtcattgct tttattgact
    74221 tgaccaatat cacaacgatg gctcgaggaa tattgcagaa aagaacatat gggttcatga
    74281 cgacggtact ttccagctcg ggaagtatcg aaaaagaagt atttttgaac tttgtagata
    74341 aagatatgga agattacacg aattttctat tagagacaga ttatgctgat tttctgcgcc
    74401 ctgtcatccg tgagggaaat attgatctcg taaagcttga acagttgaaa gatgattatc
    74461 taagttcatt gtatcaagaa gcacaaaccc aggctttcgg accactgcca ctactagcct
    74521 ttttgaacgc aaaagaggta gaaagcaaaa accttcgttt actggttatc ggcaaaagaa
    74581 gccatttcga atcagaaaca atcagaaagc gggtgagaca agtctatgac gcataagatc
    74641 ggtgtagttg gcgacaaaga ttctgttttg ccattcaaat tgtttggctt tgatgttcgt
    74701 tatggtacaa ccaagcaaac cgttcgtcag gcaattgaag aaatggcaaa gaatgagtat
    74761 ggcgtgattt atgtcacaga gcaatgtgca gcattgagcc cagaaacgat cgaacgttac
    74821 aaggaacaaa tgatacctgc tatcgtattg attcccaatc atcaaggatc attagggata
    74881 gggctggaag aaattcaaaa aaacgtagaa aaagcagtag gtcaaaatat cttatagtat
    74941 gaaaaggaga tagaaatttt gcaaattgga aaaatcataa aagtctccgg acctcttgta
    75001 atggctgaga acatgtctga tgccagtatc caagacatgt gtctagtggg agatctagga
    75061 gttatcggag aaattatcga gatgcgtggc gatgtggcgt ctattcaagt atatgaagag
    75121 acttctggaa ttggtccagg cgaaccggtt cgctctactg gtgaagcttt atctgttgaa
    75181 ttaggaccag ggatcatttc acaaatgttt gacggaatcc aacggccatt agataccttt
    75241 atggaaatca cacaaagtaa tttcttaggg cgcggagttc aattgcccgc attggaccat
    75301 gagaaaaaat ggtggttcga accaacagta gaagccggag aaacggtgag tgctggagat
    75361 gttatcggga tcgtagaaga aacaaaagtt ataaaacata aaatcatggt gccaaatggg
    75421 ataaaaggaa cgatcaaaac aatcgaaaaa ggttcattca ctattgatga aacagtatgt
    75481 gtcattgaaa ccgaaaatgg tgacaaaaat ttgacgatgc ttcaaaaatg gccagtaaga
    75541 cgtagtcggc caatcaaaga aaaattaaac ccggatgcac ccatgatcac tggacagcga
    75601 gtgattgata cattcttccc agtgacaaaa ggaggagcag cagctgtacc tggtcctttt
    75661 ggtgcaggga aaacagtcgt tcagcatcaa attgcaaaat gggccgatgt ggacatggtt
    75721 gtctatgtgg gatgtggcga gcgtggaaat gagatgacgg atgttttaaa tgaattccct
    75781 gaattgattg accctaatac aggagaatcg ttgatggaac gtacggtatt gattgctaat
    75841 acatcaaaca tgccagtagc agctcgagaa gcttctatct atacgggaat cacaattgcg
    75901 gaatatttcc gtgacatggg ttatgacgtt gctatcatgg cagactccac ttcacgatgg
    75961 gcagaagcac ttcgtgagat gagcggacgt ttggaagaaa tgcctggtga tgaaggttat
    76021 ccagcttatc ttggttcacg gcttgcggaa tactatgaac gatctggaag agtcattgca
    76081 ttaggatcag aaggtcgcga aggaagtata accgcaatca gtgctgtatc cccgtctgga
    76141 ggggatattt cggaaccagt cacacaaaat acattgcggg tcgtaaaagt attctggggt
    76201 cttgattcca gtttagccca aaaaagacat tttccttcga tcgattggat acaaagctat
    76261 tctctttatt caacagaagt agggaagtat atggatcata tccttcaagc tgactgggca
    76321 tccatggtca ccgagggcat gcgtattttg caggaagaac agcagctgaa tgaaatcgtg
    76381 cgtttggtag ggattgattc cctttcagat aatgaccgtc tgacattaga agtggcaaaa
    76441 tccattcgtg aagattattt gcagcaaaat gcttttgatg atgtcgatac attcacttca
    76501 agagagaaac aattcaaaat gttgaagatg atcttgacct ttggtgaaga aggacgccgt
    76561 gcgttgtcgt taggtgctta tttaagtgag atcatggacg gtaccgtatc gattcgtgaa
    76621 aggatcggac gaagcaaata tatcccggaa acagaattag aaaaactaga tcaaattatt
    76681 ggtgaaatca agcaaacgat gcaaacgatc gtttcagaag gaggcatgag caatgattaa
    76741 agaatatcgt acgatcggtg aagtcgtcgg tccattgatg gcggttgaaa aagttgctgg
    76801 tgtcaaatat gaagaattaa tcgaagtccg tatgcaaaat ggcgagattc gtcgtggaca
    76861 agtattggaa gtccaagaag ataaagcaat ggtccaaata ttcgaaggaa caagtggtat
    76921 caatttacgt gactcttctg tacgattttt aggccatcct ttagaactag gtgtatcgga
    76981 agacatgatc ggtcgagtat tcgatggttt gggacgaccg aaagataacg gaccagagat
    77041 cctgcctgaa aagcatttgg atattaacgg agaagtaatc aatccagtag ctcgggatta
    77101 tccagatgag tttatccaaa caggtatctc tgcaatcgat catttgaata cattagttcg
    77161 tgggcaaaaa ttaccagtat tttctggctc tggtcttcct cataaagaat tagctgctca
    77221 gatcgcgcga caagcaacgg tactcgattc atcagatgat ttcgccgtag tctttgctgc
    77281 tattggaatc acttttgaag aagctgaatt tttcatggaa gattttcgtc aaacgggcgc
    77341 aatcgaccgt tctgtcatgt ttatgaattt agcaaacgac cctgcaatcg aacgtatcgc
    77401 tactcctcgt atggctttga ctgctgcaga atatcttgct tatgaaaagg gaatgcatgt
    77461 tttggttatc atgacagaca tgaccaacta tgcagaggca ttgcgagaga tttctgcagc
    77521 acggcgagaa gttccaggaa gaagaggata tcctgggtat ctttacacga acttagctac
    77581 tttattcgaa cgagcaggaa gaatccgcgg actaaaagga tcggttactc aaattccgat
    77641 tctaacaatg ccagaagacg ataaaactca tccaatccct gacttgactg gttatattac
    77701 agaaggacag atcatcttga ctagagagct ttacaagagt gggattcagc caccaattga
    77761 tgtactcccg tctctttcgc ggttaaaaga taagggaact ggacccggaa agacaagaga
    77821 agaccatgca gctacgatga atcagctttt tgcagcctat gcacaaggaa aacaggcaaa
    77881 agaattagcc gtagttttag gtgaatctgc tttatctgat gtagacaaga tctatgcgaa
    77941 gtttgctgag cggtttgaaa aagaatatgt caatcaagga ttctacacga accgttcgat
    78001 tactgaaaca cttgatctag ggtgggaact gctatcgatg ttgccaagaa ccgaattaaa
    78061 acgaatcaaa gacgacatgc tggacaaata tctgccagaa gggaagtgag ttctgatggc
    78121 acgattgaat gtgaatccga cccgcatgga attgactcga ttgaaaaaac agttgacaac
    78181 tgccacaaga gggcataaat tattaaaaga taagcaagat gaactgatgc gtcaattcat
    78241 cctgttgatt cgaaagaaca accagctgcg tcaagaaatg gaagagtcta tgacagaagc
    78301 aatgtctgat tttgtattgg caaatgcttc gatcacggaa cctttcatcg aagaattatt
    78361 tgttttgccg gcggaaaacg ttgatttgtc tatcgtagac aaaaatatca tgagtgtaaa
    78421 agtacctgtg atgaattttg aatatgatga acagctgaca gacacacctt tggaatacgg
    78481 gtatttgaat tccagtgccg aactggatcg gtcaatcgat cggtttacta ccctgcttcc
    78541 aaaacttttg gaactgacag aaatcgaaaa aacttgtcaa ctgatggcga gtgaaatcga
    78601 aaagacacgt cggcgagtca atgcattaga atatatgacg atccctcaat tagaagaaac
    78661 gatctattat attcgtatga agctggaaga aaacgagcgc gcagaagtaa caaggttgat
    78721 caaagtaaaa aatatgggta ccactgacta agtggtatct ttttttaaaa gaggaaaacg
    78781 atgattaaac aaagcaaaca aataaagaaa cgtttgccgg ctgttcagct tctcgcatta
    78841 ggattttttc tcctgatttt agttggcggc ggcctactta ccctaccatt ttttagtaat
    78901 agtgggcatg ggacaaattt tatcgatgcg cttttcactg caacttctgc tgtctgtgta
    78961 acgggattga caacattgaa tacagcagag cattggaaca gtgctggtca atttttgatc
    79021 atgcttttga ttgagatcgg cggtcttggt tttatgatgg ttccgattct atgctttgca
    79081 ttgataaaga agaaagtcag tctcagcaca cgtattatat taaaagaagc gctgaatcta
    79141 gaagaagtat caggtgtgat gaagctcatg ctttacatcc taaaattggc ctttgtgatc
    79201 caattgattg gtacggttgc tttatctttt gtatttgtgc ctgaatttgg ctggttgaaa
    79261 ggtatctggt acagtttgtt ccatgcaatc tctagttttt gtaatgcagg gtttgatttg
    79321 ttaggagaca gtttagccaa tcaccagcaa aatgtttatt tgatcatggt ggtatctgct
    79381 ttgatcattt ctggtgggtt aggcttcatc gtatggcgtg atttactgga atatcatcag
    79441 aaaaagaaga tgacgattca ttcaaaaatc gcattaaccg ttacaggtac ccttttatta
    79501 ggcggattcg ttgtgttctt tttcacagag cgcaatgggg caacgctagt ggaagggaat
    79561 atgtttcaac gattggcaaa cacctttttt atgagtgtga cacctagaac ggctggatat
    79621 tattcagttg attacctcca tatgagccat gcaggtctga ttttaacgat gtttttgatg
    79681 tacatcggcg gaacatctgg gtcgacggcc ggcggattga aaacaactac gttaggtgtg
    79741 ttgctgatcc agatgcgttc gatgttgaaa ggacgtacga gagcggaagc atttggccgt
    79801 accatccgac aaggcgcagt gttgcgggca cttactttat tttttgttac gttgacgttg
    79861 tgtgtagtcg ccatcatgat tttatcggtg actgaaacga tacctaaaac ttctggcatc
    79921 gaatatattg cttttgaggt cttctctgcg tttggtacag taggtcttac gatgggattg
    79981 acgccagact tgactgcaat agggaaattg atcattatag cgcttatgta cattggacga
    80041 gttggtgtat tgactgtggt cttctctctg atgacaaaag cacaaagaac acaatcaaaa
    80101 tacaaatatc cagaagaaag tgtcatgatt ggataatttc atcttctgac agaaacctag
    80161 tgatttagta aaataagaaa ataaaataat ttttcttgct tcttggatac tttaagtgca
    80221 aaatgttcgt ttattttttg aggcactacc cgttgttgaa cctcgggtag tgcctctttt
    80281 ttattgaaag gaatcaataa gatttgattc ctttatttat caataggtta taattcatat
    80341 ttttttattt tttcttttac gatacttagt ccagaagcta aaatttcaag gttattagag
    80401 gatagggaaa ttcgaacgaa tgcatctggt tgcgtacgtg tagtaaagcg tctggaatga
    80461 taaagtcgga taccgttagc aagaaagtca ttctcaatta tcttagggtc aatctcagga
    80521 tcaactggaa gaattcgata gtaagggaca cgattcgttg gtttttctac tttgaaaaga
    80581 gaatcaaaaa gctgattggc tctttttgta agacttaatt tagtttctaa gatcctattt
    80641 gcttcatttg aatggaggat ttgagtgatg acttccacat ctaatccaga agttttcaca
    80701 tttatattaa aaattgcttg ttctatcttg ctttggacat gtactggaac tacaagataa
    80761 gcaactcgca atccagaaca aataaatttt gtcattccag ctaagtagat tgtttggtta
    80821 ggtaataaat tttggaaagg aggaatgatc gtatgttgat agtaagttgt taaaaatgaa
    80881 tggatatcat cttcaataac ccatagacct tctttttcaa tcacttcggc taattgctct
    80941 cgtcttttgt gagagatttg aaaccctata ggattattac aagaaggcat aagaaagaca
    81001 ccatgaatct tcttttttct acattcctga atcaatatat caggtcgcat tccttgctcg
    81061 tcaaaatcaa ttggaacgat ctctaaatga tataactgag ccaattcaat gaaattagcg
    81121 tatgtatatc ggtctgttgc tattctatca cctggagaga aaagtgcaga aagtgtgata
    81181 gccaatccat tttgaacacc ggaaacaata gatagagatt ctaaggacgg aatgactcct
    81241 tgagttttta accaatttgc tgcagtagtc aattgaaaat ttgtgccttt gggatcttgg
    81301 taattaagca aaccaacaag atttgggtta ttactagttg attgaataaa cgggacaacg
    81361 aatttattac attcttcaaa agaactaacc agaccaaaat caatgatatg ttcggttact
    81421 tcattggtag agatggtgat ggactcaaag acattgggag aaataaatgt gccacttcca
    81481 atattcgtat acagcaaacc tttctcaatt ccatatttat aagccttgcc aacggtagta
    81541 aaatttaaat ctaaataatc agctagctct ctttgggagg gtaactgagt gtttttctgt
    81601 aatttattct ttaaaatatc ttgttctaat tgttgaatca acgatcgata ataaggacgc
    81661 ttcaattttt gtttgtctgg tcgccaagtt aacgggtaat tgtcataaga attaataggc
    81721 attttctcca ctccttaaaa ttgtatacca tacaatttta ccattgattg tattgatttt
    81781 agaagttaat cttttgtgag gagggaattg tctatgaaaa ttattaattt tgaggaagta
    81841 gtagcttctt ttagttttga agaagcgatt caagtaatga aaaaatgttt ttttgattat
    81901 gaaagtggta aaatcagcca aaatcctcga ttggtggaaa ttttacctga cggaaaaggc
    81961 aaaaatatgt ttgcgatgat gcctgcttat ttgggggaga acagatattt tggcacaaaa
    82021 gtcattaccg cttttccgga taatcatcag tatcatttac cttctcattt aggagagatt
    82081 ttcttgtttg atgctcaaaa cggactaccc aaagcattga taaacgcaaa tgcagtcacc
    82141 tggattcgaa cagctgctgt atctgcgctg gcaacagatt acttagcaaa tccgcatgca
    82201 gaaacacttg ctttaattgg agctggtcaa caagcattgt cgcatttgac tgcgatttct
    82261 accattcgtc ccatcactac agtgtttgtt tacgatattt tagaagaaag acgaaatact
    82321 tttatcgcta aagctaaaaa acagtatcct aaaataacgt ttataaatgc aatatcagta
    82381 gaagatgcag tacgtccagc ggaaatcatt tgtacgttga cctctagtaa agaggcattt
    82441 cttcaaaaag agtgggtaag ggaagacgca catatcaatg ctgtgggcac atttactcct
    82501 gatacaagag agataagtac atcgttgatg gaaacttctc aaatctatgt agatgattac
    82561 tcagctgcct taacagaaag cggggacctg cttatagcca tggcagaagg agatttagag
    82621 gaatcttgta ttgttggtac attaggcgaa ttagtttctg agaaaaaaac attttataaa
    82681 ggagaatcga ggccgacgat tttcgacgca gttggtctag ctattgaaga tctttgttgt
    82741 gcagaatata tttataacaa attagagaaa ggggctttaa aatgaactta gattattacg
    82801 tagttgatgc atttgcagat gaggtgttca aaggaaatcc agcagcggtt tatgtgctag
    82861 aagaatggct gccagaaggt acgatgcaaa aaattgccat tgaaaacaat ttatctgaaa
    82921 cagcatttac agtgaagaaa aaccaagaat ttgagcttag atggtttacg ccagatcgag
    82981 aaatcgatct ttgtggtcac gcgacattag caacagcatt tgttttattt aactattaca
    83041 aaatacctga tgaaacgatt aaattttcta ctcaaagcgg taatctattt gtgacaagaa
    83101 ttaaagatta ttattacatg gattttccta gtattatgcc gaaaaaagtg ccgatattgt
    83161 ctgaatatga agaagcgatt ggggctaaga ttaaagaagc ctacttagca cgtgatttat
    83221 ttttcgtatt agaagatgaa aaaacggtgg ctaaattgca gccagatttt acagctatca
    83281 aaaattttga attaggaata ggagtcatcg tgacagcaga aagtgtgcaa aaagactttg
    83341 tttctagaac cttctttcca aaattaacaa tcaatgaaga tccagtatgt ggatcagcac
    83401 attctaatct tattccttat tgggcagaaa gaatgaataa aacaaaattg tcagcttatc
    83461 aaatgtcatc aaggggcggg gatctagaat gtgaattaaa agaagatcgt gtgataatag
    83521 gaggaaaagc agttttgttt tctaaaggta cctcgtttat ttgatttctt tcaaatgaat
    83581 taaaaataaa ttggtggaga gtggcaacct tatagctaca atggttgtta aaaaaagacg
    83641 caaacttttt caatttaatg aaaagtttgc gtctcatttt aattattggt gtttgttttt
    83701 tattcaatca agagtaaaat gccccatact aactatcctc ggatgacttc gattttataa
    83761 ccatcagggt caatgacaaa gtagtaagaa ggcgcagtgc caggtaatcc ttttaagtca
    83821 gtaatctcaa gaccagcagc tttatgttgc tcgtgtaatt tttctagatc atcagctgct
    83881 atagcgatat gaccatagcc gtctcctaga tcatacgcac catgatcata gttgtaagtc
    83941 aactctaatt catagtcatc cccaggaagt gtcaaataaa caagtgtaaa tttgttttct
    84001 ggaaaatcac ggcggcggct ttcttcaaaa ccaaatgcct tttgataaaa ttcgattgat
    84061 gcttcaagat ctttcactcg aacacatgtg tgagccattt tcatagattc atctctcctt
    84121 ttccttttac tcaatcataa caaaaagaag taaggaaata gttgatttgt gcttgttcat
    84181 gagaaatttc tcatgtaaaa tggatagaaa ataaaactgt taaggtggaa actattcatg
    84241 gataaaccaa tttttggtga aaaaaagcaa tttgctgatt atcaaacccg ttatgcagca
    84301 tatattgtca tagaaagaga tcagcagatt gcagtaatcg aggctccaaa tggtgctttt
    84361 tttcttcctg gcggggaaat tgaaggagaa gagacgaaag aagaagcgat tgtccgtgag
    84421 ctgatagaag aaatgggaat tgtggcagaa atttcttctt atctgggaga agctgatgag
    84481 tatttttact cgaattatcg tgcaacttat tattataatc caggatattt ctttgttgct
    84541 gattcttgga agcgtatagg agaaccaact gaaaagacca ataaaatttg gtgggtgagc
    84601 cctcaagaag cgcttgtgaa attgaaacgt ggtagccacc gatgggcagt cgagagatgg
    84661 atcgaaaaaa ataaaaactg aaagaaagcg agtagatcag ctttctctca gtttttttat
    84721 ttattacagt acatattttt tgattgcttc agctacaccg tgctcgttgt ttgatgaagt
    84781 gatgacattt gctgcttctt tgacaagaga aacggcattt cccattgcta ctcctagacc
    84841 agcatattca atcatggata agtcgttctc gttatctccg atagccatga tttcatcttg
    84901 atcaatacca aggtgctgtg cgagattcgc aactgccgca cctttacttg cttctttatt
    84961 tagtacttcg aagtaatagg gcgcactttt gaccgttgtg tatttatcac ggaaagtttg
    85021 cggtaaacgc gcaattgctg catctaaaat ttctggttcg tcgatcatca tcattttgac
    85081 gatgttcatg tctggtgtca tttcttcagg tgttcgatat tttagcggca tcttgactaa
    85141 acttgcttcg tggatcgtat aagcgctgat atctcgatta ggcgtgtaga tcgctttgtc
    85201 atcgatcgaa tggagatgac tgccgacacg gcgtgccatc acttcgatct ctaggaaatc
    85261 ttcatgtgtc agtccgtgac gggcgatgat tttacctgat ttcgtttgtt gtacgagtgc
    85321 cccgttataa gtaataacgt aatcattatc ttgaaaaagg ttcaactcgt ttaactgttc
    85381 ctggacacct ggtaagggac ggccagtgca taaaacgatc gaaacacctt ttgcaacagc
    85441 agcggcaatc gcttctttta cttcttttgt tacttctctt ttctcattta ataaagtacc
    85501 atcgatgtcg atagcgacta gtttgataga cataatttcc tccaatatat tttaatttat
    85561 ttcaatcaat gcgccattac ggatatggct ggcaaattct tgataagttt cgtcaaataa
    85621 atcataatga tcacgcaagg acggatcaac catttctttt ggaaagaaaa atcgctcatc
    85681 tccttgttct tgtcccgcta atgcagcaac aagctgacta acctgtgata attctatcaa
    85741 agttccgtca tttcgcaaaa tctcgatttg tgtgcgatgt ctgccttgtt ctgggcgata
    85801 aaaatcataa ggtagatcgt agctagaatt cacagcagtg taataaacag gattatatcc
    85861 gaccttttct actaacaaag tgagttcttg gactaatttt gaatcccggt tgccagaaaa
    85921 agtagcagat ttcagtggtt tacgattcaa aaaacgttta gctaaatcat ttaaaatagg
    85981 atcagcagat tcagaccatt gagtaaagta agtgcccaag accccatcat caagtttgag
    86041 gtattcttct aaagtaaagc ttccgtcaag aaatggcaga agtaattgag aatggagttc
    86101 aaaactgctg gcatcttctt gatacaactc gtgtgcgcga tgaagcagat gctccaaaat
    86161 tacttccata ccacgggaaa caggatggaa gtacacttgc acatacattt gatatcggct
    86221 aacgatataa tcttccactg catgcatgcc actcatggaa aaagcgatcc cgccttcata
    86281 aggacgaatc acacgtaata ttcgggtaag atcaaatgtt ccatattctg ttccagtgaa
    86341 ataagcatcc cgaagcaaat agtccattcg atctgcatcg atctgactag aaatcatttg
    86401 gacaacttgt gggttggggt aagttttttg gatcacacta gctacctttt caggaaaacc
    86461 ttcttctact cggttaagaa tctgatacac ttctgtttct ggagaagtga tgatctctac
    86521 agtgatggct tcatgattcg tatggaagat atgttcaaat gtatgagagt aaggaccatg
    86581 accgacatca tgcaacagtg ccgcacataa agtcaccagc cgttcctgat catcccagcc
    86641 tccgttgcct attttttctt ttgaaaaatt tcgagaaaag atatcgcaga ttcgtcttga
    86701 aatctcataa acgcctaatg aatgagtaaa acggctatgt tctgctccgt gaaaagtgaa
    86761 agaagaagtg cctaattgtt taatgcgtcg tagacgttgg acttctttcg aattgatcaa
    86821 atctaagatt acttgatgct ggacatggat atagttatgg actggatcac gaaaaacttt
    86881 ttccataggc agcatttgat atttataagg ggttgtcata atgtagctcc tttatgttat
    86941 tacgttgttc cattctctgg atttggttgt tccattgctc cagctggatt tcttttttta
    87001 gccagtcggt agaatcctgt aaatcacttg cagaatacag cttttcaaaa gcttttgcaa
    87061 atgcttcttt gacttctttt acaaaaaggg gttggtcaag cagttcttca agtgttgcca
    87121 ttgtagcagg atccactgca ggatagccat ttgtaccaaa gtcttctttt aaagcagatt
    87181 gataaaatcg cttcatgact tgcccgcgtt tttcctgatt accgttgaca cttaagtaca
    87241 tcataacaga aatcccattt ttgatccgac gctgagcgat accggcaaat tttttgccat
    87301 ggatgcttaa atcataagtt ccaggacaat aagaatgggt gatttcgaat gcttgtatcg
    87361 gtgcctttgg aaaagcgtat ttagtcaata ggagcatttg ctcgtaagcg tcatctatgg
    87421 ttattttttt tgtgtcactt tgggggaaaa tcaaagaaat attcaaaatt ccttcatctg
    87481 atacgatccc taatccacct gaatttctga tcactggagc gtaacctgca tctttcagtg
    87541 tttggatccc gtcacttaga aaaggagttc gaacatcctt catacctaag atgactgtgc
    87601 agtcatattg ccagaaatgt aaaaagattt gctggtgttc tttagaatat tccgttaata
    87661 tatctgttaa tgcaaaaggg gaaaagtcgt ttttttgata aatgtgctga tctagtcgaa
    87721 tcgcagggtt cattcaaccg gctcctttgt ataaattcat attttctaca atattatact
    87781 ctaaaatcaa agagttgttt tggaaaaaag gttttctgta aaaatggaca agttggttga
    87841 caaaacaaaa gaaactagga acaatgtagc tatgagaagt aaaggagaat gaataatgga
    87901 attatcacaa ggaacaccga tttcaatcaa attgcgtaca aaagtaaaac agcaagggga
    87961 agtgcaagat ttttattttg atttgaaagg gcaaatggtg acaattggcg atacgctgta
    88021 catacgttat aaagagattc aggaggagac gggcgatgag attcctgtaa cgatcaaatt
    88081 agttccagat ggtcatgtac agctgatccg tgcaggagaa atgcggatgc gtttgaaatt
    88141 tggctataaa gaacggttag aaacatccta tcgaacacca tacggtatga tccagattgc
    88201 tacttttacg aaagaactgc atgttagttt gaaagaccgt cccacagcag ggaaggttcg
    88261 aatcgattat gatctgttca tgggaccgga aaaaatcggc gaatattatt tgaccttgga
    88321 ttttacagcg taaatataaa agaaaagttt gattttttgt gtctgatttt gtatgataag
    88381 gagtagactg tgaaaggacg tgtcctgatt ggaaattagt gtatttgaag gtgcaaacaa
    88441 aagcgaatta tcaatgatcg aagtagcaca cgcgatctta gaacaacgtg gcgatgtcat
    88501 ggatttttct gatttagcta accagatcca aaactatctt gggaaatcag acagtgaaat
    88561 tcgtgattca ctcgcacaat tttatactga cttgaatatt gacggcagct ttatctcatt
    88621 aggggataat cgttggggct tgcgttcatg gtatgctatc gattcgatcg atgaagaagt
    88681 aaaccatgga atggacgaag aggatgaaga tactccacgt cgccgtaaac gcaagaaagt
    88741 caacgccttt atcaatgatg atgaagacgc gatcgattac aatgacgatg acccagaaga
    88801 tacagatctg acagaagaag atgacgatga tctgtttgat gacgatgatg atgaagatga
    88861 agaaatcgct gcttataatt ctgacttgca agaaatcgga gcagatgacc cgacagatga
    88921 tgaagaagac ttgccaggaa atatcgaaga agatttaagt atcatagacg aagacgatga
    88981 tgatgaagat tatgaagagg aagaattttc tgactctgaa aaagaataaa cattattttc
    89041 ttacgagagg ttgtgacaga gacgatcagc ctatcagaat aaggaggtgt gacacgattt
    89101 ttgtcacacc ttcttttttg tatataaaaa tcccaagtaa atacataaaa aataaaaaaa
    89161 ttgaaacttt tagcgcacct tgtacgtcat attaagtgag gcagaaagga gtggagaatg
    89221 atttttactg gatataagaa aaaacggcag atcgaaaaat accgtcacct gctgaatgga
    89281 ctcatcgaaa ctgattatca aaaaatgttc cgtatcacga tgaattacgt acatgataaa
    89341 gaagaggcct tagatgtgat gcaagatagt tttcataaag ccctgaacta ttttgaaaaa
    89401 gaaaaagaga tcgagcattt tagtgcatgg ttttatcgga tattgatccg cacggcatta
    89461 gatagttggc gcagacaaaa aaggacgcct gtggacttat ttgaaattca agatatcccc
    89521 caacagttac ctttagaaac ttccgttgca gagctccata tgattttaga taaaatagac
    89581 agtccggaaa aagagattct gatcctctac ttttttgaag gatttcgttt gaaagaaatt
    89641 gcgactatct tggatatgaa tgaaaacaca gttaaaacga aaatgtaccg ctcattgaat
    89701 gctttacgtc aaatcctaga atgaaggaga gaagagtgcc atgtattccc aaaaagaaaa
    89761 tttaaaaaaa ctaaagaaaa cttataaaga acaagcaatt cccaaagggc ttaaagagga
    89821 gatccgccag cgttttatta tcgaagagca atcatttata aaagcaaagc ggagaaagag
    89881 acgtctccaa gggttcggag tatctttggc tatcgtaatg attacaacaa gtagccttct
    89941 ttttaatagt caagttcgaa gctttgcaga agatttaccc atcttaggat cagtcattga
    90001 attgatctta ggcgaacgct ttactgatcg atctgaaaaa atcgatattc aggtaccgaa
    90061 gatcaataca caaaatgaga aagaaaataa aacaattcat ggattaaatc aaaaatactt
    90121 tcgcgaagga caagcagatt ttgaaaaagc tgaaaaagaa tatggaaatt ttgaaacgga
    90181 tcattaccaa gttttgggag attatcagaa aatcgtagac gataaccggt ttctcgtgat
    90241 tgaaaggcag atcacccaaa cagcagcaga tagccatgtt gaaaaaagat acgacacaat
    90301 cgacaagaaa aacagtgtcc agctctcact tccactttta ttcaaagatg acacctatct
    90361 atctgttttg acaaaagaag tcaaacggca gatggccgaa caagtcaagg aagatccatc
    90421 taagtactat tggactgagc aagatattca agaagggacg atagaaaaac caacacttgt
    90481 tacgcctaca cgaagttttt atttgaacaa agagcatcaa ttagttttga ctttttctca
    90541 atacgaaatt gctcctggtt atatgggtac accagaattt gtcattccta agtccgtgac
    90601 aaaaacgatt ttggcatcgg aagattattt ggatcattag acgagaagta gttgcgacta
    90661 gcagtttcaa ttttcaagta agtacaagta aataataaga agagaaaaag agaaagaaga
    90721 gatatagtga aaagaaatta tttagttatc agtattattg ctttatccgt gcttttgttt
    90781 ttcgaaatta cttcggtggc ctccttgatc accgaagtaa aaggacaaac acactcttcg
    90841 gagcattcgt cacaatcttt caaacaatca gaacaatcaa ccattaaaag ccaatcaact
    90901 gaaacagcta acgtcaaaga agccattcaa gaatcagcgg aaaaggtaca aagtcagtca
    90961 tcacaatcaa cacaagacag tacaaaaacg agccgagatc cggctgtcaa aagcgtcgca
    91021 atcagctttg atgatggacc aggagcgacc acaacaccgc agcttctgcg aatcttaaaa
    91081 gaaaaaaacg tgcatgccac ttttttcgtc ttaggagaaa atacggcgca gcatcctgaa
    91141 atcgtgaagc agacagctga agcagggcac gagattggca atcacacata cgatcatcag
    91201 aatctggcca ctctttctgc ccagtcgatg acggaggaag tgacgaaagc agacactgag
    91261 atcaaaaaag tcactggcaa aacccccact tttgttcgtc cgccctatgg ttctgtcacg
    91321 aatgtaggag caacgatcat ccagcgaccg atcatcgaat ggtctgttga ttcagaagat
    91381 tggaagacaa gaaatcctga tttgattttg cagaagatcc aagcaacagt atacgatggg
    91441 gcaattattt tattccatga tatctatcct gaaacgattc gtgccgttcc tcaagtgatc
    91501 gattatctga aagagcaagg gtatcgcatc acgacagttg gggatttatt agggcatcca
    91561 acggctgtcg agaactatta tggcagaaac gatcatcggc cagttcagta agtgagacaa
    91621 gtatctttaa taaataacac attttaataa gtagttgaac acataactac cttacttgca
    91681 aaggcttaac aatatctata caggtacaac aaaaaactgt ccaccaattt tctttggcgg
    91741 acagttttca cttaaaaata aagaagcaac tattcgtaaa ttatgctaaa atagcagtag
    91801 aacgcgtcct gagggaatcg aacccccgtc tcaagaaccg gaatcttacg tgatatccac
    91861 tacactaagg acgcaagcac tgggataatt gtaactaaac agataaaaaa ttacaagggg
    91921 attggacaaa aaaatgaaat ttgagcaaaa tttttcacaa aaacagcagc agacacagaa
    91981 actagccatg acgcaacagt tgcaacagtc catccaagtt ttacaatata acagtgagga
    92041 gctgttggca ttcgttgaga atcaagcaat ggaaaatcca ttggtggaag ttgttgagcc
    92101 ggaatggcag ccagattaca taaaagcttc ttcatcttct tatgaaggag aagagacaaa
    92161 ttacctaaat cagattcctg atacaaaagg gtctttattt gattcattga tcgaacaagt
    92221 ccatttgaat taccgagata cttttttgag gaaaatcgtt ctttatcttg tagaatacat
    92281 cgatttgaac ggatttttga cgattagttt agaagaagcc ggaaaagaga ttggagctac
    92341 gcctatccaa atgctcgatg cgttgactct gattcagcaa ttagaaccag ctggagtagg
    92401 tgcacgtagt ttgcaagaat gtctcatgct tcaaacagaa cgagatgatt atgcgccgga
    92461 attagcttat atcgtgttgg aagaatgttt tgaggaactg gtggagcgaa aatggaaaga
    92521 aatcgcacaa cgtttttctg ttgatctaca tgctgttcag cagatattcg actatttaca
    92581 aacgttgagt ccaagccctg gccggatttt cgaccgtagc agcgaattgt acattattcc
    92641 tgatgtccgg gtattagtgg acgacgataa aaatgtacaa gttatctcaa accgaaaaaa
    92701 tcagccaaat atacgctttc aagagagtta cttcaaacaa atgtcacaac aagcagataa
    92761 agaaactgaa aattatttaa aagaacggaa acaggaattc gaatggttga agaaaacgat
    92821 cctacaacgt ggggatacaa ttttgcgggt tgctcaagtc attgtttccc ggcaaaaagc
    92881 tttctttatc gacaaggaac gtccaatcaa gcctcttacg ttaaaggaag ttgcaactga
    92941 gatagatgtg catgaatcca cggtcagtcg tgcagtcaac gggaaatatt tggaaacaga
    93001 cttcggtgta tttgaattaa aaaaattttt cactacacga attcctacga atagtacaga
    93061 acagacagaa gacctttcag cagatacagc gaagaaaaag ctgcaggaat tagtcgatca
    93121 agaagacaaa aacaagccgt tgtctgacca gaaattggtt gaattgctga aaaaagatga
    93181 gatagcgatc tcaagacgaa cagtagcaaa atatcgtgat ttattaggaa tcccgagttc
    93241 atcaaaaaga aaacgttatg ataactaatc agtcaaaaga ctagaaaaag tatttcactt
    93301 tttctagtct ttttagttga aaaacgagag caaaactgtt agacttacag tcgtgaggtt
    93361 gagatttccc tttattgatg tgaaattcga atagttaatt tcttttttta tcgtatatgg
    93421 gtcgttttaa gtctacattg gacgtaaagt gtccaactaa ggagatcgct atgctggatg
    93481 aaataaaaat gattgaatct gttgcaccag atattatcga agtgttgcaa gaacgctata
    93541 aaattttacg aaatatctat tggatgcagc cgattggacg tcgaagttta tcggaaagta
    93601 tggggatcac agaacgagta ttgcgaacag aaacagatct tttgaaaaat ctgaatttga
    93661 tcgacacctc aaaaagcggt atgacgctga caactaaggg cgaagaagtt tatcaaagcc
    93721 ttgaaaactt catggatcag cttttaggta cgcatcaaac cgaacaacag ttggcagaat
    93781 attttggtaa ccaacgctgt atcgttgttt caggaaacag cgaagagcag accaaagtgg
    93841 ctgatgcatt cggcgaagcg ctgagcgaag ccctagaccg actacttcca gaaggcgaga
    93901 atatcatcgc tgtgatggga ggaactacta tggctgtggt agccgaacaa ttgtctaatc
    93961 tggaaaataa gaaacggcat aacctgtttg tccctgcaag aggcgggatt ggtgaagcga
    94021 taactgtaca ggcaaattct gtcagtgcaa gaatggctgc taaagcaaac ggtaaccacc
    94081 gtgcacttta tgttccggaa caattaagtc tggcaactta caattcttta ctcaatgagc
    94141 catctatcca agaagtctta aacttgatta gtgaagcgaa ttgtgttatt catagtattg
    94201 ggcgtgcatt acacatggca gcacgtcgta agatgactga aaaagaatta gtcatgctga
    94261 aacaagcaaa tgctgttgcg gaatcttttg gctacttttt caatgaaaaa ggtgaagtcg
    94321 tttataaggt ccctagaatc ggcatagaat tgcaagattt ggaaaaagtc ccgatcatca
    94381 tggcaatcgc gggcggaaaa tcaaaagcta aagcaattcg agcttatatg aaaaatgcac
    94441 caaaacaaac gtggctcatc actgatgaag ctgctgcaaa tgagatttta aaaggggtaa
    94501 ccctttaaaa taaatatttt tttgattttc ataaggagga aatcttaaat gacagttaaa
    94561 gtaggtatta atggttttgg acgtattgga cgtctagcat tccgtcgtat ccaagatgta
    94621 gatggaatcg aagtagtagc aatcaacgac ttgacagatg ctaaaatgtt ggcacacttg
    94681 ttaaaatacg acacaactca aggacgtttc aacggaacag ttgaagttca tgaaggttca
    94741 ttcaacgtaa acggaaaaga agttaaagta ttagctaacc gcaacccaga agaattacca
    94801 tggggcgaac taggcgtaga tatcgttcta gaatgtactg gtttcttcac ttctaaatca
    94861 gcagctgaaa aacatttaac agctggtgct aaacgcgttg ttatctctgc tcctggcgga
    94921 aacgatgttc caacaatcgt ttataacaca aaccacgaaa cattaactgg taaagaaaca
    94981 gttatctcag gtgcttcatg tactactaac tgtttagctc ctatggctaa aacattgaac
    95041 gacaaatttg gtgttgttga aggattaatg acaactatcc acgcttacac aggtgaccaa
    95101 atgactctag acggacctca tcctaaaggt gacttccgcc gtgcacgcgc tgctgctgca
    95161 aacatcgttc ctaactcaac aggtgctgct aaagctatcg gtttagtaat tcctgaattg
    95221 aacggtaaat tagacggagc tgctcaacgt gttcctgtac caacaggttc attaacagaa
    95281 ttagtaacag ttcttgaaaa agaagtaact gttgacgaaa tcaatgcagc aatgaaagaa
    95341 gcttcaaacg aatcttacgg atacaacaca gacgaaatcg tttcttcaga tatcgttggt
    95401 atgacttacg gttcattatt tgatgctaca caaactaaag taatgacagt tggcgacaaa
    95461 caattagtta aaactgttgc ttggtacgac aacgaaatgt catacactgc acaattagta
    95521 cgtactttag aatacttcgc taacttataa gatagacaaa cgtacaagtg taaattatag
    95581 acatgtacat ttaaaagcgg ggaagcaatc gcgcttcttc cgcttttttt gataataagt
    95641 ttattcagga ggaaataaaa tggctaagaa aacagtgaaa gatatcgatt taaaagacaa
    95701 aaaagttctt gtccgtgttg acttcaacgt tcctttgaaa gacggcgtga tcactgacga
    95761 cactcgtatc aaagcagcat tgccaacaat caactacgtt ctagaacaag gcggaaaagc
    95821 aatccttttc tctcaccttg gacgtgtaaa aacagaagaa gataaagaag gaaaatcttt
    95881 agctccagtt gcaaaacgtc taggcgaatt attaggtaaa gaagtaacat tcgtacctga
    95941 aactcgcgga gaacaattag aagaagcaat ccgcaacatg aatgacggcg acgttgtcgt
    96001 attcgaaaat acacgatttg aagatattga cggtaaaaaa gaaagcaaaa atgatacaga
    96061 actaggtaaa tactgggctt cattaggcga tgtatttgtt aacgatgctt tcggtacagc
    96121 tcaccgtgca catgcttcta acgtaggtat cgcttcaact ggtatcccaa cagttgctgg
    96181 attcttgatg gaaaaagaaa tcaagttcat cggtgaagca gtagaagaac caaaacgacc
    96241 aatgattgca atccttggtg gcgcaaaagt ttctgataaa atcggcgtaa tcgaaaactt
    96301 gattcctaaa gcagataaaa tcttgatcgg tggcggaatg acttacacat tctacgaagc
    96361 aaaaggaatc aagatcggta actctctagt agaagctgat aaagtggaac tagcaaaaga
    96421 attaatcgaa aaagcaggcg acaaacttgt cttgccaatc gacaatgtct gtgcaccaga
    96481 attttcaaat gatgttgaaa cacaagtaat cgaaggagac attcctgatg gtttgatggc
    96541 tttagacatc ggcccagcat cagtaaaatt atttgctgat acattacaag gtgcaaaaac
    96601 agttgtttgg aacggaccta tgggtgtatt tgaaatgagt aactttgcta aaggtacaat
    96661 cggtgtttgt gaagcaatcg ctaacttaga aggcgcaaca acaatcatcg gtggcggaga
    96721 ctcagccgca gcagcagaac aattaggatt tgctgacaaa ttcacacaca tctcaacagg
    96781 tggcggcgca agcttggaat tgcttgaagg taaagaatta ccaggactag cagcaatcaa
    96841 cgataaataa acgaccaagt ttgctcaatt ctgacgagta atcgaacaaa aggtgaaggg
    96901 agttttcatg actccctagc catttttcaa taaattaagg agtgtgttcc tcaatgcgta
    96961 aacctattat cgcaggtaac tggaaaatga acaaaactgc atcagaagca aaagaatttg
    97021 ctgaagcagt aaaaaacaaa atcccttcaa atgacaaagt tgattcagtc atcggatctc
    97081 ctgcattatt cttacaagaa ttaatgacat cagctgaagg tacagaacta aaaatctcag
    97141 cacaaaactg ctactgggaa aactcaggtg cgttcactgg tgaaacttca ccagcagctc
    97201 ttgctgatct aggtgttcat tatgtgatca tcggacattc agaacgtcgt gaatatttcc
    97261 atgaaacaga cgaagacatc aataaaaaag cgaaagctat ctttgcaaat ggtatgacac
    97321 caatcctatg ctgtggcgaa tctttagaaa cctatgaagc aggcaaaact gctgaatgga
    97381 tcgaaggaca aattacagct ggtttagctg atctttcagc tgaacaggtg tcaaacatgg
    97441 ttattgctta tgaaccaatc tgggcaatcg gtactggtaa atcagctgac gcacaaatcg
    97501 ctgacgaaat ttgtggagtt gtacgtcaaa cagttgaaaa actttatgga aaagaagtat
    97561 cagaagcagt tcgtatccaa tacggcggtt ctgtaaaacc agaaaatatt gctgaataca
    97621 tggcaaaaga aaatgttgac ggtgctttag tcggtggcgc aagcttgcaa gcagattcat
    97681 tcttgtcatt gttagacgct gtaaaataat tgttgaaaat ttgcgaaaaa tgcaagaaat
    97741 gtttgcacta atgagcaaaa ttaactaaaa tcgtatatga gggaaatccc ttaggaaaaa
    97801 acaaactcaa aggagagaca aaacatgtca attattactg atgtttacgc tcgcgagatc
    97861 ttagactcac gtggtaaccc aacaatcgaa gtagaagtat acacagaaag cggagctttt
    97921 ggccgcggaa tggttccttc aggtgcttca actggtgaat acgaagcagt tgaattacgt
    97981 gacggagaca aagctcgtta tggcggtaaa ggtgtaacta aagctgtcga caacgtaaac
    98041 aacatcattg ctgaagcaat cattggctac gatgttcgtg accaaatggc tatcgacaaa
    98101 gctatgatcg ctttagacgg aactcctaac aaaggtaaat taggtgcgaa cgctatttta
    98161 ggtgtttcta tcgctgttgc acgtgcagct gctgactacc tagaagtacc tttataccac
    98221 tacctaggcg gattcaacac aaaagtattg ccaacaccaa tgatgaacat catcaacggc
    98281 ggatcacatg ctgataactc aatcgacttc caagaattca tgatcatgcc tgttggcgct
    98341 ccaacattca aagaagctct acgtatgggt gctgaagtat tccacgcatt agcttcaatc
    98401 ttgaaagctc gcggattagc aacttctgtt ggtgacgaag gtggatttgc tccaaacctt
    98461 ggttcaaacg aagaaggttt tgaagtaatc atcgaagcaa tcgaaaaagc tggctatgta
    98521 cctggtaaag acgttgttct tgctatggat gctgcttctt cagaattcta cgacaaagaa
    98581 aaaggtgttt acgtactagc tgattcaggc gaaggcgaaa aaacaactga cgaaatgatc
    98641 aaattctacg aagaattagt ttctaaatat ccaatcatct caatcgaaga cggattagac
    98701 gaaaacgact gggacggatt caagaaatta actgacgtat taggcgacaa agttcaatta
    98761 gttggtgacg acttgttcgt aacaaacact caaaaattat ctgaaggtat cgaaaaagga
    98821 attgctaact caatccttat caaagtgaac caaatcggta cattaacaga aactttcgaa
    98881 gctatcgaaa tggctaaaga agctggctac acagcagttg tatctcaccg ttctggtgaa
    98941 acagaagatt caacaatctc tgatatcgct gttgcaacaa acgctggcca aatcaaaact
    99001 ggttctctat cacgtactga ccgtattgct aaatacaacc aattactacg tatcgaagac
    99061 caacttggtg aagttgcaga atacaaaggt ttgaaatctt tctacaactt aaaaaacaaa
    99121 taattctttt ctaatgaaga tacatgatcg actattgaaa gatagtcgag tatgatcact
    99181 tagttaagta atcgaactct ctacttgctg aaatatcagc aggtagagag tttttttgca
    99241 actagcttct gtatatgatg tttacaaaga atggtacaag aaaa
//
LOCUS       DADVXU010000002        76137 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.002, whole genome shotgun sequence.
ACCESSION   DADVXU010000002 DADVXU010000000
VERSION     DADVXU010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 76137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.002"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(1..254)
                     /locus_tag="KB953_000102"
                     /pseudo
     CDS             complement(1..254)
                     /locus_tag="KB953_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336126.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
     gene            complement(457..936)
                     /gene="rlmH"
                     /locus_tag="KB953_000103"
     CDS             complement(457..936)
                     /gene="rlmH"
                     /locus_tag="KB953_000103"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBB6865109.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            1364..2653
                     /locus_tag="KB953_000104"
     CDS             1364..2653
                     /locus_tag="KB953_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBB6865110.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(2692..3297)
                     /locus_tag="KB953_000105"
     CDS             complement(2692..3297)
                     /locus_tag="KB953_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HBB6865111.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(3502..3966)
                     /locus_tag="KB953_000106"
     CDS             complement(3502..3966)
                     /locus_tag="KB953_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBB6865112.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(3988..4308)
                     /locus_tag="KB953_000107"
     CDS             complement(3988..4308)
                     /locus_tag="KB953_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HBB6865113.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(4452..5531)
                     /gene="pepA"
                     /locus_tag="KB953_000108"
     CDS             complement(4452..5531)
                     /gene="pepA"
                     /locus_tag="KB953_000108"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HBB6865114.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            5731..6081
                     /locus_tag="KB953_000109"
     CDS             5731..6081
                     /locus_tag="KB953_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBB6865115.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            6304..7251
                     /locus_tag="KB953_000110"
     CDS             6304..7251
                     /locus_tag="KB953_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBB6865116.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(7308..8726)
                     /gene="pepV"
                     /locus_tag="KB953_000111"
     CDS             complement(7308..8726)
                     /gene="pepV"
                     /locus_tag="KB953_000111"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HBB6865117.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            8875..9720
                     /locus_tag="KB953_000112"
     CDS             8875..9720
                     /locus_tag="KB953_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HBB6865118.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            9724..10164
                     /locus_tag="KB953_000113"
     CDS             9724..10164
                     /locus_tag="KB953_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HBB6865119.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            10214..10408
                     /locus_tag="KB953_000114"
     CDS             10214..10408
                     /locus_tag="KB953_000114"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865120.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            10544..11221
                     /locus_tag="KB953_000115"
     CDS             10544..11221
                     /locus_tag="KB953_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBB6865121.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTRFRWLR
                     LTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDYFED
                     IQDRPIK"
     gene            complement(11629..12318)
                     /locus_tag="KB953_000116"
     CDS             complement(11629..12318)
                     /locus_tag="KB953_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865122.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(12293..14422)
                     /locus_tag="KB953_000117"
     CDS             complement(12293..14422)
                     /locus_tag="KB953_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HBB6865123.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(14470..15807)
                     /locus_tag="KB953_000118"
     CDS             complement(14470..15807)
                     /locus_tag="KB953_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HBB6865124.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(16059..16418)
                     /gene="rplT"
                     /locus_tag="KB953_000119"
     CDS             complement(16059..16418)
                     /gene="rplT"
                     /locus_tag="KB953_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBB6865125.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(16471..16671)
                     /gene="rpmI"
                     /locus_tag="KB953_000120"
     CDS             complement(16471..16671)
                     /gene="rpmI"
                     /locus_tag="KB953_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBB6865126.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(16743..17267)
                     /gene="infC"
                     /locus_tag="KB953_000121"
     CDS             complement(16743..17267)
                     /gene="infC"
                     /locus_tag="KB953_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBB6865127.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(17311..17436)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            17587..18219
                     /locus_tag="KB953_000122"
     CDS             17587..18219
                     /locus_tag="KB953_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBB6865128.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(18221..19126)
                     /locus_tag="KB953_000123"
     CDS             complement(18221..19126)
                     /locus_tag="KB953_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBB6865129.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(19211..19870)
                     /locus_tag="KB953_000124"
     CDS             complement(19211..19870)
                     /locus_tag="KB953_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBB6865130.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            20186..21130
                     /gene="mvk"
                     /locus_tag="KB953_000125"
     CDS             20186..21130
                     /gene="mvk"
                     /locus_tag="KB953_000125"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HBB6865131.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            21123..22100
                     /gene="mvaD"
                     /locus_tag="KB953_000126"
     CDS             21123..22100
                     /gene="mvaD"
                     /locus_tag="KB953_000126"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HBB6865132.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            22113..23198
                     /locus_tag="KB953_000127"
     CDS             22113..23198
                     /locus_tag="KB953_000127"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HBB6865133.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            23195..24259
                     /locus_tag="KB953_000128"
     CDS             23195..24259
                     /locus_tag="KB953_000128"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HBB6865134.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(24508..25311)
                     /locus_tag="KB953_000129"
     CDS             complement(24508..25311)
                     /locus_tag="KB953_000129"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HBB6865135.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(25331..26173)
                     /gene="kduI"
                     /locus_tag="KB953_000130"
     CDS             complement(25331..26173)
                     /gene="kduI"
                     /locus_tag="KB953_000130"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HBB6865136.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(26189..27196)
                     /locus_tag="KB953_000131"
     CDS             complement(26189..27196)
                     /locus_tag="KB953_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBB6865137.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(27203..27847)
                     /locus_tag="KB953_000132"
     CDS             complement(27203..27847)
                     /locus_tag="KB953_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HBB6865138.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(27947..28705)
                     /locus_tag="KB953_000133"
     CDS             complement(27947..28705)
                     /locus_tag="KB953_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBB6865139.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(28832..29983)
                     /gene="fucO"
                     /locus_tag="KB953_000134"
     CDS             complement(28832..29983)
                     /gene="fucO"
                     /locus_tag="KB953_000134"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HBB6865140.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(30065..30376)
                     /gene="rhaM"
                     /locus_tag="KB953_000135"
     CDS             complement(30065..30376)
                     /gene="rhaM"
                     /locus_tag="KB953_000135"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HBB6865141.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(30378..31223)
                     /gene="rhaD"
                     /locus_tag="KB953_000136"
     CDS             complement(30378..31223)
                     /gene="rhaD"
                     /locus_tag="KB953_000136"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HBB6865142.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(31237..32493)
                     /gene="rhaA"
                     /locus_tag="KB953_000137"
     CDS             complement(31237..32493)
                     /gene="rhaA"
                     /locus_tag="KB953_000137"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HBB6865143.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(32490..33950)
                     /gene="rhaB"
                     /locus_tag="KB953_000138"
     CDS             complement(32490..33950)
                     /gene="rhaB"
                     /locus_tag="KB953_000138"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HBB6865144.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            34103..34972
                     /locus_tag="KB953_000139"
     CDS             34103..34972
                     /locus_tag="KB953_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HBB6865145.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(35098..35997)
                     /locus_tag="KB953_000140"
     CDS             complement(35098..35997)
                     /locus_tag="KB953_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6865146.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(36162..36986)
                     /locus_tag="KB953_000141"
     CDS             complement(36162..36986)
                     /locus_tag="KB953_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBB6865147.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(36997..38292)
                     /locus_tag="KB953_000142"
     CDS             complement(36997..38292)
                     /locus_tag="KB953_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HBB6865148.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(38308..38787)
                     /locus_tag="KB953_000143"
     CDS             complement(38308..38787)
                     /locus_tag="KB953_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HBB6865149.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(38793..39821)
                     /locus_tag="KB953_000144"
     CDS             complement(38793..39821)
                     /locus_tag="KB953_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HBB6865150.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(39838..40944)
                     /locus_tag="KB953_000145"
     CDS             complement(39838..40944)
                     /locus_tag="KB953_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBB6865151.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(41173..43770)
                     /gene="adhE"
                     /locus_tag="KB953_000146"
                     /gene_synonym="adhC"
     CDS             complement(41173..43770)
                     /gene="adhE"
                     /locus_tag="KB953_000146"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBB6865152.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(44075..44455)
                     /gene="yajC"
                     /locus_tag="KB953_000147"
     CDS             complement(44075..44455)
                     /gene="yajC"
                     /locus_tag="KB953_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBB6865153.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(44552..45697)
                     /gene="tgt"
                     /locus_tag="KB953_000148"
     CDS             complement(44552..45697)
                     /gene="tgt"
                     /locus_tag="KB953_000148"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBB6865154.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            45915..46613
                     /locus_tag="KB953_000149"
     CDS             45915..46613
                     /locus_tag="KB953_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBB6865155.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(46781..49420)
                     /locus_tag="KB953_000150"
     CDS             complement(46781..49420)
                     /locus_tag="KB953_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBB6865156.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(50039..51070)
                     /gene="queA"
                     /locus_tag="KB953_000151"
     CDS             complement(50039..51070)
                     /gene="queA"
                     /locus_tag="KB953_000151"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBB6865157.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            51263..52438
                     /locus_tag="KB953_000152"
     CDS             51263..52438
                     /locus_tag="KB953_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HBB6865158.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            52442..53083
                     /locus_tag="KB953_000153"
     CDS             52442..53083
                     /locus_tag="KB953_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6865159.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            53080..53997
                     /locus_tag="KB953_000154"
     CDS             53080..53997
                     /locus_tag="KB953_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HBB6865160.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            53994..54665
                     /locus_tag="KB953_000155"
     CDS             53994..54665
                     /locus_tag="KB953_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6865161.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(55206..55694)
                     /locus_tag="KB953_000156"
     CDS             complement(55206..55694)
                     /locus_tag="KB953_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HBB6865162.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(55895..56161)
                     /locus_tag="KB953_000157"
     CDS             complement(55895..56161)
                     /locus_tag="KB953_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HBB6865163.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(56161..56424)
                     /locus_tag="KB953_000158"
     CDS             complement(56161..56424)
                     /locus_tag="KB953_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBB6865164.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            56900..57085
                     /locus_tag="KB953_000159"
     CDS             56900..57085
                     /locus_tag="KB953_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBB6865165.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(57227..59050)
                     /locus_tag="KB953_000160"
     CDS             complement(57227..59050)
                     /locus_tag="KB953_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HBB6865166.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(59648..62353)
                     /locus_tag="KB953_000161"
     CDS             complement(59648..62353)
                     /locus_tag="KB953_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HBB6865167.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(62659..63606)
                     /locus_tag="KB953_000162"
     CDS             complement(62659..63606)
                     /locus_tag="KB953_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HBB6865168.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            63796..64065
                     /gene="rpsN"
                     /locus_tag="KB953_000163"
     CDS             63796..64065
                     /gene="rpsN"
                     /locus_tag="KB953_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBB6865169.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(64241..64603)
                     /locus_tag="KB953_000164"
     CDS             complement(64241..64603)
                     /locus_tag="KB953_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBB6865170.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(64614..65735)
                     /locus_tag="KB953_000165"
     CDS             complement(64614..65735)
                     /locus_tag="KB953_000165"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBB6865171.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(65751..66101)
                     /locus_tag="KB953_000166"
     CDS             complement(65751..66101)
                     /locus_tag="KB953_000166"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBB6865172.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(66231..67742)
                     /locus_tag="KB953_000167"
     CDS             complement(66231..67742)
                     /locus_tag="KB953_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBB6865173.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(68064..69416)
                     /locus_tag="KB953_000168"
     CDS             complement(68064..69416)
                     /locus_tag="KB953_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBB6865174.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(69569..70645)
                     /locus_tag="KB953_000169"
     CDS             complement(69569..70645)
                     /locus_tag="KB953_000169"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBB6865175.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            70841..72586
                     /locus_tag="KB953_000170"
     CDS             70841..72586
                     /locus_tag="KB953_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HBB6865176.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(72866..73303)
                     /locus_tag="KB953_000171"
     CDS             complement(72866..73303)
                     /locus_tag="KB953_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HBB6865177.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(73287..74660)
                     /locus_tag="KB953_000172"
     CDS             complement(73287..74660)
                     /locus_tag="KB953_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865178.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(74982..75470)
                     /locus_tag="KB953_000173"
     CDS             complement(74982..75470)
                     /locus_tag="KB953_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB6865179.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(75492..76094)
                     /locus_tag="KB953_000174"
     CDS             complement(75492..76094)
                     /locus_tag="KB953_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBB6865180.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        21833 a        16267 c        13927 g        24110 t
ORIGIN      
        1 ataattgata gcagctttgg tatcatcatt agaatctata taccttttca aaaacatttt
       61 aaaaaactca tcagataaat tgtgggtttc atttggagca aacaaaaaag ctaaaaaatt
      121 actatgtctt atttcatttt tagatagctt aagtatctga aatatatttg ttttcccagt
      181 ccatttagat agttttcttc aatatcatcc atatcctcta atagattttt taaacacttt
      241 ttttcatcta tcatgttttt tctccttgcc attattctaa ctaaaatttt acgtatttgt
      301 ttctatgtct acattatctt tttaacagta tctatttttc ttttgcaact tacttggcta
      361 ccaaagcaaa taaaaaagtt atttataaat ttctccctac cactacctat tgctattatt
      421 taaaataggg ataatttttt ctcttaaaaa atatcattat ttgtggtatg gagcccccgc
      481 attgatccgg aaggcacggt agatttgctc gacaaggatc agacgcatca attgatgtgg
      541 atacgtcatt tttccaaaag agatctgagc attgcttctt ttcatgacgg cttcgctgag
      601 tcctaaagag ccgccgataa caaaaacaag gtggcttttt ccttggatac ctaattggtc
      661 gatttgttta gcaaaagatt cacttgtagg atttttccct tcaattgcta aggcatagac
      721 gtattcttgt tctttgattt tcgctaagat ccgttcccct tctttttctt ttacttgtct
      781 catttgcgct tcgcttaaat tctccggggc tttttcgtca ggaacttcga tcaattcgat
      841 ttttgcatag gcattcagcc gtttgacata ctcattgata ccttggatca ggtatttttc
      901 ttttaatttt cctactgaga taattttgat attcatgttt tctctccttt ggtcttcatg
      961 ctcactttgt ttattataat caattttttc gtctatatgt ttattttggc tatttttgtt
     1021 aaaaagattt ccccaacagt tatgcacaac atccacaggt ttatccacag aaaacagtgt
     1081 tattttacct tatttcatca cattcattag gcaaacatac gttttataag aatttatcca
     1141 ctttatacac aggctgtgga tatgttttat cccttatttt tccaatccta tccgcgtagt
     1201 tttacacagt atctgtggaa aaagaaaatc aaatattttc atctgaaaac aatgattttt
     1261 cctaaagttt ttcataaaaa gttcacagtc tcaaagtaga ctaaagtcaa caaataagac
     1321 tatactaaca gtagataaat gaatgtaatt ggaggaggaa cacatggata gaaaaaatgt
     1381 gacaccaaag atgaaaaaaa gtaaaaataa tagcctctgg cgtaagttag gtctggggct
     1441 tgttggaggg atcgttggag gactactgac agctggcatt ttttatgcag cgatgggcac
     1501 tggcaacaat agttctactg ctacaagcgg caatcagaat tctgccggtg aaacagttgt
     1561 ggaaaatgtc aaagtcaatg tagattctga tatcaccaaa gcggtagata aagttcaaga
     1621 cgctgtcgtt tctgttatca acttacaaag tcaaaaccaa tcaagcggtt ttggcggact
     1681 attcgggcag caagaagaaa gtagcagctc agatgatagt aatttggaag cttatagtga
     1741 aggaagcggc gtgatctaca aaaaagacgg gaatactgct tatgtagtca cgaataatca
     1801 cgtagtagat ggccagcaag gtcttgaagt cttgatgaaa gacggtacga aggtaaaagc
     1861 tgaattagtt ggaacagatg cttattcaga tttagcggtg ttgaagatca attcagacaa
     1921 agtcgaaaca gttgcctctt ttggtgactc aagtgctctg aaggttggtg agcctgcgat
     1981 tgcgattggt tccccactag gatctgaata tgcaaattct gtcacttcag gaatcatttc
     2041 ttccttgaac cgacaagtga caagtacgaa tgaatcgaac gaaacagtca atatcaatgc
     2101 gatccaaaca gatgcagcaa tcaaccctgg aaattctggt ggtcctttag ttaatatcga
     2161 aggacaagtt atcggtatca actcaagtaa gatcgcaagt acttctgaat ctacttctaa
     2221 tgtaagcgta gaaggaatgg gatttgctat tccaagtaac gatgtagtga atatcatcaa
     2281 tcaactggaa aaagacggaa aagtgacacg tccagctcta ggtatcacaa tggtcgactt
     2341 atctgccgtt tctactcagc aacaagaaca aatcttgaag attccttcat ctgtaactaa
     2401 cggtgtcatc gtgacaaatg tccaaacagc tacaccggct gaaaaagctg gattgaaaca
     2461 atacgacgtc attactaaga tcgatgacaa agaagtttct tctggtgtag aacttcaatc
     2521 tgctttgtat aagaaaaaag tgggagattc tgtcaaaatc acttactatg aaggctcgaa
     2581 agaaaaaaca actactgttg aattgacgat cgatcaatcg gcattaaaac aaaatcaaaa
     2641 tagtgaaaat taataaaaaa gaggttgtga caaagacttg tcacaacctc tttattttgg
     2701 aaatgcttct ccgactacgg catcctcagg caatactaag attccttttt ttgctggagc
     2761 atttggcaaa tgcaattcac gagcggaaca gatcattcca tagctatcta cccctcgcaa
     2821 agttcctggc cagatcatca ttccatctgg catcatagct cctggttttg caacgacaac
     2881 tttttgtcca gcttcgatat tgggtgcacc gcaaacgatt tgcaatactg taccattgtc
     2941 tacttctgtc tcagtaatcg ataaatggtc agaatcagga tgtgggacgc atgtttttac
     3001 gtacccgaca acgatcttcg gttcatgatc agcttcaagc tgttcttcga atcctgcttc
     3061 tttgatcaat cgattcaatt tcgctaattc ctcatctgtt aagcttactt ggccgttccc
     3121 ttcgattgtt agatgatcgg agatatggaa aaagttccaa gcaacaactt gaccttgttc
     3181 atctttgatc cgagcaacat tccatttgcg ttcaacagtg ttttctgttc ctttatcgtc
     3241 ggctataata accatcaagg tgtctccgac gtgtgctttg ttataagcaa aaatcatgtt
     3301 ctttcctcca ttatcctttg ctgagattta tcttaacaaa aaattgcatt cactgcatga
     3361 aatttttttc atgctttatt tccgaagaag cctcttctgg aacaccattt attcgtaaca
     3421 agggactgtg acaagacttt tgtcacagtc cctttactgt tttagttaat tgattaaaaa
     3481 ggtcgtacaa gttccacttg cttaattact tggctgttct tcttcgtcag aaacgatcaa
     3541 gacatcacaa ggtgaatgac gtatcacgta gctggctaca cttcctgtca taaagcgttc
     3601 gactgcattt agacctgact gcccaaccat gattagatcg atctcatatt tttttggcaa
     3661 tttgactgcc atggattcac gaacagaacc atatgcaata attccttcta tatttgtgta
     3721 atcaacagat ttcccatatt ctttacattc ttcgatcatt tccttggctg tttcagtttg
     3781 ctgatcgact agattttgat caataggtgc atagcccata aagttataaa attgctgatc
     3841 gattacgttt gctacatata ctttcccatt attacggcga gccacttcta ccgctttttt
     3901 gaaagcagca agtgcttgtt cactaccgtc gatacctact aaaatattct tatattcttg
     3961 attcatcttg acccctccta acagatgtca gccttttgtt acactttcta ggaacgattc
     4021 gatttcctgt tttgttttac gatctttatt cactagacga cctaattctt ttccgtcttg
     4081 gatcacgaca aaacttggga taccaaaaat gttccattca gcggctacat ctatgtactt
     4141 gtcacggtct acttcaataa attgccaatc agaaaagtca ttttcaattt caggcatttg
     4201 cggtttgata aaacggcagt ctccgcacca gccggcagta aagaaaaata cactctttcc
     4261 agtttctacg tagcctgcta attcttctaa actatttggg ataatcattc tttgtcctct
     4321 actttctgtt tatgtatttt tcaattaaat tatacacctt tctttcgaac taacaaacat
     4381 tgatcctctt tttataaaaa aaatctggca aaaagcaatc ttttgccaga ttttatagtt
     4441 tcactatcgc attattttcc gtatacgatc gtatttactg ttgatttatc caaacctttc
     4501 agtgtttgaa tcaacatttc tcgtgcagct tcaaaatcgc gaatactgaa catcgtttga
     4561 tgcgtatgga tataacgtcc cactacgcca atcactgtac ttgggattcc ttcgttttgt
     4621 gtatgagcag cacctgcatc tgtaccgcct tttgaaacaa agtattgata agggatattg
     4681 tttgtttcag cgatatccag caagtactcg cgcaaacgag gaagcatgat caaacctgga
     4741 tcttggatac gcatcaatgt accttctcct aaatgtccga atgtgccatt tttcgtcact
     4801 gtatcatctg ctgcagagca gtctactgca aagaacagat ctggcttgaa tttattgaca
     4861 gaagctttag aaccacgcaa gcctacttct tcctggacat tcgcgcctgc aatcaatgta
     4921 tgacctaatg tttcattttg caacgcttct aaagcttcta ataccattgt acagccatag
     4981 cggttgtccc atgatttact gataatattc tttccgttgg ctgttttgat cgtttcagcg
     5041 taaggaacga ttgtgtctcc tggaaggaca ccgtaactca ttgcctcttc tcttgattca
     5101 aacccagcat cgaacaaaat atcggttact tctactgatt tttgtccgct tgttcctcgc
     5161 aataaatgtg ggggaacgga agaagaaata catggataat tcttaccagt agaggtttta
     5221 agtgtaaaac gctgagcaga aacaacgtaa ggattccagc cgcctagagg tacgacttta
     5281 aaaagcccgt tttcttggat ttgtgtcagc atgaatccga cttcatccat atgtgctgcg
     5341 accattacac ggggtgcatc cgcttcttca tgatggcgca gaccaaaaat gccgcccaaa
     5401 ccatctaact gtaactcatc taccagcggt tccatatgac tttccatata agcgcgaaca
     5461 tcttgttcaa aaccgcttgt tccttgtaat tctgttaact ctttgattcg ttgaaatgtt
     5521 ttttcttcca tatgctattc gtctaaattt cagactaaac tcctctcaga cttatattga
     5581 tctgcggata aaatatgatt ttttctgcag accgatcgta tttattatag cttatttttt
     5641 ctggtagcgg ttatttattt caaagaaaat atgagagtgt ggtaaaataa gcacaaggag
     5701 taacaagtga gaagaaggag gtatgtccac atgtatgaag aaagcgaact aagctatttt
     5761 aaaggcgggt tagcagttgg aatcggtcta ggattattga gcggggtagc ttcaacgatt
     5821 tggtatcatc gcaaaaaagt attgtcagca gaccaagtac ttgaaaatat caagtcatct
     5881 tttctaaaag aaggtgcaat cgaaggttct tggatctctt ttgaaaagga acctattagg
     5941 aaattcgccg ttcggtcgag tgcttatcgg ggaggtcttt ctcgaataga agatggcgaa
     6001 actgtttatt atgaattttt agcagatgct catacaggaa ccgtattaga aatttcaaga
     6061 aaaaaaggtg caacagatta atacgtcaaa aaagtgagag aacgaatacg cacccactat
     6121 tcaaaagaga ggtatcatgt ccttcttttg tagtgggtgg ttttttagat agagctttcc
     6181 aaacatatat tccagtattt tctttttaaa taaatgatat ttagcgggaa aaactagcat
     6241 ttcctttgat tatgaggtat ttttaattta gcttttgttt tctatagtta aggagagtga
     6301 aaaatggaac aacaagaaaa aattgctgtt ctgattcctt gttacaatga ggaagcaaca
     6361 atttcaacag tcattgcaga ctttaaacga gaattaccag aagcagatat ttatgtctat
     6421 gataataatt cgaccgatcg aacatacgaa ttggccgttc aaggtggagc aatcgtaaaa
     6481 aaagaacctc gtcaaggaaa aggaaatgtg atccgccaaa tgttctttga tatcgatgcc
     6541 gactattatt taatggtaga tggcgatgat acttatcctg cagaagctgt acatggtttg
     6601 ctcgagaaat tacgttctgg tgaagcagat atggtcattg gtgatcgcct atcaaatgga
     6661 acctactttg atgagaataa gagacctttc cacgattttg gaaataacct tgttagaaat
     6721 acaattacta gaatgtataa aacaaaaatt cttgatgtca tgaccggcta tcgaggattc
     6781 aatcgtatct ttgtcaaaag ttttcctatt atgagttctg gttttcaaat cgaaacggaa
     6841 ttgacgatcc atgctttaga taaaaaattc aagttcgttg aaatcccaat cgattatcgt
     6901 gatcgtcctg aaggcagcga atcaaaattg aatacattct ctgacggatt caaagtgatc
     6961 atgatgatta ttaaaatgtt taaagattat aagcctttgc ttttctttgg gatttggacc
     7021 atcttcttct ttttatttgg attggtcacg gggattcctg tgattcgcga atatatgctg
     7081 acaagcttta ttacacgcat cccttctgcg attttaagta ctggattgat gactttagca
     7141 cttctttcat tagtaacagg gttgattttg gatacagtcg tcacgaacgc taagaaagaa
     7201 tatgaattga atctatatca tgtttatcat gaataccaca gtagaaagta aaaaaaggat
     7261 caagactgca aaaataattt gcaattcttg atcctctttt taattaatta tttcgccaaa
     7321 cgatagatgg catctgcata gattgctgtt gctctgaaca agtcatcaag actcataaat
     7381 tcgtttgctt gatgcattgt atcagtatat cctgggaaca tcgctccata agcaacgcca
     7441 cgtttcaaca aacgtccgta agttccgcca ccgatgattt gttcgtagcc tttttctcca
     7501 gtgtggtctt catagacttg caataatgta gccaccaatg gatcatccat tggtacgtaa
     7561 tgtggttcca ttacgcgagc acctcgagta actgttgcgc cttcttgccc cactgtttgt
     7621 tcgatgtcac tttgcaatcc gtctactgtt acaccttttg gaaaacggaa attcatattg
     7681 atgaagttcg cttcctctgt accattttct tcaaaagcaa atagtccagc attcattgtc
     7741 aatttgccca ttttttcatc ttcataagcg actcctagtt tttctccata aaaatcttca
     7801 tggacataag cagctgctac atggataaat tgtttagcag atcctaggaa ttcatagtta
     7861 tctaagaaca atgccaagaa agaacctgca ttgataccag attgtgggct tgcaccatgt
     7921 gcgcctttac caactaattc gatttttaca tacgtattgt ccgcttcaat cgttccgctt
     7981 actggatttg cttcgatgaa ttggtaaaat gcttcttcca tcgcgatagc tgcatctgca
     8041 cttggtactt gaagtgctgc agttgctgtt cctggcacca tattttcgcg aagtccggaa
     8101 acaaaagatt tcaagacata atctcctgca ttgtcgcctt tgaagcgtaa agctaaagaa
     8161 acatttcctt tttcaccatt gatgattggg aattcagcat ctggtgagaa accaaaatct
     8221 ggttcttctt catgttcgaa ataataagcc atatcgcccc agccgctttc ttcatcgctg
     8281 ccgactacga agcgaacttt ttttgatagt ttcaagtcaa gttctttaat gatcttcatc
     8341 gcatagtaag ctgccatact tggacctttg tcatcgcttg atccgcgtgc aaagatcttt
     8401 ccatccttga ttactggttc ataaggatct gtgtcccagc catctcctgc aggaacgaca
     8461 tccatgtgtc caaatatccc tagcgtttcg tctccttcgc caagatcaat atgccctgca
     8521 tagttatcga cattttttac tttaaatcca tcacgttcgc cataagctaa catatgtttc
     8581 aatgcgtcgc gaggtccagg tccaaatggt gcatctggtg ttacttttga gtcatcgcgc
     8641 tcgctgttga cacgtaaaag atttttcaag tcttcaagca agtcttcttt acgtgcttct
     8701 acttcttttt gccaatcaat tgtcataaag ttaatcctcc acttatttaa gtcttcgtgt
     8761 tcatgatacc acaaattaca gaaaaaaata gttcaaaaaa tctgacaatt ccttttctta
     8821 tctgtttttc tctaaaaaaa atatacgcta taatgagtaa aaggaggttt ttagatgctg
     8881 ataaacgaac aactagtgaa aagagtcatc acacctcgtc caaaaatttc acataaagga
     8941 acatttggac gtatcgtttt gattggcggc aatgaacaat atggcggagc aatcattatg
     9001 agtgctttag ctgccgtaca cagcggagcg ggtctcacga ctgtagtaac tgatgaaaaa
     9061 aatcggagtg ctcttcacag tcatctgcca gaagcaatga ttgtagtttg gacggatact
     9121 gaccagtttc tttccgtaat aaagcaagca gatgtactct tgattggtcc cggattgggt
     9181 acttcttcta agagtttgga acgtttcaaa caagtactag aaaaccaatc aaaagaacaa
     9241 tggctggtga ttgatggctc tgctttaagt ttacttgctg ctaatcatct tttgctaccc
     9301 tatcccgaac aaactgtact tacacctcat caaatggagt ggcaacggtt gagttctcta
     9361 ccgatacaag aacaacggga taaagaaaac aaaaagaagc aacaggaaat cggtgctgtg
     9421 ctcgttttga aaagtcatga gacaaaaatt tatggtcacc agcacttcta caccaatcct
     9481 ttaggttctg ctgctatggc aacgggcggc atgggtgaca ctttagctgg gatgatcaca
     9541 ggatttttag cccagtttcc tcaaaatgac caaacgattg cagcagctat ttatcttcat
     9601 agtttcattg gagaccagct ggcaaaagaa caatatattg ttttgccgac acaaatcagc
     9661 gaagcacttc cgaaatggat gaattatttt tgtaaaaaat caatggaaaa cgaggcatga
     9721 gtcatggaga aatttacagg ttatgtgcga gaaccttttt attatgaaac agatcaaatg
     9781 gggattattc atcacagcaa ttatatccgc tggtttgaag aggctcgggt agccttattg
     9841 gtacatctag ggtttcccta taaaaaaatc gaagaatctg ggatcatcat ccccgttctg
     9901 gaagtaaatt gcagctataa agaaatgatc cattacggag aaaaagtgag catccatcca
     9961 actatccaaa agtatacggg gacacggttg gattttagct acgaaattgt gggaactgaa
    10021 gacaaaaaat taaaaactac tggcagtagt aaacactgtt ttttactttc tgattcgcaa
    10081 cgattagtga agctgagcaa agtcaatccg gagttagatc agttattcca agcgtacgct
    10141 tctttggatg atgtaaattc ttaataaaga ccgtctaagc atttttcttg tattgtgtcg
    10201 agtagttcgt ataatgaaaa gaaaaaagca ggtgatcccc atgtatgtcg ttaaagtaat
    10261 gcacggttac atcgataaaa ctggctgtcg cactcgggaa aaaaacttag acaatctgct
    10321 cattttcaaa gacaaaaaag aatccgaagc ttttgctaaa agaatcggcg gtcgtgtcaa
    10381 accgatccaa gaggtacgac cagattgatt caatattttt cttttcgagg ctctaatagg
    10441 catgcctatc agggcttttt ttgcagactg tgtttttatg cccacacgat ttgtaagcga
    10501 tttatgttat aatagattat atctgcatag aaaggaaggt taaatggtta tcaataattt
    10561 gcaacagcaa gcttatgaat ctatacgtaa acaaatcatt tattccgagt tagcacctgg
    10621 aagtaaaatc tccgataaag tgttagaaga acaactagct atcgggcgta caccgattcg
    10681 agaagcactg atccaactgc gcaatcaaga gcttgtaaaa accattccac agtccggcac
    10741 ttatgtttca aaaatcgata tccgatctgc ttcattagcc cgttatacgc gggaaaagtt
    10801 ggaaaaaccg atcctgcaag aatgcagtgc gaaaatgacc aagcaagatc aagctaaact
    10861 agaaaaaata ctagaacaaa ccgatcaggc agtaatcgct caaaataaaa aagctttttt
    10921 tgatttggat actgattttc accgaacttg ttatgaaatt gctggaaaac gagaaatttg
    10981 ggattggcta gaaagttata gtacgcgttt cagatggtta cgtttgacga tttcagaatt
    11041 agactggggt cgagtattag atgaacatca gacaatgctt caatcaatga ttgatcataa
    11101 ctttgatgaa gtcggatttc tttgcacgat gcatttacat atgattatcg aggagcaaga
    11161 atacgtgatt cataattacc ccgattattt tgaagatatt caggacagac ccatcaaata
    11221 agaggttttg gaatcagcgt tttgacctga gtattagaga aagaaacggc aaaatagttc
    11281 ctcatatttt gccgtttttt ttgagctaat accgagggtc agctgattgc acaccgttta
    11341 ttgagaggtt gtgaaaaagc tgccctgcat caagtaataa gaacagccaa gtaaaaatag
    11401 cttcttatgt ttttaacttg gctggactta ttaccgcaga tgcaagcttt tgaacaccgt
    11461 tttccaagag gtcgtgagcc tgccgttcat ctccgagtca caaggaacaa tttcaaaaaa
    11521 cagcttctca tgtttgttga atattttgac ttgtgaccga ggagttgaca ggagaacatc
    11581 gtttattcgg aaaaaaagga ctgtgacacg acttttgtca cagtctcttt atttgtaatc
    11641 taaatcatac tccgtacatc ttttttctat tttttttatt tcttctaaga tcccttggct
    11701 acgaatttca ttatatggcc aaagtaagac tgcctctaat acataaggcg aaaaatcgct
    11761 ttgattcatc tcttttgaaa aactcatcgc tgctatctcg ctcaatgcac gaatgtttga
    11821 ttgatgtgta taaaaaggca accgccacag cacaattttt tcgctttgcg tataaattcc
    11881 cttaacagaa gcttcgatgt ggtgaaatag ttcatgtcca ataatcaaag cttgcgctgt
    11941 ttctttagag atcgaagcta gtcctgaaca ttttaatttt tctaacggct ccgtcatcaa
    12001 tcgaatccct tcatcttctg cataagttgc aaaagtgatt cgttcgttta tttgctctga
    12061 ttcacaggaa acgattggaa cagccatttg ttcacaaatt tctcctacat cggcaaaagg
    12121 gtactttttt ttgatgctac atgccgtttc gataccacaa tcgattgatt gttgtataag
    12181 aattttctgc ttggacaaag gaatcttttt ccaaaataaa tctcgccgaa actggtagaa
    12241 tccccaaatt tcttcgggga tctgttgtaa ttcgtttagt aactcttcta acttatgttt
    12301 gtttgatatt tccggcaacg acgatcactt cttcctccgg ttccatagta gatacttcca
    12361 gatccatcaa aagctgtagt tgttccagat ccgtcgctgt aaaatcagag actcttgctc
    12421 ctaagcagag ataaaattct ggtcgggcaa tcaattcagt ttgataaact gccatatctt
    12481 cccaaagttg ttcgacagct gtcatataat tggctgcagt tgttttcata catgaagcat
    12541 gtccacgttg gaccttgata aatccttttt gatcgatgat ccgaagactt ccagcatttt
    12601 ttgaacgatt ctgttcgccg catacataaa agaaaggtgt attttccaat aagcaaactt
    12661 catttttatt taatcgcata tcctctgcag caagctctag tgcttcttct gttgtgatct
    12721 ccttggttag atcggttgct ttcacttccg tagaaccagt agcgatagcc gttacttttg
    12781 aagtctgtgg atcgatttct acgtggactt cgatcgattc tggtgtagcg cctgattcaa
    12841 tcgctttatt catcgcttca tttttcaacg atcgtatatc ttctttactt ggcgaaggga
    12901 tgatacgttc tacaacatct cggaccattg caagtgctac accaatagaa gaaatcactt
    12961 cggcgttttc aggaatcgaa tatttcacac ccattttatt actaaagtaa gtaatcaatg
    13021 aggcagcgcc gccccctaca cctacaagag aaatctgatc tttctccaag tgatacttgt
    13081 ctgccaattc taaaatcacc ggttcaatct tagcatagga tttttccatg atctgttctg
    13141 cgatgtcttc aaccgtcgta tgacaataat cagctaatgc ttggatggct tttcgtgcag
    13201 atgggacatt tccataagaa aaatgttctt cttgcactaa tccaaggaca ttggctgcac
    13261 atgtatttgt aatagtcact tcttctccgt cttccatcac taccttgacg taatcagcag
    13321 ggtctccttc tttaggagag aaaaattcaa ccttaggacc tttgattttt tctgtctccg
    13381 taaatactgc ataatctaga ccggcaatat gtgcagaacg tggaccgaca tcgatgattc
    13441 ctgactgatt agcccgtacc atcgaaccgc ctgccacacc tagaacccga acatctaatg
    13501 aagagatata tgttgggtgg ccacctacaa ttgaataatc gattgctgga cgtccatttt
    13561 taatgacccc gatattggtt gttgttccgc ctacttcaaa ataaacacca ttagacgctc
    13621 tcaagtacat caaagagccc atgactgatg ctgctggacc ggaaagcatg gttaaaacag
    13681 gtcgtttttt catttcattg atttccatta ctccgccgtc tccgcgcata atcatcaaag
    13741 aaacattgac ccctgcttct cgtacggaat cttctgtaga ggttgccgta tctagcattt
    13801 ttggtaaaat agatgcattg attgctgctg tccgagttct tcttgtcaat ccatataatt
    13861 ttgtgatgtc agaagccatc gttgtaggga tcgattttac cgatgctgct tcataaacaa
    13921 ctcgttctgg ttccccatta tctactccaa atgccataga agaaaccaag acctccgctc
    13981 tttctctttc taaagaagaa atcgtttctg aaacacggtc tacatttaag tgtttgacag
    14041 gtaagaatgc attgacaatc tctatttttt tcttattccc taaatcgata tcgttcaatc
    14101 tagtttgtcg ttttgctaaa aaaccttcta atccaccttt tgccatcccg attacgccga
    14161 cttttgctac gtctccttca atcagtgcat tagtcgcttg tgtggtagaa tgtgcaacaa
    14221 acacaacatc ttctggactg atattatttt ccagcaaaca attttgaaaa gactgaacga
    14281 cacctgcggc tactccacgg acatcatcgt gggttgtttt cactgaagat tttccgatga
    14341 tttcgtgcgt cgcattatcg attgcgactg ctttcgtatg tgtaccgcca acatcaattc
    14401 ccattcgcac aaaacgtttc attttctttt cctccattca tctaatcgtt tttatcgacg
    14461 ttttgtttac taggcgttcc cgtacaataa gaacgtgatc aggcataaga tcgctccgat
    14521 gatccaacca gttgggatgg atttcttcat atattctcga gaagttactt ttgtataacc
    14581 taatccccaa gcaacccaag attgtgtaat atctaaatgc tgaggagcaa tcgttgtgat
    14641 tgcaaataac gggaacaaaa aggctggcga catcgttgga ttaacagcta atacgacagc
    14701 taaaatagct gagcctgaac cgactagatt cattggaccg cggaaaaatc ctagtggtgt
    14761 caatatagca aatacgattg ccaagactaa tggtgattgc ggtatccaat ctccaaaaat
    14821 agctgaaaaa taaggagaag catacgcagc tgaattattg aacatcgcta aggtcatcaa
    14881 gaaaccaacc attggcgcaa catctactgc gccatctgta aataatttcg caaactgacg
    14941 acagatttct ttataacctc cgcgcaattt tccagtaaac agcaatgccc agatacctgc
    15001 tagaataaaa cctaagatga ttggaagatc taaaagaacg acccctaaaa ctggtaataa
    15061 tacggctaac catgagatca ttggtgcgtc gccaccttca gctggtacgt ttgctgccat
    15121 tgcatagcgt ttctttttgt tcattgatat attagcgacc gttagtacca caactaagct
    15181 tacaatcatc ccaatcatac caatttggaa atagtcatta taagtatagc tttcagccgc
    15241 tggattgaaa ccagcataaa tcgtttgata ttgtttgaaa ttgacgatat tagcaaagat
    15301 tccagccata atcgagccca taaatgagaa cagtgcaacc ggtactgaaa tacctaatga
    15361 catcaagatt ggcaacacaa taactgcaat agaaatcact ggtccgattc ctgtcatcga
    15421 catgaatata acagcggtta caatacatag taagctcatc gtgattcttg gcttgtcccc
    15481 tccaagttca actacctttc gaatcagggt agctgcgatt ccgctatcta ctagaacacg
    15541 cccgaaaaag gcaccaaaga agacatttac caagatcgat ttggcgtact cagctggccc
    15601 cgtcgtatag atatgcgtca acacatcaat tactccttgc ccttccatcg ccgggtttgg
    15661 tgaaaacgtg tttccgacta gcgctaatcc catccataca gtagccatga tggcaaagcc
    15721 gaccatcaag ttgtagcctt ttacacagta ccagaccatg aaaaagaatg agataactaa
    15781 caaaatacca ataataatat cactcatgtt attcctccgt ccattttgtt tttctgataa
    15841 gcaaactaat atatcagtca aactcattct aacttatgta gttagcgctt tcattaaagg
    15901 gtgaccataa atgttcagtt ttcaattaaa aaaccttaat tatgctattt tagacttata
    15961 cgttattgtc ctgtttcatt tgatggagga ttgtaaataa aaaaagagga aagaacataa
    16021 cttttcgtta tcttctttcc tctttataat tgtttagctt atttagccaa agcttctttt
    16081 gcttggtcag ccaatgcagt aaatgctgct gcatcgttaa cagccaagtc agctaacatt
    16141 ttacggttga tgtcgatttc agcaactttc aaaccgtgca ttaattttga gtagctcaag
    16201 ccattcatac gagctgctgc gttgatacga gcaatccaca atttacggaa gtcgcgtttc
    16261 ttttgacgac gatcgcgata tgcgtagttg tatgaattca ttacttgttc ttttgctgtt
    16321 ttaaataaag tgtgttttga accgtagtaa ccttttgcta attttagcac tcttttacga
    16381 cgtttacgag ttactgttcc acctttaaca cgtgccatgt ataattcctc ctaataaaaa
    16441 taaatcttca gattctgtta tttgtacttc ttatttcatt tttgctagtt gttggcgaat
    16501 acgtttgaag tcgccactag aaaccatgct tgctttacgc aattgacggc gttgtttttt
    16561 tgttttgccg tggaaacggt gacttgtaaa cgcgcggaag cgtttcaatc cgcctttacc
    16621 agtacgtttg aaacgttttg ctgatccgcg gtgtgttttt tgttttggca tgactatatt
    16681 tcctcctcaa aatctttcgt tatccgacta tcggctgtcc aacagccagt tagtcattca
    16741 aattacttac tgtcgttttt cggtgccagc gtcaagaaca tgctgcgtcc gtccattttc
    16801 gctttttgtt ccactgttgc aatatccgca gtttcttcag ctaagcgatt caagactttt
    16861 tgaccaattt ctttatgggt aatggcacgg cctttgaaac ggatagaagc tttcacttta
    16921 tcgccctttt ccaagaactt acgtgcatta cgaagttttg tattgaagtc attcaagtcg
    16981 atcgtaggac ttaagcgaac ttcttttaca ttgatcactt tttgtttttt acgcgcttcg
    17041 cggtctttct tttgttgttc gaaacggtat ttaccgtagt ccatgattcg cgcaactggt
    17101 ggtttcgcac caggggcaac taacacaaga tccaagtttg cttgttctgc aatattcaat
    17161 gcttctgctt ttgtcttaac tcctaattgc tcaccatctg aaccgatcaa tcgtaactct
    17221 cgtgcacgaa tgccgtcgtt aaccatcata tcctttgcta tggtcattca cctccaaata
    17281 ttatgagaga aagaaaagct taccgaataa acggcaataa aaaaacgagt tctcttacag
    17341 aacccgcgcg tacttcacct aatagaacca gtgaaaacta tctagcccag tgacattatc
    17401 aactaaggcg agaagcgggt gcttctgctt ttatttctca accatcatag tatagcaaaa
    17461 actaatcttt ttgtcaatgt ttttcataca gtttgtcttc cttttctgaa aatgattaga
    17521 ttagcagact ttctagcagt gattcccgct tctttgctat actaaagaaa aacgaggtga
    17581 acccacatga aaaaaatcga acctattcca ccttctcttc ctcttcttac aaagggagag
    17641 ttttatttag aagatgagga gatcttctct ggtcttctga tcaacggggg tgaccaaagc
    17701 tatctcgatg ccaaaaatat ggtattaaga gagagtcata tccaaaaact tacgatgcaa
    17761 aaaacagttt tacgtcattt tgaatgcagt aatgtcattt ttgaaaaatg tgatttttcc
    17821 aatctggagt ggattggtgg cagtttccac caagtcgtgt ttcatcaatg taaattaaca
    17881 ggtacgaatt ttgccgaaag ttatcttcga gactgtactt tcactgattg tattgcttcg
    17941 atggcttcct ttagtagtac aaacttgaaa gctgtctcct tcgatcattg tcaattagaa
    18001 gacagcgaat tttatgaggt cacttggaaa aacctttttt tgtctgacaa tcagttgacc
    18061 ggttcgaact ggtttcgaac ttctctgaag ggattagact ttacgactaa tttcttcaca
    18121 gcaatcgctt tatctcaaga ttctttggcg ggactaatcg tcaaccaaga gcaagcactt
    18181 gtgatcgccc aagcccttgg tttgcagatc aaagactaga tcaacgaatc cgccgccaca
    18241 gtgcaataag catccaaata gataagacca aactgatgat caaggcgatt ccccatccga
    18301 aaacggaatg ggtaaagggt aactggagat tcattccgaa gaatcctgtc acaatagttg
    18361 gaacggtcaa gatcagcgac cagaccgtca aaaatttcat cgtgtcattc aagttgttat
    18421 tcagcaaatt attataagtg ccagagagct ggtctaaaat ctgcgaagct aagttcgtca
    18481 tttcaactgc ttgtcgtgct tcgatcagcg catcgtccaa ctcttctttt tccttttctg
    18541 acaacttgcg ataaatagcc aatgccttga tttgttctaa aagtactaca ttttgttttg
    18601 ttcctgtaac taagtaaacc aaaccgatct ctaaatctga aagttgcagc agattcttgt
    18661 tcgttgtttt ttctcttaat ttcagattaa gccgctgacg ttcactattc acttcctcga
    18721 ttaaaggaaa aaaggaatca gaaatcaaaa acaatgtacg aaacaataaa ctgtatacac
    18781 tcatgtctgg cattcgttcc aaaagcgatc gaattagtcg aatcacgtac tcggtattcg
    18841 gcgtggtaaa tgtaaacaaa ccgtcttctt ttaaaataaa agcgatagga ctagtttcat
    18901 aatgattatc gactttttgc tggtgcggaa catttgatac aagcagtaac gcttcatcaa
    18961 aagaatcgta ctctacgcgc gcccgttcat ttttatctag agaataagtc agcatctcgt
    19021 cactaatgcc atattcttct tttaagtttt taacagctag cacatcgttt tgctgtacat
    19081 tgatccaaaa attacggtct tgattaaaat agtgtttttc ccgcatactc tatcctcctg
    19141 acatttgttt ccagtataaa gaaaaaagtc gctcaaataa aggtttaccc tttatttgag
    19201 caactgtaaa tcaaatggct actttggttt ggcgaatctc tgctaatttt tcttcgatca
    19261 ttttcaagac ttcttctttt gcttgaggat cttccacaaa attatagcgg tctccatcga
    19321 tttggatttt tggactttct ttgtattctt cataccattg atcgtaacgt ctgttcaatt
    19381 ctttgtaata atcgtataga cttggatcaa aggacaattg ttcatacgaa cgaccacgtt
    19441 tttcgatacg ctccaacatc gtatcaaaag atacacgaat atgaactaat aaatccggat
    19501 gttttttatg ggcagcatag ggaagttctt ccatcatgtt ttccagcagt tcatcataca
    19561 cacgaacttc tgtttctgtt gctcttccta gatctgcatt tagatggaaa agcagtgaat
    19621 cttcataaat cgaacggtcc aatacattat tgtcatcttt cattgcttgt ttgatgcttg
    19681 caaaacgttt attcaaaaaa taaatttgca gcaaaaaagc atactgttca gggtttgcat
    19741 aaaataaggg caagacttca ttatcatcaa tcgattcata aaatgcctga ctatcaaagt
    19801 gttcagccat catttctgtt aaacttgatt ttccggcacc aattgtacct gctaaaacga
    19861 tcacgctcat ttacctccag ctcacttcat cactaaatat tgtgtttaac tccaaccttt
    19921 ttaaatatac ccccaatatc tagtagtgtc aattgtattt ttttctagtc aaagaagaaa
    19981 aaaagtcaaa aaattcttaa gaaaaaagtg ttttttcttt agaaaacgta ttcttagttt
    20041 tacttctgag atggttttag taaaataaag gaggaatgtt ttttgagacc cttagtggac
    20101 aaaaaaattc gtgaagcagc gcaactcgtt ttgggcgctt tattttttgc ttaaaaatca
    20161 cttttacgtg agaaggagtt agattatggc aaactatggc caaggagaat caagcggaaa
    20221 gatcatattg atgggcgagc acgcggttgt ttatggagaa ccagcgattg cctttccttt
    20281 ctatgcaaca aaagtcaccg cattccttga agagctggat gcaatggacg atcaactggt
    20341 ttcttcctac tattcaggaa atttagccga agctcctcat gcattaaaaa atatcaaaaa
    20401 attattcatt cacttaaaaa aacagcatga catccaaaaa aacttgcaac tgaccattga
    20461 aagcacgatt cctgctgaac gtggaatggg atcaagcgct gcagtcgcca cagcagtcac
    20521 tcgtgctttt tatgattact tagcatttcc tttgtctcgt gaaatactat tagaaaatgt
    20581 ccagctttcg gaaaaaatcg cccatggtaa tcctagtgga atcgatgcag ccgctactag
    20641 cagcttgcag ccgatttatt ttacaaaagg gcatcctttc gactactttt ctttgaacat
    20701 cgatgctttt ttgattgtcg ctgatacagg aatcaaagga caaacaagag aagccgtcaa
    20761 agatgtcgct cacctctttg aaacccagcc tcatgaaact ggacaaatga tccaaaaatt
    20821 aggatacttg acgaagcaag caaaacaagc aatcattgaa aattcaccag aaacgttggc
    20881 acagacaatg gatgaatcac aatcacttct ggaaaagctg acaatcagca atgattttct
    20941 taatctattg atccaaacag caaaagatac cggggcattg ggtgccaaat taactggcgg
    21001 tggacgcggt ggctgtatga ttgcattagc acaaacaaaa acaaaagccc aagaaatcag
    21061 tcaagcactt gaagatgcgg gtgctgctga aacttggata caaggattag gagtacatac
    21121 ctatgtttaa aggcaaagca cgcgcatata ccaacattgc tctaatcaaa tattggggta
    21181 agaaaaatga agaactcatc cttccaatga acaacagcct ttcgttaaca ttggatgcat
    21241 tttatacaga gacagaagtc atcttttctg atagctatat ggtagatgaa ttttatctag
    21301 atggcacctt gcaagacgaa aaagcaacaa aaaaagtcag tcagtttctt gacctttttc
    21361 gtaaagaagc cggcctctca ctaaaagctt cagtaatcag tcagaatttc gttcctaccg
    21421 cagctggatt agcctcctct gccagcgggc tagctgcttt agcaggcgct tgcaatactg
    21481 ctcttaagct tggattagat gatctctctc tttcaagatt tgctcgacgc gggtctggtt
    21541 cagcttgccg aagtattttc ggtggtttcg tcgaatggga aaaaggccat gacgacttaa
    21601 gttcttacgc taagccagtc ccttccgatt ctttcgaaga cgatttagca atggttttcg
    21661 ttttgatcaa cgaccagaaa aaagaagtgt ccagcagaaa tgggatgcgt cggacagtcg
    21721 aaacatccag tttttatcaa ggctggttag attccgttga aggggatcta tatcaattga
    21781 aacaagcaat caaaacaaaa gatttccaac ttctcggaga aacgatggaa agaaacggac
    21841 taaaaatgca tggaacgact ttagctgccc agcctccatt cacttactgg tctcctgaca
    21901 gcttaaaagc gatggatgcg gtgcgacaat tgagaaagca aggcattcca tgctacttta
    21961 cgatggatgc tggccctaat gtcaaagttt tagtggaaaa cagccattta tctgaggtac
    22021 aagaaacatt taccaaatta tttagcaaag aacaagtaat tactgcacat gctggtccag
    22081 gaattgcgat tattgaataa agggagattt ttatgattga agtatctgca ccaggcaaac
    22141 tctatattgc cggagaatat gcagttgttg aaacaggcca tccagcagtt atcgctgcag
    22201 tcgatcaatt cgtgacagtc actgtagaat ctgcacgaaa agtcggaagt atccaatctg
    22261 cacaatatag tgggatgcct gtacgttgga caagacgcaa cggagaatta gttttagata
    22321 ttcgggaaaa tccttttcat tatatccttg ctgctattcg cttgactgaa aagtatgcac
    22381 aagaaaaaaa cattctttta tccttttatg atctgaaagt aacaagtgag ttagacagtt
    22441 caaatggccg gaaatatgga ttgggttcta gcggtgccgt gaccgttgca acggtcaaag
    22501 ccttgaatgt tttttacgcg ttgaatttat ctcagttgga gattttcaag attgcagcac
    22561 tagccaattt agcagttcaa gataatggtt cctgcggcga catcgctgct agctgttatg
    22621 gtggctggat cgctttctca accttcgacc atccttggct ccaagaacaa gaaactcagc
    22681 attctatcag tgagttactt gccctggatt ggccaggtct atccattgag ccattgattg
    22741 ctcctgaaga tttacgttta ttgattggtt ggacgggtag ccctgcctct acttctgatt
    22801 tggtcgatca agttcaccgt tcgagagaag ataaaatggt ggcttatcag cttttcttaa
    22861 aaaatagtac agaatgtgtc aacgaaatga tcaaagggtt taaagaaaat aatgtaacgt
    22921 tgatccaaca gatgattcga aaaaaccgac aattactgca tgatttatct gcaatcactg
    22981 gggtcgtcat cgaaacgcct gctttgaaca aattgtgtaa tttagctgaa cagtatgaag
    23041 gagccgcaaa atcttctggt gcaggtgggg gcgattgcgg aatcgtaatt gttgaccaga
    23101 aatctggcat tcttccttta atgagtgcat gggaaaaagc agaaatcact ccactgccgt
    23161 tacatgtcta tagcgatcaa agaaaggaaa accgatgaat cgaaaagacg aacatgtttc
    23221 actagctaaa gcctttcatg ataaacaaaa aaacgaattt gacttcgtac ggatcatcca
    23281 taaccctttg ccgcaaatag cagtttctga tgtcgatcta agtacacagg cagttgggtt
    23341 tacactaagc agcccatttt atatcaatgc gatgactggc ggaagcgaaa aaacaaaaaa
    23401 aatcaaccaa gatcttgcaa tcgtcgcaag agaagcagat ttgatgattg ccactggttc
    23461 agtcagcgct gccttgaaag tcccttcttt agccgatact tatacgatta tgcgacaaga
    23521 atatcctcac gggaaaatca ttgccaacat cggtgcaggg acttctgtcg aaagagccaa
    23581 agaagctatc cgattgtttc acgcagatgc cttacagatt catttgaatg cacctcaaga
    23641 gttggttatg cctgagggag accgtgattt taccaattgg aaagtactta ttcaagaaac
    23701 tcaaacagcc attgacgtcc ccctcatcgt aaaagaagtc ggttttggca tgacaagaga
    23761 aacgctcaat gatctagctg ctttaggagt tcatacagtt gatatcagcg gtcgaagcgg
    23821 aacaagcttc acccagatcg aaaacgcccg tcgctccaag cgggaactga gttatttagc
    23881 tgactggggg caatcgacag tctcttcatt gcttgaagca aatgaagcag acacttctat
    23941 ggaaatccta gcctccggag gtatccgcaa tgcctatgac atatttaaag ccctttgtct
    24001 cggcgcaaat gccgtgggta cttcaggaac cgtactgacg catctgatga atcacggcgt
    24061 agaagaaact attatactga tgaagcaatg gcaagaagag cttcgtctgc tttatactat
    24121 ggtcggcgca acgaataccg cggccctaca ccaacaatct ctgatctttt ccggaccagt
    24181 gaaggattgg tgcgaagcaa gagggatcga cttagtgaag tatggaagac gcacagaaaa
    24241 gagcgtggga caaaagtaaa gaacactttt gtctcacgct ctaaacacgt acaaacggcg
    24301 gaagtaaaag caactccttc ggaaataagc tgaaattcac aaaaatttga aaagcaattt
    24361 tcgtgaattc cttcttattt cttggagtta aatgcttctg tcccagcctc ttttttcgaa
    24421 taaatgatgt ttaatcagct gtccttcggt attcgttcaa tccgctgatt tcacaacctc
    24481 tccactaaac agtgtactaa agctctttta aggttgtttt ccgatatagg caagtatgcc
    24541 tccgtccaca tacaagacat ggccgttaac gaaatcagat gcgctagaag ccaagaagac
    24601 agatggtcca gccaaatcga ttggctcgcc ccagcgtgct gctggcgtac gtccaacgat
    24661 aaattgatcg aatggatgac gttcaccgtt ttcttgtgtt tcgcgtaatg gagcagtttg
    24721 cggtgtagca atataacctg gtccaattcc attacattgg atattgtatt gaccgtattc
    24781 agaagcgatg tttttcgtca gcatttttaa tccgcctttt gccgcagcat aagcactgac
    24841 cgtttcgcgt cctaattcac tcatcatgga gcagatattg atgattttac cgccgccttt
    24901 ttcgatcata tccggaataa ctgcttttga aacgatgaat ggggcattta aatccacgtc
    24961 gattacttgg cggaagtctg ccgcatccat ttcgatcatc ggtgtccgtt tgatgatccc
    25021 tgcattgttg actagaatat caactgaacc tacatcttct ttgatctgtt gaaccatcgc
    25081 ttgaaccgct tcttcatcgg tcacatcgca gacataacct tttgcatcga tgcctgcttc
    25141 tttataatgc gctatacctt catcaactga ttcttgattc aagttattaa atacgatttt
    25201 tgctcctgct gtagctaaag agcgtgcaat ctcaaatcca ataccataga cagcaccggt
    25261 gactaatgct acttttccat ctaaacgaaa catatccata ctaaattcca tcattttctc
    25321 tcccttatgc ttattttaat tgatccattt gcaccatatc catgtctgta taggtgatat
    25381 tttctccgca cattgcccag atgaatgagt aattgcttgt acctacacct gaatggatcg
    25441 accagcttgg cgaaataatg gcttgttcat tgtctacaac taaatgtttt gtttcatctg
    25501 gtttgcccat catgtggaac acacgtgtat cttcattggc ataatcaaag tagacataag
    25561 cttccatacg acgttcatgt gtatgacaag gcatcgtatt ccaagcgctt cctggttcta
    25621 aaatcgtata acccatttgt aattgacagc tttcgcatac atttggatgg atgtattggt
    25681 agattttacg ttcattcagt gtcaggcctt caccagtctc cattggtttt acttggtcga
    25741 tactgatttt tacatttgga tatttatgat gagctggaac agaactaata tagaatttag
    25801 ctgggttttc agcatcttga gaagaaaata caacatgtcg tgtttctttt ccgatatagt
    25861 atccgtcttg ttttttcatt tcttcttttt gcccatcgat ctcgatgaat cctggtccac
    25921 cgatattgat cacacctagt tcacgacgct ctaagaagta atcaacacct agttctttgt
    25981 ctaatgtgat ttctaattct ttttcagtag gtgtcactcc accaaagatc attcgatcgt
    26041 tgtgcgtata agtcaaactg attttccctg gttcaaatac cttttctact aaaaattctt
    26101 ttctcaattg ttccgttgaa taatgacgga tatcttctgg actgtgtgta taacgtgttt
    26161 ccatttcttg cattttcttc tactccttct atcgaatgat ttttccagag ccgcatgcta
    26221 aaaattgtgc gacttcttct tgactaaatt ggttgcagtc cccatggatc gtatgcttca
    26281 aggcagaagc tgccgtagca aaatcaatga tttcttgcgg agacatattg gataaaatcc
    26341 catggagcac gccacctgca aaagcatctc cgccacctac tcgatccacg attggggtga
    26401 ttctatggac ttgggattcc acatattcat ggttcatcca caatgtcccc ttcaattcat
    26461 tttcacttgc tgaatgaacg gtacgcttgg tcgaataaaa cacctgaata ttcgggaact
    26521 tttcctgcat tttttgataa taataaatta gaggttgttc ttctgcttct ggcgctttct
    26581 cgatacctaa caaataaaga gcatccattt ttccagctga gcagatatct actaaaggta
    26641 aaagctgatg gattacttcg ctggcttctt gctgtgtcca taattttcca cgatagttga
    26701 tatcgaagct gatcaggcag ccagcttttt ttgcagcttc tatcacctgt ttcgtcattt
    26761 tttgccattt tttagacaaa gctggggtga ttcctgaaac atgaaatata tctattcctt
    26821 ggaataactc ctctagcgac cattccaaat gatccatttc agcaaaactt gatccggcac
    26881 gatcatagat cactgaggcg gcgcgttcgc cactaccagt ttccatataa taagtaccta
    26941 gtcttgatcc accagataat acctgtttcg tatctactcc ataacattgc aagtgctttt
    27001 tgactgcttt tcccaatgcg ttatcaggta cttttgtagc aaaaatcgct tcatgtccgt
    27061 aattagctaa agaaattgct acgtttgctt cccctccgcc ataatgggca gcgaatcgtt
    27121 cacttgaatc gatacgggtt ccctgctctg tggacaatcg caacatgatt tctccgagtg
    27181 tcactacttt tcccataagc agctacctct tgatttcttt catttttgct gcatactgtt
    27241 gcgcgacttc cgttactttg tcaaaatctc ctgtagctgc gggtgctaaa aggttcccac
    27301 ctactccgac tgttacgaca ccagcatcga accactcttt catattgtcc agactcacac
    27361 cgccagttgg catgatattg acttgaggca tcggcgcctt gaaggcagaa atgatacttg
    27421 gtccataagc gcttccagga aatagtttga cgatatctac cccgcttttc agcgctgttt
    27481 tgatctcggt aatagtcata catcctggta agtaaggaat ttgatacaga ttgcacatct
    27541 ctgccgtttc ttgatcaaag gaagggctaa caatatactc tgcgccagct aagatagcca
    27601 agcgagctgt gacagcgtct aatactgtgc ctgcgccaat cactatttct ggttggtctt
    27661 tgtagaatga caaaagttct ttgattactt gatctgcttg agggactgta aaagtcaact
    27721 caataccagt tagaccacct ttgatgattg cgtggcaagc attcaaagct tcttctttgc
    27781 tttttccgcg taccaccgca atcactccgg cgtttttcaa tcgagataaa atatcaattt
    27841 ttttcatctt ctccacctat ttcctattta gaaattttat tttataactt ggaaatctat
    27901 acccatatta tacggcagaa aagaaaaatt gcaaacgctt ttttcattat ttttgaattc
    27961 tttgctcgat ttcgcttttt gtttgcttga gggcactgat tatttgttct tttgtctcat
    28021 ctgttaaacg atatttcggc atacttacac taaatgcacc aatgatttgt tcgtttttca
    28081 cgattgaggc tcctatacaa aaaatatcac gttcgatttc ttcatcgtca aatgcgatgt
    28141 gggtttcttt taccgttttg atttcctttt tcaaccgtaa tgcattggtg atcgtatatt
    28201 ctgtgtaagg gatcaacgga tgcttttcaa gataatcagt gtactgctct tcactgaatt
    28261 ctgctaatac ggctttgccc atcgcagaat tgtataatgg tcgggtgatt ccgatttttg
    28321 aagacatgcg gatcgtttga tgttttggat ctagtttgtt cacgtacaag atctcattgt
    28381 tgtccaatac ccctaagtgg atagtctcat cgatcttttg ctgcagctgt tctaaaaacg
    28441 gtaaagtcaa ctctaccaaa tcgatttgtt caatgttttt attcgcgtag cgaataagct
    28501 ttgcacctaa acgatagttt ttctctgaat ttttatttac gtagccgatc atcaacaaag
    28561 tatctagtat tttcaagact gtagaagagg tcattcccac accctttgcg atcgtctgca
    28621 aagagacatc ttggttttcc gctaaatagt ctagtatttt agaggctttg atcaatacag
    28681 ttccataagg ctttttattt tccatataat attctcctat ttctttataa gaaaaattat
    28741 agcggaaata ccatcaattt aaaagagata tccagatata aactaccatt tctctttaca
    28801 ggcaatttaa tgttgaagat ccgcttcgtt tttacatcaa actttgatat aaagcaatga
    28861 tttcttctac tgtcgtttct cttggatttc ctggtgtgca tacatctcgc aaagcatctt
    28921 ctgcaattgc tggaatatct gcttctttta cacctaattc agaaatggtt tctgggattc
    28981 caaccgcttt gctcaaccga tcgatctcac cacaagccgc ttctcttaca tcttctaaag
    29041 acatttcagc tgctccttta atccctagaa caagagcgat ctcacggtac ttttctccag
    29101 tatattcttt gttatatttc atgacagttg gcaaaagtgc tgcacacgct acgccatgag
    29161 gaatgttgta ccatgcactt aacgggtgag ccatcccgtg taccagtcct aaaccgacat
    29221 ttgagaagcc cattcccgca atatactgtc ctaatgccat tttttctcgc ccattttgat
    29281 cgccatctac agaagcttcc aaagaatggc cgatgatttc gattgctttt aaatgcagca
    29341 tatcagtcat ttcccaagcg ccttttgtga taaacccttc aatagcatgc gtcatagcat
    29401 ccatacctgt tgatgcagcc aattttttcg gcattcccat catcatatcg ctgtcgatga
    29461 acgcgacgat tgggatatca tgaggatcaa cacaaacaaa ttttcggtga tttacttcat
    29521 ctgtgatgac gtagttgatc gttacttctg ctgctgttcc agaagtcgtt ggtatagcta
    29581 atataggtaa acatggattt ttcgtatcag caacaccttc taaactaatg acgtctgaaa
    29641 actctggatt cgtcaaaata atcccaatgg cttttgccgt atcgattggc gaacctccac
    29701 cgattgcgat caaacagtct gcctctgcct ctttaacaaa agccactcca tcttttacat
    29761 tttggatcgt tggattaggt acgatcccgt cgtataaagc ataagcgatt gctgctttat
    29821 ctagaagttc tgttactttt gttgctatac cgtgctcgat caatcctttg tcagtaatga
    29881 cagctgcttt gccgaatcct cgctgctgaa attcagggac gatcgactca atcgctcctt
    29941 ttccaaaata cgatgtttca tttaaaatca ttcgattact cattttctat tcctcctaat
    30001 ttgtattatt tttcacaata aaaacgaaag aaccccaagc aaccggttgc ccagttgctt
    30061 gttgctacag ttcaaagact ggcatcaatt cccaactgac tggagaggca tctggatttg
    30121 tctccatgat atcttccatg tacttccacc atttttgatt gatagatgta tttgccattt
    30181 tttcccataa cttttcatct tcgatcacta agtaaccaaa taacaaactt gtactcggat
    30241 caagatagat cgaatagtcg atgaccccgt gttcacgaag tgctgtacgc atttctggcc
    30301 aaagttcatc atgtcttttt ttgtattctt cttgtttttc tgggtaaact ttcatgcaaa
    30361 aagctttttt caccatgtca gacacctgct ttcgtatgaa cttcctccaa ataacctgct
    30421 tttggagtga caccaaacgc atctgctaaa tgccacaggt tttcgtccgt gattgtttgc
    30481 aagattggtc cttgcgcttt gacgtacgta tagacttctg ctgccttttc tgctgtctcg
    30541 atcaaaccaa agacttcatc catgtctttt cctgacccgt agattccatg ttgtggccat
    30601 aaaaccaatc gagtttcttt cattttttca gctgttgctt ccccaatctc atttgttccc
    30661 ggaaccatcc acggaatgat ggaaattcct tctggaaaaa caactaagca ttccgtacac
    30721 atttgccata gcgtacgact aaaggaacgt tcgtctaatt catgcgtaaa actcatcgct
    30781 aatagatgac tagcatggca atgcataacg atccgattat ctgggtctac ttctaaacgg
    30841 gcgatatgac tcatgaaatg acttggcaac tcgcttgtag gaaggcattc gttttccagc
    30901 ccccacaaaa gttccagtgt tttgccatct tcagcaactc gaacgattcc aagattctcg
    30961 gcaggttcgc tcgctacatt tttaaaatat cttcctgaac cagttacaat aaaatagcat
    31021 cctgctaaac ggctggcatc aaaaatcatc gagatgcggc ggatcacttt ttcaggatcc
    31081 aagaacggtg tgatctcttc ttcttttagt aaataagaaa tattcccgcc atttctttca
    31141 tcccatccca agcgataaag attcgacgtc gtttcgatca tttctttcac ataaggcgct
    31201 tgtaaaatat ctattttttt caatatagcc acactcctat tttcttaatt ccaacacgtc
    31261 tttttgatat tcctggactt cttccagcca atccaaacca actggaactt gatttttgta
    31321 acagaaataa ttccatacat cggcatacgg gaaatctttc aattcttccg tataagccat
    31381 tcttgaagta aagtcaaaag ccagctccat ttctttcaat cgctcaactg gttccagcat
    31441 agctttcagc agtgcttttt gtgtatttct cgtaccgatg acccaagcag cgatacggtt
    31501 gatcgttgca tcaaagaaat ccagaccgat atttgttttt tccaacaatt cgttacggac
    31561 taattcttta gcgatttcct gcaattcatc atccatcaaa accacatggt ctgaatccca
    31621 acgcactgga cgggaaacat gcaacatgat tcctttgctg aataaagcta atgaagataa
    31681 tttattggaa atcacttctg ttggatgaaa atgtccagca tctagacaaa caagtttgtc
    31741 acgagtcaac ccgtaaccca tataaaattc atgggaaccg actgtataag cttcagcgcc
    31801 taaaccaaat agtttgcttt ctactgcatc ttgtacatac cgttcatcta actcttctgt
    31861 aaaaatttca tcgagtgatt ccattaaacg ttcccgtggg gtaaaacgat caatcggatt
    31921 atctttcatc ccatcaggta cccaaaagtt attgatactt gtttgcccag tttctttccc
    31981 aaagtactcc gcgattttgc ggcttctttt cccgtgttcg atccagtatt cacgaacttc
    32041 tttatctgga tgagccaatg taaagccgtc tttcatcatt ggatgcgaaa agaaagtcgg
    32101 attgaagtca agaccgattt cttctttttt tgcccactct acccattttt caaaatgttt
    32161 cggctcaatt tcatttagat cgacagattc ttctgtatcc aaatagatag catgaaggtt
    32221 caatttgtgt ttgcccggta tcaacgaata agccttctcc agatcttgtc taagctcttc
    32281 aggtgttcgt gcggctcctg gaaaattccc tgtcgccatg attccaccgg tcaattcgcc
    32341 atccggtgtc aagaaacctt tgacatcatc tccttgccag caatgcatag aaattttgat
    32401 gtcctgtatc ttttctagta cagcatctgt atctacaccg atagttgcat atttttcttt
    32461 tgcttgttcg taatttcttt caatcgtgtt catacgatta ctcctttgat tttattcacg
    32521 taccattcca tcgtgttttg cttattttca tctgggtaaa aagtttcaca tggaaacgac
    32581 tgtttgatta catttcttgc ttgttctata ttcgaaaact gacctgtact gagcatttgc
    32641 atggcaacat tcccgattgc tgtggcttca ctaggtcctg cttctacaat gattccggca
    32701 taatctgctg tcaattgatt cagtagacga ttattcgatc cgccccctac gatgtgcagc
    32761 ttttccaatt tcgttccagt taagtgttct actttgatca gctcttgtgc gtaacaaaga
    32821 gcaagattat catacacaca acgagcgata gctcccgatg agacagggac tggctgctct
    32881 gtttcaaaac agtattgctg aatctcttct atcatatttg ggggattcaa aaatcgaggg
    32941 tcattgattt ccatcacttg ctgcagcggt ttttcttttg cagcaagctc tgccaattcc
    33001 gcataactgt actcgtactc ttgcattcgg gcaacttcct ggatcaacca catccccatg
    33061 atgtttttca aaaaacgcac cgtgttatgg gcaccccatt cattcgtata attttcagaa
    33121 aaagcattgc gagaaacatt ggcgatcttt gtttcaatcc ctaaaagcga ccatgtccca
    33181 ctgctgatat aaccccaacg ttctccttga cctggcgttc ctaatacagc tgacgccgta
    33241 tcatgacttg ctactgtgat aaaggtcaca tctggcaaat catattttga aaattgttct
    33301 ttttgcagat ctcctagtat cgttcccggc tctgtcaatg gtggaaataa ttccttcgag
    33361 acaccgattt tttcaagaag ttctatttcc cattcttttg tccctgcatt cagcagctgc
    33421 atagtagatg cattagtttt ctccgtcacc atgttaccag taaagacata ccctaaataa
    33481 tcaggtatca gtaacaattt atgtgctttt gccaaatttt ctttttcttc agcaaacaat
    33541 tggaacaagg tattgaaagg ctggatctga atccctgtct gctcatacaa cttatctaga
    33601 gaatagattt cagaaaactg ttctactgct ccattggtac gcttgtcccg ataagcaatc
    33661 ggatcattta tccgatcccc ttgttcatca atcagacagt aatcgactgc ccatgtatcg
    33721 atccccgcaa aaaggcgatc aatccctgtc tgctttaatt tttcaaatcc aatcaaaatc
    33781 tctttgataa gataatcgat cttccagcga tcgtgaccgt ctttgttccg aaagccgttt
    33841 ttgaaccggt gtacttcttc caagatcaaa gaaccttttg catccctcgt acttttcatt
    33901 agtcgtccgc ttgatgcacc gatatcgatg gcaacaaatg ttggattcat ttctccacct
    33961 cttttacttt attgttgatt agcactttat taataaagcg ttatcattta ttgacaatgc
    34021 tatcataata cagaatctta tattttgctt cacactattt tggtatataa ttgcattatc
    34081 ttgacaagtt gggagtgttt acatggatta ctgggaaaaa gccaaccact catggtcgga
    34141 agactcaaaa agagtgatct tgacgcctac acaaagaagc aggaatttat ttttttatat
    34201 ccaagaaatc ggttacttca aagcaccaat cccttactat actgaacgag cacatttacc
    34261 ttcttacttg ataaagttta ctttgtctgg caaaggttcg cttacatata atgataatca
    34321 gcaacagcta aaagctggag acttattttt tattgactgc caagaatatc agcgttatca
    34381 aacgatcagc gaagaaccat gggaaatgga ttggatccat ttgaatggtc agaacgtcca
    34441 atcattttat caggagttca caaaagacgg ccataacacc ttccattcaa caggagatcc
    34501 atcagaaaac aagatccacc atttgatcac agaattgatc cacatgcaac aaggcacgaa
    34561 tgcccgtacc gatttccagt cctctgtagc tatccacgag ttgctgaatg aactgatttt
    34621 gcaaaagtat cagctagatt tcgaagaaga ggatattccc gcctacgttt acaaactgaa
    34681 gcaatactta gacgaacatt tcaaagaaac gattacactt gatcaattag aacaacaatt
    34741 cttattgaat aaatatcagc tgagcaaaga tttttccaaa tttatcggtt ctccaccaat
    34801 cgattatttg atcagcaaaa aactttctta cgctaaagac cttcttcggt atacagaatt
    34861 tacggttcga atgatttccg aagaaatagg gattgagaat tttgcttact ttagccgcct
    34921 gttcaaagca aaaacaggac tatcgccgag agactttcgt aaattcagct gattcatata
    34981 atatgtacgt cattttttca aaatattttg aaatatatag ataaaaataa aataaccgca
    35041 gtttctgctt ctatgtaaac aacagcagct actgcggtta ttttttagtg atcattctca
    35101 ttgttcatct tcaaaatgtt cataatgaac caactgtttc tgtgtatcac gtttcaaaaa
    35161 ttctttaggc gttatgccga actctctctt gaatgctttc agaaaatgcg gataagaaga
    35221 aaaaccactt ttactatata cagcactaga gctgcggtat tctcgcaaca agtgcttggc
    35281 atagatcagc tttttcttca ttacatactg atgaaacgta aatccagtgt actttttaaa
    35341 ttctcgtgtg atatggtatt tgctgacaaa aaactgcttt tccatttcct ctaaagaaag
    35401 cggatgagat aaattttttg agatatattg taacatttgt tccacacgct cattaggaac
    35461 tacagcattc tgttcttgcg cactctcttc tttttgtaca acttgattga gaaaaatcaa
    35521 gtattctacc agagcttgtt catattttat gtcttcaccg taaatttttt tatcaggagg
    35581 atggtcgata aatgataatt tttgcttcaa tacttcctgt tctatttgca atacacgact
    35641 cctcttctct cccattggtt gaaaacaagc cgatagatta gaccatggcg tggaacgact
    35701 ttctagaaaa gaaggggtga caaagatata gacacgctcg aatacatctg aagattggcg
    35761 gacgatacga tgcaaatcat tggaatggat gagcagcaca cttcctgctt tgatcgtaaa
    35821 ttgtcgtccg ttcaaataaa aagtcgcttc tccctttaat gtagcatgga tctcatagaa
    35881 atcatgatga tgatagatcg ttttattttg gctgacgaca tctttgacat gatagatttc
    35941 atactgatca gaaaccatcg tattgacttg ttcaccgctg attttctttt ggagcatgtg
    36001 ccgcacctcc ttttgttcac ttatgcacaa ttttattata ccacgttttt tgtaatcaaa
    36061 aagagctgct tttatatact cttataaaaa agaagagacg tggcatcttc tagccccgcc
    36121 ccctccattc taaatattag ttaaccaacg tcttctttac attattcaag aagacttctt
    36181 ccataacgaa tcccatcatc ttttgtttct tcaagaacca catacaggta cggacgattc
    36241 ttcatgatga tttctttgat ttttgtgtac tcgatcacac cgtctcccaa atttaccggc
    36301 acgatttttt cctgttcgac acgaaaatct ttcaaatgaa ccgcacatat cttttcacca
    36361 aaacgttcaa aagcttcttc taccaattgg acttgttctt gataatcact acttgtgatc
    36421 aaattggtcg gatctaaaat gattcctaga tagtccgaat ctactgcttg gatcagctgc
    36481 tctacccttg ctaaagaata caccggatga ttcaaacctg gttctatccc caccatcatt
    36541 tggtattttt ctccagcttt taccaagcga cggataacgg acacagcttc agcaaatgct
    36601 tcatctgtga aattttcttc cgtatattgg atttccggca aaacacatcc agtttccgaa
    36661 gcaaccatcg acgcaccaaa ggaagaagca tagcgaagat actgctcgaa ctttgtcagc
    36721 aactcttgtc gtatcgttaa atctggatga atcatattga tatagcaact taaaattcct
    36781 acggtcactt cttgcttttc taaaactcgt ttgacataat ttcccatccc agaattgatc
    36841 tcatttgcgc cagaaggtat ttcaggaaac gaaagaccta aggcgagctg cagtgtatga
    36901 atcccttgtt cttttgtttt ttctgcaaga tcttctatcg tttggacttc ggaaagatca
    36961 tgtcctctaa ttgtaatgtt caacataact attctcctaa ctcagcatat tgggtagcca
    37021 catgacgatt tgcgggaagt aagtaatcaa aaacaagacg actaagataa ccccataaaa
    37081 aggaagaagc ggtttcagca ttgcctctgc ttttacgcct cctacacttg caccgacata
    37141 tagtcctgta cctactggcg gcgtgatgga gcctacacac agattcatga ttgaaaacaa
    37201 cccgtaatgt acaggatcaa cccctacact tgtgacgatt ggtaaaaata ttggtgtaaa
    37261 aatcaagata gcaggcgcca catccataaa catacccacc agtaataaca caagattgac
    37321 taataacaga ataataaatt tattatccgt tacgcccata atcaacgaac taattgcctg
    37381 aggaataccg gtaaacgcca tggcgaaaga catcatggaa gaagtagcca ataagaacat
    37441 gatcgtccct gacattgcta ctgattgaaa caacatatta ggtatatctt ttagttttac
    37501 cgttttatag taaaacatag caatcaacag ggagtaaatg acagctatcg ctgaagcttc
    37561 tactgctgta aatatccccg tcaagatacc accgataatg atgacaatca atagaaggct
    37621 aggaatcgcc tcttttatta tttttccagc atgagccatc tgtccttttt cgacaacagg
    37681 gtatttattc tttttagcaa cgatgaatgc tactagcatg attcctagcg cccacaaagc
    37741 gcctacgaga taacctccca taaacaaagc agagatcgaa gcaccaccag caacaagtga
    37801 atacatgata aaagcagtac ttggaggaat gaccatacct gtcggagctg acgcgatatt
    37861 gaccgctgtc gcaaacttcc ggtcatatcc ttcttccact tgctgaggga tcaacactcc
    37921 gccaatcgcg gtagaagcgg caatggccga gctagagatg gaaccaaata aagcattccc
    37981 taacacattc gtatgagcca aagcccccgg cacgcgcccg acgaacaaca tcgcaaaatc
    38041 cactagtttc ttagctatcc ctccattatt catgataatg cccgagagaa cgaaaaacgg
    38101 aatagcgatc agcgaaaaac tattgatact tcccaccatt ttttgagctg aactaaaagc
    38161 agaaacatca aacggtataa ctaacaataa agtcatcatc gatgagaatg caatacttat
    38221 agcaatcggc atccctaata caagcaacac aacaaataca aaaaataaca ctaatccggc
    38281 ttctattgcc attttattcc tccattttta tattttttct tgaattgctg gcttgttgct
    38341 taatgtatcg attagattga tcaggctata gataatgatg agtacccccg atacaggtac
    38401 tgacagataa acatatccca taggaatact taaagaaggc gagatctgcc ccattgtcaa
    38461 tgtgacagca ttccatccgc catacaccat gatcatcgca gcaaatacca gaaataaact
    38521 ttgaacaaca acttctaaaa ttttactttt tttgccagtt aatttttcac ttaaaaccgt
    38581 tacggctaaa tgtgcttttt gtcccacaac atacgcggaa gacaacatgg caaaccagac
    38641 aagaccgaat cgaacaagct cttccgtgac cgtatttgga tttcctaaaa ccgttctgga
    38701 aaatacttgc cataaaacaa cagcgaccat cccaacaagc aaaacagctc ccaggatctc
    38761 taataccttg tttaaaatca ttttcatcac agctacgctc cttgctcttg gatcttttga
    38821 taaaggtctt tgtaggtagg atcgtttttg aatatatcat gaagtggggc aactgcctct
    38881 cgaaacggtg tattatctac atcattgaat tggactccat gttcttcttg tgctaaagct
    38941 ttttcttctt caattgcttt tgcaaaagct tcaatttcaa aaaccgttga ttctttcgct
    39001 gcttcttcaa tggctttttc ctgctcatca gttagctgtt ctttgatggc atcattcatg
    39061 atgatgatat ctggcacacg tgtatgctca tcataggaat agtatttagc cacaccacca
    39121 tgtccagctg tatacaagac aaattcattg ttttctgatc cgttgatcaa attggattga
    39181 agagacgtgt acacttcatc acttcccata ggtacgggcg aaccgcctaa aagctctacc
    39241 attttgatag cggtttcgct ttgcatcaca cggatcttct ttcccttcaa atcttccggc
    39301 gtatggatcg gaccgtctac catataaaag ctgcgctgac cagaatcata ataggtcaat
    39361 ccggtaaatc ctaaattttt ggtcgattgg tagattggct ccattatttc ttcattatcc
    39421 atcaccttaa agaaatgttc ttcatcattg aatagataag gaactgaaaa aatcgaataa
    39481 tctttagaga aactttccaa cgctgaggca cttacttttg tgaaatctac tgctcctgac
    39541 tgtgtcaatt cgatcagttc acgttctcca cccagttgtc catctgggaa atactggatc
    39601 tgtacggaac catctgtttt ttcttttact aaacgcccaa attcattcat tgcatcaatt
    39661 actggctggc tgttgcttgc ataagcaaag cgcaaggtaa ctatctcatt tacatctgca
    39721 tctgcgacag ttgccacact agaattattt ccgcagccgc ttagacctag cagacctagg
    39781 ctaagcgaac ttgctgcaaa cagcagtgat gtttttttca tatgggtttc ctttctttta
    39841 acaataagag acgagcaatt ctgtcaaaca aagcaccgtt aacgactgtc cataaggcat
    39901 tgctgtgatt ccgatattcc gataaaaatc taaactgtcc cccattcctg tcccgattga
    39961 aacgttttga acttctcctt tttcatcgat ttgttcaagc aatccttgga tagcacgata
    40021 agcgacttct ttatattctt gtggtaaata acgtttatgg accgctttca aaataccgta
    40081 agcaaatcct gcagttgcag aagattctac gtaagaagaa ggatcatcca acaacgtatg
    40141 ccataaaccg ctttcagact gataagtttt taatgcacgc acttgtgctt caagtgtttc
    40201 gatcaataat ttgcgcaatg catcttcttt cgataattct aagatttcaa tgatctctgg
    40261 aatagcaatc gtcaaccagc agtttccacg agcccaaaat gcttcggcat agttatgatt
    40321 tccttcaaac gtccagccgt gataccacaa tcctgttcgt ttatccatca agtatttgat
    40381 atggatcata aactggtaac gagcttcttc taaatattct gggcgattca acagtttccc
    40441 gatttttgcc aaaggaagca ctgtcatcat caatgtatcg tcccacaact gattcttatt
    40501 ttccggtcca tatgtggcat gctgtaaccc gtcttcattt gtccgaggca tgtcattcat
    40561 gacccattct gcccattgtt ctaaataagg caaatagcgt gaatctttcg tgtcttcata
    40621 caagtaagcc atagtcaata gcggtgccat tgtattgacg tttttgggcg gagcgccttc
    40681 ttccatacga tcctcaaacc attcattgac cacttgataa gcgcgaggat tttttgtcat
    40741 ctggtaattt ttataaatac catatagacc gacaccttgt ggccaattcc acactgtcca
    40801 gcttttgtca tctactttca gtccatcaaa gtccaacaaa aattcaccat tatcgtcttt
    40861 gatcgttgtc aggttttcaa cgagcaggtc aattttttct ttcactaatt ctttaggtac
    40921 aaaaatctct tctttaataa tcatctttac gccacctttt taaacgcttt cattttatat
    40981 ccttaatata gcagtaatac cgtccactat aaatatcgct ttttgaacaa aaactgtcta
    41041 aaattgacct aaatgttaaa taacaaaagt aactttcagt tacttggaaa ttaaaaaaag
    41101 aacttggaga aacaacgttt tccaagttct ttttcatcta ttttcttatc ttttttctac
    41161 gctttctttt gcttatccgt tgtaagcatc aatgatgatc tgtttcaatt cagagatcaa
    41221 tggttctttc ggattagctg tcgtacattg gtcttcgtaa gctaattcag ccatacggtc
    41281 tacggttgta tctagtgttt cttgtgtgac tccttgagct tttagactca tgttgatgcc
    41341 gacagatttt cctaattcat aaacagccgt tgctaaagct tcgactaatt cctctgttgt
    41401 ttcgcctttc agacctaaga attttgcgat atctgcgtaa tctgtatctg cacggaaata
    41461 gtcatattta gggaacatcg catgtttttg tggatctttt gcattatagc ggataatatg
    41521 cggtaaaaga atcgcatttg tccgtccatg agggatcccg tattccccgc cgattttatg
    41581 tgcgactgag tgacaaatac ctaggaatgc gttagcaaac gccataccag ccattgtaga
    41641 agcgttgtgc attttttcac gtgattccat atcaggtgtt tttactgatt tttctaaata
    41701 gtcaaagact aacttgattg cttgtaggct caatccacgt gtataatctg aagccatgac
    41761 tgaaacatag gattcgattg catgtgttaa cacatccatc ccggtatctg ctgtgacaga
    41821 tgctggaact gacatgacga attgaggatc gacgattgcg acatctggcg tcaatgcata
    41881 atctgctaac ggatatttca catgtgtttc actgtcagtg atgaccgcaa atggcgttac
    41941 ttcagaacca gtccctgatg ttgtcgggat acatacgaat tgtgtttttt ctggtttgtc
    42001 gatcttatat gtccgtttac ggatatccaa gaatttttgt tttgcaccaa agaaagatgt
    42061 gtctgggtgt tcatagaaca tccacattcc cttagctgcg tccattgcag aaccgcctcc
    42121 aagagcgatt actgtatctg gcttgaagtc tgccatcagt ttcgttcctg cgtatacggt
    42181 atttgtcgat gggttcggtt caacatctga gaagacttcg atcttcacat catttttacg
    42241 tcgtgccaat acttcacgaa ctgtatcgca gtaaccgaat tggaccatac cagggtcaca
    42301 aacgatcatg acacgttcaa cgttttccat tttctctaag tacaatagag agttcttttc
    42361 aaagaagatt tttggcggta atttgaacca ttgcatgtta tttctccgtt tcgctacagt
    42421 tttgacattg attaagttta cagcagagac gttacgagaa acagagttct taccataaga
    42481 accgcatccc aaagtcaatg acggaatcat ttcgttatag atatcaccga tgccgccttc
    42541 tgcagatggt gtattcacta aaatacgaca agctttcatt cgaagaccga atttgacatg
    42601 caactcttcg ttttctgaat ggataactgc tgtatgtcct aatcctccaa gattcaacat
    42661 accttcacac aaatcaaacg catggtccgt gttgttagct ttgatcatgg caagaactgg
    42721 agaaagtttt tctcttgata gcggataatc tgggcctacg ccttccaatt cagcaatcag
    42781 cattttcgtt ccttttggta cactgattcc tgccaatttt gcgatttcca tcgcagaatg
    42841 tccaacgatt gatggattga ccgcatattt tccttcgttc atcactgcat cttctaattt
    42901 ttgcaattca tctggtttta cgatataaac ttggtgtgct tggaattctg cttttactgc
    42961 tgcatagatt tctttatcca cgatcacagc ttgttcagaa gcacaaatca ttccattatc
    43021 aaatgttttc gaaacgatca aatcgttcac tgcacgttta atttttgctg ttttttcaat
    43081 ataagctggt acgttacctg gacctacacc taaagctggt tttcctgtag agtaagcaga
    43141 cttgaccatt ccagctccac ctgtcgctaa gacgatcgcg atacctggat ggttcattag
    43201 catagaagtc gcttcgatcg aaggatgttc gatccattgg atacaatttt caggagctcc
    43261 ggcagcgata gcagcatcac gtaccactcg tgctgcttct gcagaagatt tttgggcact
    43321 tggatggaaa gcaaaaacga ttggatttct tgttttcaac gcgatcattg atttgaaaat
    43381 cgttgtggaa gtcggattcg ttgttggagt tactccgcaa accacaccga ctggttcagc
    43441 aatttcaatc aacccttttt ggacatcttc attgatcaca ccaactgttt tatcatgttt
    43501 gatattatgc caaatatatt cggacgcata catatttttg attgctttat cttcgtaaac
    43561 accgcggcct gtttcctcta cagccatctt cgctaatggc atatgttgat ccaaagctgc
    43621 cattgccatt tcatgcacga tatggtctac tttttcttga tcaaagcttt ccatttcttt
    43681 caatgctgca tttgcttttt ctgccaaatc atcaatcatt gcttgtacgt caattgtctt
    43741 cgttttttct ttttctatta ctttagccat cattatcctc ctaatgggtc atggttattt
    43801 gttaaataat tcacatgtat atcatacaat atttattttt ctatgtaaac acttttttgt
    43861 gatttttttc acagttattt ttaaatataa aattgcttta tttacaggtg tttgtataga
    43921 aaaatatata aagttattta ttattattta tatgacacac ccgattattc aatattgagc
    43981 aatatatccc atgctcttat ctctttcttt tttgaataaa aaatgaggta ggacatcttt
    44041 ctgccctacc tcgtctttaa ataagctttg atacttattt ttcttccttc tcttctgtct
    44101 cttccagcgt ttctgttgtt tcagttttta cttcaactgt ctcagctggt tcttctgttg
    44161 tttcaacagt agtcgttgca tcatttgtta cgacagtccc tggtttgacc gttttgattg
    44221 ctgcacgatc gtactcaagg aagatccctt cgcagtctaa aacaactgtc cgtttgtcat
    44281 tgtcgatttc tgaaacaact ccatgtaatc cgccgatcgt tacgactgaa tcacctggtt
    44341 tcatgctatc taacaagttt tgacgttctt gctgttgttt cttttgcgaa cgtgacataa
    44401 aaaaccacat tgctaatagc gcgataaaca tcacaagcat tggtaatgtt cccatttttt
    44461 tcacctcact aattctgtat ctacaaaaaa cactttagca gaaaaatgct gataacacta
    44521 gttttttact taatttttaa aagattttcc tttagaagct ttttgcgttt tctttattga
    44581 acccgtattc ttcaaaaaag gcttctctaa attcaagcaa attatcatcc atgattgctt
    44641 gacgtacttg tttcattaga ttcaacaaga agtataaatt gtggtaagaa gtcaaacgaa
    44701 tcccaaatgt ttcatcacat ttgatcaaat ggcggatata ggcacgtgta tagtttttac
    44761 atgtataaca atcacatttc tcatcaattg gacggaaatc atgggcatat ttcgcatttt
    44821 tcactactag acggccttga gaagtcatac atgtgccatt tcgagcgatt cgagtaggca
    44881 agacacagtc aaacatgtct actccacgga taacaccatc gatcaatgaa tcagcagcac
    44941 cgacacccat caagtaacgt ggtttgtttt cggggatcaa cggtgtagtg aattctaata
    45001 cacgattcat ttctgattta ggttccccga cagataaccc gccgattgag taaccaggga
    45061 aatccatccc gactagatct ttcgcgcttt gacgacgaag atcttcaaac cctgctcctt
    45121 ggatgattcc aaataatcct tgacggtctg gattagcatg agctttcaat cctcgttctg
    45181 cccatcgaga cgtacgttct actgattttt tcacgtagtc gtaactttca tcgaatggtg
    45241 gacattcgtc aaaactcatc atgatatcag agcccaattt attttggatg ttgattgctt
    45301 tttctggtga taaaaacata cgcgaaccgt tcaagtgatt tttgaaatgc acgccttctt
    45361 cttcgatatt ccgcatatca cttaatgaaa aaacttgaaa gccgcctgaa tctgttaaaa
    45421 tcggttgatc ccagttcata aatttatgca gtccgccagc ttcttctact aagtcttcac
    45481 cagggcgtag ccataaatga tatgtattac ttaaaataac gccagcaccc atttctttca
    45541 actcttctgg ggacattgtt tttactgtag ctaatgttcc tactggcatg aacattggtg
    45601 tggggaaagt tccgtgtggc gtgatcaatt cccctaaacg cgcgccagta tgtttttctt
    45661 tcttgattaa acgatagcga atggcaggtt gcgtcattca tttccctact ttctatataa
    45721 aatatcttga caactcccac catcataatc gtttcaagtg ctttttgcaa gttccctcgg
    45781 cttccttcta ctttatttaa tattcttttt tttatccttt ttttatgaat aacaaaaaaa
    45841 agaaaaacat tatgacagaa atattttttg atacatggta tattggtgtt attaaagaaa
    45901 aggagggaaa aatcatgcaa tcttctgcag aattaaaacg ccaagcaaaa gagagtctaa
    45961 aaggacgttg gggacaagct gttttactaa atctaatccc tacattgatt ggtattgcta
    46021 ctattcttct tattatgatt cctattatct tgtttgccac caccatatat gatggatcag
    46081 cgagcgtctc agaagcgaca acttcaggag gcggtagttc aggaggcggt ggcggaatta
    46141 tttccagcat cattagcgct ttgttcatga gcggtatttc ttggacttat cttgatattt
    46201 tacgtggaca aaaagatcag atcgaaccat tcaaagatgc atttcgtgga ttcaaaggtt
    46261 tgtttatcgg cggagtgatc agcttagctg tgctgatctc gatctttaca acattatggg
    46321 gtttgctatt agttattcct ggaatcatta aaagctattc ttattctcaa agctatttta
    46381 tttactacga tatcgtccaa gaaacaggac aaacacctaa agtactggat acgatcactg
    46441 caagtcgaaa attgatggat ggttataaag gacaactttt ctggttagat ctttcattta
    46501 tcggctggca tatcttagca ttagcaactg ctggtattgg ttatctatgg ctcagcccat
    46561 acatttctgc aacaaaagca gcattttatg aacaattgcc aaaagatatt taaaagagag
    46621 tgtgacttgt gaccgagggg gtggcaggag aacaccgttt atcgagaggc agtaaaagaa
    46681 acattcagcc cattagcaaa aaggaggtgt gacaacattc atgtcacacc tcctttttgt
    46741 atagttccag taaaaagaaa atcaacttag aaacagttaa tcattcctct tcttctgctt
    46801 ccggtaatcg atggaacttg atcagttcat tcaccgcgat tggtacgata gataaaatcc
    46861 ctaccaagaa ccagtgattc aaactgatgt gtactaggcc gaatagttct tgcgtaaatg
    46921 gcagcaaagc aacagccgtt gtaatagcta atgccagtaa cgccatttct accaaagctt
    46981 tattggtcgc taaattgact ttgaagatcg attggctgct gcgtacattg aacacatgca
    47041 gaatcgatga ataagctaag atcaagaatg caacagtttg accgacatgc tgacttgcgt
    47101 ctacccatgg gctgatagta tcgacataac tacctaaata ataaccaaac aaggttacga
    47161 tggtgaagac ccctgcattt atggcaattt tttgcaataa acctcgagca aagatccctt
    47221 catttttcgg aatgggtggc tgatccatga tgccgtgttc cgctggctct cttcctaaag
    47281 caaatcctgg aagtccatca gcaactacat tgacaaacag tagttgcaca gctgtaaatg
    47341 gtgctcccca tcctaacagc atcgcaatca aaacaataaa gatctccgaa atattacagc
    47401 ttagcaaaaa gttgatagct ttacgaatat tctgataaac cgaacgtcct tgactgacag
    47461 cgtctacgat cgtcgcaaag ttgtcatccg tcaaaatcat atctgcggca ctttgcgcca
    47521 catctgttcc tgtgatcccc atggcacacc caacgttact tgctttcaac gctggtgcat
    47581 cattcacacc atccccagtc atcgctacga ctgcacccgt acgttgccaa gatttgacga
    47641 tccggatctt gtcttctggc gttacccgag catagacaga gagagctttg acacgcgcat
    47701 ctaattcttc gtctgataat tgatgcagtt ctgatcctga aagtgcttct gacggatcat
    47761 tcaagatacc taattctttc gctatggcac tggcagtcac cacatgatct ccagtgatca
    47821 tgactgtttt gatgccagcc tttttcgctc gcttgattgc tcctttactt tctggacgtg
    47881 gtggatcgat cattccgatc agaccaatca aacgtaagtc tttttccagt gcttcagaag
    47941 aaatatcttg cggttcttcg tcataagtag cataaccaac agcaatcact ctcagtgccc
    48001 gtttaccaaa gcgatcatta acgatcgccg cttgttctac atcgcctgaa ctaaagcgcg
    48061 gagcaagaac atcgaatgct cctttggtga ttgagatgta tttttcgccc atttggtgaa
    48121 ctgttgtcat catttttcgt tcagaatcaa aaggaagctc gtttactcgt ggatattttt
    48181 cttctaattc tgcctttgta tgataattct cttcaacagc ccttacgatg gctacttctg
    48241 ttggattacc tttgataaca aggtcgtcat cttcttgctc gaccgtcaca tctgtacata
    48301 gtgcagccat tttcaatatt tccatcgcgc tgttcgtcat tgaatcttcg atattcgtta
    48361 cttcatcttc tttgctccat acccggcgga cgcgcatttc attttgtgtc agcgttcctg
    48421 ttttgtctga gcagataaca ttagcggttc ctagcgtttc aactgctggc aactgtcgga
    48481 tgatcgcatg tttcctcgcc atcttctgca caccgtatgc aagtgtcaac gtaacaataa
    48541 cagtcaaggt ttccggtaca gctgcaacag ccaaggaaat cgaagtcatg aacatctcaa
    48601 ggagtggttc tccttgcagt tcaccaatca caaatactaa agccgcagcc gctaaagcga
    48661 tcatactgat tcgtttgcct agctgattca atcggacctg taaaggtgtt ttttgcatag
    48721 tattttcgtt caaaagatcc gcgatcttac ccatttctgt agccatgcca atggcagtga
    48781 cgatcgcttt cccacgacct gcagcaaccg tacatccttt aaataccata ttaagttggt
    48841 cacctaatga ctcatcttct tcagcaataa atgctgcatc tttttcaact gcttcacttt
    48901 ctccagtcaa cgccgattct tctacacgta attgtgatgc ttcgatcaaa cgggcatcgg
    48961 cagaaatagc atcaccggac tctaaaacaa gaatatcacc cactaccagt tgctctgact
    49021 caacagtggt cactttgccg tctcgtatga cagttgccgt ttgtttattc atatcttgca
    49081 aggattccag tgctttttct gcgtttcctt cttggacaat cgccaatacc gagttgatca
    49141 caacgatagc gatgatcagc agtccttcaa ataaatcccc atgttctgta gcaaatgcgg
    49201 tataaaagga aatcaccgct gctacaagaa ggatgatcgt tgtaaaatcg gacaagcttc
    49261 gaaagaattt tttgatcatc gattctttcg gcgcctcatc gaatttattc aatccctgct
    49321 tttttcgccg ttcatccact tcttgtgaag acaacccagt atctggatct gcttctgtta
    49381 ccttctttac ttcttctatt gtctgcttgt attcttccat cctattcctc ctttaaaatc
    49441 ttcgtacaaa aaaaggtact tattttacta taacatcctt tttttattta cttcaaataa
    49501 agggcttatc gattatttgt taagaaagcg taaaatttaa agtgcttata tttgcaaacg
    49561 ctaccaaagg atgctattat atagatgtaa ggaaggagga taccatcaaa tagacagaag
    49621 aatttctcga tagaacagtg taagcaaaca aagttgttag aagtacaggt ttcacccaga
    49681 catgcgtgta gagtacaaac ctataaatag tgaatgcgta tggatgtttg gatcacgaag
    49741 tatccgaaac aagaagtaat gcgatacgta acaattgaat aacgtaaacg tctgaaaatt
    49801 gaataggaac aagagatgtt tcaaatgttg aagatgtaac gaaaacaaaa tttttgttta
    49861 acatgaagaa attcaacacc gtgctttgca tgtatgatgg aaagttacaa tcatttctgt
    49921 ttatttgaat ccttccgagg tacaagaaag ccgagcttat cgagagctcg gctttttctt
    49981 tgtcataaaa tctttgtaaa gcttttttgt tattcaaaac tagcggcttt tcataacctt
    50041 atttcacgaa catcgcatct ccaaaactaa agaaacgata gcgctcttca atcgcatgct
    50101 ggtaagcaga taaagtcaat tctcttcctg caaaggcact aactaacatg actaaagtcg
    50161 attttggcaa atggaaatta gttgagaaag cttctaccac tttgaattca tagccaggcg
    50221 taatgaaaat atcagtccat ccgctgtctg ctttgatttc tccattaaat ttcgttccaa
    50281 tcgtttctaa tgttcgaatt gaagttgttc cgacagccac gattttcccg ccatttttac
    50341 gtacttggtt caatcgctca gccgcttctt ccgtcaaacg gtagaattca ctatgcatat
    50401 ggtgttcttc aattttatct acgctgacag gtcggaatgt acctaaccct acatgtagtg
    50461 tcagatagac taattcaaca ccctttgctt cgatctcatc aagtaattct ttcgtaaaat
    50521 gcaatccagc tgttggcgct gcagcagaac cattttcttt cgcatagaca gtttgatagc
    50581 gatctgggtc ttccagacgt tctttgatat atggaggcaa tggcatttct cccaatgatt
    50641 caagaatctc tagaaaaatt ccttcatact caaagtcaat gatccgtccg ccgtgttcca
    50701 attcttcttt cactactgct tgcaatctgc catcaccaaa agagattctt gtcccaactt
    50761 tggcacgttt cgcaggtttg atcaatgtct cccacgtatc tccttgtgta ttcgttagaa
    50821 gaaggacttc taaatgcgca ccagtatctg gtttttctcc atataaacga gcaggcagaa
    50881 ctcgtgtatt attcataact agcgcatcac caggctgtaa ctcttcaatg atttcatgga
    50941 aatgtttatc ttccatctct ccagttttcc gatccaaaac aagtaaacga gaatgatcgc
    51001 ggtcttttaa tggtgtttgt gcgattaatt cttccggcaa gtcaaaatca aaatcttctg
    51061 ttgttaacat atcgtcactc actttctatc atgtgcttca tcattctatc atgtttttcg
    51121 ttctgttggc aaaatcagca ctttttcact tctatttttt gttttcctaa ataaaaaaac
    51181 taccctattt gtattttttc attgttttag gcatactagt aatagatagt tcttcattga
    51241 aaatagaaaa agaggtggca ccatgattga atttcaaaat gtgtcaaaaa tttataaagg
    51301 tggaaaagtc gctgtagagg acgtcaatct ttcatttgaa aagggtgagt tcatttgttt
    51361 tatcggtacg agcggaagcg gaaaaacgac gacgatgcgg atgatcaacc gaatgactga
    51421 tccaacacaa gggaaaattt tgattgatgg aaaatccatc caagaaattg atccagtgga
    51481 attgcgacgt cagatcggct atgttatcca aagtattgga ttgatgcctc acatgacgat
    51541 ccgtgaaaat atcactcttg tccaaaaatt gttgaaagtc agtcaagaag aacggaataa
    51601 aacagctgaa aaaatgattg atcttgtgga attgccccgt gagatgttag atcgctaccc
    51661 acatgaatta tctggtggtc agcagcagcg tattggagtc gttcgtgccc ttgcagcgaa
    51721 tcaagatatc atcttgatgg acgagccatt cggtgcgctt gatccaatca ctcgtgattc
    51781 cttgcaagac cttgtcaaag atttacaaga gcgtttggga aaaacgatcg tatttgtaac
    51841 ccatgacatg gatgaagcga ttaaactagc aagccgtatc gccatcatga gtgaaggccg
    51901 tgtgatccaa tttgatacac cagaaaatat tttacgtcat cctgcaaacg atttcgtaga
    51961 agagttgatt ggagaagatc gcctactaca agccaagcca aatgcgacaa gagtcggcga
    52021 agttatgcta aatacagcaa tcacgattac accggaaaaa tcattacaag aagcaatcaa
    52081 actgatgagg gaaaaacgtg tagatacgtt gttagttacc gataattcga atgtattaaa
    52141 aggatttatc gatgtagaaa cgttgaataa aaaacgagga aaagtatcca gtgtcggcga
    52201 cattttgaat aaacatgttt tttacgtaaa agaatccgcc tatcttcgtg ataccttgca
    52261 acgcatttta aaacgcgggc tgaaatacgt gccggtcgtt gatgaacaga acaaagtcgt
    52321 aggtatcctg actcgtgcat cattagtgga tatcgtctac gatgtgattt ggggagaaga
    52381 tcccgcaact actgatgttg cagagtcagc tactcctgaa gaatctaagg aggtctgacc
    52441 tatgcagcag ttttttcatc agcacggttc agaaattctc tcaaaaggtt ttgaacatct
    52501 ttatatttca ggacttgctt tgatcattgg tatcatcatt gctgtaccgc ttggtgtctt
    52561 actgacacgt tttccaaaag ctgccacgat tgtgattggc ttaaccagtg ccttgcagac
    52621 aatcccttct cttgctttat tggcattgat gatcccgcta tttggcgtag gtaaattacc
    52681 tgcgattatc gcactcttta tttattcatt attgccgatt ttacgtaata cgtacattgg
    52741 gatgaaaaat gtcgattgga attatcggga tgtggcaaaa ggcatgggga tgactgagtt
    52801 ccaatccatt ttttccgttg aattgcccat tgctatgcca acgatcatgg ctggtattcg
    52861 tttagctgcc gtttacgtga ttgcatgggc tacacttgcc tcttatatcg gcgctggtgg
    52921 tttaggcgat ttgattttta gtggactgaa taattaccaa ccagatctca ttttggcagg
    52981 tacgatccct gtaacaattt tagcactatt ggctgattgg cttctaggtc tcttggaaca
    53041 ccggctgaca ccgatcgcac ttagagagga gaatgaagaa tgaaacgtat actaaaaatg
    53101 accatggttc ttttctctct tcttatactt gcgagttgtt ccttccctgg tcttgccagc
    53161 aatacagatg aagacaccat ttcgatcaca ggtggaatca cttcagaagc acagatctta
    53221 gcaagtctag ttgccggaat ggttgaacat tatacagata aaaataccgc aatcatcaac
    53281 aatctggcta ctacaactat caaccatcaa gcgatgatga acggagatgc ttcaatttca
    53341 gcggctcgct acacaggaac agatttaacg actacattga accttcctcc tgaaaaagat
    53401 ccgaaaaaag cgtttgctac cgtgaaagat gaatttgaga aaagatacgg tcaaacgtgg
    53461 ttcccgtcct atagttttga aaatacgtat gtctttcttg ttcgaaaaga caccgcacaa
    53521 aaataccatc tatctaaagt cagcgatctg aaaaatgtag cggatgaatt agtagctggt
    53581 gtagatactt cttggatcaa tcgtaaagga gacggttatg acggcttcca agaaacttat
    53641 ggcttctcct tcaattcgat attacctatg cagattggac ttgtttatga cgcagtcgaa
    53701 gcaggaaaaa tggatattgt tcttggctac tctacggatg gacggatcgc cagttatgat
    53761 ctagtcatgt tggaagatga tcgtcaattc ttcccaccat atgatgctgc tccagttgta
    53821 gacaacaaga tattagctga tacgcctcat ttaaaagaag ccttaaataa gttaggtcat
    53881 accatcagta cggaaaaaat gcaggaattg aactacgaag cagataataa tctagtggaa
    53941 ccatcagtag tagcacaacg tttcttacaa gaaaaccact attttgaagg gaagtgaagc
    54001 aattggataa aatgaatgtc tttcaacaat ttttctatta tttccaggaa aatggctcct
    54061 atatctttgc acaatttatc cgtcacttcc tgatttctat ctatggtgtc ctctttgcag
    54121 cagttgtcgg gattccggtt gggatcatga tttctaggag acgtaaatta gcaaactggg
    54181 tgattcgttt ggcgaatatc atccagacga tcccttctct agccatgatc tccattttaa
    54241 tcattggatt aggcctggga gtcaatgtcg tgattgttac tgtatttttg tattccctct
    54301 taccgatcat caagaatacg tataccggga tgatccaagt agacaaaaac atccttgatg
    54361 tcggcaaagg catggggatg actgctagac aacgactatt catggtagag ttaccactct
    54421 ctgtttctgt tatcatggca ggcataagaa acgcgttagt tgttgccatc gggataaccg
    54481 cgatcggtgc attcgtcggt gctggtggtc ttggagatat cattatccga ggaaccaatg
    54541 caacagaggg cacatcgatt attttagcag gtgcattgcc aactgcatta atggcgatca
    54601 tcacggactg gcttttaggt attttggaaa gacggctaga tccagccagc cgtacatcac
    54661 gctgatgtta ttgtaaaaaa caaaagcttc tgcgaccatt tgttggtcgc aaaggctttt
    54721 ttagttctgt catttttaag gcaataatcc actctgattc ttccctcctt atgggcaaag
    54781 agttgctttt cttagctatt tatccattcc gtatactcca acttcatact gccaacgggt
    54841 tcatatctgc atccaccgac taagtttttt tcttatccgc taaggcattt tgccaatcca
    54901 ttttcccatc actcgtcatg gaattcatac cacatgatct ctaaatactc gtctgcttgt
    54961 tctttgaatt gggcatcaat tcttacatcc tttggcgtgg ctttcattta agccttttcc
    55021 atcagtcctc gattgttcga tgccttcagc tgcatcatca aaactggtga ctgccgtatc
    55081 ggtaacttcg atcatctttt atacaatcgg gaacttgcca tcctcatcct tggctaaaaa
    55141 catatctagc cattccagct tctcgccatg ggaggtttgc ggttcctaaa accggtacac
    55201 gattcttatg gactgcatcc gtcgcaacta gacttggcga tacgatgatc cttcaccaga
    55261 agagcacccc cgtaaaccaa ttgatcgata tattgccaat aagaaaagac attggcgtaa
    55321 aagatatcga tccctcgtga tgcattgccg ttgatactgc tattcatggc cgaaagcgcc
    55381 acgactttaa cttcttccgt ttgagtgaca ttcatgttcg gcaatgtcgc tttggatacc
    55441 cgctctgcta aaggaacgac actggtattg tatttcgtat ctgtatcttt tccaaaagac
    55501 catgccaacg aatcctctgg gaaccaataa gacgactccg gttgattgtc cattgctttt
    55561 ttcaacatac ttttggctgt ttctcgataa acgacatttc caattgtctt cgcctcactt
    55621 tggcgataat cgatggtcgt tgccactgcc aacactaaca acgatgctcc aattttactg
    55681 attttccttg tcacacttct tcctccttgt tttacataaa cccgtcaaca ttccattcct
    55741 aaactcaaac aatgaccctt caaaggtgga gatataggaa caaattaaag aaatcatgca
    55801 ataaatccaa gaaggttggt tttagaacaa aaaaagagtc tcctttttgg ggagactctt
    55861 tgttaactat ctaaaaaggt tggctcttct cacttcactc ataatgtgtc caggcagaat
    55921 aaatttctac aactttattt tcctcatcaa ctttgtaaac cactcgatgc tgtctattca
    55981 gcctgcgtga gtatctgcct tcatgtgtag gtctcagctt ttcaaaagac tgcgtgggca
    56041 tatatggatc ttccttcaat gtctgaatca cttcttcaaa ttgtttcttt aaattcgttt
    56101 gcttgatttt tcttaaatcc actttcgcag agtttttgat tgcgactgta tagttactca
    56161 tcaaagagct tcccaatcaa tatcatcgat attcgtaaag ctactgtcgt ctttttcgcg
    56221 gtctcgtact ttatctaaag tacctgtttg ctcaagaaaa agggtttctt gaatcgctct
    56281 ccagtcttcc agcccaatca atacagcatt gtttccacta cgatcactaa taatctcgat
    56341 ttcagtatga ttttcattta cctctttcaa taactgataa aaatttttac gagcagtgct
    56401 aggattcatc gttgtattag ccattgtctc tcctcctttt tctgatataa ttatagcgta
    56461 cttgattaag tacgtcaaaa aaatacaata aagcacttaa attcagcaaa cttttcctct
    56521 tttttacata gagaaaaaga ccccttttta gggaggatct ttgtccacgc tcacttctta
    56581 ctcctttacg actagcggta gccaaacgta aagtaattgg taacaacgta tcatctttag
    56641 caaaggtcga atccaaagcc tgtgagtcta tgcaacaaca ccaatacacg aaggttcctc
    56701 gttgcagtgt gacttgattg atatcttgaa taacctgagg atacgcacga aagatttgta
    56761 acggttattc aaaactgatt ttggccgtca agtcttctac tgcctcaaaa atgattgcct
    56821 ctttcgtctc ctgtcattca tgttcactat cgatttgata cttgcttaga ccgttgggtt
    56881 tgtgaatcac caattaaaaa tggcactgag ttatggtaac aaaaaatggt cacttgacga
    56941 ggctgagatt cctacttatg aagacacatg gcacttaaga gtcgaggtaa aggaagaagc
    57001 tgaaaatata ggaggctgta cattatttgg caaaggattc ggaaattatg atcaagacat
    57061 caaaatcaaa atgttttaca gctaaatagg aagaataata acatttcata cgctgttccc
    57121 atctttaatg gcttatagag ggcaccttta gctgagaata agacaaaaca gaccctctag
    57181 cttcaattaa gagctagagg gtctgttttt gattttaaaa cttggtttat ttcttcaaat
    57241 ggtagctata ggtgctgaca acgatatttt cacgccaaga caatcgcaat cttaaccgca
    57301 aacttgtttg gttatggaga atattctgcc agcgtctgtt tggtacaaat tgaggaatca
    57361 acacagtagt tgtatagttt tgtttcgctg catttttact tacgatatct acataacgga
    57421 taattggatt ttcaatcgag cgataagaag agtggacgat tgaaaaacgg acatctggga
    57481 aatgcttttt aaattctgct tcgatttctt tttctttttc gacatcttcg tctagcgaaa
    57541 catgcatcgc aacgacataa tcaccgatcg aacgtgcgta attcaacgca ccgatattta
    57601 cccgtgtcac attcccgact aatacaagaa cggtattccc gtcataatca tgtagttttg
    57661 catcattttc taaacgaagc tgttctgcta cttttttata atgatcatgg atcttataaa
    57721 aaatgaacat aacgattggc ataatgatga agaaaggcca aatgtcccct aaccgataga
    57781 caaacaaaat cgcaatgatt gcatatgaga tgaacgcacc cgtaatattg gcaatcgatt
    57841 tgcttaacca tctttttcct tcttttttcc atttgacgac catccctgtt tgagataaag
    57901 caaatggaat gaacacgccg atagagtata acggaatcaa gcgttctgtt gagccatgga
    57961 aaataaacag caatacgatc gaacctagcg ctagtgtgat aatcccattg gaatagccta
    58021 gtcgatcccc acgatcttga tacatgtgag gcatgaattt atcttttgcc agattgtatg
    58081 caagaactgg aaaagcagaa aaaccggtgt tagcagcaac tgctaaaatc agcgctgtag
    58141 caaattgaag gacgtaatac aaaataccat gtccaaagac agctttacca atttgagaca
    58201 aaacagtgac ttctttttca ggaacgatcc cataccaata gttgataaat gtgatcccca
    58261 cgaagaagaa acctaagatc aatgccatca tcgtcaatgt agcagcggca ttttttgctc
    58321 ttggcttctt aaagaatggt acagcattac taatcgcctc caccccagtt aaagaggaag
    58381 aaccagaaga aaatgctcgt aaaatcaaag cgatagaaac acccggcaca gccgctcctg
    58441 gaagtgcagt agcttgcaat ggcacagcac ctgtcacgat attatataaa ccaacaacga
    58501 tcagtaacga gatcacaagg ataaatgtgt acaccggaaa taataggaac gatgcagatt
    58561 ccctcagccc acgaagattc agcatcatca gtagcaaaac gattgtcaca gaaatagcga
    58621 cttgatgacc gtagagtgct ggcaccgctg atgtgatcgc ttcagcacct gctgagacag
    58681 atactgctac tgtcaacatg tagtcgatca ataaagagcc accggaaatc aagccagcat
    58741 tctttccaag attctcactg ctgacgacat atgctccccc gccatgagga taagcatgga
    58801 tgatttgacg atatgatagc gttaatgaaa tcaataaaat aataacaaaa gcggcaatgg
    58861 gtagcgaata ccagatggct gcagcagaca aagcgactaa aacgacaacg atttgttccg
    58921 taccatatgc aatagatgat aacgcatcag acgacaataa tgccaaagca gcaaatcggg
    58981 ttaatttgtg ctcatcattt tcagcagatt ttaaaggctt acctactaac aaccttttta
    59041 agtaatccaa tttctaaact cctttacaca caattttact tcaagtttta tagtcgaaaa
    59101 tttatagtaa acaagatgtt atagtacgct cactgacctt aaaagtcaat aaactttaag
    59161 aaaataattg gaaaaaaaca gaaaaatatc aagcgctttt tcaaacgctt taatacagca
    59221 aaaatctaac atttttttat gggctttttc tttgaattct ataataaaag gatagaactt
    59281 gacttagaaa atcaagttct atcctttttg atatcttaac ttttggaaga gagatgtaag
    59341 gctgctttct acccatcatt cttactactc gaagcatagc gcagttgtca caacctcttg
    59401 ataaacggtg tttaaaagct tgcatctgcg gtaataagcc caaccaaatt aaaaacataa
    59461 gaagctgttt ttatttggtc gttcttatta cttgatgcag agcagctttt ttacaacctc
    59521 ttggtacacg gtgttcaatc agctgaccct cggtattagc tcaaaaaaac ggcaaaatat
    59581 gaggaactat ttttccattt ttttctctaa tactcaggtc aaaacactga tttcacaacc
    59641 tcttttttta atgaaaaata tggctctcat gcgccgcttc tttgaatttc gtcatttttt
    59701 cttcgatgaa cggatagatt gccgttccta cgattccaaa gacaacaaac aaaccgatca
    59761 tgatccagat tgctttcgta gcatttggcc aataaattcc tcctgcagat tcccgtaaca
    59821 agtttaccgc atgagtaaat ggcaagaacg gattgatcat ttggaagaat ttccctgaga
    59881 cttggatcgg atagttcccg cctccgccag agatcgacag tactaagatg ataatagcga
    59941 tccctttacc gacattatcg aataatgcag ccaagacata gacgatcatc atgaacgcta
    60001 aggcgatcaa gacagcaaat gacacgctgt agaccggatt ttggacatct actcctagta
    60061 agaaaatatt ccctaatgta acgatcaagc tttgcgcaat cgccattgtc aaaaatgtca
    60121 gcatacgagc aacgaattgt tctcgtttcg taaaattgtt tcggtctttc tttcctaaat
    60181 agtattctgt cgtagcaaca cttgaaagaa gaacagcgcc gacccacaga catagtgcag
    60241 tataaaacgg ggtgctagct gaaccgttat ttgcgattgg ataaagcgca ttggtttgta
    60301 gctcaacagg ttgtgtaaag aagtcacttt cttttgttgc gtctagtttc aataatttga
    60361 tgacttcgcc tagatcgact tcttgttcgc cttttcggat cgcgtctgct gctttttgga
    60421 taccagcttt gatatttggc cagtcattgt tgatcaagtc cgcagcagcg ttcacagctg
    60481 tttcgacttc tggcattttt tcatttactg ttgtcaatgt accggtgatt tcatttttta
    60541 gtgctggcca atcattttgg ataaaatcag cggccaatcc taatttttgc tgaatgatcg
    60601 gtaattcatt gttgtatagg tctgcccctt cattgatgcc attgacgatg gtatccatat
    60661 ttccattcag cattgtattg gcatcatgga tttcttgtcc aattgcaggc atttcttttt
    60721 gatatttttc taaaattgcc actgcattcg ttacagttgc tttcgttgag ctgagaagcg
    60781 aatcaaagtc gatctggtcc gctttgctca gtgcgccttg tgcagtccca atcgtatcga
    60841 tgacttggtc tagtagagaa gtgacagtgg aactgatttg acctgtattg ataccagcaa
    60901 tgatgccatt gatattatta acaacggcat caatttgatc tagcaattca tcaatagcct
    60961 tcgtgtcttc attttgaaca gctgtgttca aagcagatag gcggttttgc agattgctta
    61021 gcaagggttt taattgattt agctgatcaa taattggctg taatttcgta tttccagcac
    61081 ttgtttgcaa tttagtcagg aactcgacca gtttatcgat tgcattttgt tgttgagata
    61141 ttccgtcaat aaagccgttc aatacattgc tgatgttttt cttttcatca tcagtcagct
    61201 taccatcttc aatagctgtt cttacagtat ctattaacga acctgtcgtt gttgccactt
    61261 gtccaattga cttcaatgta acatctacac tgtttgtgat gcttggtatc gcatctttca
    61321 gctgagaggc acctgatttt gtttggtttg ctagatcact tgcctgattt tctaaatttt
    61381 tcacatctgg caagatatct tgtacttgtt ggatgatcgt caagccttgt tttgcttcat
    61441 cgattccttg gttcattgtc tcttggatcg aagcaaaatc gccgtcgatc tctgctagtt
    61501 gcttaccggc attttggatc tctgggattt tttcttgcaa atcaaggatg atttttgctt
    61561 gatctttcaa ctcaggcatt ttgtcattca atgcaacgac tttttcaccc atctcatcta
    61621 ctttagggat gtaatccacg aattcatttg ctttattcag tttttccttg atttcaggta
    61681 gttgactttg taattcgagg acttgttttg tgtagccgtc gatcgtgtcc aaattttcat
    61741 ctgttgataa aatcatatct ttaactttat tgatacttac taggttcgta tcgatgtcgt
    61801 aacctatctc attgaatact ttcaataaag tcgaactggc tgttttgata aattcatttg
    61861 tgatttgcga ttggatcgaa cttgctcctt tatcggtgat tttcggtgca atcgcattga
    61921 ttttttcatt taccgtatat tcgatttttg gtttcttgat atcaccacta gtaaaactca
    61981 gcagatcttc tgagaaatcc tttggcaagt aaatacctgc gtaatacttt ccggaacgga
    62041 ctccgtcttc taattgttct tttgaatcaa cgaattgcca tcctaattgt ttatttttat
    62101 gaagcgattc gatgacttgc tccccaatgg ccacttcttt accttgaaac tctgctggct
    62161 tgtctgcact gtaaacagca attggcagct ctccagtatt tccatatgga tcccataatg
    62221 ctttgatgtt aaaccacgca tatagcgaag ggatgatcat gatcgcgatg atcagaagtg
    62281 ttgcaatcgg gtttttgaaa atccgtttcc agtcaagcaa atataatcga agcgtatttt
    62341 tgattgactg catagttctc ccccttcttc ttttttcagc tgaattactt taccatgtat
    62401 ttcatacttt tttcaatcgg cttttttcaa aaaagtcgaa tttttggaca tatcacggca
    62461 attgtctcat tgtaatctct tacatattga gaaataatta aggattacag cctttacttt
    62521 atcatgaaat aaaagggaaa gataatttta tgccaaagtt tttatctatt atacgtatac
    62581 gcaaaaaagc taaaacattt tttgtctttg cccctacaaa aatgtttcag cttttttcgt
    62641 tttttattcc tgtatttctt atttttcgaa tgacaggatg attttcccag caggaggatg
    62701 tccttcaact agctgatgtg cttggatgac atgcttaagt gaagcagaca gattcattga
    62761 aataaaaaca tgaaaggctc ctttttggta ggctttgatt atgccagcaa aggcttctgc
    62821 atctagatat tcctttcttg gaacaaacga ttcataaaag ccttcctttt tttgccgcaa
    62881 atctagatct gggaggtcat ttaatgcaac atacctgccg cctggtttca tgatctggat
    62941 gccggtttct cctttgatac ttcctttcgt cgcatcgatt acaaggtctg cctgatcagc
    63001 aaattgcaga cctggatttg ttttatcata agcagcaaat gctgaagctc ctaatttttt
    63061 taccttttcc tcatttttac tggatgcact ggttaagatt cggattccct tttcatggag
    63121 aagctggatc agacttgacc cgaccgcacc tgatgctcct agtatcatta cagtatctgt
    63181 cggctggatt tccgtcaaat ggttgattag attataagcc gtaattcctg gcgtgaccat
    63241 tccggctgct tcttcccaac tcattttatc tggaattttt gctacttttg ctgatggcaa
    63301 cacgacttct tctccgtaag tcccgccgac tgcatggaca gctactcgat cacctacatg
    63361 aacagttgtg acatctgaac caatctctgt cacaatacca gcgccatcat tacctggtac
    63421 ataagggaat ttcaatgttc gtatctcttt catttctcct aaacgcaagg caacatcata
    63481 aggattcaca ctgaatgctt ttatttctac acgaacatgt ccagaaccga cttctctggg
    63541 atgagcatcg atttcttcaa aaacatcttg tgcttctccg taaccgttaa ttgcaaatct
    63601 tcgcatggtc accatctccc tttttcttta agtttacgtt tatgccttta ttttgacaaa
    63661 aaaaatgtct tttcgttttg gcgcatgatt caaaacaaaa tagttgtctt ttttcgcgta
    63721 acgtgttaaa ttatttatct aaaagcgtaa agattacgat ttacgctaat aaaaaataat
    63781 tggaggaatt tgttgatggc taaaaaatca aagattgcca aagcaaaaaa acagcaagaa
    63841 acgattgaac gctatgctgc tttacgccag gaattaaaag cgcaaaaaga ttacgaaggc
    63901 ttgcgtaaac ttcctttaga tgctcgtcca gaacgattga aaaatcgtga tttaatcgat
    63961 ggacgccctc gcggatacat gcgtaaattc gggatgtccc gtataaaatt ccgtgaatta
    64021 gcacataaag gtttgattcc aggagtaaaa aaagccagct ggtaatccaa gtctggctgt
    64081 aatgtaaagg aggagggcgc atggaccact ccttggatca tcaaaaaaga tacgttcgct
    64141 tcttctagca gaaatgctgg tactagaagc agacgtatct ttttttacgc acagctggtc
    64201 cttttttata tgtaaaggct gcatggttac ttcgctatat ttatttagta acaggagtca
    64261 gtgcttcgat acccacacta attttcaatg ctcggtcaac ttctgccata atgggtcgat
    64321 ccaaatggca gaccttttct ttcaatcttg atttatcgat cgttctgatc tgctccaaca
    64381 ggataacgga gtctctttct attcctgttc cagtagcttt gatcccaatg tgagtcggaa
    64441 gcttaggttt agccattttt gccgttatgg ccgcaacaat gatcgtcggg ctaaaatggt
    64501 tacctagatt attttgaata accaaaactg ggcgtgttcc gccttgctcc gatcccacga
    64561 ctggagaaag atcagcaaaa taaatatctc ctctttttac cataggatcc tccttattct
    64621 tgatattctc ttggtatccg ctgtcctaat ccgcaagcca cttcatagtg gatcgtacct
    64681 aattgatctg ctatatcctg catagtgatt tctttgtctg catttttgcc gataagcgtc
    64741 acttttgttc cgacaggaaa ttcttgaggc aatcggatca tcagttgatc catacataca
    64801 cgcccaatcg tttcacagta gttcccttct accaatagag aaaaaccctg catctttcgt
    64861 ggccagccat ctgcataacc gatcggtatc gttccgatcc actctgcttc aggagtgata
    64921 tatgtttctc catagccgat tccttcacct gctgggagtt ttttgacttg aatcaattca
    64981 gaaaccagct caagtgctgg ctgcaatgga taaacttccg gtagtgcgtg cccagaggga
    65041 ttaagtccgt acattgctac tccataacga atcatattgc cgattgtttc atcatgccat
    65101 aaagccgttg cactattgct gacatgtaca tatcgaggaa gttcgggaag ttttttcagc
    65161 acttctataa agcgttcttt ttgcagattc caatatgtat catctgcctg atctgctgta
    65221 gaaaaatggg taaataaacc ttcccattct aatacacgac tttctttgat gaatgcagct
    65281 gcctctttta cttcttcagg tgtacaaaat ccgatacgac ccatcccagt gtctgctttt
    65341 agatgaatcg aaagaggcgt ctctagctta cttttctcta aaacagcagc tgctgcttgc
    65401 aaccattccc gtgtacctgc agttactgat aaatcatgag caacagctac agtcagtgcg
    65461 tcgtaaggaa ccatattcaa gataaggatg ggctgagtaa atccagcttc tctcaactcg
    65521 actccttcat ctaaatttga aacacaaaag cccgaagctc cacctgcaac tgctgcctca
    65581 gctgtttcaa tagctccatg accgtatccg ttcgctttta ctactgcaaa caattctttt
    65641 ccttgcggaa gtcttgcaac ttcatttgct acattttcag ttatagcttt tttatgtatc
    65701 actgctttcg ttgggcggtg ccaagcgaca accatcgttt ttccttcttt ctagtcttcc
    65761 aaaataattt gtgcaaaagc tgtttcattt gtatgagtaa tactgaccca tacattgcct
    65821 tcatgaggtg aacgcgtcac aacaggctga ccattttcat tttttaaaat ctcgacatct
    65881 tgaaaggtaa cttttccgat cccagttccc caagctttag agaaagcctc tttgcaagcg
    65941 taacgcccgc ctaaaaattc tacttggcga tgaaaaggca aggactggaa tagtttcatt
    66001 tcatcagagg tcaagattcg tgcaatgaat tttggttttt cttcgatcag ccgagtgatc
    66061 ctaggaagtt cgactgcatc gatcccaatt ccttttatca tatgcttttc tcttttcaat
    66121 aaatttagaa cactctcatt ctaccaaatt ataaccattt tgccacaaat aagtccacaa
    66181 ataacaaaaa tgaaagtttc tcttaaaaaa gagaaacttt cattttatgt ttaatcgtta
    66241 tttgaacgga ttacaaatcc gcgtttttta tcattgtgac gtttaccgcc atctttttgg
    66301 ctgttattat ttttgctata accgcctttt gatttgtttc cacgataatt accgccgtca
    66361 cgacgattac ggttgtttcc gccaccacgt ttgccatttt tattgtatcc ttttttctgc
    66421 attggcaatg ggcgttctgg tgtgatcttc actggcactg catcagctgg atcttttgct
    66481 gtcgttttta gcaaaagtgc aactaaatct tgtgcagaat attcttctaa taatttatct
    66541 gctgtttgca ggtatttatc tagaccattt tctgcaagtt ttgtttcgat ttgttcaact
    66601 gcagcaccta attgaccttt gaatgcttct ttttcggttg gtggacgtaa agtcgtcatc
    66661 cgttttttcg tcaagttttc aatcacatgc aagtagtcca tttcatttgg tgtaacgaaa
    66721 gtcacagaca taccgccttt tccggcacgg cctgtacgtc cgatacggtg aacgtagctt
    66781 tctggatctt gagggatatc atagttgtac acatgagtga cacctgaaat atctaatcca
    66841 cgtgcagcaa cgtctgtcgc tacaaggata tctaaatgac cgctcttaaa tgaacgcaat
    66901 acgctcattc gtttttgttg agacaaatcg ccatggattc cttctgcacg gtaaccgcgt
    66961 gcttctaatc cgcgtgccaa ttcgtctaca cggcgttttg tacgtccgaa gacgattgtt
    67021 aattcaggtg tttgaacatc aaacaagcga gtcatgatat cgaatttttc gtattctttt
    67081 gcgcggacat agtattgatc gatcaagtca gctgtcattt ctttcgcttt gatcttcaca
    67141 tgatgtggat ttttcatgaa tttaacgcca atattcttga tagctggcgg catcgttgct
    67201 gagaaaagta atgtttgtct ttgatcagga acttgtgaaa tgattttttc gatatcttcc
    67261 aagaagccca tattcaacat ttcatctgct tcatccaaaa ctaatgtttg aacggttcca
    67321 agtttcaatg tgtggcggtt aatgtggtca agcatacgtc ctggtgtacc cacaacgata
    67381 tgtggacgat ctttcaagcc acggatctga cggccaatat ctgctccgcc gtaaacggct
    67441 tgtacacgta cttttttgtc gcgccctaaa cgatataatt cttcttgtgt ttggatagct
    67501 aattcacgtg ttggtgcgat aactaaacct tgtaattcgt gacgatctgg atcgattttt
    67561 tctaacattg gtaatccaaa tgcagctgtt ttccctgtac ctgtctgtgc ttgtccaata
    67621 acgtcttttc cagctaatgc tagtggaatc gtttctgctt ggataggcgt tgcttcttca
    67681 aaaccagcgc gttcgactga tttcaacaat tctggtgata attctagttc tttaaatttc
    67741 aaatgaatcc tcctatatgg ttgttaactg aggatgtagc ttttttgcta ccccgtcgat
    67801 ttgttcggtc aaagtgtatt gtttcttttt gacagtggcc acgcacgttt ttatccattc
    67861 ttagcccctg tggcaccacc gaaaaatcac tttgcgaagt ttaggcatta gaaaagccaa
    67921 acgggtcgcg ttcagcttta tcattattat gacatctggc ttcaacaagt tgtttctatg
    67981 gcaaaaccag tccaatttct cttcatcttt cggcaatagg ttattctaac atagtttttc
    68041 gttttctgca agtttacaga tttttatttc atttgctgaa gttgttctac cacttcaaat
    68101 aagcccatgc tgttgctgcc ttttagcata atactatctg ttgcttcaag attttgtttg
    68161 atagcatcaa tcaatttatt cttttctttt tcaaaataga acacaggtag ttgggggtat
    68221 ttttggttca attcttcttt tagtgcatgc atttgttccc cataaaggaa aatcatggcg
    68281 aatttctcag gatcgatatg ttcactcatt tgcgcatgca tatttaaaga ctctttccct
    68341 aattccagca tatctgctaa cacagcgatt ctgcgtcctt ttgttggcag atttttaaaa
    68401 gtatctaata ccagtcccat ggctgttgga tttgcattgt agacatcgct caaaatagct
    68461 gcaccatttc ctgccgttag ccattcggtt ctgttttgag tgatagaaac atgggcgagc
    68521 ccttggcgta tctcaggtat ggacagtcca aagaagcgcc cgatcgtata cgcaatcaaa
    68581 gcattcgtta cattataact tccaggcagc gggatcgtaa atgtttctcc ttctactaca
    68641 aaatcggttt gtgcttttcc ttttgctgtc acttccgcat gaacatcgcc atttccaata
    68701 ccgaatgttg tgactgtttg tactaatgtt gccgttaaag gtgtcagcaa cggttcatct
    68761 gcgggcacaa cgagtaatcc gtctgataca agtccgtcag tgatttccat tttggcttta
    68821 gcgattcctt ctcgcgaccc aagattttct atgtgtgctt caccgatcat tgtgattgct
    68881 gcagcttttg gttgagccat aagtgaaagt tccgtgattt cattggcgtg atccatgccc
    68941 atttccaaaa cgatcttttc tgtatcttgg ggcatgtgca aaatcgtgta aggaagacca
    69001 atattattgt tgtaatttcc ttgcgtctta tatgttttgt atttttcaga caagacagac
    69061 gcggttagat ctttcgtggt ggttttacca ttgcttcctg taattgcaac gacttctgcg
    69121 cctacttttt ccaagtaata ggttgccaat tgctgcatgg ctttcaaagg atctgctaca
    69181 ggaataactg gaaaattttt tggagcttgt tcaacaggca agctccaaaa tgaagcagat
    69241 gctccgtttg tgatcgcttg ctggataaat tcatgtccat ctcgcgtacc agcaagagga
    69301 acgaataaac ttccttcggt tatcttccgg ctgtcaaatt cgacattggt aatctttgtc
    69361 agtgcagagc attcgctgcc cacggcttgt gcaatttctt gtattgttag attcattgag
    69421 atctctccta aaaaaggttg tgaaaaaagc gtttggccta aacggcgggt tcgcatgact
    69481 ctcacttcca agtaaatttc tctattcgta tttagaagct agaatgaacg cggttacgca
    69541 atcactccag cctcttcttt atttctcttt attcattttt ttcaaaaaaa gattgacgct
    69601 gatggtatcg attcattcct aactggatca gttcttcaat caaatcaccg tatttcaagc
    69661 ccatattttc ccataagagt gggtacatac tgaactccgt aaatcctggc atagagttta
    69721 attcattcag gaataattca tttttatttg tcaaaaagaa atcgcaccgg ctcaatccgc
    69781 ttccacctaa catcgtgtaa gctaacttcg cgtactcttg cgctttttga taaacttctt
    69841 ctggcacttc ggctggaatc tgcatttcga ttttattatt gatatatttt gcttcataat
    69901 catagaatgc tacgtctttt acgacttcac caggcaaagt cgtccgaacg tcttcatttc
    69961 ctaatacagc aacttcgatt tcgcgcgctt caattccttg ttcaacgatt gctcgagaat
    70021 catactgata ggctgttgct aaagcatttt gcagctcttc tcggttttct gcttttgtaa
    70081 tgccgacact agaacccata ttcgccggtt tgacaaacat cggataaagc aaagaacctt
    70141 cacattgatc aaatactttt ttaggatttt ctttccattg attcttaagt actggtacat
    70201 aaggaacttg cggcacacca gcagcttgta aaatatactt ggtcatgatt ttatccatgg
    70261 cacatgcact ggtcaatacg cctgcgccga cataaggcat attcaatgtc tctaagaagc
    70321 cttggatcgt tccatcttcc ccgtttggcc catgtaaaac tggaaaaacg atggctcctt
    70381 cttctttgat ttcgcccgga ttgatcactt ttcctgtaaa gccttcctct gtctgtccac
    70441 ttgggtccca tgaaagatgc aagacatctt tgctggcagg tttttctgtt agtaatggac
    70501 ctttgaccca ttgtccttct tttgtaataa atacgagttg aacttggtaa taattataat
    70561 aaatggcatt taaaactgaa aatgcggaaa gaatggacac ttcatgctct gcgctgcgtc
    70621 cgccatatag taaagtaatc ttcaaaagat tgtcctcctg tattctaaat cgttcagtga
    70681 tttactctca tattttagca caaaacaaaa ataaacgaat aacttattta taaaaaaacg
    70741 agccagcttc taaaagaacg agcgtcattt attttttgtc agctagatta tttgtcccgt
    70801 tttttataaa aagttatgat aaagaaaagg agggatgatg atggcgcaaa caaatccacg
    70861 cagccgcttg gcagaaatca ttcgagtgtt catcaattac aatgtagtgc caaactttgt
    70921 acagcaaaaa aatcccgaac aggtaaaaaa agcctttgaa gaactaggcc ccaccttcat
    70981 caagatagga cagatgcttt ctgttcgaga agatttgctt tcttctgcct tcactcaaac
    71041 attcaaaaca ttacaagata gtgtacctag cgacacattt tcaacggtca aaaagacgat
    71101 cgaaacagaa ctttctcttt cattatcaga tatattcgat gatttttcaa aatcgccttt
    71161 tgcctctgct tctatggggc aggctcatcg tgccaagctg aagaacggtg acgcggtagt
    71221 cgttaagatc caacacccta acatcgccga agagatccga cttgatcttc aactatttga
    71281 acgagcgatc cctttgatca aatacattcc tgaaactagc gtagttgatc taaaaggggt
    71341 actccaagaa gtaaaacgtt ccttgatcaa tgaaatggac ttcttaaaag aaagtcaaaa
    71401 tggcgaacaa ttctatcaaa agaacaatgg ctggaaagag atccgcagcc ccaagattta
    71461 tgacgctttt tgttcgaaaa aagtgatcgt gatggaagaa atgtctggta aaaacttgaa
    71521 tcatttaatg aacgccgaaa acaaaactga aacttttatc actggaattc aaaacaagca
    71581 gctgaaacaa gaagttgcaa agcttttagt tgagaacttt atgaaacagg tgtttgatga
    71641 tggttttttc catgctgatc ctcatccagg gaatctgcta ttccatgtac taacaaaaga
    71701 agaacagaca caagctagcc gaaaaactga aacagtccat gaaaaagagt tcggttcctt
    71761 tgcttttcga gcctccactt ctgcagaaga ccctgttgcg ccatatacca tcaactacat
    71821 cgattttggt atgatggggc atctttctgc aggacttagg caaaaactca cacaagccgt
    71881 tttagcgctg tatacgaaag atgcttatcg aatcgaaaaa gcggtccttc gactttgtca
    71941 gcaagaaggc tcgtttgatg aaagtcggtt ccaccaagaa ctgacaagtt ttcttgaaca
    72001 gtattatgat tctcctattg atgaaatcaa cttgcaagaa gtattcacac atgtcgtgac
    72061 gatttgtcat caaaataatc tgcagttcga tcgtgatatc acgcttttgc tgaaagcatt
    72121 tgggacatta gaaggggtga tccgcgtcct cgatcctgaa gtttccctca tggaagtcgc
    72181 ttcgcctttt gctcaacatt attttctcac tcatctggat gtagaagata cattgaaaca
    72241 aagcggatta gacttattgg aagggatgaa ggctgcgcct aagatccctc aacagctcca
    72301 ccatctatta gaaatgtgga cttcgggaca aggaaaagtc aatttggaat tgaaaaagca
    72361 agacaaatta ttgagcagga tcgaaagtat gatcaatcgg cttgttttcg gtatgatttt
    72421 agctgcattg atcgtagggt cctccctgct tgtccaagca gcacctgtag aaaacgcaga
    72481 ggtcgtttcc ttattaggga tatttacgta tgctattgcc gcattcgtca ttattttttt
    72541 ggcaatcgat gcattgatcc aaatgtataa gaagaggaaa aagtagaaaa aggttgtgac
    72601 agaaagaatc agcttcaaga aataagaagg ggttttacat aaattgtttc tcaaattttt
    72661 gtgaaaattc ggcttatttt tcgaagaggc tacttctgaa acaccgagaa taaaaaatgt
    72721 gaaaaagctg ttctgcaccc agtaataaga acaatcaaaa agggaacatg gaacacattt
    72781 atcctgtttc cctgttccct ttttgatgta ttcgagcata aataaaatac cttcgttacc
    72841 tactatcttc tgtacttggt aaaccttagt caattgcctt ttttaacgct tgacgaaaag
    72901 acatatgatc tttctccgta aagggttttg gaccctttgt ccttatgcca gcttttctta
    72961 actttccact atgataacgt tgttctagaa gcgtgccgat attcgcttcg tcaaattctt
    73021 ttccagagtg atggaacact cgcacaccct tcggcaatac aagtgaagct aaaccataat
    73081 cttgcgtgat caaaaagtca ttcttcttaa ccataccgac gattttataa tccgcactat
    73141 cagctccgcg gtctacgtag acgatttgga catgattagc gtattctttg gtcgtataat
    73201 ggtccacact ggtcacgata accacgtcaa gaccatagga ttctcctaat tttatgacat
    73261 cttcttttac tggagaacca tctccatcaa tgaatagcct cattcttttt cgccttcttc
    73321 cataaatgat aaacagttac taatccgctc agtaaaagac caacattgat ccattttgcc
    73381 aagataagca gttgtacact ttctatcggc actgattcat ggttcagcgt gatcaataat
    73441 agatggattg gctctcccat ttcttttgct aaaacaaaca aaagcgtcat caataaaaac
    73501 tggtggatca cactcccttt attttgagta gtatacttga caacgatccg gcgatccatt
    73561 ggaatagctg tatcagtttg ataatattcc ttatttagcc agctgcctgt tactacgtgc
    73621 cagaaactaa gcagaaagac acctatcgca aaaccatttg gaatcaagag gaccaataaa
    73681 gaagaactca gtccaagcaa ttgcatagcc aatggagacc atgttaaacg tttcaaaatc
    73741 catggtccga ctatttttgc tgcaatcaag cctgctgcat aaggcaaata cataaaaatc
    73801 gaaagttttt ctaatccgta cagtatgcca acataaaaac tgctaaataa aaaaaggaaa
    73861 ttccctagca ttccatttag aaaagtaaac agatttagcc agcttggcaa cttccattcc
    73921 ttcttctggt gtcctaaaac aaacacgaac aagatgaaca gcagcaagaa accataaata
    73981 gcaagatcaa aaagcctttc atcgaataac aatcgggcat ttctcaaaag aaacaataag
    74041 ccaaagaata tcaaaaataa aacaagctct cgcgtagcta ccagatttct tttgatgtct
    74101 ttgaaatcca gttcgtaacg cggataatga ctaaccgtat ggtaggccat gacgtaaaaa
    74161 agtgtataaa tagtaaagct tgctgcaatc tgagtcggtg caggcaactc aatcgcttga
    74221 tacaggagaa atagccagat agctgcaaat aagtactttt tcccatccat attgataaaa
    74281 ccttgcgtct tttcgtgaaa attgaccgtg gtattagcag gtacaagcca tgctgcactc
    74341 agccctagca aaacacttga aaaataaaat aaaggaaaat aaaatacgcc taatattcca
    74401 agcaatgaac cagcaccacc aatcagcaga gcgatcatca gcaacgtgta actatctaga
    74461 ccaaatcgta gactgttgac taaaaatagt ccagtcatcc gaaaggcata aaacagcgta
    74521 aatggcaaaa tcgtcagcca attatgcgag tctgttagtc ccaaaaataa aagataagga
    74581 ataaaaataa ccgtgttact taagataaag ggcgttgttt gtgtaaaatt acgcccaaat
    74641 cgtttgagtt tttcgaacat gacaatggct ccttaaacag tcttataact aatctgctct
    74701 tcgattttag cattaaaggg gaaataaacc aattttacag aatatattct tgtaaaaaaa
    74761 aatacagaaa gaagcataag cttctttctg catttttacg gtacttgatt actcaagcag
    74821 acaagccctt ctccacaaaa gtcttgcccg acacccctgc atccggcagg tgataaagat
    74881 agaaatcaat tatcctgtgc ctccaaaatc aattaggagc tctgtaaacc agagtccttc
    74941 tcgacccgtc gcataaattt actttaacac agttcctatg tttatgcgag agcttttttc
    75001 attttataat cgaccatttg aaatccttga ccgatatctg aaataagttc ttctgtaatc
    75061 tcaaatcctt catgcttata aacctcgatt gcttgatgat tcccctgatt cactcgcaaa
    75121 aagatctctc tttcctttgt ttgcgcagca agttcttcat accagcgaaa aactgaagaa
    75181 gtcaatcctt gaccacgtac tttatttagc aaatataatt tacttaaata aatttttcca
    75241 tttatctctt cataagcagt gtaaccgaca gtcttttctt cgctttttaa taaaaagtag
    75301 gacacacctt ctgtcagttc tttttgaatc tgtttttttg actgataagt tttcagcatg
    75361 taatccactt gtttttgacc aatgatcggc acaaaaactt ccggccatac ttcttgtatg
    75421 actgccatca gctcatccag ttcattttct gttactttta ttttttccat acacccacgt
    75481 ccttttttac attataaatg accttgcgct tgatggaata aaaagagcat gatcatgcta
    75541 tacacaccca ccgaccacgg tttcaagaaa atcacaatct gcataaattc cctaaaagat
    75601 aatttcgtca aaccagctac tagacagatc aagtcgtctg gcgcaaaggg aagcagcaag
    75661 gtaacaacta gtactttttt aatatctttc gtttttcttg tcagcaattc atcaaatttc
    75721 tgatactttt ttggcgataa aataagctgg acaaaggcta cgccatagta acggacaagc
    75781 aaaaatccga ttgcttcccc aatcactagg ccaatacaac tatataaaag cccaaatcca
    75841 ttgccaaaca tcagcatgcc cgctactgag gagatccctc ccggcaaaat aggaatgatc
    75901 acttggatca cttgcaacag aacaaatatc atcactgcat acttgccaaa ttgctggata
    75961 aacgcttgca aagacgtgct cgatcgaaaa atcccatgat gataagcata aagaatcaac
    76021 acaaagaaaa aaaacagacc aataatgggc atgatctgaa gacatttttg taaaactgat
    76081 ccttttttag acatctatat tccccacatc ttttttcatt tattaaaact gcctcct
//
LOCUS       DADVXU010000003        75385 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.003, whole genome shotgun sequence.
ACCESSION   DADVXU010000003 DADVXU010000000
VERSION     DADVXU010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 75385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.003"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(154..279)
                     /locus_tag="KB953_000175"
     CDS             complement(154..279)
                     /locus_tag="KB953_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB6865181.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(413..1306)
                     /locus_tag="KB953_000176"
     CDS             complement(413..1306)
                     /locus_tag="KB953_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HBB6865182.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(1488..2414)
                     /locus_tag="KB953_000177"
     CDS             complement(1488..2414)
                     /locus_tag="KB953_000177"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HBB6865183.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(2499..3260)
                     /gene="pflA"
                     /locus_tag="KB953_000178"
     CDS             complement(2499..3260)
                     /gene="pflA"
                     /locus_tag="KB953_000178"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HBB6865184.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(3504..5735)
                     /gene="pflB"
                     /locus_tag="KB953_000179"
     CDS             complement(3504..5735)
                     /gene="pflB"
                     /locus_tag="KB953_000179"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBB6865185.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(6012..8462)
                     /gene="parC"
                     /locus_tag="KB953_000180"
     CDS             complement(6012..8462)
                     /gene="parC"
                     /locus_tag="KB953_000180"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HBB6865186.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(8474..10519)
                     /gene="parE"
                     /locus_tag="KB953_000181"
     CDS             complement(8474..10519)
                     /gene="parE"
                     /locus_tag="KB953_000181"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HBB6865187.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(10685..11119)
                     /locus_tag="KB953_000182"
     CDS             complement(10685..11119)
                     /locus_tag="KB953_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBB6865188.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            11246..11884
                     /gene="plsY"
                     /locus_tag="KB953_000183"
     CDS             11246..11884
                     /gene="plsY"
                     /locus_tag="KB953_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBB6865189.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(12016..12891)
                     /locus_tag="KB953_000184"
     CDS             complement(12016..12891)
                     /locus_tag="KB953_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBB6865190.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(12973..13764)
                     /gene="codY"
                     /locus_tag="KB953_000185"
     CDS             complement(12973..13764)
                     /gene="codY"
                     /locus_tag="KB953_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HBB6865191.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(13782..15182)
                     /gene="hslU"
                     /locus_tag="KB953_000186"
     CDS             complement(13782..15182)
                     /gene="hslU"
                     /locus_tag="KB953_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HBB6865192.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(15196..15744)
                     /gene="hslV"
                     /locus_tag="KB953_000187"
     CDS             complement(15196..15744)
                     /gene="hslV"
                     /locus_tag="KB953_000187"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HBB6865193.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(15781..16686)
                     /gene="xerC"
                     /locus_tag="KB953_000188"
     CDS             complement(15781..16686)
                     /gene="xerC"
                     /locus_tag="KB953_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HBB6865194.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(16884..18260)
                     /gene="trmFO"
                     /locus_tag="KB953_000189"
     CDS             complement(16884..18260)
                     /gene="trmFO"
                     /locus_tag="KB953_000189"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HBB6865195.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(18229..20307)
                     /gene="topA"
                     /locus_tag="KB953_000190"
     CDS             complement(18229..20307)
                     /gene="topA"
                     /locus_tag="KB953_000190"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBB6865196.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(20429..21286)
                     /gene="dprA"
                     /locus_tag="KB953_000191"
     CDS             complement(20429..21286)
                     /gene="dprA"
                     /locus_tag="KB953_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBB6865197.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(21342..22109)
                     /locus_tag="KB953_000192"
     CDS             complement(21342..22109)
                     /locus_tag="KB953_000192"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBB6865198.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(22102..22962)
                     /gene="ylqF"
                     /locus_tag="KB953_000193"
     CDS             complement(22102..22962)
                     /gene="ylqF"
                     /locus_tag="KB953_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HBB6865199.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            23222..23620
                     /locus_tag="KB953_000194"
     CDS             23222..23620
                     /locus_tag="KB953_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBB6865200.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            23899..24399
                     /locus_tag="KB953_000195"
     CDS             23899..24399
                     /locus_tag="KB953_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HBB6865201.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(24482..25033)
                     /gene="lepB"
                     /locus_tag="KB953_000196"
     CDS             complement(24482..25033)
                     /gene="lepB"
                     /locus_tag="KB953_000196"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBB6865202.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(25185..25412)
                     /locus_tag="KB953_000197"
     CDS             complement(25185..25412)
                     /locus_tag="KB953_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HBB6865203.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(25409..25927)
                     /gene="msrA"
                     /locus_tag="KB953_000198"
     CDS             complement(25409..25927)
                     /gene="msrA"
                     /locus_tag="KB953_000198"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBB6865204.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(25947..26660)
                     /locus_tag="KB953_000199"
     CDS             complement(25947..26660)
                     /locus_tag="KB953_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HBB6865205.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(26663..27559)
                     /locus_tag="KB953_000200"
     CDS             complement(26663..27559)
                     /locus_tag="KB953_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBB6865206.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(27717..28559)
                     /locus_tag="KB953_000201"
     CDS             complement(27717..28559)
                     /locus_tag="KB953_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBB6865207.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            28691..29344
                     /locus_tag="KB953_000202"
     CDS             28691..29344
                     /locus_tag="KB953_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBB6865208.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(29407..29934)
                     /locus_tag="KB953_000203"
     CDS             complement(29407..29934)
                     /locus_tag="KB953_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBB6865209.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(29954..30901)
                     /locus_tag="KB953_000204"
     CDS             complement(29954..30901)
                     /locus_tag="KB953_000204"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBB6865210.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(30913..32799)
                     /locus_tag="KB953_000205"
     CDS             complement(30913..32799)
                     /locus_tag="KB953_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBB6865211.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            complement(32959..35037)
                     /locus_tag="KB953_000206"
     CDS             complement(32959..35037)
                     /locus_tag="KB953_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HBB6865212.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(35050..35325)
                     /locus_tag="KB953_000207"
     CDS             complement(35050..35325)
                     /locus_tag="KB953_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865213.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(35687..36142)
                     /locus_tag="KB953_000208"
     CDS             complement(35687..36142)
                     /locus_tag="KB953_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865214.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(36296..37504)
                     /locus_tag="KB953_000209"
     CDS             complement(36296..37504)
                     /locus_tag="KB953_000209"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HBB6865215.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(37616..37948)
                     /locus_tag="KB953_000210"
     CDS             complement(37616..37948)
                     /locus_tag="KB953_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HBB6865216.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            38096..38971
                     /locus_tag="KB953_000211"
     CDS             38096..38971
                     /locus_tag="KB953_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBB6865217.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(39053..39634)
                     /locus_tag="KB953_000212"
     CDS             complement(39053..39634)
                     /locus_tag="KB953_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HBB6865218.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(39640..40899)
                     /locus_tag="KB953_000213"
     CDS             complement(39640..40899)
                     /locus_tag="KB953_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBB6865219.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(40901..41947)
                     /locus_tag="KB953_000214"
     CDS             complement(40901..41947)
                     /locus_tag="KB953_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865220.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(42182..42457)
                     /locus_tag="KB953_000215"
     CDS             complement(42182..42457)
                     /locus_tag="KB953_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HBB6865221.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(42700..44010)
                     /gene="der"
                     /locus_tag="KB953_000216"
     CDS             complement(42700..44010)
                     /gene="der"
                     /locus_tag="KB953_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBB6865222.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(44192..45421)
                     /gene="rpsA"
                     /locus_tag="KB953_000217"
     CDS             complement(44192..45421)
                     /gene="rpsA"
                     /locus_tag="KB953_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HBB6865223.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(45556..46236)
                     /locus_tag="KB953_000218"
     CDS             complement(45556..46236)
                     /locus_tag="KB953_000218"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBB6865224.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(46285..46920)
                     /locus_tag="KB953_000219"
     CDS             complement(46285..46920)
                     /locus_tag="KB953_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBB6865225.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(46986..48389)
                     /locus_tag="KB953_000220"
     CDS             complement(46986..48389)
                     /locus_tag="KB953_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HBB6865226.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(48376..49407)
                     /locus_tag="KB953_000221"
     CDS             complement(48376..49407)
                     /locus_tag="KB953_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6865227.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            49438..49659
                     /locus_tag="KB953_000222"
     CDS             49438..49659
                     /locus_tag="KB953_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBB6865228.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(49824..50561)
                     /locus_tag="KB953_000223"
     CDS             complement(49824..50561)
                     /locus_tag="KB953_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HBB6865229.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(50714..52054)
                     /locus_tag="KB953_000224"
     CDS             complement(50714..52054)
                     /locus_tag="KB953_000224"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HBB6865230.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(52291..53814)
                     /locus_tag="KB953_000225"
     CDS             complement(52291..53814)
                     /locus_tag="KB953_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HBB6865231.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(54192..54803)
                     /locus_tag="KB953_000226"
     CDS             complement(54192..54803)
                     /locus_tag="KB953_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBB6865232.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(54926..55051)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(55133..55849)
                     /locus_tag="KB953_000227"
     CDS             complement(55133..55849)
                     /locus_tag="KB953_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBB6865233.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(55855..56424)
                     /gene="scpB"
                     /locus_tag="KB953_000228"
     CDS             complement(55855..56424)
                     /gene="scpB"
                     /locus_tag="KB953_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HBB6865234.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(56408..57205)
                     /locus_tag="KB953_000229"
     CDS             complement(56408..57205)
                     /locus_tag="KB953_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HBB6865235.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(57175..57546)
                     /locus_tag="KB953_000230"
     CDS             complement(57175..57546)
                     /locus_tag="KB953_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBB6865236.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(57565..58452)
                     /gene="xerD"
                     /locus_tag="KB953_000231"
     CDS             complement(57565..58452)
                     /gene="xerD"
                     /locus_tag="KB953_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HBB6865237.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(58548..59021)
                     /locus_tag="KB953_000232"
     CDS             complement(58548..59021)
                     /locus_tag="KB953_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBB6865238.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(59126..59989)
                     /locus_tag="KB953_000233"
     CDS             complement(59126..59989)
                     /locus_tag="KB953_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBB6865239.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(60185..62284)
                     /locus_tag="KB953_000234"
     CDS             complement(60185..62284)
                     /locus_tag="KB953_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HBB6865240.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(62504..63613)
                     /gene="rpoD"
                     /locus_tag="KB953_000235"
     CDS             complement(62504..63613)
                     /gene="rpoD"
                     /locus_tag="KB953_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBB6865241.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(63637..65478)
                     /locus_tag="KB953_000236"
     CDS             complement(63637..65478)
                     /locus_tag="KB953_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBB6865242.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(65660..67975)
                     /locus_tag="KB953_000237"
     CDS             complement(65660..67975)
                     /locus_tag="KB953_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBB6865243.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(68160..69020)
                     /locus_tag="KB953_000238"
     CDS             complement(68160..69020)
                     /locus_tag="KB953_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HBB6865244.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(69079..69600)
                     /locus_tag="KB953_000239"
     CDS             complement(69079..69600)
                     /locus_tag="KB953_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HBB6865245.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            69878..70639
                     /locus_tag="KB953_000240"
     CDS             69878..70639
                     /locus_tag="KB953_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBB6865246.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            70665..73256
                     /gene="mprF"
                     /locus_tag="KB953_000241"
     CDS             70665..73256
                     /gene="mprF"
                     /locus_tag="KB953_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HBB6865247.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(73444..74631)
                     /gene="tuf"
                     /locus_tag="KB953_000242"
     CDS             complement(73444..74631)
                     /gene="tuf"
                     /locus_tag="KB953_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBB6865248.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            74999..75355
                     /locus_tag="KB953_000243"
     CDS             74999..75355
                     /locus_tag="KB953_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBB6865249.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT        20604 a        15901 c        12957 g        25923 t
ORIGIN      
        1 aaaagcattt tgaagcctga ctattacgac ttcataaatg cttttttagt tttaaacact
       61 tcaatctaaa agaataaccc aagattggat aaattattaa tgactcgcca ttcgtttctt
      121 agtgattctg gattatcaaa tggacttcct aatctaatca gctagttctt cccataaaat
      181 gccatacttg actgaatcat aatattgatt atttaaaaat ctaacttttc ttatcactac
      241 ttcctttgtc attccacatt tctcaccatt tttttgcatt cctttgtttc cagaccatgt
      301 agtgaatcta ataggtggta gataggcaaa atactcaaat aattctttta accataaagg
      361 aagagaaagg ctcctcctgc aagagtcagt ataatttttg cttactgctc tcttaggttt
      421 tactcactaa aactgttcca tattctttga tataagaaaa tgcatgcggt ataaatttct
      481 tgctcagctc agaaaagatt tgtcgtttaa gaaaggccat ttcctccatt gtcaatgaag
      541 gatccaatgt tggtaattca atctcaaaat tgtttttttc gacaataggt aattctaatg
      601 tgaatgattc ttcttccttt atagtattcg tcacaagtag agactgatgg aaaatactag
      661 gccggcgatt tttatattta ttcttacgta tcctgtcaac gataacttct atttgatcaa
      721 aaaagtacga ttcatttacg tctccaataa gttcggctaa ttctttgaat gccttcgtta
      781 caggaagctg attcttctgt acaaaaaagt aattagccaa agtaagcggg aagtgttcta
      841 tagaggaaag tgtgataaaa cggtacttct catttcctgc agttgtcctc agcaattctg
      901 tctctcggct gtttcttact ccataaaaat acgcttcttt ccgttcatct tcgaaacaat
      961 acgcccaaat aacttctccc atcgctaatt tttgttcatc aacagagagt ctcttcaatt
     1021 gttcagctat ggtttcagca gccgaagtgg cttgttgcaa gtaatcttta ggatcaaata
     1081 ttggaaagtt caaatgatat cttcgttctt ccctcttgat taatcctgca tcgatcaata
     1141 aatcaagaaa atgggaaaat ttattttgcg aaaattcttt tttcaattct cttagaatcg
     1201 tttcttgttc tcgatgttca tatagataat cggtcaatga agaaaaaagc ggatcatcgg
     1261 tcagtttttg ataaaagttt tccttatttt ttgaataata gttcatatat gttctctttt
     1321 ctatccgttt tattttactt aaaaggcttt tacgtaaaaa ggaggaagaa caagagtcat
     1381 aattgactgt tattctcctc catttcttcg gcctttagta tgttctatgt ttctttttat
     1441 atcaatcgct tatccattca ttttgaatgc ttcatactac catttgatta gctaaataca
     1501 gccgtcaatt gaggtacgac ttgtttttta cgtgaaacga cacctttaag caaagcacgg
     1561 ttgttggtca aagttgtttg gaaagcttct tctacttttt cttttccttt accagcaaca
     1621 agtaattctg aatcactgtc taagatgttt gtgacaacta atacgaacag atcgtagcca
     1681 tttgctttca tttcatcttc cattgctgct tctagctctg cttgtcgatc catcacttca
     1741 ttaaggtcca ctgtattgac ttgagcgata cgaacgttgg aactgcccat tgggaaactt
     1801 tttgcatcca aatcaagaag aaccgcagct gatttatcac ttagatttgt tcctgctttc
     1861 aacatttcaa gtccataagt gtttagatcg acttcagcga ttgctgcaag ttcttttgca
     1921 gctgcttcat cttcaggcgt acatgttggt gatttgaaca acaatgtatc tgaaatgata
     1981 gctgagagca tgattcctgc gatttcttta ggaattgtta cttggttttc tttatatagt
     2041 tttaaaataa tcgtacttgt acatccaact ggttctgcac gatagtacaa tggattagct
     2101 gtttcaaagt ttgcaatacg atggtggtct actacagcta aaatatttac ttcgctgata
     2161 tctgaaatac tttgttggaa ttcattatga tccacaagca tgacatctgc attttctgct
     2221 ttagctgact ctactactcg tggtgcagat agctgaaagt ggtctaatgc gtattgtgtt
     2281 tcttcattag gtgtacctag agcaactgct tctgtttctt tacctaattc attttgcaag
     2341 tgtgaaaacg caatggctgc cccgattgca tctgtatctg gattttggtg accaaaaact
     2401 aaaatttttg acatgtcatt cgctcctttg tgatttctcc tatttaataa tagcaaaaaa
     2461 acggaaagaa gcaatactta tcttctttcc gatgcgacct atcgcgttaa atattttgta
     2521 tactcttctg tatgaagcaa tcgtttagca ttttcgacac gttcttttgt aggtggttcg
     2581 atcccttcta atggatattt caaaccaagt gtttcccact tgtattttcc catcgtatga
     2641 taaggaagaa tctctactct gtctacattt gacaaggttt gaataaagtt gtctagtcgg
     2701 atcaaatatt catcataatc acttcgctca ggaactagta catggcgaat ccaaaccggt
     2761 ttgttgattt ttgacaaata ttgagccatt tctaaaatat tttcattgtc ccaatgcgtt
     2821 agttttttat gctcttcatt atcgatgtgt ttgatgtcaa ataacagtaa atctgtatac
     2881 ttcatcaatt cttgaaaacg tgaaaagaaa ggttcttcat acgtaaaagg ttttccgcat
     2941 gtatccaatg tagtatgcat cttccgctct tttgcttttt taaatagttc gatcaaaaaa
     3001 tcgatctgca atagaggttc tccaccacta acagtaatac cacctttatc tccccagtaa
     3061 ggtctgaagc gttctgcttt atctaaaagt tcatctgctg tgtattcttt ccctccgcca
     3121 atattccaag tatctggatt gtgacaaaat tggcaacgca tgcggcatcc ttgcataaac
     3181 acgacaaagc gcagtccagg tccgtctact gaaccgaaag tttcgataga atgaacatag
     3241 ccgattgttt tttcttccat ttaagttcac atcttttctt tgatttcatt ttaagtacaa
     3301 gttagctgtc gaagcgaagc tttctgaaca aaatttttcg caattgccgc ttcgttatgt
     3361 gtaaaattta cagggacgga acatacaagt cctgcccctg taaatcgttt ctttcaaata
     3421 agcatactga ttattcgatt ttgatgcgat ggctattcaa cgtcctttcg acgctaagtc
     3481 gaaaaacttt tttcacgaac tttttacatt ctgtcgtgtg aagttctcat caagacatct
     3541 aattgttgtt ctcttgtcaa gtcacggaat ttaactgcat aaccagatac ccggatcgtc
     3601 aagttcggat atttcattgg attttccatt gcatcacgca ataaatcatt actgaataca
     3661 ttgacattca aatgataagc tccttttgag aagtatccat ctaagacatt acgcaagttt
     3721 tcgattcttg tatcttgttc ttttccaaga cctgaaggat tgattgtttg ggtatttgaa
     3781 attccatcca atgcacagcc gtaattcatt ttagctgttg agttcaggct agctaataac
     3841 ccactttgtt ccgcattgta acttggattt gctcctggcg caagtggttt tccagcttca
     3901 cggccatcag gagtatttcc tgtattttta ccgtaaacaa catttgaagt aatcgtcaat
     3961 aatgaagttg ttggttttga gttgcggtaa gttttatgac gtttgatttg tcccatgaag
     4021 tattccaaga tccatgaaac taattcatct gcacggtcat catcatttcc gtatttaggg
     4081 aagtcgccat ccacttcaaa atcaactgct attccttttt cattacggat tgcttttact
     4141 tttgcatatt tgatcgctga caagctgtct gccgcatgag agatcccagc aatacctgta
     4201 gcaaatgtgc gttgcaagtc agaatccatc aaagccagct gagctgcttc ataagaatat
     4261 ttatcatgca tataatgaat gatgttcaac gtattgacat atagttctgc taaccaatcc
     4321 atcatattca tgaagttatg tttcacttct tcaaagtcta gtatttctga tgtgattgga
     4381 cgatattctg gtccaacttg ggcgccagtc acttcatcga ctccgccatt gatggcatat
     4441 aatagtgttt tagccaagtt agcacgagcg ccaaagaatt gcatgtcttt tccgacaact
     4501 gttcctgata cacagcaagc aatcgcacaa tcatcagaac cccattgttc acgaagctga
     4561 ttatcatttt cgaattgaat cgaagaactt tcgatagcga ttctgctagc atatgttctg
     4621 aatccttctg gaaggtgttc agaatacaaa acagtcaagt ttggttctgg tgaaggtccc
     4681 atatttgtta atgtatgaag cagacggaaa tcattctttg taactaatga acgaccatct
     4741 aatcccatac ctgcaatgga taaagtagcc cagataggga atcctgagaa taattggtta
     4801 tattcaggtg tacgcgcgaa tttgaccata cgtaatttca ttactaagtg gtcgatcaat
     4861 tcttgtgctt ctttttctgt gattactcca ttatccaaat cacgttggat atagatatca
     4921 aggaatgctg agatacgtcc gattgacatc gcagcaccat tttgtgattt gattgctgct
     4981 aaatatccga agtatagcca ttggacagct tcttttgcat ttgcagctgg tttagaaata
     5041 tcatagccat atttcgcagc catttctttc atttgcgcta atgcacgata ttgttcagtg
     5101 atttcttcac gtaaacgaat cacttcttca ctcattgtat gataaccagt catatcatga
     5161 tcttttttct tttgttccat caaatagtcg attccataca atgccacacg acgatagtca
     5221 ccgatgatac gtccgcgacc gtaagcgtct ggaagccctg taatgatttt gtttttacga
     5281 gcagcacgca tttcagcagt ataagcgtca aatacacctt gattatgtgt tttgcgccag
     5341 tcagtgaaaa tatgtgtcaa ttcatcgttt ggtgtatagc cattgctttt tagcgcattg
     5401 ttagccatat tgattccgcc gaacggcatg aatgcttgtt tcaaaggttt gtctgtctgt
     5461 agaccgacga ttttttccaa atctttatcc aaatagcctg catcgtgtga agtaatagtt
     5521 gatacgattt ctgtgtccat atcatagaca ccgttacgct cgaattggat atcatttaac
     5581 ttttgcaatt catcccataa tttttctgtt gcttctgttg ggccttccaa aaagctgtca
     5641 tcaccgcgat attcagtgta gttattttga ataaagtctc tagtgtctac gccttctttc
     5701 catttggtac ctttaaatcc attccattgc tccattgtat ggacctccta aatttctgtt
     5761 ttatgtaaca gtttttacgt gattagtata acacagcacc ctatctttgc aagggttttc
     5821 ttgtttttta tttaacaatt tttatgtttt atttacatat atttaaatca acgttgacat
     5881 accaacattt aagcattgtg aacactttca tctttttttg caaacgtata aggatttatt
     5941 aaaaatgaat gaaaaagaga cgccacctgt attaatacag atggcgtctc ttcattaaaa
     6001 tactacaaca gttacgactc gatgaaaatc tcaggagcat cgataacttc gatgacttct
     6061 ccaccttttt tctcatccat cgcaaatgat ccattcgatg ttcgatcacc tatagggaaa
     6121 tcagttgggt caatcgttaa ctgctgccct ttttgattca tcaaggttat ttcttttgat
     6181 cttccttccg acatatagat aatacggtgc ggattttttt tcagctcacg catgaccgtc
     6241 actccccgct tcgcacgtga agtttggctg atttcttgag caagcatgcg tttaacagct
     6301 cctcgctgag taacgattac gatcggcgta tcaccttctg ctaacacaag cagcccgttg
     6361 ataatcctat ctccatcttt caaattcata gacttcactc cagctgcctt tgctccaaca
     6421 acgggaacat ctgttaaagg atagcgcaaa cccattcctt gatgcgttac taagaaaaca
     6481 tctaagcctg gctgatcttc tttcagatac acagcaatca gttcatcttc ttccgccttt
     6541 agcttcatac agctgagcgg acggctctta taggtgcgcc atggttcgaa ctctgtcatt
     6601 cgtgtttgct tgatcaatcc attcttgctg atgaatacaa atgttttctc tgcttgaatt
     6661 ttttgatatg ggaaaacagc taaaatcgat tcgtctacag acagattgga gattgtttga
     6721 gaaatgtgct caccagcatc tttccatttc aaatcgggta gttcatggac agggcggtag
     6781 atcacatttg ctttatttgt gatcagaaga acatggtcca atgtactcaa ttcacctgag
     6841 tatagcaagt agtcgccttc tcgcatcccg atttcttctg gtttggaagc actataagaa
     6901 cgaaggcttg ttcgcttcac atagccttct cgcgtcacac tgacaattac gtcttcttgt
     6961 gccactaaga cttgcgtatc aattttgatt tcttcgattt cgtcttcgat tgtcgttaga
     7021 cgagcggaag aataattttt tttcacttct cgcaattctt ttttcataac agagaaaagc
     7081 tctttgtcat ttgaaaggat tttattcaac tctgtgattt gcgcaatcaa gctttcggat
     7141 tccttttgaa gctctgtgat atccgtgttc gtcaaacggt ataattgcaa tgtgacaatt
     7201 gcttctgctt gttcttctgt aaattgaaag acgtcgacta aattcttttt ggcgtctttt
     7261 ttgtctttac tttcacgaat cgttgcgatt acttcatcca gaatcgataa agctttcatc
     7321 aatccttcta cgatatgctg tcttttttga gctttggcta aatcgaattg actacgtttt
     7381 gttatgactt gctttcgatg ctcgatataa cttcttaaaa tgtctttcag tccaacttga
     7441 tgcggagtca tatggtcgat agcgaccata ttgaaattgt aattgatctg tagctcagtg
     7501 tttttgaaca aataattcaa aattccttga gcattggcgt cttttttcaa ttcgacgaca
     7561 atttgcagac ctgttctatc actttcatct cgtacttcag ctattccatc gattttctta
     7621 ttcaaacgga tctcatccat ttttttcacc aaggtcgctt tatttacttc atatgggatt
     7681 tctgtgatca cgatttgctg tttattaccc ttcattgcct ctatctttgt tttcgaacga
     7741 agaataactt ttcctcgtcc tgtttcataa gcttttttga tttcatcttt tccttgcaag
     7801 atccctcctg ttggaaagtc aggtccagga atatattcca tcaatttttc aaggctcgct
     7861 tgtggatgat cgatcatata gattgtgcca tcgatcactt cagctaagtt atgagtcggg
     7921 atttctgttg catatccagc agatatacct gtcgatccat tgacgagaag gtttgggtat
     7981 cgagctggaa ggactgttgg ttctttttct gtatcatcaa aattccacac gagatcgact
     8041 gtttctttct cgatatctgc tagcatctct ccacttaact tggataatct ggcttcagta
     8101 taacgcatcg cagcaggtgg atcaccgtcc atactcccgt tattcccgtg catttcgatc
     8161 agtacttccc gcaacttcca gtcttggctc atgcggacca tcgcttcata aatactgatg
     8221 tcaccatgag gatgataatt ccccatgata tttccgacag attttgctga tttacggaat
     8281 cctttgtcat acgtattgcc atctttgttc atggcaaata atatccggcg ttgcactggc
     8341 ttcaatccat cacggatatc aggcagtgca cgttcttgga taatatattt ggaataacgt
     8401 ccgaagcggt cacccatcac ttcttctaaa gttaattctt gtacttcttg tcgattttcc
     8461 atcaggtgac ctcctattct acttcaaata actgattgtt ttcattttta tctgctcgtt
     8521 cttcatctaa taaatcatta gaaactgaag gagagatttc agtatcctct tttttatcta
     8581 agatactgcc atcttcttcg agcgtaaatt gcacgtgatt ttcgatccat tttcttcgag
     8641 gttctacttt gtcacccatc agtgtggtta ctcgacgttc agcttgcgca gcatcatcga
     8701 tacggacgcg gatcaacgta cgggaagtcg gatccatcgt tgtttcccac aattgttccg
     8761 cattcatctc tccaagtcct ttatatcgct gcagcatata tcctttgccg acttttttaa
     8821 tcattgctgc caattcgtca tctgtccacg catattcaat gacttgcttc tttccttgtc
     8881 ctttggatac tttatataaa ggcggtaacg cgatatatac tttcccagct tcaatcagcg
     8941 gtttcatata acgataaaag aaagtaagca gtaatacttg aatatgggcg ccgtctgtat
     9001 ccgcatcggt catgatgatt actttgtcgt aattacagtc ttcgatagaa aactcaggac
     9061 cgactcctgc accaatcgta tagatcatcg tattgatttc ttcatttttc aagatatctt
     9121 gcattttagc tttttcggta ttgatgactt ttcctcgtaa aggtagaatt gcttggaact
     9181 ttctgtctct tccttgttta gcagatccgc cagcagaatc cccttcgact aaatacaatt
     9241 catttttctt agggtttcgg gattgtgcgg gtgtcaattt tcctgaaaga agagattcgc
     9301 cttttttacg cttcttccca tttcggcttt cttctctggc tttacgtgct gcttctctcg
     9361 cttctctggc tttgattgct ttacggacca acatctggct catttcgcta ttttcctgca
     9421 aataaaagcc catttgttca ctgatgacgt tatctactac ggtacgagca actggcgtac
     9481 caagtttttc ttttgtttgc ccttcaaatt gcagaagatt ttcaggtaca cggatagaaa
     9541 gtacagcagc taacccttct ctgaaatcac taccttccag attcttgtcc cgttctttca
     9601 gcaaaccaac ttttctcgca tattcattat aagatttcgt catcgccgct ttcataccag
     9661 cttcatgggt tcccccatct tttgtccgaa cgttattgac aaaagataaa acattttctg
     9721 aataaccgtc attgtattga taagcgacct ctacttcgat accttctttt tctcctgaaa
     9781 aataaacaac tggtgtcaat gtatcttttt cttcattcag ataatcaaca aattctttga
     9841 ttccttcttc gtaatgaaaa atttccttga ctggttcttc tccacgaaga tcagaaagag
     9901 agattttaac gccttttaga agaaaggcag attctcttaa ccgttcggct aaaatttcat
     9961 atgaaaattt cgttgtactg aatattgtat catctggtaa aaaatggaca gtcgtcccgt
    10021 ttgctttttt tgtttttcct atctttttca aagtaccgtc aggtttaccg ccgtttttaa
    10081 atttctgctg atactctacg ccatctctaa cgatcgttac aattagccat ttcgataagg
    10141 catttaccac actggctcct acaccgtgca gtccaccaga agttttgtac cctccttgac
    10201 caaactttcc gccagcatgc agcacagtaa aaatcacttc aactgttggt attccggaag
    10261 catgcattcc tacaggcatc ccacgtccgc tatctgcaac agttatacta ttgtcttcat
    10321 ggatggtcac atcaatctca ctgccataac ctgataatgc ttcgtcaact gcattatcga
    10381 cgatttcata gactaaatga tgcaagcctc ggctgtcagt ggatccaatg tacatccctg
    10441 gtctttttct aactgcctct aatccttcca atacttggat ggacgagtca ttgtattcgt
    10501 tgtttacttt ttttgccaag gcaaacgctc cttcaataat actttgcggc aaaaccgcac
    10561 cacgattatc atactagaaa aggtcgtgaa aaaaaagagg gtagacgaga aatcaatttt
    10621 aattatctcc taaccgcttt ttcgacattc ccctctatcg atcatctctt ctttgtggtc
    10681 atttttattt ttggttttct ttcaacttac caagttcaat cttaatacaa cgatccatta
    10741 cgatatctgt acgtcctgct tttcttagca tctcagctgc ttctttgttt tcaatcccta
    10801 attgtgccca gaacacttta gcatcagttt tcaaaaaatc acctgctatt tcaggtaaaa
    10861 attcactccg tctaaataca tctacgatat cgattggatc atcgatttct gtcaaattcg
    10921 catagacttt ctcgcctaga atttcttcac cagccaataa tggatttact ggtatgattt
    10981 tatatccatt gtcttgcaat acctgtgcaa ccatgtaact tgttcgttct ggtttattgc
    11041 tcaagcctac gacggcaata ttttttgctt cttccaaata gttgaaaatc gtttcttgtg
    11101 ttggattttt tacagacatt tctgccaccc cttgcttttc tttttattcc atctattata
    11161 cttgtatctt tcattttttc aattgttcct gttacctatt catgctaaaa taaaggatag
    11221 ttttcaactt gaaagagggt aaaaaatgaa attgatctta ttacttattt tagcatatct
    11281 tttaggttct attccttccg gtgtctggat tggcaaattg tttttcaaaa aagatatccg
    11341 acaacacggg agcggtaata ctggaacgac caatacattt cgtgtattag gcaaaacagc
    11401 cgggattgtt gtcctgctta tggatatttt aaaaggtaca ttggctactt gtttgccgat
    11461 gatcttccac ctttctggga ttaaccctct ttggtttggg gtatgcgcca ttcttggcca
    11521 tacatttccg atttttgctg ggttcaaagg aggaaaagct gttgcgacaa gtgcaggaat
    11581 gcttttagga tttaatcctg tattttttgt ttattcttgt atcatcttta tcgttagttt
    11641 attttgtaca agtatggtga gtttgagcag tatgatcagt gcagtattga tcactttatc
    11701 tacgatcgtc cttccttatg tagctcctgt gattttggct aagcccaatt ggttactgac
    11761 gatcattgcc tttttagttt cttcctttat cttttatcgg cataaagaca atatcaagcg
    11821 tatcagaaac catactgaaa gtcgaatccc gtttggtttg aatagcagca agaaaaaatc
    11881 ctgatacttt ttcattccaa agagataaaa cgggagtgtg acaaatcgat gtcacactcc
    11941 cttattactt acaggatagc ttttttacac accttggtaa acggtgttcc ctgctaactt
    12001 ctcggtcaca acctcttact tcactgtgat ggaatatttt gtcttgaaga tttcatgggc
    12061 acctagttga ttgatcccta ctttttccat caagtttcca ctactgttca ccgtgtcagc
    12121 gattccgcac cacggttcga tacaaacgaa aggcgcttct tttggataag gagaccagat
    12181 ccctacataa ggcatatctt tatagcttaa agttacgcta tgggaagatt tttcacttcg
    12241 aatggcgtac gcatttagac cccttgtttc aaaaaccaag gcatcttgct caaaaagttc
    12301 acgattcaaa gccagattcg tatttgtctg acctaacgtc ttttgatcca tatcgatgaa
    12361 tgggcctttc aatggtaggc gtaatcttga tttcaatgga gaaaatgcca gatagaaatc
    12421 ttcaaatgac aatccttctt caagaggtac attgaaagca ggatgtccac cgatagaaaa
    12481 gaacatttct ttttcaccgg tatttgccac ttgataatga accgtgatgc cgtctcctcc
    12541 taattcatag ctgatcacta attcaaaatc aaatggatac ttttctaatg tctccggcgt
    12601 actccttaag atgaaactag ccatttcttg tccatgttca atgacttcaa aatccatatc
    12661 ccgagcaaat ccgtgttgcc ccatttgata tgttttccct tgataaacat actgatcatc
    12721 ttttaaacgt ccaacaaaag ggaataaaac cggtgcatgt ctgccccaat acgctggatc
    12781 accttgccag atatactcta aaccatgttc ttttgatttc agactgatca attccgcacc
    12841 gtgttcagcg atttcggcga tcagctggtc attttgaata ataacagtca ttgccattcc
    12901 tccaacttcg acttactttt taaaatggga aggctggaac aaacatttct atctgcccca
    12961 gtccccttac ttctaataac tgtgttttgc taactcgtct ttaaaacggg aattcaatac
    13021 ttttaaataa gttcccttca tgcctaacga acgagattca atgattccag ctgattccaa
    13081 ttttcgtaaa gcattgacga tcacagaacg agtgatgccg atactatcgg caatattaga
    13141 agcagtcaga cgaccttcat ctccatccaa tgcttcaaag atagcttgta cagccttcaa
    13201 ttcgctataa gataatgtgt tgattgccat ttggacagcc gttgcactcc gtacatctgc
    13261 ttcgatgctt cgagacttct gataaaggat ctgcataccg acaaccgtag ctccatattc
    13321 tgctaagact agatcgtcat ctgcaaacga ttgttccata cgagataaga taatcgtacc
    13381 caaacgttcg ccagctccaa agatcggaac gacagtcgtc agaccaaatg gatatttctc
    13441 tcttgactct accggaaatg cagtaatatc actagtaatt gggatattag cttcagtttt
    13501 catcaactgt gccactgtat cagtataaga ttcaggaaat tgtttctcaa caaacattgt
    13561 tttgattcgt tcattgttca catcgtgacg ttcattataa cctagtaata ctccgtcact
    13621 tcctatgata taggtgttgc tgtcaagaat accacctagt gtgacagcca ttttatcgta
    13681 aggcaaatct gcttgcatgt caaaagtatt cttttgctgc aaaagtgtgt ttatcttgcg
    13741 ggttttcgtt agcaagtctg tcattgtcgt cctccgattt tttataaaat atagcgactt
    13801 aaatcttcat ttgccgcaat tgagccaagt ttgtcatcca catatgcttc tgtgatcgtt
    13861 atttctccca tttgcatatc tggtgcttca aataatagat cttctagtag tttttccaaa
    13921 atggtatgca gtctgcgtgc gccgatatta tctgtgtcac ggttgacatc gaaagcgatt
    13981 tgagcgatcc gttcaattgc ttcttttgta aacgtaactg acacattttc tgtaccgatc
    14041 aacgcgacgt attgttttac taaagcatta tttggttcgg tcagaatctt cacaaaatct
    14101 tcagctgtca aatcatctaa ttctacccgt atcgggaagc gtccttgaag ttctggaatt
    14161 agatcacttg gtttagacaa atggaaagct cctgaagcaa tgaataggac gtgatctgtt
    14221 tggatgatcc cgtattttgt gtttacttgt gaaccttcga caattggcaa tatgtctctt
    14281 tggacgcctt ctctggatac ttctccagat tgctgtgatt tagaagtgat cttatcgatt
    14341 tcatcgataa aaataattcc actttcttcc gctaatttga ttgcctcact atggatatca
    14401 gcatccttga ctaattttgc tgattcttcc ttgatcaata actctaaagc ttctttgact
    14461 gttaccgtcc gttccacttt tttcttcggt gtcaaagcac ctagagtttc atttaggtcg
    14521 atccccattt gctccaatcc attgttcatc attggtgcag gtttcttttg gtcttcaact
    14581 tcaatggtta cttcacgact atccagtaaa cccttttgta gttgttccaa gactgtacgg
    14641 cgattgattc gaatatcttc tgtcacttct tcttgcggtt cagcggtatt ttgctgacta
    14701 gcattaaaaa gattcatcat ttgttcaaat tgattagtgg attgtttctg ttcttttttg
    14761 ataccaggta ccaagatttt taccaaacgt ttttctgcat tttttttagc acttgtgtat
    14821 acacggctgt actgctcttt ttctacgatt tggatcgcat tttctactaa atcccgaacc
    14881 attgattcta catctcttcc gacataaccg acttcagtaa attttgttgc ttcgactttg
    14941 acaaatggtg cattgacgat ttttgccaaa cgtcgtgcga tttctgtttt accgacccct
    15001 gtaggtccga tcatcagcat attttttggt gtaatatctt gttgcatttt ttcatctaac
    15061 tgcaaacggc gatagcggtt acgcaaagca acggcaacag atttttttgc tgcgtgctgt
    15121 cctacgatat attcatctaa ttcacttacg atttgtcttg gtgtttgttt taattcatac
    15181 atatctactc acctcttaca gttcttctac gataatatta tgattagtaa agatacagat
    15241 atctgcggca atatttaacg cattttctgc aatttctttc gcactcatgt cgttttgagc
    15301 atagtttttc attgctcggg ctgcagccaa cgcaaaattc cctcctgatc cgatagctaa
    15361 gataccatca tctggtgtga taacttctcc tgtacctgaa acaagaagca tttctttatc
    15421 attcattacg atcagcatcg cttcaagttt ttgcatagat tgctgtgtcc gccattcttg
    15481 agctaattcg acagctgcgc gttgtagatt cccgttatat tcgtttagtt tgccttcaaa
    15541 tttttcttct aaagtaaacg catctgcaac acttccggcg aaacctacaa caacttctcc
    15601 attgtagata cgtcgtactt tctttgcagt gcctttcatg acaacagatt cacccattgt
    15661 tacttggcca tctccagcca tagcaaattt cccgtctttt tcgacggcac agatagtggt
    15721 tgaatgaaat ggtgattcga ccatgtttgt ttcctcctaa atttgttctt ttccttattt
    15781 ttacgctctt ggatggaaag aacgatagtt tttttgtaaa ctttcttttg ttacatgggc
    15841 atagatctgg gtagtcgata aattggcatg tcctagcaat tcttggactg tccgcaaatc
    15901 tgccccatta tttaataaat gagtagcaaa tgtatggcgc agcatgtgag gatggatatc
    15961 actatccaaa ctgctttttt tgatcatttg attcaagaca tattcaattc cagtaggcgt
    16021 gattggtttt ccatgatgat tcacaaaaac gatatcatgc tcttcgtgat attttgtcat
    16081 caatacctgg cggccttccc tgaaatattc ttgcagagca tcttgagcaa aagaacctag
    16141 aggagcatat ctctctttgt tccctttacc atggatcaac atgacctctg aatcaaaatc
    16201 aatgtctgat accgacaaac tgctacactc acttaaacgg atgcctgacc cgtaaaggac
    16261 ttccagtaaa gcgcggttcc tcaaatccaa aggactatct cctgctacgc tgtcaaaaag
    16321 tgcttgcatt tcattttcat aaaaaaaacg tggcagtctc aaatttttct ttttgagatg
    16381 aacgtaagaa aatggatttt cttttatcac ttcttctttg agaagatact gataaaacga
    16441 acgtaggctg gctatcttgc gactgataga atttctgcta tagctaggac gatgatcagc
    16501 taatgcactc aaatagacac gaaccgtaaa atattccgcc ttcagcagat cagcgtctcc
    16561 agattcctcc aaaaaatgtt tgaaatggag aatgtcttct tcatatgctt ctttcgtttt
    16621 ttctgaatat cctctttcga caatcaaata acgcaaaaat ttttgttccc acgtggcttc
    16681 tgtcattttc tcaccttcct tttgtcccag agaataattt agcacagcgt ttataaaaag
    16741 acaaataaat tgtcagaatt taaccaatat ttctaagttt gttcacaatt ggcgcaattc
    16801 ctcacatttt gtttgagaaa ttcttaagaa aaacgcaaga aaaagagcga gactatccgt
    16861 ctcaccctct tcatggagtt tccttactca tttgggattg cagttttttc taattctgtt
    16921 tttattgcct cattagcttt taaagctcgt tgtgcaattg cttcatatct tgcttttttg
    16981 tctcgaatgc gctctggcag ttcaggaaac agaccatagt tggcattcat cggttggaaa
    17041 tgttttcctt ccgcatgagt aatgtagtaa gccatacttc caatagctgt ttcttgtgga
    17101 aactcgactg gttccatttc ttttgctaag cgtgcggcgt tgattccagc aagtagtccg
    17161 ctcgctgcac tttcaacata gccttctaca ccagtcatct gacctgcaaa gaacagatct
    17221 tcgcgtttcg ttgattggta agtcggtttc agcaattctg gcgaattcat aaagctgttt
    17281 cggtgcatca caccatagcg aacgaattca gcattttcta acccaggaat catccggaaa
    17341 acacgttttt gctcgcccca tttcaagtga gtctgaaagc ccacgatgtt gtataaagaa
    17401 gctgcagcat tgtcttgacg cagctggata acagcataag gacgtttgcc agttttagga
    17461 tcttccaatc caactggttt cataggtccg aaaagcatcg ttttgaatcc tcgctgtgcc
    17521 attacttcaa ttggcataca tccttcaaaa tatttttctt tctcaaaaga tttaagttct
    17581 gccacttcgg cattgattag tgcctcatgg aaagctttga attcctcttc tgtcatcgga
    17641 cagttcaagt aagctgcttc tcctttgtca tagcgggact tcaaatatac tttatccata
    17701 tcgatcgtag ctttgtctac gattggtgca gctgcatcat agaaataaaa accatctgaa
    17761 ccattgaatg ctttgatgct ttctgctaat ggatgagaag tcaaaggtcc tgttgctact
    17821 acggtgattc cttccggtat ttcagtaatt tcttcgttta tcactgttac taatggatgc
    17881 tctttgactt ttcgggtaat tgtttcagaa aaagaatcgc ggtcaacagc taaggcacca
    17941 ccagctggca ctgctgtttc atctgccgaa gtgattacta ccgaattcaa tcggcgcatt
    18001 tcttctttca ataaccctac tgcattagcc aaactgtttc ctcttaatga attggaacat
    18061 actagttcag caaaattact tgtgtgatgc gcctcagtcg atttgactgg acgcatttca
    18121 tataaacgga cagggactcc tgcttgtgcg atctgccaag cagcttcact tccggcaagc
    18181 cctgctccaa caacatttac cattttcaat gaaagaacct cttttctatt atttttggat
    18241 gtcttcttcg taatctccat ttacacagac tacttgttta ccgcctttga ctttcttctc
    18301 gaccagatat ccgtcacatt ttggacaatt tctgcccact ggtttatccc aagaagtgaa
    18361 ttcacattct gggaaacggg aacaaccata gaatagtctg tttttcttag attttcgttc
    18421 aatgacttgt ccttcatgac atactgggca agtcacaccg atttctttta cgattggttt
    18481 cgtattccga caatcaggaa aattactgca agcataaaac ttcccatatt tccctaattt
    18541 tatcaccatc ggatgtccac agacatcaca atcgaatcct gctggttcat ctttgatttg
    18601 aattttttcg attttttctt ccgcattcgt cagttctttt tcaaacggtt tatagaaacg
    18661 atcaaccact ttgacccacg cttccgtgcc ttcttcaatt ttatccaaat ctccttccat
    18721 ttctgctgtg aaatggatat cgacgatttg agggaagaat tcacagatta atgaattgac
    18781 aatttctcct agttcagtcg gctcaaatct tttttgtgtc aattttacat agtagcgtct
    18841 ttggatcgta tcaagtgttg gtgcataagt ggaaggtcgc cctaccccgt tttcttctag
    18901 tgcacgaatc aacgtagcct cactaaaacg tgctggtggt tgagtaaaat gttgttttgg
    18961 ttcaatatca acagattgga ctacatcgcc ttcttcaagt tctggaagaa tattttcttt
    19021 ctcttctttg ccgtcatctc taccttccac atacacttgc atgaatcctt tgaatttgat
    19081 ttttgaaccg ttcgcaatga agatgacacc attttgttct aatgtcactt tcattgtatc
    19141 taaaatagct ggtgtcatct gactagccac aaaacgtgac cagatcaatg tataaagttt
    19201 caattgatct ttatctaaat attgtttgag ttcatctggt gtacgaagca cactcgttgg
    19261 acgcacagct tcatgggcat cttgtgcgcc ttgagcattt ttgatctttc ggtgatcatg
    19321 tctagaaaat tcttttccat acgtatcttc aataaatgct gcggcctctg cttttgctgt
    19381 atcggcaatc cgggtagaat cagtacgcat gtatgtaatc aagccgactg ttccttgttt
    19441 acccaaagtg attccttcat ataattgctg tgctaccatc atggtttttc ttgttcgaaa
    19501 gtttaactta cgggcagctt cttgttgcag gctacttgta gtgaaaggat tcgcagggtt
    19561 tcttttgcgc tctttctttt ctactttttt aacttcgaag tctgttccat caattctagc
    19621 agtgacttct tttacacttt gtgcatctgg taatttcttt ttcttaccgt cgattcccca
    19681 gaaatttgct ttgaatttct tgcgagcttt tttaaaatta ccgtcgatgc tccaatattc
    19741 ttctggaaca aattctcgga tctctttttc acggtcaata ataattttta aagcgacaga
    19801 ttggacacgt cctgcactta accctttctt gaccttacgc cagagaatag gactgatcga
    19861 ataaccaacg attctgtcta aggtacgacg agcttgctgt gcatctacta aatctacatc
    19921 gatcgttctt ggttccttaa aagctgcttt gactgcgtct tttgtgattt cattgaagac
    19981 cacccgattt ttttctttca gatccaaccc aagtaggtac gctaaatgcc aagcaatcgc
    20041 ttctccttct ctatccgggt cacttgccaa gtaaactttt tgagcttttt ttgctgcggc
    20101 tttcaggctt ttgatgacat cgccttttcc gcgtatagaa atataatggg gttcataatt
    20161 gttttcaaaa tcgataccca ttttactttt cggtaaatct cttatatggc caacactggc
    20221 cattacttta tagtttcgtc caagatattt ctcaattgtc ttggcttttg caggtgattc
    20281 tacaatcact aaatatttat acgccattac tgaatggact cctttccatt gtcgtgttcc
    20341 tactattaaa agaggattca tacaaatcgt agctaatcat atccattcag aaaacgtttg
    20401 tcaagaaaag caaatgaaaa gtatggtgtt attctttaaa ttctgccaag atatcttcac
    20461 ccgaaaccgt gcaaactgca ccgtcttgga tcaaatgatg acatccggta gaatgttctg
    20521 ttaaaatatt tcctggaaca gaaaaaactg ttcttccaag atccaatgct tgctgtgctg
    20581 ttatcagaga gccgcttttt ttcttggctt caaagacaca tgttccttta gacaaagctg
    20641 cgataatccg atttctcatt ggaaaacaat gctttttcgg gccggattga ggaggatatt
    20701 cactcaaaag aagatgatca tttgcaatca cctcttgcaa gtcacgattt tcagcaggat
    20761 agacttgctc tatccctgtt ccaatgactg cgattgtccg tccaccatga gagattgctg
    20821 cttggtgacc gtatgtatct atccctttcg cgcagccact gacgatcaca tatccagctt
    20881 ctatcactgg ttttagtaaa taataaactg tttttttggc taaagcagaa gtgtgtcttc
    20941 caccaacaac gcttaacatg ttacaagaaa gtaaagatag atcaccatta taaaacagta
    21001 caattggcgg ataggtcaag tgaagcagct gagggggata tttgggatca aagcaagtga
    21061 taaacgactg tttcgattgg attttgcccc agttagcgtg aatttcctgc caacttttgc
    21121 gaaacagctc ccggtgtttg gttattttac ctatcttaat gatgtcttca aatccgattt
    21181 ctttataagg atactgcatc atccagttag caagtgccca ttttccttga ctactgattc
    21241 cttttgtata agcaagtttc aataaatttt cttctatttg atacatcata aaaccgcctt
    21301 tcgtctctct ttctaataag atacgtaaaa gcggctgttt tttattgtaa atattttttt
    21361 acaggtgcaa aacttttacg atggattggc gtaattcctt gtcgttcaag tccttctaag
    21421 tgttcctttg ttccataacc tgcattatgc gagaaaccat atccaggata aagcacgtca
    21481 tattctttca tcaattggtc tcgatatact ttagcaatga tacttgcagc agcaatcgac
    21541 acagatcgtg catccccttt gattattttt tcctgctgaa taggcaaatc caaggtcatc
    21601 gcatcgatca acaaatagct tggtacaatg gacaattttt ctactgcttg gatcatcgct
    21661 aatttacttg cttcataaat attcacatca tcgattgtct gctgatcaac gatcccgatc
    21721 ccgattgcca gggctttttc ttgaacttgt tgataaagaa gttctcgctt tttctctgat
    21781 aattgtttgg agtcattcag ccctaatatg atctcatttt tcggcaagat aactgcagct
    21841 gccacgacag gtccagctaa tggtcctctt cctacttcat caatgccggc aatccattgg
    21901 tgaccttgtg cgtacccttg cttttcaaag caaaacattt cttcatattt ttccttaatt
    21961 aatgcttcct ttttcaatcg tcgttcaaaa gaagccagtg cttgctgcac gcctttgcgt
    22021 ggatcatttc ttaatgattc tagtgtagga tcatctattc tctgtatgct tttcaatcgt
    22081 tctttgatac tttggatgga ttcagtcatc acttttttgc tccatttctt cgcaacgatc
    22141 cagtgtgtat cgtccaagtt ttccttgacg aatatcatgc acgactaatt cgctgccacg
    22201 atcatagtct tctttatacc cacgttttgc tgtaatatcc atcagtaatt ccggtgccaa
    22261 tttttcttct tgctcgattg ataaaccata acgatcgctg attcttcctg aataatatcg
    22321 ggcaaaaaag tctaatccgt aaatcgctaa atcatctaaa tggacaagct ggtctttgat
    22381 cgcgcctgtc agcgctagtt ttttgccgat ttcttcatct tcaaatttgg gccataaaat
    22441 ccctggtgtg tctaacaatt ccaactcatt acctaaacga agccactgct gaccttttgt
    22501 tacgccaggc ttatttcctg tctgtgcaat tttcttgcct actagtcgat tcatcaacgt
    22561 agatttccca acatttggaa tacctaagac cattgcccga atagcgcgag gcttgatccc
    22621 ccgagcagca tctcttgccc gtttttcttt caatgcttct ttagccgcag gtactagacc
    22681 tttaattcct ttactttctt gtgccgtgat agctaatgca agatggcctt gtttacgaaa
    22741 atattcttgc cacagctttg tctgttgtgg atctgccaga tccgctttat tcaataaaac
    22801 aaggcgaggt ttttgctgaa tgatctgatc tagcattgga ttacgagaag ataaaggtaa
    22861 tcgtgcgtct accaattcaa atacgatgtc cacatatttc attttttcag aaacttctcg
    22921 tcgggctttt gccatatgtc ccggaaacca ttggatggtc attgttctct ccattccttt
    22981 cataccggaa tattccccgg ctttacttgt ttttttcttt ttcaatataa attcactttt
    23041 ttattataaa gaaattccat ctattagtta agtgaaagtg aaccagtcct cattctatca
    23101 catcttcact tttttgatga tgcaaatttt tattttggtt tctatatatg aatctaaatt
    23161 tttgacagcg tttcctaaat aagagatgat agacgaacaa aacagaaagg agacgaaaca
    23221 aatgaatagt atggtttttg ttaattttcc tgttgctgat gtcaatcgtt ccgttgcttt
    23281 ttatgaaaag ctgggtttta aaaaaaatga agaattttct actgaagaag ctggttcaat
    23341 ggtatgggat gacacttttt ggataatgct tctctctcat gatttttata aaaaatttat
    23401 aaaagataaa gaaatagctg ataataaaaa aacaagtggt gctttgattt cgtttagtgt
    23461 tgaaaatgcg gatgaagtaa aaaaatttgc tcaaattgca aaagaaaatg gtggtacttt
    23521 ccacgaagta gatatgggaa tgcctgaaga agaaatgttt tcattagaag tccaagatct
    23581 agacggaaat acattagaac ccgtatggat gaagatgtaa gcagataact aatctgacgt
    23641 tttttataac aaaaaaacaa aggcggatga tacgataagt attctccgcc tctatttttt
    23701 ttattcgtac aatctctttt ttacagttta taaaagcagt cttctcaccc tttttttaat
    23761 tgttagatgc cagtgtatgt aaatcaccaa tgtgtcatga cacattggtg attttatggt
    23821 atccttttat tattaactga caaaggagag aatattttga acactttgat tgcaaaagac
    23881 gtttttagat tgcccgaaat gaatgcaaga ttgttgtcga cctctgctct tttattagcc
    23941 cttgatgtca tcttatacaa gatagcgatt ggtcctgctt attttcaaat cacattcgga
    24001 tttttaagta tggcgcttat gggttatcta tatggaccga tctgggctgg tttgcttgaa
    24061 atgttgtcga acactttaaa tttcacgata tttggttcag gaacgtttca agctgctttt
    24121 cttatcacgg ctttccttgg tggatttatt aatggcttat ttttatatca taaaaagata
    24181 aacctgcctt ccgtcatcgc tgcccaatta ttgattatga tcccagtcag cctttttatc
    24241 aattcttggc tgatgtctgt tttgtttggt gcagatttca aagtaatttt tctaggtaga
    24301 ttcatcagga atatgatcca aatccctatt caaataatgg ttgtttattc atttatcaaa
    24361 actttagagg ctagaaaagt gtttaaacga gctgtttgat tttacgtact aaggtagtaa
    24421 actgattttg gattaaacaa taaagcgtgc agtctcaaca gactgcacgc tttattgttt
    24481 ctcactggat ccattttatt tcatcaagtg gataatagac aaatcttgct ttacctagaa
    24541 tatcagtttt attgatcgtg ccaaacgaac gactatcttt tgaaactctt cgattatctc
    24601 caagtacaaa ataattatct tccggaagct tggattgtcc cgttagctct tgtagtgtaa
    24661 agtccgtcgt ataggatgca tttgcatgat ctgatttcag gtttttagtt agataaggtt
    24721 ccttaactac tttcccattg atcttcaatt gatcattttg gtagctgaca ttttctcctg
    24781 gaagaccgat tactcgtttg atataaatcg tgccatctgc taattgaaaa acaacaatat
    24841 caaatcgtct gatctccgaa aatttttcca tcacaaccat atcaccttgc ttcaacacgt
    24901 tttccattga attgccttcc actggtaccg ggatgaataa aaatccccgc aacatgaacg
    24961 caaggaatac agaaacaagt aagtatttga atacaagcca gaagcgatta atatagcgtt
    25021 gtttttttgt caatctctat caatccatcc ctcttttttt cattctttct ttattataag
    25081 aaaaatatca aaaaaatgcg actagatagc tgaaataaaa ataattcttt caattaggct
    25141 attggacaaa aaactttttt gatcataaag tacaaaacta tctcttatat gtcatgatta
    25201 ttttctaaat atttttccca tgcttcgtca aagatatcca tggacggcaa atagtcgaca
    25261 ttcagttcca gataattaga aatttcttcg tagctagagc tgtgcttagg aaattggatg
    25321 tctttaccgg cttctgtagc gaaaacagat aaagcatcat gtgtgccacc gcctcgcagt
    25381 gtcattaaat aatgaaaaaa gcttcgattc acccttcgtt cctccaattt tcttcgataa
    25441 aggcagcccg gcgtgcatgg tttcttttaa aattttcagg atgcttttta taatactctt
    25501 gatggtcttc ttctgctaga taaaatggtt gggctggttc gatttttgta acgattggct
    25561 gttcgaaacg tccactttca gctaactgtt tcttactttt ttcagcaatg atccgctgtt
    25621 cttctgtcaa ataaaagatc accgggcgat aattgtcacc gcgatcctcg aattgtccga
    25681 aagcgtcagt tggatcagtt tgctgccagt aaagttctac taattgttca tatgaaatca
    25741 atgttggatc atagataatc tccactgctt ctgtatgtcc agtcgtttgg ctttttactt
    25801 gttcataagt cggatctggt acatgccctc cagtatatcc tgacatcact gtatggatac
    25861 caggaagcgt gtcgaaaggc tggatcatac accagaagca gcctcctgca aatattgctc
    25921 gttccattat gattccccct ctaatactac tcatccgatg attttttatt ttcttttgtc
    25981 ttgctttcag tactgctggt gctagttttg ttggtatttg agttgtttga agcatcagag
    26041 gtatcttttg gcaaatatag actaaaccga atgtcatcat ttactaagtc gattttatct
    26101 gctttaatat acatcccgtt ttgcattcgg aattgatcca acctaagcat gatagtcaga
    26161 tcatctgtat tgacttcgat ccaatctggt aatttatagt ttctcttgat catattcatc
    26221 acatctttga ttggcaaact caatgttccg atagaaagac ttctagcttt tagttggaca
    26281 tttccgtttt ccatcacata cggatcaaaa tataaataga aatcgatagg aaaacctagt
    26341 acttcgaaag taccattgag tagtgcttca ttttccaaat aaaatttata ttcaatatca
    26401 ctatcttttt gatactcact aaggaaaaag ctgatgattt gattgacttt ttctttattt
    26461 gaattgatcg ttaagacagg atctccttgt ctttccgtaa gagctggtat ttctgggagt
    26521 tctggttccc gattagcaaa aatacggctg cctaaaaata cggtcgaacc aagtactacc
    26581 ccaagaagga taaggaaggc gaatttccag taatttgttt tctttgtttt gtttgaagat
    26641 acgtcagatc ttgttttcat gtttattgat catcctcctt ctctaaccat tggttttgtg
    26701 tctcgatcag ctgatctctc actgcatttg ccatcaattg ataacctaaa ttatttggat
    26761 ggaaatgatc ttcttcatag agcacattat ttttgatgcc atttagattt tctgtcgtgc
    26821 tagttgtttg atctgttcct tcttctgtga cacctacttc ctgattcaat ccttgataaa
    26881 gcaaatcatt gattgggata aagtagcagt tcggcatttt ttttgtcacc gtttctgttc
    26941 ctttgttcca attgttaaca attgtctgca tgtcagtgat ctcaggaaag ttcaaataaa
    27001 atggattgta gatgcctaaa atgtaaatag gtgcggtttt attgagggat cgaaattctt
    27061 ccaatagttc tgtgacattt tcctgatagt ttttcagtgg ctttttaaag gaattgaccg
    27121 ttaacccgaa taaattattt tggataactt tcatcaaatc attaccgcca acagtcagtg
    27181 tgataaaatc agctgttttg atattgtttc gaatttcttc ttttgttttt actcgcttta
    27241 agatctggtc acttcgttca ccatttacac catagttttc tgtttcaacc gcggtcaaat
    27301 tataacggtc ttttagatca tttgcgacaa ggggaacgaa tcctccttgc ttggtttgat
    27361 ctcctactcc ttcagtcagc gaatctccga tagctatgta gtgaagtttt tcttttttat
    27421 ttggatcttc tctcacagta gttactggtt tcattagtgg gccagctttc ggtatagaag
    27481 cagctaaacc tgcatagatg atgatcgcaa ttaataatgg gaacaaaacg aggatcaaat
    27541 gtcgtttcgt taatttcatt ttttcacctc ttgtattcga ttggttctaa tgttatcaaa
    27601 aattttatca aaaaggtagt ctttatgaaa aaagaaaaca attattttcc taattttata
    27661 aagaaaaaga gaataagctg caagcctttc aggctgaggg cttatcctct tttcgactaa
    27721 tctacataat acatgattgc aaacgcgtct ttaccagtat gcgtagcgat aaccggattc
    27781 gtatgcagta cagggatatg catgtccggg aataattctt gcaaaccctc tttgaattct
    27841 tgcgccaatt cttttgcatc cgcatgtgaa ataccgattt gtcgcacatt tggcaattct
    27901 ttcagttcat ttttcaaatt attaaaccat ttatcgaacg ttttctttcc tcgcccctta
    27961 gttacaggga ttaattcaga atgggcgaaa ttcatcacca cgcgtatgtt caaaacacta
    28021 gacagcaaac cagttgttcg gctgattctt ccacctttta ccaagttatc tagtgtggat
    28081 attccgatgt ataattttgt attgtcttgt acttttttga cttcttcaag gattttctct
    28141 actgttttac cttcttttgc taattcagct gctcgaatta cctgaaaaga taatccttgg
    28201 tcagtggtat cactgtcgat aaccgttact ttcgtttttg ttagattact ggcttgtctt
    28261 gctgcttcta ctgttccact caagcctttt gtcatatgga tcgaaatcac ttcactgccg
    28321 tcttctccta gctcatcata catcgagatg aattctccaa ttggtggttg gcttgttttg
    28381 ggcagatttt ttgctttcgc catcatttcc ataaatcttt cgccgtctag ttggtcatca
    28441 tcgggatata ctgttccgtc gatcatgatg gatagcggca tgacacgaat gcctaaagca
    28501 tcgcgtacat ttttttccat cgtacatgaa gaatctgtta cgatttttac atttgtcatt
    28561 attttctctc tctctttctt taacaagatg ccttattcat gttaaactac atgtaagaca
    28621 atttctattt ttagtataac agacaaataa tgatttttca ttaattacac ctagtaaagg
    28681 agccgttttc atggaaaaaa cgaaatttaa tcgaacgtat gaaatattaa atgaagtgtt
    28741 taatgcagtc acccatggaa ttgggactgg attgagtatc gcgggactag tggttctttt
    28801 agtaaaaggt gcccacttgc attcctccct ccatattgtc tcttatgcta tctatggatc
    28861 gatgatgatt cttttatttt taacgtctac tttgtttcat tcattgattt ttacaaaagc
    28921 aaaaaaggtg ttccaagtat tcgatcatag ttcgattttt cttttgattg caggaagtta
    28981 tactcctttt tgtttgctga gtatccgagg atggctaggt tggactttat ttgtattaat
    29041 ctggttgttt gcgattcttg gcattatata taaagcgttg acgttgcata aacgggagag
    29101 agtatcaaaa gtatctacga tcatctatat ttttatgggg tggctctgtg tcgtggctgc
    29161 tgttcctctt tatcactctc ttggaatgac tggtgttatt ttgatggtag caggcgggct
    29221 ttcttacacg ataggcgcat tcttttatag cttgaattct atccgctata tgcatgttgt
    29281 ttggcatttg tttgttatgt taggtgcagg ttttatgtac ttctctgttt tattctttac
    29341 ttaataaaaa aaataaagct atggatggga cagctctaaa taacagctag tctatccata
    29401 gcttttttat atcgtcaatt cgcctgtccc gctttttaca ggattctcat tacgctgata
    29461 cgtttcaaat tgatgcggat aacgattttt ttcatccatt tctccttgac tagtattgat
    29521 cagtgaccaa tcagaccagt caacaggtgg aaaataagca tctccatcga atacatgatg
    29581 gatgactgta cggtataata tcgtacaaaa tggcagaaat tcttgataaa cagctgaccc
    29641 tccagcgatg aaaaagatac cgtctgtttc tttttccaga gcaagcacgt cttccacact
    29701 atgcatgaca tgcacgcctt ctgctttata ggaagaatct ctcgtcaaaa caatcgtctg
    29761 acgattcggc aacggacggc tacccatccc ttcgaatgtt tttcgtccca tcactagtgt
    29821 atttgcttct gtcatttgtt tgaagaattt cagatcattt ggcaaacgcc aaggaagacg
    29881 gtcttctttt ccaatcaatc cgttttcatc ctgtgcccaa attgcagcta acattttctc
    29941 atcctttctg attctaaaca gcgataggag ctttgatcgc cggatgtggg tcatatcctt
    30001 ctaattggat atcttccatc tcaaaatcaa atactgactg cttttcctga ttcaaaacta
    30061 aagtcggaaa tgcgcgtatt tctcggctca attgttcttt catttgttcg atatgattga
    30121 tataaagatg agcatctcct aatgtgtgaa caaattcgcc aaccgctaaa cctgtttcat
    30181 gtgcaattag gtgagtcagc aacgcataac ttgcaatgtt gaacggaacg cctagaaaca
    30241 catccccact gcgttgatac aattggcagc ttagccgtcc ctcttgaaca taaaattgaa
    30301 acatcgtatg acaaggaggc aaagccatgc taggaacatc ttccggattc catgcagaaa
    30361 cgatcaatct tctagaatca ggagtctttt tgattgcttc gatcacttgt ttgatttgat
    30421 cgataaagcc accgtctttt gtttcccagt gacgccattg atagccgtag atgtgtccaa
    30481 gatcgccgta tgtttcagca aaggaatcat cggttaggat cttctcgcaa aaaagcttgc
    30541 tctgcttttt atattcttca ttgaattcag gatctgtcaa acatctatgg ccaaaatcag
    30601 tcatatctgg tccattatag tctggacttt tgatgtagcg ttcaaatgcc cattcatccc
    30661 aaatatggtt tttattttgt aatagatagc ggatattcgt atctcctttt aaaaaccaaa
    30721 gcaattcgct tttgatcaat ccaaaaggta ctcgtttcgt tgttaacaaa ggaaaccctt
    30781 tttgcagatc aaaccgcatt tggtatccga ataagcttaa tgtgcctgtg cctgttctgt
    30841 cttctttacg atgcccttct tccaacaatt ttcttcctaa gtcaagatac gcttgttcca
    30901 tcctttttcc tcctaatctt ctacccattc actcaagtat tcccaccgtg ccattttttc
    30961 ttcaagttgt gcttctgttt cagatacgtc gttttgcaat tcttgtaatc gagtgaaatt
    31021 atctccttga tgattcatct cttcttggag caatgagatt ttttcttcaa gttctgcaat
    31081 ctcatcttca atcgtttccc attctttttg ttccatgtaa gataatttct tcttttcagc
    31141 aggttccttg ctctgtattt ttggtttcgc tttttcagca ggctgctggg tctctttttg
    31201 cttcagtaaa taactggaca tgtttccgta ataaagttct tgttttccgt ttccttggaa
    31261 tactaataac ttatctgcta ccttgtccaa gaaataacgg tcatgggaaa cagtgatcac
    31321 agcacctgga aaacttctaa aataatcttc caaaatcgtc aatgttgcta tgtccaaatc
    31381 attggtcggt tcatccaata ataaaacatt aggttgctga atcagcagtt tcaacaaaaa
    31441 taaccggcgt ttttctccgc ctgaaagttt gcctatcagt gtcccatgca taaatcttgg
    31501 aaataaaaat cgttcaagta attctgctac tccgatttga ctgccatctg ctgtttttgc
    31561 ttcttcagca gcttcttgta agtaagcaat catccgttta tctggtgcca tttcttcatt
    31621 ttcttgtgta taatacgcaa gatgaaccgt ttcaccgata gacattgtac cgctatccag
    31681 gggaattctg ccagctagaa tattaagcaa tgtcgatttt cccgcaccat ttttaccagt
    31741 aatacctagt ctttcccgag attgaatcaa taaatctaaa tgttctagca gtgtttggtc
    31801 atttatacgg taactgccat ctttgatttc cagtactttt ttccctaaac gttgtgtcgc
    31861 taggttcagc tctaaatcat cttcttggtt tacttggtac aagttctcct ttagatcttc
    31921 gaaacggttg atccgtgctt gctgcttggt gccacgtgcc tgtacgccag ccctcatcca
    31981 agctaattcc tgtttataaa gccgcttgcg cttttcttct tgttctactt ctacacgatc
    32041 acgttctgct ttttctaaga cgtaagcttc ataatttcct ttatactcgt ataattttcc
    32101 aaatgaaagt tcgaagattc ggtttgctac tctatctaga aaataacgat cgtgggtaac
    32161 catcaatagt gcgccacggt attgtttcaa atagttttcc aaccactcga tcgcttcata
    32221 atctaaatgg tttgttggtt cgtctagtaa tagtaaatcg ggttcatcaa tcaacacttg
    32281 cgccagacta actcgttttt tctgaccacc tgataaagta ctgatttttt tatctaattc
    32341 actgattcct aatttttgca aaataatctt tgcgttggta tctgtagtcc aagcatcttc
    32401 tttattcatt tgttcttctg ctttagcata acgtttttgc acatcagggt cttctccgtt
    32461 ttcccctagt tcgatcaatg cttcctcata tgctcgaaca gtttggatca aggggctgtt
    32521 tccttgaaag actgcttgga gaacagttaa ttcatcggaa aattcagctg cttgagaaag
    32581 ataaccaata cggtaatcag ttggataaaa cactgtttgc cgatcaccgt cgctgctatc
    32641 gataccggca ataatattta agagacttgt tttacccgtc ccgttcgtcc caatcaaccc
    32701 gatacggtct ttttcgtgga ccaaaaaaga aatctggtca aacaaatctt tttcaccata
    32761 agtttttttg aggtcagtta cttttaattc tttcaacatg ttccctcctt atcttggata
    32821 ttgtatcata aaaatagtga acttcaggaa aatttttttg gtatttgttc tttttgtgtt
    32881 ctcaaaaaaa gaacaggacc gattcgatcc tgttctgata tgttttgttt tttataaaac
    32941 ttgaaaattt ttaccttttt attttattgg actcgttttt tttgactttg ctcgaaacgc
    33001 aaatcggaaa gaaaagttat cagatggttt gttcatcgta taagattcta acaaattgat
    33061 attcctgcga tagctgttcg cagaaaacgt cgtgattttt tcagcaactt cgtattcatc
    33121 aatgattttt ctttcaagat aataacctct tagcattttt ttcgtaaaca gactatcttg
    33181 ctggatcatc cacgaagaaa tcaaattccc cgagttcatt tttattcctt caagtttgcg
    33241 ttgctggata aaaaattgaa ccatcgtttc atcataaata tcagtcaagt tatctaaact
    33301 ttcaataatc aactgagtat ttttttgata tgtctctctg atttcttgga tatcttcatc
    33361 actaataaat gtctgacgat cagcatatct tctttgccaa aacattttcg gatggagaac
    33421 gattcgcatt aacctacgga caacaatgaa ccaaaagccg ataaaagaca aaatctggct
    33481 tgtgatagaa tgttcgaatc gagaaagaaa acgggagtaa aaacgataac cattagaaga
    33541 aagcttgccc gaacgataac tttcatccaa tccatcccgt tcgatcgaca aaatcagtgc
    33601 ttgtatttct tgtacttctt gcttttcatc atcggttaaa tcttccaagt acagatcacg
    33661 tatccgttct tgataatttt ctatcactgc ttcagtggct aaaaattctt ttgtattacg
    33721 atcaatttcc tttatttctt ttcgcaaaac ttcaacgacc tcttctaaaa tcatcaaagc
    33781 attcaaatcc gtgcttttcg cttcctctgt ttccgtcagc ataggcaaga caatgatccc
    33841 tatgaccaaa gtcactaaga tgacacccgc cgtcagaaac aaaagcaaag accgctggta
    33901 aaacatcata ttattgattg aaaatggcaa aataaaaatc gtagccaaac tgactgttcc
    33961 ctttacaccg ccaaatgtta agatcaagat ctcgttcaac tgttttttga actttttact
    34021 gccatctttg aatacataaa aaagactgat agaaatgaat ctgatcacaa aaagcatgac
    34081 agagattaat acgataatcg ctaataaaag accgtttgga tataaaccgt ctccccatac
    34141 tggcgagaac acttgcgtca attcgattcc taaaaatata aatactaatg cattcagtgt
    34201 aaaagcaata gttgtccatg tactgtggga caagctcgac aattctgcat caaacacaga
    34261 gattttccta aaaccagaag cttggagaat acctgctgcc actgcagcaa tgattccaga
    34321 tacatcaaat gcctctgcca atacataggc tagaaaagga agcaaaagtt cgatcagcag
    34381 ataccctgtg acatcttgtg ctgatgcttt ttcgatcaaa tgaattattt tttgtttgaa
    34441 ccagacaagc aaaaagccga tcaaagctcc cccaatacta gaaactacta gactcatccc
    34501 cgcgtttact gctgaaaaag atccggtgat cagcgcacca agagcaaact ggaaggcagt
    34561 aacaccagat gcatcattca aaagaccttc tccttccaaa atatgcatcg cctttttagg
    34621 gatattcact cgtccagaaa gagaactgac tgctaccgca tctgtcggac ctaacgctgc
    34681 tccaaaagca aagcaggcag caagtgggac acttggcaac aggaatgata acgtcgcacc
    34741 taccccgaca agggtcaaaa tgactcctcc aaatgccaag aacaaaattg tcccgaaatt
    34801 tttgagaatc gaagaaatat ctgctttttc tccttctcta aataacaatg gtgcaataat
    34861 cataactaag aacaattcag gttcaaaatc gattgattgt ccccactctg tcagacctaa
    34921 taccactcca aggaaaattt ggatcagtgg agctggaatc attggcaaaa tgcggctagc
    34981 tatgttcgat aaggtgatcg ttactgcaaa tacaataact aactcagcaa attccatcgt
    35041 ccatcccctt tattttttat tttgtgtaag catttttaaa aattgttctt ttttctttaa
    35101 aattttggtt tcgactactt caacctgtgt ttggttcccc tctaattcac actgtacacg
    35161 ttttgactcc aaacgaagca gatcatgttt cagacgttcc aactgacgat acatcaatat
    35221 atttctttta cgcaacttac aaagtatttg acggcggtca tccatcgaaa gggcgccata
    35281 gcgatcatgc aacagttttt tttgttgttc aaaactctct ctcattttcc agatctccta
    35341 tacacaaact tccatcatta tagcaaagtt ctttttggat gaaaaatatt tcattgtttc
    35401 atcgtttatt gctagttaga aatttccata aatttttcaa cgcctcattc tattccaaat
    35461 agtatcagat atcaagtaaa acttaagaaa gataaaagtt tctgacaaaa ggatgtttac
    35521 tttcccattc ctgcctttct tgttctttta tcacaacaaa aaaatggggc tgcgacaaaa
    35581 atcttgtcac agtcccttat tctgaataaa cggtgtggaa gagacagcct atcagtagta
    35641 ataagacaca tctctttcac agcctcattt ttgcgtaaaa atcgatttat tcgaaaaatt
    35701 ccccgtatac atcttctaca cgttgatctg gattgatcac gatatacaag ttacctgttt
    35761 tttcgctgat ttttgttgat tgatattgat tgtctaatcc ttctgttcct ttatctagat
    35821 atgggaagta cacaagatca ggtgtgccat ttcggtaaag gatttccatt cgctcgatat
    35881 cttcgtattt caatgcgcga ttaaacatgc ccatttccag tccgcctagg ttgatatcat
    35941 gtgtcgcaac tgcgtcccct tcatggaaga tttcgatttt aaatccagca cacggatgga
    36001 tttcaacaaa ttcactgcca ttgattcgtc caaaacttgt tgtgatttgt ttgatccaaa
    36061 gatcgccgat atgacggcgg ttgatcgtcc aggtttctcc atttctaaaa taaaatttta
    36121 gcgctgtcat ttctttactc attaaaaaga ctcctttatg atgtaattta ttcttgtact
    36181 acctcagttt agcacaaatt tatgtaagtg gtcacatctt atgttttttc ttctaatgac
    36241 gtatctgccc actttaagta gccaacaaag ctgagcagat acagtctagt gttttttaaa
    36301 tcaatttttc tttcgataaa aaatccaaaa cctgtttttt atcatttttt attctttgtt
    36361 ctacgacggc tttttcagct aattgcaaag cttcactaac ccacttacca ggtttttttc
    36421 cagtttcttc tagcaagatt ttaccatcta ctgctaattc gcttaatgac ttgataggta
    36481 aacgatcata ggattctcct acggcatcca tctgaggtgt ttcgtcaaaa tagatcatca
    36541 attcttctac ttgcagtgca tgttttttgc ctaagaagta caattcgaat ggttccaaag
    36601 ctccagtttc tcgttgtcgt agtccttgga caagtgcttg aacgtcttta atcatttggt
    36661 tagaacactt ccacgatttc aaaaaggaac gaatctcatt ttcttttaga tccagttggc
    36721 tgatcaataa tgcccatgct tggctctctt ctttcaatgg gacggatggt aattcagcaa
    36781 aacgtaaaag tgcttcgcct tcttctttca aaccgggaca gcaaatgtag cactctgttt
    36841 caataaatgc atggattccc gcggcccgtt catttcccaa gagcatcttg atgaattcca
    36901 catttatacg ttctacagat attttaccta atagctcgtg gttttctttg atggcgtaga
    36961 aggtttcttc ctctaatttg aaccctagtt gactgacaaa acgcaacccg cgcatcattc
    37021 gtaaggcatc ttcatggaac cgctcatgag gattgcctac agctcgcaag atctgttctt
    37081 ctagatcttg cagaccatcg aacaaatcga tgatttctcc atcttctttc aaagcaaacg
    37141 cattgatcgt aaaatctctt cttttcaaat cttctgccaa cgaacgaacg aattcgacat
    37201 gatctggtct acggtagtcc tgataagttg attccgtccg aaatgtggtg atctcatatt
    37261 gttcatcatt ttctaatacc aacacagttc catgttcgat cccaatatct atcgtttttg
    37321 gaaaaagctg tttgatttct tctggaaatg cgcttgttgc gatatccaca tcatggattg
    37381 gctggttcag caagatatca cgaacacttc cacctacaaa ataagcttca tatcctgcat
    37441 tctctaattt tttcagaact ggaattgcct tttggtattc cagaggtaat tgttttaatt
    37501 tcatttttcc aacctttcct gcgtgtttct tgtcctgaga taaagactca gttgcatggt
    37561 gactgtttga caagtccgtt tcagagtctt caaggccagg accacttcat cttgtttact
    37621 cgtccttacg ctcaaagcga ttatggtctc tttgattgaa tttttccatg tttttttcaa
    37681 aaacttcagt taagtcgata ttcaatgaat tagccatgat catcgtaacg aataaaacat
    37741 caccaagttc ttctgctacc gagttaactg gttcagaagc ctttttgctt ttttctccat
    37801 aagtgtggtt gacttctctt gccaattcac ctacttcttc agttaatcga gccatctgag
    37861 ctaatggaga gaaatacccc accttgaatt gctggatata ttcatccact tcctgctgca
    37921 tactgtataa agaacgcttt ttatccatct ttttccctcc aaaactatct taccaaaact
    37981 ttaacgcaaa tcctagataa gagcaattga gaaaatcgaa tttccatgct acatttagga
    38041 aggaatttct aaatggaaat tctgtgaaaa tacataattg aaagctggaa ttcatatggt
    38101 caaacgaaaa acagtaaaag aagttctttt tatcattata ggaacaagta tttatgcatt
    38161 tggtcttgtt tacttgaaca tcgctaatca tttagcagaa gggggagtct cagggatcac
    38221 cttgatctta agagctttat ttggtattga tccagcttat acgactttat taatcaacat
    38281 cccattgatt ttactaggag gaaagatcct cggtaaacgt tccttggcct atactgttct
    38341 aggtacactt tctttgtcat tctttttatg gatctggcaa agggttccac tagaaatcaa
    38401 tttacagcat gatctactga tcgtttctct acttgcgggt ttagttgcag gtgtaggaag
    38461 cgggatcgtc taccgcatgg gtggaacaac aggcggcagt gatattatcg ctcggatttt
    38521 agaacaaaat tacggcatca ctatggggcg ttccctattg gcatttgata tcatcgtctt
    38581 acttgcttca ttaacctata tcgacttgaa acgtatgatg tatacgttga ttgtttcgta
    38641 tgttttcagt cgtgtgattg attcgatctt agatggcgga tattctgcaa aaggtctact
    38701 tgtcgtctcc aacaaaagcg aagagattgc accactactg atgaccggtc tgcaaagagg
    38761 tgtgactttc ctttctggag aaggcgcatt ctctggtaat tcaaaaaaaa tcatctatat
    38821 ggtcgttagc tctagtgaat taactgaagc taagaggatc gtccacgaga ttgacgaaaa
    38881 agctttcctt tcaatcatca atgtgcatga agtggaaggg caaggcttta cttatttgaa
    38941 gccacagaca agattactga aaaaactata agaaaaaaga caaagatagg ctatgaggaa
    39001 agattccaca tccagctgac ctttgtcttt tttagtacta tactattttt aactaattgt
    39061 tatgttcact tacccggtta cttttctttt gtgtctgcaa aaggatcttt cggttaactt
    39121 catcagataa tttcaaaaaa gcatccctat ctccatcttc tagtgcttga tcgatttgag
    39181 cataaagcat ttttagtttt gcctcttctt ctttttcttg aaaatatcgt tcaattaatt
    39241 cagtatcttc ttcactgact gaagaatccc aagaagaaaa tggattgtct tctaagatag
    39301 atagatattc tcttgtctgc caagcatttt cgaatataca ttccacatat aaaggctttt
    39361 tccaattcaa acggatttca tgaaaaatct ggtctgtgtc cgtaaattct tttccctgca
    39421 aaaacaattt catgggttcc ccttcaacag atagatcact gatctgcagt cctcgatccg
    39481 caaaattcgc cccttcaaca aagtggacat tgcttaaaat cgcttcatga ttggcaagat
    39541 aattcaagat ccaaaaaact tctcttcggc taaaagatac atgattgacc atccacgtta
    39601 aaaaattctt cttttcccgt acatcaatta gcatttttct cacctctctg ttaggtcatc
    39661 taataatgac agtgcctcca tatctcctgg ttcaagtgtt aaataataag tcaataattc
    39721 ttgcgctctt ttcagctgcc cttcttctct taagaaaaag gcatactctt tcaagaaatc
    39781 tggttcatgt gatagttcat gataagcctg ttcatagtgt acagctgcaa cggtaaagtc
    39841 ttctaattca ttataagcat gggctaggtt ccattctgca taaggattag cagtttcttc
    39901 catctgattg atcacttgta tcacatcttc atatctttca tcatccaagt aaagattact
    39961 taaagtaagt aatgtttcat cttgtttgtc tcctacttct aaagctttca gtaaaagttc
    40021 ttctgctcgc tgtctatcat gcagacggaa agcattttcc gaggccaggt gataaagttc
    40081 gacttggtaa ggattttctt tgattccttc ttctaacact gtttgtgctt cttcaatcat
    40141 ttcttcttct tgaagagctt gtcctagata tagatataac gattgataat gcggattaac
    40201 ttctcgaagt tcttccagat agttgattgc ctgctgattt tctttcaatt gaagattcgt
    40261 aaaagctagc tggaacagtc gatcatctgt cctttcttct ttcagtgctt cttctaaagg
    40321 tacgatagct gcttcgaatt ctccttgcat acttaaggat tgtcctaaac gttcctgcat
    40381 cgagattcct gaaatttcat ttatcccgct tgccaacaat gtttcataaa ccactgctgc
    40441 ctctttgaaa cgatcaactg aaaaatacaa ttcagctagc gcaaactgga tcaatggttc
    40501 atcaggaagc aacccagctg cttctttcaa ttttgcttca ctcacttcag ggattccgat
    40561 aacttggtac aaatcagcta tcgccaataa gctttgcgga tagtaatcac tttgcttatc
    40621 cactttttcc aggtattcaa aagctaagtc gatctcatta tcttcgattg caatttctgc
    40681 taacgggatg ttcagtccgt cattttctgg gtattggttg atcagttgtt caaaaatctg
    40741 ttttgcttcg atcaaaaagc caatagccag caattcttcg cctagttcag ctaatatatc
    40801 tgctgggtct tcttttaatg ctttttctaa aaagatttgt gcttgtgaca agtcttcatc
    40861 tgataaagct tgcaacattt tttcgctatt tgtattcatt tcaattccct cttatctcac
    40921 tgacgatttg ttgaaatacc gtcattgttc tcaataattc tttttcgttt ggatgttggt
    40981 atccgtctat tatacctatt ttttctaaaa caagtatttt tttgcttttt tctgccaaaa
    41041 aaagaacttt ttccaaatac tccatctttg aaatctgttc cagccagtgg attggatagc
    41101 caagcaagtc cagccacaca aaaaagtttt ttagttggaa atcaaaagga tatgcttgga
    41161 gattccacac gaaatgaaac atcattccta aaaatttttt catactagca gaaagtagat
    41221 ggccattttc ggtcagataa aaagcttgtt caaacagttt accataattt gacaggtttt
    41281 ctgcctctaa ttgataaaaa tagatcattt cttcgatcat cccggcaaac ggccgagtcg
    41341 ttaattgctt tttatttgga tagtttttat acagattttg caaaaaacga tgatcacaaa
    41401 tcattccgca acagataaaa gtcaaaaagt caatctgctt tccgtccacc tgccgctcgc
    41461 tgattgtttg atcataaacg atgcgttctg gcagattgat ggtttgcagc actacacgat
    41521 ttggtagctg caaaatcgtc cgctgcggtg ttaatgctcg gcttagagaa cggatcgata
    41581 cagggatcat ccatagtgat gaatcaaggg ttgtatgttt ttgaaaaaaa cctgccagat
    41641 ccatgatccc ttcatttcca aatccgatga taaacatgga ttgattttcg ggatagcgtt
    41701 tgacgaaatc aagcaggttc tttaattctg ttaaatgatt gcattgagtg ttggcacaaa
    41761 tataccagtc attgtttgct tgatttgcaa ataacgggtt gatcttatct gcaaacaaat
    41821 cataatagcg ctgattcgtt aaaaataaga ttggctgatc aatggaaagt tctcttagct
    41881 gactgactag cgtttctccg taaaacactt tcgtcaacgc atcttctact tgataatcaa
    41941 gttccatacg catccctcct cttcttattt ttattttacc attagtaagc aaattctaga
    42001 aaagataaga gcaaagctca cctcataact tgcaaataaa taaaaattaa ataaaataca
    42061 tgtgttttct atgtatgaca tacggattcg gaatctctgt ctcttattta ttccttttct
    42121 gctaggaaat aaaaagaaaa gcacttgtag ccctagagcc acaagcgctt tttcaactgt
    42181 cttatttaac agcgtctttt aatgctttac ctggtttgaa tgcaggtact ttgctagctg
    42241 gaatttcgat ttcttcacca gtttgtgggt tacgaccttt gcgagctgcg cgttcgcgaa
    42301 cttcaaagtt accaaaaccg attaattgaa ctttttcgcc gttagctaaa gcatcttgaa
    42361 tagttgaaaa tacagcatct actgctgcag ttgcatcttt tttagttaaa tcagtagctg
    42421 aagcaacttt ttcgattaat tctgctttgt ttgccatgga tagattcacc tcctccacaa
    42481 gagatatgat agaaatatca tttagttaaa actttctgag tggtccagaa gtttaaaaag
    42541 aaatattgat taacttcaat attctgttat cacgatatca tatgaaccgc ttaatagcaa
    42601 ggtttttacc aattttcaca tgcttttttt agtcatagaa acgtaacaaa cgaaactgtc
    42661 tttttctttt gaaaaaaaaa cgtcgatatg gtaaaatgcc tattttctcc tacgagggat
    42721 aattcggatg gacgtccctt caaaagtaaa tgcttttctg atttgatttt ctaagaaacg
    42781 ttcataagaa aaatgcatca attcctcttc attgacaaaa acgacaaatg taggtggttt
    42841 gacagcgact tgtgtagcat aaaaaatctt caggcgtttt cctttatctg ttggtgttgg
    42901 attgattgct acagcatcca taatgatatc attcaacaca gccgaagaga tacgaagatt
    42961 ctggttcata cttacttgtt cgatcatttc aggcagacgt tccaaccgtt gtttagtcac
    43021 tgcagaaaca aaaacgattg gagcgtaatc aagataccgg aattcatcac gaatctcttg
    43081 ttcgaaatca cgcattgtat tggtttcttt ctttacaaga tcccatttat tcacgacgat
    43141 aataattcca cgaccagctt catgggcgta acccgctact cgcttgtctt gttcccgaat
    43201 cccttcttca gcattcagta ccatcaaaac gatatcagaa cggtcaatgg cacgcattgc
    43261 acgcatcacg ctgtattttt cagtattttc gtatactttt ccgcgtttac gcataccagc
    43321 tgtatctatc attaagaatt tctgaccact ttcagaaacg aagtgggtgt cgattgcgtc
    43381 tcgggtcgtt ccttcaatat ccgaaacaat tacacgttct tctcctaaaa tcgcattgat
    43441 caaagaggat ttaccaacat ttgggcgacc aatcaaacta aacttaatcg tgtcgtcgtc
    43501 ctcttcttca gtctcttcag agaaatactt cactgcttca tctaaaatat caccaatccc
    43561 tagtccatgg cttcctgaaa caggataagg atcgcctagc cctaaggaat aaaattcata
    43621 gatatcgttt ctcatttctg gattatctac tttattgact gctaaaatca ctggtttatt
    43681 acttctgtat aaaattttcg caaccatctc atctgcgtct gtcacgcctt cgcgtccgct
    43741 gactacacaa atgattacat ctgcttcttc aatcgcgatt tctgcttgat gcttgatctg
    43801 atccataaaa ggttcatcgc caagatcaat tccgcctgtg tcgatcacgc tgaattctcg
    43861 acctaaccat tcaccttttg cataaatacg atcacgagtt actccaggag tatcttcgac
    43921 aatcgagata cgctcaccag cgatgcggtt aaaaatcgta gacttaccga cgttcgggcg
    43981 gccaacgatt gcaattgttg gatttgccat attatttcct cctcacaagt ttccaatctc
    44041 acttagttta acgaatgcat tgccaacttt caagcataga ttgtcaatta aacgaagtat
    44101 ttttattcct tattgaaata ctaatcgctt ttcaaaagaa aaactgagac aaaagcagct
    44161 gctttcatct cagtcacaga tacaaaatca attatttttc agaatcatcg gttgcttttt
    44221 cttctacttc ttctttcaac gcatcaccta aaatgtctcc catagtaaat ccagtatttt
    44281 cttctggtaa ttcataagac tcaacatttt ttggttcttc ttctgaagct ggtttttctt
    44341 ctaaagcttt gatactcaat gcaatacgat gttcttctgg atggatctct aatactttca
    44401 ctttcacttc gtcaccttca tgaagcactt catgtggtgt cgcaatatgt ttatgtgaaa
    44461 tttgagagat atggactagc ccttctactc cagggaacac ttcaacaaag gcaccgaagc
    44521 ttgtcaaacg tttaactgtt ccgtctaata ctgtgccaac tggagctttt gtatcgattt
    44581 ctgaccaagg tccaggctgt gtgtctttga ttgacaagga aacacgttct tgttcaggat
    44641 tgatagacaa cactttgaca tttacatcat cattgacagc caatacatca cttggttttg
    44701 ctacatgact atgtgaaatc tctgagacat ggactagacc gtcaattccg cctaggtcga
    44761 caaatgcgcc aaaatcagta agacgtgcaa cacgtccact gagaacatct ccctcatgga
    44821 tagttgaaag aatttctttt ttcttttctt ctctttcgtt ttccactacc gctttatgcg
    44881 ataagatcaa acgattttct gatggttcga tttcgatgat tttaaagctc aatgttttgc
    44941 ctttatagtc tgaaaagtct gcaacaaaat gatcttccac cattgatgca gggacaaaac
    45001 cacgtacacc gacgtcaacg actaaaccgc ctttgacaac atttgtaaca ggtgcttcga
    45061 tgaccttgcc ctcttggaaa tctctttcga tatcttccca aactttttta gcatctaaac
    45121 ggcgttttga aagtagatag ctgccgtttt ctttatcttt gccgatcgta ctgataacga
    45181 ctaaatctaa tacatcaccg acttttacta cttcgctgat atcttctacc ggcaaagttg
    45241 ataattcttt tgccggaacg actccttcaa ctccagcccc ttcgattccg acaatcactt
    45301 gtttgtcttc aatgactaaa acttcgccct taacaacatc gccaactttt acttcttgta
    45361 cgcttttcat tgcgtcttcc attgactggt tgttctcttg attttgatca aattctgtca
    45421 taacacttgt cctcctaacc aacattctgc cgtaagtacg gtactgtttt tagtttaatt
    45481 gattattcaa aaaaaataaa gcgatttcgt tcgttttcct tgaaaaaaca attgtttttc
    45541 attttttttc ttaatttaaa aaagaagata accttttttc aaaacaacag cttttattgc
    45601 tgcaacgact tctggaatcg atttgccagt cgtatcgata cgaacagcat cctctgcttg
    45661 cttcagcgga gagacgtccc ttgttgaatc ataatggtct ctttcctgta tctctttcgt
    45721 cagtgtttca agatcagtcg gaattccttt ttcttggttc tctttgaaac gtctttctgc
    45781 acgctcactt acgcttgcta ctaaaaagat tttcacttct gcattcggta atacagctgt
    45841 cccgatgtct cttccgtcca tgacaacgcc accgcgttcc cctatttttc gttggacagc
    45901 aactaattct tctctgactt ttccatgtgc agagacttca gaaacagctt ttgtgacatc
    45961 tggttgacgg atttctaaag tcacatcttt tgatgctatg aatacatgct gtccatcttc
    46021 ttgttgcgaa aaagagatag gatacttttt gatcagcgaa actagccctt cttcatctga
    46081 aaaagataca ttgttcgtaa tagctaaata agttactgcg cgatacatgg caccggtatc
    46141 tgtataaata taattcaatt cttttgctaa tatcttggca acggtacttt tcccagatga
    46201 agcaggcccg tcgattgcaa tactgatttg tgtcatgata taactccttt ttctcagaaa
    46261 aaaagccgca aagcgcggct attattattt aattcttaat tcttgaccag ggtaaagcat
    46321 aaagttgttt ggatctaagc cattcaattc aaacaattga tcagtggtga tccctgcacg
    46381 ctcagccact tgttttggac cttcgcctgc ttgaacagtc gttgttgaac cggctgcagc
    46441 tgatgaagaa ggtgttgttt ctgctgcatt gctgcttgaa tcccctgctt gataagttgc
    46501 ctcacttgag gaagtcggtg ttgtctctgc tgcaatactg ctttctgacg ttacacctgt
    46561 atcttgagac acagtagatt cttccgcgat tgtactagat tctactgttg aagtctcagt
    46621 tgactcctct tttgttgtac ttgtagaact ttctactact gatgtcgaag tttcagaaga
    46681 agttgctgct gttgcattgt tgcttgatcc atttctgatc cagaaataag cacctgctgg
    46741 aatagcgata cataagacca gtaaaatcaa aaatagcgaa agaaataatg tatttccttt
    46801 cttttgttgt ctctgagaac ttcttgatga aacttcatca tctgtatcgt agattggttg
    46861 ttcccatggt tcttgtgttt cgttattttc tgtagattga tgtctatctt ttctgctcac
    46921 tgttttacct cctaaactct atcgtgtgta ttataccata ggaaaaagtg tcttgtccta
    46981 aaaaatcaat gttccatgtt ttttttaaat aaatttagta aaatcgttcc ccaatgaagt
    47041 tgttcagtct tagaaaaaag cttcctcttt ggcagaagtt ctgcttttgc tccatcaata
    47101 tcacaacaat tctgcggctt ttcggagagt ttttcgccaa aataagctaa gatgaatttt
    47161 ctgcggcagt tttcttcatt aatatagcga agcatctgct gcaggcggaa ttctttttct
    47221 ttttcctgac gtttgagccg ttccacccaa ttttctggtt tttcagactg ctggatcagc
    47281 tccatccatt ttttttgcag ctcatcaaag ggagcttgct ctgtggcata ttccaaataa
    47341 agctcaaaac tttgtcgctg ttctctagaa agttgattaa agaaataatg gatacgttca
    47401 tccccacttt gataaaaaag aattgctgca ctttcttcct gatctctgcc tgctcgaccg
    47461 atttcttgta catagttttc tagactgtct ggcaaatcat aatggaccac ataacggata
    47521 tcagatttat cgatccccat accaaatgca ttcgtagcaa tgagaaactg cagctgattt
    47581 tttacaaact gctgttgaag ttgtcttctt tgagctgcat caagaccacc gtgatagtat
    47641 ccaactgtaa aacgttccct gaataaatga tacaatcgtt cgacttcttt ttttgttgca
    47701 caataaatga ttgccgctcc ttgtgctctt tccatgaagt gttcaagttc ttgttctttt
    47761 tgctgcgttt tccgaacaaa aagcgagata ttttgccgat tgactgattg tctgatctct
    47821 tttggtggtc gatcaaatag tacatgacgt atatctcttg ctactaaatc tgttgctgtc
    47881 gccgttagtg ccagtgtgac cgggtttcct aaaagctttc ggatttttcc taactgttga
    47941 tattctggac ggaaatcaac tccccattga gacacgcaat gagcctcatc tactacatat
    48001 aacgcaatat tttgtttttt cagatgctct aaaagagaag gattagtcaa catttcggga
    48061 ctcaaaaaca aaaatttata ttgcgataga tgacggagga cgtatcttct ttcagattgc
    48121 gaaagtaaac tattgaaagc agctactcgt ttctcccctc gtttttgtaa ttgcgtcact
    48181 tgatcttcca ttagagaaag cagcggtgac acgatgatga ccagaccttc cattaaatac
    48241 cccgtcaatt gatagcaaag gctttttccc gttcctgtag gcaaaatagc gagagtatct
    48301 tctccatcaa gcaacgcctg aataatttct tcttggccgg gacgaaatga atcaaatcca
    48361 aaatattttt ttaattcatt tattaacatt ctgtatcgct tcctttctca atagatagat
    48421 ttgatacaaa cgaaattctc cataatctaa ttgtccatct acattccatt cgctaaagcg
    48481 ccactctcgc acggaatcct tatttggttc caaacgttcg attgtttctg cagagagaat
    48541 ccactcgtat ggaaaatcat caaaaaacaa ggcccactca ataagatgat ctgttactgt
    48601 tcctcttttt aaatgacgga ttgccatgat ctggtcaatt gtctgaccat tcataaacat
    48661 ctgcttggtg atcatcatgc tttgattgaa attttgctgt aataatgaat cgatcaaagc
    48721 gtgccaaagc gaatctggcc gtttttcgat ttgtgctaac aaaaggtgga tacatctgct
    48781 ttgttgcaga taaaccgctg actcatcatt gttttttgcc agctgataag gaagcagccc
    48841 tgttcgctca tttccagata attggttagc taaaaaatct gctgcttctt ctggtatctc
    48901 cgaaaacagc tgcaccaaac tctcatacgc agtatcaatc aaaatttcac gtggcaattg
    48961 gctttcacta agccattttt ttagttggaa agtataaaaa ggacttgtct ctgctggaag
    49021 atagtcttga atacccgttg ccagattaga aattacttga acagcaaatt taatgagccg
    49081 ccaactagtt tcccctgttc tgccatagcg gtcaaaacgc aaaccagtat gttctatttg
    49141 atgttcggac aacatatcag cccctgcagg cgttagcttt gcttcatttt catttatttc
    49201 gatccaacct tgctgcgcaa ttttttgcaa aatttgataa aacttatcct gcttcaattc
    49261 tggaaaactg ttatggataa acaaaagttc gtgggtaaat ccataaataa gtactgaagt
    49321 cgttcgtttt cccacaagga gttggtaaag agaactaatc cgcagcttgt gtcctgatga
    49381 aaatagcgat aaaataaaaa caatcatatt attctcctta agttaggatg gttagaaatg
    49441 tcactactat gtaaacttgt acctgaacgc tgtattgcat gcggtctttg tcaaatcgaa
    49501 gcgccagata ttttcgatta tgatgaagaa ggaatcgttt tatttgccca agaaccagag
    49561 gctcatcaac agttcgtgat gccggaagaa gaaacatatg taaaaaaagc gtatcaaaaa
    49621 tgtccagttc gagcgatttt attagaaaat aaaatataaa tataaacgaa actttaaaaa
    49681 cgtacactaa gttctctaaa cttgaaaata agccgggatc tgtttctctt gttccgtgaa
    49741 atcccgactt attttgaaag attcatttgc tatctatagt taattgacta gttttttcac
    49801 ggaccctata ggacccattt atattatttc aactttttca gccagttttc caatgtattg
    49861 acgatcacag tttgctggtc tgcgttagaa atttctgcct ctccatctcc tttttgctca
    49921 ccatagctgc caaaacctcc atgatttccg ccattgattg atacatactc tgttctttcc
    49981 ggtaaatact gcttagcttc ttgataagct tcctgattca atactttatc ttttgtcgcc
    50041 gtgatcgata aaacaggaac agctgattct gtcaatgttc cttttttgtc gggataactt
    50101 gccatgaaga ataccccttt taaatgtttg tctgaatgat tcttattgac ataacgactt
    50161 gccattacgc cacctaatga atggccaccg atcacataat cggaatttgg aaaatcttct
    50221 ttgaccttat ccgctttgtt tccgcccatc actgctagat caagtggcat tttgacaata
    50281 gcgactgaaa agccgttttt tgctaaatct tgcgcccaaa tactataact ttctggctct
    50341 acgagagcac caggataaaa aataagtaat ggagactttc catgattctt tttctctcct
    50401 tcaaaaaata agtaatcttc tttgtttttt ccttctttta ccaacacttg cgcttctgac
    50461 gatggttcgt acgtattttg ctgtatatag taataaccgg ctccaccgag taatcctaaa
    50521 atgatgacta agccaattag tattcgtcta aatattttca ctttctccca ccttttctat
    50581 ctgagtgacc cctttttctt aatcaagtat gattatacct taaaaaaata agagagaaat
    50641 gcaaactttc cagcatcaaa taaaaaaaga cggaatatcc atttgaatat ccgccctttt
    50701 tgtatgttta tttttattta gcacataaac gatacacagc ttctgcataa atttccattg
    50761 ctttatacat actacttaat tcccatcgtt cattcgcctg gtgcataaag tcaggagtat
    50821 cagggaacat cgcaccgaat gccacacact ggttcatcgt acgagcaaaa gtagcgccgc
    50881 ctgatacaaa cggttcagtc atgtctccag taatatcgcg atatgtcccc aatagatttt
    50941 tcactaactc actgtctagt ggcacgtata atgaatcaag gaagtcaaat tcttcataag
    51001 tcaacccata tttttctgct gttttagtta atttagctac taaatcatct ttcttgaatg
    51061 taacagggat tctcatatct acaccgattt ttgtttgttc tggtgtgatt tccaaacttg
    51121 caaagttcat cgttagctcg ccggattgtt catcttctgt tttgcccacg acagcttcac
    51181 cagttgcatt ttcttgaact aattttccta agaaattgat tgggccaaaa tcaaatactt
    51241 ctgaaagagc aattgccaga cgagaaatag cattcactcc ttgtgctgca tctttcgcgt
    51301 ggatactttt accaagaacg acgatgcctt caccttgttc ttcgaaatcg aattcgtgtt
    51361 ttttcaatgc ttcttttact tcagaaagct tttctccaga gtaaggagct gcatcaggaa
    51421 caacattcaa cgctcctcca gcttttacag aaaactcgtt cgttcctggt cctgtcaagt
    51481 aagcttgcaa taagcctttt tctgcataaa ttaaaggaaa ttcagcatct ggcgcaaatc
    51541 cttgcgtaat tccttcttct ttttcgttat atttttctaa acaacgccat aaattttctt
    51601 catctgtacc aaagataaaa cgaatacgtg tcttgaattc cacgccagca tcaatcaaag
    51661 ctttgacggc atacattgcc gcaatagaag gacctttgtc atcttgggat ccgcgaccaa
    51721 ctaaccagcc gtcttttata gttggatcga atggttttgt ttcccaatta ttttcgtccc
    51781 ctgctggaac aacgtccata tgacacaaga taccgaataa ttcatctcca gagccaatct
    51841 ctgaataacc gtaatagcct tctggatctt taaatgttct aaatcctagc ttttcactga
    51901 tttccaatac tttatccaaa gcttcaatca cttttttacc gaagggatgt ccttgtcctg
    51961 aatctgcttc atctagtacg gaaggaattc ggatcaattc gctcaatgat tctaatgctt
    52021 gttcatgatg ttgttctgtc acaaattttt tcatgatatt atcctcctaa tatgttaaaa
    52081 agggtcatgt caaattcctc cgacacaacc tcttcttctc aaataatgac gttttaagcc
    52141 tgctccatca gcaagcagaa tgttcttaac aaatgatgaa agaaaaaaca ctcactgaat
    52201 ggagctattg acttatccac gtgcttatcg atagccgttt atcggtttct tcattcttat
    52261 gatagacctt gtacctgtat ttgtctaggt ttagaacatt gctgctacac ctaggatcac
    52321 acagatggct acaaataata tcgcaattaa tttaccggta aatttccacc acactgaaac
    52381 atcgaaacga gcaattgcta cagcacccat tacgatagca gatgttggtg taattaagtt
    52441 tactaaacca gatgcagatt gataagcagt gattactaaa tccttaccga cgccggcaaa
    52501 ttcacccata ggtcccatga ttcccattgt tgcagcagct aagccggaag ttgaaggaat
    52561 caagaatgac atagggatat agaaaataaa tgtaataatg atgaatacac ttgaagataa
    52621 tgaacttaat ccttgttcgc cccaatgtaa gatggtgtct gtgattaatc cattgttcat
    52681 aaccacttgg ataccacgag caacagctac tacgattgct acaccgatca aatcagaagc
    52741 accagctaaa aattcagaga caaattcaga ctctttcatg ccataagtga ttgctactac
    52801 gatggacatt actaagaata acatagtaat ttctgggaag taccagtcac ctaatggaag
    52861 catgctttta cctagaaccg tacctagtac cggaaggttt gttaaccagt ttgtcaatga
    52921 ttcaaaaatc gtgaaattcg cattcaaggt tgtccaagga atcaaactga tgatcatgat
    52981 accaaaagta ataaagaaca atacattaac atgtttttgt tttttcgtga ttacttcttg
    53041 acgaccgatt gcttcaatat cgaaatgctt catatcttct ttacgttggt tgtatactaa
    53101 tgattttgtt ggatcttttt cgatctttga tgcataacga tacacatata caatagaaat
    53161 agctaacaaa ataacgaaca tcaagaatcg aagaccaata ccttcaccca tcgaaatccc
    53221 aacagcttgt gaagctacgc ctgttgcaaa aggatttacc gttgaagcta gacagcctac
    53281 ttgagaccct actaatacga ttgctacagc tactactgta tcaaagccta cagccatcat
    53341 gactgggatc agtaaagcat agaatggaat cgtttcttct gccattccgt aagtagagcc
    53401 gcctaatgca aataacagca tcaatactgg aataagcatt ttttcttttc ctttgaattt
    53461 cttcacaact gaagcaatcc cggcatctag tgcccctgtt ttgttgataa tacctaaaaa
    53521 tccaccaaca accaaaatga agaaagagat tgggattgca gcaggtgttt ctttagttag
    53581 ctcccccgct ggtacaccta gcatcccatt gattggcgcc atgaaaatat cccacagccc
    53641 ttgaggattt tgttgtactc gttcatatga tccagcgacg atgttgcctg catcgtctac
    53701 actatagtgc ccagctggta taaaccaagt aagtacagcg attactgcaa taataataaa
    53761 taagaccgta tacgctgaag gcatttgaaa ttttttcttt tttgtttgtt ccatcttctt
    53821 cttcctttct tttccgttag agacaacaat tttggctcta atgttatttt atcaaaaaga
    53881 gtatcagaac aaaaataata acacgcattt tgtaaaactt ttgttcaaaa agttcgtttt
    53941 tccctattcc ctcctctttt ttcaggtttt taaagctttc ttctttttca ttgtagcgtt
    54001 tccttttttt tataaacaaa cattttcgtc ttttaaaagg agaagaattt cacatttgca
    54061 aagaattgat tgatttttat gcataaatca aaaaaatact caaaacaaac aaataaaacg
    54121 tttacattat tgcatgtctt gtattttttt ccaaaaaaaa agaccaagaa atttcttgat
    54181 ctttttgata tttatttgac tgctgtatga cgttgttcga actgatgttg ttttcgactt
    54241 aaccatggaa gaagttgcgc atgcagcact aaaaagacag cactcacgat gaatcctttg
    54301 atcaagttga aaggaacgat cccaatcagt acatagtttg ctaaagacat gcccagcatc
    54361 tgtgttgcgt tcagccctaa aaacattaag taaagaggcg taataacgaa ataatttgcg
    54421 atactcataa aaatagttag agcgatggtt cctgtaatga cacctaaaaa tttgtttctt
    54481 cctttatgct gtttctttac actaaagaaa taataaatcg ggaatgtaaa gatcgttgtt
    54541 gataagaaac tagcagtgtc tcctaccaaa ttatccggag aaaaacctgt agtcagcaga
    54601 tgtaatactg aacgcaagaa cgctgttgca actccggccg ctggaccgaa taagaacatg
    54661 ctgatcaata ccgagatatc actaaaatcg atcttcaaaa aactaaaagc cggcatgatc
    54721 ggaaaagcaa aatactgtaa aactaatgcg atcgcagcaa acatcgctac tgcaaccatt
    54781 ttctgtacac gggtgttctt catttgaaat cctcctgtta ggactcctct tcagcgaact
    54841 ttttgacgtt tctctccaaa tgaaaaaact ctattttcca gggaaaatag agttagagtt
    54901 atttgatcaa gcgtcgtcaa ataagctcta tcttctttat ccagactata ctgtcggtat
    54961 cggaatttca ccgattcagc tatttttttg caaaataggt cgtggactat aaccaccggt
    55021 cgggaatttc accctgcccc tgaagacgaa ccttatataa tttttgttta catattcatt
    55081 atacatatct attcaaaaaa cgcaacatct aacacttata aaaagtaagt gttcattttg
    55141 agtggttcag caaactgctg acttcttttt tgttcagttc acggtaatcc ccaggacgta
    55201 ggccttgtaa agtcagatct ccgtattttt cacgttttag tttttgcaca ggaagaccaa
    55261 ctgcttgaag catatttttc acttgatgat tacgtccttc gtggatcgtc aattccacga
    55321 tactgtggtt tgtcttagga tccactgata aaatttggaa tttagctgga gatgtttttc
    55381 gtccttcgat ctttattcct tttgtcaaag gtgcaagcat cgattttgta gcgatccctt
    55441 ttacctttgc aacgtaaact ttatccactt catgttttgg atgtgtcagg cgctgagcaa
    55501 aatctccatc atttgtcagt agcaagatcc ccgaagtatc gtaatccagt cgacctacag
    55561 gatagatacg ttcttttacc atcggcaaaa gatccgtaac aacttttctt cctttgtcat
    55621 ctgcaacagc agaaataact cctttaggtt tgtacaataa atagtaaccg tattcttctt
    55681 gataaattgg gacttcatcc acttccacag tgtcatgttt cccaactttt atccctaatt
    55741 cccttacaac ttgtccgttt acttttacac gtcctgcaag gatgaattct tctgcttttc
    55801 gacgagaagt gatgccagcg tgggcaatta ctttctgcaa tctctccatt acaattattc
    55861 tcctttattc attccatcaa agaatagatc tggtgacacg tcttcttcaa gtgaaatttc
    55921 catttcttgt gtatcaggca gttcttctaa tgatttcaaa ccaaaataat ctaagaagta
    55981 gccagttgtt ccatataata tcggccgacc tggtccttct actctacctt tttcttcaat
    56041 cagtttatgg gcagctagac gctgtaccgc tcctgaaacc tgaactcctc gaatctgctc
    56101 gatttcagct cttgtgattg gctgtttata agcgatgatt gctaaggttt cgacggcggc
    56161 ttgagataat gtgtttgatg tcccttgcgc atatcgctta agtaagggag ccagctcttt
    56221 tttagtcgtt aatacataat ggctttccgt ttcaaaaata tgcaaagctg agtgcgagtt
    56281 ttgagtgtaa gattctttta attctttcac taaacttgtg acttctgctt cgctgagatt
    56341 taacagtccg cttaattctt cattactgat cccttcctcg ccaacaataa aaagaagggc
    56401 ttcaagttca cttaatttcg tcatgttcgg tatctcctac tgctggataa agcataattg
    56461 attcatagtt ttctttttgt tgcgcaatga tctgcccttt tttcatcaat tctagcaaag
    56521 ccataaaagt agtgacaact tcacttttag aaggtgactc aaaaaaagaa tcgaaagtac
    56581 agccatgcgc atttcttttt atacgctctt cgataaatac cattcgttct tcgatcgttg
    56641 tgtcatcggg cgtgatcgtt ttttccactt tttgagaaaa tttcttacgt tctaagattt
    56701 tctgcatggc acggatcaaa tcgatttttt tgacttgtcc tcgcttcaac ggcttttcag
    56761 catccgctaa cccatcgatg ttcataggtt cttttgtgta ataaagactt cgttcctctg
    56821 ctttttctgt cagtctttca gctgcatatt tatatttacg gtattctaaa agctgagtga
    56881 ccaattcttc tcgaggatct tcttcccata attcttcatc gtctgtgacg aattcttgga
    56941 caggaagcaa tgtcttgctt ttgattgcca tcaatgtagc agccatcact aagtattctc
    57001 ctgcaacagc tagctcgagt gttttcattg tatggatata attcatatat tgctccgtga
    57061 tcgtagcgat cggtatatca tatatatcga tttccatttg ctgaatcaga tgaagcagca
    57121 gatcgagagg accctcgaat acatctaatt taatattgat ctccgtcaac tcattcactc
    57181 tttcttcttt ttctttgcta gccattttcc aatgtaaggc tctgtgattt cagttgcacg
    57241 gatggagaca tccgggtact tttcctgtaa ttcatctaac atttgaaagc ccatattctc
    57301 accgcgataa gacggattca aagaaatgtc atggatgatc agctgatcgt tcgttgccca
    57361 gtaaatacca ataatccctt gaatgttggt tgaaccttct ggcgtaaaga aaaaaagttc
    57421 ataatcgtct tcagattcgt acgtatcaat ctccttcaac aaagaagttt tacctgtcag
    57481 tttcttatgg aaactcaata gtcccatagc aattttacgg ttttctttcc ggtaatgtgt
    57541 taacatcttc tcaccttcct atgactaagc ccttggaaaa tgctgcttat aaacatccgt
    57601 cattcttttc ttcgtgatat gtgtataaat ctgagtcgta gagatatccg cgtgacctaa
    57661 caattcctgt actgtccgca aatcagctcc attttctagc agatgtgtgg caaagctatg
    57721 ccgcagcgta tgtggtgtta cttctttgta aatcccagcc tctcgtacta actgcttcag
    57781 atttttccag attccttgcc gagaaagacc tttcccatga ttattgacaa atacatgcat
    57841 ttcagatgcg tcctttacaa gtaatggacg tgcttcgtct aagtagcgtt ccagccattg
    57901 gatcgcataa tcacctaaag gaatgatccg ctctttatct ccttttccaa ctgtctgtac
    57961 cagtccaaga gaaagatgaa gatctcccaa tttcaatcca atcaattcac tgacgcgcat
    58021 cccagtagca tacatgactt ccaagattgc acgatctctt atccctaacg tttttgtcgt
    58081 atcaggtgct tcaatcagct tttctacttc tgtcagagtc aatgtacttg gtaatttctg
    58141 tgctttcttc ggcgtatcga tatgttgcat cggatcatga tcagtgatac gctcttgccg
    58201 taaaaactga tggaatcttc tgagacttga gatcatccga gtaacagttg ctgtcgcttt
    58261 tttttcttca tgcaacttct ccagaaaacg gatgactaaa aagcgatcga catcctgcca
    58321 tgaagcaatt ttctgctcct gtagaaaatg caagtactgt tccaaatcac gttcatagct
    58381 ttgtcgtgta ttggcggaca atccccgttc aatattcaag tagtggagat aatcgttgat
    58441 ttgctctttc ataataatcc ccctcatgcc tctctatcat atcagatacc gcttcattct
    58501 gatagtcttt ttccataatc tgagcaaaga aaaagccgtc tagacactca gacggactgg
    58561 tttctttttt ctttttcata gcattcctga cagattccat ggaacgttag ccgatgatct
    58621 tttactaaaa aatgataacg ttcttctact acttgctcca cttcaagtaa aagatcctct
    58681 tctacttcct cgatcgtgcc gcagttcaag catagtaaat gatgatggaa atgtttggcg
    58741 ccttcttttc tcaaatcata acgggctaat ccatcattaa aacttacttt atcgacaatt
    58801 ttcaattctg tcaagatttc aagggttcga taaacagtag caagtccaat ttccggactc
    58861 ttttttttca ctaaaaagaa gatttcttct gcagataaat gctctttctc attttccaaa
    58921 agcacaagca gagtagcttc tctttgcggt gtcagcttaa accccgcttc atgtagctgc
    58981 tgttttgttt ttttcatcgc cgaaatagca ttcatgacca tttactcaac cccaacaatt
    59041 tataataatt acaaataaaa aaagatataa cactatgtta attaaaatta ttataagcta
    59101 gttaagaaaa acatgcaagg gattctcaat tagatttttt gactagtttt gtcacaccgt
    59161 tttcttgtgt aatcaatcct tgtttcaaaa gattacctaa tgcgcgctta aatgctcctt
    59221 tgctgatacc aaaagctttc atgatttctt ctggatccga tttatctgta aagttgattt
    59281 gctgatctgc agaacgttct aaaatggtta aaatcatttg tgcatcatct gatatagctt
    59341 catatgctct tggcttcaat gaaagattca gtacaccgtc tggtcggaca ccaatcactc
    59401 gaccttttat ctgctcgcct agacgaggtt cattatatcg ttcagaagga tggataaacc
    59461 caagatagaa atcttccgtt aaaacgtacg tacctgttaa tttcaagcgg tatgctgttc
    59521 ctatcacatc tttgtttttc atttcttctg ttccatattt gcttaaagat ttgaatattt
    59581 tttcatctgc taaactgccc caaatacggt cttttgcatc aactctaagg gaaatcatca
    59641 agcgatcccc ttttttaggc catagctctg tcattgtcgg caattcatca agagatacga
    59701 caaagtcttt atctggaaga ccgatatcga caaacgcacc taaatctttt cgtgtagcag
    59761 taacgattcc aaaagcgtaa tgaccgatac ggcttttagg aatttgtgta gtgatccgat
    59821 tttcatgttt ttgattttgg taaccgaatc cttcaactgc ttcaccgatt ttgtgttcac
    59881 cttctgattt gcttaagcca aaggtttgtc cgtttttttg gacaaagtag tttttttcat
    59941 tttcatcaat aataatacct gtaaatatag ttgctaataa atcgttcatt tttttctccc
    60001 tctatagtga aaggttttct taatacattt ctttgctatc ataacataat gttatctact
    60061 tgaatagctg agccttggtt tggaaaggtg aaaaaaggag tatgacaaca gttgtgtcat
    60121 actccccttt ttcgaataaa cggtgttaaa cggcaggctc acagcctctt tcatttgcct
    60181 aaaattaatt gctcccattt cctccgaacc atccagtttt acagttgatg gttacgatgt
    60241 aaaatggcaa agcactttct ccaatgcctt tcatcaatgc agattttgtt gaagtggcat
    60301 atagattata atagccttcc ccattttcga ttctggcttt ttctaatctg taacctcctt
    60361 caacgatata tccacgttgg atacatgtag caatcacttt gctttggaca cctggtgccc
    60421 acgtccatgc tggattagat gcatcggcaa tatcacttgc tctggcagat ttgattggct
    60481 gattcgctga gtttgaagag gaaggggtcg aggatgccgc ttcttgctgt gctgcttgtg
    60541 cgaccgcagc ttgttgagcg gcttgagctg cagctgctgc ttgagcggct tgttttgctt
    60601 gttcttcagc tgcttttttc gcttcttctg cttttttctt ttcctcttct tcttttttct
    60661 ctttatcagt gagcatctta tctacttgtt tcaattgttc attcaactga gcagatagat
    60721 ctgtattttt cactttttct actgcttctt ttgccgtctt gtattggtcg cgtgttgctg
    60781 aatccactac ttttccgtct ttaaagacta tagctgtttt ttctttagct gtttgcaatt
    60841 cagcatattg atttttagct tctgtttgtg cttgttgaat caattttcca aaatctgtgt
    60901 tatccgtttc gattgcttgg atctctttat ctgctttcaa cttgatgtca tctgctagat
    60961 gatcatcgac gatggcaggt gctatgaacc actcattcac tttttggata tctcggatct
    61021 tagattggat atcttggtaa gttgtttcta actctgaata tcctttttca ttcttaagac
    61081 tggataaatc attttttagt tttgataaat cttgatgaat catatctgca cgaataaatt
    61141 gatggtgatc atctgtataa aaggcagcaa gtttctcatg aagttcttcc atttgttgct
    61201 gtctttttgc ttctgctgct acttcggctt ctactttttg atgatggtca taataagccc
    61261 agcttcctcc accgagtaga agtactgctg ctaaaacgag ataaggtact ttttttgatt
    61321 ttttcttttg cgctttatct ggatctgttt tttctaagtt tgattcttga tgtgtttttt
    61381 cagtatctgg cacgattgtt attgatttcg cttctgcttc aaacagctta ggctggttct
    61441 cttctttttc tatttttgct tcaagatcag taccctcttt gcttacagat tgattttcag
    61501 catcggattc actatttatt tcatcgggct ctgttgactt tttgtctgtg ttttcgagta
    61561 tttctttttt ctctgcttcc ggcagtttat tcgttttttc cttttctaat tgtttttcag
    61621 taaatacttc agttttttct gtgatatccg tttttacgtc ttttattttt actgtttctt
    61681 tacctgtggc gttttctcta tcagagtctt tttcagtttc ttgttccttt tctggctcta
    61741 ctttgttttc ctgtgcaatt ttttcactga tcattttgag cagctctttc gcgtgctctt
    61801 cttttgtatc ttcacgatgt gctttgatat aagcggatag tatcgaattt tcttcctcat
    61861 tatcgacgat ttctttcagt tcagctactc ttcttttttg ttttgattca agcgtttctt
    61921 cttgattctt tcctgattct tccgaagtct catgaacagt tgctttcttc tgttcatttt
    61981 tctctatatt agttacttga tcgtgagcag gtggttcttc gtcttctggt tctgcttttt
    62041 cgaataattc cattacagat tctagaggta aatctttcaa ttccgaccat tcgattggat
    62101 cgtttaattc tgtatcagga aagactgcat cttcagttga tttatcctgc atagctgcat
    62161 ctgtatttgt ttcttcaatt gattctttta tttgctgatc ttctgcagtt atttgatgac
    62221 cgcagtctgg acagatagta ttctgctcca tttttttcga tccgcaattt ggacattttt
    62281 tcatcactta gcgatcggct cctttactag gtttacgcta ccatttaatc tatcatattc
    62341 tatacgtcac gccaatagct tatttttagg atttacttaa aaataatgaa tgaatttcct
    62401 tcctatagaa aaaaaagctt gagactgcgt agtctcaagc tttttttctt ttaaaataaa
    62461 ccaaacagtt ttactgattt ctcttctggt caatagtcca cgcttattct aagaaatctt
    62521 ttaattgttt tgaacgagaa ggatggcgca attttcttaa tgctttagcc tcgatctgac
    62581 gaatacgttc ccgagtaaca ccaaatactt tccctacttc ttccaatgtt ctcgtccggc
    62641 catcatctaa accaaaacgt aagcgaagga cattctcttc ccgatcagtc aatgtatcta
    62701 gtacatcttc caattgttct tttagtaact cataagctgc gtgttctgct ggacttgttg
    62761 cttcctggtc ttctatgaaa tcacctaagt gagagtcatc ttcttcaccg attggcgttt
    62821 caagggaaac aggttcttgc gcgattttca agatttctcg gactttttct gttggaagat
    62881 ccatttccgc accgatttct tctggcgtag gttctcttcc aaggtcttgc agcaattgac
    62941 gttggatacg tatcaattta ttaattgttt caaccatatg aactggaata cggatcgtac
    63001 gtgcttggtc agcgattgct cgagtgatgg cttgacgaat ccaccatgta gcataggttg
    63061 aaaacttaaa tcctttgcgg tagtcaaatt tttcaactgc cttcatcagt cccatgtttc
    63121 cttcttggat caagtcaagg aactgcatgc ctcgtcctac ataacgttta gcgatactta
    63181 ctactaaacg aaggtttgct tctgccagac gctgtttcgc ttcttggtcg ccttcttcga
    63241 ttttcaatgc taacgctact tcttcttccg cagttaaaag ggagacacgg ccaatttctt
    63301 tcagatacat acgaaccggg tcattgattt ttacaccggt tggcgcagaa agatcttctg
    63361 tttttgcttg ttcagctttt ttctcagcac tcttcaagct gtgaattgat ggatcaccat
    63421 tttcatccac cacactgata cctgcgtctt caatcttttg gatcaatttg tccatttcat
    63481 ctgcattcaa agtgaatggt gtagcaagct gattggataa atcatcatat acaacttgtc
    63541 gtttgggttt gttttctttg atgaaagctg ctactgctgc ttcatacgtt tttttatctg
    63601 ttccatttgc cataaaagaa ggcccccctt gagtagctat ttggcttgtt ttaactgttt
    63661 cgttaaattg atgatttcaa tcgtcaattc aagctcaagc tgtttgtttc cagttcgtct
    63721 ggcttcttgc tgcatttgtt tttttaatgt tatttcatca gctaagccag atttgctgat
    63781 cacatctagc aagtcttgga tttctcgtgg cgaactttct tctgccaccg gtaaatattc
    63841 gatttccacc accaaccgcc ttaaattttc atcttgcaaa aagtctaaaa actctgcaag
    63901 gttgaattca taatgttgtg agacatatgc atcgaataaa aaataaactt ctgcatatgc
    63961 atcatgaata aactgaaatt ctcgttcttt taaaatatta cggacagaaa cttctttaaa
    64021 tgcacgatat aatagaatct gctctgcctt ttctacttgc gtcaaaggtc tctttttccg
    64081 tgcagtttct tgtctcgtaa cctgagtgtg ttgactttcc tgaggttctt gacgattttg
    64141 ctgatttgtc cttcgaagat cgcgcagttg tttttgtatc gtctctctgc cgatctgaaa
    64201 ctctgttgac agttgagata gatacatatc ttgttcgatt ggtgaaacaa ctcggctaag
    64261 ttcaactagt aattcttcga ggtattcgat cttgtctttt tcattatcta aatttttccc
    64321 ttgttttaaa taacgtgatt taaaagtaaa gactgtttcg cgcccatgag ccactaattc
    64381 ttggaaagat gcttctccat acttacggag atattcatca ggatcaagct tttctggaac
    64441 gactacgaca gaaagtgcta atcgactgtt ttgccgaagc aattcaatcc ctcgattggt
    64501 tgcttcaaaa cctgcgttgt ctccgtcata gcaaaagacc aattctttcg tcagcttttc
    64561 catttgttgg atctgttgag ctgttagact tgtccccata gaagctaatc cagttttgat
    64621 accagcctgc catgcagcaa tcacatccat aaacccttcg aacaacaaaa tttctccgtt
    64681 tttccggata tcgcttcttg ctttatcaag attaaataaa acttgtcttt tattgaacag
    64741 ttctgtttct ggactattca agtatttggg ctgatctttt tcctgccctt tttcaggtgc
    64801 aagccagcgt cctgaaaaac cgatcgtctg gccttgagaa ttacgaagtg gaaacatcac
    64861 tctttgataa aaacgatcag acatacggcc atcatcatgc gtgacaaaca atccagagcg
    64921 ttctaacaat tcatttgaaa ttgattcatt cttgaatact tgctgaagaa aagaacgctc
    64981 attaggtgca aaacctatgt tgaattcttc aattaattct aaagacaacc cacgactttg
    65041 caaatattct aaagctggct gtccagcttt tgtatgcaat agcatgtgat gatagatttc
    65101 cgctgctttt tcatgagcag caatcagttt ccccgtagca gattcaggat tttggacagc
    65161 tatagtacgc tgacgccatt gatcttctaa cggtatctgt tcgaagtctg ccacttttac
    65221 cactgcttct ggaaaactta gaccttctag ttcttgaaga aaagtaaaca cgtttccgcc
    65281 tttaccacaa ccaaagcaat gaaaaatctg cttgtcttct gcaacagaaa aagacggtgt
    65341 acgttcttca tgaaaaggac acagccccaa gtaattttta ccagactttt tcaactggac
    65401 atattgtcca atcacctcaa cgatattcgt ttgactgcgt actgtttcga taacctcttg
    65461 aggaatccgt tgagccattt tttcaccccc tgctctattg atatcttttg ggtttaacag
    65521 aagatatttc aggagaataa ccggtaaaaa atcgcactaa ctgaaatcag tgcgatttta
    65581 aaggacacaa ttatacatgt ttgattgtat catatttcat tgctttttca agtataacac
    65641 atttctatct cattaagcat caatttgcta ctcttttcag taactgattt tgaatatcag
    65701 ccttccagta caatactcga agaacaatat ttacgagcat gaatacaaag aagtatccta
    65761 cgctctctcc caaccctaag tttacttcca aaattccatg gaacagcatt gcagctacat
    65821 atatccctac gatcgtcgtt actgctagga agatgcgcaa tgggttgaag ggataacaag
    65881 cttttacaac tgccatgcaa ctgatcccaa tcagcaagta atacatcaaa gtagtagttt
    65941 cagcatttcc ccatgcgaat gtttttccaa gtagatacac cgcaatgata ttcactacta
    66001 caagcaaggc atttggtaaa gcattaataa gtgctgtagg caaataacgg taagtgattt
    66061 ttcttttatc ttgttcaaaa gataaaaaga aggaaggata cccttcaatc gctaaatcaa
    66121 tcagcgtgat ttggataggg ataaacggaa atccaattgc tgtaacggca catataatcg
    66181 ataaaataaa tgaataaatc gttttgataa aaaagatgcc tgagactttc gtcacattgt
    66241 tgaccactcg gcgtccttca aacaggactt ctggcaatgt agtaaaatca gagtccaaca
    66301 ataccagatt cgcaatctga cgtgttgctc catcaccttc tgccatagca atgctgcaat
    66361 ctgcttctct taacgccaaa acatcattca caccgtcgcc tgtcattgca accgttctgc
    66421 cgctttcttt caattcattt accagcaatt tcttttgctg cggactcact cgtccaaaaa
    66481 ccgtatagcg atgagcagct tctcgcactt ccatttcttc agtaatttga gaaagatcga
    66541 catacgcatc ataattcggt aatcctgcac gtctagcgat attggagaca gtaaaaggat
    66601 tgtctccaga aatcactttc agatccacac cttcctcatg aagataagct agtgtttctt
    66661 gagcgttttt tcggattgga tcatcgattt caaacagagc aaccggctca acaaatgctg
    66721 gtttttcttc gttcaattgc tgatttggag cgatccccaa cataagtacg cgatagccat
    66781 tcgcttgggc tgtttttaga ctgtccggaa gccgatcttc ttccaccaaa cgttcaggcg
    66841 ctcctacgta cacgactccc agttctggaa attccatcgc gccccatttg cgatcagaag
    66901 aaaatgcgac tttgtttgtc acatcatacc gattagaaag ctcaaaataa tctctcatag
    66961 cccgcatagt gatgttgtta tcactgcttt ctgccagata cgtccccata atttctggga
    67021 tggctttctc gtacagcgga tgtagtgctt ctacccgctg gatactcatt ttcccttcag
    67081 taatggtccc tgttttatcc aagcataatg tatcgacatg ggccagcgtt tcaattgaat
    67141 acatgtcttg aacaaggatt cgtttttttg ccagtttggt tacccctgtc gttaatgcaa
    67201 tacttattag caataccaaa ccctttggaa gcatgcctaa caaagctgct gcagatgcaa
    67261 taacagaaat tttgatagaa gcatcgcgga tgaacagtgc ttccaaaaat agtatgatcc
    67321 ccaaaggaat aatcacgaaa ctagtgaatt tggatacttt gcgaatcgaa tctacaagtt
    67381 cagaacgaat agctttgtga gtctttgcct cgctagtcaa gcggacagca taattatctg
    67441 cacctactcg gataacttca gccatgacag aaccgcttgt caaaaagctt ccagaaagca
    67501 attcagctcc agtttctttg acaatcaaat cagattcgcc tgtcaaaaga gcttcatttg
    67561 cttctgcccg tccagatata acctgtaaat cggctggtat ctgttctcct gctccgattt
    67621 ttaccaaatc tcctaatacc agctctttag tagaaatcga ttgttcttta ccgtcccgga
    67681 tcactagtac ttctgattgg gaaacaatcg ataatttccg tacaagattt cttgcatgta
    67741 tttcttgata aattccaata acgatattga cgacaataat tgctaaatac gccatgttgg
    67801 aatacgcacc tacaaataac agacaaatcc caataccaaa gttcagaaaa ttgaataacg
    67861 tcaatatatt atcgctgaat atttgttttg tactttttga tgcatcttcc tggtagtcat
    67921 tttgctggcc agaagccatt ttctgaacta cctcttgctg tgtaagtcca ttcattttct
    67981 ttttctccat tttccgccaa ctttcaaaaa taatcacgtg tttaagtcta gcgtgttcca
    68041 agattaaaaa caacttgttt ttataaaatt tatagaaaaa cacacgaatg attttacaaa
    68101 aaagatgcca acctttttgt ttgaatcacc cgtgtgctgt ttttattatc aatctatctt
    68161 tacttatatt gttcctccaa acggctcatc caccaaccta gtgctgtacc ggctataaaa
    68221 agaacaggac aagctagtac cccagcaact cccaatcccg cgtaatcggt aggaatgatc
    68281 ataatcgttg aagcaaaaac tacacctaaa atgaaatgga atagcttgct atatgccttt
    68341 gagaaaatgt aatccatgat ttttgacaat ccaagaacac aaagaatccc accaattccg
    68401 atcggcacaa tcacttcaag tcgtacttct ttaaatccgt cagccatcgc tttatacaat
    68461 cccatataca ccaaaaaatt agaagggctc aatcctggga cgatcatacc caaaccaatc
    68521 aatccgccgg caatcagcca tgttccaaaa ttctgatcga cttgcccgtc aaataattga
    68581 gcgccgtata ataaaaacaa ataagccaga acaaaagtaa caaccatgat gatcacatca
    68641 gaagtatctc ttcctttttt ccctgcttct ttccataacg caggaacagt tccagcgata
    68701 catccgacaa aaaaccataa tatcgttgtt tcatacgcac ctaataaaaa actgactaaa
    68761 aaagaaagta gaaagatgcc tgtcactccg cctagaccaa caggcagaaa aaacaaaaca
    68821 ttttctttga aattttttgt aaggtgggct aaaaaagaaa tcagccgctc atacatgcca
    68881 aagacagctg ctaatgcacc accgctgact cctggcaaga taaatcctga accgataaac
    68941 attcccttca caaaccgtaa catccagtct ttggttgtgt accccacttc ttgattttcc
    69001 attttttgcc ttttttccac tatttgcgta ctccttcttt catttaattt tattcatgat
    69061 tgaacatttc ctgctatttt acttaaagac tttttcatag gtctctatta ttttttcatt
    69121 cattttgaca atatcaggat aaaacatgtt acctccatta tgatagagat agccgccatt
    69181 gtaaagcaag gactggatcc gccaatatcg gtattgtttc ccttcctcat ttcctaaaag
    69241 tgggctcagc acatctttag aatacgcttc tgctaattct actgtgtttt ctccgccatg
    69301 attttttaca taggcaatat aatctttccc aaaattatat gcttggacag ctgtccataa
    69361 atcgcatcct tgtttctttg cttctactat catttccgcc aaatatttga caccttgatc
    69421 aatactgtca gctggattat caatgatctc agcttctcca tgcagacttt ccgagctctg
    69481 catgagatct gtcccttttc cttttgattc ggtcaatatt atcgccttga tcaaattctc
    69541 gtagttttcc atatgataat tttttgcagc tgttgttatt tctatttgat agctttccac
    69601 tcgttttacc gaacgataag ttcttcctca taaaaacaac accgcaccaa taaccgtcag
    69661 aatgatcatg taaaaaagaa tacggataat agataatcgt tttcgcttca tttcatcacc
    69721 tagttatttt gacatctttt tttatcttac tatttcttta tcttcttggc tatttcttcc
    69781 ttgatttctg tgtacgcctt aagaaaaaat gaattttcct gtattttttg acatcttacg
    69841 aaaatgatcg ttgtttttca gtaaaaacaa ttgcccaatg actgatataa tcgttatact
    69901 ttacacagta ggtgattatg tgaaaaaaga aaagaaatat gcaaaaatgc cagatgggag
    69961 cgaaatttac tatgaaaaga gcggtcaagg cttccctctc tttttactgc atggcaatga
    70021 cggcagcggc cgcttttttt cagaacaggt ccctgtactc gaacgctact ataccgtgta
    70081 tctagtagat agccgaggac atggtcgttc gacaaacgaa gcatctatgt tgaattttca
    70141 gttaatggcc gaggatctga atacgatcat gttgctggaa aaaatcgatc aggcagattt
    70201 tcttggattt agtgatggag caaatctagc actggtattt gccagcagct tccctaaaaa
    70261 agttcatcga ttgattttaa attctggaaa cacgcttgtt aaaggcgttc gtttttctgc
    70321 cagagtcatc agtaatattc attatgcatg ggtatggtta ctctctttat ttcgcccaag
    70381 tcttcgcaaa aatcttttag ttatcaaact tttgctgcat gacatcgggc ttacagaaaa
    70441 tgatttaaaa aaaatcaact cccccacttt gatcatcgtg ggaaaaaaag atgtaatcaa
    70501 gctaaaacat tctttgtata tagctaaaac tataccaaaa gcttcctttg tgcttgtgaa
    70561 agaacaagga catgaattag ctagaaaaga tcctgaacgc ttcaatcgag aagttcttca
    70621 atttctttct gaaacataac tattaggaat agaaggtgat ttatttgtta aaaaatacca
    70681 tacaatggct gaaaaaccac ctaggactgt tcaaaacaat ctttttgatt tctgtcattg
    70741 tcattattgt cggtgaattg atgtccatcg gtaaaactct atctatccag caattaggag
    70801 aaacgtttgc cactatacct gtatggaaaa gcggactaat gctattgatt ggtcttatct
    70861 ctgtgttacc tatggttggt tatgatatta ttttgaatcg gatgcttgaa caaaagcaga
    70921 atcctcgtta cctgtttgag acaagctggc tgatcaatac gatcaacaat attgccggtt
    70981 tcggcgggtt tgttagtgtg gggcttcgct ctgaactata tggacaaaag aaagagagca
    71041 gaaacgtgat ccaagcatta tcgaaagttt tcttgttttt gatggctgga ctttctattt
    71101 acagtctgct ttctttttta cttgtagtct tcacgccagt tgctcctttt ttgaagcaat
    71161 attggatatg gttgatcggc ggcggtctat attttcctgt cgtcttttta tttactactt
    71221 tcaaaaagaa aggattcctt ggtggtacct cacttaaaac tcaaggacag ctattgctag
    71281 tttcattgtt ggaatggacc ggtgtcctgt ttagtttcct ttctgttggt tatttaatgg
    71341 aaatccggat tgatctgtgg caaacaattc cattattcat tgctgcttca gtgataggaa
    71401 tcgtgtctat gattccagga gaaattggga gtttcgatgt aatgatgatt atcgggttgt
    71461 ccgctattgg tgtgccaaga gaaatagtcg ttgtgtggat cttgctctat cgcctgttct
    71521 actacatcat tccgtttttg attggaatcg tctttttctt caaaaatatc ggttccactt
    71581 ttgatcagcg ttattctggt atccctaaac aattagcaac agaaattgcg cataaaatcg
    71641 tggttgtctt attatacttt tctggaatca tgctagtact ttctgccact atcccgcaag
    71701 cttttacaga gtttcgctgg cttcatagtt tgaacccatt gaaatttcat tttattatcc
    71761 aatttcctag tatactattg ggatttcttt taatcattat gggcagaggg atcgcagcac
    71821 gtgtgaaacg agcatatttg cctacaatct tcttgattgt tctagcttta ctttatgttt
    71881 tattaagtga ttttagtttc acaccggtgg ttttcttgtc tattctgctt ctgatcattc
    71941 ttgcttctaa aaacgaatta tttcgggaac aattgatcta ttcatgggag tggcgtacga
    72001 tcgatggaat cattattggt gcactgtcct tgctttacat cgttattggg gtatacaatc
    72061 tgccggattt ccctcatcgg cgtcaccatt ttatttcctt tttcttattt ccttctgaaa
    72121 agatttggtt ttcgggattg ttggccatca tcgctgttag tttcatgatc gttctatttg
    72181 ttcatttcct tcaaggagaa aagaaacaga ttggcgaagc ctttaatgaa gaaaaggccc
    72241 tgaagatcct tacaacttat ggaggaaact ccgatagtca attgatcttc ttaaaagata
    72301 agcggatgtt tgcctatgaa aaagacggtg aaccaactgt tcttctccaa ttcgcttgct
    72361 tcaataataa atgtattgta atgggtgatc cttctggtaa gaaagaagat tttccagaag
    72421 caatcgaagc ttttatcgaa gaaacagatc gactatgcta tttaccagtt ttctacgaaa
    72481 caagcgaaga gattgttatg attttgcatg aattcggcta cgatttcatt aagatggggg
    72541 aagaagctta tgtagacctg aatagtttta caacttcagg gaaaaaaatg aagggcactc
    72601 gtgctgtctt gaatcggatc gaacgagaag gatttacatt tgatgtctta caaccaccat
    72661 tttctactga gcaaatgagt actttcaaaa atatctctga taattggttg ggttcacgaa
    72721 aggaaaaagg gttttcttta ggctttttct ctgaagatta tctacaaaga gcgcctattg
    72781 cagttgtcaa aaatacccaa gaagaaattg tcgcttttgc aacgatcatg cctacttaca
    72841 cgaacaataa agtaggaacg attgatctga tgcgatatga ttctaaaaaa gcaccttctg
    72901 gcagtatgga tttcttgttc ttgcatttgt ttgcctatat gaaagaagaa catatccaat
    72961 ggttcaattt agggatggcc cctctttcaa atgtcggaac ttccagaaag agttttttac
    73021 aggaaaggat tgcatacttg gtttacgagt ttggttctca tttttattct ttccatggat
    73081 taagagaata taaaagcaag tacgctacaa actgggtctc acgttatact ctatattcta
    73141 gagatagttg gattgcatat gttatgattg ctttgctgat tattgataat gcgccagttg
    73201 agcagacaca aggtagaata agtggtttga aaaaatggat acgaagaaag tattaaaaac
    73261 tgcataccga tagatgaaca ctctcatgat gaataaaaga gactgaagta aaacgaaagt
    73321 tcatgcttct acaatctctt cttatacaat tgaagaacaa agagcggtcc aaacagaaat
    73381 cctgtttgga ccgctctttt attttaaaca tttttcatta ccaaaatatt cgagtcgcgt
    73441 ttcttatgct tcgatctctg taacgatacc tgaacctact gtccgtccgc cttcacgaat
    73501 tgagaatgtc gttccgtttt caacagcgat tggatggatc aagtcaacat caatcgtcac
    73561 gttgtcacca ggcatgacca tttctgtatc ttctggcaat gtgattgttc ctgtaacatc
    73621 agttgtgcgg aagtagaact gtggacgata gttgttgaag aatggagtat gacgtccacc
    73681 ttcttctttt gtcaacacgt acacttctgc tttgaatttt gtatgtggag taatggaacc
    73741 tggtttagca agtacttgtc cacgttcgat atcgtcacgt tggatccccc gtaacaacac
    73801 gcctacgtta tccccagctt ccccgtaatc taacgtttta cggaacattt ctacacctgt
    73861 tacaactgct ttttgtgttt caggtttgat ccctacgatc tctacctcat caccgacacg
    73921 aacagcacca cgatcaatac gacctgaggc aacagttcct cgacctgtaa ttgagaagac
    73981 gtcttccact ggcaatagta atggtttatc tgtatcacgt tctggtgttg ggatatattc
    74041 atctactgta tccatcaatt ccataatagc agcttcagca tctggatcgc cttgcaatgc
    74101 ttttaatgcg gaacctttga tcacaggagt gtcatcgcct gggaaaccat attcgctcaa
    74161 taactcgcga acttccattt ctaccaaatc gatcaattct tcatcatcga ctaaatcaac
    74221 tttgttcaag aaaacaatca ggtattttac accaacttgg cgtgacaata aaatgtgttc
    74281 acgtgtttga ggcatcggac cgtcagttgc agaaacaact aagattgcac catccatttg
    74341 tgctgcccct gtaatcatgt ttttcacata gtccgcgtgt cctggtgcat caatatgcgc
    74401 gtagtggcgt ttttctgttt catattctac gtgtgcggta ttgatcgtaa tcccacgttc
    74461 acgttcttct ggagctgcat caatactagc atagtcttga ggatttgcta ggcctttttt
    74521 gcctaatact gtagtgatcg cagcagttaa agtagttttc ccatggtcaa cgtgtccaat
    74581 cgtaccaatg ttaacgtgtg gtttacttct gtcataatgt tcttttgcca taatttacaa
    74641 acctcctaaa taatatagat cactgaaaaa tttcagctct caccttttta ttatagtctt
    74701 tttttgacta aatcaaaaag aatacgtccg acttactttt tgtaagattc gaactaagct
    74761 tattatacgt caatattggt caaagtcaac taagagcact cgtctgtctt atacgcgaaa
    74821 aaagtacctt ttataaataa aaaaatgtgt tttttgagaa aaacttcaag atagtcctta
    74881 actttctttt gaagcaataa gaacaaaatg aggaaaagtt taaaattcag ctgttgccaa
    74941 caactttcaa tagacgaaat cttctatttc gttaataata aggaaagagg tgaagtacat
    75001 gaaaaaatta attggcgtac ttgtcgtttt aggcatattt ctcggcggaa gttttgcagc
    75061 ttatcattat ttttacggcg gcgaagctta ttatacaaaa atcacgacga atggcgaaat
    75121 gtcagctacc caagcagata acggagagaa attcacgact tatacctaca agcaagcggc
    75181 ttataataaa gatggtgaaa aaaaacaggt gacgcttcgc gaagaaagag aacatccatt
    75241 aaagatgaat gcgtatctta aactcaaagt caatcctcgt aaaggtgtat taagctggga
    75301 agaaataaag gcttctgaag tgcctaaaaa tgcggcagaa aaaatcgata attagtaaat
    75361 ggtcttaaaa ttttattaga taaaa
//
LOCUS       DADVXU010000004        64463 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.004, whole genome shotgun sequence.
ACCESSION   DADVXU010000004 DADVXU010000000
VERSION     DADVXU010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 64463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.004"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..616
                     /locus_tag="KB953_000244"
     CDS             <1..616
                     /locus_tag="KB953_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HBB6865250.1"
                     /translation="FVFAPLEGEDLASFLVGAAIMMIGMTLFLFGADYSMMEVGDLVG
                     KYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQQVYDISDGEIGRVLLIGIVSVGT
                     GVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFTSSEFMPIAFDSGGVTTGPVTVPF
                     ILALAGGMTSMIKQKKNENDSFGMVGIASLGPILAVMILGVIFQ"
     gene            613..1386
                     /locus_tag="KB953_000245"
     CDS             613..1386
                     /locus_tag="KB953_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HBB6865251.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            1383..1781
                     /locus_tag="KB953_000246"
     CDS             1383..1781
                     /locus_tag="KB953_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HBB6865252.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            1966..2637
                     /locus_tag="KB953_000247"
     CDS             1966..2637
                     /locus_tag="KB953_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865253.1"
                     /translation="MNFLVVLKDTLIERFTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            2696..3136
                     /locus_tag="KB953_000248"
     CDS             2696..3136
                     /locus_tag="KB953_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865254.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            3148..3816
                     /locus_tag="KB953_000249"
     CDS             3148..3816
                     /locus_tag="KB953_000249"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBB6865255.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            3880..4503
                     /locus_tag="KB953_000250"
     CDS             3880..4503
                     /locus_tag="KB953_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HBB6865256.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            4559..4945
                     /locus_tag="KB953_000251"
     CDS             4559..4945
                     /locus_tag="KB953_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HBB6865257.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            5158..6549
                     /locus_tag="KB953_000252"
     CDS             5158..6549
                     /locus_tag="KB953_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HBB6865258.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            7160..7528
                     /locus_tag="KB953_000253"
     CDS             7160..7528
                     /locus_tag="KB953_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HBB6865259.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            7708..8649
                     /locus_tag="KB953_000254"
     CDS             7708..8649
                     /locus_tag="KB953_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HBB6865260.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            8718..9449
                     /locus_tag="KB953_000255"
     CDS             8718..9449
                     /locus_tag="KB953_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HBB6865261.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            9593..11773
                     /locus_tag="KB953_000256"
     CDS             9593..11773
                     /locus_tag="KB953_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBB6865262.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            11793..12275
                     /locus_tag="KB953_000257"
     CDS             11793..12275
                     /locus_tag="KB953_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HBB6865263.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            12291..13052
                     /locus_tag="KB953_000258"
     CDS             12291..13052
                     /locus_tag="KB953_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBB6865264.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            13183..14517
                     /locus_tag="KB953_000259"
     CDS             13183..14517
                     /locus_tag="KB953_000259"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBB6865265.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            14824..15309
                     /locus_tag="KB953_000260"
     CDS             14824..15309
                     /locus_tag="KB953_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HBB6865266.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            15302..15985
                     /locus_tag="KB953_000261"
     CDS             15302..15985
                     /locus_tag="KB953_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBB6865267.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            16111..18756
                     /gene="polA"
                     /locus_tag="KB953_000262"
     CDS             16111..18756
                     /gene="polA"
                     /locus_tag="KB953_000262"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBB6865268.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            18801..19637
                     /gene="mutM"
                     /locus_tag="KB953_000263"
     CDS             18801..19637
                     /gene="mutM"
                     /locus_tag="KB953_000263"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HBB6865269.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            19601..20230
                     /locus_tag="KB953_000264"
     CDS             19601..20230
                     /locus_tag="KB953_000264"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBB6865270.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            20282..20614
                     /locus_tag="KB953_000265"
     CDS             20282..20614
                     /locus_tag="KB953_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBB6865271.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            20926..21417
                     /gene="nrdR"
                     /locus_tag="KB953_000266"
     CDS             20926..21417
                     /gene="nrdR"
                     /locus_tag="KB953_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HBB6865272.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            21440..22813
                     /locus_tag="KB953_000267"
     CDS             21440..22813
                     /locus_tag="KB953_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBB6865273.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            22813..23739
                     /gene="dnaI"
                     /locus_tag="KB953_000268"
     CDS             22813..23739
                     /gene="dnaI"
                     /locus_tag="KB953_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HBB6865274.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            23868..24308
                     /locus_tag="KB953_000269"
     CDS             23868..24308
                     /locus_tag="KB953_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBB6865275.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            24452..25153
                     /locus_tag="KB953_000270"
     CDS             24452..25153
                     /locus_tag="KB953_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HBB6865276.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            25150..26271
                     /locus_tag="KB953_000271"
     CDS             25150..26271
                     /locus_tag="KB953_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBB6865277.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            26286..27518
                     /gene="pepT"
                     /locus_tag="KB953_000272"
     CDS             26286..27518
                     /gene="pepT"
                     /locus_tag="KB953_000272"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBB6865278.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            27650..28558
                     /locus_tag="KB953_000273"
     CDS             27650..28558
                     /locus_tag="KB953_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HBB6865279.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            28593..28865
                     /locus_tag="KB953_000274"
     CDS             28593..28865
                     /locus_tag="KB953_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HBB6865280.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            28876..29922
                     /locus_tag="KB953_000275"
     CDS             28876..29922
                     /locus_tag="KB953_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBB6865281.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            30173..32782
                     /gene="clpB"
                     /locus_tag="KB953_000276"
     CDS             30173..32782
                     /gene="clpB"
                     /locus_tag="KB953_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBB6865282.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(32933..33619)
                     /locus_tag="KB953_000277"
     CDS             complement(32933..33619)
                     /locus_tag="KB953_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865283.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(33597..34091)
                     /locus_tag="KB953_000278"
     CDS             complement(33597..34091)
                     /locus_tag="KB953_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HBB6865284.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(34111..35331)
                     /locus_tag="KB953_000279"
     CDS             complement(34111..35331)
                     /locus_tag="KB953_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB6865285.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            35478..37313
                     /gene="lepA"
                     /locus_tag="KB953_000280"
     CDS             35478..37313
                     /gene="lepA"
                     /locus_tag="KB953_000280"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBB6865286.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            37509..38420
                     /locus_tag="KB953_000281"
     CDS             37509..38420
                     /locus_tag="KB953_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6865287.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            39416..39667
                     /locus_tag="KB953_000282"
     CDS             39416..39667
                     /locus_tag="KB953_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865288.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            39750..40022
                     /locus_tag="KB953_000283"
     CDS             39750..40022
                     /locus_tag="KB953_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865289.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            40112..40351
                     /locus_tag="KB953_000284"
     CDS             40112..40351
                     /locus_tag="KB953_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865290.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            40498..44781
                     /locus_tag="KB953_000285"
     CDS             40498..44781
                     /locus_tag="KB953_000285"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HBB6865291.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            44880..45245
                     /locus_tag="KB953_000286"
     CDS             44880..45245
                     /locus_tag="KB953_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865292.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            45247..45699
                     /locus_tag="KB953_000287"
     CDS             45247..45699
                     /locus_tag="KB953_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865293.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            46313..47623
                     /locus_tag="KB953_000288"
     CDS             46313..47623
                     /locus_tag="KB953_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HBB6865294.1"
                     /translation="MYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLV
                     RMLIDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGH
                     LLSEVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPN
                     MLIAGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEE
                     MIICLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDME
                     ARNRVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYL
                     MTFGEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYD
                     FTVKEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            47646..47957
                     /locus_tag="KB953_000289"
     CDS             47646..47957
                     /locus_tag="KB953_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865295.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            48317..49573
                     /locus_tag="KB953_000290"
     CDS             48317..49573
                     /locus_tag="KB953_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBB6865296.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            49570..50115
                     /locus_tag="KB953_000291"
     CDS             49570..50115
                     /locus_tag="KB953_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865297.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            50116..50616
                     /locus_tag="KB953_000292"
     CDS             50116..50616
                     /locus_tag="KB953_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865298.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            50697..51253
                     /locus_tag="KB953_000293"
                     /pseudo
     CDS             50697..51253
                     /locus_tag="KB953_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            51273..51617
                     /locus_tag="KB953_000294"
     CDS             51273..51617
                     /locus_tag="KB953_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865299.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            51636..52547
                     /locus_tag="KB953_000295"
     CDS             51636..52547
                     /locus_tag="KB953_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBB6865300.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            52626..53618
                     /locus_tag="KB953_000296"
     CDS             52626..53618
                     /locus_tag="KB953_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBB6865301.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            53615..53833
                     /locus_tag="KB953_000297"
     CDS             53615..53833
                     /locus_tag="KB953_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865302.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            53846..54256
                     /locus_tag="KB953_000298"
     CDS             53846..54256
                     /locus_tag="KB953_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865303.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            54308..56836
                     /locus_tag="KB953_000299"
     CDS             54308..56836
                     /locus_tag="KB953_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBB6865304.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            56852..59062
                     /locus_tag="KB953_000300"
     CDS             56852..59062
                     /locus_tag="KB953_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865305.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            59070..60161
                     /locus_tag="KB953_000301"
     CDS             59070..60161
                     /locus_tag="KB953_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBB6865306.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            60182..60724
                     /locus_tag="KB953_000302"
     CDS             60182..60724
                     /locus_tag="KB953_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865307.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VGQEAQKIDAIKIYYVRSAY"
     gene            60919..61176
                     /locus_tag="KB953_000303"
     CDS             60919..61176
                     /locus_tag="KB953_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865308.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            61190..61522
                     /locus_tag="KB953_000304"
     CDS             61190..61522
                     /locus_tag="KB953_000304"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865309.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            61567..62115
                     /locus_tag="KB953_000305"
     CDS             61567..62115
                     /locus_tag="KB953_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBB6865310.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            62122..62574
                     /locus_tag="KB953_000306"
     CDS             62122..62574
                     /locus_tag="KB953_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865311.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
     gene            62671..63006
                     /locus_tag="KB953_000307"
                     /pseudo
     CDS             62671..63006
                     /locus_tag="KB953_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313007.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(63731..64402)
                     /locus_tag="KB953_000308"
     CDS             complement(63731..64402)
                     /locus_tag="KB953_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBB6865312.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
BASE COUNT        21768 a        10414 c        13795 g        18486 t
ORIGIN      
        1 gtttgttttt gcaccgttag aaggagaaga cctcgcttca tttctggttg gtgcggcaat
       61 catgatgatc ggaatgacgc tctttttgtt tggagcagat tattctatga tggaagtcgg
      121 agatctagta gggaaatata tgatcaaaaa gaaaagtttg accgttttgg tcagtttggg
      181 atttgctatc gggatcgtga tcacgattgc tgaaccttcc gtgcaagttt tgggacaaca
      241 agtatatgat atatctgacg gagagattgg cagagtgttg ctgattggga tcgtcagcgt
      301 cggcactgga gtatttctag cctttgcgct gcttcgggtc gtattcaagc tatcctatta
      361 tcaattgatg gctataggct acataggcgt tttgatcgcc tcatttttca ccagttccga
      421 atttatgccg attgcttttg attcgggggg agtgactact ggtccagtaa ctgttccatt
      481 tatcttggcc cttgccggag ggatgactag tatgatcaaa caaaagaaaa atgaaaatga
      541 cagttttgga atggtcggta ttgcttcgtt agggccgatt cttgccgtga tgattttggg
      601 ggtgatcttc caatgagttt ctggcagcaa gtgatagatg gaatcgaaat cgttaccatg
      661 gaagtcattt tggcagtggc acctatcgta atcctatttg ttttaatgaa tatcattagt
      721 tttcgtatgc gcaaaaagcg tttctttgac attatgaaag gtttcttgat aacgactatt
      781 gggttgatcc ttttcctgca cggagtcaat atggcttatg taccggtagg acagcattta
      841 ggcagtgcta ttgcgggatt gccttacaat tatatactgg ttccccttgg ctttttaatg
      901 gggttcttag tagggtttgc tgagccggcg attcatgtaa tgatcaaaca ggttgaagaa
      961 ttgagcgagg ggagaatccg atcgaaaatc atgctgatgg taatttcaat tgggatcggc
     1021 gcagcagtag gattgtctat gtggcgtttg ctcgtcggat tctctcttta ttatttcttg
     1081 atcccaggat acgggctagc atttatttta ggacgaaaag tggacaagat ctttttagca
     1141 atggcttttg ataacggtgg agtagcaacg ggcccgatgt gttcgacatt tattttatca
     1201 atgagcgtgg caatcgctgc acaaattgaa ggaagagatc cgatcattga cgggtttggt
     1261 gtggttgcaa tgattgcatt gacaccaata ttatccactt tagtactagg atatatttat
     1321 aaacgaaaag atgaaaaaga cagaatcgaa aaagaaagat tgcaaaaatt gggggaaagc
     1381 aaatgaaaga tcttgtagca ttgaatcttc acatgattgt gacgatcgtt gacggaggaa
     1441 tgggcgaaga tgttatcgac ttttcaaaag aagcaggtgc agcaggcgga acgatcttac
     1501 atggaagagg ttctggtgta catgattcag ggaaattttt aggtttggac atttcgccag
     1561 aaaaagatat tgtgttgaca ttagtacccg atagtttgac gaataaagta atggatgcga
     1621 ttggtcaagg catcaacatc gatattccag gtaatggtat ttgttttagt attgatgtag
     1681 acaaagtcat tggtatcact aagattcatc aatatcgaga aattatcgga atgcaagaac
     1741 ttttagatga aaataacgat agagaaaagt ctgaatcata gacaagcgtt tgaagaggaa
     1801 ataaacacaa acaagaaggc ggatgagcgt tatttccgct tttttgtttg tatttttctc
     1861 gtgaatactt atgaaatagt aaatttttat gaaattctac tctaagcatc agactttctt
     1921 ttcaaaatat gctattttaa aaatgatgaa aaaaggggga gaaaaatgaa ttttttagtt
     1981 gttttaaaag atactctgat tgagcgattt acatttctat atgttcaatt gcaggaaaat
     2041 aaaacattgc tgcatttttc accagagttc catctagagg ggctaacatt aggagaaatc
     2101 tatcaagcag aagggctgtc ggctgttttg cactttttta aggtagaatt ggaattacca
     2161 gtaaaagatt atatcgagct ttctcttgaa tcttgggatc gagcagtggt tgaactgttt
     2221 ccagaaggtt taatgatcga tacaaacgat gggaagatcc atctgactgc agcagaactg
     2281 caaaaacaaa tgcgttatca aatagatgac gaaaattctt tttctatttt tagacgtcag
     2341 caaaagattc taaaaagctt tttaaaagtg atcagtaaaa aacggaatct gatcaaaacg
     2401 acacagctgt tgaaaaaata tccaggtctg cttcacacgt ctattcaatt tcctcaattg
     2461 attacgatga tccatcgttt tatacgtatg caacagctac catctaagaa gctgtttctt
     2521 ccattgacgg acacatttcg agtagtcaac agagaagata aaaagaaagt gattgtgatt
     2581 gactttacca gaaatcggaa tgtccttcac caagttgcaa tggaaaaggc caattaaggc
     2641 tttttctttt tggatttttt tgtcataata aatattagat gatggaggag ataatatgat
     2701 gcaaacatgg ttagaaaggc taatacgatt tcagcagaac tatcacaaac ttatgcaggg
     2761 aagatatgcg aaatttgatc agttgaatcg cggtctattg atcgctgcgc ttgtctttag
     2821 tttgctaagt cgttggttgc cctatagaat cggacaattg ttgttcatcg tctttttcgg
     2881 atggatgata tttcgtttcc tatcaaaaaa aatctacccc agactaaatg agaatcaacg
     2941 ttatttgaaa tatcttgata gagtcaaaga aaaatatcat cagataaaaa acaaaaagca
     3001 agtcaaaatg gatcgaaaaa gttatacttt ttttgagtgc ccgaattgtc atcaaaagca
     3061 gcgtgcacct aaaggaaaag gacgaatacg tgtgacatgc aaaacatgtg gtataaaatt
     3121 cgaaacgaat gtttaaggga gaaaagaatg aaattagtat tattacgtca tggcgaaagc
     3181 gaagcaaatt ttgaaaatta ctggacagga tggctagatg tcgccttaac agaaaaagga
     3241 aaagaacaag cagcagttgc cggtcaaaaa atgcttgaag ccggacttca attcgatgcc
     3301 gtttatacct ctgttttgaa acgtgcgatt cttaccggac aagtcgcact agaagaaatg
     3361 ggtcagttat ggcttcctat aataaaaaca tggcgtctaa atgaacggca ttatggggat
     3421 cttgtgggta aaaacaaaga taagatgaaa gaaatatacg gaaaagatca agtaaaaaca
     3481 tggcgcagag gatatacgga agttccgccg ttaaccgcag aaaatagatt tgaccgcaga
     3541 tatgatcact tggatccgca tttgatccca tacggagaaa gtctggaaat gacagtgaat
     3601 cggatcattc ccttatggca agatcatcta gcaccaaaac taaaagatcg gcaagattta
     3661 ctagttgtgg gtcatggtaa tagcatccga gcactgacaa aatatttaga agatgttcct
     3721 gaagatcaaa tggatacgat cgatatccca aatgcacaac caatccagta tacattagca
     3781 gatgatttga caattctgga caaaaaaata ctttaaagaa cacctttatc taaaagtgag
     3841 agatagaaaa aagaagcaaa ggaatggaac aaaaggaaca tgaaaaatta tatatgggac
     3901 tttgatggta cattgtttga tacgtatccc gcaatggtag atggtgcatg gcaagcacta
     3961 aaagattttg gtatcagtat ggacaaaaaa gaaatctact tcaaaatgaa aaagtattct
     4021 actagttatt tgatcaatga atcaaacttg aatgccagag agtttaatga attatttcac
     4081 cgatatgaaa aagaaagtac ggaagtttcc cgtccatttc cagaaacgaa acaagtactg
     4141 gaaatgctaa aagacaacgg cggtcgtcat ttcatcctga cccaccgttt gacagaatca
     4201 acatggggcc tgttaaaaga acatcgattg gcacatctga ttgaggaagt agtagggatc
     4261 gatcaggact ttcctcgtaa acctgatcca gcatctttaa attatttgat cgatactttt
     4321 catttagaaa gaacagacac gatgatgatc ggcgatcgtc gtttagatat cgaagcaggg
     4381 aaaaatgcag gagtcgctac ttgtctttac gacatcgatc attttctggg tgagatcccg
     4441 gcagattatg tggtaggaaa tctgaatgaa atccttacct tgcagagcat cattaaaaag
     4501 tagtatcccc tttcaaagga gatatgttat aattttcata aaggagtgat aaaattatat
     4561 ggaaatcaga agacaaaaag aagttgtaga agcatttcat tatgatatga gattgccgaa
     4621 ccaagaagta gaaacagatt tgaaagtagg cttctcgcct ttaaaagaaa cgatcgaaaa
     4681 ctatccgaaa gaacatagca tcgtaggagc acgcttagag ttccgtttag tgtttgatac
     4741 atatgtttta tctggaagtg tgagtcagat caatcatatc ctcaatcgga aaattgaagg
     4801 tcaaaatgat gtcacacaag aagaagtgga cgagctagta gcaccgttat tcgatatcgt
     4861 acgccgaatg tcttatgagg tgacagagat cgccttagat cagcctgggg taaaattgaa
     4921 ttttcaatca gaaaaacaag aatagacagt aaaaacatac ctgcgataga ttaaagctat
     4981 cacaggtatg tttttttatt actaattgta agcaaaaaga atgtcatttc gataaaaatc
     5041 gtctaaaata taaaagtcac tgaaaaaaat tttttcagga ggatggttga agaatcattc
     5101 tcgggaacct ttacttcctt cgatggactt gtcattaata atatgaggag gagaaaaatg
     5161 acaacactca tcacagtttt agtggttcta gtttttgcgg cattattata tgtgttctat
     5221 cgtatgcaaa agcgccatac aaaattttca acacgcgtct ttacagcatt aggagttggt
     5281 atcgtctttg gcggtctgct tcaattgatc tttggtgtag acgacaaagt cgttactcaa
     5341 tcaatggaat ggatcgggat tgtaggtcaa ggatatgtat cattcttgca aatgctagtt
     5401 atgcctttag tgtttatttc gatcattggt gcattcacaa aaatgaaagt ttctgaaaag
     5461 ctagggaaaa ttagtgcgac tgtattgact tctttacttg gaacaacagc tattgcagca
     5521 ttgatcggaa ttggttcaac aatgctgttc ggactccaag gagctaaatt cacacaagga
     5581 gctgcagaaa cagcacgaat ctctgaactg gcaacacgtc aggaaacagt tgaaaactta
     5641 tcgattccac agcagatttt atcctttatc cctaaaaacg tttttgctga tttagcaggt
     5701 agtcgtgcaa caagtacgat tggagtcgtt attttcgcgg catttgtcgg aatcgcttat
     5761 ctaggcgtga aaagaaaagc gccaaaagaa gcagcattct tttcagattt agtcgacagt
     5821 ctgtataaaa tcattatgcg gatcgtgacg cttgttttgc gtttgacacc ttatggagta
     5881 tttgctttga tgacaaacgt tttggcaaca agtgattttg cagctatcgt caatttaggt
     5941 aaatttgtcg ttgcttctta cgtagcattg cttgctgttt tgattgtcca ctcactgatt
     6001 ttgattggtg caaaagtcaa tccaatcaac tatttcaaaa aagcctttcc ggtattgagc
     6061 tttgcgttta cttcacgttc aagtgctgga gctttgccat tgaatatcga gactcagaca
     6121 aaagctttag gagtagatga tgcaacagct aatttctcag caagttttgg tttgtctatt
     6181 ggacaaaacg gctgtgctgg cgtttatcca gctatgttgg caacgatcgt cgcaccagca
     6241 gctggagtga atgtctttag tttggaattt attttgatgc ttgtggcaat tgtaacaatc
     6301 agttcttttg gtgtagctgg agtcggtggg ggcgcaacat ttgcttcact gatcgtttta
     6361 ggggcaatga acttgccagt agcaatcgtt gggttagtta tctctgttga accattaatc
     6421 gatatggcac gtacagcagt aaacgtcagc ggtagcatgg tagctggtat cgtgacaagt
     6481 gcacgcatca aagatttaga tcgcgatgtc atcaatgatg attctaaagt gatcgatgtg
     6541 aatgcatagt caaaaagagg ttgtgataga agtggtcagc tttaagaaat aagaagaagt
     6601 ttcctaaaaa tagtttctca aatttttgtg aaatttcggc ttatttccga gaagctactt
     6661 ctgaaacacc gtgaataaga ggttgtgaaa aagctgccct gcatcaagta ataagaacag
     6721 ccaaacaaaa atagttcctc atattttacg tttggttgag cttattaccg cagatgcaag
     6781 cttttgaaca ccgtttaaga aagaggtcgt gagcctgccg tttcactctg agtcacaaag
     6841 agcaatttca aaaaatagct tctcatgttt gttgaatatt gtgtcttgtg accgaggagt
     6901 tgtcaggaga acaccgttta ccagaggttg tgacttgttt ttagtcacga cctctttttt
     6961 tatttattcc atataaatac acattattta ttccgaaaag tgaaagcgca aataacaaaa
     7021 acaaccaact tatttattat taaagaaaag cgtatttaag taggacgatt ctgttgagag
     7081 ctttcgtaga ttttcccatt ttcctatggt aaactgacaa agttgagaat gttagaacag
     7141 atcgtttagg aggaaacaga tgtttacgaa tgaaataaaa atcatgcttt atgtggacga
     7201 tgtagaaaaa aatgcaacgt tttggagagc agttggcttt gcggaaaaag atcgtcaaga
     7261 aatggatggg acgcttataa ttgaagtagg agttgaggac agtcaaacct catttctgtt
     7321 atatgatcga gcatttgttg agtcccattc acctggcaca gctactagtg ccccatcttt
     7381 gatgttttac agtaaagata tctttggact atataaaaaa atgcagacgc aatcagtcac
     7441 attaggtgat atggtacagc ttggagaaga atatgtcttt aactttgctg atcccgatgg
     7501 caactacttt gcggttacag gtaaataata gaaataaaga ctttgttttt ctgtgcgaaa
     7561 caggaaagcg aagtcttttt aattcaaata ataatattat gtaaacaaaa aactttccat
     7621 gcgtaattat acacaagcaa ataaatcgtc aaatgcgaac gccttcttta tactgtttag
     7681 gaacgaaaag aaggaaggaa gaaatacatg gattatcaag tactgcttta ttacaattat
     7741 acaaaaatcg aagaaccaga ggaatttgcc aaaaatcatt tagccttttg caaatctttg
     7801 gatcttaaag gtcgtattct agtagctgaa gaaggaataa atggtactgt gtctggaacc
     7861 gttgaaaaca cgactgccta tatgaaagca atgaaagctg atcctcgatt tgctgatacg
     7921 atgttcaaga tcgatccaag cgaaggacat gcctttaaga agatgtttgt aagataccgt
     7981 ccagagcttg taagtttaaa gctagaagag gacattgatc caaaccaagt gacaggtgcc
     8041 tatctttcac cgcaagaatt taaagaagct attttagatg aacagacagt ggtgattgat
     8101 gcacggaatg attatgaata tgacttggga cattttcgag gggctgtacg cccagatata
     8161 cgcagttttc gagagcttcc agactggatc cgcgagcaca aagaagaatt tatggaaaaa
     8221 cgggtagtga cctactgtac tggtggcatt cgttgcgaga agttttctgg ttggcttgtc
     8281 cgtgaaggat tcaaagatgt cggacagctg catggcggta ttgctactta tggtaaggat
     8341 cctgaagtcc aaggagaatt atgggacggc aagatgtatg tgtttgatga acggatcgct
     8401 gtcgatatca accaagtaga taaacaaatc gtcggtcgcg actggtttga cggaacacca
     8461 tgtgaacgat atatcaattg tagcaatcct gagtgcaatc gtcagattct tgcttccatt
     8521 gaaaatgaag aaaaacattt aggagcatgt agcatagagt gtgcgaaaca tccgcgcaat
     8581 cgctatgttc aagaccatgg aatctctcca gaagaagcag aactgcggtt tacagcttta
     8641 attaaataga aaacaatagt tttgattgtc gaccatttcc aatatgcgct atactagcgt
     8701 tgaggatggt gatccacatg aaatcattga tttatgctat tggggatgtc catggcaaac
     8761 atgagctttt tgaaaaactt attcactatt atgatccaaa gatccatcag cttgttttat
     8821 taggggatct caatgatcga ggtcctcgta caaaagaatg ttttttatta ggaaagcaat
     8881 tagtcgaaga aacaggagcc atttacctcc ggggaaacca tgaagaatat tttttgcaat
     8941 ttctgcaatc accggaggac tggtatgcgc cttatatgag aaatggtgga aaagaaacgt
     9001 ttgaaagcct gattcaccca ggggcatcgg aggaatactc cccaactgaa atctcgatga
     9061 tgatccaatc gagatattca gaactgatta aatttttagt ccagcgcccc ttgtattatg
     9121 aatgggaaaa ttatcttttt gtccatgccg gtgtggattt gacaaagaaa gactggcgta
     9181 agacaagtct aagagacttt atttggataa gggaaccatt tcatgaaggg aaaaacaata
     9241 caggtaagac aattgtattt ggacatacga ttacaccaat gctccatgga gatatgcaga
     9301 caacagacct ctggattcag gatcataaaa tcggaatgga cggtggggct gtattcggag
     9361 ggtcattgca tggggttgtt tttgacaagg aaggtattct tcaagatatt gaatatcaga
     9421 acaacgaagg accttggcat ggaatctgac taatggaaag aatgaaggag ctgtgacttt
     9481 gttgcggctc tttttatttt tatagagaat attgtttctt tcagtagttt aacccaggat
     9541 aaaaagtatg atagaataaa gaagcaattt ttagcagaaa agaggcattt taatgacgga
     9601 aaatttgaac cagacgatca ttcagcttgt acaaaaagaa ctttcagact atcgtcctaa
     9661 gcagttgact actgtactta atttgttaaa tgaaggaaac actgttcctt ttatcgcgcg
     9721 ttaccgaaaa gaaatgactg gaagtcttga tgaagtccag attcgtgaaa tcgaagagcg
     9781 gtatgcctat cttgaaaact tagaaaaacg taaaactgaa gtgattcggc taatcgatga
     9841 acaagggaaa ctgactcccg agttagaagc agaaatcacc caagccgtga aaatgcaaca
     9901 agtggaagat ttgtatcgtc catacaaaca aaaacgacgg acaaaagcaa cgattgctaa
     9961 agaaaaagga cttgaaccac tcgcgaagtg gctgatgcag cttacagatg gagaggtgca
    10021 atctgaagca gaaaaatata ttgacaaaga aaaagaagtc tcttctgccg aagaagcgtt
    10081 gcacggtgct catgagatta ttgctgaaca agtcagcgat aatgcaaaat ttcggacttg
    10141 gattcggtct tatacgtata acaaaggtat gtatgtcagc aatgtcaagg atgagcaagc
    10201 agatgaaaaa ggcgtatatg aaatgtatta tgactttgca gaacccgtcc ataaaatggt
    10261 ttctcatcga atattagcta ctaatcgtgg agaaaaagaa gatattctga aagtattctt
    10321 tcaagtagat gaagcagcaa ttttagctta cttggatcgc caaatagtca aaaatcctgc
    10381 ctctccttct tcttcattcg tcagagaagc ctatcaggat agttataaac gctttattca
    10441 gccagcaatc gagcgagaac tgcgcaatga attgactgaa aaggcagatg agcaagcaat
    10501 cgctattttc ggcgaaaacc tccgtaatct tttgttgcaa ccgccactaa aaggtaaagt
    10561 tgtcctggga tttgatccgg cttatcgtac tggatgtaaa ctagcagtcg tagatgcgac
    10621 agggaaagtt ttagcaatcg aagttattta tcctcataaa cctgcagcgc aagcaaaaag
    10681 agaagctgct ggaccagctt ttatccaatt aattaataaa tatcaagtag atatggtagc
    10741 tatcggaaat ggtacagcca gtcgggaatc tgaacttttt gtggctgagc agttgaaatc
    10801 cgccgaccat aaaacctatt acgctatcgt caacgaagca ggcgcttctg tttactccgc
    10861 tagcgaaatt gcacgaaaag aatttccgca tctacaagtt gaagaaagaa gtgctgtttc
    10921 tattgcccgt cgcctgcaag atcctttagc tgaattagtc aaaatcgatc ctaaagctgt
    10981 cggagtaggc cagtaccaac atgacgtttc tcaaaaacgt ttggctgaac aattggattt
    11041 tgttgtggaa acagcggtta accaagtagg tgtggatgtg aatacagcaa gcccgcagct
    11101 cttgcagcat atatcaggat tgaataaaac aactgcccaa aacattgtca tttatcgtga
    11161 agaaaacgga gaattcaccg cacgaactca attgaaaaaa gtgccacgct taggaccaaa
    11221 agcatatgaa caagcaatcg gattcttgcg agtccctggt gggaaaaaca ttttggacaa
    11281 cacaggtatc catcccgaaa gttattctat tgcaaaagaa atattaactt cagtccaact
    11341 ttcagaaaaa gagcttggta cagaagaagc agtagaaaaa ttgacacgat tatccgtaga
    11401 gaaactggct gaaagcctat cagtcggaga agaaacgtta gccgatattt tagctgggct
    11461 gacacaacct ggacgagata tgcgtgatga gatgccagcg cccttattac gtacagatgt
    11521 attgagtatg gaagacttga aaccagggat ggaattgact ggaacggtcc gtaatgtcat
    11581 tgacttcggc gcatttgttg atatcggtgt gaagcaagac ggacttgtcc atatatcgaa
    11641 actcagcaaa aaattcgtca agcatcctac agatgtcgtg tcagtaggag atatcgtaac
    11701 agtttggata gaacaagtcg atacgaaaaa aggcagaatc agtttgacga tgctttcccc
    11761 ttatgaagag taacaatgag gagtaacaaa aattgacaca agaagaactg cagcagttag
    11821 tagaagagat atcattaaaa agcttccagc ttcctttcaa gcaccaagca atgttcaatc
    11881 cacgcttacg tacaactggc gggcgctatc atttaaacga tcatcattta gattttaatg
    11941 cgaagctatt tgaagagtca gacgaagaga cgattgcagg aatcatcaaa catgaacttt
    12001 gccattatca tcttcatcta gctggaagag ggtatcagca taaagatatg gatttcaagt
    12061 atcttttgaa aaaaacagga gggttgcgct atgcaccagc cttgacgaag aaacagataa
    12121 agatggagta ttatcgatgc caagattgtt ctgccacgat ctgccgcaag agaagaatcg
    12181 atgtacgcaa atatcgttgt ggaagatgtc atggagaaat caaatggatt gagacaaaaa
    12241 gagaagactt taatgaagca caaaaaatca gatgaaagga tagaaggcct ttggaaaaaa
    12301 gtgagcagag gaatctaaaa gactggaatc agctattaaa ttttttagcc gcacctgaag
    12361 agggaaattt tgaggaactg acggcaccta cacttatttt agctggaaac tgtcttccta
    12421 tcttagctga taaagcagca caaatgtatt taaatggcca agtagatcaa ttgtttttgg
    12481 tcggaggggt ggggcatgcg actagaatcc tttatgagaa ttttgaaaag caaggatttc
    12541 attttgaaga tggcatgagt gaaagtgaaa tctgtcgtca gtatctaaaa gaagtctatg
    12601 atttgcccga taaggctttt ctgatagaat cgaagtctac aaattctgga gagaacgcca
    12661 ctttttcact ggagattctt cattccttag gagctgtacc agaaaaaatc ttattgatga
    12721 atgatccgac actgcaacgc aggactcgag caacttttga gaaagtatgg caaaatgaac
    12781 aaactatttt cgcgaatgct gtgccatttg tgccggaaat ccttcacttt tctgaagaga
    12841 tcatctttac agcaaaagaa ctgaatcatc aatggcctaa ggaatatttc catgcgctag
    12901 tattaggaga aatggaacgt ctccatgacg atgagaacgg ctatggtccg aaaggaaaag
    12961 attttattcc tcacattgat atatcagagg aagtttggtc ttcttacacc agaattaaag
    13021 agtcaataaa aacggatttt tctcgtactt gaccatttta gcaagcgaat cctcctttcc
    13081 aaataaattt ggttgaaatt ttcaatcgtt tataagataa tgaggagcgt gttgaatgta
    13141 atagctaaaa gaatggacgc tagataagag aagaggattc aaatggaaaa tcaaaataaa
    13201 ttgtatcatt ttgttggtat caaaggttca ggaatgagct ccttggctct tgttttacat
    13261 gaacaaggtc taaatgtcca aggatcagac attgaaaaat atttctttac acaaagagat
    13321 ttagaaaaag caaatatcac gattctgcca tttaacgcag ataatgtaaa accagggatg
    13381 acgatcattg caggaaatgc atttcctgat tcacatgaag aaatccagcg agcaaaagaa
    13441 ttaggtttgg aagtgattcg ctatcacgac tttattggtc attttatcca aaattatacg
    13501 agtatcgctg taacaggctc tcacggaaaa acaagtacga ctggactgct ctctcatgta
    13561 ttgagtggtg tccgtccaac aagttatcta attggagatg gaacaggaca tggtgatccg
    13621 caagcggaat tcttttcatt tgaggcctgt gaatatcgcc gtcatttcct tgcctattca
    13681 ccagactatg caatcatgac caatatcgat tttgatcatc cggattatta tacaagtatc
    13741 gacgatgtat ttacagcttt ccaaacaatg gctggacaag tgaaaaaagc tatttttgcg
    13801 tatggtgacg atgcttattt acgcaaatta aaagcaaatg tgccgattta ttattatggt
    13861 gtcacagaaa acgatgatat ccaagcacga aacatcgaac gcacaacttc tggttctgct
    13921 tttgatgtct atcatgggga tgaatttgtt gggcacttca ctgtaccagc attcggtaaa
    13981 cacaatatct taaatgcttt aggtgttatt gcagtagctt atttcgaaaa actggatctg
    14041 aaagaagttg cagaagaaat gctgactttc ccaggagtaa aacgtcgatt cagtgaaaag
    14101 atcgtagcag acatgaccgt agtcgatgat tatgcgcatc accctgcaga gatcaaagca
    14161 acgattgatg gcgcacgcca aaaatatcca gataaagaaa taattgcagt tttccaaccc
    14221 catacgttta cgcgtacgat tgcattgatg gatgaatttg cagaagcact tgatttagct
    14281 gataaagttt acttatgcga tatttttggt tcggctcggg aagaacaagg aaacgttaag
    14341 attgaagacc ttggagcgaa aatcaaaaaa ggtggagaag tcatcaaaga aaataatgta
    14401 tctccattgt tagattatca tgatgcagta gtaatcttca tgggtgctgg agatgttcaa
    14461 aaatttgaac aagcgtatga aaaactttta agcagcacga cgaaaaatgt actgtaatac
    14521 aattactgca gtgtccttat aaaaatagaa agagggggag tggcaaaaat cttgccaagc
    14581 cccctttttt gcaacttatg ataaacggtg ttcaaaagct tgcatctgcg gtaataagcc
    14641 caatcaaatc aaaaacatga gaagctgttt tttcttggct gttcttatta tttgatgcag
    14701 gacagctttt tcacgacctc tgtaaactat aggaatctgt taaatcaagg gagcagaact
    14761 tgccttttac gctacttttg gtacaataat gtatgattaa aaataaactg gagggtttct
    14821 atcttgaaca taggtaagcc gagaaaacta ggaaaaacaa taaatgaaat cgaggaaggc
    14881 gactcgttat ccttaacaga atctattgaa gataaccagc ttttgctgta tttgggattg
    14941 acgaatgatg caaatccttt gtatatccaa catgactttg cacaaaaaac agaatatcaa
    15001 cagccaatcg ttccatcagt gatgctgatg gggatcatca caagtgcaat ttcaaagcat
    15061 cttcctggac caggatcaca tgttgtcaat ttttctgtca attttattga accggttttc
    15121 cattatgaaa cactgacttt tcagtttgag gtcataaaag tagataagat gaaggatgtg
    15181 gtgacgattt ctgtagaagc ggagaatacg aacgaagatc gtgtgttgga tgctgtcgtt
    15241 atggtccaac cgccacaagt tcctgaattg gaaatagcaa tcgaaagtga aggtgaattg
    15301 aatgaataat ccaagtacat caatgtcagg tatcaatcgt ttttatgcaa aagtttatgg
    15361 atttttaggt atgggacttg cccttagtgc agtcacttct tacttggctc tttgggtatt
    15421 tcctttgcaa ttagcaactt ttatcaataa ttttccacta ggctttgtgg gtctgtggat
    15481 cgttgaactg attttagtaa tggttctagg cgtaaaagca caaaagaatc cgagcttagc
    15541 aatcggtgga tttattgcct actctctttt gaatggcata acattagcgg ttactcttgc
    15601 atactacgat attggaacag taacgcaagc tttcttgaca gctactttga tgttcatggg
    15661 gatgtctgtt gtcggagtaa caacaaaaag agatcttagc ggggttggaa gagcaggaca
    15721 ggcagcttta atcggagtga tcattgcact agtcttgaac atcttcattt tgagaagtag
    15781 cgtagttgag ctgttcatct ccctactgtt agtcgtcatc tttgcaggga ttacagctta
    15841 tgataaccaa agcatccgta atatctatgt ccagacaaac ggaacagcac atagtggaat
    15901 tgctgtattc atggcattga agctgtattt ggactttatc aacttgttct tggcgtttct
    15961 gagaatcttt ggacggaaca actaaaaaag aaaaaaattc atcagttggg gctataccga
    16021 attatttggt atggtcccgc tttttctttt cgcattccgc aggattcttt gttagaatga
    16081 ataagaactt tacagtaagg agttaagaag atgacaaaaa ataaattatt actcgttgat
    16141 gggaacagtg tggcttttcg cgcattcttt gcgcttcaca attctcttga acgtttcaaa
    16201 aatagaaacg gtcttcatac aaatgcaatc tatgcattca atacgatgtt tgaaaatgtt
    16261 atgcaaaaag agcaaccgac tcatgttctg gttgcgttcg atgcaggaaa aacgactttt
    16321 cggacagaaa tgtatgcgga gtacaaaggt ggaagatcca aaacacctgg cgaattcaaa
    16381 gaacaaatgc catatatacg tgatttgttg acaggactag gggtacagta ttacgaattg
    16441 cccaattatg aagcagatga tattatcggt actttggcag aaaaagtaga caaagatcaa
    16501 tttgatgtcg tcgtgctttc aggtgatcgg gatttgactc aattagctag taaagaagta
    16561 aaagtggata tcactgtaaa aggtgtcagc gatatcgaaa gctatacacc agaacatgta
    16621 gcggaaaaat atgatgggtt gacgccaaaa cagattattg acatgaaggg actagccgga
    16681 gatgcctctg acaatatccc cggtgtgact aaaatcggtg aaaaaacagc tatcaagcta
    16741 ttgaaacaat atggctcagt cgaaggaatt tatgaacata tcgatgaaat gaagcaaagc
    16801 aaaatgaaag aaaatctgat aaatgacaaa gagcaggctt tcttatcgaa aaaattggcg
    16861 accatcaata cttcagcgcc tgtggatgtc tctatcgact cattgaagta tgaagggaaa
    16921 aatctcgata aactcgttcc gttttataaa gaaatggatt tcaatcagtt cctttctaaa
    16981 ttgaatatcg tagaagaaga agtaaaaatg gatgatattt tgtttgaagt cgttcatgaa
    17041 ttcaaagagg aaatgtttac tacggatatg gcgttgtacg tggaaatgat gggcgacaat
    17101 tatcatacag aggaaatcgt cggagtagcg tgggggacag aaaagaaaat ttatgtcacg
    17161 aacgatctat ctgtattcga aaaccgagcg ttccattctt ggatcactga tccgactcgg
    17221 ctgaaaaaag tatatgatgc gaaacgaact tatgttgctt taaaccgcta tactggaaaa
    17281 acgaaaggaa tcgattttga tgtcttgcta ggtgcatacc ttctcgatac gaatgaaaaa
    17341 agcacagata tcgaaggaat agctgctcat tacggataca acgatatcca atcagatgaa
    17401 gctgtatacg gcaaaggggc gaaaaaaggt ttaccagaag aagaggaact cttttttgct
    17461 catttagcgc gaaaagtagc tgcaatcaat gcgttgacac caaaattgag ccaagaactg
    17521 gcagaaaaaa accaagaaga tcttttccgg aaaatggaac tgccattgtc tatcattttg
    17581 gcagagatgg aaataagcgg gatcaaagta gatgcgactc gcttacaaga aatgaagggc
    17641 gagttctctg ctcgcttaag agagatcgaa caaaaaatct acgaagaagc cggcgaagaa
    17701 ttcaatttga attcaccaaa gcaattagga gtgatcttat ttgaaaaaat gggattaccc
    17761 gttatcaaaa aaacaaaaac tggatattcc acagccgtag atgtactgga acagcttcgt
    17821 gaacaagcac caatcgtaga ggatatcctc acctatcgtc aaattgcaaa aattcaatct
    17881 acctatgtcg agggactgct gaaagtgatc caatcagatg gaaaagtgca tacccgttat
    17941 gtccagacat tgacccaaac aggacgatta agctctgttg atcctaattt gcagaacatc
    18001 ccgattcgat tagatgaagg gagaaagata cgtcaggcat ttgttcctag agaaaaagac
    18061 tggctgattt attcttctga ctattcacaa atcgagttac gcgtacttgc gcatatctca
    18121 aatgatgaac atttgaaagc tgccttccta gaaggtcaag atatccatgc cagcacagcg
    18181 atgcgtgtat ttggaatcga aaaggcagag gatgtcacac caaatatgcg taggcaggca
    18241 aaagcagtga attttggtat cgtgtatggc atctctgatt acggactttc acaaaatcta
    18301 ggaatcagcc gaaaagcagc acagcaatac atcgatacgt actttgaaaa atacccaggt
    18361 gtcaaagaat atatggaaag aatcgtacgc gaagcaaaag accaaggcta cgtcgaaacg
    18421 ctgtatcacc gccgtcgata tctgccagac atcaattcaa gaaattacaa tatccgttct
    18481 tttgcggaac gaacagctat caatacacca atccaaggaa gtgctgctga tattttgaaa
    18541 atcgcgatga tcgaattgga taaacgatta aaagaaacag gtcttcaagc tacgatgctg
    18601 ttacaagttc atgatgaact tgtcttcgag gtaccggaaa aagaacttga atcattagat
    18661 aaattggtga aggaagttat ggaacaagct gtttctcttc atgtaccact gatcactgac
    18721 agcagttggg gaaaaacgtg gtacgaagca aaataaacat tgtttaagac agagcaatat
    18781 aatgaaggtg aaaagaaaaa atgccagaat taccagaagt agaaaccgtt cgtaaaggat
    18841 tggaacggtt agtagtagga aaaacgattc aaaaagttca ggtcttgtgg cccaagatca
    18901 tcgagcaacc agagacaccc atctttgagg cttctcttgt tggcgaaacc atccaatcca
    18961 ttggacgtag agggaagttt ttaatttttc atctcgatca ttgtgaactg atctcacact
    19021 tgcgaatgga aggaaaatat caatttacga aagaaaatac accaatcgat aaacatacgc
    19081 atgtactttt cttttttgag gatggaagcc aacttcgcta caacgatgta cgaaaatttg
    19141 gtcgtatgac aatcgtcgaa aaaggagcaa gtgcaactta ccgaggaatc atgaaattgg
    19201 gacctgagcc tttgccagat agcttcttat tagcagattt cgcaaatgga ctgaaaaagt
    19261 cccacaaagc aatcaagcca ttgctgctag accaacgtct agtgactggt ctaggtaata
    19321 tttatgtaga tgaagcatta tgggaagcga agatccatcc agaacaaccg gcgaacacac
    19381 tgcgccccaa agaggtagaa caattacgtc tatcgatcat tgatgttttg gatcgtgcaa
    19441 tagaagccgg aggtaccacg attcgcagtt atttgaatgc attaggagaa tcaggcggat
    19501 ttcaagtagc actgcatgtc tatcagcaaa ctggcaaacc atgtatacgt tgtggaacgc
    19561 cgatcgtgaa aacaaaagtg gcacaaaggg ggacgcacta ttgtccgaat tgtcaaaggc
    19621 taaaaatcgc aaagtaggat ttgttttagg tttgacgggt ggaatagcta ccggtaaaag
    19681 tacggcagcc aaagtctttc agtctcatgg atttcctttg atcgatggcg atgtgattgc
    19741 cagagaaact gttgagcctg gaacgcttgc tctgaggaaa attgtatcag tgtttggcca
    19801 agaggtattg cagcctgatg ggcaactaga tcgagggaaa cttgggatga ttgtttttcc
    19861 atcaaaggaa aaacggcaaa aacttgatca gctattagat ccgtttatcc gaaaagcgat
    19921 aaaagatcaa atcgcctctc taagttccaa tcatccttta gtaattgtag atatcccatt
    19981 gctctacgaa ggtcattatg accactatat ggatgcagta gctgtcgttt atactacgcc
    20041 agaaactcag cttcagcgtt tgatgaaacg taatcaactg acgctagaac aagcgcagca
    20101 gagaattagt agtcaacttc caatcgaaga aaaaaagcag cgtgcggata ttttgttcga
    20161 caacaatgga acaaaagaaa tacttgtggg acaaatcgaa aattggcttt ttgctaatcg
    20221 ctttctttga gtatctgtgt caattttgct atcctgagta tatagaaagt gagggatagc
    20281 gatgggatca ttaaaaaatc aaaaggataa agtcgtaggt aacgcaaaag aaaaaatcgg
    20341 cgagatgctc aataaagaaa atctaatcga tgaaggtcgt acgcaatcag aaacagcaga
    20401 agaaaaagaa aagcagtacc aagcaagaat cgatgaaata gaagaccagc aggcagaaga
    20461 agaagcgaag cggcaagatt tgactcaaga acgaaaaaat ccgacacctg atcgtagtaa
    20521 aacaccgctc ccagaaagtg accacattcc cgatgagcaa aaaatcgagg aacagcgaat
    20581 gaaacactat acaggaatcg aagagaagtt ataaaatagt aaaaaatcga gatgtgactt
    20641 gaagcagatg agaaggtgtt catctgcttc aagttttttg tgctagatga ctaaatcagc
    20701 ttttgtgtaa gttatttctc agtaatcaag gtgatcagta ttggaaaatt gaaatttagt
    20761 aaggacaggg gacacaatta cgcatgaaag tagaaaaaat tgttcttata tccgtgtttt
    20821 ttgggtagga cagtcgcgtt tgtgatttgt ttcgtgttat aatacaacta gttgtaaaga
    20881 aagacacatc ctttatataa aatgcgatgc aagaggtgag acaaaatgag atgtcccaga
    20941 tgtcaacata ataattcacg agtaatcgac agccgtcaag cagatgatgg acgtgcgatc
    21001 cgtcgccgtc gcgaatgtga gaattgttcg ttccgattca ctacatttga acgaattgaa
    21061 gctgctccgt tattagtaat caagaaaaac ggggaacgcg aagagttcaa tcgcgataaa
    21121 attttaagag gactcatccg ttcagcagaa aaaagacctg tagctatgga acaaatggaa
    21181 caaatcgtca accatgtcga aaaccgcgta cgcagtctcg gagaaaacga agtatcgacg
    21241 aatctaattg gtgaatatgt gatggaagat ttagttgatt tagacgaaat cgcttatatt
    21301 cgttttgcaa gtgtttatcg ccagttcaaa gatatgtctg ttttcttaaa agagttgcaa
    21361 gatatcgtgg ataaagcaaa agaaaataat aaagagaata ataaagaaaa caattaagaa
    21421 acaaaaagga ggcatttcct tggaaaatgc ttggaaagag cttcaaccaa aaaatattta
    21481 ccaaggaaga aaagcggctc cgctgagaga agacagcaag aacgccttgc tgtatcttta
    21541 tcagccacta atcggcggag aagccttgag tatctatttc accctgttat cagaaatctc
    21601 tcaagaatct ggcaaaggtc ctgaagggat gcatgcagat ctgcttagta gtctaggctg
    21661 cggtatcccg caattttatg atgcaagaaa aaagctagaa ggaatcggct tactggatgt
    21721 ttattttaaa gaagatagtg atctgggacc tcgttttatt tatgagctgt tagaaccact
    21781 cattcctaaa gcatttttca aagactctgt tctggctttt cttcttctgg aaaaagttgg
    21841 tgaacgacga ttccagaagc ttacccaatt ctttgaacct aaaaggcata cagcagaggg
    21901 atatcaaaaa atcacaaaaa aatttttaga tgtttatcgc ttcaatgaag cagtgtacgc
    21961 tgctaatcaa gaaaacatcg aatcgctgaa taaatctttt gaagaatctg aatcagtagc
    22021 attacttgaa gcagattcga cattcgattg gcagtttttg aatgattggc tggcaaaaaa
    22081 acatattgca agtcttgatg atccaatcat caaacaagtc gacatgtatc atcagcttta
    22141 cggtttcgat gaattgacac ttggcgaact gatcagtcaa tcttatgact ttagtgaaca
    22201 aaaagtatct gaaaaagaac tgcaaaaagc cattttggca atgcagagac gtccggtgaa
    22261 gcaaacaaaa gaaacagttg cggtttctga aaacagtaat gaaccctcgt caaacgaaca
    22321 tttgacaaac gcagcgcttc agctgataaa agaagcacag agcattccac cgatgaaata
    22381 tctggaagca atcaagtcag aaaaaggcgg atatgtttct aaacaagaaa actggttact
    22441 gcaagatctt gtttctcaat caggtctttc cagtagcgtg atcaatgttt tgctgaatta
    22501 tgtactagtg atcaagaata atgctagctt aaatgccagt tatgtaaata cagtggcaaa
    22561 tgaatgggca caaaagaaaa tcgccacagc agaagaagcg atccagcaca ttcggcaaat
    22621 aagtaatcaa gcaaaacaat caaaacaaaa gaagcaaaca aattatcaaa ctggcaagcg
    22681 aaatgttcga cgggaaaagc ttcctgattg ggtcaaccag ccaaaagatg aaaaacagat
    22741 cagcagcgag aaaaaagcag agattgaccg ccgttttaaa gaatacctgg caaaaaagga
    22801 gggtgatatc tagtggaaga cgtaggcaaa gaattgaaga ggatcatcag caaacgcaat
    22861 tatcaagaaa agtatgctga aatgatcgct gaagtcttaa atgatgcgga tgttcaagct
    22921 tttctaagcg cacaccaaga agaattgaca caagcggata ttgaaaaaag ttacgctaaa
    22981 ttgtatgaat tcgtgcaaga aaagcgtaaa tatcaaaaaa acgatccaac gatgatcgct
    23041 cccggctatg agccgaagct gacgttgaat tttcattatg tggatgtcac ttatgtacca
    23101 acggaagcat tgttagcacg gcaaagacaa gaagaaatca gaagccgagt aaaagcaatg
    23161 gatatgccaa aagatattca atcggcttct tttgaaaact ttgaacgtac agatggacga
    23221 ggatatgcga gcttagaagc cctagatttt gtagagaaat atactgctga tccaaaaggg
    23281 tatcataaag gactgtatct agtaggaagt tttggtattg gaaaaacata tttattagga
    23341 gcaacagcga gagaattagc gattgctgga ttcaccacga cgctagtcca ttttccgaca
    23401 tttgccgttg agatgaaaca ggcaatcgga aaagatcaag tggcagaaaa gttagatgca
    23461 gtgaaacgtt cacctatttt gatgatcgat gacattggtg cagatgcgat gagtagttgg
    23521 atacgagacg aagtattcgg tgttatcctt caatatcgga tgcaggaaca gttaccaacc
    23581 ttcttctcat cgaattttac aatggatgaa ttggaacagc atttgtccgt cactcaacgt
    23641 ggagatgaag agccgctaaa agcgaaacgg ataatggaac gcatacgtta tttgacaaaa
    23701 gaaatcgaaa tgactggtcg caaccgacgc aatggttgaa cagttgacaa gtccaactga
    23761 catttgacct agtagtaaaa gcaggtcagc gaatcattat atggacagaa atgaccggag
    23821 acaatcaccg caacaagtaa tgcagaaact taaacggagg attttttatg agggctattt
    23881 tttctagtat atctgtagtt ggtctatttt ttcttttagc aggctgcggt agcgcagata
    23941 gcaacacagg ttctgttgat tcgattgatg cgggtcaaac agtaaaaaca agcagtacga
    24001 aaacggagag acaaagcata tttgtcggaa cgattgaaag tatcactcaa gtagaagcag
    24061 aagaagaaac gcttcagttg aaactcgtcg atgtcatggc caaagaagat ccagcgaata
    24121 tgggaacgtc attcagcaat gacggagtga tcttaaatgc tactgctgaa aaaataagcg
    24181 tggacataaa tgaattgaaa atcggtgaca aagtccaatt tacattagtt gagatgccga
    24241 tcatgacgat gtctatccca ccgcaagttc ctggagattc gattattgat gtttcattga
    24301 acaaataacc aataacattt tttcaaaaat aagaaaatgt tgttttatag aacgaaaagg
    24361 tatatttttt aaggagatat gggaattttt ttattcctag tctccttatt ttatttggaa
    24421 aatgctataa ttatctagaa ggagtgaagc tatgaaccat ttagatttat ctcagcgctt
    24481 agccgcagtg ggtgactttg tccctgaatc cgctcgttta gcagacattg gatcagatca
    24541 cgcttactta cccgttgcat tgatattaag aggaaaaatc gaatatgcag ttgctggtga
    24601 agtcgttcaa ggtccttatg aatcagcacg taagcaagtc aaaaaagatg gattagaaga
    24661 ccgtatcacc gtacgccttg caaatggtct agatgctatc cagccacaag atggtattac
    24721 agctgtcacg attgcgggta tgggaggttc actgatccga gatatcctag aagctggcaa
    24781 gttgaatcaa cgcttaagcg gacaagaacg tttgatcctt cagccaaaca tcggagagcg
    24841 tacattacga tcttggctgc aagaaaatca gtataaaatc caatcagaaa aaatacttga
    24901 agaaaataaa aaaatatatg aaatcattgt agccgaaaaa gaacaaaaac cagttactta
    24961 caatgaaatg gagctgatgt tcgggccatt tctgcttgaa gagaaaagtg agatcttcaa
    25021 atcaaaatgg acaagagagc ttcaacaaag aaaagctatc cgtgaacagc tcaaacaagc
    25081 agctaatcaa gaggcacgtc aaaaacaaat cgagcaggaa atccaaatga ttgaggaggt
    25141 tttaaagcca tgacaatcag cggacgcact ttcatccaaa agtttgaaag ctattgtcct
    25201 cagtggctag ctgaagaagg agacccagtc ggactgcata tcggaacatt ggataagcca
    25261 atccaacgag taatgatgac attagacgtt cgtccagaag tggtcgaaga agcaatcaag
    25321 aaaaaaatcg atttgctgat tgctaaacat cctccaatct ttcgtcctgt aaaacggctg
    25381 gttactgatc agccacaaga aaaaatgtat gcagatttgt tgaaacatga tattgccgtc
    25441 tatgcagcac acacgaacat ggatattatt tgggatggac tgaatgactg gttttgtgaa
    25501 cttctcggta ttgaagtgga gagttattta gtaaagactc atgagattca ttataaaaaa
    25561 ctggccgttt atgtacctgt ggatcatgca cagaaaatgc gtgaagtatt agctgcagct
    25621 ggtgctggta cacaaggtga ttataccggt acaagtttta cctcgattgg acacggtcga
    25681 tttactccgg aagctggtgc acaaccagcg attgggaaag tagggaagac agaacaagtt
    25741 caagaggcaa aagttgaggt tattttgccg gaaactatcg aaaaacaagt gattcaagcc
    25801 atgcggtcag ctcatcctta tgaggaacct gcttatgatc ttttcgctat agatgaacca
    25861 gtcgaaatgt ttgggttagg acgagtaggt gaattgccgc aagaaatttc tatagaggca
    25921 tttgtagaac aagtaaaaga agcgttccaa ctagatggac tgagaatagt ccagccaaaa
    25981 aatgcaaaat cctctgtgaa gcgcattgcg atttgcggtg gaagcggtga aaaattttat
    26041 ccgcaagcaa tcgcccaaag agcagatgtc tatatcacgg gagatatcta ttatcatacg
    26101 gctcatgata tgcaaagcgc tgggttgatt gcaattgatc ccggtcatta tattgaatcg
    26161 ttatgcaagc aacgatttat cgagaaattt gaatcttgga aacaagaaga aaattgggat
    26221 attaactttt tcgtttctga aacaaataca aacccatttc aatttaatta aaaggagaga
    26281 atactatgta cgaaaatctt ttgccacgtt ttttacgata tgtcaaaaca gaaacacgct
    26341 ccgatgcaac aagcaaaacg actccatcga ctcagacaca agttgctttt gcgcaagaat
    26401 tgaaaaaaga attagaagag ttaggcttag ctgatgtttt ctataatgaa gataacggat
    26461 tcgttatggg aacattacca agcaatgttg atcatgaagt gcgttccatt ggatttattt
    26521 cccacatgga tacagcagat tttaatgcag taggagtcaa tccacaaatc attgaaaact
    26581 atgatggcga gtctacgatc gtactagatg cggaagaacg ctatacattg aatacaaaag
    26641 attttccaaa tttgaaaaat tatgaaggcc acacattgat cacaacagat ggcagtacat
    26701 tactgggagc agatgacaaa tcagggattg cggaaatcat gactgcaatg gagatcttga
    26761 tcaataatcc ttctatccct cacggtacca tcaaagtggc ttttggtcct gatgaagaaa
    26821 tcggtgtcgg agcggataaa ttcgatgttg agcaattcga tgtagatttt gcttatacag
    26881 tcgatggtgg tcctttaggt gagttgcagt atgaaacgtt tagtgctgct caagcaaata
    26941 ttacgatcca agggaaaaat gtacatccag ggacggcaaa agatacaatg atcaatgctc
    27001 tacagttagc aatcgatttt cataatcagc ttccagccga tgaagttcca gaaaaaacgg
    27061 aaggatacga aggtttcttc catttgatgg cattggatgg gaatcctgaa gaagcttcta
    27121 tgagttatat cattcgcgat catgatcgtg aaaaattcga agcacgcaaa gcacaaatca
    27181 cacatatcca agaagagttg aatcagcgat ttgatcaaga acgaatcaaa gtagatatgt
    27241 atgatcaata ttacaacatg aaagaaatca ttgaaaaaga tatgagcatc gtggaattag
    27301 cgaaacaagc aatgatcgag cttggtatct ctccagtgat cgaaccagtt cgtggaggaa
    27361 cagatggttc aaaaatctct tacatgggta ttccgacgcc aaatctattt gccggaggag
    27421 aaaatatgca cggacgattc gaatttgttt ctttacaagt aatggaaaaa gcaacagacg
    27481 tgattgtaaa aattgcagaa ttgaacacaa agagttaaaa tcgaaaaaga acagcttatg
    27541 acaaggcgtt cgtcccaatg atggaatgtc atctgtctaa ggctaaaggg caggacaatg
    27601 gaattggttg tcccgccctc ttttttgaag agtaaatagg aggtaaagaa tggaaaagct
    27661 gctagttttt tataacaaaa gttcagggaa agatgagggt gagaaattag cacgctggtt
    27721 tcaagattat gtaaaagaaa agcagccaga attatatgta gaactagctg aaactggccc
    27781 atcgattgat gatgcggaat tagtcaagaa agcagaagcc atagaagctg atactttagt
    27841 aattataggt ggagacggca cgattcatca tatcgtccaa gcgttccaaa aaacattatc
    27901 tcaataccat gtcggattac ttccaggtgg aactgtcaac aaccttgcac gtgttcttga
    27961 gattcccctc aaaaaagaag aagctgctga cgtgatcctg gaaggcaatg tacggaaaat
    28021 agactatggt caagtcaatg atgaagtcat tatctctaca ctgacaatcg gaatcttagc
    28081 agatacagca gtttggatca gtcaagaaga aaaacagcgt tacggctcgt ggatctttat
    28141 caagaagttt tttaagttat tgctgaagaa gaaaaaatat catttggatt tagaaacaga
    28201 cgaggagcat tggaaaggaa aatcccaact gttgacagtc acgatgtcta actccgtggg
    28261 cggttttaca aattttgacg actcagcgac cctagatgat ggaaaatttc atgtaacgat
    28321 cgtaccttcc ttagatattt tccgatttat tttttatttg cctagaatca tgagaggcaa
    28381 attctatacg atccccggca ttacttattt cacagccaaa cgcatgaaaa tccaagcgca
    28441 ggaaaaatct gtgggcactc gaacggatgg agacacgaca gatgatcttc cagttgaatt
    28501 atctgttgta tcaaaaggat taacagttta ttcaccaaag aaagaagaaa aagcttagaa
    28561 atagtagtta acagaaagtt ggaagaagag taatggatgt attgttacac aatcctttgt
    28621 ggttttacct tttcattgtg aatttttact tattttgtct gatggggtat gataaacatc
    28681 aggctagaac tagcggctgg cgtgttcctg agaataattt gatatttgtc agcctgttag
    28741 gcggtggttt gggaggattg atctctcgaa aagttttcca tcataaaact cggaaaaaga
    28801 agtttactat aggattcgct gcaggtgtac ttgttgattt gattctcatt tattttttcc
    28861 attaaggagc aggagatgtc gtctaaagga acgaaaacta aaatcattct gaatctgctt
    28921 ttgctgattg tgatttttgc cattatttat tatttaatca atcaatcatt tgcagatatc
    28981 tttgccgaat tgctgtctac cagtgttgtt gttttggcag cggctgttat attagggaca
    29041 gtttaccaaa tcgctgaagg gcgctcaatc aaagaaattg ctgaaccttt cgcgccgaat
    29101 tttacaacga aagatgggtt cttgacctct agctatattg cgttttaccg aatcgtttcg
    29161 tttggtacag gaacgctgat ctcggagatt tatttttata acaaaaaagg aatgaagcta
    29221 tcccagggag caggggtgac agcactccat atgattatgt acaaactggc agtgatcact
    29281 tatgcattag ctggcttggt catccaattc tctctttttt attctaatgg tccatcaatg
    29341 atctggttta tcatcgctgg tatccttttg acgatcgtga ttgttgcctt tttattggcg
    29401 atgtccgtca gcttgaatgt tcaaatattt tttgtcagct tatcgaataa gttatttaaa
    29461 agtgagcgtt tgcggaaatg ggtggataca tgcaatcagc agatctactc tttaagggaa
    29521 acggtccaaa ccattatcca agacaggact gcgctattgc ggatttatct atggaatatg
    29581 ttaaaacttg tattttggta tgtacttcct tatttgattc tgattgaaaa ccatcctaaa
    29641 atagattttc tactgaccat ttcgtttatc agctttgcag tagtgctttc aggggttatt
    29701 cctacgcctg ctggtattgg ttcctttgaa tttgtctatt tgttattatt cagaccagtt
    29761 gttggaacag ttgacgcagt ttcttcgctt ttactgtatc gtttcagttc ctttatcttg
    29821 ccatttctct atggatttgt gtatgtgatt gctgaacggc gaagagtgat caaagaagaa
    29881 ataatagaag taaaaaaaga gaataaagaa aatcaagggt aggtctttta taagatctac
    29941 cctttttatc aaatcaaaga aaaacgttat ggataagata attttacgtt tgtttggctc
    30001 tttttttata tacatacaat caattttgtt tttttaagat ttatataagt cgagaaaaaa
    30061 agtcaaaaaa ctatgtgtat attttgacca aaattgacca aagcgatata atagtttttg
    30121 taaagatcaa agaaataagt gatcttacct ttaattagga gggtatacct gtatgaatat
    30181 tgagaaaatg actacgacgc ttcaagaagc gattgctgaa gctcaacaga ttgcggtcac
    30241 tcgaaaacat caagatatcg acattgccca tgtatggaaa atctttttac aatctaatca
    30301 ttttggtcga aatttctata cggatgccgg attggatgtc gaatcttttg aacatgaaat
    30361 tgatcgtcta ctggatgagt atccagttgt ttctggtgga aacgtccaat atggacaaaa
    30421 cctcagccag aatttctttc gattattgaa tgaagcagac caaatacgtg aaagcttcgg
    30481 cgacgagttt ctttcaacgg aagtcgttat tttagcgttg atgaaattaa aaaattatcc
    30541 gttaactgtt tatttgaata aaaacggatt atctgaaaaa gaattgcgta aaaacatcga
    30601 agagatgaga ggaggagaaa gagtgacctc acaaaaccaa gaagaacaat acaaagcatt
    30661 agaaaaatac ggcgttgatt tagtacagca agtcaaaagc ggaaaaatgg atccaatcat
    30721 cggtcgtgat gaagaaatcc gtgatgtcat ccgtattctt tcacgaaaaa caaaaaataa
    30781 tcctgttctg ataggagaac ccggtgtagg gaaaacggca atcgttgaag ggcttgctca
    30841 acgtatcgta cgcaaagatg tgcctgaaaa tttaaaagat aaaacgattt tttcattaga
    30901 tatgggtgca ttgatcgctg gagcaaaatt ccgtggtgaa tttgaagaaa gactaaaagc
    30961 agtcttgaaa gaagtcaaaa aaagtgatgg acgtatcctc ttgttcatcg atgaaatcca
    31021 caatatcgta ggggcaggaa aaacagaagg aagtatggat gccggaaatc tgttgaaacc
    31081 aatgcttgct cgcggtgaat tacatttgat cggtgccaca actttagatg agtaccgtca
    31141 atatatggaa aaagacaaag cgttggaacg tcgctttcaa aaggtattag tcaaagaacc
    31201 aacagtggaa gatacgatca gtattctgcg tggattaaaa gaacgttttg agatccacca
    31261 tggtgttaac atccatgaca acgcattagt agcagcagct actttatctg accgttatat
    31321 tactgatcgg ttcttaccgg acaaagcaat cgatttgatc gatgaagcca gtgcaacgat
    31381 tcgggtagaa atgaactcta tgccaacgga attagatcaa gttactagac gtttgatgca
    31441 gttggaaatc gaagaagctg cactgaaaaa agaatcagat gatgcaagta aaaaacgctt
    31501 gaaaaatttg caagaagaat tagctgaact tcgtgaagaa gcaaatgcga tgaagatgca
    31561 gtgggaaaca gaaaaagaag aagtcaattc tgtttcagca aaacgtgctg aaatcgataa
    31621 agcaaaacac gaattagaag atgcagaaaa taactatgat ttagaacgcg ctgctgtatt
    31681 gcgtcatggt acgatacctc aattagaaaa agaattgaag gaattagaag ctaaagcaaa
    31741 agacagcgag attaagatgg ttcaagagtc cgttaccgaa aatgagattg ctcaagtcgt
    31801 tggcagatta acgggtatcc ccgtaacgaa acttgtagaa ggtgaacgag aaaaactaat
    31861 taaattgaat gaaacacttc ataaacgagt gattggtcaa gatgaagcag ttgatgcggt
    31921 cagtgacgcg gtaattcgtt cacgggctgg attgcaagac ccaaatcgtc ctctaggttc
    31981 tttccttttc ctaggaccaa ctggtgtcgg aaaaaccgaa ttagcgaaag cgttagctga
    32041 gaacttgttt gactcagaag atcatatggt acggatcgat atgagtgaat atatggaaaa
    32101 acatgctgtg tctcgtctag tcggagctcc tccaggctat gtaggttacg aagaaggtgg
    32161 tcaattaacg gaagctgtca gacgaaatcc ttatacaatc gtcttgttgg atgaaatcga
    32221 aaaagcgcat cccgatgtct tcaatatctt gttacaagtc ctagatgacg gacgcttgac
    32281 tgattcaaaa gggcgagtag tagatttcaa aaatacggtt ctgatcatga ccagcaatat
    32341 cggttcacag ttactgctgg aaggcgtgac tgctgatgga acgattccag aagcagtggc
    32401 tgaacaagtc aacacgctat tacgtggaaa cttcaaacca gaattcttga accgaatcga
    32461 cgacacgatt ttatttacac cattgagcct agacaacgtg aaaggaatcg ttgacaaaat
    32521 ggtggcgcaa ttggcacaac gtctggaaca tcaagaaatc ctgctgacaa tcagtgatga
    32581 agccaaaacg tggattgctg aaaatgctta tgaaccagca tatggtgcaa gaccattgaa
    32641 acgatttatc actaaggaag tagaaacacc actagctaaa gaaatcgttg caggacatgt
    32701 tatgcctaaa tcaaaagtaa cgattacttt acttgatgga caacttcatt tcaaaacaga
    32761 agagctagaa gaaatcgtct aatcttccat tgagactata aaagaaaaaa ggggtctgac
    32821 acaactagtg tcagacctcc ttttttcaaa taaacagtgt tccagaagta gtttcttcga
    32881 aaaataagcc gaaatttctt gaagctgccc gcttctgtca caacctcttt tattaggtat
    32941 ttttcacttt ttctaattta taactttcaa agtaacgctc atctttttta ctttgagacc
    33001 atgcaaacaa tcccattaag gcagcaccta tcgtatttgt cagcaagtcg cccatcgtat
    33061 ccatcaatgc tgctcgtccg acaaacagtg tgccatcaga agcagcaaaa cgttgaagat
    33121 tcatgcctaa gaactggtcg cataagaatt cccagaactc ccaaaacacg ccgcaaagtc
    33181 cagcaaacgc aaagccgaat aagaggaaca accaaggact ggtttttgat atctcagcat
    33241 ctttcatcaa gtaaccaatc aatccatagc caagagctgt caggaccatt ggactcaccg
    33301 catgtaagat tttgtcccaa aaagagatga tactgatcaa atgcatccct gtgcctaaaa
    33361 agacagagat gaacaagaaa aaccagtaaa acaaaacgat tgctgctggt attttaagtt
    33421 tgaaaacttt caaaatgact tgtggcatga aaatcaatgc aatccctaat aaagattcca
    33481 cgataaatgt gattccttga gaagtcgaat atttttgtgt gataaattca taaaaattat
    33541 agcagacaac gaatacacca aaagcaagta aagctatttg cacctttttg atttctttaa
    33601 tcactgaaag tccctccatc taaaaatctt tttacttctg aaagcaactc aatttctgaa
    33661 gaaaccactt ttccgttcaa tttgatcaaa cctattgtat ataagttcag gtagtgaaat
    33721 tgattttcag ctgtttcttg tagtgcagcg atttttttcg ggtgggttcc gccttgctgt
    33781 cgagtatcag tatataagcc gatcactggg atgcctttcg cataagccac accgatttcc
    33841 gatgctaccc cggcatcgat cgtcagccca tctagtaaag caatcattaa atcactagcc
    33901 aacacttttt ctgtgtcagc aagagcaatc attcgactgt ctgcataagc agttttgtca
    33961 ttgattgctt cattttcttg aggaagatag atcgaaagct cagaagagag ctctctgatt
    34021 tctttaacta actgttggtt atataacaaa tcacttttag caaacattgg tgcggcaaaa
    34081 taaattttca tctaaacatc atccttttta tcaaaaataa tcgctaaagt aagcttccat
    34141 gtttggaatg atacgctcta gtgacttcag tgtttcttca tctgtttcta accgttcgat
    34201 tcttgccaaa taagaagcac ggcgataatt catcatcaag caagtcaatg tttggatatc
    34261 cagtcgaacg gattttccta taggttcatt taagacacgg acttgtccct gttcatccca
    34321 tgttaaagcg aagacaccat tattccaagg agcgacagga tcttcaatga tgaaatgaaa
    34381 agcatcagct gttccaacaa acggaaaccg ttgaaggaat tcttttacat caacgatccg
    34441 agccataaaa tatggttcga tctgctcttt gatctgacta tcttcaatca aaaatgctaa
    34501 aggctcattt ttataaatat ctccatgtac ccagtagatc atggaaaaat gtgcggaaat
    34561 aaagttccat aagccatttc gagcttcttg atcaagatag aacatttctt ttacgtgaaa
    34621 tatttcttca gctacccagt agaacagaac acctcttggt tcatggttgc tatcgtagta
    34681 gacagcggct gtgcgctcat cttcattttc gaaacgccaa tactcttccc aatgaaaatc
    34741 actgcggaat aaagcaccat ggttttgttg ggcaaatttg tcataaacgg aaaatacgtc
    34801 tggatgatca actggtaatc gctcaaccat accacttact gggatttgtt taggtagctg
    34861 tgtatctctg attttaaagg aaagtttatc agacatgatt tcccatcctt tacgccgata
    34921 gtaggggata ctgtaaggat acaaataaga gatccattgc ttattcttgc gcatattatc
    34981 caatgcaacg ataattagat cttgcatcaa tccatggtta gcatattctg gataagttcc
    35041 aactccagtc acaccaccca ttttataacg tgcaccgtga atattgactt cacaaggata
    35101 gatagcgatt tgagagatca gtttattttc gtggaaccat ccgaacactt tcgatacttc
    35161 caaaatcggt tttttggaac ggataaaagc tcgtttattc tcaaacccgc tttcctcgat
    35221 gtctgcttca gtcacttgga acacatagga aagcaattca ttgaattgat cgatatgttc
    35281 ttgagtgacc ggttttaatt caagattttc tctaaaatct ttttgatcca ttttttcatc
    35341 tccatttatt agcttgataa cagtatagca aaattctaca aaaaaaactt ctatagctga
    35401 ctaattaaaa actctttaaa ttactgcttt tcgttggtat aatggaaagt gctgattata
    35461 tgaaaagagg agacgctatg gatataaaga aaatgaaaga acgccaagag cagattcgta
    35521 atttttcgat catcgctcat atcgaccatg ggaaatctac attggccgat cgtattttag
    35581 aaatgacgca cactgtgacg tctcgcgaaa tgcaagatca gctgttagac tcgatggatt
    35641 tggaacgtga acgtggaatc acgattaaat taaatgctgt agaactcaat tacacagcga
    35701 aaaatggaga aacatatatc tttcacttga ttgacacccc agggcacgtc gacttcacct
    35761 atgaagtctc tcgaagcctt gctgcctgtg agggtgccgt tttagtggtc gatgcagcac
    35821 aaggaatcga agcacaaaca ttggccaatg tttatttagc gttagacaat gatttggaga
    35881 tcctgccagt catcaataaa atcgacctac ccgcagcaga tcccgaacgt gtccgtcaag
    35941 aaatcgaaga cgtaatcgga atcgatgcta gtgaagctgt attagccagt gccaaatcag
    36001 ggattgggat tgaagatatt ctcgagcaga tcgtggaata tgttcctgca ccaagtggag
    36061 acttagatgc accgctaaaa gctttgatct ttgactctat ttatgatagt taccgcggtg
    36121 ttgttctaaa tgtccggatc acggacggta tggtcaaacc cggagacaaa atcaaactga
    36181 tgagtaatgg caaaacattt gatgttacag aggtcggggt cttctcgcca aaagcagttg
    36241 ctagagactt tttgatggtc ggtgatgtcg gttatatcac agcaagtatc aaaactgtcc
    36301 aagatacacg agtaggggat acagtaacgt tagcagacaa tcctgccgct gaagctttac
    36361 caggttaccg aaaaatgaat ccgatggttt attgtggtct ttatccaatt gatacttctc
    36421 gttacaacga tttgcgggaa gctttagaaa aactgcaatt gaatgatgct gctttacaat
    36481 tcgaaccaga aacctctcaa gcattaggtt ttgggttccg ctgtggattc ctcggacttc
    36541 ttcatatgga tgtcgtccaa gaacgcttgg aacgggaatt caatctagaa ttgatcacga
    36601 ctgcaccatc cgttatttac catgttaata aaacggatgg tacgactgct acagtagata
    36661 acccggcaga ttttcctgaa cctgtaacga ttcaggatgt agaagaacca ttcgtcaaag
    36721 cacagatcat ggtcccaaat gattttgtcg gtgcagtaat ggaactttct caacgaaaac
    36781 ggggagaatt cattacgatg gattatttag atgattaccg agtaaatgtc gtctataata
    36841 ttccgctttc tgaaattgtc tttgactttt tcgacaaatt gaaatcaagc acgaaaggct
    36901 atgcctcatt ggattatgaa atgtctggtt atcaaaaaag caagttagtg aagatggaca
    36961 tcttgctgaa tggagaaaaa gtcgatgcat taagctttat cgttcaccga gactttgcct
    37021 atgaacgtgg aaaagcaatc gttgaaaaac tgaaaaaact aatcccacgc caacaatttg
    37081 aggtgccaat ccaagcagct attggacaaa aaatcgttgc ccgttcagac atcaaagcct
    37141 tgcgtaaaaa cgtcttggct aaatgttatg gtggagatgt ttctcgtaaa cgtaaactct
    37201 tagagaaaca aaaagaaggg aagaagcgga tgaaacaaat cggctccgtc gaagttccgc
    37261 aagaagcctt catggccgtt ctgaaaatgg atgagcaaga tagtgtgaaa taacttataa
    37321 tcaatcgaaa aacctattaa ataaaccttt ttgagtagat gcaacactga ggattatctg
    37381 tataaaagat tttaaactgt catttggtta tcacggcgat actaaaatta acaaaattgg
    37441 aatatttttc ttgaagattt ttagctgtca gtatatgatg aaagcatctc ataggaggtg
    37501 cttttttcat ggttgataat tcaagtggaa gggttcttaa atctctaaga aaagagaaaa
    37561 aactatctca aaaaaagtta gctgatttag caggtatttc acaaagtact ttagttaaat
    37621 atgaaaaagg gtcaagaaaa atccctaaag acgtagataa tactctaagc aagattctga
    37681 atatagaaac tctaataaaa gatgaggaag ataagattga gattctcatc ggtaaactaa
    37741 ttgcatacag agatatgaat aagttactta acaaagagtt agctgataat ataggaatta
    37801 gtgaagtatt actcagttat gtgttaaata gaaagagaaa tccgtcaaaa gaaatgcaaa
    37861 aaaaaatcga tatttttcta ttaagtaatg aaaaagaaat tttgaaggag attaatcgtg
    37921 atagtgagat atttagttta tcaaaagacg ataaaattgt tatgggaaaa agaatacgag
    37981 aagtccgaaa gaaccgggaa gagacgcttg aaaaatttgg taaaaacttt accatctata
    38041 caggcaaaaa tgttataagt agatgggaaa aaggtattaa tattccagat attgaaaaac
    38101 taatgaatat tgcctatctt ggtaaggtca ctgttcctta ccttatgtac ggagaagatt
    38161 ataagaatat acttccaaaa gatgaaagag ttagcgattt taaaaagata aattctttta
    38221 gtatgggatt aaggatgagg aaaattagga aagattatta tctagaaagg gaggagtttg
    38281 gaaagctttt ttctccttca atttcaaaat ggtccataga taggtatgag aatggtagag
    38341 atatacccaa tactaatcga attatccaat atgcttatat aggaaatctt tctttagaat
    38401 ttttgattta cgggatataa aaatagttta cgtcgatact tattaagtta tcgacgtttt
    38461 ttattataga aaaaatttat aaaaggaggt ttcttgatat gataagaaac aaaaagggtt
    38521 tgttgaaagt tgctttactg gcagcttttt tctaagtaac tttttgacaa gctcaatggc
    38581 aacacaagcg gaagaagtaa tacaagaact aactgttatg aataacagct agataaaatg
    38641 ggctatgtat gttatccaac aatttagtaa tcactagaag cctagcgaaa gttcgcttga
    38701 taagttatag atgagtattg atttgtagtg tttccaaatc aaaagggtac agagccacat
    38761 gaaatatgtt gtcctctgca gcgtcaggat gggaatccat accatggaga ttcggagatc
    38821 tgtgagggaa tacttacgtg gttaatatgt cagttgtgtt ggacgtagta atggtcatag
    38881 tgggtagatt gactgaattc ttatatgaga aaatgaaacc aacaagaaga tagggttatt
    38941 tatacagaat tggggatctt ttctggaaaa gtaatcggat aatgcgagat tcgtacggtc
    39001 ttttttaaat ttctctaaaa aaaacggcat cgaatgggtt gggtgattga taagcaatga
    39061 atcatagact gatatagaat aaaaaatgat tctatttatc tttttacatc tatcgttttt
    39121 tcaagtagcc gcaaattata tcagcattca acaatttttg tatacaagtt ctagtaaaag
    39181 gatctgaaga aaaagttggt tgttgtgaag ataaaaaaag aatcaggttt tactgagttt
    39241 ttcttcaaaa tttgtgttat taggttagga caaagataag aagaagtatg aagatttaaa
    39301 aaaggaatga ggaaaagata gtaatttccg attattggag tttttttatt tataaagaag
    39361 aagcgaataa gttgtgattc ctagatttcg cgcgtgatta attaatgagc aaaaaatgcg
    39421 gaaagtttta gtgtttaata cacgaggaca aagtttctta ggtattgaag gcacacgtgt
    39481 aacggttgat gggtggtgtg attgtttcga ggttggaaaa catgttgaac ttatggcatt
    39541 tactgagcta aaagataaga acaacgtgga atttttcgag agtgatattg gttgggacga
    39601 tcatgaagaa gtttatggaa aatttatttt gcggaaggga aattcttata cgaatgggga
    39661 agcataagta gcgatttgtt agagatcatt gataggattg aaattgtcgg gaatatttat
    39721 gagaatctag agttatttga ggtgatagaa tgagtgatgg ttttgaacaa gtatcaataa
    39781 ttaaaaatca agttcgggag attcttagaa aaaaaggcta tttggtagat agttattttg
    39841 aaggagatta cgaaacatgg gtaggtgtct atgctcgtcc agagaataaa cctacctatt
    39901 tagatcctac gacgtcagaa gatgcctatt tacaaaatag gtatcgagta gatggtttta
    39961 agcaagactt tgcggagtgg tttgaatggg aaattgaaaa cggagaagta aaagaagaat
    40021 aagagtactt gttgtatcaa aataagccag tagaacaacc aaaccagtag ctagtaatga
    40081 caataatact gaggaatcgg atcattatgg tatgattgat atttctcttc cagaagatct
    40141 taagccagat cctgtaacaa ttggcaatag tgtcatgatt tttgattcat tagatgaagc
    40201 tatgtcatat ggttggaaag aaattaagga tgaaggtagt aattggtatg gcatgagatt
    40261 tggtgatggt gaagttgcca aaaaagctgg tattccaatt gggaaatgga cagttgattt
    40321 ccacgaagca ggttcttttt ttgttctgta attatagtgg gagggtaaaa tcattgatga
    40381 aatgtgttca gataagagcg ccagataaaa atagaagaga cagttcacgt cgttatcact
    40441 tttggtaacg gcgttttgtt ttattatata aaattctcag gaaaaggagg aattcttgtg
    40501 tatgcaaaaa cgaaaatgaa ttggttgaaa gcactcgcat tggtggcttt ttctcaattc
    40561 tgtttcttgg tgggaactgc tcaagcacat gcagaagaaa cgacggctat acaacctacg
    40621 atggaagcca ttgcccaagt cgcttctgaa gtgacagaag tccaaccagc agaagccggg
    40681 gtcccagctg gtgaagccac tgttgaacag gcccctgtga cagaagcgcc aacggtcaat
    40741 tccaaaacga ctctcgatca gacgattggg gaaacacaac acccagggaa tagtcctggt
    40801 acttcacaac cagttgaaga aactgcctca attacaaccc ctgcttcaga aacgtccact
    40861 ctagaaggag aaaaagctgc cgaagcaacg ccttctgtgc agccagagtc ttctgagaaa
    40921 caagcggaag aaaagaaacg aatagaagaa acagtggaac aaccagcggc tactgctgac
    40981 aaagaaggaa aaaatgagga aaacgttgaa aaaacaatct cttcaaccgc agctagtcaa
    41041 aatcaaccaa cgaaaccaaa caaagaatct acggctagta ttgaaaatca aaagaaaaaa
    41101 gagcagaaca ttccaaaaac aggtacacct acactgttag taacagtacc aaccaatcgt
    41161 tcggttcgtg ttcgccgtgc aaccaatgca gctgttttag gtggtcggaa aattgattgg
    41221 ggcgaaaata ttggtgttac ttctagtccg aacaatctaa acttcacttt aaattatatg
    41281 gttcgttttg agattaatgg acaagtggcg ttttgtattg agcctggtgt ctcagccgca
    41341 gaaggtcaag tttatacagc tactgctttg aaaaactatt tgaaaaattc aaatgtacgg
    41401 aaaaaaacaa gcttgatctc ttatttagga tatgtcacaa ataaagacaa atccgatgaa
    41461 caatacattg cggcacaatt tatgatttgg gaagctttgg gcacaaaaat cccaatcaaa
    41521 aatatccgtg ttgattatga tcgtcgaaaa gcagaaattc aacgtttgat taataacttt
    41581 ggtgaaaaag caacttttaa taatcaaaca catacggtaa aaattggcga aacgttgaaa
    41641 gtaaagaata aaaatgattt tttgaaatat gtgaaagaca ttcaaaaacc aaaaggtgtc
    41701 aatgtatcta ttcacggcaa tgaagtatgg attacagtca caaaagaggc accggataaa
    41761 gcaaaaattc aattgaataa aattaaagtt caaggtgcgc cattagccta tcgtcgtcca
    41821 ggagctcaaa caattggtgt gttacatcca acagaaccta atgcgtctgt tattaattta
    41881 caaattctaa aacaaggaaa cattcgtgtg tttaaagaag atgcggaaac tggtgtgaaa
    41941 gcacaaggtg cagcttcttt agaaaatgcg gtctatggtt tgtatcaagc agatgggaaa
    42001 aagattaaag aaattacctt gaaaaaaatt aacggcaaag tacaggcaga aattaaagat
    42061 cttgatcctg gcaaatatgt tttaaaagaa atcaaagcgc caaaaggtta tgtgctttct
    42121 gatgaagcaa ttgaaattgt tgttgaacct ggaaaaactg tcaatgcgac cgcaaaagat
    42181 caagtgatca aaggaaccat tgatttagtg aaagttgcta ataaagattt agtcgattca
    42241 acgaatccag ataacaaacc aaaattagct ggcattgaaa tcactttgac gtcaaaaaca
    42301 actggcaaag tggtgaaaac cgttgtgaca gacaatgatg gttatgctac ttttggtaaa
    42361 aataccgttg tctttgatac gtacattctt tctgaaacca aaggaaaaga agggtataaa
    42421 ctatttgaac cacttgaagt cacaatttct gaaaatgggc aaactttcca ttatgtttta
    42481 gaagataaag taattgaaca acgtcttaaa gtggtgaaag tggatcaaga aacaggaaaa
    42541 gtgattccac ttgctaatac agaattcaag atttttgata ctttagccaa tcgttatgtc
    42601 acaatggaga ttccaaatga tacagaaaca acggatatct ttaaaaccaa tgaaaaaggc
    42661 tttttcctaa ctaacggtac acttacttat ggaaaagatc gctatcgttt ggaagaaatc
    42721 aaagcccccg taaattacgt tttgaatcaa aacccattag tgttctcgat taccaatgat
    42781 acagaaccga tcaaagtgat ccattttgcg aaccgtcaag cacggaaaag tgtaaaatta
    42841 ttcaagtatg aagaatggaa caagaagaaa acgccgctcg caggtgtgaa ctttaccttg
    42901 tataacggta aaaatcaagc aattggtgaa tataccactg atcaaaacgg ggaaatttta
    42961 gtgaaaggtc tagttgccga tgactattac ttcttggaaa atgccccatt agaaagtttc
    43021 caaccaaata cagataaaca agcattcacg gttgcattca aacaaaatgc gccagatgat
    43081 ggggaaacgt tagtggtttc tgtacgtaat tacttgattc caccaaaact agaaacacaa
    43141 gcgtcaaata aagccgatgg tgaaaaagtg gttgatccat tagaaacgat cgtcattaaa
    43201 gacagtgttt cttataccaa cttgtttgtc ggaaaagaat acaccatttc aggagtattg
    43261 atgaacaagg caacgaatgc gccaatttta caagatggca aagaaatcac aagtaccttg
    43321 gcctttattg cagacaaagt caatggcaca aaagaccttg aatttgtggt taatgctagt
    43381 gttctgaaag gtcaaacgat cgttgttttt gaaaaacttt tccgtgatgg cattgaagtt
    43441 gcagcacatg cggatatcaa cgatgaagcc caaagtgtga aagtgaataa tccaaccgtt
    43501 cataccacgg ctgtctttga agacggattg acagtcgcag acccaacagg attgtttacc
    43561 ttaaacgata ccgttgaaat taccgagatt cttccaggaa aagcatactt ggtcaaaggg
    43621 atcttaatgg ataaagcaac cggtgaaccc ttgcttgtgc atggaaatac ggtagaaacg
    43681 agtgttggct ttattgccaa agcagaaaat gaaacggtga tcgtaccatt tacctttgat
    43741 ttgacaggta taaatggtcg tgaaatcgtc gtgtttgaaa gtctttttag aaatgatgaa
    43801 gaactagcaa accataaaga catcaacgac aaagggcaaa cggtacgagt aactaaccca
    43861 agtgtacgta caacggctac acataaaccc actggcttga aagaggttaa tccattaacg
    43921 aaagtcacca ttacagacgc tgtgaaatac cacgatttgg tcgttggaaa aacatacaca
    43981 gtcaacggtg ttctaatgga caaagtaacg gaaaaaccag tgctagtaga tggaaaagaa
    44041 gtcacaagta ccttaacgtt tgtggcagaa acaacggatg gtacagtcaa tctagacttt
    44101 attctagacg cacgtgcgtt gcgaggcaaa gaagtcgtgg tctttgaaga tctgtttaga
    44161 gaaggcgtat tattggcttc tcacgcagat attaccgaca atgaccaaac catcaaaatc
    44221 agtaatccaa ccattacgac gaaagcgacg aataaaaatg gcggaaaaga gatcttggcc
    44281 ttgccaaatc aaactgtgct tgaatgggtc aaagtagacg gtcttgcgat tggtcaaatc
    44341 tatcgcttaa ttgttcaagg ctacttaaca ccagacggca caccatttga aggcactcat
    44401 gcggaaaaag tctttgcggc cgataaagaa atgatggaat tcttatttga attcttagtg
    44461 gacggtcgca atcttgcagg aaaaggcttg agttttgcgg aatggctcca agcgaaaacg
    44521 gaagatgaga aagaaacgtt tgaagaagtg gcgaaacaca atgtagatct aacaaacaaa
    44581 gatcaaacgg ttcaaattgt ggaagcgcca aaaccaccgc aaccagtgaa accagaacaa
    44641 cgggttaaac cagctactgt tgtaccaatg ttgccaaaaa caggtgaaca aacaagtagt
    44701 atacttatgg ttcttggatt agtagtatta gtcattgctt taggtataac actttataaa
    44761 gtacgtaaag ccaaagatta gtcatgagga acaagcggtt aggattttta accgcttgtt
    44821 tgattgtaat gtagaaatat ttagaattta ttttggtttt taagaggagg aaaaacttta
    44881 tggcagaaaa agcattgcgt atgaagaaag agtttttagg tcaagtattt ttattgggtt
    44941 cacgggaacc taatccacca aaggaaatta atggtgtgtc tgggcgatat gttcaattac
    45001 aagcagagtg ttcgcgagaa gaacagcctg ctttttttgt ttatctcaca gctgaagagg
    45061 ataaaagatt acgattacca gaaaaagagg tgcgtatttc atttgaagaa gcttattttg
    45121 tttttcgtaa agaacgagtg aatagtcgtg aaacaaatga tatgggacaa acagagcgtc
    45181 gaacaactgg ctatcaagat gttggtgtct ttttaactgg tgtaaaaatt ggggaggttt
    45241 tgtaagatgg gattaaaatt tccaaataat tatgacatta cagaagcatt agatgaaact
    45301 tgtttaggag ccgtacgctt tggcgcatta gaagagtctg atcgtgtatt tgataatgaa
    45361 acaggcgatt atacgggaga aattcgatca cagcgagcac aattagtttt tggaaatcca
    45421 gatgaaccaa agattgcacg acgagttaaa tttccagcag atgtagattt gactggtttt
    45481 aaacgacgtg atcctgtgaa attaattgat ccagaatatc atgtggtata tattccagca
    45541 caagatggca atcgtgaata ttcagattta gtgatttatg cgaaaggatt agagaaagta
    45601 gaagctccaa caaataatcc taatatgaaa caatcacaag aaaaaggttt agatcaaaaa
    45661 caaccaagtg ttgattctaa ggaaaaagag aaaaaataag gtttttttga aagaatagga
    45721 aggagtcttc ctattctttt tatctaagat agaaggaagg ggagattttg aattcagata
    45781 aaaccaatca aatactttga gagattgtgc ttgttatcaa aatatccaac aattgattga
    45841 gtattttgga gacaatccaa cgatttttgt acaaacaaat gcatatattt gtgcggttat
    45901 tgggcagtgt atgtttttac gtgatcttgt tcgtttttca ttaccgtacc agtggaaaca
    45961 agatcatcag gaagtgattt ggattcgtat agtacgaata tgtaatatgt gtgcacaccc
    46021 ttataaaaat cgattaaatc tttttcgatt atgttttttt actaaatgtg atattcctgt
    46081 attagttgct ttttgtcaaa agtttttagc acaggaaaag taaaaacaga aaggaggaat
    46141 gtgacaattg aaagctcatt ttttcaagta tcgtggtcgg cgaattcgct atttcaatcg
    46201 ttatttatgg ttttgctatt atggtttctt tttgtttcca tttttgattc tgttaggttg
    46261 gtttagttgg aaagtatttt atccacgttt aagtacgttt tcgaattggc aaatgtatct
    46321 tattcctgga ataagtattg tgttgtattt gttgctggtt agtgggttaa ttttagggtt
    46381 gatgcattgg tcgcgactaa aagaaggata ttttgcctct gtttattggc gtcagttact
    46441 tgttcgaatg ttaattgaca atcggtttgt ttatacaaag aaacaagtta gtgagaaaca
    46501 aacaagggaa aaaatgagat taccaaaagt ttatttttat caaaagaaac aagaattagt
    46561 ggtttctttt cctttagatg gtgggcgttt tcacgaacga tttttaaaaa tgggtcattt
    46621 gttatctgaa gtatttttgc gtgatttaat tcgtgaagaa cgagaaaaag cccgaatcaa
    46681 ttatgtattc ctttcagatt ctggtcggat tcctattgat caatgtatag cagaaaatgg
    46741 acgtattcat ttaatgaaaa ctttggattg ggtatatgat caatcaccaa atatgttgat
    46801 tgctggtgca atcggtggtg ggaaaaccta tttattgtat tctttaattc aagcttgttt
    46861 aagtgttggg acagtagata tttgcgatgg aaaagcggca gatttagcag cattaggtga
    46921 cgtcgatgta ttcaaaggac atgtgtttta caaaataaat gaagaaatga ttatttgttt
    46981 acgaaatgca ttaaaagaaa tgaatcaacg gtatgcctat atgaaaacca tgaaagatcc
    47041 taaatatgaa ccagggaaaa attatgctta ttatgggtta ccaccgcatt ttatcttttt
    47101 tgatgaatgg gcagcttttt atggttcgtt ggatatggaa gcgagaaata gagtcgataa
    47161 attgattcag ccattagtat taaagggtcg tcaagcaggg atgtacatta ttttagcaat
    47221 gcaacgacca gatgcagaat attttccaag tggtgttcgg gataatttaa cattccgtat
    47281 ttctgttggt cgattgtctc ccattggtta tttaatgact tttggagaag attttaaaaa
    47341 taagccattt tacaacaaag atcaacctgg tcgtggatat gcttttccag gatttgatgt
    47401 acctgtagag ttttttgcgc catttgttta caaagattat cactttatca aagaatttga
    47461 gaaatttact gaaatgattg tctatgattt tacagtaaaa gaggatagtc aagatcaaac
    47521 gatgggagat gataatcagt tgttacaaga acaactagag cgccaaatgt atttagcaaa
    47581 agatccaaaa ggaacaagtt tttctgatgc gcatattttg taatcaacta aggaggtgaa
    47641 gttaaatggc agagagttgt tattatagag tagctacagc aatacgaatg attaatcctt
    47701 ctctttcttc cagaacgttt tatgattggt tgaatcgtat tgaacaagta actgattacc
    47761 gctttttgcg caaagaacgt gtttttacag gaaaagttat taatcaagta ttgctaacga
    47821 aaaaagatat agaacgattg actcgattgt atcattatcg agtagattta gaagaggatt
    47881 taactttgtc aatttatcgc gttttttctc ctgaaaaata tagtgaaata acaaaattag
    47941 atcatttaat tttgtagtta ttatctatcg ggagggctct agacgtagtc tggtagccgc
    48001 actctgcctg gcggtgcgca ttaaggctgc gaagcatggg cctataatgc gcactcgcta
    48061 gcagcgtgtg cagctcacaa agaggtcgtg cgcttgcgca cggcctcttt gtgtaaagcc
    48121 ccccgatata taatatgggg ggcagaaata acataacaca tagaaaaaag ttttataaac
    48181 ccttttgtat caagtgatta tctaaggtca gcagttgtac tacttttccc aaaaaacgag
    48241 ggggactagt tgatatatcg gtgtttctaa agggttaaaa aaatcgattt tgttatttta
    48301 taaaaggagc gagaaaatga caccgattga gctaaaaagc tacagaaaga agttaagtgc
    48361 aattcataat ataagtggag ataaaaagcc gctatcacaa gcaagatttg cgcaattgat
    48421 gggctgctca aaagattaca ttagaaagat ggaacaaggg aaaagaaaaa tcccagagga
    48481 ttttgaacaa cgaatcaagg aacgctttcg attgtatatg aaaagcggaa tagatcttcg
    48541 agcaatgatt gattatttgc gtctttcctt ttttgggaca gactacaaaa cagtgattca
    48601 acgtgtatta cgaatggatc tgacagaatt tgaaacacgt ccaacgggct tgtatctttt
    48661 tgactgtctt tctgttcgtg ggaatatctg ggtgttctgg cattcaaaac gaactgtaac
    48721 agaaaatgta cttgtgcaat tttcaggaca aggttgccgg gaatatgagg aagtcatgcg
    48781 tgaacaacaa acagattggc gaacatggct atattccatt tggaaaaatc aaggaaagtt
    48841 ggaagctggt tatacacgtg tgcaatgtag ccgattcgat attgcgttag atgaactttg
    48901 gggattggaa acggaagaac atttcgatct ctttgaatta ctagaaaaac aaaaagcagg
    48961 tttattagaa atggacttta agagattcca gaatattggc ggttgttttt tagatgatgg
    49021 ctattttcga agtgaaggat tatctttgta ttttggatcg cgaaagagtc ctctcttttt
    49081 taatttttat gagaaacgtt ttgaaattgc gaaccgagaa aaaatttcag aattagaagc
    49141 attaacgaaa tatggtattt ataatcgata tgagttacgg ttagccaatg agaaagcaac
    49201 acaagcagta gaagcattta ttttaggtga ttcaccgaaa cgtttaggag agattggcgt
    49261 tggtttaatt aatgcaaaac ttattgtttg ggatagactg gaaagtgatg cacggattgt
    49321 tgataaacga tgggcaaaat tatttcgttc gatggaagaa ttggatttta cagtaaagcc
    49381 agaaaaattc tcattagatc gttcagaacg ctggtttttg aaacaagtag ctccgacatt
    49441 aaagttgtta caagaagcag atcgtatttt aggaagagac tttgtacaag aagggattga
    49501 taatgccgaa ctaaatgacg agcaacaaaa ctatctagaa tggatgggaa cgtcaggagg
    49561 gaaaattgca tgaatgcata tacgacaaca gaatggctgc gcctgttgga tttaaaggct
    49621 gcaatcgaag cattaaatga aaagatggtt gatttgtctt attttcgttt tcgagttcct
    49681 tatattgaac aagcggtaaa agctggacgt tatcaggaaa aagagaactg gcaagaaatt
    49741 gcacgcttat tagaagttcg taagggatac gaacaagaac tcgaagagtt ggaatttagc
    49801 cgaaggaaag gaaggctaga attcattcgc ttttatcgat tttcattgcc aatacctgcg
    49861 attttagcgg ttaaaaaagg ctgcgataaa atgaagatat atgaaaactg tgtggcagca
    49921 cttagcagcg aaaaacctct tatggaagaa atttcattgg tgactgtcac ttgggaaatg
    49981 agtcgccagc caacagaaga acagacatat ctttctttag aagagattga gatagaatta
    50041 gaggaaattg gtcgctatgc cacttgttct acctattgtg gatcggtcat tagtattgct
    50101 ggtgtgattg tctaaatgaa aaaaattatg atttatgttg gtgcgtattg gtcacgtgat
    50161 ccaacagtat tagaaaatcc agaggcggta cactattgct tgcgccaatt attctatctg
    50221 tataaagaac gattagaatc gttgattcgt cagttaccat atactgacag acgtttagat
    50281 gaacttcttc taaggtatcc agcaatgtat aaacgacgaa aaaatagatt gttaccagaa
    50341 gagtatccca ttgaaaaaca tgaacttgaa gggcgatttg tggcttattt ctatgatgat
    50401 gttcgtatgc gcttagtaga acaacgaatg gaaatagaaa atgatcgcta ctattttatc
    50461 aattattgta agaagcgtaa gtatcaagta actgatgatt tttatgattg tattttacaa
    50521 gatggggaag tgattctatc caaaattgct ccaagttttc gtgaacttgt gccgattgat
    50581 tttttgaaaa aatgtcattt gcggattctt ccgtaaaaaa gagctataaa aggaaagaaa
    50641 agtagaagcc aaatcggttt ctgctttttt atttttttta ggaaggagaa gatggtatgt
    50701 cactggaatt tttaggagag ccaattgaaa aggaagagtt cgtttcgcat tatatggatt
    50761 tgtttattca ggtgataaaa aactttgtgc gattgatgaa ttttatccac gcgaacaaga
    50821 ctatctagcc gctgaacgaa taaaaatcag tttattatgg gaaaaattca cagatgaaaa
    50881 taaccgaccg ccagattatc gctatttatc agattgttta acgaataatg tgctttctaa
    50941 agaataccta tttcacaaac gtgagaaaaa tcaaatgtcg gttgaacatt tttgtgattg
    51001 ttatattgca ttattgcgta gtcaacagtt attaacagta gaacgatatg gtgcagagtt
    51061 tggttatgtc ttgcaagctg agcgtcatag agctacaaga tgctatgaaa aagaagatca
    51121 agtgattgat ggctatgaag aattatgtat tcgaaacaat cgctatttgc aacaacgttt
    51181 atatgaacaa ttgatgatgg atttttcaaa tctctataga gggtatgaat gttcgatgat
    51241 tcaagaaagg tagaagaaaa gggggaattt ttatgtggcg attattattt ttggtggtat
    51301 gtggtatggc attgttctat gtgtatcgtg tctatctcta tccacgaatg gaacgatgga
    51361 caagttggta tagagatcct ttaaaacaac gaaaaggatt gaagcggtat cgttatgaat
    51421 tacttgttga attttcggat gggcgacaag cacagtatta tttttttgca aactatccaa
    51481 aatacaacac cagtgaacta ttgattcaat tattcaaaaa taatccatat gttgggttag
    51541 aagaaaacgg acgggagttc ttttatcgtt cagataatat tagtgcggcg tctatgaaca
    51601 aaacacgaat tgcataaaaa attggaggaa taatcatgaa aaaaacaaca ttagcagtcg
    51661 taacaacact aggaatttca ttattttcgt ttcaattagg gagttcattg gtttatgcag
    51721 atgaaactgt agaaacacct aagtcagaaa ttgtagaaac attgccaggg gaaggattac
    51781 cagatgttga gacagagcta aatggaggaa caacggaaaa accagcagaa cctgaacaat
    51841 tgcagtcaga gaaacctgaa aaagagccag tgacaccaga accagagttt cctgaatcaa
    51901 ctttaccgac tgattcgatc gaagaagaag gtgtgcatag ttcaacaaca acggattcaa
    51961 gtatagtcac aactccggaa caatcaggaa caaccagttc ttctgaaaag aatactgatt
    52021 cgtcaagtac ggaacagcca acgatgtcgt ctaccagtga agaacaaaaa gaagaaccga
    52081 aacaacctgt tgaaaaatca gaagcaattc caaaagaaaa taaacctatt gttccagcaa
    52141 aagaagtcac agtatcggta accccaagcg gtgaaattac gacgaatact tctcaaggaa
    52201 tgagtgtacc gattgtcact agtaatgtag aggaattgac ccatattcca acaccaacca
    52261 ccccattaaa agtagcaggt gatcaaacaa ttgttggtgt aaaagatggt attccattaa
    52321 tccaagatag tgaagggaat ttggtaaagg atttgtcgat tcctgtaaag aaacttccaa
    52381 gtggcaatat tgaaatcaaa acagcagatg gaaaaacaaa agtcttacca aagactggtg
    52441 aagaaataca tgttgcactt tctgttctag gaggagtgtt gacaagtatt gctggatttg
    52501 ttggatataa aagaaagaga aaaggaatag aaaatgaggg taattgaatg gactattttt
    52561 cagaaaaaca agcttatcgt tgtcaatcgt acgtactagt aaaaacatac tagaaggagt
    52621 agcttatgaa aaaacgacaa ctacggcgga aagaaaaagt ttatacagag aaagtaccga
    52681 agcaaaagaa aatccattca aaaaaagcag tgaaatggat ttggtgtttg gttttatttc
    52741 tttgtttttc agggggcttg gcattttttc aatccatgaa aacgaaggtt caagtgtcgt
    52801 ttttgaaaga tgaaatccaa caattacaaa agaaagaagt acaaagtcgg tcagaattaa
    52861 cgtacacacc tgaaattgaa tcctttatga atcgttttgt tgctttgtat atgaatacat
    52921 tcgataatca tgaacaagaa caacaacgtc aagaaacatt aaaaaaagag ttttatgcac
    52981 aaaactttat tcaagaacca ggaagtgttt caggtgttcg aaaattaact gctgcttcgt
    53041 ttattggatt aaaagaaatt gaaaccattc caactgctag ttataaagta acttatgata
    53101 ttcgctcgat gaatccagag agtaagaaag aaacaagtgt gacaatcaca caagtactca
    53161 atcttccctt ttctatttca aacaagggct gtcgtattat tgcttatcct tattttacgg
    53221 cactaccaaa gaatacagaa aaacaacgaa tgattactta tgatgacaca ttatacgaaa
    53281 cggttgatgc gtcggaagcg aaagatgttg aaggatttgt ggaagatttc ttagataagt
    53341 atgcgaaaaa caatgcaaaa gatatgtcat acatgatgaa agaacctgaa ggactcaatc
    53401 aaacagctaa agttgatcaa gtagagatga aagtattgcg ttataaagaa acgaaaaaac
    53461 cttttattgt aaaagcaacg gtcgtctttc gagatggtga tacgtcactt ttacaaaaag
    53521 aacaaatgac gattgtcatt acgaaaaaaa caaatcagta ttatgttgaa aaattaacac
    53581 ataattggtt ggataggaaa ggagaagaac ggaaatgaca ctgaagggaa ttgtagattt
    53641 tatattaacg caaggtggct attttttaat aatggtggtt gtttatctgg gattgcgaaa
    53701 ttttatgaaa tcaaaagtag gaaatgcatt aatatctatc gcagcaggtg ccgcagttta
    53761 tttcttctta aatgatccaa cacgtgtatt tgatgcgttt ggtaaaatca ttgaaaaatt
    53821 atttatggat taggaggata gtgaagtgga ttgggaacct tatgattatt cgattgttta
    53881 tgcggaacct tatcgcgtac aacagattac aagcaattgg cgtcttccat ttccagttgc
    53941 attagtcaat gcagcaactt ttttgttggt cgtaatcttt gaatatttat ttttacgttc
    54001 atttattggt tgggtcattt ctttttttcc accagcagga gttgtttttt atataggact
    54061 cccctattta tgtgtgttag cactggcaaa actaaaacct gatggaaaaa aagtgcattt
    54121 ttatttatgg gattggtttt gttatatgat tcgtatgatg aatggtgatt gtgtgtatta
    54181 taactgggac aaggtatcaa acaatgcatt ggaagaatta ctttttgtga ataagtatgt
    54241 ttatttagag gattagaaaa agggacaatc aactggttgt tcctttttta ttgggaggtg
    54301 aaattcattg gtgcttgtaa aagaaccagt aaaagcattc aagaaaaatt gtttgttaaa
    54361 tggagaggat tgtatctggg cctactatca tgtttttcca cgaacaattt caaaaaacac
    54421 accaaaaaag acacaggaaa taaaagaaat gatggctcgt ttactgttgc ttttagcaga
    54481 ttatgaagat gtggagttgg tgatgtatcc acgaacaatg aatttacgga cgaagtttga
    54541 cacattagga aagggatttt ctcccgatag tcaagcaatt ggtgcgtatt atgcaaaaga
    54601 aacagtgaca ttgttggaac gagaaggtca agtctatcgt ccttcattta ttttaggaat
    54661 caaagtcaaa gaaaaagcaa cggtggagag ttttcatgat gtaataaatc taatctggtt
    54721 agatttagca ggaagtttag cacgcttatt tggattagaa gtagatgatt tggaaacttt
    54781 gttttctgaa agtgatcctt atgtaaaaga agtgacacaa ttactttata gcttttctat
    54841 tcgtccctta gtaaaaaaag agctgttgta tctcaatcgt ctgcaatata ttcgaggaat
    54901 ggctcacaaa gtagaagaac aaaataattg ggaagtactg aaagacaatc aaggaacgta
    54961 tgtacgtata ccgaaggaac ataaaggcct tcttcaatta gaagatgagt tagggagtag
    55021 ttatttagca ttgttgccta ttggggaaac acctgaaaat atgagaaatt gtgatctatt
    55081 tcaatttgct caggatcttt ctttcccagc agaattgcga atgaaaattc actatccacg
    55141 cttgaaagga cagggaggta ttggtttaaa actgaattgg ctatcggaac ggttcaaaac
    55201 agagcataag gaattggttc aaaatgatga agaacctagt gaacgcttat taactgttcg
    55261 aggattagta aaacatttaa agcaacgagt agataatggg caacctgtcg tcgattggat
    55321 cgcatgtttt attgtatacg gtcaaacggt tgaagaagtt cgtcaaaata gtgcaacggt
    55381 tattcaacaa cttcatacga ttatccatat ccaccgtgca aaaaatgatc aacgagaatt
    55441 gttctataaa tgtttattag ggcaaccatt gggacggcag ttacattgga agcaccgaac
    55501 aactgtagaa gcagttggcg aatggttgtt ttcagcagta tcagaattag gtatggaatc
    55561 cggctggtat attggtcggc aagatacatt aggggatggt ggttctcgag gagaaaaaac
    55621 accattagaa gaattaattt atgcgtcaaa tcgttttgtc tttttgaatc ttctagccat
    55681 tgcggaagga atcaaaggtg cgttgtttga tgcgcctcat gtagcagtaa ctggaaaaac
    55741 cggtaaaggg aagacctttt tagttggctt gctctttttg tatagttcgt ttatgaatgt
    55801 acaaagttta tttgtggatc ccaaaggaga aaagcgcaaa tggtttacga aactcattca
    55861 agacccgtat tatcagaaac attacccttt gtttgtagaa catttgcaaa aaatgagtta
    55921 tgtcacgtta gatgctacaa atccccgtaa ttatggtgta ttagatccaa ttgtttgttt
    55981 atcccgagta gatgcgaaac aagtagccca ggatatgatt gatgagttaa gtccgttagg
    56041 taccaatcac cttttaaaag gggcggtatt aaagggaatc caagaagttt tagttcaaaa
    56101 agaagccgga caacaagttg gcttgatgca tgtggtcgac tatttagaac aaatggaaca
    56161 aaaagaggtt cgagaatatg gtgagttttt acgcttgacg gtagaagatt cgattttacg
    56221 tttaggattt tcttatggag aaaacccggg attagacttt aatgccaaaa caacgatttt
    56281 agagatccaa gatttgaaat taccaaatga caatgtccga ccagaactat atacagatgc
    56341 ggatcgaaag tctttgtgtt tgatgattag tttaggtcgt ttttgtgaaa tgtttggaaa
    56401 acgagatagt agtaagaaaa cagcgattta ttttacagaa gcatgggtat ttaataattc
    56461 gaacgcagga cgtagtatta ttgcagcaat ggcacgtgtg gggcgttccc agatgaatca
    56521 attagtgtta gatacacaat ttattggtga cttaggttca gataaagaaa aagggaattt
    56581 tggtgtcgtc tttgcttttg atgaagacag tgagcgagaa aagattttga atcatttacg
    56641 tttggaagta aatgaagcga acttagctga aatgagccac atgattaaag gacagtgttg
    56701 gatgttagat ccctatggac gtgtaggaaa actcaatgta cacagtttgt ttgaagaaat
    56761 gaccgctgca ttacaaacaa cggaaaaaac aagtcactct caagtagaag aacaggatac
    56821 gttaatcatg caatagaaat gaggtgaaac aatggcttat ataaagcata aggaaaagaa
    56881 atggtgcgtt ttgttttttt taacgattgg attcggactg ctttttgttg gtggattggt
    56941 gtatgcggca gaaaagcagg tggatcaatc gatctatgag attgggaaat ttcatattga
    57001 taccttttct tcttatttat ctgatagtga attttcatta ggcaaaatgt tatttggggc
    57061 aaatggcaca gattttctaa aaatgatgac gaatatgttc tttagtttaa gtaaattgat
    57121 ttggcaaggc cttgattttg tcattgagaa gctctatgaa ggagctgcaa tgaacgagtt
    57181 aattcaccag tttttcactt tttcggatac tatttattca aaaatatacg atcctattgg
    57241 tgcggggatt gtcgtcttgt atgtgttata tctatttact cttcgtatat ttagagggaa
    57301 acattacgca aaaatggtgt ttattcgatt tctcttcgtt gtcatttttt caatggtttg
    57361 gtttggctat ggtagtaacg tacctagtaa aggagaagct tttaccaaag ggttaaatga
    57421 tttgtccgtg gaggtagaag gctttatttt ttctgctaca aatagtctag atgaattggc
    57481 gacaaaagat gtcaatggtg ttttagtgcc aacgacacaa aaggaagcca ttcaaaaagt
    57541 gcgccaactt tattataaag cggctgttgt tgatccgtat atattgttaa attatgggac
    57601 aacaaattta gaagaattgt atcaagcaga gattgaacca caagaatttc tagccaagga
    57661 agcgtcaagt gaagaattat ctgatattga gacaaaagtg accactgctg caggagaaga
    57721 tagagggaaa aagaaggctt atcgtgtcta tattcaacca actaaagctg tttataaagc
    57781 agtcgttggt tctatggtgc cattgcttaa tctaagtatt ggattaccac ttgtagtgat
    57841 tggcatgatt cgttttatct ttcaaatgtt ggtgttgtgt ttattacttg tgattccttt
    57901 ttcattactc gcttcttttg ttccagtatt tgattatatg ctcatgaata ccttaaagtc
    57961 ctttttgggc ttgatgtttc aaaaatcttt ttacagtgtg attattttgg tggcattttt
    58021 gatctataac atagtagacg atttaattcc aatgaatact gtttttggtt ttttaggaaa
    58081 tatgtttgtc aaagcactcc tatcaatttt actcattgta aaacggaaag atttattaaa
    58141 gaaactaggg ttaggagctg cagatcaaac tataaacaac acgaaaaaca tagcaagtca
    58201 ttctttgtct caaatgaaac aactgcctgg tcaactatca actgctggac aaaatttggc
    58261 aatcaaaggt gcaggattag cagggaatgt gtatcgagca acgaatccac tcgcttctgc
    58321 agcgatacat ggatttcaaa agtttcaaca aaaacgtaca ccacaagaat cggtaaaagg
    58381 gaggcaagtg gttaatccct ttgaactagg tgggcgttca aatcaagcct tgccttatag
    58441 tggtcgtcat gttcgttatc atggggtaga gcctgaaatg tcttctacca aatcgaaaac
    58501 agagtggaat tctggtcttt caaatggttt gattccaaat tcgtccatga attcagtaag
    58561 accaaaacaa caggtacaat cattggcaca attgagtaag caacaaggga ataatcaaag
    58621 aaaaccattg ttaaataaag gtatcgcttt tgttggtcaa tcgattccgt tgccttctgg
    58681 aaaagtaccc gtgccacaat taacagctgc tggttctctt tcacaacaga ttcgccaagt
    58741 gaaaaatcag gcgaaaaatg ggagttatcc agcaatgcag cagttattga accaatactc
    58801 tgttcctaat tcgtcggtag ccactctatc aggaaatcgt tctgtgcaac aaccggggca
    58861 acgttcgtct gaaagtaaac cgtcgatttc cgcaccaaaa tcgttacaac ctgcattttc
    58921 ttctttagat aagccaacag agcaatcgga aaaaatagaa aaggatccta aaacactgcc
    58981 gattttggac gcaaaaccag gaactgattt tactttacca aaaggacgaa cagtccaaaa
    59041 accattatcg aagaaaggat gaggaagaaa tgaagaaagg gtgtctaggt tgtcttcttt
    59101 tctttattct tccctttttt ctattgccat tctttttttc aggagaactt tctgagcatg
    59161 gaggagaagc tacaggtcta gctgtcgctt ttaatgggga atataccgaa ggattaccga
    59221 ttttttcaga gattaaagga cgtggtccaa tcacagatga acatgcgcaa gcagcagttg
    59281 gtgcagctgt aaagtatcag ttattaccga gtgtgattct ttctcaatta ggctatgaat
    59341 cagcttatgg acagtcacaa tctggaagaa acgatacgaa cttttttggg attacctggt
    59401 ttcctggttg tccctaccca caaggttctg cacgtggaat tggtggttct gaaggtggtt
    59461 tctatatgaa gtttccgaac ttaaaagctt gttatgacta ttatggcttt atggtggcga
    59521 gccaaagcaa ctttaatcaa tgtgtgggca ataaagatcc tggaagttgt ttattgattc
    59581 taggtcgtgg aggctatgcg gcagcaggta ttaccgaagg gagtccgtat tatcgaggag
    59641 caatgagtat cataacgtcc tataacttaa cggaatatga tacttttgcg atcaagcatt
    59701 ggaatagcct accaacaaaa aaaacttcgc ttattgcagg aaaaagtaaa ggcaatgcga
    59761 aggttttgga tcggatcatt ggacgatccg taaataatgg ggaatgttat ggtggtacgg
    59821 catggtatgt atctcaatta ggtggcccac agttaatggg ttctggacac atgtttgcga
    59881 tgttaatagg ggaagattat aactggacaa cctatggttg gcaagtaatc atgaacccaa
    59941 aaccaagtga tttacaagca ggagatgtgg tgaattggca agcaggtggt gcattgtctc
    60001 ctggaatcta tggtcacacc ggaattataa cagatgtttc atatggcgga caacatttct
    60061 ctagtgtgga acagaatgcc gagcaaggac ggattatggc tcggtatcaa cgcacgtaca
    60121 atcaaacaaa aattcgtagt ctggttcgta aggtgaaata agaaaggcag tgactagaaa
    60181 cgtgaaaaaa gggataattt tagtatttag ttggttatgt ttgataggtc ttactggttg
    60241 tcattggttt tcttctgaaa aacaaaaaac acaattgaga acgacagaag aaacaatcga
    60301 agcagtaaaa gaaacaagtg gaactcttca aagtactgaa agtaacagtc aaattggttc
    60361 attagaagac gagacagcgc aacaaatgca aatagatgaa atgacaaaaa atttcttaaa
    60421 agattttgct agaaaatgga gtaatttcac agatgtttat aaacgcaatc aagctgtgag
    60481 ggactatatg acagatcgtt gtattaaaga caatagtatt gatgtagacc cgcatgtaca
    60541 aatgaaaaca actgggaaac tttaccaggt gacacaagat atagagaatc cggatcaata
    60601 tttgttattt ggagaagaaa caatgaataa ggttactcaa tttgtattac ttcaattgaa
    60661 agtaggtcaa gaagctcaaa agattgatgc gatcaagatt tattatgtac ggagtgcgta
    60721 ttaaaataaa aaatgaacac tcatatttat tatacaccga agaaatagaa aaaaacagac
    60781 tgttttttta agaaaaacta tgtcattctt tacttgaaaa aagttgttat aggacagaaa
    60841 ggataaaaca aaaacggaaa aaatatgtct tatgaataac tataagaact attctatttt
    60901 ttaggagatg gatacgagat gaaaaaacta attggctata gtgtgatgtg tattggattt
    60961 gttggtgtgg gattgtttgt atcgactgaa tctgcttctg cggctgaatg gtataatcct
    61021 atctggtata atccgaacat tgaatgtcaa aaaggctatc cgtatggacc gacaaatctg
    61081 aaaaatggaa aatgtagagt aaattggaat ggcgttgtag atagtgtaat tactggttca
    61141 atggactctg ctctacatgg ctttacgggt cactagaagg aggaagaaaa tgtctgaaga
    61201 gaaaaaagaa atcgttgaat cattagtagc tttaaaagat tctctttcta agattgaata
    61261 tgttgatagg caagaaattc aaaaactaat tgatgatact attattgaaa tccaagatgc
    61321 gcgttgtgaa ggtataaaaa tttctgtagc tttatctaaa gtaatagaaa aaatgaaccg
    61381 ttcattagcg tttaatggat tgaagctaga tagacaaact agtctgatat gggatcattt
    61441 aaaagacttg tatgacaagt ctaaaagaag tgaacggact gctgtatcaa tcttaaaagg
    61501 cctttgggga atgaattcat aaacaagata tttaaaaagt aacttatttt ttatggagga
    61561 gaaattatga aaagaataat taaaatattt ggtgttttag gtgttttctt attaggttta
    61621 agtacttttg ttagtactgg aacaagtgtg tatgccacag aaaaagtgaa tacagttgat
    61681 ttagaaataa agaataaatt agtagatgaa ttaacttttt attttgatac agttggtttt
    61741 ttagacgaac atggtgtata ccatataaag gattttggtg ctattcgtga acgaatcagt
    61801 caagataacg aacaaggaag agcagcaaga gtcttttatg aacatattat tgttcctaga
    61861 atggagaaag ttgattggaa acgatatgct ggatgtgtag ccattaattc tcttcctgga
    61921 ggaggagtgg cttgggctat aggtacgcag ggagatttta ttgaagcatt aaaagctttt
    61981 aattttgaaa aagctgcgga tttattatta aaaattgcag aaaaacaatt aggaaaagct
    62041 gcagctaaag agttagctaa ggtaaatatt gtagcaggaa ttgcagcagc tgctgctagc
    62101 tgtgcattag agtaaaagtt tatgaaaagt ttagaacaag agcatatggc ttttaaacaa
    62161 gcaatgttta aagaaaatat ctatttaaac cataactata ttcgtgtttc taaggcatgt
    62221 tcaccagtat tgaatatgct tggaggtgga aatggattat atcatttact gtttgtcgat
    62281 gtttgttggt taattttttt accagatgaa ctagttatag tcaatgaaaa aataacaagt
    62341 aaaaatgaag tgtttaatta ttctttaaca agaattaatt acaaagagat taccaagttt
    62401 tcactagaaa aagttccttt ttggggagaa ttttgtttaa aaattaaatg taactggaaa
    62461 agaatgtatt tctatattga tggtgatgat gctcttacat tcggtaaaac tactttttct
    62521 agttttaatt ttcagtttct attaaaaaat aatttttatg gtttattgaa ataaggttgc
    62581 gaaggaagat gtagagttat ttgcactgtt gtgttggtat atctcttaat gctgtgtctt
    62641 tttggggaga ttaattagtg gagtgattat atgagtatag atgattgttt agaagaacaa
    62701 ataagattat ttttaaagtt tttaaaagtt aaagttcaaa ataactactt cacatgttat
    62761 agttatgcac accctgtttt aaatagtctt ccttttaatt ctttagtgca aggattgatt
    62821 acaaatattg ttttattagt ctgtacacca aatgaaatat ttgtaaaaaa gataggcagt
    62881 ggtatttctt cagtaagatt aaaagagaaa gaatatactc aaaagattat tcatattcct
    62941 aagcctgaaa ttaagcgttt tgagataaaa gactattctg ctttagggct gttctcaatt
    63001 ggttactggt gataaaaaat aagaatacta aaactatacg tagaatataa gtaatcggca
    63061 tcaatcctaa ttatgtgatt gaatgccgat ttttgttacg cgctattttt aattatctgt
    63121 aagaagaata attttatttt actcatattc gttttcactt tgaagaagta aattgcaatt
    63181 tcttataagt taagcagcta tgcaaaaggc tatttattct attgtgaaat ataagatgta
    63241 gttgaaacag gatatataga atcaaaatac gaatcttatt ttgatttttc tagttttttg
    63301 aaaaaagcac cctttgatcg ttgtattagt ggaaaagaga aaaaggagca tacttatcat
    63361 gtcacaaaac tacaactaaa tatctaaaca acaccaattt gatgcctatt gaaaaaaagt
    63421 attgcgaaat gaagccaagt ctattagaaa atgaaataat agaataaaag aaaatgggaa
    63481 accgctgaat gatgtaaatg aaggtaagta tccacgaact aattttgatg aacaagatat
    63541 ctatttttta tttgtattgg agatactagt ttaatatcaa aagttatcag cagtcataat
    63601 tagttatcca atgtaaaaag tacaaacaat agttgttatg cagaatagtt tatgaaatat
    63661 attacataat aagaaaacaa gcgatatttt tctgatcaga acaaccattt aatttaaaaa
    63721 gagttaaaag ttatatattt ttgaaatatt ttggatgagt agctaaaaca atattttgct
    63781 cattaagcat taaatgaaga tgcaacgatg ttagaaagtt agcttcttct ggatcttgtt
    63841 ttgttaatgc ctcaaatagt tgataatgtt gttccataat tttttcccac tttaatcctt
    63901 ccgtttttac tcgtagccat cgaaatcgtt ctaagtgtgt attgttattt tctaaccatt
    63961 gccatacttc ttctcgctct gcaatttcaa aacaagcttt atggaataaa ttatcattat
    64021 gaaaaaaact acgagcattt ttttgactaa cagctttttc ttgttctgaa attatagttt
    64081 ctaaaatccg ttttttttgg ttattcattt tggcacaaca ctctaacata atttgtcgtt
    64141 ccaaatgttc tctgacaaat cgagcatttt tggccgaatc taaatcaatt aatgagatat
    64201 aatttccgct ttgaggaatt gtatcaacaa gaccttgttg tcggagttga atcagtgctt
    64261 cacgtattgg tgtacgacca atagaaagtg tttcttctaa ttttttttct gatacttttt
    64321 ttccaggttc taagtcagca tagataattt gtcggcgaat agattggtaa gcttgatttt
    64381 ggagactagt tttaattgtc atacacaatg ctccttaact tttattttaa ctgatatttt
    64441 aattctgttc atgccatata ata
//
LOCUS       DADVXU010000005        61702 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.005, whole genome shotgun sequence.
ACCESSION   DADVXU010000005 DADVXU010000000
VERSION     DADVXU010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 61702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.005"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(122..499)
                     /locus_tag="KB953_000309"
     CDS             complement(122..499)
                     /locus_tag="KB953_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865313.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            755..1483
                     /locus_tag="KB953_000310"
     CDS             755..1483
                     /locus_tag="KB953_000310"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBB6865314.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1483..1737
                     /gene="purS"
                     /locus_tag="KB953_000311"
     CDS             1483..1737
                     /gene="purS"
                     /locus_tag="KB953_000311"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HBB6865315.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1739..2410
                     /gene="purQ"
                     /locus_tag="KB953_000312"
     CDS             1739..2410
                     /gene="purQ"
                     /locus_tag="KB953_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HBB6865316.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2411..4633
                     /gene="purL"
                     /locus_tag="KB953_000313"
     CDS             2411..4633
                     /gene="purL"
                     /locus_tag="KB953_000313"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HBB6865317.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4618..6057
                     /locus_tag="KB953_000314"
     CDS             4618..6057
                     /locus_tag="KB953_000314"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBB6865318.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6080..7132
                     /locus_tag="KB953_000315"
     CDS             6080..7132
                     /locus_tag="KB953_000315"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBB6865319.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            7129..7707
                     /gene="purN"
                     /locus_tag="KB953_000316"
     CDS             7129..7707
                     /gene="purN"
                     /locus_tag="KB953_000316"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBB6865320.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7704..9239
                     /gene="purH"
                     /locus_tag="KB953_000317"
     CDS             7704..9239
                     /gene="purH"
                     /locus_tag="KB953_000317"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBB6865321.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9255..10529
                     /gene="purD"
                     /locus_tag="KB953_000318"
     CDS             9255..10529
                     /gene="purD"
                     /locus_tag="KB953_000318"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBB6865322.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10651..11565
                     /locus_tag="KB953_000319"
     CDS             10651..11565
                     /locus_tag="KB953_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBB6865323.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11698..13263)
                     /locus_tag="KB953_000320"
     CDS             complement(11698..13263)
                     /locus_tag="KB953_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBB6865324.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13256..14707)
                     /locus_tag="KB953_000321"
     CDS             complement(13256..14707)
                     /locus_tag="KB953_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBB6865325.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14721..15737)
                     /locus_tag="KB953_000322"
     CDS             complement(14721..15737)
                     /locus_tag="KB953_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBB6865326.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15957..17045
                     /locus_tag="KB953_000323"
     CDS             15957..17045
                     /locus_tag="KB953_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HBB6865327.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17099..18625
                     /locus_tag="KB953_000324"
     CDS             17099..18625
                     /locus_tag="KB953_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBB6865328.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18627..19832
                     /locus_tag="KB953_000325"
     CDS             18627..19832
                     /locus_tag="KB953_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBB6865329.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20089..21066
                     /gene="guaC"
                     /locus_tag="KB953_000326"
     CDS             20089..21066
                     /gene="guaC"
                     /locus_tag="KB953_000326"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HBB6865330.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21330..22754
                     /locus_tag="KB953_000327"
     CDS             21330..22754
                     /locus_tag="KB953_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBB6865331.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            23215..24324
                     /gene="pdhA"
                     /locus_tag="KB953_000328"
     CDS             23215..24324
                     /gene="pdhA"
                     /locus_tag="KB953_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HBB6865332.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24327..25304
                     /locus_tag="KB953_000329"
     CDS             24327..25304
                     /locus_tag="KB953_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HBB6865333.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            25329..26972
                     /locus_tag="KB953_000330"
     CDS             25329..26972
                     /locus_tag="KB953_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HBB6865334.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            26980..28386
                     /gene="lpdA"
                     /locus_tag="KB953_000331"
     CDS             26980..28386
                     /gene="lpdA"
                     /locus_tag="KB953_000331"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBB6865335.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            28548..28823
                     /locus_tag="KB953_000332"
     CDS             28548..28823
                     /locus_tag="KB953_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HBB6865336.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            28829..29596
                     /locus_tag="KB953_000333"
     CDS             28829..29596
                     /locus_tag="KB953_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HBB6865337.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            29764..31593
                     /gene="typA"
                     /locus_tag="KB953_000334"
     CDS             29764..31593
                     /gene="typA"
                     /locus_tag="KB953_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HBB6865338.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            31854..33626
                     /gene="recQ"
                     /locus_tag="KB953_000335"
     CDS             31854..33626
                     /gene="recQ"
                     /locus_tag="KB953_000335"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HBB6865339.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            33740..34042
                     /locus_tag="KB953_000336"
     CDS             33740..34042
                     /locus_tag="KB953_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HBB6865340.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            34045..35208
                     /locus_tag="KB953_000337"
     CDS             34045..35208
                     /locus_tag="KB953_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBB6865341.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            35234..38662
                     /locus_tag="KB953_000338"
     CDS             35234..38662
                     /locus_tag="KB953_000338"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HBB6865342.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            38683..39807
                     /locus_tag="KB953_000339"
     CDS             38683..39807
                     /locus_tag="KB953_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865343.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            39797..40234
                     /locus_tag="KB953_000340"
     CDS             39797..40234
                     /locus_tag="KB953_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBB6865344.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            40218..40556
                     /locus_tag="KB953_000341"
     CDS             40218..40556
                     /locus_tag="KB953_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HBB6865345.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            40675..41235
                     /gene="rsmD"
                     /locus_tag="KB953_000342"
     CDS             40675..41235
                     /gene="rsmD"
                     /locus_tag="KB953_000342"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HBB6865346.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            41232..41723
                     /gene="coaD"
                     /locus_tag="KB953_000343"
     CDS             41232..41723
                     /gene="coaD"
                     /locus_tag="KB953_000343"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBB6865347.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            41713..42756
                     /locus_tag="KB953_000344"
     CDS             41713..42756
                     /locus_tag="KB953_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HBB6865348.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            42915..43643
                     /locus_tag="KB953_000345"
     CDS             42915..43643
                     /locus_tag="KB953_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBB6865349.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            43759..44004
                     /locus_tag="KB953_000346"
     CDS             43759..44004
                     /locus_tag="KB953_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBB6865350.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            44001..44219
                     /locus_tag="KB953_000347"
     CDS             44001..44219
                     /locus_tag="KB953_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBB6865351.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            44355..44894
                     /locus_tag="KB953_000348"
     CDS             44355..44894
                     /locus_tag="KB953_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HBB6865352.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            44887..47160
                     /locus_tag="KB953_000349"
     CDS             44887..47160
                     /locus_tag="KB953_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HBB6865353.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            47198..48244
                     /gene="holA"
                     /locus_tag="KB953_000350"
     CDS             47198..48244
                     /gene="holA"
                     /locus_tag="KB953_000350"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBB6865354.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(48333..48587)
                     /locus_tag="KB953_000351"
     CDS             complement(48333..48587)
                     /locus_tag="KB953_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBB6865355.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(48987..49256)
                     /locus_tag="KB953_000352"
     CDS             complement(48987..49256)
                     /locus_tag="KB953_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865356.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(49726..50181)
                     /locus_tag="KB953_000353"
     CDS             complement(49726..50181)
                     /locus_tag="KB953_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HBB6865357.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(50319..52247)
                     /locus_tag="KB953_000354"
     CDS             complement(50319..52247)
                     /locus_tag="KB953_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBB6865358.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(52444..53277)
                     /locus_tag="KB953_000355"
     CDS             complement(52444..53277)
                     /locus_tag="KB953_000355"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBB6865359.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(53469..54173)
                     /locus_tag="KB953_000356"
     CDS             complement(53469..54173)
                     /locus_tag="KB953_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBB6865360.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(54196..54822)
                     /locus_tag="KB953_000357"
     CDS             complement(54196..54822)
                     /locus_tag="KB953_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBB6865361.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            55148..57523
                     /locus_tag="KB953_000358"
     CDS             55148..57523
                     /locus_tag="KB953_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HBB6865362.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(57547..58038)
                     /locus_tag="KB953_000359"
     CDS             complement(57547..58038)
                     /locus_tag="KB953_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HBB6865363.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            58225..59145
                     /locus_tag="KB953_000360"
     CDS             58225..59145
                     /locus_tag="KB953_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBB6865364.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            59299..59643
                     /locus_tag="KB953_000361"
     CDS             59299..59643
                     /locus_tag="KB953_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HBB6865365.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(59833..60333)
                     /locus_tag="KB953_000362"
     CDS             complement(59833..60333)
                     /locus_tag="KB953_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB6865366.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            60861..60944
                     /locus_tag="KB953_000363"
     tRNA            60861..60944
                     /locus_tag="KB953_000363"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:60895..60897,aa:Leu,seq:cag)
     gene            complement(61268..>61702)
                     /locus_tag="KB953_000364"
     CDS             complement(61268..>61702)
                     /locus_tag="KB953_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HBB6865367.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        20233 a        10662 c        13216 g        17591 t
ORIGIN      
        1 gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc aataatagga
       61 acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa aaatagagga
      121 attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga tgttgcagca
      181 agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg atacgataat
      241 gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct ttgtgttctg
      301 tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct cggcggattt
      361 ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg atcgacagca
      421 aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa agctgacctt
      481 gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa atttttagtt
      541 aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta taagattttc
      601 gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca tatttttgtt
      661 gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa aacgatgtat
      721 ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac ttttgtatga
      781 aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga tagaatattt
      841 agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa aaggagaatt
      901 gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa taaaaaatca
      961 ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa ttatccctct
     1021 ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag gtgttcccga
     1081 aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc agttggatga
     1141 tccatttatc aatgaagagc atatctattt cttacaactt gcaaagccgg aagaaatcag
     1201 tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat ttgctaaggt
     1261 cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac aaattttgct
     1321 tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga atgaacatat
     1381 ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc aagaagtata
     1441 taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt cgtaaaagta
     1501 tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa aaaagcagtc
     1561 catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt tgagatacaa
     1621 cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga caagctgctt
     1681 gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga agtataacat
     1741 gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt tatgggcagt
     1801 tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct ctttagaagg
     1861 atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga gatgcggtgc
     1921 gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag aagggaaacc
     1981 agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt tgccaggtgt
     2041 actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga aagtcaaccc
     2101 caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc cagttgctca
     2161 tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa aaaataatca
     2221 gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg cagggattac
     2281 gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag tagaagactt
     2341 gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact tcggaaaggt
     2401 gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga aaaaaagaat
     2461 ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag atatcttagg
     2521 aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg aacattgttc
     2581 ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc atgtgttgca
     2641 aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag ttgtcttcaa
     2701 agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg cgacaggagt
     2761 tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt tgctagattc
     2821 attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag aagtagttgc
     2881 aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg aaattgcatt
     2941 tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt tgattgaaca
     3001 caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt atgtaggtgc
     3061 aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat ttaatcaaga
     3121 agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa agctgctgct
     3181 tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta tacaggatat
     3241 gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg gctcaggact
     3301 tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt atgaaatgat
     3361 gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg aagaaaaaat
     3421 ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag taacagacga
     3481 cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag tcgatgcatt
     3541 agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa tcgtagaatt
     3601 tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat tgcttgatct
     3661 gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg attcacaagt
     3721 acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg ttagagggac
     3781 gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt tggatccaaa
     3841 aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta gcggtggtaa
     3901 gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag aagttttttg
     3961 ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag gaactcctgt
     4021 tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct atccaacacc
     4081 aatgattggt atggtcggat tgattgagga tcataaacat atcactactc aggaattcaa
     4141 aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg atggcagtga
     4201 attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg atctatccat
     4261 tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga ttgaaagtgc
     4321 tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt tcaaacatca
     4381 gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag aaactcaatc
     4441 aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa tgatgggaga
     4501 tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt cagcaactga
     4561 cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg caatcgtatg
     4621 tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg gggtcatcca
     4681 gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg acaagaaggt
     4741 gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt ggggctgctt
     4801 tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc tgcaatcgga
     4861 catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc ttttctattc
     4921 aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa tgctcgttcc
     4981 cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga tacggagata
     5041 ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt aaaaagtgct
     5101 ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc aatgattgcc
     5161 gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa cggagcatac
     5221 gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat aagagatgtc
     5281 cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg ctttacgaag
     5341 gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc agattcaaac
     5401 atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt agcgaaagaa
     5461 tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc agccgcaagc
     5521 ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa tcaatacatt
     5581 gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg aatgaaatta
     5641 tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga ttcaattgtt
     5701 cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc aaaagaagtc
     5761 catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat tgatattcaa
     5821 acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga atgtatcggt
     5881 gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg tctggaaaaa
     5941 gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc gacgccgtta
     6001 tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt cttttaaaag
     6061 cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag ctggcgtaga
     6121 tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa aaacggaacg
     6181 tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat cggtctttca
     6241 attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat tagcagtggc
     6301 aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt gtgtcaatga
     6361 tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa cggggaaaaa
     6421 tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg ttcaaggaaa
     6481 tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag gtaatgatta
     6541 tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga caggagaaac
     6601 gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt caaatgggta
     6661 ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat caaaactgcc
     6721 agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct atgtttcttc
     6781 cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca ctggtggagg
     6841 gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa tcaaacttgg
     6901 ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa ttcctgagat
     6961 ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcc aagaatcgaa
     7021 agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg aaatcgggaa
     7081 agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat gagaatcgct
     7141 gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct atcgaaaaaa
     7201 ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata tgttttgaaa
     7261 cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt tgattcaaaa
     7321 aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga tttgatcgta
     7381 ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga taaaagaatc
     7441 atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat acgtgatgcc
     7501 tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca aggtgtagat
     7561 actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac gttcgattct
     7621 ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat tagtgaaatt
     7681 attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca gataaaactg
     7741 ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt tcaacaggag
     7801 gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa gaagtgacag
     7861 ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc catggtggtc
     7921 ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa aatatccaac
     7981 caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg aaaccagatg
     8041 tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg ctccgtagtg
     8101 cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat gaagaagtac
     8161 tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa ctagcggcaa
     8221 aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta acggattggg
     8281 tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa acgcttcgtt
     8341 acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg gaaaaatatt
     8401 cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat atccgagatg
     8461 cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc gccttgaaac
     8521 acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg gaagcagcgt
     8581 atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga gaagtagatc
     8641 tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc ccttcttata
     8701 cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg acagtagact
     8761 ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt ggacttcttg
     8821 tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg acaaaacgta
     8881 agccgactcc agcagaagca aaagcattag cgtttgcttg gagagctgtc aaacatgtga
     8941 aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga gccgggcaga
     9001 tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa atggaaggtg
     9061 cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat gcagccaaac
     9121 atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat tctattgata
     9181 tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt agacattaga
     9241 gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa catgcaataa
     9301 gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca ggaaatccgg
     9361 ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat tcagcgttaa
     9421 tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa gttcctttgt
     9481 taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt ccgacaaaag
     9541 cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat acttatcaaa
     9601 tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg tatatcaaag
     9661 aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa ggtgtagtcg
     9721 tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac cagcatcgat
     9781 ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa gaattctccc
     9841 ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa gatcataagc
     9901 gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat tcaccagtac
     9961 cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa ccagcggctt
    10021 tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt ttgattgcta
    10081 cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct gaaacgcaag
    10141 tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta ttagagaatc
    10201 gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg gttgcagctg
    10261 ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg gaagaagagc
    10321 tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga gcaggaggac
    10381 gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa aaaatttaca
    10441 ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt ggagaaaaag
    10501 ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa aaaatacaac
    10561 aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt ggtacacttt
    10621 tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg tacttctgat
    10681 cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga agatccagta
    10741 aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc agatatttat
    10801 ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc aatcaaacga
    10861 gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt tgatttttct
    10921 aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat ggattatgtg
    10981 gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt agctgaagcg
    11041 tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa tcaaaaacca
    11101 atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa tcagctgcaa
    11161 tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat gacagatgaa
    11221 gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga tatgacgatc
    11281 caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg gaacgaaaaa
    11341 ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac gacaccaact
    11401 ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat cgccggtacg
    11461 atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat gtctcgggaa
    11521 gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt gtcataagca
    11581 ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt cagcttcgag
    11641 atattctgtc tttttttatt gcaaatggaa agagtatcat tgtacggatt tcgtttatta
    11701 gttatttttg tttttaagga aaagctgtgt ccgatattct tttggagaaa tcccttgttt
    11761 tttcttaaat aaataactga aatagttagg gtcattataa ccaatttcaa aagcaatatc
    11821 tgaaatacga tagtctgatt gctgcaataa tttttttgct aattcgattc tttgttctgt
    11881 cagataatcg atgaatgttt ggttaaacga ttgagaaaaa atcgtactga aatgagaggg
    11941 acttaatgca acttgttgag cgacccaagt taaggacata tctggatcgg taaaattttc
    12001 tttgataaaa tccaatgcat ggttcaaaat tggctgatat ttgttccttg aaggctgtga
    12061 gggcagagca gttaagaaag aaatgaacat ataaactgtc gaacggtaaa gcgagtcgtc
    12121 ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga accatttctt gcgggatttt
    12181 cttttctttc aggcagacat gctggagtag cttagtcaag acaaataaac gatataatcg
    12241 tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag atacttcgtt
    12301 tttcgataat gaagacaaat caagtgagag gtcaaagtaa aagacagaat caactggaag
    12361 tttcgcttct tgaatcaaat cctcatagct gataatttgt tctttaaggg aactggcggt
    12421 ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc tttctgcgac
    12481 aggaccaatg gcgacagaaa aattattttt ttgtcccttt tctaattcat ggaccaacgt
    12541 ttgggctacc tgataactgg tctcgatcac attttcttca agagaatcaa ctattaaaaa
    12601 tttgatgaat cgagaggaaa cagcagagaa gatcacttgt tgattctccc taaataaaaa
    12661 cttcaaataa tcactgagat gagcgtaatc ttcaaagtct gtactgactt ggtttgctgc
    12721 taataatacg gtcatttttt tccctgctaa agagcgattg aaagattcag caagtttcaa
    12781 tgtttcactg atcgtaagtg agcctttgaa caaaccgttc aagaaatgat tttttttcaa
    12841 ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta aagcagttac
    12901 tgctttttct attgcttgtt tcaattcgct acttttaatc ggtttaagca gataatcatc
    12961 tgcctgtacc tggattgctc ctttcatata atcgaaatcg tcgaatccac taataaatat
    13021 gagacgagtc caaggaagta gtttccttgc ttcttttgaa aaagtcaacc cgtccatgaa
    13081 aggcatttgg atatcagtca gaatgatgtc gggctgtaag tcgataattg aagacagtgc
    13141 catctcacca tctccagctt cgccgacata agtgattgga taataagaac caaggacagt
    13201 tatttgttcc cgcaagttat tgcgaatgag gtattcgtct tcgatgataa aaactttata
    13261 catgtttgtt tcctccaatt gttttgggag atagagggat catcagtgtg acagttgttc
    13321 cttctcggta tttgctgtca atcgtgatag gtttttgatc atttccgtaa taaagaagga
    13381 gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa atctgaatcg atccctttgg
    13441 ctaactcttt acgtatttct gccagttttt ctgcagggat gccaatcccg ttgtctataa
    13501 ctttgaattg gatcatctca tcaattttgc atgtggtaat acgaataaat cctttgtgac
    13561 ggacgtactt cgttccatgg tatacagcat tttcaatcaa tggctgcaag atcattttta
    13621 aaatagaaac aggaaaaagt gtttctggta tatcgatctt atagtcaagc atatggccgt
    13681 atcttatttt taagatgact agataatcat tgatatggct aatttctttt tcaagaggaa
    13741 tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca tgggtcatag
    13801 tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc aaactgttat
    13861 aaataaaatg gggagtaatc tgagcttgca atacacgaac ttcactttgt gctaaatggt
    13921 attgtttcaa agcgttctcc cgtaaagact ggtttagatc ttccgccatt ttattcatgc
    13981 tagcggtaag tgtttgtaat tccagtgttt ccggcagggt taaacgaaag ccaaggttcc
    14041 ctgaagaaag ttgttctgac atctcgatca gctgattgat cggttcttgg acctttctct
    14101 ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt actaagaact ccaagttcca
    14161 ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt gctaagtcga
    14221 tctccacacg aacaaaatct tgtaagatat ctgacagcag ttgagtgaca gaatctacct
    14281 gattgatgat ctcttcattg gtatccataa aatcttttgc caagatatta ttgatcagct
    14341 cgttttgata atcttccagt gtatcgatcg tacgtagcga tacatctaac gtactaattt
    14401 ctttaagtgt atttgtatta tttttgatct ctcgaatatc attcctaagt tcttctagga
    14461 tattttcctc attgaactca tctacggtta cttggccaga aatcaagtcc cacatacttt
    14521 ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata tttgcgatcg
    14581 tctgttgata aatcaaaagc tgttgcgaat ataacagtat gctcagtagc aaagggataa
    14641 cagcaaatag aagcatggct ttgtttgctt gggcaaaaat ctgttttatg cttaaatttt
    14701 ttgtcatgat acttctccag tcaataattt ctggttggga tgttagctat attctgctgg
    14761 gaaaagacag tctcggaaat aaaaatatct ttaggtaatg tttttcctgt ttgaatattt
    14821 ccaaaataac gagtaacggt attggctaca taccagccga catcaggttt acactcgact
    14881 acacaattga cgattcctga gcgtagcttc tcgatcatat cttcttgtcc atcaatagtt
    14941 acaatgatca aatcttctcc aggtttgatt gatgtttctt ttatcgcagc taaagcaccg
    15001 tgagtcattt catcactatg gctgtataat acatcgattt cggataaatc atttgtttct
    15061 aaatatcgtt tcatctggtc ttttcctttc atccgaataa aatcaccgac aattcggtga
    15121 acaatagtca tcggaggttt atggtcgatg gttccatctt tttttagggt tggtttacga
    15181 ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt ctctgaacca
    15241 gcaagttcta ggattctgat ttcctgtttc tttgaatttg tgaagtgatt gctgacaaat
    15301 aaacccgccc gatttccttc tgctttgaaa ctggggccaa tgtgggtgac gaaaagctcg
    15361 ggatcattcg tttgaatatg tctgtcgaca agtatgacag ggatccctgc tcgctttgct
    15421 tcttttaata cagagtccca cccggattct tgcaaaggag taaaaatgat catatccact
    15481 ttataggcaa tgaacgtccg tatatcttgg atttgccgtt cctgattcat gtagccattt
    15541 cggtagagaa cttggtaatc ttctttttca agcgcagtga taattgattc agtatgcttt
    15601 ttccgccagc tactctccgt tcctgattgc gaaaaaccaa cgactagtgg gtcaggtgct
    15661 tcctttgtat taggtgtaca ccccatcatt tggtaagcga ttacaacaag ggctaaaaaa
    15721 aataaaacac gtttcacttt aaatcactcc atctggattt gttagtacta tcttatcaga
    15781 taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa acttaaaaaa
    15841 atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa aaaatcaatg
    15901 ttaacgcttt ctttattagc tatactcggt gtatcaattg aaatggggga taaaaaatga
    15961 aaaacaaatt tagaaagatt gttgggacta tcgctatttt gaccgcaagc attctattaa
    16021 gtgcgtgtgg caattcagga tctgcagatg atagtacggg gtatgtagga atcgctatgc
    16081 cgaccaaatc agcagaaaga tggatcgctg atgggaacaa catggtctcg gaattagaaa
    16141 aactgggtta taaaacagat cttcaatatg gagaagataa agtagaaaat caagtagcac
    16201 aaatagaaaa tatgattaca aaaggggtag atacacttgt gattgcttct atcgatggtt
    16261 cggctttaac tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg atcgcctatg
    16321 atcgtttact gatgaattca gaaaatgtcg attattacgc gacttttgat aattttggag
    16381 taggtgtatc gcaagcagct tatattgaag agcacttagg attgaaagaa ggaaaaggac
    16441 cattcacgat tgaattattt ggtggctcgc ctgacgacaa caacgcacta atcaattaca
    16501 acggagtgat gtctgtttta caaccatata tggacaacgg tcagttggtc gtgccatctg
    16561 gacagacgag tttcagtcaa atcgctacgc ttcgctggga tggttctacg gcgcaagcaa
    16621 ggatggataa tctgttaagc gcaaattata ctgaccaaac attggatgct gttttgtcgc
    16681 catatgatcc aataagttta ggaatcattt cgtcattaaa aggcgtgggg tatgggagtg
    16741 aaagtaaacc tttacctgtg attactggac aagatgcgac agttgctggc gttaaatcga
    16801 tcattgccgg ggaacaaaca caaacgatct ttaaagacac acgtatctta gcaaaaaata
    16861 cgattgaaat gatcaaagca atcagtgatg gagaagaggt tccggtcaat gatactgaaa
    16921 cttatgataa tggggtgaaa actgtcccta cgtatttagc aaatacagta tctgtcgata
    16981 aagataatta tcaagcagag ctgatcgaca ccgattatta taaagaatcc gatctgaaaa
    17041 attaagagga accaaacaag gaactaaaga gatctaagtc agagagagga taagaattat
    17101 ggcggattgt attttaaaaa tggcagatat caccaaaaaa ttttctggaa tccgagcgtt
    17161 agattgcgtg aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg gagaaaacgg
    17221 agcgggaaaa tcaacattga tgaatgtctt gagcggcttg tatccatatg gctcctatga
    17281 cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag atagtgaaga
    17341 aaaaggcatc gtcatcattc atcaagaatt agcactaagt ccttatcttt caataaaaga
    17401 gaatattttt ctcggaaatg aacaagcaga aagaggcgtg attgattggg aactgacaga
    17461 aaagaaaaca tatgaactgc tgaataaagt cggtttgtct atcgacccga atcttctagt
    17521 atcccagatt ggcgtagggc aacagcaact agttgaaata gctaaagcat tatcaaaatc
    17581 cgtccgtctg ttgattttgg atgagcctac cgctgcattg aatgaagaag aaagcgcgaa
    17641 tttgctgaga ttgatccgag agttgaaaga gcaaggtgta acctccatca ttatttcaca
    17701 taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc attttacggg atggacagac
    17761 gattgagaca ctagaaaatg aaatgatcag cgaagagcat attattcgcg gaatggtggg
    17821 cagagattta gttgatcgtt atcctgaaag aaaaccggat attggagaag tttattttaa
    17881 agtagaagac tggacggtct atcaccctct tgatcacgag cgaatcataa acgaccacat
    17941 caacttgcat ttgaaaaaag gagagattgt aggaatagct ggactgatgg gagcgggcag
    18001 aactgaattc gctatgagtc tatttggaca ttcttatgga agcaagatat ccggacgtat
    18061 attaaaggaa ggggcagaga tatcagttaa gaccgttcct tcagccattg caaacggact
    18121 cgcttatgtc tcggaggacc gaaaatcttt aggactgaat ctgctgatgg atattcggga
    18181 aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa ggagtattag ataaagaaaa
    18241 agaagtaact gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa actccatcca
    18301 tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat ccaagtggct
    18361 gatgacacaa cccgatattc tttttttgga tgagccaacc cgtgggatag atgttggggc
    18421 aaaatatgag atctacacga ttatcgaaga aatggcagca aatggcaaag ctgtctgtct
    18481 tatctcttct gaacttcctg aaatattagg gatgtgtgat cgaatctata cgatgaacga
    18541 aggacgtatt acaggagaag tctcgcgaga agatgcagat caggaagtct tgatgaaatt
    18601 aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg gataaaaaag
    18661 agaaaaaact aaatttgaaa agtagagcag ctgatctttt tggtcgctac agcatggtga
    18721 tcattttagc gggactactt attgcgtttc aattgatgac ggatggtatt ttctggcgtc
    18781 cattgaatat aactaacctt gttctgcaaa atagccatat attagtacta gctgcaggga
    18841 tgttattagt tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg atggcgtttg
    18901 ttggtgcaat tgcgggcatg atgatggtca atcgccaaat gaacccgtgg atgacagtta
    18961 tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca aggtttttgg gtagcttatg
    19021 cagggatacc ggcgtttatc gtgactttag caggattgct gatgtttcgt ggactgacac
    19081 aagttgttct aggaggccaa tctctagcac cttttccagc tgttttccaa aaaatgtcta
    19141 cgggttattt accagactta agcgaaggaa gcagccttca cttcttgaca ttgattttag
    19201 gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat cgtcggaaaa
    19261 atcatttatt tgaagtggaa tctccaacag ctttcttggt aaaggcggta tttacagcag
    19321 tcgttgtact aggaataact tatatctttg cctcttatca agggtatccg attattttga
    19381 tcattcttgg tttgatcgtt gcagcatatg ggtttttgac aaataaaaca gtagcaggtc
    19441 gacagattta tgcgacgggc ggaaatcgaa aagcggctga actatcaggg atcaaaacaa
    19501 agcgaatcac gttctgggta ttcgtcaaca tgggagtcat ggcagcttta gctggattag
    19561 ttttagctgc tcgattgaat gcggcaacac ctcaagcagg tacatcgatg gaactggatg
    19621 caatggcggc agtatatttt ggtggtgctt cgacttccgg cggcattgga acaatcatgg
    19681 gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg attttaggcg
    19741 tcggggtaga ctggcagcag gcaatcaaag gattgatctt gcttttagcc gttgtgctag
    19801 acttgtataa taagaaaaaa cgaacaacgt aggagaacgc cggttattca gaaaaaagac
    19861 tgtgacaaga catttgtcac agtctttttt tcgctaaaag cgaacattca ttgtttcgtt
    19921 tgtatagact aaaaataata tatgtttttt taccattcgt ataaattctc tttttgctct
    19981 tgattttttt gcttaattga gttaaattac atagggcgct aaaaaaagcc ttgattaaat
    20041 taataatgtt cgtgttttgc tggatgtgaa catcaggagg ataatgtaat gaaagtattt
    20101 gattacgaag atattcaatt aattcctaat aaatgtatcg taaatagccg ttctgaatgt
    20161 gatacaacgg tcacattagg taaacataca ttcaaaatgc cagtagtgcc tgccaacatg
    20221 cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta tttctatatc
    20281 atgcatcggt ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa aaagcgtgga
    20341 ttgattacct cgatcagtgt tggcgtcaag aaagaggaat attcctttat agagaagttg
    20401 gcagaagaat cgctaaatcc tgattatatc actatcgaca tcgcgcatgg acatgctaac
    20461 tcagttattg atatgattca gcatattaaa aaatatttgc ctgaaacttt tgtgattgcc
    20521 ggtaatgttg gtacaccgga agccgtccgt gaattggaaa atgcgggcgc agatgcaaca
    20581 aaagtcggga ttggtccagg aaaagtatgt attacaaaaa tcaagacagg ctttgggact
    20641 ggaggctggc aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa gccgatcatc
    20701 gctgacggtg ggatcagaac acatggcgat attgcaaaat ctgtccgttt tggtgcgaca
    20761 atggtcatga ttggttcatt gtttgccggt cacgaggaat ctcctggcga aacaaaagtc
    20821 gaaaatggaa tcgtgtataa agaatacttt ggttctgctt cagaattcca aaaaggcgaa
    20881 aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt gaaagataca
    20941 ctagtggaaa tgcaacaaga ccttcaatca tctatttctt atgctggagg aagagatctg
    21001 gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa tggagacaca
    21061 atctaaaaaa taaataaaat attaggatgt tgtgaaaaag ttgtcctgca ttaagtaata
    21121 agaacagcca aacaaaaata gttctttata tgttttgttt ggctggaatt attatcgcag
    21181 atacaagctt ttgaacatca ttattcagaa taagggactg tgataagatt tttgtcacag
    21241 tcccttatgt tttgcaaaaa aagtttttct tttttctcgt aaattcggta aaatcaaaag
    21301 aaaaaataga caatagaaaa gagaagctta tgtgggaaaa agtagagaaa aaacaaggac
    21361 cgatttatcg tcaaattatg gaacagatta tgagagaaat cgaaaacgga aggatcaatc
    21421 ctggaagtca acttgaatca gaacgaaaac tagggcaact ttttggtgtg aatcgttcca
    21481 ccgttgtcca tgcattggaa gagcttaggg agcttggggt actaacgagc aaacgtggca
    21541 gcggatggtt tgttaatcag actgaatggg ggaaattcgc tgttccaaga atcgattggc
    21601 ggcaaatgat ctctcctcgt tatgagcaga cggactggta tgagcaaaaa ataagagaag
    21661 caaagcagat ccaaaaaaca tcgtttcttg atttatacgc tagcgagatg ccagatgagc
    21721 ttttgccgaa ttttgagttt ccttcttatt ctcttgaaga aatcttagcg gaagaaaaag
    21781 aaatggatct tttaggctat actccgttac gtaacaaaat caaacggtat ttggaaagta
    21841 aatttgaatt ggcattttcc cccaaccagc ttcttgtgac tggtggcggt cagcaagcaa
    21901 tctttttgat tttacaaacg attctttcag ctggagaggg gatagctgta gagtcacctt
    21961 ctttttttta ccgattacct cttttcaaag cttcaggaac gcgtttattt gggataccga
    22021 tggatgaaga agggatcgac ttagaaaaac tgaaagaatc gatccataaa aataaattga
    22081 aagcagtgct tatcaacccg aattttcaaa accctacagg aaaagtgatg agtcaaaaac
    22141 gacgagaaaa gctagtagaa ttgtgtcgac agtatcggct tccaattatt gaagacgatg
    22201 tatttacgga tctttcattt ctaaaccaag aagaagtcct tcctatacga acgattgatc
    22261 cggataatgt gttatacgtc ggttcgttat ctagggtttt aggaaaaaca acgaaaatcg
    22321 gttgggtgat aggacctgag acttttatct cgcaactagc tcaagcgcaa gaaatgatgg
    22381 agttttcgat gaatatcctt acacaaatca cagtggctaa tgtatttaat gaagcaattg
    22441 atttgaaaat gcaagatgta agaaaaaagt tgaaagaaaa aagccgactg ataagcgctt
    22501 ggggagattc acaatcgttt ttcaagcttc attcaatttt aggtggttac tatgcctgga
    22561 ttacttggga tgggaaaaaa ctagaccccg aactagcaga aaaagtagta gatatgggtc
    22621 ttggaattgc accaagtcta ctttttgggc aagatataaa tggaatcaga atcaattaca
    22681 gtaggctgga tgaaaaccaa ataccgttgt tttattcaaa gatgaaaatt ctagaaaagt
    22741 ggttaacaga ataatttcac aaagtaaata gattaaagaa tataacagct tggatttaaa
    22801 tttcaagctg tttctttttt ttcggaaaaa gaatacgata taaagaaact aagaaaaaac
    22861 cattatttgt acgtccgttc acgatgtgta aataaaataa atgtttgcaa caaaactgta
    22921 taacaccaac gtttttaaat agaaacagat ggttgtaagt ttgattagaa aatagaaaaa
    22981 atgcagatga aatataaaac gctttcctta ttgtgatttt attcactaat gctgttaata
    23041 tgcggattgg cataatttcc tttcatcgtg aaaatttgtg cttttagctt gtatttctca
    23101 taaatccatg ataatttatt aatgaagacg gttaaatagt gatacagttt tattcactgt
    23161 ttcaatcgca gaatgaagag attttcttat agatgagaga ggaatgtgtg acaaatggca
    23221 aacaaaaaag caccgatcga cttccaagca ctgctagatg aagtcaatgc agatttccca
    23281 gtatatcaag cacttgatca agatggaaac gtagttaaca aagatttagt acctgattta
    23341 tccgatgatg aactcgttga attaatgact catatggtat ggtctcgtgt attagaccaa
    23401 cgttcaacag cgctgaaccg ccaaggacgc ctaggctttt ttgcaccaac tgctggacaa
    23461 gaggcaagtc agctggctag tgcttacgca tttgataaag aagatgttct tcttccaggt
    23521 tatcgggatg tacctcaact agtaaaacat ggtttacctt tatctcaagc ttttttatgg
    23581 tctcgaggac atgctgcagg taattcttat ccggaagaat tgaaagctct accaccacaa
    23641 attatcatcg gtgcacaata tgtacaagca gccggtgttg cattaggttt gaagaaacga
    23701 aacaagaaaa atgtcgtctt cacttatact ggtgatggcg gttcatcaca aggtgatttt
    23761 tacgaagcaa tcaatttcgc tggcgcttac catgcaaatg ccgtatttta tatccaaaac
    23821 aacgggtatg cgatctctac acctcgtgaa aaacaaactg cagcaaaaac acttgcacaa
    23881 aaagctgtcg cagctgggat tcctggtatt caagtcgatg gaatggatcc gcttgcagta
    23941 tatactgtat ctaaaatggc acgggaatgg gcgatcagtg gaaatggacc agtcctaatc
    24001 gaaacactga cttatcgtta tggaccacat acactatctg gtgacgatcc aacccgctac
    24061 cgtacaaaag acacagatga cgattggcag aaaaaagatc cattgatccg cttccgtaaa
    24121 tatttgacgg aaaaaggact ttggtctgaa gaaaaagaag aacaagtcat cgaagcaact
    24181 aaagaagaaa tcaaagcagc tattgctgaa gccgacaaag taccaaaaca aaaggtttca
    24241 gatttcttga aaaacatgtt cgaagtatct ccacaatcaa tcaaagaaca aattgcaatt
    24301 tatgaagcga aggagtcgaa ataatcatgg cacaaaaaac aatgatccaa gcaatcacag
    24361 atgccttagc acttgaacta gaaaatgacg agaatgtcgt tgtttttggt gaagatgttg
    24421 ggaaaaacgg aggagttttc cgggcaactg aaggattgca ggaaaagttc ggagaagacc
    24481 gagtattcga tactccttta gctgaatctg gaatcgcagg attgagtttc ggtcttgcgt
    24541 tagaaggatt tcgtccagtt cctgaaatcc aattctttgg ttttattttc gaagcaatgg
    24601 acgaagtagt agctcaaatg gctcgtacaa gatatcgaat gagcggcact agaaatctac
    24661 ccatcacaat tcgttcgcca tttggtggcg gtgttcatac accagaattg cattcggata
    24721 atttagaagg tttgatcgct cagtctcctg gtattcgagt agtgattcct tccaaccctt
    24781 atgatgcaaa aggactattg atttcttcta ttagaagtaa cgatccggtt gttttcctag
    24841 aacacatgaa actatatcgc tcattccgag aagaagtgcc ggatgaagct tacgaagttc
    24901 ctttggataa agcagcagtt actcgtgagg gaacagatgt atctattatc acatacggag
    24961 caatggtgcg tgaagcaatc aaagcagcag acaatcttgc aaaagaaaat atatctgttg
    25021 aaatcattga tcttcgtaca gttgcgccat tagatgtaga aacgatcatt caatccgtgg
    25081 aaaaaactgg tcgcgtagtt gttgtccaag aagcacaacg acaagcgggc gttgccgcgc
    25141 aagtagtttc tgagatctca gaacgcgcta tcctttcatt agaagcccca atcggacgag
    25201 tttctgcacc tgatacagtc ttcccatttg gacaagcaga aaatgtctgg ttgccaaatg
    25261 caaaagatat cgaagacaaa gtaaaagaaa tcgcggaatt ttaagaaaag aagggacgaa
    25321 acaaagtcat ggcttatcaa tttaaattgc cggatatcgg tgaaggaatc gcagaaggtg
    25381 aaatcgtcaa atggttcgtc aaaccaggag atacgatcaa tgaagatgat acattactag
    25441 aagttcaaaa tgacaaatca gtagaagaaa tcccgtcacc tgttacagga acggttaaaa
    25501 atgtgattgt tccagaaggt accgtagcaa atgttgggga tgtattagta gaaatcgatg
    25561 caccaggaca cgaagacaat gaaggggaca gtggtgtagc cgcagaatcc caaactccag
    25621 ctaaaccggc tgctgaaccc actgttgata cagaatcagc tggatcgtca agcgaaggag
    25681 tcttccaatt taagttaccg gatatcggtg aaggaatcgc agaaggtgaa atcgtcaaat
    25741 ggttcgtcaa accaggagat acgatcaatg aagatgatac attactagaa gttcaaaatg
    25801 acaaatcagt agaagaaatt ccgtcacctg tcacagggac agttaaaaac gtgattgttc
    25861 cagaaggcac tgtagcaaat gttggggatg tattagtaga aatcgatgct cctggacaca
    25921 atagtgcacc gtcaactagt gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat
    25981 caggttcagc aagtgtcgtt gaagctgccg atccaaataa acgtgtattg gctatgccat
    26041 ctgttcgtca atttgcccgt gaaaaagatg tagatatctc acaagtaact gcaactggaa
    26101 aaggtggcag agtaacaaaa gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg
    26161 caccagcgaa atcagaagcg ccagaagcgg cagcaccgaa agaagcagct cctgcagcag
    26221 aaagcaaacc agctgcacca gcaaaaccat tcaagtccaa cttaggagac ttggaagaac
    26281 gtgtagcaat gacgccaaca cgtaaagcta tcgctaaagc aatggtcaac agtaagcata
    26341 cagctccaca cgtgacattg catgatgaag tagaagtgtc taaactatgg gataatcgga
    26401 aacgatttaa agaagttgct gctgcaaatg gtacgaaact tactttcttg ccatatgtcg
    26461 tgaaagcttt aacagcgact gttaagaaat acccagtatt gaatgcatct atcgacgatg
    26521 caaaccaaga aatcgtttat aaacattact acaatatcgg tattgcaact gatacggatc
    26581 acggactata tgtaccaaat gtgaaagatg cagaccgcaa aggaatgttt gctattgcag
    26641 acgaaatcaa tgagaaagca aaattagctc atgacggcaa actatctgca gaagatatgc
    26701 gcaatggaac aattacgatc agcaatatcg gatctgtagg tggcggttgg tttacaccag
    26761 tcatcaacta tccagaagta gctattttag gtgtaggaac gattgcacaa caaccaatcg
    26821 taaatgctga aggcgaaatc gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc
    26881 accgtatcgt agatggagca actgcgcaac aagcaatgaa taatatcaaa cgtcttttag
    26941 ctgatccaga gctattaatg atggaaggat gataaacaaa tggtagttgg agattttgct
    27001 gtagaattag atacagttgt aattggtgcg ggacctggtg gctatgtagc tgccatccgc
    27061 gctgctgaaa tgggacaaaa agtagcaatc attgaaagag agtatattgg cggtgtttgt
    27121 ttgaatgtcg gctgtattcc ttcaaaagca ctgatagctg caggacacca ttatcaagag
    27181 tcactagatt ctgaaatgtt tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa
    27241 acacaagaat ggaaagaaaa caaagttgtc catacgttga cttctggtgt tggtttccta
    27301 ttaaaaaaac ataaggttga aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca
    27361 ctacgcgtca ttcatccaga ttcagctcaa acttattcgt tcaataacgc aatcatcgca
    27421 acaggttccc gtccaatcga gattccagga tttaaattcg gaggacgtgt gttagattct
    27481 actggcggat tagcattgaa agaagtacct aagaaatttg tgatcatcgg tggcggtgtg
    27541 attggtgccg aattgggcgg agcgtacgca aacttaggag cagaagtaac gattcttgaa
    27601 ggctcaccac aaattttacc aacttacgaa aaagatctag taaaattagt agaagatgat
    27661 ttcaagaaaa aaggcgtaac agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac
    27721 ggtgacagcg tgacagtgaa atatgcagtg gacgggaaag aagaatccgt tactgctgat
    27781 tatgtgatgg tcactgtagg tcgtcgtccg aatacagatg atatgggctt agaacaagcg
    27841 ggtgtcgaag taggagaacg aggattgatc acggtggata aacaaggacg tacgaacgta
    27901 cctaacatct atgccatcgg tgatatcgtt ccaggggcgg cattagcaca caaagccagt
    27961 tatgaagcaa aaatcgctgc tgaagcaatc tctggcaaaa aagtagctgt agattataaa
    28021 gcaatgccgg cagtagcctt cactgatcct gaattggctt ctgtaggtat gacgatcaaa
    28081 gaagcaaaag atgctggtat cgaagcaaaa gcatataaat tcccattttc agggaacggt
    28141 cgggcattat cgcttggtaa aacagaagga ttcatccgtc tagttacgac gatcgaagat
    28201 aacgtgctta ttggcgcgca aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta
    28261 gctttagcaa tcgaatcagg aatgaatgct gaagatatcg ccttgacgat tcatcctcat
    28321 ccatctcttg gcgaaatcgt catggatgct tcggaattag cattaggttt accaatccat
    28381 atttgatttg aataatacag gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta
    28441 gaattatcaa taatattatt atgtaaactt aaaaatatga caacttcttg gtttattacc
    28501 acttcctatg ctacactgaa gaggaatatg aaataggaga gttgaagatg caagaatatc
    28561 agtatccact tgatttagat tggacaacag acgaaatggt cattgtaatg aagatgtggg
    28621 aagatctgga acgtgcgaat gaaaaaggga taaacaatga agcttttttg aagacttatc
    28681 agcagtttaa aactgttgtc aaatccattg gtgaggaaaa acgattggga cgggaatttg
    28741 aaaaagcttc cggttattct ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca
    28801 aaactttgag aatgaacggg tgagataaat ggaacagatg attgatcata tcaagtcctg
    28861 gcttgaagaa gctggtgaga aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa
    28921 atcaaatcga acggatctag taacaaatgt cgatcgaaac atccagaatt ttctaatcga
    28981 aaaaatccat tcctttgatc cagaggcgaa aatattggca gaagaagaag ggtgtaatca
    29041 attatcggat ctcgatggaa gagtctttat catcgatcct attgatggca ccttgaattt
    29101 tgtgattgaa ggggaaaatt tttgtattat gctcgctgtt tatgaatccg gcgttggaaa
    29161 gcttggtttc atttatgacg tgatgagaaa tgaacttttt tggggtggaa aaggaatagg
    29221 cgtgtataaa aatgatgagc cattgcctag accagcaaat aaatccttat cggaaggctt
    29281 acttggtgtg aatagttatc ttttcggtca taataaatat ggaatccgag aaattgcgga
    29341 aagcagcatg gggacacgta tgtacggatg cgctggtctt gaactagtgg caatcttgaa
    29401 aggaaaccac attgcttata tttcaaacct tagcccttgg gattatgcag cgggaaatgt
    29461 cttattggaa gagtttggta tggtaacaag cggtcttttt ggtactcctt tgacatttga
    29521 aaaaagggaa tactatttag cagggacttc ttgtgcatat gcagaaatcg cagacctttt
    29581 gaaaaaagaa ggctgaaaaa aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa
    29641 agattaattt tctcgaaaaa atgaaaacat tttactatat ttcatgaaaa aagtctgcta
    29701 taattagcag tcgagtaaaa tttgccctgt cataaagaac caataaaaaa ggagttagtt
    29761 acattgaatt atagaaacga tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga
    29821 aaaacaacat tagttgatga actattaaaa caatcagata cattagacgc acatacgcaa
    29881 ttgcaagaac gtgcgatgga ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta
    29941 gccaaaaaca cagctgtaga ctataaaggt attcgcgtaa acatcatgga tactccagga
    30001 cacgcggact tcggtggcga agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg
    30061 gtcgtagatg cgtatgaagg aacaatgcct caaacacgtt ttgtattgaa aaaagcactg
    30121 gaacaacata tcacaccaat cgttgtcgta aacaagattg acaaaccttc tgctcgtcct
    30181 gagcatgtcg tagatgaagt tcttgaacta ttcatcgaat taggagcaga tgatgatcag
    30241 ttagactttc ctgtgattta tgcatctgca ctaaatggta cgtcaagctt gtcagatgat
    30301 ccagcagatc aagaaccaac catggcacct atttttgata caattattga aaaaatccct
    30361 gctcctgtcg ataacagcga cgaaccatta caattccaag tttcattact tgactacaac
    30421 gattatgtag gtcgtatcgg aatcggacgt gtatttagag gaacaatcaa agtaggagac
    30481 caagtagcat taattaaatt agacggaaca gttaagaaat tccgtgtaac gaaattattt
    30541 ggtttctttg gactaaaacg tctagagatt caagaagcaa aagctggaga cttgattgct
    30601 gtctcaggta tggaagatat ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat
    30661 gcgttaccta tccttcacat tgacgaacca acattgcaaa tgactttctt agttaataat
    30721 tcaccatttg ccggtcgaga aggtaaattc gtaaccgctc gtaagattga agaacgtttg
    30781 atggcagaac ttcaaacaga tgtatcctta cgagttgaac caacaaactc tccagatgca
    30841 tggacagttt ctggtcgtgg agaattgcat ttgtctatct tgattgaaaa catgcgccgt
    30901 gagggatacg aattgcaagt atctcgtcca gaagttattg aaaaagaaat cgacggtgta
    30961 aaatgcgaac cgtttgaacg cgtgcaaatc gatacaccag aagaatatat gggtagtgtt
    31021 atcgagtcct tgagcttacg taaaggtgaa atgcaagata tggtccatac aggaaacgga
    31081 caaatccgtt tgacattctt aacacctgcc cgcggtttga ttggttattc aactgaattc
    31141 ctttcaatga cacgtggtta cggaatcatg aaccatactt ttgaccaata tctgccaatg
    31201 cttcctggtc aaattggcgg acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa
    31261 gcaacgactt attctatcat gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt
    31321 acagaagtat atgaaggaat gattatcggg gaaaacagcc gtgataatga tttgactgtc
    31381 aacatcacga aagcaaaaca aatgacaaac gtacggtctg ctactaaaga ccaaacatct
    31441 gtcatcaaaa aaccaaaaca attgacgttg gaagaatctt tggaattctt gaatgatgat
    31501 gaatattgcg aagtaacacc agaaagcatt cgcttgagaa aacaaatttt agagaaaaat
    31561 gctcgtgaaa aagcatctaa aaagaaaaaa taatctgtaa aaggatgaag agagaaacca
    31621 gcttgttata aaaaatagct ggtttctttt tttgtccaac tcttttctgt ggagaagtca
    31681 tctttgtcaa tggacataaa ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa
    31741 aaacttattt ctagagagtt tttgtttcat aataagataa tgtttcacga aaggagatca
    31801 acttgtcctt cgaagaaaat cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg
    31861 acttattaaa acaatacttt ggctatgatg agtttagacc tggtcaaaaa gaaattatcc
    31921 agaaagtcat tgatcaggaa aatgtattag gaatcatgcc taccggaagt gggaaatcaa
    31981 tctgttacca gcttccagct ttactattag atgggcttac tgtggttgtg tcccctttaa
    32041 tctctttgat gaaggatcag gtggacgcgg cgaatcaatt aggtataccc gccactttta
    32101 tcaatagctc gttagatggt tacgaaacag ctagacgctt ccaagaaatc gatagacagc
    32161 agtaccgttt actttatatc gctcctgaac gttttatcat gccggatttt atccaagcaa
    32221 tgaagcgatg gaacgtccgt atgatcgcta ttgacgaggc acattgtatc tctcagtggg
    32281 ggcatgattt ccgcccaagt tatttacaga tggcgaacca gctagatcaa ctgccaaatc
    32341 gtcctgtaat tgttgctttg acagctacag cgactgtgca agtagctgct gacataaaaa
    32401 gacttttaaa aattccagaa aacaaccata ttcagactgg ttttgaacga gaaaatctac
    32461 gatttcaagt aattaaagat caaaaaaaag aacaatatct aatcgaatat ttaaagatca
    32521 acaaaaatca atctggtatc atttatgcag caacgagaaa agaagtagac cgtctctatc
    32581 atttgcttaa aaaattcgat ttttccgtgg gtagatatca tggtggtcta aatgagaatg
    32641 agcgaacaga aatgcaagaa gcctttttat acgatcgctt gcagctgatt gtagcgacaa
    32701 atgcatttgg tatgggtata aataaaagca atgtccgttt tgttatccat tatcaaattc
    32761 ctggctctct agaagcatac tatcaggaag ctggtcgggc gggaagagat ggtctttcca
    32821 gtgaagccat cctgcttttc tctcctcaag atatccaagt ccaaaagttt tttatccagc
    32881 agtcccaaag agaagaaggg caaaagcaaa aagaatatga aaaattaaaa gcgatgacag
    32941 aatacgtgta tattgagtcc tgtcttcagc aatacatcct aaactatttt ggcgagacaa
    33001 gcagtccgtg caaccgttgt ggtaactgtt tagatgacag agaaatagta gaagtaacga
    33061 cagaagcaca gatggttctt tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa
    33121 tgctgatgaa agtcttagca ggatcgaaag aacaaaagct gcaagcttta ggttttggac
    33181 atttatctac ctatggactg atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg
    33241 aatatttgat atcgaatggc tatcttctga ccatcaatgg tgagtatcct gttttgaaag
    33301 taacagaaag agggatacaa gtattgaaag gacaagaatc tgtttatcga aaggaaccca
    33361 agaaagtcca gcagctatcg gatgaagaaa ccgatacact atttgaagtg ctgcgggaac
    33421 tgcgaacgga tctagcatca gaagcaggcg ttcccccgta tgtcgtattt tcagattcca
    33481 cattgaaaga aatgagccga atacgtccat caagcagatt ggagatgctt cagataaaag
    33541 gagttggcca aagcaagctt gataaatatg gcgaagcctt tttatctcgg ataaaaaatg
    33601 cggactctaa cgttctggat aagtagaaag aacgaatacg taaaaatgaa taaaaacaac
    33661 tggttaaatc tgtttttttt gctccttatg aaaatcaaat atgttataat taaggaatta
    33721 gaatgggagg taagcacaga tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa
    33781 tgatgaagca gaacggatca aacgattaat ccgaaatcaa aacaacagtc tctgtatctc
    33841 acaatgcaaa gccttcgaag aagtggtaga tacacagatg tacggctttt cccggcaagt
    33901 cggttttgct gtccgtatag gagtaatcgg aaagaacgag ggccagctga tgttaagtga
    33961 gctagaacga gaattgaatc agttatatag tgaggtttac caagaaattt atgataaaaa
    34021 agaagttggc aaggaggaat agacttgcct aagaaagtga aaaaaaggca tctattagac
    34081 tatagcatct tgattcccta tttaatttta tgtgtattgg gattgatcat ggtatatagt
    34141 tctacgtcgt atctattatt agaaaacgga caaaatccgt ccgcatcagt cattaaccag
    34201 agtatttttt gggtgcttag cttgattgct atcgcactac tttataaaat gaaaacagat
    34261 gttttgaaaa accagcgttt gatcatggca gccatcgcag ttttgacgat tttgctgctg
    34321 attgttgtgt tttttggtaa ggagatcaat ggggcgaaag gttggctgca aattgcagga
    34381 ttctcgatcc aacctgccga atacctaaaa atcatttcta tctggtacct gtctctgact
    34441 ttatctaaaa gacaaaacag tgtacaaaaa gattttcttg ggactgtgaa acgtccactt
    34501 gcaatggtta tcggacttac tgcattagtc gctattcttc ccgattttgg taatgcggca
    34561 gttatctttt tgattatttt ggttctgctt ttagccagcg gagtcaatta tgtgtatact
    34621 ttgattgtag gagtaggagg cttttgtcta agtactttta cgatctggct gatcaatatc
    34681 acaaacggaa agatcttacc cggacgttta cagtatatct ataatcgatt tgctatttac
    34741 caaaatcctt tctcagacga attaaataaa gggcatcaac tagtcaacgg ctactatgcc
    34801 atgttcaacg gaggactatt tggcagggga ttaggtaaca gtatccagaa aaaaggattt
    34861 ttacaagaag cacaaaccga ttttatttat gcaatcgtag tggaagaact gggagtgatt
    34921 atggggattt tgatattagc gttgctattc tttatgattg tgcgcattgt ccttgtaggt
    34981 atccgttcaa aagatccgtt caattcgctt ttgtgcattg ggatcggcgc aatgtttttg
    35041 atccaagtat ttgtcaatct tggaggaatt actggcgtta ttcctttgac agggatcacg
    35101 ttcccatttt taagtcaagg aggctccagt ttgctgatgc tttcgatttg tgtcggcttt
    35161 gtcctcaaca tcagtgcgga tgaaaaacgc aagagcttag gtttataata aaaaaagatg
    35221 taggagtggt tcgatgaaaa aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt
    35281 atttcgtgct tgtacagaat taggaattaa aactgttgga atctatgcag aagaagatga
    35341 atattccgta caccgtttca aagcggatga agcatattta gtaggacaag gaaaaaaacc
    35401 gattgatgcg tatctagata ttgaagggat catttctatc gcgaaagaat gcggagctga
    35461 cgctattcat ccgggttacg gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga
    35521 agaagaaggc atcatttttg ttggacccaa actgcatcat ttagatattt ttggagataa
    35581 aatcaaagca aaagctgcag ctatcgaagc ggggattgct tcgattccag gcacagatgg
    35641 accaatcgct tctatagacg atgccttgga gtttgcgaaa caatacggtt atccgattat
    35701 gatcaaggca gctctaggcg gcggcggacg cggcatgcgt gtagctcacg atgaaaagag
    35761 tgcaagagag ggctacgaac gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga
    35821 agtgtatgtt gaaaaataca tcgctaatcc taagcatatc gaagtacaga ttctcgggga
    35881 tacgcatgga aacgtcatcc atctttttga aagagattgt tctgttcagc gccgtcacca
    35941 aaaagtagta gaagtagcgc cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg
    36001 ccaggcagct gtccaattga tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt
    36061 tcttgttgaa ggagatgatt tttactttat cgaagtcaac cctcgcgtcc aagtggaaca
    36121 tacgatcaca gaaatgatta cagatattga tatcgtaaca acgcaactgt tgattgctca
    36181 aggtttggat ctgcataaag aaatcggatt gcctcagcaa gaaggaatta aattaaatgg
    36241 ttcagccatt caatgccgaa tcactacaga agacccgttg aataactttt taccagacac
    36301 agggaaaatc gatacgtatc gttcacctgg tggttttggc gtacgtctgg atgtcggaaa
    36361 tgcttacgcg ggctatgtag tgacaccgta ttttgattca ttgctagtaa aagtatgtac
    36421 gcatggtgct acctttgaaa cagccattca gaaaatggaa cgttgcttga aagaatttag
    36481 aatccgtgga gtgaaaacaa acattccatt tatgctcaat gtcatcacac atcctgaatt
    36541 ccaatcagga aatgcaaaaa caaccttcat tgatagtaca gcaacattat ttgaatttcc
    36601 tcgactacga gaccgcggaa acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg
    36661 ttttccaggt atcgaaagtg gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga
    36721 tctaatcaca agaactgatt acgtaacggc taagaatgta ttagatgcga agggtgccga
    36781 tgctttggtc gaatggatca aaggacagga aaatttgttg ttgacggata cgacatttag
    36841 agacgcccat caaagtctgc tagcaactcg tgttcggaca aaggatttca agcagattgc
    36901 acgtttgact ggagaaggtc ttcctgaact cttttcaagt gagatgtggg ggggagcaac
    36961 atttgatgtg gcttaccgct ttttgaatga agatccttgg caacggttga gaaaaattcg
    37021 ttctttaatg cctaatacgt tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta
    37081 ttctaattat cctgacaatg tattggtgga atttgtcaaa gaagcagcag cgcaaggaat
    37141 cgatgtattt cgaatctttg atagtttgaa ctggactcca caaatggaaa aaagtatcca
    37201 agctgtacgg gatactggta aaatcgcaga ggcagctatt tgctatactg gcgatatcaa
    37261 cgatccaagt cgggcaaaat acaatgttca gtattataaa gacatggcaa aggaactaga
    37321 acaactgggt gcgcatatca ttgcaatcaa agatatggct gggctgctta aaccgcaggc
    37381 agcttatcgt ttgattagtg aattgaaaga aacgacagat ctgccaatcc atctgcatac
    37441 tcatgatacc agcgggaatg gaatcatcac ttattctgct gcttcaaaag caggagtaga
    37501 catcgttgac gtggcaatga gtgcaatgag tgggaatact agtcagccta gcatgagcag
    37561 cttgtattat gcactggtca atggtccacg gctgccagaa atcacgattg aaaatgcaca
    37621 gaaattaaac cattattggg aagatgtgcg catgtattac aaaccgtttg aaaacggctt
    37681 gaatgctcct gaaacagaag tatatatgca tgaaatgcca ggaggacagt attcgaatct
    37741 acagcaacag gcaaaagctg tcggattagg tcatcgctgg gatgaaatca aacaaatgta
    37801 tcatactgtc aacttgatgt ttggcgatat cgttaaagta actccatcat ctaaagtagt
    37861 tggagatatg gcactattta tggtccaaaa tgatcttaca gaagaagata tttatgaaaa
    37921 aggcgaaaca ctaagtttcc ctgaatctgt tgtgactttc ttccaaggag aactagggca
    37981 acccgttggc ggatttccta aaaaattgca aaagattatt cttaaaggca gacctgcatt
    38041 gaatgaacgt ccaggtcttt tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt
    38101 ggcagaaaaa attggctatg aaccaaaaca agacgaagta ttaagctatt tgatgtatcc
    38161 gcaagtcttt ttggactatc aaaaagcata taatcaattt ggcgatgtta cattactgga
    38221 cacaccaaca tttttccaag gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg
    38281 cggaaaaacg cttatcattc gtttagatga aatcggagaa cccgatatcg aagggaatcg
    38341 cgtactgttc ttcaatttga atggacaaag acgtgaaatc actatcaaag ataattcgat
    38401 catcagtacg gtccaaaccc gattgaaagc tgaaccgaca aaccgagaac aaattggagc
    38461 aacgatgtct ggttctgttt tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg
    38521 tgatacgttg atgattactg aagcgatgaa aatggaaacg gccatcgaag cacgttttga
    38581 cggagaagtc gcacatgtat atgtgtcttc aggagacact atttcgtctg gtgacctttt
    38641 gattgaagtg actgagaaat aataagcgaa aggtggggaa acatgaaaag actgggtttg
    38701 ttttggctga ttctgctatt agttattgtg gcattctaca tggaaccagt cttgtttccg
    38761 ccaaaacttg aagtgaagga aaactctgat caacaaatat caaaccaaat gacaacgtgg
    38821 aaataccagg agcttaaagc aactggtttt tctacttata tcggaaaacc gatcgatcag
    38881 ctggaaaagg attttggcca agcctatgat cgattaacta gcggatttgg ttttgaaaca
    38941 agatattatg aaaataaagc tagtcatctc acatttgaag cgaacatcca agacagcaga
    39001 gtcacaactg ttaaagtact gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg
    39061 atgacgatgc aagatcttac gaaaatcact actatctata ctaatttcac ttttgagtac
    39121 catgatacag aagttggaat cgaactaatg gaagaggata tgaattatcg tccgctaatt
    39181 gcttttgata acgacacttt tgctatttta ttttttgatc aaacaacaaa cggcctattt
    39241 tcagttgtgt atttagataa agatagtctt ttgaaactat tgccatatca agtgtttggt
    39301 gaaggccttc cacattatca agcagataaa tcggctgatt gggaatcaat caatcgcgtt
    39361 aaagaacgaa aaagtacgac tttattaaat atgcttagaa gagaagacga acttcccata
    39421 tacaatcaaa cacttgcctt tcaaaaccac agtcagctgc tactacatga ttttttgaca
    39481 aggcctgagg aaatattgtc agaggaacgt atggctgatt ggcaaaaatc gctaggcagt
    39541 gctcaaacga cagtgaaatt ctcactgacg aatgatgaat tggagaagtt aatcagtaaa
    39601 caaaaattag aaaatactag tgggctattt attcatccgg cgattgacgc tactttttcg
    39661 tttttatatt ggtacagcga tccttacaac catgaccgtt tcatggaccc gggaacggat
    39721 tctgtaggca ttgctttttc taaagaaaat atgttagtct tattgcaaga agaaaacgga
    39781 gaaagtagtg atagcagtga tcgttaacga agagctgttt caactggagg atcaatgcct
    39841 ccaagtcgct gaaatgataa aaaagagtaa aacgatggct tcttatcttg aacataaaca
    39901 gtcaatggaa gaatctgcaa aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga
    39961 agcatttgaa aatgtagcgg catatggtga gtatgcacct gattatcgtg aaaaaaaacg
    40021 agctgttcga aaagcaaaaa gacagcttga tatggatgag catgttgcag ctttccgata
    40081 tagtgaaaca cagctgcaaa gtctgttgga tcaaattgtc caagtacttg ccgaagaagt
    40141 ttctaatgaa atcaaagtag atgcaggtag cccgtttttt gaaacgaaaa aacactttgg
    40201 atgcggaggt aattgtcatg caattagaca atagtgaatt tgaaataaca aaacgacgcg
    40261 ggctgatcgt atgggtctac agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt
    40321 tgcattatgt ctcaagaaga atgaaatatg tcgtgcttta cttaaatgaa gagacattgg
    40381 aagaaacaga agaacgaatc cgtaaattac attttgttcg aaacgtagaa cgttcttacc
    40441 gaccggatat cgaaatgaat tttgccgaaa aaataggaaa caaaacagta ttaaaagacg
    40501 aaggtttcga aattgaagag ctaaatacag aaatccgatt agctgaacat ctttaaaaat
    40561 ctcttatgag cagtttttaa ataaaaaata acttggataa cgatcgattt ttgatcattg
    40621 tccaagtatt tttttggtat actagtacca gtaccatttc taaggagtga gtagatgcgg
    40681 ataatatcag gaaattatgg cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga
    40741 ccaactacag ataaagtaaa agaatccata ttcaatatga ttggaccata ttttgatgga
    40801 gagacagtat tggatctgtt tgcaggaagc ggtggtttag ctatcgaagc agtatctaga
    40861 ggctgcagtc atgcggtatg cgtagacaaa aattaccaag cgctaaaaat catcaaagaa
    40921 aacatcgaaa ttacgaaaga accagagaaa tttaccacaa taaaacttga tgcggacaaa
    40981 gcaatcgaac ggctagcagc ggagaaacaa caattcgact acttgtttct tgatccgcca
    41041 tatgcaaaac aaaaaataat cgatcaaatc cataaaatgg aagaactggg acttttttct
    41101 aaaaccgcag ttatcgtatg cgaaacggat aaaacagtcg aattgccaga aactgtggct
    41161 ggattccatc aaataagaaa acagatttat ggcataacgg ccgtaactat ctatagaaaa
    41221 gagggaaatc aatgaaacgt gcattattcc caggaagttt tgatcctttt acaaaaggtc
    41281 acttagatac ggtggaacgt gcggctaaat tattcgatga ggtagtaatt ggtgttttta
    41341 tcaatacttc aaagaaaagt ttgtttcccc cagaagaaag aatgacattg atcacaaaag
    41401 ctgtttctca tcttcctaat gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa
    41461 cagcaaaaga gattggtgca gatgcgttga tcagaggcat acgaagtatc aaagactttg
    41521 aatatgaacg agagattgct cagatgaatc atcatctata tgatgaattg gaaactgtct
    41581 tcttattagc aaaaccagaa tatagtcacg tgagttccag tattttaaaa gaagtactgc
    41641 actttggcgg agatgtgagc agctacttac cgccagtgat caacgaagct ttagctggaa
    41701 agcgtgaatc aaatgaaagc taaacaatgg ttgaaaacag tactgattat catatttgga
    41761 atgttgatca ttggatttgt agtagttccc ttgccgtatt atgtagaatc accgggagcc
    41821 acgatcgatc tgaaagaact aattacggtc aatgataagg aagataaagg ttctgggtct
    41881 ttctctctta cctctgttgg tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa
    41941 atgacccctt ttgaagaatt gatcagtgaa gaagaattga ctggtggggc gacaaatgaa
    42001 gaatataatc aaatgcaaca gtattatatg gattcttccc aaaatgcagc aatcgaacaa
    42061 gcgttaaaac ttgcagacaa gccttataaa atggaattca aaggcgtata tgtcatgagt
    42121 gtagagaaaa catcgaattt ctttggcaag ctttcagttg gagatacagt aacaaaagta
    42181 gacgggaaaa gctttcaaag tacagaagca tttatggatt atgtaaaaag ccaaaaagtt
    42241 ggacaaacaa tcgaaatcag ttatttgcat aatggtgaag aaaaaacagc ttctggtgat
    42301 ctgatcgagt taccaacaga taaaaaggca ggaataggta tcacgcttac tgaccatacc
    42361 gagatcgaat cagatacaga tgttcgtatt aattctggct ctatcggtgg gccatcagcg
    42421 ggattgatgt tcactttaga aatctatgaa caagtcactg gtaaaaactt gcgacatggt
    42481 aagcaaattg caggaactgg aacgatcaac agtgaaggag aagttggccg aatcggagga
    42541 atcgataaaa aagtggcaag tgcggataaa gctggcgtag agattttctt tgcaccagat
    42601 gatacgattt cagacgatgt taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa
    42661 gcaaaagctg ccgctaaaaa attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta
    42721 caagaagcgc ttgactatct agagaaaatg aaatgaaact aaataattaa agaggggttg
    42781 tgacacgagt tctgtcacaa cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa
    42841 acaaaagaaa aaattgacgt aattagatgt tgaagggacg attttgccag agctcactgt
    42901 ttcttcaatt ttaaatgaaa ggagtaatga atgattttta ctggtcattc tttgaaaaaa
    42961 gaacaatgat agaaaaaatc agcaagaaaa tactagcttc acccaaattg atcatttgga
    43021 tgtctatgat agttcttatc ttttgtatcg caagtggttt tttattatca aatttgctat
    43081 tttatagaga aacagaagag actgctgcaa aagaaatact ttcttcgttt agttccagtg
    43141 gagaagatcc atcagctgaa ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt
    43201 acaccgatat caaaggaagt gtgaaggagc ctggtatcta ttccttttct tcagaagaac
    43261 gggtctatga tgtactaaag cgtgcaggtg gattattaga agaagcggat agcgatcgaa
    43321 tcaacttttc tgcgaagata gaagatcagc aagtcctata tatccctgct gtaggtgaag
    43381 agctgccaga acatcttgat cagttggcat cctcggaagg aaaacagcct acagcagatt
    43441 ccgagcctcc caaaataaat atcaatacag ccactccatc agaacttcag cagattcctg
    43501 ggatcggatc agtcaaagcg caagaaatca ttcgatttag agaagaaaac ggatccttcc
    43561 agaaagtgga agacctgcag gagatttctg gtatcggtga aaagacagtt gaaaaactaa
    43621 aaaacttcgt tacaattaaa tagaatgatt caaaatcgac tggcacgagc aataaatgga
    43681 cgctaatgga tactaagaat aatccaattc taagagcttg gcaggacgag gaggtagtat
    43741 ttttggatag gaaatagctt gattgttcgc aaagaaacat taaaaaaacc aatgctgaat
    43801 gtctatctcc aaaataaaat atctgggatt catattatga atacggctgt cagcggcaat
    43861 aacagtcaag ctctacgaga acgctttgcc aaagatgtac ttagctatac agcagataaa
    43921 gtatttattc tcattgggac gaatgatcta gcagagcata aacaactttc taaagaaacg
    43981 taccaaaaaa tctgcagtgg atgatagttc agctgaaaaa agactatagt tctgatcatg
    44041 ctttctttat cacgccacca gcagtcgatg agacaaaaca aaaatatcgg aataacaagt
    44101 tgatcaaaga atatatctct gttctcacag aagtagcaaa acgccatgat tgtgaagtat
    44161 cgatttttat acggaatttc ttgaacaaat ggattcgaag aatttttata atgggctaat
    44221 ggatgacgga cttcatttcg gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg
    44281 ttatatttaa agaatatttt gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg
    44341 gaggaatata aaacatgtta gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag
    44401 ttactgataa gtcacataat gaacgcattc cttgggacca gtactttatg gctcaggcag
    44461 tactgttatc tttacgtagt acgtgtacac gtttagaagt aggagcgaca ctcgttcgag
    44521 aaaaaaggat cattgctggc ggctataatg gagcagtcag tggagatgtc cactgcatag
    44581 atgaagggtg ttacgtcgtg gatggacact gcttgcgaac gatccatgca gagatgaatg
    44641 cactcctaca gtgtgcaaaa cttggtatcc cgacggaagg ttcagaaatc tatgtcacac
    44701 attttccatg tttggcctgt acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt
    44761 atctccatga ctatcgaaat gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat
    44821 ctgtacacca agttgcttta gactcacagc atttttcaaa acttcaaaat gaattaacag
    44881 gaaaacatgt ctagcgtttt agaaaggcat gttcgaaatc ggttgatttt tccggcaatt
    44941 acgtcgatga ttatctgttt gttgtattac actgacaatt ggggttatcg tttgcttctt
    45001 gccggaattt ttttatggtt ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt
    45061 ttgtgtagca taacggtatc catttcttat aggctacaag agtattcatt tttagaggaa
    45121 aaaacaacat ctgcgcaaat tactgtattt acagatacga ttcgtgcaaa tggtaatttt
    45181 ttgacaatgg aaggaaggtt atcagagaat aaacaaaagc tccagctttc ttatcaagta
    45241 aacacagaag cagaattgaa aacatggctt aacttccgag gaaagggaat agaattgact
    45301 gctgaaggcg agttcatctc tccagcaaaa aagcggaatc tttataattt tgaccaagaa
    45361 gcttacttca ggaaccatag aatttctgga cggttccaaa ttcaaaagat tgaacagatc
    45421 accatgcaaa atcactggcg aaactggctt cagagaaaaa gaggatcttt tatctccttt
    45481 gtccaatctg aatttccgca aaaaacaagt gtatatatta attctttatt atggggctat
    45541 aaagatgcag gattcagaga accagaagaa atttttaggg agagtggttt gctgcatctg
    45601 ttcagtctat cgggtctaca catccaattt tatttagggt ggctgtatta tttgtttcga
    45661 cgtatcggat ggccactaca cgtcagtatt attccgttga gtctactaac tatctgttat
    45721 gtttctattg caggaagttc tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga
    45781 ttgctagtca aattgctgaa taagcattat tccaccatgg acttatttgc agttgtcttt
    45841 tggcttcttt tactatttga tcctcgattt ctttttcatg cgggtggaca gcttagtttt
    45901 ttcatgagtt ttttgtttat cctgatttct tttgatggag attggttgaa aaaaacggcc
    45961 tctcacgtcc tgtttactat gatcacgatg ccgttgattg tatggcattt ttatgagtgg
    46021 cctattttag gtagtctttt tacattactg attgcaccgg tattcaagtt tctttttctt
    46081 cctggtgtgt tatttctcac actcttcaat cgctggctgc cggaaacttt acttttgctg
    46141 atagaagagg gattaatctt atttgagcga ataatcgatt tttcttcagg gtcgtctctc
    46201 attataggac gtttgcccct tgtgctatct gggatgtata ttttcggatt actgatttgt
    46261 atggataggt tgaaagaaca acctgtgaaa cacttgtttt atgttggtct tttttctttt
    46321 atttttttga ctttgccatt tttacgcttt tcttcaacga tcgcttttat cgatgtcggt
    46381 caaggagaca gtatcttttt gcaaagtcct tttcgaaaag aaacgatttt gatcgatact
    46441 ggaggtcagt tacactttga aagagaaaag tgggcgcaag gtatgatacg tcctcctgct
    46501 gaaagaaata tcgttccata tctaaaaggt caggggataa gtgtaatcga taaactcatc
    46561 ttgacacacg acgatacgga tcatgtggga gaattaccca cgttatctca gcattttact
    46621 atcaaaaaaa tttatatcgg ttggggcgct ggacgaaatg agccattaca aaggcattta
    46681 tctgaagctc aaaaaaatgg tactgagatc attgaaatca aacatgggga caggatcagc
    46741 ggttattatg atctttatgt attgactcct tttgaaaagg gagagggaag aaatgaagat
    46801 tcgattggtc tatggatgga atataacaat aggcgctttt tatttttagg agatttgaat
    46861 caagcaatgg aaaaacaatt gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa
    46921 ttaggtcatc acggtagccg tacgtcaagt aacacagatt ttctttccca tattgaagca
    46981 aaacatggga ttatatcctg cggcgtaaat aatcgttatg gtcacccgca cagcgaagta
    47041 ttggagacac tagaagaaaa tcagataaaa acatggcgaa cggatcaaca aggaatgatc
    47101 acctatcgct ggcatccagt ttttcatcct catgggctag tggaaacaat gatagactag
    47161 taattctcat ctgctcatgg taggataatt tttgaaagtg gtgagtaatt tgagtataca
    47221 agcagagtta caaaaaatac gtaaagaaaa acttgcatct tgttacttga ttttaggaac
    47281 agaaaagttt ctccaagatc aagttcggac agaaattgtc aaaaaaatcc aattagaggg
    47341 cgaagatgac ttaaattttt taagttttga catggaaaat gcaagtttag atgaggtagt
    47401 agctgaagca gagacgttgc cgtttttcgg agatcaacgg ttagtattcg tagagaatcc
    47461 ttatttttta acaggtgaaa aagtaaacaa tggaatcgaa caaaatactg atttattgac
    47521 ggcttatttg aaagaaccat tagaatcgac tgtattagta ttctttgcgc catatgaaaa
    47581 gttggatgaa cggaaaaaag taacgaaaca attgaaaaaa acagcaatta tcatcaatgt
    47641 ccaacagttg aatgaaaaag aagtacgtca atatctgatg aataccttag agaattcctc
    47701 aatcaaaatg gaacgaagtg caattgattt attcttgagg ttgaccgact tagatttatc
    47761 caaaatgatg agagagttac agaaattggt cctttatgga caaaatcaac agcaaatcac
    47821 ggtaagagaa gtagaagaat tagttcctaa aacgttagaa cataatattt ttgacatgac
    47881 ccaatatatt ttaaaaggga agacggaaca agctttgcgt ttatacgaag atttagttat
    47941 gcaaggggaa gaaacaatca aaattaatgc gattttgctt tctcaattac gtttattttt
    48001 acagacaaaa tttcttgtaa agattggtta tcagcaggca aatattgctg aaacattgaa
    48061 aatccatcca tatcgtgtaa aacttgccat gcaggaagta aaaaaattcg atgaacattt
    48121 gctggtacag ttgtttgatc aattagttga gatggattat caaatcaaaa cgggacaaat
    48181 cgaaaaagaa ttaagttttc aactattcgt tttgcgcaca gggcagttgt taagaaatcg
    48241 ataagaagaa agacgagggc aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg
    48301 acaaataaaa aaatcggccg aagccgattc ttttatttag cgattttttt gcttaaacga
    48361 gatttgtcgc ggttagcttt gtttttgtgg attagacctt ttgattcagc catatcaata
    48421 gctttaacag cttctttata taacgcatct acgttgtcag cacctgaagc tacagcatct
    48481 tcaaattttt tgattgcagt acgcatagcg cttgtttgag atgaattttt cacattagcg
    48541 ttttcgcttg tacgaacacg tttgattgca gattcaatgt ttggcattag tttcacctcc
    48601 gataacttaa gttcttacgc gtgttaccgc ataattcaac attcattatt atacctaaag
    48661 gcagcgtgct ttgcaatatg aacatgacaa gtttttttcc ttaacagtct tttattagga
    48721 ttttttttca gtaaaaacgg atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg
    48781 gaggcaatag cgtcaaacca aactagattt ttatttatct atttaaacaa agtttgaatg
    48841 ggaatgtttg gtactaagtt attatataac aaagagaacc gtttgcaaag ttttttttga
    48901 aaagggatac gattccctgt tgttctttgt ttttctatta agaaactgca gttattttat
    48961 aaccagtatc ctttgatcag taaaggctat tctggtgtac cgattatttt aatgtattca
    49021 tcgatatttt ttatcaacat tttttttatg accaactggt tccgacggtt ccatttgcca
    49081 cttacttcca gcacatattt atttaggggc aaacgtaaga tatattttgc caaatagcga
    49141 tctttaagca ggccattgat cacacggtcc tcaagctgca agttgatccc agtcagcatc
    49201 attggctgtt cttgtaaaac gtacgtatca attaaaagac cttcaaaagt ttccacaagc
    49261 gtcttccttt cttttcaaag taacctgaaa acaggcacta attcttccat tttgatattc
    49321 gttgaagtgg atgcgatcat tctttctttt gtccactgta atctagtata acaaaaaaga
    49381 gcagaagagc caaaaagaaa aaagagcatc gaattctata ggaagtctca ttggcacggc
    49441 ctaaattatt acaaaacatt gattaaaaaa agcccctgca tcgcaggagc atcccctata
    49501 tcgttttaat aggtcaatca aaaagcagta tcaaacacat tttgtatcaa atcaggaaat
    49561 aatagataag ctaagcccag aatcaaaagt accgctagaa gaaaccagcc gattgttcct
    49621 atgaaatact ttaatttttc gaacattgta tcacctcgtt gtcagtatat cacggatcag
    49681 taaattgggg gtgttaagaa tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc
    49741 aaatacttgt aagatacagt aagtgatacc agaagcaatg accggtccgg cggcgattcc
    49801 tcttaagaag acaacaccta aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc
    49861 agaagcaatc aatcctacac ccttggaaga taagacagcc actagcccac cgcaaaaaac
    49921 agctacccag ccaatagggg atttaaaggc ttctaataga tcacgtagac cgatttctcc
    49981 tgttgcgata gggattagaa tagccgctgt gatgatcgtc accccaatat tgattccttt
    50041 gccttgaagc caagggaaaa aggaacggga ggctggtatg agcttcataa tcaaaacaaa
    50101 agctgccgct atcatcagac tttgattttt agctaaaatt gctacaagta aaataattcc
    50161 taaaaaaagc caactttcca tgaaatatat tcctcacttt cttcgaaaca atcataacat
    50221 gtttttagaa agttacaaaa gtaggaattc atccttcaag acctattcat aagctgtttt
    50281 tttcataaaa taaacaaaat cataaaaaaa cactttgctt atttgacaga agacagccgc
    50341 gctttgtata gtggacaaga cttacatttt tttgaagaat cagaaagatc aggtaaaggt
    50401 cttagtaaac gcaagccatt cagaataacc aatattgtgc ttccttcgtg tcctacaact
    50461 ccaagaggta gatttaaaaa ttgcaggaag ttagaagcaa tcaacaagag aataacaaga
    50521 atcgaaaaaa taatattttg tttgatgatc cgccgcattt ttaaagatag ccgatgactg
    50581 tagacaaatt tatttaagtc gcttttcatt aagacgatgt ctgcgatatc catagcggca
    50641 tctgtccctt gccccatagc gacacctaaa gaagcggctg ctaaagcagg cgcatcattg
    50701 actccatcac caaccatcgc atttacttgg taaagttcat tctctttttt gactagttgt
    50761 gttttttctt ccggagtaca gcttgcatag taatgttcaa tccctacttg ttcagcaatg
    50821 gcctttgctg tgccagaatg gtcgccggtt atcatacttg tatggatgtt ttgggattta
    50881 aagtaggaaa ttacttgttg ggtatttgct tttggaatat ctagcagacc taaaacagca
    50941 attaactgct gatctttaga aagataaatc acggttttgc cttgattttc caaacgttcg
    51001 atcatttccg caatctcttt gctgattatc atcgtctcag gatcatatgc gtgcttgcca
    51061 actttccatt gcgcacctaa ataggtagtt tgtaatccaa aaccagtaat ttcttctatt
    51121 ggtaagtatt ggatttcttc aggtattgtg caatcgaaat attgcaaaat cgcatgagca
    51181 agtggatgcg tcgttttttg ttccatagca acaagaagtt gttgtgccaa atccttttca
    51241 tcaaagaaaa agctgtcagt tacaactggt tttcctttag tgagcgttcc tgttttatca
    51301 aaggcaatcg cttttaattc agaaagcttt tctaaatgga taccgccttt gattaaaatc
    51361 ccattttttg cagaatttga aagagcagct agagaagctg gtgtagcaga tgcgacaagc
    51421 gcacaaggag aagccacaac taataatacc attcctcgat aaaagctttc atcccaactc
    51481 caacctagga aaaagtgggg gagaaggatc attagaggaa cagcaagcaa aatcaatttt
    51541 acataattat tttcgattcg ttcgataaat gaagctgttt gagttggcgt attctgcgct
    51601 tcttctacta attggataat cttagcaaag acagtttcgg aactcgtttt tgttactcga
    51661 atcgtgatgg gatttccttg attcagcgtt cctgcataca tctcatcgcc taaatatttt
    51721 tctacaggca cagattcacc gcttaatgca gactcatcga tcgttgcctg tgtgctttct
    51781 aaaaagccat ctatcggaac agctgctcct ttagcgacaa ataccaagtt atccaccttt
    51841 agttgagaaa catctacttc ttgcatttgt ccattttctt ttaataattg ggctttttgt
    51901 ggttgaagat tcatcaaagc agtaatttct ttttgacttt tattagttgt gtattcctct
    51961 aatgcaccgc ttaaacaaaa gatgaatgtc aacattgctc cttcaaagta attaccgatc
    52021 aaacaggcac caatagcagc taaagccatc aataaatcca cattcagtgt ccgatcattc
    52081 cacaattctt tcaagccttc cgatgtttgt ttatatccac cgatgataat ggcactgata
    52141 taaaaaagtg gggtgatggt aagagagaat cggtcgaaga taaaaccgaa gatcataaat
    52201 aaaaggcagc cgattgttgc tgcaaaagct tttttctgat tagtcatcgt tattagacct
    52261 ccttgtcaaa acaaggatag cataataaaa atgtaattga taataataac agctaaatga
    52321 gaacgataat ggttatcatt tggtgattaa gcttgatgtt gtttttatta tttataatga
    52381 ttattattaa agaaaataaa aaaccagaac atttctcaaa taagagaagt gttctggttt
    52441 tttctacatt gcaataaacc aataaccaat caaaatgata ccaaggataa tacgatacca
    52501 gccaaaagcg gtaaagtcgt ttttcttcaa atagcttagt aagaatttga tagctaagac
    52561 ggaaaccaca aaagatacga atgttcctac aagaagaaca acagtttctt ccattttgaa
    52621 tgaattccct tcagctaaat atttgacgat cttcaagccg cttgcgccaa acatgacagg
    52681 aatccctaag aaaaatgaaa actctgtagc gacaaaacgc gaagcaccaa tcaagataga
    52741 tcctaggatc gttgcgccag aacgggatgt tccgggaaca agtgccaaga cttggaataa
    52801 cccaacaaga agagctgctt ggtacgtaaa gtcattcaga gaagtccaac gaggtgtttt
    52861 gtttttgttg cgtttttcga caataataaa ggcaataccg taaacgatca aaacaatggc
    52921 aactggtaag aacttgtgga aatttgcttc taaccaatca tccaaaggca aaccaatgat
    52981 gatggctggt atgcaagcaa cgatcacttt aaaccaaagt gtccacgtat cttttttttc
    53041 taccgttgat ttacttggag aaaatgggtt taacttgtga aaataaagaa cgacgacagc
    53101 caagatcgcc cctaattgga taacgacatt gaacatcgac ataaattgtg aactggcatc
    53161 cagtttgatg aattcatctg ctaggatcaa atgaccagta ctactgatgg gcagccattc
    53221 cgttattcct tcaataatac cgagaatcac tgctttgata atatttgcaa aaaacataaa
    53281 ttccttcctt cccaattaag atacatctta tagtataccg ttattttttg agaaacccaa
    53341 tctgtttttt aatattaaga aaaacttgag aaataaagca gataagtaca cgaagagaaa
    53401 aaagaagaca aagccctcgt tcagactttg ccttttatga ttctctggta aaaattaaaa
    53461 tatccccatt atagaaacga gtcgcgacta tgtgttttcc cgttatcgat ataagacgta
    53521 tcattgatgc cgtggataga aaatcgaccg tttgcataag tgacctttgt tacgctgcca
    53581 tttaaaagcc cagcacgaac aggctgttta ggatcgatca gattaagcaa aaaggcgatc
    53641 gtcaaaccat gagatacgac taaaacattt ccaccaccat ttttttcgca gtgatgggcg
    53701 atatcttcaa atcctgtcca tacacggtct cgcaatgttt cataagtttc cgcccaccca
    53761 gcggtatcta agttgtatac agcattagca atttgttcaa aagaaacttc tgtggtaaac
    53821 atttcatcat atgttttaaa attcaataca cgagggatca caccccacat ttccatgtca
    53881 tatccacctt caagagaccc gaaacaccat tctctaatcc ttttttcaat aaaataagga
    53941 atttcttttc ccttagaatg ttctcctaga ataatacggg cagtttcgat tgcacgacca
    54001 ctgtcactag aataagcaaa tgaaaagtcg acatttttta acccgcgacc taaataatga
    54061 atgccttctt gtccttgctt tgtgaggggt gtatcacacc agccttgtac tcgttcaatt
    54121 gtattgaaca tcgtttttcc gtgtcgtacg atataaagtg tagtttctgt cattttattc
    54181 tcctcctaag tcgttttaac gattgaaaaa aggatttgtc cttttttcct gttcaatcgt
    54241 cgttgcatcg ccatgtcctg gatagactgc aaattcatca ggtaatacaa acagctgggt
    54301 ccgaatacta tgaagtaatt gttctaaatt ccctgtatac agatcggttc taccgatact
    54361 tcctctaaac agggcgtcgc cagaaacgac aaaatcatca aaaataaagc tgacgcttcc
    54421 tatagaatgc cctggggtag ggacgacgcg aaaagacatg tttcctaaag tataagtttt
    54481 catttcaaat tcatattctg caggcgagac aataacatca gcaatatcat catgtctacc
    54541 gagtccagaa agattcaaaa tcggatcacc tagccatttt tgctctagtg ggctgacata
    54601 aactgggatt tgataatacg cgcgtaaagg ttccactgca ccgatatgat cgtagtgagt
    54661 atgggtaagc aaaatcgcta ccggctgttg gtttgttttt ttgatttctt gtttgattcg
    54721 ttccgcatct tctccagggt caatgatcaa aagtgcttct tcgttgtaga ccaaataaca
    54781 gttttcttca atcattcctg tttttaatct ttcgatatgc atgtgcattc ctccttgata
    54841 gccaaaactt ctttcatcca ttagtataac gcattcattg ctaggaaaca aaaacaggta
    54901 cttatttctc tttacttatc tgaattatca gactatatga aaagtatttg gatattctaa
    54961 agagaaggaa agtaagtttt catagcattt taatatgtcc ttgttacatt agattcatcc
    55021 aaaaatccaa attctatatc ctatatccct gcctaatcag cagggtgttt ttttatgttt
    55081 ggaaagagag ctctttttgc taaactaggt tttttcaggt aatcttagag agaaagaggt
    55141 ggtggaaatg atcaaacagt caaaaatatc tgtgcggcac ttagtagaat ttattttgcg
    55201 acgtggaagt atcgacaatc gtaaaaaaag taaccatact gcattagaag gagcgagaat
    55261 ccaccgcaag ctccaaaaag atgcgggaga aaactatgaa aaagaagtct ttttaaaaac
    55321 aacagttcaa ttagatgaat attacctgat cgttgaaggc cgtgcagacg gtattttcaa
    55381 aagagacggt atttattata tagatgaaat caaaacctcc gaaccacgat ttgaagattt
    55441 agaaccagaa caagtggaac ttttctttca tcaagcaagg gtgtatgcct atatctatag
    55501 tcaagaacat gatttacaag aaataaatct gcaattgact tacttccaga cggcagaaga
    55561 agtgatcacg agaaaggttg aacatcagac aagagagcag ttagaaactt tttttaaaaa
    55621 gctgactgaa gattataaag aatggcttgt ttttcaagaa aactggcgca ctgtacgcaa
    55681 tgcctctttg atggctttga aattccccca tgatgagtat aggaaaggtc agcgtgaact
    55741 agcggtagct gcttataaaa cgatccgtac gaaacagaaa ttgtttgtag aagcacctac
    55801 cggaacagga aaaacaatct ctacgttatt ccccgctttg aaagctgtag gggaagaaga
    55861 gggagaaaag atcttttatc taacggctaa aacaataact agacaagtag ctgaagatgc
    55921 tatgacagca ttaaaagata caggagctga agtaaaaagt gtcactttga cggcgaaaga
    55981 caagatctgc tttttagatg agacgacctg taatccagat cagtgcccat atgccaatgg
    56041 ttattacaat cggattaatg aaggtttatg ggatcttttg aatcatgaga atcagatcac
    56101 tcgtgaagtc atcgaaactt atgcaagaaa gcatacactt tgtcctttcg aactatcatt
    56161 agatgtcagt atctggtgtg acgtaatcat tggggattac aattatttat ttgatccaac
    56221 tgtttattta agacgttttt ttgaagatga aaaaaacgag gactatctgt ttttgattga
    56281 cgaagcacat aatttggtga atcgttcccg agagatgtat tcggcagaac tatcttatga
    56341 aaaaacgaaa cgaagtaagg aagctatacc aaaggaattc aaaaaacttc atcgccgttt
    56401 caacaaacta ttaaaagaat ttgattcgat ccgagaaata gcaaaagaag atcattggga
    56461 ttatcatcat caaaaagcac cggctgaatc tttagtaaag gcaggttatc aattaagcga
    56521 aaaaattaaa gaatggctgg cagaatttcc tgagcaccca tcacaagaac aattgcttcc
    56581 ttattatttt gatttgcttc attttttgaa ggtcagtgaa ttttatgatg atcactacga
    56641 aacaacagtc gaaaaaacgt atcgcgatct gatagtaaaa gagttttgta ttgatccaag
    56701 cttgtttctt gagcagtcgt tggataaagg aaaaagcagt attctttttt ctgctagttt
    56761 ttcaccactc tcttattatc aggaaacact ggggggacaa aaaagtctgg cttacaaact
    56821 gccgagtcct ttcccagaag gaaaccagca agttttcatc gcaaattacc tagagacgac
    56881 ttatcgaaaa agggaacaca gtctggcaaa attagtagag acgattcatg cttttgctga
    56941 tggaaaggtc gggaattatt tcgttttctt tccttcttat caatatttag atcttgcggt
    57001 ggaagcattc agacagcaat atcccgacag caatgtgcta atccaagaaa cggacatgaa
    57061 tgaggaagaa cgcgaacgat ttttaacaaa atttcaaaca aatcccgctg aaacattgat
    57121 tggattttgc gtacttggcg ggattttttc agaaggaatc gatttgagag gggaccgatt
    57181 gatcggcagt atgatcgtag gtgtaggttt gccgcagatg aatcatgagc aggaactgat
    57241 caaatcatat tatgatgaga aggaacacct tggatttgct tacgcttatc agcttcctgg
    57301 aatgaacaaa gtcttgcaag ctggtggtcg agtgattcgt ggcatggcgg atcaaggaat
    57361 cgttgtattg gcagatcaac gattttctag tttttcttat cgacgtttat tcccacaaca
    57421 ttggcaaaca gcaagagaag tacgaagtgt tggggaattg gaagaaggaa tacagcgttt
    57481 ttggttatca aaagaagcag taaatcaaaa aaacgaggaa tgacagacgt cagcctcgtt
    57541 ttttgattaa cgatagttag tgaattgcaa ctcgattggt aagtcaagtt tacgtaataa
    57601 agcaatcaca gcttgtagat catcacggtt cttacctgtc actctaatct gatcttcttg
    57661 gatttgtgct tttactttca gtttttcatt tttgatagct gtagtgattt tcttcgcgtt
    57721 ttcacgatcg attccgttga ccaaatcggc agtttgtcga gctttgccac ctagagcgtg
    57781 ttctgattcg gaaaaatgga tatttttgat tggcacattt cttttgatca gcttaccaaa
    57841 taatacatct ttgatctgtt cgattttgaa atcatcatcg ctcaatacaa ctagattgct
    57901 gttctctaat ttgatttcta ctgtagaatc cttgaagtca aagcgattgg acaattcttt
    57961 tgttgtttgc tgaatcgcat tttttacttc ttctgtattg acttctgata caatgtcgaa
    58021 gctagcatct tttgccatag gaaccctcct gatatacata taaatattta ccatacatct
    58081 atcttatcat aggattaggg cagattctta cctgtttgct aaaattcttc tcatttgagt
    58141 tcgaagtatt ttattttaat agataaactg ttattcttac atttggaata ccaaagtagg
    58201 ggagaggtgc ttgtgagtaa aattatgact gcgtatcata aaaatgaaag attaaagaaa
    58261 atagtggtta ttttgataac cggattgacc gctgcggtcg ctttaaatta ttttttgatt
    58321 ccagccaatg tcttttctgc agggatgaat gggatcgctc aaatcatttc ctcgctttta
    58381 tatgaatggt ttcatatcaa gatagacaca ggaacattta tttttctttt gaatatccct
    58441 gtgtttgtat tagggtttct caaagtagga aaacgagcaa cgatccttag ttttgtgaat
    58501 gttgtatgtg tatcggtgat gacgatgctg cttccagtcg gacagatcac tgataatatt
    58561 ttgatgaatg ccctagtagg cggtgtttta ttaggtcttg gcgtaggatt atctttaaaa
    58621 atgggcttta caactggtgg gatggatatc atctctcttg tactttcaca aacaactgga
    58681 aaaacagtcg gaaactacat gctgattcta aatggtatca ttgtagtcat cgcaggcttc
    58741 ttctttaact gggaaagtgc cttgtatacg attatttcca tttatgcaat gactcaagta
    58801 attgatgcca tccacacgag tcatcaaaaa gtaacagcaa tgatcgttac cgtcaagcca
    58861 gaagaggtaa cagaagaact agcaaaacgg atggtacgtg gaatgacatt actgccgtct
    58921 gtcggtggct attcaaaaaa acaagggaac atgatcatga tggtcatcac ccgctacgaa
    58981 atgtatgatc ttgatcagat cgttcatgaa atcgacgacg atgcatttat caatatttta
    59041 ccaactcatt cagtatttgg ccggtttgca aatgaaaacg aacaaaaaat gtatcgaaca
    59101 actggcgtat tgccagaaat aaaaaatcgt tcacaaaaaa aatgaggaat caggtttatt
    59161 ttagaataaa tttaatctat tacatctaat aagaacattt ttataaattt aaacgagaat
    59221 cttacttcta aattctcatt tacaagcagc taaaaaaaga gtatgataaa taaaaatcgt
    59281 gttaaggagt gtcgtacgat gagcgaagca aaccaagaac ggtctggaca agaaatagaa
    59341 ctaatcaaag aacgtattct tgctgccttg gaaatggtga ttgatcctga attaggaatc
    59401 gacatcgtca atttaggctt gatttatgat atagaattca atcctgaaaa tggagaaaca
    59461 gtcattaaga tgactctaac aactatgggc tgtccattag cagatatttt aacagaatct
    59521 atccatgacg cattaaaaga agtgccagaa gtaacgaaag cagaagtcaa actagtctgg
    59581 tatccagcat ggacgacaga taagatgagc cgttatgcac ggattgcgtt ggggatacga
    59641 tagaaatctt ggctgactag ctatgagaag caaataggaa aaacaaaaaa gccttttatc
    59701 ctttctagaa gaaaagagat aaaaggtttc tttgtttata aaattttgtc tgctatatta
    59761 taagttaatt aaataaatgt gttctattga taaaggacat tcaaatccct ttccttttgg
    59821 aatccttctt ttttacatca gtttgatttt cgtatagtat tctttctcat cttcacgata
    59881 aatcgcagcc ccattttttt gttgtgcttt aagactgcca agattttttt tatttacaga
    59941 caaaagtgcc tcatcaatgc cgattttttt tgcctcttgt agtactaatg ctaatccttg
    60001 tgtggcatat ccttttttac gataattagg ggagatgccg taaccaatat ggccagcgcc
    60061 gtttcttaat gtttcgttca gtttatggcg caaattaaaa attcctacat actcatgttc
    60121 atcactttct aaaataaaga cggttgcggg aacgtaactc tcaggaagat cgattcctaa
    60181 agacgttttt tcgcattttt ttacataatc taaaaattca gcaaatgtat agccataagc
    60241 attgttgata aatccactct catttggtcc aaagctagtt tgtagctgat aagctttctt
    60301 taaatctgct tcccatagtt tgatcagttt catcgtattc acctctttaa gtaaccttat
    60361 tcatttgttt tctactatag cactattgtt gtaaatagcc atgcctattt ttatttattg
    60421 tgtcatatcg tattaagaat catcgaacaa tacaaatatg aattacaata aaaaaattat
    60481 ttgaagttcg ttcattcgtt acttatgatc tctagaagaa agaaaacgtg aattacttca
    60541 gcgtctagaa ttccagaagg atagtaatca acagaagaaa taatcaatat ccaaatgaaa
    60601 gaagatagct gaatcgagaa aaagaggaaa aaagaaatga agaaaaaaat aaaatagtta
    60661 ctaatcccag agtcgttcgt ttaacattga aatcgatcat atggataaaa cagatttttt
    60721 caccaaccct caatagatga tggagttgct cggatgaatt atttgtgtag atggcataag
    60781 ggagggcgta cagaaatgaa aaagaacaaa aatatgttct tgacaaaaaa cacatgtaat
    60841 cctaaactaa aagatgtgat gcggctatgg cggaattggc agacgcgcta gcttcaggcg
    60901 ctagtgaggg tgacctcgtg gaagttcgag tcttcttagc cgcataattt gtttttttat
    60961 aaagagaagg tacacctgga gcgatgagaa agaaaataac atattatcta aaagaatata
    61021 ggaaaaattt taaacaacgg aaccagattc aaataaatca aaaaaaattt gtgtgaaggt
    61081 gggacagttt aaaacctaat agattgattc aaatagtaga tttatagatt ctgatacaag
    61141 aaatactggc aaacgatagt taaggaataa agactaaact taaactcaaa aagaggtcat
    61201 gcgaaagaat aaatcgcatg acctcttact atattttgaa tgtgtttaaa cgttcaaata
    61261 atgaatctta gtaccaaccg tttgcttccc agaatgcttt cgccgcttcc caagaaccat
    61321 aacgtgaagt aacatattgt tctgctactc tttcttggtt tgcagcagag tagtcaccat
    61381 ttaagtaaga agaatctaat tggtaacggc cgatgtaacg tccgttcgtt gctgtgtaag
    61441 aaccgcttga ttctttttgt gcgatccatt cttttgcaga gcttgtactt gctggagtag
    61501 cagcagtttc tgttacagca ggagcaggtt gtgtttcagc aacaggcgct gcttgtgttt
    61561 ccgtttgaac cgtttcttgt acaacaggtt gttcagcttg aacaggttca gcttggactg
    61621 gtgcttcttg aacggctgct tgaggttcag cagctgcttg tgcagagcct tctgttggga
    61681 ttgttaattg ttgtcctgag ta
//
LOCUS       DADVXU010000006        56779 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.006, whole genome shotgun sequence.
ACCESSION   DADVXU010000006 DADVXU010000000
VERSION     DADVXU010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 56779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.006"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            52..1683
                     /locus_tag="KB953_000365"
     CDS             52..1683
                     /locus_tag="KB953_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HBB6865368.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQFPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(1745..2386)
                     /locus_tag="KB953_000366"
     CDS             complement(1745..2386)
                     /locus_tag="KB953_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBB6865369.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    2572..2648
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            2668..2976
                     /gene="rplU"
                     /locus_tag="KB953_000367"
     CDS             2668..2976
                     /gene="rplU"
                     /locus_tag="KB953_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBB6865370.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            3003..3347
                     /locus_tag="KB953_000368"
     CDS             3003..3347
                     /locus_tag="KB953_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HBB6865371.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            3360..3653
                     /gene="rpmA"
                     /locus_tag="KB953_000369"
     CDS             3360..3653
                     /gene="rpmA"
                     /locus_tag="KB953_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBB6865372.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            4087..6894
                     /locus_tag="KB953_000370"
     CDS             4087..6894
                     /locus_tag="KB953_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBB6865373.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            7085..7576
                     /locus_tag="KB953_000371"
     CDS             7085..7576
                     /locus_tag="KB953_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBB6865374.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            7679..8668
                     /gene="manX"
                     /locus_tag="KB953_000372"
     CDS             7679..8668
                     /gene="manX"
                     /locus_tag="KB953_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HBB6865375.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            8701..9504
                     /locus_tag="KB953_000373"
     CDS             8701..9504
                     /locus_tag="KB953_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HBB6865376.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            9525..10439
                     /locus_tag="KB953_000374"
     CDS             9525..10439
                     /locus_tag="KB953_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HBB6865377.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            10522..10896
                     /locus_tag="KB953_000375"
     CDS             10522..10896
                     /locus_tag="KB953_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HBB6865378.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            11035..11280
                     /locus_tag="KB953_000376"
     CDS             11035..11280
                     /locus_tag="KB953_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBB6865379.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            11452..12516
                     /locus_tag="KB953_000377"
     CDS             11452..12516
                     /locus_tag="KB953_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBB6865380.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            12762..13187
                     /locus_tag="KB953_000378"
     CDS             12762..13187
                     /locus_tag="KB953_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBB6865381.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            13180..13629
                     /gene="nusB"
                     /locus_tag="KB953_000379"
     CDS             13180..13629
                     /gene="nusB"
                     /locus_tag="KB953_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBB6865382.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            13726..14580
                     /locus_tag="KB953_000380"
     CDS             13726..14580
                     /locus_tag="KB953_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HBB6865383.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            14581..15918
                     /locus_tag="KB953_000381"
     CDS             14581..15918
                     /locus_tag="KB953_000381"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBB6865384.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            15923..16147
                     /locus_tag="KB953_000382"
     CDS             15923..16147
                     /locus_tag="KB953_000382"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBB6865385.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            16149..17039
                     /locus_tag="KB953_000383"
     CDS             16149..17039
                     /locus_tag="KB953_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBB6865386.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            17048..17866
                     /locus_tag="KB953_000384"
     CDS             17048..17866
                     /locus_tag="KB953_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HBB6865387.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            17973..18422
                     /locus_tag="KB953_000385"
     CDS             17973..18422
                     /locus_tag="KB953_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HBB6865388.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            18440..20122
                     /gene="recN"
                     /locus_tag="KB953_000386"
     CDS             18440..20122
                     /gene="recN"
                     /locus_tag="KB953_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBB6865389.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(20177..20293)
                     /locus_tag="KB953_000387"
     CDS             complement(20177..20293)
                     /locus_tag="KB953_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HBB6865390.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(20366..21322)
                     /locus_tag="KB953_000388"
     CDS             complement(20366..21322)
                     /locus_tag="KB953_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBB6865391.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            21493..21861
                     /locus_tag="KB953_000389"
     CDS             21493..21861
                     /locus_tag="KB953_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HBB6865392.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            22031..22462
                     /gene="mraZ"
                     /locus_tag="KB953_000390"
     CDS             22031..22462
                     /gene="mraZ"
                     /locus_tag="KB953_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HBB6865393.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            22483..23442
                     /gene="rsmH"
                     /locus_tag="KB953_000391"
     CDS             22483..23442
                     /gene="rsmH"
                     /locus_tag="KB953_000391"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBB6865394.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            23447..23848
                     /gene="ftsL"
                     /locus_tag="KB953_000392"
     CDS             23447..23848
                     /gene="ftsL"
                     /locus_tag="KB953_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HBB6865395.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            23849..26041
                     /locus_tag="KB953_000393"
     CDS             23849..26041
                     /locus_tag="KB953_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBB6865396.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            26067..27029
                     /locus_tag="KB953_000394"
     CDS             26067..27029
                     /locus_tag="KB953_000394"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBB6865397.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            27043..28404
                     /locus_tag="KB953_000395"
     CDS             27043..28404
                     /locus_tag="KB953_000395"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBB6865398.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            28430..29518
                     /gene="murG"
                     /locus_tag="KB953_000396"
     CDS             28430..29518
                     /gene="murG"
                     /locus_tag="KB953_000396"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBB6865399.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            29533..30753
                     /locus_tag="KB953_000397"
     CDS             29533..30753
                     /locus_tag="KB953_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HBB6865400.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            30909..32234
                     /gene="ftsA"
                     /locus_tag="KB953_000398"
     CDS             30909..32234
                     /gene="ftsA"
                     /locus_tag="KB953_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HBB6865401.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            32257..33498
                     /gene="ftsZ"
                     /locus_tag="KB953_000399"
     CDS             32257..33498
                     /gene="ftsZ"
                     /locus_tag="KB953_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBB6865402.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            33515..34192
                     /locus_tag="KB953_000400"
     CDS             33515..34192
                     /locus_tag="KB953_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HBB6865403.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            34206..34805
                     /locus_tag="KB953_000401"
     CDS             34206..34805
                     /locus_tag="KB953_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HBB6865404.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            34820..35107
                     /locus_tag="KB953_000402"
     CDS             34820..35107
                     /locus_tag="KB953_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HBB6865405.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            35156..35938
                     /locus_tag="KB953_000403"
     CDS             35156..35938
                     /locus_tag="KB953_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HBB6865406.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            35959..36666
                     /locus_tag="KB953_000404"
     CDS             35959..36666
                     /locus_tag="KB953_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HBB6865407.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            36928..39726
                     /gene="ileS"
                     /locus_tag="KB953_000405"
     CDS             36928..39726
                     /gene="ileS"
                     /locus_tag="KB953_000405"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBB6865408.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(39775..41301)
                     /locus_tag="KB953_000406"
     CDS             complement(39775..41301)
                     /locus_tag="KB953_000406"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HBB6865409.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(41316..41963)
                     /locus_tag="KB953_000407"
     CDS             complement(41316..41963)
                     /locus_tag="KB953_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HBB6865410.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(42147..42476)
                     /locus_tag="KB953_000408"
     CDS             complement(42147..42476)
                     /locus_tag="KB953_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HBB6865411.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            42653..43381
                     /gene="sfsA"
                     /locus_tag="KB953_000409"
     CDS             42653..43381
                     /gene="sfsA"
                     /locus_tag="KB953_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HBB6865412.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            43397..44410
                     /locus_tag="KB953_000410"
     CDS             43397..44410
                     /locus_tag="KB953_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBB6865413.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            44410..45687
                     /locus_tag="KB953_000411"
     CDS             44410..45687
                     /locus_tag="KB953_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBB6865414.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            45750..48452
                     /locus_tag="KB953_000412"
     CDS             45750..48452
                     /locus_tag="KB953_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBB6865415.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            48604..48921
                     /locus_tag="KB953_000413"
     CDS             48604..48921
                     /locus_tag="KB953_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBB6865416.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            48951..49271
                     /locus_tag="KB953_000414"
     CDS             48951..49271
                     /locus_tag="KB953_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBB6865417.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            49379..50839
                     /locus_tag="KB953_000415"
     CDS             49379..50839
                     /locus_tag="KB953_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBB6865418.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            50907..51128
                     /locus_tag="KB953_000416"
     CDS             50907..51128
                     /locus_tag="KB953_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865419.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            51159..51341
                     /locus_tag="KB953_000417"
     CDS             51159..51341
                     /locus_tag="KB953_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HBB6865420.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            51341..51754
                     /locus_tag="KB953_000418"
     CDS             51341..51754
                     /locus_tag="KB953_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HBB6865421.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            51877..53058
                     /locus_tag="KB953_000419"
     CDS             51877..53058
                     /locus_tag="KB953_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB6865422.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(53129..53293)
                     /locus_tag="KB953_000420"
     CDS             complement(53129..53293)
                     /locus_tag="KB953_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865423.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            53447..53520
                     /locus_tag="KB953_000421"
     tRNA            53447..53520
                     /locus_tag="KB953_000421"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:53481..53483,aa:Arg,seq:cct)
     gene            complement(53589..54728)
                     /locus_tag="KB953_000422"
     CDS             complement(53589..54728)
                     /locus_tag="KB953_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBB6865424.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(55027..55662)
                     /locus_tag="KB953_000423"
     CDS             complement(55027..55662)
                     /locus_tag="KB953_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865425.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(55775..56410)
                     /locus_tag="KB953_000424"
     CDS             complement(55775..56410)
                     /locus_tag="KB953_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HBB6865426.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(56444..>56779)
                     /locus_tag="KB953_000425"
     CDS             complement(56444..>56779)
                     /locus_tag="KB953_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HBB6865427.1"
                     /translation="IPFKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENT
                     VMFTKKYWNEMENLKKYIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFD
                     FKKKELLGL"
BASE COUNT        18604 a         9629 c        12063 g        16483 t
ORIGIN      
        1 tattctaatg aatggtattt tagaaaattt catgaaaaag gagttagata ggtgttcaaa
       61 aattatgttt cctcactaaa aaaagaattt tcaggttatg atctttcaag atttcagaaa
      121 gatcttttgg ctgggatcac agtagctgct gtagcattgc ctttagcctt ggcttttggg
      181 gtatcaagcg gagctgatgc ggccgcaggt ttgattacgg ctatcattgc cggtatcgtt
      241 attggcggtt tatcaggcgg tttttatcaa atatcaggtc cgacaggtgc gatggcagct
      301 atcttgatgt ctattgcggc aacaaaggga atgcaaggtg tattgcttgc tactttttta
      361 gcgggtctat ttttacttgc agcaggactt ttccgattag gaacactgac ttcttttatc
      421 ccagcacccg taattacggg gtttacttca ggtattgcta tcattattgc attaggtcag
      481 atcgataatt tatttggtgt tcactcggaa ggtacaaacg ttattgaaaa actaagtagt
      541 tatcaaacga taggatttcc gataaacaca gcctctttga tcatgggagg gctagtcatt
      601 ttagggatgt ttatctatcc aaaaaaatgg gcgaaacgaa tgcctagttc gctgctggct
      661 atcattttgg caacagcagt tatgctattg ttccatttac cagtagcaac tgtgggtaag
      721 attccacaaa cattgatcag tagtaatcgt ttgaatatgg gagattttag cttttctgcc
      781 ttgcaacaag tggcagttcc agctattagc attgctttat taggaatgat tgaaagcttg
      841 ctatgtggtg cttcggctgg ccgaatggca aataagccct tagatagcaa tcaagaatta
      901 atggcgcaag gaattggaaa tctgttgctt cctttctttg gcgggattcc tgctactgcg
      961 gccatcgccc gtacaagcgt ggccatcaaa tctggtgcac aaactagatt agctggtatg
     1021 atacatgcag ttgtcttact gctctccatg cttgtttttg cgccagtgat gtcacagttt
     1081 ccaatgccag cacttgcagg tgttttgatt gtgacggctt ggcgtatgaa tgaatgggaa
     1141 acgatcaagg agctatttac taataaatat tggtcagcag ttctattgtt tatccttacg
     1201 atgatttgta cagttatctt tgatttaagt attgctatcg tgatcggggt gatcggcggc
     1261 tgtgtatttt ttgttgtcaa aagcgctgct atcacgattt cattagaaac gatcgattgg
     1321 caaagaatgg ggctgccaga aacggaaaag ctgaataact ggacagtagt gtatattagt
     1381 ggtccactgt tttttatgtc ttcagaaaaa ctcaaaaata aattggaaga actaaatcat
     1441 cacgatggtg tcattttctc catgcgaggc gtaccaagta ttgatttgac ggcacgtgca
     1501 gtttttgatg aatttcaaga aaaagcagag caaagaggac aaacgattat ctatacttcc
     1561 ttgcagccag aagtagaaaa acaactttct cacttgtggg aacatacatt cacgcatcaa
     1621 catcaaacag ttgctcatgc gttgacagct ttacatgcga aatatactgc aattagccat
     1681 taaaacaatg aaaaagaaca gacaagactg taaactgaag attttatggt cttgtctgtt
     1741 ctttttaata ttgctgtaaa agtgaagcta gtacttgcca gtcttgatgg ataacaggta
     1801 caagggagcg attgtagaaa atagctgctg gatgaaaaag tggaagaatt cgatagtttt
     1861 tttctgacca ttcgtaggca tccttttgtt ctgttaattg taagacaggc cctgtataga
     1921 gttgaccatg atttttggta accgtatatt ctttgccaag taaacgttga agaccaatgt
     1981 ttccaagtgt cgcaattaat tttggctgga ccttcatcag ttcgtaatcc aatattggtg
     2041 catgcgcaaa tacttcggaa cgagtaggtg tccgatttgg ataaacagtt tctgactgtt
     2101 gtgtccgttt attgatccga tgagtcacgc gatacgggcg gctccttaca gcactggtga
     2161 tatacacctc ttctcgtgtt aagccagttg aagaaagtgc ctgcatcaat tcttttccag
     2221 cttgaccgct aaaaggaaca tgcgtatcaa tctctgtttt ccctggggct tctcccacga
     2281 gcatcagttt aggatgaaga ggtccttgcc cagcgataaa tccttctaca ggacggccgt
     2341 tcattcttaa agaagcttct tgtatcagtt cttttgggta ttccatttat gctcctcctt
     2401 tttcttttat tataagaata aaagaaacac aatgcgacta ttttatttgg tagaactgtg
     2461 aggatagaaa agcagaagta ttgcttgaat ttttgtttta ctttttgatt ttaaatttag
     2521 ttgactaacg gttgctgttc tggtaagcta gtactgttgt aattgtggac tccacagcta
     2581 caaccgctca gaacaagtaa taagcacagc atatgctgtc acttcggatg gcgagtctaa
     2641 gtatatcaga aggaggtgct ttatagcatg tacgcaatta ttaaaactgg tggtaaacaa
     2701 gtaaaagttg aagtgggaca agcaatctat gttgaaaaac ttgacgttga agcaggagaa
     2761 aaagttgttt ttgacgaagt tatcttagta ggcggagaat ctacaaaagt aggcgctcca
     2821 actgttgcag gtgcaactgt tgaaggtact gttgaaaaac acggcaaaca aaagaaagtc
     2881 gttactttca aatacaaacc taaaaaacat tcacaccgta aacaaggtca tcgtcaacca
     2941 tatacaaaag tggttatcga cgcgatcaac gcttaatagc gtttgagaaa gaaggcctat
     3001 atatgatcaa aggaacattt aagcgaaatg acgcaggtca gatcgtttca ttcactttga
     3061 ccgggcatgc tgatgctggt ccttatggaa gtgatatcgt ttgtgcgggt gtttctgcgt
     3121 tagcgatcag cactgtcaac gggattgcct cattagccgg ttttgagccg attgttgaaa
     3181 tgaatgaaga agaaggcggt tatctttata ccgaagtcac ttcaggaatg acacaagaac
     3241 aaaacaatat tgctgagatt ctattagaaa atcttttatt aggattacaa tcgatagaag
     3301 ctgaaaactc agaatatatc caaattaaaa caattaatga taaataggag gtgcagacca
     3361 tgttgttatc tatgaatcta caattatttg cccacaaaaa aggtggcggt tctacatcta
     3421 acggccgtga ctcagaatca aaacgtctag gtgctaaacg tgctgatgga caaacagtta
     3481 caggtggatc aattctttac cgtcaacgcg gtacaaaaat ctatccaggt gtaaacgtgg
     3541 gtatcggtgg agacgacact ttatttgcaa aagttgatgg cgtagtacgt ttcgaacgta
     3601 aaggccgtaa caaaaaacaa gtgtctgttt atccagtagc tcaagaagct taattgaatc
     3661 ttatgattaa agaggttgtg acaagacttt tgtcacagcc ttttttgttt ataaaaataa
     3721 aagttgtagt ggaggctacg aagtttactt tgtcttttta cacttaatat ttttttgcca
     3781 atttggaatc aggatgtttt gataactatc gttttcaatt gatggaagct atattgtttg
     3841 ctatcagtga aataattata tggtaaacag tgttcttttt cttgtttcca ttataaaaat
     3901 cgtatcggga tttttcgttc ttttaaattg aacatttcta tctatccgcg tgttatcaac
     3961 aaaacaaaat caggtaaata gttgacgaaa cagaaataaa tacatagtat tatgttttga
     4021 agcctaagta caatagtcta tagaaaagga gcgataccat tggcaaaacg cattgaccga
     4081 atttatatgt atgtcaaaga aaaaaccgct catttaacaa aggcagaata tgatcaagga
     4141 gtaacaacac aggaaattgc tgaagtgtta gggattcagc gaacaaatag cagtaaagat
     4201 ctgaatcagc tagtgcgcga aggaaaactg ataaagacag acggacgacc agtacgttac
     4261 atatatcaaa cactcgttac gcacgtaaag ccagttacaa aacatgtagt cagttacaaa
     4321 gaaacgccgg tctctgaaga aaaaaataca ctccaaatag atactaagga tatctttgca
     4381 aaaatcattg gagctaacgg cagcatgaaa aattcagtgg aacaggcaaa agctgcgatt
     4441 ttgtatccgc caagaggatt aaattgtttg attacaggac ctaccggatc aggaaagact
     4501 tattttgcac acgcgatgta tcattttgca aaagcgcatc atgtaataga tgaggaaacg
     4561 gaactgattg ttttcaattg tgctgattat gccaataacc ctgagctttt gatgagtcat
     4621 ttatttgggt acgcaaaagg tgcttttaca ggcgcagatg aagaaaaaat ggggattatc
     4681 gatcaagcgg atggcggtat gctgtttttg gatgagattc accggttgcc gcctgaaggt
     4741 caagaaatga tcttttactt tatggatcat ggaacttaca gtcgtcttgg ggaaacaacg
     4801 aaatcccatg aagcaaatgt acggatcgta ggtgcgacca cagaagaccc aggttcttct
     4861 ctattagaaa cttttgttcg gcgtatccca atcaatatca aacttccggc ttttgaaaaa
     4921 cgtcctgcaa atgagaaagt cgatttagta aaaatcatga ttgcccacga agcaaaccgc
     4981 acgcagcgaa agatttcttt gacagaagat gtagcaaaag cattgatcgg cagtgtgacc
     5041 tatggaaata taggacaatt gaagtcaaat atccagttag tatgtgcacg aggatttcta
     5101 aatcatatgc aatcacccga aatctcgatc acaatcgatg atttgacaga agggattcgt
     5161 agcggattga ttcaattagc aagcaaccgc agtgcaatgg cagagctttc taaaatattg
     5221 gagccaaaga taactgtagc accaaatgat acgatcatga aaatccaatc ggactcctat
     5281 gagttacctt ataatctata tgatatcatc ggagataaag cagctttact gaagtcggat
     5341 ggtttggatc aagaagcaat caatcatttc atttctacag atatcaacat ccatctgaag
     5401 tctttctata aagaccatgg tttttctttt aatgcagaca gcaaattagc agaatttgta
     5461 gatccgaaaa tcatagaagt gaccaatcag atttatgtaa tggtcaaaag atctttacca
     5521 tatgagtttc agcaaaactt tatttatgct atgagcttgc atatcagttc ttttttgaaa
     5581 aaaatcaact taggagaaga acggcatacg aatgataaca tccgtgagat ggcttttgat
     5641 tttccagatg aatatgctgt tgcgaaagaa gtgcgtagtt atatcgaaac gtattttcaa
     5701 gtggagattc cagaaagtga agactattac ttgaccgttc tactcgtctc gttacgggca
     5761 aatcaagcaa gcggcagaat cggtgtagtc gttgcagctc acggaaacag cacagcaagc
     5821 agtatggtcc aagttgtcca acagttgtta gatgcagaga acgtacgagc agtagacatg
     5881 cctttggata tggatccgaa aacagcattg gaccgaattg aacgcaatgt acaagaagta
     5941 gatgaaggaa gcggggtcat actcttagta gatatggggt cccttgcgtc gtttaattcg
     6001 caaattcaaa gagacactgg aattccagta cgtactgttg atatggtaac aacttctcta
     6061 gttttggaaa ctgtacgaaa ggtctcagta ttagggacag atctagatat gttgcatgaa
     6121 tcattgaaaa attttcgagg ttacgcagaa atatcttctg tagaatctgt tcaggaagca
     6181 aaacctattc ataagaaaaa agctgttttg gctatctgcg catctggcga aggtacagca
     6241 caaagaatca aagaattgat cgaacgagcg gtaaacaagc gtcaagaaac agaattagaa
     6301 gttctagctt tgtcgattgt ggaagtgaaa gaagaactgc cagttatcca aaaagaatat
     6361 cagatcatag ctactactgg gattactgat ttgaatatcc aagccccatt tattccttta
     6421 gaacgtttta ttgatcaaaa tatcgaagtc attttggatc agttgctgat ggaatcagaa
     6481 ttggaggaaa ctgaattcat ctccctagat gaagagtcag ctaaaaatac gtgtgttgag
     6541 tttatctctg ataacttcat ctttattaat ggtagtaagt taatcgatcc aatgtggcaa
     6601 ttttctacac aaatctctca gacgacggga atcggcgatg aagaatatgg attcaaaatc
     6661 aacctagtga tgcacacagc aggaatgatc gaacgtatta ttcggaatga accattgact
     6721 gtggaagaaa acgaattgac aaatacaacg aatgatcctc tttatagcca attggcagcc
     6781 tcagttgttc tgttggaaga tcagataaaa gtaaaagtgc cgatagaaga aatgtattat
     6841 ctgctacgtc tagtccataa tcagttagat aaaaaagaat acactgtgcc atgaatacac
     6901 aaaaacgata cacttttata gtgtatcgtt tttgtgtatt ggttaagaat cctttaaatt
     6961 caattagtgt atcgtaaaaa agttggcacg ctttttgctt atagtagtgt gtgctaggag
     7021 gtggttgttc acacagaaat gataggggtt tctgtgaagt agtgaggaag aaggagtttt
     7081 gcagatggat attcgattgg cacgtataga tgatcgttta atccacgggc aagtcgcaac
     7141 agtttggtcg aagcaaacag gtgttgaacg tatcattgtg atcagcgatg tagtcgctca
     7201 agataaattg agaaagtttc tactaaaaga agctgcaccg ccggggatca cagcaaatgt
     7261 aataaccgtg cagaaaatga ttcaaatcta tcatagtgga ttattatcac gagcaaaagt
     7321 gatgctttta ttcaccaatc cccaagatgt tgaagcggtt gtccgctgcg gtgtacagtt
     7381 gaagtcgttg aatatcggcg gtatgagttt tactgaaggc aaatcaatga ttaccaattt
     7441 tgtttcagta aatcaacaag atattgattc ctttcgttac ttgaacagtc aagaagtgga
     7501 gttagagatt cgaaaagtac ctgctgacag gaaaatcctt ttgatggaac tactagcgaa
     7561 aatcaaaaag gtgtagcaag aatggttgtt ggaaaaggtc aaataattct gttgtaatgg
     7621 ttagcaatta ttaatatttt tgaatttgtt acaacaaaag taaataggag gtataaatat
     7681 ggtagggatt atcctagcaa gtcatggaca atttgctgaa ggaatcttac aatccggttc
     7741 aatgatcttt ggcgaacaag aaaacgtcaa agccgtgatt ttgaaaccaa gtgaaggtcc
     7801 agatgacttg agagccaaat tggaagaagc ggttgcttct tttgataatc aagacgaagt
     7861 gttattctta gttgatttat ggggcggaac accgttcaat caatcaaaca cattattcga
     7921 agagcacaaa gataaatggg caatcgtcag cggtttgaat ttaccaatgt tgattgaagc
     7981 ctacgcatca cgcttttcaa tggaatctgc tcatgaaatt gcagcacaca tcattgaaac
     8041 agcaaaagac ggtgtaaaag taaaaccgga agaattagaa ccggcagaag caccgaaagc
     8101 agcagttgaa gacgcacaac ctaaaggagc acttccagaa ggaactgtgg tcggagacgg
     8161 aaaaatcaaa tatgtattag cacgtgttga ctcacgtctg ttacatggac aagttgcgac
     8221 agcttggaca aaagcagtgc aaccaaaccg tattatcgtt gtttcagatg cagtatcaaa
     8281 ggatgatctg cgtaagagac taatcgaaca agctgctcct ccaggtgtaa aagcaaatgt
     8341 tattcctatt agcaaaatga tcgaagtagc aaaagaccct cgttttggaa ataccaaagc
     8401 attgctttta tttgaaaatc ctgaagacgt attgacagct gttgaaggtg gcgtagacat
     8461 caaagaatta aatgttgggt caatggcgca ttcggtcggt aaagtcgttg taagtaaagt
     8521 attgtctatg ggacaagaag acgttgaagc gtttgagaag cttgaacaaa aaggtgtgaa
     8581 attcgatgta cgtaaagtac ctaatgattc acgcgataac atggatgata ttttgaaaaa
     8641 agcaaaagct gaattagcaa aagcgtaata aataatatta aaatttatag gaggcttatc
     8701 atgtctatta tttcaataat tttagtcgta ttagttgcct ttctagctgg tatggaaggt
     8761 attcttgatg aatttcaatt tcatcaacca ctagtggcat gtacattgat cggtttagtt
     8821 accggtaacc tcgaagctgg tatcgtactt ggcggtacgc ttcagatgat cgctttaggt
     8881 tgggcaaata tcggtgccgc tgtagcacca gatgctgcat tagcatcagt agcatcagca
     8941 attattttag ttttaggtgg acaaggtgtt aaaggtgttc cttctgcgat cgcgattgcc
     9001 gttcctttgg ctgttgcagg gttgttctta acaatgatcg tacgtacaat cgctgttcca
     9061 atcgttcatt tgatggatgc ggctgctgaa gaaggaaaca tccgtaaggt tgaaatgtgg
     9121 cacatcattg ctgtatgttt acaaggggtt cgtatcgcgg tcccagcagc agcgttgcta
     9181 tttatcccag cacaaactgt gcaatcattc cttgaatcaa tgccagcatg gttaacagat
     9241 ggtatggcta tcggtggggg aatggtcgta gctgtaggtt acgctttggt aatcaacatg
     9301 atggctacaa aagaagtatg gccattcttc gttattggtt tcgttgtagc tgcaatttct
     9361 caattaacat tgattgctct aggtgcttta ggtgttgcat tagctcttat ttacttgaac
     9421 ctttctaaaa tgggtggttc atcaaataat ggaggcggaa gcaactctgg tgacccttta
     9481 ggcgacattt tgaatgacta ttaatcttga aggaggaaaa taaaatggca gaagaaaaaa
     9541 tcaaattatc aaaaaaagat cgtttagctg ttgcgtggcg ctctaccttc attcaaggtt
     9601 catggaacta cgaacgtatg caaaacggtg gctgggcatt ttctatgatt ccagcaatca
     9661 aaaaattata caaaacaaaa gaagaacgtt ctgcagcatt aaaacgtcac ttggaattct
     9721 ttaatacaca cccatacatt gcttctccaa ttttaggggt tacattggct ctggaagaag
     9781 aacgtgcgaa tggcgcgcct gttgatgatg tagcgattca aggggttaaa gtaggtatga
     9841 tgggtccttt agctggtgtt ggtgacccag tgttctggtt tacagtacgt ccaatgctag
     9901 gtgcactagg tgcttcatta gctatgggag gaaatatcct tggaccaatc atcttcttct
     9961 taggttggaa cattattcgt tgggcattca tgtggtatac acaagaattt ggctacaaag
    10021 ctggttctaa aattactgat gacctttctg gcggattgtt acaagacgta acaaaaggtg
    10081 cttcaatttt agggatgttc gtattagctg cccttgtaca aagatgggta tcaatcaaat
    10141 tcttaccagt tgtatcaact gtaaaattag ataaaggtgc ttacatcgaa tgggataaat
    10201 tgccttcagg tggtgaagga atcaagagtg catttgaaca agttaataat ggcttagccc
    10261 tttctcctga aaaggtaaca acattacaag acaacttaga ccaattgatt cctggtttag
    10321 ctgcattagg attaacattg ttatgtatgt ggttgcttaa gaaaaaggtt tctccaatcg
    10381 ttatcatcct tggtttattc gtagtcggtg ttgttggaca cgtcatcgga cttttataat
    10441 ccataaaaat atagctcggg cgttgtcccg agcttttttt atatagtata ctgttcataa
    10501 acaaatatct ggagggagaa aatggttcaa tcaatcaata caaaagtaga cttggtaatt
    10561 gatgctactt cgtttacggg tctgacagat tacgggcaaa tcatgatagg taataatggt
    10621 tttgagtttt accatgcgcg agatagacgc aaatatatcc aaattccatg ggaagaagtg
    10681 gattatgtac tagcatcagt catgctaaag ggaaaatgga ttcctcgata tgccattcaa
    10741 acgaaaaaaa atggtacata cacattttct tccaaagaac ctaaaaaagt gcttcgtgcg
    10801 atcagagaat atgtagatga agatagaatg gtacaatcat tgagtttctt tgatgtagtg
    10861 aaacgtggta ttcgtagtat gtttaaacgc aagtaaaaaa taaagaaaac ttctaagagg
    10921 aggttttctt tattttttta ctttgcagaa gaaaatatga aatagcaaaa ccaattattt
    10981 gtccccttta aatcccatat gctaaaattg attagcaaag aaggaggtta tattatgagt
    11041 tggttatgga cattgattgt tggagccgtg atcggagcaa ttgcaggtgc gatcaccagt
    11101 aaaggaaatt cgatgggctg tgtaacgaat atcattgctg gactagtcgg ttcaagtatt
    11161 ggacaagcga ttttcggcga atggggacca aaagctgcag gtatggcact tattccatct
    11221 attttaggcg cagtgatttt ggtagcagtt gtctcatttt tctttggaaa acgatcataa
    11281 gtttttacaa aatagtaaac taaaaggagt tgcagcgaga ctgtgactcc tttttggctt
    11341 tatttattta aaataaaaag aagttttccg gaaattttta tttaactgtc gtgtacgaag
    11401 aaaagtttgc tataatggaa aaaagcgtgc aaagaaagag ggagtttcat tatgatgcta
    11461 cgtgtagaaa aattgcgcaa aaaaatgcaa gaagaaaatt tggattcatt tttggttacc
    11521 agtccgtaca atcttcgtta cttaacgaac tttacaggta cgacaggatt agctgttatt
    11581 actttggaaa aagcattttt tattactgat tttcgttata ccgagcaggc ggcagcgcag
    11641 gctcaagggt ttgaaattat taaaaatgtc ggacctattt ttgatgaggt agcaaactta
    11701 gtacgcaaag aagaactgca ctcattagct tttgaggaaa caacagtctc atttttagaa
    11761 tattcggttt tagaagaaat catcgatgcg gaattagtac ctgtttcaca gatgatcgaa
    11821 gaactcagag aagtgaaaga tgaagaagaa atcgctatta tcgaaaaagc atgtcatatt
    11881 gcggatatgg catatgatca catcctgaaa atgatccaac cgggcatgac tgagattgaa
    11941 gtcgcaaatc aactagactt ttatatgcgt tctcttggcg cttcaggtgt ttcttttgat
    12001 acgatcgttg ctagcggatt gcgttcggct atgccgcatg gtgtagcaag caaaaaaatc
    12061 atcgaacaag gtgacttgat cacattggac tttggttgct attacgaagg gtacgtttcc
    12121 gatatgacac gtacattcgc tattggtgat cctggggaaa aattaaaaga aatttatcaa
    12181 atagtcttag atgctcaatt agcagttatt gatgctgcaa aaccaggaat gactggtgtc
    12241 caattagatg cagttgctag agattatatt tcaaaacatg gttacgggga agcatttgga
    12301 cattctacag gacatgggat cggtttagag atccacgaag gaccaaacgt ttcttctcgt
    12361 gcagaaaagc aatttgtacc aggaaatgtg atcactgatg aaccagggat ctacttacca
    12421 ggaatcggtg gagtacgtat tgaggatgac ttgttgatca cagaagatgg aaatcgtgtg
    12481 ttgactcgtt caccaaaaga attgatcatc ttatagtttc atcaatagaa gaagtaagcg
    12541 taaaaatcag tgttcacaga ctttgttata gaaaatcact cgatttaatg atacactata
    12601 gaagaacttt taagctgtaa gagataataa agttgggtgt tttctccaat tcatagcagt
    12661 ttgcagctga tcggcggcag ttttcttacc acgtgatcac tatgccgcat catagagaat
    12721 tcggatgtat ctcgtgctta ggcaaaggga ggaataaata aatggcagat gaaaaaaatt
    12781 tgattttaaa cgcaaaccaa gatttaggag agatcgtgat cgctcctgaa gtcatcgaag
    12841 taattatcgg gattgcagct tctaaagtag aaggcgtata cggaatgcgc ggaacttttg
    12901 ccagcaatgt cactgaatta ttaggacgtg cagctcacgg aaaaggtgtt tacttagcaa
    12961 acgaggaaga tggcttgaaa gtagatcttt actgttatct agaatatggt gtttcagttc
    13021 ctaaagtagc aatggaaatg caggaacggg tcagccagca agtccttttc atgacggata
    13081 tcgatttatc tgaagtcaat atccatgtag tagctgttgt tcctgaaaaa ttagaacaac
    13141 caacaatcga agaacttttt gaagacgaag aggaagagaa tgaataaaga attatcaaga
    13201 cacgagattc gggaaatggc attgcaggct ttatttccat tagacttcaa tgcagaccta
    13261 acaaaagaag atgcgatttt caacgcaatc gaattagacc accgcgacat gatcaacgaa
    13321 gatgaatcag agtttgttcc tgtttatctt gatacgctag ttggcggtgt ctgtgcaaaa
    13381 aaagatgaat tggataaagt gattgaaaaa catttaaaaa acaattggca catcaatcgg
    13441 atctcaaaaa tggatttggt gatcttgcgt atcgcgattt ttgaaatgac ttatgtgaca
    13501 gatgtaccag cggctgttgc tttaaatgaa gcaatcgagt tggcaaaaac atttagtgat
    13561 gaccgttcac gtaaattcgt caacggtgtt ttatccaatg tattgaaaga attagaagct
    13621 ggggcgtaag tcctagcttt tttctatgaa tcatcataaa cctgttataa atcgggaaaa
    13681 ttctatggta gaatgaaaac acaaaaaata tctgggagtg gttagatggc agaattgatt
    13741 aacggaaaag aattagctga aaaaatgcag gcagagattg ctgaaaaaat ccaaaaattg
    13801 aaagataatg gtatccatcc cggattagtc gttttattgg taggggaaaa tccagccagc
    13861 caaatctatg taagaaataa agaacgtgta gcaaaagaaa tcggaatcca ttcattggta
    13921 gagcgttatc ctgagacgat ttctgaagac gaactacttg cagaaattga aaaatacaat
    13981 caagatccta gttttcatgg tatattggtc caattaccat tgccaaagca tatagatgaa
    14041 gaaaagatcc tgttagctat ccgccctgaa aaagacgtag acggttttca tccgatgaat
    14101 ttaggcagac tccttgcggg taaaccggac aagattcctt gtactccata cggcatcatg
    14161 aaaatgtttg aagcttattc tatcgatccg gcgggtaaac gagcagtagt tatcggacga
    14221 agcaatatcg tcggtaaacc gatggctcag ctactcttga tggcagatgc aactgttact
    14281 gttgctcatt ctaaaaccga aaatctggct gaactggcac aagaagcaga tattttagtc
    14341 gtagcaatag gacgtggaca ttttgtcaca aaagaattcg taaaaccagg tgctgtagtg
    14401 atagatgtag ggatgaatcg tgatgctaac ggtaagctga tcggtgatgt gaaatttgat
    14461 gaagtggagc ctattgcagg ctggatcact cctgtgccaa agggtgtagg gccaatgact
    14521 atcacgatgc tgatgtatca aaccgtaaaa tctgccgaag cttttggagc aggtgaatag
    14581 atggcacaag aatatttaac tgtcactaca ttaacgaagt atttgaaaag aaaatttgat
    14641 gccgatcctt atttagaacg cgtatattta acaggcgaaa tatcgaattt ccgtttacgt
    14701 gccaatgccc atcaatattt cagcttaaaa gatgaccatg caaaaatatc tgctattatg
    14761 ttcaaaggag cattccaaaa attacgattc cagccaaaag aaggaatgaa agtccttgtc
    14821 gttgggcgta tttcattata tgaagcggga ggttcttatc aaatctatgt agagcacatg
    14881 gagcctgatg gaataggtgc tttatatcaa gaattggaag aacgaaaaga aaagctgatg
    14941 aaggaaggcc tttttcaagg gcctaaaaaa atgctgccac gtttcccaaa gcgaatcgcg
    15001 gtccttacca gtccaagcgg agcggttatc cgcgatatca tcacgacggt gaaaagaaga
    15061 tatccaattg tccaattggt tttgttccca acagttgtac aaggaaacca agcagcggac
    15121 aatgttgtca ggaatatcca gagagtagaa gagatgggca atttcgatac aatgatcatc
    15181 ggtcgtgggg gtggttcgat tgaggattta tggccgttta atgaagagcg ggttgctcgg
    15241 gcgatttatc aagctaatac gccggtgatt tcatctgtag gacatgaaac agataccacg
    15301 atcgctgatt tagtagcaga tgttcgggct gccactccaa cagcagctgc agaattagct
    15361 gttcctgttt tgtcagaaga gttgatgcgc attcaagaac ggcaagctag attgcagcag
    15421 gcgtttgtac gccagatcca gcgcaaacaa gaacggttcg agcgtgctcg acgttcctat
    15481 gtatttcggc agcctgaaag attgtatgaa ggtcagacca tcaaattaga ccagttgaaa
    15541 cagcgattat atcaagctac acagcaaatt tatcatgcga aagaaaaaca aacgatcgca
    15601 ttaagccacc aattggaaca agtggctccg ctttatcgag ttaaagcagc tcgccaagaa
    15661 actgcttatc tagaaaaacg cttagaagag aaaatccaac aatatatgaa acaacagcag
    15721 cagcgttttg ctcaagctgt tcaatctctt gatttgttga gtccattgaa aatcatggga
    15781 agaggctaca gctatacgac gttaaatgat caagtagtaa aaagtgtcac cgagattcat
    15841 caaggggatt tgctgacgat ccattatcaa gatggaatcg ttaaaggaca agtacaagag
    15901 atagacaaag gagaataaaa atatggaaaa tccaactttt gaagagtcac tgcaagaatt
    15961 agaaaaaatt gtcatgcagt tagaacaagg agacgttcca ttagaatcag ctctagattc
    16021 gtttaaacga ggaatggaat taagcaaaca ttgccaagat acattagcta aagcagaaga
    16081 gaccttgacg aaaatgatga cagaatcaaa tgaagaagtt gattttgacg gaaacgagga
    16141 aagctgagat gagattaact gacttttcag ctattcatct acctgctgta gaaaaagaaa
    16201 tcttgtcttt tttagatgag catacaacgg atagaggact ctatgatgcg atgagttatt
    16261 cagtgaaagc aggcgggaaa agaatacgtc ccttattact tttaacggcc gttgcttcct
    16321 ttgatgagcc tattgatgtt cccgtctatc aagtagcagc tgctttagaa atggtgcata
    16381 cgtattcctt gatccacgat gatttgcctg cgatggacaa tgatgatctt cgtagaggaa
    16441 aacctaccaa tcataaagta ttcggcgaag cattagctat attggccgga gatggtttgc
    16501 ttacaggagc attccagcta atcagtatgg cacacttagg taattctcct aagcttctgc
    16561 tgcttcagca attagctgtt tgtgcgggat cacaaggtat ggttgctggg caagctgcag
    16621 atatcgaggg cgaatcgaaa aagctttctt tagaagaatt agcatttatt catgaaagaa
    16681 agacagggca tttgattcgt tatgccttgc tggctggtgg catattagcg aagcaacctg
    16741 aagaaatctt attgctactt caacgattag ccgaacattt aggtttagct tttcagataa
    16801 gagatgatct actcgatgtg attggtacaa cgaaaacatt agggaaaaca gctggtaaag
    16861 atgaacgtat ggaaaaaaat acttacccgc gtttattagg cctagaaaag acacgtgagg
    16921 cgttggagat tgagctgtta tccgccaaca agatcataga caaattagaa gaggacgttt
    16981 tttcttttga tggcgggctg atgcgccaga tgatcaagca atttgatgtg gagaaatgag
    17041 ggaaagaatg gaaaaagaac gcgtagatgt attagcggtc aaacaaggat tatttgaaac
    17101 aagagaacaa gccaaacgtt cagtgatggc tggtttgatt tacaacgaaa aaaacgagcg
    17161 gtttgataaa ccgggagaaa aaattccagt gagtagtgaa ttaagagtca aaggtaaaaa
    17221 acttccttat gtttctcgag ggggattaaa attagaaaaa gcactcaaag catttgatct
    17281 atctgttgaa ggaaaaacat tgttagatat tggtgcatcc actggcggat ttactgatgc
    17341 agctttgcaa aatggcgcaa aaatgagtta tgctttagac gttggctaca atcagctagc
    17401 ttggaaaata agacaagatc ctagagtcgt cgtaatggaa agagtgaatt ttcgttatgc
    17461 aaaacccgaa gactttacct taggaattcc agaagttgct gttatcgatg tttcttttat
    17521 ttcactaaaa ctgatgctgc ctccattaca tgctatttta aaagaagagg gagaagtgat
    17581 tgctttaatc aaaccccaat tcgaagcagg cagagaagct gtagggaaaa acggaattgt
    17641 tcgtgaccct cagacccatc aaaaagtttt agaagacatt ttgtcatttg cggcacaagg
    17701 tggatatgat gtgctggaat taagttattc tccgatcacg gggggagaag gaaatatcga
    17761 gttcttggct cacttgagga aagtacccga aagcggaaca atcaattcag ctattaatat
    17821 ggcagaagtg gtttcaaatg cacacgaaca gtttgaccat aaataaattg taactgtgaa
    17881 aatcaagtga gaagtatgcg gattcttctt tattatacca aggaaataag ctatcattga
    17941 ttaagaagaa tatgctaagg agcgaagacg taatgagaaa aaaagatcgg catcgtttga
    18001 ttactcgatt gttgagtgag caagatatcc gaaagcagga agaatttgtt gaaatcctga
    18061 aaagcaaagg aatatctgtt actcaagcta cgatttccag agatataaag gaactgaaac
    18121 tgatcaaggt accggcttca gatggcggct accgttatag cataccagct gaaacaaacg
    18181 aagatgtaaa tactaaattg gaaaaactat taaaagacgc tttcgtggca gttgaccaaa
    18241 tggaaaaatt tgttattttg aaaacattgc cgggaaatgc ttcggcagca gcaaatttga
    18301 ttgataaacg ttacaagaaa gaattatttt cgattatcaa tgatgacgat aatgttttga
    18361 tgattacacg aactgaagac caggcagttt tattgaagaa agacttcctg cattaccttt
    18421 aagttgagga gcgaatgtga tgctacaaga aatcagtatt actaactttg cgattattcc
    18481 ggaattgcgg ctttcttttc acgaaggaat gaccgcgctg accggagaga ctggtgcggg
    18541 taaatccatt attatcgatg ctttaggtct gttagcagga ggaagaggtt caagcgatta
    18601 tatacgtcaa ggagcagaaa aatgtgtgct tgaagggttg tttgaactac caaaacaaga
    18661 aggattctct gaactgatgg ttgaattagg gatcgaaaca gatgaggaca atctgattgt
    18721 tcgacgggat atgtccttaa ctggaaaaaa tgtttgtcga gtgaatggtc atattatcac
    18781 attagctaat ctgagaagaa tcggcagtta tttagtagat atccaaggac aaaacgaaca
    18841 tcaggaactg ctccagcctg aatcacatct tgctttattg gatcgttttg gagatgtagc
    18901 tttccaacag aagaaaaaga gttatcagca agaatatatc tcttatcgtg agctggaaaa
    18961 aagggtacga aaagtgcagc aaaatgaaaa atcctatgtt cagagaatag atatgctcca
    19021 tttccagcaa gaagaaatag ctgctgctga tttgcaagtc ggagaagaag agaaattaaa
    19081 agaagaaaga gaaaaactta gcaattatca aaaaattgct gatggattag ctgcaggtta
    19141 cggagcactg acgaataatg aacaaaacag tgtagatggt gttggcttgg ccgtttcaga
    19201 actgcaaggg atcgcccatc tagatgtgga gtatgaagca atttatgaaa atatccaaag
    19261 tgcttactat ttgctgcaag atgcaatcgg cgatatgagt cgtcagattg atttattgga
    19321 gttagatgag agtcgtttag aagaagttac gcaacgttta gaattaatcc gacagttgaa
    19381 acgtaaatac ggcgaatcca ttgagtcaat attggcttac tatgacgaaa tcaccgaaga
    19441 gctagcttct tctgattttt cagaaggtca attggacaaa atgaaagagg aactagagca
    19501 aaaagagctt ctacttcagc aacaagccgc agatcttcat gaagcgcgta aaaaaatcgc
    19561 aaaagaattg gaaaaatcca ttctgcatga attgaaaagt ctgtatatgg aaaacactga
    19621 gtttgaggta cgtttcttaa aagaagaaaa tagacaattg aatcatgatg gatttgacca
    19681 aatcgaattt tacattacga ccaatccagg agagccactg aagcctttgg tgaaagtcgc
    19741 ttctggtggc gaactgtctc ggatgttgct tgctttgaaa actatttttt cttctgagca
    19801 aggtgtgaca agtattattt ttgatgaagt ggatacaggt gtgagcggaa gagttgcaca
    19861 ggccatcgca gataaaattt ctcaaatatc gaaatactct caagttttgt gcatcactca
    19921 tctgcctcaa gttgctgcag tcgccgacta tcaatattat attgtgaaag cagtgattga
    19981 tggacgtacg caaacttctg tttcagagtt gaaaacaaaa gaaagagaag aagaaattgc
    20041 acgtatgctg gctggaagtg agattacaaa actcacactc gaacatgcaa aagaactatt
    20101 gaaactcgca aaaaggacct gagccacctc gcagtggttc aggtcctttt cagcagctga
    20161 tagctatatt ttatttttac atgacactca ttacagcagt caatacaaca gatccaatcg
    20221 ttaaaatcag cattgtccaa atgacaaatt ttgttacttt agcaaaagta cttgtttgtt
    20281 ttttttcacg cattgttttc aacctcgtca ttaattagtt gattcaagtg tacaatcttt
    20341 tttccatttc cacaagaggg cttttttata aaaacttccc tttttttaaa taataggtag
    20401 ttacgataca aataaataat gttaatagaa ctagccatat aaaggcatca tccctttcag
    20461 caataggtaa cttaatgttc attccataga tactgccgac gattgtcggt atagttaaaa
    20521 taatcgttaa agaagttaaa attttcataa tattattcag attattcgat acgattgctg
    20581 aaaaagtctg attcatttgt tctactagtt ttaattgaat gcgagaagta gtcattgcct
    20641 gctctgtttc caccaataca tcacgtaaat gatttttcaa agcatgattt tcttggaaaa
    20701 gtttattttg cgctagttct ttcaatgcat ctaaattagc agaagtcgct tctctgaaat
    20761 aaacgaggct tttttggata tccatgattt gataaagttg tttgttttca gtggatactt
    20821 gaagtttggc ttccaaagta tcgcattgag attgaagcgt agctaaataa ctgttgtaag
    20881 agagtgtgac ctgccaaatg aattgaaaga agatattgag attaggcgaa atatcacttt
    20941 ttggaaaggt ctggctaaag actttcttta aaaacagtgg ttcatgattc gtaattgtga
    21001 taagcttctt tttgggcgtc aaaataatag agagcgggta tgtatccact tgaaaataac
    21061 cgctaggact tgttttagca aaaggatatt gtaacaataa taacgctggc tttgtcaact
    21121 ttgattgatt gaattcttct gtacgggaat tttctgcatc gtccaatatg gaagtgatgt
    21181 aatctgacgg aatttcaaat tgatgtgtca gctgatgaat ctcttcttga ttaggacgtt
    21241 ctacacaata ccagatcgtt tggtcagttt cctttccggt attacgcagt ttctcattat
    21301 ctaatgtaaa atatttgatc atgattttcc tcctgttgcc tgtttctttt attataacgg
    21361 atagggaatt ggaatacatg gaatgtgttt gtaagatata gaatataact gcataaattt
    21421 aaataaaatg aaacagttgt gtaaagtata tacagtatac ttgaagcaac gataaaaaag
    21481 gagtttacgt tcatgggcac attcacacca cttatcttgt tttggtatat ttacccggta
    21541 atcgttttgt ttggttgcca atttcttgta aaattatttt cgttaaatcg tcgttttaaa
    21601 ataaaagccc ctgatttggc gattcctttt ttgtggggcg gtatgcatgc attgtctaga
    21661 aatacaggga caatttctat cttaccgttt ttctttattt ctgttttgct aatgggaatt
    21721 tgtattgctg ttttccaagc atattattat gaagaaatta tttatccgcg ttatttcaag
    21781 atgacttggc gtttggtttt tctgttgaca atcgtcttgt acgctgtctt gatcattgtg
    21841 aacatacttt cttacctatg aaaaaaatag aaaagctgta aaaagtcttt taaaaggctt
    21901 tttgcagctt gatttttaaa aaaatgttta tgaataaaaa aagttgttcc cggatttccc
    21961 agaaaaggtg gtagaaagtg ggggattgtg gtagactaat tttagttagt ggaggaatgg
    22021 ggggctttca atgttgatgg gtgaattcca acataatatt gatgcaaaag gacgtttaat
    22081 cgtgccctca aaacttcgtg aagaacttgg cgaaaagttt gttctgacac gcggattgga
    22141 tggctgtctt ttcggctatc cgatgagcga atgggaaaat ctggaagcta aactgaacga
    22201 aatgccgttg gctaaaaaag acgcacgtac ttttgtgcga ttcttctatt cggctgccac
    22261 tgaatgcgaa cttgataaac aaggacgaat caatataccc agcacgttaa gaaattatgc
    22321 agctttgaca aaaggttgcg tagtcatcgg tgtatctaac cgaattgaaa tttgggatga
    22381 gacacgatgg caagattttt ctgcagcagc agaagagaac tttgacgaaa ttgctgaaag
    22441 tatgattgat ttcggactat aagaatagga agaggctgac taatgactga aacatttcaa
    22501 cactatacag tcatgttgaa agaaacggta gatggattga acatcaaacc ggatggcgtt
    22561 tatgtagact gtacactcgg cggtgccgga catagtgaat atttattaag ccaactaagt
    22621 tctaaagggc atctgtatgc gtttgaccaa gatcaaaaag caatcgatca cgccaagctg
    22681 cgtttagctt cttatgtcga acaaggtatg gtcacattta tcaaagcgaa cttcagagaa
    22741 ttagaagaac gcttgagtga gcatgtagat aaggtagacg gcattttgta tgatctaggg
    22801 gtttcctctc ctcagctaga tgaagcagaa cgtggattta gttatcatca agatgcaccg
    22861 ttggatatgc gcatggatca gtcagctcct ttatccgctt atcacgtaat taatgaatat
    22921 tcctaccatg aattagtaaa gattttcttc cgctatggcg aggagaaatt ttccaaacaa
    22981 attgctcgtg aaatcgaacg agtacgaaaa acaaaaccaa tccaaacaac tggtgagctt
    23041 gtcgaaatca ttaaacaagt catcccagct cccgcaagaa gaaaaggcgg gcatcctgca
    23101 aaaagaattt ttcaagctgt gcggattgca gtaaatgacg aactcggtgc cgaagaggcc
    23161 tctttagaac aggcaatccg gttattgaaa gtcaatggaa gaatcagcgt aatcacattc
    23221 cattcattgg aagacagaat tgtcaaaagt atgtttaaag aatatagtac ggtacaagat
    23281 ctaccaccgg gacttcctgt catacctgac gaattccaac cagaactgaa agtcattact
    23341 cgtaaaccaa tccttccttc agaggaagag ctaacagaaa acaatcgatc acgcagtgca
    23401 aagttacgta ttgcagaaaa aataagaatg aaagaggagt agaatgatgg cagaattaaa
    23461 gaaaatgcaa gattacgaat atgatttacc tgaattggtg gatcagccgg aacaacaaat
    23521 gcctgattct tcttcgaatc aagaaatcat tcttccacaa tcaccggcaa aacgattaaa
    23581 gcagatttct gctttggaaa aaattgctgt ggcaagcgtg gcgattgcta ttgttgcttt
    23641 atgtgtttta acagttatgc tgcgcacaaa tatcagcgga gctgaaaaag aaatcacgtc
    23701 aatacaaaca gaaatcaatg ataaaaagca agaaaaaacg agtttagaac aagaaaaaaa
    23761 cgaactttct cgtactgaac ggattaaaca aatcgcagaa gaaaaaggat tgtctattaa
    23821 tgacgacaat ttaaggaaag tgaagtaaat gagtcttaaa aataaaattc ggcggtttat
    23881 ggaaaagaaa aatctaaatc cgatgaacaa tcgcaaaaaa gtaggtatca ttttatttgc
    23941 tacgagtatt ggattgttct ttttatttgc cgtccgtttc tcgtatatcg ttattggagg
    24001 tcatgttgct ggtacctcat tagctgaaaa aacaaaacag ttgtaccaag gaagtgaagt
    24061 agtaaaagca aaaagaggaa cgatttatga tcgtaacggt gtggcgctcg cagaagatgc
    24121 ctcctcttat tcaattaaag cgatactctc aaaaacatat acctctgggg ataaaaaact
    24181 gtatgttgaa gaaaagaatt ttgataaaat agcagaaatc ctgcataaga atctttctat
    24241 agacaaaaaa gatgcactga atattttaga ggacggcgca aaaaaagagc tgtatcaagt
    24301 tgaatttggt agctatggga aaaatatctc ccaagaaacc aagcaaaaca tcgaagcgga
    24361 catgaaaaaa gaaggtgtcg ctggtcttta ttttgtcgac catcaggctc ggatgtatcc
    24421 aaatggcgtc ttttcttctc attttattgg atacgcagtt ccagacaaag atgagaatgg
    24481 cttagttggt aaactaggtt tggagtctgc ttacaatgat atcttgagcg gtaaagatgg
    24541 taagattatt tatcaaaaag ataattttca aaatcctctg ccaggtacag tggcagaaga
    24601 agaaaaagct gtcgacggac aagatatcta cacgacttta gacagcagac tacaaagtta
    24661 tttagaaaca ctgatggatc aagtaaatga agaatatcag ccagaagaat tgacagcagt
    24721 cctgatgaaa gcaaaaaccg gagaaatctt ggcaatggga caacgaccta ctttcaatcc
    24781 tgaaacaatg gaaggattga ctggaaaaga tgctatctgg cgtaatttct tggtacaaga
    24841 tagttatgaa ccaggatcaa ccatgaaagt tttcactact gcagctgcta tcgaagaagg
    24901 agaattcaac gagaatgaaa ccttccaatc tggaaaaatc caagtagaag atgctacgat
    24961 caatgaccat gactttggtg aaaaaggtgt gctgaccatg cgtcaagctc tttcatggtc
    25021 aagtaacgtc gggatggtca tactggaaca gcgattaggc ggacgatggt ataactattt
    25081 acaaaaactg ggatttggac aaagtactca ttctggatta gatgatgaag taaacggtgc
    25141 tttacctaca ttaaatattg tcgaccgagc aatgagtgct tatggacaag cggtaggtgt
    25201 gacgaatttc caaatgatga aagctttcac ttctattgcg aataacggta caatgattca
    25261 acctcgatat atcagcaaag tggtggatcc ccaaactgga gaagagcgaa caactcaaac
    25321 agaagttcta gggcaacctt tttcaaaaga aacaacagaa aaagtccgtg aatatatgag
    25381 agatgttgtc gaaagcgaaa actacggtag tgcttatggt gtatatagtg tacctggata
    25441 caatgtatcg gcaaaaaacg gaacagccca aatcgcttct gatacagggg gatatcaaac
    25501 aggagataca gcctacctgt attcgattgt ggagatggtt ccttctgaag atccagacta
    25561 tgtcttgtat ctaacaatga aacaccctaa gacatatgat cgaatggcat tggctaaaat
    25621 cgcaaatcca ttgatgaaac gggcaatgga ttttaaagaa actgaagaag attcagatac
    25681 agaaacaaaa acagaaaaag tatccgtagc cgattatcgt aatcttgaag cggacgttgc
    25741 agcagctgat gctcaaaaaa gtggactgca acctgtagtg atcggtaatg gtaaaaaagt
    25801 acaaaaacaa tctactgcca acggtgatca gttgatctct ggagaaaaac tgatcctata
    25861 tactggtggt gataagctga tgcctgacgt aacaggatgg tcaaaagcag atatcatgaa
    25921 gctggggaaa atcctcggta ttgaagttag ttttgatgga gatggttatt gtgtcaaaca
    25981 agaacttgct ccatatgaaa aaatcactga agacaaactt agttttactt tagaagaata
    26041 agaagagaaa taggagagaa aagagcatgg aatggacaca agcattgatt cccattgtca
    26101 gcagttgtgc aatgacaatt gccgcaatgc cgctatttat tggttatttt caaatgaaaa
    26161 agcaaggtca ggcgattcgt gaggagggac caaagtggca caatagcaaa gccggaactc
    26221 ctacaatggg cggcttagtg tttctgatcg gctctatttt gacaggaatt tgggttggag
    26281 cttggcaaaa acagctaact cctactcttt tcatcttgct ttttgtatta gcgttatacg
    26341 gagtaatcgg ctttcttgat gattttataa aaatattcaa gaaaagaaac atgggactga
    26401 actcgaagca gaaattgatt ggacagattc tagggggaat catcttttat cttgtttacc
    26461 gttctgaagg ttatccaggt gtattgaatt tcttcggact agatctacct ttaggaatca
    26521 tttatggtat tttcgctctc ttctggcttg ttggattttc aaatgcagta aatctgacag
    26581 atggcatcga tggattagtt gctggcttag gaagtatctc ttttgcagct tacggtctaa
    26641 ttgcttggca tcagcatcag tatgatgtat taatcatttg cttaagtgtt ttaggaggtt
    26701 tactgggttt cttcgtctac aacagaaaac ctgccaagat ttttatggga gatgtcggtt
    26761 cattagcgtt gggcggactt ttagcggcaa tttctataat gctaaaccaa gaatggacat
    26821 tgcttttgat tggtttgatc tatgttatgg aaacagcaag tgtgatgctt caagttactt
    26881 cctttaagct tacaggaaaa cgaattttta aaatgtctcc aattcatcat cactttgaaa
    26941 tgtgcggatg gtcggagtgg aagattgata cagttttttg gttgatcagc attgtgactt
    27001 cgttgatcac attatggatc gtttggtagg agatgacggg aaatgaaaaa aatatcaaca
    27061 tatgaaaata agaaagtgct tgtcttagga ctcgcaaaaa gcggagtaag tgcggccaaa
    27121 ttattacatg aattgggcgc acttgtcacc gttaatgatg gaaaaccttt tgacgaaaat
    27181 ccggaagcac aagagctgct ttctttaggg ataaaagtaa taacaggtag tcatccaatc
    27241 gaattgttgg atgaagaatt ttcattgatg gtcaaaaatc cagggattcc ctacagtcat
    27301 cctttagtag caaaagccca agaaatgggt attccggtaa ttacggaagt tgagctagct
    27361 tacgaagtag cggaatgccc aattattggg attacaggaa cgaacggaaa aacaacgaca
    27421 acaacgatga caggactact actgaatgca ggagcagatc aaggaattgc acgtttagcg
    27481 ggtaatattg ggtatccagc aagtggtgta gcgcaagaag caaaatcaga agataaaatt
    27541 gtgatggaat tgtctagctt ccaactcatg ggaatcacag atttccgccc tcatatagcg
    27601 gtgattacca atatctatga agcacatatt gattatcatg gtacaagaaa agaatatgtc
    27661 aaagctaaat ggaaccttca aaaaaacatg acagaaaaag attatctgat ccttaactgg
    27721 aatcagtcag aactacaaga attagcgcaa agaacaaaag cacgcgtact gcctttctcg
    27781 acaaaagaag tgttggaaga cggtgtgtat gctgacgatt actcaatata ctataaaaaa
    27841 gaaaaaataa tggaaatatc tgaacttggc gtaccaggta agcataatgt agaaaatgca
    27901 ctagctgcta tttctgtagc aaaattgtat ggtatatcaa atgaagctat acgagaaacg
    27961 ttgcattttt tccatggcgt accacatcgt acgcaatatg taggagaaat ccaaggcaga
    28021 aaattttata atgattcaaa agcaaccaat attttagcga ctaaaatggc tctaagtgga
    28081 tttgaaacgt ctaaggtcgt gttattggct ggcggattag acagaggaaa tacatttgat
    28141 gaattaattc cttcattaaa aggaatcaaa gcaatggtcg tatttggcca aacaaaagaa
    28201 aagttgatgg atgctggaaa aaaagcaggc atcgagacaa tagtaacagc cgattctgta
    28261 gaacaagcag ttcctttagc attagaaaac agtacagatg gtgatgtagt tttattatca
    28321 ccagcgaatg ccagctggga tcaataccca aattttgaaa ctagaggaaa cagattcatg
    28381 gaagctgtga accgattaaa atagaaaaag tagaagcgag cgataaaaaa tgaaaatttt
    28441 ggttacaggc ggaggaacag ggggacatat ttatccagct cttgcctttg tcaattatgt
    28501 aaagactcaa gaaccgaatg ctgaattcat gtatgtggga gcaaaacgag gtctagaaaa
    28561 caaaatcgtc cctgatacag gaatgccttt ccataccttg gagattcaag gatttaaacg
    28621 aaaaatttcc atgcataaca tcaagacggt ccgattgttt cttaagagta tccgtcaagc
    28681 aaaaaaaatc ctcagtgatt ttcaaccaga tatcgttatc ggtactggag gatatgtttc
    28741 cggagcggta gtgtacgctg cagccaaaat gggtattcca acagttatcc atgaacaaaa
    28801 cagtgtacca ggtgttacca ataaattttt aagccgatat gtggatcgga tcgctctttc
    28861 atttaaagat gcggcttcct tttttcctga aaacaaagca gttcttgtag gtaatcctcg
    28921 agcacaagaa gtagcagata ctaaaaaatc agatattttg aaaacatttg atttggatcc
    28981 agaaaagaaa acagtcttga tttttggcgg tagtcaagga gcacttaaaa tcaatcaagc
    29041 agtatcagtt tttttaagtg aatatattcc ttcttcttac caagttttgt atgcttcagg
    29101 agatagatat tatgaagaga tcctaaataa aataaatcct gaaatacaaa atattagtgt
    29161 ccagccttat atcaaaaata tggcagaggt catggcttcc agtgatctgc tggtggggcg
    29221 tgctggtgca acttcgatcg cagaattcac agctctgggg ttaccggcag tgttggttcc
    29281 cagcccatat gttacgaatg atcatcagac taaaaatgca atgagtcttg ttcacgcagg
    29341 agcagcaaaa atgattgcag ataatgaact aacaggtgaa tcactaagtc agacagtcaa
    29401 cgaaatcatg ggtgatgaag aattgcaaaa gcaaatgtgc cgagcttcta aagaacaagg
    29461 aatccctgat gcttcaaaac gtctttatga cttagtaaaa caaataatca aaaaataaag
    29521 gcggtggtct tagtgagtaa gaaaaaagaa ccaaaaaaat tgtctcatcc gtcttctgat
    29581 gaaaacaatt tgactccttg gcaaaaggct aaccgaaaat atctggcaga acaagagatg
    29641 aaaaaccaag agccaaaaaa agaagacata acattagata cacccctttc agattctgaa
    29701 gaagaaaaaa cggttcaaga ggaacagcaa gaagaaagcc agtccgagaa aaaaatcgaa
    29761 ttttttgaag agatcaagca aaagaaaaac ggtggaccat ataacggttc ttttttgaac
    29821 cggctgccca atttaaaaag tcaacggcac aaagtactat ttcgtcggtt agctttgatc
    29881 atcactgtat tgggtatacc gctgattttt ttgatctatt atgtttctcc ttatagcaaa
    29941 ttacaagcag ttgcagtatc aggcaataag acagtcaaca gccaagaaat catctctgat
    30001 acaaaattgt cattaggaga aaatgtttgg ggacagtatt ttcatcgatc tacttatatc
    30061 gatcgcctta aaaaagcaca gccaagaatc gaaacagcaa atatacattt caaagggatg
    30121 aatgaattcg atttggatgt aacagaatat aaagagattg ctttgatcgc aaaaaacaat
    30181 caatactacc cagtgattga aaatggaaca gtattagatg aaaaagtagc aaatcctaca
    30241 aaaaatctcc ctatcttgga agagttcaaa gactctgcaa aaatcaaaga attaacaaag
    30301 caatacaatc agttatcatc agaattacaa aaagcaattt ctgaaatcaa gtacacgcca
    30361 agagcaagca ataaaaatct gatccaattg aatatgaatg acggcaatca agttattgta
    30421 aatatcaata atcttgcgaa ccaaatgaaa tattattcgc aagtagccaa agatatggat
    30481 gaaaaaggtg tgatcgacat ggaagttggt atcttctcgc atccttacgg tagcactcaa
    30541 gaaacggaag aatcgggcca atcggaagaa acaactgaaa gtgaagaaaa tgcggatcaa
    30601 acaaacgaat caagtgaaga ggcagaaacg caaaaagcac aaacacctgg atcgtctagc
    30661 acaaacggat catctgattc aaatgattca agtgaaacaa gtcaaataga agataatcca
    30721 agtacaactc cgtcaagttt aaatgaaaat tgataaaaca actttaaaaa gtaagaatgt
    30781 actttctaaa aaccagtaat ttatggtaga attggaagca gtgaatatat ctattaataa
    30841 gtatatttta aaacggacgc tagttgttcg cttgtatatg gaatagtaag taggagggaa
    30901 tcccattcat ggcaaaaaca ggaatgtatg taggccttga tattggaacg acatctgtta
    30961 aagttgtcgt ggctgaatat atcgacagcc aaatgaatat tattggagta ggaaacgcaa
    31021 aatcagaagg gattaaccga ggcattatcg ttgatatcga taaaacggtc caagcgatac
    31081 aacgagcagt acgacaagca gaagaaaaag caggtatcca gatcaaaggc gtaagtgtcg
    31141 gtttgccagc aaacatgctt gaagtagaaa attgccaggg aatgattgca gttaacggag
    31201 attcaaagga aatcaccgat gaagatgtca gaaatgtggc ttcagctgcc cttgttcgtt
    31261 caatacctcc tgaacgtcaa atcgtgtcaa tattgccaca agattttact gtcgatgggt
    31321 tcgagggaat caaagatcct cgtggaatga tcggcgtaag attagaaatg tacggtctgt
    31381 tgtttacagg acctaaaacg atcatccata acatacgtaa atgcgtggaa aatgccggat
    31441 taatcgtcaa cgaaatggtg attactcctt tagcgctgac tgaatcaatt ctttctgacg
    31501 gagaaaaaga ttttggtacg atcgtcattg atatgggtgg cggccaaaca acaacggctg
    31561 ttatgcatga taagcaattg aagtttacca atcttgacca agaaggtggc gaatttgtca
    31621 ctaaagatat ctctattgta ttaaatacgt catttaacaa tgcagaagcg ttgaaaataa
    31681 actacgggga tgcataccca gaacggacat caccagatga agaatttcca gtagatgtga
    31741 ttggtcagtc agaaccagtg aaagtcgatg aacgctactt gtcagaaatt atttcagctc
    31801 gaatggaaca gattttcaac aaagcaaaag atgcattgga ccaaatcgaa gcattagagt
    31861 tgccaggtgg cgtcgtattg acaggcggag ctgctagtct tcctggtgtt gtggacttgg
    31921 cacaagaaat cttcggcgta aatgtaaaat tgtatgttcc aaatcacatg gggctgagaa
    31981 acccggtatt tacaaatgta ataagtatcg tggattattc agcaaacttg agtgaagtgt
    32041 atcaattggc aaaaattgct gttacgggtg aaacctcagc agctcgtcaa atggtagttg
    32101 aacaagaaac aacaaacaca tatgagagct atgaagcacc tgaagaaaca atctacgatg
    32161 agccggaacc aaaagagtcc ggagaaaatg taaaaaataa aatcaagggt ttctttacaa
    32221 atatttttga ttaattcatc ctgggaggaa taagatatgg aattctcaat tgataataat
    32281 atcaatgacg gcgcagtaat caaagtcatc ggagtcggtg gtggcggtgg aaatgctgta
    32341 aaccgaatga ttgaagaaaa cgttaaaggt gttgaattca ttacagccaa cacagacgtg
    32401 caagcattaa aaaattcaaa agctgaaaca gttatccaat taggccccaa atatactcgt
    32461 ggtttaggtg ctggttctca gccagaagtt ggacaaaaag cggcagaaga aagtgaacaa
    32521 tccttacgcg aagctttaga cggtgcagat atgattttca tcactgcagg aatgggtgga
    32581 ggaaccggta caggtgctgc accaatcgtt gcaggtatcg cgaaagaatt gggcgcattg
    32641 actgttggtg tcgtgacacg tccattcact tttgaaggac cgaaacgtgg ccgttttgct
    32701 gcagaaggta tcgctcgttt gaaagaaaat gtggatacat tattaattat ctcaaataac
    32761 cgtttattag aagttgtaga taaaaagaca ccgatgcttg aagctttccg tgaagcagat
    32821 aatgttttac gacaaggtgt acaaggtatc tctgatttga tcacagcgcc aggttacgtt
    32881 aaccttgact ttgctgacgt gaaaacagtg atggaaaacc aaggtacagc attgatgggg
    32941 atcggtgtgg ctagtggaga agatcgcgta gtagaagcaa cgaaaaaagc aatctcctct
    33001 ccattgcttg aaacatctat tgatggagct gaacaagtac ttcttaacat cacaggcggt
    33061 ttggatatga cattgttcga agcacaagat gcttcagata tcgtagcaaa tgcagcaact
    33121 ggcgatgtaa atatcattct aggtacatct atcaatgaag aaatgggcga tgaaatccgt
    33181 gtgacggtca tcgcaacagg tatcgatgaa tcaaaaaagg aacgcaagtc tagccgacca
    33241 gctagacaag cacaaatgca atcacctgct caaaagactg tattagatat ggatcaagct
    33301 aaaccaactt cttcagaaga agaaaacagt tttggcgatt gggatatcag acgtgaacaa
    33361 aatgttcgtc ctagagttga tgattccaat tttgacaata tcgaaaaaaa agaatttgat
    33421 acattcaacc gcgaagaaac aaaatctcat ggcgatgatg aattaaatac gccaccgttt
    33481 ttccgtcgta aaaaataaag gagaggaaga gcacatgatt gctgacaact tgcagaaaat
    33541 caagcaagaa attcaggatt cgtgtgctct tgttcatcgt gatccgaaag aagttacatt
    33601 agtagctgta acaaaatctg ttgaatccgc tcaagctgaa gaattggtta gagctggcgt
    33661 tatgaattgt gcagaaaacc gagtagataa attattagaa aagaaacaag atttatttga
    33721 ttttcctgaa ataaagtggc atttaattgg taatttacag cgcagaaagg taaaattgat
    33781 tgtaaatgaa attgattact ttcatgcgtt ggacagttta agattagcag aagaaatcca
    33841 gaagcggtta gagaaagaat tatcctgttt tattgaagta aatgtttctg gtgaggaatc
    33901 aaaacatgga atttcaccag atgagctttt accgtttgtg gaatctttag ctgcctgcac
    33961 aaaaatcaag gttgttgggt tgatgactat ggcgcctaaa gaagcttctg atgagcaaat
    34021 ccataaagtg tttggtacgc tgaaagaatt gcgtgatctt gttcaagcaa aaggttatgc
    34081 ccatgcacca tgtagtgaat taagtatggg gatgagtcaa gactttcaaa ttgcgattga
    34141 agaaggtgca acatttatcc gtgtaggaac agcgctattc aaagacgaat aaaggaggaa
    34201 caaaaatgcc gcttttaaac agaggagcaa ttgctaattt tttcggatta gctgatgagg
    34261 aagagtacga ataccaagat tatcctgaaa caacaaaaaa ggctgtacag ccaactaaaa
    34321 aaaatacaac acagaatcaa tcaactcatt ctgtatctgc tgcgaatact catgaaaaac
    34381 aaaaaacggc gacttcacgt cctgtaacgc atgaaagtat tcagccggct gaaaagaaaa
    34441 aagtaattga actacatcaa tcacctacaa aaactgtagc gaaagaaaga acagcttcaa
    34501 gaacctctac acaacctaaa gcaaatcttg ctggaaaaat cactgtaatg gaaccaagag
    34561 cttattctga agctatggcg attgctaaac gaatcattgc tggagaagca accttagtga
    34621 attttcattt agtagaagaa aaacaagcga gaagaatcgt tgattttcta acaggaacag
    34681 tttatgcact agatggagat atcaaacgtg ttggagatga gatttttctt tgtacaccga
    34741 ctgggatgga gattgacagt tcaaccgctc aatcttttgc agatgcgaat ttgtttgatc
    34801 tgtaagagga ggaactacga tttatactat tgcactatta cttcatttac ttcaacgggc
    34861 agcctatttc tatagcatta tcttgatggt atatgcattg ctctcttggt ttccaggcgg
    34921 gtatcaatca gggctaggac gattattagc taagatctgc gaaccgtata tcagtttgtt
    34981 cgatcgtctg cctttgagga ttggaccctt ggatttcaca attttagggg caatcctgat
    35041 cttgaactta gccgtacaag gtttagcaca gatcgttata tggataactg tccatttagt
    35101 ttattgattg aagcaacaaa atctttattc tcagaataga taggagggga aaattttgga
    35161 tgcaaatgta taccagcact ttcgggcaga tgaacgtaca tttatcgatt cggtaggcga
    35221 ttggattgaa caggttcaag gacaatatgc accctatctg accgaatttc ttgacccaag
    35281 acaatcctat attttagaaa cactgattcg acaaagcagt gatttgcgtt tcatgtttta
    35341 tggcggatat gaacaggcag aaagaaaaag atgtttgatt ttccccgatt attacgaacc
    35401 tgtagaggaa gattttgaaa cagcattgta tgaaatccat tatcctagca aatttgccag
    35461 cctaagtcat gggaaaatcc ttggaacttt agtgggaacg gggatcaaac gctcttactt
    35521 tggcgatatc atctcggacg gtgatcgctg gcaagtattt attgcaaatg aggtggatca
    35581 ttttgttgct gcacaagtaa caaagattgg aaaggttgct gtacgcttgg aaccaatcaa
    35641 atacacagaa atgattatgc cgaaagatag ctggacacaa gaaagaggaa cagttagttc
    35701 catgcgacta gacagtgtga tctctgagat ttataatatt tccagacagc ggtccaagca
    35761 gctgatcgaa gctggtaaag taaaagtgaa ctggacagaa aatacaagac ctgattttgt
    35821 acttgaacta ttagatatta tttcagtcag aggttatggc agagtccaga tccaagaact
    35881 tgaggggaag acgaaaaaag aaaagtacag ggtactgttc ggtgtattac gtaaataaaa
    35941 taaaaaggtg gtttactcat ggctttaact ccattagata tccaaaataa gacattcccg
    36001 acaaaaatgc ggggatataa tcaagacgaa gtagatgatt tcttagattt agttgttcgt
    36061 gactatgagg aattgactca aagaaatcga gaattagaaa aagcagtcaa acattctgaa
    36121 gaaaaacttg agtacttcaa tgaattaaaa gatgctttga accaatcaat catcgtagct
    36181 caagatacgg ctgataaagt aaaaacaagt gcaagcaagg aatccgaagt gatcgttact
    36241 tctgcacaaa acaaagcaga tgagttagtt gcgaacgctg aaaaacgtgc acatcaatta
    36301 acaactgatg cagaagaaaa agcaagaaaa atcttgacag atgcaacaga aaaagcaaga
    36361 cagcttgcga cagaaactga agatcttaag aaaaaaacac gagtattcca tcaacgtatc
    36421 agcttgatgc ttgagtctca attagaacaa gtgaaaagtc cggaatggga tgaaatttta
    36481 caaccattct ccagctacgt gactgacagc catgaagtga tcaaagaagt tttagcaaaa
    36541 cagcttgaca atgaaaatga tactgatgta aactcagaca caggagtcat agaagcacct
    36601 atgacggcat ttgatacaca ggctattgat atctcgatcg tgcctggtga aaatgaaaac
    36661 gagtagaaca gaaaagcact ttgttattct taaagcgagc cgatgagagt ggaagttcgg
    36721 taagacactg catggtgaaa gatcctctac ttgagacctg cttttccgaa attagagtaa
    36781 gaagcagcgg ctgacctcgt tattggtcac agagagtttg cactagatag atgcaactaa
    36841 atttgggtgg taccacggca tgttcgtccc ttgcggctga acatgctttt ttgtgtccgg
    36901 aaaacaaata aaggagtgta tggttgaatg aaaatgaaag aaaccttgca attaggaaaa
    36961 acaaaattcc ctatgcgtgg taatttgcca aaccgagaag gcgagtggca aaaagaatgg
    37021 gaagaaaatc gaatctacca aaaaagacaa gagttgaacg aaggaaaacc tagctttgtt
    37081 cttcatgatg gacctccata tgccaatggt aatatccatt taggacattc attgaataaa
    37141 atcagtaaag acattatcat tcgttcaaaa tcaatgtctg gctatcgtgc accatatgtt
    37201 ccaggatggg atacacatgg attgccgatt gaacaagtat tggctaataa aggcgtgaaa
    37261 cgtaaagaaa tgtccatggc agaatacttg gaaaaatgtc gtgaatatgc actttcccaa
    37321 gtagataagc aacgagcaga tttcaaacgc ctaggagttg caggagattg ggaacatcct
    37381 tatgttacat tagatccttc ttatgaagct gcacaagtac gcgtatttgg taaaatggct
    37441 gaaaaaggct atatttacaa aggattgaaa ccaatttact ggtctccgtc aagtgagtct
    37501 tctttagcgg aagctgaaat tgaatataaa gatgtaaaat ctccatctat ttttgtagct
    37561 ttcaatgtca aagacggcaa agatattctt gatgaagata cagcttttgt catttggacg
    37621 acaacaccat ggacattgcc agcaaaccaa ggtattgctg taaatccaaa atttacgtat
    37681 gtattggtgg aagccgatgg ccgtaagttt gtcgtagcga aagatttgct agaaacagtc
    37741 caatcagaaa tcggttggga aaatgtaaca gttctaaaag aatttgctgg acaagaaatg
    37801 gaatatatga ctgcaagtca tccattctat gaacgtgaat cacttgtgat tttaggcgat
    37861 catgttactt tagacgctgg tactggatta gtccatactg ctccaggaca tggggaagat
    37921 gactatatcg ctggtagcaa atataaacta tctgtagtgt cacctgtaga ctcaaaagga
    37981 ttgtttacag aagaagcacc tggatttgaa ggtatctttt atgacaaagc aaatcctatg
    38041 atcactgatc tattaaaaga aaaaggagct ttgctgaagc tagatttctt tactcatagt
    38101 tatcctcacg attggcggac gaaaaagcct gtgatctatc gggcaactcc acaatggttt
    38161 gcatctatcg ataaattccg tcagaatatt ttggatgaag tagaaaaagt agagtgggtt
    38221 atcccatggg gtaaaacacg tttgtataat atgatccgtg accgcggcga ttgggtaatt
    38281 tcccgtcagc gtgcatgggg agtaccactt ccgattttct atgctgaaaa tggagaagca
    38341 atcatcacac cagaaacaat cgaacatgtg gcaaaattat ttgctgaaca tggatcaatt
    38401 atctggtttg aaaaagaagc gaaagaactt cttcctgaag gatttaccca cccaggttct
    38461 cctaatggcg aatttacaaa agaaaaagat atcatggatg tctggtttga ttctggttct
    38521 tctcatgaag ctgttttacg tcagcgacca gaattatcat tcccagcaga tatgtattta
    38581 gaaggatcag accaatatcg tggctggttc aactcaagta tcacgactag tgtggctatc
    38641 aatggtgtag caccatacaa atcagttatt tctcaaggat ttgctttaga tggtgaaggc
    38701 cgtaagatga gtaaatcttt aggaaacatc attgcaccag aaaaagtgat caaacaattt
    38761 ggtgcagata tccttcgctt atgggtatct agtgtagatt atgaagcaga tgttcgtgtt
    38821 tctatggata tcttaagtca agtggcagaa gtatatcgta aaatcagaaa tacgatgcgt
    38881 ttcttattag cgaatacaga agatttcgat cctaaaaaag atactgtttc gtacaatgat
    38941 ttacgttctg tagacaaata tatgaccgtt cgattgaacc aagtaatcaa agaaatccga
    39001 gaagaagggt acgacaaata taacttcatg catatttacc gtacagttat gaacttcttg
    39061 acggttgatc tatcgtcatt ctacctggat tttgctaaag atgtagttta tattgaagca
    39121 gaagatgatt atcaacgtcg ttgcatgcaa actgttttct atcaaacagc tgtagctttg
    39181 acaaaattat tgactcctat cattccgcat acagcagaag aaatctggtc attcctgaaa
    39241 gaagaagagg agtacgttca gttaagcgag tttcctggtt atgaagagtt tgcaaaccaa
    39301 gaagaattga tggatacctg gactgctttc atggacttcc gtgataatgt cttaaaagca
    39361 ttggaagaag caagaaacag caaattgatt ggtaaatctt tggaagcaaa actaactgtt
    39421 tatccaaaac aacaagttcg tgaaatgtta aaagctttgg atgcagatat tgcacaatta
    39481 ttgatcgttt caccagacta ctttgaagtt atggcagcag atgaacaagt gccagataac
    39541 gctatggtat ttgatgatgt tgctattaca gtggaaaaag cagacgggga aacttgcgat
    39601 cgctgtcgcc aagttcgtaa agatgtagga gtagatgaaa aacttcctca tctatgcggt
    39661 cgttgtgcaa aaatcgttga agacttctat cctgaagctg tagctgaagg attcgaagaa
    39721 aagtaaaagg gaaaaggggg tgtgacatgt tctttgtcac accccctttt gttatcaatc
    39781 gtttaatttt ccacgttcac gataccagtt atctggttgc cagatccatt cgaatccgtc
    39841 tttttcaagt agttcgaaag ctgctttagg tcccattgat ccagctttgt agttgggaaa
    39901 atccggttgt gtttggtccc aagcttttcg aatgacatcc acgatatgcc aagattgagc
    39961 gacttcttcc caatgagaga agttcgttcc atcaccattc aacgcatcta atagtaattt
    40021 ttcgtaagct tctgggctat tttcaaccat ttctgcacta tttctaaatt ccaatttgat
    40081 tggttccgtt tcaaatcctt gccctacttc tttcccattc aaggataaag aaaatccttc
    40141 agttggctga atatagatcg ttagaacatt aggtggaagt gtcgtatcgt cacatggttc
    40201 atcgacagat gttttgaata cattaacagg aacttgtttg aatacgatgt tgatccgagt
    40261 ccctttttca gtcaaacgtt tgcctgtacg tacatagaaa ggaacacctg accaacggaa
    40321 gttatcgata gtgaatttgc ctgcgacgaa tgtttcagtc tctgattgag catccacttt
    40381 gtcttcttcg cgatagccct tgaattgtac accatccaat tcaccttgag catattgccc
    40441 gcgaacgaag ttttctaaca cttcttcttc gttataaatg cggatagctt tcaatgcttt
    40501 gatcttttct gtacggattt ctttttcgga aaatgctact ggcggttcca ttgcaagtaa
    40561 tgctacaacc tgtaaaatat gattttgcac catgtcttta agtgcgccac tatgatcgta
    40621 gtagcccccg cgatcttcta caccgaggtt ttcgccaaaa gtaatttgga tattgtcgat
    40681 atagcgattg ttccattgag cctcaaaaat attattggca aaacggatag ctgagatgtt
    40741 ttggatcatt tcttttccta aataatgatc aatcctgaaa atatcttgtt ctgggaatac
    40801 ttctctgatt tgctggttca attcaaaagc agactggtaa tcagaaccga acggtttttc
    40861 tatgatcaaa cgattgaagc cattttttgt cataatattt tgtgatttca aatgagaaac
    40921 gatcgttcca aaaaattgag gtgccatagc caaataatag acacggttac cgtttaattg
    40981 atagtgtcca tctaatcggt cagacaattc ttttaatgtc tgataatgcg ttgaatcatt
    41041 tacatcatgg gattgatagt aaaaatggct tgcaaaggta gttgcttctt catctgaagg
    41101 attcaatgca cggattgttt ctttgacaac ttcacggtag tgctcatcgc tccagggacg
    41161 gcgagcggtt ccgattacag caaaatgctc gcctaaattt ccttttctgt aaaggcggaa
    41221 caaagacggg tacaatttac gctgggctag gtcacctgta ccgccgaata tcgtaaacaa
    41281 tacatttttt tcattagaca tgtggataca ctcctttata ttttatcgac aaaaatcgtg
    41341 ctggccgcct taaagctgac ggggattttc tttctatcat tttggatagt gatcggtcct
    41401 tcataagctg cgatgtcaac gatctcatat tcttcgttga tattcaggtc aatagtgact
    41461 aagtaatcca acaattcttt ttcatcaagc actcgtgcga tacgcacttt tgtaccgaca
    41521 ggataggact ccaatgtttg tcggcgttct tcatggacaa cttcctcttt tttaggaatc
    41581 gatcctccat gaggacaatg ttcaggatga ttcaaatagt tttctaaatg atctgcaaga
    41641 tgttccgaag tcacatgttc caatacttca gcatcatcat ggacttcatt ccagctatag
    41701 tctaaatgtt caactaaaaa aacttcccat aagcgatgtt tgcgaatcag actgctagct
    41761 ttttgcagtc cgcttgcagt caattgtaca ccttgataag gggaatgttc tacaagctct
    41821 tctttcacaa gtttagaaat catttcactg acagatgcag ctgagacatc tagactagat
    41881 acgatttgtt tattgttgac tttcgttgta tctccaccta gttctaaaat gattttcaaa
    41941 taatcttctc gatttggtgt catattcgat catcctttca tggtaaaaaa ccatcattag
    42001 taagtgtaac aaaaaaaacg ttaattgact atttttctaa ataaaaaaca aaggattttt
    42061 agacgttttt acaacaaaaa acgaaagatg atagagaaga ctcattcttt cgtttataaa
    42121 ggatatatat atacattggg agggatctat ttttcagata acaacttata tgaagtgtct
    42181 gcagattcgt cataaagagt ttgttcggga taagaaaaga taagataaaa accatactga
    42241 tcacgtacga ctaaacgata ttcatctatt tctaccacag atgtgccaag agactgaccg
    42301 ttgatcaaaa acgtgtaatc agatgtaatc tgtaattgtt gtttttgctg gctatcacta
    42361 aagaaccatg taccttgata ctttgagagt ccttctgact tttccgcttg ttttttcttt
    42421 ttcaagtaaa taaaacttga gagaacggta atgagtgaac caagaaaata gatcattttt
    42481 tttatcacca aatcactcct ttatgagaaa ataatcgcat agacatattc tttcttcttt
    42541 atttcattgt accgaataaa agctaaaatt tctctaagac tttcgactgg tttttttact
    42601 gtttttctac aaattatgat aggatacata gtaggaagaa aggtgacata atttgaagta
    42661 tcagcaagtg gaaatagctt tttttattga ccgcccgaat cggtttatag cacattgtat
    42721 gaaaaaagac ggagaaataa ttattgcgca tgtcaaaaat acaggtagag gaaaagaggt
    42781 cctgatacca ggcgctgaag tggctattgt atttgctcct ggacctaaac ggaaaaccgc
    42841 ctatgatctg attgctgtaa aaaagaaggg tgactggttt aatatcgata gtcaattgcc
    42901 taatcggctg gcaattgatg gtattttaga cggcacgatc cttttaccag gattaactgg
    42961 tccatttacg gtatacaagc gtgaagtaac ttatcttcac tccaaatttg atatttattt
    43021 agagacggtg gtcggacaaa aagcatttgt tgaagtcaaa ggtatgacac ttgaaaataa
    43081 agcgattggt gcttttcctg atgcgcccac actcagagga ttgaaacata taggagaatt
    43141 aacgtatgcc gcacaagatg gttatgccgc ttatgtcttg tttattgctc agttcgaaca
    43201 tcttcatctg gcaacgatcc atgaagaaat gcaaccagca ctagctgata tggttcgaca
    43261 tgcgcagcaa agcggagttc aaatcctcgc ctataattgt caagtatcgg cagatgaggt
    43321 aactgtcaag caggccattc catttgatgt agatatccct tttgaagatc ctatacaata
    43381 gaaagaaggt aaaataatga tcaacttagg tattatcgga acaaattgga ttactcatca
    43441 atttgtccaa gcagctttaa gcacaaaaaa atatgaattg acagcggtat attctagaca
    43501 tttggaaaaa gcacaggaat ttggttctca ttatgaaggg gatatcgaat tcgcaacaga
    43561 tttgaagact tttttcaata ttactcatat ggacaccatt tatattgctt ctccgaatag
    43621 tcttcacttt agtcaagcta agcaagcaat tttggcagga aaaaatgtga tagttgaaaa
    43681 gccagctttt tcgacgccaa aagaaatgga tgaaatcatc catttggcaa atgaacacaa
    43741 tgtgtatttt tttgaagcag caagaaatat ccatgaatca ggttttaaaa agattgccga
    43801 ttacttacca ttaaaagacg agataatcgg tgcaaatttc agctatatga aatattcttc
    43861 gcgctatgac caagtattag atggagaaga accgaatatc ttctcgactc atttctcagg
    43921 tggtgcattg atggatttag gtgtttacct tgtatatgca gctgttgctt ggtttggaat
    43981 gccaaaagaa gtccattatt tcccaagaaa gatttctact ggtgtagatg gtcttggatg
    44041 ggggatctta cgttatgaga attttgatgt atcgatccag ccaggtaaaa tcggtgattc
    44101 ttatttaccg tcagagattt attttgactc aggaacgcta gtgatgaatg gagtcaatgc
    44161 aattgaacaa gcacaatttc atgatcgcgc gcataaagaa atcattgatt tggcggtaac
    44221 agctgcagaa aatccaatgg cagatgaagc agaggatttt gccaatgtca tggctcatcc
    44281 gaaagatcca gcttggggat tgttgtatga agagtggatc gagctagcta gaaatgtgaa
    44341 ccaagtgatt tatgatttaa gaaaaaatgg cggcatcaaa ttcgatgcag ataatgagga
    44401 agatttttaa tgaacgaatt tgtacaagta tttcccgaaa attggcagaa aaaatatgag
    44461 gataaagggt ttatctcacc ttctcttatt caacgagcag tttttcagcc actaagtaat
    44521 aaacaaagta tagtagcggt ttctcctaca ggttcaggta aaacactggc ttatctttgg
    44581 cctctactac taaacgtgga accaggtgaa gcaagtgcgt tggtcatttt tgcttcttct
    44641 caagaactag ctattcaagt agcagatgtt gcacgtgaat ggggaaaaga caaagagttg
    44701 aaagtccaat ctttggtggg cggagccaac gtcaaacgtc agattgaggg tttgaaggaa
    44761 aaaccagaaa tcttgattgg tacaccagga agaatattgg aattgatgaa agcaaaaaaa
    44821 ataaaagcac atcaagtgaa aacaatggta tttgatgaag cagaccagct atttgacgct
    44881 gggaacagcc aaatcattga tcaaattctt catcaagctc caacagagta tcagctagct
    44941 tttttttcag cgacagcaga tcgttccctt gaaagcattg aaaaaatcac tgggaaaacg
    45001 tttgagacaa ttgacgtgac agcagaggac gattctcgaa aaggactgag acattacttt
    45061 ttacgtgtac cgatacgaaa gaaagatgac tacttgagac gtctcacgca tatagaagat
    45121 tttcatggtc tgattttctt taatcaactg actgaattag gcgtaatgga agaaaaattg
    45181 ctgtatagag gtgtaccggt aggaagtctg gcctctgatc agaacaagct actccgaaaa
    45241 gcagcgctag aacaattcaa aaagaaaatg gtcagagcac ttttgaccac agatgtggcg
    45301 gctagaggtt tagatattac aggtcttcct tatgtagtga atgcagaagt tccgttaagt
    45361 gaagaagctt atttgcatcg ttcgggaaga actggtcgaa tgggtaatga aggaatagta
    45421 attacactcg tacaagataa taatctaaga gatttaaaac ggcttttacg tccatcaaat
    45481 ataacacttg aagagatttt tttatacgga ggtaagctgc aaactgagca gccaaccaaa
    45541 gaagatgcag ctcctgcagt aaataaaaag tatgcgaaaa ctttttctgg aaaggagcag
    45601 aacccagaat ataacaaaga aaaaataaga aaaaaagtta aaaataaatc aaaaaaagag
    45661 agaaataaag gagcaaggcg caaataagcg cctttttatg ctatgattat gaaaacggga
    45721 acaaaattgg ttgccagagg aggggttatg tgaaagaacg tcaagaagag atcttgcagc
    45781 tattgacaga aaaccaacaa ggattgacag caagtgatgt agctgagcgc ttaactatcg
    45841 atcgaagcaa tgcaagccga tatttaagtg aactatacaa ggcccatcat attgtaaaaa
    45901 ccgctggccg accagtcgtt tactctttgc caacggaaaa gtcaaaatct gatgaagtgc
    45961 atgtcgactc ttctacgcaa gtgacttttg aaacactcgt tggagaaaat gattctttaa
    46021 aagtcagcat acagcaggca aaagctgcga ttctttatcc accgcggggg ttgcatacga
    46081 ttatttttgg agaaactggt acagggaagt cgatgtttgc tgaatgtatg tatcattttg
    46141 caatcgattc agagatgctt tctgcagatg cgccatttgt ttctttcaat tgtgcggatt
    46201 atgctcaaaa tccgcaactg ctctttggac acatctttgg gatcaaaaaa ggggcttaca
    46261 caggagcagc tcaagatagt cccggtctaa tagccaaagc agacggcggt atattgtttt
    46321 tagatgagat ccaccgttta ccgccggaag ggcaggaaat gctgtttact ttcattgata
    46381 aaggtattta ccgtccgctt ggagaaagtg cacaagtaca tgaagcatct gttcagatca
    46441 ttggggcgac gactgaatct tcagaaagtt tcttatctac gttcaatcga cggattccta
    46501 tggcaatcac cttgccgaat cttgcttcac gttcactgga tgagcgttat gaaatcatct
    46561 ctctttttat caaacaagaa gcgaatcgtt tgaatcaacg tatcgatatc gaaaaagaag
    46621 cgattcttgc cttcatgctt tatgatgcgg aaggaaatat cggtcaagtg aaacgagact
    46681 tgaaactagt ctgtgcaaaa tcttttttac attaccgtac tcatcatgag aaaaagctaa
    46741 tcattcgaaa agaagaactt tcccttcaag tacaaaaagg attactgaaa atcaaggaaa
    46801 taccggaacg tctcgaccga ttcatggaca gcaaaagtca gtatttgaca tttgaaccag
    46861 gatttgctga tgttgtctgg tcacaagatc cagaacgaaa catgcaggtc tataacgata
    46921 ttgaggaaaa agtcacttct ctttccgaaa ccggtgtgga gaacattgat ctagagaccc
    46981 tcatttcaaa agatgtggat gcttatttcc agacatatgt caaagaattg actaaaagtt
    47041 cgattcccaa agatcttttg ccagatgaaa tctggcggtt aaccaatcaa ttgtatgatg
    47101 tggctgaaaa acggttagac cgtatttata atgaaaaagc tcgttttgct tttgctttgc
    47161 atctgcaaag tacattggat cgtgtgagag aaggtcatat gatcgtccac ccagatttga
    47221 ataacgtacg gaaaaatttt aagagggaat tccaggtagc aatcgatctt tcaacgatca
    47281 ttgaagaaga agaacaagtt gagattcctt ttgatgaaat cggctttatt agtatgtttc
    47341 tttctatcaa tctaggagaa agtgagcctg ttaaagaaaa caaagtagat gttctcatat
    47401 tgatgcacgg cagacagact gcttcaagca tgttggagat ggcacaagaa ctattaggaa
    47461 caaaagtagg gaaagctttt aatatgccac tggttatgga agttcaaaca atgtatgaag
    47521 aagtgttaca atatgtcaaa aactatcaag agcatttgac gaatggccta ctcttgttaa
    47581 cagatatggg atcattaaac accttcagtg atttgattta tgaagagact ggcattcgga
    47641 cgaaagctat cacgatgaca agtaccatga ttgtgataga agcgatacgg atggcaagtg
    47701 ttggaagaag cttagaagat atttatcaaa atatccagct ttcctttgaa tcgatcgtac
    47761 gtgatcagtt caaagcggaa aagtctgaaa ctttacagcg taaaaaagcc gtaatcgtga
    47821 catgctttac tggtgaagga gtagctgcca aattatatca gcgtattatg ccagtgatta
    47881 atcaaaataa ggtagaactg atccagatgc agtttttaga acgtgaatca ttcaaaaaac
    47941 acatcgatgc cttattggaa gaatatgaga tcaaagcaat tgcgggaaca gttgatgttg
    48001 attatcaaaa tatcccattt ttttcagcct atgacgtttt tgatgatgaa aaactaaata
    48061 ttttaaagcg tatagctgat gatgagatcc cgacagctaa aattgtcgat tcacttgctg
    48121 gatcattaag cgatgtcttt tctattgatg aactgatccg ttcattgcaa aagacagttc
    48181 agcagatcca gaccgatttg tcgattatcg tggaacctgg agtagatact ggaatcgtta
    48241 tccatttagc cttcatgtta gacggattga tcaaacaaga aagccatcgg caatttgaga
    48301 aactgtctat tttctcaaaa atttatcgtt tggaaatgga tatcgttcgg gcaaacttga
    48361 tggcactaga acgaaaatat caggtaaaaa ttccagaaga tgatatcgca tttcttacac
    48421 aaatgtttct tgaaaataaa ataaaaaaat gaacacacta atcatactgt ttacacacta
    48481 aacatctgtg tgcgcactat aaaaaagagc taaaaagcat gaaaacgcta tttcagctct
    48541 tttttttatt ggcatgcttc ttgcgttata taaaagtgta aaaaaataaa aggagtgtta
    48601 aaaatggcaa aaaaaacaat catgttagtt tgttcagcag ggatgagtac gagtttattg
    48661 gtcacaaaaa tgcaaaaagc agcagaagaa aaagggatgg aagccgatat ttttgcggtc
    48721 tctgcctcag atgcagataa taatttagaa tctaagaatg tcgatgtatt attattaggt
    48781 cctcaagtac gtttcatgaa agctcaattt gaacaaaaac ttgcgcctaa aggtattcca
    48841 ttagatgtca tcaatatgca agactatggc atgatgaacg gagaaaaagt tcttgcacaa
    48901 gcagaaaatc taatgaaata aatcaagtat gcagttgggg gtataacaac gtggaagatc
    48961 aacaaaatct agaagccatc atggggttaa tcatgtttgg cggtaatgca aagagcgatg
    49021 caatggaagc gattgcggct gctaaaatcg gtgattttga gttagcggat aggaaaatcg
    49081 ctgatgcaga agaatcactt atacaagcgc atcatgcaca aacagacatg ttgacacaag
    49141 aagcacaagg aaatcatatg caagtgacat tattgactgt tcacagtcaa gatcacttaa
    49201 tgacatctat tgcgtttact gatttagcaa aagaaatcat cgaactttat cgtcgcgtat
    49261 acagcgaata aatacaactt cttttttgaa cggaagaaga aaaatttttg aacggatttt
    49321 ttattttgtt ataaggagaa ctctctttat ataaaaaact tataggagga agtaaaaaat
    49381 gaacggacta tcaagttgga tggagaaata catcctgccg gttgccgcaa aaatcggagc
    49441 gcaaaaacat ttagttgctt tgcgtgatgc ttttatcggc atactgcccg caacaatggc
    49501 gggatcagtc gctgtaatga tcaatgcaat tattcgtgat ttaccgcaac agtttaacag
    49561 tacctatgct gacagtttag cagcagacaa aggaatcttt gccgtattga atgtcatcat
    49621 cggaatcaat ggttttgtct ggaacggtac actagcaatc gccggattga tctttgcctt
    49681 ttcttggggg tataatcttg cccgagctta tggagtaaat gatctagctg gggggatcgt
    49741 tggtttagct accttgattc aaggtatttc ctttggtcca gcattatcag cagcattaga
    49801 tgtgaatgtt ccttcaaatg tcgcagatgc aatcaatagt tctgagattg gtgcaacgat
    49861 tgcagatggc acactgtcag ttggattatg gggttggcta aaactagatc atttgaatgg
    49921 aaacgcttat tttacagtta tgatcatggg agctatttca gtcattattt tttgtaaatt
    49981 gatgcttgcc aacattacga tcaaaatgcc tgattcagta ccaccagcag tatctaaagc
    50041 atttgcagcc attttaccag cgacgatcgc tttatacgtc attgctatta tcaattatat
    50101 cgtaggtcgt gtagctgatg ggcaactgat cattgatttg gtgcaaaaat atattgcaga
    50161 accattccta ggattatctc aagggttggg agcagtattg atcgtcacag tatttgttca
    50221 agtattctgg ttctttggta ttcatggatc aaatgtattg gcaccagtgc tagagggaat
    50281 ctggggacaa gcacaactaa tcaatattga tattttccaa aaaggttata acggaaaaac
    50341 tggtacacca gccgtgttag atgcaatcga agatggaaaa gcttatatgt gggtccgcgg
    50401 atcgttcgat gcttttgcat ggtttggcgg atcaggcgga acgatcgtct tactgatagc
    50461 tatcttgatt ttctctaaac gtgcagatta tctaacagta gctaaattat ccattgggcc
    50521 tggtatcttc aatatcaacg aaccagtgat gtttggtctg ccaatcgtat tgaatgcaat
    50581 catgtttatc ccattcttga ttgctccagt agtagcaaca gcaattggat ggacagctac
    50641 ttatcttggc ctagtcgctc cggtatcgca gcaagtcaca tgggtagtac cgccgttctt
    50701 attgtcattc ttagcaacag gagcggattg gcgggcaccg atcgtaacat tagtatgtat
    50761 ggtcgtgact ttcttgattt gggcaccatt tgtcatctct gcaaacaaaa tggaccctgc
    50821 cgcaggcgaa caacaataag atgaacgtga ggagcgggac gctttaagtt tcgttcctca
    50881 taatgaattt tcgaggagga actgaaatga ttacaattgc cagtgcaatg caacaagatg
    50941 aaatcaaaga attgttagaa ggatacaatg aagaaggaat gacttttaca tttaaagaaa
    51001 aacaaggaat caagctgttc tttgaaacaa gtgcagaaga tccggaagtt gcggctaaaa
    51061 aagcaaagga actaatcaaa gcacagcctt ggggaacagt cctttatttt caatcaacag
    51121 ctgtttagaa aatggtttaa aaagatagga tggttgagat gataaaaaac ggtttatttt
    51181 tatgtagtat tggttctttg atcgtgttgt atagtttgaa tccacattcg caaaggtatg
    51241 atttgtatag tatggtcaca ggtctttttc taacaggatt aggaatctat ttcttcatga
    51301 aggggaaaaa aagagaagaa gcaaaggaga aaaataacta atggaaatca cgaaaaaatt
    51361 aaatattcca gcatcgtttt tttacgataa agttatggac tctgtccttt tcgatgtacg
    51421 taaagcgacc ggcaaatcag taactagaaa acagctgaaa aatttggagt acgtcaaaca
    51481 attctcgcaa aataaccggg ctcgcataaa aatcgaagaa gtcattgaaa accagtctta
    51541 taagtttcgt acatcgacaa cgaagaatga attcgtcgtt cattatcaaa tcaaaccatt
    51601 ggacgattcg acctgtgagg ttcattacac agaaaaaatg gagtcttatg gcatgcttca
    51661 aaagttgaat gatatgcttt taggaacaat cttgatgtat tttaaacgga gacgtttcaa
    51721 aaaaatgcta gatatgatcg aagcaacgta ttaaatcaat gaaacatggt actatttagt
    51781 tattgaaaaa tgaagtaagc tagaaggaat ttttcatagg aagatagatc tttcctgcga
    51841 atcctcacta gcttttttgt aggaaggggc agcaaaatga caaacaaagt aagagagcta
    51901 ggtgtatctt cgattgagga aatgtttgat ttagccactt atgcatttaa tggggcagat
    51961 acacctgaaa ggcgagaacg ttttcgtaca cttgcggaga attcttggaa ttacggattt
    52021 tttgatgagg aggatcggct aacaagtcaa gtcatggcga ccccttttcc agtgaatttt
    52081 tacggacaag agtacctaat ggccggcatt ggctatgttg cttcttatcc agaagcaagg
    52141 ggacaaggcg ggatcaatca gatcatggaa aagatccttg aagattgccg tgatcgtaaa
    52201 gtaagcttat cttatctggc gccattctcc tatcccttct atcggcgcta tggctatgaa
    52261 caatcatttg ataaaatcag ttatcaacta aacagtcgtg atgtgccgtt tatcaaaaaa
    52321 aaatctggaa aaataaagcg tatggatttt gaagatgcaa aagctgcaat cagaagaatt
    52381 tatgaacaga tgccggaaaa ccaacgagct ggtctgaagc gcgaagactg gtggtatacg
    52441 tataaattca agcaaaaagg caattaccag tttgctttgc attttgatga taatgaggaa
    52501 gcaacgggat atatcgttta tcagcttgca acgtcttcct ttattattgc ggagtgggga
    52561 tttttggatc atgatgcttt ttgcagcctt gttcgcttcg taagttctca caatggtgct
    52621 tttgaaacat ttgaatattc atgcggctac ggcggtaatg atcaaaatta tctattagaa
    52681 aatccatttg cttctgtaat gatcacacct tatatgatgg cgaggatcgt agatatttct
    52741 ttgtttttgg aaaattatcc atttaaaaag agacaagctg catttgccct acaaatagaa
    52801 gaagatcatt atgcgaaatg gaatgaagga atcgtggaag taacaatcga aaatggaaaa
    52861 aatcatctct caaaagtaga acaaacaaaa ctcccaatcg ttcgaggaag tatccaacat
    52921 atcacgcagc tattattagg gtatcgtaaa gtggaagagt tggtattcca agaaagaata
    52981 caggtgacaa aagaatatga agaaacgtta agctcaatct tgccacagca acagccaatt
    53041 cttaatgact atttttgata aaaaacgggt gatgacaata tttttgtcac cacccttttg
    53101 tttttcattt ggcatttgaa ggaaaatgtt aaagtaaatc ctcttttttc gtaaatttga
    53161 attttttgtt ttcttttgtt ttagggtttt cttttttgtg gtcttggatt gcttctttcg
    53221 taatcttttc ccgatttttt ttagatgaat ctaagcgttc tgcgtatact ttttcttttg
    53281 gcttatcatt cattggaaag gacctcccta cattatttca ttctacctct agtttatcat
    53341 ctgttaagga ctagtggaaa cgttttgcct aacttataag ctgatttttt tagcagataa
    53401 gcttgcaagt aaaaaacaaa caagctacaa ttgatttatt gaaattgttc ctgtagctca
    53461 gtaggataga gcaatcgcct cctaagcgat aggtcgctgg ttcaattcca gtcaggaacg
    53521 tataaataac ccctgtatcc tttgcggtac aggggtttgt tttgtatttg tgccaaaaat
    53581 gtgccaaact agttatcatt tttatataaa ctatcgaaca cagtattgat tttttcttta
    53641 tcctgatctt caagctctcg aacaatatga agataagtag acattgtagt ttctaaactg
    53701 ctatgtccta aacgtttcga tacgctaagt atatttactc cttgataaag taaaatagat
    53761 gcatgtgtat gccttagtcc atgcaaagta aattgctttt caatccctaa ttcttttaat
    53821 tttttgcgca aatatttgct gacggcgttt gatgaaacta atccattttt taagttgaaa
    53881 aaaacaaaat tatgagggtt tttgatttca aaattctcga ataattcttt ttgattgatt
    53941 ttaaactttt ttaagagatc aatcgtatgt ttatcaatgg aaattttcct atttgaagtt
    54001 tcgttttttg tttttcccca ttcatttaat ttataattcc atgttttatt aatagtgatg
    54061 atttgatctt cgaaatcgat atcttcccat gttaatccta gaagttcagc aaatcgcatt
    54121 ccagtggcac cagcaactaa cacgagcata ggagaagagt actgcgcaga aaggttttct
    54181 tctacgagtt tcattaaaga tttaaattga tcataatcta aatatttatc ttcttttggc
    54241 ttcaatgaat cttttccttt gattactgct tttctagttg gatcaaacgg aattaatcct
    54301 tcttctacgg catctttcaa cgacgctcta atatggttat taaacttaat aacggtagat
    54361 tttacatggt ctttagcata cttatttaaa aagcgttgat atcccactct atctaaatca
    54421 gaaatcaata ccgctggcat atatttcttt atgttcatta acgtatcttc gtattttcta
    54481 taagtgatag gagatactgt tccttcttta taaagttgca tccaatcctc aaagtaatct
    54541 gataataata gatttttgcg atccccttta aggcctttgt tcaagttata ttctaattcg
    54601 ttggcggcat cttttgcttc tccttttgtc ttaaatcctg attttcttat cttcgtgtat
    54661 tttccgtcat cttttttgta tgaaatttcg tattgccaac tatttccacg tttgactaat
    54721 cttgccataa ttgaatcagc tctctttctc tgatacaata ggaactataa agaagcccat
    54781 tgtttgggtt ttattatttt ttgcacatcc tttttgcttg ccggcgcaag gatgtgctat
    54841 ttgtcgtttt aatggtataa ttggatgtgt attagatcat ccaatcagag gtgtttatta
    54901 tgattaaagt gatttttaaa agaataactg aacttccaga gtttagatta tttgttgatt
    54961 attggtatgt ctggtttttt attgttgcat tgctgatagg tctagatgtc atattagaaa
    55021 gaaaacttaa gtaagagttg agttaataaa ttgaattcct aaatgagata ttttgtagcc
    55081 catattttgt ttgccgacat cacctaatac tatggagcta atcgtttttt ctaacagacc
    55141 aaaatcagat aatctattta agctaccatc tattagctct tgtgaatagc cagacatgct
    55201 gatatttcca gtgaaatttt cgtttccacg actcattaag catgctaaca attgaatctc
    55261 aatcgatgtt aattgattca gtacaagaag ataatactcc tcgttatccc atgaagcgtt
    55321 gttaattcga agtaaacaat tcttataaag atttttatac atatctattt tattttgtgc
    55381 ctttgatttt tctacctcgt catttatccc ttcgaatatt ccaatgagtt ggtccttgtt
    55441 ctctaagttg aaaacatctt ctggaatttg atcgcgaatg ttttctaatg attcgtttaa
    55501 atttttgtag aatttctcga tgcgtttgaa acgcttctcg tttttagacc caaaataaag
    55561 tgactgcaca acgccaccaa tataaggaat tgctccaata ccgccttcaa ctattaattc
    55621 aagatattct tttttggtta gtttttcatt gctattatcc atagttacct cctattttgt
    55681 catttccttt acaagcattg tgactcctga tggcactctt ttgtataggt tttattattg
    55741 tgcacaatct aactcttagt ggggcgtggt tcatttattt tttacttact ttactattaa
    55801 tcatatccca ttgagcatag gatttttcta tgttcgatcc tcttgaacta atttctcctg
    55861 taaaagttac tttatcgccc tttgtaaagg tatcagcatt gtttaagtcc ttcgcaaaaa
    55921 ttacatatga aactttaggt gttgcagaaa tatcattcca acttttatca tcgtactctt
    55981 tgtctgctat aatggctatt ctagtagtca ttgattcgat aacagttcct tcaaaagtat
    56041 atgttttacc actaatatat ttagtaaaag ctgtattttg ctcagtagct ggagtaatag
    56101 aataataact atctaagaaa gcaccccagt catgattatt tgtctctaaa aattcttcaa
    56161 attgagttag gcttttattg ctgctgttag atgtagtagt ttgagtatca gatgatgagc
    56221 tttcctgttt gttattgtca tttgcctcgt ttgatgagga aagcgagaaa cctacggtag
    56281 taaaaataat cccgataata aggagaatca taggtgtttt cttatttgtt tttttgataa
    56341 atcttacaat taacatgatt aaacctataa taaataaaac aataccagta aaaccaaata
    56401 taccgtccat taatagatcc tctttctctt ttgatattga atattataaa ccaagcaatt
    56461 cttttttctt aaaatcgaat tcttcttgat taataatccc ttcatctaac aactctttat
    56521 actttttaat ttcatctgca cttgaaattt gtgtttcttt ggaattatta tctaatatag
    56581 attgttgctt ctctatatat ttttttaaat tttccatttc attccaatat ttttttgtaa
    56641 acataacggt gttttcatct ttcgtggccg ctaatatacc tccgcgactt tcgtttccgc
    56701 ctagcaaggt aaattgtata taaccattac tcatacctgg tttttttaat tgtacggctg
    56761 atatattttt aaaggggat
//
LOCUS       DADVXU010000007        55419 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.007, whole genome shotgun sequence.
ACCESSION   DADVXU010000007 DADVXU010000000
VERSION     DADVXU010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 55419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.007"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            137..994
                     /locus_tag="KB953_000426"
     CDS             137..994
                     /locus_tag="KB953_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HBB6865428.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            1109..2029
                     /gene="pstC"
                     /locus_tag="KB953_000427"
     CDS             1109..2029
                     /gene="pstC"
                     /locus_tag="KB953_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HBB6865429.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            2029..2913
                     /gene="pstA"
                     /locus_tag="KB953_000428"
     CDS             2029..2913
                     /gene="pstA"
                     /locus_tag="KB953_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBB6865430.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2928..3734
                     /gene="pstB"
                     /locus_tag="KB953_000429"
     CDS             2928..3734
                     /gene="pstB"
                     /locus_tag="KB953_000429"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBB6865431.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3750..4508
                     /locus_tag="KB953_000430"
     CDS             3750..4508
                     /locus_tag="KB953_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBB6865432.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGINLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4521..5198
                     /gene="phoU"
                     /locus_tag="KB953_000431"
     CDS             4521..5198
                     /gene="phoU"
                     /locus_tag="KB953_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBB6865433.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5436..5735
                     /locus_tag="KB953_000432"
     CDS             5436..5735
                     /locus_tag="KB953_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865434.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGRLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKK"
     gene            5761..6468
                     /locus_tag="KB953_000433"
     CDS             5761..6468
                     /locus_tag="KB953_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HBB6865435.1"
                     /translation="MSIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLL
                     NISILFVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYE
                     ELNYLTRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQV
                     GRDVIGSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVL
                     SIPISVLFFLTVRRLKR"
     gene            6465..7232
                     /locus_tag="KB953_000434"
     CDS             6465..7232
                     /locus_tag="KB953_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HBB6865436.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7332..8900
                     /gene="liaX"
                     /locus_tag="KB953_000435"
     CDS             7332..8900
                     /gene="liaX"
                     /locus_tag="KB953_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HBB6865437.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEITQVQL
                     STTTGSIYLKDTDK"
     gene            9197..9517
                     /locus_tag="KB953_000436"
     CDS             9197..9517
                     /locus_tag="KB953_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HBB6865438.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            9517..9867
                     /locus_tag="KB953_000437"
     CDS             9517..9867
                     /locus_tag="KB953_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBB6865439.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            10034..10972
                     /locus_tag="KB953_000438"
     CDS             10034..10972
                     /locus_tag="KB953_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HBB6865440.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10987..11817
                     /locus_tag="KB953_000439"
     CDS             10987..11817
                     /locus_tag="KB953_000439"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBB6865441.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11861..12886
                     /locus_tag="KB953_000440"
     CDS             11861..12886
                     /locus_tag="KB953_000440"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBB6865442.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12901..13839
                     /gene="galU"
                     /locus_tag="KB953_000441"
     CDS             12901..13839
                     /gene="galU"
                     /locus_tag="KB953_000441"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBB6865443.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKKLEAEGSKIDHNPKGITEIKEEKKK"
     gene            13964..14404
                     /locus_tag="KB953_000442"
     CDS             13964..14404
                     /locus_tag="KB953_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HBB6865444.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14407..15009
                     /locus_tag="KB953_000443"
     CDS             14407..15009
                     /locus_tag="KB953_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBB6865445.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDTKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(15077..16183)
                     /locus_tag="KB953_000444"
     CDS             complement(15077..16183)
                     /locus_tag="KB953_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBB6865446.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLASIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            16393..17394
                     /gene="ccpA"
                     /locus_tag="KB953_000445"
     CDS             16393..17394
                     /gene="ccpA"
                     /locus_tag="KB953_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HBB6865447.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(17697..20042)
                     /locus_tag="KB953_000446"
     CDS             complement(17697..20042)
                     /locus_tag="KB953_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBB6865448.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            20321..21223
                     /locus_tag="KB953_000447"
     CDS             20321..21223
                     /locus_tag="KB953_000447"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBB6865449.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            21436..23211
                     /locus_tag="KB953_000448"
     CDS             21436..23211
                     /locus_tag="KB953_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HBB6865450.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSFYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYSSSKKMLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYSIAAIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHAVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFAFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTAFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            23468..24685
                     /locus_tag="KB953_000449"
     CDS             23468..24685
                     /locus_tag="KB953_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBB6865451.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEENTLCSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFLNRLTPYALILIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKEALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(25132..26718)
                     /locus_tag="KB953_000450"
     CDS             complement(25132..26718)
                     /locus_tag="KB953_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HBB6865452.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
     gene            26969..27193
                     /locus_tag="KB953_000451"
     CDS             26969..27193
                     /locus_tag="KB953_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HBB6865453.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            27335..29086
                     /locus_tag="KB953_000452"
     CDS             27335..29086
                     /locus_tag="KB953_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBB6865454.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLAPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAALLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGCSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQKLEAKIEGGEA"
     gene            29086..30870
                     /locus_tag="KB953_000453"
     CDS             29086..30870
                     /locus_tag="KB953_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6865455.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPTITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLTKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            31328..32179
                     /locus_tag="KB953_000454"
     CDS             31328..32179
                     /locus_tag="KB953_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBB6865456.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELYQVIQKKYKDTFGCVEKIADFLAEIFNYQMSTDEKMYLTIHIARL
                     VHENRCK"
     gene            32276..34162
                     /locus_tag="KB953_000455"
     CDS             32276..34162
                     /locus_tag="KB953_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBB6865457.1"
                     /translation="MGKYQELASKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGVVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTALLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPAFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNPGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEAGETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVTNYTDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(34203..35105)
                     /locus_tag="KB953_000456"
     CDS             complement(34203..35105)
                     /locus_tag="KB953_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBB6865458.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            35335..36738
                     /locus_tag="KB953_000457"
     CDS             35335..36738
                     /locus_tag="KB953_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBB6865459.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMNELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            36735..38171
                     /locus_tag="KB953_000458"
     CDS             36735..38171
                     /locus_tag="KB953_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBB6865460.1"
                     /translation="MMGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFP
                     VITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGD
                     EKEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENL
                     CRVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASA
                     IATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPA
                     YALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFAS
                     VKNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDY
                     RIDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGN
                     GTLARSKKKSFNWYKKVIDSNGENLSND"
     gene            38316..39242
                     /gene="trxB"
                     /locus_tag="KB953_000459"
     CDS             38316..39242
                     /gene="trxB"
                     /locus_tag="KB953_000459"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBB6865461.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            39455..41182
                     /locus_tag="KB953_000460"
     CDS             39455..41182
                     /locus_tag="KB953_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBB6865462.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     TIIQKMTEE"
     gene            41198..41548
                     /locus_tag="KB953_000461"
     CDS             41198..41548
                     /locus_tag="KB953_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBB6865463.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAIIQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(41632..42492)
                     /locus_tag="KB953_000462"
     CDS             complement(41632..42492)
                     /locus_tag="KB953_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HBB6865464.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPSVEFNLFRRT
                     FVTDEKTLMQALQDALEEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            42584..43012
                     /locus_tag="KB953_000463"
     CDS             42584..43012
                     /locus_tag="KB953_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBB6865465.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            43174..43350
                     /locus_tag="KB953_000464"
     CDS             43174..43350
                     /locus_tag="KB953_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBB6865466.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            43377..43823
                     /locus_tag="KB953_000465"
     CDS             43377..43823
                     /locus_tag="KB953_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HBB6865467.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(43979..45358)
                     /gene="brnQ"
                     /locus_tag="KB953_000466"
     CDS             complement(43979..45358)
                     /gene="brnQ"
                     /locus_tag="KB953_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBB6865468.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            46064..47035
                     /locus_tag="KB953_000467"
     CDS             46064..47035
                     /locus_tag="KB953_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBB6865469.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            47059..49251
                     /locus_tag="KB953_000468"
     CDS             47059..49251
                     /locus_tag="KB953_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HBB6865470.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            49268..49744
                     /gene="ybeY"
                     /locus_tag="KB953_000469"
     CDS             49268..49744
                     /gene="ybeY"
                     /locus_tag="KB953_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBB6865471.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            49728..50123
                     /locus_tag="KB953_000470"
     CDS             49728..50123
                     /locus_tag="KB953_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HBB6865472.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            50138..51037
                     /gene="era"
                     /locus_tag="KB953_000471"
     CDS             50138..51037
                     /gene="era"
                     /locus_tag="KB953_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBB6865473.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            51180..51992
                     /gene="recO"
                     /locus_tag="KB953_000472"
     CDS             51180..51992
                     /gene="recO"
                     /locus_tag="KB953_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBB6865474.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            52376..53293
                     /gene="glyQ"
                     /locus_tag="KB953_000473"
     CDS             52376..53293
                     /gene="glyQ"
                     /locus_tag="KB953_000473"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBB6865475.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            53295..55370
                     /locus_tag="KB953_000474"
     CDS             53295..55370
                     /locus_tag="KB953_000474"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBB6865476.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRASRGHRFLGEDVTFTHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT        17958 a         9935 c        11786 g        15740 t
ORIGIN      
        1 agcatagaaa cttttaatga gatagttttt tttacaatta tgagaattta ttttgttatt
       61 taatgatttt gattcgtttt catcaagtaa tgaaaatgat aaaataaaca tactttagga
      121 ttaaaggaga tttctaatga aaaaacttaa attattctta ccaataatag gattagccat
      181 tcttttatca ggctgttcaa agtggattga tcgaggagaa tccattacgg ctgttggctc
      241 ttctgcttta cagcctttag tagaaacagt tgctgaaact taccagacag aaaatcctgg
      301 aaaattcatc aatgtacaag ggggcggaag cggaactggt ttgtctcaag tacagtcagg
      361 agcagtcgac atcggaaact ctgatttgtt tgcggaagaa aaatcgggga tcgatgcctc
      421 agcattagtc gatcatcgag tagcagttgt tgggatcact cctattgtga acaaggatat
      481 tggcgtaaaa gacatcagta tggaaaacct gaaaaaacta tttacaggcg agatcaaaaa
      541 ctggagtgaa gtcggtggaa aagatcagcc agtagttata ttgaaccgcg cttcaggaag
      601 cggaacccgt agcacgtttg aaaaatgggt attggacgga gaaacagctg ttcaggcaca
      661 agaacaagat tcaagcggaa tggtgcgcca gatcgttgcc gacacacccg gtgcaatcag
      721 ctatgttgct ttttcttatg tttccgatga tgtagatacg ttgaatgtta acggtgtaga
      781 accgacagat gaaaatgtga caacgaacaa atggacgatt tgggcatatg agcatatgta
      841 cacaagagat catccaaaaa aactgacaaa agaattttta gactatatct tatctgatga
      901 agtacaagat aacatcgttg gagaactagg ttatatccct gtttctcata tgaaagtcga
      961 acgtgattgg caaggaaata tcgttaaaga gtaatcttta cacaaaattt acaaacttag
     1021 ctaaactttt acatgttatt aaaaccaaat tgatgttcaa tttgtacact gagtgagtat
     1081 gttaaacctg attaaggggg agtcatctgt ggaagatgtg aaaaaagtat tattaacaaa
     1141 atcaaaacgt tcaaaaatgg aacagcgtgg aaaattcatc agttttttat gtatcgcgct
     1201 gatcgtgttg gttgtattat ctatttttta ttttgtagct agtaaaggat tggccacctt
     1261 ttttactgat aagatcaatg tatttgattt tctgtttggt acagattgga atccaagcca
     1321 aatcggagca aatgggaaac caatggtagg ggcattgcct atgatcacag gttcgtttat
     1381 tgtgaccttt ttatctgcta tcgttgccac gccatttgcc attggtgcag cagtattcat
     1441 gacggagatc tctcctaaaa acggaacgaa gatcttacag ccggtcatcg agctgcttgt
     1501 aggtattcct tctgtcgttt atggttttat cggtttatca gttatcgtgc cttttgtcag
     1561 aagtattttt ggcgggacag ggtttggtat cttagctggt acatttgtgt tgttcgttat
     1621 gatcttgccg actgtcacaa cgatgactgt tgatgcatta aaggcagtgc cacggcacta
     1681 tcgggaagct tcattagcgc ttggtgccac aagatggcaa acaatctaca aagtcgtgct
     1741 acgagcagcc gtgcccggga ttttaacagc agtcgttttt ggtatggctc gtgcgtttgg
     1801 tgaagcctta gctatccaaa tggtcatcgg taatgcagca ttgatgccaa caggacttac
     1861 tactcctgct tctacattga cgtccatctt aacaatggga atcggaaata caatcatggg
     1921 aacagttgaa aataatgtgt tatggtcatt agcgctgatc ttgctattga tgtcattatt
     1981 ctttaatatc gtgatccgta tgattgggaa gaaaggagag ctgaaataat gaacgcaaaa
     2041 cgctcagata aaattgctac aggtgtttta tatatagttt cggggatcat cgtattgatt
     2101 ttagcagctt tactacttta tatcttagtt cggggaatac cgcatatttc atggtccttc
     2161 ttgaccgaac cttccaaagc ctaccaagaa ggcggtggaa tcgggatcca gctatttaac
     2221 tcgatttatt tgcttttgat cacgatgatc atcagttttc ctatttcttt aggtgcgggt
     2281 atctatcttt cagaatatgc tggaaaaaac tggctaacag atgtgattcg cacgtcgatt
     2341 gaaatcttga gctcgttgcc ttctgtcgtt gtcggtttat tcggtttctt ggttttcgtt
     2401 attcaattcc agtatggttt ctcgattatt tctggtgcat tggctttgac tgtattcaac
     2461 ttgcctcttt tgactagaac agttgaggag tctttacaag cggtccatta cacacaacga
     2521 gaagcaggat tggccttagg tttgtcacgc tgggaaacag tgataaaagt ggtggttcca
     2581 gaagcgacac cagggatttt gactggtgtg atcctaagtt ctggaagaat ttttggtgaa
     2641 gcagctgcat tgatctatac ggctggacaa agtgcacctg cgttagattt tacgaactgg
     2701 aatccattaa gtgtttcaag cccgatcagc attttccgtc aagctgaaac attagctgtc
     2761 catatttgga agatcaatac agaaggcacg atgcctgatg gtgtttctgt ttcggctggc
     2821 gcttctgccg tgttgatcat tgctgtttta ctgttcaact tcggcgctcg tgcgattggc
     2881 aaacgattat accgaaaaat gacttcagcc taaggagaag aagcaaaatg aaaaaatata
     2941 actggaatga aacaaatgtc atcaccatgc caaaagagaa agaagtagca ctatatacgg
     3001 atgatctaca tgtctggtat ggcgacaatg aagcaatcaa aggagtggat ctggaatttg
     3061 aaaaaaataa gatcactgca ctgattggtc cttccggctg cggaaaatct acgtatcttc
     3121 gttcgttgaa tcgaatgaat gatgggatcg ctaatacgaa aatcactggg aaaatcatgt
     3181 atcaaggcgt agatattaat gcgcctcaag tggatgttta tgagatgcgc aaacggattg
     3241 gcatggtttt tcaacgtcct aatccgttca gcaagtcgat ttatgacaat attacatttg
     3301 ctttgaaaca gcacggcgaa aaaaacaaaa agaaattaga tgaaatcgtt gaaacaagct
     3361 tgaaacaagc tgccttgtgg gaccaagtaa aagacagctt ggacaaaagt gccttggctt
     3421 tatctggcgg gcaacagcaa cgtttatgta ttgctcgtgc gatcgcgatg aaacctgata
     3481 tcttgctgct agacgagccg gcaagtgctt tggaccctat ttcgacagga acggttgaag
     3541 aaacacttgt aaacttgaaa gaagactata cgatcatcat cgtgacgcat aacatgcaac
     3601 aagcagctcg tatcagcgac tatactgcct ttttctatct aggaaaagta atggaatatg
     3661 acaaaactaa caagatattt accagaccaa aaattcaggc aaccgaagat tatgtgtccg
     3721 gtcattttgg ttaagacgga ggcctcaaca tgacaaaaga aattatttca tctaaagatc
     3781 ttcatctgta ttatggaaag aaagaagcac taaaagggat caatctaagt ttcaatcaag
     3841 gggaaatcac tgcgatgatc ggtccttctg gttgcgggaa atccacttat ctgcgttctt
     3901 tgaaccggat gaatgacctg attccgaatg taacgatcac cggaagtgtt ttatataaag
     3961 acaaagacat ctatggtcca aaaacagatg ttgttgaact aagaaaagaa atcggcatgg
     4021 tattccagca gccaaaccca tttccttttt ctatctatga aaatgtcatc tatggattga
     4081 aattaaaagg ggaaaaagat aaaaaagtcc ttgatcaagt agttgaagaa agcctgaagg
     4141 cagcgtctgt ttgggatgat gtgaaagaca aattgcataa aagtgcttta tctttatccg
     4201 gtgggcagca gcagcgtgta tgtatcgcac gtgttttggc agttaatccg gaaatcatct
     4261 tattggatga accaacaagt gcgcttgatc ctgtttctac tgggaaaatc gaaagtatgc
     4321 tgttagaatt aagagagcgg tatacgatga tcatcgtgac ccataatatg tctcaagctt
     4381 cacgtatttc agataagacg gcattctttt tagatggtca cttgatcgaa tacaacgata
     4441 cgaagaagat attcatgaat ccagaaaaac aagagacaga agattatatt tcaggtcgat
     4501 ttggataagg aggaaacgga atgctacgta cacaatttga agaagaattg ctgaatctcc
     4561 ataatcagtt ctacgaaatg gggatgatgg tgagcagcgc cgtgcacaag tcagttcgtg
     4621 catacatcaa acacgataaa atcttagccc aagaagttat cgaaaacgat ataaatatca
     4681 ataacatgga aacacgcttg gagaagaaaa gttttgaaat gatcgcattg caacagccag
     4741 ttacaactga tttgcgtatg atcattacgg tcatgaaagc tagctcggat ttagaacgta
     4801 tggccgatca tgcggtgtct gttgctaaat ccacgattcg cgtaaaaggg cagacgagaa
     4861 taccggaaat cgaaaaagag atctccgata tgtccgatta tgtgaaaaaa atggtggaca
     4921 acgtcctagt tgcttatgtc aaaacagacg aaaaagacgc acgtacgatc gccaatatgg
     4981 atcaaagagt caatgactat ttcaatcgaa tctataatgc aacaatcaaa aatatgcagg
     5041 aaaatcctga gacagtcatc agtggaacag actacttgaa tgttgccagt tatctagaac
     5101 ggatcggaga ctatgtgaca aacatctgtg aatggatcgt ctacttggct acagggaaaa
     5161 tcactgagtt aaatacgaac cacaatgaga ttttctaaac tgaagctgtg attatgccgt
     5221 ttgtttagga aaagaggttg tgaaatcagc ggttgtgaaa aagctgttct gcatcaagta
     5281 ataagaacag tcaaacaaaa aatagctcct catatttttc gtttggctgg acttattacc
     5341 gcagatgcaa gcttttgaac accgtttatt cgggaaaggg tttgtgacaa aacatgtcac
     5401 aaaccctttt catcatttca tagaaaggat aaagaatgca aaaaaaaatt agcatcatca
     5461 ctatattttt tatttgtgtg cttacttcat gggttattca gcatcaagct gctttatatg
     5521 aagtacctgt catcgagatc gaaaaagtac gaataaaaga ggatcatcaa ttgattacag
     5581 gaagactgat gaacacggat caaaaaggag aatcgattta tttatcggtt ccttatcaaa
     5641 aaaatcaaat agaaacagca aatataaaag cgagacaaat gtatctagtc caaatgcatg
     5701 gagatgaatg gcagctagta gcaaaaaaaa aatgatggct ggcttttctt cttcacaagt
     5761 atctccattg gcgccatgtt gcttattggc ggaaaacagg gtctatttgc tttaggaagt
     5821 ctcatcatca atatcgtttt acttttactt ttgttgctca tctttactaa aacagaaggg
     5881 atttctctat taaatatatc tatcctgttc gtcgttatag ggatgattat tagcatattg
     5941 gctttagatg gatggaatag aagcagtata atcaaaattt cagctgcagt tacgagtgtt
     6001 tttttagcat tttttatttg tcttttgaca atgaatcatt ggaaagacca aggactgcgc
     6061 tatgaggaac tgaattatct gacacgaccg tatcgttctg tttttctttc tagcgtactg
     6121 attggaacag ctggaggaac actcgatacc gttatcacag tcattgcgac attagaagaa
     6181 ttgactaagc gacaaccaga aataacagcg aaacagttat ggctttcagg aaggcaagtg
     6241 ggtcgagatg tgattggctc gatgaccaat atccttttgt tcgtctatat cagtgggtca
     6301 gttccatcgc ttcttttata tttaggaaat caatggtcgt tcaaagaaac gatagaacag
     6361 catttatcat tggaattttt acgcgtcatt gctggcagct taggaatagt attatcgatc
     6421 cctatctcgg tcctcttttt tctgactgta aggaggctga aacgatgaat gtacttatta
     6481 tacttgttgg gattttagca atcagcgtgc tacttattgg cggtacgcaa ggtatgtata
     6541 ttcttttagg cttattcatc aatttaggga tctttttcct gttgttattc ggttatcatc
     6601 agaagtggcc gatactggca ttgtcggtca taggattttt attgattgct gtggttatct
     6661 tattttttat caacggttat aatctaaaaa tgcgtgcagc ttttgtgagt atactgatct
     6721 ttttattttg tttcctacta ttgcttccta tcactgattt tttagcgatc caaggtttta
     6781 catcggtaga actagaagaa ttatctggct tagataagac tttagcgatt gattttagat
     6841 tacttacgag atcgttgctt ctgattagtc ttagtggtgc tgttttagat gctagtgtag
     6901 caatttctag tgggacattc gaagtatatc aggcaaatcc gcaattatct ttttaccaat
     6961 tgcgccattc tagtttcgct attgctaaaa aaatattggc gtcaacagta atgacacttc
     7021 tttttgcatt catgggaagt tctttagcgt tgatcctctg gtttattgat ttagacattc
     7081 cttttcagca agtgatcaat gaaaaatcat ttgttctaga atatacgatg gctattctta
     7141 ccacattgtc tgcactactt gttcttcctt taacctgcgt gactacagca tatctcttta
     7201 caaaaaagaa agaacaactc aagatggagt aaaagttaca acttaagtcc tatgacattt
     7261 ttactgaatc agtgcataat atggatgtaa ggaaaagctt gcaaacaatt ttgcttaagg
     7321 aggcaatttc catgaaagaa agagaacgta ttttagattt agtgaaaaaa ggaatccttt
     7381 cgactgaaga aggcttagat ttattagaaa gcatggcaac tgaaaaagac gaaaagcaaa
     7441 taaaaaaaga agcagacaag gtaactgcta gtcataaaga aaaagatcag gcaagtcaat
     7501 tgatcgataa acttgaaaac ggcgaagaag aaatttctga gccagttgat ccaaaggaaa
     7561 gagaaaggca agatcaagaa aacttagaaa agatcttaga tgaactagca actgaagcga
     7621 ataaaacttc ggcgcgtcta gatgaagtaa atgctcaatt cgctgacaat aaggcagcac
     7681 gcaatgagaa acaggaagca ttgatgcagc tgaatacaaa agaagaatta ggcgagttga
     7741 cagaagaaga attagcacag cgtcagacgt tagaagcaga aatcaaagaa ttagaagcta
     7801 gtggcgatac actgttagaa gaacaattga aattagaagc agaattaaaa gatatccgta
     7861 agaatcaatg gtctgagaaa aaagaaacgt ttactgataa gtttgaattg ccagatgatt
     7921 ggaaagatca agcaacagat acactgaatc aagtaggcga aaaaatgtca gaagctggat
     7981 cacagttagg caaattcttg aagaaaacat tccaaactgt ttcagaaaca gtgaatgaca
     8041 acatggaatg gaaagatgtc agcttacgtg tccctgggat tgcgacaacg aaatttgagc
     8101 atgaatttta ctatgaagct cctgcagcaa gtatccttga tatcaaggca gcaaacggaa
     8161 acgtgacgtt aaaaacgtgg gacagcaacg atgtgaaagt tgaagctaag atcaaacttt
     8221 acggaaaaat gggtgctgaa ccatttgaag cattctctga acggagccag attgaggtta
     8281 atgaagatca tatctccttc caaattccaa ataaacgtgt gcgtgcagac cttgttttct
     8341 acttacctaa acgagtatac gaccacgcag ctattaaact tctaaatggc aacatcatga
     8401 ttgaaacatt ggaagctaaa gatatttata caaaatctac gaatggtaac atcatcgtgg
     8461 accaattaac tgcaacgatg ctggaagtag aaggtgtaaa cggcaatatc gatattcgaa
     8521 acgggaacat cttagattct attatcgaaa cagtaaatgg tacagtgaca tttggtgcga
     8581 cagcagaaaa cctttctgtg tctttggtaa atggtgatgt acgtcttacg attaaagaag
     8641 ataatttgaa aaaagtagaa gcaagttcag tgaacggaaa tgtcaaagtc gctttaccag
     8701 atggcatcgg tttggaagga catgctaaaa caagtcttgg aagtatcaac agccgtctgt
     8761 ccaattacga agtagttcgt gagaaaaaag aacggacaaa ccaaatgttg caattccgtc
     8821 gtgtttcaga tggtgaaatc acgcaagtgc aactttcaac gacaacagga agtatctatt
     8881 taaaagatac agataaataa agaggttgtg aaagagatgt tcagccttga gcaataagaa
     8941 gcgattttgt aaaatagctt cccctatttt acaaaattgt gccttattgc cgataggcta
     9001 tctcttgaac accgtttacc aagagattat gaacctgcca attatcgccg agtcacaagg
     9061 aataatttca gaaaaagctc gtcacgtttt atgaatatgg taatttgtga ccgagaattt
     9121 tgcagaaaga caccgattaa gaagaaggtt gtcacaacga ttttcttcat ttaaaatagt
     9181 agaacagagg tgagacatga tgaaaaaaaa gttgacaaaa tccaataaaa atgttgtttt
     9241 gacagggact ttagcaggaa taggagaata catcgggatt gatccgactg ttatccgtgt
     9301 gctttatgta ttcctgagct tagttgcttt tggaagtcca atcattttat acattttatt
     9361 agctttgatt atcccatctg cgcgttctgc acgaacaaac tatggacata acaatgcata
     9421 ttatgaaaaa aacaattatc gtgaacaaac tcgtgaacaa acaaaaaaac gcaaagaagc
     9481 ggaaaaaatc gatgatgacg aatggagtga cttttaatgt cgtactttca acgtatttta
     9541 gttaatacgt taacattcat atcattggct gttctttttc ctgcgatggt ccatgtgagc
     9601 agtatttgga cggccgttct tgccagcttc gtattgtctg tgttgaatat gtttgtcaaa
     9661 ccgattttga cgatactgtc cctgcccttt acattagtca cattgggatt gtttacgttt
     9721 gttatcaatg cagcgattct taaaatgacc tccttttttg tgggagcaga acattttagt
     9781 ttttcttcat tcggtgcagc gttgttagta gctatcttga tgtcgcttgt caatgtaatt
     9841 gtcagtgaac ataacagtag aaaataaaag aaagctgtgc cgaactatcg gcgcagtttt
     9901 tttatttaga atcaggaatg cgttttctat tacattggta attaaataaa aacgtttttt
     9961 ttaattggta gtacttttgg ctagacaatg ataaaatgat agagaacact ctaatagaaa
    10021 tgaggtagca tgaatgtcag aagttgtaaa agttagccaa ttagtgaaaa aattatcttt
    10081 ggaaatcgtg acaggcgacg agaaaagctt agaccgtgtc atcgtaactg gggacatctc
    10141 acgcccagga ctagagttga ctggttattt taattattat tcccataatc gtttacagct
    10201 atttggcagt aaagaaatca gttttgcgga acggatgatc ccagaagagc gtttgatcat
    10261 catgagaaga ttatgtagtg aagatatgcc cgcatttatt atttcaagag gattaccggc
    10321 tccagaagaa atgatccagg cagcaaatga aaatgggata gctgtactgc gttctcccat
    10381 ctcaacttct cgtttattag gagaattatc cagttattta gatggtcgcc ttgcccctcg
    10441 aacaagtgtc cacggtgttt tagtggatgt ttacggatta ggtgtactga ttcaaggtga
    10501 cagtgggata ggaaaaagtg aaacagcgtt ggagttgatc aaacgcggtc atcgattgat
    10561 cgcagacgat cgagtagatg tttatcagca agatgaatta acggtgatcg gagaaccgcc
    10621 taaaatcctg gaacatctaa ttgaaatccg aggaatcggg atcattgatg taatgaatct
    10681 ttttggtgcg agtgcggtac gtggctctat gcaagtccag ttggctgttt atctggaagc
    10741 atgggcaaaa gataagaaat acgaccgtct gggctctgaa gacacgaccg ttgaaatcgc
    10801 ggaagtgggc attccacaaa taaaaatccc tgtcaaaaca ggacgaaacg ttgcaatcat
    10861 cattgaagta gccgcgatga atttccgtgc acgtacaatg ggctttgacg caacgaaaac
    10921 atttgaagaa agattaagtc gcttgattga agaaaactca ggcaacgact agcaaggagt
    10981 gacaatatgt tagcacaaat caatcgagtt gcttttgagc ttgtagggat tcctatctac
    11041 tggtatgcat tgatcatcgt atcaggtatc attatcgcca tgtggcttag cagcagagag
    11101 gctgttcggg taggattaaa agcagatgat gtgacagatt ttatgttgtg gggattgcct
    11161 atctccatta ttggtgctcg cttatattat gttttatttg atcttcccca gtatatagct
    11221 gatccaatcc aaatattcaa tatccgttca ggaggcttag ctatttatgg cggactgatt
    11281 gcaggcgcga tcacgctgta tttttttact aaatatcatt ttatttcttt gtggactttt
    11341 ttagatatcg ctgcaccaag tgtattatta gcacaggcaa tcggtcgttg gggaaatttc
    11401 atgaaccacg aagcatatgg accagacacg acgcgaagct tcttagaaag tctgcattta
    11461 ccgcaattca tcattgataa tatgtatatc gaagggatct atcgtcaacc aacatttctt
    11521 tatgaatcac tttggagttt gattggattt attttgcttt tgtttttacg aaagaaacca
    11581 caccttttga aaaaaggcga agtcctttta tgttatctaa tgtggtatag tttcgggcgt
    11641 ttctttattg aaggattacg tacagacagt ctttactttt taggtgtgat ccgtatttct
    11701 caattattat ctgctgtgtt atttgtcggt acattgattc tatttatttg gcgcagaaaa
    11761 aaaggaaatt taccggatta tgaccgctca ttaggaaaaa atcaacaaat tatctgattt
    11821 ttagagcgtt aaaaatgaaa aaaatttcag gagtggtaaa atgaattata gagtagcggt
    11881 tttaggacca ggctcttggg gtactgcttt ggcacaagtc ctcgccgaaa acggacatga
    11941 cgttcggatt tggggacatc gtgcttcaca agtagaagaa atcaacaccc aacacacgaa
    12001 tcaacgttat ttaccagatc attatctacc cgattccatc caagcattca ccaatatgga
    12061 agatgcagta aaagacgcag atgcggtgct attcgtcatt ccaacgaaag caatacgttc
    12121 tgttgcaaaa gaactagtgc ctaaattgaa tacaaagcca gtgatcatcc atgcaagtaa
    12181 aggattagaa cagggaacac ataaaaggat ctctgacatc ttgttagagg aaatcccagc
    12241 agaaaaaaga aaagctgttg ttgttctttc tggaccaagc catgcagaag aagtagcgaa
    12301 acatgacatc actacgatca cagctgcaag tacagatgca aaagcagcaa aatatgtaca
    12361 agagctgttc atgaataatt attttcggat ctataccaat cctgatgtga tcggtgtaga
    12421 gatgggggca gctttgaaaa atattattgc tattggtgct ggcgctattc acggcttagg
    12481 gtttggcgat aatgcaaagg cagcaatcat gacgcgcgga ttagctgaga tcagccgttt
    12541 aggtgtagca atgggtgcca acccgctgac ctttatcgga ttaagcggag taggcgatct
    12601 agtcgttact tgcacaagtg ttcattctag gaactggcgc gcaggcaact tgttaggaca
    12661 agggcagccc ctagaagagg tgctagccaa tatgggaatg gtcgttgaag gtgtgaatac
    12721 tactcaagca gcgatggaac tagcccaaat aatggaaatc gaaatgccca tcactcaaac
    12781 aatctatgaa gtgctgtatg aacaaaaaga aatcaaaaca gccgcaaaag aaatcatgtt
    12841 acgtgatggg aaaaaagaaa atgaattctc attaacaatt ttttgaagga gcgaatgtat
    12901 atgaaagtca aaaaagcagt tattccagcg gcaggattag gaacaagatt tttaccagca
    12961 acaaaagcta tggcaaagga aatgttgcca attgttgata aaccaacaat ccagttcatc
    13021 gttgaagaag cgttgaaatc aggaatcgaa gacatcttga tcgttacagg aaaagctaaa
    13081 cgaccaatcg aagatcactt tgattcaaat attgaattag aaacaaacct aagtgaaaaa
    13141 ggaaagaccg aattattgaa attagtagaa gaaacgacag atgtcaactt gcatttcatc
    13201 cgtcaatctc atccaaaggg tttaggacat gccgttcttc aagcaagagc ctttattgga
    13261 aatgaaccgt ttgtcgttat gctaggcgat gatatcatgg aagatgaagt accattgaca
    13321 cagcaattaa tcgatgatta cgaacgtact catgcttcta cgatcgccgt tatgcaagtt
    13381 cctcatgatg aaacttcaaa atacggaatt atcaatccag gagaagtatt ggaagatggg
    13441 ttatacaatg tcaaaaactt tgttgaaaaa ccagatccaa gcaaagcacc aagtgattta
    13501 gctattattg gacgttactt attgactcca gaaatttttg atgtattgtc cgagcaacaa
    13561 ccaggagccg gaaatgaaat ccaactaact gacgcaatcg atacattgaa taaaacacaa
    13621 cgcgtatttg cccgtaaatt cacaggtaaa cgttacgatg tcggtgacaa atttggtttt
    13681 atgaaaacaa gtattgaata tggtctaaca catccagaag taggtgcacc tttacgtgag
    13741 tatatcctgt ctttaggtaa aaaattagaa gcagaaggaa gcaagatcga tcataatcca
    13801 aaaggaataa cagaaatcaa agaagaaaag aaaaaataac ccttttttct aacgagcttc
    13861 agctgctaat tgaataaaaa gtagttgaag ctcgttttta tttgtttaaa aaacacgaaa
    13921 atgctatcct tatagaaaag agagacaaga agggagtttg atcatgtcag gaggagaaat
    13981 tgcaggattg atcgcagcaa tcgcctttgc gatacttgtc gtttttatcg ttcgtgtgtt
    14041 gttgcaagtt tctaaaactt tagaaaaagt agatcagact gtagcagaag caaatacgac
    14101 gatcgaaatc gtcacaaaag acgtagatct tttgtctaga caagtagaag gcttgctggt
    14161 caaaagtaat gaactgctct tggatatcaa tggaaaagtc gcaacgatcg atccattgtt
    14221 cacagcagtt gctgatttaa gtgaaagtgt atcagaactt aatcattcta gtaaaaacat
    14281 cgcgacgaaa gtcggcggaa tcggtaaaac gacagctaaa gcgacggttg ccggaaaagt
    14341 cggtgggaca gcgatgaaat tttttaaaaa caaaaaacat actgaaacga caggaggaaa
    14401 ataaaaatgg ctaaaaaagg tggattttta ttaggtgcag tagtcggagg aacagccgca
    14461 gcaatcgccg cgttattgtt agcaccaaaa tctggtaaag aacttcggga tgaattagct
    14521 gcccaagcgg atgatttgat ggatatggct tcagattaca cagatatggc gaaagaaaaa
    14581 tcgactgagt taacaggtat tgcaaaagaa aaagcgcaag atctttctca acaagcaggt
    14641 gatctagcag gtagtgtcaa acaaaaagta agtgaatcag cagatgaagc aggaagtgaa
    14701 tctgacgata tcttgttaaa tcttaaagaa caatcagctg acttatctaa tcgtttcaaa
    14761 aaaacggcag aagatatgaa aaaacctgca gatgaattag gaagtattgc taaagatact
    14821 tcagaagata tcatgattga tgtaaaagac gctgctggta aagcaggaga tactgtcaat
    14881 gaaggaatgt cagaagcaaa accagttgca gatgacacaa aagaaatgat gaaaatggca
    14941 aaagaagatg cgaaagaagc taaaaaagaa ataaaagata caatgacaga ttcatctaca
    15001 aatcactaaa atcactaaaa tcacaaattt atagaataaa aaggtgggag cggactagct
    15061 ctcacctttt ttactcttat ttttctaaaa ctagtaattc ttttggtgta gtcgtgaatg
    15121 gcacacagcc atcgcttgtc acatatacac agtcttcgat acgtacccct actttttcag
    15181 gaatatagat tcctggttca agagagaagc acatcccttc ttcgattact aagtcatttc
    15241 cttctacaag ggaagggaat tcgtggacgg ttgttccgat cccgtgacct aaacggtgat
    15301 tgaaatattc gccgtagcca tagctgttga tcacgtttcg tgcgatttga tcaagttcac
    15361 ctgctgtcac acctggacgg acggcttctt gtgcggcaag ctgtgcttca agagtgatgt
    15421 tgtatatttt ttcttggaag tcggttggtt tttgataagc aattgtacga gtagcatcac
    15481 tgcaatagcc attccaaaca acgccaagat cgaataacac aagttcattc ggtgaaatct
    15541 tcgtgtttcc tggtgtacca tgagggcttg cagcatttgc tcctgcaagg actaatgtgt
    15601 cgaaagacat ttgtgaaaca ccttgttttt tcaattggta ttcgatttct gcaatgatcg
    15661 cttgttcagt cacgccttct ttcactgctt taaagccaat ctcaaatgca acatcggccc
    15721 atttaccagc ttcgatcagt ttgtcttttt cctctagagt tttgatcagt tgtagacgtt
    15781 ggatgatgga agctagatca gtgtaagaat ccgttcccgt caagatatca gagatagcat
    15841 cgaagcgctc gacagtcaag gcgttctttt caatccctac attgccaggc tgaccatagc
    15901 gagcacgaag ttcttttccg atgatctccc aagggttttc gctgtctaaa tagccacgga
    15961 catcatacgc ccaactactg ttttgagcat cttcaacttc caaagcagga gtgaaaagga
    16021 aaggatcttg ttcaagaggg ataaaaagag ctagtacacg ctcatgagga tcactttcgt
    16081 atccggaaaa gtaagcgatg tggccagggt cgctgatata agccaattcg acttggtgtt
    16141 gtgccatcca ttgacgtagt tcattgattt ttacgttatt catttggcaa cctactttct
    16201 aaattaattt tgtattcaat aattgtacca ctgatttatt aaaagaccaa cgttctaaaa
    16261 actgaaaaaa gtaaaaattt tcatgaaaac agagataaaa cggttgcaat tcttgagatt
    16321 atttgatatt ctattttaga aatgtaagac gtattttcag aaaacatata aaatatgaat
    16381 aggagaacac aaatggaaaa gcaaacaatc actatctatg atgtagcgag agaggcaaat
    16441 gtttcgatgg ctactgtttc acgtgtcgta aatggcaatc caaacgtcaa accagcaaca
    16501 agaaaaaaag tcttggaagt tatcgatcgc ttggattatc gtccaaacgc agttgcgcgt
    16561 ggcttggcaa gtaagaaaac aacaacagtt ggggtaatca tccctgatgt cagcaatatg
    16621 ttctttgctt ctttagcacg tggtatcgat gatgtggcaa caatgtacaa atacaatatt
    16681 atcttagcaa attcagatgg taacgaccaa aaagaagtca atgtattgaa taacttactg
    16741 gctaagcaag tggacggcgt gatcttcatg ggtcaccata tcacagacga aatccgtgga
    16801 gaattttcac gttcaaaaac tcctgttgta ttagctggct ctattgatcc agatgaacaa
    16861 gtaggtagcg taaacattga ttatgtatca gcaacaaaag atgcagtaaa caaattggca
    16921 aaaagcggca ataaaaaaat cgcttttgtc agtggtgcgt tgatcgatcc tatcaatgga
    16981 caaaatcgtc tgaaaggcta taaagaagca ttaagcgaaa ataacctacc atatagcgaa
    17041 ggattgatct ttgaagcaca atacaacttc aaagatggtc tatctctagt cgatcggatc
    17101 cataatagcg gtgcaacagc tgtctatgtt tcagatgatg aattagccat cggtattttg
    17161 gatggtttat tagaccgtgg cgtgaaagta ccggaagaat tcgaaatcat cacaagtaac
    17221 aattcactac tgacagaagt agctcgtcct cgtttaacaa gtatcacaca accattatac
    17281 gatttagggg cggtatctat gcgcctattg acgaaaatga tgaataaaga agaagtcgaa
    17341 gaaaaaacga ttatcttgcc ttatggaatc gaagaaaaag gatctacaaa ataataaaag
    17401 gacaaccatt cagaggttgt gagcctgccg tttaactctg cgttacaagg agcaatttca
    17461 aaaaacagca tttcatgttt gatgaatatt gtgactcgtg attcggagtt ggcaggagaa
    17521 caccgtttat tcgggaaaag gggttatgac aaaattttgt ttaatccttt ttttttgtct
    17581 taaaaaagag agaaagtggt tgaaattaca tttatcgtat ataaaacaat ccgtgtatag
    17641 atgctgcatt atgttcacca ccatcaaaaa aataagggac tgtgtattct attaaatcaa
    17701 tcatctttct catctgattc tttggttgct tgattcgctc ttgcataagc tgaagcggtg
    17761 ttccagtaat ctgtatagtt ttcatcggta ccgccaacgc caaatttaaa ggcgcttttc
    17821 ttcggtccat ttttcgccca tagcgattca actgtttttc ctggtgtttg gatggatttt
    17881 ttattgacag taacttttcc aggtgtttga ccagtaaatc cagatacttt tttcttcatc
    17941 acatcagatg ataattcgaa tttctcatca gtgccaaaaa tgtcaggttt tgcttggtaa
    18001 gcttgattga tcagattggc aagataatta gccgaacgta tccttgcttt gctgtccatt
    18061 ccagtattgt catcgtgccc tgcccagcta ctgatggtaa tagacggagt agagacaatt
    18121 agccatgaat ctcggtattc attggtagaa ccagtttttc cgacccaatc tgcttttcct
    18181 agatttccgt ttaagcttga gatggcatct ttgaatggtg tagtgatttt tgcattgatc
    18241 acactgcgca tcatgtcatt catgatcgat gccgttgctt ccgaatagat ccggacagag
    18301 ttgctttcgt gtttataaat tacatttcct gcgttatctg tgatggaatc gatgatatag
    18361 ccttcttcat agacgccgcc gtttgctaaa gcctgaaatc cattggtctg ttgaagcgtc
    18421 gtgacatcta ctgtacctaa aggagcagat tcaacgcccc aattgtcatt tgccgggtag
    18481 ttcatctttg ccaagtagtg ctcgtaagca tattgtttcg atccaccgga attcaacaca
    18541 tcttgatata agtgataagc cgggatattg ttggaccatt ctagtgactc acggacggtt
    18601 tggaaggtgt tcgatccttc gttagtagca ttgacgattt tctcgccagc attttctcct
    18661 tcttgccaag ttgttgcata atctgataca cgggattctg atccgataag ccccatgtcg
    18721 attgcaggtc cataaacaag aactggtttg attgatgagc cagcttgacg ttctgtatcg
    18781 aaggcatggt tattttgatt ctgactgtaa ttccgtccgc caacgaatcc gtagatgcgg
    18841 ccagttctgt tatccatcaa tacatttccg gtttctactt gactgccagc accatcatct
    18901 aacatgtatc cataattttc tacgccattt tgcaatgcag catagacatc tttatcgatt
    18961 gtcgaatgga ttgtatatcc tttattttgg atcgtttgct gtgccttttg ataataagcg
    19021 tcactgaccg atgaatcaga catatcagcg ttgtcttttt cagccaattg ttgtgcaatg
    19081 attcttgttg cttcattcat cacagtataa tacaagaatt ctctgtcagt ctgttctgcg
    19141 atttgttgtg gtagaaagtc ttttgtaagg tcataggctt ttgcttcttc gtattgttct
    19201 ttcgtaattt ggccttcacg atacatactg aataaaacaa aatcttttct ggcaaggccg
    19261 gcagaaagat cttcttttag cgcacctgtg ttcgtgtatg gactatacgt gatcgggctt
    19321 tggggtaatc cagcgatata agcagcttgc ggcagcgtca catcttttgc atctacacca
    19381 aagattcctc ttgcagcttc ctggactcca gcaatgtttt gtcccttatt attacgacca
    19441 aatggtgaga cattcaaata cgtagcaatg atctcatctt ttgaaaagta tttttctact
    19501 tgtgcagaaa gaatgatctc gtttgcttta cgtttgaatg ttgtttcatc tgtaaggatc
    19561 tgctgtttga ctaattgctg tgtcaaagtt gatccaccag aagagccaat accagtagct
    19621 tcagatacta atgctcgtaa aacagcttta ggaacgtaac cttggtgttt atcgaagtat
    19681 tcatcctctg tgctgataat tgcagttttc aataaaggtg aaattttatc agatgaaata
    19741 gtcgtgcgca tcagatctgt ctggattttt gatatctctg tattatctgc ataaacaatc
    19801 tttgacgttt ctgtaatgtt tcctagttct gtctgcagtg cttttttagt cggcaattgt
    19861 gtgtcttcga cgagataggc aaaatagcca gcgcctattc caatacctag agctgcgaac
    19921 atcaaaagaa gaacagtaat aaaaacagtc aatgattgaa aaactctaaa aataatctta
    19981 ggaacaaacc atttttcttt ttttcttcta ttctgttttt ttcgagtatt tttgcgtggc
    20041 aaaagggtca cctcaactaa tggttttatt tcgctaatta tagcaaataa tagaacaaaa
    20101 aaacagacaa ggctgttttt ggcaggaatt tcttttttaa aagagttaaa acgtgagaaa
    20161 atgatctttt ttataaaaga gttcttaagc ttttctttag agaaaatttc agttactatg
    20221 gagaacaggg caaagaaaaa tcaatcattt tctttgtata tcatcttacc aatatatagg
    20281 aattgttagt aacaattgcc tttcaaggag gaatccatcc atgttattag gctcacacgt
    20341 cagcatgagc ggaaagaaaa tgctgttagg agcagcagaa gaagcagcca gttatggatc
    20401 ttcgactttt atgatctata caggcgcccc tcaaaacact agaagaaaag ccatcgaaga
    20461 gatgaatatt gatgccggaa aagcgtacat ggcagaaaac ggtctaacaa atatcgtcgt
    20521 tcatgcccct tatatcatta atttggggaa tacgatcaaa ccagaaatgt ttccttttgc
    20581 cgtccaattt ctgagagaag agattgttcg tgcggaagct ttaggcgcta ctcaaattac
    20641 acttcaccca ggtgctcatg taggggcagg aacagaagcc ggaattgcta gaattattga
    20701 aggcttgaat gaagttttga caaaagaaca gacggcacaa atcgctttgg agacaatggc
    20761 aggtaaaggt acggaaatcg gtcgaacatt tgaggaatta gcgcaaatca tcgatggagt
    20821 aaaattgaat gaaaaattat ccgttacttt tgacacctgt catacgaatg atgccggtta
    20881 taatattaaa gaagactttt caggtgtaat ggaagagttt gataagatca tcgggttaga
    20941 tcggcttaaa gtactccata tcaatgattc taaaaatccg caaggttcgc ataaagaccg
    21001 gcacgccaat attggttttg gaacaatcgg attcgacacg ctgaataaga tcgtccatga
    21061 tccaatgttt gacaatattt cgaaaatctt ggaaactccc tatgtcggca aagataaaaa
    21121 gaaagcttac gcaccatatg gaaaagaaat cgccatgttc aaagcacaaa tattcgatcc
    21181 agaagtgttt cctgagttat tagcagaaga agtgatactc taagattttc tatctgacag
    21241 cagcaaaaaa ggaccaggca cattgccttg tccttttttg tttaagaaac aaaggagtgc
    21301 tttttgcagt ctttttcatc atgcatcttc tcccccctct ttttaaattt agacaaacag
    21361 ggtatactgg tagaaaagta aaaaaacgaa aagtttcaca agcaagtggc aaaacgtgat
    21421 tggaaggacg aaaagatgct gaacacttta aaacccttga tcaaccaact aaaaaagaaa
    21481 catacatact ttattcttgg catagcccta ttatttacaa ttattcgttt gtatttatat
    21541 agtaaagcaa tctatggaac agacatgtcg gcaacctatg atgaccagct gctgattcgc
    21601 tatgctcaaa atattttgga cggtaaatgg ctaggggagt atacgactac aacactttcc
    21661 aaaggaatct cctacagctt atttttagta cttgcacata aactgcatct ttcttatgca
    21721 gttttgttcg gcatgttcag tatttttgcc agtatattgg ttgcactcgc gtttaaaccg
    21781 atcgtgaaga acaagtatct tcttttaagt ttctattttt attttttgtt ttctccgatt
    21841 agttttacac atgaatattc cactcgaacg tatcgtaatg ccctcgtgat tccggctgtt
    21901 gagatcatta tcgcttgttt actagctatt tattaccgta aatatagttc cagcaaaaaa
    21961 atgctgccgt ggtttttagg gctagctgtt tcttttcctt tcttttggtt cattcgcgaa
    22021 gattccatct ggcttcttcc ttttactgta gtcgcgttag gcatcagttt cgtccagatc
    22081 ttgctaggac aagacttttc tttcaaagaa gcaaagaagt taaaagaacg accatttctc
    22141 ctttcgaaac aagaatggct gaaactttca ttgtttttac tccctctagt cggtttctcc
    22201 atcgtctatc agtggatcga atataaaaac gagacaacat atagtattgc cgccatcaat
    22261 gaccgttcga gtggcgaatt tggacgtttg atgaaacagc tgatccgaat agatgacgaa
    22321 acgaacttaa ataaagaaaa cagtgagata tgggtctcgc atgctgcatt aaataaagca
    22381 ttagacgcat ctcctacatt tcatgctgtt tctactcgta ttgacgaatt gtatacgact
    22441 catgcgtgga cccaaggtgg aaaagtaaaa gaattaccag gagatatcat tttttgggca
    22501 ttgcgagatg tggtaagcga atctggttat taccaaaaca atgcaaaaga aaccaatgat
    22561 ttttggaaaa aagtcaatca agaattagaa gaagcctatc aaaagggaac tatcaaaaag
    22621 aaaaaagaat tctatctgat gggatcaggg gatggaaaga cgatgaatga tctgccaatc
    22681 gtatttgctt ttctgaaatc aggatatgcc tatgcatggc gatacaaaga tttccatcaa
    22741 ggggggaatt atagtttcgg tacgcaagaa caagtcgaac aggcccaaga attattgaat
    22801 cttccactaa tggataattg gatcgatagc agtgatccga gaacacctga tttccagttg
    22861 acaaaaacag caaaagtcgc gaatgtcatg ataaaaattt accaagttct ggccattccc
    22921 gtgctgattg cttcaatagc agggatatta cttatgatac taggaaccgc atttgcaaaa
    22981 gctgatcgtg cctattatag ttcctctttg attgtagtca ccggattaat actgacacaa
    23041 ttggtttttc ttttcggcgt ctcgtggttt tgttcttatg cgccagaaaa aaaggattat
    23101 tttttaagtg tctacactgg cgcaggcgtg ccattaacgc aattcatcgt agtaacaagc
    23161 atgctagttt tcagtaaatt gaatctgaaa aaagctttat cttccaaata aaaacatact
    23221 aagattagag gagaacatca ttgacgaatg atgttctcct ctattttcta tttttagatt
    23281 atagtgaaga gtgaaagaaa gttcgaacaa agccttgagg actatgagtc cctatcaaaa
    23341 accgaacttc tttactgtca ttttagaatc aggtttacgt atgttggggc catgagcccg
    23401 tgtataggaa attggaatcg aaatgaagca gtaaagttct ttcaaaactt aaaaaggagg
    23461 aaaacaaatg ccttacattc ttgcattaga tgtttcaatg ggacacagct acagtgtcct
    23521 atacaaagac gacacctgct tatttgaaga cgagatcgaa cacactcaaa gaggatttca
    23581 tgcattactc gaagagatcc agcacttgcc tgaacgtcca ttgattgttt ttgaagcaac
    23641 ggggatttat tccagaccag tcgaaaagtt ctttcaagat catcactttt cttactgttt
    23701 gttaaacccg ttagaagcga aaaaacagat ggaagaaaac accttatgca gttggaaaac
    23761 agataaatcg gatgcccatc ggctcgcaca gactcatctt aaaaatcaac gtcagccaaa
    23821 agaagtacaa agcaattttt acttagagat gcgcgattta gcccgttttt atcaagaaat
    23881 tgagaagaaa atcactcgtc tgcgcatgga tttacacaac tgccttcagt tgacttttcc
    23941 agagcttgaa cagttctttt taaacaggct gaccccttac gcattgatat tgatccgtct
    24001 ttttccccat ccagattttg tattggcatc tactcggaca aaaataaaaa ataaactgat
    24061 caacgaaaca cggaaaaaaa tctccgcgaa tcgtgccgag caaaaggcag atcaaatcat
    24121 ccactatgca cagtgtgcct atccagctgt tgaaaaagac agtatccact gtcaaaaaac
    24181 gatctattac gctgaacttc tccaagattt actcgaacaa aaagaagcac tggcgacaca
    24241 aatgatcaaa aaagccgaag gctctccttg ctttttgttg taccaaacgt tccctgggat
    24301 tggcgcactc acagccgctt tattgctcgg tgaattagga gatatcacac gatttaaaac
    24361 ccataaacaa ttgaatgcct ttgtcggaat cgacatccga cggtatcatt caggtaagta
    24421 tactggccaa gaccgcatca ataagcgtgg taatcccaaa gcacggaaaa ttattttctt
    24481 caccattcga aatatgattc gccagcaacg cgcagctcct aatcatattg tcgattatta
    24541 ctacaaatta aaaaagcaac ctatccctaa gaaagataag gttgccacag ttgcttgtat
    24601 gaacaagctt ctgaaatgta tgcacgctat ggttagggca catacagagt acgactatgc
    24661 gtacgcggtc tcagtggacc attaactaaa ccacttacag tataccactt ctttgcccag
    24721 atcgaaaatt ttttacgaac ggggctttat ttggtgtgcc ttgattttgt cgctttcgtt
    24781 tatgagctat ttttcttaca ccttttactt gactaatcgt aggaaaggga actgtgacaa
    24841 aagtcttgtc gcagttcctt tttccgaata aacggtgctc aaaagcttgc atctgcggta
    24901 ataagtccaa ccaagttaaa aacataaaaa gctgttttta cttggctgtt cttattactt
    24961 gatgcaaggc agctttttca caacctctga tacacggtgt tccagaagta acttcttcgg
    25021 aaataagccg aaatttcaca aaaatttgaa aagcaatttt cttaaattcc ttcttatttc
    25081 ttgaagctgc ccacttctga cacaacctcc tttgtgtaaa agaatcagtt tctactcgat
    25141 ttcttctatc gtttcctgca aacgctgttc atattttttg tttcctttat ataaatcacc
    25201 aatcgtccat gccaacagga gaaggacaag aacaatgatt acataagcga tttgatcagc
    25261 caatgctagt tgagctgagg tggcttgctc gccgaagaaa ctttcgattt gacctggtaa
    25321 tcctcgtaaa ttcaagtaag tcagactaat cacagagaac cagccaagta gttttacaac
    25381 aaatgaattt ttgaatcggc tccccatctc cgcttggcta ttggtcatca tcagtaaagg
    25441 aatcatcgag aatggcaatg caaaagccag aaaaacttgg gattcattca tcaagttatt
    25501 caaagcggta tgttcttcga tcgttccttt tgtacttgta aacaaaacac aaatcaatac
    25561 aggaatcaca gaaagcaatc gtgtgactag tcttcttagc caaattggga tacgcatatg
    25621 gatgaagcct tccatgatga cttgaccggt caacgtacca gtaatagtag agttctggcc
    25681 agaagcaaga agtgctacag caaataaagt agacaagata ccagattttg ctacagcaat
    25741 caagatacca ttgctcatag ctgatggatc agataaagct tcatacagac caaaaaagga
    25801 aggatcttgg acagaaccgg ttttgaatac agccacaccc atgatcaata gcagtgcatt
    25861 gactagaaaa gccattgaca actggatatt agaatcccaa gtagaaaagc gtaccgcttg
    25921 tgctacatct tcttcgtctt ggtgatcgat cttccgagtt tgagaaactg ctgaatgaag
    25981 atataagtta tgcggcatga ctgttgcacc aataatccct aaagctccag tcagcggagt
    26041 ttcgccgccg atgaccggtg agtctgagaa tgtgtgactg ttagggataa atccggcaaa
    26101 caaactgtgc caatcagggt tagacaagcc aacttgatag acgaaaacga ataaaatgac
    26161 gaaaatcaag cagacaacca aagcttcgat tttacgaaac ccgacttttg tcagcaataa
    26221 aaggacgaaa acatctaaca cagtgataaa tacggcaatt accaatggaa tatcaaaaag
    26281 caggtagagg gcgattgctg caccaatgac ttctgcgatg tctgttgcca taatggctaa
    26341 ttcagtcaag atccataaaa tgacacccaa tgtcttactt gtccgtgctc ggattgcttg
    26401 ggccaaatcc atttgggtca cgattcctaa ttttgctgcc atatattgta aaagcatagc
    26461 aatcagacta gacatcaaaa taactgacat caataaatat tggaaatttt gtccaccagt
    26521 gatcgaagtg gaccagttac ctggatccat ataaccaact gctactaatg cacctggtcc
    26581 agagtaagca agaagtgttt taaaaaaact ttttcccttg ggtacatcca ctgttccatt
    26641 tacttcagct aaagataaac catttgtatg ttcaataagt cgatgcttat gaaattgttt
    26701 gttggattga ttattcaaga aaaaaccacc tttcctattt atctacgatt acattctaca
    26761 cctcagaaag aaaaaattaa agaaaaagat ttggtatgcc taattttttt tcgtggcgtg
    26821 tcttgctggt taatgaaaga aattttgttc ttctctaaaa aaaccactat tttttgcatg
    26881 aaactaaaga atacgccttt cttttttcaa taaactttag taaaataaat cattgagagc
    26941 aattcggaaa tagaaaggag agatttatat ggtatcaaca ggtattgtcg tacttattgc
    27001 tattattgct ttgctaatcg gcgcagtagg tggctttttc ctagcgcgta aatatatgaa
    27061 agactattta gaaaaaaatc ctcccgttaa tgaggacatg ttgcgatcaa tgatgatgtc
    27121 aatggggcaa aaaccatctg agaagaaaat tcgccagatg atgcagcaaa tgaagaacca
    27181 agggaaaaaa taggttctga cccaaagact ttttgcgggc tgattttttc agtccgtttt
    27241 ttatttcgta atttgttaga ctattcagac tattcagcaa aaaagacaag cacagacaat
    27301 cctatgctct tgataaagac aaaggaggag taaaatgtcg atttttagaa aattaggttg
    27361 gttttttcga caggaaaaga aacattattt aattgggatt ttctccctat tcgcagtggc
    27421 cttggtacag ttagctcctc caaaagtgat tgggatcatc attgatgaga tcgccgataa
    27481 aaatttatca ataaagatca tttcctggtg gatcttatta ttgatccttg cagctctctt
    27541 acaatatctt tttcgttata tctggcggac gaatatttgg ggaagtgcag ctcggttgga
    27601 gaaagaactt cggcagcagc tattcaaaca ttttacagaa atggacaatc tcttttacca
    27661 aaaatatcgg actggagatt tgatggctca tgcaacgaat gatttgaatg caatccaaaa
    27721 tgtagcagga gctggtatct tgacctttgc agattcagtg atcacgggcg gaacaacgat
    27781 cattgcgatg attttgttta ttgattggcg cttaacactg attgcgctga ttcctctgcc
    27841 tttactagcc gttacttcac gagtgctggg gtcaaaactc cacgatgctt ttcgtgattc
    27901 gcaagaagct ttttctagta tcaatgataa gacacaagaa agtattactg ggatcaaagt
    27961 cattaaaaca tttggacaag aaaaagaaga tctcgcggat tttgaagaaa agattgaaga
    28021 tgcaattatt aaaaacaaac gagtcaattt tttagactcc ttatttgatc cattcattac
    28081 cttgattatc ggtctgtctt atgttgcaac aattatttta ggtggaaagt atgtcttgga
    28141 aggcacgatc tcaatcgggc agatggtctc atttattagc tatatcggta tgcttgtttg
    28201 gccgatgttt gcgattggtc gattgttcaa cgtattggaa agaggaaatg cttcttatga
    28261 tcgcgtcaat gaacttttgc atgaaaagac gcatatcgtg gaacaactgg gtgcagtgca
    28321 gactccggca aaaggcgagc tgaatgtagc tatttcctct tttaactatc cgaaagaaga
    28381 atcaccagca ttagaacaaa tcaaatttgc gatccaagaa ggagaaacat taggtattgt
    28441 tgggaaaact ggtgcgggaa aaacgacaat catcaaatta ctgatgcgtg aatacgatga
    28501 ttatcaagga aaaattcaat ttggcggatg ttcgatcaaa gaatattcat tgaatgcttt
    28561 attaggatca atcggctatg tgcctcaaga tcacttcttg ttttcgatga ccattcgaga
    28621 taatatccgt ttttctaatc caaaactttc agaagaagaa gtagagcatg cggcttcttt
    28681 agcatttatc aacaatgaaa tcaaggaatt accaaaaggt tatgatacct tagttggaga
    28741 acgtggagtc tctttatctg gcggacagaa acaaaggatt tcgattgctc gagcattgat
    28801 caaaaatcct gaattgctga tcttagatga tgcactttct gcagtagatg ccaaaacaga
    28861 agaagctatc ttgcataatt taaaagaaat aagaaaagag aaaacaacca tcattgcagc
    28921 tcaccgttta agcagcgtga tgcatgcaaa agaaatcatt gttgtcgatc aagggaaaat
    28981 cattgaacga ggaacccatc atgagctgct tgcattaggc ggctggtatg aacgaatgtg
    29041 ggagaaacag aaattagaag cgaagataga aggaggcgag gcctgatgga tgaaaattat
    29101 caatcggaat ggacaaaatc gattccactt aaagaacaga agaagatcat caaacgactg
    29161 atgaagttcg ccaaaccttt tagaagaacg ttcatcgttg ctatcttatt tgcgtttgcc
    29221 ctgtctgtga tcaatgtcct attgccaagg atcatccaaa cttttatgga tgatcatctg
    29281 gctaaacagt cggctacgac acaagttatt ttgttttttg ctggtatgta cttgtttgga
    29341 gtcatagtca aaagtatcat ttggtttttc caatggtatt tgtattccat ggcttcattg
    29401 aagacctatc agtatgttcg ggtaagatta tttgaaaagc tgcatacact aggtatgcgt
    29461 tattttgatc agacaccggc aggatcgact gtttctcgag tgacaaatga cacagaaacg
    29521 ctatttgaat tttggtatgt ctttttgatg gtattgactg ggatctttgc agtcgtttct
    29581 tccttcattg ccatgtttca aatcaatgga gaaattgcct tgtggacttt ggtgttcttg
    29641 ccgattttag gaatcgtcat ctggtattac cagaaattca gttctcgtat ttaccggaat
    29701 atgcgggaaa aattaagtca gctgaacacg aaattgaatg aatcgatttc cggcatgcgt
    29761 attatccagc agtttcgcca agaagctcgt ttagctaaag aatttgaaca agtgaacaac
    29821 gagtatttag acacgcgcta tgcgatgatc aaaacaaatt cgattctgtt aagtccgatc
    29881 atcaattttc tatatgcatt ggcgattgcg ttggcgttga ctttgtttgg gatcgatgcg
    29941 ctgcattctc ccgtagaagt tggactgatc tatgcattta cgacctatgt ccagggattt
    30001 tttaatccaa tgtctcaaat gatggacttt ttgagcacct ttacagatgg aatggttgcc
    30061 ggtagtcgta ttttgaaaat catggataca gaagaatatg caccgcaaca aaatcctgga
    30121 gcagatcaag cgattacttc aggtaagatc gagtttcgca atgtaagctt ttcttatgat
    30181 ggagagcaca atgtattgaa caatatctct tttgtcgcta atccgggaga gaccgttgca
    30241 ttagtggggc acaccggaag cggaaaaagc tcgatcatca acgtattgat gcggttttac
    30301 gaattttatg aaggccagat cttgattgat gatcatgaca ttcgagaata tccaatggaa
    30361 gagttacgta agaaaatggg attggtcttg caagatgcct tcttgtttta tggagatatc
    30421 gcaggaaata tccggatgat gaatccaact atcacagacg aacagattcg ccatgcagct
    30481 gagtttgttc aagcaaacaa gttcatcgat gaattaccaa aaggatatca ctcaaaagtg
    30541 atcgaacacg gtgccagcta ttctagcgga caacgacagc tgatcacttt tgcgcgaaca
    30601 atcgtgacag atcctaaaat actagtattg gatgaagcga cagcaaatat cgatactgaa
    30661 acagaaggat tgatccaaga aggattgaca aaaatgcgac aaggacggac aacgattgcg
    30721 attgcccatc ggctgtccac gattcgggat gcagaattga ttttagtttt agacaaaggt
    30781 catatagtag aacgtgggaa tcatgaaaca cttctagcgc aaaatggttt gtatgcagat
    30841 atgtacaaac tgcaaagcag tggtgtgtaa aaagtggttg tgacagggct ccgttcagct
    30901 ttaggtgata agaaaaaaat tgtaaaaaag gctttccaga tttttgcata aatttggctt
    30961 atcaccgata agctggtact ggaacaccgt atattagaag ttgggacagt agcatagtag
    31021 cattttgacc taagcgctag agtgaaaata aaacagagct tcccagattc ttcatctgtg
    31081 agcgttagtg tcgtaattac cagatttaaa aaaaatcacg acagctagtc agaagtgccc
    31141 aatagccgct aagaaagcgt tgacaaaagt gcttgctttc gataatataa agaaaacgat
    31201 acaacttgga tagtgattgc ctgatgcaag ctaaacctga tgagtctccc atggttccat
    31261 ggacggcttt ttgggttttt ctttttgttt tggatacagc cctatatatc atggaaagga
    31321 gtaaaacatg ctgattgaga aagtactgaa taacaacgtc gtaataacca gggatgaaag
    31381 aaatcaggaa atgatcgtga tgggaaaagg tttggcattc aaaaagaaag tgggtgaccc
    31441 gatttttgac tcatgcatcg acaaaaaatt tcagttgaca aatgacggac tttcacaaca
    31501 gttccaagaa ttggcacaag cgattcctct tgagtactta gaaatttctt gtgaaatcat
    31561 tgaatataca aaagaggctc tacaagtcca gttgaatgat tcggtgtata ttgcattgac
    31621 ggatcatttg tatacggcta tcgaacgtca caaaatggga atatttgtac caaatgtatt
    31681 gctgtatgat atcaaacaac tatttcctaa agagtatgcc atcgggaaaa aaacagtgaa
    31741 gaaaatctcg gataaatacc acatcaagtt atcagaagat gaagccggat ttatcgcatt
    31801 gcatttagtc aatgcacaag gcattcaaag ccaacaagac aatacctacc tgtttactac
    31861 aacaatacag gatattctaa atatcgtccg ctattactat aagattacat ttgatgaaga
    31921 ttcagtccat ttttttcgat tcgtcacaca cctacgtttt tttctgacac gcgtcctaga
    31981 tggagaaaaa tcagaagtgg gagaagtttc tgaggaattg taccaggtta ttcaaaaaaa
    32041 atataaggat acgtttggtt gcgttgagaa aatagcagac tttttagccg agatattcaa
    32101 ctaccagatg tccacggacg aaaaaatgta tttaacgatc catattgcgc gtttagtgca
    32161 tgagaatcgt tgtaaataaa taaaaaaagg aggataaatt aacaagaacg catgaaataa
    32221 gggtagtgac attcagttgg ctaaaccttc cactaataaa ttggagggaa tagaaatggg
    32281 aaaatatcaa gaattagcga gcaaaattgt agaaaatgtc ggcgggaagg aaaatatcaa
    32341 tggtttgaca cattgtatca caaggcttcg tttcaaatta aaaaacgaag aaaaagcaaa
    32401 tgatgagatc ttgaaaaata tggacggtgt tgtgacggta atgcgagcgg gaggacagta
    32461 ccaagttgtc atcgggaatc atgtgccagt cgtgtttgaa gaggttataa aagcaggaaa
    32521 ccttactttt gatgaggctg tttcaacaga aaaaatgaga ccgtttgata tgcttattga
    32581 tggaatatcg ggctgtttcc agccattcct tgctattcta gctgctggtg ggatgattcg
    32641 tggactgaca gcattacttg tcttcttagg cgcatttgac cgcggttctg gcacatttgt
    32701 tatgtttgat aatatcggtg attctgtatt ccaatttatg cctgttatta tcggtttaac
    32761 agctgcccgt aaatttaaag taaatgaatt cgtagggatg ttgcttgggg cagcattaat
    32821 gaatcctagc ttgtcgctgg aagctttgag tggtgcagca gaagcaccac tgactaccat
    32881 attttctggc actatttttg aagcacctat ttatcaaaca gtattcggca ttccctggat
    32941 tgcgcgtaac tacgcttctt ctgtcattcc cattatcttt attgtgttgt tagctagcca
    33001 aattcagaaa ccaattaaaa agttagtacc tgagatgatc gcgaatttct tcgttccatt
    33061 cttcaccgtt cttattacaa tgcccttagg ttttttactc gttggaccag cattcacatt
    33121 cgcaactgat attttaatgg ctggatttga aacactttta gcactttcgc cagttattta
    33181 tggagcaatc gtaggcttct tctggcaaat tctagtgatg tttggtctcc attgggcaat
    33241 cgtaccaatg ggattgatgc aattttctgt gaatggttgg caaaatatca tgacacctgt
    33301 tgcagtagtg tcttttggac aaactgcagc acttactgca ctctacttta agcttagaaa
    33361 cccaaaagac aaagcaatcg caataccagc cattgtttct ggtattgtgg ggattacaga
    33421 gccagctatt tatgggttta cattgccacg taaaaaaata tttattttta cctgtgtcgg
    33481 tggtgcgtta gggggcgcgt atagtggatt aatgaattta acttcttgga atccaggtgg
    33541 attaggcatc tttacaattc ccaactatat tagaccagat ggtgatttaa cggatgtgat
    33601 taatgtattg attggaatcg ccattgcgat ggtggtatcc tttgcgctga cttttttctt
    33661 ttggaaggag gaagcaggtg aaacagatga tagacagaaa agatcaggta aagaaattgt
    33721 aaaaacaccg atacaaggac aaatcgctcc actgaatgca gcaaaggatg cagcttttgc
    33781 acaaggaaca ctaggtcgag gaatcttgat ctaccctgaa aaaggagaag tacgtgcccc
    33841 ttttgacgga acagtcatga ccctgttccc gacgaaacat gcaatcggaa tggtttctga
    33901 agcaggtctg gaattgctga ttcatgtcgg attggatact gttcagttgg aagggaaata
    33961 ttttgaaagc ttggtgcagc aaggagaaag agtgaaacaa ggcgatctgt tgctccgctt
    34021 cgacaaaaag aaaatcgaag aggaaggata cagtttagaa acacctgtga tcgtcaccaa
    34081 ttacacggat tatttggata ttttggaaaa tcaaggggag acggtcgggc cagacgatgc
    34141 acttatcacc gcactgacct gaacggttag gtgaaagaat ggactaggct taattattct
    34201 ttttagtcca cttctttatt ttcaaaattg aaaggctctt tcatgatggc tgagctgata
    34261 gggcgtttat caaatttttg tcctacagaa attagatgga ctaaattctg ttgataattt
    34321 tctgcaaaaa cacaactata taacacgtct agaagataac aaatcgatgt attgatcgta
    34381 aaattaccaa ttttcgaata cagtcgttcg cgtgtcgtaa gtcgcaaagc ggctgtactc
    34441 attgaagata gcgttgaatc accgatactg gtcaaagcta aaattggaac atgattttgt
    34501 ttcaatattt ctgcggtacg aaggatcaca ttgttttcac ccgtataaga aatcagaatc
    34561 ccgcacatat cactcgtgca attgtacgct tcatagaacg attcaccaaa agtggtgaca
    34621 atcgtgacat ccattttgat gcgcttcatt ttcagcatga aatcttgaga aatcaaagta
    34681 ttcgagttac tggcgaaaat cacgattttt tttgcttgaa gcattagttg tttggctttt
    34741 tgcagttggt catgatgaat gagagaaagt gtatcttgaa tcgtcatctg ttctaaagaa
    34801 gccattttat ttgcgatcgt cataataccg tctgtggtgg aaaaaggaag attcgcatct
    34861 acgtcttgaa aattcccttc aagataggct gtttcagcta aaaaagcatt acgaaaactg
    34921 acccagccat caaaacccat tttttttgct acacggatga gtgtggacgg gtggacataa
    34981 catcgctcag caatctcttg gatggtcatt tcgccaagtg ctggagaatc gcttaagaga
    35041 aactgaatca cttcattttc tgaaggagaa aaggagattt ctttgatttt ttcacgtaat
    35101 aacatgtaga taccttcttc ttgttttttt acgtaaatta taactttttt gaaaatgatt
    35161 tgtaaacgga tttatgttat atagcttaat aaagacattt tttgacaaga tatttacaaa
    35221 aatccagtag aaaacactaa cttttgtaaa tgttactgga aagaaacgaa tttctattgt
    35281 ttgttttgtt tacaaaaaag ataataaaag tgtaaaaagt aaaggagtga gaacatggca
    35341 aaaggaatca aaatcgctac gattggtgga ggcagcagtt acacccccga gttgatggaa
    35401 ggatttatca aaagatatga ggaattaccg atacgagaaa tctggctcgt agatgtagaa
    35461 gaaggaaaag aaaagcttga gatcgtcagt cagatgtctc agcgtatgtg ggatgcttct
    35521 ccgtatgaag tcaaaatcta tgcaactctg gatcgggaag aagcattgaa agatgcggat
    35581 tttgttacga ctcagtttcg tgtcggtcaa ttagatgcac gaatcaaaga cgagcggatc
    35641 cctagttatt atgggatgat tgggcaagaa acaaatggtg ctggcgggat attcaaagct
    35701 tttcgtaccg ttccagtggt tttggcaatc gttgaagaca tgaagcgttt atgtccaaat
    35761 gcttggctga tcaatttcgc gaacccaagc gggatggtga cagaagcagt tatcagatat
    35821 ggcaaatggg ataaagtgat aggattatgc aatgtgccag taatggcgat gatgacagaa
    35881 ccaacaatgc ttggcaagaa aaaagaagag ctgatctaca agtttgccgg tctaaatcac
    35941 ttccattggc acaaagtagc agataataaa ggaaatgacc gcacggcaga attgattgat
    36001 cgtttatacg atgaagacaa tggattgccg aaaaatatct ttgatatccc gtttttcaaa
    36061 gaacaattaa aacaaatgaa catgatccct tgtggctacc accgctatta ctaccgtgaa
    36121 gaagaaatgc tggaacatgc tttagaagaa tatcaaacga ttggtacaag agcgcagcaa
    36181 gtgaaagaaa cagaagcaga gctgtttgaa ttatacaaag atccagaatt ggatcataaa
    36241 cctgaacaat tgcaaaaacg aggtggtgct tattattcag atgctgcctg tgaaacgatt
    36301 gcttcgatct atggaaataa agaaacacag atcgtggtat ctacaaagaa tgatggagct
    36361 gttcctgatc tgccggcaga ttgtgtagta gaagtcactg cctatgttgg cgcacaaggc
    36421 gcacgaaatg tagcgtttgg ttcccttcct gctgctgaaa gaggctggtt gcaagtaatg
    36481 aagaatatgg aattgctgac gatcgaagca gctgtgactg gtgattatgg aacagcttta
    36541 caagcgttta cgatcaatcc gcttatacga tcaggaaaga cagcaaaacg gatcatgaat
    36601 gagttgttta ttgcacatca gcagtattta ccgcaattca aggaaacaat cagccggtta
    36661 aaagcagaag gaatcgaagt ccaagatgaa ctggcaagag aattatcaag caatacacat
    36721 gtaatggagg aagtatgatg ggattcagaa aagatttttt atggggcgga gctacagcag
    36781 ccaatcaatg cgagggcggt tatgatgaag gcggacgtgg gttagcgaac gtagatgttg
    36841 tgccaatcgg agaagaccgt tttcctgtca ttactggaaa aaagaaaatg tttgaatttg
    36901 atgaaaatca tttttatcca gcaaaaattg cgatcgatat gtatcatcat tataaggaag
    36961 atattgcttt atttgcagaa atggggttca agacttatcg gatgtccatt gcttggagtc
    37021 gtatttttcc aatgggcgat gaaaaagagc caaatgaatc tggtttgaaa ttttatgagg
    37081 atgtctttaa agaatgtcaa aagtacggga tcgagccgct tgtgacgatt acacattttg
    37141 attgtccgat gcatttgatc aaagaatatg gtgcgtggcg ttccagagaa ctcgttggtt
    37201 tttatgaaaa tctgtgccga gtcatcttca atcgctataa aggacttgtc aaatattggt
    37261 tgacattcaa tgagatcaat atgatccttc atgcaccatt catgggcgca gggctgtatt
    37321 ttgaagaagg agaaaatgaa gaacaaatca aatatcaagc ggcacatcat gagttggttg
    37381 caagtgccat cgcaacgaag attgctcatg aagtagatcc ggagaacaaa gtaggctgta
    37441 tgctggcagc aggagcatat tatccataca cttgcaatcc tgaagatgtt tgggcttcta
    37501 gaaaatcaga tcgagaaaat tatttcttca ttgatgtcca gtcacgagga gaatatcctg
    37561 cttatgcatt gaaagaaatg gaacgaaatg gcattcagat caaaatggaa cctggggatg
    37621 ccgcattgct gaaagaacat actgttgatt ttatctcctt ttcttattat tcttctcgag
    37681 tttcgactac tgacaacgaa ctcttggaac agactgcagg aaatatcttt gcttctgtca
    37741 aaaatccata tctggaagca agtgaatggg gttggcaaat tgatccatta ggcttgagaa
    37801 taacgatgaa tgatatttat gatcgttatc aaaaaccact atttattgta gaaaacggct
    37861 taggtgcagt cgatacgccc gatgagaatg gttatgtgga agatgactat cgaatcgact
    37921 atctggcagc acatatccag gcaatgaaag atgcagtaga attagatgga gtcgatctat
    37981 taggctatac gacttgggga tgtattgatt tagtatcagc tggtacaggt gagatgaaga
    38041 aaaggtacgg attcatttat gtcgatcgtg acaacgaagg aaatggcaca ttagcccgtt
    38101 cgaagaagaa atcattcaac tggtataaaa aagtaattga ttcaaatgga gaaaatcttt
    38161 cgaatgattg atcaatgatt tataaaagtc tattgttttt cagcggattt ttcgagtata
    38221 ataaagctat tcgagaaacg caagcagaac aatagacttt ttttcgcatt ggctgtaaag
    38281 tatgcttact tgattaagta aaggagaatc tacatatgta tgatgtaatc gttattggcg
    38341 caggtcctgc tgggatgact gctgctttat atgcttcacg ttcaaactta tccgtattga
    38401 tgatcgaaca aggcgcccct ggcggccaaa tgaacaatac ggcagaaatc gaaaactatc
    38461 ctggtttcga ttcaataatg ggtcctgatc tagcttataa gatgtatgaa ggtgtttcaa
    38521 gatttgggac agaaaatgct tatggaatcg tgcaagatat caaggatcac ggcagccaca
    38581 aagaagttat ttgtgaagat aaaacttacg aaggaaaaac agtcattatc gcaacaggct
    38641 gtatccaccg taaattaggc gtacctggcg aggaagaatt tgctggacgt ggcgtttctt
    38701 actgtgcagt ttgtgatggt gcgtttttcc gtaacaaacg tctagtagtg atcggaggag
    38761 gagactcagc agtagaagaa gccatctatt tgacacaatt tgcttctgaa gtagtcatcg
    38821 tccaccgtag agatgaatta cgtgcacaaa agatcatcca agaccgtgca tttgcgaatg
    38881 aaaaaatttc ctttatgtgg gatacagtcg ttgacgaaat cgttgggaat gaaatggtag
    38941 tgacaggtgt gaaagcacgt aatgtcaaaa ccggcaatga gtttgatttg gcagcagacg
    39001 gagtattcat ctatgttgga ctggatccat taactgcacc atttagagaa gcagggttga
    39061 cgaacgaagc tggctggatt ccaacagaca acgaaatgaa gacgaatata ccaggcgttt
    39121 ttgccatcgg agatgttcgt gaaaaagatt tgcgacaaat cactactgct gttggtgatg
    39181 gtgggatcgc tggacaacaa gtgtataaat acttggaatc tttagaagaa acaactgcat
    39241 aagtttaaaa agagtggaag aacggtttaa ttgttctttt actctttttt ttcgcgtgaa
    39301 atctactgaa tccgatttat gcgaatactt gcaaccgtgc ttgataaaag aatcctttct
    39361 tgatttaagc gttttttggt cctcatactt tcatatctaa taagtgaatg ctatacttat
    39421 taagaaccat atttcagaaa gaggtgttat tttcatgtct tgggaacaag tgtatgaaca
    39481 atgggtacat gaagaaaatt tagaagaaaa cttaaaaaaa caattaaatg atctgggtga
    39541 agatcctgaa aaacttgaag atgcttttta tgcaccgttg gaattcggta ctgcaggcat
    39601 gcggggaatc ttagggcctg ggatcaatcg aatgaacatc tatacggtaa gacaagcaac
    39661 agaaggactt gcacgtttca tggatacaca aggcgaagaa acaaaacgcc gaggtgtcgc
    39721 tatcgcttat gactcacgcc atcaatcacc agaatttgcc atggaagcag ccaaaacatt
    39781 agcacatcac aatatcccat cttatgtatt cgaaagtcta cgtccaacac cagagctttc
    39841 ttttgcagtc cgtcatttga agacgtttgc gggaatcatg gtaaccgcat cccacaatcc
    39901 agcagcttac aacggatata aagtctacgg agaagatggg ggacaaatgc ctcctgcaga
    39961 cgcagatgca ttgacgaaat atgtccgtga agtcagcaat ccgttaaaag ttgaagtatt
    40021 atctgacgaa gaagctgaac atagcggatt gatcacgatc atcggagaag aagtggatgc
    40081 cgcttatcta gaagaaatca aagcagtaac gattgatcat gaattggtgg aaacaatggg
    40141 gaaagatttg aaacttgtgt acacaccact tcacggaaca ggaaaaatgt tgggcgaacg
    40201 tgcgttgaaa caagctggat tcgaacaatt cgtactagtt ccagaacaag ctgtcgcaga
    40261 cccagatttc actacagtca aatcaccaaa tccagaagaa cactctgctt ttgagtatgc
    40321 gatacgactt ggcgagaaag aaggcgctga tttgctgatt gcaacagatc ctgatgcaga
    40381 ccgtttaggt gcagccgttc gtttgccaaa tggctcctat gaagtattga ccggaaacca
    40441 aatcggagca ctgtttgctc aatatatttt ggaagcacac caatcgcgag gcactttacc
    40501 tgaaaacgca gcggtattga aatcgatcgt ttctagcgaa ttacctacag caattgcgga
    40561 acactatggg gctaccatgt tcaatgtatt gacaggattc aaattcattg ctgaaaaaat
    40621 tcaacaattt gaagaagatc attcccatac ttttatgttc ggattcgaag aaagttacgg
    40681 ttatttagtt aaacctttcg ttcgcgacaa agacgcgatc caagcattgc tcttattagc
    40741 agaagtagca gcttactata aaaaacaagg caaaacgttg tatgatggct tgcaaacgat
    40801 ttttgaaacc tatggctact acgcagaaaa aacgatttct gtgacaatga gcggacaaga
    40861 aggttctgcg aaaatctctg ctttgatgga aaaattccgt gaagaagcac caacagaatt
    40921 tgccggagca aaagtcatcc aaacagaaga cttcaaagag ttgacgagaa cagaccgtga
    40981 aggaaacgta gaaaaactaa cgacaccgcc atctgacgta ttgaagtata cgctggaaga
    41041 aaatggctgg atcgctatcc gtccttcagg aactgagccg aaaatcaaat tttatatcgg
    41101 tgtaaaagga aactcttcag aagacgcagc tgaaaaagta gccgcttatg aaacgattat
    41161 ccaaaagatg acagaagaat aaaaaaactt gagactgatg gaaaaagtgg taaactttag
    41221 aagtcagtct tttttcaagt tggaggtgaa tggattgcca gacacgcaaa aaacaaaagt
    41281 agagaaagtc agccaactat tcaaaatgtt gagtgacccc actcgtctca aaattctgct
    41341 gtatctgaaa gacggggaac aaaatgtgac agcaatcatc caagcagtgg aaatggaaca
    41401 atctgctgta tcgcatcaac tgcgtttatt aagagaaaac catgtcgtca agtcccatcg
    41461 tgagggaaaa gcgattttgt atagtcttga tgattctcac gtactggata ttttgaacca
    41521 gacactcaaa catgtggaac atcaataacg ataggtgcaa atagagacaa actggactgt
    41581 gtcaacgaat cttcttcgct gacacagtcc agttttttta aagaaaaatt gttagtctgt
    41641 ttcttcggtg gtttcagttc cataataact atggtcttct aggtctaaag cattcaaaat
    41701 aagcgaagct gtttcttcaa tagataaaga agcgacattg attactacgc agcccaattt
    41761 ttcataaaga tcattcgcaa aatcgagttc agctcgtatt ttttcgatat cggaataagc
    41821 agtatcagga tttaatccgt aagagcgcat ccgttcttta cggatattgt ttaaaatatc
    41881 tgggttattc gtcagtccca cgattttttt aggatcgatc tcccaaagct gtttgggaat
    41941 atgtgcttgt gggatcaaag gtagattggc aacttttaaa tttttgttag ctaaaaacaa
    42001 gctcaaaggc gttttggaag ttcgggatac gcctagcaaa acgacatctg cttctagaaa
    42061 cccacgagga tctttcccgt catcgtattt tacagcaaat tccattgctt tgatccgttt
    42121 gaaatagttc tcattcaata aatgaagggc gccaggttgc cgaatgggct tgataccagt
    42181 taagcgctcg atttctgata tcggcggagt catcacatca aaactaaata attgatgttc
    42241 ttcacaaaaa gaacgcgtca tctcgatcaa ttcttgattg atcaatgtgt gcaggacaag
    42301 agctttttct tctagtgcat cttgcagtgc ctgcatcaat gttttctcat ctgtcacaaa
    42361 tgttctgcgg aataaattga actcaacgct aggatattgg gccatagtgg cttgcgctag
    42421 ttttgtcgca gtttctccag cagagtcgga gatgacaaaa aaagtaacga tttctgtaga
    42481 atcggaagac atggataaaa cctcacttgc atttttttta tgtttattat accatactct
    42541 agaatgtgta cttcgtaatc attttgattt ggaggaataa gtcatgaaaa aacagacgtt
    42601 gatcgaacaa tcactagaaa cattgaaaaa taacggtttt aaatatacta aaaaaagaga
    42661 agcattgtta acttatttag tgaagcgcaa tcgctacgta tccgctaaag aagtttttga
    42721 ctatatgaat caggaattca aaggagtaag ttacgatacg atttatcgaa atctccatga
    42781 ttttgcagaa atcgatttac tggaagaaac cgatttaaat ggagagatga agtttcgttt
    42841 tcattgctgt caaggggaaa tcggccatca ccatcacttt atctgtacgg tgtgcggaac
    42901 aacgaaagaa atccatatgt gtccaatgaa ttatttcgaa gaacagctcg gtggatgtac
    42961 gatcgaaggg catagattcg agattttcgg aagatgtgaa aattgtcaat aaggatttaa
    43021 ataaaataac atttgataat tttaaaaaaa agcaagattt ccttgacggt caaaacagca
    43081 ttagatataa tatttacagg acagtcttgt tttttgagat atatttcaat aacaattacg
    43141 gttacttttt aagctcggag ggagggaatt aacatgtcaa aaacagtcgt tcgcaaaaac
    43201 gaatcacttg acgatgctct tcgtcgcttc aaacgttccg tgtcaaaagc gggtacttta
    43261 caagaatctc gtaaacgtga attctacgaa aaaccaagtg taagacgcaa gaaaaaatct
    43321 gaagcagcta gaaaacgcaa aaaattctag ttctgtgatg aattggagtt ggtagtgtgt
    43381 cactactaag tagattaaac gatgacatga aaacagcgat gaaagcgaaa gataaggaat
    43441 ctttacaagt catccgaatg atcaagtcat ctatacaaaa cgagcagatt aaagaaggtc
    43501 acgacttgac agaagaagaa gaattaacgg tgttgtctcg cgaaatgaaa cagcgacgtg
    43561 attctttgca tgagtttgaa gaagcaggac gtgacgattt agctgaaaaa gtaaaaagtg
    43621 aaattgtgat agtcgaaaaa tacatgccgg aacaactttc cgacgaagaa attcgacaac
    43681 ttgttcaaga agcaatcacg caaactggtg cttcatcaat gaaagaattt ggcaaagtca
    43741 tgggtgcgat catgcctaaa gtcaaaggaa aagcagatgg aaaccaagta aatgccattg
    43801 tgaaagaact attacaatct taattttatc atttaaactg cggacaatca ttgtccgcag
    43861 tttttttgtg ctcgtaaaaa agagatctaa aaaatagcta gacgaaagaa taggattctt
    43921 tcatctagct gtttttttcc tgtttaatat ttcttatgtt aaattgtgtt tgatctggtt
    43981 attcttctgg tagttcattt gcttcttgtt tataccctga taggcgatag gcgtaataga
    44041 agagtaagct gccagcatac ccaagtaacg ttgggaccat ccagccaagt cccaatgcag
    44101 caaaaggaac gtgatgatga taaaattcga gcatgcttgc tacaactctt tgttgactca
    44161 ctaaagcagg cgaatttgcc agcatatcca atacggcggg aacagcagca aacaacatcg
    44221 ttacttgata gacaaatggt gtcttttgga aaaattttgc tgttaaggaa agcaaaatca
    44281 acgcaagcga caatggatac aaaagcatca atactggtaa agaccattgg atgatattgt
    44341 ccaaaccagc atttgcaacg acaaaagaga cgaaagtcgt taaccgcagc catgtcatat
    44401 aactgacttt agggaacagt ttatggaaat cttgtgcaaa agaaacaacg agtcccatag
    44461 cggtagtgaa gacacctaat gtgaccatca ctcctaagaa gctagcgccg aagttaccta
    44521 aataaaaatt agtgatctga gaaagtgcag ttccgccatt ttctgataaa gacatcttat
    44581 tcaaactcat cgcaccgaga agaacaagac caaaatagat catgacttct agcgcaacgc
    44641 tcaggctacc agcttttgct gttaattctg tgactttttt atctttgaaa ccaaggtcac
    44701 ggactgcatg gacaaaagtg acacttaagg caagcaaggc tactgcatca accgtgttat
    44761 aaccttctag gacaccattt gttaaagcgt ttgtttggta agtatctgca gcagattgat
    44821 ttagatttcc cattgggtgg aataaagaaa cgacaaaaat gattgctaat aatgcaagaa
    44881 acaaggagtt tagatacttt ccgacgtatt tgaccaaccg tgtgggatgg acagtaagga
    44941 aataagcaag taaaaagaaa agagcagaga ataaaaacat acctaatgaa ttatattttt
    45001 caggcaaaaa tggtttagca gccatttcaa aagcagtagc agctgtccgt ggtgtaccga
    45061 aaaatggtcc gatcgtcaaa tgaacaagga ttaagaacaa ggcagcatac cattttccga
    45121 caggtttagc taagtcatac agaccatcac tttttgtcac tactacagct aagatagcta
    45181 gtaatgggaa aagagcgcct gagatggcaa atcctacgcc agcactgacc cagtttgcgc
    45241 cagccatttg acctaagtgg acaggaaaga ttagattacc tgacccgaaa aacatcccga
    45301 agataagtga actgaccatc aatagttgtt tccatgaata agtttttgtt tcttccataa
    45361 atttttcctc caataattat cattttgatt gacgatataa acgttgcagc aattagtaat
    45421 gccactaata tcagattcca tgaaaaccac caccttaaaa tataaaaaaa agcccttttc
    45481 cacttgggaa aagggctcta gattctagaa cggtaccacc ttttttcgct taaaaaagct
    45541 tcttcattca cggacgcttt ttgataaagt tatccgctag cctgataatg gaggcagcca
    45601 caatgatcta cgttttcgac attgactcgg aaatgatgtt cgtctattcg atttattgtc
    45661 atttctcact tacatgactc actgggaata aatcgttgaa tagcctactg ttttcctcga
    45721 cgctttgctg tatgtttatt tgttgacgta ataatacacc tgtttttttt gaaatgcaag
    45781 aatattttaa ctattttgtt ttcttttttt gaaacaagaa aaatacctag agaatacctc
    45841 tgaatgaaac ggaatatttt gttctccact ttcatataac ttacattctc agattctttt
    45901 tatcaaaacg agacctttag cgaatggaaa cagctatttt ttaacgtttc ttggtacgta
    45961 tggttaaacg actcttttac aaccttttct tatttgctat atagtttctt ctgagtatgg
    46021 taaaatagga aagaatgaat cgaaaaaaag gggatgtcta tttttgacgg aagagtctag
    46081 ttctttagag attagattga cagctgctga tgatgtcagc atgcttttag gtgcacacga
    46141 caagcatata aaactgatcg aggaaacaac ggaaacaacc atccatacac gtggagaaat
    46201 cattcagatc attggtgaac aatcccagat ttccttggct gcctcagtca ttcggacatt
    46261 acaggaattg atcaaacgag gaatccacgt aagtacacca gatgtcgtga cggcattgaa
    46321 aatggggcaa aaaggtacat tagattattt tgttgagatg tatgaggaag agatcgtcaa
    46381 agaccgtaat ggaaaaccta tacgggtaaa aaacagcggt caaaagaaat acgttgaatc
    46441 ggtagccaaa catgacatcg tatttggtgt agggccagcc ggaacaggga aaacgtttct
    46501 tgccgtagta ttagcaattg ctgccctaaa aaaaggcgaa gtgcaaaaaa tcattttgac
    46561 gcggcccgct gtagaggcag gggaaaacct aggctttcta cccggggatc tgaaagaaaa
    46621 agtggatcct tatctgcggc ctgtttatga tgctttatac caaatttttg gattggatca
    46681 tacgaaccga ttgatggaac gaggcgtaat cgaaatcgca ccattagctt acatgcgggg
    46741 gcggacattg gatgatgcat tcgttatctt ggacgaagca caaaacacga cagtcgctca
    46801 gatgaaaatg ttcttgaccc gtctcggtta tcaatcaaaa atgattgtca atggggatac
    46861 aagtcagata gacttgcctc gagggacgac aagtggtctt gttcatgcag aacgaacact
    46921 aaagcaaatc aaaaaaatcg attttgttaa ttttgaagca agtgacgttg ttcggcaccc
    46981 tgtagttgcc gaaatcatca aagcttatga aaaggcggat cttcatcaag aataagcttt
    47041 gcttaggagg ataagcacat gttgaataaa acactgcaga aactacaaga aaaactcgga
    47101 aaggcagcat tgcctttcct tctgttcttg ttttttatca ttgtctgcgg cgttacattt
    47161 agtagtgtca aacaaaaggc caatgatttc agagaaggcc aagtagcaga agagagtatc
    47221 cgtgcgaata aaacgatcga gaacacggaa gaaaccgaac aaagaagaaa actggcagca
    47281 gaagcagtga ctccagaata tacttatcaa aaagatctgg cagatgacca gaacaatcgg
    47341 atcaagcaat tattcgaatt gatcgataag acgaatgata ccatcaacaa atcttataat
    47401 gacaaggtag ataaggcgaa ggaagatgaa aatatcccga aacctactac agaagagcgg
    47461 attgctacac tgaagaaaaa ttttgaaaac gtgaatgcag aaaacgttgc gttttaccaa
    47521 aaactgccga acaattttta ttcgacgata tttgaaatga ctgaaacgga aattcacacg
    47581 gttagggatg aaagtttgaa gctcattgac gaacagatga gtaaacaagt tcgtgaaagc
    47641 gaattggaag cttttaaaca agaagcagaa gatcagattc agtatttgaa tgtcacaaca
    47701 gagcagcaac aaatgattcg ctacttagtg gatcaaggca ttgtagtcaa tgatgtattg
    47761 aatgagaaaa agacagaaga attaaaacaa tctgcacgag aagctgtaca accagtgatg
    47821 atcttccaag gagaaatcat tgttcgcgaa ggaaatcaaa ttgatgcttc agcgatgaaa
    47881 aaattggaat tactaggatt gacgaatcaa acgacttcga ttttcccact cattgccatg
    47941 gttttagctg ttttgcttca aatcggcgta ctgatttata attcacttca attccaaaat
    48001 gcaggagaaa gaacaaaata cgtgcttttc tatgtgacag ctatgtcagt cagcatagtg
    48061 ctgatgaaat ttttccaatt attccaaacg gaacaagcta cttatatccc gctgttttat
    48121 cccgctgctt ttgcaccact tgttttaagc tttttcttga accgaagagc agggatcatg
    48181 gctggtattt tccaagcagt atctgcactt ttcatttttt atgattcgat tggcacgaac
    48241 tttctaacga ttatcttgat gtcttatgtt ttctctgggc tcatggcaac gattgtcaga
    48301 cgcaaacgga tctcagaaca aggattatct gctgccatgt gggtcattat ttttcctgtg
    48361 atgatggatg tcattttgat cgtctatcaa ggaatgagct ttggtgatgc aacgacttgg
    48421 ttaatgcttg tctgcgggtt cactggtgca ctcttctcat tcttgctgac gatcggtcta
    48481 cacccatata ttgaattgat ggtcaatgat gacagtgtaa ttgtattaaa tgaactcagc
    48541 aatccaaatc atcctttatt gaaacagttg ctggaagaag cgccaggtac ttatcatcat
    48601 agtatgatgg tagctaatct aagtgctaac gctgtagcag aaatcggcgg acgttctttg
    48661 ctaactcgag tagcttgcta ttatcatgat atcggcaaga tcaaacatgc tagctttttc
    48721 gttgagaact tgccttcagg agcggaaaac ccgcacaatt tcttattgcc agaagacagt
    48781 aaacatatta tctttggaca tgtgacagat ggagccaaaa tattggaaga ttataatatg
    48841 ccgcaaatgg tgatcgatat ttgcaaacag catcatggaa ccacgctaat gcgttatttc
    48901 tacatcaaag cgaaagaacg caatccggaa gtaacagaag agcaattccg ttatcctgga
    48961 ccaaaaccgc aaagtcgtga agcagggatc gtcaatattg ctgacagttg tgaggctgca
    49021 gtacgggcaa tggatcatcc gacgagcgaa aaaatcgaaa gttttgtcca taatcttatc
    49081 gaagagcgta tttccgatgg tcaattagac gatagtggac tgaccttgaa agaaatccga
    49141 aaagtggaaa aatcattgat cagcggttta agtagcacct tccattctcg gatcaaatat
    49201 cctaaaatga aaacagaagt tgagaagata aaggaagaac aagaagaata gaaagaagcg
    49261 atgtataatg gatattacct ttatggatga aacaaaccaa gtctcagaag agaaaatgaa
    49321 agaaatcgat gacttgctgc aatttgcagc atcttattta aagctgccag aggaaacaga
    49381 aatgtctgtt actttcatgg acaatgcggc aatccaagta atcaatcgtg attaccgtgg
    49441 aaaagatgca ccgacagacg tcatcagttt tgctctggaa gaagaaggcg aagacgaaat
    49501 cccagtgatt tttgaagatg gagagaatcc tttgcctcgt gtactaggag acatcatgat
    49561 ttctattgaa cgtgctgctg aacaagcaga agcttacggc cattcatatg atcgggaact
    49621 tggcttttta gcggtacatg gattcttgca tatcaacggg tatgaccata tgacaccaga
    49681 agatgagaaa gaaatgtttg gtttacagaa agagatcttg gatgcctatg gactcaaaag
    49741 ataaaaacgt agagaaaaac aaacatttcc tgatgtctgt ggaatttgct gtccaaggag
    49801 tcaaaaccgt ttttgaggaa gaacgcaata tgaggaaaca tgttgcgttc ggtatccttg
    49861 ctttatttat gggcgtgatt ttccagcttg atcggataga gtggttatgg ctgcttttag
    49921 ctgtttttct cgtttggata gtagagatca tcaatactgt ttttgaaaat gtagtggaca
    49981 tgtttacgga ttttcatttt catccgattg ggaaaaaaat caaagatatg gcagctggag
    50041 cagttttagt gaccgcttgt tttgctgtca tcattgggtt gattttattt gtccctaaaa
    50101 tatatcattt gttgtttaat taaaaaggag aacgtatatg ccagaacata aatcagggtt
    50161 tgtcgcaatc gtcggccgcc cgaatgtagg aaaatccaca ttattgaacc gaatcgtggg
    50221 tcaaaaaatt gccattatga gtgacaaggc acagacaact agaaataaaa tccaaggagt
    50281 atatactaca ccagaagctc agctgatttt tatcgataca ccaggaatcc ataaaccaaa
    50341 gcatcgtcta ggagatttta tggtagaagc agcttacagt gcgttgaaag aagtggatgc
    50401 agtcttattt atgatcagtg ctgatcaaaa acgcggccgg ggagacgatt ttattattga
    50461 acgtctaaaa aacgtgcatt ctccagttta tctgattatc aataaaatcg ataagatcca
    50521 tccagacgag ttgctgggaa tcatcgaaga ttattcgacg cagatgcctt ttgcacaagt
    50581 gattccgatc tctgctacag aaggaaacaa tgtcgaacgc ttaatggatg tattagtcag
    50641 tgaaatgcca gagggcccac aatattttcc agatgatcaa gtgactgatc atcctgaata
    50701 ctttatcgtt tctgaattga tccgtgagaa agtcttgttt ttaacacgag acgaagtgcc
    50761 tcattctgtc gcagtcgttg ttgattcgat gaagcgaaac gagaatgata aaatccatat
    50821 ccaggccacg atcatcgtcg aaagagatag ccaaaaagga atcattatcg gcaaaggcgg
    50881 aaaaatgctg aagcaagtag gaacgaaagc aagaaaggat atcgaaaatc ttttaggaga
    50941 caaagtattt ctagaacttt gggtcaaagt ccaaaaagac tggcgagaca aaaaagtcta
    51001 tctgcaagac tttggctacc gaaaagacga atactgatat tctgaacttc ttataggtta
    51061 agtatagaaa taaactggat aaaaatccgc acgttgagaa aaacggcgga agatcgaaac
    51121 ataaataatc tggatcggca gaatctgctt ttccagattt attttttagg agggagaata
    51181 tgagccagct tgcagaatcg aaaggcttga ttttatctag ccgtaattac aaagaaaaag
    51241 ataaattagt caaaatcttt acggaatctt ctggcaaaat gatgttttat atcaaaggaa
    51301 tccatcggca aaatcagcca ttagctccgg ctttgctccc ctttaccgaa gcagtctata
    51361 tcggtaattt tcgaagtgaa ggtttgtcgt ttttaagcag tgtcaaagac gtccatgcgt
    51421 ttcggaacat ccaagaagat atctttttga gcggttatgg tacctatttg ctaaatctag
    51481 tagatgcagc aatcgaagat catcaatatg acccgcattt attccaattt acgcaacaag
    51541 ctctgcagcg gatggatcaa ggagatgatg cagaaatcat cacgaatatc tttgaagttc
    51601 aaatactgca acgcttcgga atcgcgccaa tttggacgca ctgtgtcgta tgcggggaaa
    51661 caaaaggaaa atttgactat tcttccaaat ctggcggtgt gatatgcgag aaacactggc
    51721 caatggacga acaccgttat catgcagatc ctcgcgctgt ttatttcgtt cgaatgtttt
    51781 cggctatttc ttatgataag atcagtggaa tccacttgaa agaagaaacg aagcaggcaa
    51841 tccgtgacct cattgaccag ttgtatgatg aatatgtcgg tattcatcta aaaagcaaaa
    51901 agttcattga tcaaatgaag tcttgggcac acgtattaaa gacgccagag aaaaatatgc
    51961 ctgaaaaaga gatacctgaa aaagaagaat aaatgaaaga tacaacaaaa aaatcaaaac
    52021 tatttgacaa atacagggac ttccgtctat tattaaccgt agattaaata gatcgttcac
    52081 tgagaggatc tattatataa acaagaaaac tgcaaaatga atacgtacat tgaccaagat
    52141 cagtaaaaaa cacgtggttt agcgagttcg ggagagtgga agccggaccc aaacgatttt
    52201 tgaagaggca cttgcgagta cgataaatga agctgccgaa tctttattcg gaagtagggt
    52261 ggaaccgcga aattattcgt ccctatgtct gtaatcagac atagggactt tttgtcgttt
    52321 tctttcctgt attcttattg acagcaacgc ttaaaaattg aaaggggcaa aacagatgag
    52381 taaaaaattg accgtccaag atatgatttt aacattacaa aaattttggt catcaaacgg
    52441 ctgcatgctg atgcaagctt atgacacaga aaaaggagca ggtacgatga gcccatatac
    52501 attcttacgg gcaatcggac cggaaccatg gaatgctgct tatgtagagc catcccgccg
    52561 accagctgac gggcgttatg gagaaaaccc aaaccgtttg tatcagcatc atcaatttca
    52621 agtcgtaatg aaaccttcac ctgaaaatat ccaagagctt tatttagaaa gcttgcgtct
    52681 tttaggaatt gatccattag aacacgatat tcgttttgtt gaagacaact gggagaaccc
    52741 atcgatgggg tgtgccggtg tgggttggga agtatggctt gatgggatgg agatcacaca
    52801 attcacgtac ttccagcaag tcggaggact accttgtaaa ccagttactt ctgagatcac
    52861 ttatggacta gaacgtcttg cttcatatat tcaagaagtt gaaagtgtct atgatctgga
    52921 atgggctgat ggtgtgaaat atggcgagat cttcaaacaa ccagaatatg aacattccaa
    52981 atattctttt gaagtaagtg accaagactt gctactaagc aactttgacc gttttgaaaa
    53041 agaagctaaa cgctgtatcg atgaaaatct tgttcatcca gcgtatgatt atattttgaa
    53101 atgcagccat acattcaatt tattagatgc tcgcggtgca gtatccgtaa cagaacgagc
    53161 aggttatctt tcccgtatcc gcaacatggc tcgtgctgta gccaaaatct ttgtggcaga
    53221 acgcgaaaaa ttaggattcc ctttattgaa taaaaacaca gcaaatccag aaaacatgaa
    53281 ggaggctgaa taagatggca aaagacttat tacttgaaat cgggctggaa gaaatgcctg
    53341 ctcatgttgt cacaccaagc atgaaacaat tagaagaaaa aacagcaaaa tttttagatg
    53401 aaaaccaatt gacttatgat acgatcgaaa cgttttctac accgcgccgc ttagcaatca
    53461 aagtaacaaa cattccagaa cgtcaagcag acagcgaaga agaagtcaaa ggaccagcca
    53521 aaaaaattgc tttggacgca gatggaaact ggacgaaagc agctgaagga tttgtccgcg
    53581 gacaaggtct gacgacagat gctatcactt ttagagaatt aaaaggcgtg gaatatgtct
    53641 atgtaacgaa atacactcac ggcaaagaag caaaagaagt attgacaggt ttagcagatg
    53701 tcattaaaag cctcactttc ccagtaacga tgcactgggc agaatacgat tttgaatata
    53761 ttcgccctat tcactggatc gtggcattgt tagatgacga aataatcccg tttgagatct
    53821 tggatgtcaa aacagacaga gcttcaagag gtcatcgttt cttaggcgaa gatgtgacat
    53881 ttacgcatgc aaaagaatac gaagaaaaat tgaatgaaca atttgttatt gcttcaccgc
    53941 aaaaaagaaa agaaatgatc atcacacaag caaacgaatt agccgaaaaa aatcactgga
    54001 tcattgatct agatgaagat ttactagaag aagtcaacaa tttagtcgaa tatccaactg
    54061 tcttttctgg cgacttcgaa gaaaaatatc tatctgttcc tgaagaagta ctagtcactt
    54121 ctatgaaaga acatcaacgt tatttcgaag tacggaacga tcaaggtatg ttgcttcctc
    54181 attttatttc tgctcgtaat ggcgatcggg taaaattgga aaatgtggta cgaggcaatc
    54241 aaaaagtatt gacggctcgt ttagaagatg cggaattctt ttataatgaa gacaagaaag
    54301 cttcaatcga ccaatacgtt gaaaaattaa aaactgttac tttccatgaa aaaatcggta
    54361 gcatctatga gaaaatgcaa cgggtacaag tcatcgctcg catcataggc gaacaagtag
    54421 gactatctga ggaagaattg atggatctgc aacgcgctgc tgaaatctat aaatttgatc
    54481 tagtaaccaa catggtagga gaattccctg aactacaagg gatcatggga gaaaaatatg
    54541 ctctgctgaa aggcgagaaa ccagccgtag caacagcgat ccgtgaacat tacatgccta
    54601 tatccagtga aggagattta cctgagacta cagtaggttc tgttttagcg attgctgata
    54661 aattagatag tatcttttct ttctttgcag ttgggatgat cccaagtggc tcaaacgatc
    54721 catatgcttt gcgccgtcaa gcatatggtg tcgttcgaat catcgaatcg aaaaaatggg
    54781 cctttccttt gtctgttctg caagaaacga ttagtgaagt tatcagtaaa gatacggatc
    54841 gttttggcat tggcttgtca gcgggacaac aacaggtgat cgactttatc aaaggccgtc
    54901 tgcgccaatt attgacaacg aagaatatcc gtcatgatgt catcgaagct gttttgaatg
    54961 cagaacaaaa agatctgaca aaagtatttg cagcagcaca attgttcaag cagcatcttg
    55021 cagatgaaga cttcaaacca tcgatggaag ctttgacacg tgtcgtgaac ttagcgaaaa
    55081 aagcggagct tgaaaaacaa tcagaagtag accctgaact gttcgaaaac gaagcggaga
    55141 aagaactgca caaagcagta gaggcaatga aaacaagctt tgctgaccaa acgatcaacg
    55201 aaaattatga tcagcttgta agccttcgcc cactgatcga aaattatttt gatcagacga
    55261 tggtcatggc agaagaacca gcagtacgta agaaccgatt aacgcaatta agtcaaattg
    55321 ctgccatggc tttgtcacta gcaagtttag atcaaatcat cacaaaataa tttctaatat
    55381 gtaaataaac cagagaacag gcacaaaata ggctatttc
//
LOCUS       DADVXU010000008        53591 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.008, whole genome shotgun sequence.
ACCESSION   DADVXU010000008 DADVXU010000000
VERSION     DADVXU010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 53591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.008"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(100..666)
                     /locus_tag="KB953_000475"
     CDS             complement(100..666)
                     /locus_tag="KB953_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBB6865477.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(694..2196)
                     /gene="eat(A)"
                     /locus_tag="KB953_000476"
     CDS             complement(694..2196)
                     /gene="eat(A)"
                     /locus_tag="KB953_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HBB6865478.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(2454..3575)
                     /locus_tag="KB953_000477"
     CDS             complement(2454..3575)
                     /locus_tag="KB953_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBB6865479.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3701..4642
                     /locus_tag="KB953_000478"
     CDS             3701..4642
                     /locus_tag="KB953_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBB6865480.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(4792..5688)
                     /locus_tag="KB953_000479"
     CDS             complement(4792..5688)
                     /locus_tag="KB953_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HBB6865481.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            5957..7297
                     /locus_tag="KB953_000480"
     CDS             5957..7297
                     /locus_tag="KB953_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBB6865482.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            7515..7958
                     /locus_tag="KB953_000481"
     CDS             7515..7958
                     /locus_tag="KB953_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HBB6865483.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(8462..9880)
                     /gene="nhaC"
                     /locus_tag="KB953_000482"
     CDS             complement(8462..9880)
                     /gene="nhaC"
                     /locus_tag="KB953_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBB6865484.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(10283..11506)
                     /locus_tag="KB953_000483"
     CDS             complement(10283..11506)
                     /locus_tag="KB953_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBB6865485.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(11537..12376)
                     /locus_tag="KB953_000484"
     CDS             complement(11537..12376)
                     /locus_tag="KB953_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBB6865486.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(12393..13073)
                     /locus_tag="KB953_000485"
     CDS             complement(12393..13073)
                     /locus_tag="KB953_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6865487.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(13075..14148)
                     /locus_tag="KB953_000486"
     CDS             complement(13075..14148)
                     /locus_tag="KB953_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBB6865488.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(14204..14442)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(14485..14718)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(14795..15466)
                     /locus_tag="KB953_000487"
     CDS             complement(14795..15466)
                     /locus_tag="KB953_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBB6865489.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(15553..18231)
                     /gene="mgtA"
                     /locus_tag="KB953_000488"
     CDS             complement(15553..18231)
                     /gene="mgtA"
                     /locus_tag="KB953_000488"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBB6865490.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            18890..19327
                     /gene="ndk"
                     /locus_tag="KB953_000489"
     CDS             18890..19327
                     /gene="ndk"
                     /locus_tag="KB953_000489"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HBB6865491.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(19349..20215)
                     /locus_tag="KB953_000490"
     CDS             complement(19349..20215)
                     /locus_tag="KB953_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBB6865492.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLSHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(20375..22468)
                     /locus_tag="KB953_000491"
     CDS             complement(20375..22468)
                     /locus_tag="KB953_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBB6865493.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(23217..23840)
                     /locus_tag="KB953_000492"
     CDS             complement(23217..23840)
                     /locus_tag="KB953_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865494.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(23999..25396)
                     /locus_tag="KB953_000493"
     CDS             complement(23999..25396)
                     /locus_tag="KB953_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBB6865495.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(25428..26399)
                     /locus_tag="KB953_000494"
     CDS             complement(25428..26399)
                     /locus_tag="KB953_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBB6865496.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            26773..27198
                     /locus_tag="KB953_000495"
     CDS             26773..27198
                     /locus_tag="KB953_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865497.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(27686..28387)
                     /locus_tag="KB953_000496"
     CDS             complement(27686..28387)
                     /locus_tag="KB953_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBB6865498.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(28403..28843)
                     /locus_tag="KB953_000497"
     CDS             complement(28403..28843)
                     /locus_tag="KB953_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HBB6865499.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(28843..29418)
                     /locus_tag="KB953_000498"
     CDS             complement(28843..29418)
                     /locus_tag="KB953_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HBB6865500.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(30266..30745)
                     /locus_tag="KB953_000499"
                     /pseudo
     CDS             complement(30266..30745)
                     /locus_tag="KB953_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(30904..31605)
                     /locus_tag="KB953_000500"
     CDS             complement(30904..31605)
                     /locus_tag="KB953_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBB6865501.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(31713..33122)
                     /locus_tag="KB953_000501"
     CDS             complement(31713..33122)
                     /locus_tag="KB953_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBB6865502.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(33170..35035)
                     /locus_tag="KB953_000502"
     CDS             complement(33170..35035)
                     /locus_tag="KB953_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBB6865503.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(35156..36006)
                     /locus_tag="KB953_000503"
                     /pseudo
     CDS             complement(35156..36006)
                     /locus_tag="KB953_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            complement(36316..37029)
                     /locus_tag="KB953_000504"
     CDS             complement(36316..37029)
                     /locus_tag="KB953_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HBB6865504.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(37097..37423)
                     /locus_tag="KB953_000505"
     CDS             complement(37097..37423)
                     /locus_tag="KB953_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HBB6865505.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(37546..37773)
                     /locus_tag="KB953_000506"
     CDS             complement(37546..37773)
                     /locus_tag="KB953_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBB6865506.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(38095..39348)
                     /locus_tag="KB953_000507"
     CDS             complement(38095..39348)
                     /locus_tag="KB953_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBB6865507.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            39409..40149
                     /locus_tag="KB953_000508"
     CDS             39409..40149
                     /locus_tag="KB953_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBB6865508.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(40235..40993)
                     /locus_tag="KB953_000509"
     CDS             complement(40235..40993)
                     /locus_tag="KB953_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBB6865509.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(40990..42018)
                     /locus_tag="KB953_000510"
                     /pseudo
     CDS             complement(40990..42018)
                     /locus_tag="KB953_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(42038..45205)
                     /locus_tag="KB953_000511"
     CDS             complement(42038..45205)
                     /locus_tag="KB953_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865510.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(45429..46313)
                     /locus_tag="KB953_000512"
     CDS             complement(45429..46313)
                     /locus_tag="KB953_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HBB6865511.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(46408..46845)
                     /locus_tag="KB953_000513"
     CDS             complement(46408..46845)
                     /locus_tag="KB953_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865512.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            complement(47062..47973)
                     /locus_tag="KB953_000514"
     CDS             complement(47062..47973)
                     /locus_tag="KB953_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBB6865513.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(48430..48858)
                     /locus_tag="KB953_000515"
     CDS             complement(48430..48858)
                     /locus_tag="KB953_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865514.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(48886..49524)
                     /locus_tag="KB953_000516"
     CDS             complement(48886..49524)
                     /locus_tag="KB953_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6865515.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(49528..51510)
                     /locus_tag="KB953_000517"
     CDS             complement(49528..51510)
                     /locus_tag="KB953_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HBB6865516.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            51690..52355
                     /locus_tag="KB953_000518"
     CDS             51690..52355
                     /locus_tag="KB953_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6865517.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
BASE COUNT        15573 a        11079 c         9028 g        17911 t
ORIGIN      
        1 gagtttatat tatcaaaaga attgtcataa cctaaaaaag gcactgctaa gcaaaagcag
       61 tgccttgaaa tattttattt aaataacaca aaaacataat tactcataag aaaccaattc
      121 tgtattatct accatgaatg taaagaaccc ctcattaggc atcggatgaa aataagcatt
      181 gatcactctt atcaaggcat tatggcaaac taacagatag acattttctt cgtcttcgat
      241 gcattctttc aataaaggaa cgatccgtgc atacacatct aagactgact ccccatttgg
      301 gaatcgtaca gcgaacgcta aacgtgcttt ttgaaagttc tcatctttgc ttggcagtcc
      361 atcataatcc ccaaaatcca tttctaccaa tcgttcatca acggtcagcg gcaagctgag
      421 acgatcagca actgcttgtg ctgtatctct tgctcgttga agaggggaat ggatgatttt
      481 cgtgattgga acaggcaaat ctactacctt ttctgctagt ttctgtgcct gcatctgtcc
      541 tttttcagtc agcggcacat ctgcccgtcc gcaaactaat ccttgtgcgt tccaagttgt
      601 ttctccgtgt ctcgttacat aaagcatgtg attctctcct tcgttggcaa agttcccctt
      661 aaccatacct tgttgttctt gagattacaa gggttagatt ctctcaagag aaataatctc
      721 agtagatact ttatccagga aggtttgatc atgttccact agtaacatgg caggtttcac
      781 gttcaagatc agttgttcta attgttcttg attgaagaca tccaaataat tcaatggttc
      841 atcccatgta tatagttcag ctggctgtga taaagattta gccaattcca cttttttccg
      901 ttggcccata ctcatctgct cgatcttgtt atgaaaaaca tctctttcca tcccaagctt
      961 tcggaggttg ttcaaaaatg cttggtagtc tacttggttt ttctctgcaa attccgccaa
     1021 cgttcctcga ttgtcttcat aattttggct tgcataacta atgctcagat gttttggctg
     1081 gtatttttct cctataacct tgccgttgaa tgcccccaga agataatgga taatggatga
     1141 ctttcctgca ccgttaggac ctgaaatcgc cacccgctga tgaggctcga ttgtaaaatg
     1201 aattggctcg aataacagat tttcataccc taattgaaga tcttctacgc ttaaaagtcg
     1261 cttatggtga gacgcttggc tattcatcgt caaagaatcg atatattcga tatcttttaa
     1321 tagtttttct ttctcgctga tctgggtctc catccgattc acgattgctt tggatcgttt
     1381 catcgtccgt gcagcatcag cacccactgc tcctttattc actcgtctag attcagtatc
     1441 gatgaatccg acttgtttct ttgttttatc tccttctcgg gaacgagacc attcggcttt
     1501 ttcagctgct gttttcttta gcctactgac ttcttttttc aatttttcat tttgagccat
     1561 ttcaaattca tcacgaagtt ttttctgttc ttcatagata gagaaattcc cttgataaag
     1621 ttccagttga cttttttcga ttgctaaaac atggtccact acttcatcga taaatccccg
     1681 gtcatggctg accacgatga agccttgttt tttctttttc aaataagccg ctacttgttt
     1741 tctaccagag atatccaaat gattcgttgg ttcatcgatt aacgggaaat gagtgtcatc
     1801 cacaaataaa agtgccagta ggacttttgt cttctctccc cccgatagtg tgctgaattc
     1861 tctccataag atttcaggat ctgtttgcat caattggagc tctctttcga tttcccatat
     1921 ctcaaaatcc gtaatatcat ttaacacgta ataggttaaa cgttctttat cttttgtctg
     1981 ttgcgggaaa taggcaaatt cttgctgatg gattacttgc ccttgataag gtagtttgtt
     2041 ttgtagaata ttcagtaaag ttgtctttcc tcgaccgttt cgtccgataa gtcctagttt
     2101 ccattgtgtg tcaaaattta gatttgcttg ttcaaataat aatgtgcctt ggctgtcgta
     2161 gccgaatgtc agatttttta tttcgatttt agacattttc catcacctcg taaaaaaaga
     2221 ccagtcctat tttcagaact ggctaaagag gatgcaaggg ttggatacac acaaaaagac
     2281 ccatacatcg aaaaaaccaa gccaatcccg aaaatctgca cactgcttcc ttgtctccac
     2341 aaggtacagt ctgaatgcag ctttcatcaa tcgacttaat ttttcaatgc acggatccct
     2401 cactttgtcc tatatttgaa attacgatag cataaagaaa atgattttgt caactacttc
     2461 gggaggatgg tgaaaaatct gctaagtgtc tgtcctaaga tttttgtttt ttctaatgtt
     2521 tgcctgtgtt ccattgcttc ttctaaacct attggaccca aatgaatact taagacaatt
     2581 gggagaaaat cagaaagtcc ttctagctct tggtctctac ttccgcaaat agcgattatt
     2641 ggttttcgat atttttttgc aagcactgct acaccgtatg gaacttttcc gtggatcgtt
     2701 tgctgatcga tccgcccttc tcctgtaatg atgacatctg cttgttcgat ctttttttcc
     2761 aatccagtga tttggctgac taaatcgaaa ccagaaacca tcttcgctcc caacaaaaaa
     2821 agggctccac caataccgcc tgctgctcca cttcctggaa tatcatctaa atccacctcc
     2881 cagaaatctt ttatttgctg agcaatcttt cctgcttgtt tatctaagaa agctagcgtc
     2941 gtatctattc ctcctttttg tcttccaaat tgccaagcag ctccttgttg acctgtatat
     3001 ggattggtaa catctgcagc gataatcaat tttactttag aaacaagaac gtttcttcga
     3061 tcaaaatgaa gttttttagt agataataaa ggatttcctt ctgtgtaccc ttctaaagaa
     3121 gcacccaagg attggagtaa tcctagacca ccatcgctac acccgcttcc tcctaaagtg
     3181 acaataattt gttctacttc ttgaagcagt gcatctttaa taagttctcc taaggcataa
     3241 ctggaagcaa gtcggatagt ttgatccgtt ggtttatcaa gtagatctaa ccccaatgct
     3301 tgtgcagatt cgatgactgc ggtgtttttt ccttgccaag ttgtcaataa atagctgatt
     3361 tttcgctgac ggaataagag atccatccca tcaatggtac gccatgttcc cctgagcgcg
     3421 taatagattg cttctgcagt attttctccg ccatctgcaa tcggcacatt gatttttctt
     3481 cctttacgat cttcccagcc atttagaact gcctgattta gttcaaatga agaagcactt
     3541 cctttaaatg aatcaattgc cgccaaaata tccatctgta tccctcactt tcttcttaag
     3601 taagacactt tttttcattt ttttcaaaca aaacaatgct attttcttcc atttttctat
     3661 atactaaaag ggaactcatt gaataaagga gagaaaaaac atgaaaatcg caatagctgg
     3721 ggccggagca atgggcagcc gaatcggatt gatgctacat caaagcggaa atgaggtcct
     3781 cttgatcgat cgttggcctg ctcacattga agccattcgt acaaatgggt tgattgctga
     3841 ctttaacggt aaagaagtgg tcgcaaaact gccaatctat tcaccagaag aaatcatcga
     3901 aagcaacgaa cacgtggact tgatcgtagc attaacaaaa gccaatcaat tagatgatat
     3961 gttttgttcg atccaatcca ttattactga caatacttat gttttgtgtt tactaaatgg
     4021 attaggtcat gaagatgtac tggaaaaata tgtgccaaag aaaaatatct tatttgggat
     4081 cacgatgtgg acagctggtc tagctggtcc cggaaaagtc acattgttag gtgatggaga
     4141 aatcgaacta gagaacttgg aaccagaagg cgaagcattt acaaaaaaag ttgtagaagt
     4201 ctttcaagaa gcaaatttaa atccgatcta tagccataac gttcgttatt ccatttggcg
     4261 aaaagcttgt gtaaatggaa cattgaatgg tttatgcacg attttggatt gtaatattgc
     4321 tgaactaggc gcgcaaaaag cggcagagag catggttcga acaatcgtat ctgaatttgc
     4381 ttctatcgca gcaaaagaag gaatcatact agatcaagaa gaagtgtacc aacatattgc
     4441 ttctacttat gatcctgaca acatcgggtt gcattaccca tctatgtacc aagacctgat
     4501 caaaaaccat cgtttgactg aaatcgacta catcaatggt gccatttggc gaaaaggaca
     4561 aaaatatgat attgctacac catattgtgc ctttttgacc caattagtcc atgcaaaaga
     4621 aggaatcctt ggcgcagaat aaaaggctct ctgatgagaa tcagctgaat cagcagctgg
     4681 ttctcttttt ttaagcccaa taaaagatca aaaagaaaac acaactctca tgatgccata
     4741 agaattgtgc ttgtttgttt cagtacataa tctcttcaaa atgtaggttt tttatttgtt
     4801 ttcaacagag gagaggtcat attcgtattg ctcataaata gtggaatcgt tagtgataaa
     4861 tcgttttgcc caattttctg catcataaaa ttccaatagc tctgatagga ttggcttaaa
     4921 tggtgtatgc agaaattccg gtaattcatc taaatcacgt tctcgaagca aaaaaatatt
     4981 ttcaggacga taaaaatgat aatgagcaat cgcttgatta tccgtcacta gtttcttttg
     5041 aaaatacatg gattcaaatg tccgcaaagt cactccttgc tgaccctccc gcaacagttc
     5101 cagaatcccg cgcgattccc ctgttttttt caggtactct tcataagtaa gatatccagg
     5161 ttgttcattc cctcgatgtg gaagaatgaa atagttggtc gtaacatctc tttttataaa
     5221 ttcctgctta tagctttctg ctcgttcttt tctcccatta ttcgttgcac caaaaaatag
     5281 atccgtcttt ggttcgctag aagaaaaatt catatgtttc gaataaaatg aactattatg
     5341 tttcagccca agttcttttg cttccaaaaa atcaaaatga taaatctcat cgattgccgg
     5401 ttcatcttgt gcctgcttca gcatcgcctc gtgttctgct ttgaagtgat tccaaaaaaa
     5461 taacacaatt tttgcattgg taaatgggcg cacgcgtcga gccatctcca tatcaaatcc
     5521 ataatctaaa aaaacgatca gctgataatt tgccaactgt tgtctagctg atttaatgaa
     5581 ttccgactgt agtaaatctt ttccggacca aacatctaaa gagcggatgg aaggcaaaaa
     5641 ataaagtgat ttattttttg caactagcag tacgtcattt ttgttcatct tttccgcctc
     5701 actttctctg tcactttttc ctatgatagc ctgtttaaat agtaaaaagc cagtaaaacg
     5761 tttcctatat ttgtatgaaa atttaacatt tgtatgaaaa tatgatgaaa taaatcattc
     5821 ttctattaaa attcgcctaa aagttttctg aatagttgta attgtctgaa aatttgttat
     5881 cattagtcat tgttaaatat tgctatttat tcttaatata aattttgatt gtgaatttaa
     5941 aagaaagagg tcatttatgg aagataaaaa attgaaaaaa caacttagct ccagacatat
     6001 caccatgtta gctttaggtg gagcaatcgg tgcaggtcta tttaaaggaa gtggcgaagc
     6061 gattgggatc gccggccctt ctgtattgat tgcttttttg attggtgggc tgattttata
     6121 tatcgtgatg aaaggtctgg gaaaaatcgt tttaagcggt ggcgacacgc atcacggatt
     6181 atctggtttg atacgccctt atctaggtgc tcattctgct gattttactg attgggtcta
     6241 ctggtctatg tggattatca atatcatcgc tgaagctgtt gcagctgctt cctttttgca
     6301 gttgtggttc ccgcacattc cagcctgggt atttgttttt atgttggctg ttttaactac
     6361 gatcatcaat ctttattcgg tacggttatt cgcagaaaca gaatattggc tggcttttgc
     6421 taaaatcagt gtcattatct tactgatcat ttttgctact tatcttgtcg gtcagcaaat
     6481 gttgggcaca ggcgttttcc ctacccttca gcaattgact gaccatggtg ggtttactcc
     6541 tcatggtatg aagggaatca tcagttcttt gcttgtagtt atctattctt acggcggttc
     6601 tgaactgatt gctatcacgg taagcgaagc agatgatcct aaacgtgcga ttcctaaagc
     6661 aatcaaaggt gtcatgggaa gaatcatttc cttttacatc attcctttgt ttttgctttt
     6721 gattcttttt ccttggaata ctttagcagg cacgaatgtc agtccttttg tgatggtatt
     6781 tgaaaaaatg aatatcccat ttgcagcgga tatcgtcaat tttgtgattg tccttgcttt
     6841 gttctcatct atcaattcgg gtgtgtatgc atcttcaaga attttgttct tccgtttgaa
     6901 ggatcgaaaa ggttcctcta gaaaactagc tgttttaaac aagcatcaag taccgcaacg
     6961 tgctgtttta ttttgtacca gtgttcttta tcttggtgta gctctttctt attttgtcgg
     7021 tgacaaatta tttggctatc tggcaggttc cctttcgtat acagtattat tgatctggat
     7081 cattatcagt gcggcatcct ttgtactggc actaagaaaa ggaacattat gggataaagg
     7141 aatcagcttt tttgcattag ctgtcttagg attgattttc cttggtatcc tgttcacgaa
     7201 ctccattggg gtaactgtat tgactggcct gctttacctg ttcatttatt tcagttatca
     7261 aaaaaagaat gatgcgttcg tattgacgaa tgaatgaaat cgatagaaaa ttttatttta
     7321 tttatgtgga atagatggag ctattgaaga aaggagttgt gacaaaagtc ttgttgcaac
     7381 tcctttttcc gaataaacag tctcatgacc tcttggtaaa cggtgttcaa aaaccctctg
     7441 tgcaaaataa ttgattgttc tccatgctca tgacatgata gaagaaagga atgaaaaaaa
     7501 ggagtttgtt ttaaatgaaa aagtattgga agtttttgtt gatgatcggt gtttcaacgg
     7561 tagttatgtt tggtatgatg tacttgaatg tctatgcgct cgatcatatc ttcttcagtc
     7621 gtacacgagt attcatggcg ttgatgatgg gtgcagtcat ggcagtcatc atgctgttgt
     7681 tcatgtggaa aatgtatgaa aacaaaaaga taaatgttat gatcttagcc gctagtcttc
     7741 ttgtttttgc cggttccttg tttatggtac gaagccaaac tactgtcaac gacacagctt
     7801 ggatgaaggc catgattcct catcattcta tcgctatcct ggcaagtaaa cgagctgatt
     7861 taaaagatcc tgaagtaaaa aaattagcgg aagaaatcat tcaggcacaa gaagaagaga
     7921 tcgacaaaat gaaaaaaatg atcaaagaaa tggaatgacc attatccgtc caaataaaaa
     7981 aagaggatgt tccagaagtt atcagcttca agaaataaga agaagctact tctggaacgc
     8041 cgtgaataag gggttgtgaa aaagctgccc tgcatcaagt aataagaaca gccaaacaaa
     8101 aatagcttct cctatttttc gtttggttag gcttattacc gcagatgcaa gcttttgaac
     8161 accgtttatt aagaggttgt aaaatcagcg ttctgacctg agtattagag agacaaatgg
     8221 aaaaatagtt cctcatattt tgccattttt tgagctaata ccgagggtca gctgattgaa
     8281 caccgtttat cgagaggttg tgaaaaagtc gcttagtttc gcgtattaag aaaaattttg
     8341 tgaaatggct ttccacattt ttgtataaat ttggcttaat accgagaaac tggcttttga
     8401 ataccgttta ttcgtaaaag ggattgtgac aaaagtcttg tcacaatccc tttttatgtt
     8461 tttattgttc taattgcgct gtcttgctta ctgcttcttg cggtttcaca aatttttttg
     8521 agattcccca acctgtgaag gcactgacca tcgaaaacac aggagaaagc aaactgaaaa
     8581 atacgaatgg caagtaagtg agtgtaggaa taccaaatgt acttgctacg aaacttccgg
     8641 caattcccca aggcactaag tagttgatta ctgttccacc atcttctagc gttctaccta
     8701 atacagttgg atcaaatcct gctttttcat aagtttcttt aaatgcatta ccaggcagaa
     8761 taacagatag gaattgttca ccaataaaaa tatttactcc gatacttgtc atcaatgttg
     8821 taaaaatcaa atttggtgtg ttcgtcatct ttcttgatac tcgttccatc acggttgaga
     8881 tcaagccaaa ttcgatcaac aatccaccta atgacaaagt tagaataatc aatgacacag
     8941 taggcatcat actttcgatt cctccgcggc taagcagtga atcgatttgt tggtttccag
     9001 tatttgaaac aaaccctgat tcgatgaccc cagccaactt cgttaaggaa gtttgtgggt
     9061 tttgaataaa aatcattcct actgctacag cgatattcaa caaaatcgtt aaaattgccg
     9121 gcaccttttt ccatgcacac agtaacatta aaaataaagg aatcagtgac cagattgaaa
     9181 tagtaaagtt tgcttctaga acatcagtca cttcacgaat agatgaaaga cttgcggctt
     9241 tacttgtgtg tcctaaaatc atgaataaaa agaaagaaac aacaaatgcc gggacagttg
     9301 accacatcat atgcttgatg tgtttgaaca aatcggcatc aacaatagct gctgctagat
     9361 ttgttgattc ggataatgga gacatcttat ctccaaaaac ggcacctgaa acaatagctc
     9421 caacaaccag cgctggattt aagcctagtg ttgtcccaat accgaaaaat gcaatcccaa
     9481 tcgttgacat aacagtaaag gcactcccta cagcgctacc tacgattgca cacacaacaa
     9541 atacagaagg gacaaaccac tggacactga tcaatttgaa cccaatgacc attagagttg
     9601 gaatgatccc tgcttgtatc catacagcaa tcaatgaacc gactaaaata aaaataaaga
     9661 ttgggatgat tcctggtttg atgccattga tgattccttc gtgcagccaa tctgtcgggt
     9721 atctttttat cactgcatac aacatgacca ttccgatcgc tgctaagatc gtgatattag
     9781 gtgagacctt taatccgaca acgccaatcg caatgcctaa aacaataacc attaatacaa
     9841 gtgttgcttc taaaaaactt ggtgttcctt tttttcccat gataatctct ccttttttag
     9901 ttgttcttat gttctaaaaa agttaagata tcggattact ccgccgcaat tcatccgcga
     9961 ttcctccttt ataaaaataa aaagacctgt tttaaactaa ggacgaatga ctccgcggta
    10021 ccaccttaat taaaaacaga tctgtttttc actttcaaac ttttattcaa atgtatggac
    10081 tttccatgta attcgtcaat taggcttgat cggttcacag cgaccaccga ctttctgaac
    10141 aaaacaccta accgctactg cgtgaaaagt tgacttgtac gaagtttacc ttccataaga
    10201 tcatttgtca atgaaaaaga aaatattttt tcatttttct gaaaattatt tttataactt
    10261 tcacacacaa gtttattttt tactagaaag tagcgccctc ttgttttttt cttgctacta
    10321 tttcttgatg ttcttgcttg attctttcta atagctttgg ctctttcaac agatcaagag
    10381 ctgtattggc gagtagttct gctgcaatcg caatagaagc aaggcctttg tcacttttag
    10441 cagcagcttt gaattcttct gtgtgtccgg caatatatgt ctcagagatt gctaccgtag
    10501 gctggattgt tggtatgact tggctgacat ttcctacatc agaagaaccg agacttgttc
    10561 tttgttccgt ttctatttct tcatctggaa cacctgcttc ttttgcatgc tggaagaata
    10621 agtcatcaaa agaaggagta acgatgacat catcaactgc attttggaac aatccaaact
    10681 caaaagtcgt gcccgtactt atcgcagcac ctcgtacgat attttccact ttttcatata
    10741 ctttatctaa tgtttgccga tttcctgcac gtaaatagaa acgtccagca gcgtaatctg
    10801 gaatgacgtt ggcagctact cctccgtctg taatgatccc atggatattg acatcttttg
    10861 gcaattgcaa gcgtagagcg ttgattccat taaacacttg gatcaatgct tccaaagcat
    10921 ttactccttt ttcaggtgct gctgctgcat gggaagcgac accgtaaaat ttgatgtcga
    10981 ctggatcatt cgctaaagat tctgtcgttt tcccataacg aaaagctgga tggacgcata
    11041 acgctacatc gacatcctca aaaaaacctt ctcgaacaaa gctgccttta gctgatccgt
    11101 tctcgccccc ttcttcccct ggggtgccat atacacggat ttctccacca gtttcatcaa
    11161 tgaattgctt cactatgctg gcggctaata cggagtaagt accaaacaaa ttatgcccgc
    11221 atgcatgacc aatacctggt aaagcatcga attctgccaa aaaggcaatt gtgggaccgg
    11281 gtttattgga aatataacgt gcatcaaagg ctgttcggtg tccagctact tctttttcta
    11341 cccaaaacct ttctttttct aattgtttaa tcaacgctgc tgaggaataa aactcatagt
    11401 tacttacttc aggatgttcg tggatatcta gagcaagtgc ttgatattct ggtaaacgat
    11461 attggattag atccttcaat tggtttgttg agattgttgt tgtcataaaa aattcctcct
    11521 atgctagtca tttttcttat tttctcccga atgtttccca tgcaggcacg cttgatcctt
    11581 ttgatgtttt ctcgattact ttgactgtgt cttctgtttg ataagcgtct actatttttt
    11641 gatacgtttt attgttttca tcttcttttc ttgcaacgat gatattcaca taaggacgtg
    11701 catggtcatc cactggttct aagaagatgg catcagaagc aggattaaat ccagcatcta
    11761 cagctacacc gctattgatc actgaaatgt ctacatctga caaagcacgt gctgtttggg
    11821 aagcatctaa ttcagaaatg tctagatttt ttctattctc ggtaatatca gaaacagtgg
    11881 gcgcttgtcc ttttgcctta tccactttga tcaatccagc agtttgcaat aacagaagag
    11941 ctctgccgcc atttgtaaca tcattaggga ttgctacttt tgctccgtct ttgatatcag
    12001 ctacatcctt caaagtgtta gaataaattc ccagcggtgc attgacagta tttccgatcg
    12061 atactaaatc agttccgtgt tctttattgt aattgtctaa aaagaattga tgttggaaag
    12121 aattcaaatc aatcgatcca tctgctaaag ccacatttgg ttgattgtaa tctgtaaatt
    12181 tcacgtattc caaattaatg ttctcctttt tcaaacggtc tttcacgttg tcccatacat
    12241 ctgtatcttc tccaataatc cctagtttta cgatagtttc ttttttgtca gttgtatctg
    12301 cttttccttg gctacaccct gccaaccaaa taattgctac aaatgtaatg actacaaaac
    12361 gaataatttt tttcattttt tctcctccac tttcaatgtg taattttttt aatcagttga
    12421 ttactgataa attgacttaa aaaaactaag attagaatca ataatgtagc cacaatcgtt
    12481 acatctgttt ggaatcgatt ataccctctc gatatagcaa gattccctaa tccgcctgca
    12541 cctactgctc cagccatggt agtcagtccg atcacgttga tgatagtcaa cgccgagacc
    12601 cgaatgatac caggcaaacc ttctaacaaa tacacacgta aaatgatacc gattggactg
    12661 gttcccatcg cttctgccgc ttcgatcacc ccaggatcaa cttctagcag tgcgttctcg
    12721 atttgacgag caaaaaacgg aacaactcca aaaatcaatg gcactaaagc tgctttcgca
    12781 ccaatcgtcg ttccaactaa gaaacgagta gtcaatgcta aaagagaaag caagataatg
    12841 aaagggatgg aacgaatgat attgatcagc ttatcaagta tttcaaatag cagacggtta
    12901 gctagtaatc cattagggcg tgtcactact aacgtcacac ctaagatgat tccaatcatc
    12961 ccgccaatga atgctgtcca aaaagacata tatagtgttt gcttcgtcgc ttcgacaaat
    13021 tcatccggaa tcaaataaac attgggcaaa tatgttttaa agaaatcagt cattttatct
    13081 tcctccttcg atcactttga tacgactatc taaatcgttt ttttgtaaaa tatttatttt
    13141 tacaccagat ttgatcaaat aatccaatgc ttttttccgc tgtttggttt ctccagctaa
    13201 agtaacgatc aaactcccta acggcgtttc ttgtaataac tctacattgc catataaaat
    13261 attcgctgtt acttgatatt tgctgtacag atgagcgatt agaggttcgt tcgtctgatt
    13321 tccaatataa cttaattcca ccagccattt attggcgatc tgatcggcaa aagcagaatg
    13381 ggcaatgatc gtttctaatg cttggtcaag atgagtagct gttcgaataa aatctttggt
    13441 caattcttct tcaggattgc tgaagatctg gatgctgcta cctttttcaa cgattcttcc
    13501 atcttccata accgctacct tgttgcagat ttctttgaca acttgcatct catgagtgat
    13561 caggacgatt gttaaaccta actggcgatt caattttttt aacaatgcaa ggatctgaag
    13621 ggtcgttttc ggatcaagtg cactggttgc ttcatcacat aataatacct taggttcatt
    13681 cgccaaagct cgtgcgattg ctactcgttg tttttgtccc cccgacagtt gtttaggata
    13741 agcagatgct ttttcttcca aaccgacaag ttccagtagt tcattcactt tttgacgacg
    13801 ttcctgtttc gattttccag aatactttaa agaaaaatca acgttgtcaa aaatagttcg
    13861 gctttccatt agattgaaat gctgaaagat cataccgatg gcctttcttt cttttcgcaa
    13921 ttctttggga gagagctgac tcagattcgt ctgattgaca atgacttctc ctgctgttgg
    13981 tttctgcagc aaattcatca cacgaactaa cgtacttttc cctgcacctg agtaaccgac
    14041 gattccataa acatcgcctt tttcaatcgt taaatctaca gaatctactg cctgtatctc
    14101 tttttctttt tgtttgaaac atacagaaac atctcgcaat tcaatcatta aaattcctcc
    14161 ttatctaaaa aaagcgtcct caagaaaatg aaaatgtcat tttcttgagg acgctttttt
    14221 gcgtgttacc acctcatgtt caagctgtgt tcacacacaa ctcctcttca agtacgatca
    14281 tactctagca agatatcggt cgcagccgtc atcgccttac atctatgctc gggatgaatt
    14341 gctcgatggc cattttccaa tagtcgttcc ttgcttcttc tcagctaccg aagctctctg
    14401 tcaaaggttc ttctatcgta cttaccattt caatgcaaat aatattcaaa aaaaaagtcc
    14461 ttttactaac ataaactatg tcagtaaaag gacgacgtat cgcggtacca ccttttttcg
    14521 gaagtttcct tccctcaagc ccaacatctc aaaaagagag attggcgttc gttttaacgg
    14581 tcgaacagtt ccgaatttgc ctacttatcg acaaattatt ctccaaggcc attttcagct
    14641 tttcttactt gactcttctc accagccaga gcctctctgc aaagtttcca aacttacttt
    14701 ccttttcaac gaaatcgata ttttgttgtt gggattaatc tacatgagga aaactgaaaa
    14761 gtcaagtacg aaattcaaat tattttttct atatttattt ttctgttttt ttatttaaca
    14821 tttcaataac agttcgaaga tcttttcctt ccgcaagaag tttcaatttt tccttttctt
    14881 cgacggtata atttcggaca aaactggaat caggtatcaa aacaacgcct aatcctgctc
    14941 gtgaagcagc tgttgctcct actaaagaat cttccactgc aaaggctgaa ctagcttcta
    15001 atcctgttcg ttccaaagct gtcagataag gaatcggtga aggcttgttc tccgacactt
    15061 cgtctccgaa tgtaaacgtg tccaagtaat cagctaattt aaagtgatcc aagatatctg
    15121 ttgctctttt cgtaacagtg gtcgtagcaa tgcctagttt catatgacgt tcttttaagt
    15181 ctgccaatat ttcttttgca tatggtttta agtggatggc tccgcttgtc agctgttgat
    15241 aaaaatattc ttcacgcttc gctcggactt cctgtacttt ttcagggcta cctgctgctt
    15301 tcactaaaat atcaaaactt tgtttccagt tttttccgcc ccaagcgtca acgatttctt
    15361 ttgggatatg gatatcaaat tgctgaaatc cccaaagcca gccatcacga tacgtttttt
    15421 ctgtgtcaac taacaaacca tctaaatcaa atattgcacc tttcatctat acattaatcc
    15481 tttccaaaac aatacacata gtttagcacg actggtccaa aaagaacaga agcaaagaac
    15541 cagccttctc gtttataaga gttcttggaa tcgacggata tagattttct tcacagctgt
    15601 cacaagcgcc agataagcga tgactgtagc aaataaccat aaccagtaat tgccaggaag
    15661 tggcatcaag cctaattcag caccaaaact tgtaaaagga aggacagaac cgacaataat
    15721 tcctaatgta gtgataacaa acatggcaaa tgttgcgttg ctttggataa atggtacttt
    15781 cggtgtacgc agtgcatgga gcactaaggt ttgtgtccat agtgattcga caaaccatcc
    15841 tgcatggaat aatgcgatga aaatcactcg ctgtgaacta tccaatgtat ggaaacttcc
    15901 gcccactaca gcaggacaaa taacaaaata catcaacaag taagtagtaa tatcaaatat
    15961 cgaacttgtt ggaccaaagt aaaccatgaa ttccccaata ctagatgcat tccatttttt
    16021 tggctcgtcc aaatattctt tatccatccg atcccatggc aaggaaatgc aggaaacatc
    16081 atagatcaga ttcaaaaaca aaagttgtaa aggcagcatt ggtaaaaatg gcaggaacgt
    16141 acttgcaatc agcacggaaa acatattgcc aaaattagaa cttgctgtag ccttgatata
    16201 tttcatgata ttcccaaaag tttctcgtcc agaaagaagg cctctttcca aaatcgtcaa
    16261 atctttttct aaaaggatca cgtctgcaga ttctttcgca atatccacag ctgtatctac
    16321 agaaataccg atatcagcca ctttcattgc aggagcgtca ttgataccat ctcctaaaaa
    16381 tccgacagta tgaccatttt tacgtaaagc tgtcgtcaaa cgtgcttttt gctgaggatt
    16441 caattttacg aatacttcat ggtcttcagc tacttgtgct aattcatgat cactcatttc
    16501 cgagattttc tcaccggtaa ccaattcatt gatctctaga cctacttgac ggcacactga
    16561 acgtgtaact aattcgttgt ccccagtcag aacttttaca gctacaccat gttctttcag
    16621 agctttcaac gcttgtttcg ttgtttcttt tggtggatca aggaaagcaa gataaccaat
    16681 caaaaccatg tctgactcat ctttgactga aaattctcca acgacgcttg gattggtctt
    16741 ttgtgcgaca ccaatcactc gtaagccatc ttcattaagt gactgtactt ttgcaagtac
    16801 ggcttctttt cgttcttttg ttaatggtgt aacagtaccg ccaagatcaa taaatttaga
    16861 aatgttcagc atttcttcaa tcgcgccttt tgtgatcatt tgtgttttac cagctggatc
    16921 agcaaccaca acactcatac gacgacgttc aaaatcaaaa ggaatttcat ccactttttg
    16981 ataattgatc ttatctgtat ctaaagtttc tgttgcttca tcaatgatag ccacatctaa
    17041 caaatttttc agccctgttt gatagtagct atttagatac gcatggcgca aaacacgatc
    17101 atcttcattt ccttcacaat ccatatgata ttcaagaatg attttatctt gtgtcaatgt
    17161 ccccgtttta tccgtacata acacatcgat tgcaccaaag ttctggatgg cattcaaatt
    17221 tttgatcact gttccttttt tggccatcac acttgctcct ttgaccaaat tcgtcgtaac
    17281 gatcattgga agcatttcag gtgttagacc aactgctaca gatagtccaa aaagaaaagc
    17341 ttctagccaa tccccttttg taagcccatt gatgacgata actgctggag ccatcaatga
    17401 cataaatttg atcagtagca tggatgtttt atggatgcct aattcaaagc ttgattgaat
    17461 tggtttttca gatagcgttt ctgctacatg accaaatagt gtttcatttc caacagaaac
    17521 aatgattcct tccgcacttc cactgatgac attacttccc ataaagacaa gattttcgta
    17581 acttgtttcg ctttgctgat cgtcttcaca tactttcgca tgcttttcta ctggatagct
    17641 ttcccctgtc aatgctgctt gggaaatgaa taaatccttt gcggacaata aacgcatatc
    17701 cgctggtatc atatcccctg ctgaaagccg aacaagatct ccacagacga tttcttccat
    17761 aggaatctct gtgtactttc cattacgttt tacagctgcc gttactttca caagtgattt
    17821 cagtttttca gccgcttgat tggaacgtac ggactgaatc aacgtcattg tcccgctgac
    17881 gataaccata gtgaagataa tggctacacc gaaaagatct ttatctccag gagctgctaa
    17941 tacatattcc gtgatgaatg aaataattcc taatgcgatc aacactaatg taaatggcgt
    18001 gatgtatgct ttcaatattt ctacaatgaa tggtgttttt ttgccatatg aaatcgcatt
    18061 ttcaccgtaa ttttctcttg aacgttcgat ttgttcttct gtcaatccat ttgctgatgc
    18121 atggaatagt tcaaataatt cttgtttcgt ttttccagca aaagctgcat agattttttg
    18181 tgtatctttc tttttttcca tctcaatctc tcctttgtgt ccaaaagaca gctgttctcg
    18241 ctgcttttgg tctttagcta ttcatcggat cgatagagaa aaaactgaaa ggggctgaga
    18301 acagatagat aatagccctg gcctttcctc tagatccttg tttttcgaat gactgtcatc
    18361 catttgttct catctccttt actaagtaat tttaattcat gacttccaat catttttcga
    18421 atagttattt ttccaattag accacctcct ttttattttt caccgatggg gatttgcggc
    18481 aacaaaaaaa cctctcgcca aatactcagc gaaaggtttg aaaaagcata ttaaaaaaag
    18541 cttctcagat ctcatcgttg agttttgaca ctgtacaacg taaaaagcag atacctgctt
    18601 ttagctacct tatgaaaagc cttatctcga caattcctgt tctacccatt ggcgtctctc
    18661 gacgtttttg ggcagtagcc tatgtttgta taggagcctc acctaacaaa tcttattcgg
    18721 ttttccttcg ttaatataac aactaaaaaa gaggatgtca aatcaatttt ccatgttttt
    18781 catacttttt ttaaaataca cacgttatat tcctgtctca cgcggtttta tttgtctcat
    18841 tttccacctc ccgctataat agaaatatag taggaaacga ggtgctgtta tggaaagaac
    18901 acttgttatt atcaagccag acggcgtgag acgccattta gtcggaagca tcatccaacg
    18961 ttttgaagca aagggactgg ccattgctga aatgaagttt gaaactatga ctcctgaatt
    19021 agcaaaagaa cattatcagc acttgactga gcgcagtttt tttgatgaac tgatcgatta
    19081 catgacttcc ggtcctgtcg tttatcttgt gctggtcggc gaggaagtga tcgatatcgt
    19141 ccgtaagatg gttggagcaa caaaagctgc cgatgctgtt ccaggtacga tccgaggaga
    19201 ctacgcgctg ccggggactg aaaacatcat tcacgcttct gattcccgtg atgctgcggt
    19261 aaaagagatc gcccgctttt tcccgccatt cgatataaac aaaccgtctg aaaaagcggc
    19321 gaagtgatac aaattctaaa ctgctggtct agttgctggg ttcagcaatg gccagcagtt
    19381 ttttctctaa ggacctccgt tgattctttc ctaacatata ttcgagcact gtgaagaatt
    19441 cttggtagtc tttcgtcttt tcttccccta aagcagcaga aatcaatctt tgataataat
    19501 ctgccatcaa tgtttcatag atcactgtac ttctttcttt atgtgataag ctccgtttca
    19561 tatcttcatt cacactacgt gcctttttca accgaccatt ttctatataa aacgaagtca
    19621 ggttcattaa aaaagtaaag agatactgct catgattgtt caactgtgct aatcgtcgca
    19681 ttcttttttt gattttcaaa taattgttct caatgaaccg atcactgaat atccaaggaa
    19741 aatcagctag aatagatagt tcaaattgac catagtctgg tatctgcatc aagtattctt
    19801 caatgattcg gcattcataa cgtaaaaatt caaattctgt ctcagatact tctgtttttt
    19861 cgaccatgtg aagattcaaa accaaataat acagatcttc tgttttctta aaatgcctac
    19921 ggttattgct gatctcttta taaggatcaa agctgtcttc ttgtacccga tctagcattc
    19981 gtcttttttt ctttaacttt attggttcat ccagctggtt tcgcatccat tcatattctg
    20041 caagtgtcat gttcattcgc tctaaaaatg atctcaatgt tacaaatgac atttttttca
    20101 ctgaatttgt ctcaatcccg actagagaag tcttaaaaca gattccttcc gctaattggg
    20161 actgtgtcaa cttcttgttg actcgaagtt tcttgatcaa ttcaccatca tacataatag
    20221 ttctcctttt taatttaaat ttttccacat tcaataaata gatacgttaa aaaggaaaaa
    20281 tacacgaaaa aaataaaaaa atgttttttt cataaaaaat accaaccttc aagcattaga
    20341 atgttgtcta actactgaag gttggtttga ttgtttattg attatttttt tgctgttcaa
    20401 cgtctgttct tgttactgga tataagttgg ttgttgtttt attaccattt tttgaaaaga
    20461 cacttgtcga atgttcgcct ttttgctttt cgattgcttc attacgttcc gcttcatttt
    20521 tatagtcata ttttttcgga tcaatcgtct caaatccgtt tggcacataa aaccgcaaga
    20581 gattttcctg attcagctta tctgaaagac ttaacgcagt cttgactttt tcctgatctt
    20641 tggagatttc ctcatcgatt cctgctgctt tcgtatcgat gatttctcct gtctgattat
    20701 tgtaagcttt tcctcctagg acggtgtatt tgggtgtcac aaagttccca tttcgaaaag
    20761 caactacttg atcatgctga gaagataata agtctgtacc aaaatgaaga taatctttat
    20821 catcgattcc caatagatgt agtaatgtcg gcaaaacatc gatttctcca ccgtattcat
    20881 gattgactgt ccctttagta taaccaggca tatggatcat caatggcact cgttgcatct
    20941 gtgcgttgtc aaattcatcc catgtatctg gatctttacc aagagcgctt gctaaatctt
    21001 tattatctgt attcgagatc ccaaaatgat ccccgtaaat cacaaagatt gaattttggt
    21061 aaataccaga ttttttcaaa tacgtaaaaa attgttccaa tgattgatcc agataatgtg
    21121 ccgttcgaac ataattatct actgtgctat tgccagtatt caaagatgga aagtcgaaat
    21181 tcttgtcatc cgtataataa ggcgtgtggt tggttacgga aagaaatttc gtgtaaaacg
    21241 gctgttgtag atgctcaaga tattgggcag attctctgaa taaatcttta tctaatatcc
    21301 cataacctaa cgtttcgtcg gattcatcaa aataagatcg atcaaagaaa ttgtcataac
    21361 ctaagttttt atatacatga tctctattcc aaaaactagc tacattccca tggaaaacag
    21421 cgcttgtata gccttgcttt tgtccaagga tcgctggtgc ggcttgaaag acattgtctg
    21481 acccaagttc tgtgaataat gagccttgag gtaaaccaaa tgtgccagtt tccaacatat
    21541 tttcagcatc acttgtcttt ccctgtccta cttcatgaaa gaaattgtca aagctgatcg
    21601 ttgcttgatt ctggaaaata ctatttaaaa acggtgtgac ttcttgtccg tctaccttca
    21661 tattaatcaa aaactgctgg aaactttcta aatggagaac gatcacattt ttccctttag
    21721 cgatcccaaa tgtttcggga tttggctctg cataatgttt ctttgtataa tctaaaactt
    21781 catcaatccc attactgctt gcatgcgcgc gaacactgct agtcattcct gttttgattc
    21841 cgtcataaat agtatacgca tcgattccta gatatttgac aatatatgaa cggtcaaatg
    21901 ttcgctgcag caattgtgga cggttggctt cgctcagcgc tagatttcct gaaaaaataa
    21961 gccctgaaag acaggtaaca gcgatagcca tcggtttgcc gacagggttg cttttgatag
    22021 gtattttctt gaccttcaac caaaccagta gcccaatgaa cacagcgata tccagccagt
    22081 agatgacatc atggggcttc atcaaagaaa aggagcttcc tgacaatcct tgcgacactt
    22141 tcgaaaagcc taatacagat ttgactgtaa tgaaatctgt gaattcccga taaaaaataa
    22201 tattcaagta caacaaaagc gtatttaaaa tatcaatgat caaaagaata gctaacgctg
    22261 gctttggctt tttaatatac aaagaaaggc caaaaaacaa taaggtagtc gcaattgggt
    22321 tgatccacaa gataaagtac tggatcgtac cttccacacc taatgaaaaa tctgcatagt
    22381 aggcaatgat cgtcttcagc cagaaaaaga aaacagctgt caaaacgatt gctaaacgat
    22441 tttggcacaa ttttttaagt tgtgtcacgt atatcacttc tctctattca cattttttct
    22501 tattttgctc ctgaaaaatg aaaatttcct aaaaaagttg ttatcttaag atttttttat
    22561 tctttctaaa gattattccg tccacttttc acagcataaa accgctgcga tgtaagcgat
    22621 taaactaatg aagagataaa agtcttgtgg tgaccgcttc actccacggt attcaaaagc
    22681 cagtttctcg gtattcgtac aaaaaaagag gctgtgacaa aattcttgtc gcagctcctt
    22741 atttcttgat gcaggacagc tttttcacaa cttcttcctt aaacggtgtt ctcctgccaa
    22801 ctcctcggcc acaagtcaca atatttataa aacatgagaa gctgtttttt gaaattgttc
    22861 cttgtggctc agagttaaac ggcaggctca agacctcttg atacacggtg ttcaatcagc
    22921 tgaccctcgg tattagatta tgtcaggacg ctaattttac aacctcttgg taaacggtgt
    22981 tcaaaagctt gcatctgcgg taataagccc aaccaaacga aaaatataag gagctatttt
    23041 ttgaaattgc tccttgtggc tcagagttaa acggcaggct caagacctct tgatacacgg
    23101 tgttcaatca gctgaccttc ggtattagct caaaaaacgg caaaatatga ggaactattt
    23161 tgccgtttgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc tgtttttcat
    23221 tgtccatagg attgatattg tccttgtgat gactcattcg taataattga ggcttgattt
    23281 tcttcacttc ccgttatcgt ttgtccactg ccagaatctg aaggacttgt tcctgtcgac
    23341 tcgttttttt gagaagagac agttgttttc ttgacttgtt catttgtctt tttccattcc
    23401 gctaaacgat cggcgaccac ttttttgtct actttttcag aataagcggt gatcactttt
    23461 ccatttttca aacgggctac tttgtcgcca tcatgagtca aaagcaaaac ggtcaaatgc
    23521 ttgtctttaa attgatattg tctgagagaa atcgcactaa gtacgtcatc gatattaata
    23581 aattgatttt cttcttttcc ttttggcaaa taaatgtcca attcgccttt ttcgtaattg
    23641 gcagttattt tatactccgc ccaccaataa gttgagaatt cttttttgaa attatctact
    23701 gattctttgc ttgctacgat tttttctgtt tctttctgag caagatctgc tgtcattttt
    23761 cctgaaaatg ctgcggataa aataagcagt aagaaaaaac cgaataacca aaagagaaga
    23821 aacggtcttt ttttcctcat gctttcctcc actcctattt aattttgact attctattat
    23881 atacaaaaaa tgaaatagaa agacaaaaaa gttgaaaaag atcgtaaata attgaacttc
    23941 tttttcacaa aatggtaaga atggagaatt tcacgcattt gctctcaact attctttctt
    24001 aagcctcaaa tccgttttta tctgatacgt ctttgaacca atgacccgat tttttgattg
    24061 ttctttttcc atcttgtgct aagtctactg aaataaagcc atatcgattc ttatatgcat
    24121 tggtccacga ccagttatcc atacatgtcc acatatgata gcctaaacaa ttggcacctt
    24181 cttgaatggc ttgatggaca tatttcagat gagattggac aaattcgatc cgataatcat
    24241 cgtggatcat gccatcttct ttgataaatc gtgcttcgtt ttctacaccc ataccgtttt
    24301 cagaaataaa gcagtcgata tttccataat gatctcgtac attgatcaaa atatcataga
    24361 ttccttgttc atagatctcc catccgcgat aaggattcat tttacgacca ggcatttcat
    24421 aattgtcgaa ataatcctca ggcattggac cgtttttagt atcgattggt gtttcttttg
    24481 cttttacccg gcgtggctgg taataattga tacctaaaag atctactgta ttttctttga
    24541 taaccgtcaa atcctctgca tggatcacag gcatgagatc aagttctttt actatctcaa
    24601 tcaattcact tggaaactcg ccttttacag ctggatcaag gaatgaacga ttgaagaacg
    24661 catctgctat ttgcgctgct ttaacatctt cagggtttga ttcgtctctt ggatagcttg
    24721 gtgtcagatt caagatgata ccaataacac caccttgctc catttcatgg taagatttga
    24781 tggctaacgc tgaagccaac gcttcatgat agccaacttg gatcgctttt ttcatatctg
    24841 actcttctgg ataatgccat cgatataaat aacctgcttc gactggaacg attggttcat
    24901 tatgagtaaa ccattttttt actttcgaac caaacaattc aaaacaactt ttggcatatt
    24961 tttcataggc ttcaaccgtt tctcggttca accatccgcc tttttcttgc agatacatcg
    25021 gcatatcgaa atgatacaga ttcacaaagg gttcgatacc gttttcaatc aactcatcaa
    25081 aataacgatg atagaaacga acggcttctt cgttgattgt tccagattca tcaggtatca
    25141 aacggctcca ttggatagat gttcgaaatg aattatgccc agtctgtttc atcaactgta
    25201 catcctcttg atacttcgta taaacttgcg aggttttttc aggtcctact tgatcaaaga
    25261 acttttgcgg ttcttttttg taccaataat caaaaatgtt ctctcctttt ccatcgcctg
    25321 aaatccttcc ttctgtttgt gggccacttg ctgcagaacc ccaccaaaaa tttgctggaa
    25381 attgatattt tttcatcaaa gcacctcttt ttctttttta gttcgtttta gttgattcca
    25441 tgtatgatat tttccatcac aatcactttt tcattttctt cctcttcttt tttgattcgt
    25501 tttaacagct gttctgctgc tcgttccccg attttatctg ttggtggcgt gatcgtgatt
    25561 ttcggaagga tactcagatc gtttttatct aaataatcat agtgactgaa cgcaatatcc
    25621 ttcccgattc tgacaccttc ttgattgaaa cattcgatgg caccaatcga catattgtaa
    25681 ttgctgacaa aaatagcagt tgcccctttt tctaataatg cttttgcccc gtaatagccg
    25741 ctttttctag aatagtctcc tgtaaagacc tctaattgga tcgatggatc attttggacc
    25801 gcttctttgg ctcctgctaa acgttctctt gctacgtagt ctgtttgagt ggcagcaata
    25861 aatccgattt ttttatgtcc ttgttcacca tgatgagcga tcacttttgc tacactttct
    25921 cgatcattca ctaatattgt atcaatacct gctggtcctt ctggacaatt caatgcgata
    25981 actggaatac tcatttttgt gagcatttca aatccagacc actcttcttc ggataagaag
    26041 acaatcagcc catcgatcac atgcttcatc aggtattcaa ttttttgttc aattttttct
    26101 ggctgatcgg caaacccgct taataataag ctatacccat tttcttcagc tactctttcg
    26161 atccctgaga caacttccga cccaaaacgg ctggaaatcc cgtttatcaa aagtccaata
    26221 gagaagcttc gattattctt caacccttta gcaatgatgt tttctttata gtccaacgct
    26281 gaaatcgctt ctgctatttt tttcatgttc ttttccttca gttgctgacc attcaagtaa
    26341 cgggaaactg ttcccactga aacaccagcc atcttagcca catcacggat cgatgccata
    26401 cattctcact cctgccagtt acatttgatt tctctttctt gttgttcgat tatgatgacc
    26461 gcattctttg catagtgttg cttgtcatca atatccatat aactaaagtc ttcagtagag
    26521 caacttcctc tcactattaa ttatacttaa acaagtattt gaaccggttc atttttaata
    26581 tagcattttt ttaaaagatt gcaacacttt tttaaaacgc tttctgaaag tggcggattt
    26641 tttgcctttt taaaatttta ttctaaaatt tactctattt gtgggtgttt tgtgcgtttt
    26701 tctcttccat ccgtttgaag ttttacattc caatggtata atttctttat cctatcaaat
    26761 gggggaagta ttttgaagaa attaatcgta ctatttattt tgtctgcggg acttttgaca
    26821 ttgactggct gtgatttgtt tggcggctct gaccaatcct cggacgaaac aaccgattct
    26881 tctgtcttaa aagcggaaaa agcagatacc accaacagtg tatatacgct aaaagtacgt
    26941 aaagatatgg atttatccgc gggacagttg acatttacag ataaaaaaat ggaatggaca
    27001 cgcacgtatc agacacaagg aactgattct tctgaatcat ctgaagaaaa aacagtgctg
    27061 acagatatca agatcactac caaagatgac gaatatatta tttcaggaaa agaaaataaa
    27121 aaagaaacaa cagtcatatt tacaaaaatc ggtaattacc gtttgaagga tgaggatggc
    27181 aacatctaca gtctatagtg ccgtctgttg gatcttcttt tgtttctccc cttcttttct
    27241 gctatagtaa agaagcaaga tgattgtaaa ggagacgaaa tggatgatcg atagtcctga
    27301 ttattgtaca tgtaataaca acaaaaatat ccatacagaa tttaatgacc aaggaagctt
    27361 gagcatatgc gacaactgtc acaaactagt caaagattca tttaaaacat ttgaatctta
    27421 tcctgttcaa aattatgtaa aaagtagaag aagataaaca aatccaagca aaactcacta
    27481 tggctgattt ttatgaactt caaactcagc ttcttccttt gatcaagctt catcagtaaa
    27541 acaaacagtg gatcatttca gtttacaaat caaagagtaa agatttattt atttcaaata
    27601 tttgaatata aaataaccaa acgatagaaa ggcttattct acatcacgta gtaatttttc
    27661 tttcccgtcg tttggttttt tatttttagc ctaattcttg accttttgca tgctggttat
    27721 ccgtaaaata ttttccagca ggctttcctt ccaattcagc taatttttct tggatctgac
    27781 ggatcacctt ttttgcatct tctggatttt ctacaaaatc gaatttatcc ccatccactt
    27841 gtattttagg acatacatca aaatcttcat accattgatt atagcgggtg ttcaacgttt
    27901 cataataggt atataacgtc tcatcgcttt ctagctgttc atagtcacgc ccacgttttt
    27961 tgatacgttc tagcatcgtg tccaaagaaa ctttgatatg gaccatcaaa tcaggccgtt
    28021 tttttggagc aacatcatct aactcgttta gcatcgtatc taataagcta tcgtactgct
    28081 gtacttcgat atccgttacc cgacctaaat cagcgtttag atggaacaac aggctatctt
    28141 catagatgga tcggtctagc acattgtcat cgttcactaa agcatttttc attgctaaaa
    28201 atcgcttgtt caaaaagaag atttgcaata aaaaagcgta ctgctcagga tttgaataaa
    28261 agagcggcaa tacttcgtta tcttcgacat tttcataaaa tggtttagaa tcaatctctt
    28321 ttgcaagcat atccgtcaaa ctgctcttac ctgcaccaat cgttcctgaa atcaaaatca
    28381 ctgacataca acccttcctc ttctataagt actcgcctac gtaagtttca acaggcaatg
    28441 tttcaaaatc atattcacgt acagcttggt ctgattttaa atacgtatgg agactttgtg
    28501 taatcatcaa gttcgtaggt accgtctctt cttgtacaat gatcatcggt tttttcaaca
    28561 tagttgccac acctaattcg taagccgttc ctggatcgat tgtttgatgc tcaaagtcaa
    28621 ggatagcaac aacaaattct gcatttgtca gttcttccat atctttttcg tatacttcct
    28681 gcgcccatcc agcgctgaat agttcataat tgctttcttg gtgatgacgt ggagagtaga
    28741 aacttgtgac tgttttgttt tcctccaacg ctttttcgat acgacttact cgatcgattt
    28801 gctcctctga aaaaaatggt cctgctaaat atatttgact cattattttg cctccttcaa
    28861 taataactga tttttaatag atactgctcg taattcattg accagttcca atggattgtt
    28921 ggaaaagatt acttgatcca accatttttt atccataaaa tcctcttttg caaaagtttc
    28981 taataattgt tgcaagccgc tgtaataacc gccaatattc aatattccga ttggcttgtc
    29041 gggcgtcaga ccgattgcta tccaagaaag aagttgtgag aattcctcca atgtacctag
    29101 acctccaggg aaaacgagga cagcatctcc cttttcaaat aataatacct ttctttcatc
    29161 gatcgtttct gtaggaataa atgtagtaac atcttttcca ggcaattcag tttcaaaaag
    29221 cccattcgga tagatacctg tgacttttcc gttattttcc agtactgttt tactaatcac
    29281 tcccataatc ccggagtatg aaccaccgta aaccagttcg atttcttctt gtgccatgta
    29341 ttttcctaac gtttgcgcaa taaatttgta actttcttta ttaccaaaac gggaaccgca
    29401 aaaaacagta agtttcataa aaaaacactc cctaactcaa aagtctatct tcattctaca
    29461 cgtgtttttt taaaataaaa gactagattt tgtagttttt tagtggtata taggatagac
    29521 gccactatat atagtattta gaaaaaaatc atttttcagc cattttatag ataaaacaaa
    29581 aataaataac gtgttttcat cgatatgaaa tgacttactt atagaagata ctgggatttc
    29641 gtcctatatg ccaataacgt gtattatttt aaacaacaaa tacttgtttc ttttattaac
    29701 gaaaaaatca ttttgttctc gtgttatgtt tcactgataa atgatgtttc tctaaataaa
    29761 aaagccgacg aactaaacga tcaaaatttg atcatttttg ttcatcagct tctcctaaaa
    29821 atctttttgt taacgataaa tagctggatt tgtaaaaata tcacttgaat cgtcactgct
    29881 ggaagaaaat tcagtgataa ccgcacctgt tgcgcctgcc tggaactagt gtttgatatt
    29941 gggctcgaac gtgtactgat ctctagcatg aagaataatt tcttttttta ctgtataata
    30001 actttctttc ctttttggaa tagtacagga aagttttttt gtttcctttc cttcgacata
    30061 aggtatacca gaccctttcg acaacgaaag gttctcattt ttccttcatc gtcatttcaa
    30121 ggagggtgcc gatgctctgg acagtttcga tcgctcctaa accaaaatct gtatattcga
    30181 ttctttctaa ctctgattct ttcataccat tcctgtctca aggtacatct attgtacttt
    30241 tttcttgtaa tccattctgc ctctcctaac taattgtcaa atcatcccaa gatatgtctt
    30301 cttcctcttt ttcttcttgg atgatttctg cattcggatc tactggtttt ttcaataata
    30361 aaatggttat tcctgtgaca aaagaaccag ctaacaatgc accaatgaat gcccatggtc
    30421 tggtcatcgt agcgactgca aacattcccg atgccggaat acggctatct gcgccccaaa
    30481 acatgcttaa tccgccacct actgtcccac cgactcccgt tgctatgaca ttgcgtgcca
    30541 agtcgttcaa tacgataggg aaacatccct ctgtgatctg acagatcccc ataggaaaag
    30601 cggtcttcaa tgtatcgatt tcttgacgat tgagtatatt tttacgaatg attttcccta
    30661 agaaataagc gatcgtcaag ccgaatgggg ttaccatcga agctaaaatc aatacagtga
    30721 ttggttcata gattccttct gaaaatcgtg ccacaccccc ttatccagaa taaatggtaa
    30781 tccagaaaca gccgtgtatc tgcggtaata agcctaacca aacgaaaaat atgagaagct
    30841 atttttgttt ggctgttctt attacttgat gcagggcagc tttttcacaa cctctttttt
    30901 tatttatgtt tcataatcaa tatggtaagg atgcagctcc gtatggtaga ccgcctccga
    30961 atattcaatc atttccccaa tttgcgtaga cgatttccgt atccgtttga tgccgatctg
    31021 tgcgtctgtt ttcagaattc cttgttcttc ttctgttaaa aatactgccg agaaatcatc
    31081 agaaaatgag tcaatagtat atcctttttt cgtcagcaaa cgatacaaag attctttgcc
    31141 aaattcttct ctgctgaatt gcatcatttc ttgtggaaga taatgaacaa aataaatata
    31201 aggctgattg ttcaaataat ataagcgctt aaaacaaaca gcttgctcac cgaaaaagga
    31261 aaacaaagga tcttccttct ttatatggac caattgcaaa tccagcgttt ctttccggac
    31321 ttcatatcgg gagttctcca agatttgcgt aaagcttgcc gctttggata atcgattgta
    31381 aggacgattg cttagaaccg tcgtcccacg accacttttc tttgcgacat attcatctga
    31441 tgccagcagt tcgattgcct ttcggacagt gattttgctg acttgaaaca gttcttctaa
    31501 ttcactttct gtcggcaaca tcgaaccgac tggatatttt cctgagaaaa tatcttcttt
    31561 aattttatct gcgacttcca agtatagtac acgtgtcctt ttcataacgt caccctttgc
    31621 tttactatta ctaaaagaat agcacataaa aaataaaaaa agaacacaaa tcacatgtga
    31681 gaagtgtgtt ctttttcatt cagctgcttt aattacaagt ttacgccatt tgaggcaatt
    31741 acttctttat accaatcaaa agattttttc tttgatcgat tcagtgtccc atttccttca
    31801 tcgtctagat ccacatagat aaagccgtaa cgtttactca tttctcctgt tgatgcactg
    31861 acaagatcga tacatcccca aggcgtatag cccatcaaat ctacaccgtc tgctactgca
    31921 ttagccatcg cttcgatatg acgacgtaaa taatcgatcc gataatcatc aataatcgtg
    31981 ccatcctctt ccatatgatc aatggctcca agaccgtttt caacaatgaa taatggtttc
    32041 tggtaacgat tgtataattc attcaatgcg attctcagcc cttctggatc gatctgccag
    32101 ccccattcac ttgcctcaag aaacggattt tttactcctc ccagcaagtt cccttctgaa
    32161 gttgctgctt gtggatcagt ctcattgatt gccgtagaca tataataact aaatccgatg
    32221 tagtccactg gatattcttt tagcaactgg aagtcttttt ccgtttgctc taatttttcc
    32281 ggatcgactc catatttttt atgggtacgc gctgtgtatg cagggtattc tccacgtact
    32341 tggacatccg tgcagaaaaa attgaattct tggttttgga tcattgttgc tgcctgattg
    32401 actggattgg catcgatgct gtaagttgtt gcatagataa tcatgcagcc gatttggata
    32461 tccgaacgca attcgtggcc gatttttacg gctttgctac ttgcaacgaa ttgattatgc
    32521 catgcttgga aaatattttg gtactctcct gctccattgc tttttactaa tccttgactt
    32581 aaagctggaa agtgaaaggc cgaattgatt tcattgaaag tcatccaata tttgacttta
    32641 ctgctgtatc tctctaatac tgtctgagca aatcgttcat agaagtcgat cagttttcga
    32701 tttttccagc caccgtattc ttttgccaaa tgaagaggca tttcgtaatg agaaatcgtg
    32761 atcacaggtt caatatcata ttttaagcac tcatcaatca gctgatcata aaattctaat
    32821 cccgcttcgt taggagtacc ttcgtctcca tttggaaaaa ttcgtgtcca ggcaatcgaa
    32881 aaacgataac atttgaatcc catttccgca aacaaggcaa gatcttcttt gaaacgatcg
    32941 taatgatcga ttcctcgatg attcgggtaa atatattttt cttgatcaat cgtccagtca
    33001 aattcttcac tgccgataat cgcaaagcgc tgcttacctc caggcatagc atctgcaacg
    33061 gatagtcctt ttccgtctcg atcataagcc ccttctaatt gatttggcag cagttgctcc
    33121 accccataaa aatttttctg aaaaacgtga ttgcatcctg atttcctcct tattttacaa
    33181 ttgccagaac atcttcgttt gcaataatct ctttttggtc taattcaagt atatcggcaa
    33241 atgcatcagt attcgtaatg acaactggag tgattacgct atagccagct gctctaattg
    33301 cttcgatgtc aaaacgaatc aataaatctc ctgctttgac gagatcccct tgtttgacta
    33361 agatctcaaa gccattccca tttaattcta ctgtatccat cccgatatga atcaaaatct
    33421 ccgctccgtc agtcgttgtg atccctacag catgtccagt tggaaaaata gtcgtgatct
    33481 ctccgtctgc aggtgcaact agttctccta cttctggatc aatagcgata ccttttccca
    33541 tcacgccaga agaaaaaact tggtctggta cttcactcaa tggcagtact ttacccgtta
    33601 aaggactttt taaggtatgc cgtgctgaag taatcggttc tcctgcattc gcatgtttgt
    33661 tttctaattg attgtcttgt gtttgttcgt caaatcctaa aatcaatgta cctacgaatg
    33721 caagaacaaa agcaattcct gtagctatta ctgctactgt cacatttgac tctacaccat
    33781 ccaccgtatt aatgaaagtc ggaatcgtca ataagctgac caaactgctg gagaaagctt
    33841 ttactccgga aaatccgata atagctccac caatacctcc agaaatacaa gcggcaatga
    33901 atggtttctt caaaggtaat gtcacaccat acacagttgg ttcagtgatt ccaaataacg
    33961 aagtcactgt agaagaaatc cctaatgctc gaagtttcgt atcttttgtt cgcaatgcta
    34021 cagctaaagc agccccacct tgtgccaaga tggctggaag aagcattggc atcaatacat
    34081 caaacccata ttgttcgata ttcaaaaaca tgatcggtac gaatccccaa tgcatgccga
    34141 acatcacgaa tacttgccat aaacttccca tgattagacc tgctacgatt ggactaaatc
    34201 cataaatcga gttgaagaga ccgcctaaag cattaccggc aaccgtcccg attggtccta
    34261 aagcaagaaa tgttaatgga accatcacta ctaaaacgac aagcggaact aaaatcattt
    34321 gcagaaattg cggaatcact ttttttacga atggctctaa tttactttga acccatacgg
    34381 ctagaatgat tggtatgaca gaagaagtat aaccagatgg tgaaagaatc actgggatcc
    34441 caaagaaact gatagcttct cccgcaccag caactgctgt gatagctgga tacacaagtg
    34501 ccatagcaat tgctactgct aaaaactggt tggctttgaa ctttttagct gctgtaaaag
    34561 caagcatgac aggtaagaag gtaaagacac catctgctgc agcaaacaat atttgataag
    34621 taccgctatc agctgtcagc cagcccatca ccgttgccaa tgataagaat cctttcaata
    34681 ccccggcggc tgccatcgcc cctagaaaag gtgtaaatat actagagata atatcgatca
    34741 gacgattcaa aatatttttg gactcttctt ttccatcctc cgaaactgca agaccttcct
    34801 catccacgat cgattgataa acatctgcta cattactgcc aatcactact tgatactgac
    34861 cgccactttg tacgacttgg atcacgtccg ggtcttcttt taacttttgc gtatctgcta
    34921 aagattcatc ttttaatctg aatcgcaacc gcgtagcaca atgtactaaa cttttgacat
    34981 tcttttgtcc tcctaccgct tcccatacac gacgaccaac tgcttgatta tccattgttt
    35041 tttccttctt tcttttttat ttaaaaaata aaaacccaga ttgaaaaaaa cagtcacaaa
    35101 cattcgtgaa cctatttttt caatccaggt tttgcctgcc atacagtaac aatccttatg
    35161 attggatgct ttttttctca cttactactc tttgaatatg aatcgttaaa taaatctgct
    35221 ctgcgatccc catttcctgt ttttgcgttt gtcgaagata ctcattgatc cgttcgacac
    35281 aatgaaacgc ttttggatac tttccttgaa ctaatgcgta gagaaattca tcttgctcgt
    35341 cagatccttc ccctttgaga taacgctggg caaaaaactg cagatgagta atgatacgct
    35401 gataattcaa agattcttca tcgaacacga tgccgaagaa tttactgatg atactcagta
    35461 tatctcttac cattgtggta gcggtcatcg tcacttgcat gtcttcgttt ccttgttggc
    35521 tattcacaaa atgaaaggca ataaaaccgg cttcatcctc agaaagttcc acacccgtct
    35581 tttgtttcac gaacaaaaga gcctctttcg ctgcttgata ttctttcgga taaaattttt
    35641 ttgtttcaaa taataatgga ttaggcagtt ggatattttc ttttgctcgt gtgatcgcat
    35701 aatgaagatg atcggtcaat gtcagataaa tattaggcga aaattcaact tttagtttat
    35761 cttcagccag ttcgactact tgtttgaccc attcgatctc ctctgccgga atatcagcca
    35821 tcagctgctg gaattggttt ttagcaaaag aatctcttaa acaaattctt tttggaccgc
    35881 ttctggcgaa atctcgtccc caatcttttt ttgaaaagct agtccttttc ccatacaaac
    35941 gacttcttcc ccgaatccat ttttcgaaat gacgacattg ttattcaaaa ttttatcaat
    36001 tttcattttg cttcgccctc tcaagaaaaa taaaaaaacc tagaattcgc ctactgtttc
    36061 ttagctgaat tctggttttg cctgccgtac agtaacaatc catttcttat tcaaaatcat
    36121 cataccatcg aatttttatt tatgcaagcc ttttcatata aaaaataaaa aaactggaac
    36181 acaagtctat ctagaaaaca accaaatcag agatgcacct ctttgcgttt ctcttttttt
    36241 cagcaaattg gtttttcctt atcgattcat atcctttggc tgtttttaat ctgtaacgac
    36301 ttatgtccca gtatcctaaa atgtttttgt ctctagattg aaagcagatc tcatatcatc
    36361 tgtcgcatct tttatcgatg taacactttc cagttctcct tgtacaacta gtcgcgtgac
    36421 ggcgtccatg tcgccacaag taattagtgt gacaatttct tttccctcga cttcattcag
    36481 taattgggta tctgtaggag caactttttc tttaaaaaat gttttgtatg tatacacatt
    36541 ttctaaatct gtcaaataga tcttggcacc catctctaaa ttttctagcg gtgtaaacag
    36601 caattcaggg ttcgttgcac gatggcttgc taaggcataa tttccttttc ccatttcttg
    36661 atctggagat aatgttcctg ctccgtaaag tagtgacgta ttatccaagc ctttgaaaac
    36721 cggtaaattg attgaaactg aagggactgc gatactagca atcactggta gatctgttcc
    36781 tctcatttgc gacctcatga ccccttctgt cgtcattggt tctaccgcat caaagtcaaa
    36841 actgacatct ttatcgttat tctttttcag atcttctttt gttacattcg ctatagcata
    36901 tttatctcct gtctctctga caaaataatc cttgatctgt tcattgaaaa tcaaagctag
    36961 tcctactaat aacaagagga acaagaggat attgactagc catctttttg cttttccttt
    37021 ttttttcatt ttctctccct ccagactcat tttactgttt ttcttttgct ctttccaaca
    37081 gccgaatgac acatttctac aactctgtgt atttttttcg tacacctgcg cttttttcta
    37141 caggatattt caaattattt ttgatcaatt tttcttcaat gatctcatct agatccaggt
    37201 gaaggttgtc tgccatcatg tatgaataga tcaatacatc tgccagttct tctttgattc
    37261 gttctaattg tgtttctttt acttcttccg gcgttttcca ttgaaaaagt tctaacaatt
    37321 cagatgcttc caaagaaata gagatagcca aatctttttc gttatgaaat tgtcgccaat
    37381 tacgttcatc tctaaactgg ttgacttttt ccatactatt catttgcttg ccttcttcca
    37441 atcacttctt acttgattaa tcataccatt tttttacata aaaaatagat gatgctttta
    37501 aaaaaactcc tgttttcttt tatttaaaat aaatgtcata gaaaatcaat aatttaggta
    37561 gccaccatgt gtatgcagta gcgtgtcttc ttcccttgag gctttttgtt tttcgatacg
    37621 ttcttcttcc caaacagaca acgctactag aatctctctt aatgattttc cttcttcggt
    37681 gagcgagtac tctactttag gcgggacttg gttataggct tttcgatgga tactttcatc
    37741 ttgttctaac tctcttaact gctgggtcag catttttttt gtgatcgtag ggatttgctt
    37801 gcttctccac gcctaaaata agcgatattg ctcttccctt tatctggtgt aagcaaagca
    37861 gttagcattt tgacgatcgt gggttccgct tttcacttct tatctactta gtatcatgaa
    37921 gtatactttc gtactaatac taagtagcgg aagtgtcaac tactttgttt aactacatag
    37981 caaaagaaac agaagggtga caacggtcat cacgcttctg tttcttttga atatgctgtg
    38041 ttgttgtctc tatctccgtg tagactttcc tcaaacttat cctttgacag taagtcagta
    38101 ggctgatttg ctctttctag atgccagata catttctaca aaaaaatagt gattctgttt
    38161 tgaaaattcg atttttagtt ctgcattttc gtaaacatcc aacatttttt tcgcactgta
    38221 tagacctaac ccgccatttt ttttagaggt ttttcctctt ttgaccatct ccgatagcgt
    38281 caaattcgtt ggatttgcct tgttcgctat actaaaacgt gatccctctt tttcttccag
    38341 cagattgatc gtaatcggac tctgatcggc tacacgatga tcgaatgcgt tagaaataaa
    38401 aatagataaa cagcgaataa aaacaaccag atcaatcggg attgttttca cttcgacagg
    38461 aataactact tggatcggga ttttctccga ttctgctttt tctagaaatt ttaaaaagac
    38521 acccctgaca gctggggata caatatttgt tagctgttgt atttgcggtt tttcaaaaat
    38581 agctgcagaa tgatcttcta atatttctaa aaattccaac gtttcttttt tattttcctg
    38641 aagagtaatt tttaatgaaa acagtagact tttataatca tgtttgaatt cctctagatg
    38701 atggtaatat tcttcattta tttgatacag ttgaagcgat tcctgatgtt gtctttcttt
    38761 tacttggaaa ctgatcaaca taatggaaaa aaacaaagaa aaaaaactga tgactatcga
    38821 caaaagagat agaagcagat aattagatga gcggaagtga acacttccca ataccaagta
    38881 aatcacgccc cagcttaata aaatacaaat acctccaaaa tagtgttcag ttggataagt
    38941 aaaaagcaag ttgagaaatc tcgtctgttt gtctatactt tttactaata gatagaaaat
    39001 aaatgcgata agtagtgcaa aaaaacttgc tgttccaaga tgtctctttc ctaaataatc
    39061 catcagatca aaaaccagaa aaaagctgag tccagtgata gcaaaaaaaa gattactgat
    39121 caaacagctg ataaacaggt ttttcgtagt aaaataaaaa agaaacatca ataataagcc
    39181 aactaggtaa ggaaagctca ctttagggat aggaagtaaa agtgtcacca ccatcaaagc
    39241 tatacaacca aagctgtttt ttactagcag ttgtttcgaa aagaataaat agatacctaa
    39301 atctaaaatc aaagtaaagg tcaaataaag tgtcactgtt gcgaccatct tattcctcca
    39361 ttgttttgtt tcaaaatgta cacatatttt tttaatagga gcctgcatat gaaaaagtat
    39421 tttatcatag aagataacca gtttcacagc gaatggatca tcagagaatt ggtcaatcat
    39481 catatcgaga atatcgtgca gctcacttct atctctcaaa tcaatcagtt gatccagttg
    39541 ggcactattg ataatcacga tgttttcttt atcgatatcg acttgaagca atcactcaca
    39601 ggtattgatg tcgccgaaca aatacgtaaa aaaaatgcac atagtaatat tgtttttttc
    39661 accgcttata cgaattttgc agtagagatc atcaatcgga aaatccttcc gattggatat
    39721 atcagaaaag acaggaattt atctgaacaa attcgtcaag tgctagaaca gatcaattgg
    39781 aaagaacagc agatcgtaaa tgtcgataaa atctttattg atcaaaaagg tggaagtctc
    39841 gtcctgtatc ctcgggaaat tttatatatt gaaagtatca aaggtttgaa aaaccaagtt
    39901 atggtcaaaa caactacaga agataaatta gtgaatactt ctttaaaaca attgaagaca
    39961 gagttagaaa agtatgctta ctttttactt ttaaaatctt actgtatcaa cttatcgcaa
    40021 ttgcagaaaa ttgattctgc tcattatgtg ttagaatttt tcaacggtga ctccttactc
    40081 gttggtcgaa agatctttga aaagacaaag gaacgtttcc atgacttcca aaaaactgct
    40141 tcgagttgat gaataagagc tgagacgtaa gcatagcaga tttcagggag tgggccttga
    40201 atcggctcca ctccctgaaa tcgttgatat gattttacgc ttcctcttct gtaggagcat
    40261 ctgttaacga ccatgtgaat gttgaaacgt agttgtctac agcataattg gatacaggaa
    40321 caaataattc cactgattgt ctcccttgtt ccacattctc tcctaatgaa taaatccatg
    40381 tgccaatccc ttcatttgct cctgcagtta ctagatctac tgcctgatct gctgggattt
    40441 cgattccttc agcgccagtt ccagaaacgg tagatggcat tccactgcta ttagttgcgc
    40501 tatttcgaat cacggcgttt ttgaatttca cctttgaacc agttagttta ttcgtatcag
    40561 atgttgtacc aataaattct gttcgtttga ctgataattt ccaaccggca taatttccgc
    40621 ttttatccgt tacttgtacg aaattctgta cttgtgtttg tgctccagta ctgtccgttc
    40681 cgctttgtaa tgcagcatat actgtttttg ctgtacctaa cttgattgtt ccaaaatcaa
    40741 gatctggagc ttggtcaatc gtcagtaatc cggatgttcc ctgatttggt tcgtcaggtg
    40801 taaaaggagt atcaggatca tttggatcaa caggaggaac tggaacaacg tcttcaggat
    40861 ttgcagcatc tggtttggtc acagaaatcg ttccttctgt agtggctgag ctcttattct
    40921 cagcaaaaac actttgtgta cataataata gacttccagc tagaacggta cttaatgcaa
    40981 ttgttttttt catttctttt tccttctttc ttttaaaata aataatccag caaataagcc
    41041 aataagaatg actccaacaa gtatcagcca tcgccagtca aatgtttctg gctgattttt
    41101 taaatcaata tccttttcat tcaattggtc cgctgcatca tcagttattt caaaatcggt
    41161 atggagattc cagcgttttt ctttccaagc acccttcaca accgcttgat aatgtcccgg
    41221 agctaatttt tttccttcta atgggacaag taaatcaaaa tttgtattgg gtgctacatc
    41281 tattttttct ttttttattg aaaggtaagg ctcttttgtt tcttttttgt agatttcaac
    41341 cgatgtagtc aagccgaaca tataagaagc atttggattt tgaagattcg ccttaatcac
    41401 atttcttcca tcgctttgat ccgcataagc tttttttaat ttcaaatctg gggaagactc
    41461 tttttcgcta tttcaaatca agattgcttt cacttgagaa aactgattga cgacaccaac
    41521 tgtacttttg ttagaaacgt tctctttttt ttcttgaaac gtgatccctc ctgcgaccac
    41581 accagccagc tctttttctg gcgctttcag tgaaagtgtc tcagtgaccg cactttgagg
    41641 aggaacagtt atttctccca ctgtttttgc taagtttttc atatcataac ttaatgtttc
    41701 atcaaattta ctttttgatt ctccatattc gatcacaccg tttgaattcg tcgtagcgga
    41761 gtggatcaat gtctcaactg ttactgattt atctgttcgg ttaaagaccg tgtaggggat
    41821 ttcctgtgtt ttcccaggct ccatccttac atttatatat gttttgttct tatcaatctg
    41881 attagccggt aattcaaaat ctaccgcaaa attgaattcg gaggcatgga caggtgtctg
    41941 ccaaaggatg aatccaagga gaaggatcat aagtaaagag cagttgtttc gatgactttt
    42001 attccatatt cgattcatca atcatcctcc ttgatcttta ttctgtcggc gcatctgcta
    42061 atgtccatgt tatctctgct gagtaattac cagctatctg attttctttg gttaagacaa
    42121 gtttaaacgc ccgaccgtta tttaaatatt ctgaataatc tgcttgcaca ttcgttgaat
    42181 tttgtcctct gacaatttcc agttcttctt cattcactaa ttgatactcc tctcctttct
    42241 tgaaatatag gacatcctgc atagaaaatc ctgcatcatt tgtgaaaggc tgagataatt
    42301 ttatcgttaa gatccacgga tttttcgttt ctcgctgatc ataaatccat aatttttcct
    42361 gtatatccat ttggccaaat gcttcttgtg tcaaaccagt tgctttgatc tgaccaaaat
    42421 cgagtttgga aggggcaaga aatgagagac caccttctac tacgatcttc gtagcaggtg
    42481 caaaaggcgg ggcatcatga taaacaactg gcgtatcact tggattgtga ttcccaattt
    42541 catcgttggt acttggcttg ttctgcacac ccttagcagt agcttctcct gcttggtcgt
    42601 ttaatacaat ttctaataca tctcctgcaa aaagttgatt ttctgggata gaaagaacaa
    42661 attcccctgt atcatcaata gttgtttctg acaaaacagc ttgatttttt ttcaatgaaa
    42721 ccaaggctcc tgcttcacct gtacctgtga tcgtgcgaga agctgcattg atcaatgtgg
    42781 tacttaaatt agcaggttca ggaggaatca catcgactgt cgttttggca gagtccagtt
    42841 ctgtcacttc ttggccacct cttacttttt tagcagcaac aacttgcact tgatcctccg
    42901 tttttaatct ctcatcattg ggtacttgaa cttcgaacaa gccgccttgt tcgccttctc
    42961 cccatgggtt atatcctgcc ccttcatttt catctgtttg tccttgcgtg agagcagtaa
    43021 tttctacaga tgatttcgtc gtatctgcgt aagtaatacg taaagtcact tctacttcgc
    43081 ctgtccaagc atctcttggc tttgcctcga caccttcggg aacaacgaca tgcccataga
    43141 tttttttatc tgcattcgtt ggcactctta aggattcaat catcgggagc tgattattgg
    43201 cactgattcg attataattt ttcattccgg cacctgtctg agcaaattca tttgttaaac
    43261 ctgcactagg gcttgatacg aaattgccta agtcggtccc attgatcgat aagtctgtca
    43321 tatcagaaaa ataatccggg tctttttcaa agttatcgaa tgttgcgcct gtttttcgcc
    43381 ataaagcgat ttctgaatgg gcgatcttta aagaagaatc tccatttcct tggaataccc
    43441 agccgcctat gccattggtc tcgcttccaa tccgtttgtt ttggaaatca aaataggtag
    43501 gagattctaa gtccacattc aacacatccc cactgatgat gccggtctta tcttcccctg
    43561 ttttcccgac catgactaaa taagtttttt cacctgccgt aaaatttaaa tcaatatctc
    43621 cagttgtatc tacagttgct ccggaatctg cgcgtatatc gatatttgat ttttctccag
    43681 tgactgtaaa atcagctgaa cctggacttt ctagaaaacc agaagcgtca taaaattgga
    43741 tcgcttgatt tccttgattg agtctgttca cgtaagattt gccatcacct gtatttctta
    43801 ccctcaatga gcctccattg gttactgtca gcttttgcgt accattctca aaacgaactg
    43861 cgctggaacg cccactctgc ttatcgatgt cgataactga tccggctcct tctacgatca
    43921 aagaaaggcc ttttcctaca aaacgcatcc cattgttccc gggttgatcg tcatctccag
    43981 ccattttgac cgagatttgt ccaccacttg atacatggat ttgactagaa tctccttgca
    44041 agcgaaatcc gcccgtagtt gctgtattga tggtgatttt cccaccattc gatacagtga
    44101 ctttcgatcc tgaacctctc atttggatga ttccgcgatt gttgtcattg gtaatattga
    44161 tctttgtatt taccgaaaat tcgcttcccg aaccactgac ttcaaacatg acattaccag
    44221 gtagcgtatc gactgcctct ccttcatcgc taacgccatc tgcccgatca taatcaagag
    44281 agacctttga accgcttaag atatggattc ctggcgctgt ttttccacta ccagtctcat
    44341 ctcctgaatc tagaaaaccg tagaatttcc ctagatacgg tccataaagt tgactaccat
    44401 tgatcacatt caattgatag cttctacaaa aataggcttt acccggagcg gcattcagtc
    44461 cttccttcac tggttggaca tccaacacac cggaaacacc atctaatgtg acagttgcac
    44521 gaggagcgta aatcagtcct aatttggacc catcatacaa atcaagtgtc ccggtaaatt
    44581 ttatttctcc cgtaaaatga tatctgcttc cccaaaaatc ggtcatataa gcaatgataa
    44641 tggcatttcc atcgatattt ggtgtcaatc cacctggatg tgcagctgtt cctttaaaat
    44701 tctggaactc gatcgactga ggtatctcat tgatataaat atggtgtgtg ccgaaattgt
    44761 gagcgaatcc accaaaatca ataacgatat ctcgtttgat ctctaattcc ctgtctaaag
    44821 tcaaaggctg aatcagtcgg atcttagtta ctgattcgtc tgcgagaagc tgcgccagtt
    44881 cttctgttgt ggcagcttcg ctgtacccat ccgctgaaac agctcttgcc agtgccgctt
    44941 cactgctatc agacgtttgt tcagctactt ctgaggtctc acttgtctta ttttcttctt
    45001 gccttttatt agtagaagcc tcactttgag attctgtagt ttcagaagta atcgattctt
    45061 ctgaaaaagt agtcttgtca aaactttgat tctctgattg cgttgattcg tttgttttct
    45121 ctacatcggc aaaaacggat gtcaccatca agagagggaa gacaaacaaa atcgacgcaa
    45181 tcagcttttt tacacattga ttcattctcc accatcctct ctgacgtttt gacaatttta
    45241 ttttatcgtt ttaatctatt tccaattgta ctttcatatt taaaatatgt atttttatgg
    45301 aaaaattttt ctattaggtg taaatttctt ttttgtacaa aaaaaaggct gtgacaaaag
    45361 tcttgtcaca gtcccttatc ccgaacaagc ggtgttctcc cgccaactcc tcggtcacaa
    45421 cctccatttt aacaataaac ctgtaaatgt gagggcaata ctttgattgt cacaggtaat
    45481 ttatcccctt catctccatc aacattcgtc gtcaattctt cctcatcagc taatgacaca
    45541 actaattgct cacaagtaag gtaggctaga tttttcgtag attcatctac tccttttaat
    45601 aggtctggca atgttttaag cgtatcgatc aaagaagaat cttttaaata aaccaaatgg
    45661 agcttaccat catccacttt tgcatttgga agtaatgttt caaaaccgcc gactgagtta
    45721 gttagtccta tcagcaaggt actgctctca atttcttcct tctttccatc aacttctacg
    45781 tgaaacgagt aggattgtgt ggaagctaac tgtttgattc ctgacatgaa ataagctagt
    45841 tttccaaatt tcgtcttctt ctccgcatct acatcattga ttgattcagg gatagagcca
    45901 attgcgacga cattcataaa atagtcgtca tttatttttc caatatccaa tggcttgact
    45961 gattcaatcg aaaaatgatt gatcgcttcc tcaggttcta gcggtatccc taatgctcga
    46021 gccaagtcat tcaccgtacc caatgggaaa aatccgaaat ttggccgatg ttcttgttca
    46081 gcgattccac tgattccttc gttgactgtt ccatctccgc ccatgacaaa cacactatga
    46141 tacccttccg tagccgcttc gcgagtaaag ttttttgcat cccctgcttt ttttgtatgc
    46201 aagacgacca cttcatcaaa caccgattcc aattttgcta tcgctaattg ctcaaattcc
    46261 tttgcttgtt caccgcctga actggggttt accactaata aaactttttt catcactttc
    46321 tttcccttct gattgatttc tttaatataa cgatagcata aagaaaaaaa ttaggctgtg
    46381 atgaagtact gttcatgaaa cattcattta ttttttgaca agataaaaca tccatactat
    46441 gaaactgagg aaaattaaaa caatgatata ggaaataaaa gaaaatgata ggcattgtat
    46501 acctaataaa gtaaaaatct taggtggcag ataaaaacac aaaaatagca agagaatgta
    46561 atagcgtttc ttccactttt tgttatcaga ccttatagcg atcagaggaa taccaaatgc
    46621 ataactgaaa aactcttgat gtactgttaa tatgtcacgg ccttgaataa taaatgcgac
    46681 aatccccgct ataattagtg tgcctaacac caaaacggaa aattgatgaa tatattttat
    46741 atacaaatga tccattgtac ttcctgcctt tttatttgtg agaaaaacaa atccgtatac
    46801 gtgaataact tctagaaata aaagaaattt gcaaaaccac tccatttatc tacttctacg
    46861 tgcatttttt gatattgttt tctttattta agattataca gaaaaatatc tagggtcgct
    46921 ggattctaag taaaaaaaat ttttaaaata aaaagaacta aatcaagtag taattaaact
    46981 actgttgatt tagttctaat aaaaagattt agatgaacag ttttattgat ttaaatagtt
    47041 catatttttt atttataccg cttatttact acttgctgta ctagaatctg aagtgaatgt
    47101 actcaaaaca ttttcaaagt ctttgtcttt gatttttaca ttagcgtctt tcaatacttc
    47161 gccaattact ttattctgga aggtactgtc acttaatttt gtgtctgttg caatttcttt
    47221 caattctttt ttgtatttat ccatgtcgtt gcctttgttt tggtttttca ccattttaac
    47281 cacataatat tccgtagtat aagttgaagc attggtagaa gtgatcacat cagaaacttg
    47341 accatctttc aacttaaagg ctgcttcttt gacttctgcc ggaaccgttg tagtagtgga
    47401 atcaaatttc acttttccgc catcttcttt tgtcgtagta tcagttgact tgtctttggc
    47461 taatttagag aaatcgtctc ccttatcagc agctttcttc acatcttttg catcatcttc
    47521 acttgctact tggataattt gagcttccac ttctggatgg aaagattccc aagccgtttt
    47581 taaatcttca tcggtcaatt tgatatgttt ctttagacct tcttgaaagg ctagactttg
    47641 tttgatactg tctttgaatg tttgttctgt gtaacctgca gatttcaatt gtgaatcaaa
    47701 tgaatcaccc aattgtttct ttgtttgatc atattgagaa tcgatttctt tggttgatac
    47761 gtcatctcca tatttatctt caaatacttg ataaacgatc aactgttgca cagcggaatc
    47821 tgctccttgc atttttttca agctatccat tacttcttgt tcgctgattg taccgccttt
    47881 ataactaacg gctgtttttt caccggcatt cgtacagcct gacaataagc cagtgcctag
    47941 tacgatggca agtgctaaaa atcttttttt catttatcaa ttctcctact tttcgttttt
    48001 tgcgaattgc taaaaacaaa ggtgttcaag atgaaaatca tcaagaacac ctttactaat
    48061 ctgctcgata cgtcctgaat agatataagg ctacacaatc cagaaagttc gctcagcaga
    48121 ataaatgtca agtagcacat cacaccttaa tatactactt cttttgttac cgtttttcaa
    48181 gtctatcttg cgttccgtat ttaacgtaac taattcttag aacaaatgtt atcgattttg
    48241 tttttcttct tccgctgctg ggatattcaa atcagacact tttatgttcg attttccaga
    48301 catttttttg ttgtttttat tatgatatat ttttatacaa ctaaaaatga atatattaaa
    48361 actaccaaaa ataactttta acgctgtttc attatatgtt ttttctattt tatataaact
    48421 tttggatact taataccaac cggttggatt gtaccaacat gagaatgtgt cagttctatt
    48481 tcctgtcgca cttgcatagg cccatactcc ttttgttgca tttgctgatc gaatagagtt
    48541 tccaactttc gcttttgcac cgtgacgatt atagacacga ctatagtggt tagaatgacc
    48601 tgtttttctc gtacaagttg tatcactata ataaacccat cttccagagc tatgattaat
    48661 tgctgttcta gaaatttccc ggtctactgg taaatttgac ttaataatat tacctttagc
    48721 atcaaaatga atctctcctg gctttaaaga tttgtaatag atttcaccgt gaactgaatt
    48781 tgcttccact tcagatgaca tagcgtttcc tccaacaaaa cctatcccta gtgcacaaat
    48841 aataactgct tttttcataa aacgacatcc tttccttttt gatttttaaa gcatgatctt
    48901 cttgtttcct attttggtca gatatggatc atgcgtgact acgataacta acgtaccttg
    48961 ggaagcttgt tgcgataaga tttcaactac tttatcctta tttcttttat ccaatgaccc
    49021 agtcggttca tcggctaaaa tgattttgct attcttcaat aagactctcg cacaggcgat
    49081 tctttgcttc tcaccaccag ataatgtgta gataggcgta cttagcttct tgtttatatt
    49141 tacttttttc aatgcctcat actttgctgt tcgcttatct gaaacattta aattcgaaaa
    49201 cttcaatcca atatctaaat taaaatcgat tgtttcatat tcaatcaacc caaaatcttg
    49261 aaaaagaaaa gatatggtat ttctttttat caagcgcttc tctctcccag ttaattgttt
    49321 cgtctcttga tcgaacaaat agaattgtcc tgaactgaac tgatcattca atcctaatat
    49381 attcaataac gtacttttac ctgctccact ttctccaaca acagtcacaa gatccccagc
    49441 atccgcagtg aaacgatcca aatgaagaat cgttttgttt cgaatgattt ttttcgcacc
    49501 ttttatcact atcaacggct ccaattgtta acctccattc atcagcaata actgactttt
    49561 cttttctgta gacaaaataa aaacaactga aagaataaaa tcaatgaagc taaagaaaaa
    49621 aatattaaaa agatatttag gctctctttc tataagaaaa aggaagatta cacttgtcgt
    49681 tccccatttg atgaggttca tcatgaagaa atctttatac gtttcaaaga gcgaataacc
    49741 acttgaacga aagatagcga ttttccgaca attgtagaca aaatatatgc ataacatgac
    49801 atagctcaac aagaagaaag aaaagaaagt gagtaaaaat atctgtatca agtaatgaaa
    49861 tgatctgctt aacactttga cagctatctc ttcagtctga gtatacggaa atgataactg
    49921 caagttatca cgtaactgac tttcttcaat cagctcaaat aacctttctt tcgacaaaga
    49981 gcctatcttg atctttaacg gtggatagat ttctccgctg agaatatttc tttccaaata
    50041 catactgttt ttcaatgtca gtatttcaac aaccggataa tgttcgatcc aggggataga
    50101 aggaataaat gtataaatgg gttgattgtt ttttataaat agaaatttcg gttctttttc
    50161 actgatttct tgataatatt ctgttatttg cgccaggtca ttttttaaat ctgtatgtct
    50221 atatcgttca gggatcaata taacagggtg cgtttctttt tcggttattt ggatcaacct
    50281 tccagagaca tcaatcaaag gaaatttctt aagataattc agatttattt ttatccctct
    50341 gccaaactgt tcattttctt cattcaagta atctgataca ttgactaata tacttccgtt
    50401 tttattcaaa gtttcatata attcctgatc tgctgctgat gcaaaacgat tggaataaat
    50461 aaaatccacg tggtttttcc cgatggttac tggataaaat acatgatagt cctcaccagc
    50521 gatttctttt ggtggttcat gggaaacagc taactgtgaa gaagaaaaaa cgatagttgc
    50581 taattcgaca ttggcaacgg tcaaaagaag agaaaacaac aatccaaatg taaaaagaat
    50641 gtattggata tagttgaacg cttctcttaa cgaagcgatt ttttcaacta ataaaagaga
    50701 aatcaaagag ataccatata ttccgctgaa caagaaaacg tacatctgga acaaataaag
    50761 aaattgctcc ttataataca taaaaataaa accaccaatg attcctgcaa ttatgaatgt
    50821 aataatgatt ttctttttcc ccaataaaca ccacaatact tgatgtaatg aaaagtcatt
    50881 attcaacaat aaactgattt ctttattcat caataagaaa aaggatgata acaaaaggaa
    50941 taaaagaatc aatccaatag ttaaatacac tcgataatta tatacgtctg tatttgaaat
    51001 aaccgaatac gaatagctat tttcaaagtc ttcaaaaata ttagtggcat aatcagtgtg
    51061 aaatgcttga ttgaatcgat cagtgaatag tgaaatgaac cgttcatagg cttctttttc
    51121 tctcgtcatc aagaaaaatt caccttcaaa atcttttatt ttgatcgatt cttcaatgga
    51181 cgttacgcga aaggaggtat tagaataggg taccttctcg tgagaagatt tgacatcccc
    51241 ttgataatac aatgtcattt caagctttgg ctgtatcttg aagttccaag atgtatctac
    51301 ttcattggat tcatttactg tttttttcat ataagaaagc tcagtttctg atgagacatt
    51361 ttcaaatacc atgattagct gctcatattc ttcttgccca ataaaatgtt gtggcaatcg
    51421 aatcggagta taagtatctg tttgatggaa aaattcattt gtccgataga gtacctgttt
    51481 atttatacta accaaggcaa aaccactcat gataccggtc actatgattg tgagtaaaac
    51541 tgtgaacttt tttttgatta tcaccactct cccttactta aatgattctt tgataaaatc
    51601 ataccgtttt tcatcaaaaa aacgtatcta tcaagtgtat aggtcatgta gaataaatct
    51661 atatactgta gaatcgaaag gagaagaaga tgaacaccgg agaaaaaata cgaacttacc
    51721 gcagaaattg taagcttacg caaaaagaat taggcgaaaa aattggtgtt tccgataaaa
    51781 cgatttctag ttgggaaaat tctcgtacaa tgccagacct tgaaatgctg tcacttttac
    51841 atcatacttt aggtctgccg attgattttc cttctattga atcagctacc gaatcaatag
    51901 aaccaacatt gccagatgaa cctcttgtta acaagaaatc ttattcatca aaacaaccct
    51961 tctcactcaa aaagacacta tctttctttc tgctcgtctt ttgtttggtc tatctttttc
    52021 ttatcaatta tcaatacaaa aatgaaacga tcgatcgatt caatcctttt ttgaaaccac
    52081 aaatcggcta taccacactc cctgtaggat acgtggacaa ttccatcgaa cgttgggtat
    52141 gtaatgatat ctttggccat ggtacatggc tgtcttttca cgctggagag gtagaaaaag
    52201 agaaaaacca tgctgtaatc aaacacaaag gaaaacacgt catgaacgtt cgcctgctca
    52261 cttgggaagc cattccctca gattaccaag aaatcctcag ccaagactat gacgacgcct
    52321 taaaaagaag ccctcaggag ccagataggc attaagtagg agatgaacgt agagatagaa
    52381 aaaatcaatg cacatgatct tatccaaaaa acaggggtac aaattaattg aaaaagcctt
    52441 gagatgaaag ttcctaaata aaaaagttca gaatgatagg tatttctggc ttatagtata
    52501 atatttacgc cacagcctcc caatgattct ttttggaatc ataatcaagg atggacattc
    52561 acaaaaatat tttgctcatt cgatccgaat agattaacga ccaatctagt aagtaatata
    52621 gattggtacg tagtcaagct gcacataatt tagatcaatt ttagttcttt tttatatttc
    52681 tatgatctgc tcatttactt ttatatgatg gcttacagta acataaataa atatgataat
    52741 cgctggtagt ttatccaaga gtactcaata aaatacaaaa tggattgttc aagcgcctat
    52801 cataatgata aaatgaaaca aataaacgat acaaggagga agagactttg agtaatggca
    52861 gaacaatttt ttcattgtaa aaattgttca gttgttaatc tatgggattt cggtagcatg
    52921 ggcatttttt tatagaggaa tcacaccaac ctggccgttt tatctcttgt tagtgggaat
    52981 attagtagga aattttgccc ctcttcacta tcgtacgaac ttttttccaa ctgattctgg
    53041 cctttttctt aaaacaaaga cgcgaaaatt agaaaacatt attgaaatga caattacctg
    53101 tataatcatt ttcttagtta tattcctaaa cgtcttctaa taagaatctc ttaaaattag
    53161 cttgttacag ttagttacct agtttatata acacagttat cttatcttcc ttatcaacat
    53221 cttttgccaa ctctttgtct taaattcgaa acatataagc agagctacga acaccaagac
    53281 tgtttcggct ctgttttttg catcaaattc actcttatat agctgataat ctcggctctt
    53341 taaaagaaat tattttcgct tcattttatt ataaaaaggc taggaacctt gatgttatca
    53401 ggttcctagc ctttatttaa gtattacccc acagaccctt ccatatagat ttttaaatta
    53461 cacactagtt taaactaaaa caaacaagga aacacttacc atttaatcat aaaattagct
    53521 catatgaata tattagaatg taaaatagac tggtcaaaat ctggtcaaaa aagaggacta
    53581 atacaggtag t
//
LOCUS       DADVXU010000009        50891 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.009, whole genome shotgun sequence.
ACCESSION   DADVXU010000009 DADVXU010000000
VERSION     DADVXU010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 50891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.009"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            147..518
                     /locus_tag="KB953_000519"
     CDS             147..518
                     /locus_tag="KB953_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HBB6865518.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(792..1484)
                     /locus_tag="KB953_000520"
     CDS             complement(792..1484)
                     /locus_tag="KB953_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBB6865519.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(1487..2272)
                     /locus_tag="KB953_000521"
     CDS             complement(1487..2272)
                     /locus_tag="KB953_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBB6865520.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            2509..3909
                     /gene="lacG"
                     /locus_tag="KB953_000522"
     CDS             2509..3909
                     /gene="lacG"
                     /locus_tag="KB953_000522"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HBB6865521.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            3934..4251
                     /locus_tag="KB953_000523"
     CDS             3934..4251
                     /locus_tag="KB953_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBB6865522.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            4264..5937
                     /locus_tag="KB953_000524"
     CDS             4264..5937
                     /locus_tag="KB953_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HBB6865523.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSGMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            5951..7582
                     /locus_tag="KB953_000525"
     CDS             5951..7582
                     /locus_tag="KB953_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HBB6865524.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(8073..9638)
                     /gene="guaA"
                     /locus_tag="KB953_000526"
     CDS             complement(8073..9638)
                     /gene="guaA"
                     /locus_tag="KB953_000526"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBB6865525.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(9697..10620)
                     /locus_tag="KB953_000527"
     CDS             complement(9697..10620)
                     /locus_tag="KB953_000527"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HBB6865526.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            10816..11421
                     /locus_tag="KB953_000528"
     CDS             10816..11421
                     /locus_tag="KB953_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBB6865527.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            11729..12745
                     /locus_tag="KB953_000529"
     CDS             11729..12745
                     /locus_tag="KB953_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBB6865528.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            12886..14187
                     /locus_tag="KB953_000530"
     CDS             12886..14187
                     /locus_tag="KB953_000530"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HBB6865529.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            14233..14895
                     /gene="deoC"
                     /locus_tag="KB953_000531"
     CDS             14233..14895
                     /gene="deoC"
                     /locus_tag="KB953_000531"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBB6865530.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            14916..15314
                     /locus_tag="KB953_000532"
     CDS             14916..15314
                     /locus_tag="KB953_000532"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBB6865531.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            15380..16462
                     /locus_tag="KB953_000533"
     CDS             15380..16462
                     /locus_tag="KB953_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBB6865532.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            16674..18236
                     /locus_tag="KB953_000534"
     CDS             16674..18236
                     /locus_tag="KB953_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6865533.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            18229..19362
                     /locus_tag="KB953_000535"
     CDS             18229..19362
                     /locus_tag="KB953_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6865534.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            19362..20318
                     /locus_tag="KB953_000536"
     CDS             19362..20318
                     /locus_tag="KB953_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6865535.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            20417..21583
                     /gene="deoB"
                     /locus_tag="KB953_000537"
     CDS             20417..21583
                     /gene="deoB"
                     /locus_tag="KB953_000537"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBB6865536.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            21607..22425
                     /locus_tag="KB953_000538"
     CDS             21607..22425
                     /locus_tag="KB953_000538"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBB6865537.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            22442..23149
                     /gene="deoD"
                     /locus_tag="KB953_000539"
     CDS             22442..23149
                     /gene="deoD"
                     /locus_tag="KB953_000539"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBB6865538.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(23447..24133)
                     /gene="gpmA"
                     /locus_tag="KB953_000540"
     CDS             complement(23447..24133)
                     /gene="gpmA"
                     /locus_tag="KB953_000540"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBB6865539.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(24305..24886)
                     /locus_tag="KB953_000541"
     CDS             complement(24305..24886)
                     /locus_tag="KB953_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBB6865540.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(25019..25702)
                     /gene="rpiA"
                     /locus_tag="KB953_000542"
     CDS             complement(25019..25702)
                     /gene="rpiA"
                     /locus_tag="KB953_000542"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HBB6865541.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            26021..26434
                     /gene="rpsL"
                     /locus_tag="KB953_000543"
     CDS             26021..26434
                     /gene="rpsL"
                     /locus_tag="KB953_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBB6865542.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            26518..26988
                     /gene="rpsG"
                     /locus_tag="KB953_000544"
     CDS             26518..26988
                     /gene="rpsG"
                     /locus_tag="KB953_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBB6865543.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            27082..29169
                     /gene="fusA"
                     /locus_tag="KB953_000545"
     CDS             27082..29169
                     /gene="fusA"
                     /locus_tag="KB953_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBB6865544.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            29341..30528
                     /gene="tuf"
                     /locus_tag="KB953_000546"
     CDS             29341..30528
                     /gene="tuf"
                     /locus_tag="KB953_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBB6865545.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            30935..31243
                     /gene="rpsJ"
                     /locus_tag="KB953_000547"
     CDS             30935..31243
                     /gene="rpsJ"
                     /locus_tag="KB953_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBB6865546.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            31274..31903
                     /gene="rplC"
                     /locus_tag="KB953_000548"
     CDS             31274..31903
                     /gene="rplC"
                     /locus_tag="KB953_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBB6865547.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            31931..32554
                     /gene="rplD"
                     /locus_tag="KB953_000549"
     CDS             31931..32554
                     /gene="rplD"
                     /locus_tag="KB953_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBB6865548.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            32554..32844
                     /gene="rplW"
                     /locus_tag="KB953_000550"
     CDS             32554..32844
                     /gene="rplW"
                     /locus_tag="KB953_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBB6865549.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            32883..33716
                     /gene="rplB"
                     /locus_tag="KB953_000551"
     CDS             32883..33716
                     /gene="rplB"
                     /locus_tag="KB953_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBB6865550.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            33759..34037
                     /gene="rpsS"
                     /locus_tag="KB953_000552"
     CDS             33759..34037
                     /gene="rpsS"
                     /locus_tag="KB953_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBB6865551.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            34060..34407
                     /gene="rplV"
                     /locus_tag="KB953_000553"
     CDS             34060..34407
                     /gene="rplV"
                     /locus_tag="KB953_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBB6865552.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            34421..35077
                     /gene="rpsC"
                     /locus_tag="KB953_000554"
     CDS             34421..35077
                     /gene="rpsC"
                     /locus_tag="KB953_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBB6865553.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            35080..35514
                     /gene="rplP"
                     /locus_tag="KB953_000555"
     CDS             35080..35514
                     /gene="rplP"
                     /locus_tag="KB953_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBB6865554.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            35504..35692
                     /gene="rpmC"
                     /locus_tag="KB953_000556"
     CDS             35504..35692
                     /gene="rpmC"
                     /locus_tag="KB953_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBB6865555.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            35717..35983
                     /gene="rpsQ"
                     /locus_tag="KB953_000557"
     CDS             35717..35983
                     /gene="rpsQ"
                     /locus_tag="KB953_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBB6865556.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            36041..36409
                     /gene="rplN"
                     /locus_tag="KB953_000558"
     CDS             36041..36409
                     /gene="rplN"
                     /locus_tag="KB953_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBB6865557.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            36446..36754
                     /gene="rplX"
                     /locus_tag="KB953_000559"
     CDS             36446..36754
                     /gene="rplX"
                     /locus_tag="KB953_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBB6865558.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            36782..37321
                     /gene="rplE"
                     /locus_tag="KB953_000560"
     CDS             36782..37321
                     /gene="rplE"
                     /locus_tag="KB953_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBB6865559.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            37340..37525
                     /locus_tag="KB953_000561"
     CDS             37340..37525
                     /locus_tag="KB953_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HBB6865560.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            37561..37959
                     /gene="rpsH"
                     /locus_tag="KB953_000562"
     CDS             37561..37959
                     /gene="rpsH"
                     /locus_tag="KB953_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBB6865561.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            37991..38527
                     /gene="rplF"
                     /locus_tag="KB953_000563"
     CDS             37991..38527
                     /gene="rplF"
                     /locus_tag="KB953_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBB6865562.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            38688..39044
                     /gene="rplR"
                     /locus_tag="KB953_000564"
     CDS             38688..39044
                     /gene="rplR"
                     /locus_tag="KB953_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBB6865563.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            39065..39565
                     /gene="rpsE"
                     /locus_tag="KB953_000565"
     CDS             39065..39565
                     /gene="rpsE"
                     /locus_tag="KB953_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBB6865564.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            39580..39759
                     /gene="rpmD"
                     /locus_tag="KB953_000566"
     CDS             39580..39759
                     /gene="rpmD"
                     /locus_tag="KB953_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBB6865565.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            39803..40243
                     /gene="rplO"
                     /locus_tag="KB953_000567"
     CDS             39803..40243
                     /gene="rplO"
                     /locus_tag="KB953_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBB6865566.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            40243..41538
                     /gene="secY"
                     /locus_tag="KB953_000568"
     CDS             40243..41538
                     /gene="secY"
                     /locus_tag="KB953_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBB6865567.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            41600..42247
                     /locus_tag="KB953_000569"
     CDS             41600..42247
                     /locus_tag="KB953_000569"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBB6865568.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            42438..42656
                     /gene="infA"
                     /locus_tag="KB953_000570"
     CDS             42438..42656
                     /gene="infA"
                     /locus_tag="KB953_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBB6865569.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            42689..42805
                     /gene="rpmJ"
                     /locus_tag="KB953_000571"
     CDS             42689..42805
                     /gene="rpmJ"
                     /locus_tag="KB953_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBB6865570.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            42823..43188
                     /gene="rpsM"
                     /locus_tag="KB953_000572"
     CDS             42823..43188
                     /gene="rpsM"
                     /locus_tag="KB953_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBB6865571.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            43216..43605
                     /gene="rpsK"
                     /locus_tag="KB953_000573"
     CDS             43216..43605
                     /gene="rpsK"
                     /locus_tag="KB953_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBB6865572.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            43685..44623
                     /locus_tag="KB953_000574"
     CDS             43685..44623
                     /locus_tag="KB953_000574"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBB6865573.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            44651..45031
                     /gene="rplQ"
                     /locus_tag="KB953_000575"
     CDS             44651..45031
                     /gene="rplQ"
                     /locus_tag="KB953_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBB6865574.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            45303..46142
                     /locus_tag="KB953_000576"
     CDS             45303..46142
                     /locus_tag="KB953_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBB6865575.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            46118..46987
                     /locus_tag="KB953_000577"
     CDS             46118..46987
                     /locus_tag="KB953_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBB6865576.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            46977..47774
                     /locus_tag="KB953_000578"
     CDS             46977..47774
                     /locus_tag="KB953_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBB6865577.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            47977..48723
                     /gene="truA"
                     /locus_tag="KB953_000579"
     CDS             47977..48723
                     /gene="truA"
                     /locus_tag="KB953_000579"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBB6865578.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            49388..49625
                     /locus_tag="KB953_000580"
                     /pseudo
     CDS             49388..49625
                     /locus_tag="KB953_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(49769..50545)
                     /locus_tag="KB953_000581"
     CDS             complement(49769..50545)
                     /locus_tag="KB953_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBB6865579.1"
                     /translation="MNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
     gene            50668..>50891
                     /locus_tag="KB953_000582"
     CDS             50668..>50891
                     /locus_tag="KB953_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBB6865580.1"
                     /translation="MLDQTDINIIEELSKNSRITMKELGEKVHLTGPAVSARVTKLEE
                     SGVIENYTVKLNMEKLGFSIHAFLIIITQN"
BASE COUNT        16139 a         9266 c        11053 g        14433 t
ORIGIN      
        1 atagaatcaa cactcagaga acgggttggt ttttaataga tttaataagt actgataatg
       61 aaatttctcc tggttgggaa aacgaagttg gttaccctgt agaagaattt tttcaagatt
      121 actttgcgga gtatgccgat aggataatga gagattacgt aacagataaa actcaagaag
      181 tcatcaatca atcccagtca ttaaatgaat tatctgataa ggcgaaagaa gaagcagttg
      241 ccattatttc ttcagggatt agtgactata ttgatatgag tggtgaaatc aattatggcg
      301 ataaagacaa aattgtacgc atgataaaag cttttatcaa aacttttgat gtgaaggcag
      361 atcttaagtt taatgatact tacctagtag gaaaattaat taatatcctt gggacagaga
      421 aagatacgga aacgtttatt tcctctttgt ctatgttgtt tacagataag catttttcaa
      481 ctcagcttgg tggagaggaa ctagtggaaa tcttttaacc attacgccca gcattaataa
      541 aagtatatca aaaaagtact cctgatgatt tttaagattg gtttgagaaa taatgtgaaa
      601 acactatttt taatgttaat tatagataga gatgaacgcg tcatatggtt aaaagaatga
      661 aaaagtcaaa gatacactgc tatatttcac taaggatata gcagtgtatc tttcatgtcg
      721 tttcactggc agttttttat aaaaggaggt gtgcatttca gttgcttttc tttggtagtt
      781 agttgaatcg tttaagaatt aacggatgtg gcagacaggt gctctgtcca aaaaccagta
      841 aaatctttga tggttccatc tttatttagc tgtgcctgtt caaatactgt tttgttttta
      901 ccgaatggat caattgcaat aattgttccg atccccgcac gtttggcagc aagtaatcct
      961 gagtaagcat cttcgataac aagacaatca gccggagcta agctcagttt gtctgccgct
     1021 ttaagaaaaa tatccggttg cggtttccca gggaaagagc cgtcgtcata gacaatcttg
     1081 tcccagtcaa accagcgggc taaatcgaaa atatcaaaat aaaaagcaac attctctttg
     1141 acagtagccg tggcaatcgt taatggaatc tgttggtcag tcagctggtc aagagtcgct
     1201 tcaagccctt ttgtcaactg aagctcgttt tcattttgca agcaaagcat acgataatag
     1261 ctttcttttt cttctgacaa tacggcgatc tcagaggcgg tcaaatgctc tgaaatgaaa
     1321 tgtttcaaaa tctccgtatt cgtccgacca tgaatatttt ttaaaatatc ctcgtctgat
     1381 aaaccatctt ttgtatgatc atgtatcata tgcagccaag cagcttcatg cagatgagag
     1441 tccaaaaaca ttgttccgtt gaaatcaaat ataatccctt tcatagttac gctcctatag
     1501 tggtattttc ggttgaatca gcaatttgca gatcgacata ttgtgcatag tgctcttgaa
     1561 cctcccgcga gatctggcga tccgtaatga gcagatcgaa atcatgcaaa ttataaaaca
     1621 cataaaaatc atctcgattg aattttttat tatcaacgag tagatatttc ttacgggaat
     1681 tatttagagc gatctgctgt gcttctcctt cttcggtact gtaagtagag atcgcttcgt
     1741 tgtgaacgcc atttgaactg ataaatgctt ttgtaaactt tagtcttctt aaattgtcgt
     1801 tggtgatcgg tccgacaaat gcgccggtat ttttccggaa atcaccgcca atcagtagaa
     1861 tttctggtcc atctgcttga gcaagaatat caaaaactgg ataattattt gtaatgatcc
     1921 gaattttctt gttcagtaaa tgcgtcgcca atagttcgat tgttgtacct ggtcctaaga
     1981 aaatcgtttc tccctcgtga atcaaggagg ctgctgtggc agcaatttct tttttttctt
     2041 gaatctgcag agtggctttt tcagtgtgag aaagttcttg atctaaagtg taggaaagac
     2101 tttgagcccc tccatggaca cgcagcagct tgcccgattt ttctaattca tctaaatctc
     2161 tgcgaacggt catatcagaa acatctaatt gattgatgat ttcattaacg gtaaggattc
     2221 cttttttatt gaccatttct acgatagtaa gcaatctttc ttttttcaac attgtcttca
     2281 cctctaattt aaggataatc tatcatataa acggacaaca gtcaatctaa ttcgaacaaa
     2341 agtaaacatt tagggtggtt tttatgtttg ttacaataga ttttattgct gttttctggt
     2401 ttttatgatt atttgtgttt gtttttgttg acaaatgttt tggatgcgtt tacaatgcaa
     2461 tcagttagaa agagcgaaaa ctgattatag atttgaggag agatgtacat gcttaaatta
     2521 ccagaagatt ttatttttgg cggagccaca gcagcctatc aggttgaagg agccactaag
     2581 gaaggtggca aaggcgctgt tgcttgggat gactttttag aggaacaagg gcgattcagt
     2641 cccgatccgg caagtgattt ttatcatcaa tatgcaaagg acattgaatt gtgtgaacgt
     2701 tttggtgtaa atggtctaag attatccatt gcttggagcc gcatttttcc tgatggagct
     2761 gggaaaccaa atccagaagg aatcgctttt tatcatcgtg tgttcgaaga atgtaagaaa
     2821 agaaacgtaa cgccgtttgt gacgttgcat cattttgata cccctaaacg tttgtttgac
     2881 catggagatt ttttgaatcg agaaacaata gaagcatttg tttcctatgc cattttttgt
     2941 tttcatgaat tcaaagaagt caaggtgtgg agtaccttta atgaaatcta tccagttgca
     3001 accaatcaat atttgttagg tgttttccca ccaggaatca aatatgattt tacaaagatc
     3061 gtcgcctgtc ttcataatat gatggttgct catgcccgtg tagtgaacta tttcaaagaa
     3121 aacgaacttc cgggtgaaat tggtgtagtt cactcattag agacgaaata tgcggcaaca
     3181 gacgcgccgg aagataagca tgctgccttt ttagacgatg cattgtcgat ccgtttctta
     3241 ttagatgcta cctatttagg ctattattca actgaaacct tgactgctct tgatgagata
     3301 tgtgaagcaa atcaggccag ctatcatttt cctgaagaag atttcgttga attgaaaaaa
     3361 gcatccacgc gtaatgacta cttagggatc aatcactacc aatgccattt tgtcaaagct
     3421 tacgatggag aaaatgcaat ccatcataac ggtacgggtg aaaaagggac ctctgtttat
     3481 aaagtgaagg gaatcggaga acgtatttat aaagaaggaa ttcctcgaac tgattgggac
     3541 tggctgattt atccagaagg tctatatgac ttgttgctgc gaattaaaag cgattaccca
     3601 cattacaaca aaatctatat cactgagaac gggatggggt ataaagatca gtttgaagat
     3661 ggcattatta tggatcagcc gagaatcgac tatttgagag tctatttgga atccttaagc
     3721 aaggcgataa cagccggggt aaatgtgaag gggtattttt tgtggtcgct tatggatctt
     3781 ttctcatgga caaatgggta taacaaaagg tatggcttgt tttatgttga ttttgaaacc
     3841 cagaagcgtt acccaaaaga atcagcttac tggtacaagt tggtcagtga aaccaaaacg
     3901 attatttaat aagtgataat ggaggaagaa aagatgaata aagaggaatt acaaatgctt
     3961 ggttttgaga tcgttgcata ttcaggcgac gctcgcagta cattattgaa attattgaaa
     4021 gaagtaagac aaggaaactt tgaacatgtt gattcggcgt tgaaagaagc cgacgaaaat
     4081 ttaacccttg cacataattc gcaaacaaaa attttagcgg aagaagcatc tgggaaagag
     4141 atggagatgg gattcatttt tatccatggg caggatcatt taatgaccac tttattgctg
     4201 agagatctga ttcaagactt tattgtactg tacagacaga atcaaggata ggagaagaac
     4261 gctatggatg gaatcattaa acaaatcgaa aaagggaaac cattttttga aaaaatctca
     4321 cgtaatagtt atttaggagc cgttcgtgac ggatttttaa cagcgatgcc agctatttta
     4381 ttttcaagca ttttcatttt gattgctgcg gttcctgaga tttttggtta ccaatggcct
     4441 gaatctgtct ccacatggct atggaaagtc tataattatt cgatgggtgt tgtagggtta
     4501 ctcgtttcag caaccactgc tcgctgcttg gctgattcga tgaatcgtcg cttaccgaaa
     4561 aacaaagtaa tcaacaccac ctctgtgatg ctggcctctg tggtaagttt cctgttgtta
     4621 agcgtctctc cgattgaagg tggattttca acggaatata tggggacaaa gggactactg
     4681 gcttcctttg tcgctgcatt tttaacagtt aatttatata agttttgtgt gctgaaagat
     4741 attaccatta aaatgcctaa agaagttcct ggaacgattt cgcaaacctt ccgagatatt
     4801 tttcctttct ctttttcagt atttgcggcc gttttaattg atttaggcat tcgacaaatc
     4861 ttccaaatct catttgcaga agcaatcatt acggtgctgc agccgctatt tacagcagct
     4921 gacgggtact taggaatcgc tattatctgg ggagcgatgg cgctgttttg gtttgtcggt
     4981 gttcatggac cgtcgatcgt ggagccggca attgcggcaa ttatctatgc caatgtggaa
     5041 accaatctgc aattgttcaa ttccggtgaa caagcttcta aagtactgac ggtgggttta
     5101 gggaattttg taggaactat gggaggaact ggggcaacat tggttgttcc ttttatcttc
     5161 ctactctttg ctaagtctaa gcagttgaag gcagttggga aagcttcctt tattccagta
     5221 agtttcgctg tcaacgaacc attgctattt gcggcaccca tgattttgaa cccttatttt
     5281 tttattccat ttttgttaac acctgttgta aacgtttgcc tgttcaagtt ttttgtcgat
     5341 gtattgggca tgaacagctt tatgtacgtt ttgccttggg caactccagc accgatcggt
     5401 ttggttctag gaacgggcat gggtatctta gcttttgttc tagcagcggt tctaatcgtc
     5461 gttgacttcc tgatctatct accattttgc aaagcatacg atgctgtctt agtagagaaa
     5521 gaagcacaaa aagcagaggt gatcgcaacc gaggaaggtg cttctgccga tgtacgtgag
     5581 gaagtacctg tggcagcgag tcaagttatc agcgatgcaa ataaagaaat caatgtgttg
     5641 gtactttgtg ctggcgcagg gacaagtggg atgttagcta atgctttaaa aaaaggtgca
     5701 tctgaatcga aagcaccgat cagtgcaatg gcgggtgctt atggttctca ttacgaaatg
     5761 ctgcaggatt ttgatatggt ggtcttagca ccgcaagtca attcttacta tgatgatatc
     5821 aaagaagaca ccgatcgctt aggaatcaag ctggttgcga caaaaggagc agaatatatc
     5881 ggattaacga gagatcctga aaaagctgtt cagtttattc tgaaccaatt tcaataaagg
     5941 aggagccata atgaaaatgg gaatttggtt gcgtaaagct gttttatttg cagctgtgtt
     6001 aggggctttg ggggctgttt caaaaactgt tgaagcatct gccagcgaat ttattcgcgg
     6061 agcagatatt tcgatcctgt cagatatgga aaaatctggt gcaaaatatt atgaagacga
     6121 tatttcaaaa gatgccttag agattttaaa aaataatggt gtcaactatg ttcgactgcg
     6181 tttgtggcag gatccttata atagtaatgg agaaagttat ggagcaggga caaatgatct
     6241 ggaaacgacg attgaccttg caaaacgagc aaaaaatctg ggacttaagg tgctgctgga
     6301 ttttcactac agtgattttt gggttgatcc tgggaaacag aatcttccta aagcatggca
     6361 aggtctgacc tttgaagaaa tgaatacggc attgtacgac tatacaaaga acgttctgtc
     6421 ggaaatgaag cagctggacg tttatcctga tatggttcag atcggcaatg aactgaatag
     6481 cggaatgctc tggccttatg gcaagtcttg gggagaaggc gggggagaat ttgatcgatt
     6541 ggctgctttt ttgaagtctg gtatccaagc tgttcgagac actcaaccta aaacaactcc
     6601 agtaatgctc cacttagctg atggtggcga tacaggagca tttacttggt ggtttgatga
     6661 aatcacgagt cgaggtgtgt catttgattt aattggagtg tcgtattatc cgtattggca
     6721 cggttcaatg aatgaacttc aaaccaatat gaatgcgatc agccaacggt atggtaaaga
     6781 agtgatcgta gttgaaacag cttatgggca tacaacagcg aatgccgata ctatgccaaa
     6841 tgcttttggt gaagccgaag cagctgcggg gggatatgaa ccaacaccga caggacaagc
     6901 agagtatttg ctggatcttg ccgatcggat tcaggcagta ccgaataatc gcggggcggg
     6961 cttcttctat tgggaacctc tttggtacaa cggaaatgtg agttgggcaa cacaagcagg
     7021 tatgaattat ctcagtgttc aatccactat gggaaacgaa tgggacaacc aagcgatgtt
     7081 tgattttgct ggaaacgcct tgccaagctt gcgtgcgttt aaacaagcag gggcgcaaat
     7141 aaatctagtt aaaaatgcaa gtttcgaaag cgatggacca acgaccagtc cttccaattg
     7201 gcaaacctgg tttcaacaag gaaccgctaa gggtacgatc aaaacggaag cgggcacttt
     7261 ttttggggac tacaaactaa ctttttggca ggatcaagca tacgaggcat cagcttatca
     7321 aaatgttagt ggcataagca aaggaactta cacattgagt gcctatgtaa tgtctggggg
     7381 agagtttgat actaatcagt tatatatcaa aaattatggt ggagcggaac taaatactgc
     7441 ggttgttaaa acaagtgaat ggacaaaaat tacaatcaaa gacattccaa ttacaacagg
     7501 tgtttgtgaa attggggttt atagcaaggc ttcaggaaat gcttggacga acgttgattt
     7561 tgtttcattg gtgaaacaat agactgcgga tcgtttcgat tcaagatcgg aggttccctt
     7621 cagccacatc gttagcgtag aaggcaggaa tcgaacctgc acctcgcctt tgccaaaaat
     7681 aaccagaaac gatttccagt catttttacc aaaacgagta aagcaccaga cttcatctac
     7741 actttgtatt atgctaaaga gagttcaacg atttcgtaag aagcaatgaa aaaattgcaa
     7801 aaagttgtgc agataacata aagtttgttt aagaaagggt aaccttagaa ttccgtcgtg
     7861 aatatgattt gagcacttag atggatagaa aaaagttctc ggaaaacagt tcgtttttcg
     7921 agaacttctt tttatgataa gaatgaaaca aattaactta tattatagtc tcttttggta
     7981 gggaaccatc ttatttttaa tgaaaatcaa gcattttagc tattgaagaa tgttgattta
     8041 tagggattcc ccctccatat tatgttggct ctttattccc actccactgt tgctggtggt
     8101 ttagaagtga tgtcatacac gatacggttg acgtgggcta cttcgtttac gatacgtaca
     8161 gagatttttt gcaagacttc ccatgggata cgggcaaagt cagcggtcat tccgtcgatt
     8221 gaagttacag cacggatacc gactgtgtag tcgtaagtac gtccgtcacc cataacaccg
     8281 acagagcgga tccctggaag aacagtgaaa tattgccaga tatcacggtc aagtccagca
     8341 gctgcgattt cttcacgcaa gatagcatca gattcgcgta cgatttctag cttgtcttca
     8401 gtgatttcgc caagaacacg aataccaaga ccaggaccag ggaatggctg acgccaaacg
     8461 attgaatcag gcatgcctaa ctgagtccct aattcacgga cttcgtcttt gaataatgtg
     8521 tttaatggtt cgatcaattc aaattgcata tcttctggca gtccacctac attatggtga
     8581 gatttgatcg tctgtgctgt ttctgtacca gattcgataa cgtcagtata caacgttcct
     8641 tgtgctagga aagagacgcc ttcttcacca gcaagttttg ttgcttcgtc atcaaaaaca
     8701 tatacgaatt cattcccaat gattttacgt ttttgttctg ggtcagatac acctttcaac
     8761 ttgtctaaga agcgatctct tgcattgact ttgatgatgt tcaagccgaa ttttccgcct
     8821 agactttcca ttacttgttc cgcttcgcct ttgcgtagca gaccgtggtc aacaaagata
     8881 gatgtcaatt ggtcaccgat cgctttttgt aaaagaaccc caacgacact agaatcaacg
     8941 ccacctgaaa gaccaagcaa gacttttttg tcaccaactt gttcacggat tttttctact
     9001 tgcatatcga taaagttatc cattgtccag ttaccttcac agtgacaaac atcaaaggca
     9061 aagtggcgca gcaattcgat tccgtattcg gaatgacgta cttctgcatg gaattggata
     9121 ccataaaaat tacgtgttgt gtcttggata gaagcaatcg gacaatcttt gcttgttccg
     9181 actgtttcaa agccttcagg aactttagtt accaagtcac catggctcat ccatactgtt
     9241 tgttgttttg gtgtttcttt aaacaagaca gcttgatcgc ttgttacttc taacaaggct
     9301 ttcccatatt cgcggttttt agcaggctct acggaaccgc caagtttgta cgtgatcaat
     9361 tgcataccat agcaaatccc taatacagga atacctaaat tgaagatctc aggatcgatc
     9421 ccaaatgcat tctcatcata tacactgttt ggtccgccag aaagaatgat tccctttgga
     9481 ttcatcgctt ttacttcttc tgcagtaatg cggtggctca ataattcgga aaagactcca
     9541 agatcacgaa tacgtcttgt gatcaattgg ttatactgac taccgtagtc taatacgata
     9601 atcttttcaa cgttcgtcat atcggcaacg tttgtcaaat ttattacccc tatcccttta
     9661 aattttatct caaaatagtc aaactatttt aaaatgctaa tattttcgca tgaaaatcga
     9721 atcgatgata tgattttcag ttttatggag aattatatcg gctcttcctc gtgtaggcaa
     9781 gatatattcc tgtaaatttt tcaaattcac atttttccat acatcttttg ccattttaaa
     9841 ggcttcttca cgatctccga tagcaaaacg ataataataa ttctctggat tttggaaagc
     9901 cgtatccagt aacgcaccaa aacgatccaa ataccatttt tcgatcagca cgggatcggc
     9961 atcgacatag acagaaaaat caaaaaagtc gctgacgtag atttgctgat tcgcaggtaa
    10021 ttgaagcgta ttgatcccct caacgatcaa gatatcaggc tgctggatca attcgtactt
    10081 gtctggaatg atatcataca catcatgtga gtaaacaggt acttttatct cttcctgacc
    10141 agattttaca cggttcaaga aatcgatcag catctccata tcataacttt caggaaatcc
    10201 tttacggtcc atgattccac gctcttttaa tatacgattt ggataaagaa atccgtcagt
    10261 cgtgatcagc tgaacgtttc ttcgtttgaa ggtacgagac aaaatcgtct gcagcagtct
    10321 ggctgttgta ctttttccaa cggctacact tccggcgatc ccgataataa aaggcggtac
    10381 ttctacgtat ttatgaagaa ataaaccttt actcaagaac aaagattcaa attctttcat
    10441 ataaagataa atcagatgag caagaggtac atagatatct cgaacatcct gcatggaaat
    10501 gcggtcattc aaacttttga tattatctag ttcatcttgt gtcaaaggtg cttgcccatt
    10561 acgataaaat tctcgccatt cttctcttgc cacacggtag tagttcattg gatcattcat
    10621 attagcctcc tgcgttctcc catcaatgga actattttaa cacaaaataa acgcaaaccc
    10681 caccacttaa gagagattgc cgatcttttt gtataatctt tataatagaa agaaacggag
    10741 tcttattctt gacttgcgca cggatacata aaaggataga ctgttgtcat gaaagaaatg
    10801 agaaggatgg aagacatgac aaatcattac tattctgaac gcccagatac ggcgcacgat
    10861 tttgaacaat gggcttttga gttaaaaggc agaacctttc aatttgtaac agattctggt
    10921 gtattttcac gtgaaacagt ggattatggt tcacgtgtcc tgattgatgc tttcgattgg
    10981 gaagagttac ctgatgaagg aaagatctta gatgtgggat gcgggtatgg accgattggg
    11041 ttagctttgg catttgcgac acagcggttt gttgaaatgg tcgacattaa tgaacgagcg
    11101 gtaagtctag cgcaaggaaa cgcaaagcgt aaccaaatcg aacatgttga tatccatcaa
    11161 tcgaatatct atgaagcagt tcatgaagaa acctatgcag ctatcgtaag caatccgccg
    11221 atccgtgctg gaaaaaaagt tgtacatgaa attttgacag gcgcatatcc tcggttgaag
    11281 aagggcggta cactgacaat tgtgatccag aagaaacaag gagcgccgag tgcgcaaaag
    11341 aaaatggaag aaacatttgg taatgcagaa atcgtcacaa aagataaagg atattatatc
    11401 atcaaaagtg tgaaagaata actagctgaa aaatagtgga gcgacgaaag agtggaactg
    11461 ggcgtgcatg tacgggtacg tatcagttct ttttttgttg agaagttttt aaaaacaaag
    11521 aactattttt tcttcaaaaa gaaatagtag gaacagagtc gttgtttttt acgataaaat
    11581 aaataagata cagaacttgt atagaaggct gccaagtcgg tagataaagg aggtttttat
    11641 caaaaaacat actttccttt tttctctatt cttttcttgg gggttttggt acactgatag
    11701 ggaatttaga tagaagggag ccaataggat gaagctgacc atcagagata tcgcagaaat
    11761 ggcaggcgta tcagtaacaa ctgtctcaca gattttgaat aacaaaggaa gcagatttag
    11821 tgataagaca agaaaaaaag tgttgtcgat cgtgaatgaa catcattata agcccgatta
    11881 tttcgcttca aatgtgatca atcgacattc aaagaccatc ggaatgatcg tccctgatgt
    11941 cactgatttc ttcttctcca aactgatcga aggtgtagag agctatttga atcctttggg
    12001 ctatgtgatc atgttatgca attctcgtca tagccaagaa aatgagatca agtatttaca
    12061 agagctttcc caccgctcag tggacggaat cttgctggcc acaccaaata ttttacctga
    12121 gaaatatgca ttagacagcg gtttttatca aaaaatgcca atcattttaa tcgatcgcgg
    12181 attgaatcgt cgggataacg gacggcttat cgtaaaggaa tatgagggcg cctatcaagc
    12241 tgtttcgctg tttattgaaa atggccatac gaagatcggc atgctgaagg aaaccactgg
    12301 atactatcaa ttagaagaac gtttcaacgg atatcgtcat gcgctgaaag atcatggcct
    12361 gccttttaat gggaaatttg ttgaacatgg agagttgact gtccaaggtg gatatactgc
    12421 atcaaaaaaa ttactcaaac ataaagatat cacggctatt ttttgcggga atgatgccat
    12481 ggcgatcgga tgctatcagg cgatcgatga attgggcaaa aaaatcccag aagatatctc
    12541 agtcatagga ttcgatggat taaagctgtc agaatacatg attccgcaat tgacgacagt
    12601 gcagcagcca agttttgata ttggatttta tgctgcacgt ttcctgatcg atacgatcga
    12661 atttcctcaa agaaaagttc cgaacaaagt gttcgaaaca aaattaatta ttcgtgagag
    12721 cgtaaaagta ttgacaaagg gctaactcgg cggtatattg cttatgtaag catttacaaa
    12781 ggctagttag tgcttttttt atttttagta aaccagttta ctaaagtggt ttacctttcc
    12841 taaaaataat gtataatacc gaatgaagac aaggaggatc aggcaatgcg aatggtggat
    12901 ctgattgaaa aaaagaggga tggaaacgag ctgtcgaaag aggaaatcga gtacatcgtc
    12961 acgaactata cgaatggaaa gattccagat tatcaagtca gtgcattatt gatggcaatt
    13021 ttttatcaag acatgacaaa tgaagaaatc acgaacttaa cattggcgat tgctaattct
    13081 ggtgatgtca tcgatttgag ttctcttgaa ggaataaagg tagataagca ttcgacaggt
    13141 ggcgttggtg acacgaccac attgatttta gcccccctag tagctagtgt cggagtgaca
    13201 gtggctaaaa tgtctggacg cggtctaggt tacacaggtg gtacactaga caagctagaa
    13261 gcaattcccg gatttcagat cgagctttcg gatgaagaat ttgtacgcat tgttaatgaa
    13321 agtaaagtag cggtaattgg tcaatcagga aacttggcac cggcggacaa aaaattgtat
    13381 gccttgcgtg acgtcacagc gaccgttgat tctcttccgc tgattgccag ttcgattatg
    13441 agtaaaaaaa tcgcggcagg agcagatgca atcgttttgg atgtgaccac tggagacggt
    13501 gctttcatga agaacatcga agatgcaaga cgtctggcaa agaccatgac gagcattggg
    13561 aaactggcta accgagaaac agtggcggtt atctcagata tgtcagaacc tcttggcgaa
    13621 gctatcggaa atagtctaga agttgtcgag gcaatcgaaa cattacaagg aaatggtcca
    13681 gaagatctag tagaaatgtg ctacgcctta ggaagccaaa tggttgttct tgctggcaaa
    13741 gctaagacaa ccgatgaagc aagaacgttg cttcaagaag ctctggaatc aggtaaagcg
    13801 ttagcaaaat tcaaagaaat gatccaaaat caagggggag acccagcgat cgtggaacat
    13861 ccagaacgaa ttttgactgc acgctacaca atggagcttc ctgctaaaca aagcggagtt
    13921 gtgtcaaaaa tcgtggcaaa tgaattagga attgctgcaa tgatgctagg tgctgggcgt
    13981 aaaaccaaag aagaagatat tgatcacgct gttggattga aactgcataa aaaaattggt
    14041 gacactgtga caaaaggtga gtcactattg acgatctata gtaacgacga agaaatcact
    14101 tctgtcattg aattattata taaaaatatt gaaattggtg aatcggcaat gaagccaacg
    14161 ttgattcatg acatcatcac agaataaagg aaaaaagcgg actttaccgt tttaaagaaa
    14221 aggggaaaag aaatggaact aaaccgaatg atcgaccata ctattttgaa agcagacgca
    14281 tctaaagaag ctgtgatgca aattatcgaa gaagcaaaaa aataccattt ttattctgtc
    14341 tgcatcaatc cagcatgggt tgcgctagcg aaagaacaat tacaaggtga gccagtagcc
    14401 gtatgtacag taatcgggtt tcctctagga gcaaacacac ccgagacaaa agcatttgaa
    14461 acaacagatg ccatcaataa cggtgcagat gaagtcgata tggtcatcaa tatcggtgct
    14521 ttgaaatcaa aggatgatca acaagtccaa aaagatatcg aagcagttgt tgaagcagca
    14581 aaagatcgtg cgcttgtcaa agttattatt gaaacatcgt tattagatag agaagaaatc
    14641 atccgagctt gcgaaatagc aaaagctgca ggcgctgatt ttgtcaaaac atcaactggt
    14701 ttctcaacag gcggagcaaa ggtagaggat gtccgtttga tgcgtgaaac tgtaggacca
    14761 gaaatgggtg tcaaagcttc cggcggtatc cataacgaag aagaagcaat ggcaatgatc
    14821 gaagctggtg ctacaagaat cggaacaagc gcaggagtag caatcgtatc tggttcaact
    14881 ggtgaaggct attaattttt tcaaaggaga agctcatggc agaactcaaa caagaatgga
    14941 tcaacacagc tattgaagcg ttagataaag cctatgttcc ttattcccat tttccggtag
    15001 gcgcttgtct cgtaacggaa tcaggcaaga tttatcaagg catcaacatt gagaatgcat
    15061 catttggatt aacgaattgt gccgagcgca cagctttttt caaagcagta tctgaaggag
    15121 aaaaaacatt tacgcatcta gtcgtagcgg ggcacacccc cgatcctatc tctccttgtg
    15181 gtgcatgtcg acaagtaatg gcagaattct gttcccctga tatgccggtt acgctagtag
    15241 gagacaatgg tgtgacgaag gcaacgaccg ttcgtgcact gctgccttat gcatttactg
    15301 aaaaagatct ataactcatg ttcattcagt cagctgactg ttgaaaaata aaacaattat
    15361 tttaggaggc tttacagaaa tgaaaaaagc aaaattattt ggtttaggtg ctgtcgcact
    15421 agcagcaggg ttatttttag gtgcatgcgg caacaacggc tctactgatt catccggtgg
    15481 caaaagctca tcagacacaa caactgcagc attgatcaca gacacaggtg gtgtagacga
    15541 ccgttcattc aaccagtcag cttgggaagg actagaaaaa tgggggaaag atcaaggact
    15601 ttcacgagga aatgatggct tccaatattt ccaatcttca aatgaatcag attacatccc
    15661 aaatattgac caagcattga atgctggatt caaaacgatt tttggtattg gttataaact
    15721 aaaaccagcg atccaagaac aagcaacaaa caatacaggt acgaactttg ttattatcga
    15781 tgatgtcatc gatggattag ataacgttgt atctgcaact ttcaaagaca acgaagcttc
    15841 ttatcttgca ggtgttgcag cagcttatac gactgaaaca aacgtagtcg gctttatcgg
    15901 cggtgtaaaa ggtgaagtaa tcgatcgttt tgatgctgga ttcaaagccg gtgttgatgc
    15961 aggtgcgaaa gaattaggaa aagaaatcaa agtattgaat caatatgctg gtgacttctc
    16021 tgctccagat aaaggacgtt caattgctca aggtatgtat gcacaaaacg cagatatcat
    16081 cttccatgct tcaggcggta caggtaacgg tgtattccaa gaagcaaaat cattgaacga
    16141 atcaggcgac aaaaaagttt gggttatcgg tgttgaccgc gaccaatcag acgaaggcga
    16201 atacacatta aacggcgaaa agaaaaactt cacattgaca tctacactga aagcagttgg
    16261 aacagtagta gaagatttgg cacaaaaatc agcggacggt aaattccctg gcggcgaaca
    16321 cactgtttat ggattgaaag aagacggtgt tggtctaaca gaaggacaac tttctgacga
    16381 agcgaaaaaa gcagttgatg aagcaaaaga aaaaatcatt tcaggtgacg taaaagttcc
    16441 agaaactcct gaagaaaact aaaaccattt ctatagcctt gttagacaca tgtttgacaa
    16501 ggctatggtt tgattagaac aagaattgtt catttatcca agctgccttg tgacagtttg
    16561 gataagggaa caactccctg aagtaatgtt tgtttcaaaa gaagaacgct cactaagcgt
    16621 gaatcttagt caatcgttgc cctagataga aatcgttagg atgtgaaaaa aaggtggttc
    16681 aagaaaatat tgtcatagaa atgcagaata ttaccaaaga atttggaaca ttcaaggcaa
    16741 atgacaacat caatttacaa gtaaaggcag gagaaattca tgcgttgctt ggagaaaacg
    16801 gtgctggcaa atctacattg atgaacgtgc tttccggatt attagagccg acatcaggga
    16861 aaattttgat gcgtgggaaa gaagtaaaga tcacaagtcc gaccaaagcc aaccaattag
    16921 ggattgggat ggtccatcag cactttatgc ttgttgatgc ctttactgta acagaaaaca
    16981 tcgtgttggg aagcgagcct agtcgcgcag ggatgcttga ccataaaaaa gcacgaaaag
    17041 agatccaaaa agtttctgaa cagtatggat tgtcagtcaa cccagatgct tacgttcgtg
    17101 atatttcagt tggaatggaa caacgggtag aaattttaaa aacactttac cgaggagcag
    17161 atgtactgat ttttgatgag ccgacagctg tattgacccc tcaagaaatt gatgaattaa
    17221 tcgtgatcat gaaggaatta gtcaaagaag gcaagtcgat cattttgatt acgcataagt
    17281 tagatgaaat caaagcagta gctgaccgct gtacagttat ccgccgtgga aaaggaatcg
    17341 gtacagtcaa cgttaaagac gtaacctcac aacaattagc cgatatgatg gttggaagaa
    17401 cggtttcatt caaaacgatg aaaaaagaag cgaagcctca agaagtcgtt ttgtctattg
    17461 aaaatctagt ggtgaaagaa aatcgtggat tagaagccgt gaaaaacttg aacttagagg
    17521 ttcgtgctgg cgaagtactt ggtatcgctg gaatcgatgg aaacgggcag tcggagttga
    17581 tccaagcttt gactggtttg cgaaaggcag aaagcggaca tatcaagcta aaaggggaag
    17641 acatcactaa taaaaaaccc cgaaaaatca ctgaacatgg tgtaggacac gtgccagaag
    17701 accgtcataa atacgggtta gtcctagata tgacattatc tgaaaatatc gcactacaaa
    17761 cctatcatca aaaaccttac agtaaaaacg gcatgctgaa ttattcagta atgaatgaac
    17821 atgccagaga attgatcgaa gaatatgatg ttcgaacaac gaatgaactt gttcccgcaa
    17881 aagctttatc aggcggaaat cagcaaaaag caattatcgc tcgtgaagtc gaccgagatc
    17941 ctgatctgtt gattgttgca aatccaactc gtggactgga tgtaggtgcg atcgaattta
    18001 ttcataaacg tctgatcgaa caaagggaca aatacaaagc agtgttactg atcagtttcg
    18061 aattagaaga aattttaaat gtttcggatc gtattgctgt tatccatgaa ggagaaattg
    18121 tcggtatcgt tgatccgaaa gaaacatctg aaaatgaatt aggtctattg atggcgggct
    18181 ataccttaga agaagcacga aaagagttag aaaagggggc tggcgcacat gaatgatcgc
    18241 agcgaaaaat tccgtaattt attagtgcct gtactttcag ttgttttagg attgatctta
    18301 ggcgcgatat tgatggctgc ttttggtttt cagcccatcc aaggttattc ttctatgcta
    18361 gatgcagccc ttggttcgca aagagggata ggtgaaacgt taaggcaagc aacgccgttg
    18421 atttttacag cattaggttt ttctgtagct aactcagctg ggtttttcaa tattgggctt
    18481 tctggacaag cactttgcgg gtggatcgtc agtgtctggg tggcacttgc gtttccagat
    18541 ctgccaaaag ttgttttgct tccgttatgc gtgatcttag gtgccttagc tggtgcgatg
    18601 gcagcggctg ttccaggttt acttcgagca ttttttggaa caagtgaagt gatcgtaaca
    18661 atcatgatga actatatctt attatacgta agcacatttg ctttacatga agtgatgcct
    18721 gaaagctatc gctcaagttt ggactcttcc aatatgatca gtcaaaatgc ctcactgcgg
    18781 ttaccatggt taacacaagc ttttggcggc tctcgcgtaa actcaggatt atttttagca
    18841 atcatcgcgc ttattttcgt ttggctgctg atgaagaaaa cgactttagg atttgaaatc
    18901 cgttctgttg gattgaatcc ttttgcttct gagtatgcag gaatgagtag taaacgtacg
    18961 atcgttttgt cgatggtcat ttctggcgct ttggcagggc ttggcggtgt ggttgaagga
    19021 ttaggaacgt atcaaaactt ctttgttcaa acgacttcgc tatctattgg atttgacgga
    19081 atggctgttt ccttgcttgg ttctggatca gcaatcggta ttcttttatc agcgatcttg
    19141 ttcagtattt tgaagatcgg tggacttggc atgcagacag gagcaggtgt tccgtttgag
    19201 atcgtcaatg tatcgattgc tttgattatc ttctttgtcg ggatcaactt cctgattcgt
    19261 ttcgtcatgg ctaaagtctt ccaagggaaa aaacaagaag agatcgttgc gacaatagaa
    19321 tcgaaaccaa cgaatcaaaa ccatgaaggg ggcgaactat aatgtcgact atcgaattaa
    19381 ttgccttgat ccttacttct acactggttt attcaacacc gctaattcta acgtcactag
    19441 gcggaacttt ttctgaacga agcgggatcg taaacgtagg gctagaggga atcatggtca
    19501 tgggtgcttt tagttccatc gttttcaact tgacgatgag tccatcactt ggttctatga
    19561 caccatggat cgctatttta gtgggtggcg tcgtaggtat cctattttct ctgattcacg
    19621 cagtagcaac agtcagtttg cgtgctgacc atattatcag cgggacagta attaatctga
    19681 tggcacctgc tttaggtgtt ttcttgatca aagcatggta tggtaaaggt caaactgata
    19741 acatcacgca gaatctgggg tatttttctt tcccaggatt agcagatatt ccggtcattg
    19801 gaccaatttt ctttaaaaat acattcttac ctgcttattg tgctattttg attgctgtcc
    19861 tagcttgggt gatcatcttc aagacgaaat tcggcttgcg cctccgttca gttggtgaaa
    19921 atcctcaagc tgccgacaca ttaggtatca atgtatatgc tatgcgttat tcaggcgtac
    19981 tgatttctgg cttgttaggc ggtatgggcg gtgctgtttt tgctcagtcg atttccggta
    20041 acttttcagc tggtacgatc gttggacaag ggttcatctc catggctgct atgatctttg
    20101 gtaagtggaa tcctttaggg gcaatgggtg cctcattgtt ctttggtttt gctcaaagct
    20161 tgagtatcat tggcgcacaa ttaccagtta tttcttcgat tccgccagta ttgctgcaaa
    20221 ttgctccata tttgctgaca atcattgtgc tggttgtttt cttagggaaa gcttctggtc
    20281 caaaagcaaa cgggaagaac tatatcaaat caaaataatc ttttagcgaa taaaaagggt
    20341 gggttacggg aagtgttttt cgtctcgccc tttttgtttt aaaggcaata aaaataaaaa
    20401 gaaagaagga ataatcatgt ttaaacgcgt acatctgatc gtcatggatt cagtaggaat
    20461 tggagaagca ccagatgccg ataagttcgg tgatgttggt tctgatacat taggacacat
    20521 tgcgaaagaa gcaggattga acgttccaca tttagagcag cttggtttag gaacgatccg
    20581 tcctcttgaa ggtgtggaat cagtgaaaga tcatcaaggc tatgcgacga aattagaaga
    20641 agtttctgta gggaaagata cgatgacagg ccactgggaa atcatgggat tgaatatcaa
    20701 aactccattt cgtgtgtttc ctaatggatt ccctgatgat ctgatcaaac aaatcgaaga
    20761 attttctggc cgtaaagtcg tatgtaacaa accttatagc ggaacagctg ttattgacga
    20821 ttacggtgaa caccaaatga aaacaggcga tttaattgta tatacttctg ccgatcctgt
    20881 ccttcagatc gcggcacatg aagaaatcat tcctttagaa gaattatatc ggatttgcca
    20941 attcgttcgt gatataacaa aagatgatcc gtatatgatt ggccgaatca ttgcacgtcc
    21001 gtacgtgggt gaaccaggag acttcacacg aacaagcaat cgccatgatt atgcgttgaa
    21061 tccattcgga cacacagtcc ttgattcttt gaaagaagca ggaaaggacg tgattgctgt
    21121 tggaaagatc aacgatattt tcaacggaca aggaatcact gaatctgtcc gcacgaaaag
    21181 caatatggat ggcgttgacc agctgctgaa agttatgaaa aacgattttt caggaatcag
    21241 ctttaccaat ttagtagatt ttgatgcatt atacggtcat cgtcgggatg tagtcggcta
    21301 tgcgcatgcg attgaagaat ttgacttacg aattccagaa ttgcttgaag cgatggaaga
    21361 agacgacttg ctaatgatta cagccgatca cggaaacgat ccgacgttta ctggtaccga
    21421 tcatacgcga gaatatgtac cgttattggt ttacagtaaa aaaatgaaag aacaaggggc
    21481 attgcctcaa ggatattatt cagatatttc agcaacgatt gctgagaact ttagtgtttc
    21541 ttcgactgaa aatggcgaaa gcttcttgaa attattaaaa taatcttttt aaataggaga
    21601 aagcaaatga ccaagttaag cgagatgtta caagaaacga cccagtttat ccttgataaa
    21661 ggagtaaaag aaattgattt tggtttgatt ctaggttcag gattaggtga attagcagat
    21721 gaaatcgaag atgctgtaat gatttcttat gatgagatcc cgttctttcc tgtttcgaca
    21781 gtagttgggc atgcaggaaa gctagtttat gggtctctgg caggcaagaa agtgttagcc
    21841 atgcaaggga gattccacta ctatgaaggt cattccatgc aaacagtgac ctatccagta
    21901 cgtgtgatgg ccggactagg ggcccattca gtcattgtga ctaatgcatg tggtggtgtc
    21961 aacgaatcat ttgtaccagg agatttgatg ctgattaccg atcatatcaa ttttactggt
    22021 gaaaatcctt tgatcggtcc gaatgaagat gcaatgggcc ctagatttcc agatatgagt
    22081 caagcttata cgcctgacta tcaagcaaca gcaaaagcag cagcagaaaa actaggtttg
    22141 gatcttaaag aaggcgtata tatgggctat tctggaccaa cttatgaaac gcctgcggag
    22201 atccgaatgt ctcggacaat aggagccgat gcagtaggga tgtcgactgt tccagaagtc
    22261 atcgtagcag ctcacagcgg attaaaagtg ttaggaatct cttgtattac caacttagca
    22321 gcaggtatgc aagcgaatct gagccatgaa gaagttgtag aaacaactca gagagtcaaa
    22381 caatctttta aagcattggt taaagaaaca ttgacattat tgtaatagga ggaacaataa
    22441 aatgagtgtg catattgaag cgaaagaagg cgagatcgcc gataagattt tacttccggg
    22501 agatcctttg cgagctaaat atattgcgga gacttttttg gaagatccag tttgttacaa
    22561 tcaggtacgc ggtatgttag gctttaccgg caaatataaa ggagaacgta tttctgttca
    22621 aggaacagga atgggaatgc catcagccac catctatgcc catgaattga tccagtcgta
    22681 tggtgtaaag aaattgattc gagtaggtac atgcggtgca ttgtcaaaag acgtccatgt
    22741 tcgtgattta gtactagcac aaggtgcagc gaccagttct tcgatgatcg aaaaaaattt
    22801 ccaagccttt cattttcctc caatcagtga tttcaatcta ttactgaaag catatgagat
    22861 agctaaagaa aagggctata cggttcatgt aggaaatgtt ctttcggaag attctttcta
    22921 caaagatgat ttgacagaga cgttccaatt agctgaactt ggcgttttag gagtggaaat
    22981 ggaagcagcg gctctttatt acttaggcgc gaaataccat gtgcagacat taagtttgat
    23041 gaccgttagc gaccatttga tcaccggtga agaaacaaca gctgcagaac gtcaatctac
    23101 gttcaatgaa atgatcgaag taggattgga aacagcgata gcagaataag aaggaaatga
    23161 aaaagctgcc ctgcattaag taataagaac atccaagcaa aaatagctcc tcatgttttt
    23221 gacttggctg gacttattat tgcaaatgta agcttttgaa caccgtttac caagaggttg
    23281 tgaatctgcc gtttaactct gagtcacaag gaacaatttc aaaaaacagc ttttcatgtt
    23341 tgatgaatat tgtgacttgt gaccgagaag ttggcaggag aaaaccgttt atacgaaaaa
    23401 agggactgtg acaaatcgtg tcacagtccc ttttttatag tgttttttat aggtagtatt
    23461 ttttagcaac tgttaagtca tcatttaatt cataaacaag tggttgacct gttggaattt
    23521 caagatccat gatgtcttcg tcagagatac cttcgatatg tttagctagg gcgcgtaatg
    23581 agttaccatg tgcagctact aaaacagttt tgttgtctag taatgctgga gcaatttcat
    23641 cttgccagaa tggcaaagcg cgttctaatg tcactttcaa gttttctcca ccaggaatat
    23701 cgcgtttgtc taacattgcg taacgacggt catttgctgc agaaccttcg tcagtttctt
    23761 ccattaatgg aggaagagta tcgtaagaac gacgccagat gtgcacttgt tcgtcgccgt
    23821 atttttcagc tgtttctttt ttgttcaaac cttgtaattt accatagtga cgttcgttta
    23881 agcgccatga tttgatttgt ggtacccaaa gttgatctga atattctaag atcaagttac
    23941 atgttttgat tgcacgagtt aaaacagaag tgtaagcaac atcaaattcg attccagctt
    24001 ctttgatttt gcgtccgcct tcttttgctt cttcgattcc ttctggtgct agatttacat
    24061 cagcccaacc agtaaattgg ttcaatgcat tccattcact aagaccgtga cgagaaaaaa
    24121 ctaattttgg catgtttagc tctcctttta agataggttt atatttctga aattttcagt
    24181 tctgttttat tgtacaattt tttggaagag atttccatcg aaaaattaga taagattcta
    24241 tgagaaaaca agtagagaat ataaaaaaac gaacagtgcc cacgcaactg ttcgttttta
    24301 ggtattagat gcataattgc tcgatttctt tttcaggtaa attgatatat ttataacatg
    24361 ttccaactga aacaccgcat tttgaagaaa taaattggat cgtttctttt ttttcataat
    24421 agagacgtcg gatttttttg atcgtttttg catcgatttt tggtcttccg ccgatttttc
    24481 cttttttacg agcttcatct aatccaacta atgtacgttc tttgatcaat gcgcactcca
    24541 tgtttgctag gttttccatt aattggaaat agatttttcc catttcgcgc gtatcgattc
    24601 cttcatcaag actaatcaag tgcagcccgc gatctttgaa taattgcgtg agttctgtta
    24661 attgccgcgt agatttgcct aatcgatgaa gttggcaaat gactagtgca tcgccttgtt
    24721 gcatgtcagc gatcactgac tccaaatgaa cagcagtaga atcattttgt tccacttcat
    24781 atgtctcatg gaaaatttct tgacaaccaa agtcagataa ttgatcaacc tgagttgtta
    24841 aatcaacgtc atttatggtt gtgcgtgcat aaccaataat tttcatggtg attcctttct
    24901 agcagtgatt ttctaactgg atataacagt aaaaatgtag cataaagtac gtcaaaaaga
    24961 tagttttttc gcaaattttc cgctgtcttt ttttattgga tgatcaagca gtttttcttt
    25021 atttacgacg gattgcgtca agaatctcaa cgcctttttc tgagccgaca actactttag
    25081 aaaccatgtt taggaaaagc ccatgttcta ctaccccaac aagttcatct aaataggtag
    25141 ctaagctttc tggctgttcg atgacttcta gatgcagatc aatgatgtaa tgaccaccat
    25201 ctgtggttaa gacttctccg ttttcgtctg tacgcaagac gggttggaat cttttttctt
    25261 caaaaatatg aagtagctgt tgggagccat aagggatcac ttcaactggg agtgggaact
    25321 tcccaagttt atcgactaat ttagaagaat caacgatcca aatcgtttct tttgagtacg
    25381 tagcgactat tttctcgaaa agcaaagcag cgccgccgcc tttgatccct tgaaaatcag
    25441 cactgatttc atcagcaccg tctattgtca aatcgactac ggggacttca tcaatactct
    25501 ttaacggtat tcctaatttt tccgcttgtt cttttgtcgc atttgaagta gtaactcctg
    25561 tgatacgtaa accttcttca cgcatacggc gaccaatctc ttctaccata taataggcgg
    25621 ttgaaccggt cccgagacct acgatcatcc cgtcctttac gaatttcgcg gcttcaatgc
    25681 cagctaattg ttttaaattc atctttgcgt ccaccttttt ttagtttgag catcgtgctt
    25741 tcctcactat tttagcgtga tttacggtaa aaagatacca ttctttttta ttccaataaa
    25801 gctttttcaa aaaaaattgc atctcttgtt gacatgtttt ttcattcgtg atattctaac
    25861 aaaggtgctt tatctacagg tttctgtgaa gaaacgtgct gactgtttat caaagcatat
    25921 ttgataagga tgcagacatt gcatttattt tttatcacaa aaaaagaacc acctggatgt
    25981 gtggaactag acgcaaatcg tgggaaggag gaaacaagga atgcctacaa ttaaccaatt
    26041 agtacgtaaa cctcgtaaat caaaggtgga aaaatctaat tcacctgctt tgaacaaagg
    26101 atataacagc tttaagaaaa ctcaaactaa cgtaaactca cctcaaaaac gtggggtagc
    26161 aactcgtgtg ggtacaatga cacctaaaaa accgaactca gctttacgta aatatgcacg
    26221 tgttcgtttg tcaaacttaa ttgaagttac tgcttatatc ccagggattg gtcataactt
    26281 acaagaacac agtgttgtat tattacgcgg tggtcgtgta aaagacttac caggggtacg
    26341 ttaccatatc gttcgcggtg ctttagatac tgctggtgtt aacgaccgta aacaaagccg
    26401 ttctaaatac ggaactaaaa aacctaaagc ttaattttaa ttattaaaca aatgaaactt
    26461 gcttctacta tatagaagta attactatga acaatatttc ggaaggagga gttacggatg
    26521 ccacgtaaag gtcctgttgc aaaacgtgat gttttaccag atcctattta taactcaaaa
    26581 ttagtaactc gcttgatcaa ccgtgtaatg gttgacggaa aacgcgggat tgcagctaac
    26641 attatctata atgcctttga cttgatcaaa gaatctacag gtaacgatcc attagaagtg
    26701 tttgaacaag caatgaaaaa cgttcaacct gttcttgaag ttaaagcacg tcgtgttggg
    26761 ggttcaaact accaagtacc agttgaagtt cgccctgaac gccgcgttac tctaggtctt
    26821 cgttgggtag taaactacgc tcgcttgcgc ggtgaacaca caatggaaga acgcttagca
    26881 aaagaaatca tggatgctgc aaacaacaca ggtgcttctg ttaaaaaacg tgaagacact
    26941 cataaaatgg ctgatgccaa ccgtgcgttt gctcactatc gctggtaaga ttctctctgt
    27001 ttggtcggct tttataagtc gacctcagat agtgtcctta tttttagcaa ctagatagta
    27061 aagagaggag aaacaaggag aatgaccaga gaattttctt tacaaaacac tcgtaatatc
    27121 ggtatcatgg cccacatcga tgctggtaaa acgactgcta ctgaacgtat cttgtactat
    27181 actggtaaaa tccataaaat tggtgaaact catgaaggag cttcacaaat ggactggatg
    27241 gaacaagaac aagaacgtgg tattacgatc acttctgctg ctactacagc acaatggaag
    27301 ggtcaccgaa tcaacatcat cgacacacca ggacacgtag acttcacagt tgaagttgaa
    27361 cgttcattgc gtgtacttga tggtgctgta actgttcttg atgcacaatc aggtgttgaa
    27421 cctcaaactg aaacagtttg gcgtcaagca acaacttatg gtgtacctcg tatcgtattt
    27481 gcaaacaaaa tggacaagat cggtgctgac ttcttatatt cagtatcaac tttacatgac
    27541 cgtttgcaag caaacgctca cccaatccaa ttaccaattg gtgcggaaga cgacttcaca
    27601 ggaattatcg accttgtaac aatgaaggct gaaatgtata caaacgatct tggaacagag
    27661 atcgaagaaa cagaaattcc tgaagaatac cgcgaattag ctgaagaatg gcgcgaaaaa
    27721 ttggttgaag ctgtagctga aactgatgaa gaacttactt tgaaatactt agaaggtgaa
    27781 gaaatcactg aagctgagtt aaaagaaggt atccgccgcg caacagttaa cgtagaattc
    27841 tacccagtac tatgtggttc agcatttaag aacaaagggg ttcaattact attggacgct
    27901 gttcttgatt acttgccatc accgttagat atcccagcta tcaaagggat tgaccctaaa
    27961 actgatgaag aagtagaacg tcctgcagac gacagcgctc ctttctcagc tttagcattt
    28021 aaagttatga ctgacccatt cgtaggtcgt ttaactttct tccgtgtgta ctctggtgtt
    28081 ttacaatcag gttcatacgt acaaaacgca acaaaaggca aacgtgaacg tgttggtcgt
    28141 atcctacaga tgcatgccaa ctctcgttca gaaatctctg aagtttacgc tggagatatc
    28201 gctgctgctg ttggtttgaa agacacaact actggggaca cactatgtga tgaaaagagt
    28261 ctagttattc ttgaatccat ggaattccca gaaccagtta tccaagtcgc tattgaacct
    28321 aaatcaaaag ctgaccaaga taaaatgggt gtggctttac aaaaactatc tgaagaagat
    28381 cctactttcc gtgctgaaac aaacgtggaa acaggcgaaa caatcatcgc tggtatgggt
    28441 gaacttcact tagatatcat cgttgaccgt atgcgtcgtg aattcaaagt tgaagctaac
    28501 gtaggtgcgc ctcaagtatc ttaccgtgaa actttccgtg ctggcactca agccgaaggt
    28561 aagtttgtac gtcagtctgg tggtaaaggt caatacggtc acgtatggat tgaatttaca
    28621 ccaaatgaag aaggtgctgg tttcgaattt gaaaatgcga tcgtcggtgg ggttgttcct
    28681 cgtgaataca ttccagctgt tgaaactggt ttgaaagatg ctatggaaaa tggtgtctta
    28741 gctggctatc cattagtaga tatcaaagct aaactttacg atggttcata ccatgatgtc
    28801 gattcaaatg aaactgcctt ccgtgttgct gcttctatgg cattacgtgc agcagctaag
    28861 aaagctaatc cagtaatcct tgaaccaatc atggctgtag aagttgttat ccctgaagat
    28921 tatttaggtg atgttatggg tcacgtaact gctcgtcgtg gacgtgttga aggtatggaa
    28981 gctcgcgctg gcggccaaca agttgttcgt gcgatggttc cacttgctga aatgtttggt
    29041 tatgctacaa ctcttcgttc tgctactcaa ggtcgtggta catttacaat gacatttgac
    29101 cactacgaag atgtaccaaa atcagtacaa gaagaaatca ttaagaaaaa cggcggaaaa
    29161 gctgaataat ttcttgaaac cggatagtcc tgcaaagaga ttatccaaag agactatatt
    29221 tttaatatag tttcgtgtaa aataattatc gatgatatgg gcatgtggtg taccttgtgt
    29281 acatcatata cctatcagac aaataaaaaa tatattgtat ttttaggagg acattttaaa
    29341 atggcaaaag aaaaatttga ccgttctaaa ccacacgtta acattggtac tatcggacac
    29401 gttgaccatg gtaaaactac attaacagct gcaatcacaa ctgtactatc taagaaaaac
    29461 ggcggccaag ctatggctta cgatcaaatc gatggtgctc cagaagaacg cgaacgcgga
    29521 atcacaatct caacagctca cgttgagtat gaaacagata cacgccacta cgctcacgtt
    29581 gactgcccag gacacgcgga ttacgttaaa aacatgatca ctggtgctgc tcaaatggac
    29641 ggagctatct tggtagtttc tgctgctgac ggcccaatgc ctcaaactcg tgaacacatc
    29701 ctattgtctc gtcaagttgg tgttccttac atcgttgtat tcttgaacaa agtagacatg
    29761 gttgatgacg aagaattact agaattagtt gaaatggaag ttcgtgacct attaacagaa
    29821 tacgaattcc ctggtgacga tgttcctgta gttgctggat cagctttgaa agctctagaa
    29881 ggcgacgctt catacgaaga aaaaattctt gaattaatgg ctgcagttga cgaatacatc
    29941 ccaactccag aacgtgacaa cgacaaacca ttcatgatgc cagttgaaga cgtgttctca
    30001 attactggac gtggtactgt tgctacaggt cgtgttgaac gtggacaagt tcgcgttggt
    30061 gacgaagttg aagttgttgg tattgctgaa gaaacttcaa aaacaacagt tactggtgtt
    30121 gaaatgttcc gtaaattgtt agactacgct gaagctggag acaacattgg tgctttacta
    30181 cgtggtgttg cacgtgaaga catccaacgt ggacaagttt tagctaaacc aggtacaatc
    30241 acacctcata caaaattctc tgcagaagta tacgtgttga caaaagaaga aggtggacgt
    30301 catactccat tcttcactaa ctaccgtcca caattctact tccgtacaac tgacgtaaca
    30361 ggtgttgttg aattaccaga aggaactgaa atggttatgc ctggcgacaa cgtaacaatg
    30421 gaagttgaat taatccaccc aatcgctatc gaaaacggta ctaaattctc aatccgtgaa
    30481 ggcggacgta cagttggtgc cggtgtcgtt acagaaatca aagcttaatt cacttaagtt
    30541 ttaaagtaaa gcaacattta ttcggacgta atcgctcgga acgaaggtct taggatcttt
    30601 gttccgagct tttttattta ttgcaggatc aggaaaaaag acaggagtat tataaataga
    30661 agaaacacga aataaaagag tcagtatttt ctaaatgaaa ctttatgaaa acaaaatgtt
    30721 ttttacaaga aaaagccttg ctttgtgagt ttatatatat tataatgaca aaggtgcgtt
    30781 tactttagtg aattgcacag acgtaatcag aatgatttcg tcggctaaga aacgagaggt
    30841 tgcgacacgc ccggacgctt tgccacgaac gagcgtgacg gaaattttcg tggagctatg
    30901 tctacttttc aaaataggcg aaggagggaa caagatggca aaacaaaaaa ttcgtatccg
    30961 tttaaaagcg tatgaacacc gtattttaga tcaatcagcg gataaaatcg tggaaactgc
    31021 aaaaagaact ggagctagcg tgtctggtcc aattccatta ccaactgaac gcagtcttta
    31081 cacaatcatc cgtgcgactc ataaatacaa agattcacgc gaacaattcg aaatgcgtac
    31141 acacaaacgt cttatcgaca ttgtgaaccc aacaccaaaa actgttgatg ctttgatgaa
    31201 gctagactta ccatctggtg taaacatcga aatcaaatta taaaactaaa acaaaaataa
    31261 tggaggtgta ctcatgacca aaggaatctt agggaaaaaa gtgggaatga cacaaatctt
    31321 cactgaatct ggtgaattaa ttccagtaac tgttgtagaa gctactccaa acgtagtact
    31381 acaagtgaaa acaatggaaa cggatggcta cgaagctatt caagttggtt accaagatat
    31441 gcgtgaagtt ttatcaaaca aacctgcgaa aggtcatgtt gcaaaagcaa acacggctcc
    31501 taagcgcttc attcgtgaat tcaaaaatgt tgagctagga gaatatgaag taggaaaaga
    31561 aattacagta gatgttttcc aagcaggaga cattattgat gtaacaggta ctacgaaagg
    31621 taaaggattc caaggggtta tcaaacgcca cggacaaagc cgcggaccaa tggctcacgg
    31681 ttctcgttac catcgtcgtc ctgggtcaat gggtccagtt gcacctaacc gtgtatttaa
    31741 aaacaaacgt cttgctggcc gtatgggtgg caaccgcgta actatccaaa acttggaagt
    31801 tgtacgtgtc gatgcagaaa gaaatgttat cttgatcaaa ggtaatgttc ctggagctaa
    31861 aaaatcttta attactatca aatcagctgt gaaagctaaa taattgtagg aagaaaggag
    31921 gaactaagga atgccgaatg tagcattatt caaacaagat ggaagccaaa acggtgaaat
    31981 cacacttaac gaagaaatct tcggaattga acctaatgaa agtgttgttt acgatgcaat
    32041 cgtaatgcaa cgtgcgtcat taagacaagg aacacacgcg gttaaaaacc gtagcgctgt
    32101 tcgcggcggc ggacgtaaac catggcgtca aaaaggaaca ggtcgtgcac gtcaaggatc
    32161 tatccgctca ccacaatggc gtggaggtgg cgtagtcttt ggaccaacac cacgttctta
    32221 cagctacaaa cttcctaaaa aagttcgtcg tttagcaatg aaatctgtat tgtcagaaaa
    32281 agttgctgaa aacaacttgg tagcaatcga aggcttgaac ttcgacgcac caaaaacaaa
    32341 agaattcaaa caagttcttg ctaacttatc tatcgactct aaagtattgg ttgttctaga
    32401 aaaaggtaac gactttgctg ctttatcagc acgtaactta ccaaacgttt ctgtagtaac
    32461 ttctgataat gtgagtgtgc tagacgttgt atcaaataca aaagtgttag caacacaaac
    32521 tgctcttact caaattgagg aggtgcttgc ataatgaact tactagacgt aatcaaacgc
    32581 ccagtgatca ctgaaaaatc aatgcttgcc atggatgaca agaaatacac ttttgaagtg
    32641 gacactcgcg caaacaaaac tttagtaaaa caagcggtag aagctgcttt tgacgttaaa
    32701 gtcaaaaacg tgaacatcgt gaacgtacgt gcgaaattca aacgtatggg caaatatgca
    32761 ggatatacaa aaaaacgtcg caaagcgatc gttacattga ctgaagattc aaaagaaatc
    32821 caattattcg aagctgctga ataagcacta atagataact cattaaatta ggagggaaaa
    32881 acgtggcgat taaaaagtac aaacctacct caaatggtcg tcgtaacatg acaggttctg
    32941 attttgctga aatcacaaca tcaacacctg aaaaatcatt gttacaacca ttgaaaaacc
    33001 atgctggacg taacaacaac ggccgcatca ctgtacgtca tcaaggtggc ggacacaaac
    33061 gccaatatcg cgtgatcgac ttcaaacgta acaaagataa cgttgttgca actgttcaaa
    33121 cgatcgaata tgatccaaac cgttcagcta acatcgcgtt agtacactat gaagatggag
    33181 tgaaagctta catcttagca cctaaaggac tagaagtggg tatgaaactt gtttcaggtc
    33241 cagaagcaga tatcaaagtc ggaaatgctc ttccattaga aaacattcca gttggtactg
    33301 ttatccacaa cattgaaatg aaacctggta aaggtggaca attgatccgt tctgctggta
    33361 caagtgctca agtacttggt aaagaaggca aatacgtatt gatccgcttg aactctggtg
    33421 aagttcgtat gatcttagct gcatgccgtg ctacaatcgg ttctgtaggt aacgaacaac
    33481 acgaattaat caacatcggt aaagctggac gctctcgttg gatgcgtaaa cgcccaactg
    33541 tacgtggtag cgtaatgaac cctaatgatc acccacacgg tggtggtgaa ggtaaagctc
    33601 ctatcggacg taaagcacca gtttctcctt ggggtcaacc agctcttggt tacaaaactc
    33661 gtaacaaaaa agctaaatca gacaaactta ttgttcgtcg tcgcaaaaat aaataattac
    33721 tttgaccatg tgtcgcacat tttgagagga ggttcaccat gggtcgtagt ttaaagaaag
    33781 gaccttttgt cgatgagcat ttaatgaaaa aagttgaagc tcaagcaggg gttgaaaaga
    33841 aaaaagttat caaaacttgg tcacgccgtt caacagtttt cccaagtttt gttggatata
    33901 ccatcgcagt ttatgatgga cgtaaacatg taccagttta catccaagaa gacatggtag
    33961 gacacaaatt aggtgaattt gcacctacaa gaacttatcg tggacatgct gctgacgata
    34021 agaaaacgaa acgctaattt gaggggagga taaatccaaa tggcagaaca aatcacatca
    34081 gctaaagcaa ctgcaaaaac agttcgcact tcacctcgaa aagcccgttt agtaattgac
    34141 cttatcagag gtaaaagtgt cgcagatgca atttcaatct tgaaattcac accaaacaaa
    34201 tctgctggaa tcattgaaaa agtattgatg tcagcagttg cgaatgcaga aaataacttt
    34261 gacttagatg ttgaaaactt ggtagtaagc gaagcttttg ttaacgaagg acctacaatg
    34321 aaacgtttcc gtcctcgtgc aaaaggatca gcttctccaa tcaacaaacg tacaagtcat
    34381 atcacggtag tcgtatcaga aaaataagga gggataatca gtgggtcaaa aagtacatcc
    34441 aattggaatg cgtgtaggca tcatccgcga ctgggatgct aaatggtatg ctgaaaaaga
    34501 gtatgctgag ttcttacacg aagatttaag aatccgtaaa tttatttcaa ctaaacttgc
    34561 tgatgctgct gtatcaacag ttgaaatcga acgcgcaaca ggtcgcgtaa acatttcaat
    34621 tcacacagct aaaccaggta tggttattgg taaaggcggt tctgaagtcg aaagcctaag
    34681 aaaagaatta aacaaattaa ctggtaaaag aatccatatc aatatcgtgg aaatcaaaaa
    34741 accagatcta gatgcaaaat tagtaggtga aggaatcgca cgtcaattag aaaaccgtgt
    34801 tgctttccgt cgcgctcaaa aacaagccat ccaacgctca atgcgtgctg gcgctcaagg
    34861 gatcaaaact caagtatctg gtcgtctaaa cggagcagat atcgctcgtt cagaaggata
    34921 ctcagaagga actgttcctc ttcatacttt acgtgcggac attgattacg catgggaaga
    34981 agcagataca acatacggaa aactaggagt taaagtgtgg atctatcgtg gagaaattct
    35041 tccaactaaa aaaaacactg agaaaggagg gaaataatca tgttagtacc taaacgtgta
    35101 aaacaccgtc gtgaattccg cggaaaaatg cgcggggaag ctaaaggcgg aaaagaagta
    35161 gcattcggtg aatacggttt gcaagctgtt gattcacatt ggatcacaaa ccgccaaatc
    35221 gaagctgctc gtatcgcaat gactcgttac atgaaacgtg gtgggaaagt atggattaaa
    35281 attttccctc acaaatctta tactgccaaa gcaattgggg tacgtatggg ttctggtaaa
    35341 ggggcacctg aaggatgggt tgcaccagta aaacgtggta aaatcatgtt tgaaatcgca
    35401 ggcgttcctg aagaagtagc tcgtgaagcg ttacgtctag cttctcacaa attaccaatg
    35461 aaaactaaga tcgtaaaacg tgaggaaatg ggtggtgaat cgaatgaagg ttaaagaaat
    35521 cagagaatta accactgccg aaatgcttga tcaagaaaaa caattaaaag aagaattgtt
    35581 taatcttaga ttccaattag caacaggtca attagaaaac actgcacgta ttaaagaagt
    35641 acgtaaatcg attgcacgca tcaaaactgt gttgcgtgaa caagctaagt aattatggaa
    35701 ggaggccatt aagcgtatga ctgaaggaag aaatcaacgc aaagtttatc aaggtcgcgt
    35761 ggtttcagac aaaatggata aaacaattac cgttgctgtc gaaacaaaga aaaaccaccc
    35821 tatctatggc aaacgcatga actattctaa gaaatacaaa gtgcatgatg aaaacaacac
    35881 agcaaaagtt ggcgacatcg tgagaattat ggaaactcgt ccattatcag ctacaaaacg
    35941 tttccgtttg ttggaagtag tcgaagaagc agttattatc taataacacg agattttcct
    36001 atatagattg aatgcaaaat ctgaaaggag gatacacaac gtgatccaag cagaaagtcg
    36061 tttaaaagtc gctgataact caggcgcacg tgaaattttg acgatcaaag tccttggtgg
    36121 atctggtcgt aaaactgcta acatcggtga tgtgatcgtt gctacggtta aacaagcaac
    36181 gccaggtggg gttgtcaaaa aaggtgacgt cgttaaagcc gttatcgttc gaacaaaatc
    36241 aggagctcgt cgtacagacg gttcatatat taaatttgat gaaaatgctg cagtgattat
    36301 tcgtgatgat aaaagccctc gtggaacacg tatctttggg ccagttgcac gtgaactacg
    36361 tgaaaacaac ttcatgaaga tcgtttcact agcaccagaa gttttataat cttgagacac
    36421 ttatcaaagg aggtgcgaaa cagttatgtt tgttaaaaaa ggcgataaag ttaaagttat
    36481 cactggaaaa gacaaaaaca aagaaggcgt tgtactagca gcgttcccga aaaaagataa
    36541 agttattgtc gaaggtgtaa atgtcgttaa aaaacaccaa aaaccttctc aagctgctcc
    36601 tcaaggtgga attgttgaga tggaagcgcc aattcatgtt tctaacgtga tggtggttga
    36661 ctctactggt gtagctggcc gagttggcta caaagaagta gacggcaaaa aagtccgtgt
    36721 ttctaaaaaa accggtgaag ttttagataa ataaaagttt aaggaaggag ggtcttactg
    36781 aatgaaccgc ctaaaagaaa aatatcttaa agaagtaact ccatcattga tggaaaaatt
    36841 taactatagc tctgttatgc aaacacctaa agttgaaaaa atcgtcatca acatgggtgt
    36901 tggtgatgcg gtatcaaacg ctaaaaactt agataaagca gttgaagaat taactttgat
    36961 cactggtcaa aaaccaatga ttacaaaagc taaaaaatca atcgctggat tccgtttacg
    37021 tgaaggaatg ccaattggtg caaaagttac attgcgcgga gaaagaatgt acgaattctt
    37081 agacaaattg gtttctgttt cactaccacg tgtacgtgac ttccatggtg taagtaaaaa
    37141 agcctttgac ggtcgtggaa actatacttt aggtattaaa gaacaattga tcttcccaga
    37201 agtagattat gatttagtag ataaagtacg tggtatggac atcgttattg taacaacagc
    37261 aaactctgat gaagaatctc gtgagttgtt gacacaatta ggcatgccat tccaaaaata
    37321 attaaaggag gcgaactacg tggctaaaaa atcaatgatt gctaaaaaca aacgtcctgc
    37381 aaaattttca acacaagctt atactcgttg cgaacgttgc ggacgtccac attcagttta
    37441 tcgtaagttc catctttgcc gtatttgctt ccgcgaactt gcctataaag gtcaaattcc
    37501 cggcgtgaag aaagctagct ggtaaaaagt aaattcgcat aagaaggagg taaaagatca
    37561 atggtcatga cagatccaat tgcagatttt ctaactcgta tccgtaacgc aaacatggtt
    37621 aaacatgagt ctttagaagt acctgcatca aaaattaaac gtgacatcgc tgaaatcttg
    37681 aaacgtgaag gtttcgttcg tgatgttgaa tacatcgaag atgacaaaca aggcgtgatc
    37741 cgtgttttcc taaaatttgg taaaaacggc gaacgtgtta tcacaaactt aaaacgtatc
    37801 tctaaaccag gtttgcgtgc ttatgtcaaa gctgacgaag taccaaaagt attgaacggt
    37861 ttaggtatcg caatcatttc gacttctgaa ggtgttgtca ctgataaaga agctcgtgaa
    37921 aaaaatatcg gcggcgaagt tatcgcttac gtatggtaat caagtagaat ctacaaggag
    37981 gtgtctttaa gtgagccgta tcggaaataa agtcgttgtt atccctgaag gagtaacagt
    38041 tactcaagac ggaaacaacg ttacagttaa aggaccaaaa ggtgaattga ctcgtacttt
    38101 ttcagcagat atcaaaatga atatcgaagg aaacgaagta actttcactc gtccaaacga
    38161 cagtaaagaa atgaaaacaa ttcatggaac aactcgtgcg aacttcaaca acatggttgt
    38221 aggtgtcagc gaaggtttcc aaaaagcgtt agaactaatc ggggttgggt accgtgcaca
    38281 attacaagga tcaaaacttg tattgaacgt tggttactca catccagtag aaatcacacc
    38341 accagctgga gtaactgttg aagtaccagc taacacacaa gtaattgtta aaggtgcgaa
    38401 caaagaagaa gttggcgaat tagctgcaaa cattcgtgga actcgtcctc cagaacctta
    38461 taaaggtaaa ggtattcgtt atgttggcga atatgtacgc cgtaaagaag gtaaaactgg
    38521 taaataatcg tttagaaaaa ttgcatagaa gccagcagca ggaaaaaggc atttggctga
    38581 agtttacctt caaaaaaata catttttact tgtgccgcag cttctatttt tcgaaacgta
    38641 tttactgctt gaaatagtag tacaataaaa ataaagaggt gacaattgtg attacaaaac
    38701 cagataagaa caaaacacgt caaaagagac atcgtcgtgt acgtaacaat atctctggta
    38761 ctgctgagcg cccacgcttg aacgtttttc gttctaacaa aaacatctac gctcaaatta
    38821 ttgatgacgt agcgggtgta acgctagcaa gtgcctctac cttggataaa gaaatttcag
    38881 gtggaacaaa aacagaagca gcacaagctg tcggtaaact agttgctgaa cgtgcagctg
    38941 aaaaaggcat taaagtagta gtctttgacc gtggtggata cctttaccat ggccgtgtgc
    39001 aagctttagc tgaagctgct cgcgaaaatg gactagaatt ttagaagaag gaggaatacc
    39061 attcatggtt tatatcgatc caaaacattt ggaattagaa gaccgcgttg ttgcaattaa
    39121 ccgtgtaaca aaagttgtta aaggtggacg tcgtctacgt tttgctgctt tagttgttgt
    39181 cggtgacaaa aacggacacg tagggtttgg tacaggtaaa gctcaagaag ttcctgaagc
    39241 aatccgtaaa gcaattgaag atgcgaagaa aaacttagtt gaagtaccta tggttggttc
    39301 aacaatccca cacgaagtaa tcggtgcttt tagtggtggt agaatcctaa tgaaacctgc
    39361 tgtagaaggt tctggggttg ctgctggtgg accagttcgt gccgtattag aattagctgg
    39421 ggtagcagat atcacttcta aatctttagg ctcaaataca ccaatcaacg ttgttcgcgc
    39481 aacagttgaa ggtttgaaac aattgaaacg tgcagaagaa gttgcagaac ttcgcggcaa
    39541 atcagttgaa gaattaatcg gataaggagg acaacaataa tggctgaatt aaaagttact
    39601 ttaaaacgca gtattatcgg acgtcctcaa aaccaacgcg atacagttaa agcgttaggt
    39661 cttacaaaga ttaacagttc agtggttaag ccttctaatg aagcaattaa aggcatgatc
    39721 aatactgttt ctcacctagt ggacgttgaa gaaatttaat ttagaaaaat cataactaga
    39781 acattaagga ggtgccaaac ctatgaaact tcatgaatta aaacctgctg aaggttcacg
    39841 ccaagtacgt aaccgtgttg gtcgtggtac ttcatctggt aacggaaaaa ctgccggacg
    39901 cggacaaaaa ggacaaaaag ctcgttcagg cggcggtgtg cgtttaggat ttgaaggggg
    39961 acaaacacca ttgttccgtc gtttaccaaa acgaggattc acaaacgtga accgtaaaga
    40021 ctatgcagtc attaatcttg atgtattgaa ccgctttgaa gacggagctg aagtaactcc
    40081 tgttgcttta gttgaagctg gaatcgtgaa aaacgaaaaa gctggaatca aagtattagc
    40141 taatggggaa ctaaacaaga aattaactgt gaaagcagca aaattctcta aagcagctca
    40201 agaagcaatt gaagcggctg gtggatcaat cgaggtgatc taatgtttag actattaaaa
    40261 gatgctttta aagtaaaaga cattagatcg aaaatcctat ttactgtatt tgtgttgttt
    40321 gtatttcgcc taggtgcaca tattactgta ccaggcgtaa atgctgcttc attgcaaaac
    40381 ctttcttctt tgccattttt caacatgttg aacttggtaa gtggtagtgc gatgcaaaac
    40441 ttttccattt tctcaatggg ggtttcacca tacattactg cttctatcgt cattcagctt
    40501 cttcaaatgg acatcgtgcc tcgatttgtt gaatggtcga aacaagggga agttggtcgt
    40561 aaaaaactga atcaagcgac tcgatatctg acattagtat tggcatttat ccaatctgtc
    40621 ggtatcactg ctggattcaa tcaatacgct caattaggtt tcgtgaataa tcctaacgta
    40681 gcaacttacg taacgatcgg tttgattcta acagccggta cgatgtttgt tacttggcta
    40741 ggagaacaga tcacagaaaa aggtatcgga aatggcgtat caatgatcat ttttgcaggt
    40801 atcatttcta gattgcctga aagtatcaga gaaatttatg aagattactt tataaatatt
    40861 gatccttcag acatatggaa atctgctatt tttgtggcca ttttactagt agcggtcttg
    40921 ttgatcgtga cattcgtaac atttttccaa caagctgaac gcaagatccc gatccaatat
    40981 acgaaacgcg tagctggcgc gcctacaagc agctatctgc cgttgaaagt aaatgcagct
    41041 ggagttattc cagttatctt tgctagttcg tttattgcta cgccaaatgc tgttttacaa
    41101 gcattgagcg gtagattcgg ttctgaaaac tggtacaaag tgatgatgga gatctttaac
    41161 tataatacag cacctggtgc tacgatttat acgattctga tcgttgcctt cactttcttc
    41221 tacgcctttg ttcaagtaaa ccctgagaaa ttagcggaaa acttgcaaaa gcaaggaagt
    41281 tatataccaa gcgtacgccc tggtaagggg actgaagagt atgtctcaag attactgatg
    41341 cgtttaagta cagttggtgc agtcttcctt ggtttagtgg cattgcttcc aatcattgct
    41401 caaatggttt gggatcttcc acaatcaatc ggattaggcg gtacaagctt actgatcgtt
    41461 atcggtgtag ctttagaaac tgcgaaacag ttagaaggct taatgctgaa acgtaaatat
    41521 gttggattca ttgagtaaga aagcgtgttg aggtttcccc tcaacacacg ttctcacaca
    41581 tttattagga gggaaaacaa tgaacctcat tttaatgggg ctgccaggcg caggtaaagg
    41641 aacacaagct gaaaaaatca ttgatgctta tggtattccg cacatctcaa caggagatat
    41701 gttccgcgca gcgatgcaaa acgaaacagc tcttggcctg gaagcaaagt cttatatgga
    41761 taaaggtgca ttagttcctg atgaagtaac aaatggaatc gtgaaagagc gactagctga
    41821 accagataca gagaaaggct tcttattaga tggctttcca cgtacgctag atcaagcaaa
    41881 agctctggac gcaatgctaa aagatttgaa taaaaaaatt gatgctgtca tcgatatcca
    41941 tgttggtgaa gaaatcttgg tcgaacgtct agccggccga tttatctgca gcaattgtgg
    42001 agcaacttac cataaaattt tcaaccctac aaaagttgaa gacacatgtg atcgttgcgg
    42061 tgggcatgaa ttctatcaaa gagaagatga taaacctgag acggttaaaa atcgtctggc
    42121 tatcaacatc aaaaatagtg aaccaatctt ggcttattat aaagaacaag gtttgctaaa
    42181 cactattgat ggtgctcgtg aaatcgatgc tgtatttgca gatgtacaaa aaatcattga
    42241 aaaatagcac gattgcccgt agccgacttg cattatgcag ttactcttgt caagttcaat
    42301 aaattatgat agaatatgtc agtccgactt ctagagaatc ctctagatga tccattttga
    42361 gaattttgag gtgggaactg cagtttccgc cattttatcg tgtgaaaatg cgaaacaagt
    42421 acaaggaggt actgtgcgtg gcaaaagaag atatgattga agtcgaaggt acagtcgtcg
    42481 aaactttgcc gaatgcaatg tttaaagttg aactagaaaa cggacaccaa gttcttgcta
    42541 ctgtttcagg taaaattcgt atgcattaca tccgtatctt acctggtgac aaagtaacgg
    42601 ttgaattgtc tccatacgat cttaaccgtg gccgtattac ttatcgcttt aaataattgt
    42661 actccgtaaa actacaggag gtattatcat gaaagtaaga ccatcagtaa aaccaatgtg
    42721 cgaacattgt aaagtaattc gtcgtaaagg acgcgttatg gtgatttgcc cagcaaatcc
    42781 aaaacataaa caacgtcaag gataattgga ggtgctataa atatggctcg tattgcagga
    42841 gtagatattc ctcgtgataa acgtgtagta gtttcactta cttatatcta tggtattggt
    42901 aacactactg ctaaaaagat cttagctgat gctggtgtat ctgaagatgt tcgcgttcgt
    42961 gaattaacga atgaacaaac agatgctatc cgtgcagaag tagataaatt aaaagttgaa
    43021 ggggatcttc gtcgtgaagt gaacttgaac atcaaacgtt tgatggaaat cggttcttac
    43081 cgtggaatcc gtcatcgccg tggattgcct gttcgtggac aaaacacgaa aaacaatgca
    43141 cgtactcgta aaggcccagc tcgcgctatc gctggcaaga aaaaataatt cttaagtgaa
    43201 ggaggttaaa acttcatggc agcaaaaaaa gtgaatcgta aacgccgtgt gaagaagaat
    43261 atagaagcag gtattgcaca tatccattct acattcaata acactatcat catgatcact
    43321 gatactcacg gaaatgcatt agcgtggtca tcagctggtt cattaggttt caaaggaagc
    43381 aaaaaatcaa ctccttttgc cgctcaaatg gcagcagaaa ctgctacaaa agcagcaatg
    43441 gaacacggat taaagactgt tgaagtaaca gttaaaggac ctggttctgg acgtgaagca
    43501 gcaattcgtt cattacaagc tacaggttta gaagtgactg caattcgtga cgtgactcca
    43561 gttcctcata atggtgcccg ccctccaaaa cgccgtcgtg tttaatgagt gccgctcatt
    43621 ttgagtttta tcagtaacgt cattgaacaa gacacttttc gttttgaaag gggtaaagat
    43681 aagaatgatt gagttcgaaa aaccaagaat cgcaaaaatt gatgaagaaa aagattatgg
    43741 caagttcatc gttgaacctc ttgaaagagg gtatgggact actttaggga attccctacg
    43801 tcgtatttta ttatcttctt tgccaggtgc tgccatcact aatattcaaa tcgatggtgt
    43861 actgcatgaa ttctctacca tcaaaggtgt tagagaagac gtcactcaaa tcatcttgaa
    43921 cattaaaggc ttagcgctga aaatgtatgg ccaagaagaa aaaacccttg aaatcgatat
    43981 taccggacct gctacagtaa ctgctggcga tattatcgtt gatagtgatg tagaaattct
    44041 taacaaagat atgtacatct gtagtgtctc tgaaggagct acgttccatg ctcgcttaac
    44101 agtgaaacct ggacgtggtt atgttcaagc agatgaaaat aaaaaagaag atatgccaat
    44161 tggcgttctt ccagtcgatt caatctacac acctgttcgt cgtgtgaact atcaagttga
    44221 gaacacacgt gttggacacc gtgaagattt tgataaattg acgatggaga tttggacaga
    44281 tggttcaatt gaaccgttgg aagcaatgag tttgtctgcg aaaatcatga ctgaacattt
    44341 ggatattttt gtgaacttaa ctgatgaagc aaaaaatgct gaaatcatgg tcgaaaaaga
    44401 agaaacacaa aaagaaaaaa tgttggaaat gacgatcgaa gagttagact tgtctgttcg
    44461 ctcttacaat tgtttgaaac gtgcaggaat caatactgta caagaactta caaataaatc
    44521 tgagcctgaa atgatcaaag tccgtaactt aggacgtaaa tcattagaag aagttaaagc
    44581 aaaattgcac gacttaggtt taggtttacg taaagaagat taaaaccttt tacgaaggag
    44641 ggaaaccaac gtgagttatc gtaaactagg acgcacatct agccaacgta aagcgatgtt
    44701 gcgtgattta acaactgatt tattaatcaa cgaacgtatt gtgactactg aagctcgtgc
    44761 gaaagaagtt cgctcaactg cagaaaaaat gatcacttta ggtaaacgcg gagatttgca
    44821 tgcacgtcgt caagctgcgg ctttcgtacg taatgaagtt gccagcgtgc gtgaagaaaa
    44881 tgaggacatc gtcattgaat cagctttaca aaaactgttc aacgatattg cacctcgtta
    44941 cgcagaacgt caaggcggat acacacgcat tttgaagaca gaaccaagac gcggtgatgg
    45001 tgcaccaatg gttgtcttag aacttgtcta aaatcacact aataacatca ctttatagat
    45061 gattctttta gagtgttatg atgttggaag atatttttcc gagtctagct cagcgctgct
    45121 cctcaagcga atatcgcttg gggagtaagc actcatcata aagtgttttt ttataccttc
    45181 aattacctgt catgacagat atgtttgaag gcataaaaaa acattttaag cgagaaataa
    45241 ttgaaaattt tgtctttttt tgatagtgtt agtaggaact ttaggatgaa gaaggagtaa
    45301 tcatggagcc aattattgaa ttagaaaaga taaattataa ataccagcca gatgatcttc
    45361 gccccgcatt gaaagatgtc tcctttacaa ttgataaagg tgagtggatc gctattattg
    45421 gacacaatgg ttcaggaaaa tcgacacttg ccaaaacgat caatggattg cttctgccag
    45481 aatcagggat cgtgaaagtg ggaaatcaaa tattggatga agaaaatatt tggacgatcc
    45541 gacagatggt tggaatggtt tttcaaaatc cagacaatca gtttgttggt tcgacagtag
    45601 aagatgacgt tgcttttggt cttgaaaatc aagggattcc cagagaagaa atgctagtgc
    45661 gtgtgaaaga tgcacttgag aaagttagaa tggctgagtt tgcttcacgc gagcctgctc
    45721 gtttatcagg agggcaaaaa caacgtgttg ccattgccgg cgtggttgcc ttacgacctg
    45781 atatcattat tttagatgaa gcaaccagta tgctagatcc agaaggtcgt gaagaagtga
    45841 tctctacgat caagaagatc aaagaagaaa gtcaattgac cgttatatct atcactcatg
    45901 atattgatga agcagctaat gccaatcgga tactagtgat gagacaaggc gaacttgtcc
    45961 gtgaaggtac accaaaagaa attttttctg ctggtcctga actgatcgac ttaggtctag
    46021 atctaccatt tcctgaaaaa ctgaagagcg cactgaaaga acgtggggtt gatgtgccca
    46081 gtgaatatat gacagaagaa aggatggtgg attggttatg gacatccgtt ttgaacaagt
    46141 agacttcact tatcagccga atacaccatt tgaacaaaga gccttatttg acatcaatat
    46201 gacgatcaaa gaaaacagtt atactgcatt agtagggcat accggaagcg ggaaatcaac
    46261 cttacttcag catttgaatg cgcttgttaa gccaacaagc ggtacagtcc atattggaga
    46321 acgagatatt cagccggata cggataataa gaatttaaag cccatccgaa aaaaagttgg
    46381 catcgtcttt cagtttccag aagcacagct attcgaagaa acggtagcaa aagacattgc
    46441 tttcggtcct aaaaactttg gtgtcagcga agaagaagca ctagtcctag caaaagaaac
    46501 attagaacaa gttgggctgg atgaaagcta tttggaacgt tcgccatttg aactttcagg
    46561 agggcaaatg cgtagagtag caatcgctgg tgtgcttgcc atgaggccgg aagtacttgt
    46621 attggatgaa ccaacggcag gacttgatcc ccagggtaga aaagaaatga tggagatgtt
    46681 ttggcgtctt cataaggaac agcagatcac tattgtatta gttacgcatt taatggatga
    46741 tgtagccaat tttgcagatt atgtctatgt gttggaaaag ggacgaatcg ttaatagcgg
    46801 ggagccgcaa gaggtctttc aaaacattga atggctaaaa gaaaaacaac taggcgtacc
    46861 tactgccacc gagtttgcag aagaattaat ggctaaaggg atgaactttg ctaccttgcc
    46921 tttgacagct gaagaattag cagacgctat cgtccattat gctggaggaa atgcccatga
    46981 tgaataaatt gatatttggt cgttatatgc ctggagattc cttgatccat cgattggatc
    47041 cccgagctaa attattagcc agtttttatt ttatcggaat cattttttta gcaaataatt
    47101 gggaaacgta ccttttgttg gcatttttca cattactttc tgtcttcttg tcaaaagtag
    47161 atttgaaatt ttttatccga ggcattcgcc cattgatctg gctaatcctc ttcactgtag
    47221 ctttgcaggt cttgtttaca agtggcggag aaacttattg gagttggggt gttttccata
    47281 tcactgaatt tggtgtgatg aacggcctct ttattttctg tcgattcgtg ttgatcattt
    47341 ttatgtcgac tctactcacg cttactacac cgcctttgga attatcggat gcgattgagt
    47401 acttgcttcg tccattgaaa gccgtacggt tcccagtcca cgaaatttca ttgatgctgt
    47461 cgattgctct tcgttttgtg cctacattga tggacgaaac agaaaagatc atgaacgctc
    47521 agcgcgctcg cggtgttgat tttggtgaag gaaacctagt tcagaaaatg aaagcagtca
    47581 ttcctttgct gattccttta tttgtcagca gcttcaaccg tgcggaagat cttgcgactg
    47641 ctatggaagc aagaggctac aaaggtgggg aaggaagaac gaaatatcgt gtattgcatt
    47701 ggcataatag agacacgatg gttatcattg cttttgtatt attaactgtg ctactgatct
    47761 ttttgagagg ataaacgttc agtgggccgc tatctattct tttcttgctg atctgtttgc
    47821 aagaaaggat gcgagcagtg tatgacaaga aatattaggg tctgtggcag actaaggtca
    47881 caggcctctt tcttttatct gaaaagaaag aacgaaaaaa ttttataaaa aaatcattat
    47941 tccagataac cttttaggaa caaaggagag aaaaacatgg tacgttacaa agcaatcatt
    48001 gcatacgacg gaacaaattt taatggattc caaaaacagc caaatgggcg tactgttcaa
    48061 gaagaagtag aaaaaacatt acaaaaaatg gcaaatggaa aagaaatcac ggtctttggt
    48121 tcgggaagga cagatgccgg agtacatgcg attgggcaag taatccattt tgattatcca
    48181 gaggagcgtc cgttagaacg aatgcgtttc gcgctagata cccagtcacc agaggatatt
    48241 gctgtaagac aagtagaaat tgtttcagaa gacttccacg caagatattt ggtcaaagaa
    48301 aagacgtatc aatttcgagt ggatataggg aaaccacgca gtccttttcg acgccattat
    48361 gcaagttatt ttccttaccc tcttgatttg gaaaaaattc agcgggcgct tcctgattta
    48421 ttagggactc acgatttcac ctctttttgt gcatcaggaa gctcgataga ggataaagta
    48481 cgtacgatct acgaggctaa gatggaggta aatgaaacaa aagacgaatt gttgtttaca
    48541 tttaggggaa atggcttctt gtataagatg atacggatct tagtaggaac gcttttgaaa
    48601 attggcaatg gccgattgcc tgaagatagt atcccagata ttattgcgaa aaaagatcgt
    48661 aatgcagcag gacctacagc acatcctgaa ggactttatt tgtatgaagt gaaatatgaa
    48721 taagaggttg tgacagaagt ggtcagcttc aagaaataag aaggaattta cgaaaattac
    48781 ttctcaaaat tttgcaaaat ttcggcttat tttcgaagga gctacttctg gaacaccgtg
    48841 aatcagaggt tgtgaaaaag ctgccctgca tcaagtaata agaacaacca aacaaaaata
    48901 gctcgtcata tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg
    48961 tttaaggaag aggttgtgaa atcagcgttt tgacctgagt attagagaaa caaacggaaa
    49021 aatagttcct catattttgc cgtttgttga gctaataccg aaggtcagct gattgaacac
    49081 cgtttaagaa agaggtcgtg agcctgtcat ttaactctga gtcacaagta acaatttcaa
    49141 aaaacagctt ctcatctttg atgaatatcg tgacttgtgg acgaggcgtt gataagtgaa
    49201 tcatcttttt gttacctatt ttttctgtaa ccaaaaaacg atggaacgtt gttttgattg
    49261 cccttaatga ggacagtcgg aacaggtttg tcatttttat atttaaatgg cacaaatttt
    49321 tctaattatt tgtcatttag cgctgaaaat ttttgctatt tatctaaaaa agtgataact
    49381 acttaagatg aagcagaata tttacgccat acggcataca accgcgaaaa atataaaaaa
    49441 aagaaaacga ttgaacgcgt ttttgcagtc gcaaaggaaa agcatggaat gcgctacatc
    49501 aagtatcgag acttggaaaa caatcaggtg tatacgctga ttgtttttgc tgcaatgaat
    49561 ctgaaaaagt tgtccacttg gcagttggaa catatgtctt catatctttt attatacaaa
    49621 aatagcaaaa gcaccagtct acactcttag taaagaggaa actgatcctt ttgtctacat
    49681 tcttaaagta cttaaactaa gtactttatt ttactcttta ggattgatac agtagcatct
    49741 atccctttat taataatctt ttattttttt aaataaagat gttgtctcca tcttcaggca
    49801 ctaaaacttt ttcggaaatt cctttttctt tattgaaaga ctttaattct tctcttgaca
    49861 gtccccaatg gtttacagct tccatatgaa cggcaataat tgtggaatct ggtattgctt
    49921 tatgcaattc atataaatcg tctttcccca taataagaga ttctccaact aaaaaatgat
    49981 tatctccagc atttgctact atgatttctg gattatactg atcaatagtt tgtttaacag
    50041 cttcatacca aactgtatct cctgtaaagt atagtgtctt ctcagtcgaa tgtttgaaaa
    50101 caataccgca aacattacct acataattca agatttctcc tctgccatgt ttgccttttg
    50161 ttttaaccaa ttgtatagat tcaaaaactg tgtcttctcg tagtacctcc acatttttaa
    50221 aaccatatag tcgaatttca ttcgcatcat tttcatcttg cacaaataat tttatttctt
    50281 taggtaggac ctctttcgct atatcgtccc aatgatctag atgtaaatgt gttacaataa
    50341 cagcatcaat accgttgata atatcttcga ctggcattgg caaatcaact agaggattca
    50401 tttgatcttt tcttggtgat ggaaaaggac ctttcgaaat aaatggtgga agtgttcctt
    50461 tttccgctaa cattggatca attaaaaatt ttttacctcc atagtctaag atagctgttg
    50521 cattacgaat gtgatgtata ttcataaacg ttgtctcctt tttgttgata tgaaatattt
    50581 ttagtataat tcaattccat acaataaata aaccgaacaa aaaaagtctt ttactatttt
    50641 tacttaattt atgaaaggtt gtttaccatg ttagaccaaa cagatataaa tatcattgag
    50701 gaactatcaa aaaatagtcg aatcacaatg aaagaacttg gagaaaaagt acatttgact
    50761 ggacccgctg tctcggcgag agtgacaaaa ctagaggaaa gtggagttat tgaaaattat
    50821 acagtaaaat taaatatgga aaagctaggg ttttcgattc acgctttttt aattataatt
    50881 acgcaaaatt t
//
LOCUS       DADVXU010000010        47884 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.010, whole genome shotgun sequence.
ACCESSION   DADVXU010000010 DADVXU010000000
VERSION     DADVXU010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 47884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.010"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(56..1561)
                     /locus_tag="KB953_000583"
     CDS             complement(56..1561)
                     /locus_tag="KB953_000583"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HBB6865581.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLEFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(1653..2243)
                     /locus_tag="KB953_000584"
     CDS             complement(1653..2243)
                     /locus_tag="KB953_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBB6865582.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            2422..4068
                     /locus_tag="KB953_000585"
     CDS             2422..4068
                     /locus_tag="KB953_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBB6865583.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYFQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            4117..4836
                     /locus_tag="KB953_000586"
     CDS             4117..4836
                     /locus_tag="KB953_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBB6865584.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            5315..6262
                     /locus_tag="KB953_000587"
     CDS             5315..6262
                     /locus_tag="KB953_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBB6865585.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            6274..7194
                     /locus_tag="KB953_000588"
     CDS             6274..7194
                     /locus_tag="KB953_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBB6865586.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            7235..8692
                     /locus_tag="KB953_000589"
     CDS             7235..8692
                     /locus_tag="KB953_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBB6865587.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            9037..9651
                     /locus_tag="KB953_000590"
     CDS             9037..9651
                     /locus_tag="KB953_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBB6865588.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            9644..11368
                     /locus_tag="KB953_000591"
     CDS             9644..11368
                     /locus_tag="KB953_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBB6865589.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            11365..12816
                     /locus_tag="KB953_000592"
     CDS             11365..12816
                     /locus_tag="KB953_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBB6865590.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(13289..15442)
                     /locus_tag="KB953_000593"
     CDS             complement(13289..15442)
                     /locus_tag="KB953_000593"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HBB6865591.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(15444..16490)
                     /locus_tag="KB953_000594"
     CDS             complement(15444..16490)
                     /locus_tag="KB953_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBB6865592.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            16640..17929
                     /locus_tag="KB953_000595"
     CDS             16640..17929
                     /locus_tag="KB953_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HBB6865593.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            17942..20644
                     /locus_tag="KB953_000596"
     CDS             17942..20644
                     /locus_tag="KB953_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBB6865594.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            20629..21486
                     /locus_tag="KB953_000597"
     CDS             20629..21486
                     /locus_tag="KB953_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBB6865595.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(21557..22456)
                     /locus_tag="KB953_000598"
     CDS             complement(21557..22456)
                     /locus_tag="KB953_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBB6865596.1"
                     /translation="MVQLVCDCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            22625..24796
                     /locus_tag="KB953_000599"
     CDS             22625..24796
                     /locus_tag="KB953_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HBB6865597.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            24864..25205
                     /locus_tag="KB953_000600"
     CDS             24864..25205
                     /locus_tag="KB953_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBB6865598.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(25481..27235)
                     /locus_tag="KB953_000601"
     CDS             complement(25481..27235)
                     /locus_tag="KB953_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6865599.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(27246..28082)
                     /locus_tag="KB953_000602"
     CDS             complement(27246..28082)
                     /locus_tag="KB953_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBB6865600.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(28084..28767)
                     /locus_tag="KB953_000603"
     CDS             complement(28084..28767)
                     /locus_tag="KB953_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HBB6865601.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            complement(28926..30320)
                     /gene="uxaC"
                     /locus_tag="KB953_000604"
     CDS             complement(28926..30320)
                     /gene="uxaC"
                     /locus_tag="KB953_000604"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBB6865602.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            complement(30359..31858)
                     /locus_tag="KB953_000605"
     CDS             complement(30359..31858)
                     /locus_tag="KB953_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HBB6865603.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            complement(31851..33272)
                     /locus_tag="KB953_000606"
     CDS             complement(31851..33272)
                     /locus_tag="KB953_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HBB6865604.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            complement(33332..34324)
                     /locus_tag="KB953_000607"
     CDS             complement(33332..34324)
                     /locus_tag="KB953_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBB6865605.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            34556..36754
                     /locus_tag="KB953_000608"
     CDS             34556..36754
                     /locus_tag="KB953_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6865606.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            complement(37212..38084)
                     /locus_tag="KB953_000609"
     CDS             complement(37212..38084)
                     /locus_tag="KB953_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBB6865607.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            38384..39358
                     /locus_tag="KB953_000610"
     CDS             38384..39358
                     /locus_tag="KB953_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBB6865608.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            39380..40072
                     /locus_tag="KB953_000611"
     CDS             39380..40072
                     /locus_tag="KB953_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBB6865609.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            40038..41546
                     /locus_tag="KB953_000612"
     CDS             40038..41546
                     /locus_tag="KB953_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBB6865610.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            41620..42723
                     /locus_tag="KB953_000613"
     CDS             41620..42723
                     /locus_tag="KB953_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBB6865611.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            42726..44063
                     /locus_tag="KB953_000614"
     CDS             42726..44063
                     /locus_tag="KB953_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HBB6865612.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            44050..44928
                     /locus_tag="KB953_000615"
     CDS             44050..44928
                     /locus_tag="KB953_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HBB6865613.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            44925..45848
                     /locus_tag="KB953_000616"
     CDS             44925..45848
                     /locus_tag="KB953_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HBB6865614.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            45845..46255
                     /locus_tag="KB953_000617"
     CDS             45845..46255
                     /locus_tag="KB953_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HBB6865615.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            46259..47827
                     /locus_tag="KB953_000618"
     CDS             46259..47827
                     /locus_tag="KB953_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBB6865616.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
BASE COUNT        15392 a         8538 c         9785 g        14169 t
ORIGIN      
        1 aaatcacagt cccttttttt cttagaaatg ggaaaccact agtatataaa taccattatt
       61 cttcaatcaa ccgcttcacg attgcataat ccccttcata aggttcatct acaccattga
      121 ttttttgata gggatccacg ccttttcccg caataacgac tgaatcttgc ggagcgcttt
      181 ctgctaaagc gcgtcgaatc gcttcaggac gatccatttc ataatgaacg atcaaatctg
      241 gattagtaat tgcttcattg atttcttccg cgattttttt cggatcttca aatgctgggt
      301 catctgccgt caaaaaggcg atgtcagctg tttctgatag cacttttcca aaatcatgtc
      361 gacgggagat tgctttgttt cctgggctgc ctaatacaac aattacacgg ccatctggat
      421 gttcattttt ggcaaattcc agcaatgttt tcaagcttag ataattatgt gcatagtcca
      481 catagacatg cgctccattg gattggatca attgatccat tcggcctggt acacgagctt
      541 cttttaatcc gtcttgtgca tcttgactgg aggcacctgt tagtgctgcg gcaatgatcg
      601 cactggctgc attttcttgg ttgaatcctc cagccagcaa aatatcataa gcagtatcgc
      661 agatagctaa gcgatcttca tgagaagata aagtaaaacc gtgagtacct ttatctgaac
      721 ggatcacttg ataattgctt tcttccgaac gaccataagt gatcgttgga atctggaata
      781 gatcggctgt ttccctaagt aaatcaaaat agtcactttc atgatttaaa acaactgttt
      841 ttgaattcaa gatcaattgt cgcttgcaat agaaatagtc atcaaatgtc ggatgttcga
      901 tcggactgat atggtcaggc gagatattca agaagattcc gacatctaaa gtcaagccgt
      961 atacgcgttg tgttttgtaa gcttgtgaag aaacttccat cactagatga gtcataccat
     1021 tttcaactgc ttctgacatc atacgatata aatctaatga ctcaggcgtt gtcaaatggg
     1081 atttgaaata tgttttgcca tctaacgttg tattcatagt agaaagcaat gctgtttttt
     1141 tatttgttgt gtgatccaag attgcttttg taaagtacgc agcagttgtt ttaccttttg
     1201 ttccagtgaa tccgatcact ttcaatttat tttgcggata gtcgtaaaaa gccatagcaa
     1261 gaagcgccat tgctttacgg atatccgtta cgatgattcc tactgctgaa ttttcgtatg
     1321 gttgttctga tacataatat tgaataccct gttcgattgc tgaatccaaa tattcttctt
     1381 tgaaattcat tcctttacag aaaaacaaag tagaagcatc agcttttctt gaatcataac
     1441 ttaactgaag aaattcagct ttttctggta catcaagatg ccagcctctt gttgaaacaa
     1501 attcttttaa tagattttct tttaatagta aatgacggat gtcttctaaa gagaagctca
     1561 ttgattgatc cctcgcttta ttcaatttct tggtttatta tagcacactc tttttaatat
     1621 atcgtttgtt tccgctatga caaaatccag ctttattcaa attttcttga cacaggggac
     1681 aagcggatga gtattttcat aaacaatgaa ctgactgtaa tagataggag aacgaatagt
     1741 gtctccaaca atccaaaaac ataataattc aaaaagacga taagactatt ggtaagtaaa
     1801 agtccttgcg gcattggaag ccgaaaaaat ttgtggaaaa taatcggcgg tatcgttata
     1861 ccgccatttg aggccccgac tgtgtacaat gtgtaaaccc caagactgaa caaaagactt
     1921 cctgaaatca gtgaaaagaa atgtgaggag atcatttcca cttttggaac aatctctaaa
     1981 acaacaggaa aaagcagact tcccgctaca gtcttaaaaa atactgattt tcctaatagg
     2041 acagcggcta gaagcagcat cataagattg atgctccaaa cagtaactga gcgttggata
     2101 gaaaaggccg tttcaactaa atgtccgatg gcgcccactc cggcggaagc tatatcgtga
     2161 gggagcaaga accaattcaa tccaactgtg ataaagaaaa gacctaaaaa tataacgggt
     2221 agttttttta tttcttgtgt cattgatatc tcctataaat gttagccata aaaggctgta
     2281 tatttcttta ctttaccaaa aaaacgaaac cgaattcaaa aaaacaattg ttcttataaa
     2341 agttatataa aatcgattgc cagtttctgt tttaccaatt aaaatgatag taatgttttg
     2401 tagaaagttg gaatctgacc gatggataat caaagaaaac cagcattaga tcaattaaca
     2461 ccgcaagaaa aaatggcacg aggctctgct tggatgaccg ccagtaatat catttcaaga
     2521 ttgctaggcg ctgtctatat cattccctgg tatgcttgga tgggcgaaaa tgctaaggct
     2581 gccaatggat tattcaatat gggatacaat atatatgcat tgtttctact gatctctaca
     2641 gcaggaatac ctgctgcgat tgcaaaacaa actgcacgct ataattcatt gaatgaatat
     2701 ggcacttcac gacgattatt tatccgtgca ttacagatga tgggcgggct aggcctgttg
     2761 tttgctttgt tcatgtacat cgcctctcct tggctagctc atgcttctgg tggcggagaa
     2821 gaactgattc caacgatgcg ttctttgagt atcgctgtat tggtttttcc ttgtatgagt
     2881 gtgatacgag ggtattttca aggcaatcaa gaaatgatgc cttatgcgct gtcgcagatt
     2941 gtagaacagg tggcacgtat tttttatatg ctattggcta cattcatcat catgaaagtc
     3001 atggatggtg actatgtgac ggcggtcacg caatcaactt tcgcggcatt tatcggtatg
     3061 ctagcaagta ttcttgtcct tttgtatttt ttgaagaaac atcaagcgta tacatcggcg
     3121 tttattcact acagcgaaaa caaaataaat attgcaacga aggaattgtt gttagatacc
     3181 gttaaagaag cgattccgtt tattattgtg ggttcagggg taacgatctt caaattagtc
     3241 gatcagttca cttttatgaa gatcatgtct gcatccaccg agtattccaa tgcacagtta
     3301 ctggatcttt tttcgatttt tagtgccaat ccagataagt tgacgatggt agttattgca
     3361 ttggcaacat cgatcgcgtc tactgggtta ccgttgatta cagaagctgt tacgttgaaa
     3421 gatcgagtag gtctggcaaa attgacaagc ggaaatttac agttgttttc attttttatg
     3481 tttccggcga catttggtgt tatgttactt gcctatccgc taaatacatt attttataca
     3541 ccggattctc taggaagtaa tgtactgatc caagcaagtt ttgtaggact cttcctagga
     3601 ctatatatgc tagtttccaa catgctgcaa ggaatgtttg aaaacaaggc ggcaattaat
     3661 tatttggtta ttgggtttct agtgaaactc cttcttcaat acccagccat tcgtttattt
     3721 gaagtatacg gccctttatt ggcgacaatg atcggatttg ctgtgtcttg taccttgatc
     3781 ttaaagagaa tccatgaagt agcccgcttt aaaccaagtt ttgtctggcg caggacattg
     3841 ctgatcttta ttcttacctt gattatgctt ttggcttcgt ggatcacaaa gatgattttt
     3901 ggtgcttttt taaacgaaga tagcaaattc cagtctctat tgttgattct tgtagtagca
     3961 ggtatagggg ctttagtgta tggttattta gctttgaaaa tccgcttggc agaaagacta
     4021 ctaggaccag gaatagcgcg tttacgacat aaattaaaaa taaaataagt gaatcagaag
     4081 agctagattg atgctctttt gaaatggggg aaacgcatgc gtttagataa gtttttagca
     4141 gatatgggac ttggcagccg caaagaagtc aaaggattga tcaaaaaagg aacgatcgaa
     4201 gtcaatggac aagtggtgaa aagcgataaa gcgcaagtaa atgaattcga agatgcggtt
     4261 acttatttag gagaaatcat tacgtatcaa aaagattttt actacatttt gcataaacct
     4321 gcaggagtca tttctgctac tcaggataat catgatcaaa ccgtaatcga tttattggaa
     4381 gaccaggatt atcgggaaga tctttttcca gtcggccgat tagataaaga cacagagggc
     4441 ctcttgattt taacgaatga cggggtgttt gcccatcagt tactatcacc taaaaaacat
     4501 gtggagaaag aatatctagc tgaggttagc ggtataatga cagaagaaga tatccgttta
     4561 tttgcagaag gactgattat tgatggcgat gagcagacat tgccggcaaa actccaaatt
     4621 gatcaagtag atcaagagac agaaacttca attatccgtc tgattttgca tgaaggaaag
     4681 tttcaccaag ttaagcgaat ggtcaaagca gtaggaaaag aagtcttata tttaaaaaga
     4741 atccgtatgg gaaaattttg gctgcctaaa gatttgaata aagggcaata tcgacccatg
     4801 accaaagaag agctagaaca agtgagagaa aaataattga atggatagct aaaagaataa
     4861 gcagagtagt tgtagcgcca tctgaaaagc agacttgtcc gtctgacttt cggatggctt
     4921 ttttgtgaaa tttcggctta tttccgtaga agctacttct ggaacaccgt ttgttcggaa
     4981 taagggactg tgacaagact tttgttacag tccccattgt tccttgtgac tcagagttag
     5041 atagtaagga cacgatctcc ctaatactca ggccaaaacg ctgaattcac aacctctgat
     5101 aaacggtgtt caatcagctg accctcggta ttagctcaaa aaacggcaaa atatgaggaa
     5161 ctattttccc gtttttttct ctaatactca ggccaaaacg ctgatttcac aacctctcct
     5221 atactttgtt tcattttccc tatactttgt ttatttattc tcattattga aagcgctata
     5281 attacgttgc gaatgaatcg aaaggggttt ttagatgaaa aaacgaaagg ggttcaatca
     5341 tttttttatg aatctatggc gttataaagc acttgtcctg atggctgtac cgggtatgct
     5401 ttggatgatt tttttctttt acatacctgt aatggcgaat gtcgtagcat tcaaaaattt
     5461 tcatatttca gcagatgggt tcttggctag cttgagggaa agtccttggg tgggttggga
     5521 aaatttcaaa tttttatttt cttcggatca agcatttctc atcactaaaa atacaatttt
     5581 atataatgta gcatttatcc ttttgaatct gctgatctca gtcgtatttg caatcattat
     5641 gagcgagttg agaaataaac gactcgttaa agtttatcaa acgatgtcct tactacctta
     5701 ttttctatct tgggtcatta tcagttactt tgtttacgct tttttaagtc ctgacaaagg
     5761 aatattcaat cagtggatca ctggacatgg aggagaagct gtcaattggt ataatgaacc
     5821 gaaatattgg ccgtttatct tggtgttcat tggtacatgg aaagggattg gttataacag
     5881 cattatttac tttgcatctg ttatgggaat cgatccgact tattatgaag ctgctatggt
     5941 tgatggagct agcaaatggc agcagatcaa acatgtgacg ataccacaac ttattccact
     6001 aatgacgatt ttaacgattt tagccgtagg aaacattttc cgagctgatt ttggcctgtt
     6061 ttataacatt cctaggaatt ccggtgcatt atatgatgtg actcaagttt tggatacgta
     6121 tatctacaat ggcctgacat ccacaggaga ttttggtatg acggctgctg caggacttta
     6181 ccaatcagtt gtcggctttg tcttgttgat gatcacaaat acgatcgccc gacgttttga
     6241 tagcgattct gcattattct aaggaggaaa aaaatgaaaa aaaagaaagt ggagagagtc
     6301 caagtccgct ctttcaataa gacggctaat tttttctcta atataatgat tgcactcttt
     6361 gctatttcct gtattttgcc atttatattt gttatcgcca tctcttttac cgatgagtcc
     6421 atattgtcga ctcacgggta cagtttttgg ccagaaaagt tcagtatatt tggttatacg
     6481 tacttgttcg accagatgca ggataaaatt ttccaagcgc ttttcgtcac tgtacttgtt
     6541 actgttttag gtacactgat caatagcacg gctacttctt tatatgctta tgcgatttca
     6601 cggtcaaact ttccattccg tcgttttttt accgtttttt gtttgattac gatgttgttc
     6661 tcaccaggga tggtggccaa ttatcttgtt atgacaaatc ttcttcagtt aaaagatacg
     6721 atttgggcat tgatcttgcc aatggctgtc agtcccttca atattattgt gatgcgcaca
     6781 ttctttaaac gttcggtctc tgattcgatt attgaatcgg cacggatcga tggagctagc
     6841 gaactgcgaa tatttgttca gatcgtttta ccgttagctg ttccaggtat tgcgacaatc
     6901 agtttatttg ccgcattggg ttactggaat gactggttca atgctttact atacatccaa
     6961 gatgataagc tagttccact acagtatctt ttgatgaaaa tccaaagtaa tattcaatat
     7021 ctgacacaaa attcaggagc aggaagccaa ttgacaggcg gattagcctc agttccagga
     7081 gaatctgcca gaatggcgat cgtagtcatc tcgacgttgc ctattgcgat cagctatcca
     7141 tttttccaga agcattttgt caaaggtcta acaatcggcg gagtaaaaga ataaagaaac
     7201 aaaaatataa atcataaaat taggaggaag aaaaatgaag ttatggaaga aactagcctt
     7261 tacaggtgtt tcagcgttgt tgttgggaac attagcagca tgcggaggtt ctggttctaa
     7321 agaccaggca gaagctagca attcggacac tttgcagatg tatcaaatcg gagataaacc
     7381 agataatttc gatcaattga tggaagtagc aaacaaacga atcgaaaaag agatcggtgt
     7441 aaaagtcaat atcaattaca ttggctgggg cgattacgaa aagaaaatga acgtcatcat
     7501 ttcttccggg gaaaattatg atatcgcctt tgcgaacaac tatgtttcca atgcccaaaa
     7561 aggagccttt gctgatttga ctgaattagc gcctaagtac gctgaaaaag cttataatga
     7621 tttagatgaa gcatatatta aaggaaatct ggtcaatgga aaattatatg ctttcccagt
     7681 caatggaaat gtctttgctc aacaagtact tacttttaac aaagctttac tagataaata
     7741 cgatctttca attgatggaa tccaatctta cgctgatgca gaaaaagtct tgaaggaatt
     7801 ccatgaaaaa gaaccaaaca cggcagcttt tgccattgga caaggcttca aagtccaagg
     7861 gaactttgat tatcctttag ggaatacgtt accatttgct attgatctgg atggagatga
     7921 ttcgaaaatc atcaaccaat acgataatga aacgtatatc ggactgttac gtacaatgca
     7981 tagctggtac aaacaaggac tcattccaag tgacgctgca acgagcaata aggattatcc
     8041 actagaagga aatacttggc taatgcgtgg agaaacacaa ggaccgtatg attatggcga
     8101 tacaattttg acaaatgcag caaaacaaga acttgtctca aaagcaatta ctgttccact
     8161 aaaatctaca gctcaagcac aaatggcaaa ttttgtcgta tcaaatactt caaaaaataa
     8221 agaaaaatca gtagaacttc tagggctgct taacagtgat ccagaattat tgaatgggct
     8281 tgtttggggg atcgagggag aagcatggga aaaagtcgat ggttcagatg ataaaatcaa
     8341 attgctagat ggttatcagc caaatacaca tatgtctgct tggaatacag gaaacaacaa
     8401 gattctatat acacaagagt ctattacgga tgacatgatt gccaaacgtg atcaatcgat
     8461 tgctgatgca gaaacttcac cgattttagg attcagcttc aacactgaca gtgtcaaaac
     8521 agaattaagt aatatttcta atgtcatgaa tcaatattta gatggattga atacagggac
     8581 tgttgatcca gatgaaacat taccgaaatt gaaagacgct ttggacaagg ctggctatga
     8641 taaggtattg aaagaaatgc aaaaacaata tgatgagttc cgacaagaat aataatgaat
     8701 aaagacaaag gggttgtgac aaaagacgtg tcacaacctc ttgttccgaa taaacggtgt
     8761 tcaaaagcca gtttctcggt attaagctaa atatatgtaa aaatgtggaa agccatattc
     8821 acaaaatttt tcttactact cgaaactaag cggctatttc acaaactctt ggtaaacggt
     8881 gttcaatcag ctaaccctcg gtattcgctc acaaaacgac aaaatatgag gaactatttt
     8941 tccgtttgtt tctctaataa tcaggtcaag gtgctgattg cccaaccttt ttttgtataa
     9001 tgagaaagta taaacttaat gtttggagga gaagatatgt tagggaaggt tttggaagag
     9061 ttatttatcc ggatctgggt cattataaaa ctaaccttgt atttctggat atatacattt
     9121 gccggcggga tcatctttgg attaggtgcg gcatggaaaa cagtcaatga actattttat
     9181 ttgtacggat tcgagtataa agaaatcaca ataaaaagag gctggaatat atataaaaga
     9241 aattttttaa gaggcaatct gctatttagt ttatttttat ctggtactgc tttattgagt
     9301 tacaatttat atttatctgt tcaaatcaaa gggctcattt ttttagctat tgattttatt
     9361 ttactttttg ccttgctctg cctatttgct acttatcaat acagtatgat tttagatagt
     9421 gaatatacga tctctgttcc taatctgcta aagctaagtt ttatttcggt tttttccagc
     9481 ttttcgtctt ttctaaaaat aattattggt agcggtataa ttttaggcgt gacttggcaa
     9541 ttcaaaggat tgatcctgtt tggtgtcatt ggtcttttga cggtttggaa tggtacgatg
     9601 acaacacatt ggcgtgaaga attagataaa cagttggaaa gttatgaata agtttcggat
     9661 tctattttca aaaggaacct tactaaatcg tctgttgcgg aaatacgcgg ttatcatgat
     9721 cggtgtaacg atgactgcta ccgttatttt tagtatccat acttgggaac aaaatcaaaa
     9781 acaggcagaa aacatgacaa gtgatgccgt tcaaagcaca agcagaatgc tgaatgataa
     9841 aacaaccttg agtaggatca tcaaaaacca gcttgtaggg aattccgaaa agatcgaaaa
     9901 tgtgacgacc tacttaacaa aacccataga tcagtatctg atgtatgtat acgaacagca
     9961 aaacagtacc gatgaattag tttctttccc aaatcaaatc aaagatctct acgcaaatta
    10021 tgaagaacta agtgcgatat atatcgtgtt gaatcaacta ccggagtatt atgaatcttc
    10081 tagagaaaac aaaggtgggg aaataaaaaa ggggacacca aaattactag acgcctttta
    10141 tatcaagttg cctatcacac aaggaggagc tgagtcagga agtattttta ttggttttca
    10201 aaagaaagaa ctagatatga ttttagaaaa tctaacgtca ttcaatggac tctctttata
    10261 catgatttct ggaacgacaa atcgactata cacattccat gataataaaa tagctaaaga
    10321 aacgtttgaa agacaagaaa tgatcatcac agagagtctg aagaaaaatt cacaattgcc
    10381 gattgacgat ctggaaaaaa acaattttgt tcaacaccaa gaactgtctg atgattatcg
    10441 tatattagct gtactagata aaaaaagtgt aaaaatcgaa acgggaaaaa atcttgcccc
    10501 gctgatcatt ggtatcatta ttttagacaa tatactgctt atctttcttt ataaaacatt
    10561 caaacgatat tcacagcaag tatctatcgt catgcaggcc atggatcaaa cagcagcagg
    10621 gaatttagag acgcggatcg acataacaaa cacggaatat gaactgaagg aactttcaat
    10681 cggtatcaac gaaatgttgg atagcatcaa tcagtttgta gaagacatct acaaattaga
    10741 aatcaaacag caagacgcac atatgcgggc gcttcaagca cagatcaatc cacacttttt
    10801 atataataca ttagaatata tccgaatgta tgcaatcagc gaaggaagtg aagaattagc
    10861 agatgttgtg tatgcgtttt ccgctttgct tcgaaacaat acgaatcaag agaaaacgat
    10921 cactttaaaa gaagaactgg acttttgcga aaaatatgtc tatttgtatc aaatgcgtta
    10981 tcctaatcga gtagcctatc attttatgat cgatcctgat ttggaaaaga tcgaagtacc
    11041 taaatttgtg atccagccat tagttgaaaa ttattttaaa cacggtattg attttactcg
    11101 attcgataat gcgctaagtg tcaaagtctt gcaggaaggc aaacgggtaa ggatcatcat
    11161 caaagataat ggaaaaggga tgactgaaaa acggctaaaa cagattgaag aaaaactgtc
    11221 tcatcctaaa gtggaacttc acggttctat cggtctgcaa aacgtgaatg aacgtttacg
    11281 tgccagcttc ggcagcagtt attatatgtc gcttgagaat aatgagacag gtggactgac
    11341 cgtttctatt acattcaagg agggatgagc gtgtatcaag tgttattggt agatgatgaa
    11401 tacatgattt tgaatgggtt aaaaaagata attgattggc aaagtttagg ctttcaaata
    11461 gtagcaacag cagagaatgc caaagaagga ctagctgtac ttgaacagcg acagattgat
    11521 ttagtagtaa cggatgtgac aatgccagaa ataaatggac tggaatttat tgaagcggct
    11581 caaaaagaac gtcataattt tgaatttatg attttatcag gataccaaga attcgattat
    11641 ttgaaaggag gtatgcaatt aggcgccgtg aattatttga tgaaacccgt gaataagttc
    11701 gaattggttg aaagcctcaa gaaaatcaaa acacggttag atcagcaaaa tgaacaaaaa
    11761 aatcaacaag aaatctatca agaactgtta ttcagtcaat ggttgaatga agaattggat
    11821 gaaataagtg aagaagaaat aataggacaa ataagtgaga agcagcatag gatagtattg
    11881 attcaactgg caagaactca tgatactctt atcaatagat ggttgcaaga acggcaacaa
    11941 atcttttact atcaacgaaa ttacggagac cgtgtattga tcacgttgtt gttggaggca
    12001 gatgaagttg atggcttctg ttgttttgtt caagaaaact ttcctgatca ggaatggctg
    12061 atcagtatcg gggaagagac agattctttg gacaaaatcc ctgaaacatt ccaacaagcg
    12121 aaagacaatc tacagttgca tcaattttat ggagataaag acgagcatgt tttttacgca
    12181 gtacaagcag aaacaaaaga tcaaaccatc gatttttcaa gtttcagacg cgtgctgcaa
    12241 aataaacaat tggaagtcgc tcaaaaaatg atcactgatt tttttgaaca gtttcagtta
    12301 gcagccatga tgccggaaga tatacgatat tccagttttt tactgtttat ggagattcaa
    12361 cgggaattga ttgatctgga agatgaggaa tatttacaag gaatcgaaaa aatccatcag
    12421 gcaaaaacgg tgcaagaact tcagcagcta cttctgtcat tcatcaaaaa gcatcagaga
    12481 cagaaaaagt acagtgacaa tgtggaaaaa gtaatcgtaa tcctgcacca gcattatcaa
    12541 gaaccactta cactcaaaga agtgtcagaa aacctgcatt taaatgtcat gtatctagga
    12601 cagctgttca aaaaagaaac gaaaaaaagt ttttcagctt atttgaacca tttacgtatg
    12661 gaaaaagcga aacaactgct tttgcacagt aatcaaaaca tcaatgagat agcaagtgag
    12721 attggctaca acaacaccac gtatttctct aaattattca agaaaatcgt cggcagatcc
    12781 cctaaagagt accgagaaaa cagtggagaa tcataaaaaa aaaagccaat gtaatgaaat
    12841 aacttataaa aaattgtcta gaaatctaat tttgggctgt tcttattact tgaggcaagg
    12901 cagctttttc atgacctctc gataaacgga gttcaaaagc cagtttctcg gtattaagcc
    12961 aaatttatgc aaaaatgtgg aaagccattt tcacaaaatt tttcttaata ctcgaaacta
    13021 aacagctttt tcacgacctc tccactaaat ggtgttcaat cagctgaccc tcggtattag
    13081 ctcaaaaaat ggcaaaatat gaggaactat tttgccattt gtctctctaa tactcaggtc
    13141 agaacgctga tttcacaacc tctcaataaa cggtgttcaa aagcttgcat ctgcggtaat
    13201 aagaccaacc aaacgaaaaa tatgaggatc tatttttgct tggctgttct tattacttga
    13261 tgcaggacag ctttttcaca acctcttgtt aattatagtt tgataaggaa aaaggcaaat
    13321 cttttgcttg atatttctta gggtgaggga tgatccctaa ttggtaagag atcgtgccgc
    13381 ctttcagcaa atcttgatgg gtaaaatata atttcgtata ttctctgtcc atccgtttga
    13441 ttttatggat aaattgttgc tgcggccgat taggcgttgc ttcaatcgtc aattcctttc
    13501 catttgacaa aagcaaaaca gccttgtcaa ttaatggcat accgataaca tattcaccgg
    13561 ttccaggcgt gacaggataa aatcccagac tattgaagat ataccaagcg gccatacttc
    13621 cattgtcctc atctccaggg taacccattg gagaatcgtc gaaggtctga gtcatcaact
    13681 gtttaagcag tggctgtgcc atttcaggtt gtcctatata actgaacaag taaggatagt
    13741 gaaaacttgg ctggtttgaa atagctaatt gtccaaattc tatcgcggcc atctcactca
    13801 tttcgtggat ctcaaaacca tatcctcctg ggtcaaacgt tgggggctga ttgcacagct
    13861 caatgagttt ttctttatat ctctcactgc caccatattt tccgatcagt cctgaaaaat
    13921 cgtgataaac agcaaagctg ctttgccaag cacttccttc tgcataatct tgtccccaac
    13981 gattagctga aaatggttct ctgaacttgc catctttatc ttttgaacgc ataaatcctg
    14041 actcatagtc aaagatattt tgatagttca atgattgctg ctgatatttc ttcgtcattt
    14101 cctcttcttc tagtaccttg gcaacttggc taatacaaaa atcgctataa gcatagtcta
    14161 atgtgtgatt gactgattcg tggtagtgat taggaacata gccatatgtc aagtaatctt
    14221 ctgttccttg gcgtccataa tttggatgtt tgctttgaac cgtagcacct ttttccattg
    14281 ccgttagaaa ttcaggcatc aagtctcgtc ggatatcttt tacagcagca tctgcaatga
    14341 ccgcatcgat caatgtccca ggcataagtc cacgttcatc aggagacagc cattttggca
    14401 aatagccact ttctctatag ctgttcaaaa aaccttccag catttcttcg tatttttcta
    14461 cagcaatcaa agaataaaga ggataaaccg ttttatacgt atcccaaaac ccattatttg
    14521 tatagagaac accaggtttt atagatcgac tagtcgtatc atagtggatt ttttgcccac
    14581 taggatcaat ttcataaaat gtttgtggaa ataaaaacac ccgatataaa ttatgataaa
    14641 acgtccgcaa ttgttgttga tcatggtgtg tgagctgtat acgatcaaaa taggactgcc
    14701 attgttcttg actgtgagca aggtaagtct ctttgctttg gttttgttct agttccaggt
    14761 tataagctgc ttgttccgag ctgataaaag atgtcccaaa tcggatagtt tgtttttttg
    14821 tctgttcaaa atacaacggg atcgagtcgc tatttttgtt ggaaacagct aactcatcaa
    14881 ttgttaaagg atattcaaat ctcaatataa aataaaaaga aaaatcagga tcttctgaac
    14941 cagcaaaatg tgtgattttt ccttccactg tgtaaggatc tataacaaag agctgatggc
    15001 gtcccggaaa ggataaaagt aaaccgctgt catttttgct gtaatcaata gataagattc
    15061 cgccatacat gctaggtatc aatgtagaac agatgccata acgtaattga cagatcgaca
    15121 gatgagtcgg gcaaaatata ctttcttctg ggcggtaaga actttgagca tggaaaaggg
    15181 tgttctcctg tagttttcca ttgatcggcg taaacagcat atgactgaaa tcacccatcc
    15241 aaggacttgg ttgatgggta agacggtagc cttgaaaaac gcggtcatca ggatgaaacc
    15301 accagctgcc gttttgatcc gtagtttgcg gtgcaaaata attcatccca aaaggaacgc
    15361 cagtataagg ctggcagttc ccattagaga aatttggctg attcgctgtt gcatgtctag
    15421 tatctatttg accgatattc attttatttc tcctttgttg attgtctttt gatgaatgaa
    15481 actggaaatt tactatcgga tggtcgatct tttgtctcta accaagtgac caaagcatct
    15541 cccgcaagct tacctaactg ttcaaaatct tgtgcgatgg ttgtcagtgg cgggtcaaca
    15601 agagctgctg cttggatatc atcaaatccg ataattgaaa aatcttcagg gatatgataa
    15661 ccatgctgtc tcaagagatt catggtctgg atagcgacaa catcgttttc acaaacaaaa
    15721 gcagttactt catacttagt gatataagaa agcaattcat ctaatgtcgc gcttttgtca
    15781 tccagtgaag tgtggaaagt caaaccagct ttgtccaatg cttcaaggta accaaggtaa
    15841 cgctgtcgaa cggattgtgg atgagtctgc gtaccaaaca aataagcgat ttttgaatgt
    15901 ccctgttcga tcaaataaga ggcagcaagc gcaccgcctt ggaaattatc ggaaagaacg
    15961 gtaggaaaag gtaactcata gattttttta tctaatatga cgacagggaa ttctttcaat
    16021 gaaagctcaa aaagtaagtc aagatgttgt tctgtatcaa gagcataata aatcaatccg
    16081 tcaaattcat tgattagctc ttttgctgtt ttattttgta gaaagtcaag cgtcgtcatc
    16141 atgacatcaa tctgtttatc ttgcatcaca gggttcaatc ccttgctaaa atcaccaaca
    16201 gacaaatcag cgacatatgg aagaagaaac agaatccgtc gtgtttttgt aggagaagca
    16261 agaatctgtt ccggagaagc ggatttaacg aaactgcctt tcccgcgaac gcgataaata
    16321 agtcctaatt gctctaattc agtcaatgca cgtttggaag tgattctgct tactttatag
    16381 gtacgtgaaa gttctttttc agttggtagt tggctgtcta caggtaattc accagttaaa
    16441 atagccgttt gcaaatcatt aaatattgtc tggtacaatg gttgagtcat ataaacacct
    16501 cttaatgata tatcatattc aaataatacc ataatggatg aaaaagtaaa taaaaagata
    16561 ttttttgaaa gcgtagacat aattggctgt atgaagtata atctttaaag atatatcaaa
    16621 tagaaagaag gttttattag tggtttataa agatatacca aaatcggtgc agaaatttat
    16681 ggaagatatc acacaaaaat gtggggatat ccatgcggac tgggcaaaga attttacagc
    16741 agcatttgcc aatactctgt tgacaacagt caaaaagcaa aatgacggaa ccaccttttt
    16801 attgacggga gacattccag cgatgtggtt aagagactct actgcacaag ttcgtcctta
    16861 tttagtgatt gcaaaagaag atgaggaatt agctgacatg atcagcgggt tagtcaaaaa
    16921 gcaatttttt tatatcaata tcgatccata tgcgaatgcc ttcaacgaag aagctaacgg
    16981 tgctggccac caaacagatc atacaaaaat gaacgattgg atctgggaaa gaaaatatga
    17041 aattgattct ttatgctacc caatacagtt ggcctatctt ctttacaaaa atacaggaaa
    17101 aactgatcaa ttcaatcaat cttttattga aggtatcaaa aaaatcttgg acgtgttcaa
    17161 aacagagcaa gatcatgaac actctcctta tacatttgta cgagacacga caagaaaaga
    17221 agatacgctg atcaatgacg ggcgaggtgc aaaaacagct tttacaggaa tgacatggtc
    17281 tggtttccgt cctagcgatg atgcttgccg ttatggatat ctggtaccat ccaatatgtt
    17341 tgcggtggtc gtgttaggtt atgtggaaga aatttttcaa acgatactcg atgataaaga
    17401 aattgtacag caaacacaga aattgaagaa agagatccaa gaggggattc agaatcacgg
    17461 acgaacggtt aatcaaaaag ggcaaacgat ttatgcatat gaagtagatg gaatgggaaa
    17521 cgccacagtc atggatgaca gtaatgtgcc caatctgatc gccgcacctt acttgggata
    17581 ttgttctgaa caagaggaag attatctgca aacaagaaga acattgttga gtaaagaaaa
    17641 tccatatttc tatgaaggaa agtacgcaaa aggaattgga tcctcccata cgccagaaaa
    17701 atacgtttgg ccgattgcat tagctatgga aggaatgaca acgtcagaca aatctgaaaa
    17761 agagcggata ttgaatttac ttgtagcaac tgacgcagga acacacctta tgcacgaagg
    17821 attcgatgtg aacaatcctg aaaattatac gagagaatgg ttttcttggg caaatatgat
    17881 gttttgcgaa ttagtcatgg attactttga tattagagta gaaaagtagg aggtatcgta
    17941 aatgaaaaag aaagtatata tcatttctca tagccactgg gatcgtgaat ggtacatggc
    18001 ttacgaacag catcacatgc gactgattga attgatagac gatctactag agctatttga
    18061 agttgatccg tccttcaaca gttttcattt ggatgggcaa acgattattt tagatgacta
    18121 tcttcaagta aggccagaaa aaagacaggc aatccaacaa gcaatcaatg aaggaaaatt
    18181 gagaattgga ccattttata tcttgcaaga tgattttctt atcagctcgg aatcgaacgt
    18241 gagaaatatg ctgattggca tggaagaaag ccgtaaatgg ggaacccctg tcatgttagg
    18301 ctattttccc gatacatttg gaaatatggg acagacgcct caattaatga aacaagctgg
    18361 tatttcagca gcagcgtttg gccgtggtgt aaaaccaatt ggctttgata atcaagtttt
    18421 ggaagcagaa aactattctt cccaatactc agagatgtgg tggaaaggcc ctgatcaaac
    18481 agcaatcttt ggtctgctat ttgccaattg gtatagcaac gggaatgaga ttccagtaga
    18541 aaaagaagca gctcttgcct tttggaagca aaaattagcg gatgcagaac aatatgcctc
    18601 tacgaaccac ttattgatga tgaatggtgt tgatcatcaa ccggtacaaa aagatatatc
    18661 aaaagcaatc catctcgcaa atgaactgtt tccagattac gagtttattc attctaattt
    18721 tactgactat ctggaagcag ttcaaaaaga tgtgccagaa gacttaggct cagtagaagg
    18781 agaattgaca agtcaagaaa cggatggctg gtatacttta gccaatacag catctgctcg
    18841 ggtttactta aaacaatgga ataccaaggt ccagcgacag ctagaaaata ttacagaacc
    18901 acttgcgacg atggcttatg aagttagtgg aaactatccc catgatcaat tagattatgc
    18961 ttggaagaca ttaatgcaaa atcatcctca cgacagtatt tgcggttgtt cagtcgattc
    19021 tgtccatcgg gaaatgatac ctcgttttga aaaagcagat gaagttggaa agtatcttgc
    19081 acaagattcc ttagaacagc tgaccgcagc aatcgatacg accggattcc caaaagacag
    19141 ctttccattt gtcattgtaa atactgcagg aatggataaa actggtgaag cagaaataac
    19201 catcgaatta gaaagaaaaa gattcgctga aggaatacct gaacaactat atcaagagtt
    19261 agaaaatttg cctaaacgta agtatcatgt agaaacaaaa agtggggcca ctataccagc
    19321 tattttatct gaagaaactg tccaatttgg ttatgatttg ccgaaagacc gtttccgtgt
    19381 gccatatatg gcaagaatga tcaaagtgac tttaccatta gaaaacatgc cagcattttc
    19441 ttgggaaaca ttcgcgcttg tagaagggga agcgaaagca gaagaaaagg aaacaatgat
    19501 tcatcagagt ggacgaatca tcgaaaacgg ccctctacat ttgacgattg aaaaaaatgg
    19561 taccattaca atggaagatc gcaaaaacaa acgaaagttg aataatctgc atatttttga
    19621 agatatcggc gatataggaa atgagtatat ctttaaacaa cctttctgtg atcagccaat
    19681 cctatccagt aacaaagaaa actctgaagt aaaagtatta gtagatacac cggaaatagc
    19741 aaaaataagt attctacagg aaatggaaat ccctgtttct gcagacgagc gtttagaaaa
    19801 agaacagcag atggttgtgg aatttcgtta tcgaaaagca gaacgatcca aagaaaaacg
    19861 aatccttcaa ataaaaacaa tcatgacaat cagaaaagat agtaaaaaaa tcgactttga
    19921 aacgactctc gacaatcaga tgaaagatca tcgcttacgt gtcttgtttc ctacaaagtt
    19981 acatgtggaa catcacgaag cagatagtat ttttgaagtg gtgaagcgcc caaaccatgt
    20041 gtctaaaagt tgggaaaacc ccacgaatcc gcaacatcag caagcatttg tcaatattca
    20101 tgatgaagag tacggtgtaa cagtcggaaa ctttggtttg aatgaatatg aagtaacaga
    20161 agacggacaa atcgcagtga cgctacttcg cagtgtggga gaactaggcg attggggata
    20221 ttttccaaca ccagaagcac agtgtctagg tgagcatcgg ttcaattaca gcattgaact
    20281 acatggacca gaagaaaaat tctctactta tttgcatgca tacgcagcac agattccttt
    20341 ttccactcaa caaatcaaac atcatgaagg aacattgatt tcaaaacaac aatatttaac
    20401 gataaaaagt gaaacgtttg caattacagc tttgaaacgc agcaagttta gtgataaagt
    20461 agtagtaaga ggtttcaata tgtctagtca tttagaaaaa ttggaaataa ctaaagacaa
    20521 tggaaaaact gtcatattga atctgcttga agaaccaacg aagcaagcgg ttgtcccaat
    20581 aatccaacct tatgagattc ggacaatcgg attcgaggag gaaaacgaat gtatggcgga
    20641 atagaagcag gcggaacaaa atttgtgtgt gcggtttcag atggtggaga aattttagaa
    20701 aaattaagta tccctaccac aacaccagaa gaaacattgg atcaggtatt tgaatttttt
    20761 gatcgttatg aattgaaggg aatcgggatt ggctcattcg gcccgattgg tatcgacaaa
    20821 acaaaagata attacggata cgttttggca actcccaaaa aagggtgggc acaattcgat
    20881 tttcttggca caatcaagga acgctacaat attcctgctg cttggacaac ggatgtcaat
    20941 gctgctgcat atggggaatt gaagcaagga gcagcacaag gaaaaaatag ttgtatttac
    21001 cttaccgtgg gaacaggaat cggtgcaggt gtcgtgatca atgaagaaat tttttcaggg
    21061 atcgcacatc ctgaaatggg acatatttgg gtgaaacgcc atccagatga ccattatgaa
    21121 ggtacttgtc cttatcataa agattgttta gaaggattag cagcaggacc gtctattgag
    21181 gcacgcacag ggatcaaagg gcaagatctg ccgcaagatc atcctgtttg ggctattcag
    21241 gcttattata ttgctcaagc actgatcaac tatacgttga cgttagcacc tgaaaagatt
    21301 attttaggcg gtggggtaat gaatcaggat catctccttc aaaaaatccg tcagcaattt
    21361 gttgaattga tgggtggtta tatggaaaca cctcaagtaa gtgaatatat cgttcgttgg
    21421 ggactgccga atgaaagtgg gattatcggt agtttgctgt tagcagaaca cgaacatcaa
    21481 aagtaagcag acaaattaca caaaaagaac agagtcttat cattatctgc tgataagact
    21541 ctgttctttt ttatttctat tcccataatc caggtaaagc tttaccgact attttttctt
    21601 gagtaaatgg atggatcatg tgcagttcta atgcatggag catcagtcgt tcagctgttc
    21661 tattttggta gagagggtct ccaacgatcg gatgtccctt atgagctaaa tgaactctga
    21721 tctggtgtgt acgtccggtt tccaaaacac aatagacttg tgtttgttca gaaagtactt
    21781 tgttgacagt gacatgtgtc aaggcgctct gaccattttt atcatctacg accctttttc
    21841 tccgatcatg gcgatctcgt ccaattttat tggtgatggt catatctttg gcaatttctc
    21901 cccatacgat ggcttgatat ctccgataga tcattttttg ttccaacatt cttccaagta
    21961 tcggcaagac aaatggattt tttgcaaata aaacggcacc gcttgtttct ttgtccaagc
    22021 ggtggacaac gtaaggtcgt tgctgtttag tgtcgagata agcagctaaa tgattgagta
    22081 aggtactgtt ttctgtcggt tcatttggat gagtcttcat tcctgcaggc ttatccacta
    22141 taatcaaatg ctcatcttca tataaaacac ggatgtgctt cgcatctcct aattgaactt
    22201 cttgataatt ataatctgtt tcttcgaata acaatgtgat ctggtcattg ccctttactt
    22261 catgatggaa caaagcaggt tgttgattga tccaaacgtt tttacgtata cgcaaaaagt
    22321 gtcgaacctt tctaggaact aaccattcct tttcaagcaa gtcgcgaatt gttgtttcag
    22381 gatggttcat aggtaaagtg atcgtgattt tcacaagtgt actcctttca gtgcaatcgc
    22441 agactaattg taccattttt aaaacatatt taagaaaaaa cttacatttt tctgttcatt
    22501 cttatgttac tctacttgca ctaacgtaaa aaaacatatt gaactacaac ttaagcagga
    22561 agaaaacact ctttcactat ggtaaaataa gacgattgaa tcaaatagac aggagaagaa
    22621 gcaaatggat tttcaaagca ttttagaaaa gataaaagct gctttgatca gtttttggcg
    22681 ttggatcaga ccatatctcg gacaattcca tcgatggcga aagcgcatct ggaaaaaata
    22741 tcatgtgaac aaattgattt tgctgattgg tttgatctgt gtcttagtga caagtatcta
    22801 cctctttatt ttagcaaaac aagcaaatgt cgaaacgtta aaatcaggat taagccaatc
    22861 gacagtcatt tatgataaga aaaacgaaga agctggcaca ctgtacgccc aaaaaggaac
    22921 atatgtagaa ttaaacgcga tttctcccta tatccaagat gcagtaatct cgacagaaga
    22981 taggaatttt tacgagcatc acgggtttga tatcaaaggg attgcccgtg cagcagtccg
    23041 aatggtcttg tctggcggaa catcaggggg cggaggaagt acgatcactc agcagcttgc
    23101 caaaaatgcg tatctgacat tagaccaaac gtttgatcga aaagcgaagg aattattttt
    23161 ggcaatcgag atcgaaaaga agtacagcaa agaagaaatc ttgaccatgt atcttaacaa
    23221 tgcctatttt ggcaacgggg tatggggtgt ccaagacgct tccagaaagt attttggagt
    23281 agatgcaacg aatgttacgc tgtctgaggc tgctactttg gcaggaatgc tgaaaggtcc
    23341 tggtatttat aatccgatcg atcacccaaa aaatgcgaat gatcggaaaa acacagtact
    23401 aagtgtaatg gaagaaaacg gaaaaatcac aaaagaacaa gaacaagcag aaagccagac
    23461 agatatcagc gcgtatctta atgacacata cgataattca gattctggtt atcgttatcc
    23521 ttattatttt gatgcagtga tcgatgaagc agttaacgag tatggtcttg atgaagaaga
    23581 tgtcatgaat aaaggctata aaatctatac agctttagat caggattatc aaaagcaatt
    23641 agaagctact tatcaaaaca gtgcattatt cccagcaaat gcttcagatg gtgccatggt
    23701 ccaatctgct tcagtagctt tagatccgaa tacaggtggt gtacaagcac tagtaggacg
    23761 aagaggagat catgtcttta gaggattcag ctttgctaca cagatgcgtc gttcaccagg
    23821 atcaacgatc aaaccattaa gcgtatatac accagcactg gaagctggat acaagccttc
    23881 cagtatcttg aaagacgaac cgcaaagtta ttacgatgcg cataattttg acgggacata
    23941 ccaaggagaa gtaccgatgt atcaagctgt tgcacaaagt ttgaatttgc ctactgtatg
    24001 gcttttgcat gagatcggtt tacaaaaagg atatgataaa gcagaagaat tcggcctgcc
    24061 tttagcaaaa tctgataagt attatggctt agcccttggt ggactggaaa aaggagtatc
    24121 tccattgatc atggcgggtg cttacagtgc atttgccaat gatggtcaga tgtatacgcc
    24181 gcatcttatc acgaaaatcg ttgattcgac tggagcggtg atcgtcgata acacaaacaa
    24241 aaaaccaaaa caggtcatat caaaggaaac agccgatgaa atgaccagca tgcttcttgg
    24301 tacattttca aatggaacag cggctgctgc gaatcctgca ggttatacga tagcagggaa
    24361 aacaggaacc acagaaacta atttcgatgc cacaaaagca aatgaccaat ggatgatcgg
    24421 ttacacgcca gacgtagtga tttcgacatg gatgggttac gaagaatcca gcaagctgca
    24481 ctatttggaa gggacaagtg gaaatgttgt cgggaaagtc ttcaaatcag cggcagaagg
    24541 catccttccg tatacttcaa aaacaggatt tagtgtagca gatgcctatg ccacaggagg
    24601 acaagtggta gctgcagatc aagttggaaa cacaggaaat tctaatggag agtctggaaa
    24661 ttggcaagat aacttgaatc aatatggtga acaagcaaaa gaagggctga aaaacttcgg
    24721 cgatatggtg aaagaaggcg ttcagggaat tggtgaagcg gctaaagacc tctggcggaa
    24781 atatcaagga gagtagcccc tttgaaaacg aataacccca tgttacaata gagagtaact
    24841 actaaaagaa aagagggcat aacatgtcaa acatctacga tagcgcgaat caaatcgaac
    24901 gtgagatccg tgaattacca gaattcaaag cgttgaaaga agcgtacgag aaagtacaag
    24961 caaacgaaga agcacacaaa ctgttcaaag acttccaagc gatgcaaatc gaattacaag
    25021 aaaaacaaat gagtggacaa gagttcagcg acgaagatgc aatgaaagca caagaaatgg
    25081 ctgtgaaaat ccaatcagaa gaagtaatca atgacttgat gcaaaaagag caaggcttca
    25141 gtacaatcat caatgacttg aaccgtatca tcatgacacc agttcgcgac ctttacagcg
    25201 aataaaacta ttttatgaaa caacagaggg tgtgaaagag acgttcggcc ttgagcgatg
    25261 acaaaattgc ttcttattgc cgataggctg tctcttgaac accatttacc aagaggtcgt
    25321 gagcctaccg tttaactctg agttacaagg aacaatttca aaaaacagct tctcatgttt
    25381 gatgactatt gtgacttgtg accgaggagt tggcaggaga acaccgttta ttcagaaata
    25441 ggagctacga caaaagtctt gtcatagctc ctattttttg tcattcattt ttcaagcggt
    25501 agaaatagcc tttttgagct aacagttttt cgtaagtacc tatttcaacg atttttcctt
    25561 catcgatcac aacgatttta tctgcctctt tgatcgttga aagccgatga gccacaataa
    25621 aagtagtggg tgtttctgtc aggtaattca gggcttgctg gatttttttc tctgactggt
    25681 tatctaaagc tgaggttgct tcatctagca aaatgatttt tggctgacgt atcaacgctc
    25741 gtgcaatgga gatacgttgc ttttggccac cagacagctt gttgccatgt tcgcctacta
    25801 aggtatccag accatcagga agctggtcaa tcagtgaaga aagttgcgca gcttcgatga
    25861 cttcatctaa acgttctttt gatacgtttt tcaagccata agtgatgttc tctttgatcg
    25921 aagcggagaa caacaaagtg gtttgaggaa caacggctaa ataatttcgc acacttcgca
    25981 tatccagttc atccaaaggc tgatcatcta gaaaaatttt tcctgaagtc ggctggataa
    26041 atccaatcaa taagttaagg agtgtagatt ttccagagcc agatggccca acaaaagcaa
    26101 ttgtttctcc agctttcacc tctagagaaa aatgatcgat aatcggtttg agtgagtccg
    26161 gataaagaaa agaaacatcc tcaaaacgaa aatttccatc caaatgttgg atttttttct
    26221 taccgtgata agtttctaca tcgccagcaa tcaacagttc gccaatagaa gtgatagatt
    26281 ccatgccttt tgaaatcgta ggaatcagcg tgatcattcc agaaacggaa ttgacgatcg
    26341 tcgtgaaata cgtctgatac atcacgacat cgccgggttg gatgcgccct tgtaaagcta
    26401 atatcccagt gaaaatcaaa caaatcagct gaaatatctg aaaactagcc cagctgactg
    26461 atccgaaaac agattgcagg atatctaagt ggaatccttt ttcatagatt tgagtaaaat
    26521 gcttatttaa gcgagttgtt tcgtgttttt ctaacccatg agctcgagca atcggtgcta
    26581 attcgatcac ttcgatcact tttgcagaag tctcttccat ttcttgcctg aagtctgagt
    26641 tggactgtct gatcctttta cggaaaaaga gagtaacgaa tgcggcgaga ggaacagtca
    26701 ataagaaaaa catgaaaaca acaggacttt tataaagcgt gatacctaaa gctacgaata
    26761 aattgacagc gatatttaat ccgctgacaa atacttgaga ggacaacgtt tggatagctt
    26821 ctacatcacg gatgatcttt gactggattc gtccagattg caattctttt tgataaggaa
    26881 tagatagttc ttggattttc ttgataaggg tcgctcttag tccagcttct acttgacgga
    26941 tcgaagtgct atggtatttt acatgaaaat aattcgtcaa tatattttga ccaatcagac
    27001 aagccatcaa aaaagcgttg aaataaatgg cctgcaactg agaagaatct ttcgatgaag
    27061 ctgcattgat tacattggca atagcgatcg gcattaacca agatggaaga tgtttgataa
    27121 taaaaaagag tcctgacatg gcaaaacgcc aataatttcc ctgataaaga tgcagcaatg
    27181 tctgaaaagc atttgcatga tatttttgat aacttttctg tattcgttcc tccataatct
    27241 tctccttaac aatcttggta aataaactgc aagtgttcta ttgcttcagg aacataaatc
    27301 tcaggagtag cttcaagagt ggcgtacatt agaggacgct catatttgat ataatgcagt
    27361 accgggcgga aatctaaaca gcccttgcca acaggtacca tgcggatctt tccattttcc
    27421 atgatatagt ctttgagatg aagcgctgta atataaccat gtaaattgtc caaggcacct
    27481 cggatcactt tttcctgatc agcatgattt tctttatgca tcaaattcgc acaatctaaa
    27541 atgattttta aatttggtga agctacttca tcgattaaac gtttagctaa ctgatagtta
    27601 tagattggat gattgatccc tgcttcaaca gcaacagtta cgccaaaatt ttctgcatga
    27661 gcgactaatt caatgataga atttctggcg atctgataag cttcttcagt aaaattttct
    27721 tttgtgtaac cgacagagac actaccggtt tccgtagcaa tcatcttggc ttgataggca
    27781 gagcacagag caatttgctg tttgaaagtt gccaatgctt gtgcacgaac ttcttgattt
    27841 ttgctggata ggttgatata gcatcctaaa atggaaagtt cgatattgtt tttttggaag
    27901 tattcaccaa aataggcagc actcccaagt gtcatgtcag ttaatttaag ttgttcagga
    27961 aatgcttttt gtggtgcaaa ttggatatgg gacaagtgaa gctgtgatac tttttgaacg
    28021 atttcttcac gatttcgaac atctaaatcg tgggcacgga tgcctaaatt taatggaaac
    28081 atctcaaact cctttttgta aataattagc aagaggcaga cgggattctt ttatcaattc
    28141 aacaattatt cgggcaactt tatcagcgcc ttctggacta aagtgggtat tgtcttttag
    28201 tccttctggg taatttttac aagtatcagg tataagatgc aggtagaatt tttttgtctc
    28261 ttcttttgag aaggtctgga acaattcttg tgatcttgag aacacatcta aaagaggaat
    28321 atggtgtttc tcagcaaatg cacgcatcgc ttttggatag tctccaagta tatctggatt
    28381 caatgttccg ttttctaagt aatctcgtcg cgtcacagat gtcagtaaga ccatttggac
    28441 gttcgctttt tttgcgcatt caaaaaatat ctgcagattt tttaaatagc taccgaaggg
    28501 aagcgtacct ctttcttctt ggattttctg gtcgttatgt ccaaattgta caaataagaa
    28561 atcattcggc tgaaactgtg aagctagacg ggctaacctt ccttcctgga taaatgattt
    28621 ggtgctgcgt ccattctttg cttggttatt gatctggatc tcgggtgaaa aataagcaga
    28681 gattttttct ccccaacctg tctcgggacg cttgttttca tctttttctg ccgcagtaga
    28741 atcaccggca atatggatag taggcatgtt atctcttctt tcataaaggt ttataggcaa
    28801 tgaagcagaa cagcaagaga agaagacgga gcaaatttgg tccgtcttct tctctaaaac
    28861 gtggataaac ggattccgtt atttcactat cctgtttttg attcgttact gttactatta
    28921 atcaattagg caaaaaagcc aaagtaattt ttagcatttg tataactaat cgcttgaact
    28981 agacttccta aaagttgttc atcttcaggt gcttctcctc gcatgaccat ttcgcctaaa
    29041 aattcacaaa gaacacgacg gaaatattca tgtctagtgt aggacaggaa acttcttgaa
    29101 tctgtcagca tgccgacaaa attccccaaa gcactaccat cagcaaattg tgtcaattga
    29161 tcgcgcatac cagctcgagt atcgttgtac caccatccag aacctaactg cagttttcca
    29221 ttctgttctt tttggaaaca gcccatcaaa gctaaaatcg caggatagtc attcggattc
    29281 aatgaataaa ggatcgtctt tggcaactga tccgtttctt ctgcacggtt caataatttt
    29341 tgcagaggaa tagtcaaaga aagatcattc attccatcat agccggtatc gggccctaat
    29401 ttttccaaca tttgtgtatt acagttacga taagcatgga tgtgcagctg catcgtccaa
    29461 ttatgagaat gatagaagtg ggtcaaatgt atcagtgttt ctgtacgata agcatcgatt
    29521 tcatctgaag ataatagttt gttagctcgt gctttttgaa aaatctcttc taatactgtt
    29581 tcatccgctt ctttatagcg taagatgtct aaagcgtggt cggaaacacg acatccatgt
    29641 tgatggaagt attctatccg ctgccctaag acttctatca gttcttgata ggtggtgatc
    29701 gtttggtcag ctgcttcttc cagtagtgtg acccatgcat taaatccttc tttttcaata
    29761 ttgagtgctt tatctggacg gaatgaaggt agaaccttca aatctggttc ttcatgagca
    29821 agcaactgat gatagtgcaa gtcgtctgcg ggatcatctg ttgtacaaat cacttctaca
    29881 ttcgattttt ttgcgatttc ccgacggcga aaatcaggag ctgccaaaat ttcatttgct
    29941 tgtgtccaaa ttttctctgc attttcttca tttatcaaca agtcaatacc gaagaaacgc
    30001 ttcaattcaa gatgtgacca actgtaaagc ggatttccga taatttttgg tacggtttga
    30061 caccaagcta aaaatttttc aaaatcagaa gcatttcctg tgattttttc ttcaggcacg
    30121 ccgcaagctc gcatcaagcg ccatttgtaa tgatcgcctc ctaaccaagc ttctgtcaga
    30181 ttgctgaaat tgttgttttc gtaaatttct tttggatcta agtgacagtg ataatcaata
    30241 atgggcatgt tttttgcgta ggaatggtaa agctttttcg cccaatcgtc ttttaataaa
    30301 aaatcatcag ataaaaacat aaatcttcct tctttctttt tatactgttt tatgaaaatc
    30361 aaagtgttac tccgttttta aaaatggcaa tttctcgaac gtcattttgt tcatttttcg
    30421 tttcttctcc actagctaca gcgatgattt tttgaacaaa cgattctaag attacttcca
    30481 ttggttgttc taataagacg cctgcattaa aatccatcca atgacctttg cgttcaaaaa
    30541 gagttgtatt cgtagatacc ttgaccgttg ggacatatga gccaaacgga gtccctcttc
    30601 ctgtggtaaa caagaccaac tggcaatcgg atgaagcaag agcagaggca gctacaagat
    30661 cattcccggg agcttgcaat aaactaagcc ctggttttct gattttttcg ccatattgta
    30721 gcacatcaac gacaggagag cttcctgatt tttgcgtgca gcctaaagac ttatcttcta
    30781 atgtagtgat cccacctgct ttattaccag gagatgggtt ttcgtaaact ggttccccgt
    30841 aagacaagaa atactgtttg aagtcattga tcaagtcaac gattgagtca aaaacttctt
    30901 cattttccgc tcgtgccatc aatacttgtt ctgcgccaaa catttctggc acttctgtta
    30961 agatcgtgct gcctccttga gcaatcaagt agtcagaaaa tgcaccaagt aaaggattag
    31021 cagttattcc cgatagacca tcagaaccgc cgcatttcaa accgacattc aacttagaga
    31081 gcgggactga tatgcgctgg tcattttccg ccgctgttaa aagctcttct aatagagcca
    31141 caccttgttc gatctcatcg taaacatctt gagccactag aaatttcact cgttcttcat
    31201 catattcacc taaaattttt ttgaattcag gaactgtatt gttctcacaa cctaatccaa
    31261 atactaaaac tcctccggca ttgggatgat ttactgcgtc cgctaaaatt tctcgagtat
    31321 tttgatgatc ttttccaagc tgcgaacagc cataaggatg ctttaaaatc gtaatatggt
    31381 caaactgtcc cagatctgga tgtttttcct tgaactgttt gacgatcagt tcagcaatgc
    31441 cattcacaca cccaacggta ggtactaaaa acaaatcatt acgaatccct acttttccat
    31501 tttttcgcaa gtaaccttga aatgttcggt tctcaaaaag gtaatgactt ggatggacat
    31561 ctttttcata tcgataatcc aattcaccag aaagattggt ttttacatta tgagtgtgca
    31621 gccattgacc ggcagttacg tctgtcgtca catgaccgat tggatagcca tatttgatca
    31681 cgttgtctcc agctttcaaa tcacccaatg caatcttatg accttgcggg atatcttcaa
    31741 gagtggtgat cgtttgctga tcaatcgtca gttgtgtttg ttttggaata ggggttaatg
    31801 cgacgaccac actgtctcgt cgatttaatt tcattgttgg ttgcagggtt tcaagcatgg
    31861 accatcgttc ctttcatttc ttgtaatact gaccggacac ctttttgatc gatctttgca
    31921 gctatttctt gaactgtatc tgtcacattc ggtatttgag acaaatcttc tccccaaagc
    31981 gcagcgtttt gcaataatcg aggaatagct gttccattat cagaccaagc atcagtaaat
    32041 tcgcttatcg tcttttcatc atcatgtggc tttacttgat cgcctcgata cataagaagt
    32101 agcgcagcta gtgaaacagt gattaacggt ggtacctgat tttgttcttc gatatacttc
    32161 ttgaacactg gaagcagacg ggtgctgaac tttgaaacac tatttaatga aatcgcttct
    32221 aatttgtgat gggcaaaagg attcaagaaa cgttctttga tttggtcagc ataaatggct
    32281 aattcatctt taggtaaaga aagcatagga atcaattcta gattgaataa ctgatcaaca
    32341 tatgcggaga ataaaggatc tttcattact tcttctacag tttccaattc cgccaacaaa
    32401 gcaagaggga ccatcgctgt atgcggtcca ttaagcaaat gcaccttacg ttcacgataa
    32461 ggagtcatat catctgtgat gatgacattc aagccagcct gtttgaatgg tagttgattc
    32521 tccaattctt taggcccttc gatcacccaa agcatgaatg gttctgcttt gaccatcaat
    32581 tgatcttcat agttatgccg ttgattgaaa acacctgcat cttcgcgagg ataacctggg
    32641 acgatgcgat caactaaact acaacagaaa acattttctt tttctaacca ctcaacaaaa
    32701 gcaggttcta aatcccattt ttctgcgtag cgtaaaatga tttcttttag acgttcgccg
    32761 tttctgtcaa tcagttcgca aggaatgatg gtaaagccag ctttgtttaa tttgaaacgt
    32821 cggtataaaa aggctgtcag tttgcccggg aagctctgtg ggggagtatc tgttaaatta
    32881 tcagtctcca cgaattggat tcctgcctct gttgtattgg agacgatata ggccaagtcg
    32941 tcattttctg ctagagatag ataagcatcc cattcatcat aaggattgat catttgttct
    33001 actacggtga taatctcgga agtatcaacg atttcgccat tgctgagtcc ttgtaaaatg
    33061 accgtatata aattatcttg ctggttcatc atatttccaa gaccttgttc taaaggttga
    33121 atgattgcaa cattaccttt aaacaattgt tggttattca gctgctgaat ctgccaatca
    33181 ataaatccac gcataaaatt gccttcacca aactgaagaa cttttacagg tgcttcgttt
    33241 tttttcacta attcttttgt cagttttttc atataaactt cctttctgtg ttatgcacac
    33301 gttaacattt ttatataaaa ttacttgtag tttacaagta attgatcgac tcacgaagga
    33361 tcaacaaggt ggagagctca attttagatt gttcgatcgt cctgccttcg atcaaatcta
    33421 gtaaaagctt tgttccttct tgacacattt ctttcaaagg cttacgtacg gtggtaagcg
    33481 gcggattgag ataggagacg actggagaat catcgaaccc gatcagagaa atgttctggg
    33541 gaacacggag gccagctaaa tgtacagctc tcaatgcacc aatcgctatg tcgtcgttgc
    33601 cgcaaaaaac cagagtaggc aggtcagaaa gtgataatag gtattccatg ttttggaggc
    33661 cgctttctaa gctgtaatct cctgttaaaa aataggctgg attgacaggg atgctcgctt
    33721 tttttagact tttcaatacc cctttttccc gttcttttgt cgaacgaaaa tttttctttc
    33781 cgccaatata agcgatcttc gtatgtccgc atccaatacc gtaatctact gcctgcataa
    33841 caccggccgc atcatcggca gtgacgttga taaccgctgg atcattgatg tgacgattga
    33901 caacaacgaa aggaaattct ttttctttga gatagtcgat gaaaggctga tcatcatcac
    33961 tttgactcat aacgatcagt ccatcgtagc gttgcaggtt gatttctgtt aaatcaacaa
    34021 catcgatact ttcaacagat aaactgtaat ttgtgtctaa tactgaatgg atgcctgcaa
    34081 tggaatctgc taggaaattt gatgaagttc ctttatgcaa acttgagaaa aatagtccga
    34141 ttagatattt tttttgattc actaaacctt ttgcactata gtttggcgaa taattcattt
    34201 ccttagctag ttgtcgtatt ttttgttttg tttcattttt tattaaactg ctgttattca
    34261 aagcacgaga aactgtcgta tgagaaacgc cagctgcttt agcgatatct ttgatcgtta
    34321 ccattccatc attttcctta ctttttcact ttttgttaac gcattcatca tattacgata
    34381 actacgataa gtcaatagat ctttgatatg aaaagattgc acacgttaaa aattacttgg
    34441 atttttattt ttaattatgc tattcttgaa agcggggaat gctcatttaa ttagatgagt
    34501 atcaatttta cagaaaaatg aaagcctttt attttaaaag gaggagagga agaaaatgaa
    34561 gaaaagtaca gtattttata aaatattttt accgatgttt ttattaggag ccgtacttgt
    34621 cataggattt agtttattca tttaccagaa tacctatgaa tcgatcgaat cgagttattt
    34681 aacagacaaa aagaatttgc tcaggcaagt taaaactaac gttgaatgga aaattagaac
    34741 gattgaatat tcgttttcta cgtatggttc gaccaaaaat ttctcggaca tattcaagca
    34801 tccgctgaca tatacagatt actccactta ttctgaaatc aggaaagaac taaattttat
    34861 agaaacgatt gttatggatg ataataatta tgatttagtc agtttggctg gtaaatgggg
    34921 agtaatcaat ggatcgttga gtcaactgac cgaagaggaa gttgaaaagt atcaacagaa
    34981 gtacataaat aacaaaaata atttattttg gcaaaagaaa aacaaaggaa tcgaaatggt
    35041 gatttctttg ccaatgtttg aaaaagaaaa atatgcattg ggaattgcta cgatagagaa
    35101 acatacaata gaacaagttg ttggtgaaag tggagaagac atactttcta ttgaaaacga
    35161 agttggcaac ctttttacta ctcagacagt aaaaaataaa gagcaaccaa ctgtattaac
    35221 ggacgtggat ctagattaca atcagctgaa agtgataaga gaaaaagata aaacgtatat
    35281 agtactgaaa tcagattata ataattggat ttacagatta gaagttgact ctagtgcaat
    35341 tggcgcaatg atccgcaatc tgagaatcgg gctagttgca gtgtctttaa cactggtagg
    35401 actcattggt cttctttcct acttattttc ggacagattt gcccgtccga tttctcaaat
    35461 acaggaacgt ctgaatctta aatcaaaagg attcagtggg aaagaactag atttggtcgc
    35521 tcaatcagtc agtcggatca taggagaaaa agaagcgtta agtgcacact tagttaccca
    35581 aaagccacag ctggaaacgc tatttgtact tagtttattt agaaatcgag tagaaaggcg
    35641 agaactagat cagcggttgc agcaatttgg ttatagttct caaaatgact gctattatac
    35701 agctttggtt caaatcgatc tgttagaaga tagtcacggt ggggagcgtg atttgctact
    35761 tttagcgatc aacaacatga tcacggaaat tgtgccaaag gaaaatcgaa tgctgccgat
    35821 cgttttgaat gaagaaatgc aagccactat ttatcgaatg gataaagatg atgcagaagc
    35881 aagcaaaaaa gtcatggagt attgccgact cattcaaaaa cgtgtaaaag aatacttgaa
    35941 tttaactatc agtataggca tcagtaaccg gttcgaaacg ctgaatgaaa gcaagcaatc
    36001 ggttgatcgg gcaaaagaag ccctgtatta tcgtgtaaat actggtccaa gttcaattat
    36061 tttttatcag gaaatcgttc ctgctgctca tgaaaaaaca cttattagat atccgataga
    36121 agaacaaaat cgattgtttg aagccattcg ttctggaaat caggaagtga agaatttagt
    36181 acatgaattg attgatgcat tgtttgcaca aaataaaaat cctttgagtc gggaagtagt
    36241 ttcggtccgc ttgatcaatg aactcgttca attaggacag ttattgggag tagattcaaa
    36301 aaactttgat aacatgaaac aaatttatgt aaaagcactg aacaattatc atcccaaaga
    36361 actagaaaaa cttgttttag aacaattagt tctgccaatc acagaatgct ctcaagcaat
    36421 cactgatcat gaatttaaaa atttgtcgga gaatattatg catatcgtct acacagaata
    36481 tgatcgtgat ctgtctttag atgtgatcgc tgataggttg cattataacc ctaactattt
    36541 aagtaatgta tttaaaaagg aaacaggaga aaaatttggt gattttgttc aaaatcatcg
    36601 tttagaggta gcaaaaaaat ggttgaaaga gactaacctc tccgtaaaag agattgcaga
    36661 aagattgcaa tatcgaaatc cgcaaaattt tattcgtttc tttaaaaaga aggaatcgat
    36721 tacgccggga gaatatcgaa aaaaatattt gtgaatcaag aggctgtttt tcttgtggac
    36781 tgattcaagc aataaaagaa gttagaataa ccctagtatt tgtatttatg gatagttaaa
    36841 tttcaaggct aagcacgcaa aaagaactgt gacaaaagtc ttgtcacagt tcttttttac
    36901 gaataaatgg cgttcaagag acaggctatc gacaataagg cataattttg tgaaatatgg
    36961 gaagctattt ttcaaaatcg cttcttattg ctcctgtggg ttactcgtcg caatgtttgt
    37021 gagagttttt atcatcgctc aaggctgaac gtctctttca caacctctga tacacggtgt
    37081 tccaggagta gattcttcga aaaataagcc gaaatttcac aaaaatttaa gaagcaattt
    37141 tcgtaaatgc cttcttattt cttgaagctg accacttctg acacaactac cattttgcgt
    37201 tcagtacata atcacccttt gacagaacct acgagtgctc ctttgacaaa atatttctga
    37261 acaaaaggat aaacaatcag cataggcact gttgcaataa cgataacagc cattttaact
    37321 gtttgtgctg gtggaatcac atcaacggca ctactttctg cttgcatccc acttgataca
    37381 atgacgattt ggcgtaaaag aatctgtatc ggccatttag atgaatcgtt gatatacaaa
    37441 atagcagtca tataagtgtt ccagtaagta acagcataaa aaagcgagat agtagcaata
    37501 gacggtaacg ctaatggcag cataattttg aaaaatacac caaaatctgt gcaaccatcc
    37561 atttttgctg attcttccaa actatccggc aaggcttgga aaaagtttct cataatgatc
    37621 atattcattg cattaacagc cacaggcagg atcatggccc agtaagaatt gaccagccct
    37681 agagaacgaa caactaagaa cgttggaatc atccccccgc tgaacaacat agaaaataca
    37741 acaagaaagt tgacgaaacc tcgaccaaac aaatactttc tggaaagacc gtaggccatc
    37801 agtgaagttg cacacatact gacgatcgtt ccgacacctg taacaccaat cgaaacagca
    37861 agtgctctaa aaatggtcgg cgtagaaaga atgtagcgat acgcgtctaa agaaaaggta
    37921 gtaggaaaaa ggataaactt tttcgcgacg acttcttgtg tagaagcaaa agatgtagcg
    37981 atgatattta aaaaaggaac gatacagatc aaagctagca aaattagaaa cgtattgttg
    38041 aaaagtgtaa aacttctttc tcctatagag actttctttt tcatatagat aggctcctcc
    38101 tcaaaataaa ttaaatgttt ctgtttctat atgttcaatt tatcatcggt gaaagattac
    38161 gtatataatc atttcctatg ctaagtgtca gatagaaaaa aatagaaggt ttgctatgcg
    38221 tagtttattt gtgaaaaaat acactttatc gttttgtata gcacaaaaat cattgctatg
    38281 taagcgtttt tacttacggt aaaaatgatt atatacgtaa ttcagatagt tatctatcat
    38341 gaagtcataa gcagcaaaca ttcacaaaac taaggagggg cttatggaaa gtgtaaagaa
    38401 acaagaacca tttcaactga cgctatcgaa aaaagaagaa aagctcagaa gacgaaaaaa
    38461 aataattgca ggaatcaaaa cgaataaatt tttatatgtg atgattctgc cgggagtcct
    38521 ttatttcttg atttttaggt atcttcctat ggggggactc gttattgctt ttcaagatta
    38581 tcaaccattt ttaggaataa tgggaagtcc ttgggtcggt ttcaaacatt tcattcgttt
    38641 gtttacagaa ccgacattct ttacattatt aagaaatacg ttgatcttgt tcggattaaa
    38701 tatcttcgtg ttttttccga tgccaatcat tttgtcttta ctattaaatg aagtaacgaa
    38761 caaacgattg aaaaacggga ttcaaacgat catttacatc cctcacttca tgtcttgggt
    38821 aatcattgtt tcgattactt atgtattttt gaatgttgac ggaggggtat tgaatgaatt
    38881 ccttgcaaca atgggatttg aaaaaatcag ttttctaaca tcccctgaat ggacacgcac
    38941 agtatatatc ggacaagtga tctggaaaga gttgggctgg tctacgatca tctacctttc
    39001 agcgataact gcagtcgata cacagttgta tgaagctgca gaaatggatg gagcaggacg
    39061 cttacgcaaa acatggcatg ttacattgcc ggcaattcgt ccagtaatca ttaccttgct
    39121 gattctaaaa atcggtcaga cattagactt agggttcgag catatgtact tgttattgaa
    39181 ttcattgaat cgaagcgttg cagaaatttt cgacacctat atttatacag ctggattgaa
    39241 gaatggacag ttaagtttca gtacagcaat gggattgttt aaaggggtcg taggattgtt
    39301 tatggtaatt ttagctaata agattgccaa aaagctagga gaagacggcg tttactaaga
    39361 tagaaagaga gggaaagata tgctgcagtc attagataaa ataaacggaa ggctaaatcg
    39421 tattttacag ttggtttatt tgaattttct gtggggcggt ttagtcatcg caggtttggg
    39481 agtttttact ttcggtcctg catcgtatgc gatggttgca gtgattagaa aatggctgcg
    39541 ctcaaaagaa acgtttcctt tgacgaaaac gtacttcaca tattttaaag aaaactatcg
    39601 tgaaaccttg ctgatgagct ggatatatgg cgccatcggg attgttttac ttgtggatct
    39661 ttctttcata cacaattggt atcttaggat cgctttgtat attgctgcgt tcatatatgt
    39721 gctctcagca ctctatattt tcccaatcat ggctcattat gattggaaag gatatttttt
    39781 caaaatgaaa atggcatttt tattcggatt ctcacatctt cattactcat tagtgttgat
    39841 attaagtatg gtagggattt atctgatgat catccaaaca atgccaggtg ttttaacctt
    39901 tatcggtggc agtctcttct tttccgcagt cacgtggaca gcaaattcta tattttgcca
    39961 tttggaatgg aagaactcat atacagaaaa aacagaagaa aacaaaaata ttattaaaac
    40021 agtgaaggag aaaatttatg aaaaaagcaa cgctcgtaaa attaggtctt agttttgcag
    40081 gaattctagc acttgccgca tgcggcagca gttcaagtaa cagttcttca gagacagaag
    40141 atggaaaagc taaaatcact tggttaaata ttttgcatca tgcttcaccg cctacagaca
    40201 cgattgtgaa cttgatcgaa gaaaaaaccg atacggaact gacattcaac tggattcctg
    40261 atgcttcaaa agaagaaaga ttaacaactg cattggcttc tgatgaacta ggagatatcg
    40321 ttacacttca aattgcgatg ttgaaaaact cttctgtccg caatgcgtta aaatcaggcg
    40381 tattctggga tgtatcagaa tatttaaaag actataaaaa tttaagtcaa atttctgaag
    40441 atagaatcga agctgcgagt atcgacggtg ccttgtacgg cgtaccgata caaaaagact
    40501 acgctcgtgc tggcttagtc attcgaaaag actggttgga taatctagga ctagatgttc
    40561 caactaccat ggatgagctt tatgaagtag ctaaacaatt tacagaaaac gatcctgatg
    40621 gaaatggtgt ggacgacaca gttggttttg gcgatcgtgc gcctagtgat attcgttata
    40681 ccagctttaa acttttcact tcttattttg gagcaccaaa tggctgggct gtagacgata
    40741 atggcaaatt cactccagaa tttgatacag aagagtatat gaaagctttg aatttttcaa
    40801 gagatctatt tgagaatggc tatttggcac aagactttgc agttacccaa aagactgatc
    40861 agcaagaaca atttgcacaa ggaagaactg gaatctacac tggaatggtg gatatcaaga
    40921 atctgaagac gatgggtcaa ggtattcaag atgatatgga attagtgcct gtaaacaaga
    40981 tctcagatgg tgaaggcggc ggttatcatg tttggtcaga aggaaatggt attggcggct
    41041 tgctagcatt tcccaaatct caagtaaaag acgaagcaca attgaagaaa ttacttcaat
    41101 ttgttgatga tcttttagaa gaagatgtgt ataaattgat gactggcggt atcgaaggaa
    41161 cgcattatga attgaatgat gatggttcta tcgaatttat cgatatggat ctttggcaac
    41221 aagaggtgca accgttatca agctctcgtc ctagtgaagt gacgttctct ttcaaagata
    41281 cagaccctga aaaagaattc tctaatcaat tgatcaaaga aaatgaagaa tttgctgtaa
    41341 tggatccttc tgtgccattg gattcaccga cgaacaatga agtaggatca gaaattgaaa
    41401 aaatcgtcat tgatgctacg gtgcaataca tcatgggaca gcttgacgaa gatggattca
    41461 aaaaagctgt agaagactgg aaagcacaag gaggcgatcg aatcacacaa gagtacgagg
    41521 aagcatacaa agcagcggca cagtaatata gacgattggg acaaatactt cttggtattt
    41581 gtcccaaatc ctattttaat ttttaaagga gagcaaataa tggaaaactg gtcaaagaaa
    41641 ttagcaaaaa gtatcatgga aagaacacct cgcttatatg aagaaaagtg gtataaagga
    41701 aaatggtctt atgactatgg cgtcgtactt aaagggttcc aattactttg ggaacgaacc
    41761 caagaaaaaa tttattttga tttcataaaa gacaatatcg actatttcgt gcaagaagat
    41821 ggaacgatcc gtggctacag cgttgaagaa tacaatatcg accatgtaaa tacaggaaag
    41881 cttttctttt tattgtacaa agaaacaggc gaagaaaaat ataaaaaagc agcagaactc
    41941 ctttcccagc agttggcaaa ccatccacgc acatccgaag gagctttttg gcataaagaa
    42001 atttatcctt atcaaatttg gttggatggc ttatatatgg gttctccttt ttatgctgaa
    42061 tttatccaga attttagtga agaaaaagat taccaagatg ttttgagaca gtttgaaatc
    42121 agttataaac atctattcga tccaaaaaca aatttgctga ttcatgcatg ggatgaaaag
    42181 aaggtccaac catgggctga tccaaaaaca ggtctatccc atcatttttg gagccgatcg
    42241 attggctggt atttgatggc tgcagcagat acctatgaat tacttcatga agaaacagat
    42301 tgttctttgc tgaaagaaat tctagaaaag acactggctg cattacttcc atatcaagaa
    42361 gaaaacagcg ggacatggta tcaagtaaca acagaaacaa aacggaaagg aaattatctg
    42421 gaagcttccg gcagtagtat gtttgtttac gcaatggcta aaggaatacg tttgggactg
    42481 ctgaacaaag aagaatggct tccacaagtg aagaaatcac atcaaggatt acttgatgaa
    42541 tttgttctgg aaacaaaaga aggctggctg aacttgaata aaaactgtca agtagcaggg
    42601 ttaggcggag cagatcagcg agatggttca tatgcttact acatcagtga accaattatt
    42661 tgtaatgatc aaaaaggcgt tggtgcattc ttgcaagcat tgattgaagt ggaggcatta
    42721 taaatatgta tgatattttg acatttggcg catcaattga tgaattgaat actgaagcaa
    42781 tacaacaagc cattgatgct gcagcaagtg acggaggcgg gaccgtagtt gttccagcag
    42841 gtgaatttct aacaggagct ttatttttaa aaagcaatgt cgaacttcat ttaagcgcag
    42901 gagcggttct gaaattctct gacgatccaa aagattatcc tgttgttcat tcacgttggg
    42961 aaggagtcca tcgaaaagtt tatgcttcct gtatctatgc tcaaaatgta gagaacatct
    43021 ctgtcactgg tttcggtaca ttagatggaa atggaaaaaa atggtggcat acttttagaa
    43081 atgaaccaga taatctcgct tatcctcgtc ctaaattgat gagtttccat aactgtcatc
    43141 gaattactgt gaaagatatt aaattgatcc agtcaccaag ttggacgatc aacccgattc
    43201 tttgcagtaa tgcaacgttt gataacttga cgatcttgaa cccagcggat tcaccaaata
    43261 cagacggaat tgatccagaa tcatgcaaaa atgtgcgtat tagtaattgc catattgacg
    43321 tgggagatga ctgtattgca atcaaggcag ggactgaaga tacgtacgaa aggatcgctt
    43381 gcgaaaatat cacgatcaca aattgcacga tggtccatgg acatggcggt gtcgtattag
    43441 gaagtgaaat gagtggtagt attcgtaata ttaccatttc aaattgtatc ttccaagaaa
    43501 ctgatcgagg aatacgattg aaatctagac gaggtcgagg cgggatcgtt gaagatattc
    43561 gagtatccaa tatagtcatg gataatgtga tgtgtccatt tatactgaat ctttattatt
    43621 tctgtggtcc gcgagggaaa gaaccgtatg tatgggaaaa aaaggcttat ccgatagatg
    43681 aacggacacc agcattcaga aggatccatt tttcgaatat cacggcaagg aatgtccatg
    43741 cttctgcagg ttttatttat ggattagcgg aacaattcat acaagaaatc acatttgaca
    43801 atatcgatgt ctcgatggcc caagatgcag ttcccggtgt tcctgctatg atgacaggaa
    43861 tagaggaaat gagcagacaa ggattttata ttggctttgc gggtaaggtt tcctttaacc
    43921 agatgtcgat tgaaaatcac gagggaccag catttctatt agaaaatact aaagaaatca
    43981 gtattgagaa attccaatca aatcaatcag aaaatatgga tgaactggtt gttcgtaaaa
    44041 actttgctta tgcagttgaa tgatttttta ggtgctccat ctagcagaca tacccctagt
    44101 ggaaaacgat tataccgaga aaaacaaaac ggttttatgc ttgaatacta tcaattgtac
    44161 ttaaatgaat tggaaacagt accagccctc tatgcttatc cagaaaaaga aggaccgttt
    44221 ccgccggtcc tttacctgca ttctcacggc ggagattttt cagtcgggaa aagtgaattg
    44281 atccatggtg ctccttactt agcttctcca tcttttgcag aagtactgac aggaatggga
    44341 tatgctgttt ggtcgattga tgcttgggga tttgaagaac gcggaggtat ttctgaaagc
    44401 gagttgttca aacaattttt attaacagga aaaacgttat ggggaatgcg tatttatgat
    44461 gtgatcagct tgatcgatta tttggagaca agggaagaca cagatactca acggctcgca
    44521 acgattggtc tttctatggg aggcttatta agctggtggg tgacagcatt ggattctcgt
    44581 gtcaaagttt gtattgatct agcggcacag gtggacatcg aaatactttt gctacatcgc
    44641 cgcctagatc atcatgggtt ttattactat gtgccaaatc tattgacagc ttatacaacg
    44701 ttagcaatcc aagaaaaaat cgctccgcgc cctcgtctta gcttagttgg aaaaaacgat
    44761 acgatgtgct tggacgaagg cgtactgaag ctgagaaaag ggctggacaa aaagtatcaa
    44821 tcaatgggta gtccagaaaa cttttcttct ttttctttaa caggcggtca tatggaaaca
    44881 caagaaatga gaaatatttg gaaacaattc ataagggagc atttatgagg atgaaagaca
    44941 aactgataat tggaaagaat gctatagata ccccgacgga ttttgaaact atccaagaag
    45001 gaatcgatta tttggcaggc ctgccggata gccatccaaa ggtcttgata gtaaaagaag
    45061 gaagatatga tgaatatata gaaagcagat tatccaattt taaacttgtg ggagcaggaa
    45121 cagtagttat ctccggtaat cgttttgcaa aacaggagta tcttgacggt agccagttag
    45181 ggactttccg aagtgctact tttttcttag aaggatcgaa tgtcgtatta gaaaatcttc
    45241 acatcgaaaa tacagcaggt ccaggagaaa aggtaggaca ggctgttgct ttattcaatt
    45301 caggagatcg cgtatcttta aaaaactgtc gactatctgg ttatcaggat accttatgca
    45361 cgggtccgtt gcctgatttg caaaaagatg gtactccttt ttgtacaccg gcttctgcca
    45421 atcctgagta ttgtcgtcag acctatgaat attgctggat tgaaggaacg gtggatttca
    45481 tatttggtgg ggcagatgcc gtttttcatc agtgtcatat ccatagctta gccagtaaaa
    45541 atcctggata catcacagca gcatctacgc cgaaaaatca aaaaacaggg tatcgttttg
    45601 aaagctgtac cttgactgca gaacctgata ctagaaacgt ctatttagga agaccgtggc
    45661 gtccgtatgc aaatgtctta tttgaagcgt gtaagatagg tgggcatgtt cacccaactg
    45721 gttgggataa ttgggatgac aagaaaaatg aacagactgt caaattctct gaacgtaata
    45781 acaattatga aacatcgatc aaaagacctg aatggataca tttagaaaag cagggggttc
    45841 agaaatgaaa agaaaatggg aactattgct aggaatggtc ggtggcagtc tatctttgat
    45901 tttctttggc gggttagcag ttactttaag taatatgtct gcgtcagagt ttaaaaaaag
    45961 ttatcagtcg cttgctgtag atcattcaac tttgtcttta gaaaatacat ttggattatt
    46021 acaggacatg actggtctgt ttgccgttgt gttgtttatt tcacttgctt ttttagcagt
    46081 agcattgttt ttgactgcta aaggaaaata cttaacaact gccactggtt tgtattttat
    46141 aacaggattc atattgttga ttggcactca gtttattgcg tttccttttg cattttttta
    46201 tttcgcagct ggggcattca gtttatatcg agtaagaatg agaaaaggag cctgaatcat
    46261 gcagactatc aaagtgaaaa atcctatttt aagaggattc aatccagatc ctaatatatt
    46321 aagagtgaaa gagcggtatt acattattgt ctcctctttt gaatggcttc caggaatccg
    46381 agtatatgag tcgaaaaatt tagtcaactg gtcacataca acggatattt tgaccactca
    46441 agtatctttg caaggaaatc ctacaaatgg aagtatatgg gcgccacaaa tcagttatgc
    46501 tgatggattg ttttatcttg tttacacaga tgtcaaaagt gcaggccgtc cgtttaaaga
    46561 tgtccacaat tatttgatca ctgcatctga gatcacaggt ccttggtccg aaccaattta
    46621 tttaaatagt aacggctttg atccttcttt gttccatgat cttgaaacgg gaaaaaaatg
    46681 gcttgtcaat gaactatggg attatcgtct ggacacaccg aataaatcca gcggtatcgt
    46741 attacaagaa tatgatgcag agaagaagaa gcttgatggt cccatctata aaatatttgc
    46801 aggtacagaa ttagcgaaaa cggaggcacc acatttgtat tttcgtaacg ggtattatta
    46861 tttgatgacg gcagaaggcg gtacaggaag aggacattca gttacagttt gccgagcaaa
    46921 gaatattact ggtccttacg agcttgatcc tggttttcct atgctgacag ctagtgacaa
    46981 acctgcttct acattgcaat gtacaggaca tgggagctta gtacaaaccc cgtcaggaag
    47041 atggtacatg acatatcttt gtaccagacc attaaacggt gcggctattt tgggaagaga
    47101 aaccgctatt caagaagtgt attggacgtc cgacgattgg ttaagactta aagatagcca
    47161 gacaactccg atggaagaaa taatgattga gacaaatgaa gaggtacaac aagaaaaaga
    47221 tcatcagttt atggatacat ttgaaggaga gctaaagaag aactggaatg ccagacgcat
    47281 tttgccaaat gctgattggt gtgatacaaa atgtagacca ggttttttac gtctgatttc
    47341 cggtgaatct cttcaatcta ccttcgaaca tcatttgtta gctattcggc aaacggattt
    47401 tacttttgat gcggagacag catttgaata catgcctaat aattttaatc agatggctgg
    47461 actggtactg tacttaaatg aatccaatta tatttattgt tatgttactt ggaatgagga
    47521 caagggaaaa tgcctacgta tgatagaatc agaaaacgga gtagcacaaa tagaagattg
    47581 gacgattcca ctcacagaaa agaagacttt tttgaaagtc actgtaaaga aagagacagc
    47641 acagtttcat ttgaaagacg tatcaactga agaatggaag aaagtaggtc cgcaaaaaaa
    47701 catgtgtata ctctcaggag ggtttactgg aaattttatc gggatagcag tgcatgatct
    47761 aaataaaaaa agaggaagtt atgctgattt tgagtttttc aagtatgatg gaaaagatca
    47821 tctgtaattt aattctttaa taaatgaggt tcactaactt attttcacta gttagtgaat
    47881 tttt
//
LOCUS       DADVXU010000011        44860 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.011, whole genome shotgun sequence.
ACCESSION   DADVXU010000011 DADVXU010000000
VERSION     DADVXU010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 44860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.011"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            212..604
                     /locus_tag="KB953_000619"
     CDS             212..604
                     /locus_tag="KB953_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBB6865617.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="KB953_000620"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="KB953_000620"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HBB6865618.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="KB953_000621"
     CDS             3165..4775
                     /locus_tag="KB953_000621"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HBB6865619.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="KB953_000622"
     CDS             5067..5936
                     /locus_tag="KB953_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HBB6865620.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="KB953_000623"
     CDS             6101..7372
                     /locus_tag="KB953_000623"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBB6865621.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="KB953_000624"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="KB953_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBB6865622.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="KB953_000625"
     CDS             8763..9026
                     /locus_tag="KB953_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HBB6865623.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="KB953_000626"
     CDS             9471..9920
                     /locus_tag="KB953_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBB6865624.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="KB953_000627"
     CDS             9977..11395
                     /locus_tag="KB953_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBB6865625.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="KB953_000628"
     CDS             11395..12255
                     /locus_tag="KB953_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBB6865626.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="KB953_000629"
     CDS             12429..13202
                     /locus_tag="KB953_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBB6865627.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="KB953_000630"
     CDS             13349..13714
                     /locus_tag="KB953_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HBB6865628.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="KB953_000631"
     CDS             complement(13862..15070)
                     /locus_tag="KB953_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB6865629.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="KB953_000632"
     CDS             complement(15089..15367)
                     /locus_tag="KB953_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865630.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15521..16216
                     /locus_tag="KB953_000633"
     CDS             15521..16216
                     /locus_tag="KB953_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBB6865631.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(16658..17455)
                     /locus_tag="KB953_000634"
     CDS             complement(16658..17455)
                     /locus_tag="KB953_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HBB6865632.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="KB953_000635"
     CDS             complement(17452..18636)
                     /locus_tag="KB953_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HBB6865633.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="KB953_000636"
     CDS             complement(18661..19908)
                     /locus_tag="KB953_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBB6865634.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="KB953_000637"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="KB953_000637"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBB6865635.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="KB953_000638"
     CDS             21888..22202
                     /locus_tag="KB953_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBB6865636.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="KB953_000639"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="KB953_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBB6865637.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="KB953_000640"
     CDS             22887..23531
                     /locus_tag="KB953_000640"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HBB6865638.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="KB953_000641"
     CDS             23546..23875
                     /locus_tag="KB953_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HBB6865639.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="KB953_000642"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="KB953_000642"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBB6865640.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="KB953_000643"
     CDS             24863..25687
                     /locus_tag="KB953_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HBB6865641.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="KB953_000644"
     CDS             25680..26027
                     /locus_tag="KB953_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HBB6865642.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="KB953_000645"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="KB953_000645"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBB6865643.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="KB953_000646"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="KB953_000646"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBB6865644.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="KB953_000647"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="KB953_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HBB6865645.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="KB953_000648"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="KB953_000648"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HBB6865646.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31657..32451
                     /locus_tag="KB953_000649"
     CDS             31657..32451
                     /locus_tag="KB953_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6865647.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="KB953_000650"
     CDS             32441..34483
                     /locus_tag="KB953_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6865648.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="KB953_000651"
     CDS             34764..34913
                     /locus_tag="KB953_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HBB6865649.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="KB953_000652"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="KB953_000652"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HBB6865650.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="KB953_000653"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="KB953_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HBB6865651.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="KB953_000654"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="KB953_000654"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HBB6865652.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="KB953_000655"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="KB953_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HBB6865653.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="KB953_000656"
     CDS             39330..40412
                     /locus_tag="KB953_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HBB6865654.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="KB953_000657"
     CDS             40412..41215
                     /locus_tag="KB953_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBB6865655.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="KB953_000658"
     CDS             41285..42025
                     /locus_tag="KB953_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBB6865656.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="KB953_000659"
     CDS             42036..43040
                     /locus_tag="KB953_000659"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBB6865657.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43085..44782)
                     /locus_tag="KB953_000660"
     CDS             complement(43085..44782)
                     /locus_tag="KB953_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865658.1"
                     /translation="MKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLE
                     KPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTL
                     TVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMY
                     KQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYY
                     WSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLAC
                     IGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISY
                     NLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLM
                     HTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLP
                     EELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYD
                     TNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT        14421 a         7827 c         9341 g        13271 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtag ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcgaaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg tgcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    43141 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    43201 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    43261 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    43321 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    43381 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    43441 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    43501 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    43561 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    43621 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    43681 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    43741 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    43801 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    43861 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    43921 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    43981 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    44041 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    44101 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    44161 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    44221 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    44281 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    44341 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    44401 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    44461 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    44521 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    44581 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    44641 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    44701 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    44761 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    44821 aataagcaat tcctgttaaa aataacaatg ataatataat
//
LOCUS       DADVXU010000012        43123 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.012, whole genome shotgun sequence.
ACCESSION   DADVXU010000012 DADVXU010000000
VERSION     DADVXU010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 43123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.012"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(12..617)
                     /gene="dhaL"
                     /locus_tag="KB953_000661"
     CDS             complement(12..617)
                     /gene="dhaL"
                     /locus_tag="KB953_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HBB6865659.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(645..1655)
                     /gene="dhaK"
                     /locus_tag="KB953_000662"
     CDS             complement(645..1655)
                     /gene="dhaK"
                     /locus_tag="KB953_000662"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HBB6865660.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(1630..2076)
                     /gene="dhaM"
                     /locus_tag="KB953_000663"
     CDS             complement(1630..2076)
                     /gene="dhaM"
                     /locus_tag="KB953_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HBB6865661.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            2179..2499
                     /locus_tag="KB953_000664"
     CDS             2179..2499
                     /locus_tag="KB953_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBB6865662.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(2611..3480)
                     /locus_tag="KB953_000665"
     CDS             complement(2611..3480)
                     /locus_tag="KB953_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBB6865663.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(3576..4880)
                     /locus_tag="KB953_000666"
     CDS             complement(3576..4880)
                     /locus_tag="KB953_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBB6865664.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(4877..5386)
                     /locus_tag="KB953_000667"
     CDS             complement(4877..5386)
                     /locus_tag="KB953_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865665.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(5561..7696)
                     /locus_tag="KB953_000668"
     CDS             complement(5561..7696)
                     /locus_tag="KB953_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBB6865666.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(7779..9434)
                     /locus_tag="KB953_000669"
     CDS             complement(7779..9434)
                     /locus_tag="KB953_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBB6865667.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(9436..9750)
                     /locus_tag="KB953_000670"
     CDS             complement(9436..9750)
                     /locus_tag="KB953_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBB6865668.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            9920..10180
                     /locus_tag="KB953_000671"
     CDS             9920..10180
                     /locus_tag="KB953_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HBB6865669.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            10194..10508
                     /locus_tag="KB953_000672"
     CDS             10194..10508
                     /locus_tag="KB953_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HBB6865670.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(11238..11792)
                     /locus_tag="KB953_000673"
     CDS             complement(11238..11792)
                     /locus_tag="KB953_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBB6865671.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(11918..13847)
                     /locus_tag="KB953_000674"
                     /pseudo
     CDS             complement(11918..13847)
                     /locus_tag="KB953_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(14153..14869)
                     /locus_tag="KB953_000675"
     CDS             complement(14153..14869)
                     /locus_tag="KB953_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HBB6865672.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(14870..16693)
                     /gene="glpO"
                     /locus_tag="KB953_000676"
     CDS             complement(14870..16693)
                     /gene="glpO"
                     /locus_tag="KB953_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HBB6865673.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(16707..18203)
                     /gene="glpK"
                     /locus_tag="KB953_000677"
     CDS             complement(16707..18203)
                     /gene="glpK"
                     /locus_tag="KB953_000677"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBB6865674.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(18339..19799)
                     /locus_tag="KB953_000678"
     CDS             complement(18339..19799)
                     /locus_tag="KB953_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6865675.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(19789..21084)
                     /locus_tag="KB953_000679"
     CDS             complement(19789..21084)
                     /locus_tag="KB953_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBB6865676.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(21416..22093)
                     /locus_tag="KB953_000680"
     CDS             complement(21416..22093)
                     /locus_tag="KB953_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HBB6865677.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(22123..23214)
                     /locus_tag="KB953_000681"
     CDS             complement(22123..23214)
                     /locus_tag="KB953_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HBB6865678.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(23315..23749)
                     /locus_tag="KB953_000682"
     CDS             complement(23315..23749)
                     /locus_tag="KB953_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBB6865679.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(24333..25790)
                     /gene="ascB"
                     /locus_tag="KB953_000683"
     CDS             complement(24333..25790)
                     /gene="ascB"
                     /locus_tag="KB953_000683"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBB6865680.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EWVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(25787..27682)
                     /locus_tag="KB953_000684"
     CDS             complement(25787..27682)
                     /locus_tag="KB953_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBB6865681.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(27740..29515)
                     /locus_tag="KB953_000685"
     CDS             complement(27740..29515)
                     /locus_tag="KB953_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBB6865682.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(29565..31061)
                     /locus_tag="KB953_000686"
     CDS             complement(29565..31061)
                     /locus_tag="KB953_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBB6865683.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(31255..32877)
                     /locus_tag="KB953_000687"
     CDS             complement(31255..32877)
                     /locus_tag="KB953_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBB6865684.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(33205..34518)
                     /locus_tag="KB953_000688"
     CDS             complement(33205..34518)
                     /locus_tag="KB953_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBB6865685.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(34763..34987)
                     /locus_tag="KB953_000689"
     CDS             complement(34763..34987)
                     /locus_tag="KB953_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865686.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(35007..35429)
                     /locus_tag="KB953_000690"
     CDS             complement(35007..35429)
                     /locus_tag="KB953_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HBB6865687.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(35571..36281)
                     /locus_tag="KB953_000691"
     CDS             complement(35571..36281)
                     /locus_tag="KB953_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865688.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(36299..36796)
                     /locus_tag="KB953_000692"
     CDS             complement(36299..36796)
                     /locus_tag="KB953_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB6865689.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(36833..37285)
                     /locus_tag="KB953_000693"
     CDS             complement(36833..37285)
                     /locus_tag="KB953_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBB6865690.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(37420..38025)
                     /locus_tag="KB953_000694"
     CDS             complement(37420..38025)
                     /locus_tag="KB953_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBB6865691.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(38475..39095)
                     /locus_tag="KB953_000695"
     CDS             complement(38475..39095)
                     /locus_tag="KB953_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HBB6865692.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(39097..39966)
                     /locus_tag="KB953_000696"
     CDS             complement(39097..39966)
                     /locus_tag="KB953_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HBB6865693.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(39989..40396)
                     /locus_tag="KB953_000697"
     CDS             complement(39989..40396)
                     /locus_tag="KB953_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBB6865694.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(40415..41704)
                     /locus_tag="KB953_000698"
     CDS             complement(40415..41704)
                     /locus_tag="KB953_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBB6865695.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            41762..43101
                     /locus_tag="KB953_000699"
     CDS             join(41762..42203,42203..43101)
                     /locus_tag="KB953_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HBB6865696.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT        12346 a         9168 c         7270 g        14339 t
ORIGIN      
        1 ttttattgaa cttaaggttg attgatcaca tctaaaagcg ttgaaaataa aatggcacta
       61 gaagcagctc ctgggtcgat atgtccgatt gcacgctctc ctagatagga agctcgtcct
      121 tttttagcta ccaagacttt ggtgtgttct tttgcctggt caatgatttc ttttgttaaa
      181 ttccctgcct tcaatgcctc tgtgactggc agccaaacat ctaacatcgt tttatcgtca
      241 gcttctgcct ttcctcggct ttgaataccc gctgttccct gctcgatcac ttcgcctaat
      301 ttttcttgag aatcaataga atcgacctct tttgtcgctt tcatcatgtt catgaatgca
      361 gatccgtata acggaccaga agccccaccg actttcgata tcatcgccat agagaatact
      421 ttgaatgtat cgctgattgt ttcaggatga tcggcttgaa aagcttcttc ataagctgaa
      481 acccctcttc ccatgttatt tccatggtct ccatcgccaa tcggggtatc tagatcactg
      541 agatattgtt tgttctgatt gattgcctct gcaaattgcg tgagccattc ttggatttct
      601 tgaacggtta gtttcatatt atcacccttt tatcttgaga ttttttacca tgaaatcgtc
      661 tgtacattgc tttctaaaga atctttccat tgtgtctccg tcaagtcgat cagtgttaga
      721 gaaagacctt gcatatctag tgaggtcata aaattaccga cttttcggaa aactacattg
      781 acgcctcttt cttccaacag agctaaaaca tcattcatga acacgaactg ttccatcaaa
      841 ggcgtcccgc ccattccgtt gactagtaca cctgcttctt ttggtaaatt cgagtaatca
      901 tctaaaatct tagtaacaag ttcctttgcc agtactttag aaggctgcat cttttctcga
      961 cgatagcctg gctcaccgtg gataccaaca ccatactcga tctcatcttc tcctagttcg
     1021 aatcctggtt tccctacttc tggtacagtt gctgctcgaa gcgctagacc gatcgtctta
     1081 gttgcctgta cgaccttttc tcccagttct ttcaactcag ctagggaagc accgtttctt
     1141 gctgcatcgc ccacgatttt gtgtacaagg acagtacctg caactccgcg ttttccagca
     1201 gtatacgtac tattttcaac ggcgatatca tcatctacga tcacactttc tacttcaata
     1261 tcgtccatag cagctaattc ctttgccata tcgaagttca atgcatcccc tgtataattc
     1321 ttaacgatca gcaaaactcc ttttccacta tcggcttcct taatagccgt ctgaatctga
     1381 tctggagttg gagaagtaaa cacatcacca agcaccgctg cacttaacat cccttcccct
     1441 acaaacccgg catgggatgg ttcatgtccg cttcctccac cagaaaccaa accaacttgt
     1501 tccgctttct gattcttggc tacaacacgg gaagattcta cacgatgaac aagttctgga
     1561 tagcttttta ctaatccttc cagcatttct tcgacaatat tgcttggatc attcatgatt
     1621 tttttcaatt tattcccatc ctttctttcc ggcattttgt gcttctgtta agatttgctg
     1681 agtatcatct gttataccgg cagtgatggc tgctgcaaaa gctccttcca ctaaaggcgc
     1741 atccaccaga tgataacgtt gctgctgctc ttcttccaac atgtcttttg ccagttccga
     1801 gttcaatacc gcgctaccga tatccgcaaa aacaaaataa ctagctgcat cgcttgattc
     1861 attgactgct tctacgattt tcatcgggtc tgatccgatc tcgccattta ttccgccgag
     1921 agaaaaaatt tgcacctctt cggatttttg catctgctca atcatttctt taataccatc
     1981 tgttactttt tgactatgag aaataagcaa aatacttttt ttcacaagaa tcttcctttc
     2041 ttttacatag ttgctaacga ttggtttcgc tttcattaat gaacaattca agtatataat
     2101 aaatttctaa ttttgggaaa atatgtgtag tgcttcttga agtttgttat actttaatta
     2161 aaagagagga gtgatcgaat gattacaaca acaacgaaca gtgtagaggg aaaagccgta
     2221 gtagcttata aagggatcgt ttttggtgag gtcattactg ggatccatgc atttaaagac
     2281 ttggaggcgg ggttgcgaaa tatctttggc ggccgctcaa aaagctatga aaacgaacta
     2341 atgggtgcaa gagaagaagc acttgccgaa atggccgaac gtgcaaaagc attaggagct
     2401 gatgcgatca ttggcgtaaa aatggactat gaagtattag gcagcgacaa tggcatgctg
     2461 atggtcacat gtagtggaac agctgtctcc acgagataag taggggacat ctgttttaat
     2521 aactaaagag agatgggaca tgtagtggca ctcaagaatt agatttttca gtctaacttc
     2581 gaggtactct accttcccat cctctctttt ttatttcttt ttgcggatga ttccgtaagc
     2641 tgctgcgact aacacacccg cccctgctaa ggaataactg acagaagctg ttttttcatt
     2701 tgtttttggc aaagtctgac ttgttgattt ggatggctct gtcgtttgct gaactgtttc
     2761 tgtcgtgctt ccagaggtca ctgatttttc tcctgccgaa tgcaaaatat cttttattcg
     2821 ttggaaacta gctgctaagt cagctttttt tgcttcaaaa tcattagcaa ctgtttgtgc
     2881 ttcgctgatc tttccgtgga ttgcttgact ggccgctgtg atatccgctt gagtttgact
     2941 tagctttcca gaagctgttt gagcaacctg atttaattgc tgattgaatt cttttgcttg
     3001 ttggatcttc ccgtcaagat ctgtttggac agcttccgct tttgctttcg cttcggcaag
     3061 ctttttctca agttgtgcac ggtcatttcc cactttattt ttaagatcag ctaaatcttg
     3121 atcgattgct gcttggtctt ctttcaattt gttttttgca gcttctaatg cttcttcgag
     3181 ttcttttctt ttattttcca agtcttgcaa atgatcgatt tggtcagaag aggtagaact
     3241 atttattgat gtatcttctg aagatgaaat cgtttctcct gaattatctg tcggtacatt
     3301 ggaagtcatt tctgtttccg aggcaccgtt ttcagcacca gaagagctta tggttgttga
     3361 aggttctgtt tgagcaggtt cagaagtttc cgcaaaagct gtctgtccca ataagaaaat
     3421 actggaaaca gcaattggcc agattaacgg cttcattctt tttttcatca cttttcccat
     3481 ccttactact ttttaactaa tttcttatta taagcaaaaa tgtttggaag aaccataaac
     3541 acaacaggct gtttgtctaa aaaacggtgc atcttttatt tttttgtacg ttccgggctt
     3601 ttccatgtcc cgcttccata acctaaaatc gttttgactg ctggaatgaa agtcgtcacg
     3661 atcgttatcg cgttcactat ccagaacaaa agcatatata gtggtgcgaa aagaaggtat
     3721 ttcaattttc ttcgtcgatc atctacaatc agcgaagcta gcaactggaa aaatcctgcg
     3781 accatttcaa agcagatgaa cacaaaactg aatgtaaaag taatataaat ctgttcgtag
     3841 tttccttgat atacataatg cccgattaat ccagcaaaaa gaactacgga aatacaaaag
     3901 aatattgacc agacaatact caatgtttga tcaatgaaga tgatcgttct tccaatatgt
     3961 ttaaaaggat ggagcatcac ttttttgaaa ttcgtcagcc agacttctgt tccacctttt
     4021 gcccaacgtt ttctgtgtcg atatagcatc ttcaacgtca caggtacttc catgaaaaag
     4081 ataatctcag gagcaaaaac agataaccag tcatttaact gatgatccca agcaatactg
     4141 atgtcttctg tcgctcgatc ttgtctgaat ccgcccacat cgatcaaggc ttcttttcga
     4201 tacatcgtat ttgctccact gtacgcataa agaccaccga aaacagctga ctgtgttcgt
     4261 ttaatgatgc ccacgatact tgagaattca accgtttgag attttgcaat caatttggta
     4321 cgattttgga catccatatt tgcagtgacc gcagcaatat gtcttgcacc agggcgaatg
     4381 aaataattca catacctaat cagtgcatct ggctccggta ccgtgtctgc atcattgctt
     4441 aaaatcaatt tcccttttgc aaaggcaagt ccgatattaa acgcgtgggc ttttccttta
     4501 tttttttcga tccgaaccac acgaagatta gcgtattttt tcatcagtct agctaatatt
     4561 tctggtgttt ggtccgttga accatcgtct gtcactaata cctcatagtt gtgataattc
     4621 aatttcgtca tcaaatactc gattgtatat tcgatcacaa tttcttcatt atgggctggc
     4681 accatgatcg tgatgaacgg ttcgacactc aagggaacat ctacccattc tttttgctta
     4741 tgtttgtata aaaacacata acaccagaca ccaataaacc atacaaatcc gcctaaaatc
     4801 ggataagaaa cgagaaaaaa atacataata gacaaacaag tatctaagaa taaattacct
     4861 gtatgggtga ttgagatcat ttcagttcca ccccattttc tttatacaaa tctgcaatga
     4921 atgtcgtatc tagatttttc tcttcggaga cagaataaaa acggaccgtt tctctctctt
     4981 cttttgttcc aaaacgttcc gtatacactt cttcaagtaa ttcttgtctt tttttcaatt
     5041 tttcatcttc atattcaaaa gatttattta cttttttgta tagatttcga ttgttccacc
     5101 tagtcaatac aaatgaggta atcaggaaac tgaaaaaaat caatacaaaa aagccgatta
     5161 agaaatacag cattttcttt tctgataaaa aagcgtacaa ggaaaaatcc ttggctcttt
     5221 ctggtctcgt taatgggaaa aagacccata taaaaggaag aacaacgatc atccatccta
     5281 aaagagccac taacgtttgc caaatcttta accagtaatt accttttgaa aaatacttat
     5341 ctttcaccaa aattgatgca acttcttctc ttgttacttt tttcatatcc tcacctcacg
     5401 tttttttgtt acgacgtagg agtttaccat acatttcatt tcttttcaca taaagtgttc
     5461 ctaaattttt cctaagaaaa agaacaaaac atttctcatc agcaaatgaa aaatattttg
     5521 ttctttttat cagttgttct ttttattttt tatctcttaa ttatgttgct ctttccaata
     5581 cgacaatgct tttcattcct ctagatatcg ccgtgtacat ttgtctgcgt cttctttgct
     5641 catctccatc gttttcaaat ggatataaga ttacatgatc aaattccagt ccttttgcta
     5701 gactcaaagg aatgatttgg cagttttcta atccatatga atttttttta atcgattctt
     5761 gtagcgtttg ggtttcctct tctgtttggg tgataactgc cgctttttct ccttttagag
     5821 aaaccaaaat ctcagctagt attttcaaca attcttcaga ggatcgaaaa gagcggtatt
     5881 cgggttcttt tccttgcggt cggatcgata cgatgtcgat cttctcttga tccacagcat
     5941 aggttttgaa aagctctgtg attgctccgc tagaacggta actatttcta agatcatacc
     6001 gatggatcgg cagatggtag tcgtcgaaac aacgcataat atcagcaaaa gtagtggaag
     6061 aattaaagat tgcctgattc tcgtcaccta ctaaagtaaa gtgagttttt ggaaataatt
     6121 cgatcaatag acctaattgt gccagggtat aatcttgtac ttcatcaatc aatagatagc
     6181 gaaatgctgg tacatttacc ttttcaacaa gtaaatgacg aatagctaat ataatgactg
     6241 cttcatcaac agtacgtgtg ggttgccaag cataagctga gccatatcgt ttttcataaa
     6301 ttctttcaaa caactgatgc tcatccaccc atgccatttc ttcgatttgt ctggttattt
     6361 ttcgatattt tttttgtaaa agtttccttg cataagctac aatactttgt tccgaatcat
     6421 cagaaatcaa tttaccgaat aattccaact gcctatcttc tgtcaacgag agtacctgat
     6481 tctgcacagc aggacttttt gcttgtttca aaagatgatt cttccattcg ctcatcagtg
     6541 cctttttcgt tgcttgaagc ctatcgatca atttaggatg agcaggaact ttctcatata
     6601 ttttttcgat cgttttttta gaaaggactg tttttccatt tttagtaagt ccacggataa
     6661 aattcggatg atctaaaaac aaaggatctg attgttttag attatcgata aactttttgc
     6721 tacgcaaacg ttctgtctgc tccgatacat tttctcccgt gatacgtttg aaataagcct
     6781 cttcaccttc tatttcctct gctgaaagct gtgaaaccag ctgtatgatg gttagattca
     6841 gtggatttct ttcacctaat gatggtagga cattcgaaat atagtcgata aaccgatgat
     6901 taggtgatag aatcagcaag tcatcggatg tcatcgtatc tcgatataaa tagagcagat
     6961 acgcaatacg ctgcatgata gcagaggttt taccacttcc tgctacgcca tttaccaaga
     7021 tattttgact tttcacatcc cgaatgattg tgttttgttc cgattggatc gtagcagtga
     7081 tagccttcat ttcattggtc tcatcttggc ctaagacttc taataatacg tcatcttgaa
     7141 tagctacaga cgtatcaaaa aacttgacca aacgatctct ttccaaaaga aattgtcgtc
     7201 tttctttaat aaccgtatcg attttatttt gacgaaccgt ataagaagat ggaccaatca
     7261 tgttattgta aaaaagttcg gcaattggtg aacgccaatc ataaacaaga ttttcatctg
     7321 catcatctgt gaaatttgct gtaccgatat aaaaggattc ttttggctca ttatccagaa
     7381 agtctacaac gacttttcca aagtagggac ttttcaataa acgttcgatc tttgctagct
     7441 gattttctgc gtttttctgt ttgatattca tctgatcgat ttctctattt ttcatttcaa
     7501 tcatcgaaaa agtatccagg ttatctaagt aactgtcaaa attcaatcga agctcgccag
     7561 ccatttcttt caacgactgg tttccttcta tttttgccgt atccatcgat tcccgcagtt
     7621 cgtttttttt atcaattagc ttttgataca cgttcttcag atatgcttct tctaactgtt
     7681 tttgtgtgtt gaccatacac attctcctct ttttttaaag tggtggaacc gattagttta
     7741 cctcttttaa gagtcgaatg caagcttttt aacgagtatc atttgttagg atatatcagt
     7801 tcttcaggtc ggacagtttg atataaagaa aatgcaccat caaggttttt tacgtggtag
     7861 ccagactgct tcaagattct ttccgctaga tagctacgta atccgctatg acagctgacg
     7921 atatattctt gttggctgtc taattcattt aatcggtccc gaagttcatt caaagggata
     7981 tgtttggctt ccttgaattt gcccttttcc aattcttctg ctgttcgcac atctaataaa
     8041 atttttcctt ttgctagttc tgtcggcaat tcatgccatt gtacggtttc actaagccct
     8101 tctgcaatgt tcattgctgc atatcctagc atgtttactg gatctttggc tgagccaaat
     8161 ggcggtgcat acgtcaattc taattccggt aaatcgaaga tcgtcaaatg ccctttgatt
     8221 gctgttgcta aaatatcgat ccgtttatcc acaccttttg ccccaacacc ttgtgcacca
     8281 taaatctcgc cagtctctgg atggaagatc aacttcagta aaatatctgt tgcttctgga
     8341 taatagcttg catgatcttt tcctgataca tgaatgactt caaaagacag ttcgttttct
     8401 ttggctgttc tttcacttaa accggtggat gcggcagtca ttccaaacgt tcgtataatt
     8461 gctgttccaa taccgccttg atttcttcga gaaattccgg aaatcgtgtc cgcaacttgt
     8521 ctaccttgac gattggctgg tgaagcaaga gaaataagtg catcttttcc agtgatttgc
     8581 tgttttacaa cgatagcatc ccccaccgca taaatatcag gaatattcgt ttgataacgt
     8641 tcgtctacta ggataccccc gcgtatgcct aattcgattc cagcggtcac tgctagctgg
     8701 ttatcaggac gaacaccaat ggataaaata actaaatcag cagggatggt acgcccatca
     8761 tccagtcgca atcgtttccc gtggtcttca aatccagcga cagcatgaga agtaatcact
     8821 tctactcctt gatgagacaa ttctttttct ataaaagctg ccatttcttc atccagtgtt
     8881 ggaagaatat gtggtgcttg ttcgatcacc atgacttcta aacctcttct ttttaggttt
     8941 tctgccattt ccaatccgat gaatcctgca ccaacgatca cggcacgctt tggctgtttt
     9001 tctaatgcat gcatcacttt atcgatatct ggtacatttc taatagaaaa aacattgtcg
     9061 gcttctgtta tccctggaat cgatggaaca actggttttg caccaggaga taaaaccaat
     9121 gcatcgtact gttcaatgtg tttttgaccc tcatgtacga cttctaccgt tttgttttcg
     9181 ggaaagatgg ctgtcacttc atgatttgga cgcacatcta aacaaaaacg agcggataat
     9241 gattctggcg tttggacaag aagattttcc cgttcactga tttctcccga aagataataa
     9301 ggcaagccgc aatttgcaaa agaaacatac ggtccttttt caaaaacaac gatttctgca
     9361 tcttccatta atcggcgaag ccgtgtagca gcagacattc cacctgctac acctccgaca
     9421 ataacgattt tcatcttatt tccctcctac tgttcgatca aaccattgat tcatgccacc
     9481 acgtacattc actacatcat aaccagattt ctttaattct ttagctgctt gtttactcct
     9541 catccctgat tgacaaatca catagacggt cttttctttg tctccatcat atctgtttat
     9601 actttgtaat ggaacatttt tagctccttt tatatgcccg ccccggtatt ctgcaggtgt
     9661 tctaacatca agtaacgctg gattttccaa aagcttttcc tttaattctg ggactgttat
     9721 cgattttatt gattgaaata gcaaattcat tttctgcacc tcatttacat ttttataaat
     9781 acctcatggg gtatattaaa caggagcttt attaatatgt caaccagaat gactcttcat
     9841 ttttttcact actttttata tttgcataaa ataccgataa ggggtattat ctaattataa
     9901 aaatagagga gaatgtgtta tggagattga taataaaaat atcctgaacc gtttgaaacg
     9961 tacagaggga caaattcgtg gtatccaaaa aatgatcgaa gacgaaaaag aatgtatgga
    10021 tgtcatcact caattaagcg ctgttcgttc tagtattgat cgcgtaatgg gcatgatcgt
    10081 tgcagataat ctgaaaaatt gctttgagaa acctgatagt aatccagaag aacaagcggc
    10141 taaactagaa caagcgatca agatgattat taaaaagtaa aggaatgatt tcaatgaaag
    10201 tcgttttcca tattgatgaa ttagaaaaat ggtcagaaac agggaaaaac gtaaaaaacc
    10261 tgataaaagc ttctccagaa acgacgattg tcgtatctgt aaatggcatc gcgattaccg
    10321 gttatctaga ctctgccaat gcagaattct tagatttgca aggagtcgtt tttcacgctt
    10381 gcgccaatgc tatgcgtgct aatcatattt cagaaagttc tttacctgaa caagtaatcg
    10441 ttgtaccagc aggtgtcctt gatttagttg aactgcaatc ccaaggttat gcttatatca
    10501 agccttagtc agtagaggtt gcgacagaag tgatcagctt caagaaattt cggcttattt
    10561 ttcgaagaag ctacttctgg aaaaccgtga atcaaaggtt gtgaaaacgc tgtcctgcat
    10621 caagtaataa gaacagccaa acaaaaatag cttctcatat ttttcgtttg gttgggctta
    10681 ttaccgcaga tgcaagcttt tgaacaccgt ttaaggaaga ggttgtgaaa tcagcggttt
    10741 gacctaagta ttagagaaaa aaacgggaaa atagctcctc atattttgcc gttttttgag
    10801 ctaataccga aggtcagctg attgaacacc gtttaaggaa gagattgtga aaaagtcgct
    10861 tagtttcgag tattaagaaa aattttgtga aaatggattt ctacattttt gcataaattt
    10921 ggcttaatac cgagaaactg gcttttgaat accgtttaag gaagaggttg taaaatcagc
    10981 ggtttgacct gagtattaga gaaacaaacg gaaaaatagc tgctcatatt ttgccgtttt
    11041 ttgagctaat accgaaggtc agctgattga acaccgttta ccaagaggtt gtgagcctac
    11101 catttaactc cgaatcacaa gaaataattt caaaaacaac ttctcctgtt tttggttcgg
    11161 ttggcttatt atcgagaagt agctactggg agtctattta tcaaaaaaag gaatgcgaca
    11221 taggtcacac ccctttttta ttttctttga aacatctttc ggtactcgac agcatacaag
    11281 acaaaagatt gtaaaagacc gtaagtgatg acggcaaaaa tgactctcac ccacagcgtt
    11341 ggtaattctg ggaaaagcag caaaaggatc atgccaacat agatgacagc agtcgctact
    11401 ttgccatacc acttcgctcc acttaatttg atatttttag atagcccgat taatccagct
    11461 acaaacataa agccttcttt tatcaagaat agaccaaaca acagccaaag ccacggtcga
    11521 taccatgcca tagacaaaat cagaaaaagt tgtgtcaatt tatctgcaaa cggatcaagc
    11581 accttcccta agtctgttac ctgattatat ctccgagcaa tgaccccgtc tagataatca
    11641 gacaaacctg agacaacaat gaccgctcct gctatataaa atgactgtct cagcacaaaa
    11701 taaagataaa tcggcagcaa aaaaatacgg aatatcgata aaagattcgg aatcgtttgt
    11761 atttctttac gccagtctgt gcttaatttc attttatcgc cctacctcct cagcactctt
    11821 ttctttttat tttagcttac acggggaata aaaccaatga tttcactgtt tttatttttc
    11881 tttacagaca ctgatattat gagctaattt gagataatca cttgctgttt tcatcgttta
    11941 ctcttctttc ttcctgtcgt ttgcgcattt cttttactag tactttatga gctttttttg
    12001 atcctcgctc taggtcacgc tgccgtttac gtgttttata atcgataaac agactgtcta
    12061 aatcgtaacc tgtcgggtac agatcttctg ctgaacgcac cagacgaaca cgctgacgag
    12121 gcacgtggat tatttttttg tctaaatata catcaatcgt atcttcacct atatcttcat
    12181 aaactaacgc ttctttttgg tattcattga caaatatccg atcacctttt gcaaagagct
    12241 gacgttcttt tttattctcc ttcctatttg taactgctgt cttaggaaga aattcttttt
    12301 tctgtgtccg ataggctttt tccgccaagt aacgctctgc ttgatggatc aatttcatcg
    12361 acatatccat tttatgagca atccacaaag cttggctttc tcctgtttct tccaacccgc
    12421 aactgatatt taggacgaag tgcttcccga tcgaaagcca tagcagccgg cacaaaatct
    12481 tcatgatctc cggcgaattt cttgatttct ccataatggg tcgtggcaat gatcaatgcc
    12541 ccttgctcat acatagattc catgattgcg atcgctagtg cagccccttc atttggctct
    12601 gttccactgc caatctcatc taaaagtacc aacgtattcc tttttatctt tttcaaaata
    12661 gcagcgatgt tttgcatatg accagaaaat gtactcaacg cattttctaa attttggtgg
    12721 tcaccgacat cgacgaaaat ctcatcaaag acagcaagct ctgtcccttc ttttgccgga
    12781 acttgcatac cgaacatcgc catcaaagtc aataaaccga ctgtcttcaa cacgactgtt
    12841 ttgcctccgg catttgctcc ggtgataatc aagcctcgat agtctttacc gatttcaaca
    12901 tccaatggta ccgcatgctc aggtaaaaat ggatgtctgc cttgcttgat aatgatatgt
    12961 tccgatttat tgattctagg tgtgcttcca ttgatttctc ggctaaattt tgcccgagca
    13021 aagatgatat ctaacaccgt gatcgtttcg atcaataaat cgatcacttc ttcgttttct
    13081 gcgatagcac cagtcaatgc cgccaggact tgatattctt cactgatttc ttcagctttt
    13141 aacaactgat agtgttcatt caatttagaa atcacagttg gttcaataaa aaccgtcgtt
    13201 cttttatttg attcatcgat aatcgttcca tctattttat ttttatagct ggctttgata
    13261 ggaatcgtat agtattcgcc tttttgaaca atcattgctt cttggatcat ttctttgttt
    13321 ttcggatgac gcaagaattt cagtaatttt gactggattt ctttttcgat tagttgtaat
    13381 tgtttgcgta ctttacgcaa attacgtgac gcgtcgtcac tgactttatt attttttatt
    13441 ttctggtcaa tcaactcttc tacatttatc aaatcaggaa gatgtttgct ataagcaaac
    13501 aacaagggtg tctggtattg attcttatca aaaaactttg tgatcatccg actgcttcta
    13561 agaaaatccg catattcgat caaatcggtt ggctgcagaa tcaacccttt tttgacttgt
    13621 tctgtcagtg catcgatcct cggcaacccc ataaatggaa catgctgact gctttctaag
    13681 atcaagcgtg cttctcttgt ttcttcttga cgatccaaga cagtttgtag gtttgtcgag
    13741 accgtcatat cgctgattcg ttttttgcta tagtttccaa ttgctcttgc ttggatttcc
    13801 tttttgatcc gatcaaattg taattgacta atcatttcgt tgtccatatt ctctcctttt
    13861 cttgaagaga tacctgtccc aaaataaata agggcctaga gataaataaa aggatagaac
    13921 acagcttaaa aaagtcatgt cctatcctta ttagaataca aagatattgt tttcctgaca
    13981 ggtaccgttt cataagcaca ccaaataaac tttgtttatt ggctattcga cttactgatt
    14041 agataccagc tacactcaca aaacataccc cattcttcta ggtcttattt attttccact
    14101 taactatagc aagacacatt ttggttgtca aactttagta tgttttatag atttacacca
    14161 ttaatgaata gacacctacc gcgataattg ctccgatcat tggacctgcg attggtaccc
    14221 atgaatatgc ccaatctgaa gttcctttat tagctattgg caataccgca tgtgctaatc
    14281 gaggaccaaa gtcccgagca gggttgattg catatcctgt tggccctcct aatgaaagac
    14341 cgatggctaa gatcaatcca cctacagcga atacgttcgt tccaggtgca aattcgtttt
    14401 gtccaaaagc tagcaaccca agaactaata caaatgtacc aattaattct gtgatcacat
    14461 ttgctggata attccgtata gcaggacctg ttgcaaacgt tcctaaaatc gcgctttcat
    14521 ctttcgttgc attccaatgt ggcaaataag aaagccaaac aagaatggcc cctgcaaaag
    14581 ctcctaaaac ttgcgcaatg atgaaaggaa cgacaagatt ccaactaatt gcccctgtca
    14641 tcgccatagc tagagagact gcaggattca aatgagctgg gcccatgaat ccagaaatat
    14701 agacagccat tgttacagcg agtccccaac caaatgcgat gacaatccag ccagaagcct
    14761 gtgctttact tttgtttaaa ttcacagccg cacacacacc atctccaagc aaaactagga
    14821 tcagtgtccc aataaactcc cccatgattt gagtcatatc actgttcatt tatttctccc
    14881 cctttagttc ccgcagatct gattctgcaa tcaatgcttc taaacgttta gtctgttgcg
    14941 ccttctcctc ttctgtccat tcaaaatatc cagcaattgc atcgatcaca ggttgcttga
    15001 tctcatcaag ctgatctcgt tcaaacaaca gatgatttgt acgacgaatg agataatcgc
    15061 ctggggccaa aaccatttct tcttctaaac cataacgtaa ccgagctgac tcagcgagac
    15121 tcaaaccagg ataaggtgcc atttcttttg ctaattcaaa gatacgtcgg gcattagtcc
    15181 cgtaaaaatc agcgatataa gtagcatctt cttcctctaa accggctgct actccttctt
    15241 ttgctaattc tgtcactgtt tcttctaatt tagttggatc gaagtttcca cctgaaatct
    15301 gatatttttt agagtcgatt tctttcgtct ctattccgta ttcttctttt aataattggc
    15361 gtatcaatcg caaagctcct tctgccatct tacggtaatc ggtgattttc ccaccagata
    15421 aagtcagcaa accatctggt tctctttcta aagaactacc tctggaaatc gtagaaggtg
    15481 ctttttcatc acggctgttt tccaaatggt tcaacacgtc ttctacttca gcacgagaaa
    15541 ctttgttttc cttatattca cttactgtat cgacaacttt attgaagctc ttgtctgaaa
    15601 tcgatccatt atctccacca ttgtaatcag aaccagaatt accaatcaac agtggacgaa
    15661 gtcctgccca gcttgcttcg atatcattca atgtgatatt tgcttctgga tagcgatgat
    15721 tgatcacatc caatagataa tctacatctt cttgtgtgac tttcggatcc gtaaaatctc
    15781 cttggtaatc cgtatctgtc gtaccaaagt aagtcttgtt ttctcttgga atagcaaaaa
    15841 ccatccgccc atcttgcttt cctgtatcga agtatgtggg ttgcggtaca ggcagttttt
    15901 tcgcatctac aactaaatgg atccctttag ttggacgcat tttaggagag actggacgcg
    15961 taaaattcaa gtttcgtact ttatctaccc aaggaccact cgtattgatc actaattttg
    16021 ctttgatttc gatcacttca tctgtcagca ggtcacgagc tttgacgcca acgatttgat
    16081 cgccatcata taaaaagcca accgctttca ttttacttac tagataagcc ccatcttcgg
    16141 cagccttttt gatattatca atcactaaac gggcatcatt gttgcggaaa tctagataga
    16201 cacccgcgcc ttttagccct tcttttttca aaaacggttc tctttccaat acttcttctg
    16261 gtgtcagcgt ataattctca tatttggttc ctgtcacatt tgccagtttg tcataaaggt
    16321 ccattgctac tttgaccgag aacatattga aggttgttgc tccttcgccc tcatagattg
    16381 gcaaaagcat tggatctggt tttgggatgt gtggggcaat actttgtacg actgcacgtt
    16441 caccaactgt gtcagctact acttccacat caaatgtctt cagataacga ataccgccat
    16501 gaacaagttt ggtcgagcga gaggaagtcc cttctgcaaa atcctgcatt tcgatcaatc
    16561 ctgttttgat tcctgatgct gctgactgga ctgccacacc ggcaccagtg attccgccac
    16621 cgatgatcaa cagatccaat tcttcttgct gcattttttg aatgctatct tgtcttgtct
    16681 tgtttgaaaa catatggatt tcctccttat tcaaatgctt gggtagcttt tactgctttt
    16741 ttccaaccgt tgtaaagttt ttctcggcgc tcttcctcca tttgaacttc aaatagcttg
    16801 ccagcttcat aaaattcgcg gatctcgtct gtatctttcc aataaccgac agctaatcct
    16861 gctaaaaatg ctgcccctag cgccgttgtt tccaagtttt ctgctcgttg gatcggtaca
    16921 ttcaagatat ctgtttggaa ttgcatcaga tattcattgt ttgctgcacc gccatctact
    16981 ttcaatacag gaatcttgat tccagtgtca tcttgcatcg tatcgatgat atctctcact
    17041 tgataagcaa tcgactgtaa agtagcttta atgatatctt cttttgtcgt accacgtgtc
    17101 aagccaaaca tagcccctcg tgctgcctga tcccaataag gagcacctag cccaacgaat
    17161 gcaggaacaa cataaacctc atcttcgctt gtagagcgtt tcgctgcgtc ttctgaatcg
    17221 ctagcttttt gcaacatttg aagtccatct ctcagccatt gaatcgaaga accagccaca
    17281 aaaatacttc cttctaaagc atagtaaacc tttccattta tgccgtaacc aattgtcgtc
    17341 aataaattat ttttggataa ttgcggtttt tcaccggtat tcatgacaat aaatgaacct
    17401 gtaccataag tattcttgac catgcctggt tcgaatgcca tttgtccgaa taatgcggct
    17461 tgctggtctc ctgccatccc tgaaattggt gcttcgcttc cataaaaatg ataaccttgc
    17521 gtataaccat aaacttctga gttagaagtg acttttggta acatcactcg aggaatattt
    17581 agtaaatcta aaatctcctg atcccaatct agctgatgga tattgaacaa catcgtccga
    17641 ctggcattcg agtaatcagt aacatgtgct tgtccatcgg tcagtttcca tactaaccaa
    17701 gaatcgatcg ttccaaacat caattcgcct ttttcagctc gttcttgcgc gccttctaca
    17761 tgatcaagaa tccagcgtat cttcgttgct gagaaatagg catcgatcac taaccctgtt
    17821 ttttcgtgga aaaattcttg atagccttca ttttttaagt tatctgcaat tccagctgac
    17881 tgtctggatt gccaaacaac tgcatgatag attggacgtc ctgtatcttt ttcccatatt
    17941 actgttgttt cacgctggtt cgttatgcca atcccagcaa tctgatttgg tttgatccct
    18001 gattcgatga acgctccggc aataactgac tgtacagaat tccagatttc atttgcatca
    18061 tgttccaccc aactttcttg cgggaaatat tgcgtgaact ctttttgaga actgccgatg
    18121 tgtcttcctt tcttgtcaaa gataattgct cgtgagctag ttgtaccttg gtcaatcgcc
    18181 atgatatacg ttgattctgt cattgtgatt ccctccatta tatgcaaacg ctttctttaa
    18241 ctactcttat catactactg ttttaaaaaa agaaactgtc aaaattttat tctttttttc
    18301 tttttttgac agttttttat tatgaagcta ttttttaatc aattcttttc ttttttcagc
    18361 gtctctattc ttcttttcaa tcgaataatg tcgtaaggtg acgcaaattc tgacagataa
    18421 tattcacact gttttttgtt tctctggcaa aatgtgatca ccaaatcata ttctttacct
    18481 tcttcataac ttccaatctc aataccgccg attcccctca gttctctgat cagatattgt
    18541 tgaaaagcaa tacgataaat cggcagactt tctaaatcga ttccaatcac tactggtttt
    18601 gcaatataaa aatcgatcat caaaagaaca ttggcatact ggatcatcag gtaactaagc
    18661 tcactatctt cagctctttt cctcggcaat tgctgcaccg ctgtttcctg taatttcgcc
    18721 agtaactgtg ttaaggaatg cccgagcatt ttctgctgct gttccaaaag ccgatcacgc
    18781 tcatagactt cgatctttcc aacaaagaaa aaccattcat tgttgatctg ggaaatttgg
    18841 tagcctactg ccttttcttc tttgattttc aaacgacgag gacgataatg ccataacatc
    18901 gtttcccgaa tgtaagtatc taaaacagcg gttggtaact ttttgctccg tgtcaaatca
    18961 tactgataaa atatttcttc gtctactatc gaaaaagaaa caaaaaagct gtaaaacatc
    19021 attgtttcgt atacattcag ttctgctgct gttcgactaa gatgcatcac gatgattgga
    19081 tcgattgctt gataaagccg gtccgactga tagagtaatt tcttttcttt caaagttgcg
    19141 tacgtacttt tttcattcaa tagtcttttt ttcgtaatcc cgagccagca agcaatctgt
    19201 gcttctgctg atgccgtaaa cgttgtttct aatacacggt ccaatcctag aatgaacggt
    19261 cttttttctg gtgtattttg caaaaaaagc ggtcgctgat cttccgggat atattggaat
    19321 agttcataat aaaaatatcg tatctgtagc tcttctccgt ataattgacc gtttttgatt
    19381 tgtaatccaa attcctctaa gagctggttc aattctttga tctttcgaaa aacagaagac
    19441 tcactgatat taaacgctgt cgctaatcga acgatggata cttgctgatg ttctaataaa
    19501 tagacgagca tcttaaactt caagctttcc tgcaataaaa acaatagaat tttttccaaa
    19561 cttacatctt cgtccaacac aagcagcgcc tgatactcat ttcgctgtac ttctactttt
    19621 ttttccatca ttcggccaat caattcaata tcttccaaat attgatcaaa cgaggctttt
    19681 gacagttgga catgctctct caaaccattg atagaggctg ttcccccttg cagaacaagg
    19741 tttcgcaaaa tcaggatctg taactgctct tttctttcta ataatgcttc aatcttcata
    19801 ggcagtattc gccacctttg accacaccat atctggcaca cggtattctg gctggaaaaa
    19861 tagctcatgt tcttctaact gttcaaacgt ccatccttct tctactgcaa ttgtcagttg
    19921 gtcaagaagg tggaacatct cttttttgct ttggagctgt gctccaagaa tctttttttc
    19981 agaatcaaat agaaatttga gtttaacttt gctttgattt attgctgtaa gcttgtagtc
    20041 tacgtatcct gtttcaattt tttggggata aaaaatgctc tcttcttctg tcaaacctac
    20101 gcttcctaag aaatagccaa accactgatt gccgactgga cgataggttc cctgatcctt
    20161 tgtctgtgag ccactaattg tcttgctgac atttcgtgct gtacggacag cgttatttac
    20221 tagagaaaca tacatttttt catttgttgg acgaaaagtt acttggatcg catcgccaat
    20281 cgcataaacg tctttttgag acgtttgcaa gtagtcatct acccagatcg ttccatcatc
    20341 attcaatgtt aattgttctt cccacatctg attgtcggga cgagaatgat tagccagcaa
    20401 aacaagatca tagttctcct ttcttttttc cgtctctaca atagcggttt cattttcggt
    20461 caaagacaca acttgttctt ctaaaaaaag tgaaagccct tgatttttca tttcctttac
    20521 taacggttct gtcatttcag gatctagata acgaaaaaga attgactttc ggctttcgta
    20581 aagatggatt tgtttttttc ttgatgccaa cccttcagct atttcgattc ctgcttgtcc
    20641 tgcaccaata attgcaattt tctttgcatt catgatcttt tgctgaagag ccgttacgtt
    20701 ttgtatcttt ttaactgggt gaataagcgt tgattctgca gaaatatttc gaaacttttg
    20761 atttgaacca gtcgctagaa tcaaacgatc aaaatccaat ttttttcctc ctgctaaaac
    20821 gacttttttt tctttcaatc ctattactgt cttttctgtg taaacatgga ttcgatagcg
    20881 gtcagtcagt tcttctttcg tgatccaaga aagttgtttt tcactggtaa cctttccttt
    20941 taatatcaaa ttgaacccgc tgggaatcca tcctaacttc gattgctttt caatcaaata
    21001 tatttcactt gttggcatca attggcgaag ataaatagcc gcatgaatcc ctgcaaacga
    21061 cccgccgaca attacaatgt tcatctgcat tcctctttct tataaggtgt tctactgcta
    21121 acttctcaat cagaaaacac agtattcata actcgctttc aaccttatga tacatagcga
    21181 tgcagcagaa tcaaccctct ccatcatacc gtgttctagg tctgcttctc aataatgtac
    21241 tagtggacct aattgaagag tatcatttct gaagagatgt ttcaaaaaat gtactcttat
    21301 aaaagaaaaa aactcgcctt ttacgtacct ctgtccaaat aggaaacaga aaagaacgaa
    21361 aagacgagtt ttagcgtgtg taatgtaata gttagaatag acacatcatt tatttttaat
    21421 tctttaatgc ttcaacaact gcttcgccta attgatgagc agattgagga ttttgccctg
    21481 taagaagccg cccatcaatt tgaaccactt ctgaaaaagc tgctccagac tgatagtttg
    21541 ctcctttttc tttcaaagca tcttctgcta agaatggtac ttcattcgtt gtaccatttg
    21601 cttcctcttc ttcattactg aacccagtca ctgtacgtcc agctaaaata gattttccag
    21661 actcatctgt aactggcaac aatcctacaa caccgtgaca aacagctgag accagccctt
    21721 catttcgata gatttgtgaa gcgactttcc caagatctat tgcttctgga aaatcccaca
    21781 ttgttccgtg gccaccagca taataaaccg ctgcataatc atttgcatcc acatcatcag
    21841 gacgtaaggt atttcccaag gcccgatttc tgaaatcttc atcttcataa taggcccaat
    21901 cgatttcatc catattcaaa acactttgcg gttctaaagg tacatatccg cctttaggac
    21961 tgacaaaatc tgcatcgatc ccatttttta ctaatacatc ataaaaatga gtcagttcac
    22021 ttaaccacaa acctgtagga cgctcgatct tttcgtactt ttctacattt gttaatacga
    22081 tcaatacttt cattttccca tcctctttct ataaaaattg cgttattgat aacgtgcttt
    22141 caagaaatta tataatgggc ttggcaaata aagctcttta tatgcttttt caaaaggttt
    22201 cgttacaaat tcatcctcac ggttttccaa taatttctct gcccagtcag gatcgacgat
    22261 catttgctgt ccaattgctg caagttctgc atatttcaat acctgctcta catcttcgcg
    22321 attgcggatc ccaccgacac caactaaagg gatctttcct tgaatcgtgt tatggaccac
    22381 ttcaagaatc gatttatcgt tgtatttatc agaacgagct attcgatcat acatatttaa
    22441 tgaaacatgc agataatcta gttttgactc acgtaatcgt tcaagaagcc agatcgtgtc
    22501 ttcaaaacgg atacctggtg tttcatattc ttctggtgag aaacgatacc ctacgatgaa
    22561 tggtttttta gcatattgtt caaccgctga gaaaaccgtt gttaataatt cttcaataaa
    22621 acgataacga ttttccaagc taccgcccca ttgatcctct cgtctattcg aatgcggaga
    22681 gaaaaattgt tggatcaaat acgtatttgc tccatgcaac tcgattccat caaatcctga
    22741 ttcaatcgct ctacgtgtgg ctttgccaaa tgcttggatt gtttcaacga tttcttcttc
    22801 actcattgct cgtggtgttt ccgcatcagg acgttttgct gcaactgcac ttgcagatac
    22861 aggctgttct ccagataaag ttgctcgact tgtcattcgt ccaccgtgga aaatctgaat
    22921 aatcgctttt gcgccttctc cttggattgc tttcgctagc tcacttaatc gaggaatcat
    22981 ttcatcatga gcgatgctga gttcacctgg ccagcctttt ccgatatctt ggacatttgc
    23041 agcaccagta ataacagcac caagaccttt agaacgttgt ttataataag tgatctcatc
    23101 ttcggtgatc actccattga aaaagctgag ttgtgtcgtc ataggcgaca gcatcaatcg
    23161 gttcttcaat tgaactcctg aaggtaaagt aattgattga tcaaattgtg acattgtcat
    23221 tcctcccata attcgctctg attaacctat cgagaatcat ctatcattaa accactcgat
    23281 tagttgtaca tgcaactaat ttgcttaaaa aattttattt tttttgtaac ttatctgttg
    23341 catcattcat tgtctgggct aattctttac ttgcttcatt tcccaaaact gctttgtatg
    23401 actgatgaag ttcttcccag ctaccatcga cttccttagc aaattggcgt ccttgttctg
    23461 taagataaac taatgacaag cgaccttcgc taactgtttt gacgtatcct ttatattcta
    23521 atttttcaac aaaacgagca atcgttgatg gggcggaata gagcatttgt gcaatatctt
    23581 tttgtggtat tccatctttt tcttttaata tcaataaaat aaaaccataa gttggtgcca
    23641 gacctgtcct tctaaacgct tcatcagcta atttattcaa catgcgatct agctttttca
    23701 cgctaaagaa aagacattca tctaacgctt tttcaattgg cttttccatt tcatcccctc
    23761 cattggcgta catatcatag tatgattttt attttttgtc aaaaaacacg gaataatgtc
    23821 cgcctcctct gtctagattt gaaaaaaaga agtagccatc ttcaaagaat ttcggcttgt
    23881 tttcgacaaa actccttctg gaacaccgtg aatcagaggt tgtgaaaaag tcgtttagtt
    23941 ttgagtatga agaaaaattt tgtgaaaatg gctttccaca tttttgcata aatttggctt
    24001 aataccgaaa aactggcttt tgaacaccgt ttatatcaga ggttgtgaaa aagccgtcct
    24061 gcatcaagta ataagaacag ccaaacaaaa atagttcctc atattttgtc gttttttgag
    24121 ctaatgccga tggtcagctg attgtacacc gtttattgag aggtcgtgag cctgccgttg
    24181 aactccgagt cacaaggagc aatttcaaaa aacagcttct catgttttat gactatggtg
    24241 acttgtgatc gaggagttgg caggagaata ctgtttattc gtaaagaagg ggctgtgaca
    24301 agacttttgt cacagtccct tatttccttt tattatagga tttcgccatt tgattggatg
    24361 acttgtctgt accaatcaaa tgattttttc tttaaccgtt tctttgtgcc attcccttcg
    24421 tcatcttgat caacatagat atatccataa cgttttgaca tttgtgaagt agaagcgctg
    24481 acgatatcaa tgcagcccca cgacgtataa ccaaataaat ctactccatc tttgatagct
    24541 tccttcatct gctcaatatg ctgcttcaaa tagtcaatgc ggtaatcgtc ttgaacggtt
    24601 ccatcttctt ctactttgtc tatagctccg agtccgtttt cagctatgaa cagtggtttt
    24661 tgatacctat catataattg attcaaagca attctcaaac ccttaggatc aatctgccag
    24721 ccccattcac ttgatggaag atacggattt ttaattcctc cagttattaa gtttccttgg
    24781 acccattccc aattttctgg atgagcactg actgcactag acatgtagta gctaaaagaa
    24841 acaaaatcaa cagggtaggc tgctaagatt tcttcatcac ctacttctat ttttaagttg
    24901 acaccttttt cttcaaacat tcgttgcgta tacgctggat aatatcctct tgcttgaaca
    24961 tcactaaaga acaaagaagt tctttgctgc tcaattgttt tttgaatatc atccggatca
    25021 caagaatacg gaacaagaag ctgatagctg atcatacaac cgacttgtga acctttgata
    25081 atttcatgac aagcttttgt agctaaagca cttgctataa actgatggtg tgcaccttgg
    25141 tatttcgctt gttccaaatg gggatggttt tcttcaatta ctcctacgct aacaaaaggg
    25201 tgatgtttta cacagttgat ctcattgaaa gtcatccaat attttacttt atttttatag
    25261 cgtttgaata ctgtttcagc gaaacgaacg taaaactgaa taagctttcg attaggccat
    25321 ccaccatatt ctgtaaccaa atataacggc atttcataat gggaaagaga aacgactggc
    25381 tcaatcccat attttagaca ttcgtcaaac acttgatcat aaaaaaataa tcccgcttca
    25441 ttgggtgttt cttcatcacc atgaggaaaa attcttgtcc atgcgataga catccgatag
    25501 catttaaaac ccatttcagc aaataaacgg atgtcttctt taaagtgatg atagaaatca
    25561 tttccgtgtc ttttaggata ataatacttg ctgttgggat cctgcgccgc ttttacttgc
    25621 tgatgcgtca tcatatgcca ttgatctgtc catttttctt taggtaaatc gctgtcgaat
    25681 gtatacacat cagacacaga taatcctttt tcatctacat cccatgcacc ttcacattga
    25741 ttggccgctg tcgctccgcc ccataaaaat ccttctggaa attttttcat atcgatcccc
    25801 ctaaacttgc tagttttctt gtttgattat ttttcttatg actagtaatg atatttttga
    25861 atctactact atttgtaaca ataaccattg tggtcggatc atatccctct ttgattaatt
    25921 cttgcaaatc tacttccatc aataaatcgc ctttacgtac tttttttcca tcagatactg
    25981 cactttgaaa gtatttgcca tttaaattca ctgtatcaat accgatatgg atcagtatct
    26041 ctgctccttc ttcactgact accccaattg catgtttcgt tggaaaaact gttgaaactt
    26101 ccccgtttac agaactgtag atttttccgt ccgttggaat gatcgcaaac ccttctccta
    26161 aagatccact agcaaatgtt ggatcgttta cttcagataa atcaattatt tcacctgctg
    26221 caggagtact taacactata tcctttgagg attcttcttc gatttcatcc ttgtagccga
    26281 aaagaaaagt tagcatacag gaactgatca tcgctaagaa ataactagca actagccaaa
    26341 caaatccttc accaatataa atcgggaaag acaaaatact tcttgtagca actgccaaag
    26401 caccggctcc tgcggcacca gctactccac caaatatagc tccacatata caagcaatgt
    26461 aaaatggtcg tttatattta agagtcacac cataaacagc aggttcagtg attccaaata
    26521 atgctgtgat aaatgcagat aaagataaac tttttacttt cttatttttc gttttcaaaa
    26581 ataccccaag tactgctcct gcttgcgcag ctacggcagt catacatgct ggacctagcg
    26641 tatcacgacc gtagaaagaa agattgttgg tcattactgg tacaagtccc caatgtaacc
    26701 caaaaatcac taacacttgc caaaatccgc cgataacagc accagataaa acagggctaa
    26761 agttataaac agctaaatat ccagctgcaa tcagctgact tgcataatct gcaattggac
    26821 ctacaactaa taaaataatt gggaccatca cgatgataca aatgaatggt gtcaataaat
    26881 ttcgaatact ttcgtgcaag cttttgcgaa tcttaggctc aatataagaa agaacccata
    26941 cagctaaaat tgctggaata accgtacttg tataccttgc tagaatcacg ggcatcccta
    27001 gaaatcgcaa ggttatgcta tcactatatg cactgacaat cgtaggataa acgagagctg
    27061 ctgcaataac catagcgatg aaacgatctg tattgaactt ttttgcagca gtatatgcca
    27121 aaaatatagg caagaaatag aaaaatgcat cagctgctgc attcaaaatg atataagttc
    27181 ctgattcagt agttaataaa ttcaaactcg tacataaggc gagtactcct ttgatcaaac
    27241 cacttcctgc caagacgcct aaagtcgggg caaaaacagc ggcgatcatg tctataaaag
    27301 agtttaaaat gccttttttc tcagttcctc tctttccact agatgcatca tcttctagtt
    27361 tcatttgttt ggataatacg tcatacactt catttactgc atttcctact acgacttgaa
    27421 acattcctcc agcttcgact acagtgatca caccttctag ttgctctacc ttctccttat
    27481 ctgccttctt attatctttt agggtaaagc gtaaccttgt tgcacaatgg taaacattgc
    27541 tgacattttc ttcgcctccg acaaatcgga caatatcttt cgctaattgt gaatagtcca
    27601 tttttacttc ccccttgtta attaatgccg tagcaatcct tcttacaaga taagtatagt
    27661 tcttagaaaa cggtttctcc attcaattag taccccttct aatagcgaac gaaactgtac
    27721 aatagtgtgt atggtttact tagccgagca aatcactcac tgcttgaaag aaaacaagta
    27781 tatcgtttct cttatagtca aaggagacaa aaaaaacacg cttgatcttc ctttgctgat
    27841 gtataagtgg tatctcaagc tgatactctg tgatttttgt ttctacttgg cttttgataa
    27901 gacagcacaa caaagtctcg gcatttaacg gaagcataac taggtcagaa ctaggagaga
    27961 ttagttgctg aatggaatct atctttttat ggatttttat ttgattttct tctttgtagt
    28021 aatgatcaag cagttttgtt ttcttttttt cttcaactat ggtcagcttt tcacgcaaaa
    28081 tctgataaat ctcccaagat tcaaactcat ctagaacact aggtagataa atcaaatcat
    28141 cccactcaaa aataagctct tgctcagtag tcaaaattgc atcatattcc gttattttgt
    28201 ttgattgatt tttgaaaaga tacactggtt ctatccggca ttctttgatc cacatgttca
    28261 tactttgttg aatctgtttt tttagtgtat tcaaaatact aattggctgg ttactaacaa
    28321 tcagtatctc cccttgtttt cgaaaaggat tcaacgcttc ggcaagatac aaaacaaaat
    28381 aagcgctttc agctaaattg ggacgtaatt gaaaatgtat tgggaagaaa cgccgaatta
    28441 aataaatttc taaaggataa cgggagatga tcttttgtac aaaatgattc atggtattat
    28501 gtccggcatc catcctcgtt ttcatttgtt tgatatgggt tagcaaaaat tctgtttcat
    28561 ggaacaataa cgaaacagga agatctaaaa aacgaataat cgtttgctca aatgctctta
    28621 gattcttctc tatttctttg tttttttcat cattaatcat caaataattt aactctaata
    28681 gtcgattttc aattgcttgt ttttcttgat tggaaaaaga atagggtagt tctgatgata
    28741 acttttttgt gagggtctct accatcggaa taaataatgt atacctgttt gttttttcta
    28801 aaagttcttc tcttcgaaga atggtcaaaa cgagataccg agaaaatcgt atgaagtcct
    28861 ctcctgaaac aatataatta tatgatataa gaacctcttt taatgtcttt tggacagtcg
    28921 gcatgatatt ggaaaaaatt gtactgattt gttgaggaaa ccttgcgtgt tggatcaccg
    28981 tttctgttcc aacaagagat aagaaaattc tttttgaatt ttcttcagca atgattttta
    29041 gtccttttgt cgacgaaact tccagttcta atttaggatt gcgctcgatc caacgcaaaa
    29101 tcgtttttat ttgtaatgat acggctgttt ttgaaagaaa gaaaatgtct gctatctttt
    29161 gcatactgac ataccctttt tgaaacaata agtacgtcac aatagttgaa gctcggaaat
    29221 ccatgctgga atagatttta tgtttcgtca cctcttgtat gatttgctgt ttagtttcag
    29281 attcaattga ttctaaaaaa taacctgaac gtttatttga ctggataatg attttcttct
    29341 cgtagatagc aagcatattt ttgatttcag tttggatcgt tttcttgttg gtacctaaag
    29401 cttcagctaa ttcttcgctt gttatccaat gattcttatt tcttaatact acaagaattt
    29461 ggagttgtcg tttatttaag tccatttgct tacacctcct ctttgttata aacatacagg
    29521 aaaaacctct caaatgacaa tagtcacttg cagaggttta aaaatcattt gaagaaagat
    29581 ctatattcaa ggtttttacc attagactgg ataatttgtt tataatattc aaaactatct
    29641 tttttcaatc tcctaaagct tccgctccca tcattttgtc tatccacata aataaagcca
    29701 tatcgcttcc tcatttcccc tgttcctgcc gatacgatat cgattggtcc ccaccataag
    29761 taaccaatga tttggcaacc atcttttatt gcctcagcaa tttcctgaag atgtattttt
    29821 agatactcac aacgttttgg atcgtggatt cgattcaatt catctggtgc ttcatctagt
    29881 ccaatgccat tttcaactat gaataatggt aattcataac ggtctgtcaa ttcattcatg
    29941 acataacgaa accctagagg atcaattggc caactccaag gttcgggaga tttttccttc
    30001 aaaaagggat tatcgaaacc atttgctccg ccagtatcta cgcgcatttc tgcttctttt
    30061 ttatagacag cgctacgata ataactaaat gcaatataat ctgccgtgtt agcttgcaag
    30121 cactgttcgt ctccatctaa cattactggt gcacagtctt cttccttcca tgtcctgtaa
    30181 atataagctg gataatgccc tcggcacatg acatcggaga aaaaccatga attgcgttta
    30241 aattgcagtg aaccaaatac attctctgga tcacagtcat atgcataagt cgctaatgca
    30301 gaaaagctac acataatacc catcttagca tttggatcga ttttctttaa taattgagta
    30361 gcacgggcgt tcgctagaaa catatgatga caggcttgat aaatcacttt ttttgttagt
    30421 gtagcaatgg gttcaagttt atctctagca gggaatgtaa ccaatccagc agctgcataa
    30481 ggcatacgaa aagcattatt gatctcatta aaggtcatcc aatatttcac tttcccttgg
    30541 tagcgttcaa agacggtctg cgcataaaat tcaaaaaagt cgattaattt tcgattttgc
    30601 caccccccat actctcctac caatgctaag ggggtttcat aatgactaag agtaattact
    30661 ggctcgatat catatcgatt taatgtattg attagtctgt cataaaatac gagaccagcc
    30721 tcatttggct tctgttcgtc tcctcttgga aatattcgtg cccatgagat gcttgttcga
    30781 aaagctttta aacctaactc cttcagtaaa cgaatatcct cttcaaatcg atgataaaaa
    30841 tcaatcgctt catgagaaag ataatggagt gattcgtcta aagcaatctc ccaaatcttt
    30901 ttttcttcat tccactgaat actaggatgc tggttcatga tcccgataag cgtatctgtc
    30961 acattaggta atttgccgtc ttccaaccat gcaccttcac attgattagc agctactgct
    31021 ccgccccata aaaaattttc tggaaatatt tcctgatcca taactatctc tcctcgctat
    31081 caacaatttc ttacttgaag tatagctcga ttcatcaaaa caagatattc taactatttc
    31141 ggcagaagag aagaaaaaaa ttgaatgcta ctcttcattt ttaatattta tatataacaa
    31201 atacataaga cgatccccga aagaaaacac tcgttaacca tctaagcaaa cgatttattt
    31261 tacttccaac acaaatgctt cataaggtcg taaatcaact ttgccgtctg gtaactgttt
    31321 cagaggataa tttgacacaa taacccttaa agcttcacgt ggaatagtga acgtctgctt
    31381 ttcactggac atattacaaa taacgagcca ttgctggtct tcatatatgc gagaataggc
    31441 aaagacatgc tcaggcgtct tttccactaa ttcatagtct ccccagacga tgatgtcatg
    31501 ttttctacgc aaatcgatca atttcttgta catataaaaa acagaatctt gatctgctaa
    31561 cgcttcttct gcattgattt ctttataatt tggattgact cgaagccatg gaattccagt
    31621 agtaaaccca gcatgttcac gggcgttcca ttgcataggc gtcctagcat tgtctcttcc
    31681 tttcgccttg attgattcca agatttcctc ttttgtaaat ccagaagaaa tcctttcgtt
    31741 atacatattg attgtctcaa tatcttttgc ttccgcgata tcatcgattg ggcaattcgt
    31801 catgcctaac tcttctcctt gatagatata aggggtgcct ttcatcatat ggagcaagat
    31861 agcaaacatc ttggcacttt cttttcgata cacatcgtca tttccccaac gagaaacaat
    31921 tcgcggaaga tcgtgattat tccaaaataa actattccat ccttcatcac ctaaagatgt
    31981 ttgccatttg gataatactt ttttcagttc agagatactc aagggtttca aatcccattt
    32041 gtctttgcct tcttgctgat ctaatcctat gtgttcgaat tggaagacca tggataattc
    32101 atttctttta ggattggaat aaagtttagc gatttcggta gttgctcccc atgtttcgcc
    32161 aacagtcatc agatttttgt caccaaaagt tgcttgattc atttcctgaa gatactcatg
    32221 cagtttaggt ccattacttg tgatttcttt atccggttct ttgccaatca tatcgatcac
    32281 gtccatacga aagcccccaa tacctttatc gatccagaaa ttcatcattt gataaatctc
    32341 ttggcgaacc tgtttatttt tccaattcaa atcaggttga cgcttgctat acaagtgcag
    32401 aaaatattgc cccgtttttt catccaactg ccaagcagaa ccactgaata ttgagcgcaa
    32461 tctattaggc tcttttccat tgaccggatc acgccaaata taatagtccc gataaggatt
    32521 ttctttattt ttacgggcct caatgaacca cggatgttca tcagaagtat gattcaccac
    32581 cagatccatg atgatacgga tatttctttt ctttgcttct tcaatcagtt cttccatttc
    32641 ttccatcgta ccgaattctg ccatgattgc ttgataatta ctgatatcat acccattgtc
    32701 atcattcgga gactcgtata ctggagaaag ccaaattgca tcaataccta aatacgccag
    32761 ataatccaac ctggaaataa tcccccgcaa atctccaata ccatcttgat ttgaatcttg
    32821 aaaacttctt ggatagattt gataaacaac tgaatggtgc caccattttt gttccatgat
    32881 tttcctccta taaatggatg tttctatttt ttgcttaatc atttatttta gtctctctca
    32941 ctaaaaattg taacgctttc aaatagctat tgcaccttgt tatcggtaac agaataaaac
    33001 aaaaaaacag ctgaggaaat tccctcaact gttttttgaa aggattgatt tatgcaggga
    33061 agaatgctga tttcacaact tctttataaa cggtgttcac tcagctgtcc cttggtatta
    33121 gctcaaaaaa cggcaaaata taagaaacta ttttttcgtt tttttctcta atactcaggc
    33181 cagaacgctg atttcacaac ctctttattt cacattcaat atccacatga atcccacaaa
    33241 tacaaagaac agaccataca tgattggaga tacttctttt cctcttttag ctgctaacat
    33301 ggtaattgga tagaagataa aacctaatgc aatcccatct gaaatactat aagtcaaagg
    33361 catgccgatc aagatcaaga atgaagggat cgcaatctct aagtttcccc attcgatttg
    33421 tcgcaaagat tgtgccatca atactcctac cataatcaat gctggtgcag ttacttgtga
    33481 agtaacaact gatagtaatg gagaaaagaa caagcctaac aagaagaaaa tccctgtagt
    33541 aatagctgtg atacctgaac gtcctccaac ggcgatacca gctgatgatt ctacaaatgc
    33601 accaacagga gaagtaccaa ataaggaacc cgcaagcata gctgttgaat cagaagctaa
    33661 agctttccct acacgaggca tcttgttgtc tttcatgaaa cctgcttggt ttgctaatcc
    33721 aactaaagtt cctgctgtat caaagaaagt cacaagcaag aatgtcagca ctacgaccca
    33781 catttgaaga gaattgatgt cccctacatg ttttaatgca actaaaaatg ttggtttcaa
    33841 acttggtgct gcggcaacaa tttcagaagg cattggaatc aaacctgtga ccagacctaa
    33901 aatcgttgta gctgccatcc cgatgaagat gccacctggt acacgccgaa caagcaagat
    33961 agcagttacg actaatccaa aaatcgtcaa ccaagtagaa ccaacgttga gtgatcctaa
    34021 tcctactaat gtagattcat tcgaaacgat gatcccacct tcgctcagac caaggaaagc
    34081 gataaataga ccgataccgc cagaaatcgc atatttcaaa tctgctggga tcgcatcaat
    34141 gatcatttcc cgcagcttga agattgtgat caaaataaaa atcaaagaag caacgaatac
    34201 gccggctaat gctgtttccc atggaatccc cattcctaaa caaacagaat aagcaaaaaa
    34261 agcattgatc ccaagagcag gtgccgttcc aattggataa cgggctaata cccccattaa
    34321 aacacagccg atcgcactgg ctaatgcagt cgcagtaaac actgctcctt catccattcc
    34381 agaagcacct aatacagttg gatttacaaa taagatataa gccattgaga taaatgttgt
    34441 aaatcctgcc agtacctcac gttttaccgt ggtgttcaat ttttctattt cgaagtagga
    34501 aagtattttt tccttcatct tccatccccc taaatccgaa cattataaat aaagtccgtt
    34561 aaaatttcga tttattaata aaacaacgtg ttttatcata tactaaaaca aaacataagt
    34621 aaacaaaaaa ttcggatttt aagcaaaatc cgaacgttcc atctgattaa atccctttat
    34681 ctcaaagttc atgaacattc actttggatt acaagaattt ctttattgtg ttaataaagt
    34741 ttcttcattc tagtctcaaa aatcaatagt gtgatttgat tttaccattt cgtttctgtt
    34801 cttctttgat gaattctttt gtatcgacag gattcagatt atttagtccg ccaaaacctg
    34861 tatcgaccat ttttccccag ccttttgtct tcttttttcc tgatttgaga ttctttgctc
    34921 gaagcttctc actatgtttt gctcgccgat acttcaaata accttctaat cttcccatta
    34981 gatccactgt ttttcctcct ttttattcaa tcagacgact cagcttttcc tataacaaaa
    35041 taattgcctt cagggtcaga aaaattgaaa gtcaagatgc cattgttatc tgtgatttct
    35101 cctggttctt ccatgttctc gtagatagtt tcaaattcat ccgtaaaaaa tactaaagaa
    35161 ggcggtggtc ctaatacctc tggtgaaaac ttctgcacaa atacttttgg gaatacgcct
    35221 aattctaatt cttttgagat gctgagtaca atactcttaa attcttctgg caattcgatt
    35281 gtttcagaaa tttctgcacc aattttttct acaaagaatc ggcaaatcat ctctacgtca
    35341 ttgacataaa gcatcacacg tgttttttgt atcatcattt gacccttctt tctatttgat
    35401 tttctttcct gtctataaac tgattccatt atatcacgaa agcgcaaaca ccttgtgttt
    35461 gatactattc aagaaaaata aagcaatagt ttctaggatt gctttcctcc acaaactttt
    35521 tttacaaaaa aaaaacggca atgaatgccg ctcttcttat ttcgattctt ttataattct
    35581 gcttctgatt catctttggt tagtgtgtgt ctaacactag ttacgccaaa tgtttgatcg
    35641 attttttcct tcaacgcttc gattgcctgt gcgccacgtg ccgcttgttc agcattgatg
    35701 gattcaatga ccagcaccgc tgcttgtgtt tcttccgtca gcatcgttct ttgggcttca
    35761 cgccagttga gacagcgaca gatcgctcct tgttcatcat agtagatgat ttcttccgga
    35821 agtgccggag catcggaatc tgcacctaaa ggcaggaatg attcgccacc ttgggctttt
    35881 cctaaatgca gatttcctgc gatcttttcg atatcttctc cgccgcaagg tacgccaaaa
    35941 gacaaggaaa tactattata gatatctaca agcggattga ttgggaaaaa ttcccgttct
    36001 tgatttgcac gtttcagtaa agcttcaata gaagaacgtg cacctttttt agtcttgaat
    36061 gtggtaaatg cctgacgcca ctgagcaatg acttcgtttt ggctgaatgt ttcttctgta
    36121 aggtaatttc ttgcttcttt tgctgcctca ttcaataatt gatgatacgt ttcttcactt
    36181 tcttttgtta cgtggttatc gataccattt acggataaga tttcgatggt tgcttcagga
    36241 aatacttccc agaaattggt atccactatt acttttttca tctgttcaca cctcttgatt
    36301 agaataattg cttgatgaat agatagtctg tttggggatc atctcccata taaaaaacat
    36361 gttcgctgta tttgataaat ccttttttac gataaaaagc gagcgcaaca tcgttgtgtt
    36421 cccaaacacc gagccaaatg gctgtttttt gcttttcttt tgctttttca atagctgtat
    36481 ctagtaaaaa tgtaccgata ccttgatgtt taaatttttt tcgtacatag attcgctcga
    36541 tttctaaaaa atgatcagcc atttcctcag attgactcgt tcctgtattt agtttcaggt
    36601 aacccgcagg ctcttgatcg atactcacta ggaaaaaatc ggtttcttta tcttttagct
    36661 cttctgtaag tttttctagt gcataagctt gtgtcaagta atcagctaaa tgctccggtg
    36721 aattatcttt tccaaatgta tctgtaaatg tttcgatact aatttgttgt agtaagtcta
    36781 catctgaagg cgtcattttt cgaatttcgt atttattcac ggcttcttcc tttcaataga
    36841 tccttttatt gccttttttg acaaaattcc aatcttcgct aacattcttg catactttat
    36901 ttaaaagata ttccaattgt tttgcttctt cgtcagaaag accatttaat gcgactgcat
    36961 tggagtagtt gttttctcga atgatgaaag gatagatttc tgctcctttt ttagtcggat
    37021 agagtttctt gattttttta ttttctttat cttccaaacg ttcgatcatc ccattggatt
    37081 ctaatttctt gattgctcgg gcagtcgttg tacgatctac tttgatcatt tctgctagtt
    37141 tttcttggat gattccagga ttttcgcaaa tacgtactaa ataaagatac tgtcctcttg
    37201 ttaaatcgac ttctttgaat tcgatattgc taatagaatc tagcgctcgg gcaatcattc
    37261 cgatgtctcg aaggatttca gccatttcat cccaccttta ttttgtttgt aagactagtt
    37321 cacctctttt tgttgcaaat gcaactattt tcaataaaaa aatcaagttc ttttttaata
    37381 actagtaaag tatattcttt tcttctttta cgaaaaagct caatcacaat agatctggtt
    37441 tttgttttct aaaataagta actgtttctt cgttgttcgc ttttctgata gctttggcaa
    37501 ctcttctata ctaaaaaatc ccatgttgga agtttcatta ttctcaataa acttggcctc
    37561 ttcaccgtgg atcgcacaag caaaaacaag tttataatac tgaaaaagct gtggaatatc
    37621 tttttgttta ttcgtatcaa aaactgctcg caactgagta gtttctactg ttaatcctgt
    37681 ttcttcatag acttctttac gcacattttc ttcaggagac aatcctattt cagcaaatcc
    37741 accaggaagt gaccattctt tcgtccgttt atcttcgatt agtaggacct tgccatcttt
    37801 tttgatatag gcacgaacat ctacttttgg tgttggatgt ccttctccaa tcgttgtgag
    37861 cgcttttatt tcttcgaaag tttcttttcc tatttcactg atcagttcca atgaaatcgt
    37921 ccggatctcc tcaaaacgct ctttatcaaa tacatctttt ccataatata gaccagcttg
    37981 agaaagtgcg agtagtcttt tgtattgagc caacttcttg ttcattctct tcccttcttt
    38041 tttagcaaat gtatgactat gtaatcaagt atagaagact ggtttaatta cggcaaataa
    38101 atataatgaa atctgcaata aataaaagaa tgttaaaaaa ctcgagaaca aaagacattc
    38161 gacttctctg gggctgtgaa taaatggcgt tcaaaagctt gcatctgcgg taataagccc
    38221 caccaaacga aaaatatgac gagctattct tgtctggctg ttcttattaa ttgaggcagg
    38281 acagcttttt cacaacctcc ccactaaacg gtgttctcct accaactcct cggtcacaag
    38341 tcacaatatt catcaaacat gagaagctgt ttttttgaaa ttgctccttg tgactcggag
    38401 ctgaacgaca ggctcacgac ctctcggtaa acagcgttca aaagccaatt tttcgatatt
    38461 aagccatttc tttgttactt attgtcattg atttcttttt ctaatttatc caatagtgga
    38521 gtgtaatcca cttgagtata gtctgttaca tcaaattcat atagtgttcc taactggaat
    38581 ttgtcatatt ttcggtcggc gatgatcttt ttgaatgctt ctttcgtaat atgattggtc
    38641 cctttcgttc gatcttccaa tacgtcacca tagtgataat gatccatgat atagctcaaa
    38701 tgacgatctg attcacgatc acgtttgtac cgacgttgcc ataacacttc aaagtctgct
    38761 gttaggcgaa tcgtgatcac ctgatatccg tatctatctg ctaattcttg gagtcgtgct
    38821 ttttgcttat cactaaaagg atactcggac acaatcactt tctttcctgc ttccatatac
    38881 aaatcaagtg ctttgtaata gaaaggataa actttcgtcc tttcttgttc cgcccgttct
    38941 tccaaattgt taaacccgat ggattctgca tagtattcct tgatttcatc gagcgtgatg
    39001 gcatacatat ctggaaatcg ttcccgtagt ttattcataa gatatgtttt tcctgtacca
    39061 ggactaccag ccagaagaat gaaatacttt ttcatctcat ttatttccta agttaattgg
    39121 actttccaag ttcataaaca gtccaatctt atgcttgaca atttctttta ctgcttcatt
    39181 tgctggaatc acattatggc gaagagactt gttgatttct gtttcactaa aggtttcttt
    39241 agctactttc gtccagttga ctaaaagctc cgttccaaca ttgaatttgc ggatcccgtt
    39301 attgatcaat tcaggataat cttcttcttt cacacctgtt cctccatgga taactagggg
    39361 gacatcaact actgcatcga tttctttcag caagggaatg tttacttctg tttttgattt
    39421 gaattgtcca tgattcgtac cgatagcaat agctaatgca tccacgcctg tagcttctac
    39481 aaatttcact gcgtcttccg gtcttgtata taccttgtca ttttcagcga catggattcc
    39541 ttcttctgta ccgccgattg ttccaagttc tccttctacg gaaacaccgt gcgcatgggc
    39601 atattctact actgcttttg tcttcaggat attttctttg aaaggcagat gggacccatc
    39661 atacatgaca gaagtatatc cgctgtcgat tgcttctttg atatcatcaa agtctcgggc
    39721 gtggtctaag tgcaagactg catcaaccat ttcttcttct gcaatagctg tcattacatt
    39781 gactaacaca tgcatgccga tatatttagc ggtatcgaca cttgtttgaa taatgatcgg
    39841 cgaacccatt tcttttgctg cgcctagcat agcaggaagc atttctaggt tatgcgtatt
    39901 aaaagctcca actgtaaaat tcaactcttc tgctgttttt gttacttctt tcaatgttgt
    39961 atacatcttt tattgccacc ttatcttttt atttgtttcc tttgatcgtt acttgttttg
    40021 caatttgtcg catctcatcc attttgccca tgtagtaatc gatcccttga tcatcaccag
    40081 cacgagcagc atctgcacgc ttctggttat agaggctcat cagtttttta tacccatctc
    40141 cgatcgtctg tttattttcc aaactctttt ctaaatattg gatggcttga tcgttcacat
    40201 acaattcaga aaatttaagt ccaacttgtt cataaagttt cgctaattta tgcttatctt
    40261 gggtagtgga aatctcttgt ttcaattgtg taatctctga ttgaatcttt tcggtcgctt
    40321 ctggcgatag ggctttttct tcttttgcca gaactttttt cggttctttt tcttttttct
    40381 taaaaaatcc aaacatcatt ttccctactt tcaattaaaa tgctttcaaa actgggctta
    40441 agatccaggc atataagagt gctgcggcta ctgtatctac atcggttgct gtagcattgt
    40501 tgaatcccat cgaattaaag atcgttacta gcaaagcggg caataatgta ataaagaaac
    40561 catgagcaat accgccaatg atcgctcctc ggcgtccgcc aactgcgttg ccaaatatcc
    40621 ctgctgtccc tccggcaaag aaattggtta gcataccagg taaaatcatc gctaagccga
    40681 acatgggcaa agtaaacatg gcaataatcg taccaatcgt tgtcgtaatg aaacctaaaa
    40741 tgacagcatt tgggctataa ggaaagaaaa ctggacaatc taaggcaggg atcgcatcag
    40801 gaactaattt catagctatc cctcgaaatg ccggcacgat ttcacctaac agtagacgta
    40861 caccagctag caatacatac acacccacta cgaattggat ggcttgtaaa aaggcaaaca
    40921 tgatatagtt ttgatctcct gataattcag aagcgaatgg ttcgcctgca aataaaacag
    40981 tgatgatata tagcgggacc atcacgacca tgacagacaa ataagtatct tgcagaaatt
    41041 cgaaatgtgt cggcaacttg atatcttcga tcgacttttt attttcgcct ttctcaccaa
    41101 aaagcttagc aacaccggct tcaaataagt acccgatcgt acaaaaatga cctaaagcaa
    41161 tatcatttga tccagtgatt ttacgaatga tcggttgcgc tactgccggc atagcaatgg
    41221 cgaaacaagc tccgataaaa ccaccgacca agatcaatac gatccctcgc agaccggcaa
    41281 aatagccaaa gactgtcgtc atcgttgcca tccataaaat tgcttgacca gttaaaaaaa
    41341 tgtacttcca ttttgtgaaa cgagccaaaa taatattgaa tacaaaaatg gctaagaacg
    41401 tcagtgcaat atctctacct aaacccaaat cattcattgc ttgaccattg atagattcaa
    41461 tagaagggat gatcccttgc atatgaaatc cttcagtaaa gattttcccg aaataagtta
    41521 aacttccaac gatgatgctc gaaccggcac tcaacacttg aaatcctagg agtgtcttta
    41581 acgtgccaga gatgacttgt cctgctgatt tcttctgcag tagtagaccc aacatagcaa
    41641 tcaatgcaat ggtgatcgat gcctgagtca aaatattttc gatgataaaa ttgataacgc
    41701 ccatgttttg atgtgcaccc caaaagttag actagaaatc taattaaaag gggtgctttt
    41761 tttgcggaaa tatacatttg tttttaagaa aaaagtagtt tcagactatt taaataacga
    41821 aggcggctac aaatatcttg cacataaata tcaaataaat cgtacactgg ttagacattg
    41881 ggtaaggatt tataactatc atggttggga aggcttggtt ggaggtggca aaagctacac
    41941 tacaaaattt aaacttgatg ttatagaata tatggaaaca aatggtcttt ctatccaaga
    42001 aactgctaaa aaatttaata tcggttcaaa tagaactcta agtaaatgga tagagcaata
    42061 tgaagaaggc ggtgcttctt cacttgagag ccaaaaaagg ggcagaaaaa ttagtatgaa
    42121 ttccaagcta aacattccta aaaaacttaa agatgagtct cttgaagaag aagttattcg
    42181 tttaagagca gagaacgcat atttaaaaaa gttagaaacc ttgattcaag aacaagattt
    42241 gagcaagaag aaatcaaggt taaaacaatc cttcagctca aaaaagaatt taaattgaat
    42301 ctattacttt caattgcaca attagcgaag tcaacttatt attactgggt aaaaaaatta
    42361 gataagccag ataaatatag caagataaag caagaaatta cagcgattgt aaaagaatca
    42421 agaaactctt atggttatcg tagagtcact ttagcgttaa agatgaaagg atatacaatc
    42481 aatcataaaa cagttagaaa attaatgtcc caaatgggac ttacctgtca aatccgaata
    42541 aagcgatata aatcttataa aggaacagta ggaaaaattg ccaagaatgt gttaaaacga
    42601 aatttttcag tagatacacc caataaaaaa tgggtgacag acgtcactga attcaagata
    42661 aaaggaagaa aaatctatct atctcctata cttgatttat ttaacggaga aataattagt
    42721 tatagtatat caactagtcc aacatataaa ctgattgaag aaatgcttca acaagcaatt
    42781 aaaaaaaagg gaactgaggg ttcactgatt ctacattcag atcaaggatg gcagtatcag
    42841 atgccacaat atcaaaaaaa attaaaagag aataacatta ttcaaagcat gtctagaaaa
    42901 ggaaattgct tagataattc tgtaatagaa aatttttttg gtgtgcttaa atcagagttt
    42961 ttttatcgag aaaaattccg atcaattgag atatttcaaa gtaaattaaa tgagtatatt
    43021 aggtggtata ataacaaaag gataaaacta aagttaaatg gactatctcc agtagaatat
    43081 cggaaacagt ccattaaata gttcaacttt tggggttcag ttc
//
LOCUS       DADVXU010000013        34960 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.014, whole genome shotgun sequence.
ACCESSION   DADVXU010000013 DADVXU010000000
VERSION     DADVXU010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 34960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.014"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="KB953_000700"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="KB953_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HBB6865697.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="KB953_000701"
     CDS             complement(2020..2922)
                     /locus_tag="KB953_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBB6865698.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3611)
                     /locus_tag="KB953_000702"
     CDS             complement(2919..3611)
                     /locus_tag="KB953_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HBB6865699.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="KB953_000703"
     CDS             complement(3654..4295)
                     /locus_tag="KB953_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBB6865700.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4333..4647)
                     /locus_tag="KB953_000704"
     CDS             complement(4333..4647)
                     /locus_tag="KB953_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865701.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4635..5987)
                     /locus_tag="KB953_000705"
                     /pseudo
     CDS             complement(4635..5987)
                     /locus_tag="KB953_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372436.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
     gene            complement(5984..6916)
                     /locus_tag="KB953_000706"
     CDS             complement(5984..6916)
                     /locus_tag="KB953_000706"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBB6865702.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6913..7689)
                     /locus_tag="KB953_000707"
     CDS             complement(6913..7689)
                     /locus_tag="KB953_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HBB6865703.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7743..10928)
                     /gene="carB"
                     /locus_tag="KB953_000708"
     CDS             complement(7743..10928)
                     /gene="carB"
                     /locus_tag="KB953_000708"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBB6865704.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10933..12009)
                     /gene="carA"
                     /locus_tag="KB953_000709"
     CDS             complement(10933..12009)
                     /gene="carA"
                     /locus_tag="KB953_000709"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBB6865705.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12014..13294)
                     /locus_tag="KB953_000710"
     CDS             complement(12014..13294)
                     /locus_tag="KB953_000710"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBB6865706.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13313..14239)
                     /locus_tag="KB953_000711"
     CDS             complement(13313..14239)
                     /locus_tag="KB953_000711"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HBB6865707.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14252..15535)
                     /locus_tag="KB953_000712"
     CDS             complement(14252..15535)
                     /locus_tag="KB953_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBB6865708.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="KB953_000713"
     CDS             complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="KB953_000713"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HBB6865709.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16323..17234)
                     /locus_tag="KB953_000714"
     CDS             complement(16323..17234)
                     /locus_tag="KB953_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBB6865710.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17231..17695)
                     /locus_tag="KB953_000715"
     CDS             complement(17231..17695)
                     /locus_tag="KB953_000715"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBB6865711.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17700..18398)
                     /locus_tag="KB953_000716"
     CDS             complement(17700..18398)
                     /locus_tag="KB953_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBB6865712.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18385..18771)
                     /locus_tag="KB953_000717"
     CDS             complement(18385..18771)
                     /locus_tag="KB953_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865713.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18918..20588)
                     /locus_tag="KB953_000718"
     CDS             complement(18918..20588)
                     /locus_tag="KB953_000718"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HBB6865714.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20771..21004
                     /locus_tag="KB953_000719"
     CDS             20771..21004
                     /locus_tag="KB953_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HBB6865715.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21103..21534)
                     /locus_tag="KB953_000720"
     CDS             complement(21103..21534)
                     /locus_tag="KB953_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HBB6865716.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21613..22026
                     /locus_tag="KB953_000721"
     CDS             21613..22026
                     /locus_tag="KB953_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HBB6865717.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22064..23212)
                     /locus_tag="KB953_000722"
     CDS             complement(22064..23212)
                     /locus_tag="KB953_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBB6865718.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23334..23930)
                     /locus_tag="KB953_000723"
     CDS             complement(23334..23930)
                     /locus_tag="KB953_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBB6865719.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="KB953_000724"
     CDS             complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="KB953_000724"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBB6865720.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27160..27591
                     /locus_tag="KB953_000725"
     CDS             27160..27591
                     /locus_tag="KB953_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB6865721.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27636..28985)
                     /locus_tag="KB953_000726"
     CDS             complement(27636..28985)
                     /locus_tag="KB953_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBB6865722.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29055..30218)
                     /locus_tag="KB953_000727"
     CDS             complement(29055..30218)
                     /locus_tag="KB953_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBB6865723.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30347..30670
                     /locus_tag="KB953_000728"
     CDS             30347..30670
                     /locus_tag="KB953_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865724.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30853..32202)
                     /locus_tag="KB953_000729"
     CDS             complement(30853..32202)
                     /locus_tag="KB953_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBB6865725.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32271..33224)
                     /locus_tag="KB953_000730"
     CDS             complement(32271..33224)
                     /locus_tag="KB953_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBB6865726.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33326..34516)
                     /locus_tag="KB953_000731"
     CDS             complement(33326..34516)
                     /locus_tag="KB953_000731"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HBB6865727.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         9951 a         7641 c         6120 g        11248 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagattatt ctcttctttc tgctaaccag tcttagaaaa atgaatttta aaaaaatcaa
     4501 aaactgatca cgatagaagt ttcttttcat acctgatgaa aagatgcttc tcgcaatcag
     4561 tttttggatt tttgctattc caattgatga ttctggagac acaaatcgga tttctctatc
     4621 ttcatcaagc gcgcttaaat ctgccaattt tcaggatctt ttttccattc ttttagcaga
     4681 gttaattctt gctcatcgat ataacgatct tctaaagctg cttcaatcaa tacagaataa
     4741 ttggtcaacg tcataagagg gatttcggct ttttcgaaat ttgcttttcc ttttggcaac
     4801 tcatacgtaa agatagccgc aacaccaagg atatccgcac cttctctttt tgctgcttca
     4861 gctgcttcca agacacttcc gcctgtagaa atcaaatcct cgatcacaac catcttctga
     4921 ccttcaaata tacgtccttc gatttggtta cctttcccat gatctttcgc cttacttcga
     4981 atatagacca ttggcaaatc cagtatttcc gctacccatg cggcatgagg gattcctgct
     5041 gttgcagttc cagcaatcac ttgtacttca gggtaagctt ctttgatttt gcttgctaat
     5101 ccttttgcga tctcttttcg gacttttgga tagctcatcg tgattcggtt atcacagtaa
     5161 attgggcttt tgattccact tgcccatgta aaaggattac ttggatttaa aaatactgct
     5221 tcaatttcta ataagtcttt tgcgatactt tgttcaacat tcattttagg ttagctccat
     5281 tctgtttgaa tcgtttgata tgctttataa ggattagttg actgagtgat tggtcgacct
     5341 ataacgataa aagttgagcc gatctttttt gcctgagaag gtgtgactac tcttgtctga
     5401 tctcctgcga ctgttccttc tggtcttata cccggagtta agcaaacaaa ttcgtctcct
     5461 gccatttctt tgatcgcttc tacttcccaa gcagaagaaa ccacaccatc taatcctgct
     5521 tttttggcac agctagcata atggatcaca cttttttcta aagaaacttc gattagttga
     5581 tcggcatgca tttcttcttc acttgtagaa gtaagctgag tgatagctag aagtagcgga
     5641 cgttttccac cttctggagt cccctcttct aatcctcgca ttgccgcctc catcatacga
     5701 ataccgccag cggcatgaac acaggtgaga tcaacaccaa gctttgccaa tccccgcatt
     5761 gctttttcga ccgtattcgg aatatcgtga agtttcagat ctaaaaatac attatgacca
     5821 gcctctttta gataacgtac gatttcaggt ccttcttggt agaacaattc cattccgatt
     5881 ttcacaaaca atttttcttc tttcgggaaa aatcgaagaa agtcttctac ctcttgccta
     5941 gttgaaaaat ctaatgcaat aattggtcgt tggtctagtt gattcatcgt tcacgttcct
     6001 ctctcacttc tttgattaat ctttccagcg attcgatgcc tagttcgctc attcgcacag
     6061 ggagctcttt aattagttta ggacagatat acggatcggt gaaattcgcg gtccctatag
     6121 ctacagcact tgctccagcc ataaacatct caagaacatc atccactgtc tgcacgccgc
     6181 ccatcccaat gattggaagg ctggatatac ttgctacttg gtgaatcaaa cgaatagcga
     6241 caggcttgat tgctggtccg gagagtccac ctgtttgatt ggctaaaatc ggttttcttg
     6301 tcttaaggtc aatccgcata ccaagaagag tattgatcat cgtaaaccca tctgcccctc
     6361 cagcttcgat tgcctgagcg attggaacga tgtctgttac atttggggac aatttgacat
     6421 agacaggaac agaagaaact ttctttactg cttgtgttaa ttgaaaagca acatcagaat
     6481 ctgttccaaa agcaattccg ccatgtttga catttgggca agaaatgttc agctcgattg
     6541 ctttcacatt tggtgcttcg ccaattttgc tacaaacttc tacatagtct tcttcacaag
     6601 caccagcaac atttgcgatg atgggtaact cattgtactc ctctaattct ggaagaagtt
     6661 tatgcataac aacgtctaga ccaggatttt gcaaaccaat tgcattcaac atgccagatg
     6721 gtgtttccgc cactcttggc gtgggattgc caaagcgggc atttggagtc gttgccttga
     6781 ccatgatcga tccaagctga ttcaaatcat agtattttcc gtattctttg ccaaaaccga
     6841 aacagccgct tgccggcatg ataggatttt ttaattctag gccaggaagt ttgaccgcta
     6901 aaggattctc attcatataa ttacctctcc tattttaaaa atcggtcctt cgtcacaaac
     6961 tttcacactt tttgttcctg tttcatcttc cggcgtatga caaacgcaag catagcaagc
     7021 ccctatccca catgccatgc gggattctaa tgataactgg acattttgat gcgaagaaaa
     7081 cagctgttcc actgtcttca ataacccgtt tgaaccacat gcaaaaacag ctgtaggttc
     7141 cgtcttcgtt cctaacaata aattaccgac atttccgtgc ataccgtatg ttccatcgtc
     7201 agtagcgatt cttgtttctc ctagacgatt aaattctttt tcataataaa cgacttcatg
     7261 tgtggcaaaa cctaaaaaat gcgttggctt aattcctttt tcgactaact gttttgataa
     7321 ctcatataga ggagggacac cgattcctcc accgattata aatacttcgt ctcctgtatg
     7381 aagattggac aaatcaaaac cattgcctaa aggacccatg acatctaaat ggtctccggc
     7441 agaaagagta gagaagactc tagtcccctc accttccacc cgataaatga tacggcaagt
     7501 atttgtttcg tggtcgatat gattcaagct aatcggtcgg cgtaatagca agtctgcacg
     7561 cggcacgaga atatgcaaga actgtcctgg ttgctgcatt tcttttacta gatcaccttt
     7621 caatgtcatt tcaaaaatct ttggtgccag ctgtttttgc tgaacgattt ccatcatttc
     7681 ttgtttcatc tgtcccctcc tgcaacggtt tttcgtcttt ttgtcttaac atgtgctcgc
     7741 cgttaaatag ggttagttga aaaagcacgg gattccatca ctcgaacgat cgcatctgct
     7801 gtgtctaaag aagtaaacaa tggaatacca tgttcaactg cttctcttcg gatgatgaaa
     7861 ccgtctttag aagcatttcc ccgatcttta tcaatcgtat tgacaaccac ttgtgtttta
     7921 ccatctctta ttaagtcgac tacatttttt tctgctgctt cctcagaaat tttcgctact
     7981 gtttcaacga ccagaccatt tttttcgaaa taagcagctg tatgtttcgt tcctatcaga
     8041 ctatagccaa tttctgtaaa acgtttagct aattctagtg cttcttcttt tgtttcgtcc
     8101 gcaatcgtaa ataatacggc tccatattca ggcaagtgta atccagaagc ttcgaatgcc
     8161 ttgtacaaag ctttgtctaa ttgtctgtca gatcccatta cttctccagt cgatttcatt
     8221 tcaggcccta agtaagtatc aactttctgc aatttcgtaa aagaaaagac aggtgcttta
     8281 acatgaactt cttttgtttc tggataaagt ccgttttcat aaccaagtgt ctttagtttc
     8341 tctcccaaaa tagctttcgt cgcaatttgt gccattggaa ttcctgtcac cttacttaag
     8401 aatggaacag tccgacttgc tcgaggattg acttcgatca cataaacctc attttcatgg
     8461 atgacaaact ggatattcat catcccgaca caattcaaac ctaatgctaa gcggatcgtg
     8521 taatcttcga tcgttttctt gatcgattca gataatgttt gaggtggata cacagccatt
     8581 gaatcccctg aatggacacc agcacgctca atatgctcca tgatccctgg gatgagtact
     8641 gtttcaccgt cacaaatcgc atccacttcg cattcaccac caagcaagta acggtccact
     8701 agtactggat gttctggaga tgctttcaca gcatgttcca tgtaatcttc taaatctcgc
     8761 tggttttcaa cgatttccat cgcacgtccg cctaatacat agcttggacg aacaagaact
     8821 ggataaccaa ttctcgaagc gataaccacg gcttcttctt tacttgtagc agtatcccct
     8881 aaaggttgag gaatatccaa ctctttcagc gcttgctcga ataaatcacg attttccgca
     8941 cgatccaagt cttcgatcgt tgtccctaag atttttacac cagcagcagt caaaggttcg
     9001 gctaagttga ttgctgtctg accaccaaat tgaacgatta ctcctatcgg ctgttctagc
     9061 tcgatcacat tcatcacatc ttcgaaggtc aatggctcaa aatataattt atccgaaata
     9121 gaaaagtcag tcgaaactgt ttcaggattg ctgttcatga tgattgcttc gtatcctgcc
     9181 tgctggattg ctttgacaga atgcacagtc gcatagtcaa attcaacccc ttgtccgata
     9241 cggataggac cagatcccag tactagaatt gagggctttt ctgatcgtga actttcattt
     9301 tcatattcat aggaactata aaaataaggt gtttttgatt cgaattccgc tgcacaagtg
     9361 tcaaccatct tatatacagg aatcaaatca ttttcttttc tcatctggcg aacggcttct
     9421 tcgtttattt cccacagctc cgccactttt ttgtctgtaa aaccgttttg tttcgctgtt
     9481 ttcaatatct tgtcatccaa cggctgctgt ttcaactgct gttcgatttc tacgatatgc
     9541 aacagtttat ctaaaaagaa taaatcgatt tttgtcaaat ccgctaattc ttctatggtg
     9601 taccctcttc taatagcttc agctacataa aacaggcgat catcttgtgc tcgaaccact
     9661 ttttggatta ggtcatcgtc cgtgatgtct gccaactcag gaagttcgat atggtgtgta
     9721 ccgatttcta aagaacgcac agctttcaat aaagactctt cgatattccg accgatagcc
     9781 atcacttcac cagttgcttt catctgcgtg cctaaaagcc gttctccgtt ttcaaactta
     9841 tcaaacggcc agcgaggaat tttagctaca acataatcta atgcaggttc aaattctgca
     9901 aaagtcgttc ctgtaacagg atttttcatt tcgtctaatg ttaatccaag cgctatttta
     9961 gcagctagct tagcgatcgg atagccagtc gccttacttg ctaacgcaga tgaacgagaa
    10021 acacgcggat tgacttcaat cacataataa ttaaaactat gtggatcaag agcgagttgg
    10081 acattacagc ctccctcgat cttaagtgct cgaataatcg aaagagaagc atcacgaagc
    10141 atttgatatt catggtcaga caatgtttga ctaggggcaa atacgataga atcacctgta
    10201 tggataccca ccgggtcaaa attttccata ttgcagacaa cgattgcatt atcagccgaa
    10261 tctctcatca cttcatattc aatttcttta aaacctgcaa tacttcgttc aatcaaacat
    10321 tgggtagcgg gggaaagact taatccattt tctgatacga tacgtaattc ttcttcattt
    10381 tcacacatac ctccaccagt tccgcctaaa gtaaaggctg gacgaacgat gacaggatag
    10441 ccgattttat ttgcaaaatc tacagcttct tctactgtat tgacgatttc agactcgggg
    10501 attggctgat tcaattcttt catcaactgt ttgaatagat cacgatcttc tgcttgatcg
    10561 atcgcagaaa gctttgttcc tagaagttct atgttcaatt cttctaaaat acctgatgct
    10621 gccagctcca ttgccatatt gaggcctgtc tgtccgccta aagttggaag gagagcatct
    10681 ggacgctctt ttcgaagaat ccttgataca aattcaagtg tgatcggttc gatatatact
    10741 tgatctgcca cttctttatc cgtcatgatc gtcgcaggat tagaatttac taaaaccact
    10801 tcatatcctt cttcttttaa ggcaaggcag gcttgtgttc ctgcgtagtc aaattctgcg
    10861 gcttgaccaa tgatgatcgg accagatcca atcaccataa ttttcttgat atctgtacgt
    10921 ttcggcatct gtttactgct cctttcctgc gtccatcatt tccataaatt catcaaataa
    10981 atgtagtgca tcatgcggac caggggcagc atctggatga aactgcacac tgaaagcggg
    11041 ataatctctg tgtttgactc cttctactgt tccgtcattt acttctacat gagttacgat
    11101 gagtttttct ggatcaatcg tggcttcatc gactgcatag ccatgatttt gagaagtaaa
    11161 atcaattctt ccggtcgcta cttctcgtac cgggtgattt aatcctcgat gtccgaattt
    11221 cattttatag gtatctgcac cgttggctaa agcaaataac tggtgaccga gacaaatacc
    11281 aaaaatcggt acttttcctt ggattttttt gatcatttcg attgcttctg gcacatcttt
    11341 tggatcacca ggtccattcg tcagcatgac tccgtctggt gatagcgcta aaatcgtctc
    11401 ggcatctgtg ttatagggaa gtactgtgag gttacagttt cttttactca attctcgtaa
    11461 aatactatgt ttcaagccga agtcaatgac aatgactttc cgacctgttc ctggacttgg
    11521 ataaggtttg ttagttgaga cttgctgcac ttgattggtt gctagaacgg ttgctttcag
    11581 ctgatcaaat gcatgttcga aggaatctcc agcatcaact atactagctt tcatcgttcc
    11641 gacttcacgg attttgcgag taagggcgcg cgtatcgata cctgatattc caggaattcc
    11701 tttttgtttc aaaaatgaat ctagcgtcat ttggtttcgc cagtttgaag ctacacgtgc
    11761 atgttctttt actactactc ctttgcatgt tggagaaatc gattcataat catctctatt
    11821 taccccataa ttacccacca taggataggt aaatgtaatg atttgaccgt taaaactttg
    11881 atcagtgata gtttcttgat aaccagtcat acttgttgta aaaacgattt ctccgaagac
    11941 actagcatct gcaccaaaag cctttccttc gaaaactgtt ccatcttcta atataagcaa
    12001 acgttccata cttttattct cccttctgcc acacaagttt tccgttgaca aaagtcattt
    12061 gtgtttcgcc ttttactttc cagccgataa aaggtgtatt ctcccctttt gataaaaagt
    12121 cttctttatc gatcgtgcaa gtttgtgtaa tatcaaaaac tgctacatca gcgggggcac
    12181 caaccgttag cgttcctgca ttcaaaccaa aaatctcagc cggtttaact gccaaccaat
    12241 tgatcacttg ttccaacgtg aatcgaccag tttctacaaa atgagtatag atcagttgaa
    12301 acgctgtttc gcttcctacg ataccaaatg gtgatttcat gaaactttgg cttttttctt
    12361 ctaatccatg gggggcatgg tctgtagcga tgcaatcgat cgtaccgtca agcaagcctt
    12421 cgatcaaagc ttctctgtcc tcccgtcctc ttagcggcgg gttcattttc caaaagccga
    12481 aatcttcggg aatatcttcg tcgatcaaaa taagatgatg gggtgaaact tctgctgtta
    12541 cgtggattcc tgcttttttt gcgtctctga ttacacggac actttcttta gtagaaacat
    12601 ggcaaacatg gtaatgaaca ccagttgctt cagctagcaa cagatctcta gcaatttgag
    12661 aagactcggt tacgcttaag atcccgggaa gaccaagttc ttctgccttc ttgccagcgt
    12721 gcataacgcc gccaaataat aaagattcat cttctgtatg ggctactagg gctttattgt
    12781 tttttgcagc ttctttcata gccagataca tcgtcccagc tgtttggaca cctacaccgt
    12841 catttgtgaa agcaaaagca ccttcttcaa tcagtgcttc ttgatcaact aatttctcac
    12901 ttcgcagatt ttctgtgatt ggcgcatact gcaatacctt cacaactgca tcttttctaa
    12961 tgatgtcata tacttgtctc agcttttctg ccgtatcagg tacaggattt aaattcggca
    13021 tcgcgcatac agtagtgaaa ccgccacgag cagctgagcg agtcccagct tcgatcgtct
    13081 ccttgtatgt aaatcctggc tctctcaaat gaacatggac gtccactaaa ccaggtgtga
    13141 tcaattgtcc ctttgcatca aacacttcat caaattcggc ttctgagaat cctgtaccga
    13201 ttgctttgat tcgcccatct tctatccaaa tttctgctgg tgtcgtcata ttttttcgtg
    13261 tattgatctg accgttttta atgagtgttt tcattttttc atcctcctat aattacgctt
    13321 ttccatttag cactgcttct aagattgcca ttctgacgaa tactccattg ctcatttgct
    13381 gaacgatccg cgactgtaat ccttccacta atgaatcagc taattctaca tcacggttaa
    13441 ctggtgctgg gtgcataatg attgcatgtt tttgcaactt cttcgcacgc tcaactgtta
    13501 acccatgttc ctgatggtag ccttcttttg agaaactttc ttttccatca tggcgttcgt
    13561 gttgcacccg aagcatcatc atgacatcaa cttgatctag tagttcatcc aatggcatat
    13621 aatgtccgta cacttcgaat tcatcgtcgt accattctct aggacctgag aagaatactt
    13681 gtgcgcctag acgttttaac atctgcatat tggatttagc tacgcgagag tgagtgatat
    13741 cacctacgat ggcaactttc agaccttcaa aatgaccaaa ttcttcataa atggtcatca
    13801 aatcaagcaa gcattgggtt ggatgctgtc cgcttccgtc tcctccattg ataatcgaac
    13861 actggattgt tttactttgt atcagttcgt cataatagtt ctcatcacca tgacggataa
    13921 ccgctacgtc tacgcctaat gctgacatcg tcagtactgt atcgtatagt gtttcccctt
    13981 tttggacaga gcttgtgctt gcttcaaatt caatcacttc tagccctagt tttttctccg
    14041 ctacatcgaa gcttttatgt gtgcgagtac tattttcgaa aaagaggttt gtagcaaagt
    14101 actggcgctt ttgcggagtc caattagcac cttgcttgaa ttcctgccct ctgcggatca
    14161 agcccataac ttcttgatca gtaagtgcct caacggtcaa taaatgcttt aagctgatac
    14221 gttctgattt gatgatcata atgtttcctc cttagttttc ttcgcttcgt gaagtttctg
    14281 gtaagataag atttaaaatg atgccaagta cagttgctaa tgccattgcc gacaatgtaa
    14341 atgtgcctac ttcaaagacg agtccgccaa ttcctactac taagataact gaagcaatca
    14401 acagattttt ctttttatca aaatcgattt tgttctcgat taaaatcttc attccacttg
    14461 cggcaattac tccaaataag ataaaactga ttcctgagat gactggtcca gggatactta
    14521 aaatcaaagc gcttaatttt cctacgaatc ctaaaatgac tgcaaatact gcagctccac
    14581 cgatcacaaa cacgctatgg acacgtgtga tagccagaac accaatgttt tcaccgtaac
    14641 ttgttacagg aggacctccg acaaaggctg ccacgatttg tgctaatcca tcacctgtca
    14701 acgtcttatg aagacccggt tcttcaaaga aattacgttt agttagttta ttcaatacca
    14761 taagatggcc gatatgctct gtcatcgtca caaaagcgat gggtgccatt gttgtgattg
    14821 ctccaagata tagctttggt tcatattgta caaagggaat ctcaaagtta ggaacagcaa
    14881 accaggcagc gtctacgatt ggcttagtat ccacgattcc aaacagcaag gctacgatgt
    14941 atccgctaat aatacctagt aaaatcggaa tcaatccgag aaaacctctt agccacatgt
    15001 tgaagaaaat tgttaatcct aatgtgatca gcgctacagc aatatacttg aaatcatacg
    15061 tgccctgatt atacatagca tttgttgctg catttgccgc aagtcccaac ccgataacca
    15121 tgatgactgg accgacgacg attggaggca agattttatc cagccatgct gaaccgatct
    15181 ttttgatgat cagcgcaaca atcaaataga ctaatccagt tgtgactgct ccttgtgcaa
    15241 tcgctggata accatctgtt ttcatcaaaa gctgcatcgc tgcaatgaaa gcgaagctgc
    15301 ttcctaagta cgcaggtatc tttcctttag tagtaaataa gtacaccaat gtacctagtc
    15361 ctgaactgac tagcgcaata cctggatcga tacctactaa gatggggact aacacggttg
    15421 ctccgaacat cgtgaacaag tgttgtaaac ttaatcctac ccaatgtcct ggggttgggc
    15481 gatcgtggat atctaacacc acgtcatcat tatgaaatcc ttttttctcc gacatgtttc
    15541 tctctcctct aatcttcctc tttcaaaatc atgatgcgat cttgtccatc aagctcttgc
    15601 atctctacta aaatctcttc cgcccgagat gttggaatgt tcttcccaac ataatctgca
    15661 cggattggca attctcgatg cccacgatcg accaggactg ctaatgaaat ttttcttggt
    15721 ctgccaaaat ccattactgc gtccattgct gctcgaatcg ttcgtcctgt atacaaaaca
    15781 tcatcaatca aaataacttc ttttccttct aaggaaactg ggatgtcaga agaatgaagt
    15841 tccggttctt ctggattttc ttttttgtca tctcggtata aagtgatatc cagttctcct
    15901 acaggaatat cgatatcttc tagctgcttc aggcgttcgg caattcttga tgcaatgtag
    15961 attcctcgtg ttttgatacc taccaatacg atatcttgga tactatggtt tctctcgata
    16021 atctcataag taattcgagt caatgctcgc ttcatggtta cctgatctac tacttctttt
    16081 gcttgcattt tcattccccc tacttttttg cataaaaaaa ctcctgccct aagaggacag
    16141 gaggaatgtt tagactgagc gtatactgat acactgatag ctatactatg tcaatgagca
    16201 atctacttat ggcgcaccat acgtatcctt gctcccaccc ttcttagcct cacgggacta
    16261 aacttaaagg aattctatta agttttatgc tttcgttgtt aattactata ccgttatcta
    16321 aattaaaatg caatgttttt tcttaatttt tctaaagttt tttcaaaaac ttctggaaga
    16381 ggtgcttcaa agatcatctt ttcgtttgta gtcgggtgcg taaaacctaa catttgcgca
    16441 tggagaaact gaccattgcc tttcaaagtt ttcttcggtc cgtatactgg atctccagct
    16501 aacggataac cgatatactt catatgcaca cggatctggt gtgttcttcc ggtttctagt
    16561 tttaattcaa ccagagtaaa tccttcgaaa cgttccaata cagtaaaatg tgtgacagcc
    16621 gacttcccat cttctcttat cgcctgcatt ttccggtttt ccttcgaacg gccgattggc
    16681 gcattgatgg ttcctttttc gtgaggaatc tctccatgga ctaaagcaac atatttgcgc
    16741 aaagaggtct tatcttttaa ttgctgagct aatgattcgt gtgcctggtc atttttggca
    16801 accattagca gtccggaagt atctttgtcg attcggtgaa caatacctgg acgaataacg
    16861 ccgttgatcg tagaaagatc cttgatatgg tacatcagcg cattgaccat tgtgccatgc
    16921 gtatgacctg ctgaaggatg aacgaccatt ccttgaggtt tgtttatcac cgctacatct
    16981 tcatcttcgt aaacgatcgt caacgggata ttttctggga ccacatctaa tgtttctggt
    17041 tcggggatca agacagtaat ctcgtctcct tgttttactt tgtaatttgc tttgactggc
    17101 tgtccattga cagaaaccgc tccatttttc agccacagct gaatttgcga acgactgtaa
    17161 tcggtgaata aattagtcaa tactttatcg atcctaccag attcatggtg aatcgtcgtt
    17221 ttaagttctg tcattttttt ctttgatcct tttcatctaa aataatataa attaacatac
    17281 aaatcactcc acatacaagt gtcacatctg ctacgttaaa aatcggaaag ctcataaaat
    17341 ctgtctggaa catatctact acatagccaa gacgaatacg gtcgataaaa ttgccaatcg
    17401 caccagctag aatcaaactc aatccaatca tgaaccagcg attacctttt tcacgatttt
    17461 taatcatcaa tgtgagaatc acaacgactg caatgagtgt cactatagta aagaaccaca
    17521 tcttcccttc tagcatactc catgcggctc cagtgtttcg aatatgattc agtgacaaaa
    17581 tacctggtat aagatttttc gtctctccaa gcgctagatt atcagtgatc caccatttga
    17641 ccaactgatc cactcctact agcaaagcgc tgaatatcca ataaataatt aacaaaaaat
    17701 caatcctttt ctatttgttt ataaatatca cgaggtcgta cgatcccgag cagattacca
    17761 tcgggattcc catctttagt gataagtacg gcttccaaag tcccttgctc acgaaacatc
    17821 gcttcaacct cgtaaatatg tgttttagct ggaacaaaat gataatttgt gtactttccg
    17881 ttttggatga tcaaatccaa cgctcgttta tttgttaaat caaccggacc tttttggctt
    17941 tcttttgcta gccaaaaccc gatattacgt aaagtaatca atgctacaaa ttttccttta
    18001 ttgtataacg gaaattgtga ataccttttt tctgctacta tctttaacag ttcaagtaac
    18061 ggaagatctt gttcaaaacc tgttacacgt tttgcaaatc gaggcagcac ggtttcaggc
    18121 tgaatcagtt cttgttcgat cagctggatt cgcttgactg tccaatcatt tggctctgca
    18181 atgacaaaat cgactgcgat ccgatcatgg acgatcgcat tccttaactg agcaagctgt
    18241 aaaagatcat cttcgtattt tctgatcatt aattgttttc tttgagctag gcgtctgaca
    18301 agttcggtaa atcccatatt tcgtgcattt cccatctcgt ctctcatcca tttttcgatt
    18361 cgattgaaac tgattaaaaa aagatcagag tttgccataa ttttcctcct tgatcaaata
    18421 tgcccggttt tttatataga aaaagaacgc aatcaacaca ataactgcaa aaaaactgaa
    18481 aaataaattt tgtaggaaaa cggcccccaa cccagttgct agaaataaaa acggatagat
    18541 ttgaagaatc aagtaattca ttgtgatcac ataagcaggc tttgcttctt tcactgtata
    18601 aacggggctt tttagccaat aaaaggtgac ctgcatcgta aagggcactt ccagatcaag
    18661 cgttatcttt tgttcatgac tgagaacact gcgtttttca ttgttgattc ttagctgaat
    18721 cgagctaccc ataccgtaaa aaccggtttt tcttataaat gtcgttttca tcataccctc
    18781 ctattatgct ttagtatagc tcaaaataca ataaagaaaa agaatgatcc tagtgtaaac
    18841 aaaattaatt ccctatcgtc taaaaaaaga agctgtagtc aaaatacagc tccttcgatg
    18901 gtagattata atcaagctta aaacaatccc aacacttctc cgtcatttgt cacgtccata
    18961 ttcaatgcag caggtttttt aggcaaacca ggcatcgtca taacatctcc agtcaaggct
    19021 actaaaaatc ctgcccctaa ttttgggatg atttcgcgga tcgtgatggt gaaatcttcc
    19081 ggtgcaccta gcagtgaagg atcatcagaa aatgaatact gtgtttttgc catacaaatc
    19141 ggcagtttgt cccatccatt cttcgtaaac tcttctattt gtttttgcgc ttttttgctg
    19201 aaaacaacac catttcctcc gtaaattttc ttgacgattg tttctgtctt ttcttgtatc
    19261 gtttcttcta ggcggtataa tggcttgtaa ttagcttctt ttctatcaat caatcgtact
    19321 acagcttttg ctaaatcaac gccgccttct gctccatctg cccatacact cgctctcttg
    19381 gcaagaatcc cctgattttc acacaagtgc tccaacagtg tgagttcatt gtctgtatct
    19441 gttacgaatt cattgattgc gacgatcaca ggtagctgat attgttccat attacgtata
    19501 tggcgcttta aatttgcaaa acctactttc aaagcatcaa gattctcatt tttaagttca
    19561 tcttttttca ttccgccatg cattttcaat gcacggacag tggcgacaat caccacagca
    19621 tccggcgctt ttttcaagtt aggtactttg atatctaaaa atttctctcc gccaagatct
    19681 gcaccaaatc ctgcctctgt gacaacgtaa tctgctaatc tcaatgctgt cttggtagct
    19741 aaaatactat tgcagccatg agcgatattg gcaaacggtc ccccgtggac aaaagctggt
    19801 gttccgtaaa ttgtttgtac aagattcggc ttgattgcat ctttcagcag taatgccaga
    19861 gcgccttctg ctttcaagtc tccagcagtc acaggctgac ggtcaaatgt atagccgaca
    19921 acgatccttg caagacgagc ctttaaatct tcaaggtctg ccgcaagaca caagatagcc
    19981 atgatttcgc tggcaactgt gatatcaaac ccatcttctc ttggaacacc ttggattggc
    20041 ccacccaagc caacaattac tttacgcagt tcacgatcat tgagatcgac tacccgtttc
    20101 caaatcactc ggcgggcatc gatgttcaat tcatttcctt gatgaatatg attatctaaa
    20161 agagcagata atgcattatt agctgttgtg atagcatgca tgtcaccagt aaaatgcaag
    20221 ttgatatctt ccataggcaa tacctgtgca tatcctccgc ctgcagcacc acctttgatt
    20281 cccatcacag gaccaagtga gggttctcgc aatgcaataa cagatttttt attgatttta
    20341 ttgagcgcgt ctcctaaccc tacagtgatc gttgactttc cttcgccagc tggtgttgga
    20401 ttgatggaag taactaagat caaatgcccc tcagcgttat cctttgatcg attgattcca
    20461 gaaaaattga ttttcgcctt atatttccca tacagttcca aatcgtcttc cttaagatct
    20521 aatttactag ccacttcaac aatcggttct agctctgttt cttgtgaaat ttgcagatca
    20581 ttcttcattt gattacccct tttcacgaac tttattcaac aaaatatctt ctttgttaag
    20641 atatgccaat tatacctttt tttattcaga aaaaaaacat aaaattacag gtttttaatt
    20701 tatttgacgt acttataaat actctttata atgaaggcta tgatcagact ttagaaaggt
    20761 cgtgagtgct ttgaccacag gaattgtaaa atggtttgac aacaaaaaag gatacggctt
    20821 tatcagttat gatgacacag aagaaatttt tgtacatttt actgcgattg aagaagaggg
    20881 attcaaaact ttggaagaaa atcaagtggt cgaatttgag atcatcgaag ggaatcgagg
    20941 aacacaagct gcccatgtca agaaaggcaa cgttcagact accgatacta gtatgcatga
    21001 atagataaac aaatagagtg agacgctaaa cgggatgaac cgtacgtctc actcttttct
    21061 tttcctgtaa aaattcgttt ttttaggttc tgatttttaa aattagcttt ttcttttttt
    21121 cgtataagga taaggaattg attctgattt gggtccgttt ttttcaaaat gatcaaaaag
    21181 gactacttga tctttttttc gcatcatgaa aacagatggg gctttctcat cttgcaacat
    21241 catttgcacc ccagtattag ttggcgtcaa agatcgaatc tgtccgacag atatttctcg
    21301 gtctttccaa aaagcagcat aacgtataat cacagagttt gattttaaaa taaaagaacg
    21361 ccgcaaacct agaaatagaa aaacaagaaa aatacctaaa acaaaattgc ttttccaata
    21421 aggacctgtg ttttctaaag aaagaatcag actaaaaaac agaatgatca aggtcaatga
    21481 ccaatagatg atcgtatgtg ccagttcagg ctgccatcga atattttttt tcattctcta
    21541 acctctcacc gattcagtaa tgatataatc ttaccataac attagtttga acaaaaagga
    21601 gaatctcttt ttatgttaaa agtatacatt gatgcctcta caaaaggcaa ccctggtcct
    21661 agtggaggtg gaatcttgat tgtctatcaa ggtaaacaag aacagctatc tatcccgctt
    21721 tctataggga caaaccatca agcagagttc gaagtctttt taaaaacttt agaatttcta
    21781 aagaaaaacg acttacagaa tgaaacgatt ttctgttttt cagacagcaa aacactagtt
    21841 tctacggtcg acaaaaacgc aacgaagaac gagctcttcc ttccctacct tcaaagaatc
    21901 caagaacttc tatccgaatt ttctttattg atcctgcaat ggttgcccga aaacaaaaat
    21961 aaaggagcag ataatcttgc acgtcaggct ctccataaac aattaaaaga aaaaaagact
    22021 ctttaaatta aaaactttaa ggagtctttt tatagtttct catttaatat aaaagataat
    22081 tgtgatcaaa cgtaatttcc aattggtaat ctccgatttt ttcttctatt tgactacgta
    22141 agattctttc tagttctgta tcaacaaact ggaatttctc agggaccact acatcaaatt
    22201 gcagcgtttt tccttgatga attactctaa aatcatgaat attaagccct gtacctgttt
    22261 caagaagaat ttgctttaac tgccgcaaga tattatagta tttttcgtta tcaacaggca
    22321 ttggatccaa gtgacagaca agttccacat ccaaagactt tttaaagtct ttttctatca
    22381 gatcaataat cttatgagcc acattcaaat tcattgattc atctacttcg atgtggactg
    22441 atgcaaagcg tttattgggt ccatagttat gcaccaataa atcatgatag ccaagtatat
    22501 catcgcaagc atttaaatgc tcttccattt cttcaatctc tgctggcgtt gggcgacttc
    22561 ctaatagttc gtatacaaaa tttcgaagca tcgtcaatcc actataaagg atatataatg
    22621 ctaaagcaaa acctatatat ccatcaatac gccatccagt tacccattcg attgctgctg
    22681 aaagcaatac agccagtgta gtaaagacat cattataact atctgttgct gtagctctca
    22741 atgtttcaga atcaatggat tgcgcaataa cctgatacat tttgccttgt gcaaatttca
    22801 aaaagatgga aatgattagg acaaaaaaga cgatgggtgt taaagagaca ttttctggat
    22861 ggatgatttt ttcagcagaa gactctaaaa attgaaatcc tacataagta atgatgatcg
    22921 aaacaaataa cccactgata tattcgaagc gttcgtgtcc ataaggatgt tcctgatctg
    22981 ctggctttgc agcaatttta aatccgatca aagtcaatat ggatgaagca gtatctgata
    23041 gactgttcac tgcatcagcc ataatggaga cacttcctga aaaaagcccg atcattagct
    23101 tagagacaaa caagatcaga ttcgtaacca atcctaagat acccgctagt actccttttt
    23161 ttgtccgctg ttgcggtatt tgtttctttt tttcttcttg tttcttagcc aagtttttta
    23221 ccccttctag tttgcttgtt gctcaggtaa aaagatggag aggacgattg ttttcctctc
    23281 ctctcttcct ttttctatct atcgtgcgtc ctattactat taaaagatgg tcattattct
    23341 ggtttcaacg gtgttgctgc aaataacgct tttgctagta atggtgttat gattcccgca
    23401 cctattgctt ctggtatccc attgattgct gcaacccctg ctaaggtttt aaataaaaga
    23461 gcagagtcta caccataggc atctgccgtt gcacctgtat aaaataatcc catgaatcct
    23521 aacactaaga ctgtattgac caatgaaccc agtacccctg caaaggctga gctgatcgtt
    23581 attgatttat ttttttgata aatccagtgg aaaaccaagc ctgctactaa gccaacaagt
    23641 actctgggaa ctaccgagat aatcggattc gtgaacacta atgtatcaaa tggcgttgtt
    23701 ggcatggcat aggcacgaac aattgtagcg attccccaaa acaagccaat caccatgcca
    23761 tcttttgggc ctaatactac tgcagcaata attactgtga tatggataat tgttaaacta
    23821 atgaatccta atggaataaa ccccagccaa ggaaccatcg cttgtacgat gatgattgct
    23881 aacaagatcg cacgtaagac tagtcgaaag gtcttatttc tactattcat tttttcaccc
    23941 ctttttattt atactaatcg taaaaatttc tgtcactttc gtcaactaaa gaccatccga
    24001 atccgaccat ttgattagat gcgccatcca attgaaaaaa gtatgagggc aagacttgtt
    24061 tgtcttcgcc cccataatca tgtttcgttc aatgtttgaa cttttgtttt tctgtcaaac
    24121 gtaaacgccc tattgttgcg caacccattc tttcgcagct ttaagtgcat ctggaatacc
    24181 agcaggattt ttcccgccag cttgtgccat gtctggacgt ccaccaccgc caccgcctac
    24241 tttcggtgcg attgctttaa tcaagtcgcc agcttttagt ccagcttcat ttgattcttt
    24301 cgtcattgcc gctagcagac tgacttttcc ttcttgttcg tttgctaaaa caagaatatc
    24361 tgaaatattt ttttgtttcc attggtcagc taattgacgc agctgattca tatctttcac
    24421 ttttacttgt gctgcaatga ttgtttttcc attgatcgtt tctggatctt tgaatacatc
    24481 accagcttgt tgattcgcta acttgctagc taattgttca ttttcctttt gtagctctct
    24541 tacttgctgt tgcaattgtt ccgcttttga aacaacttct ttcaattgag gtgatttaac
    24601 aattgttgct acttgtttta gttgtttttc ttcttcttca agtagttcgt acgcttcttt
    24661 acttgttaca gcctcgattc gtcgcacgcc tgcaccaatt cctgattcag aaacgatttt
    24721 gaagattccg atgttttctg tatttgctac atgtgtgcct ccgcacagtt cgattgacca
    24781 gccgccaata tttactactc gaacgtcatg tccgtatttt tcaccaaaca aagccattgc
    24841 acccatgttt ttggctgtat cgatatctgt ttcaattgtt tcaactggga tagcttccca
    24901 gattttttcg ttgacgattc tttccatttc agctaattca tctgaagtga tttgaccaaa
    24961 gtgggtaaag tcgaatcgta aatgtccagg agcgactaaa gaaccagctt gatttgcatg
    25021 ctcgccaagt acttctttca atgccttgtg cagtagatgg gttgctgtat ggtttttgat
    25081 gatccgattg cgcatcgatt gatcgatcaa tagatgatag gtttgtcctt cactcattgg
    25141 tgcttttact tctacggtgt gcataaattg accattaggc gctttttgaa catctgtaac
    25201 aactgctacg atttcgccta cttcattcat gatccatcct tgatccgcta cttgcccacc
    25261 catttctgca tagaaaggtg tttgatcaaa gatcatttga gcttcacctt ctgcgacttc
    25321 attcaaaaga gactcacctt taacgatgac caataattga ctatctgatt cttgttctgt
    25381 gtatccaatg aatttacttt ctactttgat atctgtcaat aaagcagatt gtacccccat
    25441 tgatttttca gtactgcggg cagagcgagc acgttcgcgt tgtgcatcca tttctttttc
    25501 aaaaccagca tgatcaactt tcagtccttc atcttctgcc acctcttccg ttaattctac
    25561 cgggaagcca tatgtgtcgt atagtttgaa gatgtctttt cctgctagag tcgtcccgtt
    25621 ttcgtttttt acttcttgga tcacttgatt taagatttcc aaaccttcat tgattgtttc
    25681 attgaaacgt tcttcttctg tacggataac tttttcaata aattcttttt gttgaagtac
    25741 ttcaggataa taacttacca tgatttcccc aacaacaggg actaaacgat ataagaatgc
    25801 ttgttcaata cctaattttt tcccatgcat gacagcacga cgaagcaaac ggcgtaagac
    25861 ataccctctc ccttcattcg atggcaatgc accgtcgcca atagcaaatg aaagggcgcg
    25921 aatatgatct gcgattactt tgtaagaaat atctgttact gggttttcac catattttac
    25981 ttgtttgctt agcttttcta cggcgtggat aataggcata aacaagtctg tctcaaagtt
    26041 cgttgacgca tcttgaacga ttgagaccat gcgttccaat cccatccccg tatcaatgtt
    26101 tttatgagga agtggttcaa atgtgttatc aggcttatgg ttgaattctg aaaatactaa
    26161 gttccagatt tctaaataac gttcattttc tcctcctgga tagttttcag gatcgtcttc
    26221 tgctaaatcg ttataagctt ctccacggtc atagaaaatt tccgtatccg gaccacatgg
    26281 gcctgcaccg atatcccaga agttgtcttc tacatcaata atgtgatcta gtggcaggcc
    26341 aacttcttca tgccagatgc gttttgcttc tgtatctttt ggataaactg ttacataaag
    26401 tttttctgga tcgaatgcca tccattttgg atcagtcaaa aattcccatg cccagtggat
    26461 ggcttcattt ttgaaatagt caccaatcga aaaattaccc agcatctcaa acatcgtatg
    26521 gtgccgtgct gttttaccga cattttcgat atcatttgtt cgaatagatt tttgggcatt
    26581 tgtgattctt ggattttctg gcacaactga accatcaaaa tattttttca atgttgcaac
    26641 accagagttg atccacaata aggttggatc gttcacgggg atcaaagacg cactaggttc
    26701 aatcgtatgt cctttagatt ggaagaaatc aagaaacatt tgtcgaactt gtgcactact
    26761 taattgtttc atgtcattac ctgctttcta taaattaaat acaaaaaaac accatcacag
    26821 ccaaggacga aaactcgcgg taccaccttg attgcaatga tgtgtaatca ttacctctta
    26881 aactcgataa cgctgagctg cgtcggtatt tctaccaaaa tagaaaaagg gagcaatttg
    26941 attagtgact attctttttt cagccaagaa agaattttct gtgagtctta aaaaacaaat
    27001 catatccttt tcgtacttaa ttagtatacg cattctattg atcactgtca atatctcaat
    27061 ttttattttc atttcctttt ggtcatactc cacgattttt ggtacgataa gaataaagga
    27121 atcaatacat ctctgctatt catgacaagg aggaatatga tgaaaaaaat agttggagcg
    27181 gatgaatgga tacgcgcagc tagctgcttt ttgagatatc aagtttttgt attagaacaa
    27241 gggattcctg cagaactaga attcgacgga aaagatatcc cgcaacaaac ctattatcta
    27301 tttcttgatc aaaaaagacc agtagctact ttgcgttatg aaaagaaaac acctacttgc
    27361 ttcaagccgg aacgattttg cgtagcaaaa gaataccgag gaaaagggtt aggcggtcag
    27421 ttgcttgaag ctgctgaaaa acgggcggtt tcagaaggtt gtcagactag ttatgtagta
    27481 gctgaaatga cggctgtaga tttttatgaa aaacaagggt atcatacttg cacagaacct
    27541 tttattgaag atgaagtact ttgtatcgga atgaaaaaaa gtctagctta aaaacaagag
    27601 attgtgacac gccttttgtc acaacctctt gttttttatt tatgtgaatt tttcttattt
    27661 ttacgagcag tacggctttg ctgtctgcgt tcgattttgc gttctttttt atttttttct
    27721 gcaattgccc aatcgatttt tttcttataa ccaggcttga tctttttctt tttcttcttc
    27781 acaagaccaa tcagcgtggg atctaataca tcttttgatt tttctcgttt ggcacgtcta
    27841 ttacgatcgt atgttttgac gatttccccg tttttgattt cttttggttc aaatgagacg
    27901 cccaattttt ctatcgcatc aatggcttgc tcatcatttg gcgaatataa cgtaatggcc
    27961 gttcctttta agccattacg tccagtacgt ccgacacgat ggataaagaa gtctagatct
    28021 tctggaattt ctgcattaat aacatgagag acaccttcga tatcgatccc tctagcagct
    28081 aaatcagtcg ctacaacata ttgatactcc agattctgta cttgtcgcat cactcgtttt
    28141 ctctctcttg gggtgatgtc tccatgaatc tttgctactt tcaagccttg gtttttcaag
    28201 taatcggcaa tttcatccac atgctgtttt gtattggcaa atacgattgc taaataagga
    28261 tgaccaattg tcagcagttg ataaatcatt tgattctcat ctttaccttt tgttgaaatc
    28321 agccaatttt caatgtcttc agaaataact gcttttggtt tgatttcttc aatgattgga
    28381 ttttccatgt attttcgtaa gaatggacga agtttttctg gaatagtggc agaaaaaact
    28441 aaaaactgca gcttttctgg taatcgactt gcaatttggt ctacttcatt taaaaaaccc
    28501 atatccaatg tcatatccgc ttcatcgaca acaaatgcaa atgcggtgtg aatcttcagc
    28561 gcttgttcat tcatcatgtc taatattcta ccaggcgtgc ctactacaac ctgtggctgt
    28621 tggtgatgca gacgatttaa ttgacgctgt ttgtctgtac cgccgacaaa attcgagaca
    28681 cggatctccg gtgtagaaaa tttagcgatt tgtgtagctg cttcatagat ctgtgcagct
    28741 agttccctac ttggagctgt aataacaact tgtacttcct ctttttctgc attgattttt
    28801 tcaaacaaag gcaacaaaaa cgtatgtgtt tttcctgtac cagtttgaga ttgcccaatg
    28861 atattttttc cttttgcaat taaaggaatc aaacgttcct ggacctctgt gggctgtttg
    28921 aagccctttg cggttaacgc ttcattgata aacggtttga agcggaatat atcaaaatta
    28981 ttcattcgtg cacctcttct ctttaaatta tcacgacttt ggcattataa cacagacaaa
    29041 gcgtttttgc tcagctaatt tcgagaatca tcatcctttg tttggccaat cgtgctaaca
    29101 gtatatgtgt gactagcttt ttcataaaac agaatccgtt tttttctcaa aaatgatagc
    29161 aagatattta aaataaaaat accttgtagt aacaagacac cgagagaaat gaatactgca
    29221 ctcatctgca acaccgtatt cgttgtcgaa ttcatgattt gctgaagcaa ctgccccaac
    29281 cagcttaaac cataaaaaag aaagtacaga tacccgtagc ggatcatcaa tgcttttaaa
    29341 gaaattcgtt cgcggtcttc ttccaccaaa cggatacgca caattttttt gccaagtgtc
    29401 catccattgg ttataaacgg tacaatcata aagtataccc ctacttctaa taaaagccac
    29461 cagatatgag aagtaactgc ccctgaatcg aacgcaattt taaagaaaat cgccgctacc
    29521 aaattaataa gagataaaaa tgtccagtcg atcaaataag cgaccaagcg tcgagtcaat
    29581 gagacagtct ttcctttttc ataagaaagt tcatccattc gtcttcttgt aggaagcata
    29641 aatgtaaata acggcgtaac gacaaaacca atgattccac ccaatgtatt tgtcactaaa
    29701 tcattcacat caaataagcg gtatggtctt gggtagataa aataaagtcc acttagctgc
    29761 gtcaattcaa aaaatagaga aaggcagaaa ctagcaatca tcgttttcat aaaagaaagc
    29821 cggaaataat atcttaaata aatgccaaac ggtatgagta ataaaacatt aaaagctggt
    29881 tccaaaaata cactctgctt cattgccggc aggtaggtac taggttcaga caagaccaat
    29941 accgtgttgt ctctgaaatg ttgccatgaa gcaaataaat gcagctccat ctttggccct
    30001 gtcatttggg caaccgtctc ctttgccgga agtggtaaga tcaccaaaaa atacgcacac
    30061 aataaataaa aaacgaaaga atacaaaata actgcccttg ttactagaaa tgttccatac
    30121 tttcgatatt cataaataaa aaaaacagat gagatcgcta aggctaaaaa tggaaaaaca
    30181 atcagcgcaa aacgaatcgg ttcgatataa cttgccatat gactcctcct tgattctttg
    30241 tatctctctt atcttagcag tcctttgctg tttcaagcaa gtaacgaggc tattatttct
    30301 catttctttc aaagtaaagt aagataaaca caaagggggt gtcgagatgc tgaatatgta
    30361 ctttgttttt ggcgtaccta tttttttact gttcctttat gctacgatcg cttatgtaag
    30421 aaaaagaact acaattcatt atttaggatt tattctcctg attatttcgg gtttcatgct
    30481 agtcttcaat cttcagactt ggcagcaagc acttcttgaa atggataaaa tgactccaca
    30541 tgctttgtct aaagtgcttg gctaccctgt ctatctgatt tggcttccca ttttcatttc
    30601 tggttgcttg gttttattaa atatttatcg cggcgtacgc cgaatagttc agttacgcaa
    30661 atcaaagtaa aagacagaaa tcggttgagc ccgatttctg tcttttactt tgctatttca
    30721 tcgtagacaa aacgacattt acgtgatcaa aattaacgag tacttttgac atttcttcca
    30781 cgtactcctc atttaaataa aatattttgt ttggcagaga agtgcagttc taataaaatt
    30841 cgaatgtttt agctatctac gttttaaata gagataaatc gggataccag caagtgtcaa
    30901 tactatcccc atcgaagcta aaaaggtctg agtgataagc gtgcttgata cgataaaagt
    30961 ccctccaatg attgctacta gtggaataaa cggatacatt ggaactttgt atggtcgaaa
    31021 caaatcaggt tcttttttac gaagaagaat gacaccaaca aagaccatcg tataaaaaat
    31081 ccaaatgaca aagatcagca tatccgttag tgtatcaaaa ccaccgatca tcatcatgcc
    31141 aatagcgatg actagctcta agatacctgc ggctacagga actttagttt tatcatgaag
    31201 tttagcaaag agcttgctaa aaggcaggtt gttttctttt gccataacat aagggagacg
    31261 cattccagta agggtatagc cattgatggt tccataaaca gaaatcaaga taccaactgt
    31321 gaccagtctt ccaccgaagc cgccaaaaat catttttgca acgtcacttg ccgcattgct
    31381 gtttcctgcg actccgtcga tagatgcggt tcttaagaac accgcattta ctaataaata
    31441 aacgatcatg attccgatga ttcctaaaga aattgctttt ggcaaatctt tcgccggctt
    31501 tttcaattct cccgaaatat ttcctacgtg tatccaaccg tcgtatgcga acatcgttgc
    31561 tagcaagccc gctcccaagg ctgagaaaaa tgaaagattt tctcctgctt ggatcggaaa
    31621 cagttggaag tctactcctt cttgtcggaa caaaccgaaa atgacgatca caaacaaagg
    31681 aataagcttg cagacaagcg taatcgactg aaaagcccct ccagctttag aacctaaaaa
    31741 attgatcaac aggatcgata cagcagctgt aacagctacg ggaacaatca ttgactggga
    31801 aagaccaaat agattgacga actgtgtacc gaaaatgatc gacaaggctg ctacatttgc
    31861 tggaaaataa atcacgactt gcgcccagcc caataaaaaa gctgctgtat ttccgtaaat
    31921 ccgttcgata tatttaatca tccctcctgt ttctggaata gcagctgcca gttcagctcc
    31981 tgttagtcct gcacagacag aaatcatacc accgagaaac caagaaaaca tatgcaaact
    32041 ggcggatcct gtaacttctg ctacgcttgc ggctttaaaa aatacccctg caccgatcac
    32101 tgtgcccata acagtcgata aagcagtaaa aaatcccatc gtacgtttta attcattttc
    32161 tatttttact tcttttactg gcgtggcttg atattcttcc atcccttgtc ccctttcata
    32221 ccatagccaa tcccaaaagt ataaaaaact ttttggatgt ggttcttgtt ttataccgtc
    32281 tctaccaatt tttctttcag tttttccaac atatcctttg tcatcacatc taagtcgtat
    32341 ttcggcgaaa agccccattc ctcttgcgca caagaaacat ctagtgaatt tggccaagat
    32401 tctgctattg actgacgtac aggatccaca tcataatcca tttcaaaatc tggtatgatt
    32461 ttttgaatag atgctttgat ctcttccggg tcaaaagaca tcgccgatat attgaaggca
    32521 tttcgatgaa ccaatcgatc aggatctgca ttcatgagtt ttataatggc atcgatcgca
    32581 tctggcatat acatcatatc cattgatgta ccttgatcaa tgaaactagt gtactttcct
    32641 tctttgattg ctgaataata aatatcgact gcgtagtctg tcgtaccgcc tcctggcaag
    32701 gttgcataag aaatcaatcc agggaaacgg acaccacgag tatctacgcc atatttcaca
    32761 taataataat cacatagtag ttctccagct accttcgtta ccccatacat ggttgtagga
    32821 cgctgaagag tatcttgtgg ggtatgatct tttggagtat ttggaccaaa cgcgccgatt
    32881 gaacttggcg taaaaaattt cagctggaat tctctagcga cttccaatgc attgaccagc
    32941 ccagtcacgt tcaaattcca ggctaattgt ggttttgctt ctgcaacagc tgacaaaaga
    33001 gcagccaaat gaatcaatgt gtccacttga tattgttctg ctatgctgcg cattttttcg
    33061 taatccatta catctaggat ctcaaagata tcgtttttct caatgacttc aggcatgcgt
    33121 atatctgtcg caatgacatt ttcactgcct agttcttgac gtagccgtgt gatcaactct
    33181 gaaccaattt gtcctaaaca tcctgtcacc aatacttttt tcatgatgat cctccctgat
    33241 cttttttact actatcttca gatgattaac aatatatgcg tgaacatgtt ctctaatttt
    33301 aaatagacga aaaaactaaa tttagttaaa taatttccat ttcttttcct attttttgat
    33361 aaactgctac tgcttcatca agcatttccc tcgtatgttc cgcagtcggc atatttctga
    33421 tcctgcctgt cccaagaggc acagttggat aaacgattgg tttagcataa atcccatgtt
    33481 cgatcagctt cttagagaaa gtctgagtca ctttttcgtc tcccaaaatg actggagtaa
    33541 taggtgtttc agacataccg atgttatagc ctactttttt caattcttct ttaaaataat
    33601 tagcattttc ccaaactttt tcgacaagtt ccggatgttc ttgcatcaaa gtaatcgaag
    33661 ccaatgcagc tgcagctgca cctggagtta gcgaagtaga aaataggaat ggccgacttc
    33721 tagctttcaa ccagtcgatt agtgttttcg atccagctac ataaccccca acaacaccta
    33781 tagctttcga taacgtgccc atttgcatat cgattttgtc gcttaatcca aaatgcttga
    33841 cggttcctgc accttttcca gttacgcctg agccgtgcgc atcatcgacg taggtgatca
    33901 gtccatattt ttcaacaatc ggaataattt ccggcaatcg tgcaatgtct ccatccatgg
    33961 agaaaacacc atctgtgata tacatgattt ttttatattt cttactttct atcgcttcct
    34021 ttgctttttt ctcaaggtct ttcatatctt gatgtttaat tcggatgatt tttgctccgg
    34081 ataagcggca accgtcaata atagatgcat ggtttaattc atccgataag atagcatctt
    34141 cttttgtcat tactgcagag atagcgccca tattgcagtt gaatccagat tgaaaggcga
    34201 ttgctgcttc tgtccctttg aattctgcga ttttttcttc taattgctga tggattttca
    34261 gcgaaccatt gatcgttcgg actgctcctg ctcctacacc ataagtttcg gttgcttcaa
    34321 tacatgcttt tttcaattct tcgcgattgg caaatccaag ataattgttg gatgctagat
    34381 tgatcatctt ttttccgtct acgatgatac aagcaccatt ttcactttct agtatgtcga
    34441 tcgtattgta taaccccttt tctttcagtt gatccaattc tttctctaaa tacattgtta
    34501 gttctttgga agacattgtc tccacctcca tattcatctg tcttgcagaa cataccaaac
    34561 atatatcgtg tcattcatct acaaagcaag tatgctatat ttttgataac gcttacactg
    34621 attatgagaa ttatttaacc acatttttta tttacagtat ctttttactt tcaaatgact
    34681 tatatagtat gaacaattac cttttagata atgatctatt ttactctacc tgggcagata
    34741 aatataacac aaccattgat agaagccctt tcgttaaaat caattgtatt ctgaatattt
    34801 aatttattta ttgcatagac caataaaaat acataaaatt tccatcttcg tttatcagca
    34861 atttttaatt ttactctctg aatatttctt aaaagtttta cttgttgaat tcgaaaaaag
    34921 gactgtgaca aaagtcttgt cacagtccta ttcttccgaa
//
LOCUS       DADVXU010000014        34771 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.015, whole genome shotgun sequence.
ACCESSION   DADVXU010000014 DADVXU010000000
VERSION     DADVXU010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 34771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.015"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(227..1114)
                     /locus_tag="KB953_000732"
     CDS             complement(227..1114)
                     /locus_tag="KB953_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBB6865728.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(1111..1980)
                     /locus_tag="KB953_000733"
     CDS             complement(1111..1980)
                     /locus_tag="KB953_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBB6865729.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(2117..3403)
                     /locus_tag="KB953_000734"
     CDS             complement(2117..3403)
                     /locus_tag="KB953_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBB6865730.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            3676..4254
                     /locus_tag="KB953_000735"
     CDS             3676..4254
                     /locus_tag="KB953_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBB6865731.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            4254..5183
                     /locus_tag="KB953_000736"
     CDS             4254..5183
                     /locus_tag="KB953_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6865732.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            5180..6916
                     /locus_tag="KB953_000737"
     CDS             5180..6916
                     /locus_tag="KB953_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBB6865733.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(6979..7815)
                     /gene="pheA"
                     /locus_tag="KB953_000738"
     CDS             complement(6979..7815)
                     /gene="pheA"
                     /locus_tag="KB953_000738"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HBB6865734.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(7812..8321)
                     /locus_tag="KB953_000739"
     CDS             complement(7812..8321)
                     /locus_tag="KB953_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HBB6865735.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(8314..9609)
                     /gene="aroA"
                     /locus_tag="KB953_000740"
     CDS             complement(8314..9609)
                     /gene="aroA"
                     /locus_tag="KB953_000740"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBB6865736.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(9622..10713)
                     /locus_tag="KB953_000741"
     CDS             complement(9622..10713)
                     /locus_tag="KB953_000741"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HBB6865737.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(10730..11896)
                     /gene="aroC"
                     /locus_tag="KB953_000742"
     CDS             complement(10730..11896)
                     /gene="aroC"
                     /locus_tag="KB953_000742"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBB6865738.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(11898..12962)
                     /gene="aroB"
                     /locus_tag="KB953_000743"
     CDS             complement(11898..12962)
                     /gene="aroB"
                     /locus_tag="KB953_000743"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBB6865739.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(12995..14017)
                     /gene="aroF"
                     /locus_tag="KB953_000744"
     CDS             complement(12995..14017)
                     /gene="aroF"
                     /locus_tag="KB953_000744"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBB6865740.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(14676..15650)
                     /locus_tag="KB953_000745"
     CDS             complement(14676..15650)
                     /locus_tag="KB953_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HBB6865741.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(15631..17514)
                     /locus_tag="KB953_000746"
     CDS             complement(15631..17514)
                     /locus_tag="KB953_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HBB6865742.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(17532..19757)
                     /locus_tag="KB953_000747"
     CDS             complement(17532..19757)
                     /locus_tag="KB953_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBB6865743.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            19872..20906
                     /locus_tag="KB953_000748"
     CDS             19872..20906
                     /locus_tag="KB953_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBB6865744.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(20973..21986)
                     /locus_tag="KB953_000749"
     CDS             complement(20973..21986)
                     /locus_tag="KB953_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBB6865745.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(22064..22252)
                     /locus_tag="KB953_000750"
     CDS             complement(22064..22252)
                     /locus_tag="KB953_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HBB6865746.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(22692..22820)
                     /locus_tag="KB953_000751"
     CDS             complement(22692..22820)
                     /locus_tag="KB953_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBB6865747.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(22823..24196)
                     /gene="rlmD"
                     /locus_tag="KB953_000752"
     CDS             complement(22823..24196)
                     /gene="rlmD"
                     /locus_tag="KB953_000752"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBB6865748.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(24307..25362)
                     /locus_tag="KB953_000753"
     CDS             complement(24307..25362)
                     /locus_tag="KB953_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBB6865749.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(25372..26802)
                     /gene="gatB"
                     /locus_tag="KB953_000754"
     CDS             complement(25372..26802)
                     /gene="gatB"
                     /locus_tag="KB953_000754"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HBB6865750.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(26799..28271)
                     /gene="gatA"
                     /locus_tag="KB953_000755"
     CDS             complement(26799..28271)
                     /gene="gatA"
                     /locus_tag="KB953_000755"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HBB6865751.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(28271..28576)
                     /gene="gatC"
                     /locus_tag="KB953_000756"
     CDS             complement(28271..28576)
                     /gene="gatC"
                     /locus_tag="KB953_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HBB6865752.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(28682..30718)
                     /gene="ligA"
                     /locus_tag="KB953_000757"
     CDS             complement(28682..30718)
                     /gene="ligA"
                     /locus_tag="KB953_000757"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBB6865753.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(30735..32969)
                     /gene="pcrA"
                     /locus_tag="KB953_000758"
     CDS             complement(30735..32969)
                     /gene="pcrA"
                     /locus_tag="KB953_000758"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HBB6865754.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            33124..34683
                     /locus_tag="KB953_000759"
     CDS             33124..34683
                     /locus_tag="KB953_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HBB6865755.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
BASE COUNT         9518 a         7494 c         6336 g        11423 t
ORIGIN      
        1 gacaatcagg acagtcatcg gcaatatagc agcaatcact tctttaaaat tttttagcat
       61 gtatcctcct cctttactct ttaagtttac cttaaaatga aagagttttc gagcacaaac
      121 ttaaaacgtt atcatttaat ttttctcaac ataaaataac acacgtccca aaagtatctt
      181 tttacttgga aaaatgactt tgaaggaacg cgcttattgt gtttttttat tttacagagc
      241 cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa tacgatggca gttggtaaag
      301 tagcgatgac taatccagcc atgatcattc catagtctgg tgtataggaa gcacccatag
      361 cagataaaac aagcggaacc gtccgtttat caggagattg caaagctact aagggccata
      421 aataattgtt ccagctactc atgaatgtga tgattgcagc cgcggcatag gtatttttag
      481 ccgtcggcat atacactcta aaaaaaatac ttagttcttt tagaccgtct aatcgagcgg
      541 cttctactag atcttttgga aaagatttaa cattttgtcg aaagaagaag atcaaaaaag
      601 cagtgctaac agatggcagg atgacaacca agaaactgtt gattcccaaa accgttccgt
      661 tcaattgtga aaacattcga tataaaggga tcatcaatgc cgcaaaagga accatcatcg
      721 atagcagtag aatattgaaa attaggtctt ttcgtttact ttgataaatc tcaaatccat
      781 atcctgccat tgacgaaatc agcaatgcaa acacagtggt caccaaagcg atcatcgctg
      841 aattccaaag ggaacgagta aaattcagct cattggaaaa caaattttga aaattgatga
      901 tcaagttact gccaatcttt atttttcccg atgtgatatc cacgggtgta ttcgtcattc
      961 ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga taggatgctt aatgctgtat
     1021 actttaatac tgacccaatt gtcgaacgaa gttctaatgc ttgttttttg acgttggggc
     1081 gttcgattgt ttctttttct accatatttt tcatcgactt tctcctccta ctttcgtttg
     1141 gattatcgac aatatcacaa taaaggaaac aataacaaag gaaactgctg ccgcatatcc
     1201 aaaattaggc gtgaatttat aactcaaatt atagatgtat tgagataggg tggtcgttgc
     1261 atttgctggt cccccgccag taatattttg tacttcatca aaaagctgca atgtcccgat
     1321 cgtcgaagta atggaagtaa acaaaatgat tggttttaaa tttgggatcg tcactttgat
     1381 aaactgctgc catttagaag caccgtcaat agatgctgct tcataaatct ccgaatcaat
     1441 attttgcatg cctgaaagga aaaaaatcat attgtatcca gtccaacgcc aagtgatcga
     1501 aagaatgatc agaactctcg cccaaattgg atgagtcaac cagccaatcg gtgtgtcaat
     1561 cataccaatg cccaacaaca agttgtttac aaggccagat tgagaaaata gacttttcat
     1621 gatcagagaa taactgacca tcgaagtgat acatggaaga aagatagcgg ttcgaaacaa
     1681 ccctttatat ttcaactttt tatcatttaa aaggttggaa ataaaaagtg ctagaaacaa
     1741 catgatcggt acttgtacaa gtaaatacag gaatgtatta aaaaaagctt ttttaaaagt
     1801 tgcatctgtc aacatccgct gataattacc taacccgtta aatgtcatat tgacaggggg
     1861 accactttga aaagacgtga tcaaagccaa aaccatcgga ataaaaacca tgattgatat
     1921 caaaatgact ggcaagatta aaaaagcatg cccgtttttg aataaatctt tctgcttcat
     1981 tcttttccct ccaattctat cattgattat ctacatgaaa agacaagaaa gacaatatct
     2041 gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg atgaaaaagc gaagaaattc
     2101 acttttttac caccgatcaa ttagccaatt gtgcttctac ttgtttttgt gtatctgcta
     2161 gtaccttgtc tgtctcttct ccattgatga ttcgatgaag agcttcagct acgactgctt
     2221 caattgcata ggtctcatgt ccgtaattca cttctggtat ttcagatgtc catgttgaaa
     2281 aatcgtcaaa gatcttttga ccactgtaaa attcagaagc ttcttgataa gcttcttgtt
     2341 cattcgcgct aagcatcgta gatactagtc cgatttcttt cgcaagagtg cccatcagtt
     2401 cttcattcga agcaaacgtt gcttctagga aatctttcgc tgaatcttct ccagctactc
     2461 ctttgatcac ataccagcca gcgccgccaa ggttagaagc ttgcgctttt tgcagatttt
     2521 ctggaagtgc aggtattggt gcaatcttcc attttccaga ttggttttct gctccttgga
     2581 tcgttgaaga ataccaagcg ccagttgggc tagaagctac tgctcctgat tgtatcgcag
     2641 ttactccagc attccagtca gaggtttgtt cgactagatc ttctttcaaa agagtggcaa
     2701 acaattcaag accatacttt aaagatgcat tgtcttggat cgtgactgtc tccccatctt
     2761 cagatgtata ccattctcct gcctgttgca taatcatcct tactcgacca aggtcagaag
     2821 gattgacttc agcgattttc tttcccgtct tttctttgac atctcgcgct acttgaatat
     2881 actcgtccca cttcagattc tccatatcct cttctgtata acctgcttct gccatcagat
     2941 ccgtacgata gaagtttgct gttacgcccg aatcgaaagg aacaccgtat attttatctc
     3001 ctactttgtt taccgcaaat ttatacggtg caaaatgatc ttcctttaca atagcagaca
     3061 aattagcgaa tgcatctgga taagaagtca aatagccttg tatcctataa tcttcaatca
     3121 agacgatatt gggcaatcct tctgtattcc cgctcgctaa agcggtgttc agtttctgaa
     3181 cgatatcatc ctgagacatc gtaacaactt ctatctctgt ttcttcgttc tcataaactt
     3241 tttttgcttc attcactgct ttgatattga atgtctcatc ccacgcccag acagtgactt
     3301 tttcttcttt gtctgctgta ctactagcac ttccgcatcc tgttaagaaa cctgatgctg
     3361 ccgctaaaca aacgactcca gcaattaact ttctgatgtt catttttatc cgctcctttt
     3421 ccatccatta gttgtttacg tttgctacat tcatcatacc aaaatttgaa aacggtttct
     3481 ttattattca gatacttcaa aaatttctcc taaaataata aaaaaataca aatcaactat
     3541 gacaaaagga ataatcttag catctagacc aaagatcgaa atggtgaggt ttctttaaaa
     3601 taaaaatatc cttgcatttt ctcctagaaa taagtatttt taaattacaa gactattctt
     3661 aaggcgggta aaaaaatgcc aatacgcgta attcttatcg atgatgaaca gacgatttta
     3721 gatggaatga aacagttgat caattggtct gagcacgggt ttcaattaat agggactttt
     3781 tccagtccgc tgaaagcgct tgatttttct ttggtacatg caatcgatat cgtggtcaca
     3841 gatgtaatga tgcctgaatt aaatggaatc gaattatcgc ggctattaaa acagcaacaa
     3901 cctgaaacac agatccttat cctctctagc tatgatgaat ttgatatggt taaagattct
     3961 tttaaagaag gcgtcgctga ttatctttta aagcctcgat tgagtcctga tttttttcta
     4021 aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc ccaacaaaag cccaatcacc
     4081 aatcgttatg agcagatctg cgagaatctt tcacagcaga ttgctggaca aactcgtcca
     4141 ttgcttgacc ctcttctttt tgaacacacc ttgtttttct gttatatacc gaaaaacggc
     4201 cattcagttc aaaacaaggc tctaaacaaa aacagtcgat cacccattca ttgatggata
     4261 gcgacgattc gtttactatt tatcctgttt ctaccttttc ggaagaatcc ggctatgtaa
     4321 tcaatactga ttccaaagat acacttagga agtttgttca aaagtttcgt caagaagttt
     4381 ttccaggtga tttttttgtt gtttctgatt cttttgcaat ccaagatatc aatgaaatat
     4441 tccaagagat cagaaattgc accaaaggac aacgtttcta tcataaggga aagaaccttg
     4501 cttatcagca tgaacttctt ccgttattaa ccaaccacga gcagcaagca agtagttatt
     4561 taacaaaaat cgttcataaa gactacgctt tagctgtcaa agaacttgag aatttcttca
     4621 cgacgatatt ggaacagcct attgatccag cttatttgaa acatgaggca atcaatcgtt
     4681 tctatgcatt actcaacgct ttggctgacg agcatgttgg gaatgaagaa ctttctttgc
     4741 tcaagatcgc ccttccttct ttattagccg atgctgagta tttcgaagat ttttctattc
     4801 ttgtcaaaga aacacttgag aaactcaccc acttttctgc aaatgcaaaa aaagacaatt
     4861 tcgatatctt gactttgatc tacgagttcg tccaggaaaa ttatgagaaa gaaatcaatt
     4921 tacaactttt atccgaaaaa tatcattttt catatagcta tctctcagcc atctttactg
     4981 aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt ccgtatcagt aaagccaagc
     5041 tctttttgac ggaaacacgg cttactttga cggaaatctg tgagaaagtc ggctataccg
     5101 aactgggtta tttctccaga gtttttaaag aagagacagg aatgacacct tctcaatacc
     5161 gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag catgaactct ttgccaaact
     5221 tctgttcatt ttgttcatcg gtctttttct gcaagccatc gtcatttctt tgtttattta
     5281 tcatcgatca agggatgcct atatccagct gttcaatcaa tcgaatgatg tggtactaaa
     5341 aaagattcaa tctgaatttg aaagtttgaa tgatacaatc gagaatacat tagccgcttt
     5401 tgattctaat ccagctgtca aaagctattt ttccaatgat ccagcccaac atatggaaca
     5461 gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct ctttcaaaaa tccatccaat
     5521 gatcgattac gatgtattga tctttggaga aaatggaaga acttttgtcg gaaacgacat
     5581 gctcactgcc gtatctgccg attctttttt tcaatctgcg attgctcaga gagtgaatga
     5641 acgtgctgct gatacacaaa tgctttttgc acaccacgga ttgactcttc gcgataagaa
     5701 agcacccagc gtcttctttg tcaaaaaact gaaaaataca ttgaatcacg tttacggata
     5761 tgctgtttta tcgatctcat ccaagcaact agcgaatctt tttcaatctg tagtaaatcc
     5821 tgagatatcg aagatctcct tcatgaacga agaacagacg atcatcgcat caaatgaaga
     5881 aaatacaatt gggagccgat caatgatttt caaaaaacta acagtaggtg aaacaacgat
     5941 ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc caggattgta cattactcag
     6001 tcaagtcgat cttcattctt tatctaacca aatgggcgtg atttttccga ttattttgta
     6061 taatatcgtg acacttgctt ttgctggctc attggtattt atgtatttgg atcgacacac
     6121 aaaatcgatc taccgcttga ttgattcgct aaaacggcta gaggaagatg gcttaaaagc
     6181 tactgttcct gtacaaggaa gccatgaagt ccaattgcta gggaatacga tcaatcaatt
     6241 gctaagtaat gctcagcatc attatgaaca gacgatccaa gacgaaaaga agaaacgttc
     6301 gcttgaaatc caaactatgc aagcacagat acagccccat tttatttata atacgctgac
     6361 tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa gcagttcaag gaatcgacag
     6421 tttcattact ttactaagaa gtacgattgg gaaaaaagaa gaagtgattc cagtcaagga
     6481 agaaatcaaa agcgtacaaa gttacatcga tcttttgagt atgcgatatg gcgaaagaat
     6541 ctcggctaaa ataatgattc ctgatgaact gcagtcatta tctatgccga atatgatcct
     6601 tcaaccaatt atcgaaaacg cctatcttca tgcttttcac aataaaacgc aaggattcat
     6661 cacggtgtac ggcaaaacaa aaaatcagct tcttttattc gaagtgattg ataacggcga
     6721 tggcttcgat actgaaaata ccaaacaaaa aaatgatcat ttttcaggaa tcggcatcag
     6781 ccatgtagat gagcggatac gcctaatgta tggcgatgca tatggcttaa cgatccaatc
     6841 agtgattggg acaggaacga ctgtaacgat ttgtctaccg cttcttccta aaaatcaatc
     6901 agaatatcaa aaataaagaa tagccaagcg ctgatttttt tattttaagt taaataaatc
     6961 aatcagtatt cttgactttc atctacgcgt ctcttctact gtcagcactg gataacagcc
     7021 caatatttga ctttctcctc cgagcatctt gatttcttgg agcgcattct ctactaatgt
     7081 cattggacgg tccaacaaca aatcgatcac gaaaaaatat tctcccaagc tagttttcaa
     7141 tggtcgggat tcaatcttac ttaagttgat ttcccgccag ccaaaagctg ccagcatttt
     7201 atgaagcatc cctggccgat tagcaggtag tgtcaagatg acactcattt ttactggagc
     7261 tgactgctgg ctggtcatct ttcggtcacc gacgatccaa aaacgtgtct ggttcaactc
     7321 attatcttgg atattctcag ctaagatctc caatccaaca tgctgtgctg tttctaatga
     7381 agcaatcgct gctgcgtctt ctttcggatg ttctgctaca tacattgctg cagcagttgt
     7441 tgattctgtt gctactaaag gaacattagg ataatgcgtt tctaaaaact gttgtgattg
     7501 tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt gttgctggat tgcctaaaag
     7561 ctgctgcttg ataggaagaa cgatttcact tttaacttcg acttctggat gtttggaaag
     7621 aagatcgatt gtgtgatgaa cagctccttc caatgaattc tctacaggaa cgacagccag
     7681 atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg atggaagcat acgcagtcaa
     7741 ttgttctgta ggaaatagct gacaagccgc ctgataagtg aatgaacttt ctggccctag
     7801 atagctgacg ttcatatgct tttgatcctt tctataatgg tatcgatgat cttttctggt
     7861 gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt cataccaaga aagtcgttgt
     7921 gccaataatg cttttatttc ttctcttgtt tttccaagag ctaaaggtcg aatagacgtt
     7981 tcatcttgtt ggatacgatc aactagacag tccgcttctg cctctaagta aagaacaatc
     8041 ggctgtgttt ttaaaaaacg ccgattttct gcactttgga caatccctcc accagtcgcg
     8101 ataattttgt cttcatttga cagctttctt aaaatatctg actccacttt tcgaaagctt
     8161 ttttcaccgt aatagtgaaa atattcggca atcgacaatt ggatatgttc ctcaatcttt
     8221 gtatctaaat ctacatactc tttttgtaaa cattgagcta accctttacc gattgttgtt
     8281 tttccagcgc ccatgaaacc aatcagcaaa atgctagcca tgagcttctc ctcctgacac
     8341 aagttctgct aaatcgtcaa aaaatgccgg ataagagatt gaaacagctt cagctccttc
     8401 tagttcgcaa ggtccatcag ctatcaaagc agctacttgc agcatcatac cgattcgatg
     8461 atcgcctctg ctggacactt ttgcgccatg cagaggtgtc ggcccatgga tgatcagacc
     8521 gtctgctgtc gcttttatct ttgctcccat tttctgtaat tcttctgcca ccgcatcaat
     8581 tctgttggtt tcttttactt ttaattcttc tgcgtctttg atgatcgtga ttccttctgc
     8641 ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc agacgcggaa tgatttctcc
     8701 accaatcgtt gctttcttca aatgactcgt cttgacactt aaatcagccg attggttctg
     8761 ttcattgtga ttcgtctggg taatcgctgc gcccatctcc tccaacacat ctaagatacc
     8821 agttcttgtt ggattgaccc cgacattttt taacaacagt tggctttccg gaacaagaag
     8881 tcctgctgct agaaagaaag cagctgatga aatatcacct ggaacagtta tctgttgacc
     8941 agttaatttt tgcggtccgg tcactatgat cgttttatct tccactgtga tccttccgcc
     9001 aaattgacga atcatttctt ccgtatggtt acgggaacgt tctttttcaa cgattacgga
     9061 tgtgccttct gcttgtaatg ccgcaaacag caaagcagat ttgacttgcg cactggctac
     9121 aggcatattg tatcggatag gtgataaaga gcgggttcct gtcacagtga ttggtagata
     9181 ctggtcattt tcttctcctt gcaagtcagc gcccatctcg cgtaaaggtt ccatcactcg
     9241 ccccattgga cgtttagaca acgaagcatc cccgaacaga gtagtcgtaa aaggctgtcc
     9301 agccaaaata cccatcaata aacgtatggt cgtaccggag tttcccatgt caattgattc
     9361 ggttggttgg acaaatgagt ggctaccgcg accatgaatc ttgatcacct cttcctcttc
     9421 ttcgatctcg acacctaatt gttggaaggc atgtaaagta ctcagacaat cttctgcgcg
     9481 caaaaaattt tgtatagtcg tcgttccttc agcaattgct ccaaacatga tgctgcgatg
     9541 ggaaattgat ttatctgctg gtatcctaac agtccctctt aatccatgta tttgttgtaa
     9601 taattgcatt tatccagcct cctatttgat ccagcaatga taactggtat tagacataat
     9661 gattcttttt gcttgtaaca aatcctgttg gtttttaaaa ctgatttgca gtattcctaa
     9721 aatatcttcc ctggtctctt ggatcttcaa attaatcaaa gaaactcccg cttttccgat
     9781 cagtccagtt acttctgcaa taacgccagg ttcatctggc acatcgataa ataaatcaaa
     9841 aaaggcagga atcgcgcctt gcttatggac aggcaactgg tctcgacttt cttttgcccg
     9901 attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg atccattctg ctatttgtgt
     9961 catttcttgt tgccaatctg ccaatcgttc caataatatc tgcttattgc tcaacaaaat
    10021 atctgtccac atgacaggat ctgaggaagc gatcctagtg atatctcgaa agcctcctgc
    10081 tgccagttgt ttggctcgcg gatactcttg attgaattgt tctgcctgat tgactaagcc
    10141 agctgccaga atatgtggaa aatgacttag cattcctgtt atacggtcat gttctcttgc
    10201 tgtaagagtt acatattttg ctttggttcc gcaaaatagt tcttgcagtt tttcaacatc
    10261 ttttttcgtc ttttccaaag gtgtaaagat gtaataggca ttttcaaata gtttagcatc
    10321 tgctgcctgt atacctgatt tatgagaacc agccattgga tgtccaccga caaaccgaag
    10381 atttttctgc tcagcggatg tcacgacttg ctgcttcgta ctgctgacat ccgtaatgat
    10441 gacgtgatca gacaagttga ttctttcaag gacatcaaga taagctaagg ttgtacggac
    10501 gggaacggct aataagataa cgtctgcttg ttctgcacct gtttcaaaat cacttgcaac
    10561 gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt tttgactgac tatcccaacc
    10621 aactactatc agttctggat tggtctgacg aatacaaagt gccatagaag caccaatcag
    10681 tccaagcccc acgataaaaa catgttgtgc catcttattt gccccctttt tagtaatgtt
    10741 tcaagtagtc acggtactcc ttcactgcag ctttcaactc aacaagcgaa tcacttggga
    10801 attttgacaa tagctctgtc gctaccacag tggctacaac attttccgca acgacgctag
    10861 ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc tttgtaaggt tcttttgtat
    10921 cgatatcaac actttgaagt ggcttgtaca atgtaggaat gggtttcatg accccgcgaa
    10981 tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc gcctagatta ttggtacgtc
    11041 ggaaatagcc tctttcttct tcccatagga tctcatccat cacttctgag cctggtaatg
    11101 aaccagaagt gaatccgacc ccaaattcta cgcctttaaa ggcattgata ctaaccacag
    11161 cttgtgcgat tttggcatct agcttcgtgt cccattgtac gtagcttcct aatccagctg
    11221 gaacgcctcc aacgagcact tctacaattc ctccgatcgt atctccattt ttctttgtct
    11281 gatcgatcaa atcttttatt ctttgctcaa ctgctggatc aacaactcgt acttctgacg
    11341 cttcagaaat ttctttgatt tcttgaatcg agatatttcc aggtatttct gcttggattc
    11401 caccaagaca aatcacgtgt cctgctacga tgatgtcaag ttcttccaga attttcttgg
    11461 caattgcgcc gattgctaca cgcatggtcg tctctcttgc agaggaccgt tcaagcacat
    11521 tccgaagatc tcgatgatgg tatttcatac cgcctaccaa atccgcgtgt cctggacgcg
    11581 gacgtgcaac tcgccgcagt gattggtctt tttcaggcac ttctgctaca gacatgacag
    11641 ttgtccaatt tttccaatct ttattttcta ccactaaagt gacaggtgaa ccaattgttt
    11701 tgccgtgacg gatgccagac gtgatccgaa cacgatcttt ttcgatcttc atccttcctc
    11761 ctctgccgta accagcttgt ctgcgagcga gttcgtgatt gatttcttct tctgacagag
    11821 gtaatcctgc tggtagtcct tcaataatgg ctgttaattc tggtccatgg gattctcccg
    11881 cagtcatata acgcatctta cagtcctcct aagtaatctt tgaatgtctc tgtgggaaca
    11941 ggaacgattt ttgcttgccc aatcttttct agcaagatga tattcaattg gcttcctctt
    12001 gctttcttat catgggttat cgcgtggaat aattcatctg gatgacttag atcgtacgta
    12061 atcggcagat ggaatttttg aagtatcctt tctaaagcag ctgtcgatcc agttggagac
    12121 aagccgattt tttctgcatg agaagtgatg gctaccattc caatagctac tccttctcca
    12181 tgactgacta ccccatatcc tgctgtattt tcaattgcat ggccaattgt atgaccaaaa
    12241 tttaaaataa gtcgatttcc ttggtcaaag acatcttctt ccactacttt tcgtttgatt
    12301 tccaacgtag cagtaatgat tggaacagca tgttccagaa gatctttttc gtctttcagc
    12361 tcgttcagct gtttccaaag cgttgcatct gctatagctg ctgatttgat gatttcagcg
    12421 attccttctc gtaaacgacg taccggcaaa gtttttagaa cctcaggatc aatcaataca
    12481 ccagctggct ggctaaatgt tccgacaagg tttttcgctt tgtttgtatt gactgctgtc
    12541 ttcccgccaa tactagaatc tacttgtgca agtagcgtag tcggaatttg aacgaaatgg
    12601 atcccacgca tgaacgtcga ggccacgaat gctgctagat cacccaccat tcctccacca
    12661 agtgcaagta tcccatcgct gcgggtaaaa tcattatctg ctaaaaaatc gtataactca
    12721 gctgcttgtt ctaaagattt actggtctca cccgccggta cgattgaaag gactggttca
    12781 aaattagctt gtctcaattg gaacatgatt ttttctgcat atagtggagc aacgtttgta
    12841 tcagtgatca cagctacttt ttgaggtctc caaatcgttt gaagccatga tccagcctgt
    12901 tctagtgcgc ctttttcaat atgaatatcg tatttttggt tatcaacatg tacagtaagc
    12961 attcgtttca cttctttctt ttaaaaagct ggaattaatc gagtgtctga atcttttcca
    13021 ttgcttcttt catgatcgca acttctttca tcatgcgtga gtaagtcttt tcattcaacg
    13081 attggggacc gtctgaccaa gcatttgcag gatcaggatg gatctctacg atcatgccat
    13141 ctgctccgct tgccacaccc gcacgagcca ttggtggtac aagttcccaa attcctgtac
    13201 catgacttgg atcaacaatc acagggaaat gagttaattt tttcatcaat gggacagcac
    13261 ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg aatcccacgt tcgataaaaa
    13321 tgacaggaga tttgtcttcg actgcaatgt attctgcagc attaagccat tcattgatcg
    13381 taccggaaat tccacgtttc aatccaacag gtttacctgt tttaccgaca gcagatagca
    13441 gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat atcgctgtac tcagccacca
    13501 tatcgatatg cccttcatcc atcacttcag taatcacttt cattccaaat tcatcagccg
    13561 cttgacggat gtatttcaat ccttcttctt ctaaaccttg gaaagcatat ggagaagtac
    13621 gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc ggctttggcc attcgcgcac
    13681 attctctgat ttggtctaat ccttcgatag aacaaggacc agccatcgtc acgaaactgc
    13741 cgtcaccaat ttttactccg tctacatcca cgactgtatt gccaggatgg aattctcgag
    13801 aagtcaattt gtatgtgttt aaaatacgca cagcattttc tactccttca tagcgtgtaa
    13861 atgctccttc ttgtaaactt cgcgtatcac cttttagtcc aatcactact cgttctgttc
    13921 cttgatcgat tgagactttg aatccttctt tttcgatatg atcgacgact ttttttattt
    13981 gttcttccgt tgcttctttt ttcataatta ctatcattgt ttttcccgct ttcttttgtt
    14041 tacttatcta tcttggctga ttttctctac tttttcaagg tggttttaca ttaattccta
    14101 ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc tttcctactt atgacccaga
    14161 taatcaacaa aaaaagatgt ccctgtagga aattgcctct acagtgaaca tcttcaacga
    14221 gccttaaaaa gcgctgcaaa aatataaaat agccactata gatgaatttc tctacgtggc
    14281 tgtaagtcgt gtttaaaaat ctttagccat catagataca aacctggttt gtttgtaccc
    14341 acgaagtcat gaatacaaac ctatctaaaa tagctaaagt aatacgtatt cagttttgta
    14401 agttttctag atttcataaa aaatcgctcc aaactgatgt aagaattgta cctagtatag
    14461 acataaaaaa atcgactgtc aatcattttt atgaaacttt ttttaatttt ctgatagtta
    14521 actttgtttt tcagaaaaat aacaattggt tttattgaag aaaatagtca tgaacaaaag
    14581 tatctgagaa cttttttact ctcttgttca tgacatatag aataaagcag ctttgtcttg
    14641 taaactccgt ttttcaaata ataatctaaa gattgctact gaataataaa gccgatttca
    14701 tggtcctctt cgctgctgat tcggtaaggt gactcaacat ccgctcccca gctattgata
    14761 cccccgactc ctcttacttt tgcaaaaatc gtaagaaccg ttcgtctttc tggcggtagt
    14821 tcttctatat gtgttgcctg ttccagttct tctgctttga atggtaaagc ggaaaaagca
    14881 aatggcgtat cctgctgttc aaatcgcaac gaaaaatctt cttgatcttt atccgtatta
    14941 tccaacgttt gatttcttgt tagttctacc catttcgttt ccatatgcat gccgcattct
    15001 tggggaacaa ggtatgggga aacggggagt cctgccactt tataaatacc ttcgacacca
    15061 ccagccatcc gatccggata tgtttcccct gataggcctt catactcaaa aaattttgct
    15121 ttcgttggca taatgaacct taaacccaat gcgggtaatt ccggtaatcc tttttgtcca
    15181 taataatgca tattgacaag cattttaccc tctccagcga tttggtagga aattgtcact
    15241 tgggtttttg gtactgtgtt gatttccagt gtaaaaatga tttcaaccgt ttcagcgtat
    15301 tcattacttg aatattgatt atttactgga gcaatcggca aatcgatctt tttattgtcc
    15361 accagtactt ctatttcttt gacttttgga tacaatccgg ctcctaacca cataccggat
    15421 ttaaatccaa atccgctacc tctgtcatta tcggtcgttg ctcgccaaaa aactggaagt
    15481 gtttcccggt atagccattc ttttccgttt ttattgagcg attccagtcc gcctcttgta
    15541 taagaaaaaa tataatgcgt attccccgat ttgacaccta atgtacagtc gccgaagacg
    15601 attgcaactt tttccgtatc catatgactt ttatttactg tatttttcat aataatatct
    15661 tacctcctgc atggcgggtt tttcttgtcc atctgcaaaa acgatcccat tccctgagaa
    15721 ttcatagtca cttggtttat cgtcaaagtc accgccatag cgcatcactt ctctgcctgt
    15781 gattggatca gtcacttgga tcgcctgatc tttaaaatcc catatatagc ctccttggta
    15841 catttcataa cgatcaagca acgtcatgta agaatccatt ccacctaacg aattccccat
    15901 gtcatgcata tattcacata agatgaacgg tttttccgct ttattttcaa ggtagtcaat
    15961 gatctcttct ggtggcgcat acatgcagct ttttacatct gaaatcgaat cttcgaatgc
    16021 tggatttgga aaattccctt cataatggac aagtcgatcg gggtctactt ttttgaagta
    16081 atcgttcatt tgtcgaatgt tttcttccgc ataagattca tttccaagag accaaaaaag
    16141 aatacttgga tggtttttta atgtttcgaa attcgttttt gctcgatcaa gaacagccgc
    16201 ctgccactga ggtactgcac ctggtacgtt gtaagaaggc tcaaatgcgc ccattttttg
    16261 ccatgagcca tgggattcga ggttcgtttc cgccatcaca tggattcctg cttggtcgca
    16321 taaataatac caagtgatct ggttggggta atgagatgtc cgtacgccgt tgatctttgc
    16381 attttttatg atagccaaat ctttttccat atcttctata gtgatggcgc gtcctttttt
    16441 cggatgccac tcatgtcgat ttaccccgtt gataatcatc cgttttccat tgaagtagat
    16501 gattttatct ttgatttcga ttttacgaaa accgaaggaa tagtcaatat actccgttac
    16561 tcttttttct tcgtctatcg tctccaattc aagcttatat aaattgggct gaaaatgccc
    16621 ccacagtgcc acattttcaa atccatcgat gcgaatcgtc atttgctctt ttaacggata
    16681 ttgctgttga tagatttcct tcccatcagg atctagtatc gctacattca gctgactgtt
    16741 tttggctact tgctcaaaag acaaatctac ttcaagtttg ccattttgac atttgtcatc
    16801 taagcgaggt ttgatccaaa gatcttctaa atggttttgt cctcgagcaa ctaagaagac
    16861 atctctgaag atcccaaaaa atcggaaaaa gtcctgatct tccaagaatg cagctgtact
    16921 tcttttgtaa acctccacag ctagaatatt ttcttcttcc aaataaggtg ttaaatcaaa
    16981 ttcagacgga gtgaacgaat cttcggcata cccgataaaa tgaccattca accaaataaa
    17041 cattgcttgt tctacaccag caaaatgaat cgagatttgt tttcccttta gaccttcttc
    17101 taatcgaaaa gtcgtcaaat acgaaccaac cggattgtct ttagcagctg aaaagatccc
    17161 ttccgcctct ttctcagggc ttgtcgtgaa agggggacgg cgaaatacat gtccctccca
    17221 cggatacatc gtattgatgt aatggatctt gtcataattt gctaattcaa tatgactagg
    17281 aacagtgatt tcatcaaaag aacaggcatc aaaatctttt ttataaaatg tttttggtcg
    17341 taccgaagga tttggcgaat aacaaaaacg ccatgatcca tttaaagact gtttaaggga
    17401 ggatgtggtt tctacttctt cccttgttgc ataacaaaca tgatcgctat gtgcatttga
    17461 ttggttcact ctaaaaactt gtggatcatc taaccatttt atatttgctt tcatactttt
    17521 ttccttctct ctcataactt tttgaaatga agcagttctg tttggaaatc accttgaaaa
    17581 tcggggaagt aaagtccaac tgtcatcaat tcggcccccg aaaatatccg cttcgaggtc
    17641 tgttcctgat aaagtgcctc atcatctaac cccatcagat atacttcatg gaacgctggt
    17701 tgagctgaag ctagtatcct ccctaaaaac acaatagctt catcttgttt aggagacaca
    17761 aacatccatg cggcaatatt ttcttcaaaa ggactcgcca aacgataata atggccatac
    17821 tggatcaccg gacgaatcgt ttgataggat ataatttggt cttttacttg ctttttttct
    17881 tcaggagaca gctttgtcaa atccagttca taaccaaaaa cagcgctcat agctgtttct
    17941 cctcttgttt tcaacgaagt agatctgtcg gtctgatgat ttggtacagc tgatacatga
    18001 gctgtgatgg acgaaatcgg ataagcaagg cttgttccat attggatttt catacgctcc
    18061 actgcatccg tgttatcact tgtccatgac tgcggcatat aataaataaa cccggcatca
    18121 aaccgtccac ctccaccaga acagccttcc cataagattt ccggataatc agttgtcaaa
    18181 tgttcaagta gttcataaag cccgagcata taacgatgcg ctacttctcc ttgtctctgt
    18241 gggtcaaggg taatcgaata gacatctgaa agcgaacggt tcatatccca tttgatgtag
    18301 tcaagaggaa tcgtatctaa aatctttctc atttgtttct caatcgtctg ccgaacatct
    18361 aaccttgtaa aatccagtac atgttgtgat ctggaagcag aaggcattct acctggctcc
    18421 tgcataagaa aatcaggatg tgttcgatac agttcagaat caaccgagat catttcaggt
    18481 tcaaaccata aaccaaattt caatcctttg ctatgagcat aatctgctat ttcacgtaat
    18541 ccatttgtca acttgccttc atactcgaac cagtctccta atgaagaatt gtccgcatct
    18601 ctttttccaa accagccatc atctaatacg aacatttcga tgcctagctc ctttgcttca
    18661 tcaatgatcg ccttgatttt ttcactattg aagtcaaagt aagtggcttc ccagttattg
    18721 accagaatcg gccgttcagc aaattgatga cgacctcttg cgattctttc acgtagcaac
    18781 tcatggtgga tctgactcat tttgttcaat ccctgacttg agtaagaaag gatcatttca
    18841 ggtgtttgaa aagactggcc tgcattcaat tcccacgaaa aacgatggct gttgatccca
    18901 ccgacgactc tcaattgatt gatctgatct ttttcaagtt cgaaagaatg gttgccggaa
    18961 taaacaaatt gaagtccgat cgcttctcca gtattttctg tcgtatgatg atgaactaaa
    19021 gcaataaagt tattcatatg gtgactgctc gttcctcgac ggctttcaaa tacttttgtt
    19081 ccagataaaa cagattgccg tgaaatctgt ctttcattga catgtgctcc tggcaaagca
    19141 atgacttcat caaatcgcct agtatgggca aagtctagct ggaaactagc cgctttttct
    19201 agtttgattg tttctccggt ttcatttctt atctttacag atcgtgtgat cactgctcta
    19261 tcagtaaata tggtatagaa caaatcgaaa taaatcgctg tttctctatc cttaagtgaa
    19321 atcaccaacg tttgtgcctc tgacttgtcc atcacataag tttgaggcaa gccttcgatc
    19381 tctggcttcc catctgtaat ataatggctg tcatatctca gatccagtaa gtctgatcca
    19441 ttcttgcgct gaatgatcga tgcgggcgtt cgaaagtcca tgctgccatg agaagagtat
    19501 tcttgcggta gtgtatcttt cgacagagac cggtcaggat ttaagggaac atttcctgaa
    19561 aatccacgat ctcttcgggg ataattttta ttgtctttgt actgcttgac tggtttgcca
    19621 aaataaatat gcgccaatac attgctttct tctatcttta tcagataaga aagaaatgta
    19681 tttcgtaaat ggaagactcg gctcttttca tcaaataaga tgaaattttt atctgttttt
    19741 ctatgcgtaa tatccatcat ttcactccca acaaaacgga aaccgtttac ttttttttat
    19801 aattcctgta tactgtttac taaacaattt tgtcaataaa aattttttat ttttttgagg
    19861 agtgtgtaaa aatggttgga attagagatg tcgcacgaaa ggcaaatgtc tctcctgcta
    19921 cagtttcccg tgtgttgaat aacgatcaga cgatccatgt ggctcagact actcggcaac
    19981 ggatagtaga ggcagctgct gcgttggatt atgacatcag tactcgtaaa tatatcaaaa
    20041 agagaatgcc ttcgattggt gtgatctcta cgatcggcaa agcgagcgaa gaacaggata
    20101 tctattacaa acatttgcga gtagggatgg aagaagaagc tcgacgtctg catttaggga
    20161 tgaatcgaat ctataatcta tctgataatc ctaaacaatg ggaagatcta gatcagttag
    20221 gtgcggtgat cgttgttggg actgttacga aagaatcgat taccgccttg ttaaaacaaa
    20281 acatgcaaat agtcgtcgtg gataaccctg acatccagca agaggtcgat atggtgtatg
    20341 cagatcttga acggatgact aaaaccgtac tggaattgtt cttagaaaat ggtcataaaa
    20401 gaatagccta tatcggggga tataaagtcg atatggatga attcggtggg aagcagattt
    20461 ctataaatga aaagaggatg cgtgcctatc agcattttat gatcgatcat cacttagaaa
    20521 aatatatgtc ttatcatcta ggtggctgga cagaaaaaca aggtgaggta cttgcgacta
    20581 atctattaga aaatgaatcc cctcttccta ctgccattct tgtcgggagc gatccaatgg
    20641 caattggtgt ctatcgggca ttgaaacaaa aaaatatcca aattggagaa gagattgttg
    20701 tagcaagctt tgataatatc gagacaatcg caaacctctc ccctggcttg accagtgttc
    20761 aaatcaatgc gaaatcaatt gggcaagcag ctgttcgaat ggcattggag cgaattgacg
    20821 gtatacggga cgaaccaatc attttgactt atcctgcacg attgattgtt agggaaagtt
    20881 ttatcccaag aacaaaaagg tattgaaaga gaggttgtga aaagccgctt cgtttcttca
    20941 tactcaaaac gaaacggctt tttcacaacc tcttacttat acttattcac aaaatccaat
    21001 gtctgctggt aacaattttg cgattctttt agcgtgtaat caaattggta ttcatgggta
    21061 atctcttttt ttgtatctgt ataaaacaag gattgcacgg gtacctttaa ttctgtcaac
    21121 cgattttcta atgcttgacc ttgctcctgg aatgagtatg catttccgtc agttatatac
    21181 gttggcggaa attccttaga aacatggtca gccacactag cttctttcaa ttgctcactc
    21241 gttttccaat cttttgttcc aatcaacgac cacgcaacgg ttttgacgaa aaacttcata
    21301 aatcgatcat ccgaatgctg ctgtgctgtt agcttcaaat caacaggtcc gcaataagaa
    21361 atggttcctt tcagactatc ttttggaagt agctgtttga ttttcatttg tttagcatac
    21421 gcatcattcg tttgtatagc tgcatattgc aatgcgattt gtgaacctgc actatctcca
    21481 ccaaaaaata attgttccat gtctagcaat ttgtcgtctt tttcttcagc taataaatct
    21541 ttgatcagtt cattcacttg aagaacttga ttagggtatt ctgaatcagg cgctaattca
    21601 taattcatcg caaccactgc gatgttggcg tctgaaacta attttgtcgc aaattctttg
    21661 acacccgatt tatctccgcc gacgaatcct ccaccatgta cccaaaatag aacaggaaca
    21721 ggccccttgc tgttttttgg atagtagatg tcataagtat tctctttata cttggactca
    21781 tacgtcaaat ctgttttttt cgccacgttt ttttctgctt gatcgaacgt ttttttatca
    21841 gtgatttgaa cagtatttga aaacatatgg ttgatcaaga atgctccagg tctgggggaa
    21901 agctggaagg caagaaagat tgcaaacgcg atcccaagaa tgattccgaa gaaccatccg
    21961 ataaatttaa gaattttctt tttcataagg ctccctcagt agttagtatt aagcattctg
    22021 attataccat agtgttattt cctatcaagc tctcctagat gatttaatga ttttccccaa
    22081 acttcttctg caatgctttt gacaaaagaa attttttcca ttgttcttca aacaaaacat
    22141 tttcttcttc tgttgaagca aatccacatt gaggacttaa gcacaaacga tccaaaggca
    22201 gatatcgtgt cgcttcgtag atccggtcaa tcacttgctg gtgctcttcc atttcgccat
    22261 ttttcgttac agtcaaaatc taagacaact aatttatctt cagagacttt ctctaaaaga
    22321 acaaaactcc cagcacgttc actatcgtat tctaaataat aggcacggac attttcttga
    22381 gtaaaataat gggtctgcta cgtcgtcatc ccccctgatg ccgccaaaat cgaataataa
    22441 tttccgcgtc aaacatgtat atttagtgtt gaatcatttg agaatatgaa aataaaaatc
    22501 tttttgatca atcagttaat caaattgaaa taacgaaaaa tctgagacag aatgacgttc
    22561 attctgaccc agatttgatt ttgcacaact caacagtcct tattggcaaa tcgccaaaat
    22621 aatgtagact aactaaaaag actaagaaac aataacaaaa gttaacactt cttagtcttt
    22681 ctttagcacg tctatttatt aaagatttta gggccttttc tcatgttttt acgatcattc
    22741 ttaggtgttt ccatttgttt ggatcggcct ccaccttgtt tctttttgat caattctttc
    22801 attttttctt tttggtccat ttttattctc ctttaacgaa tgaagcgact aattcgatat
    22861 gatgggtttg cgggaaaaga tctacaggct gaactttctt cacggcataa cctgcttctg
    22921 caaataactt agcatctctt gcaaaagttg ccggattaca tgagatatag acgattcgtt
    22981 ctggtgacat ttcgattgct gattcaataa aatgatgatc taatcctttt cgtggagggt
    23041 caactacgat cacagatggt ttgatccctt tttcttgcca ttttttcata actttttctg
    23101 cttttccgac ttcataatga acattttcga ttttattatt taaagcattg aactgtgcat
    23161 cttcaattgc ttctggaata acttccatcc catacacctt atccactctg tctgctaaag
    23221 acaagccaat cgtacctatt cctgaataag cgtcgatcac aatgtctttt ttatctaact
    23281 cggcaaactc gatggctgtt tggtacagat actctgcttg ttgagtattt acttgataaa
    23341 atgactttgc tgagattcga taggtttttc ctaatagctg atcttcgata tagtctctgc
    23401 catataaaac tttttctttg tctcctaaaa tcacatttgt tttttcttca ttgatatttt
    23461 ggatgaccga aaccacttct ggcaattctt catgaatagc ttcagcaatt tccttggatt
    23521 taaagaattt ttctttacga gtaactaaaa cgaccatcat ttcatgggaa taatgaccac
    23581 gacggataat gatgtgacga agctgtcctt catttttttc ttcattatat cctcgtactt
    23641 ggaaccgttg caagatatct ctgactactt tgattgcttg atcgatgaca ggatcttgga
    23701 tataaaaatc ttcgataggg actagatcat ggctattctt tttgtaaaaa ccggtggcta
    23761 actgtccatc gatacggcga acagggatct gtgctttatt acggtagccg aatggttctg
    23821 ccattccgac cgttggaagt acaggaactt ctggcatctt agcaatcttc gccattacat
    23881 gctctacttg tttttgtttg aagacaagct gttgttcata cttcaaatgg gctaaaggtg
    23941 cgatacctat gcgaagtaat gcattgtttt ctacaggctg acgatcaggt gattgtgtaa
    24001 taaatgtttc gacttttcca tatccaaact tattgcctgc ttttactact agtatttcta
    24061 cttcttctcc agggagtgca ttttcgataa ataatggata accatcgatt tttgctaccc
    24121 ccattccttc ataactcaag tcgacgattt cgactgtata tcgttcgttt ttctttacta
    24181 tttgctgttc tgtcatattt tgctccttta agcctatttt tcaatcctac tgaactctac
    24241 tataaatcag ctaattacac aactaaaacc ggcactgacc ttactacgtt cagcaccggc
    24301 ttttgattaa ttcttttgat taggtgtttc gtctgctatt tttccgtcac cgtcgatatc
    24361 ttcatccgtt aaacgttcga cttcttttac gaattctttg ctgacaacct ctaattcttc
    24421 ttcatctgta cctgtgattg cttcatcagg aatctgatcg gtattggcaa agaactcaat
    24481 gtgttgatga agattttgga aatgcatcgg tgcttccccg ccgtattcac catctaagtt
    24541 gatcattact ggtcgattga tatcatcttc tgtttcaacg aacaaactgc ttgttttggt
    24601 atagattatt cttggatcat cgacatgtcg accgccattc agcatgagtg ctgcaatatg
    24661 caagatctca aagatatttg ctgtcttcac aatgattaaa gaaaacttcc catcatccaa
    24721 tttggcatct ggcgcgatct gttcgaatcc tccaacagag ttcgtcagcc ctaggaaaaa
    24781 catcgaagca tttccgatat attccccttc atcatacttc aagcgcattt tgattggctt
    24841 tactcgaggg agcatctctg ctcctttcgc aagataagca agataaccaa aaatactctt
    24901 tagttctgat ggaacttcat aagtcaattc tgtcaaatag ccacctgcag cgatattgat
    24961 gaagtaactg ttatcagaaa ggccgatgtc catttttacg gtttggtgtt tttttatcac
    25021 ttctgctgct gctttaatat tatctctagg aatttttaat gctcgagcat agtcgttcgt
    25081 tgtaccagct gggatgatgg ccattttagg acggtgttca agcggagcga ttccattaac
    25141 cacttcgttg attgtgccat cgccaccagc tgccactact agatcaaaac ctagttctgc
    25201 gactctacat gcttcattct tggctgaatc tggttctggt gtagttgcat aggcgctggc
    25261 ttcaaatcct gcttcttcta atgcctgtag gatatccgcc aaattttttt tcaataattc
    25321 ttttcccgag gtggggttat aaattacgcg tgcttttttc attttcagcc tctatcgttt
    25381 cgctaattca tctttaagta gcttattcac gacaccagga tttgcttgtc ctttagttgc
    25441 tttcatgatt tgaccgacta ggaagccaac cgcacgatct tttccatttt tgaaatcatc
    25501 gacagattgt tggttgttat ccaaaatttc attaatgatt ggcagtagtt tgttcggatc
    25561 agataattgc acccaaccat ttttttcaac tacttcttgg gcttctccgc catttgtgat
    25621 caattcacgg aagacttttt tggcgatttt tgagctgatc gttccatcgg cgatcaaacg
    25681 aatcattcct gctaagttct caggtgtcaa ttttgtctca gctaattcca gtttctcact
    25741 gttcaagtaa gcagaaactt cacccatcaa ccagttagat gcttgcttgg catctgcacc
    25801 attttccaaa acagcttcaa aaaaatcaga catttctttt gtaagtgtca ataccattgc
    25861 gtcatactct ggaagtccaa actcacggat atagcgttcg cgacgagaag ctggcatctc
    25921 aggcaaacta gcttttactt tttgaatcca ttcgtcatcg atcacgaatt tcgggatatc
    25981 aggctctggg aagtaacgat aatcactaga cccttctttg acacgcatca aaactgtttt
    26041 attcgttgtt tcgtcaaaac ggcgtgtttc ttgttggatt tctcctcctg aaagaagaac
    26101 ttttgcttga cgtttttctt caaaagctaa accttttttc acgaaagata aagagttcaa
    26161 gtttttcaat tctgctttcg taccgaactc ttcttgtcca taagggcgaa gagaaatatt
    26221 ggcatcacag cgcatagagc cttcttccat tttcacatcg ctgacttctg taaattggat
    26281 aattgatcgc aatgcttcta gataagcgta tgcttcttct ggtgaacgca tatcagcttc
    26341 agaaacgatt tcgatcaaag gcgtgccttg tcggttcaaa tcaacgtaag aataaccgcc
    26401 aataccatgc atgttttttc cagcatcttc ttctaaatgg acacgttcga tccggatttt
    26461 tttcttttta ccttctactt cgatttcgat ccatccgtca tgaccgattg gttgatcaaa
    26521 ttgggaaatt tgatatgctt ttggattatc tgggtaaaag taatttttcc ggtcaaaatg
    26581 agtatcttta gagatttcac agtttaatgc caaagctgct ttcatcccga attccagcgc
    26641 agctttattc attactggca gcacgcctgg ataaccccag tcgatcacgt ttgtattgct
    26701 gtttggttct gcaccaaaat gagctggcgc tggagagaaa atttttgagt tggtttttaa
    26761 ctcgacgtgg acttctagtc caatgactgt ttcaaaattc attcgtcttt ccctcctaaa
    26821 atagctggtt gttgtttatg gaattctgtt gcttgttcaa acgcatatgc cgcttgatac
    26881 atcgtttttt catcaaaata attaccgatg atctgcaatc caactggtaa tccttctgat
    26941 aaacctgccg gtacagacat acctggaagt ccagccaagt tgacagggat cgttaagatg
    27001 tcactcatgt acattgtgat tggatcgtta atgttctcac cgattccgaa tgcaactgtt
    27061 ggtgaagatg gcccgatgat caagtcataa tctgcaaaga ctttttcaaa atctcgtttg
    27121 atcaatgtac ggacttgtcc tgcttttttg aagtaagcgt cataataacc cgcgcttaag
    27181 gagaatgtac ctaacatgat acggcgtttt acttcttcac cgaatccttc tgaacgtgag
    27241 ttgacatata catcatctaa tgatttcaca ttttcagagc gataaccgta gcggatacca
    27301 tcgaatcgtt gcaaattgga acttgcttct gatgaggcaa tgatataata aacagccaca
    27361 ccgtattttg aatgaggcaa gctgacttct tccacaacag cacctaatga acggaatgtt
    27421 tctgctgctt tcaatacagc ttcttttacg ccaggggcca caccttcgcc taagtattct
    27481 ttaggtaaag caatcttcat tcctttgatg tcgcctgtca gtccctctgc aaaatcagga
    27541 actgaaacgc ctgaagaagt accgtctttt tcgtcataac cgctgatagc tgtcaatgct
    27601 aatgcattat ctttcaccgt acgggtcatt ggtccgattt ggtcaagaga tgaagcaaag
    27661 gcaatcaaac caaaacgtga gacacggcca taagtaggtt tcatccctac gatgccgtta
    27721 aaagctgctg gttggcggat acttccccca gtatcactac ctaaagaaac tggaatttgt
    27781 ccagctgcaa ctgctgctgc agaaccacca gaagagccac caggtacttt tgttgggtcc
    27841 caagcatttt ttgttttctt gaaataagaa gtttctgtac ttccacccat agcaaattca
    27901 tccatgttta gcttaccaac agggatcata tctgcttgat agattttatc catgaccgtt
    27961 gcatcataaa ttggttcaaa attgtaaagg atcttagatg cagcggttgt caagatatct
    28021 tttgttacga tattatcctt gataccaatt ggaataccag caaggacgtt gctttcatca
    28081 atcccttttt catctaaagc ttttgcctgt tccaatgctt tttcttcgtt caatgtaatg
    28141 aatgcctcga cctcaggctc tgtttctttg attcgttcaa atgttgtttt tgtcaaatct
    28201 tgtacagtga tttctttcga tacaagcata tgatgcagtt cttctagaga acgattgtgt
    28261 aattctgtca ttatgcgcca gcctccccat tatcgatgat tgcgggtact ttgataaagc
    28321 cgtcttctgt ttccggcaca tttttcaata attcatcgcg gttccatcca gcttgcgccc
    28381 gatcttgtcg catcacgttt actgtttcaa ccacattcga cgtaaatgga acaccttccg
    28441 tatctacttc ttccagcatt tcaaccatat cgatgatttt acctaattgg tcggtaaaac
    28501 ttgcaagttc ttcatctgcg aacgcaagtt ttgctaattt ggcaacgtgt ttcacttgtt
    28561 cttcactaat cgccatacta ccccttcttt ctaaacgagt taaaaacttt gctctctatt
    28621 ttaacacaag agtgccgtgt ctgaaattgt ttattgaaat ttgtctgaaa aaactcattt
    28681 ttcattcttc tactgagcga cttttagcca atgcatctgc catttgatct tctgtccaaa
    28741 cttcgattcc taattcttgt gctttggtca gcttgctgcc agcatcttct cctgctacca
    28801 cgatatccgt ttttttcgat acgctaccag tcactttccc gccaagattt tcgatcgtct
    28861 cttttgcttc ttcacgagta aatcgtgtta gtttaccagt aaggactact gttttttctt
    28921 taaaaggaga ctcgacttct tgcaattggg catttcgaag ccctttgtat tcgaaattga
    28981 cgcctgcttg ttttaattca ttcatcaatt catggacttc ttcgttactg aaataagtga
    29041 ctacactgtc ggcaatgata tcaccgatcg tatcaagcgc aataatcgac tcatagtcac
    29101 ttttactcaa tgtttctaga tcaccgaaat gttctgctaa aatttttgct gctttcgctc
    29161 cgacatgtcg gattcctaac ccgaaaatca accgctcgac cgaattatct ttgctgttgt
    29221 cgattgctgt caaaatatta tttgctgatt tttcttttat tttgtccaac gtcaataact
    29281 cttcttctgt caatttgtaa agatcagcaa catctgctac tagtttttta tcatacatct
    29341 gttctagtac gcgcggcccc aacccatcga tattcatcgc atttcttgaa acaaagtggt
    29401 tcaaaccttc tttcatttga gcagggcatt taggattgat gcaccgtaag gctacttctt
    29461 catccaaatg gacaagttca cttccacaaa ctggacaatg tgtaggcaat tggtattctt
    29521 cgctgtcttt aggtcgttta tctaacacga cttgcgaaac ttctgggatg atgtctcccg
    29581 ctttataaat cagaactgta tctttcaagc ggatatcctt caacttaata taatcaccgt
    29641 tatgcaggct ggcacggctt actgtcgttc ctgctactcg gactggtgtc atgatagctg
    29701 ttggcgttac tacccctgtt cgaccgactg tccattcgat gttttcgatt agcgtttcaa
    29761 cttcttctgg cggaaacttg taagctgctg cccatctcgg tgctttgata gtaaaaccaa
    29821 gttgatcttg tagcgagaat tcattcactt tgatgacgat tccatcgatt tcatagggca
    29881 aatccacacg tttgtcatga tattcttcaa tgtatgacca cacttcatca atcgaatgac
    29941 atagacgttt ttcccgattc gtgtggaatc caattttttc tagctcttct agcgcatcat
    30001 actgggtttt tgcttgcata ggaccgaaat cagctactgt gtataaaaaa gtgtccagat
    30061 ttcttttcgc tgtgatttta gaatctaact gacgcaaaga accagctgcg gcgttccgag
    30121 gattcgcaaa gatatctttt ccctctgctt ctctgtcttg gttcaattgg acaaatgacc
    30181 gttttggcat gaaacattcg cctcttactt cgatgttcat tggttctttt aatttgattg
    30241 ggaccgaccg gacagttttt aggttttcag tgatgttttc tcctacagtt ccatccccgc
    30301 gagtcgcacc tctcacgaaa tttccatctt cataacgcaa agaaacggaa agtccatcta
    30361 tttttagttc acagcaatag tcaactacac gtcccactgc tttttgtaca cgttgatcaa
    30421 aagctatcag ctcttctttg ctgaaaacgt cgttcaaact gtaaagcgga atatcatgag
    30481 tgactttttc aaaaccttct aatacttttc cgcctactcg ttgagtggga gaatcagacg
    30541 tgatcaaatc aggatattgt tcctcgattg ctactaattc tgcgtattct ttatcataaa
    30601 catagtcttc cactgttggt ttatcttcta cgtagtattc gcgagaccat tggttcagtc
    30661 ttgtacgaag ttcttctgct cgtttagcag cttcgtcaat cgtcatttca tgttccatca
    30721 tttaccctcc actactaagc tttttcaatt ggcgcaaagg cagccaaaag acgttttact
    30781 ccttgacttg ggaaagccac atctagttct agatccttcg acgtcccgct tactcggacg
    30841 actgttccta gaccccattt tttgtgtttg actttatctc caggtttcca gctgttattt
    30901 tcccctccag tagcactttt agagggaaca accgctgtct ctggctgttt atagctatat
    30961 gctgtttttt tagcgaatac aggcgttttt ttaggcgtag gacgcatgcc ttcgatttcc
    31021 agtaactcct gatcgatttc ttctacaaaa cgagaaggtc ggttatattg cgtccgtcca
    31081 tataatgttc tggagaaagc atttgttaaa tacaacgctt cttctgctcg cgtgatcccc
    31141 acatatgcga gacggcgttc ttcctccagt tcgctttctt ccatcaatgc tcgagataaa
    31201 ggaaaaaccc cctcttctag accaatcagg aatacaactg gaaattctaa tcctttggct
    31261 gcatgcaaag tcatcaatgt cacttgggaa gcatcttctt ctaaattgtc aatgtcggaa
    31321 acaagtgcca gatcatttaa gaagaccgtt agtttttctt ctggtgcatc agcgtcttct
    31381 tcattttgct gttcgaattg tttgtcgaat tcttgtgtga ccgttaaaaa ttcctccaag
    31441 ttttcgatgc gtgcttgtgc ttctaatgta ttttggattt ttaagtcttc tagatatccg
    31501 ctacgatcca aaacctcttt cgtcaattct gtcactgtca agtatgggat catctgagtg
    31561 acttcttgga tcatctctcc aaatgcacct aattgctgtc ctgcttttcc accgatattt
    31621 gccagatcca cattttgcgc tgcttcaagc agtggccatt catgaagaga agcaaaactg
    31681 cgtagttttt cgatagatcc tggtccgatg cctcgtttgg gcgaattaac gatacgctca
    31741 aaactgattg aatcttgagg atttaccagc acattcaaat acgctaaaat atctttgatt
    31801 tctttacgat cgtagaactt gtgccctcca accattttat aaggaatatt tgctttcaac
    31861 agagtttctt ccattacacg agattgtgcg ttcgtccggt acaagatggc aaagtcgccg
    31921 taattccgat gattgcttcg aatctcttct tgcatccggt ctacgataaa acgggtttcg
    31981 tctcgttcat tatctgcacg gtagtaagtg atcttgtttc cttctttgtt ttctgtccac
    32041 aagttttttg gttttcgatt gctattgttt tcaatgactt ggtttgctgc gctaaggatg
    32101 tttttcgtgg agcgatagtt ttgttctagc aaaattactg cagcatctgg ataatctttt
    32161 tcaaaatcca agatgttctg catatctgca ccgcgccagc cgtaaatact ttgatctgca
    32221 tctcccacga cgcacaggtt cctaaatcta cctgccaata aattgactag cgtatactgt
    32281 gcgtgatttg tatcttggta ctcatctaca tgaatataat ggaatttgtt ttgataatac
    32341 gtgagtgaat caggatgttc ttcaaataat cgaatcgtat tcatgatcag gtcgtcaaag
    32401 tccatacatt gattatattg aagctctttt tggtaagcag cataacattt tgccgcaatc
    32461 tcttcgtaat aactgccttg catttttgca aaatcctgag gggtctgcag gctgtttttt
    32521 gcttgagaga ttgttcctaa tatacttcga ggatcatatt ttttcggatc aatgttcagt
    32581 tcttttagta tccgtttcat caaggtcagc tgttcagaag aatcgatgat tgtaaagttc
    32641 cggttatacc cgataaaatc aacatctctt cttaaaatac gcacacacat cgagtgaaaa
    32701 gtcgataccc agacatcttc gcctccagat gcgagaatcg cattgacacg ttctttcatt
    32761 tcttttgcag ccttattggt aaaagtaatc gctaggatgt tccaaggatt gacttctttt
    32821 tcctcaatca aataggcaat gcggtgtgtc agcactctcg tctttccact tcctgcacct
    32881 gccattagca gtaatggtcc gtcagtgtga agaacagctt ctttctgacg ggggttcatc
    32941 ccgtttaata attcagcttt ggatgacatt aacaacatcc tttcttttat caatcttgtt
    33001 aattatagca ttttcttggc tttcattaaa ctagctagtt caaaatcatt tgtttctttt
    33061 tatggatttt tccttgtcaa ttcgttgatt tcgcgctaaa ttaaaagaaa gcgaggcgtt
    33121 tgaatggaaa aaaaaggaaa aatcaagaaa ttagataaga ctcagatata ttttattcta
    33181 aaaggaatat ttgttggact gctgacagga atcgtcgtta gcctgtttcg attatctatc
    33241 gaaaagctaa gcgatttagt tcgaagtatc tatgaaatga gtcgggaaca gccgatttac
    33301 ttaattggcc tagctatttg ctgcatcctt gcagcatttt tcgtgggtta tctggtcaaa
    33361 aatgaaccgg atatcaaggg aagcgggatt ccacaagtag agggacagct tcgcggggaa
    33421 ttatcaatga actggttctc tgttttatgg aaaaagttca ttggcggtgt actttcagtt
    33481 ggtgcgggcc tttttctagg tagagaagga ccttctatac agcttggcgc atccattgga
    33541 caaggagctg gacaattgtt tcggagtcca tcttcagaag aaaagatttt aatttctagc
    33601 ggtgcaagta gcggacttgc agcagcattc aatgctccta tcgcaggatt actctttgtt
    33661 ttagaagaaa tccatcactc cttttctcca cttgtttggc tgacatcatt tgcttcagca
    33721 atcactgcta acttcgtctc tctttacttt ttcggcttga gacctgtttt atatctaggt
    33781 gaccttcctt ctttgccgtt gaaatattat ggttccttag tgatcttagg agtacttcta
    33841 ggcatcttag gttttattta tcagaatgtc ttgctggctt tgccaaaatt ttacaaccga
    33901 ttgcctttac cagcttattt tcacgggttc gttcctttct tgctgatttt gccaattggt
    33961 tatttatggc ctagtactct aggcggaggc aatcaaatcg ttttagcttt tggacatgct
    34021 tccttgccaa tcattatgat tatcggtttg tttgttcttc gattcgtttt ttccatgatt
    34081 tcttacggtg ggggtcttcc aggggggatt ttccttccga tattgacatt gggggcttta
    34141 atcggtagct tttatggaaa cattatggtt gatttcggga tggatccaat ctacatcaag
    34201 gattttgtcg ttattgccat ggctggttat ttcacagcga ttggaaaagc accgctgaca
    34261 gcaattattt tagtgacaga aatggttggc tctcttaatc atctgatgcc tttaggattg
    34321 gctgctcttg ttgcttatat cgtcaatgac cttctcggcg gtaatccaat ttacgaatca
    34381 ttattggaac gtctacttgg aaatactcag accgcctcac taaaaggtgc aaggacaact
    34441 tttgattttc cagtaacggc agagagttct ttagacggat taatggtacg agacttcaat
    34501 tggcctaaaa atatgctgct aatctctata cgcagaggaa atcaggaaat cttgactcac
    34561 ggtgatacga tcatgaatgt aggagatatt ttggttatct tgactgatga agcaaacttg
    34621 ccaagaatca agcaggaaat cgaggctaaa tcttctatca caaatttgaa gcaatcattt
    34681 taacgaacat ggaaaaacta ttttacaaca actattcatc tacactactt gattctatat
    34741 gattctataa aaaaagaaga ggctgggaaa a
//
LOCUS       DADVXU010000015        33967 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.016, whole genome shotgun sequence.
ACCESSION   DADVXU010000015 DADVXU010000000
VERSION     DADVXU010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 33967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.016"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(28..1260)
                     /locus_tag="KB953_000760"
     CDS             complement(28..1260)
                     /locus_tag="KB953_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBB6865756.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1515..2084
                     /locus_tag="KB953_000761"
     CDS             1515..2084
                     /locus_tag="KB953_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBB6865757.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2262..2702)
                     /locus_tag="KB953_000762"
     CDS             complement(2262..2702)
                     /locus_tag="KB953_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBB6865758.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2860..3624
                     /gene="map"
                     /locus_tag="KB953_000763"
     CDS             2860..3624
                     /gene="map"
                     /locus_tag="KB953_000763"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBB6865759.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3656..4579
                     /locus_tag="KB953_000764"
     CDS             3656..4579
                     /locus_tag="KB953_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HBB6865760.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(4804..5043)
                     /locus_tag="KB953_000765"
     CDS             complement(4804..5043)
                     /locus_tag="KB953_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6865761.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(5048..5467)
                     /locus_tag="KB953_000766"
     CDS             complement(5048..5467)
                     /locus_tag="KB953_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBB6865762.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            complement(5694..6932)
                     /locus_tag="KB953_000767"
     CDS             complement(5694..6932)
                     /locus_tag="KB953_000767"
                     /inference="COORDINATES: protein motif:HMM:NF017760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB6865763.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            complement(7039..7674)
                     /locus_tag="KB953_000768"
     CDS             complement(7039..7674)
                     /locus_tag="KB953_000768"
                     /inference="COORDINATES: protein motif:HMM:NF012655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBB6865764.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            complement(8016..8651)
                     /locus_tag="KB953_000769"
     CDS             complement(8016..8651)
                     /locus_tag="KB953_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6865765.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            9163..10305
                     /locus_tag="KB953_000770"
     CDS             9163..10305
                     /locus_tag="KB953_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBB6865766.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            10298..10504
                     /locus_tag="KB953_000771"
     CDS             10298..10504
                     /locus_tag="KB953_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBB6865767.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            10583..11776
                     /locus_tag="KB953_000772"
     CDS             10583..11776
                     /locus_tag="KB953_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBB6865768.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            11981..13120
                     /locus_tag="KB953_000773"
     CDS             11981..13120
                     /locus_tag="KB953_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HBB6865769.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            13113..13913
                     /locus_tag="KB953_000774"
     CDS             13113..13913
                     /locus_tag="KB953_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBB6865770.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            13913..14740
                     /locus_tag="KB953_000775"
     CDS             13913..14740
                     /locus_tag="KB953_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBB6865771.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            14718..15452
                     /locus_tag="KB953_000776"
     CDS             14718..15452
                     /locus_tag="KB953_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBB6865772.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            15552..16418
                     /gene="rfbA"
                     /locus_tag="KB953_000777"
     CDS             15552..16418
                     /gene="rfbA"
                     /locus_tag="KB953_000777"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBB6865773.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            16432..17004
                     /gene="rfbC"
                     /locus_tag="KB953_000778"
     CDS             16432..17004
                     /gene="rfbC"
                     /locus_tag="KB953_000778"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HBB6865774.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            17026..18054
                     /gene="rfbB"
                     /locus_tag="KB953_000779"
     CDS             17026..18054
                     /gene="rfbB"
                     /locus_tag="KB953_000779"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBB6865775.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            18152..19003
                     /gene="rfbD"
                     /locus_tag="KB953_000780"
     CDS             18152..19003
                     /gene="rfbD"
                     /locus_tag="KB953_000780"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HBB6865776.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            19037..21070
                     /locus_tag="KB953_000781"
     CDS             19037..21070
                     /locus_tag="KB953_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865777.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            21114..22394
                     /locus_tag="KB953_000782"
     CDS             21114..22394
                     /locus_tag="KB953_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HBB6865778.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            22604..23410
                     /locus_tag="KB953_000783"
     CDS             22604..23410
                     /locus_tag="KB953_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6865779.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            23422..24642
                     /locus_tag="KB953_000784"
     CDS             23422..24642
                     /locus_tag="KB953_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6865780.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGVNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            24632..26218
                     /locus_tag="KB953_000785"
     CDS             24632..26218
                     /locus_tag="KB953_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBB6865781.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            26257..28395
                     /locus_tag="KB953_000786"
     CDS             26257..28395
                     /locus_tag="KB953_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBB6865782.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(28763..29746)
                     /locus_tag="KB953_000787"
     CDS             complement(28763..29746)
                     /locus_tag="KB953_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HBB6865783.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            30124..31515
                     /locus_tag="KB953_000788"
     CDS             30124..31515
                     /locus_tag="KB953_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBB6865784.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            31531..32571
                     /locus_tag="KB953_000789"
     CDS             31531..32571
                     /locus_tag="KB953_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBB6865785.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            32590..33675
                     /locus_tag="KB953_000790"
     CDS             32590..33675
                     /locus_tag="KB953_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HBB6865786.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            33708..>33967
                     /locus_tag="KB953_000791"
     CDS             33708..>33967
                     /locus_tag="KB953_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBB6865787.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEK"
BASE COUNT        11009 a         5823 c         6698 g        10437 t
ORIGIN      
        1 attttttgca tgtgtgcgaa agtatactca agcccaatcg ccatttcgga aaatcggtac
       61 tgtagagcca tcttcacgga ttccgtcgat atccatcttg tcagatccta ccatgaaatc
      121 tacgtgggtt tgacttctat tcaatccagc ttctgctaat tcttcatcgg acatttctgt
      181 accgcctttc acactgaagg cataagcaga acctagtgct aagtgattcg atgcgttttc
      241 atcaaataat gtattgaaaa agatgatgcc agattgagaa ataggtgaag ggtcaggtac
      301 aagtgcgact tctcctaaac gacgcgcacc ttcatctgta tcaagcagtt ttgctaagac
      361 atcttctcct tgttcggcag aaaaatcaac aacttttcca tctttaaaag taaatttcat
      421 acccgagatg atggtgccag cataactcag tggctttgta cttgaaatgt aaccatctac
      481 tcgatgactg tcaggtgctg tgaacacttc ttccgtcggc atatttgcca tgaatttttc
      541 tccacgagca ttatagcttc cagcgccttc ccaaagatgg tttttaggca atccgataat
      601 gatatctgta ccgggagcag tatagtgaag ggcagaaaat tgttctcgat tcaattcttc
      661 tgcttttttt gctaattttt catcatgctt cttccatgct aagacagggt cttcttcata
      721 gacacgagtc gttttgaaaa tctgatccca taatgcctca acttgttttt cttctggcag
      781 ttctgggaag acttttgctg cccattgttt accagccgca gccacgacag tccaactgac
      841 tttatttgct tgagttgcct tgcgtaaatt cattagtgct ttgccattcg ctgattgata
      901 agacgctact cgatggctat ctacaccagc aaatgcatcg ggatcggcag aaacaacgct
      961 gatcctgctt gctccttttt caagccattc atccgcttga tcgattttag attgtggtac
     1021 attctcaata cgatccgttg cagcatgtaa aagaaattct ctttggatct ggtcgtcagt
     1081 ccattgaacg ataacctcag cggctcctag tttataagct tcttgtgtga tcaatcgagc
     1141 aaggggtgcc tgttccacgc tgatctgtaa aacaactgtg tgtccttttt ctgtggctac
     1201 gccagtttct gcaattaagc gtgcatattt ttttagaagt tcattaaaat cagataacat
     1261 aataaaaatt cctcctcacc attttttctg gtaactatta gcatgatacc atttaattat
     1321 gattcaaaca atgaggcttt aaataaaaaa aggaggttat ttttttaagg ttaagtttca
     1381 tgatatatgt tactacttgc tatttcagct tgtgaagaaa atcaaaaact aattttgcat
     1441 atcaaaaaaa acatgttaca atgaaattaa tcaatggtgg aagcgttata aaaaaattaa
     1501 ggagattaaa aaatatggca agaaaaaaga cgattactag agaacagatt ttggcagctg
     1561 cttatgaagt agctgccacc gatggattta cacgatttac tgcgagaaac atcgcgaaca
     1621 aaatgaattg ttccacgcaa ccgatttatc ttgagtttaa aaatatggat gacttgagac
     1681 aagctctttt tgagcagatc tatgatcatt tacaatatga agtgttcact gttgagcata
     1741 cagggaatac gattatcgat cttgctttta attatatcca ttttgcaaga agagagaaga
     1801 agttgtatcg ttcactgtac ttagaagaat atggtggagg aaaagagatg cagaatttct
     1861 cttatactta ctttgtgaat acagtgaaac gcgatccagc ttatgtgaat ctttcagaag
     1921 agcagatcga atctttgtat aatgggacat ggatcatagc tactgggatc gcagctttga
     1981 tgtcttcaag catcatccat ccaactgatg aacagattga aaagatgatc caagacagta
     2041 tagatgctat cttgaaattc aaagaaccaa tcgaaatcga ttgaatatac tcaaaagttc
     2101 cacaaatcag taggtaacaa gagatgtgtc aaaataagga aaagttcgtt tttcttacag
     2161 cgtatgcatt tttaactccc gaagaactct tccatgaatg caatgaataa aagagccata
     2221 cagattgaat aaatctggat ggctctttta ttgcaagtat attaaatttt tgcgactagt
     2281 tctttagcga aagcttctaa attttcgata tcttcttctt cagcattcag atccacttta
     2341 acattttcgg ctccttttga tgcgcctgtt tttgcaaaag cagcatcgaa atcatccacc
     2401 gatttgcaga attcatcgta aaaagtatca ccagatccgc agacaccgta aatttttccc
     2461 aatagatcca attcttgcaa gtcttcataa aagtctacga tctcatcagg cagatcgcca
     2521 tcatcgtatg tataagtagc tactacgcaa atatctgcct cctcgaattc atctgcatct
     2581 acttgggtac attcgttgat ttcgacttcg atatccagat tttcaaatgc ttctgcaaca
     2641 atatcggcga tttcttcagt gtttcctgtc atgctagcat atacgatttt tgctaaagcc
     2701 atgatgtgcc tcctcgttta taattattta acaattatga attataccaa aacatcattg
     2761 aaaagaaaat aaaacctaga gacacctggg gaatgtcatt tggctaaatg tatgccgcta
     2821 tgttaaaatg agagggattt gtaaaaagga gacgaaaata tgattacttt aaaatcagca
     2881 cgtgaaattg aaatgatggc agaatctgga gctttattag cagatgtaca taaaaatctg
     2941 cgtgatttta taaaaccagg aatcacaagt tgggatattg aggtatttgt cagaaactat
     3001 atcgagagtc atggcggaat tgctgcgcaa attggctttg aagggtatga atatgcgact
     3061 tgtatcagta taaatgatga aatttgtcat ggattccctc gtaaaaagcc tttgaaaaac
     3121 ggtgatttgg taaaagtcga tatgtgtatc gatttgaaag gcggcatttc tgattcttgt
     3181 tgggcctatg tagttggaga atctacacct gagatcgacc gattgatgga agtaacgaag
     3241 aaagctttgt acatcggtat tgagcaagca caagtaggga atcggattgg tgacatcggt
     3301 catgctatcc aaacttatgt agaaggcgaa aatctagcga tcgttcgtga ttttattggt
     3361 catggcgttg gtcctacgat tcatgaagaa ccagcagttc ctcattacgg agaagctgga
     3421 aaagggttgc ggttgaaaga aggcatggtt atcacgatcg aacctatggt taataccgga
     3481 acttggaaaa tgaaaatgga tcctaacggc tggactgcgt atacaagaga cggcggactt
     3541 tcttgccaat atgaacatac attggctatt actaaagatg gtccaagaat tttgacatca
     3601 caaggagaag aaggcactta ttgatctatc cagtcaatag gtacgggaga ggtacatgaa
     3661 agtttggaac aaaataaaac aaaatgaaaa tctgatgcgc tttattgaga cgactcaaaa
     3721 gcgcatcact gattcagaaa tgagtacgac ctctgtcgtc gttgcttatt atttactctt
     3781 gtctttattt ccattgatta ttgcttttgg taatatttta ccgtttctcc acatcgatca
     3841 agagactgta ctgacctatt tacgagaagt gattcctgag accgtttacc agtttatcgg
     3901 tcctgcaatc aaagaccttt tgacacaaag ttcaggaggc ttgttatccg tttctgcatt
     3961 agcggcttta tggtctgcaa gtcaaagtat caatgctttg caaattgcga tgaataaagc
     4021 gtatggcgta gaaaatagaa aaaatttttt gatcgtccgt ttcttctcac taattgtcat
     4081 tgtcttgttc atgattgcta ttagtggggt aactatcgtt cttgggttgg gacagctgat
     4141 tttggatgct attcagccaa tcgtacaaat acccgtgaac tttatcgatc agttccaaac
     4201 tttgaagtgg ccaatcacat tggtagcttt atttgtcatc atgttcttga tttatttaat
     4261 tgtccctaat gctcagttga agttgaagac gatcattccg ggcacgatct tcgctactgt
     4321 cggctggatg ctgttatcac aagtgtttag tatctatgct aggtattttg caacgaggat
     4381 cagcggttat cagatcatag gaagttttat tgtactcatg ctttggctta attttgctgc
     4441 tacgatcatc attttgggcg gaatcatcaa tgcagttgtt caagaatacg tgacgggtca
     4501 tcaaatcaaa aaacgtcgag atccaagccg aaaatggttc aataaattga aaactaaatt
     4561 ttctcataaa aaaaagtaga aacaagagtt cttattgtaa atttatatca gtaatttatt
     4621 tgaaatttta tatctaattt tcaattatcc gcataaacac tatacattca agcattttga
     4681 agtatagaaa aacactcatc ttaccacgaa tttaccacga ttgaatgagc cttgatatga
     4741 ccgtaaaact actaagggag atagcacgaa actgtgtatc tcccgatttt tttatacagt
     4801 tgattacttc actccgattt caaatttcaa tattgctttc atcatgtctg tatgaatttc
     4861 tccttttagt tcttcatcaa cctgcataat gattttacca ctttcagagt ggtaaaaagg
     4921 acgcaagcat aatttagaaa tataaccgtc ataaaacttc aaaatctctt taactgccat
     4981 ttcatctccg cttgacgctt tgcaaatcaa ttcaaaagag ggatagccat aatctttact
     5041 catttcttta cttcctttcg ctcaaaagca tttttaactt ccttagtcct ctagttcttc
     5101 tactgcaaat agcagaacgt gatacatggt acagttctga aatttctctg tcgttcattc
     5161 cttgaaaata acgtaataaa atcgcactac gctgtttttc tgacagtcta tgtaaaactg
     5221 taccaagtgt ttcatcagat acataaattg taaagtgcaa gactttaaat gatacacaat
     5281 cataaaaaca ggtatcaatc gttccacgtt caacttgttt cgtttcggct atttcacaaa
     5341 acaatacttc acgtttagaa tgtcttgcaa gctgtctgtt tcgacttttc actttgttct
     5401 taatgacaat cagcattaaa gaattgaact gtaatctgac gatatgctcg aaagaagatg
     5461 tcctcataat ctcaccccct ttctgctaat gctgaaatat ataaacggaa taccgtttat
     5521 accctttaga aatacagaaa aatggtaaga aaaaagccca cacaaaacat atatttgtgc
     5581 gagccttaaa tactgcaata ttactctttc ttttataaca ctatcctttt agcagatttc
     5641 aaattttctt ttatatattg tttttagcct aacatactat ttaacccctt gtattatatc
     5701 tctaactttt gaaaaattct ttttgaatat attgaaatca tgattgaagc aatagaagct
     5761 acaaacataa caacccatgt ttcttgtata aatacatcga ataaaatacc gtaaaccatt
     5821 tgtccaatag gttgaccgca catagcaact gctaccagcg tagccattat ctttcctact
     5881 aattgcggtg gtgtttgtgt ctgaacaaga gtgtaaattt gaacaacaaa catagtagat
     5941 acacccatag ccacaaagca catcactgta actacccagt aggaaactat tgcaggcaat
     6001 tctaaaaata gcgaaattcc cataagtaaa acagaaacag aacaaataag taacaaggta
     6061 taaaggtttt ttagttttaa ctttgaagca acaagagctg ttaaaatacc accacataaa
     6121 ccacctaatg ataatgctga ttgtgaaaaa ccatatagtg tatctggcat ttttaaaact
     6181 tcagaaatta agataggcag accaacaatt aaaacagcac ttaaaaccaa attgaaaatg
     6241 caaaccaaaa agattacgga tataaaaatg ggtttctcct ttttcaaaaa ggtataacta
     6301 tcttttaaat cgtttttagc aactgcaatt acacctactt cacgaggtct tttttggtgt
     6361 ggaatatgga taaaaatttc cataatggct gaaaaagtaa aacatataat actaacaatt
     6421 aaaatgggat tgataccata aaattgaaaa actactccgc ttatagctgg tccgataaag
     6481 tttgctaaag tatttacttg atttattata gcattaccga cggtaagttt atctgatgat
     6541 accaatagag gaatacttgc ttgtaccgct ggttgatatg caccagaaat accataaagt
     6601 aacatcaaag caacaataaa caaaggtatg ataggaattt ttcccaatga caaataaaat
     6661 actaaaatta tgattgctgt tacaaaatct agtatgacca taatgtttct tttatttgtc
     6721 ctgtcagcaa taataccacc taccagcgac aaaataacca taggtaaaaa agagcaggct
     6781 gtagcaatac caaacaaagc tgatgaacca gtttctttta acaaatagta cggtaatgca
     6841 aatcgtagga tagcgtttcc aaataaagaa attatctgtc ctataacaac aagtataaaa
     6901 tcacgattaa aaagtttttt ttctgcttgc ataaaatctc tcctttttat ttaatcaaac
     6961 agaccgtcgg tttatataat ggtttaaata aactgctctt ttatagagca gtctatcgta
     7021 tagggataac aaatacagtt atttacaaaa ctgtataaat gcagaaattg tttcttcgtc
     7081 taataatgtg tctatgccta ttccatttaa aagggcattg aatgtattgc aacgtttttc
     7141 cataccctct ttatccgtca tttcaactaa tgggttgagc catacattac ttaataccat
     7201 gataacttct gcaatttcat gtgaattttc aatctccaaa gacttatcct tgataccttg
     7261 ttctaaaacg ggttcaacaa attcaggtac tacaatttca tacatttgtt ctatttgcat
     7321 agcaagaaaa cgaggattgt ttaatagttg aggagaaact gtcaataaaa cgctttggtt
     7381 agaattgaat accgctgtct taaatatttc tcttaacttt tctaaaccgt ttaaactatc
     7441 gttatctcta attttagcaa atattactgc attttcattt ccaattcttt gaaaaattgc
     7501 ttttaaaata tcttcctttg aatcgaaatg atgataaata gcacctttag aaagttttgt
     7561 ttcattgata atatcttgca atgatgtgtt atcatatcct ttttcacaaa acatttttgt
     7621 agaaacatct aaaatgagtt ttactgtttc ttctggatat ttatttcgtg ccataactta
     7681 cctccaatac aaaccgttgg tctgattaaa gtatatctta gtattttatg cttgtcaata
     7741 taaagacttg caattttata aaaatgcaga atgcaagcct ttttcattta ttctacaatc
     7801 ttccagttgc tttccttatg cggtgtcaat tcatactgtg atacctgcgt tgcctttgcc
     7861 tgatttacca caaatcagat tttatccctt tcttcctctt gattgcccac aacagaaaag
     7921 aaaaagccct gtcaagagct ttgccaccat tggtggtgct ttgcactctt gatcgggctt
     7981 tttgtttgct gtatcttctc gcaagaggaa aaaagttact cgtcctcatc atcaaaattg
     8041 ttattcctta atacttctcg taacagttcc atatcttctt ttgagttcgg gtttatcatt
     8101 ttcacgactt gataaggtgt cttatactta tgtccctcta tgctttcccc aaacatatcc
     8161 atactgtata aatcatcata gcggtctaat aatttttgaa acttcaaatg ctggataaat
     8221 tctttgattg ggtaaaggtc ggacgcttca atgcttttcc cgttgatata gtctgtaata
     8281 tattcccgta acttttctaa ctcatattcc agccattctt cctcgctgtc aaagaagtta
     8341 tcatagtgtc catgtttcag ttctgcattt agacgttctt cggctcttaa aaactgacag
     8401 acttcctttt cagattgatt gacatacttc cattctcccg acaacaattc attgggcgta
     8461 acgtccagca cttccattat cttttccagc gtttcatagg caggatagtt caaccctctt
     8521 tcaatcttgg aaaggctctg catattgata ccgattttgt ccgcaagttc ctgttgtttc
     8581 attcccctaa attttcttat ggtctgtata ttatttccta aatggcttat tttcattgca
     8641 taaccctcca tatctcgtta tatcttcgct attataagtc tatcatgctt aatatgattt
     8701 gtaaaggcaa aaaaagatat tgacaaataa gcatataaga attactatgt tcttgtaatc
     8761 gaataagcac aatagaatga aatataaaaa cattcaaaaa gaaataccta tctgattaaa
     8821 tccgtagata tgaatatcgg ttgaaatgga acttgttaga aaaggcaaaa cagaacttgc
     8881 tgacaaagta tttctgacac ggtggcggaa gcctctcagg cttaccgcca tgacaccttg
     8941 tgtcaagggg aagaaatgct ttgtcggctg gcgagcctgc gagccttgtc tgtgcccccg
     9001 ttatctaaca ggggggcact atttcacaaa agacaagtca aaaaccatac acccccaaac
     9061 ctgtgagtag taatgagagg ttttaagaag atagtacata tcacactttt tgaaaggtgg
     9121 tttacaggta tttcaaggct attgtgggat tggaggaata caatgaacaa tttagaattt
     9181 gcacttgaaa tgaagaacaa acgtcttgcc agcggtcttt cacaaggaga acttgcaagt
     9241 cttacccatg tatcaagata tagtatcaac cgctttgaga acggaaaagc taacgccagc
     9301 aaggaaacac agaacattat cctgcgttgt ctgaattact atatctgcga taagcctttt
     9361 tatctgctcg ttgattatct gtctgtccgt tttccaacga ctgacgcatt ggaagttatc
     9421 cgaaaggtgc ttggcatgaa agcagattat tttatccatt atgactatgg gtattatggc
     9481 tataaggagc attatgctta cggggaaatc aaggtcatgg cttctgatga tgaacacatg
     9541 ggcgtatttt tagaattaaa aggggcaggc tcacgcaaca tggaatatgt cttacaggca
     9601 cagaacaggg actggtattc attcttaaac cgctgtcttg actgtggcgg tgttatccga
     9661 cgttttgact tggcaatcaa tgatatgtgc ggtctgctgg atattcctgt gctatctgaa
     9721 aaatacagaa atggtggtgc ggaatgtcgc tgtaaaaact atgaaaatgt acaaggtgga
     9781 aaattaagcg gaaaaaaccg taatttagca agtacccttt atatcggctc aaaggcaagc
     9841 acaaaatatt tctgcctgta tgaaaaacaa aaggaacagg caacgaaaaa gaaacatacc
     9901 gatattatca accgttttga aattcgtctg cgtgacaaaa aggcggtaca ggcagttgaa
     9961 gaactgttat tgacgtataa ccctcatggg ctggtgtttt atctcattac cgattttgtg
    10021 gaatttcctg actatccatt gtgggagata ttcatttccc atgacagttt accttttgaa
    10081 atgaaccctg tacctgtcaa tatggaacgc actctgcaat ggctggaaag acaggtcatg
    10141 ccgtcaatcg tgatgataga ggaaatcgac aggctgacag gttcaaacta catgaaaatg
    10201 attgatgaat gtacccacct ttctgaaaaa caggaaatgc ttgtggaaca gatgtgtacg
    10261 gacatatcag acgtaatcga aagtgagggg gtgttttatg agtaatacac aggatattcc
    10321 cgtatgggaa aaatacaccc ttaccattga agaagcgtca aagtatttcc gcattggaga
    10381 aaacaagtta agacgattgg cagaggaaaa caaggacgct ggctggctca ttatgaatgg
    10441 caaccgcata cagattaaac gccgacagtt tgaaaaggtt attgataaat tggacgcaat
    10501 ctaatgcaaa tgagccttgt atgtgttatg atgaacacaa gtcatatcaa ggctctttcc
    10561 aacaaggaaa ggagcagaca ccatgaaaga aaaaagacgg gataacaaag gtcgtatcct
    10621 gcatactgga gagagccaac gaacagacgg aaaatactta tataaatatg tagacgcatt
    10681 gggaaacaca aaatatgtgt atgcttggag attgacaccc acagacccga caccaaaggg
    10741 aaaacgggaa aaaccctcac ttcgagaact ggaacagcag ataagacggg atattgagga
    10801 cggtatcgac agcacaggca agaaaatgac gctttgccaa ctctatgcca aacagaacgc
    10861 acagaggaca aatgtgaaga aaagcacaca gaaacaacgg gaacaactca tgcggttatt
    10921 aaaagaggac aagttaggtg ctaggagcat tgatacgata aaaccctctg acgctaaaga
    10981 atgggcgtta cgcatgaaag acaaaggctt ttcctacaat accattaaca accataaacg
    11041 ctcgttaaaa gcgtcattct acatagccat acaagatgat tatgtgagga aaaacccttt
    11101 tgatttcaag ctaagtgaag tcatagaaaa tgataccaaa gagaaagtcg cattgacaga
    11161 ggaacaggag caagccttat tgtcatttat taagacggac aacgtgtatc acaagcatta
    11221 tgatgatgtg ctgatactgt taaagacagg acttcgtatc tcggaactgt gcggactgac
    11281 agtagccgac attgatttca agaatgaagt tgtgattatc gaccaccagt tactaaagag
    11341 taaggaacag ggctattata ttgaaacgcc taagacgaaa agcgggataa ggcaagtgcc
    11401 attaagcaaa gaaacgatac aggcatttca acgggttatc aacagccgac caaagataaa
    11461 gtcgatagag gtagacggac acagcggttt tgtgtttatc aatccgaaag gtaatccaaa
    11521 agttgccatt gattataacg ccttatttat ccgtatggta aagaaataca acaagcacca
    11581 caaggacaat cccttgccac atattacacc gcatacgcta cgccatacat tctgcacaag
    11641 gctggcaagc aagaacatga acccgaaaga tttacagtat atcatggggc attcaaacat
    11701 cagtatcaca atgaactggt acgctcatgc gtccatagat accgcaaaat cagaggttca
    11761 gcgtctaatc gcatagaagt atttaccacg attttaacca cgattgacag ggaaaatata
    11821 agaagataga cctagatatg cgaggtttac cacaaaagca aaatgcccgt agagccgata
    11881 aaataaggct ttgcggacat ttgagaagat ataaaaagat agtcaaaaag acatatataa
    11941 tttataataa ttatgactct taattaaagt aggtgcccat atgcttgttt cttttagtat
    12001 ggttataaga ttttttctga caattttatt atcactgata ttaacacctg tattcaaaat
    12061 catttcagta caaacaggta tggtcgacaa accaaatgaa agaagaataa ataaagttcc
    12121 aatgccttca gcgggcggac tgcctatctt tatttcattt gttatatcga ctttattctt
    12181 attccgtaaa attatccctc aatcgtatat tgtaccgatc ttgattgctt ctttagtaat
    12241 catagtcaca ggagtgctcg atgataaata tgagctgaca cctaaacaaa aaagtatagg
    12301 gatcttgatt gcttctctta ttatttattt tgtagcagat atacgtattg attccttcac
    12361 gcttcctttt attggatata tccaactagg ctggcttagt tttcccgtaa cgattttttg
    12421 gatcttcggt atcacgaatg cggtcaatct gattgacggt ttggatgggc tggcggcagg
    12481 gatttcattg atcggtttaa ttacgatagg aatcattgga tattttttcc tccatgcttc
    12541 tacagtgtat attccagttg ttattttctg tcttgtagca agtattatcg gtttttttcc
    12601 ttacaatttt tatccggcaa agatttattt aggcgataca ggagctctat tcctagggtt
    12661 tatgatggcc gtcctttcac tacaagggct gaagaacgtg acttttgttt catcgatttc
    12721 tttgctgatc gtcatgggag tgccggtaac agacacgttt tttgctatca ttaggagaaa
    12781 agcaaaccgt gtttcttttt ctacagcaga taaaaaacat cttcatcacc gactaatcag
    12841 tttaggcttt actcataaag gtgctgtcct gacgatttat gcgatggctt tgatgttttc
    12901 tttcacagct atggtcatga attatactgg aagaatcgga acgatcatat tgattattgc
    12961 catgttgttt gctgcaattt tattatttga attgattggt ctgatcaatg agaaacatca
    13021 gtttatttta aaaacattgc gatttttagg gaataaagag taccgtacgc aagtaaaaca
    13081 gaaatatttg aagaaatgga accgtcatat agatgaataa caaattaaac cagccgatcc
    13141 ttattagtat tgtgacggca aacagtgata aaatttttcg gacactagat aattttattt
    13201 caactgtaaa agatgaaaca gcagtaaaaa tccgaatttt tgacaacaat tcacttcccg
    13261 agtacaaaaa aagactaaca gagtatagga aatatcccta catcgatatc tattttcatg
    13321 atgaaaattc cggatttggt tatggacata atcacaatct gatagctagc gattctcgct
    13381 atggcattat ttgtaaccca gatattttag tggatgaaga gacagttaca gcattggtct
    13441 tgttattgaa agaacatccg gaatgtgcga tgttagcacc taagattctt aatgaagatg
    13501 gaacaactca gcacctcatc cgtgagcagc tggctgtttt tgattacgtt ttgcgctttg
    13561 tgcctttcaa atgggtcaaa aaaaagtttg acaaacgact ggctgtgttt gaatgccgag
    13621 gattgcctac tgatgctaat agttatgtac ggatgatctc aggtagtttt atggtagctg
    13681 atattgataa atttaaagag ataaaggggt ttgatgagcg tttcttcatg tactttgaag
    13741 acaacgactt gtgtttgagt tttgaaaaag caggttacaa gttattgtac acaccctttt
    13801 atgcagttat ccatttttac gggaagggtg cgcatcgaaa tgcaaaactg tttcgcgtgt
    13861 ttttgacttc tatgatgaaa tttttcaata agtgggggtg gcgttttttc tgatgaaacc
    13921 agaaatatta gtagtcgttg tgctttatca gcaactattc agtcaatctc cagcttatgg
    13981 accattaagc aaagcactta gcgaaaagca aattcagctt attatttacg ataatagtcc
    14041 tcaaaggcag caaaatgagc tgtttgaaaa agaaaatatc gtttatcatc atgatccgca
    14101 aaatcctggt ttagcggtgg cttataattt tgcgttgtct caagcagatg aaggaacacg
    14161 ttgtctcgtc actctggatc aggatacgag gctagtcgac gattattttg aactattgcg
    14221 taaagtaacc tttacagatg aatgtgttgc tgcagttccg atgatttttt ctgggagccg
    14281 acaagtttct cccctatatt cagatcgtta tatcaatcgt acagctcaag cagtagaagt
    14341 tgaaggccca acagctgaac gaatcatggc aatcaattca ggtgttgcct ggtctgtcaa
    14401 atttctaaaa aagattggcg ggtttaatcc tgctttttct ttagattttt tggatcattg
    14461 gctgttttgg aaagtggatc agttggaaaa aactgtggag atccttcctg tacgaatgga
    14521 acatgatctt tcggttttag actatgaaaa agtttcaagt aatcgatacc gttctatttt
    14581 acaagcagaa aatcgatttt atcatgaata tgatcaaagg catttatcca gtcaccgacg
    14641 acaattattg ttaagaacag ctaaacagtt cttgaccgtc aaaaaccggc atatctggcg
    14701 gatgactttg cgctcatatg ttaagaattg gaaggtgtga attcgtgcgt tcggtttgta
    14761 tagctacctt taatggaggc agatatttaa gagagcagtt agatagtatt ttatctcagc
    14821 tttcagcaga agatgaagta gtcatttctg atgatgggtc agtagatgat acatgggata
    14881 tattaactga atacgcagaa aaagataagc gaatccagct tttttcgggg cctcgtcaag
    14941 gattgatcgc taattttggt tatgcgatcc agcagacgaa aggagatcta atctttttgg
    15001 cagaccaaga tgatgtttgg ttggagacaa aagtgaaaaa gatcactgac tattttgagc
    15061 agaatccgga tcagctagta gtagtttctg atttaatcat tgtggacagc gagctgcaag
    15121 agatccatga ttcgtatttt tcgtttcgaa attccaaaga aggatggctt aataatgtga
    15181 taagaagtca ctatattggt gctggtatgg ctttccggtc ttctttaaaa cccgatattt
    15241 tgccgatccc gtcagaagtg ccgatgcatg atatgtggat tggtttgctg ggaggaaaga
    15301 agactgggtt cctaagagaa ccgttaactc tgtaccgaag acatgattta aacgcgagtg
    15361 agattgaaac acattctaaa ttacggcaaa aaatttcttg gcgtgttcat ctgattacgg
    15421 cgttgatcaa acgaaaatgg tttaaacgtt gataaaagat gtttaaatcc ttaatatttc
    15481 catgacaaat ataacaattg atgagataat aacaaagtga aatttgaaat caaaaaggag
    15541 agcagatatc catgaaggga atcattcttg ctgggggcag cggaacacgt ctgtaccctt
    15601 taacaaaggc aacatcgaaa caattgatgc cgatttatga caaaccaatg atttattatc
    15661 caatgtctac tctgatgttg gcgggaatca aagaaattct gattatttca acgccacaag
    15721 atacaccacg tttcaaggaa ttatttggaa acggaaatga tttaggactg cagatcgaat
    15781 atgcagtaca agaaagtcca gatggattgg cacaagcgtt tatcattgga gaagaattta
    15841 ttggagatga tagtgtctgc ttggtattag gggacaatat ttactatggt ggcggtctgt
    15901 ctaaaatgtt gcaacgagca gcttcaaaag atacaggcgc gacagtattt ggctatcatg
    15961 tcaatgatcc agaacgcttt ggtgtagtag aatttgatga gaatatgcaa gctttgtcga
    16021 ttgaagaaaa gcctgctcag ccaaaatcaa attacgctgt aactggcctt tatttttatg
    16081 acaatgaggt aatttctatc gctaaaggta tcaaaccttc agaacgtgga gaactagaga
    16141 ttacggatgt caataaagca tatttggata aaggaaaact atccgtagaa cttatgggac
    16201 gcggatttgc ttggcttgat acaggaacac atgaatcatt gcttgaagca tctaccttta
    16261 tcgaaacaat tgaaaaacgt cagaacttaa aagtggcttg tttggaagaa attgcttacc
    16321 gtatgggcta catcgataag gaacaattgc tggcccttgc acagccattg aaaaaaaatc
    16381 aatacggtca atatttattg aatttggccg ctgaataagg agtccttaca gatgaaagta
    16441 acagatacaa aattaacaga tgtcaaaatc gtagaaatgg acgtctttgg cgatcatcgc
    16501 ggtttcttca cagaaagtta ttcaaaagca aaatttgcag aacatggatt gaaatatgac
    16561 tttgttcaag acaaccattc actatcagct gaagctggtg tccttcgggg gcttcatttc
    16621 caaaaaggag aagctgcaca gacaaaattg attcgggtag tcactggagc agttttagat
    16681 gtagtagtgg atatccgcaa aggtagcccg acttatggtc aatgggacgg atttatctta
    16741 tctgaacata atcatcgaca gttgcttgta cctaaaggat atgcccatgg attcgttact
    16801 ttgacaccaa atgtgaactt cctttataag tgtgataatt actataatgc agaagcagat
    16861 gggggcatcg catttgatga tccagattta gcgattgatt ggccaattga tccatcaaga
    16921 gcgattatgt cggaaaaaga tcaccgtcat ccgacattaa aagaatttga agcagaaaat
    16981 ccatttgttt acggagaaat ctaagaatta aggagaaatt ttcttatgaa aaacatcatt
    17041 gttaccggag gtgccggatt catcggctct aatttcgttc attacgtggt aaacaatcat
    17101 ccagaagtgc atgtcactgt tttggataaa ctgacttatg caggaaacaa agagaacttg
    17161 gctggacttc ctagcgatcg cgtagaattg gtcgtaggag atgttgcaga tgcagaatta
    17221 gtggaccgtc tagtaaaaga agcggacgca gttgttcatt acgcggcaga atcccataac
    17281 gataattcat taaaagatcc tttcccattt gtacaaacga atatcattgg tacgtataca
    17341 cttttagaag cttgccgcaa gtatgatgta cgctatcatc acgtatctac tgacgaggtt
    17401 tacggcgatc ttccattacg tgaagacctg ccaggacatg gggaaggcga aggagaaaaa
    17461 ttcactgcgg aaacaccata taatccttca agtccatatt cttctacaaa agctggttca
    17521 gatctgttag taaaagcatg ggtgcgttct tttggcttac gtgcaaccat ttcaaactgt
    17581 tcaaacaact atggtcctta tcagcatatt gaaaaattta ttcctcgcca agtaacgaat
    17641 atcttaagcg gtattcgtcc aaaattatat ggcgaaggaa agaatgttcg tgactggatc
    17701 catacgaatg atcattcttc agcagtgtgg ctgattttga cgaaaggcag aattggcgaa
    17761 acctatctga tcggtgcaga cggtgaagaa gataataaga ccgtgatgga attgatctta
    17821 gaaatgatgg gtcaaccaaa agatgcgtac gaccatgtaa acgaccgtgc aggacacgac
    17881 ttgcgttatg caatcgattc gacaaaatta cgggaagagt taggatggaa gcctgagttt
    17941 acgaacttcc gtgatgggtt ggctgacacg atcaaatggt acgaagaaca tgaagactgg
    18001 tggaaaaaag aaaaagaggc cgttgaagca gcatatgcca aaaatggaca ataatacaga
    18061 gaaaatgtca ctgtattaat ggaaaaacac atgtgaggca ggcgctcgcc tgtctctttt
    18121 ttaaaataaa aatcaaggag cggataatca tatgttttta ttaactggag gaaatgggca
    18181 actaggtaca gaattgcgcc atttattaga tgaaaaaaat ctgcaatatg tttcaacaga
    18241 tgcaaaagac ttggatatca cagatgccga gaaaacaatg gcttacatca cagaattaaa
    18301 acctgatgtt attttccatt gtgcagctta cacagcagta gataaagcag aagaagaagc
    18361 aaaagaatta gatgagaaga tcaatgttga tggtacaaga aatgtagcac ttgcagcaaa
    18421 agctgtgaat gctcaattcg tctatatcag tacagactat gttttcgatg gaaaaaacaa
    18481 aaatgtagaa tacgaagtag acgaccaaac gaatccacta aatgaatatg gtcgtacgaa
    18541 attattaggc gaaaaagcag tagaagagat tttagaagac tattatatca tacgaacttc
    18601 ttgggttttc gggatctatg gacacaattt cgtcttcacg atgcaacgcc tagcggaaac
    18661 ccgcgacaaa ctgactgtag taaatgacca gtttgggcga ccaacttgga cacgtacttt
    18721 ggcagaattt atggtatatg tcatcgaaga aaaagcacca tttggtattt atcatttgtc
    18781 gaacgaaaat agctgttctt ggtatgaatt cgcaaaagaa atattgaaag atacagatgt
    18841 agaagttgca cctgttacgt cagaagaatt tccgcaaaaa gcaacacgtc cgcagtattc
    18901 agtcatgagc ctgaaaaaga cagaagagtt aggatttgtc ataccaactt ggcaagaagc
    18961 acttgcgcaa atgctggaag ctgccaaaaa aatgaataag taatttcctt ttatcaaata
    19021 gaatttggag catgatatgg aaaaacaaac aagaagtgca cgacgaaaag aaaagccaaa
    19081 agcgaaccag gcagattcga atttggtgct tgacctgttt ctaaaactcc gctatcttat
    19141 tggcgtttta gtcatcgtgg tcatcgttac ttttaattta aacggaagtt cacttggtgt
    19201 ttgggataaa tatgtgtcgc aaagagacga tgggaaaaaa actgatgtga tttttggaca
    19261 gaatcgggaa gtgcggtcag acgaatggct ggtgcagaca cctttttacc tctcacaagc
    19321 tgaagaggac tttccgttgg tgaacgagga ttattcatta aacggacaaa acatgatcat
    19381 tgcgtataat tctcctgtaa aggatattac ggttatcgga aaaccattca actggggatt
    19441 ctttttcctc ggaagagatc gaggactttc cttttattgg gcgatgaaat tagtcgtcat
    19501 ggtcttgctt ggattcgaag tactgatgat attaacgaag agaaaaaaag ctctatcgct
    19561 aatcggtgca tttgcgctga cattttctcc agcagtccag tggtggttca tgcaacatgt
    19621 gggcgatttg attttcttta ctttgggact gatgactgct ttttatcatt atttctacaa
    19681 tcatgataaa aaatggctac gaacgttgat gatgcttttg gtggttattt tcggtattgg
    19741 gtttatttta gtgatttacc cagcccatca agtgatgctg gcgtacttgc tgatttttta
    19801 ttttgttggc ctgcttattt actatagcaa gaaagtttca tgggatctgt tggatgtagt
    19861 gttgattgga ggggcagttt tatttattgg cggagtcatg gttcatttct ggctaacgtc
    19921 aaaagacgca ttgttagctt ctttgaatac cttatatcca ggaaaacggg taagtactgg
    19981 aggaaagtgg acgattggcg aatttttttc tttccttaca aattggaaaa ttccttttga
    20041 agatatcaac tattcgaata actctgaagt ggccctgttc tatcactttt ttccaactgt
    20101 attcctagca agtccatttg tgttatttgg caaaaaagat agcgaacaaa agttactggg
    20161 acgtattttg atgatttttt gtctgttttc tatcttttgg attaccgttg ggttgccaaa
    20221 atggctagca gaaatcactc tgctgtctta tgttcctcca ttaagagcca tccttacttt
    20281 tagttttgcg gcttgtttat tatcgatctg gtttatcgct tatatttgga gagaagatgt
    20341 tattcctatt tggtataaat tcattttgtt ggcagtaaat gcatggtctt atttctatgc
    20401 gattacgcat tcgaaaatgg ataattattt ttcggatttt gaaaccatcg cagttgctgt
    20461 gttgggaaca gtgattttag cttttgcatt atttggctgg agacgcttgt tttacctcgc
    20521 ttttgccgcc ttagttatcg tatctggctt tttcgtcaat ccagtcgtag aaggtacagg
    20581 agcgattttt gaaaaaacac tggcaaaaga aatccaaaaa atcgatcaac aagatcctgg
    20641 taaagtttgg ttgtctgaag atgaattata taacttcacc ccaacattgg gagtgaaatc
    20701 cttcaataca gtacgctttt atccagatat agctgcatgg aagaaaatcg atccgaaaga
    20761 agaatacgag aagtattata atcggtatgc tcatacaaga gcttttatcg ctaaggaaga
    20821 tacaaagttc attttagatc gtccagatat gttttcggtc acgttgaatt ttgaagacgc
    20881 agaaaaatta gggatcgact atgtgatcag taaacgacca ttaactgatt acaaccaatt
    20941 atatgaagcg cagttcacgc agctatatgg accggataaa gacggttata tgatttttaa
    21001 agtagaatat ccggattatc cagatgtacc acaaacacaa actcaggaaa atcagctagt
    21061 cgtacagtaa taggctagct gagaataatc ctgaaatgtt agtagaaagg agagtggaga
    21121 taattcaagc aatcgtacaa aaatttgtca acaaaatgag tgtcggtttc ttgcttttgt
    21181 tactgacgct agtctttttt gtttggacag cagggttagg ttttccagca actttgttta
    21241 tatataacaa tagttcatta cttatcgctt ttagtgtggc tattttgctc attttaaatg
    21301 tttataaagc aaaaggcgtc gattttcttt atctaggtgt tgcgcttgcc cttttcattt
    21361 tcttttctta ctttcgggaa ttaagaaatg agtctttatt tggagactta ctgtttcctt
    21421 tgattatttt attttgttta gtaattaaat ggatcgagtg gacaaaatgg gatcgcggta
    21481 tttatctgat tgtcttttcg gtcttcctcg gaatcacgtt atatcgtgta tttacagaga
    21541 tccaagtacc agaaggacaa agcatctggg ctccaggcaa ttcgctttct gatatttgga
    21601 tcaatacgaa tataatcgga agttctttga tgactcttgc tttattgatc agtggatttg
    21661 ccagttcttt tgaacgatgg tatgtacgaa tcatcggtgc agtagcagtt gttgcagctt
    21721 ttctcgggat ttgggtctgc caatctagag gtgcattagt cgcgttgatt gtttttgcga
    21781 tattggatct tttacctaaa tcgttgatgc gtgtcattcg tgcgccattt attgcttaca
    21841 cggttacgat tcttttagct ttgccgattt cttatctggc ggctgtctct gaaaaagtca
    21901 atttgtttac cggtcgagaa gatatttggc ataaattcta tcagacgcta ggagaaaaat
    21961 ctgaacaaat tttactagga atgaaaacat ttatcttcca acgaggaaac caatttttag
    22021 gaaaccacaa tagttataat tctattttga atatctatgg attgatagga tttgggattg
    22081 ctgctttgct gcttatcctt tttattggtc gcttaacgtt gaaagcagat ttatccaacg
    22141 ggcagatgac ttttatctgg gccttttttg cggtgatgat gcagtcattt atggaggata
    22201 cattgacttc atacccatgg gttccgatta tttatatcct gttggctatg gccgttcatc
    22261 gatatgatga accagaagtt gcggaggaat tctatgttat ggaagaggca tatgacgata
    22321 tgacttatga tgatacatct tatgaggaac aaccagatag cgctcattct tccaggatcc
    22381 aacgctatca ttgataggca caaaatacga gagaggctgt gacaaccgtc gcagcctttt
    22441 ttggtttatt gcaattgtct agcatcttct gtaaaataga aacgttagag aaaatcttgt
    22501 atcatagaaa attaagaaag aaataagaac gcagctcggt accaaaaata tggtaaaatg
    22561 gcacttgact gtaattcact gaaaatcgct tggaggaaca caaatgttta aagatttgta
    22621 tttatttatg aaggacattt atcagaacag aaaattattg ctacagtttt cgatcaatga
    22681 cttcaaatca agatatgcag gatcgttttt aggaatctta tgggcgttta tcaatccgct
    22741 atttactgta ttgatttatt ggctggtatt tggtgttgga ctgaaagcac aaatgatcga
    22801 tgcaaaatac ccatttatcg tatatttagt taccgggatg attccctggt tcttcttctc
    22861 agatgcattt gcatcaacta cggttgtttt tcgggaatat acttatttag taaagaaagt
    22921 cgtattcaat attcgtatct tgcccacgac gaaaatccta tccaatctgt acacccactt
    22981 attcttcatt ctgattggca tagctgtcgc gcttataaat ggtatatatc cttcagcaat
    23041 gtctttacag ctgatttatt atttattttg catgtgtgct ttcttgacag gattgacttg
    23101 gctgacggca tcgatccaac catttttacc tgatatcatg caattaatta cgatcgtgct
    23161 gcagttgatc atgtggacat taccgatatt atggagccct aatcagtttg attcaggtct
    23221 tgtaagcata ttgaaattaa atccgctgta ctatgttgtt caaggttatc gtgagtcatt
    23281 tttaagtgaa gcttggttct gggagcatgg aacatatact ttgtatttct gggttttcac
    23341 gttagtaatg ttcgtaatcg gctctgcagt gttccgtaga ttgaaaccac atttttcaga
    23401 tgtgctgtaa ggagaatagc tatgacagaa tatgcaatta gactaaaaga tatcacaaaa
    23461 tcttataata tgtacgcaaa accttcggat cgatttcgtg aagccctcaa tccttttaag
    23521 aaatcctatc atgatgtatt ttatgcgttg aaaaacgtga atgtagaaat aaaaaaaggt
    23581 gagatgatcg gtttcgtggg agaaaacgga tcgggaaaat ctacaatgtt gaaaatcatt
    23641 acgggagtac tgacaccaac atccggcacg atggaaatcg aaggaaaaat ctcagctttg
    23701 ctagaactag gatcaggagt caatcctgag tattctggct atgaaaatat ctatttaaat
    23761 ggaatggttc taggattttc aagagaagaa gtcgatgaaa tggtcgatga tatcattgaa
    23821 tttgctgaca tcggagacca tatttatcaa cctgtaaaaa cttattcaag cgggatgttc
    23881 gttcgtctag cttttgctgt agcaatcaat gtgaatcccg atattttgat tgtggatgaa
    23941 gcattggcag taggcgatct tgaatttcaa ctaaaatgta tggaaaaatt tacagaaatc
    24001 aagaactcgg gaaagacgat tttattcgtt tctcatgata tcagttcgat tcgtcgtttt
    24061 tgtgatcgtt cctattggtt gaaaaacgga gaagtcgtcg aatatggcga tacgatggaa
    24121 gtaacgacga attatgaaaa ctttttaaag aaaaaatcag ttaaaacagt ggatcggaat
    24181 aaaaaccaag tagaacggtt cgccgctcca gatatcgtcg acgtgacgaa agcggaattg
    24241 ttggataaag acaaaaatcc ggtggatatg ctggaacaag gagaaaaact atacgttaaa
    24301 gtaacttatg aagtaaaaga tgatacaatc aaaaatccgg ttttaggaat tgcgctacga
    24361 acagtcgaca atttttatgt atgcggactt aatactctac tagatggtat caagattcct
    24421 tggaaaaaag gagagaatat cttttatctg gagtatgaaa agttaggttt gttagctggt
    24481 gaatattatt ttgacgttgc ggtctttgaa gaaaatgcaa cggttccttt ggtatataag
    24541 acaaagtaca tgaacttgtt tgtcagcggt tcttatatcg gagaaggcat cgtggtctta
    24601 gaccataaat gggaagaagg gactcatagc aatgaaatat gattttgaaa tggacctaga
    24661 tgaacaaagc agtgtgggaa aaattgccgc acagatcaaa ccaggaagta aagttttaga
    24721 gtttggtccc ggaaacggcc gattaacgaa acatttaatc ggagcaaaac aatgtgaagt
    24781 gagcattgtt gagttagata aggaattgtt cgattttgtc agcgaatttg ctcaagatgg
    24841 attttatggt gatatcgaaa gttttgagtg ggcaaactat tatgctggac agacatttga
    24901 ttacgtcttg tttgcggatg ttttagaaca tttggtagat cctggaaaaa cattgaagaa
    24961 agtcagagaa tttttgaatg aagaaggaga gatcctgatt acattcccta acttggcgca
    25021 taattctgtc atgatcgatt tgttcaataa ccaattgccg tgggcttctt atggtctcct
    25081 agatgaaacc cataattctt tttatactca tgatggcttc caaaaagttt ttgaaaaagc
    25141 gggattattc attaatattg aagattattt gtacttagct gtaggtgata cagaattaaa
    25201 atcaacttat gaagaattgc cggaagctgt acgctatgat ttcaaaatgc gaccatttgg
    25261 tgaagtctac caatactttt tctcattgat gaaacatcca gtagcacaaa gttccattgc
    25321 agagccacaa aattctaatt atgtaaaagt gttagaagta acccagcaaa cgaaacaaga
    25381 tgaaacaatc caacaaatac cattcaataa tttcacaggg gaaaatgaga cactaagttt
    25441 ccccgtatca gaaacaacag aaagaatggt gtttcgcttc gcgcaacaac ctagctttat
    25501 cgaattcagc gcagaagctg ccggagaaaa actaacattt atcgattcga atgctgtagt
    25561 caaaacagta aatgattgtt atttgtttga tgggaaagaa ttaccagagt tcgttctaac
    25621 ggatatttct gggaaagaag tgactatcca ttgtcactat cgctttatcg gggaattgac
    25681 accaacaatg aaagaattgt tggaaacgat tcgtcccatg gctgaagtca caaagcagct
    25741 ttctgagaaa aatgacgaat tagaaagaga aaatcatcat cttcttgagg aaaatacaag
    25801 actagaccat acgttaaaaa caaccacaaa tcgctattgc acattgttgg aaagtgatga
    25861 atggacaata aaacatagac taggacgacg taagaaagaa acttcgaaaa aaatccaaga
    25921 aaaagaactt tctatatgta ttgacgagaa aatctgggat gcagaaacaa aaatattaaa
    25981 aatcatcgga tggggaatcg ctaattcgga tcgccagccc ttgtcctata agttatcagc
    26041 tgaccaatcg ccatttttcg aagcaattcc tgtttcacga gaagaagtca atcaagcaga
    26101 acagctcgca aaaggaacag aagcgggatt tgagttacga atcctttgcg agcaagaacg
    26161 ttcattctta gtggaagcag tagctcaaaa tggccaatca tggatcattg aaatgtaaat
    26221 aatgattaac ttataaagat aaggagtaaa cgaatcgtgc aaaaaaaaga aattgccgta
    26281 tttatcgatc aaatcactcg tgaacgggcc actggcgatt tgcttattgt gggctgggca
    26341 attgatgaag tgaccaaaga aatccctacg ataaaggtag aaaaagaaaa tgtgattgct
    26401 gaagcaacac atgtcgtaag attggatatc aatcatttat ataatcttga tgttaagacc
    26461 caaagcggtt ttaaggtacg tttatcaggg aaaatgagag gaaaagccat cttagacttc
    26521 caaacagcga gacatcaaaa tggcatagct gtgaaattaa acggtaaata cccttatgat
    26581 gacggcattg aatcttcatg ggaaagaaaa aaaagattac tgaaaaaagg gataaattat
    26641 gcaaggactc atggcgtaaa aaaagcgatc cgtcgggtca aattggaatt gaagccagga
    26701 tcgatcgact atgcagagtg gatcagtcgt catgagaaac cagatttgaa agagcaaagg
    26761 aaagaagcac aaggatttgc gtaccgtcca ttgatttcca tacttatgcc tgtctataac
    26821 gttgaaatca aatggctcga aaaatgcatt gattcggtac tggatcaaac gtatgatcat
    26881 tgggaactat gtatcagtga tgatgcttct actgatccag ctatccgaaa gtgtctggaa
    26941 tcctatcaag caaaagatga tagaatcaaa gtcgttttcc gaaaagaaaa tggacacatc
    27001 agtctggcaa ctaattctgc attggaaata gcagaaggag aatttatagc actcttagat
    27061 aatgatgatg aattaccgcc atttgcatta tatgaagtgg caaaagtgct aaacgtacat
    27121 ccagaactgg atgtgatcta cagtgacgaa gataaaatcg atgcagatgg caatcgcttt
    27181 gatccgcatt tcaaagcaga ctggtcgcct gatacattga tgggaaataa ttatatctct
    27241 cacttaggcg tatatcgaac aagcattgtt aaagaacttg gcggtttccg taaaggctat
    27301 gaaggttctc aagattatga tttggtcctt cgtgtgacag aacaaatccc tgcagatcat
    27361 atttaccaca ttgaccgagt cttgtatcat tggcggacga tcccaggatc aactgcaagt
    27421 aatggcgaag ccaaaagtta tatctatgat tccggagtga aagcattgac agatgccctg
    27481 tctagaagaa acatcaaagg aagcgtacgt cctggcagaa tttctggatt ttatgagatt
    27541 gcttatgacg tcttgcaaga ggatttggtc agtgtgatca ttccaacgaa gaatggctat
    27601 gaagatctaa aaacttgtgt ggattcgatc atcgaaaaaa cgtcttattc aaattatgaa
    27661 atcattattg cggataacgg aagtacagat ccaaaaatgc aagaattatt tgcagaatac
    27721 aagcatcagc tgaaagaccg ctttatcgta gaattgattg acattccatt caattattct
    27781 cgaattaaca atttagcagc agaaaaagca aacggcaaat actttttatt cttgaataac
    27841 gatacagaag tgattgagcc agattggatg acggtgatgg tttcttatgc acagtttgat
    27901 cgaatcggct gtgtaggagc aaaactgttt tatccagatg atacaacgca gcatgcagga
    27961 gtattattag gaatcggtgg tgttgcaggg catgcattga ataactatga tcgaacacat
    28021 tgtggttatt tcggccgatt ggtcattgac gtcaattact tagcagtcac tgcagcttgt
    28081 atgatggtca aggcagctga cttcaatgct gttaacgggt ttgatgaaac attagaagta
    28141 gcattcaacg atgttgactt gtgcttgaaa gtttatgaac tgggtcgtta caatgtctat
    28201 gcacatcaag cagaattgta ccatttcgaa tctaaatcaa gaggttatga agatactcca
    28261 gaaaaacaaa gacgttttgc tggtgaaatc aaaaaaatgc aagataaatg gccagcatat
    28321 attgcgcatg atccatttta caatgataat ttaacaaaag aaggaatcgg cgacttttcg
    28381 ttgagaccag attaaactat aataaaaagc tgttatgttg tgattcgctc gcacataacg
    28441 gctttttata gttttttgtc aaaaaaaggc agaaggtgcc gactcaaaaa aataagtcga
    28501 ggttgggaca gaagtgttta actccgagaa ataagaagaa attcacgaaa attgcttttc
    28561 aaatttttgt gaatttcggc ttatttaaga agaagttgct tctgctccca ccgtttattc
    28621 gtttttaaag aatgagacaa aagtgtttct tacttttgtc ccacgctctt attcatgctc
    28681 agagcgccaa tagatacaaa atcatagcca aagtttcacg aaccctagct gtcaaagaat
    28741 cgatatttta ttttaatata ggctatttta aaataaatgg tttaaggtat tcctctacaa
    28801 aagccactct atcaattatc atgggtttaa aataagaagt gatggagata accagattgt
    28861 tcttagcgtc aatataaaga acatttccac tattaccaat tgctgaatat atttcgttct
    28921 cagaatctaa tacccaccat aaatagccat atctcatatt ttgtaagtct tctaaagcac
    28981 tggttctagg tttgaacatc tcgtttatcc acatgggtgg tacgatttgt tttcctttat
    29041 acatcccttt atttaggcat aatacaccaa tttttgccag atcacttgcc gataaacaca
    29101 aaccatatcc tgccgtcccg atatttagtg gatcacaaaa ccagacattg gttttaggag
    29161 acttatccat tgtaaactgc ttatgttctt cagctgtttc agcataataa ttgacatggt
    29221 ttggtatccc tataggatca aatagatact tattggcaaa atcaacagtt gttagcttac
    29281 ttgtctgata caacaagcca gttaatatgt gtaaacagac agtttgatag ttgaattcgc
    29341 cggttatacc ttttcttcct cccaataaat ctagtgatgt ttttgtccaa tcttcacttg
    29401 aacagacttt tgtccatgga tcgcctttac atttgtaggg tgctttcatt gttagcaagt
    29461 gcttgattgt aatagcgtaa atagttttct ccccgcgttt cactttatac tctggaaaat
    29521 aatctaatac tttatcatcg atacttttga ttaatccttg ctctaaagca atgccaatca
    29581 gcaaagatac aatacttttc gttgctgaca tgatatgaca agtatcgtct tttttataac
    29641 cattccattc atctgtataa acttctaccc catccttata tgcgacaatt tgacatatat
    29701 ttggttgtcg aaaaccaata aaatcatgca actcttgttt attcataaga tattacctca
    29761 ctttcttcta taagcataat gtcattaaaa atcgatttca attttttcga ttcttcacgt
    29821 aatacaggta ttcttatgga ctagacagcg ctttgggaag atggattctc tgaaaaatat
    29881 aaaggttcac tttatgagtg gataaaaaaa gcagcaaata cttcgacttc tttaatttac
    29941 caacattagt gaaaatcgat tcatctggag atttgtaatt tagagtcctt gtcttgatta
    30001 gaggtgtata aagcttttta ttagacattt ttaaattcca ttacaatttg cttataaact
    30061 aggtttttaa aggtgactat ggtaagataa tagagatatt attttgtgat taggagtgta
    30121 tatatgaata aaaatggaga atggagtgct gcacgacgga tattcattat attaatggat
    30181 gttattgtat acaatgcagc aatatatagt agtttcttac tgaagtttcg tggagagatc
    30241 ccattacgaa atttagaaac tttccaacac tcagcaatta ttatttcagt gatttttatt
    30301 gcgttgaaca ttttgttggg tgcatatgtt ttttataatc gaatgatcag tgacatcgtg
    30361 tttgtcacag tcatcggtca ggtattgatg acactaggga tcatggtagt gacttttgcg
    30421 ggccgctggt ttgcttttcc acgtcttgtt atcttacttt catttgttct cggtacgctt
    30481 ttcttaagta tctatcgtat tctcgtatat aaactttatt tacgcgtaag tcgagataaa
    30541 aaggtcgtca ttattgggtt agaaaaggat gtcgcaccag caatccaaaa ttttggttca
    30601 aagaaaaaca acaaacataa agtcgaagca gtcattcttg ataactatta tgcaaacatc
    30661 aaaaatatga ttaaagacat cgatattgtc tatcttgctt ctcatatcga agaaactgaa
    30721 aaaatcaaaa tctatcaatt agttactaaa aatgaaaaaa aattgttttt gaatacaaca
    30781 tttgaaaact tgacattgat caatccaaat attatgaatt tcgaagatga aagtattatt
    30841 gaagcatctg gatttagaat ccaacctgaa tatgaactat tcaaacgttt gtttgatatc
    30901 atcgtttcac ttgttttatt agttattgct tcaccattta tgttattcac agcaatttta
    30961 gtcaaagtga catcaccggg accaatcatc tataaacaag tgcggatcac aaaaaatcaa
    31021 aaggaatttt caatctataa attccgctcg atgaccgcca cagccgaagc aaaatctgga
    31081 ccagttttag ctaagagcaa tgatgcacga gtaacgcctg taggtaaatt tattcgagct
    31141 gtccgctttg atgaattacc acaaatcttc aatgtgctga aaggcgatat gtcgatcgta
    31201 ggaccacgtc ctgaacgtcc tttcttcgtc gatcagttca atgcagaaaa cccttattac
    31261 tacttacggc ataatgttcg tgctggtatc actgggtatg cacaagtcta tggaaaatat
    31321 gtctctgatt acaacagtaa attacgtttt gatttacttt atatcaaaaa atattcattg
    31381 atgcttgatt taaagatctt gctgcaaacc atcaagatcc tatttgataa aatgtcctct
    31441 caaggacttg aagaagacga aaatgtgatc tcattggcag aaattgcttt tccgaatgaa
    31501 aaaatctttc gctagttggg aggcgtaaaa atgaacaatc cgaaattcag cataatcgta
    31561 ccagtataca atgcagaagc aactattggt cgaacgattg atattctaaa agaattggat
    31621 tatgaagagt acgaagtcgt tttagtaaat gatggatcaa cagatcaaac acaggaaatc
    31681 attcaagaac tgattgaaaa tgatgaacgt tttgaattga ttactacgac caatcaaggg
    31741 cccggattag caagaaacga aggaataaga cagtcaaaag gagaatatct cttattcttc
    31801 gatgcagatg acacgccagt aaaaacgatc ttgtcagatt atgatcattt gctagaaaag
    31861 agtccaaaag cggatcttgt tgttagttcg tttgtttttc gtacaatgga cgagggaaga
    31921 atcgtttccg aaaaagaaaa cttagttaca gaatgtcact atctaactca cgaagagttt
    31981 ataaaagaca tgtatcagtt aatgaatgaa cagttgatgt atgttgtatg gaacaaatgc
    32041 tatcgtagcg atattctgaa aaataaccaa attctattta aaacttataa tagttgtgag
    32101 gatcgaatat ttaacttaca ttattatgaa tactgtcatc aagtgatcat gaacccaaaa
    32161 gtagaatata tttatgaatt tgaaggtggg aaggggatca ctaatcaata ccgacctaac
    32221 aagttcgaaa catttaaaga attctattca ttagcaaata ctgtaacaga cgatattaat
    32281 aaagacggta tggcggcgct tcttttaaaa gggacaactt cagttatttt ttctatttat
    32341 agtacctctg caagaacttc aaaagagaag aaagacgaag caaagcagat attgagtgat
    32401 ccagtgatcc gagaagccaa gcaaattgct aagacggatt cttcagcaaa aaaaataaca
    32461 aagttgcttt acaatatgcc agctccacta tttttatctg ctgtaaaagt tggaagcttc
    32521 gttgaagtga aaatgccagg gttaatggct gctttgaagc gagtatatta gttaatggag
    32581 gaaaatataa tgaaaagaat tttgcatttt cagggacgaa tgggtttagg tggagcagag
    32641 tctttcatga tgaatttata tcgtaaaatt gatcgtacaa actatcaatt tgattttttg
    32701 atttatgaag attatgcaga tgttcaagac tatcattcag agattgaaag attaggaggc
    32761 cgtatttttg ttgtacctaa tcctaagaaa aatattttta aatatttaat agaagtgaat
    32821 aagttactaa aaaaagaatc tttttctata gttcacaatc aggtttattt tggcggggga
    32881 attaatttat ggttagccaa aaagaatgga atcagacaga gaattgctca tagccatgca
    32941 actgaagatg gaaaatctca aaatattgta atgaatgtat tgcgtaaatt tttaaccaaa
    33001 ttgttgttac aaaatgcaac agactacctt gccgttagtc aagaagctgg tgaaagtctg
    33061 tttcaaaacc acccctttga gatagttcat aatggtattg atttagagct ttatagtaaa
    33121 aattcagaag ctaaggttaa taagagaaaa gaattggata tctcaatgag tacttttgtt
    33181 gtgggaaaca ttggacgatt agagaaacaa aaaaatcaat cttatttgtt agagattttt
    33241 ttagaaatac taaaaatgaa ttctgattct tacttgattt tagtaggtga agggtcactt
    33301 agaaataaac tgaaagccaa agcagtagaa ttggatatag agaaaagagt aaaattttta
    33361 ggagaaagaa acgatattcc cgagttatta tctgtgatgg atgtttttgt aatgccttct
    33421 ctttatgaag gtctgccaat ggttggatta gaagcacaag cagcaaaatt aaaattagtc
    33481 ttagcagata ctatttccaa agatacaaag ttaacaaata atgtggattt tgtatcatta
    33541 gatgaaacac cacaagaatg ggcaaaaaaa gtaataaaag agccgtatgc taataaaata
    33601 acacaagaac taaagagtta tgatgtttcg tacactactg aacaaatgga gagtatatat
    33661 agtagagatc attaaaaatg ttcaagatat tttaggagtg attatttttg aaaatatgtt
    33721 atttgatatt ggcatacaat gatattggca acttaaagag attggtcaat cgattgaacg
    33781 aaaatgcagt ttttattatt catatagata aaaaaactga tatagaacca tttattgaag
    33841 catttaaatc atatgataat gttgaatttt tatgtaataa aaaccgtgtt aaaatttatt
    33901 ggggtggttt ttccattgta caagcagaaa tcaatttagt aaagagagca ttacaaaatg
    33961 agaaata
//
LOCUS       DADVXU010000016        33878 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.017, whole genome shotgun sequence.
ACCESSION   DADVXU010000016 DADVXU010000000
VERSION     DADVXU010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 33878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.017"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            111..1076
                     /locus_tag="KB953_000792"
     CDS             111..1076
                     /locus_tag="KB953_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBB6865788.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(1133..1363)
                     /locus_tag="KB953_000793"
     CDS             complement(1133..1363)
                     /locus_tag="KB953_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HBB6865789.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(1449..2336)
                     /locus_tag="KB953_000794"
     CDS             complement(1449..2336)
                     /locus_tag="KB953_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBB6865790.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(2425..3648)
                     /locus_tag="KB953_000795"
     CDS             complement(2425..3648)
                     /locus_tag="KB953_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBB6865791.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(3665..4711)
                     /locus_tag="KB953_000796"
     CDS             complement(3665..4711)
                     /locus_tag="KB953_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBB6865792.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIRSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(4875..6602)
                     /gene="ptsP"
                     /locus_tag="KB953_000797"
     CDS             complement(4875..6602)
                     /gene="ptsP"
                     /locus_tag="KB953_000797"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBB6865793.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(6602..6868)
                     /locus_tag="KB953_000798"
     CDS             complement(6602..6868)
                     /locus_tag="KB953_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HBB6865794.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            7347..9581
                     /locus_tag="KB953_000799"
     CDS             7347..9581
                     /locus_tag="KB953_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBB6865795.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            9729..10049
                     /locus_tag="KB953_000800"
     CDS             9729..10049
                     /locus_tag="KB953_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HBB6865796.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(10254..10478)
                     /locus_tag="KB953_000801"
     CDS             complement(10254..10478)
                     /locus_tag="KB953_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HBB6865797.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(10676..12256)
                     /locus_tag="KB953_000802"
     CDS             complement(10676..12256)
                     /locus_tag="KB953_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HBB6865798.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(12657..14033)
                     /locus_tag="KB953_000803"
     CDS             complement(12657..14033)
                     /locus_tag="KB953_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBB6865799.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(14161..15279)
                     /locus_tag="KB953_000804"
     CDS             complement(14161..15279)
                     /locus_tag="KB953_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBB6865800.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            15421..15855
                     /locus_tag="KB953_000805"
     CDS             15421..15855
                     /locus_tag="KB953_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB6865801.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(15933..16466)
                     /locus_tag="KB953_000806"
     CDS             complement(15933..16466)
                     /locus_tag="KB953_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HBB6865802.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(16560..17738)
                     /gene="mutY"
                     /locus_tag="KB953_000807"
     CDS             complement(16560..17738)
                     /gene="mutY"
                     /locus_tag="KB953_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HBB6865803.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(17731..18528)
                     /gene="recX"
                     /locus_tag="KB953_000808"
     CDS             complement(17731..18528)
                     /gene="recX"
                     /locus_tag="KB953_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBB6865804.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            18635..20017
                     /gene="rlmD"
                     /locus_tag="KB953_000809"
     CDS             18635..20017
                     /gene="rlmD"
                     /locus_tag="KB953_000809"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBB6865805.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(20191..20769)
                     /locus_tag="KB953_000810"
     CDS             complement(20191..20769)
                     /locus_tag="KB953_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HBB6865806.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            21091..21561
                     /locus_tag="KB953_000811"
     CDS             21091..21561
                     /locus_tag="KB953_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HBB6865807.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(21669..22895)
                     /locus_tag="KB953_000812"
     CDS             complement(21669..22895)
                     /locus_tag="KB953_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBB6865808.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(23649..23849)
                     /locus_tag="KB953_000813"
     CDS             complement(23649..23849)
                     /locus_tag="KB953_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBB6865809.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(23983..24591)
                     /locus_tag="KB953_000814"
     CDS             complement(23983..24591)
                     /locus_tag="KB953_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HBB6865810.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(24676..25146)
                     /locus_tag="KB953_000815"
     CDS             complement(24676..25146)
                     /locus_tag="KB953_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBB6865811.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(25194..26108)
                     /locus_tag="KB953_000816"
     CDS             complement(25194..26108)
                     /locus_tag="KB953_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBB6865812.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            26254..27057
                     /locus_tag="KB953_000817"
     CDS             26254..27057
                     /locus_tag="KB953_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HBB6865813.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            27235..28182
                     /locus_tag="KB953_000818"
     CDS             27235..28182
                     /locus_tag="KB953_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBB6865814.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(28250..28543)
                     /locus_tag="KB953_000819"
     CDS             complement(28250..28543)
                     /locus_tag="KB953_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HBB6865815.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYNPNLEEPTYHFKKQ"
     gene            complement(28571..29236)
                     /locus_tag="KB953_000820"
     CDS             complement(28571..29236)
                     /locus_tag="KB953_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HBB6865816.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(29376..30008)
                     /locus_tag="KB953_000821"
     CDS             complement(29376..30008)
                     /locus_tag="KB953_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBB6865817.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(30015..31082)
                     /locus_tag="KB953_000822"
     CDS             complement(30015..31082)
                     /locus_tag="KB953_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBB6865818.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(31079..31810)
                     /locus_tag="KB953_000823"
     CDS             complement(31079..31810)
                     /locus_tag="KB953_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HBB6865819.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(31979..32458)
                     /gene="greA"
                     /locus_tag="KB953_000824"
     CDS             complement(31979..32458)
                     /gene="greA"
                     /locus_tag="KB953_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBB6865820.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(32594..33769)
                     /gene="mltG"
                     /locus_tag="KB953_000825"
     CDS             complement(32594..33769)
                     /gene="mltG"
                     /locus_tag="KB953_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HBB6865821.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
BASE COUNT        10113 a         6866 c         5613 g        11286 t
ORIGIN      
        1 tttgattatc aaagtatttt ctgtgaacag aactatagaa ttccctaaaa cttgtccatt
       61 atttcaaaat ttgttatagt ctttttattc aaagcgtaaa aggagatcct atgttaaaag
      121 aaaaaatgaa aactctcccc atgctgccgg gtgtctatct tatgaaagac cgagatggta
      181 aggtcatcta tgttggtaaa gcaaaacggc tcaaaaatag agtaagcagc tattttcatc
      241 agaataaaca gcattcaaaa aaagtattac gtatgattca tcatataaca gattttgatt
      301 tcgtcgtagt agacacggaa ttagatgctt tattgctcga gtgtcaattg atccaacatt
      361 accgtccttt ttataaccga caaatgaatt atttcagtaa ttataattat gtacatatta
      421 ccaataaggg atttgtatta acagatactc ctacggctcg aacatacggg ccttttcgtc
      481 tctataaaaa aatgccgtct attttgcgta taatggaaga aacttatcaa atgccttggt
      541 tgtctgaaat tagtttgtta gctttgcgag tccagcttcc tgacctacag gaaatgactt
      601 ttgaacagaa aaaaaaggaa ctccaagggc tgtttcaagg ccgaaataaa aagctactga
      661 cttacttgaa aaaaagacaa caacatttca tctatcagtt gaatttcgaa aaagccggta
      721 tgcttcaaaa agatatcgaa ttagttactt attttattag acggatccag gaacaaaagc
      781 agtttttaag aacaccatcg ctcaccttca gcatgccatt agcagcagat gagagtcaga
      841 aaaagcacta cctcgtttgt tatggtcaac ttgcagaaac aatgattacc tctggtgacg
      901 tttctcctga cttctattat gaaaagaaag aagctcattt atctttgaaa cgtcaattgt
      961 caaaagaaga aatcgatcct gtacaaatct tgatcagtta ccgcaaaaaa ttagaaaaag
     1021 aacagtttga aattgaactg ttaaacaaaa aagaagctga aaagcaacta aattagttgc
     1081 ttctcggctt cttttaggca tcttcaactt caaatgtcaa ttatcctaaa atttattgga
     1141 ttaagtcata gatttccatc gctacgatat cgatattatc aaattcgtat tgctgatgag
     1201 tatctgtttc agttaattca aatgtatcgg tcgctttatc ataagttact gtacacttct
     1261 ctacgccttc tttttcaaaa cggcgaactt gcagttcatt atctgctgac tcgaccatag
     1321 cctccagtcg ttttatgatt gcgactaatt gtgaatgtgt catcggaggt cctccttttc
     1381 ttatttctgt tatattgtat caaaagcata gcctacttgc attgattttc tctaaaaaaa
     1441 ctcgtttttt atgatttttt ttctggtatt tttccttcag gccaccaaag tttgatcaaa
     1501 gcttgggttc cgtcattctc aaaaccttta tctgccaact cttcatatag tttcaatgct
     1561 tgggtagttg ctggcaatac aagatccatt ttttctgctt cttctaatgc aatctttaaa
     1621 tctttgataa aatgtttgac aaaaaaacca ggtgtgtaat cttcttttaa aatacgagga
     1681 gaataattgc ttaaagacca gttagctgca cttccacctg aaagcgtctc aatcactttc
     1741 tttaaatcaa gtccgttttt ttgtgcatag acaagcattt ccgtcattcc tgtcatcgta
     1801 cctgcaatca tgatctgatt agccattttt gtatgctgac ctttcccagc agaaccatgc
     1861 aatgtaaatg tcgtaccaaa ttctttgaat aaaggcagta cttgatcata aacggcttct
     1921 tctccaccga ccatgatcgt cagtgtacca ttctttgctc ctaagtcacc accggatacc
     1981 ggtgcatcta acgcatctgc gcctttttct tttgctgttt ttgcaatttt ttctgccagg
     2041 cttggtgtac ttgtagtcaa atcaactaaa atatgtcctg agatatctgc agaaaaaatc
     2101 cccgactgtc catagtagat ttcttctaca tctttggggt aacctaccat tgtaaagaga
     2161 atattacttt gttctgctac cgcttttggt gtatctgccc agatagctcc ttctgttact
     2221 aaaggatcag cttttgcttt tgtccgatta tagacagcta cttcgtgtcc tgcttctagt
     2281 agatggcgtg cgacagcact tcccatgacg cctgttccga taaaaccaag tttcattttt
     2341 tcgacctcct aaaaatcaaa aatccttgct tctatgctag tatacgctag aaacaaggat
     2401 tttttcataa aaaatgtttg aattttatct tttaaatgaa tttaatttta ttttgattcg
     2461 ttcaatcgag tcttcttctt cttttcttcg catctcttct tcatgataaa caaccatgtc
     2521 ttgataaaat tcgatcatag cttcgccgaa atgtgtagat gaaatatcat aaagtttagc
     2581 ttctaaaact tttggatctt taggaacttc actggaaata taatcaataa gtgctggtgc
     2641 aaagtcttcg tcggtttcaa aagttttccc taatgaagga tgatcgaaca atcggttcaa
     2701 ataagcattt ccttccacga cacaaggtgt tccggctgcc atcgcttctg tataagtaag
     2761 cccttgtgtt tctgaagtag aagcactgac aaaataatct gccgctttat aataaagtgc
     2821 tacatcattg ttgtccactt ctcctgtgaa ctgcacaaat tcttctaact gcagttctga
     2881 gatcaattcc tctaactctt ctacataagg tccttttcct acgataacta atctggcgtt
     2941 aggataagcg gagatgacct gaggaaaccc attaatcaag gcttggatat ttttctcata
     3001 agaaatcctg cttaaagaaa gaatcatcaa atggttttcc tgaagaccta gtgactccct
     3061 taatttgctg atatcctgct gagtgatatc agaacgtttg aatttttcaa tgtcgatacc
     3121 agtaggaatc acgcgaagcg gtgctattac accatagctg cgtaatgtat caataacacg
     3181 ttcacttgga caaactacac ctgtcgtatg atttgcaaat aatctagaca agtattttac
     3241 atgggattgc ctgatcactt ttcctttacc gatataatga aggtagtctt catacatcgt
     3301 atggtaagta tggatcagag gtattttcaa cttcttagct accatcttcc caagtatccc
     3361 tgctccgaat tctgtatggg tatgaataag atctagttct agttctttgg caaccaaata
     3421 agcataccac atgcctctaa caacaactct tcgatccttg aaagaaataa atggtacact
     3481 tggcattcga atcacatctt tttccaactt atccgcatta ggatcggtcg ttgtaaaaat
     3541 atagacttca tgcccatgtc tttctaattc ttgttttaat gttttgatcg atgtagcaac
     3601 tccgctgact tgcggaaagt aggtatcggt aaaaaagccg attttcatgt ttttcgcctc
     3661 caaactagtc cttgacctga tttacttttt ttaatttgct agagactttt tcattcagta
     3721 cactttcgta tacattttta agttcttcac cgattttatt gatacttcgc tcttttgctg
     3781 tttgataacc ggcttgtgtc gtactcggta gttcttgatt ggtaatctgt tggatcagtc
     3841 ggataaattc ttcgtttgtc ttccccatat agcagttctt gctatcttct aaccagccat
     3901 tatacactgg aatatctcga acaagtacgt tttgtttgct tgctaaagct tctaaaacaa
     3961 cgatcccttc tgtctcttca taagaaggga aaaagaatag atctgcacct gaatacgcac
     4021 cttctatgat ctctccacga atgtaaccag gaaacaggac attttcagga tgatcttctt
     4081 ttacaatttt ccggatgttc cgaggaatcg agtacatggg aacatgacca aaccaaataa
     4141 atgtgtattc gggcatcttc ttagcaattt ctacgaaatc aatgattcct ttacgttcga
     4201 aaaacaaacc gacacaaatg attacttttt gatccggaga cagtttgaaa tattctctga
     4261 acttttgttc tttcattgga tcagggtgat atttctctaa gtcgatccca ttggaaatag
     4321 cttgaatcgg cacacggata ccatagcttt ccaacaactg ttttgaataa ggtgttggcg
     4381 tgatcagata gtccgctttt tggtacaatc ctaccagata tttttttacg ataggagaaa
     4441 gctggttcga tctgataaag gaatttcgga aatcttcctc tgtagaatgc gcatgataga
     4501 caactttttt tccttctctt ctggctttat tgaccatatt atgactattg attccgtatg
     4561 tattgatgtg caggatgtca taatcttctt tttcatctag tgtataagag ataccgactt
     4621 ccgtcagagc acgtttttga tgatcaagcg cacgcccgat tccagatttt gcaagcatct
     4681 tttcactttc aaaatataat aataccttca ccttacgtcc cccttcactt atatagtata
     4741 acatagccta caaactgtca ctcaaactta agccctattt ttgtctattc ttactgacaa
     4801 aaacgttttc ttaaaaaaga acaaaaaaac tggcgtagct cttttacaag ccacaccagc
     4861 ttttgatacg ttgtttattt tacatattca tgaacaagtt caacgacttc ttccatcata
     4921 tcgcattctt tcaatgcacg atcagcaagt tcagccattt tcgctgtgtc caagcgtttc
     4981 attaaactac gcgttttaag aatagatgtc gcactcattg agaactcatc tagacccata
     5041 cctactagca atggaacagc tgtttgatct ccagccattt ctccacacat acctgcccat
     5101 tttccttcag cgtgtgctgc atcaattaca tttttgatca aacgtaaaat tgatgggtta
     5161 tatggttggt acaagtaaga aacgcgttcg ttcatacggt ctgctgccat tgtgtattga
     5221 atcaaatcgt tcgtacctac agagaagaaa tctacttctt ttgcaaactt gtctgctaag
     5281 actgctgctg ctggaatctc gatcatgatg ccgacttgga tcgtatcaga tacttccttg
     5341 ccttcactta tcaatttttg tttttcttct tcaaagatcg cttttgctgc gcggaattct
     5401 ttgagtgtag ctaccatagg gaacatgata cgtaaattac catgaacaga tgcacgaagc
     5461 aatgcacgca tttgtgtacg gaacatttcg tcgccttgtt cagataaact gatacgcaat
     5521 gcacgatatc ctaagaatgg attcatttca tgaggtaatt gcagatatgg tagttcttta
     5581 tctccaccga tgtccattgt acgaacaact acaggttttc cttccatacc ttctaataca
     5641 gctttgtaag cttcatattg gtcatcttct gttgggaagt ctggagaatc catatacagg
     5701 aattcagtac ggtacagacc aactgcttct ccaccattat tatggacacc aactagatct
     5761 tttggtgttc cgatgtttgc agctaattca aaatgcttgc cgtcagctgt gacagtttct
     5821 gcatttttta atttttccca ttctgctttt tgtgctgcgt aatctgcacc tgctttttca
     5881 aattcagctt tttgttcatc tgtaggatcg atgattacat cgccttcgat cccgttgaca
     5941 gccaaaatat cgccttcttt gactttagca gtaatttctt ttgtcccaac gattgctgga
     6001 atttctaaag aacgtgccat gatagcagaa tgtgaagtac gtccaccaat gtctgttaca
     6061 aacgctttta caaaattgcg atctaattgt gctgtatcac ttggagtcaa gtcatgtgca
     6121 acaacgacca cttcttcgtt gatcattgat ggatttggta aagtaacgcc taataagtgc
     6181 gctaaaatac gtttagcaac gtcacgaata tctgcagcac gctcttgcat ataagcatta
     6241 tcttccattg cttcgaacat accgatatac atatccgtta cttcttttag cgctgattca
     6301 gcatttacgc tgttgtcttt aatattttgc ttgatttgac ctaccatttc tggatctgaa
     6361 agaaccatca aatgggcatc aaatacttgc gcttctgctt cacctaagct ttgcgctgct
     6421 ttttcacgaa tttgctgcaa ttcttcagta gatttagcta atgcagcatc caaacgagct
     6481 tcttcagccg cagtatcttc tactgttgcc ttactgaatg ttaaatccgg ttgaactagc
     6541 aggtaagctt ttgcaacggc tacaccgtca ctagcggcaa tcccttttag catttcaacc
     6601 attattcaga taagccttct ttcttcattg tttcaacgat tgctgccatt gcatcagctt
     6661 cgtcagcacc ttctgcagta atagtaacat cagaaccttg acctacacct aaagacataa
     6721 cgcccatgat tgatttcaag tttactgatt tacctttata ctctaagttt acatcagagt
     6781 taaatttgct tgctgtttgt actaatagtg tagctggacg agcgtggatc ccagtttctg
     6841 ctactacgtg aaattctttc ttttccatat taatcggtct cctttaagtg agtgttgttt
     6901 ttatgtgagg gttttctttt ttagtaattc agagaatcct aacaaatgcc accctttcat
     6961 ctgtaaagat taacattttt tttcgttaga tacaacagtt ttcattcaat ttcagataaa
     7021 agtattcaaa ttattccgga ttctttttca tccttttctt cacattttat aacaaacgtg
     7081 tttatttcct ttttcttgtc gctttctctt ctcctgacgt ttatcattct ttcctttgac
     7141 cctctttgtt ttcatatgaa acgaattact tgcttccctt tttattttta gtataataag
     7201 agtcaaaggt caggaatggt caatagagat ttcacctttt ctacctcata aatctcttaa
     7261 aaaactaggg gttattcttt tacctcatga aaaacgagat gtaaaatatg gatgtaccca
     7321 tttttgtgaa aggacgtgaa tgattcatgc tttgtcaaaa ctgcggcaag aatgaggcaa
     7381 cgattcactt gtatgccact gtcaatggtc agcggacaca actcgattac tgtcagagct
     7441 gttatcaaaa aatcaaaaat caacaaaatg gaggtctgac taatatggcc caaaatgatc
     7501 catttggatt tggcagtttg gacgatcttt tccgttccat gtctcgccaa atgcaacagc
     7561 aaggaaatta tgagcaaaca ccaccaacac aatttggcgg aaacaacaac ttcaatggtg
     7621 gtcagccacc tcaaggcaat acagaaggtt tattgggaga atacggaatc aatatcacag
     7681 aacaagcacg acaaggtgat atcgatcctg tgatcggacg cgatgacgaa atcaaacgag
     7741 tcattgaaat tttaaatcgc cgaaccaaga acaatcctgt cttgattgga gaaccaggtg
     7801 ttggtaaaac agcagttgtt gaaggtttag cacaaaaaat cgtagatggt gatgtaccgc
     7861 aaaaattaat ggacaaagaa gtcattcgtt tagatgtcgt atctttagtc caaggtactg
     7921 gtatacgagg acaatttgaa gaacgtatgc aaaaattgat tgaagagatc cgtcaagcag
     7981 aaaatgtgat cctattcata gatgaagttc acgaaatcgt cggtgctggt tctgctggtg
     8041 atggaaatat ggatgctggt aatattttaa aaccagcttt agctcgagga gaattgcaaa
     8101 tggttggtgc tactacctta aatgagtacc gcatcatcga aaaagacgct gctttagaac
     8161 gtcgtatgca acctgtacgg gtagatgaac caactgtcga agaaacgatc agtattttga
     8221 aaggtttgca aaaacgttat gaagactatc atcatgtaaa atatactgat gaggccatcg
     8281 aagcagccgc aactttatcc aatcgttacg tccaagatcg cttcttgccg gataaagcca
     8341 tcgatttgct agatgaaaca ggttctaaga aaaacttgac gatccaaatc gttgatccta
     8401 aaacaatcga gaaaaaactg caagaagcag aagaacaaaa agttttagct tctcgagaag
     8461 aagatttcga aaaagcagct tattatcgcg atcagatcaa taaacttcaa aaaatgaaag
     8521 aacgtcagct cactgaagag gaaacacctg tgattaccga aaaagatatg gaaaaaatcg
     8581 ttgaacaacg gacaggtatt ccagtgggtg aattaaaaga aaaagaacag acacaactga
     8641 aaaacttagc agatgacttg aaagctcatg tcatcggaca agataacgct gtagatcgag
     8701 tagcgaaagc gattcgccga aaccgtgttg gattaaacaa acagaatcgt ccaatcggct
     8761 ccttcttatt tgttggacca actggtgtag gtaaaacaga gcttgcgaaa caacttgcct
     8821 acgaactatt cggttcacaa gattcaatga ttcgtttcga tatgtctgaa tatatggaaa
     8881 aacatagtgt ctctaaattg attggttctc ctccaggtta tgtcggatac gaagaagctg
     8941 gtcaattaac agaaaaagta cggagaaatc cttacagtct tgtcttgtta gatgaagtag
     9001 aaaaagctca tccagatgtt ttgcatatgt tcttgcaaat cttagatgat ggtcgattga
     9061 cagatgcaca aggaagaacg gtcagcttta aagatacgat catcattatg acaagtaatg
     9121 ccggaactgg taaagtagaa gcaaacgttg gttttggtgc ggcacgcgaa ggtgttactc
     9181 gttctgtatt gaaccaatta aacaactact ttacaccaga atttctgaac cgttttgacg
     9241 ggatcatcga attcagtgca ttatcaaaag aaaacttgat gacaatcgtc acactaatgc
     9301 tagacgatgt caaccaaatg ctggcagcac agcaacttca catcgaagtt cctacgaatg
     9361 tcaaagaaaa attagttgac ttaggctatg atccatctat gggtgctcgt cctcttcgac
     9421 gcacgatcca agaacaaatc gaagatggta tcgctgaatt ttatcttgat cacccttcta
     9481 tccatgagtt gaaagcaaaa ctggataaag atggaaaaat catcgttact tctaaaccag
     9541 aacgtttggc taaagaatca gccgaagaaa cggctgaata aatccagttt tatagcgtga
     9601 gacggtattt ctttgagaaa tgccgtctca ttcttttttc agaaaagtca aaatactgtt
     9661 cattttataa aaaagaaatt ataataagag aaacaagcaa ttaaaaatac ataaaagagg
     9721 tgagcgccat gaaattggta aatgtaacaa acagccattc ccgtcttgta ttaaaccagc
     9781 ttgaaaacac tgatgcccat ctagtcaagg tctacacagc cggaaatacc actattgtct
     9841 acactgaagc ccctgaacat aatgaaattc tgctgatcaa tgacaaacgt aaaatccagc
     9901 cgaaagaaat cgaagatgct aaaaattatt tcttagataa aatcaaagac gccgtttacc
     9961 acgaggatga aatcaaagta atcgaattgg caggccttgt tgaaatctcg attccaaaac
    10021 atgaattagg cgctacatta gccgtgtaaa caaattttga acataataat taaagagcgt
    10081 gggacaaaag taaagaacac ttttgtctca cgctctaaac acgtataaac ggcgaaagca
    10141 gaagcaactc cttcggaaat aagctgaaat tcacgaaaat tgcttttcaa atttttgtga
    10201 attccagctt atttcttgga gttaaacgct tctgtctcag cctctttttc tttctagctt
    10261 tttgattttt ctcttttctt ttgtactttt ctcttttcat tttcagttct acgcgtgtct
    10321 ttatttttaa ctttttcctc tttatggatg attgatcgta ctttttgtgc aatttgctcg
    10381 tttttgtccc agtcttttac atctaataat cgacataatc gtaacatctc ttcttttttc
    10441 tgtttttttc tttcacgtct ttgccattct gtttccatgt gtcacactct cctttcttta
    10501 atcatatgta cgcaaaataa tttttatatt tttttgatta gacatttcta tgattttaat
    10561 atagtaaaaa gagccgtcac ggaaaatccg tcacggctct aaacaaaata tgagaggctg
    10621 tttttgtttg gctattctta ttacttgatg cagggcagct tgtgtcaaga cttctttaca
    10681 tcaaactttt taattttaca ttgggatatt tatccgcaaa ccagcgttct gcaaattggt
    10741 tctcgaataa gaacaacggt tggtcgaaac ggtcacgagc caagatattg cgacttgaac
    10801 tcatcttctc atccaaatct tctggatcga tccagcgagc gattttgtgt cccattggag
    10861 acatgattac ttcggcgtta tactcattta acatacgata ttggaatact tcgaattgta
    10921 gctgaccaac agctccaatg atatattctt ccgttagata tgttttataa agctgaatcg
    10981 ctccttcttg aactaattga tagattcctt tgtggaagga tttttgtttc atgacatttt
    11041 ttgcagtcac tttcataaac agctctggag taaatgaagg gagttcttca taagcaactt
    11101 ttagttttcc ttcgaacaat gtatccccaa tctgataatt gcctgtatca tatacaccga
    11161 tgatatcgcc tgctactgct ttttcgacgt tttctctcga atctgccata aattgtgtga
    11221 cattgcttaa tttcagtttt ttatttgtac gttccaacca gacatccatt cctcgttcga
    11281 atgttccaga acagattcgg acaaaagcga ttcggtcacg atgggctgga ttcatatttg
    11341 cttggatctt gaatacgaaa cctgaaaatt cttcctcata aggacttacc atctgatcat
    11401 cttgtgtttt gtgtccataa ggagctggtg ccaattcaac gaaagtctct aaaaaagtct
    11461 gtacaccgaa gttagtcaat gcagaaccaa aaaatactgg tgtctgatcg ccacgagcaa
    11521 ttttttcttg attaaatgca tctccagcct ctttgattag ttctacttct tctaagactt
    11581 gttcatataa ggaattttca gttagaggat gattttcggg aagcagtcca tcttcatcca
    11641 attctactaa tcgttcatcc tgataatttt ccggacgata aaactctaca cgattatggt
    11701 agatatcata aagaccttct agccctttac ccataccgat tggccaattc atcggataag
    11761 attcgatatc taatagctct tctaattctt ctaacaattc caatggttca cgaccatcac
    11821 gatctagttt gttgataaaa gtaaatatcg ggatgccacg tttttttaca acttggaata
    11881 atttctttgt ctgtgcttcg atcccttttg cactatcgat taccatgacc gcactatcca
    11941 cggccatcaa agtacgataa gtatcttcag aaaaatcttc atgtcctggt gtatccaaga
    12001 tatttactcg tttcccttga taatcaaact gcataacaga actagtaaca gaaatcccac
    12061 gttgtttctc gatttccatc cagtctgatt tggcaaaatt acctgttttt ttgcctttta
    12121 cggtcccggc ttgacggata gccccaccaa acaatagtaa ttgttctgta atcgttgttt
    12181 tcccagcatc cggatgggaa atgatcgcaa aagttctgcg attgtccact tgttgcttta
    12241 attttggatt attcataacc taactccatt tcttccattc aattatttat attttctcta
    12301 ggcacagatt gtccgatctc ttaaagaaag acaatttcat atagggctgt gacaaaagcc
    12361 gtgtagcagt ctctttttcc gagtaaacgg cgttctagag acagcctatc agcaataagc
    12421 ccacccaagc caaaaatacg aaaagctgtt tttgcttggc tgctcttatt acttaatgca
    12481 ggatagcttt ttcacaacct ttgtcaacta ctacaactga atcagtatag catggattca
    12541 gcgaatgaaa aagccaccaa accataagag tcaaatattt ttttgtcagc tgactaaaag
    12601 aaaagacgtg ttgcttgttt attcaagcaa cacgtctttc ataaccaagt ttccaattat
    12661 cttcttggct cgtcatcttc gaattctttt ctgaagtatc ttcgttcttc ttcaggttcc
    12721 gcttctgttt cttccggatc atgaaaaatg acacggattt taaggattcg agaaccttcc
    12781 atttcttcag aaaccagcgt aatattatcg acatcaaacg ataacttttc gccttcatcc
    12841 ggaatcatgc ctaatgctgt aatcaaataa ccagccatcg tatctacatc actcatatgc
    12901 agatcactgt caaaagcttc attgaactca tcaatcagca tacgtccttg gatgatatat
    12961 tcgtgttcat cgattttttc ataaagattc tcaacttcat ctgattcatc atcgatttct
    13021 ccgacgattt cttctaaaag gtcttctagt gttgctaacc caacgacgcc gccatattca
    13081 tctaataaaa tagccatctg attctgcgtc tttttcattt catataacag atcatcaata
    13141 aagaccgttt caggaacgaa taaaggctcc tgcatgattt tcttgatatc taaattatca
    13201 aagccgaatt tatgggcagc ttttagtaaa ttctttgtat gaaggattcc gactacttta
    13261 tctttgtctt cgttataaac agggattctt gaatagtttt cggataaaac ttcattcaca
    13321 ttttcttcca cagtgtcgtt gatatcaatc ataaatgcat ccgttcgtgg gaccatgact
    13381 tctcgggcaa ctttcgtatc aagagaaaaa accccttgga gcatttctaa ttcttcatta
    13441 ttcaatacgc cttcgttttc aagcatataa cgcatttcat ctctggtcat ttttgaatct
    13501 tcatcatcaa aagtcatcgg cgtgatacgc gccaataaac ttgtggaagc agaaagcagc
    13561 cagacgaagg gcttagctac tacaccaatc aagcgtatca gccctgaagt gaactttgcg
    13621 acttcttctg atttatttaa agcaatacgt ttagggtaca attctccaaa aacaatcgat
    13681 acataggtca aaatagccag cacaatgata tttgcaatat tttttgccgc tgtgccgcca
    13741 cctaataccg gagctaaccg ttccgataaa gtattagcta gtgaagcacc agacaaaatg
    13801 ttgaccaaag tgattcctac ttggattgtc gataggaaat tatttggatt ctgcaatatt
    13861 tttaaaagct tttgagattt cacatctcct tcatcagctt tttgctcgat tcgattctta
    13921 ttgatcgaaa caacagcaat ttcggaagct gctaaaaaag cattgatcaa tgtcaaaacg
    13981 atcaataata aaatctgcgc taacagcgac tgactctcag ggtcagcatt cataatcgca
    14041 ttctctcctt ataaaattaa tttttagttt acgatagaaa aaaacgtggc actatagcca
    14101 cgttaaaatg gtatcacaaa gcaattttct ttgcaataaa aacaagtgta ctaaaactac
    14161 ctacgaggtc gtagcaattt tcttcttgtt ttcattttgc ttgtcttctt tgacgatttt
    14221 gattatatag gaaatgatcg tcttgcagat tgcgtaaaaa ggaacaccta agaagatacc
    14281 aagcaatccg gcaatatttc cagctactag aagaacgata atgatcgtta atggatggat
    14341 tttcagtgat ttcccaatta cattaggata aatgatattc ccatccaact gttggacgat
    14401 tagcacaacc acacaacaaa gtaatgcttt taccggatca ttgaagactg ttactagaaa
    14461 tgcaggtgct agtcctagat aaggtcctaa atatggaatt aggtttgtca atcccgcaat
    14521 gaccccaaat aagaaagcat aatccacacc aattaccata tatcctaaaa atgtgaaggt
    14581 accaacgaat aaacattcga ttgcttgtcc gctaatgtaa ttagccaatg ttttattcag
    14641 ttgccccaat aactccacaa tttggtttct tcttttttct gggaaaaact gttggatgtt
    14701 tggcactagt ttttctccgt ctttcaacat atagaacaag atgaatggtg ctgtaatgat
    14761 tactacggtt gccgaagcga tcgttccaac gatcgaacct aaactattgg atactccact
    14821 aaggaattgc tgaatgattg tcccgtaaga aatgtctaat tgctcaaaat atttattgat
    14881 atctacttcc ttgaacaaag gcaattcggc tacctctttt aaccagcttt ctacctgttt
    14941 gatgaaatca ggcatattag atgccaatga ggaaatttgc tgtactagac ttgggatgac
    15001 actaagtaaa atccaaataa ttgctgcaac taaaaggagc agcacaattg cgacagccca
    15061 tattcttttg atttttgctt tcatcaacag cttgacaata ggattcaata aatagtaaag
    15121 aaagcctgca atcagtacag gtgcaaataa cgtggaaaaa aatgtcccga tcggttcgaa
    15181 aataaagttt atttctgatg aaatcatgat caatgcagct atgatcaata attcaagcga
    15241 ccaaaacatt agtttagact gtctaagttt ttcaaacaat agatttcctc ctttctttct
    15301 tactagtata tattatattc gttaaaaatt cttttattct ttttttattt ttgaaaactt
    15361 gccatacttt ttatttgttt tattttccag tatactatac gaaaggaagg tggctagacc
    15421 atgaaagttg tacaaacaaa agatacaatg agcaatattt acttagatgc tgtacggatc
    15481 aggcatcaag tttttgtagt cgaacagggt gtgcctttgt ctcgagaaat agacaaagat
    15541 gaagcccatt gtatccactt cgtcctgtac tcagataaaa aagaaccgca aggaactgta
    15601 cgtcttttgc ctttagaaaa cggaaaaatg aaattacagc ggatggcgat tctttctgaa
    15661 tacagacacc aagggttagg aaaaatattg attgaagaag cagaaaactt tgctaagaat
    15721 caaggctata atacgatttt attaggggca cagtccacag ctgaaacttt ttacgaaaaa
    15781 ctaggatata cagcttacgg cgatcctttt gaagatgctg gcatgcctca tatttatatg
    15841 aaaaagactt tataaaaatt tatttcgaaa aaagcaaaaa acagcaagct tttgagaaaa
    15901 atctctcact ggcttactgt tcttttttgt gtttacttac gcgacaattg cttgtaacgg
    15961 ttataccaaa tatcaatata gccttccgag aacgggccat ctccattatt gatccagtca
    16021 accagaatct tcacattttc ttttaggatg aagtcaatat cgttcgggta attcatcatt
    16081 ttgctgtgca tctcatattc atcgacatcc aatagtcgtt tctcgccgtc aggaaatacc
    16141 ttgatatcca agtcatagtc gatatatttc aaggcctcat cgtccagcaa aaaaggtgaa
    16201 gctagattac aataatagga aacccccttt tctctgatca ttgctattac attgaaccag
    16261 tattttttat gaaaataaac aatcgctggt tcgcgcgtta cccatcggcg tccatcagat
    16321 tcagtgacta atgtgtgatc attcacgccg atcatagagt gttcgcttgt ttttaatacc
    16381 atggtatctc gccacgttcg atgcaatttg ccgtcgtgct tgtaactttg gatcgtcaca
    16441 aactctcctt cttttggaac tcccatagca acccttcctt aagcagcatt ttgtgctttg
    16501 tgtttgggtg ttcattacac cgacatcata attattatat caataaaaat gagctttgcc
    16561 tagaaaggat tcctattgtt tcgatttttc tctaactgat ctaccagttt catttggacc
    16621 ttaggaaaaa cgaccgaatc aaatgcatca ggcttcaacc acttcgaagt tttgttctcg
    16681 gtaaattctt gttctgctcc ctcggttgcc cgtccgtaaa aaaggagaac atgccatttc
    16741 agatgactaa aaatatgagt aacttctcct aaatgtcgtg tctgccaaac aaagagttcc
    16801 tgcttttcaa atgggagatt ttgctcttct tctgctacta ggtcatcaaa cagatcgatt
    16861 tctggctttg tttcccattc ttttttcaat cgctcatact cctcttgtgt cacttccatc
    16921 attggaaatg tccacatatt cgccagcaat ttttggctat ctctttcttc aaaataatac
    16981 gctccagaat gattttgaag agctgcactg atataataaa cgtcttttgg ctttgctttt
    17041 tttgttttta caggaaaact cgtttggatt cctcgtttgt tagctaaaca aaaggcttgg
    17101 atcggacaag tctcacactt tggagaagtt ggtgtacaga tagctgaacc tagatccatc
    17161 attgcttggt tgaattcacc tggatgcttt tcatcaatga ttttccgcat cgcttcatca
    17221 aagattttcc tgctagaagc ttttgcgatg tctgcttcaa tgcaaaacaa tctgctgact
    17281 actcgcatca cgttgccgtc aactgcaggt tctggaagtc caaatgcgat acttgcaatc
    17341 gcgcctgtcg tatatggtcc tattcccttc aatgagctaa tttcttcagg cgtttgcggc
    17401 atttccccat caaattctga catgatctgt ttagcagcag cttggatgtt ccgggctctt
    17461 gagtaataac caaggccctc ccatgctttt aaaagctttt cttctggagc atttgctagc
    17521 tcttcaattg taggaaacca ttccatgaaa cgataaaaat aatcgatcac tgtgtctact
    17581 ctggtttgct gaagcatgat ctcagagatc cagatacgat aaggatcacg attatagcgc
    17641 caaggcaggt ttcttttttc ttgttcatac cattgaatga actgatcttg aaattctttt
    17701 gtttctttat ctgtccattt ttcccattct ttattcactt tcctcttcct tttccgtaat
    17761 gaatcgctgg atcatatcca aatcaaatcc tttttgatat aagctttgct ttacttttag
    17821 atttcttttt ttaggttcaa accttaaatt tcttcgccat agtttatctc cttgtttgac
    17881 taaataatca tattctattt cttgatctgc ttcttgcgga agatcagcta tcgcttgttg
    17941 gatcacttct tgagaaaagc ctttcgtcat caatgtttgt tggatttttc tcaataattc
    18001 tttttggctt tttccgtgat tttttccaaa cgctttttcg gctgtctgat gtgcattttg
    18061 aacttggtct tcaaatgtat attggctgag cgcttgttca atgatttcag gtttcaaacc
    18121 tttttgctgc atctgctgcg ctaatttctt tggtcctttg tcacttaagc gcatattcgt
    18181 gcgcacataa ctttccccat agaccaaatc atcgacaagg tccaattctt ttaagcgggc
    18241 tacgatttta tttcgatctt cttgaaggat ttccttttct tttaagtatg ttcggatttc
    18301 tttttccgat cgcaactgat aacttaagta attcattgct aggtgaaaac cgaaatcagc
    18361 tccgctactc ttcttgatct cttggatagt ttcttcgtct aattcccgtc cttttaataa
    18421 tcgatagcgt actaatacat cttcagatac ttgtaaagct tctccttcag ataaatctac
    18481 gcgatagaaa gggcctttgc ctttactgat tctcgtaacg gttaacataa acatcctcct
    18541 cgaacatata ttctctatca taccacagaa aatccccgta atcaggtatt atatgttaga
    18601 ataaaccaca gaaaaaagaa aaagggagat acttatggca gaaacaattg tcaaaactgg
    18661 acaaacggtt cctttgaaaa tcaagcgttt aggcataaat ggtgaaggaa tcggttattt
    18721 caaacggttg atcatatttg ttccatatgc actaccaaaa gaagaagtac tagtgaaaat
    18781 cacgaaagca actcctcgct atgcagaagg agaattaata aaagtcaaaa aaacaagcaa
    18841 agatcgcgta gtggcaccat gtcctgttta ctatgaatgc ggtggctgtc aattacaaca
    18901 cttagcttat caagctcaat tggattttaa aaaggatctg cttttgcaag cattggaaaa
    18961 attcaaacca gcaggctttc gatcttatca acttttgccg acgatcggga tggatgagcc
    19021 ttggcattat agaaacaaag cacaatttca gttacgaaaa aataagcaga ctcaaaaaat
    19081 tgaagctggt ttatacgaag caaactctca tcagttagtc cctataacag actgcttggt
    19141 tcaagaacct cgaacacaaa aagtaatgaa tactgttgtt caactattga ataaatttga
    19201 tgcacccatc tatgatgaac ggacaaacag tggaatcttc cgcacaatca tggttagaat
    19261 cggtgttaaa actggggagt tacaagttgt ttttattact cgcagtaaaa aattcccgca
    19321 aaaaaacgca atgatccgtg agatcaatga acgactgccc gaagttgttt caatcatgca
    19381 aaatgtccag gcacaaaaaa cgtctctcgt gatgggggat gaaacgatcc acttatgggg
    19441 aaaagaagcc attgaagaac aaatcaatga agtgactttc gatctttctc ctcgtgcctt
    19501 ctttcaactc aatcctcagc agaccgaatt gctttatggc gaaggaatca aagcattaga
    19561 tgtccagcct aatgaaacga tcgtggatgc ttattgcgga gttgggacga tcggcttaag
    19621 tgtagcaaaa caagccaaag aagtacgggg aatggatacc gttcctcaag caatcgctga
    19681 tgcaaaaaaa aatgcggaaa gattaggata tcacaacact cgatatgaag tcgggacagc
    19741 agagcaagtt ctgcctaaat ggttgaatga aggttttcag cctgatggta tcatcgtaga
    19801 tccaccacgt acgggtttgg ataacgcatt gatccaatca ttattgaaat accctccaaa
    19861 aaaacttgtt tatatctcct gtaatgtttc tacacttgcc agagatctgg ttaatttaag
    19921 taaaaagttc aagattgaat atttacagtc cgtcgacatg tttcctcaga ctgcacgttg
    19981 tgaagttgtc gtgaagatga cgagactcgc tgactaaatg aaagaggttg tgaaaaagtc
    20041 tgccctgtat caagtaataa gaacagccaa acaaaaatag ctcttcatgt tttatgaata
    20101 ctgtacttgt gaccgaagag ttaacaggag gacactattt atttaaaaaa agggttgtga
    20161 catagttttt gtcacagccc tttttgatta ttattggata ttcacgtcta tcacttgata
    20221 aaatgcattt gtcgtatcgc tgatattcca tactgctaaa atcacattat atccttttcg
    20281 atcctgcgga agtgtgatcg tttggttgac tgtttttggt ggaacagttg ctttgtcgtc
    20341 tatttttgtg atcaattcaa aatcactaaa tttcaaaggt tgattaggat tccaagttgg
    20401 tttggtaatg tagtaatccc aagtacttgt ccgatgttgt gctgttaaat tccaagtaat
    20461 atttaatgtt cctggattaa ccatgttttt atgccatctg ttagatgtct gttcatctaa
    20521 aggctcaaag ccgctgactc ctgcactagc aatttttcca tcaatgaatg tatttttggt
    20581 agcttcgata ctttgcggtt cccattgtgc tcttccgaca ttttgattta agtttcctgc
    20641 tgctgatgtt cctaggtatg cacggcttcc tggagaagta atataaccat gcgcaaaacc
    20701 tggaatgcta aaaccgaaca accctaaact aactaaccca ataaacaata acgtactaat
    20761 ctttttcatg tagaaacctc cttcatttga taaccacatt gtaaacgctt tattgtatta
    20821 acacaaacat tatgctttta taattttatg ttgaagtaaa aaaggtgaaa aaagtaagat
    20881 gcagattata acagaagtag acgagaaaag tacaaataga aaacatgaga atcaaatcat
    20941 atgatctttt tcaaaaacat cattcatata aacaaatgtt caacttttta ataacaacaa
    21001 ttatcaattt ttttcttact tcacaaacta aatacaggtc ttgctgtgag ccttcatggt
    21061 aaactaatat tatagaaagg agttagtaaa atgaagatga taaaagatat tcgtttttgg
    21121 gtttgtgtta taggaattgt catattaggc tttttatcag ggttgcttag cggaaatccc
    21181 ggaaaatatt actattcttt gcaacttcca ccatttgcac cacctagctg gatatttggg
    21241 ccgatgtgga ccttactgta tatattgatg ggtatttctc tttatttgtt acttaatcac
    21301 aacaacaaaa aacagcgaaa caatcttgtc ggtctttttg tgattcagtt catttttaat
    21361 tttatatggt ctgctctatt ttttaattta agaaatattt tcattgcagc aatcgatatt
    21421 acacttttag ttattttctt gtcagtgctt atgtatcagt tgtggctgca tcatcgcctg
    21481 gcaatgtggc tcatgattcc ttattattta tgggtactat tcgcaactct attgaattat
    21541 tcaatttatt ttttaaacta agcgctaaaa agagacgtcc tgtaatttaa cacatagaaa
    21601 aagtgccacc taagttagat tttttgtcta atttaggtgg cactctttat taaccaaact
    21661 atttttttct agtacccact tgttgttcca tactcattat tctggtcata accataacca
    21721 ttttgttcct gtccagcgta aggatcataa ctttgagtat cttgacttgg tgtatagtac
    21781 tgacttgtat cttctgtttg ctgataatag gtattcggat cttggtactg atcatatgtg
    21841 ttttctgaat aggaagaata atcggtgtac ccttcactat tcgatccatc accgtttgtc
    21901 gaatcgccat cttccgaatc atcaaagaac atcattgaag cattctcatt tttcaaatta
    21961 ggtaatgtat cgcttgtttt tatatttaat tgtttcttca gttcattttg gatcgaaaga
    22021 agatcatttt ttcctaaaat ttgatagtag atatcattga tcatttgtcc ttcgccttgc
    22081 aactggtctt gtttgattgt ttcaaacgcc ggggtgtagt tcgtcgctac atcaagcatg
    22141 tcatcccaat ctaagtccgt cgtcacattg ttctcaactg cttttaaaat ttttctataa
    22201 ttgcttacgc catctaaact taaaacttta ttgacgattt tcgtcactac ttcccgctga
    22261 cgacgttgtc gaccgatatc gccttctgga tcttcgtgtc gcattcgaga ataggctaaa
    22321 cctttttctc cattcaatgt ttgttcacct actggtacat ggacaccatc caaagtaaag
    22381 tcaattttat tattcacttt gatgccaccg accgcattga tcaaatcttc catgccatcc
    22441 aaattgatcg ttacataatg atcaatagga atatcgagca gttgctcaat tgaatccatg
    22501 gccatttcga caccaccaaa tgcgtaggca tgatttaatt tatctaccgt tccataacct
    22561 acgatattcg tataaatatc tcggtccaaa ctgatgatcg ttgatttttt ttgtttcgga
    22621 ttaactgtta ctaccatcat cgtatcagaa cgtccttgtt ccgttcttcc taatccaccg
    22681 gtatctaaac ctaatagcaa aaccgaaaaa ggttctttgt catcaatgct tactctctca
    22741 gaacgtttgg aggatattcg attagagttt ttagaaattt tgtttactgt ttggctcgct
    22801 tctcccataa atctgattcc gtatgctgat aatccggcaa tcagcaaaac gaatacccct
    22861 aacaaggtta atagaacttt ttgccatcgc ttcataatac acctctgttt cacattttct
    22921 aaaatattgt ctcatataac tgttcaatca gcaaattcac tttttaatca taagaaaact
    22981 ttaaaaaaca tttttgtgta acgattatta ttttttaaaa tttctcaaaa ttcttaacta
    23041 tcataccata ccgtgaaaaa gatcgaaaga ccgaatacac gcagttcgca atcgttttat
    23101 ctttttttat gtattttttc cagaaaaagc gaacaacaag gtattttcca aataatcgtg
    23161 cagtaaaatg atcgcacata cctttcccat ctttttaaca gtctttgcct accgttcaga
    23221 ttatcattcg atttcacgac aatttcttcc tatgactggt ccttttgtta aacccatctt
    23281 ctattcgttt tatttccagc tcactgcatg gctcttaaaa tgactcgaac tagtgattgc
    23341 attaccatca tcttttaatg gatagatatg ctcctcttca aagtaaaacg attttctttt
    23401 aaagtaatag aattgacatg gtaatgagta tgtatactag tggtttcaca aatggtcttt
    23461 tattcaacat ccaaaaactg aaatttcttt aaaatccatt actaacgaag aaaatgttcc
    23521 ttgcttgata attagttagg acatgtcaag tgaacaaatg catttactta gccaaaaaat
    23581 ctaaaaactt cataaataga caaaaagaca cgactctcta agaagtcgtg tctttattca
    23641 acaaattatt ataatttagt cacttcagca gcttgaggtc cgcgtgcacc ttcaacaatc
    23701 gtgaattcta cttcttgacc ttcttctagg ctcttgaagc cgtctccttg aattgctgag
    23761 aaatgaacaa atacgtcatc tccgccgtct actgtaataa acccaaatcc tttttcattg
    23821 ttgaaccatt taaccgttcc gtgttccata aatgtatatc ctccaaaaaa gttcttgcaa
    23881 gtaaccttgc aagaacatta tacccaattt agaaaacgct agcaagcttt cgctctttta
    23941 ttttccaaat tatttttaag actgatatga cggaggaaat ggttattttg ctgcttcgaa
    24001 acgtttgttt acttcatccc aattaataac attccagaat gctgcaatat aatctggtcg
    24061 gacattttta tatttcaaat aataagcatg ttcccaaacg tccaaaccta gaattggtgt
    24121 ctttccttcc atcaatggag aatcttgatt tgcagtagag gtaatagcta attttccatt
    24181 ttccattaca agccaagccc atccagaacc aaatcgtcca gcggctgctt ttttgaattc
    24241 ttctttaaaa gaagaaaaat caccaaaagc ttcattaatc gcttctttta tttctcctgt
    24301 aggttcgcca ccagcatttg gtgccatgat ttcccagaaa aatgaatggt tagcatgtcc
    24361 gccaccattg ttacgtacag ctgtcttgat atctgttgga atagcgtcca tatcagataa
    24421 taattcttct attgtttttt cgcctaattc tgggtatttc tcaattgctg aatttaaatt
    24481 cgtcacataa gtattgtgat gcttatcatg atgcagatgc atcgtttctt cgtcaatata
    24541 aggttccaat gcgtcatatg cataaggtag atctggtaat gtgtaagtca taaaaaaatt
    24601 cctcctcaat gttttcttaa taatgtctac attacaaaga gtaacaaaga gaaggaaatt
    24661 cttcaattgt tatgcctagt cgtctttttc actttgacct acttcttctt caaaaacctc
    24721 agtttcttta aaaataaaca acttgctgaa aatatagttc gcaaggataa caataatttg
    24781 agtgatgatt ttcgccacat actcattgct gttcataaca ttgatcaaca ataccattgc
    24841 ccccatgtcc aatccgtaag aaaggatacg gtagaagaag aacttaccgc attcaataag
    24901 tgcatgtgta aaattcggtg attttgaatg gaatacccaa agtttgttcg ttacaaaagc
    24961 aaacaataca gaacaaaacc acgaaataga attggataag gccatactta aatcaaaata
    25021 ttggcgtgcg agagcaaaac taacaaaatt tacaatcgta gccaaaccac caaaaaagag
    25081 atagactagt atctcccaat atccttttgc ttctaaatat ccttttaact gtcgatactg
    25141 cttcatgagc caacctacct tattaaaaat tcttatttat catactctat atttcaataa
    25201 ctttcatctt tatttaaaaa aattaatttt ttttgatcct ttgcctttgt ctctgcttgg
    25261 atcgtcacat gtgtaatacc atattttaat tttaataaag aaattatacg aggatatatc
    25321 ttttcaatct cgcttagtag ctgatcttca aaatcaatat gtccgtcgaa aataattatc
    25381 ttttcgctat actgccaaat atggacatga tgaacatcct ttaccccatc gattgcttca
    25441 atttcttgtt ccattttttc atagtcgata gcaggtgcac tttgtatcaa aatagcggca
    25501 gcttttttga tcaccattat cgcttctatc aatatatata tcgaaatgat cagtgtaata
    25561 actggatcga tccagtagat atcgaaaaaa cggatcaaaa ttgctccgat aatcacactt
    25621 accgaagaca gtgtatcact taacaaatgc aaataactag attttatatt caaactttca
    25681 tctgcttctt gtcgtaataa taatactgag aacaaattcg cgagcaatcc aataactgct
    25741 actgttagca tcaaatgact atttatcttt tctggtgaag tgaaccgctt gaatgcttca
    25801 acggttaaaa acaaggataa tatgatcaat atgaatgagt tgatgaaagc actcaatatt
    25861 tctaaacgct ggtagccata cgtacgtttt ttatttgctt cttttcgggc cattttttgt
    25921 gccccgtaag aaaaaataat tgccaatgta tcactaaggt tatggaaagc atctgaaaca
    25981 agagacaaac tccctgacat aatccctcca ataaattctg ataaagtgat caccgcattc
    26041 aaaaccacta ctccaaatag tttttttgat gatctctgtg gctgtgtagg cactattcct
    26101 tcctccattt tatttatatt ggatagtctt tagacaagaa taacattttt ttgatatttc
    26161 accaatcatt cagctgtttc cttaaagcac ttgtatttca cttgatactt cgctagaatt
    26221 gcaagtgatg acactactag atgaggagta cttatgacaa caaaacaatt aattatcagt
    26281 tcctttatac gattcaaaga cctaaaggag gcgagaaatg tcccattttg gaaagccatt
    26341 gtctatcttt tagttttgag tataattatg gctttgccaa tctcttttca agtttttcaa
    26401 gtcttaaaca acattcaatt ggatgggcaa aagattgcta ctcgcattcc tgattttaca
    26461 atccaaaacg ggcagcttca aaccgaagaa aaagaaggat tcatttacca aacaaactcc
    26521 attatcttca catttgatcc agagggaaaa cgaacagaac aggacatctc tactgatttg
    26581 atggggaatt ttgttagtgt cgggatgtta aaaaacaaac ttatcatagc tcttcctaat
    26641 acaggcacga cttctgcttt gttgaacaat aaccaattag aacttcccta caccaatgaa
    26701 tctttaaaaa acttaacagg taagcagctc cgttcttttt taagtgaagc atctatacca
    26761 ctttggataa aagtcctgac atttatattt tctatttatc ctagttttct gaatctgctg
    26821 atcactttgc tatttaccaa cattgctgct tttctctatg cacgctttcg actaacaaaa
    26881 gctacttttc ttgattgttt aaaaacattg atctattcaa tttcattgcc tactataatt
    26941 gcaacaatct tgatggtctt tcttccttct tttgacacta gcgccttcat tgcttttgca
    27001 ggaatcttta tttttgctca agctgtcaaa ggatgggcaa aaatatcgat caattaagaa
    27061 ataaagacaa ctcgttattt ctacttcaat agaccctttt agaggttgtg acattttttt
    27121 gtcacagccc cttttttatt tgaaaaatat ttctttctca aaaaacaagc aaggcgcttc
    27181 aaagttcttc ttgtatgcgc tacacttaga gtaaagaaag gcgggaaatt aactatgtca
    27241 gaaagaatca tttatgcaga tcgtccattt cgtgatgaat ttatcgaaga aatccatact
    27301 atagcgcctg gttatatctt taaaacagaa ttacaaccag aggatctaca aaatgtagag
    27361 atcagtttgg gctggaactc agactaccaa gaaaaactat tagcctcttc taccttgaaa
    27421 tgggtccaat ctatctcagc tggtgttgat cagctacccc ttgagacttt ttcaaagaaa
    27481 catattttac tttcaaatgg tagtggcatc cacagtgaat cgattacgga gcatgtctta
    27541 ggaatcatcc ttgggtattc tcgtggtctt tttcaagcac aaaaggcaca gagtgacatg
    27601 aaatggttag gttcatcggt ccattaccaa tctatccaag ggaaaaaaat actaatcatt
    27661 ggaactggcc atattggaaa aatgattgca aaaaaatgca aagcattaga catgaactgc
    27721 tatggaatca atacatccgg tcatccagca gaaggaatgg aaaagacttt ttctttaaat
    27781 caactaaagg aagttcttcc tgaagtagat ttcgtagtca atatcttacc tctaacggag
    27841 gaaacaactg ggctctttga tcagcatatg tttgaacact ttgatccaaa atctgtattc
    27901 atcaatgttg gccgtggtgc ttctgttaag acacaagatc tggtgcaggc tttaaataac
    27961 cagcaacttt cctttgcagc attagacgtt tttgaggaag agcctcttcc acaagatcat
    28021 cctctttgga aaatggacaa tgtactgatt acttcccaca ttgcaggttt gacacctgat
    28081 tttcaaaaga aattaatggc tattttttta accaatctga aaagctactt tgccacccat
    28141 gagcttcaaa ccaatcaagt aaaattgtct gcaggatatt aatagctgaa aacaatattt
    28201 tcaaaagaaa aaagagaagg tgacaaaaat tgtgtcaccc tctctttttt tattgttttt
    28261 taaagtgata ggttggctcc tccaggttgg gattataatc aacaagtaaa tcatatcctt
    28321 taaagaacca ttcatctgct tcctcgataa aaaaaagtgt cccatcaatt tccactttct
    28381 ttacaggatg ttctggctgt tcaactgaca ttccaatcga aaaaccatca tgaacctctg
    28441 ttttgccgta gacttttcca aaaaaacgta ttccgtaagt ttctggcaga tcaatttctg
    28501 agtcaaacca cttttgtgct tttggcgtta ccgttaattt catcccaaca acctactttc
    28561 tcttttcttt ctattgattc atcttatcgt ctagaatatc cacatcgcct gtctctccaa
    28621 taaccagtag attatcattt tcacgaacat attcatctgc tgcaggagaa gcgataaccg
    28681 tttgatctac tcttcgtatg gcgactactg ttaagtcaaa acgttgcctg aaatttaatt
    28741 cagctaatgt tttgttgaaa aatttaggat tggagactcg gatttctgct aaagaatatt
    28801 tatctgacaa ctctaggtaa tctaaaatat tctttgacac taaattatgc gcaatcctta
    28861 ttcccatgtc tcgctcggga tggacgacgc gatcagctcc gatcttttca agtactctgg
    28921 catgatactc attttgggct tttgctaaaa tatttggtac gcccatttcc tttaccatca
    28981 gagtaactaa gatactggct tgaatatctt ctccaatagc gacaattaca tgatcgaaat
    29041 ttcttatgcc taatgatcga agtgtcattt catcctgtgc atttgccaca acagcatgag
    29101 tggctatatt catatattca ttgacccgat cttcattact atcaatagcc agtacttctt
    29161 gaccagcttc gatcagtgtt cggcagatac ttccgccaaa tcgtccaaga cctacaattg
    29221 caaaattttg tttcaccgtt ttcctccttt agtatcttct tttctcagta taacaaagcc
    29281 tttacaaaaa caaaagcaag catcattatt gtaaaaaagc caagaaaaat aatagttatt
    29341 ttctcagctt ttgattatcc tctttatcat cgtttttatt tcaccaaacc atgtttgaat
    29401 gcatagatag cggcttgtgt acggtcttcc acttctagct tagccaatat attggaaaca
    29461 tgtgttttta ctgttttcag tgtaataaac aattcgtccg ctatttcctg attacttttt
    29521 ccttcagaaa tcagcattaa aatttcattt tcccgattcg ttagttcttc atgtaataca
    29581 ggctcatttc gccgactcat tttttccatc atttttgttg tcacttctgg ttctaatact
    29641 cgctcgccac gttgcgttgc ccttatagca tcagcaattt catgtgcggt agaggtcttc
    29701 aacaaatacc ctgctgcacc tgcttcaatg gcaggataaa ctttttcgtc atcaataaaa
    29761 ctggttacga taatgatttt agcttctggc caatctttta agattgcttt tgttgattcg
    29821 ataccatcca tttcttccat gactaagtcc attaaaatga cgtccggtcg aagtgtcatc
    29881 gccttctcat agccttgtcg tccattttca gcttctccaa ttacttcaat gtcttcttga
    29941 atagataaat aaaaagagac gcctaagcgc accatttcat ggtcatctac taataaaact
    30001 tttatcatcc ttatctatgc ctccttcatc aaagggacct tgatttctac acttgtacct
    30061 tgacctttaa aactaatgat ttttactgtt ccaccaatac cagctactcg ttctttgata
    30121 ttgttcaatc cataacttcc cgttttcgtt tcattcatat taaaccctgt tccgtcgtca
    30181 ataatacgta acaaaagatt attgtctatt ttgtgcaaat atacctctaa ttcattggct
    30241 ttagcatgtc ttaatgtatt tgataacaat tcttgaacga ttcggaacaa gtgatcctca
    30301 atggaactag atagttttac atcttctaca tcccatttca gcgaaatctg gattttattt
    30361 tgcaattcct tcaataactg ctcgatacct tgctttaaac ttttctcttc taaattgact
    30421 ggacgtagat gaagaagcag cgcacgcatc tcagactggg aagcgttgat gatctctgct
    30481 accattttca actgcttgcg aaacatttct ggtgtctcgc ttttttctgc ttgctccgtc
    30541 aatgccgaca tcatcatcat tgctgcaaac aattgttgcg aaactgaatc atgcagctca
    30601 cgggctaatc gatgtctttc taattctaaa atttcttctt tggtttgtcc atctacatat
    30661 tggggacggc tcgttacaat ttgtaattca ctagatactt caatcatttt ttcttttatc
    30721 ttagtgattt ccttatcaat atctttgatg tataaatcgt ccgatgcact tgggatcgga
    30781 agaagcaaca atttggattc ataattgcca gaagaaagtg cacgcagctt ttcttcgatt
    30841 ttcccgtatt ttgtcttttg aataagtgat aataatagaa agacaattaa tcctacacct
    30901 aaggaaatgg ctaacaaatg gaagataagc ggaacatata ataaacgtgc acgcagtaat
    30961 tctccccacc aatgactttg attgctggca taaaaataag tgaaaagtga aaatagaata
    31021 ataaggaaag ccgcaatccc cgaataaaca gctagcatcg ctctggatat ttttcccatc
    31081 atattcgtat cacctcgaca tctccaatca aggtattagt aataatcttc aatctacgag
    31141 ggttttcatc ataatcactg ctgtagattt tgatttgttc atttttcaaa gaaaattgct
    31201 cttcttcaaa caagacattt cctactaatg tcgcatgctc taatcggatg gctacgccca
    31261 aaggtactaa gatccgtgtt ctgcctatcc cttttcttac aatcacaata ttatcatctt
    31321 ttggcaaaag cgtattgccc aaatctatga tagtatcacc agaaatgacg gcgatgttga
    31381 tatcgtccca ttcatagacg tcgtttccaa tacgttggtt accgaataat tgctgtcttt
    31441 tccgctcatt attatgtgtt tttaattgct ctgtttggat catcatgatc tgtttctttc
    31501 gccaaaaagc atttttggtt aaatctatcc ctgaaatctc tacacctttc agaccaataa
    31561 ataaaacggc aaatacagcc atcatccata aagcaggact gttgactaag ctaaaaaaaa
    31621 tgaccaaact tcctaatatc aattgaaaat tttgaaattt agtacgagga tgtttgcgca
    31681 tagctagata tatattgaaa ataccgaata tcactaaaag caaaagtcca gtattgttta
    31741 cgatttgcca aacagcaaat aacagcagta gtgcttcaac taccacaaaa aaacgccaag
    31801 aactattcat cgctctttct ccctcctgac acattctcat ttcatcatac ctattttttt
    31861 aactgttggc tataggactt tagatgtaag atcgtttgtt gaaatctaaa atacacaaag
    31921 agaaaaagat ctagagtgta tcatcagtaa gatgaactct ctagatcttt cagtcagact
    31981 acgcttgttc tactttgacg attttaacta acatatcgcc acctggagta gcaatagtta
    32041 cttcatcatt tttacgtttg ccaatcaatg cttgcgcaat aggagaatca ttggaaattt
    32101 tgccagaaaa cggatctgct tcagcgcttc cgacaatcgt gtactcttct tcttctccat
    32161 ctggtagttc gatgaatgta actgttttac caattgatac ctcatctgca tctacaccgt
    32221 cgttatcgat gatttgagca aaacgaatca tattttcaat tgttgtgatt cttccttcaa
    32281 caaatgcttg ttcatctttc gctgattcat attcagagtt ttcggaaaga tctccgaaac
    32341 cgcgggcaat tttgattcgt tcaatgattt ctccgcgttt gactgttttt agttcttcta
    32401 attctttttc taatttttct ttaccttcta gagtcattgg ataaactttc tctaccatgt
    32461 gtaacgtctc cttttttcga taaaataccg caaaaggtaa tcttttgagg aatatagtcc
    32521 aaaaaagatt accacgcttt tcttcaaatg taaatgtttt tctaaaaatg atcaatgtct
    32581 agagcctgtt tttctaagaa ttatttacat atttttccac taattccata tgttcttcat
    32641 aagttttagc gaaataaaca tttccagtgc tcgtatctgc tacgaaataa taatagtcat
    32701 tttctgctgg ttctaatact gccttgattg cctcttcgct tggattgtca aaaggtccag
    32761 gtccatagcc ggtattggta tacaaattat atggtgaatc tacagctgta tcttcatacg
    32821 tcactaattc tttgtgttca cctaatgcgt ataaaacaga gatatctgac tgaagtggca
    32881 tatttgcttt gattcggtta aagaagactt gtgcaatgtt tttacgatca ttttctttta
    32941 cgccttcttt ttcaactaga gaagctagag ttaaaacttc ttgtacagtc aaatttttct
    33001 gcttgatagc tgaataatag ttttgcatga ccgtattcgt cttgttcacc atttcaatca
    33061 ctagatcctt aagcgaagta ctcgtgtaat aatcatatgt ggctgggaat aagtaacctt
    33121 ccaaacggta ttttgtgttc tcagctttag atgcactttc caacagttct gggaattttt
    33181 ggtgcaactc gttaaagaaa gtctcatcat tcactaaatc taaaaattct ttcttgtctt
    33241 taccagttac cttcgctacc cgttcagcaa tctgatcgat atcgtaccct tctggaatgg
    33301 caattttccc atctgcaact tttgttggct cagaagtacc gccttcttgc aactgtttgc
    33361 caatctcgtc aagagtcata ttaggcgcta actgataata gccagcttga aagccagtta
    33421 aatttttaaa ttttgtataa taattgaaaa caatcccgct tttaataata ttgtccttct
    33481 ccaaaatctc accaatctgc ttgttagaag agccactcgg gatctcgact tggactaatt
    33541 ggtcatctga tttatctaaa ggcttcaacc cgctatctac ataacgatac acggtaaatc
    33601 caagaaatcc gccaataatc aataatgtaa gagcaatcac taaaatgatc ttcctcacaa
    33661 tcttatcttc ttttttacgc agatttctcc gcttgtcgat tatatctgct gtagctgcct
    33721 tactggcttc ttttttatct ttacttgggt gttgtttgtt tcctgacaaa acaactcctc
    33781 cttactatca agttcgtaac tttattcatt atacatgata tttgaattgg cgtgaatgat
    33841 tttatttaaa tattctatca aaaatgtaag tagtaaat
//
LOCUS       DADVXU010000017        33678 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.018, whole genome shotgun sequence.
ACCESSION   DADVXU010000017 DADVXU010000000
VERSION     DADVXU010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 33678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.018"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            214..498
                     /locus_tag="KB953_000826"
     CDS             214..498
                     /locus_tag="KB953_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HBB6865822.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(815..1198)
                     /locus_tag="KB953_000827"
     CDS             complement(815..1198)
                     /locus_tag="KB953_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HBB6865823.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIK"
     gene            complement(1200..1784)
                     /locus_tag="KB953_000828"
     CDS             complement(1200..1784)
                     /locus_tag="KB953_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HBB6865824.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1831..3306)
                     /locus_tag="KB953_000829"
     CDS             complement(1831..3306)
                     /locus_tag="KB953_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HBB6865825.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3487..3798
                     /locus_tag="KB953_000830"
     CDS             3487..3798
                     /locus_tag="KB953_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865826.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3913..6249)
                     /locus_tag="KB953_000831"
     CDS             complement(3913..6249)
                     /locus_tag="KB953_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBB6865827.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6391..7059
                     /locus_tag="KB953_000832"
     CDS             6391..7059
                     /locus_tag="KB953_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBB6865828.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            7062..8105
                     /locus_tag="KB953_000833"
     CDS             7062..8105
                     /locus_tag="KB953_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBB6865829.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8342..9223)
                     /locus_tag="KB953_000834"
     CDS             complement(8342..9223)
                     /locus_tag="KB953_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBB6865830.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9382..10110
                     /locus_tag="KB953_000835"
     CDS             9382..10110
                     /locus_tag="KB953_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBB6865831.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10266..10583
                     /locus_tag="KB953_000836"
     CDS             10266..10583
                     /locus_tag="KB953_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBB6865832.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10576..11184
                     /locus_tag="KB953_000837"
     CDS             10576..11184
                     /locus_tag="KB953_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HBB6865833.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11239..12030
                     /locus_tag="KB953_000838"
     CDS             11239..12030
                     /locus_tag="KB953_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBB6865834.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            12168..12656
                     /locus_tag="KB953_000839"
     CDS             12168..12656
                     /locus_tag="KB953_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HBB6865835.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            12707..13579
                     /locus_tag="KB953_000840"
     CDS             12707..13579
                     /locus_tag="KB953_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBB6865836.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13755..14117
                     /locus_tag="KB953_000841"
     CDS             13755..14117
                     /locus_tag="KB953_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBB6865837.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            14126..14878
                     /locus_tag="KB953_000842"
     CDS             14126..14878
                     /locus_tag="KB953_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6865838.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14892..16694
                     /locus_tag="KB953_000843"
     CDS             14892..16694
                     /locus_tag="KB953_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6865839.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16971..18011
                     /locus_tag="KB953_000844"
     CDS             16971..18011
                     /locus_tag="KB953_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBB6865840.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(18063..20207)
                     /locus_tag="KB953_000845"
     CDS             complement(18063..20207)
                     /locus_tag="KB953_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBB6865841.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLRLDKSFWLDLPLKK"
     gene            complement(20564..21937)
                     /locus_tag="KB953_000846"
     CDS             complement(20564..21937)
                     /locus_tag="KB953_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBB6865842.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(21937..22332)
                     /gene="rbsD"
                     /locus_tag="KB953_000847"
     CDS             complement(21937..22332)
                     /gene="rbsD"
                     /locus_tag="KB953_000847"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HBB6865843.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(22360..23277)
                     /gene="rbsK"
                     /locus_tag="KB953_000848"
     CDS             complement(22360..23277)
                     /gene="rbsK"
                     /locus_tag="KB953_000848"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBB6865844.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(23385..24398)
                     /locus_tag="KB953_000849"
     CDS             complement(23385..24398)
                     /locus_tag="KB953_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBB6865845.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(24479..25078)
                     /locus_tag="KB953_000850"
     CDS             complement(24479..25078)
                     /locus_tag="KB953_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBB6865846.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            25264..25965
                     /locus_tag="KB953_000851"
     CDS             25264..25965
                     /locus_tag="KB953_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6865847.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            25975..28686
                     /locus_tag="KB953_000852"
     CDS             25975..28686
                     /locus_tag="KB953_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6865848.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            29137..30765
                     /gene="treC"
                     /locus_tag="KB953_000853"
     CDS             29137..30765
                     /gene="treC"
                     /locus_tag="KB953_000853"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBB6865849.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(30825..32006)
                     /locus_tag="KB953_000854"
     CDS             complement(30825..32006)
                     /locus_tag="KB953_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBB6865850.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            32258..>33678
                     /locus_tag="KB953_000855"
     CDS             32258..>33678
                     /locus_tag="KB953_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HBB6865851.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAG"
BASE COUNT        10662 a         6038 c         6851 g        10127 t
ORIGIN      
        1 aggaagaggt tgtgagcctg ccgtttaact ctgagtcaca aggagcaatt tcaaaaaaca
       61 gcttctcatg tttgttgact attgtgactt gtgaccgagg agttggcagg agaacaccgt
      121 ttattcgaaa aaaggaactg cgacaagact tttgtcacag ttccttcttt tttttgctat
      181 gctatactag atgtaaaaaa taggagaaat aaaatgaggg aaatttattc ttttgaatca
      241 aaaatctatg cttccgaagt cggcaagggc ggggcatata ttatttttcc gtatgatata
      301 agaaaagaat tcggtgctgg aagagttaaa gtccaagcaa cttttgacaa tgtgccgtat
      361 gaaggcagta tcgtgaatat gggcgtgaaa aatccagatg gttcagtctg ctacatcctt
      421 ggactacgaa aagatattcg taaaaacatc ggaaaagaaa tcggtgatat tgtagcagtt
      481 acagtgaagc aaaaataaaa gaggttgtga caaaagacgt gtcacaacct cttgataaac
      541 ggtgttcaaa agccagtttc tcggtagtaa gtcaaattta tgcaaaaatg tggaaaccca
      601 tgtttacaaa atttttctta ctactcgaaa cgaagcggct ttatcacaac ctcttgataa
      661 acggcgttca atcagctgtc cctcggtatt agctcaaaaa cggcaaaata tgagaagcta
      721 tttttccatt tttttctctc atactcaggt cataacgctg atttcacaac ctcttctttc
      781 ataatggttt ttcaccatct ttataataaa ctggttattt gatatggaaa tctatttttt
      841 ccaaccaatc taaaatcttg taacctaact cttctgacat cggaaaagag gcgttgattt
      901 gtgcattgga gatatggtca acatggatac cggaagtgat cgtacaactt cccggaacgg
      961 cagttttgat gatcacagct attttttcgg ctaagacatc gtctttatgg aaccgaccat
     1021 cgtggctagg gaaacgtaca gtttgcatct ctgtatcacg agaaaatgta gtgactgttc
     1081 cgatatgtgg tgtatctccg cctgttaaag tgatcaataa gtctttaccg attactttaa
     1141 cagcagcttg gatcgtgaaa cctagtttgg ttacttcaac tatcgtttct ttttccatat
     1201 taatcaccct gccaacgttt tgaatgctca tttacaattt caaattgatc caaaagtttc
     1261 attgtatggt gattaacaag atcttggatc gtttgaggct gattataaaa agcaggaatt
     1321 ggtggtatga tttgcacacc tagtcgtgct aatttagtca agttttccaa gtgaatggca
     1381 tttaatggcg tttcacgagg aacaatgact aactttcgtt gctccttcat tgttacatca
     1441 gcagcacgtg caatcaggtt gtcgcctaaa ccaacagaga tacctgcgac tgttttcata
     1501 cttgccggta cgatgatcat tccatcggtc aaaaatgatc cgctggcgac agcagcacct
     1561 aaatctttgt ttgaataatg gtaatcaaac aggctttcga attcagttag tgataagtcg
     1621 gattccagct ttaagttctg ttttgcccat ggactgatga ttccgtgtgt ttctatcatc
     1681 ggatattctt ttagtttctt cactaggttg atggcataaa tggtgcctga agcgccactt
     1741 acaccaatca cgattttttt cttccgttgt gccaattgtt ccattttccg cactcctttt
     1801 tatgatgtca aaacagttgc agatcattca ttatttcaaa tatttttccc aatcttctac
     1861 ttcttggaat tttgcacgta caaaatgttc tttcatatcg aaaggaactg taccgtcaaa
     1921 gatagttttt gcacttatcc cacggaaacg aatagagcca ggattatatt ctggtaattc
     1981 tgatgggtcc aatggatggt tcctcattcc tgggatcacg atgatatctt tatctccttg
     2041 gaaacgagtg ttgaaagtcc aaactacatc gttcatgtcg aaaatatcaa catcttcatc
     2101 gactaaaatg actgtcttca attctttgaa ggctgataaa gcaagaatag ctgcttgacg
     2161 ctgaatacct tcatccgctt cgttttcttt acggatttgc atgatggtca tcaatttacc
     2221 accacctgca ggaggattgt agacatttac tacttttcct ggaatagctt tatcgaccaa
     2281 ggcaagaata cttgcttctg taggaatacc ggccatagat acgtgttctt cactgggacc
     2341 aatcgttgtt tgcatgattg gattgtcttt tcggtgagta actgctttga ctttcactac
     2401 ttgtacagct ggattagcat ccccagtata gccaggaaat tctggcattg cttttcctga
     2461 gttcgtgttg atatcttctt gcattgtttc atttggcatg atttctgctt caatgatgaa
     2521 ttcagcacga gcaataccca cttcatcgac tgctatacca tcaacgattt gaataggttc
     2581 gttacgcaaa gctcctgcac accatagttc attgtagcca agtggagtgg taggtggttc
     2641 gaaagtcgtg ccaatggcaa ttgctggatc taaaccaata ttgatagtga taggcattgg
     2701 tttattcaag gcttcgaatt gtttttggaa gtgaccaatg tggcgtccgc caggcatgat
     2761 gtacataccg atcgtatctt tatcttctag aaccatacga tggatcgtta catcgctcat
     2821 tgttttttct ggatctgatc ctaagactac acccattgtg atgtatggac cagcatcttc
     2881 aggagtattt gtaggtgcag gcaaaatgtt gcgaatatcg aagtctgcat cggtagctag
     2941 atgaacgact tcttgtgctg gagcgtctgc tttgtctact ttcacaggtt tgattggatt
     3001 ttctacagcg tctttcaaga aacgaccaag tgttcgataa tcatggtgca aaatttcacc
     3061 tacacgttta cgactagcca ttgttccgat gttcacacgt gtgccgggga aacctttgat
     3121 attgttgaaa gtcatagctg gtccttcttg agtcggacgt tttactgtac cgccagcacc
     3181 gatataacgg taaacaccag ataattctgc ttcaggatcg atttcaatat ctgtttcgtg
     3241 atattgacct ggaagtgttt ttagttcttc gatgactttt cttaaatcat acggttgatc
     3301 tgtcatgaga gattcctcct tagttgtctt tacctatcta tagtaaaagg tgaaaaagag
     3361 gaaaacaatt caacttgttc ggtaaagtat tctacaatgg aatggatgcg tttttttgat
     3421 aatagaataa actatgatat acttatcact aaaagaaagc gcatacttag tgttggagga
     3481 aggaaagtga aaaaaatgga ggagtcattt cctaaagcag taaaagtgga aaatatagcg
     3541 aatattttga aagttacatt tgaaaacgga gaagtaaaat acgtgaaatc tcattggaca
     3601 gaagagataa ccgatgcctt gcaatttgga aaaaaaggaa gaggaaaaag aaaaaatctt
     3661 ctcgcacttt ctcgtaatat gtggattggg actgaagtaa cgattgaagc agatggaaca
     3721 gtattcataa atggaaaaga caggtatacg ccagaagagt tgtggtacaa aggaaagaaa
     3781 agtataccag aattataaaa aataattaaa aaaaacagcg cgaaacaagg aacatattac
     3841 ttcctcgttt cttgctgttt tctactattt cagcgctctt atgactcaaa tctaaatgtg
     3901 gttgtcacaa ccttattctg cacgcaaagc gatagcggcg tcttttttcg ctgccattcg
     3961 tgcaggaaga tgaccgccaa gcatcgtcaa tatagtagag acgatcacta gaataatcgc
     4021 atggacagga tttagttgag caacattttt tagatccgtc atgttgtata agatgctatt
     4081 gatagggaac gtagctagcc atgcaataaa cacacctaat atcccagaag aaatacctaa
     4141 aatacacgtt tctgcgtcaa atacgcgagt gatgtctttt ttacgtgcac ctaaagcttt
     4201 tagtacaccg atttcttttg tccgttcaat cacagatgta taagtaatga tactgatcat
     4261 gatcatactt gttactaaag agatccctgc aaaagcaata aggacataag tgatagcatc
     4321 catcaatccg ccagtcagtt cagtcatcgt tccagctaaa tcagtataaa tgattttatc
     4381 ttcatctgat ttgcctttat tgtaatcatc tagataatcc aagatctttt ctttgtcttc
     4441 aaaattgttc ggataaatca tgatacttga aggcaagctg tctccaccta aataactaag
     4501 caatgtttgt ttggcatttt cgtcaacttt ttcagtagtt aagacattta caccactgtc
     4561 tctttgagct ttgacgattt ctgattcttt gttttcgttg acgatttgag tcgttaattg
     4621 atcgctataa gcgattcctg gagataagag attcattgta gaagaagatt tcacgcgtag
     4681 gatccccgag atcttcaatt catcagaagc attctgatac atcgcatcat aatctgtatt
     4741 agggataaaa ttcccagttg gaagttttgt atagtaagta ttgttgttta ctaatttgaa
     4801 tgttgtacca acgatatcat caaaatcaag tttttcgtct tctttcacat caaatccaag
     4861 attcttcaat gcgttgatat tagtattatt attgccgtct acgatcagca cgacatctgt
     4921 tgctgaggct ggataacttc cggcaagtaa agaataattg tcttttaaaa agttttcttt
     4981 gctagtgtca agctgtgtag ggaaacttga cactcccaca cctgtcattg ctgacatcgt
     5041 actagataaa gaaagagatt ccgtatcagg attttgattc gagaagctga caggctgtac
     5101 tttgccattt acatcacgta aaagattgat tcctgtagta cgggtaaatc cgatattatt
     5161 acttaagtta ggatcgatgt ctgttacgta atcgacgtat gtttgatcaa tcttattcgt
     5221 gtgctgtgct ttatcttctt cgctgacttt tgcagtaatc gttttgctgt ctggataatc
     5281 cgctttgctt gcacccaatc cggcatcgtc acgagtctgg ttagtcgtta ccttagaaat
     5341 agtaatcgga aatttcgcca tcgtttcagc ttgcgtgtta tcgatttgtt tttggaatcc
     5401 agaagaaagg gacagaacga tcgcgattcc gatgatccca atactggaag caaaggaggt
     5461 caagaaagta cgtccttttt ttgttcggat attgttgaag gaaagcttta acgctgtcca
     5521 aaaactcatc tttgttctgc gtaagttaaa ttgatcatct tttggtcgtt cgatgtgagg
     5581 atgggaatct gttaagatct taccatcaga aaattcaatg attcgatctg cgtattgatg
     5641 ggccagttca ggattatgtg tgaccatgat cactaatttt tctttcgata actcttggat
     5701 caattccatg atctggatac ttgtttctgt atctaacgct ccggttggtt catcgcacaa
     5761 aaggatatct gggtcattgg ctagtgctcg agcgatggcc actcgctgca tttgtccacc
     5821 agataactga tttggctttt tatgcatatg atttgtcaag ccgactcggt gcaatgcatc
     5881 ttctgcccgt tttcgttttt cgtctttaga aacaccactt aatgtcatcc caagttcaac
     5941 gttttcaata atgcctagat gaccaatcaa gttgtaactt tgaaaaatga aaccgataga
     6001 gttgtttcgg taggcatccc agtcactgtc cttaaagtct ttagtagact ttccgttgat
     6061 taccatatca ccggaatcat aattatctag tccaccaatc acattcagca tagtcgtttt
     6121 accagaacca ctaggaccta aaatcgctac gaattctttt tgtctgaaag cgacagacac
     6181 accatccaat gctttggtgg tggtttcgcc gactttataa tattttttta tatcttttaa
     6241 ttgaagcata atcacccttc tcccgtattg aaatattcgg tatactaata agtatatcag
     6301 aacaatgatc gtagtatacg aaataaatgt taactgaaga tgaactacaa gaaaaaatct
     6361 agaaattttt caaaaaacag agggagatca atgaatcgta ttttagtagt agaagatgat
     6421 ccgcaattac agttgcttta ccgttctgtg ttggaacgag cgggcttttc agtcatcacc
     6481 gcattgaatg gagaggatgc gctgaaaaaa ctagaagcga cacgtataga attaatcatt
     6541 acagatatca tgatgccgaa aatggatggc tatgacttgc tagactcatt acgtagcacc
     6601 agatttgaca cacctgttct aatgattacg gcaaaggccg attttgagga taagaaacgc
     6661 gggttcaaat taggagcaga tgattatatg acaaaaccca tcgatgtcaa tgagatgatc
     6721 ctacgtgtgg aagcattgct tcgacgttca aagatcaatt acagcagtag cttgataatc
     6781 ggacaaacaa aattagagct agatgcttat acagtgttac aagatcagca ttcgttgatt
     6841 ctgcctcaaa aagaatttct gcttctgtat aaattactgt cttatcccaa caagatcttc
     6901 actcgccaac agttgatgga tgatatttgg ggattagata cgaatacaga agaacgaacg
     6961 atcgatgtac atatcaaacg tttaaggacc cgttttgaaa agacggaaga ttttcaaatc
     7021 atcacagtac ggggactagg ctataaggcg gttatttaaa gatgaaaaaa aagctatttt
     7081 cacaattatg gatctatttt gctagtattg tcttcatttc gatcttagtg acactgctgt
     7141 gttttctcgg ttttattttc ttattgtctc gacattctat ctcgcctgct gaatcggctg
     7201 gaaaaccaac gttatacccg ttattcgtct ttgcggggtt cagtatggtc gtgggtacag
     7261 gaatatcgat ttttgttggg cggcgtatcc tgcatccaat cagtgcgcta gggacaaata
     7321 tgagtctggt agcaacggga gatttttcga ttcgaatgga tgaacagcag aaagtagcag
     7381 aagtacagca actgtataaa gatttcaacg ttatggtcca agaactcaat agtatcgaga
     7441 cactgaggaa tgactttgtg tccagtgtat cccatgaatt caaaacacca ctagcaacaa
     7501 tccaaggata tgttcaattg ttacaagccc caaatctctc tgacgaagaa cgccaaatat
     7561 ttttatatcg gattatcgaa agtatcacgc agctttcaca attaacagag aacatcttaa
     7621 aattgaacaa actggaaaac cagcgtatcc aactggaaaa gaaagagtat cgattagatg
     7681 aacaaatcag agaagtaatc gttttttttc agcctaaatg ggagaaagaa cagctagaat
     7741 tggatatcga acttgctgca gtaaattaca caggcaatga agagtttttg taccaagtgt
     7801 ggctgaatat catggataac gccataaagt acaatcaaat caatggacag attcatatca
     7861 aactatttga gacagccaca gagattgtac tggaagtgac cgattcaggt gtaggaatga
     7921 atgaagagac cagagatcga atgtttgaaa aattttatca aggagataca agtcgtcaaa
     7981 tttctgggaa tgggctagga ctttcactgg ttaaaaaaat tctagaactg catgatggac
     8041 gtattgatta tagcagtatc gaaggagtag gaacgacagc aatgatacgg ttaagtaaac
     8101 aataggaaaa agaataaaac ccctaaaaat tctattgaag gtttttaggg gtttttagtc
     8161 gtttttttgt gattagggaa atgttcttac ggttcttttg gcataaaaat cgctgctttc
     8221 ttattgaact acgagaaatg ttagaggtgt cattgaaaca aggacatgat gcaagaaaga
     8281 gagcgaaagt catgcgtcgc tctctttttt tgggtggata gatggacaga aaatgtaggg
     8341 cttagttttc ttcttgattt aaatactccc aaaatgtatc tgaagccaaa gaatgctcac
     8401 cgactttacg agtaaatgca agataactct ctgttgttgg agaaataggg agaatcacag
     8461 tattttcttt taatagattc ttgactgttt tttccataac aatagcaatc cccatgtcat
     8521 ttgcaatcat attgatgatc aagtcgattc ttgctccttt ataagagatt tttggttcaa
     8581 atccagcttc gcgacacaga tctagtacag gttgatacaa gttcgtagtg gggccaagga
     8641 gcaaaaagtt ctcctttttc agttcgctta gatccaattt ctttttacta gctaaaggat
     8701 gatttttagg tagaactgct acaaaatcgt ctgtttcagt aggcaaccac tcaatcgaac
     8761 ttggtggggc ctggaaactt ctagcaaagt ggatcgtgtc actatgttca ttggcgtcat
     8821 tgtaagttaa ttcgacactt tccacttctt tcaactggac tgtaaattca ggatggcgtt
     8881 tcaaaaactc ggtgattttg gaaaaacctt tataattgat cattgtcggt atcgttaaga
     8941 tatttaatga aagattgttg tctttcgtat aggtttcaag cacttcttgc atagcatggt
     9001 attgttctac gacatttttt acatatggga gcaataagct tccagcttcg gtcaattcga
     9061 tttttctatg ggcgcgttcg aataatttga cgtctaattc tgtttctaac gaaagaatct
     9121 gtttagaaat atttccttgg gtggtgaatt gtcgatcagc agttttggtg aaattcattg
     9181 tttcggctaa atcaagaaaa acttcgaatt tatggatatc catcatattc ctcctactta
     9241 ttccaataaa gaatacttta acacaaaaaa tgaattggtg taatcttctc tgtctgttac
     9301 aatctttctt gcagcgaaag caataattac agaaattttt ctgatttttt tcgcttcgta
     9361 gaaaaagaga ggagtttttt gatgccaact ggagttttga tcaatgtagg ttccgttttg
     9421 tttggcggac taattggcgg attagtagga aataaattaa gtgaacattt taaagcacag
     9481 ctgacgatgg tttttggtgt gtgttcgatg gggatgggaa tctattccat tgcgccgatg
     9541 aaaaatatgc cggctgttat ttttgcttta gtgataggaa cagcgattgg tttgattgtt
     9601 catttaggta atgggataaa taagggggct gcgctgatgc aagtacctat ttctaaaatc
     9661 ttccctagtg aaaagcttgg tatgacccac gatgaattta tcagcacatt agtgacggtc
     9721 attgttctat tttgtgcaag tggtacgggg atctatggaa gtttagattc tgggatgaca
     9781 ggagacagta cgattttgat ttctaaatct gtccttgatt ttttcacagc agctattttt
     9841 gcgtgtaatt tagggtatgt cgtttctgtg atagcaattc cccaattcat tattttttat
     9901 attttgttct tattggcaaa atttatttat ccattgacta caccagacat gatcttagat
     9961 tttaaagctt gcggcggatt cttgatggtc gctaccggtt ttagaatgat caacgtgaaa
    10021 atgttcccag tagctgacat gatacctgca atgatcgtta tcatgccact gagctggatg
    10081 tggacaaatt ggattatgcc actgttataa taaaaatcaa agagaagcca ttttttgctg
    10141 actaaaaagg atcaaatctt ttagtcagtt tttttgtatt tgacaaactg attttgaaac
    10201 tatattatat gttgtataat atagattgaa ataaaatatt acacttgaga atagaaggtg
    10261 acgtaatgca catccagatt ccaactgttc ttcttgatgg tactgtgcta gcagcattaa
    10321 gcaaggaaga tatgtatggg tatgccttaa caaaaatcgt acaagaaagt ctctctgtca
    10381 gtgaatctac actatatccg gttttgagaa gattgaaaaa agaaggatat cttgagacct
    10441 atgatgaacc ttttgaaggc agaaaccgtc gatattactc gttgacacca atgggaagtc
    10501 gtcatttaga agaaattatt gatgaatgga ttgtttttcg agattcttta gaccggttat
    10561 tggaggagag cacaaatgaa tgaatatttg aatgaagttg tggatcatct tcaagaaatt
    10621 cctgaagacg atcgttttga tttgatccag tattatgagg aatatttttt agattctgga
    10681 aaaacattag atcagatcat tgatgaatat ggtacaccaa agcagtttgc cttaaaatta
    10741 aaaatcagtt acttttcaga agaagaacaa gcagaaaacg agttagtgaa aaatccaaaa
    10801 agacaaattc gcctgatttg gttaatcatt atcggtttat ttgcttctcc tgtcctcatt
    10861 ccactagcac tggggtttat attggttatt gggggactat tggttgcttt actagcgctc
    10921 atttttagta tctatgtttt atgtatcagt atcttaggtg ttggcctatt catgatcatc
    10981 tcaggaatca gtgtgtttgg acaatcgatt ctgagtggat tattctttat cggtgtcggt
    11041 ctgttcgcca caggaagcgc agtatttatt tcgccactgt tgctgaaagg aacaaaaggg
    11101 cttttttatg tcatgatgaa ctttgttaaa tggatgggac gacgttttat tacaaaaaga
    11161 gatctgcatt cagcgggcgt ttgaaggagg acacataaat tgagattgaa acattttttg
    11221 attaccggta ttggtctgat gcttactggt ggcctcctag caggtgcaac ttatacatta
    11281 ggcgctcaaa agagtctgac ttgggataat ggtcctcggt tgatcgatat ggttactgag
    11341 acaaaaaaaa tcaaggatgc aaacaaaatc gtcatccatg cagaaaatca aacagtttcg
    11401 gtcatacgcg gattggattt tgagataaaa acgacttatg ataagacaga aaagccagaa
    11461 ataaaggaga aaaatggcgt gttgactgtg aatgcagtca aaaaatcaac aggtgcaatc
    11521 attagtttag aagacacaag tgcacaaatc actattgttg tccctgcaga tcatgaatta
    11581 tctgaattga caatcgaagg gaataatagt gcaattgata tcaacgaagt atcaatcaaa
    11641 aaagtgaata ctaagacaca tgatggctgg gtatcgttca atgaatttac aggaggaaca
    11701 ataactgcga aagatgaatt aggctcactt cgattagatg aggccaatgt ggataacttg
    11761 atcgtcacag gtgaaaacag caacttccat ctagaagaca gtttgatcag caaagaagga
    11821 aaagtcatcc taaacggtgg caatttatct ttgcatgaga tggaaaacag tggctatact
    11881 atccaaacgt ctggtgaatc ttatattgaa aaagactacc agccaataac aaatactttg
    11941 ttagaacaag gacagaaaaa gctaaagatc actgctaaag acacatcgat caatattacg
    12001 acggatcaag aggacaatga aacagattga tcctgtactc aactggatat tttaagaaaa
    12061 aacagcaaag atccttccaa tctttgctgt ttttcatcgc ttaacgcaca taactatagt
    12121 aaaataagaa agaaaatatc taaaaagaaa acagggtgaa agagtatatg aaggtagaag
    12181 aaggtcaaag agaaaagtta aaaacagaaa tggaaagatt acatacttat atcactcaac
    12241 taagccaaac cttctatgat ccagacaaag aaaaagtgat ggttaattac ccgaacaatt
    12301 cagaaggtcg ccaattagag caagtgtacc atgaagtttt caaacattta ttgacggtga
    12361 agaaagagct ggattattat tctttaccga ttatcgatac aggaatattg aagtatgatg
    12421 ggaagaagga acgatttatc tttaaatctg ttcgggaaga tttgcctctt tcagcaggga
    12481 tggatctaga ggtcttggta gaagactatt ttacagaaga aaagcactgg gttcgaacaa
    12541 aattggatta tttaccgcag gcagccggag ggacacaggc aagcggttgg tacatcactg
    12601 aagacaaaga gttagaacta gaaggtgtaa tggcgcgtat tcgaaaaaaa aattaaaaaa
    12661 gtaacattag atatttattg aaggataaag gagcgcagga agtagcatga gagaaaaaag
    12721 agcattttat atcaatggtt atgcagggat tgtcggattg gtcgtattag ctttagttgg
    12781 attgtttttc ttctatttag gtatgtggca agcgaaagtt tgggcacttt ttttaagtat
    12841 ttttctttgg ttgatcacgt tattgttgtt aagttcggca actgtagtca gtcctaatca
    12901 agcaaaagta attttgtttt ttggacaata tttaggtacg attcgagaaa atggcttttt
    12961 ccttacgata cctttggcac aaaaaatgac tgtttcgttg aaagttcgga atttcaatag
    13021 ttctgtttta aaagtcaatg atttggatgg aaaccccatc gaaatctcag cagtagtagt
    13081 ctttaaagtc gttgatacag caaaggcatt attcgatgta gcgtattatc aagattttgt
    13141 agaaatccaa agtgagacag ccattcggca tatcgctagc caatacccgt atgatacatt
    13201 caatgagaat gatttgacgc ttcgaggaaa cacgacggca gtgtctgacg agttgcaaaa
    13261 agagctgcaa gagcgtttag cagtagcagg tgtcgaagta atcgaaacac gcttgaacca
    13321 tttagcctat gcaacagaaa tcgctagtgc catgctccaa cgtcaacaag caaaagccat
    13381 tttgtctgct cgacaaacca ttgttgaagg agctgtcaca atcactcaaa tggccttaga
    13441 gcaaatcgaa gatggacaag aaatcaattt caccgatgat cggaaagtcc aattgatcaa
    13501 taatttactt gtctcaatca ttacagacaa aggaacgcaa cctgttatca atacaggcga
    13561 catgaaagac cgtaagtgac atactttttg taagatagga agaaggagtt taggacggat
    13621 tattgtcttg aactcttttt ttagttgttt taaagaaaat gacaaaattg aaagaagaat
    13681 gatagttgat tcgaaggcag aattgttcgc ttttttctat aatcagtata taaagtaaga
    13741 gagaagggga aaaagtgaaa aaacttctga tcggtctact tgttttcatc gttgtggtac
    13801 ttgcaggggt gaaaatcgca gattacgtag ttatgggtgg agaagaatac tatgtacaaa
    13861 ttacgacaga tggtacaaaa acagtttcaa aatcagactc tggacaagaa acagtcaact
    13921 atagatatat cctgcctggc tatacgaaag agggtaagga aaaagaactg gacttcaagg
    13981 gacaattgac tcgaccgctt cgtaaaggag catatttaaa aatcacctgg aacaaaaata
    14041 aaggtgtcac ttcgtatgaa gaagtcgaaa aaaaagacgt gccgaaaaca gcactggaaa
    14101 aactaattaa agggtaggga aagaaatgaa caaaatagtc gaagtacaga atgtaacaaa
    14161 agtatacgga aaaaacaatg agaaaaagac gcaagcatta agcggcatca gcttcgatgt
    14221 agaaaaaggc gaatttattg ggatcatggg cgcttctggt tctggtaagt cgacactttt
    14281 gaatatttta tccacattag ataaaccaac agatgggcat atccgaatca atcaaaaaga
    14341 tgtgaccaca ttaaaaggaa atcaactagc agattttcgt gcgaatgaga ttggctttat
    14401 tttccaagat ttcaatttat tagaaagctt gacagcacaa gaaaatatcg ctgttcctct
    14461 ttctttacaa ggcgtcagac caaaagaaat caagcaacgt gtccaaaaaa ttgcagaacg
    14521 tttatccatc tctcatattt tggaaagcta tccttctgag atttccggtg gacaaaaaca
    14581 acgggtcgcc gccgcacgtg cattgatcac acaaccaacg attctattag gggatgagcc
    14641 tacaggtgcg ctggattcca aaagtgcccg ggatctttta gatacgatgg acgaattgaa
    14701 tacaaaagat catgtatcga ttcttcttgt gacgcatgat ccgttttctg caagttattg
    14761 ccaacgtatt ttattcatca aggatggcgg tatccatcaa gaagtgaaac gtggagatca
    14821 aagccgtgat tccttctatc gagaaatact atctattctt ggaaacttgg aacagtaggg
    14881 ggagattgac tatgctttgg aaattatcac ttactgggat caagagccgt ttaagagact
    14941 acttggtgtt attctcagga ttagttatgg catctgctat tttttacatg tttgaatcaa
    15001 tggcaagcaa cgaagctttt ttgaaaagta atacgatcgt tgcggctaca gttatcattt
    15061 tccgattcgg ttctgtttta ctagggatca tcacattcgt ttatatttta tatgcaaatt
    15121 cctttttgat gacgatgcgg caaaaagact atgcaatgtt tatgatgctg ggagcaaaag
    15181 gcagaaaaat cgcacagatg attttttctg aaacattctt agtagggatc gcagctactt
    15241 tagtgggttc agctgtcggt gtaggattga caagtatcgt ccaccgatta ttagtggatc
    15301 agttagatct caatatttcg cattttacac cttttagtat tcacgggttg ttgatcactg
    15361 ttttgttttt tgctgttttg tttttattag ctgctgttgt caatgctttt tcgattgtca
    15421 aaaaaccgat tttagcattg attcgtgcgg atcagacacc aacaagaatc aagcagcata
    15481 aattcttgtt tttccttgaa gtagtactgg gaattgcttt tctaggctgc ggttactata
    15541 tgatgaaaca cgtggggacc tatcaagtct ttggtatagg ggtagcctta gttacgatcg
    15601 tattaggaac ttatttcatt ttccattctg tgatcatttt ctttttatca ttactaaaga
    15661 aatcagaaca gatttcattg aaaaaattga acaatttcac attgtcacag ttaaactttc
    15721 gtattcgtga atatacacaa atgctctcaa tggtcgctat gctgtttgcc ttggcactgg
    15781 gcgctttgac ggtaggatta ggttttaaaa atgaaattgc agacttgaca gaaaacaatt
    15841 ctggatatga tctagtgatg aataatcctc gggcagaaga tcagcaaaaa atcaaagaac
    15901 tttcaccaac actttctgct tcttatactc aaaaagaaga tgcgggaacg atttactata
    15961 atgcaagtga atttgcggct caaccattga tcatgataaa acatgaagat ggaacaccgc
    16021 cgaagatcac gaaagaaaaa gttcctgcta aaaaaatgaa tgaactgact gtacaagagg
    16081 aacttcgaag ctatgaattg ccggaacagc aagaaaaaga aatcaaggta gtcagtcaat
    16141 cagaatttga tcaactgaac ttgccacaat ccactttaca gctagttcag gtaaaagact
    16201 tccaagcaaa cctgaaacca atcgaaacac ttgttgctca aaataaaatg aacaatccat
    16261 ctttgcaaaa agtagagtac agcaaccaga aatatgaaat gtatgaagtg tataatggtc
    16321 tattctctgg ttttgaattt atgggattct tcctaggttt agctttcttg accatgcttg
    16381 ctagctgttt gatgttcaag atcttgtcag gaagcaagag tgacattgct cgttatgaaa
    16441 tgcttgaaaa gataggaaca cgacgcgggt tgcttaaaca atcgatccgt agagaagtag
    16501 gcgtgttatt cttagcacca ggaatcttag gtgtagtcca tgtcttattc ggattacaaa
    16561 tgtttggtct attcatgcaa aatccataca aagatatttg ggttccgttt acgatattct
    16621 tcgtattgta tttcatctac tatgtactga cgacatggtt atatactgga atcgtattga
    16681 aaaagagatt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa gcaaaaatag
    16741 ctcctcatat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
    16801 ttgttcggaa aaagaggttg tgacaatatt tttgtcacaa cctttttttc acggtctttt
    16861 attagaatat agtaaatttt ttctcgttac tcacggaata agctgttaag aaaacggttt
    16921 ccatgtatac tggaatcagt gcaaatgaaa gaaaagaggg tgcaaggaac atggaaatca
    16981 atgaacaaaa ttttggcgaa aactatcact tgattacgat cacgaatcaa aatgggacat
    17041 ctttgtctgt cagtgatctt ggggcacggg ttgttcattt tcaaacaata atcgataatg
    17101 aaaaacgaga gttgatctta ggttttgatg aagcaaaaga atatctagaa aaagaccctt
    17161 atatcggtgc ttcaatcgga cgtatggctg gacggattac gaatggcaca ttcaaattag
    17221 atgatgaaac gttcgaagta aaaacagacc cggacactgg tcattgttta catggtggtg
    17281 cacctagctt tgaagccaag cagtggcatt atacagtcat caatggtgaa aacgaagcat
    17341 cggtcatttt ctatcagact agcccagata tggaaaacgg atttcctgga actttagatg
    17401 tagaagtacg gtatacgctg acgaacgatg atacatggcg agtgacggtc caagggatca
    17461 gtgaccgcaa aacagtattc aatcctacaa atcatgtcta tttcaattta accggtgatc
    17521 cagcacagtc aatcgacgaa catatacttt ggttgaatag ccgattattt gctacgctca
    17581 ataaagatac aacgcctaca ggagaagtag tttcagtaag tggcacagca tttgacttcc
    17641 aacgtcctaa aaaattaggt gaggtctttg ccagtgattt tcgccaatca atgatagtgg
    17701 gcggcatcga tcatccattt ttgttgaaaa aacaatctgg tttactaaat gctgcggaat
    17761 tgacaagccc agatgaaaaa gtatcgatcc aagtaaaaac agatgcttca ggaatcgtga
    17821 tttttacagc aaattttgga gaagatggac cggaaatgcg cgggaataaa ttagtaaatc
    17881 atggcggtat cacatttgag actcaagaac tgccaggagc ggagcgactt gaaagttttg
    17941 gagatgtcac tctggaacct aaccaagtac gcgagtttgt aacggaatac aaaataaata
    18001 taaagaaata gaataaatta aaaagggagt gtgacagtgt cacatcccct ttttaattta
    18061 ttctatttct ttaaaggcag atcaagccag aatgatttgt ctaaccttaa caaggttagt
    18121 acaggaacag ttgcagcttt aactgtacga gtagttgttt ctggtatctg tcttgccgta
    18181 aaattgttcc acaaatccca gattgaagcg acacggaaga tggaagaagc gttgttgacg
    18241 cgttttatcg ttgaaaattc taatcctgtg attggcacgc catctgttgt ccatttgatt
    18301 ggcacatcga atcgcttaga catattgagg atttcagcgt ttgcttcttc agttgttgct
    18361 gcttcttttt tcggttcatc atattttgtc aggtcgtacg cttcgatgat cgcgttcaat
    18421 ttttgtgaat attggcgatc tgtagcataa cgtccttcca aatatttggt tgcttctttg
    18481 tagtcttttg tttccgactt ccaagttggt ttgtagaaat caggattgcc agtcagtcca
    18541 tttttgatca agtcggcata gtcttccaaa ctttccttgt aagaaggata tttacggaat
    18601 tgtgcgcgga tttggtagct ttgtccggaa ctatcttgtt caagtgtatt gaaggtcacg
    18661 ctgcttcctt tatatgatcc tttgatacca aaaaggttat gatttggttc gcttgataat
    18721 aagctgtttc cagatccact ttcaaggata gcttgagcca tcatgactga tgcgtagatt
    18781 ccttcttttt gcccgatttc ctgtgcatca tctgcaatct ttttcaagaa ctgatcagtt
    18841 gtttggtttt gactgaaatg gaacgagcca tctaatccat ctaaaataga tgagccgcct
    18901 gtcagcgaac ctgaagcagc aggtaaacct gctaagttta ctcggacagc aaaatcaggc
    18961 gtgaaatctg aaatagaagt tactcgaact ttatctcctg gttgaggcgc atggataaat
    19021 tgtccgttcc caatatagat acctacatgg tgagtaacac cggaagaagg tccccagaag
    19081 acgaggtcgc caggttgggc ctgacttact ggaatctgcg tacctgcata ttgctgttcg
    19141 cctgtccagc caccgatata atgaccggtt gctttcatat atacgtaata agtcaaacca
    19201 gaacagtcaa aagtatttgg accttttgct ccccatacat atggttttcc aagatgcttg
    19261 aatgcttctc gaacgattgc ttgttggaca ttcgttgtat ttgtaggcaa tacagcatca
    19321 attggtgatt ttacaacaaa atcagaagaa gcagaagatg gattctctat accgctttga
    19381 tttgctgtat ttggacgaga tgaagcattg ttcgttccgc cattcgatga accattgttt
    19441 ggatggtttg gttctctctg agtatttcca ttgttattcg atccagatgg gtttgtttga
    19501 ttcgaagaat tcccgctgtt cgaattattg ttattattgt ttgaattatt tgaaccatta
    19561 ttattgttgt tattacccac atctggtttg tttggcgttt ccggatctgg tgtactaggg
    19621 acttcttcac taggatcagt tggtttctct gttgaagtat ccggtccagc tttaaattct
    19681 gcagtaatag ataaattgtt cgtacctact gtaaaggtct ttgcatcata atttacttct
    19741 ttggcaccat taacagcgac tgaagcaatt tgttcacctt cagctggcgt aatagtgtaa
    19801 gttaccttcg tttccgctaa taaaccttct accgttccac tttccgtaat atccaaaggt
    19861 tgctgttcag gggaatccgc tgtcgaagca gtcagttgga tcttaccaat caaagaagga
    19921 gcaattgtaa gggaatatgt ctcggctggc ttttcatttt ctgatggatc agccggatca
    19981 atttcagttg gctcggtcga tcctgttgtt gagttagttg ttccagttgg gtcttctgta
    20041 aaagaatccg ttgtttcact agtagctgag ctggatgtag tcgtatctgt tgtgactgtt
    20101 tgatcagcta tttgattatc tacggcaaat acggtattgg tcgacaaaac agacggagca
    20161 atcagccaag tagcagctag caaacgtatc gtttttttat ttttcataat aaactagtcc
    20221 ttttcctttc attgattttc tgtcttattc agcattcatt agacattttt tggaacagat
    20281 aatcgtgtcg aaaagctttt tcttttcata aatctgtctg tgtattctaa ataaaacaat
    20341 ttgtaaataa gcaacgagca ttcattcgtt tttctttgac gttcatttta ttgcatagac
    20401 cacctttgtt tggggataaa caagtgggga taaaaatggt tatgcatatg taaattatac
    20461 caaagtttgg aaagtatcac aaaaatttat aaaaataatg atcctaaaaa gaaaaagact
    20521 gaaatatgtg ataaacatct catacttcag tctttttcat taactattca taagacatta
    20581 ggtcttcatc aaacccgcta acgagagtag tgatcattgc tacgactaga ctaacagcca
    20641 gtcctgctaa gtaccagaaa aaagtaccgg aaatgaatgc aggcaatgtg gtcagactac
    20701 caaatacaaa agcgatcgct ttcacttggg caaatcccat tacagctcca ccagcagctg
    20761 caccaatgca agcagcaaca aatggttttt tcaatgggat cgcaattccg taaacagttg
    20821 gttcggtcac tcccgcaagt aaaatcgaag caaaagcaga accggagatc gaacgggttt
    20881 ttgaacgttt acttcgcaga aatacaccta aagctgcacc agccattcca aaattggctg
    20941 ctcctgtcaa cggaacgatt cggtcgaacc cataagcaga gatattactg atcatgacag
    21001 ggtttacagc ccattggata ccaaatatta ccaaaatatt ccaaccacca gcgatgattg
    21061 cgccactgat cggtccgtta atatccatca tccaagtaat tgcggcagct aaaccttcgc
    21121 caaggtaaac acccgcaggc ccgaatacca ttaacgtcaa aggaaccgtg ataactaatg
    21181 aaagtaaagg tacaaatact aattgcatat tttcgcgaat atattttttg aggaattttt
    21241 caacaaaaga ataaaagaaa atacctaaaa tcgccggcat gacagtagac gtatactgca
    21301 tcagtactac aggaatcccc agaaaatcag tagctgcttt gccactgtct gaaagtaaag
    21361 aggtcagatt cggttcaatc agtgctgcag caatgacagc cgcaatatat ccattcactt
    21421 taaacgtttt ggcagcagaa aaagcaagaa agattggcag aaaataaaag acggcattcg
    21481 acgcagcaga caaaatatga taagttccgc ttgtttcact aagccagcca atcgtagtcg
    21541 ctaaaacaac cagacctttt aatataccag aaccggcaaa cagcggaatg atcggcgtaa
    21601 agatttccga tatcaaacca aatagttttt cgatcggatt tagcttttct ttcggagctg
    21661 aatgatcgat ttgacgcgtc aattccttgt aagcaggagc caatccctga tccatgatta
    21721 tttctgcata tctatcgagt aaaatgaaat acttaatgga agggatttgc tgttttaatt
    21781 tttctgcatt ggctactggc tgttttttca atttgatacg aagccgagtc gcacatggaa
    21841 ggacagaatc aatattgtct tttcctccca actgttcaat cagcatatga atctgctcgg
    21901 acagtaccat ctgttcagga ttcattgtcg tttccattag aagaccactc cactcactaa
    21961 tataatattg gcgtaaggtg tcatttcgcc tgtacgaata aatgcatgac agttattcaa
    22021 ctcttttttc atttcttcat gtgggataaa gctaatcggt gtatctggta acaataattt
    22081 gattttttcc aatatttccg ggttcattgt ctttatttct tccgccaatt caatcgattg
    22141 tacctcaagt tcttctaaaa cgttttctaa aacagataaa aaacttggaa gaccgtttga
    22201 gacagccaaa tctatttttt cagtaccaaa ggggacaggc atgcctgcat ccccgatact
    22261 taatttatca aagtgcccca tttgtgcgat tactcgtgaa atatctgaat tgattacttt
    22321 tccttttttc atgtttgttt cttcctctca ttgttaattt tagactgctg attgttctaa
    22381 agcttcgcgg taagggatag aaggttgcgc accataagac tgcacagcaa cagaagaggc
    22441 tagatttcca taaaggatcg cttctttcaa gttactaagg tctggctgaa gtgtgctgct
    22501 taatgcgcca ataaatgtat cacctgcggc tgttgtgtca acggcatcca ctttaaaagc
    22561 agggacgata ccgtgttctt tttctgtatg atagaaagcg ccggcactgc ctagtgtaat
    22621 gattactgtt ccaataccta attcatggag tttttcggct gctgcgacca aactgttatg
    22681 atctgtcact ctgattcctg taatgatttc tgtttctgtt tcatttggta taatgatatc
    22741 tgtcttttca agtagttcag taggaatcgt ttcacgagct ggtgcaggat tcaggatcgt
    22801 tgtttttcct gcgtcttttg caatagaaaa agctaggatc gtcgcatcta agctactttc
    22861 aaactgagca ataataaagt cactgtttgt aatcagttgc ttgtttttta ggacttcttg
    22921 ttcatggaaa gcattatttg cccctgcatg gatcaagata ctattttctc ctgctgcgtc
    22981 taccataatc attgcttgtc cggttgtggc gccttggatt tcagctatcc cagatgtatc
    23041 gatgttttct tttgtcagta gatctaataa ctgttgtcct tcgctgtcag ccccaacgcc
    23101 tccaataaaa gatgtccgtg cgcctgaacg tttggctgcc actgcttggt tggcaccttt
    23161 acctccgcca gcgtgaaaaa tctcatgcgc atgcatcgtt tcgcctggct tgggcatgtt
    23221 ggttaaacga agggtagtgt ccatattaat acttccaatt actgtaaccg tgttcatttt
    23281 acaaacctcc aactatctca tttattttga tgggaaaatt attttttaaa acgttttagt
    23341 aaaacgtttt aatagttcga taaaaatata gcacaccttg aaagctattt caagggtgca
    23401 gttgaaaatc ttttttctaa tcgtacaggt aatctttttt cttcccattc tttttctggg
    23461 aattggatcc gatctaacaa taacttggca gatgtttgtc ccaattcgaa gattggttgt
    23521 gcgatcgttg tcagtttcgg cttgatatac tcgcacatat ctatattatc atagccaata
    23581 atgctgtagt cttcagggat gctttttcca gcttcctcta atccgcgata taatccaaaa
    23641 gctaattcat catttaaagc aaacacacca gttgcagcag aatcaagaag ttctgctgtg
    23701 atctggtagc caccttgttt tgagaactgt gtaggtaaga ggatcagttg gtcgtgaggg
    23761 atttggtaaa catcaagagc agatttaaac ccctcaatcc gagcgtggac attttccggt
    23821 ggattttcag gatagacaag ggcgatcgtt tgatgtccta gactaagcaa gtgcatgccc
    23881 gctaggtaac caccgcgaaa atcgtctgtt cgtacggcat cactaaatcc ttcagatttt
    23941 ttctgatcca aaacaatgaa gggccggcct tgttttttca aattttcgtt gattgcatct
    24001 gtagagacag cagaagtcgc gataatgaat ccatccacac ctcgacgtgt gagctcggct
    24061 agatattcga tttctttttg atgatcagaa tcagcattac ataatatcgt gacgaaattc
    24121 tgcttataca agatatcctc aattcctcgc atcagtgtac tgaagaatgg attcgtgata
    24181 tcaggaacga gtacaccaat tgttttcgtt tccttggtaa tcatttgtct agcaaaatag
    24241 tcaggctgat agcctaattc ttctttggca gctaaaactt tctgaacagt tttcggactg
    24301 aatttctccg tatttccatt taatatcaag gagacagttg tgattgaaac gccggaaagt
    24361 ttagcgactt ctttgattgt gatctttttt ttcaccatgt tcatactcct atctaaatcc
    24421 tgtggttatt ttcccatgaa atcaaaaaga agtaaatgaa aatcacgtgt gccagtactt
    24481 atttgctttg ttggatagct tcgattattt gtttcgttga gcgtactcgt gcagttttcg
    24541 gaaatatctt ttttatgaac aattgatggg attctttgtc acgatcactg caagcatctt
    24601 cagcaagcac gatatggtac ccgtgttgaa aggcatctaa tgcagtagca tatacaccgt
    24661 ttgttgtcgc tacaccgctt aaaatgatag tttcgattcc acgccggcga agttgaagat
    24721 caagagaagt accaaaaaaa gcacttggat tgtattttgt gatagtcaac atattatctg
    24781 tatctttcaa agaatccttc aaaagtaaat cagtgaattc tggaggtaca gaagcagtca
    24841 ttccacccat atcagtttgt tgactcaaat aagggaaagt ggagatgtct acgttcacta
    24901 aaactttcaa ggcagcggta cgtttcaatg ttcttactaa caaatcattg tttgtcagaa
    24961 tagatgctgc agtgtgtgga gagagtgtac cgttatttac gatctctttt tgcatgtcga
    25021 tcgcgacaaa agcagttttt gataaatcaa aaatcatatt ttcctttaaa tcattcatga
    25081 tgttccttct ttcaaataac aatgtttttt atgtaagtaa atcataaatc gtttatttgt
    25141 caactaaatt aaaggattcc tttctttagt ctagagaaat tcagtggtaa aataaataag
    25201 tgtgagaaaa cgcattcgat gcatagatat agcgagaaat atcgtgaaaa tggaggaatc
    25261 agagtgaagt atgtcgaagt aaaaaatgaa tacaaacgat acaaaatggg agaaacgacc
    25321 attacagcaa atgacgatat ttctttttca attgaaaaag gcgaattagt gattatactt
    25381 ggtccaagtg gagcaggaaa gtcaacgatt cttaatattt taggtgggat ggatacacct
    25441 gatgaaggtc agatcattat tgatgacaca gatattgcgc aattttcaga taaacagctt
    25501 actgcttacc gacgaacaga tgtagggttc gttttccaat tctacaatct cgttcctaat
    25561 ctaacagcaa aagaaaatgt ggaattggca acggaagttt ctcctgatgc gcttgatcct
    25621 gtagaggtat tgaggcaagt gggcctagct catcgattga ataactttcc gtcccaatta
    25681 tcaggaggcg aacagcagag ggtttcaatt gctcgtgcac tagctaaaaa tcccaaactt
    25741 cttttatgtg atgaaccgac aggcgcgctg gattttgaaa cgggcaagca agtattgaaa
    25801 ttattgcaaa acgctagcag acaacacggc aataccgttt tgatcattac acataattct
    25861 gctttagcgc ccatcgctga tagagtcatt cacattaatg atgctaaagt aaggtctgtt
    25921 gaattaaatg atcatccttc atccattgat gagattgtat ggtaggggga ttttatgaaa
    25981 acaagaacat atcttaaagc aggttttcgc gaaattcggc aatctaaagg acgattcatt
    26041 gcaatcatcc ttattgtcat gctaggaaca cttttatttg taggtgtcaa aactgctgga
    26101 cctgtcatgc aaaaaacgat ggatcactat gttggtacag caggactttc tgatttgcaa
    26161 atcgtttcta ctggtggctt gacacaaaaa gatattgctc aagcagaaaa gatcccagat
    26221 gcaaaagtag aaacaggaaa acagatctat tattccaata ctggcaaaaa cgaagtgatt
    26281 caaatatttt cttataacaa aaataacaag cagaacaagc tacaattaac tgatggaaaa
    26341 ctcccggaaa aaccaaacca gcttgtattg gatgaaaaag cggaagatga agggtataaa
    26401 atcggtgata tttatcagat tgattcagat gaattgaaag aaaaagagta taccatcacg
    26461 ggctttgtcc gttctccatt gttcatcaac aatttggaga gaggatacgc gaacgtcgga
    26521 aatggtagcg tcgattattt tgtctattta cctgaatccg tttttaaaac agatatttat
    26581 tctgtcttat atcttgattt tacaaacgta aagaatttga atacgtacag caaagcttac
    26641 aaagataaaa tggagcaaaa tcaagaaaag gcagaaaagt atttacaaga tcgacccgaa
    26701 gaacgcttag aagagctgaa aaaatcggcc aacgaagaat tagaaccggc acagcaaaaa
    26761 atagcggatg gcaaagcaca aacaacgcaa gcaagaacac ggttagaaga tgctaaaaaa
    26821 cagttagcgc aacagtcagc aactatccag cagcttcctg aagcgcaacg aacaatcgct
    26881 gttcaaacat tatctcaaca agaagctcaa cttgctgaac aagaaaggca attagttgag
    26941 aaagaacgtg aactggcctc tgcagaggaa gaattgaatc gaaatgcaga aaaaattgaa
    27001 aatttaacta aaccatcata cctatacaat gaacgttcag aaaatgtcgg ttttcaagaa
    27061 tttggtgatc tgtctgatcg tatcgctgcg attgccaatg tttttcctgt tttctttttc
    27121 tttattgccg cattgatcac ctttacgaca atgacacgta tggtagaaga aaaccgcagg
    27181 gaaatcggga cattaaaggc gttaggttat acgaaagttg aaatcgcaaa aaaatatgca
    27241 atctatgcaa gtctagcctc agggataggt attgttttag gaacgatatt aggaacgaat
    27301 cttttgccaa ggatcatttt tgagctgtct aacgagcggt atgatattgg gagtgcgctt
    27361 attttttatg actggccgcc gattttacaa gcagctgccg cattcttcat tgccgcattt
    27421 ggcgctgcaa tgatcgtttt attcaaagat ttaagagaac ggcctgctgc attgcttcaa
    27481 ccgaaagcac cgaagccagg gaaacgcatt tttcttgaat acatcactcc gctgtggtca
    27541 cgccttagct ttaatcaaaa aatcagttac cgaaatctct tccgatataa gtcaagaatg
    27601 cttatggcga ttatcggaat tgctggatgt gccgggctga tggttgcagg cgtgggattg
    27661 aaagattctc taagttctgt ttcagccaaa caatttggac cgatcattga ctatcaagca
    27721 atcgttacat tggattcatc cacggaagat agtcgtcaaa aagcagcgga tatgctgcag
    27781 aagcaagaaa aagtaaatga gatcctagct attcatactc aaaccgtaga attgagaaaa
    27841 aaaggacatg cttcgcaaag tctgacagtg atcgtaccaa gtaaaacagc tgatttggat
    27901 cgttttatcc atttaactga tcttgaagag aacccacgct ccttgcctaa aacaggaatc
    27961 gctattacgc aaaaagtagc agaactattt gacttatcta caggagatca tatttcgctt
    28021 tatgatgaag atcaaaaaga atggaaagtc gaagtaaaag aaatcgtgaa aaactatcta
    28081 ggtagcttta tttatatgtc ccctgaatat tatgcttcag ttactgatca atcgatgatg
    28141 gataatgcat ttttgctgcg tacagataag atgacaacaa aagaagaaga aatgctttcc
    28201 gagaaactgc ttgccacaga cgctgtgaca aatacgtctt tcatgtccaa acaaatcgaa
    28261 acgcaagaag aatcaatggc gaacttagac tcggttgtct taatctttgt cgtattatca
    28321 ggactgctgg cttttatcgt cttgtataat ctaactaata tcaatatttc cgagcgtgta
    28381 cgcgaattat ctacaataaa agtccttgga ttttttgata aagaagtcac gatgtacatt
    28441 gttcgagaaa atatcatctt tactctttta ggaatcatcg gaggatttgg tgtaggatat
    28501 atattgactg acttcatctt gcaacaagcg tctatggaga atgtcatctt tccattagtg
    28561 atcacatggt gggcttatgt cttatcagca ggattaacta tcgtatttac ggttattgtg
    28621 atgattgtta cccatttcaa attaaagcat atcgatatga ttgatgcgtt gaaatcgaat
    28681 gaataaaaac agaggttgtg aaatcagcgg tttgacctga gtattagaga aacaaacggc
    28741 aaaatagttt ttcatatttt gccgtttttt gagctaatac cgaaggacag ctgattgaac
    28801 accatgaatc agaggttgta aaaaagccgt cctgcatcga gcaaaaagaa tactcaaata
    28861 aaattagctc ctcatgtttt acatttgaga agactgattg ccgtagataa aagcttttga
    28921 acaccattta tagaagaaac aagaatattt tgctatttta aacaagagga tagacaaata
    28981 catgtctgat ctcttgtttt ttattgggca acagacctct tagaatgtat aaagataaat
    29041 tttacaaacg ctttcggttc agttataata ataaatgata acttgtatat ataagttgtg
    29101 gtaggtaata aatcagttga atggaggtaa atatccatga catttaaaga caaagtcatt
    29161 taccaaatct atcccaaatc ttacaaagac agtaatggtg atggaattgg agatttaaaa
    29221 ggaatcaaag aaaaactccc gtatctgaat gatctaggca tcgacatgat ttggctaaac
    29281 cctatttatc caagccctca aaaagacaac ggttatgata tctctgatta cacagccatc
    29341 gatcctatct ttggtacgat ggaggaattc gaacagctaa tagccgaagc aaagaaatac
    29401 cggatagaaa tcatgctcga tatggtgctg aatcacgttt cgacagaaca tgaatggttc
    29461 aaaaaagcac tagcagggga ccagcattat caggacttct tcattttaag agaagagcca
    29521 acggattggg tatcaaaatt tggtggaagt gcttggtcgc catttggaga tacggggaaa
    29581 tattatttgc acttatatga tcggacacaa gctgacttga actggcggaa ccaagcagta
    29641 agagaggaac tattcaaagt tgttcgtttt tggatggaaa aaggggtaaa aggattccgt
    29701 ttcgatgtta tcaatgttat cggtaaagac gaacaattga aaaataataa agaaaatgat
    29761 ggcaaacctg aatatacgga caaaccaatc acccatacgt atctaaaaga attgaaccaa
    29821 gcaacgtttg ggacggattc cactatcatc acagtcggtg aaatgagctc tacgacgatc
    29881 gaaaattgta tcttgtatac agaacctagt agagaagaat tatcaatggt ctttaatttc
    29941 catcatttga aagtcgatta tgaaaatggg aataaatggt ctactcctcc atttgatttt
    30001 gcagaattaa agcgactatt tcatacatgg ggagaaaaga tgagtgaggg taacggctgg
    30061 aatgctctat tttttaataa tcatgatcag ccacgtgcgc tcaatcgatt tgtagctatc
    30121 gatcagtatc gaaaacaagg agcagagatg ctagcaacca tgatccattt gaaccgtggg
    30181 acgccctata tttatatggg agaagaaatc ggcatgatcg acccagacta ttcatcgatt
    30241 tccgactata ttgacgtaga aagtttgaat gcttataagt tactccttga acaaggattc
    30301 acagaaaaag aagcgtttcg tcggatcaaa gcaaaatctc gcgataactc tcgaacaccc
    30361 atgcaatggt cagacacaga acaagcagga tttactacag ggaaaccttg gttaaaagtt
    30421 ggaggcaagc tagaagagat taatgtagaa aaagaactat cttctgaaga ttctattttg
    30481 tcctattaca agaaattgat tcggctgaga aaaacatatc caatcgttgc acaaggagat
    30541 tatcatgctt atgaagcaaa tcatccggaa gtttatggct ttcttcgcca atttgaagga
    30601 cagcagctgc ttgtattaac aaatttctat gataaagata caaggatcgc catcccagaa
    30661 ggattcacag aagccaatat tcttatcgat aattattccg atactgttct tcatcaaagt
    30721 ctggagctaa aaccatacca ggcaattgct ttgctgaaaa attaaaacaa aaacacaaaa
    30781 cgtcgttcct acgagctatt ctcgtaagat cgacgttttg tgttttattt aaaataatct
    30841 tcttcagaaa atgaagagtt ttcatactct cctttatcgc cataaaattt attgtagcgt
    30901 tgtttgaatt tacggaaaat aaaagtaacg agtaatttga ctaacgtata gataggaata
    30961 ccaaaaataa cgcccacaat tcccattaga tcacccatga caagtaaaac gattaagatc
    31021 gtaatcggat ggatctgtaa acgatcaccc attactcgag gaatcaccaa gtcaccgtgg
    31081 agaagttgta cggcaaacca gacaatgaca aatttgacag ccataaaagt agagtcttgg
    31141 aatgcaatga tcaaacctgg aaagaaggcg ataaacggcc caatatatgg aatgatacat
    31201 aacaacccag cagaaatagc taaaacacta gcaaattcta atccgatcag taagaaacaa
    31261 acccagtaga ctgctccaag gataaatgaa gcaatgattt gcccctttag aaatgaaccc
    31321 aattgttgat tagcaatctt tgtcaaattt ttaaaatctg tacgaaatgc tggtgggaca
    31381 atcgctaaaa ctgactgata aaatttttga ggatctctca aaaggaaaaa agcgacaatt
    31441 ggaccagtaa ataaagtaat aaaaatagct gtaacagttg aaaaaatgtt gctgatccgt
    31501 tgtgcgccac tttcccaata atttccaata aatccagcta tatcagacgt cacgtcgtca
    31561 atagaagcga aaaattgatt aaatgaatcg gcaaaaggtg ttttctccaa aaattgtcgg
    31621 agactctctt gaaaatcttg aaacaaatct ggcaaactac tgatcaatgt ctttgcctgt
    31681 tcttcaagaa aaggaataag ccatatgctt ccaagcccca ataaaaataa aataatcaca
    31741 taaacgataa tgactgacaa gttcctcgaa aggtgcttgc tcaaacgttt gatgactggt
    31801 tccaaaagat aatacaagat cagtgcaaac aataatgggg gcaagatagt agcgataatg
    31861 attccgacag gcttgaatat aaacgagatc tgatggaaaa aataaatgat cagcgcaagt
    31921 aagataacta ttcctaaaac atagtaagag gtttttcctc ctaaaaagcg gatcaaacga
    31981 ttcgttgtat ctgtttcttt tttcactctt tttcctcctt tatctataac ttcattatag
    32041 acaaaagcgg gtataaaagg aaaatgatag aggttgattg caaaaatatc gtcaatcatt
    32101 catataaact ttctcatcaa ttaaaataag aaaacgcttg acagttcttt taaccatatg
    32161 ataaattaaa tcctgttgct aaaacgtaac tattacggtt tgcgaaaagc gaatcggaac
    32221 attcaaatga aagaagatga gaagaggagt gtcataaatg aaacgattga taggtatatt
    32281 ggccatgctg gttttagcag gtatgttaac tgcttgcggg gcaagtggga aagccgagga
    32341 cagtaaagaa aaattgagcg tgatgacgac cttttatccc atgtatgatt ttacaaaagc
    32401 aatcgtcggg gatgaaggag aagtagagtt attgattcca gcagggactg attcccacga
    32461 ttatgaacct tctgctaaag acatggcaaa gatccaagat acagatatct ttgtttataa
    32521 tgatgaaaac atggaaacat gggtccccgc aatccaaaaa acattacaag aaggcaatgt
    32581 ccatacgata aaagcgacag aaggtatgct tttacttccg ggaagtgaag aaggacatga
    32641 tcatgaccat gaacacgggg aagaaggcca tacacatgag ctagacccac atgtatggct
    32701 agctccaagt ttagcaatca agcaggtagc aaacatacgt gatcagctga ttgaagctta
    32761 cccagaaaaa caggaagtat ggacaaaaaa tgcagcagcc tataccgaga aacttcaagc
    32821 actccatcag ctttatcaag aaacctttaa gcaagcaaaa caaagatctt ttgtcacgca
    32881 acatgctgca ttcaactatc tagcgttaga atatggattg aatcaagtat cgattgctgg
    32941 tctttcttct agtgaagaac cgtctgcggc gagaattgct gaactgaaac actttgttaa
    33001 agaacatgga atcaactata tttattttga agaaaacgcc aaagatagta ttgccagaac
    33061 gttagcaaac gaagcaggtg tatctttaga agtattgaat cctttggaag gattgacgaa
    33121 tgaacaaata gaaaatggag aaaactatct ttctatcatg gaagcaaacc tcgaagcgtt
    33181 gaaaaaaacg acagagacag aaaacccgct agaagatact ttgaccccca aaaaagaaaa
    33241 gagcgtctac aacggttatt ttgaagacga ggatgtacaa gatcgctctc tttcagattg
    33301 ggcaggaact tggcaaacag tagatacgta tgtagaagat gggacttttg atcctgtttt
    33361 tgaatataaa gaaaaattga atcaggataa gacagcacaa gaatataaag aatattatac
    33421 gaagggctat caaacagata ttgcttcaat caagatcagt gagaaagaga tggcatttac
    33481 gtttaagaat ggaacaacga aacgttcaaa ttatcgttat gtagggaaag aaatcttgac
    33541 ctacgaggca ggaaaccgtg gcgtaaggta tctattcgaa gcagaagata aggaaagtgg
    33601 cgcttttaga tatattcaat tcagtgatca cacgattgca ccggctgact ccgaacattt
    33661 tcatttatat gcaggaaa
//
LOCUS       DADVXU010000018        32269 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.019, whole genome shotgun sequence.
ACCESSION   DADVXU010000018 DADVXU010000000
VERSION     DADVXU010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 32269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.019"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(73..1014)
                     /gene="ribF"
                     /locus_tag="KB953_000856"
     CDS             complement(73..1014)
                     /gene="ribF"
                     /locus_tag="KB953_000856"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HBB6865852.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            complement(1018..1941)
                     /gene="truB"
                     /locus_tag="KB953_000857"
     CDS             complement(1018..1941)
                     /gene="truB"
                     /locus_tag="KB953_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBB6865853.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            complement(2309..4671)
                     /locus_tag="KB953_000858"
                     /pseudo
     CDS             complement(2309..4671)
                     /locus_tag="KB953_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            5119..5292
                     /locus_tag="KB953_000859"
     CDS             5119..5292
                     /locus_tag="KB953_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBB6865854.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            complement(5449..5796)
                     /gene="rbfA"
                     /locus_tag="KB953_000860"
     CDS             complement(5449..5796)
                     /gene="rbfA"
                     /locus_tag="KB953_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBB6865855.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(5823..8129)
                     /gene="infB"
                     /locus_tag="KB953_000861"
     CDS             complement(5823..8129)
                     /gene="infB"
                     /locus_tag="KB953_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBB6865856.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(8142..8453)
                     /locus_tag="KB953_000862"
     CDS             complement(8142..8453)
                     /locus_tag="KB953_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HBB6865857.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(8450..8743)
                     /locus_tag="KB953_000863"
     CDS             complement(8450..8743)
                     /locus_tag="KB953_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HBB6865858.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(8765..9940)
                     /gene="nusA"
                     /locus_tag="KB953_000864"
     CDS             complement(8765..9940)
                     /gene="nusA"
                     /locus_tag="KB953_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBB6865859.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(9963..10436)
                     /gene="rimP"
                     /locus_tag="KB953_000865"
     CDS             complement(9963..10436)
                     /gene="rimP"
                     /locus_tag="KB953_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBB6865860.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(10581..14939)
                     /locus_tag="KB953_000866"
     CDS             complement(10581..14939)
                     /locus_tag="KB953_000866"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HBB6865861.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            complement(15151..16860)
                     /locus_tag="KB953_000867"
     CDS             complement(15151..16860)
                     /locus_tag="KB953_000867"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBB6865862.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            complement(16928..18196)
                     /gene="rseP"
                     /locus_tag="KB953_000868"
     CDS             complement(16928..18196)
                     /gene="rseP"
                     /locus_tag="KB953_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HBB6865863.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(18357..19157)
                     /locus_tag="KB953_000869"
     CDS             complement(18357..19157)
                     /locus_tag="KB953_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBB6865864.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(19154..19966)
                     /locus_tag="KB953_000870"
     CDS             complement(19154..19966)
                     /locus_tag="KB953_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HBB6865865.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(20161..20718)
                     /gene="frr"
                     /locus_tag="KB953_000871"
     CDS             complement(20161..20718)
                     /gene="frr"
                     /locus_tag="KB953_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBB6865866.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(20721..21443)
                     /locus_tag="KB953_000872"
     CDS             complement(20721..21443)
                     /locus_tag="KB953_000872"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBB6865867.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(21579..22460)
                     /locus_tag="KB953_000873"
     CDS             complement(21579..22460)
                     /locus_tag="KB953_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBB6865868.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(22559..23341)
                     /gene="rpsB"
                     /locus_tag="KB953_000874"
     CDS             complement(22559..23341)
                     /gene="rpsB"
                     /locus_tag="KB953_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBB6865869.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            23700..24179
                     /locus_tag="KB953_000875"
     CDS             23700..24179
                     /locus_tag="KB953_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HBB6865870.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            24396..25487
                     /gene="phnW"
                     /locus_tag="KB953_000876"
     CDS             24396..25487
                     /gene="phnW"
                     /locus_tag="KB953_000876"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HBB6865871.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            25480..25608
                     /locus_tag="KB953_000877"
     CDS             25480..25608
                     /locus_tag="KB953_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HBB6865872.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            25612..26157
                     /locus_tag="KB953_000878"
     CDS             25612..26157
                     /locus_tag="KB953_000878"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HBB6865873.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(26611..28302)
                     /locus_tag="KB953_000879"
     CDS             complement(26611..28302)
                     /locus_tag="KB953_000879"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBB6865874.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(28722..29669)
                     /gene="arcC"
                     /locus_tag="KB953_000880"
     CDS             complement(28722..29669)
                     /gene="arcC"
                     /locus_tag="KB953_000880"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBB6865875.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(29784..30803)
                     /gene="argF"
                     /locus_tag="KB953_000881"
     CDS             complement(29784..30803)
                     /gene="argF"
                     /locus_tag="KB953_000881"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBB6865876.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(30894..32123)
                     /gene="arcA"
                     /locus_tag="KB953_000882"
     CDS             complement(30894..32123)
                     /gene="arcA"
                     /locus_tag="KB953_000882"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HBB6865877.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         8599 a         6927 c         5728 g        11015 t
ORIGIN      
        1 ctgggcttat ttatcaccca ttttattgta attgctagtt atacataaac aagagatttt
       61 atgcataatg ttttattgct gagaaaaata ttcagctgtt gcttgttcat cctccttgag
      121 ttgttcaatc aattcctcta cactagaaaa tttcacttga tctcttaaaa attgattcca
      181 gcgtaccgcg acatgttcac catagatatc ttgattaaag tccaaaatat acaactctac
      241 agtcagctcc cgtccttcac cgaatgtatc attatgtcca attgaaccca tggctggata
      301 ccatgtttcc ccaattttta tttccgacac ataaacacct tctttaggca aatgaaccgt
      361 gctctttact ttgacatttg ctgtagggaa tcctagtagt ctgcctcgtg catcgccatg
      421 gacaactacc ccttctacct catatgtgca acctaatagt tttgctgctt cttttacttg
      481 tccgctgtct agtaatccac gaatacgggt agagctgatt ttttcacctt gttcttcttc
      541 tttagaaact gtgacgattt tgaatcttcc tttggcatat tcaggaaaat gagcgacatc
      601 tgcaatgtct tttggtccat acgtataatc aaaaccggaa acagcatatt ttgcatgtaa
      661 ccctacgatg tattgatcaa caaattcctg cggtgctaaa tgagcaaaag aagaagtaaa
      721 atcaacttca tataaataat ctatacccaa agcggccatt ttttcttctt tttgcttaag
      781 gctagtcaaa tatttcacgc tgctaggatc gatcttttta aagacaatag atggatgctg
      841 attgaaagtc atcaatgcta atttcaatcc ttctttttgt gcaatttcca tacctgtctt
      901 tatgactttc tggtgtccga tatgcacgcc gtcgaaaaat cccagcacta aaacgacatc
      961 gtcttctgga atttgttctg attggtaagg atgtctgatc ttgatgatat tcattcgtta
     1021 ttcctcattt ctcaatactt tactaggttt taatttgttt ttttctttcg ggtttggctg
     1081 ataaatactg acaacttttc ctttataaaa caaagcaatt tcttctgacg gcatttctgg
     1141 gagcgcaaat acagtataat caagtctcat accgtttttt actttctgcc atacttcatc
     1201 agtaatatct actcgtttga accgttccac tcctttttct attggcagca aacaatcatc
     1261 gattgtccca tttttcacag cttgagctac ttcttctaat gtcagagcat ccttctggga
     1321 cataccggca cttgctgttc gggtcagatc agacatgtgc gcagggtaac caagtttctt
     1381 gcctgtatct acagctaatg tgcggacata ggttcctttc ccgcattcta cttcgaagcg
     1441 ccaagaaatg gttcctgtca cttcgtctct tgtcaattca cttgtccgga taaaacggta
     1501 aatctgagcc tttctaacag gacgctccac tgtttcatta ttgcgggcat attcatataa
     1561 ccgcttaccg ttgactttta ccgctgagta cattggtggt atctgggtga tctcaccaat
     1621 aaatgaagcc atcacttcat ctattttttt ttcagataaa gacatatcta ctggttttgt
     1681 ttctacccgt tctccggatc gatcttccgt cgttgtactg aaacctagag ttatctcgcc
     1741 tacatagatt ttcccagaat ctgtcaaata ttcgataact tttgtggctt ttccaataca
     1801 aatgggcaac acaccgtcta cgtctgggtc aagcgtaccc ccatgtccga tttttttcgt
     1861 atgtaagatt tttcttaatt tgaatacgca atcatgactt gtcattccgc gttctttcca
     1921 taaaggtaat aacccgtcca tgtcttttct ccatttcatt tttgatttaa ctgaattatt
     1981 ataacacatt gcatcaatta ctgacgaatt tgacatcata atgaacggtt tttatttttc
     2041 ggcaataaaa acgtaacaaa attcatgctc acgtaaatag aaatcatgtt atgctttttt
     2101 aaaggaggga atagaatgga agattttttc tttgcaccat ttgattggat ttatgggtgg
     2161 cttacaggta aatagtttgg ttttcacttc ccaaaaacga aaaaaaagac tggccattct
     2221 tccagtcttt tttccgtttt ttttatttat tttttcagtt tcttaatcaa tgtaaaaaat
     2281 tctattttgc gggatcgatt ttatcatttt attggtcaag atttttccat ttccaatcca
     2341 atacttctgg cagatcaata ccattttcac gaatatagtt ttgatgttct tgtaatttct
     2401 gatccatttt tttcgcaaaa tcttctgctt gacttccttt gatttttaaa gctgcgtctt
     2461 ttgccaaatg ataacgatca agttcattta ctacacgcat gtcaaatggt gtcgtgatat
     2521 ctccattttc tttgtagcca tgaatcatta aatgatggtt atgacgatca aagaaaatcg
     2581 tgcggaccaa tgtttcataa ccgtggaaag caaataagat cggtttgtct tttgtaaaat
     2641 attgatcaaa ttcttcatcg ctcaacccac gcggatcttg acttggatga cgtaatttca
     2701 ataaatctac gacattgatg aaccgaattt ttagttttgg gaattcttcc agcagcaaag
     2761 ttacagctgc taatgcttct aagttagatt ccgttcctgc agcagcaatg accaattcag
     2821 gttcctcctc ttttgctgtc gaagcccaat caatgatttt caagccttca ttcactaaga
     2881 cggctgcttc ttctgctgag taaaattgag cacgcggatg tttggaagag atgatcaaat
     2941 tgattttttc tgacgagcgg aatgctttat ccatgactgc gagcaatgta tttgcatccg
     3001 caggcagata ttcacggatg aactccgcct tcttttctgc caaatgtgtc aatattcctg
     3061 gatcttggtg gctatatcca ttgtgatctt gctggaagac tgttgagctt gcaatgagat
     3121 ttagcgaagg ataatcattt cgccaagaaa ggtcatgtga tttccgcatc catttgaagt
     3181 gctgagtcaa catagagtca accactcgca agaatgattc gtaacttgca aaaaaccatg
     3241 ccgtccagtc agaacgtacc cttctaagaa cccttcatct tggtgttccg ataattgact
     3301 gtcgatgacg cgaccagatg gcgaaagcca ttcatcattt tctggttcga ttttttccat
     3361 ccattggcga tttgtcgttt tgaaaacttg attcaatcgg tttgattttg tttcatctgg
     3421 tccaaatata cggaaatttt ctggattttt ctcaatgatc tcgcgaacga atttaccaag
     3481 ttcggtcata tcttctgctt taacggagcc tggtttttca aattgcaaag tatatttttt
     3541 ccaatctggc ataataagcg gtcttggatc gattccacca ttggtgatag gattttttgc
     3601 cattctttgg tcaccagtag gcgcgatttc tctgatttct tctaaaatac gtccttgtgc
     3661 atcaaataat ttttctggtt cataggattt cagccaacgg agtaaagcat ctgcatgatc
     3721 catatgttct tgatctacag gaatcggtac ttggtgtgct cggaaggttc cttcaattgg
     3781 ttccccgtcc cattcttttg gaccggtcca tccttttggt gtgcgtacga tcaatactgg
     3841 ccattttggc atcacgacat ctgttgctgt cttcaggcga gcttcttttt ggatggattt
     3901 aattttctcg attgcttgat ccattttttc agccataaca ggatttaatt tttctgaatc
     3961 atttccttca ataaagaaag gttcccagcc aagtccgtta aagtaatttg ctaattctcc
     4021 atctgattta cgtgccaaga tcgttgggtt ggctattttt gcaccattta aatgaaggat
     4081 cggtaaaaca gctccatcta tcactggatt gataaagaca tttgagaacc agcttccagc
     4141 taaaggaccg gtttctgctt caccatcacc aatcacgaca gcagagatta cttcaggatt
     4201 gtctaataca gctccgactg catgtgaaag agagtatcca agctctccgc cttcatggat
     4261 agaacctggt gtttctggtg ctgcatgaga agcaatgcct ccaggaaatg aaaatcgttt
     4321 gaataatttt tgcattcctg tctcatcttc cgttacttct ggatagatct ctgtgtaact
     4381 tccatctaaa taagcattcg ataccatgac ttgtccgccg tgtcctggcc cttcaatata
     4441 aaacatgttc aaatcatatt tattgatgac tcggttcaaa tggacataaa taaaattttg
     4501 gccaggaatc gttccccaat gaccgattgg atgttttttg acatcttcgg gtttcagtgt
     4561 acgtctcagt aaggggttat ctttaagata aagttgccca acagaaagat aattagcagc
     4621 acgccaccaa gccgtcattt tatcaaagta ttctttacta gaataatcca tacataacac
     4681 tccttttcat tattactctt attctagcgc agatactccc aattgaaacc taataagcac
     4741 ttctgttcat ttgtttggat tttatatcta gccaaatgaa caggtataaa aaaccacgaa
     4801 atttttcctt tcgctgacgt tttttacatt gatccaaaaa acttgtcgtt atcacaatgg
     4861 ctgttccgca gttcatttcg ctcctcatca tgcataattt gctgaatgac gctgggccag
     4921 ttaacacatt gctggaaaac tggggctgga tcgagcaatt cctttcctga ttgatccgtt
     4981 ttatgtaaaa gtctctgtta tctttgtcaa tatgtgaatt ggtatcccag ctactatgct
     5041 aatttctaca gggattatcc aaaatttgga tcgtgaccaa atcgaagcgg cgtaatcgga
     5101 aatatcaata acttcaacgt gatttttctt ttaactgtca acggaccgac aaactcaaac
     5161 ttttatggtg caggttccac tgatcttcta gttacttggc tatacctgac tgtcactaaa
     5221 atagattaca atctagcttc cgttatcggt atcttgatct ttatctatat gcaaaaattt
     5281 atatactact agaaaaaaga aagtcgaatt ttttactttt ctttttctct tttttggtta
     5341 gctgacacgc cgcaagacta aagtcatgga actcttaggt aatacgtctt aatgaaaaca
     5401 aaaaaatccc gcaacccctg atttcacagg atttgcggga gtttttgatt agtctttgtt
     5461 caagtcgcga agcaattcat cgatatggtt gccatagaca actgattcat caagttcaaa
     5521 gaacaattca ggtgtcttat aaatacttag attgtgccct aactctcgac gaatcaatcc
     5581 tgatgcttta ttcaatccat cttgtgctgt tttcttatct gatgctaagt cagataaaat
     5641 gctgtaatag atcgttgctt gctgtaagtc ccccgtaact ttcacatctg tgatcgttac
     5701 gttctccaca cgagggtcac ggaccttttt gcgcaaaata tcattgactt cttttaagat
     5761 ttcttgaccg actcggcggt cacggtagtt agccataatg attggcctcc ttttatcagt
     5821 ttttagtcat ttttgacttc ttccatgata aatccttcaa ttacgtcatc tactttgatg
     5881 tcattgaatt tttcaatcat tgcaccacat tcataaccca ttttgacttc tttcacgtca
     5941 tctttgaatc gtttcagact tgcaagttgg ccttcataga ttacgattcc gtcacggatt
     6001 acacggacac cgctatctct acgaatatat ccttctgtta cataggcacc tgcaatcgta
     6061 ccaactttag aaactttgaa ggtttcacga acgatcattt gaccggtgat tttttcttca
     6121 aattcagggt ctagcatccc tttcatcgca gtttcgattt cttcgatggc tttatagatg
     6181 atacggtgaa gacgaatatc gacttcttca gtatcagctt gcacttttgc ctgcggtgta
     6241 ggacgaacgt tgaaaccgat gataatagcg ttgctggctg ctgctaaggt tacatcactt
     6301 tcattgattg ctccgacagc tgaatggacg atttttacac ggacaccttc cacatcaatc
     6361 ttcttcaatg atgcagccag tgcttctgct gaaccttgta catctgcttt gatgattacg
     6421 ttaacttctt tcaattcgcc ttctttcagg ctttcaaata agttatccaa agtcacacgg
     6481 cttgtggcag cacggttttc taataatgca cgtttcgcgc gttcttcacc cgctgcacgt
     6541 gctgtctttt cgtcttcgaa gactacgaaa cggtcacctg cttgaggtac atcattcaag
     6601 ccagtgattt ctactggcgt tgcaggtcct gcttctttat cgcgacggcc aagatcgttt
     6661 gtcattacac ggacacgtcc gtaagtatta ccgacaacga ttgggtcacc aacatgaagt
     6721 gttccttctt ggacaagtaa ggttgacaca ggacctttac ctttatcaag acgtgcttcg
     6781 atcactgttc cgattgcgcg ttgtgttgga tctgctttta aatcttctac ttctgcgact
     6841 aacaggatca tttccagcaa ttcttcgatg ttttggccga atttcgctga gatctcaaca
     6901 aagatcgttt ctccacccca tgcttcagga attaactcat actcacttaa ttcttgcata
     6961 acatgttgag ggtttgcacc tggtttatca attttattta cagcgacgat gattggaacg
     7021 cctgcagctt tggcatggtt gattgcttca actgtttgtg gcatgacgcc atcatctgca
     7081 gcaacaacta agatcgtgat atctgtgata cttgctccac gtgcacgcat acttgtaaag
     7141 gccgcatgtc ctggtgtatc caagaaagtg attggtttac catcaatatc gatctggtag
     7201 gcaccgatat gctgtgtgat tccgccggct tcgccgcttg ttacacgtga atggcgtaat
     7261 gtatccaata aagttgtttt cccatggtct acgtgtccca taatcgttac gactggcgga
     7321 cgtgagacta atttatctgg atcgacttct tctgcctcga agaatttatc gatatcagca
     7381 atgtcaactt ggactttttc ttgtggttcc atgccatagt ccgtcgctaa cagttcaatc
     7441 gtatctttat ccagtgcttg gttttgattg accatgacac caagcataaa taatttttta
     7501 atgatttctg ctggctcacg atgaattttt ttcgcaatat ctgctacgtt cataccttca
     7561 gtatactcta atacatctgg caattcacgg aatttgcgtg gtggtacagc tggtttagtt
     7621 gattgttgct gtttgccttt tttacctttt ttattataac gattacggtt attattatta
     7681 aatttgccgc gattgttatt gtttggtttg ttgttattgt tagtacggtt ttgattgccg
     7741 ccttggctcg ttccttgacg attttggctg ctggtatttc ccgtattatt ttggtttgtt
     7801 gttggttttt ttccttgatt cacttgaccg ctcccttgac cgcgatcttg ataactgcgg
     7861 ttatttttat ttttttgttg attgttcgtt gtttcttttt tacgttgatc gttcggctgt
     7921 tgattggctg gttttctgct tgcttgttgt gcagcttgtt gtttcgtgct tgggtttttg
     7981 aacgcttgtt gcaatttctt ctcatcattg ctagtaatcg cacccatatg gtttttcaca
     8041 tcgatgccaa gttgttgtgc tttttccacg acgtctttac tagatttttt gagttctttt
     8101 gctaattcat agattctttt gttgcccatg caatcacctt cctaaccttc aattagctcc
     8161 ttggtcttct tcgcaaatcc ggcatccatt attccgaata ccttgcgagg ttttccgatc
     8221 atctgcgtga tttctgcttc agaaaatagc tcaaagcaag gaacttcata atatgaactc
     8281 ttatctttta tcttttttct tgtattctcg cttgcatcgg ttgccacaaa caccaatttt
     8341 gctttttgtt tgcggatttc agcaatggtc agttcttcac ccgtgattag ttttcccgcg
     8401 cgcatcgcta gaccgatgag attcatggct ttttgccgat taactgtatt cattttccaa
     8461 ataactcttt acgtgctttt tggtgagtga catagtcaag aagttcttga taaaactcat
     8521 ctgttaatgt tgtttcgagt actcgatcga gaattttttt atcccaagct tgttgtactt
     8581 cttccggctc aagtgagaca tacgctcctc gtccaggcat ttttccagtt ggatcgatcg
     8641 atacttcccc ttcttttgaa cgggtgattc ggaccatctc ttttttaggt ttcatttcac
     8701 ctgaaaccac ggatttgcgt aaaggaattt tcctttgttt catctctttg cctccttact
     8761 aaagttaagc ttctggtttt tcttcgaccg tttcattgtt gaatgcaatt gtttcatatt
     8821 catcgtctgt taaatctgac tgaacgattg cttcatcatg cagttcttct gtttcttttt
     8881 gttgttcttg attttcataa aattccgtca tgtcagattc tgatttgata tcgattttat
     8941 ggttagtcaa tttagcagct aaacgtgcgt tttgtccgcg tttaccgatt gctagcgata
     9001 attggtagtc tggaacgaca actgtgcagg cctttggatt tgtctcatcg aagatcacat
     9061 ccatgacttg tgcaggattc aatgcatttg caatgaatac agctggatct tcattccatt
     9121 cgacgatatc catgttttcg ccttttaatt cattgacgat tgcttgaact cgttgtccct
     9181 ttggcccgac acatgtaccg actgcatcga tatttggatc atttgagcga actgccactt
     9241 ttgaacgatc gccagcttca cgggcgatgc tgacaatttc aacgattcca tcatagactt
     9301 ctggtacttc ttgctcaaat aagcggcgca ataggtctgg atggctgcgg ctaacaaata
     9361 cttgcggacc tttagacgta ttttctactc gggaaacata aactttgatt cgatcatgtg
     9421 gttgataaaa ttcatttggc atttgatctt gtttagaaag aactgcctca attttaccaa
     9481 gattgacata aatataacgt ttatcctggc gttcgacgat tccttgcatg atgtcttttt
     9541 cataagcact gaattcgtta taaataatcg ttctttctgc ttcacgtaca cgctgtaaaa
     9601 taacttgttt agccgtttgc gcagcgatcc gcccaaaatc ttttggtgtt acttcaaaac
     9661 ggatcgtatc accgatctca tatgctggat tgattaaaag tgcatctttc aatgagactt
     9721 ccaactgtga atccatcact tcttccgtta cttcttttac cgcatagaca tggatctttc
     9781 cttttttctg atcaaattcc acttcgacgt tttgtgcttg gccatagtga cgtttgtatg
     9841 cagaaaccaa tgctgcttct aaagcatcga tcacgatttc ttttgagatc cctttttcag
     9901 cttctaaagc gtctaacgca tttaacattt ctttactcat tttttctgat tcctctccgt
     9961 gattaaaatt ggatagctaa gcgagctttt gcaatatttt ttcggtcaaa agtcaattct
    10021 ttttctcgtg tttttatgcg tattttcaat gtaagctgtt cagcatcaaa tgattgaaga
    10081 aatccttcat attgtttttc gccatctact ggttggtata aggagacatg gacataatca
    10141 ccaagtgcct tttcatagtc gctttctttt ttcaatggcc gctctgctcc cggggaggat
    10201 acctccagga aataagcttg aggaatcgga tctggatctg ctgcatcaag tttttcactc
    10261 agtttctcac tgacaaaggc gcattcttcg atatcgatac cgccttcttt gtcaataaat
    10321 actcgtaaga accagctttt tccttctttt acaaattcga cttcgacaag ttcaaaattt
    10381 tgttcatcta aaatcggtgt caccatttct gtgactgttt caacgacgct actcaaaagt
    10441 ttcgcctcct taaaaaatta agtgcgtaat tccattaaaa agagtgagcg aaaaatccgc
    10501 tcactcacag ttagtatcat tacctataag aactatacca tactctgctt aaaattacaa
    10561 gcagacagga aaacagatgc ttatagcata tcgaataacg ataattgatt ttcatctggt
    10621 agatctttca atacgccatt gtcgttcata tattcgatca atgtttttga aactttaccg
    10681 cgggtggcaa gatcttcttt ggataaaaat ggaccgtctt ttcttgcttc aacgatttgt
    10741 tttgccacgt tggttcccaa acttgggaca gctctaaatg gtgcgatcaa cgtatcacct
    10801 tcaataacaa agttaacggc atccgattta tacaaatcaa tcattccaaa tttgaatcct
    10861 cgttccagca tttcattggc taactccagc actgtcagtt gattcttttc ttttacggaa
    10921 gcatctaatc ctttgtccgt gatttctttc attgcttgtt tcacagcatc ttttcctttg
    10981 cacatggaga ctagatcaaa gtcgtctgca cgtacagaaa agtatgcaca gtaatacaaa
    11041 atagggaaat aaaccttgaa ataagcgaca cgaagagcca tcaatacgta agctgcagca
    11101 tgggctttcg gaaacatata tttgattttt gaacaagaat cgatatacca gtcgggcaca
    11161 ttattttctt tcatcgccga tagataggtt tctcgcaatt catcagggat cttgttccat
    11221 tgtcctttac gaacagtttc catgatttta aatgccatcc cgctatctaa tccagcatgg
    11281 atcaaataaa ccataatatc atcacgacag ccgattactt ccgccagtgt cgcatctcct
    11341 cgtcgaatca attcttccgc atttcctaac catacgtctg taccatgaga tagtccggaa
    11401 atttgcagta gttctgcaaa agttgtagga tgcgtttctt ctagcatccc tcgaacaaaa
    11461 cgagtgccga attctggaat acctaatgtc cctgttttgg aataaatttg ttcctgactt
    11521 actccaagta cttcaggacc ggcaaatatc cgcatcactt ccgggtcatc cgtagggatc
    11581 gtttgtggat cgatccctga taaatcttga agcatccgaa taacagttgg gtcatcatgc
    11641 cctagaatat ctaatttcaa tacattatcg tgaatcgagt gaaaatcaaa atgagtcgtt
    11701 ttccattccg aattttgatc gtctgctgga tattggatcg gcgtaaaatc atagacatcc
    11761 atataatcgg gaataacgat gatcccccct ggatgctggc cggttgtccg tttgactcca
    11821 gtagcgcctt ttgctaatct gtcgacttct gcacttcgaa actgcagatt attgtccctt
    11881 tcgtatcctt ttacatagcc atatgctgtt ttgtcggcga ctgtaccaat cgttccggca
    11941 cggtaaacat agtcttcgcc aaacaatact ttcgtatagt tatgggcttc tgcttgataa
    12001 tcacctgaga agttcaaatc aatatcgggc actttgtctc catggaatcc taaaaatgtc
    12061 tcaaatggaa tatcatgacc gtctttgttt aagcgtgcac cacattttgg acattgtttt
    12121 tctggcatgt caaatccaga accatacgta ccatcttcaa aaaactcgga atattggcac
    12181 tctggacaat aataatgagg ggctaacgga ttaacttcag tgattcctgt catcgtcgca
    12241 acaaagctag agccaacaga gccacgtgaa cctactagat agccatcttc attactttta
    12301 tgcactagtt tttgggcgat cagataaata accgagaagc cattaccatt aatagaattc
    12361 aactcttttt ttaatcgttt ttgaatgatc tcaggtaacg gatctccgta catttgtttt
    12421 gcttttgtgt aactcaattc tgatatttca tcttctgaac ctggaatctt cggtgtataa
    12481 agttcatctt tgactggaat cacttcttcg cagatatctg cgattttatt cgtattttct
    12541 actacgatct ctttcgctgt ttcttcccct aaaaagtgaa atgcagttag catctcatcg
    12601 gttgttcgga aatggacatc cggcaaacta tgacgattca acggattcgc tccgcccata
    12661 gaattgatca aaatttttcg gtaaatggca tcttcttcgt tcagataatg tacattacct
    12721 gttgcaacaa cgattttatc caaacttttt ccgatttcta ccaaattttg gataatttct
    12781 tccaaatcat gttcattttt taccaattct tgctcgatca aaggcgcata gaccgctttt
    12841 ggcataactt caatataatc gtagaatttt gctcggtttc ttgcctcttc tacccctttt
    12901 tgcatcatgg cttcgaatat ttctccctta tcacaagctg agccgatcaa tagattttca
    12961 cgcatctttt tcaattggga gcgtggtatt cgtgggacgc gttcgaagta ttcgacattc
    13021 gacattgaaa tcagtttgaa caaatctttc agtcctgcct gattttttgc taaaatcgtt
    13081 acatggaaag gtcgagccct tttgtaagaa tctccttctc cgatatgctc atttagctga
    13141 tcatgataaa gcatgttatg attgtccatt gcctctttca caaagatcca tgctaagtga
    13201 ccagttgctt cagcatcata gattgctcgg tgatgctgtt ccaagctgac accaaatttt
    13261 ttagataaca cacctaatcc gaaacgtttg aattgaggat acagatatct agctaattcc
    13321 aacgtatcaa tgactggatt ggccgcttca ggaataccat accttgcgta gctagtattc
    13381 aagaacccca tatcaaaagc cgcgttgtgg gctaccaaaa ttgtatcttt tgaaaattca
    13441 aggaacatcc gcaatacttc ttcttccgac tttgatcctc gaaccattcc atccgtgata
    13501 ccagtcagat ctaccgttgt tcgtgacaaa ggatgtcctg gatcgataaa ttcatcaaag
    13561 ctttcgatca catttccttt atacattttg actgcggcta gctcgatgat cgtgtcatat
    13621 actgcagaca atcctgtcgt ttccacgtca aatacgacat atgtggcttc actcaatgct
    13681 tcatgtgcat cattataagc aattgggaca ccatcatcta cgacattcgc ttccacgccg
    13741 tataagattt tcacacctgc tttttttcca gcactatgtg cttccggaaa ggcttgcgca
    13801 ccaccgtggt ctgtaatggc gatagctcgg tgtccccatt tccctgcttg tgccacaaga
    13861 tcagagatgc tgtttgttgc atccatcgta ctcatattgc tatgaacatg aagctctact
    13921 cgtttttccc cttcaggagc ataatctttg cgaggtgtat gtttgacttc tatgatatct
    13981 tgcgcattca tgaccaaatc acggacaaac gtatcttctt gaatacttcc tcgtaccttt
    14041 aaccagctac cgacactgat cgcatcaaat acttgttcat ctttttcgcc gttcgagaat
    14101 tttttgacga taaatgatga agtataatct gtgattttca aagtcaagat cttgcgtttt
    14161 gaacgtagtt ctctgacttc tttgtcgaat acgtacccct caagtgtgac tcttcgttct
    14221 tcttcaacga tactgatcat tggtgtcgtt ggttcatcgg ctggaatatt tcgtcctagc
    14281 tggatgtgga tgggtccttc aagagctggc gattgctctt ttctttcctt tttcttttgt
    14341 tcgtggacaa ctagactttc agctgcttgc ttcataaagg cttctgcctg ttcttgtttg
    14401 cgttcttcaa aaagtttcaa tactcgttcc gcttgctgct catccacttc tggttcgata
    14461 cgaaattttg ggaatccgta actgacgaac aactcttcta ctaatggcaa gtattgttgc
    14521 ttcaaataag aaatagctcc tgtactgtcg attggcaaga tgactttacg atcttttatc
    14581 acaggtacct gcgttttcaa tacttgctgt gctagtggtg tatcgcattg ctgattttcc
    14641 aaagcttggg cccagtaatc ttgcaagagc tgttcatcga atgtctggtc atctgctaag
    14701 atattcagtt tgacttgtgc aatatcttta aaagcgatag tcagatgctg agtcaatgac
    14761 cgatatagca taataggcaa aattttggaa aaatgcagcg taaattccca taaacgcgat
    14821 ttacgatgga ccactacact atccatttca ccagcagtaa gcagtggatg atttttttct
    14881 gcttcctcta gttggatttg ttccaacagt ttttcaaata aatctcgttt ttctgacaaa
    14941 gatatacgcc ctttctgatg agaaacaatg caattgatcg catcttatcc tcttcttttt
    15001 cagtaccttt tagtatactc gttttttctg agttttacat taaaaaattg gaactgcgat
    15061 tcaaatcgca gttccaattt taaaagcgaa tcatcagatt gtcaaaaaca gctgattcag
    15121 ctatttttat tttctgtgtt ttaacgctta ttattcgcct tggttcaaga ggattggtaa
    15181 agtattcacc aattcttctt tacgtacttc aaccatttcg ccagttttct tgattttcac
    15241 ttctacgatt ccatccactg cttttttacc gacagtgatt ctgattggac agccaatcaa
    15301 atcagaatcc gcgaatttca ctcctgcgcg ttcatttctg tcatcaacca gtacttcata
    15361 gccagctttt gtcatcgctt cttctagttc attcgtcagg tttgtttggt attcgtcttt
    15421 tagattcatt tggacgatat gcaaatcaaa tggcgcaatt ccttttggcc agttgattcc
    15481 ttgttcatct gaatgttgtt ctacgattgc tgataataaa cgacttacac cgataccata
    15541 acagcccata atcacaaatt tttctcgtcc attttcgtct agtacagttg ctcccataga
    15601 ttcactgtaa cgagtgccga ttttgaagat atgtccgatt tcgattcctc gagtaaaggc
    15661 aagaacacca ttgttgtctg gtgaaggatc tccttcttgt acaaaacgaa gatcttcata
    15721 agaaagtaca gtgaaatcgc gttctggatt cacattttta tagtggaatc ctgtttcgtt
    15781 cgctccagta atggcattcg ctaaatcttg gacataacgg tctgcatata cttttacgtc
    15841 atctgaaaca ccgactggtc cgacagaacc aaactcagca cctagatatt tttgtgcttc
    15901 ttcttctgtt gcttcttcta aaaagtcagc agataaaaag tttttcagtt ttacgtcatt
    15961 gacttcatgg tctccgcgta ccaaaacaag gacaggttgt tcatccgcaa tgaacaatac
    16021 agacttgatg attttttgag gttcaacaga aaagaaatct gctacttcgt caattgattt
    16081 tacgttaggt gtagctactt tctccaattc aagctgtgtt tcatgcgatt tttttgatac
    16141 atagtagctt gtagccatct ccaagtttgc agcataatca ctttcagtcg aataacagat
    16201 cgtatcctcc ccgatttctg agatcgccat gaattctttt gagtctttcc cgcccattgc
    16261 gccaccgtct ccgataatcg agcgaaattc taagccacaa cgcttgaata cttcggtgta
    16321 ggctttttcg taatctttat atgtctcgtc caaactttct tccgttgcat ggaaagaata
    16381 cgcatctttc atgataaact ctcttccgcg aagcaaaccg aagcgaggtc gtttttcgtc
    16441 acgatattta ggttggattt gataaagatt taaaggtaat ttcttatatg aattgatttc
    16501 attacgcatc aaatcagtaa aagtttcttc gtgagttggt ccaaggatca tttttcgatc
    16561 attgcggtct ttgaaacgat acaagtttgg tccatatgtt tcgtaacgcc cggattcttc
    16621 ccaaagttct gctggcaaga tggcaggcat cagcatttct accgctccga ttttttcaaa
    16681 ttcttcccgc ataatgcgtt tcagtttttc caatacacga tttgctaatg gcagatatga
    16741 ataaataccc gctgatactt ggcggatata tccagctctt agcaagattt gatggcttaa
    16801 tacttctgca tcatttggaa cttcgcgtaa tgttggaatc aacatttttg attgtctcat
    16861 tctatatact cctttatttc ctcaaaatag ttatcgtttg ttattatacc ctatttgtca
    16921 actttttcta gaaaaagaat cgttgaatat cgttccatgt aactagcacc atcaacaaca
    16981 ttaaaaatcc aaagccagct aatgtcaata ttccttcttt ttcttgactt aatggtttcc
    17041 cgcgaatccc ttcaaaaata ttcaagacta atttcccgcc atctaaggca ggaatcggaa
    17101 gcagattgac gattccgaga ttcattgaaa gaagcgccat caaactaata acagttgtta
    17161 caccttgatt tgctgcttct gaagacaatt ggtacatcat gacaggtccg cctaatttat
    17221 ctaaactgaa acctgtaaat agtgatccta aggctttgaa aatttccaat gaaccgctga
    17281 aagcttgacg cgtccctcca atgatcttat ccataaaacc tgtattcatt ggggctttta
    17341 taccaagttg gccaactttt tcgccatttg actctacaga tttcggtgtc acgtcgacag
    17401 attgcacctg accttctcgt tcgatcttaa agtctaatgt tttatccgga tttttcgtaa
    17461 tgactgttgt cagatcattc catgaatgga tttcttttcc gtccacactg actacctcat
    17521 cattttcttt taagcctgct tctgccgcag ctccattagg catgatctct cctacgcgat
    17581 tagtgttcgt cacttgaaca ccaccttgca taaatgccag aatgatgaat aacaggattg
    17641 ccaaaataaa gttattcatg ggtccagcaa agttggtcag catacgctgc cacaatttag
    17701 ccgattggaa ctgtacgtct tttggcgcaa ttctgatttc tgttccgtcg ttttcaataa
    17761 tcgtcgcatc gtgatcaaca gggtaggtta ctacttctgt ttcgtctcca ttcacataac
    17821 cagtgatagt cagctcatct tctaaatcat aacggctcaa ttccatcggg atagcatttg
    17881 tcaattggat tttcttactt aaatttatct tctctacgat gccgtcagaa tttagtaaaa
    17941 gagaaagagg cattcctggt gccatttctg tttcatcatc cccattgcct gccattcgaa
    18001 cataaccgcc aataggtaat aggcgaagcg tatatgttgt cccgtctttt gcttgatgcc
    18061 cataaatttt cggacccata ccgatagcaa attcgcgaac gaggattcct gatcgtttcg
    18121 caaagaagaa atgaccaaac tcatgaacaa tcactaatat accaaaaacg atgataaatg
    18181 tcagaatcgt tttcatagct tttttctcct ttacttgttt tttgtgtttt ttataaaaca
    18241 acgataaaac actataattt taccatagta taccaaagac accaatagta tcaatataat
    18301 gaaaaataaa aacctgcgtc ttttatgaaa ttcacaaaga aacgcaggta tacttactaa
    18361 ataatgccaa ataaatgcat gataggaaat acaaacagca aactgtcgaa acggtctaag
    18421 attccaccat ggcctggtaa gatatttccc gagtctttca catcataatg acgtttgatt
    18481 gcagattcga ccaaatcacc aaattgtccg actgctgaaa aaataatcgt catcagaagc
    18541 atgacaaaca taccatgatt aaatagttct tttgctgggt atagcaataa ataaagaaat
    18601 gcgactacaa cagcactacc aataccaccg agcgcacctt caatcgtttt gttgggcgaa
    18661 atatcaggac ttaacttatg ctgaccataa cgtcgaccaa ccatatatgc tccgatatca
    18721 gttgcccaaa caataaatag accaaatatc aaaacagcca agctttctgt tcgtgcattg
    18781 acaaaattct ggaaacctat tcccacatac aagctgacaa gcacaggaaa acctgcttca
    18841 tcaatcgtat acgtattttt agaaataact gatgttccta aaagaatcat aactataaga
    18901 taaaataaca taaatgtact tacattctct ggaagaaaga aaaaccatct ttcttttggc
    18961 aaaacaagta acactgcacc aacggcggat aaaactcctt caaaacttaa gagtgtcaat
    19021 cctttcatcc gaaacagctc gtaaactcct attactgcta agattgctgc tgtaagctca
    19081 accgctatgc ctccgatcca tataatcgga ataaacaaga ctagcgccac ggctgctgtg
    19141 accacacgtt gtttcatgac tgatcccctt tctcatcttc tgtttttttt acaccgccga
    19201 atcgacggtc tcgattttga taagaggcga ttgcttcttc aagatgcgca ccatcaaaat
    19261 caggccaaag cgcttttgta aaatacagct cactatatgc gatctgccat aacagaaaat
    19321 tactaattcg ctcttctcca cttgtacgga tcatcagctc aggatcttgt aactctttag
    19381 gcaagaaacc tgtcattaaa tgcttagcaa tcaattcatc atcgatttct tctgcgtgta
    19441 tttctccttt tgctacttct tcggcaattt cttggacagc agtcacgatc tctgctctgc
    19501 ttccataatt caaagcaaaa ttcaatacca ttccagtatt gttttttgtt tgctcaattg
    19561 cacgtcttac agcatcttgc gtatgttctg gcaatacgtt ttcataaccc atgacatgaa
    19621 ctttgacatt ttctttaatc aattctggta cgaacgtatc aaagaagtcg acaggtagtt
    19681 gcatgagaaa acttacttca tcttttggtc gcttccagtt ttcagtcgaa aaggcataaa
    19741 gcgtcaacac tttcactcca agccttgatg ctttttttgt gactttcttt actgtttcca
    19801 tgccttcttt atggccagca actcgtggca aacgacggtt ttgcgcccaa cgaccattgc
    19861 cgtccatgat gatggcaatg tgctgaggaa ttggtccatc ggtattaaat tgaaattcgc
    19921 ttttttcttg aatatatttg tttttttgcg gaaaaaaacg taacattctt tttcctccta
    19981 tacttaaaag attcccacta tcttaagtat tatagcaata tctaactgtt tttcctatga
    20041 aatcattgtt tttttattta aaaactgaat aattttttct ttttttaatt tttaaataaa
    20101 agaaatataa ataaaaacag aggaagcgga atgatccgcc cctctgtttt tatgatttta
    20161 ttaaacttcc aataattctt tttctttatc agcagcgatc ttatctactt ctttgatgct
    20221 gttatctgtc aatgtttgta cgtctttttc taatccgcgc aaatcatctt cggtaatatc
    20281 accatttttt tgttgttttt tatattcatc gatcgcatca cgacggatgt tacgaatcgc
    20341 gatttttgca ttttctgctt cttttttaac atctttagct aattcttttc tgcgttcttc
    20401 tgtcagttgc gggataacta agcgaatgac atttccatca ttggttggac tgattccgat
    20461 atcacttgcc ataatagctt tttcaacatc ttgaagaatg ctcttgtcaa aaggtgtgat
    20521 cattaaaaca cgtgcttctg gaatttggat tgatgccatt tggttcaatg gtgttggcgc
    20581 accgtagtag ttcacagtaa tacggtccaa aaggctagcg ttcgcacggc ctgcgcggat
    20641 ttgcccaagt tcacgttgaa gattttgtgc tgcttttttc attttttctt ttgcttctgt
    20701 cataattgca tctgccattt ttatttcccc cttacagttg ttccgatatt ttcgcctaag
    20761 attgcacggc gaatatttcc gtgttcattt aaattgaaga caagcagtgg aatatcatta
    20821 tccatgctta gtgaacttgc agttgaatcc atgacttgta atcctttggc aataacttcc
    20881 agatgagtca gttcttcaaa ttttactgca tttgcatcta ctttcggatc agcagaataa
    20941 acaccgtcta cattgttttt agccatcagg ataacatctg cgccgatttc tgccgcgcgt
    21001 aatgctgctg ttgtgtctgt cgagaagtac gggttgccgg taccgcctgc aaagataacg
    21061 atccgtcctt tttctaaatg acgttctgct ttacggcgaa tatatggctc tgcgatttga
    21121 cgcatttcaa tcgaagtttg cacgcgtgtc ggtacaccca cgttttccaa cgtatcctgt
    21181 aaagctaagg cattcataac tgtagctaac atacccatgt aatcagcttg agcacgttcc
    21241 atgcccattt gtgctccgat ttgtccgcgc cagatatttc cgccgcccac aacgattgcc
    21301 atttcaacgc ccagctcgtg aacttctttg atttcttgga tgatttcttt gattactggt
    21361 ggtttgattc caaatcctgc gtccccagct aaagcttctc cactcaattt taagactaca
    21421 cgctgatatt tcggtgtgac catgttcatt cctccaatat tttctattgc cattttacca
    21481 tacttagatg atttcgacac tctaagactg gtttttcttg ttttgcttaa aaaaagggag
    21541 cgcacaaacc gtgcgttccc gtctgttatt caacagaatt attttttcac ttggctcata
    21601 acttcttcta caaagttatc ttcgcgtttt tcgatacctt cgccaacttc gaaacgtacg
    21661 aatgttttaa cagttgcgcc ttttgatgca acgtattttt caacagtcat atcaggatct
    21721 ttaacgaatg gttggtccac caaagcgatt tctgctttga atttcttcaa gcgtccttct
    21781 accatttttt caacgatatt cgctggtttg ccttcgttca atgcttgttc tgtaagaaca
    21841 gttttttcat gttctaattc agcttctgga atttgtgatt cgtttacata acgaggattg
    21901 atagctgcta cgtgcatagc tacgtctctt gcaactgttt cgtcagttgt tccatctaga
    21961 acagtcaata cggcaatacg tccgcccata tgcaagtatc caccaaatgc agcattgtcg
    22021 tctttttcta caacttcaaa gcgacggaag ctgatttttt ctccgataac ttgagttgct
    22081 tcgatcaagt cagattcaat tgtaccttta tctgttttga ttttcattgc agcttccatg
    22141 tctgctggtt tgttttcagc aacaagttct gcaatttctt ttactaaatc ttggaacatt
    22201 tcgtttttag aaacaaagtc tgtttctgag tttacttcaa cgattgctgc agtgttacct
    22261 ttcacagcaa ctgaagccaa tccttcagct gcaatgcggt catttttctt cgcagccttt
    22321 gccattcctt tttcacgtaa aagatcaact gctttttcca tgtcgccttc tacttcaact
    22381 aatgcttttt tcgcatccat cattcctacg ccagtcattt cgcgtagttc ttttaccatt
    22441 ttagctgtaa cgtctgccat gtttaactcc tcctgtgtgt ttttcttttt aaaaaaagct
    22501 gcttcaaagg agagtgaggc ttgtcgccca gcctttgaaa cagctccatc attcaaaatt
    22561 attctgctga agaattgtcg ccttcaacaa catctacgat ttcttcgatt gatgttgctg
    22621 tgttttcttc tgtgaaatct tcttcaacta cttgatcttc accttggttt ccttcgatga
    22681 aagcatcagc catttttgaa gtaatcaatt taacggcacg aattgcatcg tcgtttgaag
    22741 ggattactac gtcgatttca tctggatcgc agtttgtatc aaccatagct acgattggga
    22801 tatttaattt gtgtgcttct tgaacagcaa tacgttcttt acgtgggtca acgatgtaca
    22861 ttacatctgg gattcttggc atatcagcga taccgcctaa gaatttttca agacgttcac
    22921 gttctttgtt caaaccagca acttcttttt taggtagaac ttcaaaagtt ccatcttctt
    22981 ccattgcatt gatttgtttc aaacggctga tacgtttttg gatagtatcc cagtttgtca
    23041 atgttccgcc taaccaacga tggttaacga agtattgtcc tgcacgagtt gcttcttctt
    23101 tgattgcttc ttgtgcttgt ttttttgtac ctacgaataa tgctacgccg ccttcttcag
    23161 caacgctctt catgtaatca taagctgcat ctactaattt aactgttttt tgcaagtcaa
    23221 tgatgtagat tccgtttctt tctgtgaaga tatatttctt catttttgga ttccagcgac
    23281 gagtttggtg accaaagtgt acgccggctt ctagtaattg tttcatagaa attactgcca
    23341 tgtttgtttc ctccgatttg gttttatttt tcctcttctt atcttctacc ttgccggcaa
    23401 actttttcaa gcacctaccg acaatccaat aagaatgtgg atttgctgat gcataaagca
    23461 ccctcagcta ttatacagac aaaagatccg tatcgcaaga gcaaatatac ttttctgctc
    23521 aacattcaat agtttacctg aaagataaaa agaattcaag aaaaacagta gatttctctt
    23581 tgtttcttta ttatcctttt tccataaaat aattttaggt ataaaagacg aatattattg
    23641 ggcaatcctt cttttttttg ttatgataca tgtgaaatct ttcgtaaagg agaaagaaaa
    23701 tgcgcaaagc tcaaagacat ttgctgatta aacaagtcat tgaagaattc ccgatccgta
    23761 cgcaagaaga actattaaac aaacttgggc attatggcgt taccgccacg caggcaacga
    23821 tttctcgaga tatccgtgat ctaaaaatcg tgaaggcacc tgatgaaaat ggcgtttccc
    23881 gcttcgtatt gtttcgcgaa aaaggaactc ctgagactag gactgaggaa gaggatcggc
    23941 tggttcaaat gattgaagac attgtattga aagtcgaacg agtccacttt ttgacgatcg
    24001 tcaatacctt gccagacaat gctcatttat ttgcatctgt tttagatgag atcaaacctc
    24061 cctctattgt cagtacaatt gctggattcg atacgatcat ggtcatctcg gaaaatgagg
    24121 aggacgcaaa acgagtagaa gaatttcttc accatccaac tgaagtatca cttttttaat
    24181 cagagcacgt attcctaata tggaatgcgt gtttttttat tttcaatatt ttccttattg
    24241 aataaacctt tacactcttt acaaaaatac atcatttttt attcaaattt ttcaacacct
    24301 tgattgaatc acaataatca tcatttatat tggatgttaa caagagcagc ttcttgctct
    24361 ttgtgtattt caaaaaagaa atgaaaggaa atcatatgta taaattatta acttctggac
    24421 cattgacgac ttctgaaaca gtgaaaaatg ctatgctgat cgatcattgt acttgggatc
    24481 aagaatacaa agaaattaca caatggataa gaaaaaaatt attggatctt gctcaagtat
    24541 cggaaggaat atacacaacg gtcttaatgc aaggtagcag ctccttcggg gtagaatcgg
    24601 tattaagttc tgtgatcagg ccagaagata ctgtactgat ttgttcaaac ggcgcatata
    24661 gacaacgaat gatcaacatg tgcgaatgtc tgaaactgaa ctataccgtt tatgaggtat
    24721 cggaacatca aatcctccaa gctcatgtga ttacacagct gattgaagag aatccgtcta
    24781 tctccgcact agcaatggtc catagcgaga ctacttccgg tattttgaat aatttggaag
    24841 agattgctgc tgtcacacaa gaaagagatc ttttgtttat agtggacgcg atgtcaagct
    24901 ttggtgggat accgatttgt gtagccgaac tagggattga ttttttgatt tccagctcaa
    24961 acaaatgtat tcaaggtgtt ccaggctttt cttttgtact tgctaataaa gcggaactgg
    25021 aaaaacgaaa aaatgttagc cgaagtttgt cacttgatct atatgaccag taccgcgtga
    25081 tggaaaacga gaatggcaaa tggcgtttca cttcccctac ccatacggtt ttggcttttc
    25141 aacaagcgct gaaggaacta gaggcagaag gaggaatcga aactcgatat tcacgttatc
    25201 tcgccaataa tcatgtgcta cgacaaaaaa tgacagagat ggggttccag cctttcattc
    25261 aagaatatca ggggccattc atcacctctt ttctttaccc taaggactgg cgatttgatt
    25321 ttaatcattt ttatcagtat ttaaagcaaa atggatttgt gatctatccc gggaaaatca
    25381 gcgatcatga atgttttcgt atcggaaata ttggcgagat ctatccggaa gatattttgg
    25441 aactgacagc aattatcaaa gactataagg agaacgttaa tgattaaaac tgtaattttt
    25501 gattgggcag gaacaactgt ggactttggc tgtatggctc ctgtccacgc ttttagaaat
    25561 gcttttctag aaaaaggaat ccaactgact gacaaagaaa tccgctaacc gatgggaaaa
    25621 ttaaaatggg atcatatcca acagcttctt tccctccctt ctgtcaaaaa acaatggata
    25681 gctatttatg gacaactacc tcaggaaaaa gaggtggagg aattgtatca acaatttgaa
    25741 cattatctgt ttaaagattt ggtcaagcat tctaccttga aacccgacac attggaaacg
    25801 atcgaacggt tgagaaacgc tgggatcaat attggttcca cgaatggatt taatgctcct
    25861 atgatgacaa tcgtagcaga aaccgcaaaa aaagaaaggt attcaccaga cttcatggca
    25921 acttttgaag acgtcgaagg gtatggtcga ccgtttcctt atatgatcca taaaaacatg
    25981 cagtacttca aaaacaagtc ggttgatgaa gtaatcaaag tcggagatac ggtctcagat
    26041 atccaggaag ggaaaaatgc cggagtgttg acagtaggtg taattgaagg tagttcgcta
    26101 atggggattt cttatgatga gtaccaaaaa ttagagatgg ggaaaaagat cgtttaaaac
    26161 agtctgttgc tcttagattc caacaagccg gcgctgatat ttgtgtaaac aatttaaaag
    26221 agttaacaga atacattttg tacgctgaca tgaatccaga atgactttta tacaaaataa
    26281 gaggttgtga aaaagctgtc ctgtatcaag taataaaaac agccaagcaa aaatagctcc
    26341 tcatattttt cgtttggtta ggcttattac cgcagatgca agcttttgaa caccgtttat
    26401 tcgaaaaaaa aaagggagct gtgacacgac ttttgtcaca gtccccttat ttgcaaacaa
    26461 acagtgttta agagacaacc tctccactaa acggtgttca ttcagctgac tctcggtatt
    26521 tgctcaaaaa acgataaaat ttgaaaagct attttctctt ttgtttctct aatactcgga
    26581 tcagaacgct gatttcacat tctctttttc ttacatctta tctggtgcat gaagtcctag
    26641 caaacgaagg tcttctttca ataatacagt cactgcataa actagagcta agcgtgcttc
    26701 tttttgttca tcatcagcta aaatcttcgt atgagcatag tatttgttga atgcttgtgc
    26761 taatttgatt gcatgtttcg caatcactga cggttcgtat ttttccccag cagacaagac
    26821 cgtttcaggg tatttttgaa ctaatttaac cacttcccag ctggtatcgt cattcaatgc
    26881 atagtctgct gtttctgaag ggacaaatcc tgctttttcc aacaggctga ctgcacgagc
    26941 atgtgtatat tgcacataag gtccagtttc tccttcaaat cgaacaactt cttccaggtt
    27001 aaagtcaaat gtattcagtc gatcgttttt caaatcgtgg aaaatgactg ccccaacccc
    27061 aacttgttta gctacagcat ctttgttttc caaatcaggg tttttctcgc tgatttgttc
    27121 tttagcagat tggattgctt catttaatac ttcttctaat aagacgatct ttcctttacg
    27181 agttgataat tttttaccac cttgcgtgat caaaccaaat ggaatatggt gcatgtcgtc
    27241 agaccagtca aagcccattt ccttcaagac agctttcaac tgtttaaaat gatagctttg
    27301 ttcattccct actacatata gagattgttt gaagtcgtaa gtacgtttac ggtacaatgc
    27361 agcagctaag tcacgtgtaa tgtatagtgt cgcaccgtct gacttcttaa tcaacgctgg
    27421 attcaggtca taagcagata ggtcaacgat ctctgcacct ttatcctcgt tcagcaaatg
    27481 tttttcttct aaaagtttga cgatttcatc catcttgtca ttgtagaaag cttcaccgtt
    27541 caacgaatcg aaacgtactt ccagcagatc atagatttta ttgaattctt tcatcgattc
    27601 atcacggaac cattgccata attgaatagc ctctttatcg ccttcttcca gacgtttgaa
    27661 ccaagcacga gcttcttcat ttaattctgg ttctgtttca gctacttcat ggaattggac
    27721 gtataaacga agcaattcat tgattggttc tgctttaaca gcttcttctg taccccattt
    27781 tttgtaagcg acgatcagtt ttccaaactg tgttccccag tcacctaaat ggttgatgcg
    27841 gattggctga taaccgattt tttccataat gaaaccgatc gaattaccga taactgtcga
    27901 acgcaaatgt cccattgaga ttggtttggc aatattagga gaagacatgt cgattggtac
    27961 tgttccttga ttcccgatgt tgctatcccc ataatgttct ttttctttca ctactgtttg
    28021 caaaactttt ttgctgataa gttctttatt catgaaaaag ttcaagtaag gtccgacaac
    28081 ttcgattttt tcaaagttgg cagaatcgat tttttctgct aaatctgcag cgatttgctg
    28141 tggcgctttt cggtaaactt ttgctaatga aaaggctgga aatgcaacat ctccgtgttc
    28201 agctgatttt ggattttcta gtaattgctc gacttgttcc agtgttaaat catctttgac
    28261 agcgtcatag acagcttttg cgacaagatc tttattattc atctttattc ctcctaaagt
    28321 gttcccttga ttgttaaaca aaaaaaatct ctagcgacgg catacttagt atgccatcac
    28381 tagagacgag atttcccgcg gtaccactcc aattgtccta aaaaaggacc agctccatct
    28441 gatatcggta gacgaccgtc tgtttaaaac agaattctcc aagtgcgctt cgttgtatct
    28501 tcttcattcg gcttccacca cttccgaatt cgctagccaa gaatgataca agtactctct
    28561 tgttcaacaa atcttagtct tattaaactt tcttgaatta tatcagaaaa aaaaacgaaa
    28621 ggctagtcgt ttcataaaaa aacaatccag atggcctaaa ggagaagaaa taccacctgg
    28681 atagctataa atcatttcgt aaatacgaaa gacttaacaa attaatcagc tacgataatc
    28741 gttccttctt cagaagctaa aagattctcg atattttcta aagaagtgat gattgcctta
    28801 gcgtttggtt ttgcttcaac aaatgcaata gctgcttcta ctttcggaag catgcttcct
    28861 ggagcgaatt gcttttcatc aatgtattgt ttcatttctg aaacagtaac agtttctaat
    28921 ttcttttgat ctggtttttg gtaattgaca tacacattgt ctactcctgt caatacgatc
    28981 agcaaatccg catcgatgat ttcggctaat ttttctgaag caaaatcttt atcgattacg
    29041 gcttctcgtc cttctaatcc agtagctgtt tctacaacag gaatcccacc gccaccaaca
    29101 gagactgtga taactccttg atcgaccagc gtttcgatga cacgtgcttc tttaatcgag
    29161 atgggtttag gggaagcgac tacttttctc catccacgtc cagcatcttc tttgaatgtc
    29221 acagtggagt cagcattcat ttgttctttg gcttcttctt ccgtgtagaa tggaccgact
    29281 ggtttacttg gatttttgaa tgaaggatca ttttcatcca caataacttg agtgactaaa
    29341 gaaaccacat ctttatcgat ccctttttcc ttcagtactt cgcccatggc gttttgtaac
    29401 cagtaaccga tagatccttc tgtcattgca acacatgtat ctaaaggcat tgccggtgtt
    29461 ttctcagaat cagcagcttg ctgctgaatc aataggttac cgacttgcgg accatttccg
    29521 tgggagatga tcaattcatc tccttgttcg ataaatttca ctaaatactt tgctgtttcc
    29581 attaacgctt cttgttgggc ttttgcactt gcatccgtag acaagatggc attgccccct
    29641 aaagcgacaa ctacttttcg tttggccatt tcattcgtcc tttcttcagt tctaccgaat
    29701 tttctagata acaaaacgag ccagcgtttt gtgattttcc ctcaatgctg actcgttttt
    29761 tcgtgagatg aatgtggtta tgcttaaacg cgagggataa acaagttacc taatgttgct
    29821 gccatgatcg ctttgattga gtgcatacga ttttctgctt ggtcaaattg acgagcatat
    29881 ttgctgcgga agacttcatc tgttacttcc atttcagtga tgccaaagcg ttctttcatt
    29941 tgttcaccgt aaacagtgtt tgtatcatgg aaagctggca agcagtgcaa gaagatcaaa
    30001 cgatctgtgt tgtgtgtttt ttctaccatt tccatattga tttggtaagg tttcaacaat
    30061 ttgatacgtt cttcgaattt atcttcttca cccattgata cccatacatc agaataaagg
    30121 acgtctgctc cgtctacacc tttatcaaca tcgtcggtaa tcatcaattg tgcaccggat
    30181 ttttcagcaa agccttctgc cattttgacg atttcttctt caggttgtaa ttcttttggt
    30241 gcaacgatac gcatattcgc tcctaaaatt gcacctgtga ctaataaaga gttagccata
    30301 ttgtttcgac catctccgca gtaagcaaca gtgatacctt ctactgttcc aaagttttct
    30361 tggatagtta aaaagtcagc gatcatttgt gttggatgcc attcatcagt caagccattc
    30421 caaactggaa caccagagaa ttctgctagt tcctctacca ttttttgact gaatccacgg
    30481 aattcgattc cgtcaaacat acgtcctagt actttagctg tatcttctgt ggattctttt
    30541 ttacctaatt ggatatcatt tgcacctaga tattcaggat gtgcacctag atcgatcgct
    30601 gcagtagtaa atgcagaacg agtacgtgta gatgtttttt caaataaaag cgcaatattt
    30661 tttccttcta gatagtgatg tgggatacca cgttttttca agtctttcaa gtgttctgaa
    30721 aaatcgatca aatattgtaa ttcttctttt gtaaagtctt tttctgctaa taagcttctt
    30781 ccttggaata ctgattcttt catattaaac attctccttt agattgtttt tatttaagat
    30841 taaagaggaa atcccgacgg cagctgacag taagactgcc gtcaggaaac aaattacttc
    30901 aagtcttcac ggacaagtgg ttggctcatg cagcgtggac caccacgacc tcgtgacaat
    30961 tcacttgaat tgatttctaa cactttgatg ccatagctac gaagcaattc gttcgataca
    31021 taatttcggt tataagtgac tacaacacct ggagcgattg ccaatgtgtt tgatccatca
    31081 ttccattgtt ctcgaggtgc aacgatctca tctccattac cagttgggat cagtacaaga
    31141 tcatctaatc caagagcagc tttcatcgtt tcgtgtaagt tatctgaatg agtaatcttg
    31201 atatcatcac catctggttc gattgtaaag acatccatct taccgtcttt actttgaatt
    31261 gctggatgga tagtgaattt atcgtgatct accattgtaa atacggtatc caaatgcatc
    31321 attgcacggt tgttcggaat tttgattgcc aacacttttt caaatcctga attttttgcg
    31381 aataggtggc gtgctaattt ttctaaagct ttcgcattag ttcgttgaga aatcccaaca
    31441 gctactactt tatcgcttaa gatcaattcg tctccgcctt cgatatgatc tggattttca
    31501 cgatccaacc atacttcgat tcctttattt gcaaatcttg gatgatgttt caaaatatat
    31561 tctgtaaaca tcgattcacg acgacgtgct tcaaaagtca tcttattgac tgtcatccca
    31621 tttcccattg atgcggaagg gtcgcgtgta aagtacaagt ttggcattgg gtccatgtaa
    31681 aatggatagt catcgtctgc tgacagatca tatagactgc tagaacgtac ttcgatatct
    31741 tttgtacgaa ccccagccat gattttatct accatttctt gcgtttccat tgatagaagg
    31801 aactcatgaa gaccctctct tacagcattc gatgcaatat gagattcatc caacatttta
    31861 ttcaagaatt gttctttgac attgcctgcg tcaatcgctt ctgccgctaa tttttccaag
    31921 taaagagtct cgacgccttc attttgcaat gtcttcgcaa agttgtcatg ttcttcttga
    31981 gcaatcggaa gatatggaat atcatcgaac aacagacgat ccataatatc tggtgtcaaa
    32041 ttttccactt cttgtccagg ccgtttcagt aaaactgtct ttaatttccc tatttcagag
    32101 aaaacgtgaa taggtttatc catgttgtgt tgcctccttc tttttgatta catgtatagg
    32161 atatcctcct tttgtaatca ctttcataat tttgtctcat tcaaaatgag acattcctca
    32221 cattaatatt cgataattaa ataattaaac gtttttctat ttttaagcg
//
LOCUS       DADVXU010000019        30978 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.020, whole genome shotgun sequence.
ACCESSION   DADVXU010000019 DADVXU010000000
VERSION     DADVXU010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 30978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.020"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(30..1796)
                     /locus_tag="KB953_000883"
     CDS             complement(30..1796)
                     /locus_tag="KB953_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6865878.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1756..3510)
                     /locus_tag="KB953_000884"
     CDS             complement(1756..3510)
                     /locus_tag="KB953_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6865879.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3620..4189)
                     /locus_tag="KB953_000885"
     CDS             complement(3620..4189)
                     /locus_tag="KB953_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HBB6865880.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(4207..5622)
                     /locus_tag="KB953_000886"
     CDS             complement(4207..5622)
                     /locus_tag="KB953_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBB6865881.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(5633..6301)
                     /locus_tag="KB953_000887"
     CDS             complement(5633..6301)
                     /locus_tag="KB953_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBB6865882.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(6431..7015)
                     /locus_tag="KB953_000888"
     CDS             complement(6431..7015)
                     /locus_tag="KB953_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBB6865883.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(7346..7579)
                     /locus_tag="KB953_000889"
     CDS             complement(7346..7579)
                     /locus_tag="KB953_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HBB6865884.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(7594..8544)
                     /locus_tag="KB953_000890"
     CDS             complement(7594..8544)
                     /locus_tag="KB953_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HBB6865885.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(8541..9383)
                     /locus_tag="KB953_000891"
     CDS             complement(8541..9383)
                     /locus_tag="KB953_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBB6865886.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(9385..10104)
                     /locus_tag="KB953_000892"
     CDS             complement(9385..10104)
                     /locus_tag="KB953_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBB6865887.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            10796..10954
                     /locus_tag="KB953_000893"
     CDS             10796..10954
                     /locus_tag="KB953_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6865888.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            11026..12252
                     /locus_tag="KB953_000894"
     CDS             11026..12252
                     /locus_tag="KB953_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBB6865889.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(12340..12732)
                     /gene="rpsI"
                     /locus_tag="KB953_000895"
     CDS             complement(12340..12732)
                     /gene="rpsI"
                     /locus_tag="KB953_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBB6865890.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(12748..13191)
                     /gene="rplM"
                     /locus_tag="KB953_000896"
     CDS             complement(12748..13191)
                     /gene="rplM"
                     /locus_tag="KB953_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBB6865891.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(13710..14177)
                     /locus_tag="KB953_000897"
     CDS             complement(13710..14177)
                     /locus_tag="KB953_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HBB6865892.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(14297..15835)
                     /locus_tag="KB953_000898"
     CDS             complement(14297..15835)
                     /locus_tag="KB953_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HBB6865893.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            15915..16601
                     /locus_tag="KB953_000899"
     CDS             15915..16601
                     /locus_tag="KB953_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBB6865894.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(16802..17329)
                     /gene="msrA"
                     /locus_tag="KB953_000900"
     CDS             complement(16802..17329)
                     /gene="msrA"
                     /locus_tag="KB953_000900"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBB6865895.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(17413..18090)
                     /gene="pgmB"
                     /locus_tag="KB953_000901"
     CDS             complement(17413..18090)
                     /gene="pgmB"
                     /locus_tag="KB953_000901"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBB6865896.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(18087..19217)
                     /locus_tag="KB953_000902"
     CDS             complement(18087..19217)
                     /locus_tag="KB953_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBB6865897.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(19217..21400)
                     /locus_tag="KB953_000903"
     CDS             complement(19217..21400)
                     /locus_tag="KB953_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBB6865898.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(21397..22422)
                     /locus_tag="KB953_000904"
     CDS             complement(21397..22422)
                     /locus_tag="KB953_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBB6865899.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(22467..23294)
                     /locus_tag="KB953_000905"
     CDS             complement(22467..23294)
                     /locus_tag="KB953_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBB6865900.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(23291..24358)
                     /locus_tag="KB953_000906"
     CDS             complement(23291..24358)
                     /locus_tag="KB953_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HBB6865901.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(24384..25220)
                     /locus_tag="KB953_000907"
     CDS             complement(24384..25220)
                     /locus_tag="KB953_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBB6865902.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(25217..26086)
                     /locus_tag="KB953_000908"
     CDS             complement(25217..26086)
                     /locus_tag="KB953_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBB6865903.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(26156..27439)
                     /locus_tag="KB953_000909"
     CDS             complement(26156..27439)
                     /locus_tag="KB953_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBB6865904.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(27453..29138)
                     /locus_tag="KB953_000910"
     CDS             complement(27453..29138)
                     /locus_tag="KB953_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HBB6865905.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            29413..30438
                     /locus_tag="KB953_000911"
     CDS             29413..30438
                     /locus_tag="KB953_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBB6865906.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
BASE COUNT         9448 a         6199 c         5052 g        10279 t
ORIGIN      
        1 aattgtaggt taatttaaag cctatttcat catcgtgatt gagcttcttt ttcatagacg
       61 aatttctcag atagtttagt tttctttatg agatttttgt atatttctga ccttcgatac
      121 aactcattat gagtaccttg atcaactagt tttccttgat ccatcactac aattttattt
      181 accttttcaa tagacttcaa gcgatgcgat ataattagta ctgtcttatc ttgtattaaa
      241 cgatttaaac tttcctgaat acgtttttca ttttccgcat ctaaactggc agtaatttca
      301 tctaaaatta aaattggcgc atttttcaaa aaagcccgtg ctatggatag gcgttgtcgc
      361 tctcctccag aaagtgaact tccgttttct ccgaccaatg tatgataacc gtcaggtagt
      421 ttttcaataa agtcttcgca gttagctaaa cgagctgctt ttttcacttc ctcatctgac
      481 gcttgactat ttccaatacg tatattttcc aaaatacttg tattaaacaa agttacctct
      541 tgaaaaacaa tagcgatttt gctaaacaaa gaagctggag atacacgttt aatatcatac
      601 ccatcaatta aaatacagcc ttcatcataa tcatacagtc tagacactaa tttcaagata
      661 ctagttttcc cggaaccact agcacctact aacgcagtca cttctccttg tttagccgta
      721 aaagagatat gatctaaaat tggggtattg ttatcatatg agaaagagac atctcgcaac
      781 tcaatgtcaa aagatttcag aggactatct gatccctctt ggatactggt ttccttcata
      841 gctctaattc gctgtatttt aggagctaaa taaaatattt caagcactgc ttctttcata
      901 gaatcgaagg aatcctttat ttttattgcg gctagtaagt aaccaacgac gtagagtata
      961 gtcacttcac ccgttaacag taagtgaaca cctactagaa tgactactgc taaactaaca
     1021 tatgagaaaa tagaggataa ggccatgact gaaaaagtac ttaattctac ctttaaatga
     1081 atcctttcac tctcttccat ttttttgaaa agtctgtctt gaactttttt agatagatta
     1141 aagctattaa tctcttgctg caattctata gtttcttgaa attcttccga gttctctctc
     1201 aaagtatcgt aatatttagt attggctttc gtttgggatt tctttgataa caagattaag
     1261 acaaaactaa ataacgttgg caaaataaca gctaatccca ttttgacatt accaaccaaa
     1321 agcatcactg aaataaaagg gaaaaacaga gccataccgc cggctttagg tattgcatga
     1381 ctcatcgcat gctcaatacc ttcaacgtca gacatgatag tttgagataa atctgataaa
     1441 ttatgttttg aaaaatagga taatggtaaa tttgacaaat cgtctgcaat ttgcactctt
     1501 aaatgcgcac tttctttata agtactatta tataagtttt catactccct atctaacaac
     1561 caatacatga aaagcaaagt aacaaacgat acggcaaaat aaagccaacg gggctttgta
     1621 ttttctaaaa ctaattcgtc tatcagcatc attataatga atgctggtac catattaatg
     1681 cagtatacaa aaaaactaga aatagaagct ttagttaaag cttctgcgcc ctgatcactt
     1741 aaagcaaatt tgtttttata aaaacttttc attgtcaagc ctccaatcat ttgcattctc
     1801 ataattagtg accaattgtt tatacacttg atgtttagcc attaattcat tatgatttcc
     1861 acgttctact attttccctt ctgacaagac aagaatttca tctacagatt taatgctgga
     1921 taatcgatga gctatcataa taactgtttt ttctttcatc agatgttgaa aagccttttg
     1981 tagttcatac tcattatctg gatcaataga tgcactggct tcatccataa taacaatctt
     2041 tgcatctttt aaaattgctc tggcaatagc tatacgttgt ttttctcctc ctgacagata
     2101 aactccttta ctaccaatta aggtatgctc tctttcggga aatttttcta aaatcgattc
     2161 gcaaccggct aatcttagcg cgtcaaatac tttacttgct ggcgcttgag gatttgctat
     2221 agaaacgtta tcgtaaatac tctgattgaa tagtttcgta tcttgaaaga caaaagcaat
     2281 tgtgttaata attgtgtctt ttgaatagtg atttaaaggc ttaccaccaa ttttaatctg
     2341 tcccttatcc acattgtaga aacctgaaat tagtttagct atcgtactct ttccgcttcc
     2401 tgattttcct actaatgcat aaattctttt ttgttctaaa actaagttga aattttctaa
     2461 aacacactct tctttgtatg aaaagctgac attttcaaat gcaatgtcac aatttttaat
     2521 ctgtgtctcg ttcccatatt ttagttggtc ttgcttcatt tctaaataaa gggagtctag
     2581 tgtttcgact gcatatcttg cttcaaacaa gtgcatactt acatacatta ttctcatgaa
     2641 agaaacaaaa ataattccac ttaaaaaaaa caacatcaac aagtcaacag ctaatatctt
     2701 gggagtggta agagaagtta taaaaaaggt tataggaact atcaaaatgc aaactatgcc
     2761 aaaaaatagc cattgaaaaa gaacaaaagg tgttttccca ctcaaagaat acttatatgc
     2821 aaacttagca taatcgttaa ttgctttatt caaggcttta aatgatgtta ctttagcacc
     2881 aaatatcttt ataacttgaa ttccgcggat atattctacc gtttcagagc ttaacacatc
     2941 taacgattct tgatattttt gaataaaatt tgtattctct gtcattttct ttaacaagaa
     3001 tacaccaatt actaacatgg cgattagaac caatccaatt ttaaagttaa tgaaaaagcc
     3061 taaggcaact gataaaacag gtgttactat ggcttgagca ctatctggaa tcaagtgagc
     3121 aattgccata tgtgttttag ccgcgttgtc atcaattgtc tttcgaatat atcccgaaga
     3181 atttaaatca aaaaaacgaa aacttgcttc tgttaaaccg ctaattccct tttttctcaa
     3241 attagtctct aagcgaaacc caaggatatg agaaaataag ccagaaaaaa tataaaataa
     3301 agctcctaaa gttaaccaag caacagtttg aaccgcataa ctcttagcta aatattcatc
     3361 tcctacaata attagagact gaagaaattt gtaaatactg taatatccat agactgttaa
     3421 tacgacagaa aaatcagaaa ataaagtggc taaccatccg tagtatttca ttttagggac
     3481 ataactaaat aattttttgt atactttcat tttttcctcc cccgcattca atttcttctc
     3541 ttattcgttt acaactgtta atgctttccg taatagcgta aattagagat gtacaaacag
     3601 ataaaaggta aactacgttc tattttttgt atttttttaa gacttttctt ccaatcaata
     3661 caccaatcat agataaactt gctacaacta acactgaaat gattccccat atacctaccg
     3721 catagtggat cattgcatcc attgttgcag cagattgtcc tttatctatc caatcttgac
     3781 gaaaacgatc tgtaaagaac catataggaa tccactgtcc ggcagcactg cctaattgca
     3841 ttagagccca agacgcaact tgaggaattg ctcgatcata tccaaataca gatagaataa
     3901 tatcagctaa gatcgccata ctaatcgaac tgataaacca aggtaagtac gccccgccca
     3961 ttaacgtaaa aaataacaat aaaacaattg tgtaaatagt aaaaattcct tttctagggt
     4021 agttatagct aatataaaca aatatgattc cagataataa tccaaaaatc ccgggggata
     4081 tgctgtgacc aaacgcccca ccaatactac taaccattat tgaaaaaaag taaattacta
     4141 gggaaataac tgtagctaaa ccaataaaaa tataatcttt tgttttcata tatattctcc
     4201 tcaattctat ttcgttatgt cattaaaaat ctgttctatc aaagttgaat cttctactct
     4261 aagttttata tcttttttta ttccaccatt ttttaaataa ataattctat tgcatgtttt
     4321 ataaagaaat tccggatcat gagtagctat aactactgca cccttttctt ttaagtcttt
     4381 aataaggtca gcaatccttt ccatattaat ataatccaac ccacttgtag gttcatcaaa
     4441 tagaaataaa ttagtttttg acaatgaagc tatagaaacc aataatcttt gtttttctcc
     4501 accggataaa tcaaacggta attgatcttt cattttataa agttccattt tttttaaaat
     4561 tttttcttgc tgatctttac taactatttc attccccagg gctaactcgc tcctcactga
     4621 ttctgtgaaa aattggtaat ccatttcttg cattactaaa aacggcgatg acaaattttt
     4681 tttattttcg attatctttc ctttggtagg gcgaataatc ttcatgagta ttcttagtaa
     4741 ggttgttttc cccacaccgt ttttccctac caaaccaata acatctccaa ctttgacatc
     4801 aaaatttatg tcttttagaa tattttcata agtaatttct tcaactttta atacatctgc
     4861 acttaccttt tcgatatagg gaacttcaat cgaactatta aaaatgtcaa atttccttaa
     4921 tgcgttattg ttttcagttt ttaacaactg aatagttgta gtttttacag ttctattttt
     4981 aacaaataga acttgatcaa ttaattcttt caaatagtaa aaacgatgtt cagctataac
     5041 aatagtaaat cctttatttt ttaaatcaca aagtaagtca gcaagtttct tagtcgcttt
     5101 gaagtccaaa tttgaagacg gttcatccaa tataattaat ttagagttta gcgataaact
     5161 acatccaata gaaattttct gccgttcacc acttgatagc gaaaaaatgt ttttatctaa
     5221 tatatttgaa agatctaata attcagctaa ttcattgatt cttttatcca tatgctcttt
     5281 tgtaaatcca aagttttcca tcgaaaatac taactcactc gttgtatttg ttgtaaagaa
     5341 ttgtcctcta ggatcttgac ttacgtagcc aatatttttt gatatacttt cttgtgttaa
     5401 ggattgtagc gagttatttt caaataaaat ttccccaacc aattttccgt cataaagtct
     5461 tggaataagt ccattcatta actttaatag tgtggatttc ccactcccag aagaaccagt
     5521 aatcaatgta agactgcctt tttttattga aaaattaagg tcaacaagcg cttgttcctt
     5581 aacatcctta tatgaaaaag aaacattgtt aaattcaaac aattctttca cctcaaatca
     5641 aaatacttaa gaaaaaaata gtaacggaaa acaggatgat atagtctaat aagctcatgt
     5701 ttgttttata gtaagaactt cttttcacag gagattctat cccctttgtt attgcattag
     5761 cggtcatttc ttcagcaatc atagttgcac gtataagaac gggaactaac agaaattcca
     5821 aatattgcag aggctgaaaa aaagacaagc gaatgtttct catttttaaa gattctttaa
     5881 tcattcttat ttcagaacga taagtcggaa agaatctgat cactactgta atacttaaca
     5941 ttattttttt cggtaaatgt attttttcta aagaggataa taattcattg ctagtataga
     6001 cactaaccaa caaccatgag atcattacga ccggcaacat tcgaaataca atatataagg
     6061 atgtcgcaaa aaatatagta aaaaactcta tcttgatagc atcaataatt ttcattacta
     6121 aaaacagaac tgaataaatc aagatgaaat aaatacatgt ctttatttta cgaaacatca
     6181 atactaaaac tgtagcaata atgaaacacc ccacttgaaa tatatttccc tttcctaaaa
     6241 aaataatgac cggaaaaaaa atatttaaag taatcaaggt taacggattg atttttgaca
     6301 ctctttcacc tccaaaaaat aagatcgcac tgttatctta agctgataaa aatgcaccgt
     6361 tattcagata acagtgcgat cttatcaaaa agcataactg agaacaattc tcaatgcaag
     6421 ctttttttaa ttaactcagt aattcaatcc acccaggaac agaaaattga tacaatcttc
     6481 tggttaatag catagtcttt tcttttgaat agctatgctt caatatatca tagaatgtag
     6541 cgtagaatga gtaaatgtag aagtggattt cttcttcatt aaagactaaa ttttgattga
     6601 tcattccgat agtctttaac ttatctagac aacagactgt ctttttcgtc acaaatgatg
     6661 taattttttc aacaatatct tgatatttgg ttccagctga ttggaataaa atcaaatcga
     6721 atatacgttt attctcaaaa atataattaa taaatggatc taattcattg aagttctctc
     6781 tccaactttt ctctatatct atattttcta agtttaagag gtatttggta aactcttcat
     6841 tatacatatt aataagcgca ttaatatgcg gctgaataat ttcttcaaat aacgcttcct
     6901 tagaagtaaa atgtttataa aaagcacccg tagttacgcc tgcggtcagg cacagttgcc
     6961 ttatcttagt ttgctgaaat ccttcttttt caaaatggct tagtccactt tctattaact
     7021 tttggtgagt tataatataa tctctggttt ctatgggtac ttcctcctaa taagatgaat
     7081 taactttatt atcttacaca ggacttgaag tttcaatcgt taacgtttat tactgaactc
     7141 attccttaga gctaaactaa tgagaaagag cagaataact gatacaaaaa gcaatcctac
     7201 aagaattaat agactcatct aaatcattca ccttttctaa aataatccga gaaagattta
     7261 aaaaaattat atccagctat taggataagt cctatcataa tcactccaat agccattttc
     7321 ttatcctccc taaaaataaa gtcttttact taaaaccttt tttgtaatcg tcaataaatt
     7381 ttttcccttt atcagttaat tgaaatccta tatctaaata gtcctcatat ctaagtttag
     7441 ccagcatctc acttacagat gctggactaa cacttagaac ttttgctaaa tcattatttt
     7501 ttaatttctt ttttcttttt gataattcct gaattgctag taaataaatt agtatagtag
     7561 tcgttttaat ttgtaccata cctaccctcc aaattatttc gaaaggcctt cagcaatttt
     7621 atccaggttc catttcatca tcgcatagta actatcacca tcttgtcctt tttctgcaat
     7681 tgaatcagta aaaatcgttg aatagatagg aatattggta tcttttgata ctgttttcat
     7741 tggtctatca tccacactgc tttctacgaa taaggaggga acttttgttg tgcgtaattt
     7801 ttcaactaag tgttttattt gatctggtgt tccttcttct tcagtgttga tttcccaaat
     7861 gtaagcagaa ggcacattat acgctttcga gaaatattta aagcatcctt cacttgtaac
     7921 aatcattttt ttatcattcg gaattgaagc aaatttagat ttagcttctt tgtctagaga
     7981 atccaatttt tcaatatact tatctagatt ttctttatag aattttttat tatcaggatc
     8041 tttttccgct aactgttttt caatattttt agcgtaaata ataccgtttt ctaaatttaa
     8101 ccaagcatga ggatcttcct tccctttctc actctgaccc tctaagtaaa taacatctat
     8161 gccatcactt gctgcaaaat agtctttgtt ttcctctttg ttcgcatttt taactaattt
     8221 tgtaaaccaa gcatttcctc cagtttccaa gttaacaccg ttataaaaaa tcaaatctgc
     8281 ttttgaagtt ttttgaacat cttcaggcaa aggttcatat tcgtggggat ctttcccaat
     8341 aggtacgata ctgtgtaaat caattttatc ttttgctata ttttcggtaa tatctgctaa
     8401 aatcgaatta gtaactacta cttttaattt ttcgttcgaa gccactttat ctttgtcttt
     8461 actatttatt gaacatgccc cgaatataaa aagagcaatc aaaatcccca gacttcccag
     8521 tactgctttc cattttctca tcataacgat ctctccttct ttcttaaaaa tttttgtttt
     8581 ggtgagatta gaaaactaat tacaaataag aatgccgctg ttaaaacaat acttgatcct
     8641 gctgctatgt taaaactata accaataaac aatccaagca atgatgaact tgctccgact
     8701 gttgcagaaa taaatatcat agttttcaag ctatttgcat ataaatatgc tgtagctgct
     8761 ggtgtaatta acattgcaac aattaatata gttcccacac tttgcatagc tgttactgct
     8821 actaaagtta atagaaccat caatagataa tgataaaaac tgacattcat ccctattgct
     8881 tttgccatta atggatcaaa agaagtgatt aataattctt taaaaaatag aactactata
     8941 accaacacta aaaccgaaac gccaattgta atccacttat caatatcttg tacagctaaa
     9001 atatttccaa ataaaatatg aaataaatcc gttgagctat ttgcaactcc tataaggata
     9061 actcctagtg ctaaaaaaga actgaaagta ataccaattg ctgtatctcc ttttattaca
     9121 ctattgcttt ttataaaagt aataatgata gaagctaata gaccaaatac aatcgcacca
     9181 ataaaaaaat taatacctaa aatataagaa attgctactc ctggcaaaac agcatgagaa
     9241 atagcatcac ccataagaga cattcctctt aaaataataa aacaacctac tgttccagaa
     9301 acaatcccta taactacaga tgtaattaga gcattctgta aaaagtgaaa gtcatacaga
     9361 ccattaataa aattttgaag catattattc cccctctcca ataaatatag tatctccata
     9421 ggcttttttt aaattttctt cattaaatac agattctaca cttccgtgag caattagatt
     9481 tcgattcaaa agaataatat tatcaaaata cttctttacc ttgcttaaat catgatgcac
     9541 aattagcact gtttttccct gatgtttcaa tttttttaac gtatccataa ttattcgttc
     9601 actaactaag tctatcccta caaatggttc atctaaaaat atgaaatccg cattttgagc
     9661 aagacatctt gctaaaagta cacgttgaaa ttgtcctcca gataattctc ctatttgatg
     9721 attagaatat tttcccatat cgaccttttc caaagccttt gaaactcttt gccattctgc
     9781 atttttaatt ctttgaaaaa ctttcattcc ggcatatgtt cccatagata cacattcttt
     9841 tacttttatt ggaaaagtaa aatcaatatc agctttttgc tctacatatg caatcttcac
     9901 cagtttttta ttgatatctt cattattaat ggagacctct ccctgatgag ggataatatt
     9961 taacattgct ttaagcaatg tggattttcc agcaccattt ggaccaataa tacctgttaa
    10021 agcaccttct tgtattttta aagaaatctc gttcaatgct aaaaaatcgt tataacaaac
    10081 attaagcttt ttcacttcta acataacttt ctccttctaa atgataatga ttatcacgat
    10141 catatggtat acaaaaatta tattttagtc aaccctaaat tttatagttt agattttttt
    10201 ctgttgctga agattagaat gacaaaacta tttgaaacta cgcgtatagt aagtagcttt
    10261 caaaatatat aaaaaaggag cgttctcaaa tgagaacatg ctcctaaaac tactttattg
    10321 gcgtatggaa aaattatact tgaagtggat agcgggttga ataagttgat aagcatcaaa
    10381 acatttataa aaattattca agactatgtc tgttccatat acaaatatga aaatcagtaa
    10441 actccattta acttttttcg tagtgtgtct ttttatacat gtttatcgtt attaatagtg
    10501 aataggaaaa aaactatttt tgatcattga caattaaata caatttttga ataacgatcc
    10561 ctgctattcg agctagaaac tcaagtacgc catgacctga ttagcgacaa atacttagag
    10621 atttcttttt gttctgggaa aacaaaatgc catgacaata gcagcaaata atgatacttc
    10681 cgtaaggctc gccgtaaact ggggagaggt gaaattccta tgtgcttacc caaagcaatt
    10741 caaaaatttc ataactacta ttttaggaga tgattttata gaatccagag tctacctgaa
    10801 taaaaaacaa acctgtgcat ttctaggaat cgataataat actctagata agtggattcg
    10861 tatatataat ttcccagtaa taagagttgg tggtgtagtt cgctttgacg tagaagatat
    10921 taaagacttt atgaatacat ttaaaataac ttgaaatttt cttctcgccg catatactaa
    10981 aattagtgaa atatactgcc ttactttttt taaggagtga ggattttgcc tacaaagctt
    11041 tcaaatggga aatataaaac taatctgaga tatccaaaaa ggtttaaaga aataacagga
    11101 attgcatcag aaaagtatca aaaaacattt ccaaataggc aattggcgat taaagccgaa
    11161 aatgatatga agaagaaaat agaaaaagtt ttgagagagg agaatgcaaa ctcattagaa
    11221 ttaaaaggaa aaataacgtt taaaaaattt tacgaatcta aatggcttcc tagatatgaa
    11281 ttaggtcaaa caattagatc aaacaggcct cctagtgata taaccattag taatacaaaa
    11341 gatatatttc ggttgcatat tttacctatg tttggtgaat acgcaatgaa ttatctaaat
    11401 atcaatactg agataatatc agatgaatta accaaaaaat cgaaagaata tgcaaatata
    11461 aaaatcataa aaggatatgt tcgttccatg ttcgatattg cagaaatact aaattatatc
    11521 gaatttaatc gaactactaa aattattcaa agtatcactg cacctaaaaa aaatgcttta
    11581 gaagaaaaaa gaatacaaga aggtaagcaa gcactatcat caaaagaact tactaattgg
    11641 atagaagcag taaatgatga ttttaataat catttactta cttttcatga ttacacactt
    11701 tttatgatta ccctgtatct tggtgataga aaaagtgaga cttacgcttt gcaatggaaa
    11761 tatattgatt ttgaaaaaca aacggtccgt cttaaacata ctttagataa atatcaaaga
    11821 aaaaagttta caaaaggtcg aaaagatacc gtcattcaag taccagaagt tgtaatgaca
    11881 ttgctaagtg aatggaaatc ggtacaagct gatcaattgt tgaaattaaa aattaagcaa
    11941 acgttagatc agtatctctt tacttatact aaaccatctg gagaagtaaa ttgtcctgtt
    12001 catgccgatt acctaaacta tcgaatcaat tcaattaaaa gaaggcatcc tgacttagtt
    12061 catttatctc cacacaaact ccgtcatact tatgcaacta tagctagaca aggtggagca
    12121 gatatgaatc aaatttcaaa tgctttaaca cactcagata tttctacaac aaagatatat
    12181 gtgaacacac cagatatagt tgataaggct gtttttgaag cttttcagag aggattgaat
    12241 aagtgtgatt gatatgtgtt cttaaatgtg ttcttaaaaa caaaagaagc actttccaaa
    12301 taattgaaaa gtgccagaaa cgttgatata taaaggatat taacgttttg agaattgtga
    12361 agctttacgt gctttcttaa gacctggttt tttacgttcg accatacgtg cgtcacgagt
    12421 aagtaagcca gcacgtttaa gagctaaacg gaagtcagga tcaacttcta gcaatgcacg
    12481 agcgatacca tgacggatag ctcctgattg tccagcgtat cctccgccgt ttacgttaac
    12541 aaatacgtca tatgcgcctt ttgtttcagt aacagcgaaa ggttgattga ttacttcacg
    12601 caagtcagca tgtggaatgt attcttcaac atctttttta tttacagtaa ttttgccagt
    12661 tcctggtact agacgtacgc gagcaactgc gtttttacga cggcctgtgc cgatatattg
    12721 aacttgtgcc aatgaaattc cctcctatta gattaagttt gtgatgtcta aaacttctgg
    12781 ttgttgtgct gcatgtggat gttctgctcc accgtatacg ttcaattttg taaattgttt
    12841 gcggcctaaa gtgtttttag gaagcatacc ttttacagaa gtttcgatca aacgacgaga
    12901 atttttagca cgcaattcgc cagcagtgat tgatttcaat cctcctgggt agttgctgtg
    12961 acggtaataa attttgtcag tcgctttttt acctgttaat ttcactttgt ctgcattgat
    13021 gacgatgacg aaatcgccag tatccaaatg aggtgtgaaa gttggtttat ttttaccacg
    13081 tagtacagat gcaacaactg ttgaaagacg tcccaatgga acatcagttg cgtctactac
    13141 ataccattta cgttctactt cgcctggttt ggccatatat gttgtacgca cgttattttc
    13201 ctccaattta agttctgatg tttgacatac acttcgagtt tccggggctc tttgtggggc
    13261 aaacaatacc atttaacata ttacccgcta atctagtcaa tgtcaacgtt tttctgcttg
    13321 attgaaaaat ttatatgaaa ctttacttct tttctttctt ttatataaaa gggtcgtttc
    13381 ctgtcaaaat gaaaaacaaa aagaggctgt gacaaaagtc ttgtcacaac cccttatttt
    13441 gaataaacgg tgttctcctg ccaactcctc ggtcacaagt cacaatatcc ataaaacata
    13501 agaagctgtt ttttaaaatt gtttcttgtg actcggagct gaacggcagg cttacaacct
    13561 cttggtaaac ggtgttcaat cagctgaccc tcggtattag ctcaaaaaac ggcaaaatat
    13621 gaggaactat tttgacgttt gtttctctaa tactcaggtc aaaacgctga tttcacaacc
    13681 tctttttcgg tcaagttcaa caacagtttt tacgcgtcga tctctggcgc tttgcccaaa
    13741 tgagcttgga tcatccagat acgtttttct gtggctgttt tgaattcgat aaagatatct
    13801 tgtgttggat cgtcgccttc ttctccagaa acttcgattc ctttttggta aagatctgct
    13861 agataacgat agcctgcaac aagtgtttcc aaacgttctg gaatagtacg atcccagcgg
    13921 ccaatttcat cagggatttt tgtgttatct gcaaattcac gcaatgtaga ataaggtgaa
    13981 ccgtctaatg tgatcaagcg ttcggatact tcatctaact gagcgttcaa atcatccatg
    14041 aactcatcca tcattgggtg caatgtaagg aaacctggtc ctctcatgta ccaatgtgtt
    14101 tggtgaacaa caacagacat ttggcttaga tctgcaacta attgatttaa tacttctttg
    14161 gtttgttgga atttcatctt tcttgcctcc taacattttc ttacacttaa aataatagta
    14221 gactttcaat gaaaagtaca acaaaatcat ttacctgaga acgattttca gttcgctttt
    14281 ttatccgtaa agccaatcat ttttcagaat cagctaattc ttctcgcagt tgagcaaatt
    14341 gctgataagt ctgtgctaaa tgcttttgtg tcgaaacaaa taatggatag attttctgat
    14401 attttttatg atcagtttct tgtactccaa cttgctcttt caattctatc aattcctgtg
    14461 cttcactgat actttggatc tttccgatgc ttctccagcc taaaatcaca gctcctaatg
    14521 cggatgcttc tgtgacagta gggaaaacaa gcgggtaacc cagtacatca gccatcatct
    14581 gacgaaaaat cggagattga gcaaacccgc ctgtagcacg gatctctgtg agctgatttt
    14641 cctggcaaac tccttttaaa atccgataaa ggttgagaca gatcccttct agaatcgcct
    14701 tcatcatttc tttttctgta tgttgccgct gtaaaccgat aaaactgcca aacgcatctg
    14761 cgtcccaaag cggtgctcgc tcaccaagta aatagggatg aaaaatcaga ccattggcac
    14821 ccgctggcac aaaagcgata gccgaaagca gtgcatcata tggattttct cctttttctt
    14881 tagcagattg aacgacttcc tgtaaaaatg tatgccctgc ccaatcaaag atgccagcac
    14941 cattgctagt cgctcctccc actacccaat gtttctgatc cagaacataa caaaacgtct
    15001 ccatttctgg atggaactga ggctgagtag taataaaacg aagtgctccg ctcgttccta
    15061 cggtcaaagc ggcgatcgta tcaccaaaag cacctaaccc tagattagaa agcggcccgt
    15121 ctgcccctcc caaaacaaat gatatatcat ttggcaaacc tagttctgct gctgtttttg
    15181 atggtagacc tgttacttga taggtggaag gaaagacttg cggcaattgc tctattcgaa
    15241 tgttcaagta gtcaagcact tcttctgccc attggaattc atggatatca aaatatccag
    15301 ttcctgaagc tgttccgtag tcgctgatca gttcgccaaa caatctataa aagatatagt
    15361 ctttgatacc gacaaaataa gcagtttttt tgaataattc aggttggctg ttttgcagcc
    15421 acttgatttt gttcaatggt gtcattggat gaatcggcgt cccagtctgc cgataaaatt
    15481 ctttgccaag cacggtatat ttcagttttt ctgcttcgtc cgctgctcgg ttatctgccc
    15541 aagtgatcat tcgagataac ggtgttttct cttcatccaa caatatcagg ccatgcatcg
    15601 cagaggagaa ggagacaaat tgaatgattg tgtctgaagg caactgttta attacttctt
    15661 ttataacggt gcagacacct tgaaagatca attcctgatc ttgttctgcc atcccttgtg
    15721 tttcttggat caaagggtag cgaaaataag cggatgctgc ttcttgggct tcatctgtga
    15781 aagcaactgc tttggtttgt gtggttccga tatctaatcc gattgaaacg ttcatttttt
    15841 cacctcaaat tttatgtatg tacaaaaatt tagtttaagc ttgcgtatct tggttttgaa
    15901 aagggggaga tggcgtgtta ctttatttta tcattggttg ctgtatcgga tcatttttat
    15961 gtttaaccgc ccagcggatt ccgttaggtc actctattat ttatcctaga tctcattgtg
    16021 tcaaatgctg tcgttcgctt tcttggtatg aattgattcc gattctctcg atcatcgtcc
    16081 aacgatttcg ctgccgctat tgcaggtgtc ggttgccctt ctactatttg ttggcagaag
    16141 ccttatgtgg tggtcttttt gcctggttct ttactttttc ttccgcaaga aatttttcga
    16201 cgttcatatg gatgctttcg gctttgctat tttcgttgat ggatttattt tacttcatgg
    16261 tccattctca cacgctattc tgttcttggg caattttatg gattttttgg ttgcaggccg
    16321 gcgtcttcca atggcaaagt gtgctgttaa cgtttatcgt aggcggtgtg tgccttcgct
    16381 atggtcaatc ctatctcgga gctggcgata ctctgttgat gctttcttgg agtggtggct
    16441 tgtctctaga agagttgctt cagatcttgt ttcttgctag tctgtttgga atatccgttt
    16501 tttctgttta ctttatctgt cataaaaaaa agttggaaaa gctgcctttc attccttttt
    16561 tgagcagtgc gttgctgatt gtccttcatc ttaggtattg aaaaaaggtt gtgacaaacg
    16621 acttgtcaca acctctgata tatagtgttc tcttaccaaa tccttgtgac ttggagctag
    16681 gcgacaggtt cacaacctct ccactaaatg gtgttcaatc agctgaccct cagtattagc
    16741 tcaaaatgct ggttttacaa cctctgatat acggtgttcc ataagtagct ttttcggctt
    16801 cttatttctt gacgccccaa tgtcgacgaa ggaaacctgc acgtccagaa ccttctcgat
    16861 aaagattgta atggagcttg tttttcttgt aataatcctg atggtaatct tctgcaggat
    16921 aaaattcgct tcttggttcg atttttgtca cgattggctt atcgaatcgc ccgctgtctt
    16981 gcaatgcttg ttttgaacgc tctgcaatat ttcgttcttc ttcatcatta tagtagatca
    17041 ctggacgata agaatcaccg cgatcagcga actgtcctaa tgcgtcagtc ggatcggttt
    17101 gctgccaata gatttccacc aactgatcat aagaaataac ttctggatca tattcgatct
    17161 ctactgcttc tgtatgccct gttgtgcctg ttgttacttg ttcataagtt ggatttggta
    17221 catgcccgcc agtataaccg gatgtcacag aaatgatccc tggctgctct tcaaatggtt
    17281 tgaccataca ccaaaagcat ccgcctgcga aaactgcttt ttttgtcata taaaatcccc
    17341 cttttttctc atcatacctt gatttccaaa aggaggataa cgttttgctt gtgacagaaa
    17401 aaacggaaac tattatatac gtgatacggc ttctgataaa tgccaaaacg aacgtacttg
    17461 atcaaaaacg gttaaatcag gaatcacata ttcagcagaa ggaagattta tgttttcacc
    17521 tattcccaat acatgcatgt taccagccaa agctgcttga caaccagctt ctgaatcttc
    17581 aatgaccaag catgcatttg gcggtacatt caattgctgt gctcctttga gaaaaacttc
    17641 tggatcaggt tttgcttttg atacttgcgt gccgtcaata aggacatcaa aataacttgt
    17701 caaattcgtt ttttctaaaa tgaggcgtgc atttttgctt gctgatccca atccgatctt
    17761 gatgtttgtt tttttcaagt actcaagaac agctattgca ccaggcaaaa cggatgtttc
    17821 atccattttt tgaatcgctt gaagatatag attgtttttt tgctcagcaa aggctgattt
    17881 ctctttttct gagaatttct gttccatttt tccaaatgac agcaatcgct ctagtgactc
    17941 ggttctggat attcctttca gttgctcgtt ttctttctca gtgaatggaa tggacagctg
    18001 gttagctaaa accagccacg cttcgtaatg ataacgagct gtgtctacaa gaacgccatc
    18061 taaatcgaat agtgctgctt tgattttcat cgattacctc cttgaaaatg aacgacttga
    18121 agtaaagatg cgatgttttt agctgtgcga tgaagattat cagtggcttg ctgtaaagct
    18181 tcttgtaaag gcacagctgc aggaaggatc ggaaaaacgg ccgaaatgcc aacatctgct
    18241 gctttttcgc ttcctttacc aacacttccg caaatagcta tcacaggaac gccctctggt
    18301 actcttctag caatcccaac tggcgctttt ccttttagac tttgtccatc tagacgtcct
    18361 tcgcctacga ttacgaggtc tgcttgctgt acccgtttat cgaactccag taaatccaat
    18421 acgtagtcga tgccagagac tattttccct gaagcaaatg ctaacagtcc tgcacccatt
    18481 ccaccaccag cactactgcc cgatgcctcg attacttgag gggcaaatct catataaaaa
    18541 tcatgcatgg cttggtcagc cactgctaat tcattttctt tcaagccttt ttgtggtccg
    18601 aaaacaaaag aagcgccttc ttttccgcat aaaagattag taacatcagt aatgacagtg
    18661 attttggctt taatcagctc aataggcacg ttcgttttat caactgtagc aattttacct
    18721 agattttctc ccagtgcttc tactttttga ttatcttgat cgtaaaaagc ataacctaga
    18781 ccttctgcca tgccgatgcc gccatcgttg gtactacttc cgccaacgcc gatgatgatg
    18841 tgtcgaacac cttgttgcaa caattgtcgt atcagttctc ctactcctcg cgtggataac
    18901 ctaagtggct gttgttggtt tagtgggaca tctttcaatc cgacgatttc tgccatttca
    18961 aaaatcgcag tttgaccgtc tgaagcgtac tggatttctt tgtttttgcc aaatgcatta
    19021 gtaacgactg ctgtttgccg cttcaacctt aaactgtctg ctaatgatga aaccgttcct
    19081 tctcctccat cgcctatagg tagcagatca tattcagcct gaacaaaaac ttcttgaaaa
    19141 cctgattgaa tggcttgtgc tgcctctaag gctgtcagac tttctttgaa agaatctggc
    19201 gcaatcagaa tacgcattat tttcccctcc gttccaagat cagttctttt tccagctggt
    19261 attggtctcc attgacaaaa ataggtaatg tttctttcgt ctcttttttt attactattt
    19321 gtttttttgt caaaacaaat agcaacctgt gatttttcca aataaaagga aaagaaattt
    19381 tttcccatgc tgaaggaagt cttggagaaa tagaaagagt tccattcgtt actgtaatac
    19441 ctgaaaaacc aaatataact gctagccata gtgctcctaa tgaagcagca tgaattccgt
    19501 catctgatga atgcggatct ggtcctaaat caatcaggca cgcttcttgg aataaatgat
    19561 acgcccattc catttcttgg atacggcaag cctctatggc gtgaatcgct ttactcaacg
    19621 atgagtcgtg gattgttttg ttttcatagt aatgcaaatt cttagaaacg attggttgag
    19681 aaaaatactc aggtaataag tataacagca tgacaagatc tgcttgcttg ctgacttgga
    19741 gttcatttac ttcgctacgg gcataatcaa gtaaaataga ctggctgcca ggacgttctt
    19801 tgtaagtggt caaatcaata actggcttac tgagatacgt gtcatcttgc ggaatcagct
    19861 gttctttgtt aggcactggc aaatataact tatctaaaaa attttgacat tctttcaaaa
    19921 aaagcggatc gccagcttga tatttaacag ctaaggccac attataatgc gccagatagt
    19981 tcgtatatgc attattgtcg atatattctg tgtattcatc tggaccaatg actctgtgta
    20041 tctctaaatg atcatcgact tttacagctc tgctcagcca aaaagctgct gtttcatgaa
    20101 tgagcgtttt tccctttgtt tctaaaaacg attgatcctc tgttgcttgg acatactctt
    20161 gaacggcata agcaaggtct gcactgatat gatgttctgc ggcagctgaa gcaacttctt
    20221 gtcttgttcc ggttttgata ttcaatgcag caaatttggg tgtctcttcc tgaccagtgt
    20281 gtgcgctttc ccatgggaaa agtgcacctt gatagtgata atctcttgct ttttgccgtg
    20341 cgccttctaa tcgatcataa cggtacagca gcaaattctt tgctacttca ggaaactgat
    20401 ataaaaagaa aggcaggata aaaatttccg tatcccagaa aacatgccct ttataacctt
    20461 cgccagttag tcccttcgcc cctacactaa cacttggatc attgcgggga gtcataatct
    20521 gtaaatggta agcagcgaag tccatcgcta actgatcgaa agaagaatcg ccttggatcg
    20581 ttatccgatg ctgatgccaa aaatctgtcc aataagacat cgactgctct aaaatttctg
    20641 tataactaac gcttttctgg gcttgggcaa tacttttctt aaacgggtct gtttgctgaa
    20701 gagaggtaga gagcacgatg atcttctcca gcgtaaagga cgtatttttt ctgatctttt
    20761 ctgaaaagga agcggtgatc cggcgatttt ttgcttggaa tacacctgct ttattgaatt
    20821 tagctgcaat cccaatatca attttacttt ctgttgtctg gtaggatgca ctgatcaaat
    20881 cgtcaccaaa tactcgcagt tctttttcaa ccaactgctg tgtaccaaaa ttggtttgct
    20941 gtccatcgat acccgttttc acatctactt ggcagtcttc ggtcaacggc ttgatgacca
    21001 ctttcagggc tgcctgatgg agattatttt gatcagcaac acgacaagta atgacttcca
    21061 ctttttttcc ttgagcagtt tcaatgagcg catagcgttt aagctctccc atttctaaat
    21121 ctagtgcccg ctcgtatgca agaactttca tcgtctcaag agaaaaatgt tcaccgtcga
    21181 tttttatttc ccatctgatg acatctggga gattcactaa ctcggctgat tctgctccta
    21241 aaggtcggtt atatacacca gctgcaaaca ttccccttac ttgattccga tagtcttcct
    21301 cctggcttcc tctgatacca aaaaatccat ttccaatggt catctgactc gcatacttat
    21361 tgatatactc taaatcaaac tgtttttctt ctactttcat aaggtaataa cttctcctgt
    21421 ttctgccgaa cgatataaat tttccaccag attctggatg acatatcctt gccagccatc
    21481 tgcaatcatc tgagtctggt caccgagcgc ccgattcaca aaatgagcca tacttagttc
    21541 atgtgggtca ttaactggcg tggtcttttg taaggaaacc agctttccat ctacatcttc
    21601 gaatagttcg cctggataca gattagcacc tgcctgatcg ccacaaaaag ccacattcag
    21661 tttgtcttct ctgatattca aagcaaacga agtattcagt tggatgatct tgccgttttc
    21721 taattccagt gtgccaaaaa ttgcatcttc tactgtgaat ttgcttggat cccatttccc
    21781 gaatttccct gaattttttt tcgtaccaat tttttggaag ctgtacgccc gaacgctttt
    21841 tactttagga aatccagtaa tatacatcgc tgtatcaagc atgtgaatgc cataatcgat
    21901 caaaggcccg cctccttgaa gttctttgtc gataaacgtt ccccagcctg gaacaccaga
    21961 ccgacgaagc gcattcgctt ctacgtaata aatatccccg agaacatcaa gatgattctt
    22021 gatcattgtt gcttcgtcag aaaaacgatg ctggaatcca taagccaaaa tctgcccgtt
    22081 tttatcggct gtctctgcca tctttcttgc ttgatcagct gtcatagctg gtggtttctc
    22141 gcacatcact gaagcattag cttcgagggc agccaataca tgttcgaagt gaaagcgatt
    22201 gggtgtacag acactcacga tatcgggaga aatctcattc agcatttgtg taactgatgt
    22261 atacgcatga gaaaatcctg tacgtcggca aaagtcttct gctcgctggc tgactggatc
    22321 aaccacagcg gccaattcta agacatctga tcttttttga taataagcag catgtacttt
    22381 ttctgctact tgtccagcgc ctatgattgc tactttcttc atttatttct ccctcctttg
    22441 cttatttact tattgttatt ctttctctat tctttctcta aatcatttaa atgacgcgct
    22501 gtttccagac agtttttcaa gtattgcaat gattcctgat aaattttcac ttcattttct
    22561 cctcgcaaac gacattcata taccatataa ccttcatact cattttgaag tagcgtttga
    22621 aaatggcgag taaaatcaat tgcgccagat cctggttggt agcgatgatt atctgctaaa
    22681 tggatatgac ccacatactc tttttgcgta gtcaatgctt ttgaaatatc atcttcttca
    22741 atattcatat gataaaaatc agcgatgatc tgcacatgtt tcaattgatt agagtcaatg
    22801 taatgacgcg catccgctaa caaattgatc atatggtctt ggtatcggtt caaaggctcc
    22861 aaataaataa ccgtacctgt ttcttcggct actttatcta aataacgaag tgattcgctg
    22921 actgctttat agtctccttc tttgcttcgg ggtgaaacca tcggcggtaa tcgataagtg
    22981 aacattcccc aagctgctgg gataacgatt cctttgcctc caacctcgct tagtgcacgc
    23041 aaaatctcag caatttgacg tacaccatta agacgccgtt cttcgataaa gtcaccgatc
    23101 cacccatcgt agccgttaca agctgtcact actgggatcc ctgtttcttt gattgcctgt
    23161 ttgactgtgt cgatattttc tattaacaac ttaccgtcga tttcgtattc ttcaaatcca
    23221 cagtctttga taaatctaaa tttttctaaa atgtctcttg ggaaaaatgc ttgatcttgc
    23281 gtaccgaatt tcattgttct cctcctcgga cttgaacgcc cattttgata ctttcttctg
    23341 ggtgctggtc tacataacgc atgtaagctt ctgcgctttc tgcaaatggc acaactggat
    23401 caatgatttc ttcacaatcc aagtaaccgt tcatcaataa ttcccaacat gtgtcctcga
    23461 ttcgtttacg gttccatctt gggtaatctg ggtttggttc actagatgct cgagcaaaga
    23521 cgatttttgc attattgaaa tgtgcttcgc gaccaagatt aaacccagct ggaaaaggtt
    23581 tagcaaaggc aacataagaa atgatcccgc catagccgat ccctcttaaa gaagcttgta
    23641 atgcggcagc actgccactc gtttcaataa ctgaatctac tccacgttta tctgtcaatt
    23701 cctttagacg aaaaccgaca tctgctttca taggatctag cgtgtaatcg gcaccgtatt
    23761 tttctgctaa ttctctacgc tgcgctatgg gatcgatccc aacgatggta gtagcacctg
    23821 cttttttagc taattgaatc gcaatttgcc cgatagcgcc taaaccgaag attgctacag
    23881 catcacctgc tcgaacatgt gcttcccgga taccacctaa agcgaattga gcaggatcgt
    23941 aacatactgc atttttccat tgatccaacc gatcaagttt acgtagttta taattatcaa
    24001 caccgtttgt aatcagcgtt tcacgaatgg gtccataagt gcagactaac tctccttttt
    24061 gatactctgt caccaaactg cccgtttctt cgatcgtacc gacaatcata tttcctaatt
    24121 gaaattcgcc aaataccaca ccgcgttggc ttccttcttc tcttggcaag aataggcgcc
    24181 attcttctga aaattcttca tcgataaatg gacttaatcc tctaaagtcg acaatttctg
    24241 ttcctggttt tggagtagcg aaaattactc ggatacgtac ttcgttcttt tcgatctcac
    24301 ggtcttcgta atctttgaat ccggctattc ttggttctaa tgcaactaat tttttcatct
    24361 tttctttcct ctttcttttc tttttatcct tttacgccgc cttcgactaa atcaccttta
    24421 ataaatcgtt cagataacgc atacataatg atgactggta aagcggtcac caatgaagcg
    24481 gccatcattc gtccccaaat gtagtctggt gtactgaata atgtgttcaa accgattggt
    24541 aaagtaaatt tttcggaggt cgacaggaaa atcgaagcaa ataaataatc attccacgaa
    24601 accataaagc aataaacgaa gacagaaatg attccagaaa tagccaatgg tataatgatc
    24661 cgaataatga tctggatccg gctaagacca tcgatcattg ctgcttcttc aatatcagtc
    24721 ggaatcgtgt caaaataact ttttagcatg aatatcgcag taggaagtgt ttgaacgacc
    24781 atcgtgataa tcagtgctgt attggtatca tacaaaccta gaccggaaat gattttaaat
    24841 aatggaacaa cgatcaaaat accggaaaac atataaacgg tgtaaaagct agcattgatt
    24901 ttcgagcgag ctttgaagcg tagttttgac aaagcgtatg ccccgctgat cccaatcact
    24961 actgcagtta cggaggctat caaggagatc actaaactat ttttgaaata catgatgaat
    25021 ggaaaaatct cagcattgaa aatatcctga taatgttcaa aagtccattc tttcggaaaa
    25081 atagtttgtg tcgttgcaat tgcttcttgt gaatccttga atgatgtcat cagcatgatc
    25141 aaaaatggaa acaaattgac agctaatagc aacaaaatcg tcgcgtaaaa taagattttc
    25201 ctgctctttt tatttttcat ttcaagtcca ccttctttcg tgagagcaag acgaccgctg
    25261 caataatgat gaacaataga atcgatatag ctgctgcttt tccaagatca ttgaacgcaa
    25321 aggctgtctg atacaaataa acgcctaaga tattgacttt attcgtcagt agatagacat
    25381 ctgtaaacat atagaacatc cagatagatc gtaaaacaat cacagttgct agtactggtt
    25441 tgattgccgg cagtgtcacg atcttgaact tctgccaagc atttgcaccg tccatttcag
    25501 ctgcttcata aagcgttttg tcgactgttt gcaaaatagc caaaaatgaa ataaacgcat
    25561 acggatagta tctccagata gcaaacaaaa cgaccataaa gaagctagca actggctggt
    25621 cgaaccacag cggtgcttcg ttgaaaagct gcaacctatc tactaacaga tcattgacga
    25681 ttccataact attattgaac atatatttcc aagcaaaaat caaagaaata gatggcgtaa
    25741 cataacttaa taaaatcagt gaacgcaccg ttttcctaaa tctgaacttg cggttaagca
    25801 aaatagctac ggctaatcct aaagctgtac tgcctgctac aactaagaat gtgtaagcaa
    25861 ctgttaaacc taaagaacga taaaattcac tatcacttaa aatttcaaga taattgctta
    25921 atccgataaa aacggcgtct gagttaggat tcagcggcat atcgaaaaaa ctgatctgaa
    25981 tattggaaag cattggaatc agcactaaaa cagctaataa aagcatgctt ggtgctaaaa
    26041 gtcccatggc taattttaag tcagaatgtt tccttggacg attcattgaa acctccccct
    26101 gataatctgc caatagctat ctactttttt cgaacaccat tggcagaact tcttgttatt
    26161 tcgtattttt ttgtgcgtca gctaaagctt gatcgacatc ttcgtttccg acagttactt
    26221 ggttcactgc ttttccgatc acacctgagc ttgtgatatc tcccattttc gtgaaattct
    26281 tttccccgac tagtccaaag acttgtactt catcaaaagc actagcaatc tcagatggca
    26341 gctcgccaaa agacttgatg acttcatttt ctttgtaacc atccagttcg actacttgtt
    26401 tattcactgg ttgagcacca ccaggagaca tcaagaccca cttttccatg tttttcggtt
    26461 gagataagaa agctgtgaat gcttcagcag cctctttttg tttctcgtct agaccagaag
    26521 aaatcgtcaa tccagatact gtgccataga cagcttgatc tttttgtgtt gggatagcaa
    26581 aaccgatatt ttctgagttc cccgcttcat aaacagaagg caaaatgtaa gttgaataga
    26641 ttgccattgg tgctgttcca ttcatgaaag catctttgat ttctgtcaca tcattagagc
    26701 ctggcatcgt gtattgggat aagttttgat aaaacgacaa tgcttctctc atcttttccg
    26761 tatcgatcgt tacctcgcct tcatcatcca aaacatttgc tccatttgac aaagcaaatt
    26821 gagagaacgc ttgctcactc attgttcctt cagcagtagg catagcaatt ccatattgtt
    26881 tattctcttt atctgtgaaa tgttgagcaa tttttaaaat atcatcccaa ttttctggtt
    26941 ctgaaaaacc agcttctgat aatttttctt tgtcatacca aataccttga acccagccgc
    27001 taattggtgc agcaatatag ctttttccat cttctgaacg aacaaggttt ttggctccgt
    27061 cataataatt atcttctcca gcttcctcga taacgttttg gactgccgtc tgatcaatca
    27121 gttcatcttt atccatcact ttagcaaaat cctgactgac ttccattact tctggtaatt
    27181 taccggaacg agctaacgta acgatttttg tattgaacgc tgattcttct accggcactt
    27241 gcttgatttt gatgtctgga ttttcttttt caaaatcagc aaccagttca ttgatcacac
    27301 ttaatcgttc ctgttcgaca gaagagtgca tgaattgaat cgttgtctgt tgtgattcgt
    27361 tagagctttg actttcacca caagcagtaa gaagaaatcc agtagctagc agacaggtag
    27421 taacaataat ttttttcatt tttttccacc tcttatttta ttattttcag ccacatatat
    27481 tcatacggct ggaatatcaa ctttcgttct tgactgcaat ggataatttg tccagaaaac
    27541 aggtctaggt aatctccttt tggtaaaaga taacctgctg attgattgga aaaattatgg
    27601 ataactaaga ctttctcttc cttcgcttcg cgaataaaac tgataaaaga atctgctgag
    27661 tccagtaagt aaatcaaact attcggatgg aaggcttttt ctttttttcg aatagatagc
    27721 atctgggtca attcaccata tactgctgtt cttaatggat ctgtctgcaa ctctttctca
    27781 atatctgcta acaaaaattt ttgccgattg atttttcgat tctctcctgt tttttctacg
    27841 cctttgtaat catttcgact tcccagcaca ctttgaatat aaattgccgg cacgcctggt
    27901 aacgttacca aaaatgaatg agcagctaaa aagcgtttgc ttctccattc cgtcgtatct
    27961 gttggagaac ttaacgcatc tagatacgta gcgtttatct catatggact ggtcgttcca
    28021 tctgggtttt tcttgtagtt tactcgggta ccctctttct tcagctgttc cactaattca
    28081 aggatcgttt tttccggcag aatacctcgt accggattca atccgatacc gtcatgggag
    28141 gctaaaaaat taaaaaaagt tgtttttttc gatgcgaaat cgatggtttt taaccattct
    28201 ttcaacttac ttgtattacc cgaacgaatc gcttgaaggg tcaaaggtgg tagcgaaaac
    28261 tgatagacca tctgtgcttc attctcgcca ttcccaaaat aactgatatt atctttgtgt
    28321 ggcacatttg tctcagtgat caataccgtt cctggagctg cttcatcagc gattgcacgg
    28381 aataattgga tgattttatg cgtttctggc aggtgtatac aagaagttcc tagttctttc
    28441 cataaaaatc ccacagcgtc caagcgaata aatgaagcgc ctttttctaa gtaaaacaaa
    28501 agaatatcga tcattttcaa taaaacttct ggatttttaa aattcaagtc tacttgatct
    28561 tcgctaaaag tcgtccatac gtgtttttct ttcccgtttt tgtcaatgaa cgttgtaagt
    28621 aacgggctgg tccttggtct agtcacgctg cttgtatctg tatccagtgg caaatcaata
    28681 aaaaaatctt tgtacgtatc attcaagttt aaatattcct gaaaccaagt actttcggca
    28741 gacatatgat tgcagacaaa gtcgaacatt aaccgtgttt cgcttctcat ttcttctata
    28801 tcttcccatg tgcccaaacg gtcgttgatt gcctgataat ttacaacgga gaaaccatca
    28861 tcagatgtat atgggaaaaa tggcaagaaa tgtacaactc taaatgtatc tgccaaatac
    28921 ttttgataaa atttcttaaa tgtacgcaaa gttggttgct gctcttcaaa aaattgatcc
    28981 ccataagtaa tcaagaaaat atcttcttcg tcccaatgca gtttgcctgc ctggcctttc
    29041 ttctgctgat aaacactgac tttttgttca atcatagagg cggttttctc agacaaatct
    29101 gagtcgcgat aaatcgcttg taaaagtttt gtatacatcg gattcccctt tcttttttgg
    29161 gagcgttccc aacgatttga tcatattatg gaaaacgctt actgtcaata gcattttttt
    29221 aaataatttt gatatacaca aaaatacaaa taagaaagct tatgtatcaa taattaatag
    29281 cttcttaaaa aaatatggtc tctcatgatc atgcaagcta ttcctaaatt ttcaaaatca
    29341 tagacaaaaa agaatgtcta ttgtactatg gaattattct aaaacgctcc caaaaaggag
    29401 gtgcatataa aattgaaacc tactatttat gacattgcga aaaaagctaa tgtctcaaaa
    29461 tcaactgtct ccagagtatt aaataaccag ccaaatatct ctgataaagc aaaagcaagt
    29521 gtacttcaag caatcaaaga attgaattat gaaccgagca aaattgcaag aggactgtca
    29581 ggaaatggtt ttgacgcgat tctagcatta tctcatcgta cttctcatac gacctatggc
    29641 aatccttttt tttcagatgt tattcaatcc atttctgccg tttctgaaca aaataacttc
    29701 gacttgattc tacaaacggc aaaaacacca gaagaagagc tagaaaaatg cgcgaaaaag
    29761 atccaagaaa aaatgatccg aggaattatt attttaagct ctacgatgga tgaaacactt
    29821 ctttttgaat tggatcaata caatattcct attgtggtaa tcggaaaagt cggaaacgac
    29881 tacacgaaca tctactcagt ggacacggat aatttccagg atagttacga tttggtggaa
    29941 acactgatca agaatggaca ccgtcatatc ggctgtctcc atgctccgac agaagtttat
    30001 gtatccaatg atcgtctcaa tggttaccgt caatgcttat ttgatcacca cctagatatc
    30061 cgtaatgaat ggattataga cggcggcttt cgcttagaag atagcatggc agcaatgaat
    30121 gatttaatga agctacgaga aagaccaact gcgatttttt ctaccgatgc attaaaatca
    30181 ttgagtattt acaaagtggc agaacaatct ggaaagcaga tacctgatga tctttcgatc
    30241 gcaggattca gtgatccctt attgtcctcc ctattttcgc cacagttgac tcggatagac
    30301 attccgacaa atcagttagg aatcaaagcc acagaacttt tattccaact aatccaccac
    30361 caataccctg aagaaaaaag aatcattctt tcaaccgaga ttactctcac agattcgatc
    30421 caacaacaag aaatgtagaa cagaattata aaagaacaga aacactgagg ataagtcagt
    30481 tactctgttc ttttttcaat cactcatccc tcgttacccc ctcccggtat tttagcgaat
    30541 ttttcttatt ttccatctgt tttagatagc tgtggataac tataatgttt gtgttaaact
    30601 attttttcca cagggaagaa aaaactattt ttaaccacgt tgtgtataag tttttatagt
    30661 tcgttttttt aaacacattt acccttattt caatccatgt tatttgggga tacttatctc
    30721 taaaacataa atagaataac agacatgtct actggttttg ttttattcga ggaattattc
    30781 aataatagct agcaaaatat taaaaataac cgctctatct tcccatcaat ttattttgca
    30841 ttcaaaaaat gatctatctt tacttttgtt gattgacttc tcttggaaga tgatcgttcc
    30901 gataccttaa ttggatacga accgatgttg ttaagatcct ttcccacttt tcttctagtt
    30961 agacaaatta ttttagac
//
LOCUS       DADVXU010000020        30816 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.021, whole genome shotgun sequence.
ACCESSION   DADVXU010000020 DADVXU010000000
VERSION     DADVXU010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 30816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.021"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(162..335)
                     /locus_tag="KB953_000912"
     CDS             complement(162..335)
                     /locus_tag="KB953_000912"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865907.1"
                     /translation="MNKMKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGM
                     LIYFVFSDDGKYG"
     gene            621..1949
                     /locus_tag="KB953_000913"
     CDS             621..1949
                     /locus_tag="KB953_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HBB6865908.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2151..2984
                     /locus_tag="KB953_000914"
     CDS             2151..2984
                     /locus_tag="KB953_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HBB6865909.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            3000..3737
                     /locus_tag="KB953_000915"
     CDS             3000..3737
                     /locus_tag="KB953_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HBB6865910.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3892..4860)
                     /locus_tag="KB953_000916"
     CDS             complement(3892..4860)
                     /locus_tag="KB953_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBB6865911.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5092..5925
                     /locus_tag="KB953_000917"
     CDS             5092..5925
                     /locus_tag="KB953_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HBB6865912.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5959..6798
                     /locus_tag="KB953_000918"
     CDS             5959..6798
                     /locus_tag="KB953_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBB6865913.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6824..7384
                     /locus_tag="KB953_000919"
     CDS             6824..7384
                     /locus_tag="KB953_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBB6865914.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7583..9304
                     /locus_tag="KB953_000920"
     CDS             7583..9304
                     /locus_tag="KB953_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HBB6865915.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9469..10611
                     /locus_tag="KB953_000921"
     CDS             9469..10611
                     /locus_tag="KB953_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBB6865916.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10627..11838
                     /gene="thiI"
                     /locus_tag="KB953_000922"
     CDS             10627..11838
                     /gene="thiI"
                     /locus_tag="KB953_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBB6865917.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            12411..13058
                     /locus_tag="KB953_000923"
     CDS             12411..13058
                     /locus_tag="KB953_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBB6865918.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKLLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    13187..13403
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13451..16096
                     /locus_tag="KB953_000924"
     CDS             13451..16096
                     /locus_tag="KB953_000924"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBB6865919.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            16406..17719
                     /locus_tag="KB953_000925"
     CDS             16406..17719
                     /locus_tag="KB953_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBB6865920.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            17709..18362
                     /locus_tag="KB953_000926"
     CDS             17709..18362
                     /locus_tag="KB953_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBB6865921.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            18417..19109
                     /gene="radC"
                     /locus_tag="KB953_000927"
     CDS             18417..19109
                     /gene="radC"
                     /locus_tag="KB953_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBB6865922.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            19259..19459
                     /locus_tag="KB953_000928"
     CDS             19259..19459
                     /locus_tag="KB953_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBB6865923.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(19713..21392)
                     /locus_tag="KB953_000929"
     CDS             complement(19713..21392)
                     /locus_tag="KB953_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBB6865924.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(21394..21606)
                     /locus_tag="KB953_000930"
     CDS             complement(21394..21606)
                     /locus_tag="KB953_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HBB6865925.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            21999..23729
                     /gene="efrA"
                     /locus_tag="KB953_000931"
     CDS             21999..23729
                     /gene="efrA"
                     /locus_tag="KB953_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HBB6865926.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            23726..25486
                     /gene="efrB"
                     /locus_tag="KB953_000932"
     CDS             23726..25486
                     /gene="efrB"
                     /locus_tag="KB953_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HBB6865927.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            25581..25778
                     /locus_tag="KB953_000933"
     CDS             25581..25778
                     /locus_tag="KB953_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865928.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            25931..26416
                     /locus_tag="KB953_000934"
     CDS             25931..26416
                     /locus_tag="KB953_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBB6865929.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(26520..27113)
                     /gene="clpP"
                     /locus_tag="KB953_000935"
     CDS             complement(26520..27113)
                     /gene="clpP"
                     /locus_tag="KB953_000935"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBB6865930.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            27293..27820
                     /locus_tag="KB953_000936"
     CDS             27293..27820
                     /locus_tag="KB953_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865931.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            27911..28648
                     /locus_tag="KB953_000937"
     CDS             27911..28648
                     /locus_tag="KB953_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBB6865932.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            28652..29569
                     /gene="miaA"
                     /locus_tag="KB953_000938"
     CDS             28652..29569
                     /gene="miaA"
                     /locus_tag="KB953_000938"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBB6865933.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            29571..30800
                     /gene="hflX"
                     /locus_tag="KB953_000939"
     CDS             29571..30800
                     /gene="hflX"
                     /locus_tag="KB953_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBB6865934.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT        10175 a         5272 c         6576 g         8793 t
ORIGIN      
        1 taggttcgag tcctactgcc ggagtaaata aagcttatgc aaagaaaaag aaatccaatc
       61 acttgggttt ctttttcttt tattattatg atgaatcaag ctttctgttt ttgtcacgta
      121 tgttgtaatc tttacgaaag agtagagttg gctactcata atcaaccata cttcccatca
      181 tcgctaaata cgaaataaat cagcattcct acaccaatca aagcacaagc tgcaccaaga
      241 aaagggatga cattcccaaa gttatctgta ttcataagta tgccaccacc cacaaacaaa
      301 aagccgccag caatcaggtt tttcatttta ttcatctcct ttcttacgtt atagtatttc
      361 cttctttcaa tgagtatttt gaaagctatc ttattgtatg atgttcttct aggcctcttc
      421 tttattatac aggccatttt cgttatctca tagaaatatt cctttttatc tgaaaactat
      481 tgtataccgt atgacatttt tcttcatgaa gttgtaagta tattctcatt caaatgaaaa
      541 tttcatcata gaagagattg tatttctaaa ttcttaagag taaaatacga agtataactt
      601 ggatttggaa ggatgattgg atgaaagcta ttacgcctga tatgacaaca aaatttaaca
      661 aacagtttca agcaaatatc aaacagcagg cattgcagcg ggcagttgtg aagaacggaa
      721 ttactgcttc tgctgaaaat gtttcagcac atgtggagaa tacaccggta ttttctatag
      781 atttggctac ggggaaagtg gctaatcaaa tgcaatcggg gcgttgctgg atgtttgctg
      841 cactgaacac attccgtcat aagatgctga atcaattcca tttaaaagag tttgaattat
      901 cacaaaatta cacttttttc tgggataaat atgaaaaggc aaattatttc tatgaaaaca
      961 tcattgcaac aggaaaagag gcaatcgata gccggaaagt agctttctta ctcgctactc
     1021 ctcaacaaga tggcggccaa tgggatatga tcgtttcatt attccaaaaa tatggcgttg
     1081 tgcctaaatc ggtgatgccg gaaagtgcga atagttcaaa ttctcgagat ttgaataact
     1141 acttgaacaa attactgcgt aaagatgcag tagtgttacg gaaaatggtt gctcaaggag
     1201 aaacattaga cgctattgaa gaaaagaaag aagaaatgct agaaagcatt tataacttct
     1261 tagctatctc attaggaaca cctccaaaag aattcgattt tgagtaccgc gatgaagaaa
     1321 agaactatca tcttgaccga ggactgacac cacaaatttt ttatgataaa tacatcggtg
     1381 taaaattaga tgattatgtt agcgtgatca atgcaccaac aaaagacaaa ccattcaatc
     1441 gttcttatac agtagagatg ctaggaaatg tcgttggcgg gaaagaagtc aaatatctga
     1501 atgttgatat ggaaactttc aaaaaattag cgattgctca gcttgaagaa ggagaatctg
     1561 tttggtttgg ttgtgatgta ggtcaatctt ctactagaac tagtggcatc atggccttag
     1621 atgcttattc aatggatgat ctatttgata cggattttac aatgactaaa gctgaacgtt
     1681 tagattttgg agaaagtctg atgacccatg cgatggtatt gaccggtgtt gacttagtag
     1741 acgggcaatc aacaaaatgg aaagtcgaaa atagctgggg cgaaaaagtt ggaaagaatg
     1801 gcttctttgt tatgagtgat gcctggatgg acgaatatac gtaccaaatc gttgtaagaa
     1861 aagatttatt gacgaaagag caattaaatg cgttcaatga agaaccaatt gttctatctc
     1921 cgtgggatcc aatgggtgct ttagcataga cgagtatttc aatgaagagt atgtagtaaa
     1981 gataaaaaga gctggcttaa gccagctctt tttatcttta aagtccattc cctgtttttt
     2041 atggtccctg tgctataatt ctaaataaag gataaggatt ggaaataatc cttttatcat
     2101 taaaaatatg atgacgaagt agaataaaat tgggagtagg ggggaaagtc atggatatcc
     2161 aaactgtttt atctgatctg aaattaaatg gtaaagtgat tccagttgtt ggtggcgatg
     2221 ttaatcagac ctatcgtata aaaaccgaac atcgtgctta ttttttaaaa atccatccga
     2281 atgtaaaaaa aggatttttt gaagcagaag tagacggatt aaaagaactg tcagcattcg
     2341 tacgtgtacc ggatacgtat atgctaggtg aaacaagtga aggcgcttat ttgttaatgg
     2401 aatggatcga gccgggaaaa ggggatcaac gagatcttgc tgcagcttta gctaatctcc
     2461 atcagcaaac agcgccacaa tttggttttc gtaaagataa ctatttgggt acacttgttc
     2521 agaaaaattc ttttgaagaa gattggtgga cttttttctt taaagatcgg cttgaatccc
     2581 agatttcttt ggcagaagaa acgaatcgat ggaatgtaca gcgtcaagaa aagtatctgc
     2641 gatttaaaga acgtgtattg aaatcggttg aaccaaaaaa gatcactcca cgcctcttgc
     2701 acggagacct gtggagtggt aatgtgttct ttgaccagca agggcatcca gtttttgtcg
     2761 acccagctgt ttcttatggg aatcgggaac aagatattgc aatgagccag ttattcggtg
     2821 gtttccgacc ggaattttta gatgcttatc aaactatatt tccattagaa aagggatgga
     2881 aagaccgact gccgatttac cagttatatt atcttttggc acatttgaat atgtttggag
     2941 aaagttacgg ttcccaagta gatcaattac tagaaaattt ttaagaggag ggcgtgtttt
     3001 tggcaaaaaa atataccaaa aagcatgaag tttcatatta tgagtgtgac atcaaccaaa
     3061 cgatgacatt tccttcgatg ttaggagtag ttatcaaaac gtctgaagat caaagcgacc
     3121 agttgggaag aggcagtgaa ttcgtcaata gctttggact tacttgggtc atcacgaatt
     3181 attccatgga gattaatcgt ctccctagag tcggtgaagt aatctcagta acgactcaag
     3241 ccatggagta caataagttt ttctgttatc ggaacttctg gatccatgat gaagatggga
     3301 aagaattagt caaaatagaa tctgttttcg tattgatgga ttttgtgaat cgaaaaatga
     3361 gcagtgttaa tgaagagatc attgctcctt ttgagagtga gaagatcaag aaaatcaagc
     3421 gtcaggaaaa aattgaaaag attgattcgg gcttcatgtt gccatatcgt gttcgttttt
     3481 atgatatcga tagtaatcag catgttaata atgcgatgta ttttaattgg atcattgacg
     3541 tattaggata tgactttttg actacgcatg ttccagaaaa agtaattatt cgatttgata
     3601 aagaagtaga gtacgggaat gagatcgaaa gccattttga acaaatccct atcgaaaatg
     3661 gtgtcaaaac aaaacatgaa atccgaatgc aagaacagct gtattgtgaa gcaaatatta
     3721 tatggaaaaa taaataaaaa agaggttgtg acagaagggg gcaacttcaa gaaataagaa
     3781 gaaatttacg aaaattgttt ctcaaatttt tgtaaatttt cgacttagtt ttcgaagaag
     3841 ctatttttgt cacagcctct ctttttaagt ttagctatta gataatgatg tttagttttg
     3901 gataaacgaa atcaattctt cacccgacaa accatagttt acagcaatca atagttttaa
     3961 acgtgctttt tgactattaa tactgttaca gaaaataatc cccatttttt ctagttcttt
     4021 tcccccgcct tcataatcat aaacaggctc tgcgattcca ttgaaacaac gagaaacaag
     4081 tactacaggg attttttgga caagaagctt ttccaaagca gctaaggctt gtggagggag
     4141 attccccgca cctaaagctt cgattaccaa accgtctact ttcgtatggg cgatggcttc
     4201 caataagtcc ccttgcatcc cagcatatgc tttaacgatc gggatagtcc catctacagc
     4261 ggaaatgtcc agtcgctttg tttctaaaag ttcctgcaag aataaaattc gattcttagt
     4321 tactaagccg atgggaccca atgtaggtgt tcggaaagta gcaacatttg tggtatgggt
     4381 ctttgtcacg tagcgtgcag aatggatctc atcgttcatg accaccaaaa cccccttatc
     4441 tagcgcttct tcacaacttg cgacacgaat cgcactttca aaattataaa ggccgtcact
     4501 tccaagttca ttacttgaac gcattgcccc agtcaaaaca attggtaatt tgttgccaat
     4561 cgttgtatca agaaaaaaag cagtttcttc taatgtatct gtaccgtgtg tgatcaccac
     4621 tccggatata cctgatgcat aagcggacaa gatacgttct tttaatacca acatgtgaga
     4681 aggcatgatg tgtggtgagg gaagattgaa gatatcttca actaataatt ctacatgctt
     4741 ggggattgtg atttctgcat ctaataatgg gtttgccaca tcaggactaa caccgcccgt
     4801 gagcatgttt tctttcatgg cgatcgttcc gccggtgtgt agtacaagta tttttttcat
     4861 tttacagact tccttacgaa taattttgca tagccgttaa atcttattgt atactatata
     4921 aaagagtttt gctatcagtg aagaataacg aatagcctaa cttatcataa aaaagctgac
     4981 cttattaaaa tggaaaagac tcaaccttga cctcctaata caatggagta tctggttgag
     5041 tttcttttac aagaaagcag ccaaggaaat caatcacgaa ggtggccgga aatgatcaga
     5101 atcggattaa catcgtttaa agaacatgac aaattaactg gtaaaaagaa taacactctt
     5161 tatgaatatg cagggtttct acccttagtg gaattagata ctgcctatta tggaattccc
     5221 cgaaagtcct cagttgagaa ttggataagt gaagtacctg aatcatttcg ctttgtagtc
     5281 aaagtctatc aaggaatcag cggtcaagga gactggaagc cttattatca gaccgaagaa
     5341 gagatgattg cgcatttttt agatgttatg tcgccaatgg tagatgctgg aaagatgttt
     5401 tgctttttag tccaattccc ctcatcattc aaatgtacga aagaaaatgt ccagtacttg
     5461 cgcaaactta gaaagtggtt tgagggttat cctgtttcta tagaattgcg ggattattct
     5521 tggtacagcg atgaatttct tgaacgtaca agaaacttta tgtctagagc cgctttttct
     5581 ttagccatca tcgatgagcc acagttgctg aatacaacga ttccttttga tccatttgtc
     5641 accaatccga actttgctct ttttcgattc catggtagga atgcgcaagg atggcaagca
     5701 aatgataaag agtggcgtaa aaaaagaacg ttatatcgtt attcagaaaa agaactgcaa
     5761 gaactagcac atgaagtcaa agtagttgat tctaagacaa aagaggtagg tgtgattttt
     5821 aataataatt ctggtggtga tgcggctgaa aatgcattag cactgcaaaa aatattaaat
     5881 atcaagccag atggactcaa tccaacccaa atggatctgt tttaagggct tattttattc
     5941 aagaaagggg cgaagtctat gatcaaagct attttttttg atattgacgg aacattggtc
     6001 accaatcgtt caaaagcgtt ggaatcaaca aaacaagcaa ttgaatatgc tcggaaaaac
     6061 ggcattttat gcggtgtagc aacggggcgc agcccagtaa agatcaaaga aatcattgat
     6121 gaattggaat tagatatgta tgtcgtgtat aatggtcaac tggtttttac tgcagatcgc
     6181 acgattatcg accacccttt tgaacaaaaa gtattggaac atattgttga atttgcagat
     6241 gagaaccatc gacaaatcgt ttttggtgcg agaaatcggt tagatggatc aacgacgatg
     6301 ctattaggac aatcgatttt tatcaagcga ctagtcagct ttttaccgcg taaattcccg
     6361 gtacgtctga tgaaaaaaat cctccaggtt tttagccctc accgacaaaa ggatcgttat
     6421 gaaacgttag agatattaaa agaaccaatt tatcaatgta tattgctcag tccggagtca
     6481 gaacagaaaa agttagaaca tcgctttccc gagtgtacgt ttcagcgttc aaatacgtat
     6541 actgtagata tcatcccaaa aggtggttca aaattactag gcatccaagc tttcgcaaat
     6601 gctgtcgaaa ttgaaatgga agaaattatg gcattcggcg atcattacaa tgatatcgag
     6661 atgctgaaag gtgtaggaat tggagtggcc atggggaatg ctcagatcga agtaaaacat
     6721 gctgcagact ttgtgactca atcaaatgaa gaagatggga tatacgaagg actgaaacac
     6781 ttcggtctga tttattagtc aatgaaagaa ggaaaaaatc agtatggaag cagagacacc
     6841 ttttcaaaaa gtagaaacat tccacaaaac ttttgatcca agaagaccag agatacccac
     6901 ggcttttaca gaaaatgaag ctcatacacg tgctgcgttc aaaatagaag aacttgttga
     6961 atttgttgca gctacttcac ctaataaaga acaactgcat gaagctgtta tgctgctgca
     7021 cgaagcatta gataaagcag aagaaaaaat acttggtaaa acacagtggg gaagtgatcc
     7081 gctggttgaa caagtagatg cactgacaga tatcctttac tttacttatg gttctttttc
     7141 attactagga atagtgcctg atcgaatatt cgaaatcgtc catcaggcaa atatggggaa
     7201 attatttcca gatggaaaac ctcattatga ccctgtaaca aataaagtgt tgaaacctga
     7261 aaactggcag atggaatatg ctccagaacc taaaatcgcg gcagaaatcc aacgtcagat
     7321 tcaagaagcg attgctaaga agaaatccgc tgatataggc atgtgcacaa atgaaaaaga
     7381 gtgatcagag tttcactatt tttgttatct ttatacgatg gaaaaacaaa agattttcgt
     7441 ttaagttagt gaatgcatga aaatagtatg ctataatcta gaatagatgt cttttgcacg
     7501 agagacgtat tatttggatt tatgccaagc agaacgccat ctgttgcata aaagatgcat
     7561 actgaaaacg gaggaaccat aaatgggaaa taatgttatc atcggaatcg tcattgcaat
     7621 cattatagtc gccattgtcg tctttttggt tagttacttg atgaagaaaa aaaaccaaga
     7681 tcgattggat gccttagaaa aacgcaagga agaattattt gacttgccag tgattgaaga
     7741 agtcgatgat gttaaaaaaa tgcatctggt tgggcaaagc caaaatacct ttcgtgaatg
     7801 gagtcaaaaa tggaatgaca tatctacttc ctcgttcgct gaactagaaa gtcaaatttt
     7861 cgaagtggaa aacctcaatg aaagttttcg gttcttaaaa ggaaaaaaag cagttgttga
     7921 agcagaagct acaatggacc aaatggagaa acaagtgacg gaaatccgtg aaggtctaaa
     7981 ggaattgcgg gaaacggaag aacgcaactc actagctgtt cagcaggcac ttgacgtcta
     8041 tgaagaactg aaagaaaaag tcagagaaga cgagcatgaa tacggaccag ctctttttga
     8101 attgcaaaaa cagatcaaag tgatcgaaaa agaatttacg cagtttgtga ctttaaacac
     8161 ttctggagac cctgttgaag cccgcgaggt tttggataaa gcagaaaaac atacgtatga
     8221 agtggaagct ctgatgaaaa aaattccacc gctgtatgag gagctccata ccactttccc
     8281 tgaacaactt gaagaaatca gtgatactta cgagaaattg atggaagaac aatatgtatt
     8341 cccagaagaa gatcttgctg aagatatcgc aaaagtaagt cgaagaattg aaaattcatt
     8401 agccaatctt gaaaaaacag aagttgaaac ggtcgaattt gaaaaccgtg aaacagctga
     8461 tttgattgat tcgttatatg atatcttaga acgggaaatg gaagctcaga gatatgtaaa
     8521 aactaaccaa tcaacgattg cagagtatat caaacataca acaaagaata accgtcaatt
     8581 attgattgag ctggatcaca catctcaaag ctatacattg aaccacaatg aaattggccg
     8641 tgtacgaggt ttccaaaccg aagtggaaga aatggaacgt caaaatgaac agatgattcc
     8701 acagatccgt caacacgaaa ttccttattc agaagtgcgc acgttctata aaacagtctt
     8761 caaagtctta gaagatatcg aaaagcaaca agtagagatc gacgactcat tacatgaatt
     8821 gagaaaaggc gaaaaagaag cgcaagaaaa gatcgataca ttcgaattca aattgcgcag
     8881 tttgaaacgt tttgtagaaa aacaacgttt gcctggcttg ccgaatgatt acctagaatt
     8941 cttttttgtt gctactgacc gtattgaaga actaagtgtt gtcttgaata aaattcgagt
     9001 aaacatggaa gaggtcaatc gtttagttgc tctatgtgaa gaagatctag aattattaga
     9061 taagaagaca catgatttag tagatgcagc agcattaacg gagcaaatgc tccaatatgc
     9121 aaatcgttat cgtcatacac acgaagaagt gagagaagca attgataaaa gctactattt
     9181 attcaataaa gaatatcact atcaagaagc attagacgaa atcggtacag cattagagcg
     9241 agtagaacct ggtgctttca aacgcatcga agatttttat tttaatcatc cagacttagt
     9301 ataaagcaaa aaagttagta aaccagcaag agcaagacgg attattgtct tgttcttgtc
     9361 tttttttgta tatttgaaaa ttgaaacaag tgaaaatttc acctgctctt gaaagcacct
     9421 tggacaaccg ttataataga aaagaatttc aaaaaagagg ggtttcttat gatatatttt
     9481 gataacagtg caacaacccc aatctttccg caaagcttag atgcatatgt aaaaaccagt
     9541 cagcggatta ttgggaatcc atcaagcttg catgatttag gcaatcaagc taaccggttg
     9601 ttgcagcaag caagaaagca gatcgctgat ttgatccatg tagaaccaga agaaatctat
     9661 tttacttcag gcggaacaga aggagataac tgggtattaa aaggaacgat gatcgaaaaa
     9721 agagaatatg ggaatcatat gatcatttca ggagtcgaac atccggccgt cagcgagaca
     9781 gcagaacaat taaaagaatt aggaatcgaa gtaagtatcg ccccagtgga taaacgcgga
     9841 tttgtacgtg tcgacgaatt gaaagaatta ataagaaaag agacagtatt agtctctgtt
     9901 atggctgtga ataatgaagt aggatcgatt cagcccatcc aggaaatcag tgatcttttg
     9961 gatgattttc ctaaaattca tttccatgta gatgctgttc aggcgattgg aaaagtacca
    10021 ttctcgcaat ggttgacaga aagagttgat tttgcaacat tttcagctca taaattccat
    10081 ggcccacggg gaactggttt catttattgg aaaaagggtc gtcgtttagc accattactg
    10141 acaggtggag ggcaagaaag aaatcagcga agtgggacag aaaatgttcc aggtattgtg
    10201 gctatggtta aagctttacg tttatcatta gagataaagg aacaaaaacc tgagcagaca
    10261 caacgcttaa aagtagctct aatcaatgcg ttgaagacgt atgagaaagt gacgattttt
    10321 tcagaaggaa ctgactatgc accgcatatt ttatgttttg ctctaaaagg aattcgcggc
    10381 gaagttctcg tccatgcctt agaagaaaaa caaatatttg tttctactac tagtgcctgc
    10441 tcctcacgta aacatatggc aagcagtaca ttgcacgcaa tgcatgtacc aaatgatctg
    10501 gctacatctg ctgttcgaat cagcttagat gaaaacaatt cgcttgcaga agtcgagcaa
    10561 tttatgatta ttttcaagca gctttataag aaattttctg caatcaatta aaagaggagt
    10621 gttcttgtga aatacagtga aattatggtc cgttatgggg aactttcgac aaaaggaaag
    10681 aatcgaaaaa gctttatcat gcaactagca caaaatgtca gacaagcttt gaaagacttt
    10741 ccagaaatca aaatccatgc tgatcgggac cggatgcatc ttttattgaa tggcgcagac
    10801 gggcagctag ttattccaaa actagcaaaa atatttggga tccaaaattt ctctccaagt
    10861 attcgtgtag aaaaagatgt cgaagttttg aaacaagctg ttcaagacat catgaaagaa
    10921 atttatactg ggaaagaaac atttaaaatt atcgctaaaa gaagcgatca ccaatttgaa
    10981 ttgacaagta atgaattgaa tcaaacatta ggtaatgctg tttttgatat tttcccgcat
    11041 atccaagttc aaatgcgtca accagatatt ccattacgtg tagagattcg gcgtgatggc
    11101 gcttatctat cgtatgaaac gatcaaggga gctggaggat tgcccgtagg aacaagcgga
    11161 aaaggaatgc ttatgctttc tggggggatt gattctcctg tagcagggta ctttgccatg
    11221 aagcggggag tggaaattga agctgttcat tttgctagtc cgccgtatac aagtgaacaa
    11281 gcactgcaaa aagcaaaaga tctaacagcc aaactagcac cttatgtggg aacgatccat
    11341 tttatcgaag tgccatttac cgagattcaa gaagaaatca aaaagtcagt gccacaagga
    11401 tattggatga caattactcg tcgaatgatg ttgcgtttga ccgatgaaat tcgccgtatt
    11461 cgtcatggtt tagtcatttt gaatggtgaa tcattaggtc aagtggcttc acaaactttg
    11521 caaagtatgg tggctatcaa tgaagtaacg aatacgccaa tcattcggcc tgtagcgaca
    11581 atggataaac ttgaaatcat tgaagtagca gaacagatcg atacatttaa tctttctatc
    11641 cagccttttg aagattgctg tacgattttt gctcctccac agccaaaaac acgccctcaa
    11701 ctggaaaagg tccatcaata tgaagagcgt ttagcaatag atgaaatgat ccaacgagca
    11761 ctagctggct tgaagatgga agagatacaa gcagaatcgt ctaatgaaaa aactgaagaa
    11821 ttttcagaat tgctataaga ggttatgaaa atagtgtttt gaccagagta ttagagaaac
    11881 aaaacgaaaa aatagctcca tgtattttac cgtttttgaa ctaatgtcga agattagctg
    11941 attgaatacc atttatcaag aagttatgga aaagccggtt cttttttact aactgaaaaa
    12001 taaaatttta tttagagatc aagctacagt tgataaatga tataaaaaag agaatttccc
    12061 atgaaatagc gaggttgtga cagaagtggg cagcttcaag aaataagaag gaatttacga
    12121 aaattgcttt tcaaattttt gtgaaatttc ggcttatttt tcgaagaagt tacttctgga
    12181 acaccgtgta tcagaggttg cgaaaaagct gccctgcatc aagtaataag aacagccaaa
    12241 caaaaatagt tcctcatatt ttacgtttgg ttgagcttat taccgcagat gcaagctttt
    12301 gaacaccgtt ttatttgtct cacacccttt tccttcctac ttgtaacctt gctcccgtgt
    12361 tagaaacatg ttacactaaa tatgtgaaat tattaacaag gagcgtcgtt atgcgagaaa
    12421 aaacagatac actgccaaaa gcaactgcga aaagattacc tttatattta cgctacttga
    12481 agttgctgga tgactcaggt ataagtagaa tcaaatcaaa tgagttcagc gaaatcaccc
    12541 aaattccttc tgcaacgatc cgcagagact tctcgcaatt aggcgagcta ggcagaagcg
    12601 gatatggcta tgatgtccct tttcttatcg atgtattcaa taatatcctt aatactaaag
    12661 aagaaaaacg aatcgcatta gtaggatatg gaaatcttgg caaagcattg aaacacaaca
    12721 attttagaag aaatgaaaat ctaaatatcg tgtgcgtctt tgataatgat ccagcactga
    12781 tcaatcgagt aattgatgga gaaatgatct atccgattga tcgtttcgcg gagatagcca
    12841 aagcaaaaaa tgtgacagta gcaatctcta cggtaccaag taaatattca caatctgcta
    12901 ttgatgaaat tgtcaaagga aatgtaaccg ctatacttaa ttttgcgcct gacagagtaa
    12961 ctgtcccagc ttatgtcaat gttcaataca ttgatttgac aaccgagttg cagacgctta
    13021 tttactttga tgagaattac tcagaagttt tttcttagga aaatttgagt aggcttattg
    13081 atcttgcgag aactcgtcgt aattctttca aaaatttttt agtactaaat gaagtggttt
    13141 tttcacgacc acttgttgac taaaaaaaaa aaccattgta gaatgaagca taacgctgaa
    13201 caagaggagt aactggcaca ttcattctta gagagagaac tatctgctga aaggttctca
    13261 atgacaacca gtgaaggtag cttgggagtt tctgtcttga atggagtaga gatagacgag
    13321 tcgtgatcgt taactcactt gagaggcaat aatgattgat tattgcaatt taggtggtac
    13381 cgcgtaaatc atacgtccta gaatacgaaa gtattttagg acgtttttta gtttaaagga
    13441 ggaaaacagc atgacggaag aaaataacct atcaacaaaa tataatccgc aagaagttga
    13501 agctggacgt tatcagcatt ggttagaaca agatttgttt aaaccgagcg gagataaaaa
    13561 agcaaaacct tattcaatcg taatcccgcc cccaaatgtt actggaaaac ttcatttagg
    13621 gcatgcttgg gatacgacct tgcaagatat gctgattcgt caaaagagaa tgcaaggata
    13681 tgatacttta tggctgcctg gtatggacca tgcaggaatc gcgacacaag caaaagtaga
    13741 agcgaagctt agagagcaag ggatttctcg ttatgatcta ggtcgtgaaa agttcatcga
    13801 tcaagtgtgg gaatggaaag aagagtatgc gagccacatc cgcgaacagt gggctaaaat
    13861 gggcttatct ttagattaca gtagggaacg ttttacatta gatgatggat tatctgaagc
    13921 agtacgtaaa gtttttgtta ccttatacga aaaaggtttg atttatcgtg gtgaatatat
    13981 catcaactgg gatcctcaag cacgaacagc tctatctgat atcgaagtta ttcataaaga
    14041 tatcgaaggc gccttttatc atatgagcta tgaactagca gatggatcag gcgtagtaga
    14101 aattgcgaca acccgtccag aaacgatgtt gggagatacg gcgattgctg ttcatccaga
    14161 agacgagcgt tatcaagcgc tcatcggcaa aaaagtgatt ctacctttag tcaataaaca
    14221 aatcccaatc attgcagatg aatatgtaga tatggaattc gggacaggtg tagtaaaaat
    14281 cactccagca catgatccaa acgactttga ggtaggtaat cgtcatgatc ttccacgcgt
    14341 aaatgttatg gatgatgatg gtacgatgaa tgaactagca ggaaaatatg ctggaatgga
    14401 acgttttgct gctcgtaaag caatcgtgca agatctgaaa gaaatcggtc gattgattaa
    14461 aatcgatacg atgatccaca gtgtgggtca ttccgagcgg acaggtgtcg ttgttgaacc
    14521 tcggctgtct actcaatggt ttgtaaaaat ggcgccgtta gcgaaaaaag ccattgataa
    14581 ccaagaaaca gatcaagcag tggaattctt ccctccaaga ttcaatcaaa cgtttttacg
    14641 ttggatggaa aatgtccatg attgggtaat ctcacgacaa ttatggtggg gacatcaaat
    14701 tcctgcatgg tatcacaaag aaacaggtga aatgtatgta gggatggaag caccagcgga
    14761 tagtgaaaac tgggtacaag atcctgatgt tttagacact tggtttagtt cagctttgtg
    14821 gcctttctca acaatgggct ggccagatac ggaaagcgaa gattataagc gatacttccc
    14881 tactagcact ttagtaaccg gttatgacat cattttcttc tgggtaagca gaatgatttt
    14941 ccaaagcttg gaatttactg gcgaacgtcc atttgagaat gtcttgattc atggattgat
    15001 tcgtgacgaa caagggcgga agatgagtaa atcattaggt aatggaatcg atccgatgga
    15061 agtaatcgaa aagtatggag cagatgctct ccgctggttc ttatcgaatg gttctgcacc
    15121 aggacaagat gtccgcttca gctatgaaaa aatggatgct tcttggaact ttatcaacaa
    15181 aatctggaat gcttcacgtt tcgttatcat gaatatcgaa ggaatgaccg tagaagagat
    15241 cgatttatct ggagaaaaat cagtggctga ccggtggatc cttactcgtt tgaatgaaac
    15301 gatcgaacga gtaactgagc tatttgatcg ttttgaattt ggtgaagcag gtcgacaact
    15361 gtataacttt atctgggatg atttttgtga ctggtacatc gaaatgagta aagaagtact
    15421 ttatggagat gatgctgttt caaaacaaac cacccgtagc atcttagtcc atacacttga
    15481 tcagatccta cgtttattgc atccaatcat gccattcgtt acagaagaaa tatgggaaca
    15541 tattcctcat cagggaaatt ctctagtagt ggcagaatat cctgttgtac gtccagagtt
    15601 tgatgatgaa acagcttcta aagggatgga agtattgaaa gaattgattc gagccgttcg
    15661 aaatattcgt tcagaagtga atacaccatt gtctaaacca attacgttgc taattaaaac
    15721 aaacgatgct aaaatcgatc aatttttagt tgaaaataca agctatatcg aacgcttctg
    15781 taatcccgaa gagttgacga tatcaagtga gatcactgcg ccagatctag cgatgtcagc
    15841 tgttctgaca ggtgcagaaa tcttcttgcc gttagcagga ttgatcaata ttgaagaaga
    15901 aatcaaacga ttagaaaaag aactggaaaa gtggacaagc gaagtaaaac gtgtccaagg
    15961 aaaactttct aatgaacgtt ttgtttctaa cgcgccggaa gaagtagtag aggcagagcg
    16021 tgcaaaagaa aaagattatt tagagaagca aacggctgtc aaagaacgaa tcgaaagttt
    16081 gcgtactatt caataagaat agaaaaagag agtgtgaaat cagctagcta ttactaaaag
    16141 ctagctgatt gaacatcgtt acaggaaaga gtttgagcct cactcttagc ttcgattcat
    16201 aaggagatgt gacataaatt ttgtcgcatc tcttttttag tctactctta ttatgtagga
    16261 gaaggaggga ggtggatcat gtatcaagag tcaataaagt agttgataaa atcgttcctc
    16321 tctctgtttc ttttctcttt gtcatacttt tatttttctt atgtgttcta tttgttataa
    16381 taaaagagaa atgggggaat tgttgatgac gattgaagaa gcaatagaat ggatccatag
    16441 tcgattacca tttggttcac gtcctggcct tgaccgagtc gaagctttat tagaagtggt
    16501 agggcatcca gagagaaaag taaaaacgat ccatatcgct ggaacaaacg gaaaaggttc
    16561 tactgtagct tatttgagaa gcctattaga agaggctggg ctgacagtag gtacatttac
    16621 ttcaccttat atcgaaacgt tcaatgaacg gatcgcaatc aatggcaaaa gcattccaga
    16681 caatgatttg atccagcttg taaaaaaata tcagccactg atcaaacaac ttgatcaatt
    16741 tgaagcagta gctggtatta ctgaattcga gacattaaca gcaatggcat ttgattattt
    16801 tttgatgaag aaagtggacg tagcgattat cgaagttggc ttaggcggct tattggattc
    16861 taccaatgta gctgtaccaa tgctgacggg aataacaacg attggacttg atcataccga
    16921 aatacttgga gatacaattg aagaaatcgc tgcacaaaaa gctggaatca tcaaacaagg
    16981 cgttccagta gtaacaggaa atattgcccc agaagctttt actgtcatcc aacaaacagc
    17041 taaagcaaaa aatgcaccgc tttatgtata cggggaaaat tataaaataa actatctgca
    17101 tcctgatgag atgtggggag aagtatttga attttttaat gaaataggaa agatccctca
    17161 attaaccact ccgcttttag ggcaacatca agctgaaaat gcaggagtag ccatcgaatt
    17221 gtattttgtt tactgtcaat tggaaaagct gcctttccga gaaaaagatg ttttgaaggg
    17281 attgaagaaa tctcgatggc caggaagaat ggagcgtttg agtaacgagc cattagttgt
    17341 tttagatggt gcccataata cgcatgcaat gaaccggctt gttgagaatt tgaaaaaaga
    17401 attcaaagga tatcaaatca acattttgtt ttccgcctta cgaactaaaa atgttagaga
    17461 aatgctagaa cgattgcagc aagtaccaaa tgtccatatt tatttgacga cctttgaaca
    17521 tccaaaagcc atcgaattaa gtgaagtatc agatttagca agtgagagag tatcaatcgt
    17581 ttcattgtgg caattcggat tagcagatat attggaaaag atgacaaatg aagacgtatt
    17641 agttgtgacc ggttcacttt atttcatttc agaagttcgc catttattaa tacaattagg
    17701 aggagatcat gaagtataat gcagtgattt ttgacatgga tggtttgctg ttcgacacag
    17761 agttagtgta ttacgaagcc acacaagtag tagcagacaa gatgggtttt ccttatgata
    17821 aagacctgta tttaaaattc ttaggtgttt cagatgaaga agtatgggcg aattatcata
    17881 tgattttctc ggaatttggc aaaacaagtg tacaaagatt catagatgat tcataccagg
    17941 aaacgcttga acgtttttcg caaggagcgg tacaaatgaa gcccggtgtg ttggaactcc
    18001 tctctttttt agaagaacat cagattccca aagttgtcgc ttccagtaac caacgagcag
    18061 tcattgaact tttattgaag aagaacaatc tgtttgaacg atttgagacg attgtttctg
    18121 cagaaaatgt tcaacgagca aaaccagacc cagaaatttt cttgttagct aatcagaagc
    18181 taggtacagc gaagaaagaa acattgattt tagaagattc acaaaatggt attttagcag
    18241 cttctgcggc tgagatcccg gtagtcatgg taccagatct gcttccacca tcccaagaat
    18301 tgatcaaaaa aacagctggg gttgtttctt ctttacatga aatccctgca ttgttaaaat
    18361 aaaaatccat ctcttttcgt atcttatttt agataagata gaaaggagat gtttttttgg
    18421 gaaaacgatt gatcaaagaa gtacctacta gctctttacc aagggaacgt atggaaattt
    18481 atggcgcgga agctttatcg gatcaggagc ttttggcaat cttactgcgt accggtcaac
    18541 acccttatag tgtgatgtcg attgctggaa atctgttaaa aacgtttgga ggactggcct
    18601 ctttacgaca ggctactttg catgagttag aagagattcg agggatagga cgcgtgaaag
    18661 caatcgaaat caaagccttg atcgaattag gtcgacgaat tcagacggat catgaaagag
    18721 cggctccacc ggttcgtacc agctacgaat tggcccagca attgattctg gaaatgaaag
    18781 atcacaaaca agaacatttg gtttgtattt atttggacac gaagaatcaa gtgatcttaa
    18841 aaaaaacagt gttcatcggt tcgctgaatc agtcagtcgc tcatcctaga gaaattttcc
    18901 attatgctgt acgttattgt gcagcaagga tcgttttagc gcataatcat ccttctggta
    18961 atgttattcc atcgcagcag gatatgaatt ttaccaaaag gatacaaaag tgtggagaaa
    19021 tgatggggat cactgtactg gaccacttga tcatcggacg caaacgatat tttagtttga
    19081 gagaagaagg aatgatggaa gagaagtaag ttgctttctt gcaaaaaaaa tgaagcaagt
    19141 gtacaattat attgtaattt tgtttgggaa tagaagttga aacatctgtt gcacctctat
    19201 cgtacacgag caatgttaca atttaaatta gtgcttttgg cacgaggagg atattattat
    19261 ggaaaacgga acagtaaaat ggttcaacgc tgaaaaagga tttggattta tctcacgtga
    19321 agatggaagc gacgtattcg ttcacttctc agctatccaa ggtgacggat tcaaaacatt
    19381 agaagaaggt caagcagtga ctttcgacgt tgaagaatca gaccgcggcc cacaagctgc
    19441 aaacgttgta aaagcttaat tttatctgta aatcaaattg atggtagaca tcaattttta
    19501 cgagagaaca aagcgagtaa caaaaatacc tgccacaaga gacaatttct cttgtggcag
    19561 gtattttttt gtatgaaaaa gtaaataaat ttaagaggct atgaaagagc caccttgtat
    19621 caaggaataa gaacaaacaa aaatggcttc tcatgttttt catttgtttt ctcttatatt
    19681 caggacaagg tgctgatttc ataacctctt atttattctt catctggtgt cattaccatt
    19741 ggcaaaatca ttggatgtct ttctgtacgt tcgaataaga aaggctgaag cgcgccagtc
    19801 atcgcttctt tcaatttata ttctgaacag tcaggtccat tcaaacattc tcttaaagcg
    19861 tggaataaga ttcgctgagc ttcattgatc atttctcctg attctcgcat gtagacaaat
    19921 ccacgagaga gaatatctgg accagccaag atttcttttt tcttgatatc cactgtcgcg
    19981 acagctaata caagaccttc ttctgaaagc aggtgacggt cacgtaaaac gacattacca
    20041 atatcaccga tcccgctacc atcgatatac acatctccag ccacaaaatg cccagcagga
    20101 cgcgcagaat cgctagttaa cgctaaaaca tcgccatttt ccatgatgaa gcagttttct
    20161 gcaggaacac ctacatcttg tgctagagaa gcatgtactt tcaacatgcg gaattctccg
    20221 tggacaggaa taaaatattt tggcttcatc aaacgaagca ttaatttttg ttcttcttgt
    20281 ccaccgtgac cggaagtatg gatgttattg atttttccgt gaatcacttc tgcaccagct
    20341 tcagacagca aattgatcaa atgattgaca cttgttgtat taccaggaat aggtgaactt
    20401 gaaaagatca ctgtatcgtc tggctggatt tggatttgac ggtgagtacc attcgcgatg
    20461 cggctcaacg cggccatcgc ttctccttga gaacctgtac ataggatcat cgtttcattg
    20521 gcaggcaagc gattgatttc gtgtgcatct acaaatgtgc cgtctggaac tttgatatat
    20581 cctaaacgtt gaccgttgac gatggcattt tccatgcttc gtccgaaaac agcgatttta
    20641 cgaccagttg ccacagcggc atctgctgct tgctgcaaac ggaagatgtt agaagcaaag
    20701 ctggcaaaaa tgatacgtcc ttcgattttt gaaaagatct tcaaaataga atgcccaatc
    20761 gttttttctg atttagtaaa tgttggaatc tcagcatttg tactatcaga taaaaggcac
    20821 aagactcctt cttccccgat acgagccatt ttatgcaagt ttgctggttc gccaacagga
    20881 gtgaaatcga atttgaagtc tccagtcgct acgatatttc cagaaggtgt tttcaccacg
    20941 atccctaatg catctgggat actatgggtc gttctgaaaa agctgacaga agtctttcta
    21001 aaacggatga tcgtatcttc attgatctca tggagctctg catctcgaag caagccatgc
    21061 tcatctaatt tgtttgtgat caacgctaat gcgagaggac cagcatagat aggaatattt
    21121 gcttgtttca gcaagtaggg tattccgccg atgtggtctt cgtgcccgtg agtaatcacc
    21181 aaggctttta ctttgtgtaa gttttgtatg atgtagctgt agtcgggaat gacatagtca
    21241 attccaagta aatcgtcttc cgggaatttg atcccggcat caatcaaaat gatttcatct
    21301 tggaattgta ccccatacgt atttttcccg atttcaccta atccaccaat agcaaagacg
    21361 cctgtctcgt tatttttgat ggaaagtttc atattaaaac tccactaatg agaaatcagc
    21421 attttctttt tcgtactcta gatgacttcc ttctagaagt tgaacgaatt caatattgta
    21481 tggggtattt tcttccacac gttggcgagc gtctacatcg ctttttgcct caatgtaaag
    21541 agattttgtt tcttcccgtt tagggttgcg tacttttgtt tcttgataat aaactttata
    21601 gatcattgtt ttgtctcctt tatctgcgta aagcagtgat ttagctagtt gattttttta
    21661 gacaacacaa aacgagggga caagccacct ggtttttcat atgctttgac tagaatactg
    21721 gattcgagtc aaagagacaa gcctcgtttt tgcttatctg ttcggatatt ttttcaaccg
    21781 taccttctgt ataaaaaata tacaacaatt aattgttatt ctagcataaa acagcaaata
    21841 agtatagaaa tcaactagat tgaaagaaaa aatcaatgtt tatcatcccg ctataatcat
    21901 aatgactttt ttatgagaga agtgttctat taataggctc gttgtgctat catttttagt
    21961 aatgaaaatg taacaaaaga ttaaaaggag atgagaaagt gaaactaatg tggcattata
    22021 ccatgcgtta taagaaattc ttgtttttca atttcatatg cgtatttggt tttattttga
    22081 ttgaattagg acttcctacg attttagcac gtatgatcga tgtaggaatc cgcaatgatg
    22141 attttgatta tgtgaagcaa caagggatat ttatgatcgt tattactgta atcggtatta
    22201 ttttgaatat cttgttaggt tattttggtt cgaggattac gactaatatc gtagcagata
    22261 ttcgagatga tttgttcgaa aagatccaaa gttattcgca tcaggaatac gaaaatctag
    22321 gcgtatcttc attgatcact cgtacaacaa acgatgctta tcagatcatg ttgttcctgc
    22381 aaaatatttt acgtatcggt tttatggcac caatgatgtt cattgtcagt ttgtacatgg
    22441 tcatgcgtac aagtgtgaca ctcagtttgt atgtagtagg tgcattacct ttactattat
    22501 tggcagtcgt agcgattgct aaggtgtctg aaccgttatc taaaaagcaa caaaaaaact
    22561 tagataaaat caacagtatc ttgcgggaaa atctttcagg attgcgtgtg attcgtgcat
    22621 tcgtcaatga aaagtttgaa gaaaaacgtt ttgctgtagt caatgagaat tatgcttcta
    22681 gttcaaaaag cttattccgt ttgatggctg ttgcacaacc gggtttttat ttcttattta
    22741 atatcgtaat ggtattgatc atctggagcg gggcagtgca aatcgatgca ggcgagcttc
    22801 gtgtaggaga tttgattgcg tttatcgagt atatattcca tgccttgttc tcattcatgt
    22861 tatttgctag tgtgttcatg atgtatccaa gagcagctgt ttcagctgga cgtatccaag
    22921 aagccctaga tgccaaacct tcgattgtag aagatccaaa tggtatatct gatacaaaaa
    22981 ctaaagggta tatcgaattt caaaatgtaa catttgctta tccaggccat tcgcaagagc
    23041 cagtgatacg aaatgtcagt tttacagcct ctccaggaga gaccgttgct tttattggaa
    23101 gtaccggtag cggaaaatct acgttgatcc aattgatccc tcgcttttat gatgtgagtg
    23161 aaggaaagat cttgatcgat ggagtagacg tgagagatta tcggctaagc aagttgcgag
    23221 ataagattgg gtttattccg caaaaagcgc tgctatttac tggaacgatt gcagataatt
    23281 tgcgatacgg gaaagaagat gctacgcaag aagaaatgga acgtgcagct gagattgctc
    23341 aagcttcaga attcatttct cgaaaaccag atggatttaa tgaacattta tcagaaggtg
    23401 gttccaattt ttcaggtgga caaaaacaac gattggcaat agccagagcc atcatacgcc
    23461 gtccggaaat ctatattttt gatgacagct tttctgcatt agattatcaa acggatgcga
    23521 agttacgtgc tcgtctgaaa aaagaaacga cagaatctac tgtattgatc gtagcacaac
    23581 gtgtgggaac gatcatgcat gcagataaga tcatcgtatt aaatgaagga gaagtcgtcg
    23641 gtatggggac gcatcgggag ttgttggaaa aatgcccgat ttattatgat attgccgctt
    23701 ctcaattatc aaaggaggaa ttagcatgaa aaaaaccttt tcttccttaa aacgtttagc
    23761 tcgatatatc agaccgtatc gtgagacttt tcttttagtg atcctattta cagttctgac
    23821 agtggctttc aacgtggctt tgccatatgt tacaggactt cctactacag aaatcagccg
    23881 taatattgcc aatggcgaat caatcaattt ttcttatata ttcaaatgtc tcatttggat
    23941 cacagttgtt ggaatagggt attgtatttc gcaattactt tctggtgttt tgatgacaaa
    24001 tgtcgttcaa agtgccatgc atgatttgag aagagacata gaagaaaaaa tcaatcgtct
    24061 tccggtttca tatttcgata aaaaccagca aggaaatatt ttgtctcgtg tgacgaatga
    24121 tgtagatgca gtcagcggtg ctttacaaca agcttttatt ggtatcgtaa atgcgatatt
    24181 gggaattacg ttggctgcag caatgatgtt ttatatccag ccgatgatgg cgctgatctc
    24241 tatgatcatg attccaacat cgatctggat ctcaaaacga gtgatcaatg cttcgcagaa
    24301 atattttcaa agcatgcaga attctcttgg tgagctaaac ggctacgtcc aagaaaatat
    24361 gacaggtttt agtgtattga aagtatatgg acgcgaaaaa gaaacattgg aaggattcaa
    24421 aaaagttaat cacagcttga aatactttgg gtttcgtgca gcattcattt ctggcttgat
    24481 gatgcctcta gttcaattaa ctgcctatgc tacttatatt ggtatggctg ttctaggaag
    24541 cttttatgtt attactgggg cgatcgttgt cggtcaacta caagcattca tccaatatat
    24601 ttggcaaatc agtcagccaa tggggaacgt gacacaattg tcttcagttt tgcaaagtgc
    24661 atcagcggca acgaaacgtg tattcgagat tttagatgag ccagaagaaa aagaaaacaa
    24721 agtagatgct tcactgccag aaacgatttt aggagatgtg acattcgatc atgtcgattt
    24781 tgcatatgat ccgaagaaac cattaatcca agatttgaat tttgaagtga aagctggtca
    24841 aaccgtagct gtggtcggtc cgaccggtgc cggcaagacg acattgatca atctattgat
    24901 gcgtttttat gacgtagaca aaggagcaat aaggattgat ggaatcgaca ccaaatcaat
    24961 gagtcgcagt gatgtgcgtt cgctatttgg tatggtcttg caagatgcat ggctttatca
    25021 aggaacgatt gcagataata tccgcttcgg aaagcttgat gcgacagatt atgaagtagt
    25081 tgatgctgcg aaaacagcaa acgtagatca ttttatccga acgatgccag atggatatga
    25141 tatggccatc aattctgagg gagacaatgt gtctttagga caaaaacagt tgctgacgat
    25201 tgcccgagca gtggtctccg atcctaaaat cttgatttta gatgaagcaa caagttcagt
    25261 agacacccgt ttagaagcgt tgattcaaaa agcaatggat cgcgtgatgg agggacggac
    25321 aagtttcgtc atcgctcacc gtttatcgac aatccgtgat gcagatctga ttctagtgat
    25381 ggatcaaggt cagatcattg aaaaaggaac gcatgagagt ttattggcac aaggtggatt
    25441 ttatgaaaaa ctgtataaca gccaatttgc tgaggaagca gaataagtca tataagaata
    25501 aaacagttta aaacttattt tttgttctct attcttgata atggtaaagt aaagttatca
    25561 agaaaagtga ggacacggag atggaacagt ttttaaatga aaaaattact gaagccaaaa
    25621 gagaagttga tcgtttaacg cacgtctgtc agacgaagcc ttttgattca gctgattttt
    25681 ttgaaaatga gtttcaaatc gaacgcttgg cagcacaaat agaagtgtac gaattaattt
    25741 taaaaaaagt aatagaagaa aatatagatg tacaataaaa acaggtaaaa cgtcagttac
    25801 cagatactgt aaatagacat ctggcctgag caggttttac ctgttttttt tcttatgaaa
    25861 taaatatatg ctatgatacc tttattcaat aggattagtt tctacaaaaa atgaaaggag
    25921 aaaaataaat atggaacggt ttatagcatc aaatgccaca gtgattggtg atgtgacatt
    25981 gtcagaagat gtgacgatct ggtatcaagc tgttttacgc ggggacagta attggataaa
    26041 aataggacaa agaacgaata tccaagatgg tacaatcatc catgtcgatc atgatgcacc
    26101 tgtagatatt gcggagaatg tgacggtagg acatcagtgc atgctacatg gctgtacaat
    26161 cgaaaaaggg gcgctgatcg ggatggggac cacgatattg aatcatgcgg ttattggtga
    26221 gaatagtttg attggtgctg gatcattagt aacagaagga aaagttattc ctcctaatgt
    26281 cttggctttt ggtcgcccag ctaaagtgat ccgtcctttg acaaaagaag aaatacaaaa
    26341 aaacaaagaa aacatccagc attatgtcga gataggtcgg atgcatgcgc aaggaaaatt
    26401 caaggagcaa agataggcag tagcatccat aaaataaaaa aggatggtca gtttgagttg
    26461 accatccttt tttatttggt ttagataaga gagcttaaaa aagcttcctt tgaaacagat
    26521 taatttaatg aagaactgtt ttccatgact tcatcgatca atccatatgc ttttgcttgt
    26581 tcagctgtca tatagttgtc acgatctgta tctttttcaa tcacttcaag cggttgtcca
    26641 gtgcgttcag ctaaaatttt gtttaagcgt tcgcgtgttt gcaaaatatg gcgtgccgca
    26701 atttcgattt cagttgcttg cccttgagcg ccgccaagag gttgatggat catgatctca
    26761 gcatttggta aagcaaatcg tttacctttt gtaccagctg tcagcaagaa gcttcccatt
    26821 gaagcagcca tacccataac aattgtttgg acatctgctt tgacaaagtt cattgtgtcg
    26881 taaatagcca tacctgctgt aactgaacca ccaggagaat tgatataaag atagatatct
    26941 ttttcagaat cttgagcgtc taagaaaagc aattgggcga tgatcgaatt agcaagatca
    27001 tctgttactt ggccgcttaa catgatgata cggtctttca ataaacgtga gtagatatcg
    27061 taagctcttt cacctcgtga agattgttca atgactgtag gaattaaatt cataatagag
    27121 ttcctccttg atttcgttta gaaatagtgt accataattt agtttctaat acctaaaatg
    27181 tagtatacat ttttggtcaa aaaaggtcaa ataaaaaact taggcaaatt agtcgcataa
    27241 acccgcttta tggtatgata agcagagaaa caaaacaagg aggaacaaca gattgatagt
    27301 tggtattact ggccttatcg gagtgctgac aggtgctgta gttgtctata tcggcatgat
    27361 actgcaaatg cgtcacttgg aaaataaaga acagcgtaga cgtgaattgg aattgaaagt
    27421 caaagaaatc gagacgctga atcaattaaa taaaaagatc aacgagatct tgcaaaaacg
    27481 gacaacgctg atggaagaat atgtcagctt tgatgcattt gatgattgtt atatcacgat
    27541 tgacgatttt gcgtatctcc aatcattttc agcgcaaaat agtttttatc tgccaaatta
    27601 tattttagac gagctattta agaatatctc ccatcgaaaa gtgattttat ctccagatga
    27661 aacaatgaag attggtggat atgcttataa aggcggacgc gtgattttag agaattttac
    27721 ggatagtctg actgaaatga tcaatgaaaa aaaagctcag ttgaagacta tgacgcgcca
    27781 gccgattggt ttcttctcga aagatgatgt ttggagctga tattttgaag cattgccgtt
    27841 gcacatgaga gcgtggtaat gctttctttt tttcttaatg atacaataga actgaacgga
    27901 gtgaagaaag atgttacaaa tctatgccca tcgcggaagc agcggcaccc acccagaaaa
    27961 tacgcttcct gcatttgcag aagctgtacg agtaggtgca gatggtatcg aactagatgt
    28021 ccatctaagc aaagatggtt atttgatcgt tatgcacgac gaagaagtgg accggacgac
    28081 taacggtaaa gggctgattc gagaaaaaac attggaagaa ctcaaaaaat tgaatgcagg
    28141 gggcaaattc agcaaagaat ttccagcagc taaagttcct gaattgagag aagtgttagg
    28201 actgctcctc caaaaaaatt accgagggat gctaatgatt gaaattaaaa ctgatcagta
    28261 tgaatatccc ggtatcgaag aaaaattagc cgaaacatta aaaagtagag attggccttt
    28321 tcaacatgca tattgcagct ttcatttgga ttctttggaa aaaatcgcat tactggaacc
    28381 ggagatacag ctggacttat tgatgagtac ttctgataaa aagatccaac tagcagcaga
    28441 aaccccttat atagaaggag tacatccaaa aaatacttgg ttgttcgaac atccagtcca
    28501 ggcagctgat tttccaaaag caattcgtgc atggacgatt aacgatgaag aagaaatggc
    28561 caaatgtcaa gaactcaaaa tagatggaat aataactgat tttccggaga aagctcttcg
    28621 gtggaaacaa tcaacgaaaa gtaggtaaaa tatggaaaaa gtattagtga tcgtcggtcc
    28681 gacagcagtc ggtaaaacag cgctaagtgt ggaactcgca caactatttg gaggagagat
    28741 catcagtggt gattcattgc aaatctataa aaatctggat atcggtacag caaaaatcaa
    28801 agaagaagag atgaataatg ttccgcatca tttgattgat gtgatcgaac ctactcaacg
    28861 ttactcagcc gctgattttc agcattcagg tcgacaattg attaccgaga ttgcgaaccg
    28921 agggcaccta cctatcatag ctggtggtac gggtctctat atccaatccc ttctatatga
    28981 ttatcagtta ggagcgacag aagaagcgaa tcctgagatt agaaaaaagt atgaaacgtt
    29041 agctgaaaca attggttccc aagcattatg gaatcagttg aaagaaaaag acccggcagc
    29101 tgctgagaaa atccactgga acaatcaacg caaagtgata agagctctgg aagttttaga
    29161 gacgacagga catagtatca cggcacctaa agaagagcca aagcaattgt atgattacct
    29221 catgattgga ttagatacga agcgtagtat tctttatgaa cgaatcaatc aaagagtcga
    29281 tttgatgcta gcagaaggtt tagtagcaga agcgcgtaag gtgtatgagc ttggagaagt
    29341 ccaggccagt caaggtattg gatataaaga atttttccct tattttgaag ggaaagaaac
    29401 attggaagaa gcaacagaac aagtcaaact acattcgaga agatacgcga aacgtcagtt
    29461 gacgtggttt cgtaaccgtt tggatgcaca gtggtttgat cttataagtg agccaaatca
    29521 aaaaagtcag attgagcaaa caatcaaaaa atggctggag gcggaataaa atggaagaaa
    29581 aagtgatcat cgtaggggta gaaaccgaag caaaccagcg ttacttttca gagtcaatgg
    29641 aagagctagt tcagctgacg aataccgcat caggagaagt cgtattcaca attacacaaa
    29701 agagaccaca agtggatcgg caaacgatta tcggtaaagg gaaattacaa gaattagttc
    29761 aacaagcgga tgcgcatgaa gcggatttga tcatctttaa tcatgagcta actccgagac
    29821 agagccaact gattaccgat gctgttggtc ttccggtaat tgatcgcgtg cagcttatct
    29881 tagatatttt tgcgatgcgt gctcgttcga aagaagggaa gctgcaggtt gaacttgctc
    29941 agttagaata cctgctgcct cgtttagtag ggcaagggaa aacattatct cgtcttggcg
    30001 gtgggatcgg aacaagaggg ccaggtgaaa caaagttaga aacagaccgc cgacatatac
    30061 ggaataaaat ttttgctgtt aagaaagaat tgaaagaagt agaagctcat cgagagcgca
    30121 atcgccaaaa aagaaaaaat agcgagattt ttcagattgg acttattggc tacacgaatg
    30181 ccgggaagtc aactatcttg aatctattga cacaagcgga tacttattcg aaagatcaat
    30241 tgtttgcaac gttagatcca ttgactaaga gatggcgttt tgctgaagga tttgagatta
    30301 ctgtaacgga taccgtcggg ttcatccaag acctgcctac gcaactgatc gatgctttcc
    30361 attctacatt agaagaaagt caaaatatgg atcttttgct acatgtagtc gatgctagtt
    30421 ctccagatcg tattttacag gagcagactg tcttaaaatt gatggatgaa ctgaatatga
    30481 aagaaatgcc gatactgact gtttacaata aggcagacca gatcgaccca gcgatgttca
    30541 cgcctacgct attcccaaat gtattgattt ctactcagac gaaagaaggt aagatggcgt
    30601 tgatcgaagc aatcaaacgt cagctgatgg aactaatgac cccttataca ctttctgtgc
    30661 caagcaacga aggacagaaa ttaagcgagt tgagaagaca aacaatggtt ttggacgaag
    30721 cgtacatgga agaaacaaac gaatatgaag ttaaagggtt tgcaaaaacc aattcaaaat
    30781 ggttaagaaa aaaccaataa aattttattt ttttaa
//
LOCUS       DADVXU010000021        29935 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.023, whole genome shotgun sequence.
ACCESSION   DADVXU010000021 DADVXU010000000
VERSION     DADVXU010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 29935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.023"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            320..1189
                     /locus_tag="KB953_000940"
     CDS             320..1189
                     /locus_tag="KB953_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HBB6865935.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1398..2423
                     /locus_tag="KB953_000941"
     CDS             1398..2423
                     /locus_tag="KB953_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HBB6865936.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2462..3412
                     /gene="manA"
                     /locus_tag="KB953_000942"
     CDS             2462..3412
                     /gene="manA"
                     /locus_tag="KB953_000942"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HBB6865937.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3506..5209
                     /locus_tag="KB953_000943"
     CDS             3506..5209
                     /locus_tag="KB953_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBB6865938.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5211..7244
                     /locus_tag="KB953_000944"
     CDS             5211..7244
                     /locus_tag="KB953_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBB6865939.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7255..7695
                     /locus_tag="KB953_000945"
     CDS             7255..7695
                     /locus_tag="KB953_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBB6865940.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7895..9022)
                     /locus_tag="KB953_000946"
     CDS             complement(7895..9022)
                     /locus_tag="KB953_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6865941.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLLDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(9025..9702)
                     /locus_tag="KB953_000947"
     CDS             complement(9025..9702)
                     /locus_tag="KB953_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6865942.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9966..11732
                     /locus_tag="KB953_000948"
     CDS             9966..11732
                     /locus_tag="KB953_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HBB6865943.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11747..12184
                     /locus_tag="KB953_000949"
     CDS             11747..12184
                     /locus_tag="KB953_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBB6865944.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12197..13348
                     /locus_tag="KB953_000950"
     CDS             12197..13348
                     /locus_tag="KB953_000950"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBB6865945.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13410..14108)
                     /locus_tag="KB953_000951"
     CDS             complement(13410..14108)
                     /locus_tag="KB953_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HBB6865946.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(14101..15453)
                     /locus_tag="KB953_000952"
     CDS             complement(14101..15453)
                     /locus_tag="KB953_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HBB6865947.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(15676..17076)
                     /locus_tag="KB953_000953"
     CDS             complement(15676..17076)
                     /locus_tag="KB953_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HBB6865948.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17237..17974
                     /locus_tag="KB953_000954"
     CDS             17237..17974
                     /locus_tag="KB953_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HBB6865949.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            18099..18578
                     /locus_tag="KB953_000955"
     CDS             18099..18578
                     /locus_tag="KB953_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HBB6865950.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18575..19342
                     /locus_tag="KB953_000956"
     CDS             18575..19342
                     /locus_tag="KB953_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865951.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19572..20141
                     /gene="lepB"
                     /locus_tag="KB953_000957"
     CDS             19572..20141
                     /gene="lepB"
                     /locus_tag="KB953_000957"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBB6865952.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20245..20847)
                     /locus_tag="KB953_000958"
     CDS             complement(20245..20847)
                     /locus_tag="KB953_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBB6865953.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            20953..21422
                     /locus_tag="KB953_000959"
                     /pseudo
     CDS             20953..21422
                     /locus_tag="KB953_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            21528..22112
                     /locus_tag="KB953_000960"
     CDS             21528..22112
                     /locus_tag="KB953_000960"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HBB6865954.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            22115..23188
                     /gene="prfA"
                     /locus_tag="KB953_000961"
     CDS             22115..23188
                     /gene="prfA"
                     /locus_tag="KB953_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBB6865955.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            23181..24020
                     /gene="prmC"
                     /locus_tag="KB953_000962"
     CDS             23181..24020
                     /gene="prmC"
                     /locus_tag="KB953_000962"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBB6865956.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            24013..24192
                     /locus_tag="KB953_000963"
     CDS             24013..24192
                     /locus_tag="KB953_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865957.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            24214..25239
                     /locus_tag="KB953_000964"
     CDS             24214..25239
                     /locus_tag="KB953_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBB6865958.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            25430..26674
                     /locus_tag="KB953_000965"
     CDS             25430..26674
                     /locus_tag="KB953_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBB6865959.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            26827..27774
                     /locus_tag="KB953_000966"
     CDS             26827..27774
                     /locus_tag="KB953_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBB6865960.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            27908..28537
                     /gene="upp"
                     /locus_tag="KB953_000967"
     CDS             27908..28537
                     /gene="upp"
                     /locus_tag="KB953_000967"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBB6865961.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(28676..28918)
                     /locus_tag="KB953_000968"
     CDS             complement(28676..28918)
                     /locus_tag="KB953_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865962.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            29039..29111
                     /locus_tag="KB953_000969"
     tRNA            29039..29111
                     /locus_tag="KB953_000969"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29072..29074,aa:Thr,seq:cgt)
     gene            complement(29230..29868)
                     /locus_tag="KB953_000970"
     CDS             complement(29230..29868)
                     /locus_tag="KB953_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBB6865963.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT        10186 a         5285 c         6085 g         8379 t
ORIGIN      
        1 caaaattttt cttactactc gaaactaagc gactttttca caacctcttc cttaaacggt
       61 gttcaaaagc ttgcatctgc ggtaataagc ccaaccaaac gaaaaatagg aggagctatt
      121 tttgtttggc tgttcttatt acttgatgca gaacagcttt ttcacaacct ctttttataa
      181 gagtttttac tcattaattt acaaaaaaca agcaaaatgc gaattttctc acattttttt
      241 taacaaaatc aaagttattg cttgaaagaa gaagaatcca tcgttaaaat aaagcttgac
      301 gtagagggga gcaagaaata tgaaagattt tcaattgggg gctgccacct atttaaaaag
      361 tgtagccatt cgggtgaaag accgagataa aatgattgat ttttacaaga acgttgttgg
      421 ctttgatttg aaaagagaag aaaacgaatt agccatttta ggaacagaag aagctggaag
      481 tgaaatgttg ctgctggaag aaagtcctag agcgaatgat cacatggggg tgatcaaaaa
      541 gctgaaccgt ttttcattga ttatccctag cacagaagaa atggcagata tcctttgtcg
      601 tatccgtaaa atgaattatc caatcaaaga agctttagaa gatcatggac gtatggggat
      661 tttgctcgta gacccagaag agaatgaatt ggaaatttac catgaagaga ataagaacga
      721 ccaaagagaa attccagaaa caatggatca agaagcatta ttaagaaaat ctgaagaaaa
      781 attcagtaaa ctatcaacag gttcttattt tgaaaaaatc catttaaatg ttactaactt
      841 aacaaaagaa cgccaatttc ttaatcatgt acttggtttg aaggtacata atgaagaaga
      901 gggcatccat gtattgaatg aaggagattt ccatgttggt ttgacagaag ctagcggagg
      961 cgcaattgat ttgcctacag atgaagtgat tggacttgat tttctgaaat tcatgatcga
     1021 tactgaaagc atggaaggac tgattgctca cttgcagcaa ctatcagttg atttcttctt
     1081 agacaaaaag cacacagtac tcacaatcta tgatgctata ggaatcgaat ggtggttcgt
     1141 cagtgagcca aaagaaaaga ataaaactga cattcgatgc gacgcttaac tttttctttc
     1201 ttaacgattg tcgcatgtct cgtcatttta tgaacggtca tactatcgct tattctagag
     1261 atagaaagag aaaatagtat accgggggaa actgcgcggt ttttcgcgca gtttttttgt
     1321 gtaaaaaagg ttgattcctc atagattaag ctttaagctt ggactgtaag aatctgatag
     1381 aaaagcagga gaaaaagatg tatccacaca cgaaacagct tattttgaaa aagatggaag
     1441 aaggatttta tccaggtgta gcagtcgcct tcataaaaga aaatcaagta gaaacattta
     1501 cagccggctt tgcccaagtc gttcctgaaa agcagctgat gacagaagat ctgttgtttg
     1561 atgcagcctc actgacgaaa gtcgttgcaa ccacttcatt agttttacgt ctattagaag
     1621 atggaaagat agaactcgat caaccgcttc ataaatatct tcctgaattt gaaaatcaga
     1681 caatcacttt gcgtcattta ctgacacata cttctgatat caaaacatgg ataccaaaca
     1741 gagataagct atctcaggaa gaattgatcg ccgcttattt gaagctagaa ccaggtgagc
     1801 aactaggaaa agaagtaaaa tatacagatg ccggtacgat tcttcttggg ttgatgctag
     1861 aggaaattta ccaagaaact gcagtggaaa tctttcaaga acaagtattg gaaccattag
     1921 gaatgatgaa cagcttattt cttccttttc cttccaaaaa aatcgttccc acagaacaac
     1981 tgccttcggg tgaggttttg cgtggacaaa ctcatgatcc gaaagcaaga gttttggctg
     2041 aacatgcagg taacgcgggt ctctttattg atttgaaaga cagcattact tttgtccaaa
     2101 tgtatttgaa caagggaaaa atccgtaatg gtcgtttttt agaagaagag acgattgatt
     2161 ctttactgca agatcaaaca ccgaaaaaaa atggctatcg ttctttagga tgggacttaa
     2221 aaaaggatgt agaagacggt catgcgattc tttttcatac tggttataca ggtacgtttt
     2281 tcttgattga tataaaaaca caagaagcgt tgatattttt gtcaaatcgc gtacatcctg
     2341 tcgatcatcg tgcggagtat ctcatatgtc gagatgaaat tgttgaaaac tttttgaaag
     2401 agaaagcggt attaccgaaa taatgctata atagctatga aaaatgagag gagtttgaat
     2461 tatgcaggaa cctatgtttt taaaaccagt ttttcaagaa aaaatttggg gaggaagtcg
     2521 tttacgttca gtctttggtt ttgatatccc taacgataaa atcggagaag attgggcgat
     2581 cagtgcgcat cctcatggtg tcagtgtagt agaaaatggc gaattcaaag ggaaaagatt
     2641 agatgaacta tggtcagaac acaaggaatt gtttggccat ccttcagaac cagtgtttcc
     2701 attattgatc aagatcttag acgcagaaga tgatctatct gtacaagtac atccagatga
     2761 tgcctatggg atgaaacatg aaggtgaatt aggaaaaact gaatgctggt atatcatcga
     2821 tgctgaacct ggtgcagaaa tcatctatgg tcatcatgca aagacaagag aagaactagc
     2881 tgagatgatc aaagatgggc gctgggatga ccttttgaag aaagtaccag ttaaaaaagg
     2941 tgatttcttt tatgtaccaa gcggaacgat ccatgctatt ggtaaaggaa tcatgatttt
     3001 agaaacacag caaagttctg acacgactta ccgcgtgtat gactacgaca gaaaagatgc
     3061 gcaaggacaa acaagagagc tgcatatcca acaatcgatc gatgtaacaa ctgtccctgc
     3121 gaaaacacca gaattacaaa tcaaagaagt ccgaaaaggg aattcatcga tcgtcactta
     3181 tttagaaact gagtttttca atgtatatga gtgggatatt aaaggaatca catctttcaa
     3241 aaaacaagcc ccttatacat tgatgacggt aatcgaagga gcaggagacc ttgtcgttga
     3301 cggaaaaaca tatccattag aaaaaggaac aagctgtatt attccaaatg gcgtttctga
     3361 gtgggcggtc caaggtgaac tagcaatcat tgcgtctgaa ccaggcaaat agttcccaga
     3421 tagctgaaag cagcagacgg tttttcgttt gctgcttttt ctacataaaa tccgtccatc
     3481 aataaataga agtctggtga aaaagatgga atacaaatgg aaagcaaaca acagcagtcg
     3541 cgtgtctatg aaacaactga gaaaagctgg acatcactta aatgaaatga tcatgccaaa
     3601 tctaagtctg tttatcgctt ggggaatctt gtcattagca acaaaaggtg tgtctggcga
     3661 tttgcacacc acattagaag aggtatgcca ttggatgatc cagctgcttt tacccatctt
     3721 gatcagttat tcaggcggga aatcggtagg gggacaaaaa ggggcagtcg ccggtgcaat
     3781 tgcttcttta gggttgatcg ctcgttccaa tgctccacaa atcgtaggag cgatgattat
     3841 cgggccgctt gccggcatcg tatatgaaca atttgtttct agattccatg tgaaattcaa
     3901 ggctggatat gaaatgcttg tcagcaacct actagtagga ctgactggta gcgttatttg
     3961 tataattgga atcgttttta ttgaaccagt tttaaatagt gttcacttgt tgctcgcctc
     4021 cgttgtgtct tggctagtca caatgaatct gttaccactt gtcagtctga tcttagaacc
     4081 tctaaaagta ctatttttca ataatgcaat caatcatggt gtattgacac ctttaggaat
     4141 cgaattctca caaacagtcg ggtactcttc cttatttttg atggaggcaa atcctggacc
     4201 tggtttaggg atcttacttt ctatcctttg ttttgcagaa aaacaggaaa aagtaaatgc
     4261 gagtggagca ttgatgatcc atgcgattgg cgggattcac gaaatctatt ttccatttgt
     4321 cttgctgcga ccgtatctat ttttagctgt aatggctggt ggggcaagcg gcacgttgat
     4381 atttcaatgg ttagatacgg gattagctgc acctgtatcg cctgggtcac tactcgtgat
     4441 ttttgccaat gcgccttatc cacaactagc tggggtaagt atagggatag caatcagtac
     4501 atttgtgacc ttcatttgtg cagcgatact attgaaatta ccaaataaag aaaaagagaa
     4561 caaaacggtc aaagaaaggg gagaaatctt ggaaccatct atcattcatt ccgtcatttt
     4621 tgcttgcgac tcaggtatgg gatcaagtgc tatgggagcc gccctgctga aaaagaaatt
     4681 agcagcagaa aaaatcgatc tttctgtcgg atacagctct atctatgaat tgaataacca
     4741 agtaaatcag ctggttatcg tgcaacatga actagcgggt cttgcaagag aaaaagtacc
     4801 tcaaagtcaa atctatgtac tggatcactt tttggaaaca gatatcttgc tgtcacgaat
     4861 aaaagaagcg atccaatttc caaagcggga agaaaaaaaa gagatagcag atatcacaga
     4921 acaaaaaacg tcgcctgcta atattgtatt tttgtataaa aacaacagac gcggctcaca
     4981 aactatgggc atgacgattt taagtcaatt agcaaaaaaa gcgaaaaaac agtttgttat
     5041 ctcaaaagaa tcaatagaaa aaatcaaaat ccaagaagaa acgatttata tcgcacaaag
     5101 agaactagct gtaactgaac agctagaaga aaagatccct cggctagttc tagttgaaca
     5161 ttgggtagct acgaatgaat acgaaaaact cgtgaaggga gaaatataag atgtatttat
     5221 cgcccagaga aaaacaatta ttagcagaat ttctcagtag cccgtcacct gtatctatcc
     5281 aaaaaatgat gaatttattg aaggtcagta aacggactat ttaccgcgaa ttagataatc
     5341 ttgaagtctc attgaaatcg gtaaatgctt ctttgaaaaa agcagctaga ggctcatttg
     5401 tcattgaggc atcagaagaa gtattagagc aattaaaaga aaatacagat gggaaaagta
     5461 atgacttatc cactgctaaa cgacaacacg cgattttagc cgagttgtta ctgacgacag
     5521 agccgttgag tcttgctcat ttcatggaat catatcgcat cagtaatacc actttttatg
     5581 cagatatcaa acagttggaa gaaagtattc gccaactgcc tttggaaatc attcggaacc
     5641 agggatacga gatcgaagga ccggaaaaat accgccggct tctgacagcg aatgtattag
     5701 aattagaaat caatgaatat gaactttttc atagtatttc tttcgatgca tcactgaatt
     5761 atttttttcg attcgttgat ccgcaacatc tatctcttgc acgcaaagta atcggggaag
     5821 aattgatcca aaaaaagacc aatctaagtg atagaaagct tgaacatttg gttttgatgc
     5881 tgacattgac gatggagcgt gtgacgaaaa atcatttact gacaaatgag acatataata
     5941 gtctggcaaa caaagaatta ctacagacag ctaaacgatt gttttcacgg atcgcttctg
     6001 agacaaaaca actttatcca gtaaatgaga tcgtcttttt tgcaggatta ttaaatgact
     6061 tcacgaactc atttgatcag aatttctttg aagagacgtt cgatacacag cttgcttatt
     6121 tagtaaaaca actgattcaa gaagtaagtg aagagacgaa tgtccatttt tatgaagatg
     6181 aaactttgta caaaatgctg ttgactcatt tatctggtgt gttttcaaga gccgttttac
     6241 aagaagagac actgaccaat ccgattttag aaaaaatcat ggttcaatac gaggaagttg
     6301 cgaatgcaat ccgtaaagca gtagagcgga tttttcaaga aaaaaaatta tccgaagaag
     6361 agatcgctta tatggtcctt cattttgcga attcattgga acgaagtccg aaagaaagca
     6421 atatcagtat tgcaggattt tcgccaagtg gattagcttc tactagcatg ttggaactac
     6481 gattgaaaag gtattttccc ttcatccaac aaattcattt ctatcggatt gctgatttaa
     6541 aaaaaatcga tttacaagaa gaatatgatg ttgttatttc tacatctatt ttaccaggat
     6601 acggtggaaa atatatttta gtctccccgc tactgctaga agatgaagtc aagcagctaa
     6661 aagaagcttt ttcgcatatt gagaagaaaa aaaggcaaaa aattacgtat aacagcaata
     6721 acgagtggat gatagaagat acttatgagg aaacaatgca tttcatggat caaatcaatc
     6781 aattactcgg tcgttttttt atccaagaac tggataatcc tgagacgatt tccgaaacga
     6841 ttcgcagctt atttccttat tttcctgaag gactagtaac ggatgaagag gtggttcatc
     6901 gccggctgct taagcgatat gaacaagcac ctatcggtat tcctcataca aatatggggt
     6961 tattccatac atccagctca ttgatttcta aaccgatctt ttgtattttc aatttgaaac
     7021 atccgttgac cattgaaggg atggatagac agccagtcga tttgtcccgt atgttagtta
     7081 tgctggcgcc ttcgccaatc caagaaaaag acgggattct tttaggaaag atcagtggag
     7141 caatcatcat gaatgacttg aacaccgaga tcttccgctc aggaaataaa gaaatcgttt
     7201 atcagttgtt agcgaaaatc ctgattgaag aaataaagaa atgaaggtga aaagatgata
     7261 cagctaccag cagaaaaaat attgctcgat caatcctatc aaaattggaa agaagcgttg
     7321 accgagatcg gccagtggat gacccaacaa gggggaatca aaccggcata tatccaatca
     7381 atgatcgaaa gacaagaaaa agcgagtgtc tatatcggga atttcgtagc cttgccacat
     7441 gggactgaca aagatgaaga agtagtagaa gaaggaatct atttagtcca agtgcctgat
     7501 ggtgtggact ttggaacaaa agaggaacca aaaatcgcga cactcttatt tgtagtggct
     7561 ttaaaaaaag aacatcagtt agcggtgctc caagatttag cctttttctg ttcagacatc
     7621 gaccaagtaa tggcgctcag tgatgcggaa accattgaag aagtccaaat gatttttgaa
     7681 catggacata tataaccaat gttcacagta aaaaaagctg ccttgcatca agtaataact
     7741 tcaaccaaga taaaaataag aggagctatt tttgttggga ggttcttatt actgcggatg
     7801 caagcttttg aaaactattt attctagaaa accgtttatt tgaaataggg gacggtgaca
     7861 aggtttttgt caccgcccct attatttttg tgttttaata gctctttttt atatagatag
     7921 tgcccatacc tatgaataat aaaacgatac tccatatacc aagtgtcaag ataccattca
     7981 tgaaagtaac ctgttctgaa gctagtggcg agaaaatatc tcctcctatg atcacatttt
     8041 gaaaatcaac ataagaagtt ggcaagtaat gactgattga ttctatccat ccaatcgaag
     8101 aaaacaagcg atactctgct aagaaaagac aaagaagtgt acctaaaaga ttgactagag
     8161 aatgttcaaa taaagcagat agaagaaaac tgattgtact taaaaagagc acccacaaca
     8221 acaggaacag aaagctctgg aaaagatact catacatcgg caggattcgt atctcctgac
     8281 caatgatcgt tcctacagga taacggaagt ctcccaaacc atttttgatt attactagta
     8341 tagagacgat caaaaatggc atcacgagac ttagcatagc tgcaccataa taaatggtaa
     8401 aacggatgct atgtttcttt accatactat ccagtaatag attgactaga gagattacct
     8461 gctttctttt ttcataagag atgatggaag aaagaaacaa ggcggttata cataaaatca
     8521 aaaaactaga aatcattcct gaaaaaatat tttcgtagta atttgctaaa gagagatgcg
     8581 ctggcaagtc aaaaatagaa tatctttgga tctgatgttc tttcatataa cggagcaact
     8641 cgacacgttt ttgttgttca atgattggaa ttccttgtct ttgaccagaa atcagtcgat
     8701 ccagtatatc tttttcataa tgataagttg cttcgaccat ttgctggaca ttccctgtgt
     8761 tttttgcatg taagattgct tcgatcagtt tgttgctttt ctctaaactt tccacaaatt
     8821 ctttctcatt ctcatcttct ttcaacgttt caatcaaacg ttgcatctct tcatagttat
     8881 gattcagttc atcgaattct gtataagcaa gtttatgatc aaaaaaaagt gggaatgcaa
     8941 agagcacgac caacaataga ggaaataaaa agtttacttt gttttttagt aagagattgc
     9001 gttcaaatgt aagtaaagac atagttacac ctcatataat ttacgataaa tagctaaaat
     9061 ctcttccata tttgaggatt ctgtgacgat ttctccattt tttagaaaac atgctttaga
     9121 acaaatcgat tggatattcg cgatagcgtg ggaagaaaga agaatcgtct tcccctcttt
     9181 ctgccattgt ctgatgaggc tattcaagat atcaatactt cctgggtcta atccgttcaa
     9241 cggttcatcg aataacatga cagatgaatc actgatcaag taagcagcaa gcaagacatg
     9301 ttgtttcatt cctagagaca tttttccaat cggcaaagta aggtactcag tcatcttcaa
     9361 tcgttggaaa acagtttggc attctacttt gctctgccaa tagtatttga tcaaagtcaa
     9421 ataatcaaca gccgatagat agtcggataa ctgattcgtt gattctagaa aaaagaaaga
     9481 ccggcgataa tctgtataag cagtaggaga gatgttgtga agggacaatt gatcataagt
     9541 tgttggcagc agttgggcaa tgccattcag caaagttgtt ttgccaaatc cgttcggggc
     9601 aatcaaagca gctacttctc ctgattctac taaaagattc aattgtcgaa tcacacgttt
     9661 tttaccacgt ttcatgttca tgtttttgat cagtaatgtc atagataggc ctccaatata
     9721 aaatttaata acataataga atggaaaaca tctaaattaa atgctaacaa aagttattgt
     9781 ttgcatatta taaaaaaatt aaaataacag ttttatttaa aattgaagtt ttctgatacc
     9841 gaaatctttt tattttgctg acatactcaa acctttggca caaagtttcg tgccaaagta
     9901 cgactatttc tctttttagt aagcggatac aattagagta tcaaaaggag agagggagag
     9961 acaaaatgga acggacgatt cccaaaaaca acatttcgct aaaagcgagg gtacaaaaat
    10021 taggcagtac tttatcaagc atggtcatgc caaatattgg agcactgatt gcctggggtg
    10081 tattaacggc tctatttatc ccagatggtt atttaccaaa tgaggctttc gctaccatgg
    10141 taagcccaat gctaacttat ctgattcctt tattgatcgg ttatactggc ggaaaagtaa
    10201 ttgctggtga ccgcggatca gttgttgggg caattgcaac gatgggtgtg attgtcggaa
    10261 cagatattcc aatgatgcta ggcgcgatga tcatgggacc tcttggtgga tatgccatca
    10321 agaaatttga tcagctattc caaaaacgga tcaaatccgg ttttgaaatg ctagtcaata
    10381 atttttcagc aggtcttatc ggttttggat tagcgttatt aggtttttct gcgattggtc
    10441 cagttgtcga tgcattgact caagcaatgg caaaaggtgt agagatcatt ttatctgccc
    10501 atttgattcc attgacaagt atttttattg aaccagcaaa aatcttattt ttgaataatg
    10561 caatcaatca tgggattttg acaccattgg gaactgaaca agtagcagag gctggaaaat
    10621 ctattctatt cttgcttgaa gcaaatcctg gtccaggact tggcgtatta cttgctttta
    10681 ctttgtttgg caaaggagca gcaaaatctt cagcaccagg agccatgatc attcactttt
    10741 taggcgggat ccatgaaatc tattttcctt acgttatgat gaaaccgtta ttattcttat
    10801 cagttatagg cggaggaatg tcaggaagtt tcattttcca attgatggat gctggactaa
    10861 gagcgccagc ttctccaggg tcgatcattg cgattctagc gatgacaccg ttcaattctt
    10921 accttcctgt tattttgggc gtgattgttg ccgcagctgt ttcgtttggt atttctgcag
    10981 taattttgaa agcggatgcg aaagatacaa atgaagactt cgaaaaatct gttcaagaga
    11041 caaaggcagc taagcaagct tccaaaggtg cccaaggtat tcctcaagga gctacattct
    11101 ctggcgtaca aaaaatcatt tttgcttgtg atgcaggaat gggatcaagt gcgatgggcg
    11161 cttcgatctt gagaaaaaaa gtacaggaag cagacttgcc tcaaacggtg accaatcaag
    11221 caatcagcca actgacagat gactcgaata cattgatcgt tactcaggtc gaactacaag
    11281 aaagagcaaa acaaaaagca ccgagcgcgc gttttgtctc agtagaaaac tttttgaatt
    11341 cacctcgata tgatgaaatc attcaagaat taagcagcaa gcagaaaaca tcagtttctg
    11401 aaattgagaa tgagccagca gaagagtttc tttcagcaaa catcaaacaa atcaaagaga
    11461 ttctacttgt ccacgatgat cgttccggat cagcaacaat ggggatgacc gtgctcaaag
    11521 atatattaga caagaaccat ttaactatcc cgttaagaaa agtcaatatc caagatttaa
    11581 aagatgaacc tgattcactg atcgtagcca aagaaagttt agcggaaaaa gtacagatgg
    11641 ctgcacctca atccattcat ttatcagtca acagcatggt caatccgcaa aaatacgaat
    11701 cgattgcaca atatttgaaa cagacagctt aagggaggaa aacagcatgc aaagtttgcc
    11761 attcgacatg atcgagctca atcgatcttt taaaaccaaa gaagaagcga tccgctattg
    11821 cggtcagatg cttgtagaag cagggtgcgt tgatgaggaa tatatcgatg ccatggtaga
    11881 tagagacaaa atgttatctg tttatatggg gaattttatc gcgattcctc atggaacaga
    11941 tgaagcaaaa aaatatgtga aaaaatccgg catctgtgtc gttcaagtac cagatggtgt
    12001 agattttggt accgaagaag aagaaaagat tgcgactgtt ttgttcggta ttgcaggagt
    12061 cggagatgag catttgcaac ttgtacagca aatcgcactt tattgcagtg atatggacaa
    12121 tgttgtccag ctagcagacg cgctcagcaa agaagaagta acggaaaatc tagcaattgc
    12181 ttaaggagag agaaaaatga gagcagtaca ttttggtgca ggaaatatcg gtcgaggatt
    12241 tattggagag gttttggcag ctaatggatt tgagattact ttcgtagatg tgaatcacac
    12301 gatcattgat gctttaaatg aaagaaaaga atacgaaatc gaattagcag atgaatcaaa
    12361 agaaaaaatc actgtaaaaa atgtcaaagg aatcaacaat caaacaaatc cagaaaaagt
    12421 aatccaagaa atcgctgaag cagatttagt tactaccgcc attggcccga atatcttacc
    12481 ttttattgct gagttgattg caaaaggtat ccaagaaaga gaaaaggaag gaagcacaac
    12541 atctttagat atcattgcct gtgaaaatat gattggcggt tcagcatttc tagaaaaaga
    12601 agtgtataaa tatcttccgg aaacatcatt tactgacaaa tatatcggct ttccaaatgc
    12661 agcagtcgat cgaatcgtcc cattacaaca ccatgaagat cctttattcg tccaagtgga
    12721 acctttcaaa gaatgggtca ttgacgatag ccaaagaaaa aataaaaaaa tccaattaaa
    12781 aggggtttta tatgtagatg atctagaacc atatatcgaa cgcaaactat ttagtgtcaa
    12841 tactggtcat gcgactgtcg cttatacagg tgccttgtta ggttaccaga ccattgatga
    12901 agcaatgcaa gatgcgttag ttgtggcaca attaaaatca gttttgcagg aaacaggaag
    12961 tttgctgatt gccaaatggg ggtttgatgc agagcagcat catgcgtata ttgaaaaaat
    13021 cattcatcgc ttccaaaaca aaaatatctc tgatgcgatc acacgtgtcg ctcggacacc
    13081 attacgtaag ttaggttatc aagaaagatt tacacgtcca gtgagagaac tacaagaaca
    13141 taacttgact tgcccacatt tgacggcaac aatggggatt atttttaatt actatgatcc
    13201 tgaagatgaa caaagccgtc aattgcatga aatgaagatc catgaaaact tggaacaatt
    13261 gattcaagaa gtcacaggaa tcaatgatcc gaaaacaatc gggaatatca agcaaaacgt
    13321 gaaccgttat gccaagcaag tcgcttaaaa taggatacga aaaaggggaa tgtgacgaaa
    13381 tttttgtcac attccccttt ttcgaataac taggctgttt gtccttcaag atagggcaga
    13441 tccaattctt taccgtaacg ttgttctaaa gctaggcgga tcaaacgagc tgccaggttc
    13501 tcatttcttg caaggatagg tccatggaaa taagaaccga agacatttcg gtaaatgaca
    13561 ccttcagatt ggtcttcacc attatttcct tgaccttgta ccactttccc caaaggtcgt
    13621 tcaccttcac ctaagaaagt ccgaccattg tggttttcaa atccataata agtttcattg
    13681 aattcttcgt tatggataac gatatcaccg ataaaccggt tattttcctg acttaacgtg
    13741 tagtgatcca gtgcactgat tccttggatc ttttctccat tagctcccat gtagtagtga
    13801 cctagcaatt ggtagccacc acaaatggct aacatgacgc catcattttc gatatagttg
    13861 atcaaagaat ctttcttttt ctggatgtct tttgaaatga ttaattgctc aaagtcttga
    13921 ccaccgccaa agaaaaccaa gtcatattta tcagcatcaa aaggttcatg tatactaacg
    13981 atctccgtat ggcaagaaac ccccattttt tctgctatat atttcaacat caaaaggttt
    14041 ccgttatcac catatgtgtt catcaaattt ccataaaggt gggcgatatt gagttcataa
    14101 ttagccacgg ttcattcctc ctttgataaa tccttgagca gctagttctt tacgaagctg
    14161 caatacagca gtgtaagtag ctaaaatata gacatgctca gttggcaatt ctttgatttt
    14221 ttcgattact tctggtaatt cttttgtttc tgtcaattta ttttctggaa tcccggcaac
    14281 ttttagacgt aaagtcatat ctgtatgacg gtctcctcca gaaataacag ctgggatatc
    14341 catatcggca aagttttcaa aattaccatc ccaaatccag ctgacatcga ttccgtctgc
    14401 gtaattggca tttaataaag ccactaaaga aaatggataa ggagctagac caatcatatc
    14461 gattacttga ttgataccta caggattttt gacaagtact aatgtacatt ttttcccatt
    14521 gatattgatg acttcttgtc ggccgaatac tttctcgtca tatcctaaac cttgtttgat
    14581 tttatcagga tcgacattga agtactctgc cactgcagta gctgctaaag cattgtagac
    14641 attatacata ccgccaactt cgattccgta ttcatgacca tcgatcacga aatctgctga
    14701 cacattgtcc atccgtacca tatcagttaa tcgataatcc aattcaggac gtttgaagtc
    14761 acaatttgga cagtaatatt tccctaagtt tgcataagtg atcattttat aatgcaagat
    14821 gtgatgacaa tttggacata agacaccgtc tgtattgtaa tgagccattt gttcacgatc
    14881 ttcttcatgg tcaaagccat aatattttct aggattgact gtttcgatag aattgaagat
    14941 tggtgaatca ccgttactga taatcgttgc atttggtgac tttgcggcac cgtctacaat
    15001 cattttatag gtcgtataga tttctccata ccgatccatt tgatcacgga aaatatttgt
    15061 aaataagaac agttctggtt tcatatattc tgtgactttg ctcaaacttg cttcatctat
    15121 ttctaggaca gcaaactttt tctgtccttt ttttggtttt gcctgcaaga aagtcgatac
    15181 aattccctgt accatattcg caccagtagg gttggttaga acttcatcga attgctggcg
    15241 caaaatattg actgttaaag cggtagtcaa tgttttaccg tttgttccgg tgacaacgac
    15301 gacttggtag tcttttgcca aactatctag tatatgtgga tcgactttca gcgctagttt
    15361 acctggtaaa cttgatccgc ctttaaaaaa agtttttaat gcccattgcg acgtttttcc
    15421 aactgcaatc gcaaaatgac tgcgtattcc cattatgtaa cctccaaaaa cgatttgctg
    15481 acgaataaat tcaactaatc ataccatatg ttgaaaaaaa ggttaaattg cttttcttat
    15541 tcttaagaaa aggttacggc tgaaagtgca tgaaatcgga caaaatcgca tgatttaaca
    15601 aaaaaacagc ttgaaacaaa atgattgttt caagctgctg tgattttatt attccctctt
    15661 acctatattc ccattttaac cgataataat tttctcgctt ggatattcat agcctagatc
    15721 tcgtgtttct tttggcacga ataacatcaa tgtatatagc attccgatcc gcccaataaa
    15781 catcagtgta gcaatcgtga ttttgcccac aacagacaag tctccggtaa tccctaaaga
    15841 taaaccagtt gttccaaacg cagaagttac ttcgatgatg atcgagatca gcgtttgttc
    15901 ttctgtagca gataaaaaaa cgatacaaaa gaaacacata ccaagagaaa gcataaagac
    15961 aacgactgat tttcgcacat catcttgatc gattctccgt ccaaaaatat tgatgttatc
    16021 ttcacttttg aggaaggaat atagatacaa accgatgatt gctacagtag ttgttcgaat
    16081 cccgccgcct acagaactag gactgcatcc gataaacatc agtaaagaaa aaatgatcag
    16141 tgttgtgatt tgaaaatcac ccaaatcgtg aatctgcaga ccagcattac gagtcgtaat
    16201 cgaataaaac atcgaattga tccatttgac actcatattg gaatcttgga ataaatggtc
    16261 cttttccaat agataaatca agacagttcc cgaaacaaac aagataacaa aagctaacac
    16321 agctaatttg gtaaacaagg aaaaacgaaa cggaagcttc gcattggaac gtttgtataa
    16381 tagccattcc cggcactcca tcaatacagg aaaaccaatt cctccgacaa aaatcaaaaa
    16441 catgatcagg atcaagaaaa aatagtcatg tgcaaagggc ttgatggaat ccccagtgac
    16501 gtcaaaccca gagttcgtga cagccgatat cgcctgataa aaaccgtaaa aaacggcatc
    16561 acgccaacga tcaaaatagc ctctgtaata aaaataaata gaaaagaatg tcccaaaaag
    16621 taattgaaac cagattaaaa tagcaaaagt gatccgaatc agtcggacga taccagacaa
    16681 acgcggttga ttcatatccg tcatgatcag ctgtcgctgt ttcagtgtga tccgtcgttt
    16741 agaaaaaatg acaaaagcag tagaaatcat catgatcccc aaaccaccaa cttgaaataa
    16801 tatttccagc agtataatac cgttatcatt gaacacagaa ttgatatcaa aagtcgatag
    16861 tccagtcaca ctcactgtac tgattgccat gaacagcatg tccacaaaag aaacatggct
    16921 atctggttcg cgaaaaatcg gcatataaaa tagaacgagt gataagaccg tcatcaatat
    16981 ataataaaaa acgatgattt ggatcgatga gaaatgattg gcagcaaact tctgtcctcg
    17041 ccgctgcagc cttcgaaaga ataaatcaac atgcacagta ctatcctcct gacaatagtc
    17101 gtttctacca gtatacaagg ggaattagga atactcaaag aaattatcaa acagataaca
    17161 atacagcttt cgattaaaat agaaatggct tataataagg aaaatcctag aaaaaagaag
    17221 gaggaggaat aacaaattga ttaatgctca acaaatcgta gaaaaaatga aaaatcaaaa
    17281 aataaattat gatacagttc tgaaaaaaat gatcatgcaa tgggagaaag aagaagtaaa
    17341 gcctaagata ttaatacata catgctgtgc tccttgcagt acgcacacat tggaattcat
    17401 gtctcaatat gcagagatca cgttattttt ttcaaattcg aatattcatc ctaaaagtga
    17461 gtatttgcga agattacatg aacaaaaacg atttgtagac caatttaatg agaaaactga
    17521 ccatcatgtt gcgctaatcg ttgatgaata ccgtccaaat gaattcacca aaatggtttt
    17581 aacaaaccat cttgaaaacg aaaaagaagg tggtgctcgc tgcagtgcat gcttcaatat
    17641 gcgattagat cttgttgcgc aaaaagcgca agaactagga tacgattact ttggaagtgc
    17701 cctgaccatc tcgccaaaga aaaatgcagc tttgatcaat cagatcggaa tggatatcca
    17761 aaagatttac gggacacaat acttgccaag tgatttcaag aaaaacaaag gctatgaacg
    17821 ctctttgcaa atgtgcaaag agtatgatat ttatcgccaa tgttattgtg gttgcgtgtt
    17881 cgctgccaag cagcaaggat gcgatctgaa agaaatcaat cgggaagcaa aagcttactt
    17941 aaaaacatca accgtagttt ttgaggaaaa ttaaagaaat ctttcggaaa aacgtaagaa
    18001 aaaaataaag aatttatggt agaaataagg tagactgtat aatttgtccg tagtaaagta
    18061 aggaaacaag aataaatttc aatgaaggaa gagataagat gcgaaaagta gcacctattt
    18121 tgaagccaat cgctgtatta acaatctcga cgattgtcgc gactactaca ggtattgtga
    18181 atagttccag ctaccgtgca ccaagttcaa gttctagtac tgtagaagaa acaacaccaa
    18241 ctacttcagt ctcgacatct gaaccaacaa caacggaaag tttgaatgaa accactacga
    18301 caagtacatg ggacgataca acaactagtt cgtcgtatga tgagacatat acaactgaca
    18361 gctcaagcga aacaagtaat acggtaccag atggcactcc atcggattca accaatccaa
    18421 gtacaagtcc ggatacaagt gctagctccg aaacgatacc aagcagtacg actgtagatg
    18481 ataacacgca aaatgatgat gcagcacgta cagcacagca aaaccaagat tcaggaaata
    18541 cccaaacgta taaccagcaa gcaggtgatt ctcaatgaca tttttactag accttatcaa
    18601 cggggtgcat aaattccttg gctatttaga catcagtcct aaatatctta accgtggata
    18661 tacgatttta agtgtgattc ccacgcttta tttattgaga attgtttatg gactttggca
    18721 aaaccaaaac tatcttcaat ttttcttata tggacttgca tttctagtgc ttgtttattt
    18781 tactgtttta aatgtatttt attacttttt agataaaaac acgaaagccg atgtcactca
    18841 gctttttgtg aagtatttac ctgatgaagc gttcaacatc cagacagaag aatcgataga
    18901 taatggaaat tcgatcgata aagtgaatac acaagaggtc atggtttctt atgacgaaga
    18961 ttatcaattg aaattagccg agaatatgcg gtatttgatt ggaaatggtg agatcaaaac
    19021 aaatgaactc ggtcgcatgg atggatttct ggtcgataga aatacattgt atccttatta
    19081 ctatgtcaag aaaacaggag agaaagagta tgttctaaat attggacgaa gctactcgga
    19141 catggaaaaa gtagggacca ttcgacttga ctcaacagaa gacacattgg aacctgtcgg
    19201 attgttcatc gtcggcggtg attttgtcaa agacgggtta cgctatcatg agccttatcg
    19261 tctgaaattg attgcaaaaa aggcacagtc gcaggaagaa ccagtggttt atagtcgtag
    19321 ccaaagaaga agacattcat aatgtgatga aacgccgtgt atcagtggtt gcgataaaaa
    19381 cgtttattcg gaataagggg gatgtgacac gtcttttgtc acgttccttt tttatataac
    19441 gagaaagata agacgaacac atcacttatt ctttcctttc atttataaac attgtagaat
    19501 aaagaaagta caacgaagta ttgtgaaaaa acgtaataaa taacgaggaa aatacaagta
    19561 ggaggtaagc tgtgaaagaa tttttgaaaa attgggggat tttgatcctt gtattagcgg
    19621 ctattttatt agctagagtg tacgtgttta caccagttac tgtaaacggg cattcgatgg
    19681 accctacatt aagtgatgga caacgattga tttcatctaa gatctcaaac tatgaacgaa
    19741 tggatattat tactacaaag gaaccggggg atgaagaacg gatgattgtt aaacggatca
    19801 tcggtatgcc tggagataca gtcaagatgg aaaatgacca gttgaccatt aatggcaaaa
    19861 agtatgatga accttatctg gatgagttca aaaaagaatt ttcagaagat aagttacaag
    19921 gagaatatgc ttatagctca ggattccaag cacaagcaga aagttcatcc acctttacga
    19981 gtgactttga atatactgtg ccaaaaggaa agtatcttgt tctaggcgat aaccgcttga
    20041 tttcaaaaga cagccgaatg tttgggttag tcgacaaaga tatgatccaa ggaaaagttg
    20101 ttttccgtta ttggcctctt tcagaaatta aaatcatgtg atgaaaaaat aaaaagaagc
    20161 tgtgacatgt tttttgtcac agcttctttt tccgaaaatt ccttcttatt tcttggagtt
    20221 gctcactttt tttgcaatct cattttaagc tttttggctg atggataaga aaagcgaagc
    20281 gtttttgttc ataaatagct tttccccacg atggaaggta tgactagtag cagagacaaa
    20341 gcccgttttt tccaatcgat tattcaattc ttcttggcta aagccattat ggactttcgg
    20401 atgactaatt tgttcattct tgtcaaagtc tacgatgatc aattgaccgc caggagctaa
    20461 aatttcataa agttttgtaa gaatattctc cgtatcagga atgtgtaaaa gtacaagtga
    20521 tagaaggatg agattagctt ggacagcagg aaattcgaca gaagcatcag cgtggatcgt
    20581 tcggacattt ttcagatctg ctgcttggat cttttcttct gccattttca gcatagtttc
    20641 tgaagcatcg gcaatgatca gttctttgaa tctttccgca agcggtaaac taacaagccc
    20701 agttccgcct ccgtaatcca gcaggacttt ttggtggctg tcgcgcggta gataagtagt
    20761 tagttcttcg cgaataatct tggctaatgc ttgacggtca ggggaatcat aatggttagc
    20821 gatggaatca aaaatatttt ttgtcatcaa atagctcctt acgtaaaact ttgaaatttt
    20881 aacttattat agcacgtgaa aagaaagaaa gcatgctata ctcaattcaa aaagggagaa
    20941 tctagaaaga atatgccgat atcgtttgta aaagatagag aggaaaaagg aaagtgtgtc
    21001 cgagaaatat tactagactt gcctgagtgg tttgggcttc ctgaatcaac ggaaaaatat
    21061 atcgaggaat caagtaaact acctttatgg tgtgaaaaaa gaaaagaaga atatttaggc
    21121 tttatcactc tttcacagac aagtgaagat actgccgaaa tctattctat tgtatgggaa
    21181 taaaaaaaga ctatcatcac caaggattag gaacaaaact ttttaaagaa gcagaaggtt
    21241 acgcagcaaa acattacaaa tatttgcaag tgaagactgt agacgagggg cactactcta
    21301 tatatgatca aactatttgt ttctacgaat cattaggatt ttcaaggttg gaagtttttc
    21361 caaatttatg ggatgaatgg aacccatgtt tagttcttgt caaaaaattg gaacaaaagt
    21421 gagcaataga tttaatacta atctgtgctt gatgaaaatt ctcactgtaa aaaatgctga
    21481 aaatcaagta taataccata ggaacaaact ggaaggtgaa aaagagcatg gcacaactat
    21541 tttttaagta tggcgcaatg aatagcggca agacgatcga gatcctaaaa gtcgcacata
    21601 attatgaaga acaaaataag ccagttgtct taatgacaag cggtattgat acaagagatg
    21661 gtttaggcgt tgtttcaagc cgaatcggac tgaaacgtaa tgcgatgccg atatttgaag
    21721 aaacagacgt atttgacgta attcagaaaa tggaaagcaa accctattgt gtgctgatcg
    21781 atgaagcgca attcttaaaa aagaaacatg tcattgcttt tacgagaatt gtggacgaat
    21841 tggatattcc ggttatggca tttggtttga aaaatgattt tcgaaacgag ttatttgaag
    21901 gatcaaaata tttattgctt tatgcagaca aaatcgaaga aatgaaaaca atctgttggt
    21961 tctgccacaa aaaagcaatc atgaatttac attatattga tggaaaccct gtttatgaag
    22021 gcgatcaagt gcaaattggc ggaaatgaag cttattaccc cgtttgtcga aaacattact
    22081 ttcacccatc aatgattaca gaaggagaat aagcatgtac gatcaattac aagcaattga
    22141 agaccgttat gaagagcttg gagaactttt aagtgaccca gaagtgattt ctgatacaaa
    22201 gcgttttatg cagctttcaa aagaagaagc aaacacgcgc gaaacagtcg aagtttatcg
    22261 tcgttataaa gaagtagtac aaggaatcac cgatacagaa gaactgcttg gtgaaaaact
    22321 ggatgatgaa atggctgaac tagcaaaaga agaattatct gaattaaaaa aagaaaaaga
    22381 agtcttagaa gaaaaaatca agatcttgct tttaccaaaa gatccaaatg acgataaaaa
    22441 tatcatcatg gaaatccgtg gagcagcagg cggagacgaa gcagcattat tcgctggcga
    22501 tcttttcaat atgtaccaaa agtatgctga agcacaaggc tggaaaacag aagtgatgga
    22561 agctaacgtt accggaatcg gtggatataa agaagtgatc atgatgatca ccggagacaa
    22621 cgtatattcg aaattaaaat atgaaagtgg tgcgcaccgc gtccagcgtg ttccttcaac
    22681 tgaatcgcaa gggcgtatcc atacttccac cgcaacagtt gtcgtgatgc cagaagctga
    22741 agaagtagag atcgatattg aagacaaaga tatccgtaca gatatctatc atgcttcagg
    22801 tgctggcgga cagcacgtca ataaaacagc atctgctgta cgtttgactc acttaccaac
    22861 agggatcgtt gttgctatgc aagatgaacg ttctcaaatc aagaaccgtg aaaaagcaat
    22921 gaaaatcttg cgtgctcgtg tgtacgataa gatccagcaa gaagcacaaa gcgagtatga
    22981 tgccaatcga aaatcagcag tcggaactgg ggatcgttca gaacggatcc ggacgtataa
    23041 tttcccgcaa aatcgtgtga ctgatcatcg tatcggatta actatccaaa aattagatca
    23101 gattttagca gggaaattag atgagatcgt tgatgcattg atcatgtatg atcaaacatc
    23161 aaaactggaa gctatgcaaa atgggtaaaa tgacctatcg agaagtcctg tcacgggctt
    23221 cttctttttt agaggatcat gggaaagaag catatagtat ccaattttta tttttggaac
    23281 gaaaacaatg gaaaaaactt gattggctcc ttcatatgaa cgaagagatt tcagaagaag
    23341 aacagcggtt gatcgaaacc gatctgcgac ttctgcttgc agatcatcca cctcagtatt
    23401 tgctaggata tgcagatttt tatgatcatc ggttaaaggt aactgaagca acactgatcc
    23461 ctcgaccaga aacagaggag ttagtcgaat ggtgtttaga cgagacgcct ggtgtaccgt
    23521 tagaagtgat agatattggg acagggactg gagcaattgc tattagtttg aaagcagcaa
    23581 gaaaaaattg gcatatatct gctgtggatc tttcagaaga agcattagaa gtagcgaaag
    23641 aaaatgctca gaaagaagga acgaaaatca gtttttatca tggagataca ttaggaccag
    23701 taatggatca gcaatttgat gtgattattt ctaatccgcc gtacatcagt cggaatgaat
    23761 gggaactgat ggatgaaagt gtccggtcat tcgaacctaa aatggccctt tttgctgaga
    23821 acgatggatt agctatttat gaaaaaatag cagttgaagc accaagcgta ctaaaatcaa
    23881 atggaaagat ttttttagaa atcggatttc gccaaggtga agcagtaaaa aaaatatttc
    23941 agcaagcatt tccagataaa aaagtggcga taaaaaagga tctttttggc aatgaacgaa
    24001 tgatccaagt ttatggttga agttgtggat gaaaaatttt ttatttgttt ttttattata
    24061 aaaaaacact tatttgacaa ggatgtaaac gactttttgg tttatcaaca agtttatcca
    24121 ctattgacaa atagattatc cacaaacagt ggaaaacttg aggataaact cagacaaaat
    24181 aagaatgtat gaaaaagggg ttgtggataa actgtggaaa cgaaacgata tgatgaaaca
    24241 gaattgcaag aggcagccga agcactgaaa gaaggagaac ttgttgcttt tcctaccgaa
    24301 acagtatacg gattgggcgc caacgctctt ttaccaaacg ctgtcaagaa agtgttttct
    24361 gtaaaaggga gaccgcaaga taatccatta atcgtccatg tcgcctcttt tgaacaagta
    24421 aaagagtatg tggacaactt tcatccggca gcagagaaga ttgtgaagaa cttctggcct
    24481 ggaccattaa ctttgatatt taagatcaaa aaagacactt tgccctcagt tgtgacggga
    24541 ggattatcca cagctgcatt tcgtataccg gataacaaaa aaacactaga agtaatcgaa
    24601 ctttccggtg ttccgcttgt cggtccaagt gcaaatacgt cgggaaaacc aagtccaacg
    24661 actgcagatc atgtatatca tgatctacaa ggaaaaatta ccgggattat tgatgatgga
    24721 gctacgcgaa ttggtgtaga atccactgtt cttgatttaa gtgatccaac agccatgcct
    24781 atgattttac gaccaggtgc agtaacgaaa gaacaaatcg aagcagtcat cgaatctcct
    24841 gttgctattg atcagcatct tgtaaaagaa aatgaaacac caaaagcccc tgggatgaaa
    24901 tacaaacact actcacccga tacacgtgtt ctgatggtca gagatggcga ttggtcaact
    24961 gcagttcaat gggcaaaaaa taagaagatc cgtgcaggtg tcatagcaag ccccgaaatt
    25021 gcggatcaag tgcggacaga tactgcagct gtttatatgt ataacgacaa ctcagtagaa
    25081 gcggcggcta aaggattgtt tgcaggtcta agaggattag atgaaccaac acttggatta
    25141 gatttgattt ttgtacaagt gtatccagaa actggattgg ggaatgctta catgaatcgc
    25201 ttgaaaaaag cagcaggaca aaattatttt gaaaaatgat caattggtta ttgtcgcatt
    25261 cttttagtat gatacaatgc tatcaagtaa atcttctatc agtgaacgaa aaactaagcg
    25321 taactaatcg taatagaaga gatacgaata tttggcaatt tgtttttgat gggaaaaata
    25381 aaattgtagg aaaataaaat tgtaggaaaa taaaaaggag ctgttgtttg tggtagatta
    25441 caaaacgttt gaccctgatc tttgggctgc gatcgccaaa gaagaagaaa gacaagaaca
    25501 taatttggag ctgattgcct ctgaaaactt tgtctccgaa gcagtgatgg ctgcacaagg
    25561 aagtatcttg acaaataaat acgcagaagg ttatccaggg catcgctatt atggtgggtg
    25621 tgaatttgtc gatatcgttg aaaatttagc gattgaccgt gcaaaagaat tattcggagc
    25681 gaaatttgcg aacgtccagc ctcactccgg ctctcaagcc aacacggctg cttatctcgc
    25741 tttggtagaa ccaggggaca cgatcttagg aatggatctt tccgcaggcg gacatctaac
    25801 acatggatca ccagtcaact tcagtggcaa aacctatcac tttgttgctt acggtgttga
    25861 tccgacaaca gaagttattg attataatgt ggtacgtatt ttagctagaa aacatcaacc
    25921 aaaattgatc gtagcaggtg ccagtgctta cggacgaacg atcgactttg cgaaattcag
    25981 agaaatcgca gatgaagtag gcgcaaaact gatggtagat atggcacata tcgcaggatt
    26041 agtagcagca ggacttcatc ctaatccagt tccttatgca gatatcacaa cgactaccac
    26101 tcataaaaca ttgcgtggcc ctcgaggcgg catgattttg acaaacgatg aagcattagc
    26161 aaaaaaaata aatagtgcgg tatttcctgg tattcaaggc ggccctctag agcatgtgat
    26221 tgcgggtaaa gcagtagcat tcaaagaagc attagatcct gcatttaaag aatatagtga
    26281 acaaatcatc gccaatgcaa aagcaatggt caaagttttc aatcaagcaa ttggaaccag
    26341 agtcatttca ggggcgacag ataatcatct gatgttgatc gatgttcgcg agttaggtat
    26401 aaatggaaaa gaagcagaga gcattttgga cagtgtgaat atcactgtaa ataaaaactc
    26461 gattcctttt gaaacactaa gtccattcaa aacaagcggt attcgaatcg gcacgccagc
    26521 aatcactact cgcgggttca aagaagaaga tgctgtaaaa gtggctgaac tagttgtcaa
    26581 agcattacaa gcaaaagatg ataacgctca actagacgaa gtcaaaacag gtgtacgcga
    26641 attaactgaa aaatttccgt tgcataaaaa ataaaaattt aaattccatt agtaaacaga
    26701 aaagttcaat gttgggcttt tctgtttatt ttttgggaaa acggattctt tgacaatatc
    26761 ggtgatgctc tctagtatat gaggtgtaat tttagtcttc acactaaatc tcagggagga
    26821 aaagtcatga ctgatcgtat ctcgttagaa aaggaagcag tagcattcag tgatgcaaat
    26881 gcgcctcatc cgcgtatcta tgaactagca ccggaagaag gaagagtttt actagaagaa
    26941 gtacaagaat cacctgtgaa caaatattcc gtcgatattg aagacttgat ggtggatact
    27001 ggcgaatggg gaaaactcaa tattcgtctc gtacgaccag aagggaatat ggataaatta
    27061 ccagttattt tttatattca tggagcaggc tgggtttttg gcagtgctca aacccatgat
    27121 aaattgatca gagaacttgc ggttcgtacg aattccgtcg ttgtctttcc agaatatagt
    27181 cgatcaccag aagcaaaata tccaacagca attgaacaaa attatgctgt gttgcaacag
    27241 ctgaataaat tagccaagtc aaaaaatctg gatctcgaga atctaatcgt tgcaggagat
    27301 tcagtaggtg gtaacatggc tacagtcatg acgatcttaa caaaagaacg aaagggcctc
    27361 ccaatatcca agcaattgct gtattatcca gtcacaaacg ctgattttga tacagattcc
    27421 tatcatgaat tcggagaaaa ctattttttg acaagagaag gaatgatgtg gttttgggac
    27481 caatatacga cgaatgaaga agagcgtgct gaaatcacag cttcgccttt gagagcaaat
    27541 ataaatgaac taaatgatct tccttcagca atgattttaa cgggagaagc ggatgtatta
    27601 agagatgaag gtgaggctta tgcgcgcaaa cttcgtgatg caggggttcc tgttacacaa
    27661 gtacgcttcc aaggaatcat ccatgatttt gtcatggtaa atgctatgga tcagacaaag
    27721 gcaacgcgtg cagcaatggc attatctact tcttggatca agaaaaaaga ataagctttg
    27781 cttgtccgca ggaaaacttc ttttcaaaaa cggattttct taggaaaaat ggcgagtttg
    27841 actagtcaat cacttgattt tgttttacaa ttaaaaagaa acgacagaac aaaggagagc
    27901 ttaaaaaatg ggcaaatttc aagtgatcga tcatccattg attcaacaca aactaactat
    27961 tattcgggac aaaaactgcg gaacaaaggt ttttcgtgaa gtagtcgatg aaatcgctat
    28021 gttgatggca tatgaagtat cacgggatat gccattggaa gacatcgtga tcgaaacacc
    28081 gatcactgaa acaacacaaa aaacgttgtc tggtaaaaaa gtagcgatta ttccaatctt
    28141 gcgcgcaggt attggaatgg ttgacggtat cttggaattg attccagctg ctaaagtggg
    28201 acatatcggt ctttatcgag atgaagaaac attggaacca catgaatatt tcgtcaaact
    28261 tccagaagac attgatgcac gtcagttgtt tgtagtagat ccaatgttgg caacaggcgg
    28321 atcagcaatc atggcaatcg atgcattgaa agaacgtggc gcgtcgaaca ttaaatttgt
    28381 ttgtttggta gcagcacctg agggagtaaa agctttgcaa gaagcgcatc ctgatattga
    28441 catctataca gcttctctag atgaaaaatt gaatgaacat ggttatatcg ttcctggact
    28501 aggggatgcg ggtgaccgtt tgtttggtac aaaataagta aatacgaaaa agggtgtgac
    28561 acaacttttg tcacatcctt ttttggtcat ttttaacgtt tgatgcagaa cagctttttc
    28621 acagctttcg tttgcatata aaaaaaccat cccaaaaatg cagagatggt caaattcaaa
    28681 tcttttttag ttctttgtag ttaacgatta ctacaccctt actttgcgtc atcaagttcg
    28741 cattttctgc agtttcatca atctcaacaa cagcggaatt tttcaattgc tttgaaatcg
    28801 ttccagtttg cgacttgccg tgaattgtga acattacgtg agtattgatc ttgaattttt
    28861 caccaatttc cgatggtacg atagttgtat caaattttcc aaaagttgcc attttcataa
    28921 aacctgcctt tcacatcaat tataccacgt tttatgaaaa atatcatgaa attaaactaa
    28981 ttttccatcg gactctttac aatcagccaa atagagtata taatttcttt tggataaggc
    29041 cagcttagct cagttggtag agcaacgcac tcgtaacgcg taggtcgtag gttcgatccc
    29101 tatagctggc attaaaagag aagacagata tatcaaggat ttttaatcca ttgatacatc
    29161 tgtctttttg tttaaaggaa ataaagtatg ctttcttagc aatagagcat gtattatttc
    29221 tataaaaatt taggagaata aaacgactag caatatagct gtgaaatcaa atatcacatg
    29281 aactgcccaa cttgtaatga tcgagttgct tttaatggca gcttggttga ataacaatct
    29341 tgccacccct tgtatcaaaa ggatgtgtgc tacagtgtgt aaaatatttc cattatcata
    29401 agtactgtaa tggataaatc caaagatgat tgctgacaag aaactagcga caggaatgct
    29461 gaacttacta gaaaaaatag cgagaaaata cactgagaac aattcttctc caagtatcat
    29521 gataggcaaa ataataaata ataacgggct gaaagcctca tttattggat tgcctgttaa
    29581 accccatttc aaaatttgtt gcaaaaatac cgagactgcg gcactgatta ctacattgat
    29641 aaaaaaatat ttggctatat ttttgacagg agcgacaggt ttcgagaaca tttttttcgt
    29701 atctttttta ccaaaaacaa gtgtaaatgc tataaatcca gtgatagtta aagaaaaaat
    29761 gctaataatg gatggcgcaa atagcaaccc taacaaaccg agtacaacga caatagtgcc
    29821 caatatccct tttttaagtt ctattttttt tcctaaaatg ttattcatga taataactcc
    29881 tactattcag tttttgaaga tagagtcagt atacatgaag agttaaaata ggcaa
//
LOCUS       DADVXU010000022        29657 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.024, whole genome shotgun sequence.
ACCESSION   DADVXU010000022 DADVXU010000000
VERSION     DADVXU010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 29657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.024"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            355..669
                     /locus_tag="KB953_000971"
     CDS             355..669
                     /locus_tag="KB953_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865964.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFQSFRNQIIRSGKT
                     VN"
     gene            complement(722..1495)
                     /locus_tag="KB953_000972"
     CDS             complement(722..1495)
                     /locus_tag="KB953_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865965.1"
                     /translation="MKKFAIILTCALLIGTISGCTTSDTSSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSETESTEKILPIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKVNIEKGALAQLYTEKVEQFFEAGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMKLKKIKSYDYPLNLTLNKGILSFVLAGYVDYEYTADYLETPLQHDNNDINFR
                     FIYNSKSKTWLVDQLSTINTGFHELPKPDNETALVIKTF"
     gene            1600..1845
                     /locus_tag="KB953_000973"
     CDS             1600..1845
                     /locus_tag="KB953_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6865966.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRVRLEKGIGQTELIRDLQLRKIA
                     ITRETLVKIEGGRQHIKLDQLKAIKDILQVSYEDLLQ"
     gene            2001..2852
                     /locus_tag="KB953_000974"
     CDS             2001..2852
                     /locus_tag="KB953_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HBB6865967.1"
                     /translation="MYKAYELDFTNADLNAFSSSSQSYTHVVNQIDHNQKSINKRIEN
                     LFESENDLVDYYSKDSKILDADEIIKDWFPTIKADIFISHSHADEKLAIRFASWLFEN
                     FGLTAFIDSSVWGYSSDLLKKIDQKYCYKEQTKTYDYDKRNVTTSHVHMMLSTALNNM
                     IDSTECLFFLNTPNSISLSNEITNEQKFTYSPWLYSELTTASIVEKKNPRLESNPQMS
                     TEDVRSIIKHYSSQENLQIKYSLNLSELTYLRKADLKKWALDAVECTKGSNTLDILYK
                     NTIFTNL"
     gene            2885..3559
                     /locus_tag="KB953_000975"
                     /pseudo
     CDS             2885..3559
                     /locus_tag="KB953_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287699.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
     gene            3816..4004
                     /locus_tag="KB953_000976"
                     /pseudo
     CDS             3816..4004
                     /locus_tag="KB953_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604486.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            4050..5015
                     /locus_tag="KB953_000977"
     CDS             4050..5015
                     /locus_tag="KB953_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6865968.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            5037..5249
                     /locus_tag="KB953_000978"
     CDS             5037..5249
                     /locus_tag="KB953_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HBB6865969.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            5324..5800
                     /locus_tag="KB953_000979"
     CDS             5324..5800
                     /locus_tag="KB953_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HBB6865970.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            5797..7605
                     /locus_tag="KB953_000980"
     CDS             5797..7605
                     /locus_tag="KB953_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HBB6865971.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            7682..7897
                     /locus_tag="KB953_000981"
     CDS             7682..7897
                     /locus_tag="KB953_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family protein"
                     /protein_id="HBB6865972.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            7989..8852
                     /locus_tag="KB953_000982"
     CDS             7989..8852
                     /locus_tag="KB953_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865973.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            8889..9425
                     /locus_tag="KB953_000983"
     CDS             8889..9425
                     /locus_tag="KB953_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HBB6865974.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            9439..9861
                     /locus_tag="KB953_000984"
     CDS             9439..9861
                     /locus_tag="KB953_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HBB6865975.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            9791..12190
                     /locus_tag="KB953_000985"
     CDS             9791..12190
                     /locus_tag="KB953_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HBB6865976.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            12222..14213
                     /locus_tag="KB953_000986"
     CDS             12222..14213
                     /locus_tag="KB953_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBB6865977.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            14236..14487
                     /locus_tag="KB953_000987"
     CDS             14236..14487
                     /locus_tag="KB953_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HBB6865978.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            14477..15706
                     /locus_tag="KB953_000988"
     CDS             14477..15706
                     /locus_tag="KB953_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HBB6865979.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            15703..17784
                     /locus_tag="KB953_000989"
     CDS             15703..17784
                     /locus_tag="KB953_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HBB6865980.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            17933..21853
                     /locus_tag="KB953_000990"
     CDS             17933..21853
                     /locus_tag="KB953_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HBB6865981.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKTD
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLIERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            21854..22798
                     /locus_tag="KB953_000991"
     CDS             21854..22798
                     /locus_tag="KB953_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865982.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            22802..23005
                     /locus_tag="KB953_000992"
     CDS             22802..23005
                     /locus_tag="KB953_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HBB6865983.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(23305..23541)
                     /locus_tag="KB953_000993"
     CDS             complement(23305..23541)
                     /locus_tag="KB953_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6865984.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(23724..24110)
                     /locus_tag="KB953_000994"
     CDS             complement(23724..24110)
                     /locus_tag="KB953_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HBB6865985.1"
                     /translation="MDARAMSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECI
                     CVQSISYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAA
                     CSKRMRRRKEAERQRKRYWKPTHLEL"
     gene            complement(24092..25420)
                     /locus_tag="KB953_000995"
     CDS             complement(24092..25420)
                     /locus_tag="KB953_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBB6865986.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(25381..25710)
                     /gene="mobC"
                     /locus_tag="KB953_000996"
     CDS             complement(25381..25710)
                     /gene="mobC"
                     /locus_tag="KB953_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HBB6865987.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(25968..26338)
                     /locus_tag="KB953_000997"
                     /pseudo
     CDS             complement(25968..26338)
                     /locus_tag="KB953_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            26483..26668
                     /locus_tag="KB953_000998"
     CDS             26483..26668
                     /locus_tag="KB953_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBB6865988.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            26826..26993
                     /locus_tag="KB953_000999"
     CDS             26826..26993
                     /locus_tag="KB953_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865989.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            27180..27398
                     /locus_tag="KB953_001000"
     CDS             27180..27398
                     /locus_tag="KB953_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6865990.1"
                     /translation="MRQERPEGVHTGPACVKSHTARCIRKKEAAHFAEAWGVPNKQNE
                     NAAGGQVVDQNIRKGYSDPLLASIIGMF"
     gene            27611..28270
                     /gene="vanR"
                     /locus_tag="KB953_001001"
     CDS             27611..28270
                     /gene="vanR"
                     /locus_tag="KB953_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HBB6865991.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            28273..29634
                     /gene="vanS"
                     /locus_tag="KB953_001002"
     CDS             28273..29634
                     /gene="vanS"
                     /locus_tag="KB953_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HBB6865992.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLT
                     STL"
BASE COUNT         7759 a         7430 c         7765 g         6703 t
ORIGIN      
        1 aagttgaagg agaaaaaata tttaaaatat acttcccatt tcagtgggaa gtaaaattaa
       61 tcttgttgtt tctactagta atgtatcttt ttggaaacta tgtaacgatg taaacaagat
      121 ttttttcaga agaaaaaacg ataaaagttg tgataaacga tttaaaaaat aggagaaaat
      181 atatcaagcg catggtatga aacgcacatt taaaaagatt cgactaacta aaaaaataag
      241 tttacaaagc attgttgtgt attacaagtg atctatacac tgaaaaatga tagagaaaat
      301 ctgatacgtc cgcaacaatg gacaattgaa aatgaaagtg aggaaattgg aagaatggct
      361 gatgaaaata aagttgttcc gcttgaaaca ctagtgataa ataaggaaga gtttgagcaa
      421 ctttttcttg atttttttgt tcaatatgac aggatttatg attttagttc gtcactagat
      481 ttatttgaat tagaggcaga aataatagaa aacagtcgag aggagaatgg taaaatcatc
      541 atttcagtag aaaaattcaa tgaattattc tcagaaacat ttgaaataga tacagattat
      601 tatcaaccac aagaagtttt ccaatcattt agaaatcaga ttattcgctc aggtaagacc
      661 gtaaactgaa aaacaataaa agaaacgttg aaagaataat tattccttca acgtttcttt
      721 tttagaatgt tttaatgacc aaagcagttt cattgtcagg cttaggtaat tcatgaaatc
      781 ccgtatttat agtgctcaat tgatcaacta accatgtttt actttttgaa ttgtagataa
      841 atctaaaatt aatatcgtta ttatcgtgtt gaaggggcgt ctccaaataa tctgctgtat
      901 attcatagtc aacgtaacct gctaatacaa aagagagtat ccctttattt aatgttagat
      961 ttaatggata atcatacgac tttatttttt tcaacttcat attgtacata tcttcagcca
     1021 taaaagttga gttcgtgatt tcatcttggg tctcattaaa aaagttatcc gaataatcgc
     1081 ttatttttga aaagtcttta ttggcaccag cctcaaaaaa ttgttcaact ttttcagtat
     1141 aaagttgtgc taacgctcct ttttcaatat taactttaat attttcatgt ctatcaattt
     1201 gtgtgctgtt cagtaaggga agatcagctt ttagttttat atcgtcttga tttctttcaa
     1261 tttcaattgg caaaatcttt tcagtagatt cagtttctga taaaggaaca tctagttttt
     1321 tatcatgata ataagcttca aatccatctg gaaccggaaa aaaatgtatt gggtaattct
     1381 tttctgaggt tgttgtacta gtttccgtac ttgtagaact tgtgtcagaa gtggtacacc
     1441 cgcttatagt tccgatgagt aaagcgcaag ttagaatgat tgcaaatttc ttcaaaaaaa
     1501 ttcctcctta aaattgaata tggactaata ctagtcccat tgtagcatac cgaatcttta
     1561 agattaataa gaaaaaggac taattttagt ccaaaaggtg tgctattctt gaaagaggaa
     1621 aaaattattg ataaagaaaa tgtaatcggt agcaatatta gacgagtccg attagaaaaa
     1681 ggaattggtc aaaccgagct gattagagac ctacagcttc gtaaaattgc tataactagg
     1741 gaaacgttgg ttaagattga aggtggccgc caacacatta agttggatca attgaaagcc
     1801 atcaaagata ttctacaggt gtcttatgaa gatttgcttc aatgactact tgtagtcatt
     1861 tcgtttgaaa ttattatgtt acacccttat gtgtcacgaa gaaatactgg tataataatg
     1921 aatgggatat gtaacgatag ggtatactgt tatgttgcct aataaattgt ggatcaaaat
     1981 acaagaatgg agggatatcc ttgtataagg catatgaatt ggattttaca aatgctgact
     2041 taaatgcatt ctcctcaagc tcgcaatcat atacacatgt agtaaatcaa atagatcaca
     2101 accaaaaatc tattaacaag agaatagaaa atctatttga aagtgaaaat gatttagttg
     2161 attattattc taaggacagc aaaattctag atgcagacga aattattaaa gattggtttc
     2221 caactataaa agcagatatt tttatatctc actctcatgc tgatgaaaaa ttagctatac
     2281 gttttgcatc atggctattt gaaaattttg gtcttactgc atttattgat tcttcggttt
     2341 ggggatacag ttctgattta ttaaaaaaaa tagaccaaaa atattgctat aaggaacaaa
     2401 caaaaacata tgattatgat aaaagaaatg taacaactag tcatgttcat atgatgcttt
     2461 ctacagcttt aaataatatg attgatagta cagaatgtct gttttttctt aatacaccta
     2521 attcaattag tttgagcaat gaaatcacta atgaacagaa atttacgtat tcgccatggc
     2581 tatatagcga actgacgaca gcttcaatag tggaaaaaaa gaatccacga ttggaaagta
     2641 atcctcaaat gagcaccgag gatgttagaa gcattataaa gcactattct tcgcaagaaa
     2701 atctgcaaat aaaatattct ttaaatttaa gtgaattaac ttatttacga aaagctgatt
     2761 tgaaaaaatg ggctttagat gctgtggaat gcactaaagg aagtaataca ttagatattt
     2821 tatataaaaa caccattttc actaatctct aatcaattta ctgaatcgaa aggaaccggg
     2881 tagaatggat aagaaagttt taataaagag gttctctgaa gctattgaaa aaggaaatgc
     2941 agcaatattt gcaggtgcag gattatcgat gtcacaagga tatgtaagtt ggccggaact
     3001 acttaatgat cctgcaacag aaatcgggtt ggattctaaa aaagaaacag acttagttac
     3061 tctggctcaa tattataaaa atgaaaatgg tggatcaaga ggaattttga atcagatact
     3121 tatggataat tttggtgaag aattagaaat atctgaaaat catcgaatac tagcaagttt
     3181 acctattgaa acttattgga caactaatta tgatcatcta atagaaaaat caatccgaga
     3241 agcttataaa aaccctcagg ttaagaaaaa ttatactcag ttagcaacaa ctaatccaaa
     3301 tgtggataca attgtttata aaatgcatgg tgatatagat gatgtcagtt cgacagtaat
     3361 aacaagagat gattatgaaa aatatgatga ttcgtacgcg ttatttaaag aaactttaaa
     3421 aggagatttg ttaacaaaaa cttttctctt tttaggattt agttttacag accctaattt
     3481 agaaagaatt ttatcagata taagatgggt tttgagagaa aaccaaagac ctcattattg
     3541 tataatgaga aaaatattat agaaaaaatt ttaggttatc gctgaaagcc ccggaaatac
     3601 gggcatttcg caggatatga gaactcaatt tactaccaat ttactacttt cggattgagc
     3661 cgcatatatg ctgaaccata ctgctaatac ccgccacccc aaatacggca catcataaat
     3721 tgaatacgac accgtaaacg cggcgttcct ctttcccgtc atgggagaac aggacgccgc
     3781 ttttttatgc ccaaatagag aaaggaggcg acgccatgct atttacttcc ggccctgacc
     3841 gggcgtttga acggctgatg caggaaaagc ccggtttcga tcactacgac ggcggcgggg
     3901 cggacgcgcc ggaggattgc ggcacctgcc gattttaccg cccggattgg aaatatcaat
     3961 tctgccagtt tgcagagtgt ccctatcagc cgggcaagct gaccgactat gcagcggtca
     4021 aattcacagt gaaaggagcg aacaacgcta tggcagtttt ccgtgtggaa aagaacaaag
     4081 gctatacggt gatgtcaaat caccacctgc ggaataagaa cctgacctta aaggccaagg
     4141 gcctgctgtc gcagatgttg tccttgccgg aggattggga ctatacccta aaaggactgt
     4201 cctatatcaa ccgggaaagc atcgacgcga tcaggacagc ggtatgggag cttgaaaaag
     4261 ctggatatat ccggcgtgag cagggccgtg acgcaaaagg caaaatggcc gatatggtct
     4321 ataccatcta cgaacagccg gtattggaaa acccggtatt ggaaaatcca acatcggata
     4381 acccggtgtt ggaaaatccg acatcggata atccggcgtc ggaaaatcca acgcaattaa
     4441 ataaagaagt acaaagaact gacttatcaa aaaaagaaaa aataattaca gatggacaaa
     4501 gtacccattc cattcctatc ccatccccga cccctgcccc tatggagggc gaggcagcgg
     4561 ctacgccgcc ggaacggaaa aggacaggaa aggatgaggc tgtacggatt tatcgggaaa
     4621 tcattttgga gaacatcgag tatgcctatc tcattcagga caggagcatt gaccgtgaac
     4681 agcttgacga aatcgtagac ctgatgctgg aaaccgtctg caccgcccga aagacaatcc
     4741 gtgtcgccgg ggacgactac cctgccgagc tggtgaaatc caagtacatg aagctgaccg
     4801 gcgaacatat ccgctttgtc cttgactgtc tgcgggagaa cacaacgaaa gtccgcaata
     4861 tcaaacaata cctgcgggct atgctgttca acgcccccag caccatttcc aactactaca
     4921 cggcccttgt cgcgcacgac atggcacagc ccgattgggg aaagcctaaa tccggcctgc
     4981 cggactattc ctgcggcgag ggcgaaagcc tgtaacgaga aaaggaggac gaccctatga
     5041 agcagggcgc attgattttt gacgagcgaa cagaccgtta cgacatccgc tttgatctgg
     5101 ccgactatta cggcggcctg cattgcgggg agtgtttcga cgtgatgatc ggcggcaaat
     5161 ggaggcccac ccgcattgaa atggccgaaa actggtatct tgtcggcatc cgggccgacg
     5221 acctttccgg cctgcgggtg cggatttgac ctatatctac ctgcggcgtc tggtggttcc
     5281 gccttagacg ccgcttttga ctttctgaca aggagggatg cgattgcagg aagaagttac
     5341 tcaaaagacc attgccctgt ccatgaaaac gggcaagctc acggctcaag tgctgcaagc
     5401 ggcgctgaaa aagtacctgc aacaccgggc caagggcaaa aacacgctgc accacggcca
     5461 gcagagccta aagcagctta aaaagcatgg ggctgatctc tccaacattg agatcaccga
     5521 ggccaacatt ggagcgttca agccatgcgc caagaaatac ggcgtggatt ttaccttacg
     5581 aaaagataag accacccagc cgccccacta cgttgtgatt ttcaaggcca aggatgcaga
     5641 taaccttgat ctggcgtttc gggagtttac cgccaagacg ctttccaagg agcagcggcc
     5701 ctctatccgc aaggttttgt ccgctgtgaa gcaaaaggcg gcggcccaga ccaagcagcg
     5761 ggccaaggag aaaatcaagg aaagggggct ggaactgtga agcctgacgt aaagaaattg
     5821 ctgatcctga acctcccgta tctgattttt gtgtacctgt ttgcgaaatg cggggaggcg
     5881 taccggctgg ccgctggtgc tgacctgtcg gggaagctcc tgcattttgc ggatggtttt
     5941 gcggcggctt ttgccaaccc gctgccaagc ctgcacctgt ttgacctgtg tatcggtatc
     6001 gccggtgccg tgatcgtgcg gctggtggtg tactgcaaga gcaagaacgt gaagaaatac
     6061 cgcaaaggca tggagtatgg cagcgcccgc tgggggacgg ccaaagacat agcgccttac
     6121 atcgacccca agtttgaaaa taatatcctg ctcacccaaa cggaacggct taccatgacc
     6181 ggcaggccca aagaccccaa gacagcgcgg aataaaaacg tgctggtgat cggtggcagc
     6241 ggttcaggga aaacgagatt tttcgtgaaa cccaacctga tgcaatgctt tccgaccacg
     6301 gactatccga cttcgtttgt ggtgacagat ccgaaaggca ctttggttct ggaaaccggc
     6361 aggatgttcc agcaggcggg ctaccgcatt aaaatcctga ataccattaa cttttccaag
     6421 tccatgaagt acaacccctt tgtctacatc cattcggaaa aggacgtgct aaagctggta
     6481 aataccctga tttccaacac caagggcgag ggggaaaagt cagcggagga tttttgggtg
     6541 aagtcggaac ggttgtttta ttcggccctg atcggctata tctggtacga ggccccagcg
     6601 gaggaaatga acttctcaac gctgctggaa atgataaacg ccagtgaagc ccgcgaggac
     6661 gacccggagt ttcaaagccc cgtcgatctc atgtttgagc gtttggagca gaaagacccc
     6721 gaccattttg cggtgcgcca gtacaagaaa ttcctgctgt ccgcaggcaa gacccgttct
     6781 tccatcctga tttcatgcgg tgccagactt gcgccctttg acatccggga ggtacgcgag
     6841 ctgatggagg acgacgaaat ggagctggac accatcgggg acgaaaagac ggtgctgtat
     6901 ctcattatga gcgatacgga cacgaccttt aatttcattc tggctatgct ccaaagccag
     6961 ctaatcaacc tgctttgtga ccgggctgat gatgtgtacg gcgggcggct gcctgtccat
     7021 gtacgcctga ttttggatga atttgccaac attggccaaa tacccaattt cgataaactc
     7081 attgccacca ttcgaagccg tgaaatatcg gcctctatta ttttgcagag ccagtcgcag
     7141 ctaaaagcga tttacaagga cgccgccgag atcatttcgg ataactgcga tagtgtgctt
     7201 ttccttagtg ggcgcggcaa aaatgccaag gagatttccg atgcgctggg gaaagaaacg
     7261 attgacagtt tcaacaccag cgagaaccgg ggaagccaaa cgtcccacgg attgaactat
     7321 caaaaattag gaaaggagtt gatgtcccaa gacgaaattg ccgtgatgga cggcggcaag
     7381 tgtatcttgc agctacgggg tgtgcgcccg tttttatcgg agaaattcga tattaccaag
     7441 catccccggt acaaatacct tgccgacgcc gacaagaaga atacctttga cgtggacaga
     7501 tttttgacta tcacgcgccg gaaacggcag caagtggtaa cgcaggatga agtattcgac
     7561 ctgtatgaaa tcgacctgtc ggacgaggac gccgccgctg aataaacagc ggcttttttc
     7621 attccaacaa caaaatcaaa aaatcattat gagccggaaa ccggcagaaa gaggtaaatt
     7681 tatggcattt tttaattccg cagttactac tttgcagact atcgtgatcg gactgggcgg
     7741 cgctctgtgc gtatggggcg gcatcaatct gttggaggga tacgggcagg acaatccggg
     7801 ggcaaaatcg cagggtgtca agcagcttgt cgctggcggc ggcgtggccc tgatcggtgt
     7861 cactctggtt cccctgctgt ccgggctgct gggctgatcc tggcctttgt ccctgtccaa
     7921 acccttaact aatcgcctcc gcgtcctccc ggccagaccg ggagggcgcg ggaaaggagg
     7981 tgctacccgt gattagtgaa atcattgaag aatggataaa aggcatcctc atggacggta
     8041 tcacgggcaa cctgtccggg ctgttcgata cggtaaatgc caaagtcgga gaaatcgccg
     8101 ccgacgtggg acgaaccccg caggcatgga acagcggtat tttcaatatg ctgcacagcc
     8161 tgtcggaaac cgtgatcgtc cccatagccg gggccatact cgcccttgtc atgtgctatg
     8221 agcttatcca gatgataacg gaaaagaaca atatgcacga ctttgacagc tccatgttct
     8281 tccgctggat attcaaatcc gcgtttgcaa tcctgatcgt aacgaacaca tggaacatcg
     8341 tcatgggcgt attcgacgcc acccaaagcg tcgtcaatca aagcgccggt gtgattatcg
     8401 ggcagacatc cattgatttt aacaccctga taccagacct tgaaagccaa ttggaggcta
     8461 tgagcgtggg cgctcttttg gggctgtggt tccaaaccct tgttgtgggc ctcaccatga
     8521 atatcctttc catctgcatt ttccttgtga cctatggccg catggtggaa atctatgttg
     8581 tcacgtcgct ggggcctatc ccccttgcca caatgggcaa cgcggaatgg cgcggcatgg
     8641 gccaagggta tctgaaatcc ctgctggcgc tgggcttcca agccttttta atcatggttg
     8701 tggtgggtat ctacgcggtg ctgatacaga atatcggttc cgcttccgac atatccggcg
     8761 caatctgggg ctgtatgggc tatacggtgc tgctgtgctt ctgcctgttc aagaccggca
     8821 gtatatcaaa ggccgtattc ggggcgcatt gatcccaaaa tccaagcata cacaggagga
     8881 acttgtttat ttcaacgtac aacatcattt acgctgaccc gccgtggcgg tatgactgca
     8941 aaaacgtaca gggcgcagcg gaaaatcact attccaccat gagcattgac gagctgtgcg
     9001 ccctgccggt ggaacggctg gcgtccaaag actgcctgct gtttttgtgg gccacattcc
     9061 ccatgctgcc ggaagctctg cggttaatca aagcgtgggg cttttcattc aagaccgtgg
     9121 cttttgtatg gctgaaacag aaccgtaaaa gccttacatg gttttacggg ctgggccgct
     9181 ggacaagggg aaacgcggaa atctgcctgc tggcaacaca cggcaagccg aagcgtcgtt
     9241 ctgcctctgt ccatcagttt attatcagcc cgattgaaca acacagcaaa aagccggatg
     9301 tgacccgtga aaaaatcgta gagctggcgg gcgatttgcc ccgcgtggag ctgtttgcca
     9361 gacagaaaac ccccggctgg gacgtgtggg ggaacgaggt ggaatgtgac cttatcctga
     9421 cgtgaaagga ggtgccaaat ggcttatgta accgtcccca aggacttaac ccatgtgaaa
     9481 agcaaggttg tgtttgggct taccaaacgg caactgatct gtttttccgg ggcgctggtg
     9541 atcggcgtcc ccttatattt tctcacccgt gactatctta ccaacagcgc ggcggccctg
     9601 ctcatggtat ttgccatgct gccgggcttt ttgcttgcca tgtacgagcg tcacggccag
     9661 ccccttgaag tggtgattat gcagatcatc cagtgctgct tcatccagcc aaaggagcgt
     9721 ccctatcaga ccgacaacac ctacaccgcc cttgtgcggc aataccagat ggaacaggag
     9781 gtaaaggcca ttgtccaaaa aggaaatccc aagagaaacc gaaaaaacaa agctgacccg
     9841 cgcccagcga aaagaaattg acgcggtaat ccgtaaatat aagggcgacg gcaggcccca
     9901 cacggcgcag cagagcatcc cctacgaggt catgtacccg gacggcgtgt gccgggtatc
     9961 gcccggcgtc ttttccaagt gcattgagtt tgcggacatc agctatcagc ttgcccagcc
    10021 ggacacgcag acggctatct ttgaaaaact gtgcgacctg tataactatg tggacgcctc
    10081 cattcatatc cagttttctt tcctcaatcg gaaagttgac ccggtgcagt acgccaagag
    10141 ctttgagatc gcgccgcagg gggacgactt cgacgacatc cgggccgagt acaccggcat
    10201 tctgcaaaag cagcttgcaa acggcaacaa cggcatggta aagaccaaat acctgacatt
    10261 caccatcgag gccgaaagcg tgaaagcggc gcgagccaga ttgaagcgca tcggctttga
    10321 cctgttaggc tatttcaaga gcatgggggc cgtagcccat gtgatggacg gctgggaacg
    10381 gctgaacctg ctgcatggcg tgtaccaccc ggacggcgag atattcaatt ttgactggaa
    10441 gtggctggcc ccgtccggcc tctccaccaa ggattttatt gccccgtcct ccctgtgctt
    10501 cggcaatgca aagacattcg gcatgggcgg caaatacggc gcggtaagtt tcctgcaaat
    10561 cctgtccccg gagctgtcgg acgatatgct ggccgacttc ctcaatacgg aaagcggtgt
    10621 tttagtcaat ctccatgtgc aggccattga acagaccaag gcgatcaaga ccatcaagcg
    10681 caaaattacc gaccttgacg ctatgaagat tgcggagcag aaaaaggccg tccgcagcgg
    10741 ttacgatatg gacattcttc ccagcgacct tgccacctac ggcgaggacg ccaagaagct
    10801 gctgacaaag ttgcagaccc gcaatgaacg gctgttccag ttgacgttcc ttgtcctcaa
    10861 cgtggccgac accaagcaaa agctgaataa cgacgtgttc caagccgcag gcgtggccca
    10921 aaagcataac tgtccccttg tccggctgga ctaccagcag gaacagggcc ttgcaagcag
    10981 cctgccgctg ggcgtcaatc agatcaagat acagcgcagc cttacgactt ccagcgtggc
    11041 cgtgttcgtc ccctttgtga cgcaggagct ttttcagggc ggcgcggcca tgtattacgg
    11101 catcaacgcc aagtcccgca acatgattat gctggacagg aagcaggccc gttgccccaa
    11161 tgccttgaag ctcggcaccc ccggcagcgg caaatccatg agctgtaaat ccgagatcgt
    11221 cagtgtgttc ttaacgacgc ctgacgatat ttttatttca gacccggagg ccgaatatta
    11281 cccccttgtc aaacggctcc acggacaggt aatccggctg tcgcctacca gcaaggactt
    11341 tgtgaacccg ctggacatca acctgaatta ttcggaggac gataacccgc tggccctgaa
    11401 atccgacttt gtgctgtcgt tctgtgagct tgtcatgggc ggcaagaacg ggctggaagc
    11461 cattgaaaag accgtgatag accgggctgt gcgcgtgatc taccggccct atctggcaga
    11521 cccccgcccg gagaatatgc cgatcctgtc cgacctccac aaagcgttgt tagatcagca
    11581 tgtcccggag gctgaccggg tggcgcaggc ccttgacctg tatgtgtccg gctccctgaa
    11641 cgtatttaac caccgtacca acgtggacat cggcaaccgg cttgtgtcct ttgacattaa
    11701 ggagctgggc aagcagctca aaaagctggg gatgctcatt gtccaagacc agatatgggg
    11761 gcgcgtcacg gccaaccgaa gccaaggaaa ggccacatgg tattttgccg acgagttcca
    11821 tttgcttttg aaagaggaac agacggcggc ctattctgcg gagatttgga agcggtttag
    11881 gaaatggggc ggcattccaa caggtgccac ccagaacgtc aaagacctgc tttcctcccc
    11941 ggaaattgaa aatatccttg aaaacagcga cttcattacg ctgctcaatc aggcgtccgg
    12001 ggatagaaaa atccttgcgg aacgcttgaa tttgtccacc gaacagcaga agtacatcga
    12061 caattccgaa cccggcgagg gccttttgat ctttgaaaac gtggtgctgc cctttaccaa
    12121 ccccatcccg cacaacaccc agctttacaa gattatgacg acccgcttaa acgaggtggc
    12181 gggagtatga cccggcgacg ccacaaagga ggtgaaccac catgaaagag ctgaaagcca
    12241 aggcgaaagt gacgcaggcc atgacccgcg acgggctgac gctggaaaat caggccaccg
    12301 gcgaggtgga gaacatttcc agccgggagg ccgaacagga tttgtccgcg ccggggccgg
    12361 gcggcaccgc cgataagctg ctggaacggg ccgacgccat ccatgacggc cataaggcca
    12421 agaaagccgc gaagcaggcc ggggctgtgg ccgccgaggg ggacgctgcc ctgcaccggc
    12481 catccgcccg gctccaactg tcggaggaag aacgggccga cccggattta cagccctata
    12541 tccgaaaatc ggacaaacgg gccgataagc tggataaggc ccgcgccgcc atccccaaga
    12601 agcgcgtccc ggtcaaggaa accgtctttg acgaggcaag cggcaaagca aagaccgtcc
    12661 tgcactttga caaacaggac aaggggcctc cctccctgaa acccaatccg gcaggccgtc
    12721 ctgtttccga ggtttttcta ttcggccacg ggaaaatcca tgaagtcgag catgaaaacg
    12781 tgggtgtgga gggcggccat aagggggagg aacttgccga gcggcaggcc ggaaaagcca
    12841 tccgcagcac catccggcac cacaggctga aaccctactg ggacgcggaa aaggccgagc
    12901 gcaggctatt gtctgccaac gcggagtatt tctaccagaa atctttgcgg gataacccgg
    12961 agatcgcaca ggcggccagc aaccccattt cccggttctg gcagaaacag cagatcaagc
    13021 gccagtacgc caaggccgcc cggacagcgg gccagaccgc aggcgcggcg gcacagggcg
    13081 cggccaagac agccggggcc tccacgaaag cggccaaggc cgcagccaaa gaaagtgaac
    13141 gggcggcctc ctttgtggcc cgccactgga aaggggcgct ggccgtcctt gtgttggggc
    13201 ttttgatgct gcttatcatc ggcggcttac agtcctgcac cgccatgttc ggcagcgccg
    13261 gaagcggcat cacggcgtcc tcttactttt ccgaggatag cgatatgttg gccgccgagg
    13321 acgcctacgc ggagctggaa gccgagctgc aagactatct ggacaactac gaggccaccc
    13381 atgactatga cgaataccat tttgaccttg acgagatcag ccatgacccc tatgtgctga
    13441 tttccattct gtcggcgctc catgacggga tatttaccat cggggaggta cagggtgatt
    13501 tgtccatgct ctttgacaag caatatatcc tgacagaaaa cgtcgtggta gaaacccgtt
    13561 accgcaccga aaccgataca tggacggacg cggagggcaa cacccataca gacacctacc
    13621 aagtgccgta tgactattac atctgctacg tcacgctgga aaacttcgac ctgtcccatg
    13681 tgccagtcta tatcatggac gaggaaacgc taagtttcta ttccctgtat atgtccacgc
    13741 taggaaaccg gcctgatctg ttttccggca gccagtaccc caacgcctcc accctgacgc
    13801 cgccgaccta ctacgacgta cccccggagg ctttggagga tgccaccttt gccgccatgc
    13861 tcaaagaggc ggaaaaatat atcggttatc cctatgtgtg gggcggcagc tctcccagca
    13921 ccagctttga ttgcagcggc ttcatttcgt gggtaatcaa tcatagtgga tggaacgtgg
    13981 gacggctggg ggcgcagggc ctatataaca tctgcacccc tgtttccagt tcgcaggcca
    14041 agcccggcga ccttgtattt ttcaagggaa cctacgacac ccccggcgtg tcccacgtcg
    14101 gcctgtatgt gggcaacaat gtgatgctgc attgcgggaa cccgatttcc tacacgaacc
    14161 ttacttcaaa ctactggcaa tcccattttt acagttacgg gcgtttacct taacgccaga
    14221 aaggagcttt caattatggc aaccaaactt gaccgcatcg aaaaggacat ccagaaaacc
    14281 aagtctaaga tcgcggagtt tcagaaacag cttcgggagc tggaaacgca gaaaaccgaa
    14341 caggagaatt tgcagatcat ccagcttgtg cgcggcatga acatgaagcc ggaggaattt
    14401 gccgcgttct tacgcagcgg cgcaatgcag gccgcccctg ccgcaacccc ttaccatgaa
    14461 caggaggaca atgcccatga agaataagac cagacggatt tcccgtattc tccccgcact
    14521 tctggccgtg atgctctgcg tcacggcctt tcccgttacc gcccttgcag gcggcaatga
    14581 cccggcaccg gccccgctgc cggaggctac aaccgaaccc gctacgggcg gtttggagcc
    14641 tgaaacagac gaaaccggca ccgccacgga cgggacagaa accgaggccg aaaaagatac
    14701 cggccctctg accggcaagg acatcaccga cctgttttcc gcgctgttcg gttccaaggt
    14761 gagcattgcc gccacggacg acggcatcca gatcaccacc ggcaccgaca aaaaagaacc
    14821 tgaacagacc ggcaccgtca ccaccaacgg cggcaggctg aatgtccgca ccggcgcggg
    14881 gcttgacaag accgcgttca cccagcttcc caatgggacg acggttgagg tagtcggcac
    14941 agacggcgac tggattaaaa ttctcttgcc ggagcgcatc ggctatgtcc attccgacta
    15001 tatgaccgtc agcgaaaagg aagtagcggc taccggcgag ggcggttttt ccctctccat
    15061 tgacccggag gaaattgctt ctctgctgga actgttcaat ggcggcgggg ccagcgccgc
    15121 cctgtccccg gacgggaacc tgtcactgat tgacgacatc ggcagcgccg cgaaaaatgg
    15181 caagcagttt atcaccgtgg aaaccaaaaa cggcaatgtg ttttatctga ttattgaccg
    15241 ggacgacgag ggaaaagaaa ctgtccattt cttaaatcag gtggatgaag ccgatctgct
    15301 gacgctgatg gaggacgggg aaaccgtcac ccccgctgct gtctgctcct gtaccacgaa
    15361 atgcaaggcc ggggctgtga acacgaattg cccggtatgt aaaaccaacc tgaccgagtg
    15421 cagcggcccg gagccgcagg aaccccagcc ggaggaaccc gaagccccgg aggaagaacc
    15481 caagggcggc gcgggcggcc tgatcgtgtt cctgctggtg gcgctggcgg gcggcggtgc
    15541 ggccctttac tatttcaagt tcaaaaagcc caaggctgaa accaccggca gcgacgattt
    15601 gggcgagtat gattttgggg aggacgagga tttggaggac gaggaacccg aaaccgagtt
    15661 tgacccggaa gctgattttg tgagtgaaca ggaggacgag gaatgacata taaacttgta
    15721 atcgcggaaa aacccagcgt ggcccgcagc attgcgggcg tgatcggggc cacggagaaa
    15781 cacgacggct atttggaggg gaacggctat ctggtgagct ggtgcatcgg acatctggtg
    15841 tcctttgccg acgccggacg ctatgacgag cgtttcaaaa aatggcgcta tgaggatttg
    15901 cccattctcc cggaaacatg gcagtacatc gtccccgacg acaagaaaca gcagtttgac
    15961 gttctctgtt cgctgatgca gcgccccgac gtgaccggcc ttgtgtgcgc caccgacgcc
    16021 gggcgcgagg gggaattgat tttccgtttc gtctaccaga tggcgggatg ccaaaagccc
    16081 tttgagcgcc tttggatttc ctctatggag gacagtgcta ttaaggacgg ctttgcccac
    16141 ctgaaaccgg gcggcgacta tgatccgctg tatcaatcgg ccctgtgccg ggccaaggcc
    16201 gactggctga tcggcattaa cgccacccgg cttttttccg tcctgtacca caagaacctg
    16261 actgtgggcc gcgtccagac gccgacctta aaaatgctgg tggacaggga cgccaagatt
    16321 accgatttca agaaagagaa atatcacatc gtccatatca cggcgggcgg cgcggacgcg
    16381 gtaagcagcc gcttttccga cgctgccgag gcaaacaccg tcaaggcggc ttgtgcaaaa
    16441 gcgcaggccg tttgcgtttc cgtgacgcgg gagaaaaaga cggaacagcc gcccaagctc
    16501 tatgacctta ccaccttgca gcgggaggct aaccggcttt tcggctatac cgccaagcag
    16561 acccttgact atgcccaggc actgtatcta aagaagctgc tgacctatcc gagaacggat
    16621 agtcaatatc ttaccgacga tatgctgccc gccgtggaaa gccttgtgtc cggcctgtgg
    16681 gagctggtgc cgtttgccaa gggcctgaac ctttccccgc agtttgaccg catcctgaac
    16741 agcaaaaagg tgtccgacca ccacgccatt atccccaccg ctgaatttgt gaagcagggc
    16801 tttggcactc tggccgagag tgaaaaaaag ctgctgtccc tgatctgctg caagctctta
    16861 tgtgccgtgg ccgggccgca tatctatgag gccgtcaccg ccacatttac ctgtgccggg
    16921 caggagttta ccgccaaggg caagaccgtc ctttcccccg gctggaaaga gattgaacgc
    16981 cggttccgtt cttccttaaa gaccgacgcc gacgaggacg ccgaggcggt gcgggagctg
    17041 ccggagcttt gcgagggcca gacctttgca gacgtggccg cctctgtcac cgagcatttc
    17101 accacgccgc ccaagcccta caccgaagat acgcttttgt ccgctatgga acgggccggg
    17161 gccgaggata tgccggagga tgccgagcgc aagggattag gcacaccggc cacccgcgcc
    17221 gccattctgg aaaagctggt gcagatgggc tttgtccaga gaaaaggcaa gcagctcatt
    17281 cccaccaagg acggcatcaa tctggctgtg gtgctgccgg aggcgcttac ttctccccag
    17341 cttactgccg aatgggaaaa ccggctgacc gagatcgcca aggggcaggc cgatcctgac
    17401 gaatttatgg acggcatcga gacgcaggcg cgggagctgg tgcagaccta ttcctgtatc
    17461 agtgaggaaa agcagaaatt gtttcaggcc gagcgtgttc ctatcgggac gtgtccccgc
    17521 tgtggtgagg ctgtctatga gggcaagaaa aactactact gcggaaaccg ggcctgccag
    17581 ttcgtcatgt ggaagaatga ccgatttttt gaggaacgca agaaagcgtt caccccgaag
    17641 atcgccgctg cgctgctcaa aaccggcaaa gtcaaggtaa agggcctcta ttccgtaaag
    17701 accggcaaaa cctacgacgg gacggtgctt ttggctgata ccgggggcaa gtacgtcaac
    17761 taccgcattg aacacaggag ctaaaggaca gtaacacagc aagcataggg agtgtggcta
    17821 cactcccggt aaaatccccc ggccgcgtcc ctgccgggcc ggggattttt gcttgttggg
    17881 gaagtcccca accccctatt ttttgaaaca gaacagagaa aggagtgacc gcgtgaacga
    17941 agtattttct atccaagtcc ataaccggca gtttttggaa cagggcaaac aaggcgtctg
    18001 gctggatttg cccaccacaa ccgaaacgct gcaagccgcc ctgcgggatg ttggcattac
    18061 tgccgacaat ccgcaggatt tttttatcaa cgggtattcc tgcccggagg acagacacct
    18121 tgccattccc tatgacatgg tgctggccgc cgacgtggac gagctgaatt tccttgccgc
    18181 ccggctgggc cagcttgacg ccgccgagat cgcagagctg aacgccgccc tgcaaaatcc
    18241 ccgtggcggc tttgagaata tcggccagat catcgactac accgaaaatg tggattacta
    18301 tgtgcatctt cccgacgtta ggggggatgg acagttgggc gactactacc tgaaccgttc
    18361 cggcatggtg gatatgccgg aggaatggaa agccgggatt gatacggcgc agtttggacg
    18421 ccacattgcc cagcaggaac aaggcgtttt tacccagtac ggctatctgg tgagaagcgg
    18481 cgacgaatgg cagcgcgtcc atgagggcca gccggtgccg gaggaatacc gggtgatgca
    18541 ctacccccag ccggaagctg accgggagga accgcaggcc cagcagccgg aggcatccga
    18601 aagccccgac aaagcgccgc ccccggttat cccgatcatg ctggacggca tgaacagcaa
    18661 agaacgcatg aaagtgatta ctgaccgtct ggaaaccggc gtacaggagc tttttgaaag
    18721 tagccgctat atggcttact tatccgctat gtccaagatg cacgattaca gtctgaacaa
    18781 tacgctgctg atcgccatgc agggcggttc gattgtggcg ggctataaca ggtggcagga
    18841 tgaatttgac cgccatgtga agcggggcga gaaaggcatt aagatactgg ccccggcccc
    18901 gttcaaggtt aagcagcaga tggagaaaac cgacccggcc acccagcagc ccgtgatcgg
    18961 cgcggacggc aagcccgtga ccgaagaaca ggaagtgacg gttccggcct ttaaggtggt
    19021 gacagtcttt gacatcagcc agaccgaggg caaggatttt cccatgccct atatcagtga
    19081 acttaccggc gacgtgcagc agtatcagga tttctttgcc gcgctggaac ggacatcacc
    19141 ctatcccata agcattgagc cgatttcaga cggggccaag ggccgctgct tctacgcaaa
    19201 acagcgcatt gccatcaacg agggcatgag cgaattgcag accctaaaga ccgccatcca
    19261 tgagattgcc catgccaagc tccacgccat tgaccgggag gcccccgccg aggaacaggc
    19321 ggcccggcca gaccgcagca cccgcgaggt acaggccgaa agcgtggcct ataccgtctg
    19381 ccagtattac gggctggaca cgtcggacta ttcttttggc tatgtggccg attggagcag
    19441 cggcagggag ttatccgagc tgaaagcctc ccttgaaacc atccgggcca cggccagcga
    19501 attgataacc gagatcgacg gccattttgc ccagcttcag caggagcgcg aggcccggca
    19561 ggaacaggag gccgtcacgg ttgacgttac cccggaggca gcggccccgg cgcaggccga
    19621 ggaagaacag gccccggccc ttgatccggc agccgagccg cttgtgactg tgctatggag
    19681 cgaaagcccc cagctaaagg acggccagca aatgccgttg catgaggccg acgcgctgtt
    19741 tgcccgtctg gatgcagcat atccggcaga ggcaggctat gacaaaaccg cgttccgcat
    19801 tgactgtatc tttcagggcg agccggacag ctacgagggg cggcaggatt ttggagatag
    19861 ggacggttcc ctgatcgaac atatccagag ctaccaccaa ttttacgcgc aggacgaaaa
    19921 ctggaaaaag catgtaatcg ctacaaccgg catagagggc tgggagcagg acaaggccca
    19981 tcgggatatg ctgttaggcg agtttgtccc ctatatgaag ctgcattgca gccttgccca
    20041 aatggaacag acggcgcagg gccgcttaca gtccggggaa accctgacgc cggaggaaac
    20101 ggcctatttg ggggcgcttc tctcttatgt gcaggagtgc cgccccatgc tcaatcaggg
    20161 ccagtatcag ttgccggagc cgccgaaact ggctgacttc gaccagagct tgcaggacta
    20221 caaagcacag gtacaggccg agatcgcgca ggaggccgcc gcagccggta tgaccgtgga
    20281 ggaatacgcg gcggctggct atgaagcacc ccaacaggac accttttcca tctaccagct
    20341 tcgaggcggg gaggaaaccc gcgactatcg gtttgagcca tacgagcgtt tacaggccgc
    20401 agggctggcc gtggacagtg ccaactatga ccttgtttat acggcaccgc tggataaaag
    20461 cacgtctttg gaggatattt accgcacatt caacatcgac cacccggcag attttagggg
    20521 acacagcctt tccgtttccg acgtggtggt gctgcaccag aatggacagg acaccgccca
    20581 ctatgtagac agcttcggct atatggaagt gccggagttt ttgcgggaaa atcccctgcg
    20641 taccgccgaa ctgtctacgg agcagaacga aaacatgatt gacggcatta tcaacaatac
    20701 gccgcccgaa ccgcagccgg aggaacccga acagctatct tttgaggatg tggcagttgc
    20761 ttccggccag acacaggagg aacccccgga ggctgcgccc ccgcaaacgg ctgtccgcta
    20821 ttatcccatc aatgagggag ccgcccgccg cgccaaggaa gccatgagct tttctgacta
    20881 ccggcccggc agcgccacgg cggaataccg gcactatgtc gatcaggccg tggaaattgc
    20941 ggagcgccag aaaaagcgtg tggagccaga gtatcacgaa aagatagaca gcctccttga
    21001 cacctacgcc cgcaagctgg ccgagaacat gaataagggc tatgagatta ccgcccgcgt
    21061 cccctctgtc ctgattgccg ggccgtccaa tttcccggta cgggcgaaag aaaagcagaa
    21121 cgccgccagt gaccgcaaca tggaggaatt tcagtacatt cagggcttgc ttgacaaaat
    21181 ccgcagcacc ggcatgggtg gtatcagcgc cgacgatccg caggccgttt ccaagctgga
    21241 aaagaaactg gaaaagctgg aagcgtcgca ggagttgatg aaggccgtca atgcctacta
    21301 ccgcaagcat gggacgctgg acggatgccc ccatctgatc gaaagaggca ttgaaaacct
    21361 gaaagcggat atggcgtcgg gctggcacta tgagaaaaaa ccgttccagt cctggcagct
    21421 ctccaacaat aacgccgaga tacgccggtt aaagggccgc attgaggaat tgacccgcca
    21481 aaaggaggcg gcctatgtgg gatgggagtt tgacggcggc actgtggaga tcaaccgggt
    21541 ggccaaccgc ctgcaaatct tctttgaggg gaagccggat gctgctgtac gcgacgagct
    21601 gaaaagcaac ggtttccgct ggtcgcccaa ggccggggca tggcagcggc agttaaacga
    21661 caccacaatc cgcgtcgctg accgtatcaa gtgcatccag cccctttccg gcgaaaagcc
    21721 cagcgcctta cagcttgcgg cccgcaggga gaaagaaaag ccctctatcc gggcgcagct
    21781 caacgcggcc aagaccgaac aggccaagca gccaccggcg cgggaaaaat caaagggatt
    21841 ggaggtagag taaatgcaga agtttgaaaa tgtggacgtt ctggccgcgc tggaacagat
    21901 catgcggcag aacaccgcgt tttatcaaag tgactttggc attgacaaag acattatcag
    21961 gcgggatgcc gccagcgatc aggccgcaga caagaccctg ctctggatgt cccggccctc
    22021 cgggacgtac tgtttccggg aacgtgatgt tttcctgaaa gacacccgac agtacaacac
    22081 atggaaattt tacggggagc agacccgtga caagatactg gcctacgcgg tggagctgac
    22141 cggcacacag ggcggcacta tccggggcaa tctctatgag ctggactatc cgcagcattt
    22201 ccggcacgtc attgaaaccg cccagccggt gagtgtgaac cggctctttt atgagcatgg
    22261 cacccgtgac atcccggaga gccagcgttt tgacggaagc cctgaccggg tattgggaaa
    22321 ctttttgtac tatgaggccc agccccatga cccggctgtg ctgcaggagg cgctacggac
    22381 ggagcagcac agccgggggc gggccgtccc cggcgatttc aaagtccacg tcaccgcttt
    22441 gcatgacagc cggattgaaa ccgaggcccg gcgtattgtg gacagcctga aagatctgtc
    22501 cgcgcctaac agccccaata aaacgcattt catggtggag atctccccgc actttgcggc
    22561 gattgcttcc accaaggata atgaccggct ctttgccatg ctgccctata aatccctgtg
    22621 ttttaccggg atgaaaggca ggcatggcgt gtatgccgtg attaaccggg acgagcgccg
    22681 ggatgtgagt attcgcaggc cccggccctc tatccgcaag cagctatccg acagcaaaca
    22741 ggccgccagc ccaaaaaagg cggcggccag aaccaaaaag aatgaattgg aggtataagg
    22801 aatgatgcac tttaccgtgg aggaagaaaa cctgatctgt atgtaccaca atgcagaccg
    22861 gcgcagaacg ataaacaggc tcaccgccgt catgccggtt atggatgggg atatgcgggc
    22921 gcaggcccag cagacccttt ccaagctgga acgcatgacg gatgccgact atgacggcca
    22981 gaagttccac ttcacagacg aataacgaac agcagacgcc gggcgcggcg gccatgtggt
    23041 cgcttgcgtc cggcgtcttt tctttttgcc tattcctacg catttagaac ccggtattgt
    23101 cggggatagg atgaagtatt cgcttgcccc tgcaaacatg cgggagaagc cttgcagcgg
    23161 gcggttttca atcccttaaa tgcgtaggtt gcagctataa ttagacccct gaaacagata
    23221 tagccgcgca gcgggtgtat ctgtttcagg ggtgtgcagg tgatagccgc aaagcggcgt
    23281 aagagggtgc agccctcttg ccgtctatct ctgttccagc gggaagctcg gcaatgcttc
    23341 cagcagtttc agggaaatgc gctgccgtat atcttcgtca atgactttct tctttttgcc
    23401 gtttgcctgt ctgatctcca ccgtggaaag ctcgttgatg tagtcagcat aatgggccaa
    23461 cactttttcc gtggcccacc tctcaccggc aacggcggct ttgatcgttt catagtccag
    23521 cagcttttct tgtccgtcca tcctgatacc cccgtcagac tgattttttc tatgatacca
    23581 tattcggaag catggaacca gagaaaacgg cgcttttctg ctggtaaatt ctacgcattt
    23641 agaacagcac ttcggcgggg ataggataaa acacccacat accccgctga ccatgccgga
    23701 aaagccttga aatataaggg cttttacagc tctaagtgcg taggtttcca gtatcttttc
    23761 cgctggcgct cggcctcttt gcggcggcgc atccgtttgg agcaggccgc gcagtatttg
    23821 gcccggttag acttcggcgt gaacgtggcc ccgcactcac agcagcgttt cacttcgtcc
    23881 cggtctttgg tgatctcggc atacagggcc gcatccagcg ggaggacagc ggccttaaac
    23941 cagcggcaca gaagcgaata ggaaatgctc tgtacgcaaa tacattcttc gccgtcgtcc
    24001 agcaacaggc agttgccgtc acaatagttg cagcactcat gcgtcagctt ccgggccttg
    24061 cgatattgag ggtaattcat tcgtggaata ctcatagctc ttgcgtccat tcctttcccg
    24121 gcgtcatacg caaaatgccg tccacattct gcttgatgat gccgtattcc tgcatctgct
    24181 ttttcgcttc gccgtacttc tcatacagcc gttccttttc ctcggccaat cgcttgtact
    24241 cggctttcag ggcgtacaga tcggggagct ttttcacacc ctgtcccttt agggcctttg
    24301 ccgcagcttc atacaaaatc aacgtgcttt catgctcacg ccggaatttt gccttatccg
    24361 ctgcattgcg gtattccata gccacgggcc agagctgctt gtaggtagaa atattcttta
    24421 tcagcaccgc catatccgca agccggtgtt ccatagcttt gagggtagcg gcggcctcgt
    24481 cgctggcggc ggtgatctcg gccactttgc tttccaaatc cgggtaggtg tctatcccat
    24541 gttcagtcag gaaattcaaa gtctttgcgg cctgcttcaa attgtggagc ttcgcccacc
    24601 tctcatagcc tgccgactgc tgggccttaa tgttgttttc cagatcaatc cgcaaagaaa
    24661 tccccttgcg ctctttgggc gctctggtga taacgcggcc tttgatacgc tctttgatgg
    24721 cttcctcggt gtaggcttcc cccaatgtct tgcagcgggt aaagcgttcc tgtccgggag
    24781 cgcggaaaga aataaacttt cccggcttga tctcatagcc ctgcgcctgc atgagccgca
    24841 ggaaatcatc aaaatctttt gcctgcggga tagcggcgtc aatggctgct ttgagcttcg
    24901 ctttccagct cgtccctttc cgctgggcgt cccactcggc atagctcttg cccttactct
    24961 ggccgggctt caccacggac aatccgtttt ccttgcacag cctgtcgctg gtgcgccgga
    25021 tataggcata gctctgtttg ttggaaatgt acttgcgctg atttaccata tccacggcac
    25081 agaaaataat gtgattgtgg acgtggccct tatcaatatg ggtagtcagc acatagggat
    25141 atttcccttg cagcacttcc tcggcaagct gcttcccgat ctcatgggcc tgttccggcg
    25201 tggcttcgcc gggggcaaag gattgtatca ggtggtggcc cagattattg cccttttggt
    25261 atgcctgcgc cagcagcatt tcaaattcaa tgtccgctgt ttcataagag caggcaaagg
    25321 aggccaccag cagcttgccg tcagttttgt ccgggttctc gatgtagtca agggcttttt
    25381 tcagcgttga cttgatagga tgtatctttg taactgccat atctgcgcca gcgccccctt
    25441 tatatcctcg atgtcctgtg cgtacaccgg ccccataccg ttcagcctct ttgcaatctg
    25501 attgacgttg acgccgatct tctgtatctc ggcggtctgc gcccggatgt cgctggtgtc
    25561 catgttgacg atatagccgt caatgagcat cttccgggcg taggctgaaa agttcttcgt
    25621 atgcagcagc gccattttct gttcaatcat agcccgttcc tccggcgtca cagggacgcg
    25681 cagcacgatt tttcgctttc tgtttgccat gcttcaccgt cctttccatt caggggtttg
    25741 gggatttccc caacaagcaa aacggacgcg gcccggcagg gacggggcag gccgtttttc
    25801 cgggagtggc tacactccct atgcttgcta cgttactgtt tctccactaa caagacgttt
    25861 ccaaagcccc aaactacccg caaaatagcc ctctggaaag aaaaaagtgc aaaaaaatag
    25921 aagctacgct gtgcagctcc cgctaaaagt aaaacagagg atgccagtta cacatcctcc
    25981 gtttcattct tcttcggctt ttcaattagt atggcgctgt caatcgcgcc ctcgatgatc
    26041 ggcaggtatt cttcggggca gagtttcagc ttgtggctga ccctttgccg cagttcgctt
    26101 tcctcccgca tgatctccgg gttaaagtag cgttccacag gaagcccgca ggctttaata
    26161 agctgtatga caaccggcag actgggaatg gcccgtcgtt ttcgatgttg gcaagatagc
    26221 gccagtcaat atttattttc tctgccattg ctttgcgcga aacgcgcttt gcttgccggg
    26281 ccgctttcac atccgcgccg aaagtttcaa agccgggaca atcttcaata ttcgccatat
    26341 cgcacctccc acttacattg tatatttcat actctgcccg tggaatgttg gtatatgcac
    26401 ctttcttaaa gtttatattt cacattttgt ttcttttatt cttcgagcaa aagaattata
    26461 atgtaccctg tggaggtgta ctatggacta catgacgttg aaagaggcag gcgagaaatg
    26521 gggtgtgccc ccacgatgga taaactacta ttgtgctgcg ggacgcatcc ccggcgctgt
    26581 aaagatggcg acgatctggc tcattcccaa aactgcggaa aagccgattg acaggcgtac
    26641 aaagaaagga aaggcggtta ctgaatagta taatgaaacc ggcgcatttt ggatatggtt
    26701 ttgaagagag agaaagcatg gtattttgtc acggaaaaac tataagatga tagatggcag
    26761 gctgctccag accgataaaa agtattctca tctgaaacag aaagaaaaga tttacacatg
    26821 gatgtatgaa gaagcgaaac tttatcatga cagaaatgga aaatgcccga aacaaagacg
    26881 aggaagctgc tgctgtatca gccgtatatg gtagaattga aaaggccggc atctggattc
    26941 tttatggtga agtctccaga cattggataa ctgctggtct aatgcgcctt tgagtatggg
    27001 gtgattgatt ttaataccgg ggaggtacgg ctttttgagg aaaatattct gggatttaca
    27061 tttggcgatt attacatttc ctgtaactgc aaggacacgg atgatgaaga tatgaggaat
    27121 atagacttgc tgattttcta ctgtcttgaa cggtattagg actgaatctg ctgcgcggag
    27181 tgcggcaaga gcgaccggag ggagtacaca caggccctgc ctgtgtgaaa agccataccg
    27241 caaggtgcat tcgcaaaaaa gaggccgcgc attttgctga ggcttggggt gtccccaaca
    27301 agcaaaacga gaacgccgca ggcgggcaag tggtggatca gaatatccgt aaaggatatt
    27361 ctgatccatt gcttgccagt attatcggga tgttttgaca accgctgtaa tcgaaacatc
    27421 ccaaacatta agaaggtgtt aattattatt atcttgattt aattcgttta aaccaataat
    27481 atagatacat acaccatgaa aagaagcgag tatcccaatg acggatgatt aaaatccgaa
    27541 aagaaatcgc tttgaaacta cagggaaact acagactgtt atgttatctt cttaaaatgg
    27601 agggattttt atgtcgatac gaattctact tgtcgaggat gatgatcata tctgtaatac
    27661 agtaagggcg tttctggctg aggcaggata tcaggtggat gcctgcacag atggaaatga
    27721 ggcatacacc aagttttacg aaaacactta tcaactggtt attcttgata ttatgctgcc
    27781 cggtatgaac gggcatgaac ttttgcgtga atttcgtgcg aaaaatgata ctcccattct
    27841 gatgatgaca gccctgtcgg atgacgaaaa ccaaatccgg gcgtttgatg cagaggcaga
    27901 cgactatgta acaaagccat tcaagatgca gattttacta aagcgggtgg aagccctgct
    27961 gcggcgcagc ggtgcgctgg caaaggaaat ccgtgtcggc aggctgacac ttctgccgga
    28021 ggatttcacg gtactttgtg acggtacaga gctgcccctg acacgaaaag aatttgaaat
    28081 ccttttgctg ctggtgcaga acaaaggcag aaccttaacg catgaaatca ttttgtcacg
    28141 tatatgggga tatgactttg agggtgatgg cagcacagtc cacactcata tcaaaaattt
    28201 gcgggccaag ctgccggaaa atatcatcaa aaccattcgc ggtgtaggat accgattgga
    28261 ggaatcataa taatggaaag aaaagggatt ttcattaagg ttttttccta tacgatcatt
    28321 gtcctgctac tgcttgtcgg tgtaacagca acactgtttg cacagcaatt tgtgtcttat
    28381 ttcagagtga tggaattaca gcaaacagta aaatcctatc agccattggt ggaactgatt
    28441 cagaatagcg ataggcttga tattcaagag gtggcagggc tgtttcacta caataaccaa
    28501 tcctttgagt tttatattga agataaagag ggaagcgtac tctatgccac accaaatgcc
    28561 aatacatcaa atagttttag acccaacttt ctttatgtgg tacatagaga tgataatatt
    28621 tcgattgttg ctcaaagcaa ggcaggtgtg ggattgcttt atcaagggct gacaattcgg
    28681 ggaattgtta tgattgcgat aatggttgta ttcagccttt tatgcgcgta tatctttgcg
    28741 cggcaaatga caacgccgat caaagcctta gcggacagtg cgaataaaat ggcaaacctg
    28801 aaagatgtac cgccgccgct ggagcgaaag gatgagcttg gcgcactggc tcatgatatg
    28861 cattccatgt atgtcaggct gaaagaaacc atcgcaaggc tggaggatga aatcgcaagg
    28921 gaacatgagt tggaggaaac acagcgatat ttctttgcgg cagcttctca tgagctaaaa
    28981 acgcccatcg cggctacaag cgttctgttg gagggaatgc ttgaaaatat cggtgactac
    29041 aaagatcatt ctaagtatct gcgcgaatgc atcaaaatga tggataggca gggcaaaatc
    29101 atttccgaaa tactggagct tgtcagcctg aatgatggga gaatcgtacc catagctgaa
    29161 ccgttggaca tagggcgcac ggttgccgag ttgctgcccg attttcaaac cttggcagag
    29221 gcaaacaacc agcggttcgt cacagatatt ccagccgggc aaattgtcct gtccgatccg
    29281 aggctgctcc aaaaggcact atccaatgtc atattgaatg cagttcagaa cacgccgcag
    29341 ggaggcgagg tacggatatg gagtgagcct ggtgctgaaa aatgccgcct ttttgttttg
    29401 aacatgggcg ttcacattga tgatactgcg cttccaaggc tgttcacccc attctatcgc
    29461 attgatcagg cgcgaagcag aaaaagtggg cgaagcagaa aaagtgggcg aagcggttta
    29521 ggacttgcca tcgtacaaaa aacgctggat gccatgagcc tccagtatgc gctggaaaac
    29581 acctcggatg gcgttttgtt ctggctggat ttaccgctca catcaacatt gtaaatattt
    29641 aaaacttaaa tgatttt
//
LOCUS       DADVXU010000023        29097 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.025, whole genome shotgun sequence.
ACCESSION   DADVXU010000023 DADVXU010000000
VERSION     DADVXU010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 29097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.025"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..1031)
                     /locus_tag="KB953_001003"
     CDS             complement(<1..1031)
                     /locus_tag="KB953_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB6865993.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1113..1802)
                     /locus_tag="KB953_001004"
     CDS             complement(1113..1802)
                     /locus_tag="KB953_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBB6865994.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="KB953_001005"
     CDS             complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="KB953_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBB6865995.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2329..2832)
                     /locus_tag="KB953_001006"
     CDS             complement(2329..2832)
                     /locus_tag="KB953_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBB6865996.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3036..3163)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="KB953_001007"
     CDS             complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="KB953_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBB6865997.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="KB953_001008"
     CDS             complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="KB953_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBB6865998.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4800..5468
                     /gene="sdaAB"
                     /locus_tag="KB953_001009"
     CDS             4800..5468
                     /gene="sdaAB"
                     /locus_tag="KB953_001009"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HBB6865999.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5481..6353
                     /gene="sdaAA"
                     /locus_tag="KB953_001010"
     CDS             5481..6353
                     /gene="sdaAA"
                     /locus_tag="KB953_001010"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HBB6866000.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6444..7463)
                     /locus_tag="KB953_001011"
     CDS             complement(6444..7463)
                     /locus_tag="KB953_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBB6866001.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7636..8541)
                     /locus_tag="KB953_001012"
     CDS             complement(7636..8541)
                     /locus_tag="KB953_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBB6866002.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="KB953_001013"
     CDS             complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="KB953_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HBB6866003.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9246..9416)
                     /gene="secE"
                     /locus_tag="KB953_001014"
     CDS             complement(9246..9416)
                     /gene="secE"
                     /locus_tag="KB953_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBB6866004.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="KB953_001015"
     CDS             complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="KB953_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBB6866005.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9754..10398
                     /locus_tag="KB953_001016"
     CDS             9754..10398
                     /locus_tag="KB953_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBB6866006.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10454..10957)
                     /locus_tag="KB953_001017"
     CDS             complement(10454..10957)
                     /locus_tag="KB953_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBB6866007.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10970..11016)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11554..12519
                     /locus_tag="KB953_001018"
     CDS             11554..12519
                     /locus_tag="KB953_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HBB6866008.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12618..13790)
                     /locus_tag="KB953_001019"
     CDS             complement(12618..13790)
                     /locus_tag="KB953_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6866009.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13777..14922)
                     /locus_tag="KB953_001020"
     CDS             complement(13777..14922)
                     /locus_tag="KB953_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6866010.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14936..15868)
                     /locus_tag="KB953_001021"
     CDS             complement(14936..15868)
                     /locus_tag="KB953_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6866011.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15985..17064
                     /locus_tag="KB953_001022"
     CDS             15985..17064
                     /locus_tag="KB953_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBB6866012.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17061..17679
                     /locus_tag="KB953_001023"
                     /pseudo
     CDS             17061..17679
                     /locus_tag="KB953_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(17789..18016)
                     /locus_tag="KB953_001024"
     CDS             complement(17789..18016)
                     /locus_tag="KB953_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBB6866013.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18119..20707)
                     /locus_tag="KB953_001025"
     CDS             complement(18119..20707)
                     /locus_tag="KB953_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HBB6866014.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20727..21386)
                     /locus_tag="KB953_001026"
     CDS             complement(20727..21386)
                     /locus_tag="KB953_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBB6866015.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21435..21938)
                     /gene="trmL"
                     /locus_tag="KB953_001027"
     CDS             complement(21435..21938)
                     /gene="trmL"
                     /locus_tag="KB953_001027"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBB6866016.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22159..23025)
                     /locus_tag="KB953_001028"
     CDS             complement(22159..23025)
                     /locus_tag="KB953_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBB6866017.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23134..23775
                     /locus_tag="KB953_001029"
     CDS             23134..23775
                     /locus_tag="KB953_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBB6866018.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23837..25198)
                     /gene="mgtE"
                     /locus_tag="KB953_001030"
     CDS             complement(23837..25198)
                     /gene="mgtE"
                     /locus_tag="KB953_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HBB6866019.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25216..26115)
                     /locus_tag="KB953_001031"
     CDS             complement(25216..26115)
                     /locus_tag="KB953_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBB6866020.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26117..26914)
                     /locus_tag="KB953_001032"
     CDS             complement(26117..26914)
                     /locus_tag="KB953_001032"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HBB6866021.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26892..27596)
                     /locus_tag="KB953_001033"
     CDS             complement(26892..27596)
                     /locus_tag="KB953_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HBB6866022.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27727..28302
                     /locus_tag="KB953_001034"
     CDS             27727..28302
                     /locus_tag="KB953_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HBB6866023.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28420..29097
                     /locus_tag="KB953_001035"
     CDS             28420..29097
                     /locus_tag="KB953_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HBB6866024.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8560 a         6167 c         5232 g         9138 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactgc acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
     1201 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
     1261 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
     1321 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
     1381 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
     1441 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
     1501 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
     1561 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
     1621 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
     1681 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
     1741 actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat tttctttttc
     1801 atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa
     1861 agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt
     1921 acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg
     1981 ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa
     2041 ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat
     2101 tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct
     2161 acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt
     2221 tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata
     2281 atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc
     2341 tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg
     2401 tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt
     2461 gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt
     2521 tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa
     2581 tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc
     2641 tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg
     2701 tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga
     2761 tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat
     2821 tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga
     2881 aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca cctattcact
     2941 tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc
     3001 agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa actctatgcc
     3061 accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta ggaaattaag
     3121 gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact agcttagcat
     3181 gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt ctaatattta
     3241 attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg atcgttacat
     3301 ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca tcatggattg
     3361 ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt ggtacatgga
     3421 tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg tttactgctt
     3481 ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct tttggtccta
     3541 atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca actacatcaa
     3601 agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg aaatctgcac
     3661 cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt
     3721 taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct tttttagggt
     3781 ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt
     3841 ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact ttttttaatg
     3901 catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg tggttataac
     3961 ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga agctgctgtc
     4021 ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg
     4081 cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc agcgatttct
     4141 cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc tgaacctttt
     4201 tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt aacgaatgtg
     4261 aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc ttgatcagct
     4321 gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg acctagtgca
     4381 ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt aacgattttt
     4441 tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta attggagatg
     4501 catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa
     4561 caatgcacac tggtacagta tagcaaattt atttgttttt tcaagatttt tttctgctat
     4621 ttttacattt tgcttgaata aacgctaaat ttattctaaa aaaacaacaa tttttcgttc
     4681 tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa agcaaacaat
     4741 aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag gtggagaaaa
     4801 tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg attggtccaa
     4861 gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt atatttggcg
     4921 aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc tatcgggggc
     4981 acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat gatgaacgtt
     5041 tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt gttcctaaga
     5101 gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga acacgaaagc
     5161 tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa ttgaatggat
     5221 tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa gatgttccag
     5281 gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc ggcactatga
     5341 cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta gatgatcctc
     5401 aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc gtaaattatt
     5461 tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat caagcaaaag
     5521 cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt ggccgttccc
     5581 gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa tcaatcaacg
     5641 agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca aaaaaattag
     5701 atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa gctgtacgca
     5761 atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct acaccgactg
     5821 ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg cttgatttga
     5881 cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa
     5941 acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt tctgcaagtg
     6001 caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag
     6061 cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt gcaggcttag
     6121 ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt atctctgctg
     6181 atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt
     6241 accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg
     6301 ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg tgataaagct
     6361 ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa gagaggacta
     6421 attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac ttcatcgatc
     6481 gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca
     6541 gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg gccaatcgca
     6601 actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg gacaccagag
     6661 tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa tacttcaaag
     6721 gacgcaggac ctccatctag ttgacgtcca ccatgattgg atacccagat accatctgcc
     6781 cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac gataactgga
     6841 agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg ttttgcagct
     6901 gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg ttcgctatac
     6961 gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc ttcttcacgg
     7021 taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc ttctactgct
     7081 ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg gaaaaactgc
     7141 ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata agtgctgatg
     7201 gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc tttttcatgt
     7261 gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc aattccgaaa
     7321 acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat gattttcttt
     7381 gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc accgcgaata
     7441 tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta ggcaatgttt
     7501 acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga tccttctttc
     7561 atcttcttga ttacatataa atcatactaa acttacaaaa agtaagcaaa catttactct
     7621 cagatacttt tatcgctatt ctttccaaat aaatcgactt acaagctgat ctacttcttg
     7681 gttttcatga agcatactat gagaggcatt ttcacctgtc actacttttt cctctatttc
     7741 atttccgcgt tgcttcaaca acgcatagac cgctaatgca ctgtttaacg gtactgttcc
     7801 atcactcaag tctgtttcat ctagttgacc agcaatcaag aaaaatcgag tggttatagg
     7861 aatattaccg atcatttgct ggtaatcttg atagcggtta gaaacgacag caggcccctt
     7921 tgctaactca tctgtcagag actgcgcact atcatcgaca aaatcattaa aaggcgcgcc
     7981 aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata
     8041 gcgtagtgca ctaactcctc ccatggaatg ccccactatg ttgactttat ctatgccgta
     8101 gttttctttc agatacaaaa gacaagtttt gatccattct gcttgattcc actcgttatt
     8161 tttattatct tcaaacaata cttggaccat cggattgtct gcttttccag acaaatctcc
     8221 tgttgcttgt atttgtccat ctgttcctac tgttaaaacc aattctttta ctgcttgctg
     8281 atttttttcc aaacgccgca acatcccttt aaaggaattt tctgtcccgc tgtagccgtg
     8341 gaaaaaaagt gttggaatag tggttttttc tatcacttta ctggattctg ccgttttttc
     8401 tttatcagaa acttcagtag tatttatctt cgtcgtctgg caaccactta acgcaaacaa
     8461 actgataagg actaaagctg ctgcagttgc acgtttgcat aaatttttga ttggttctgt
     8521 catacacctc ttcctttcca tatagaaaaa agaaagactt gaaaggccaa tcatatgatc
     8581 gacatttcaa gtctctttat taatcgattt tatctacttg atcgaaatca agttctgtac
     8641 tggtttcacg tccaaacata tcgatgttga ctttcaattt ttctttttct ccgtcaactt
     8701 ctgttacttg tccttctaat ccagcaaatg cgccttcgat gattttcacg acttcgccaa
     8761 cttctacttc tagatcattt tgtcgcaggc tcataccgat agaacgcaag atatgattga
     8821 cttcttctgg aagtaatggt gctggtttgc ttccggctcc gtgtgaacca acgaatcctg
     8881 tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc atttcaacaa
     8941 gtacgtatcc tgggaatgtt ttatgaacga tttctttttc tttcccattt ttgacttcag
     9001 tttctttttc ttcaggaaca acgacacgga aaataaagtc ggccattttc atactttgcg
     9061 cacgagattc gatatttgct tttactttgt tttcatatcc tgaatatgta tgcaagacat
     9121 accagttttt ttcaaaagat tccattgaag gctcctcatt tcattgtaaa ataaaaaaac
     9181 ctcagtacac ccgaagtttt tctttcgctt cattatagca ctaaaagtac gttgtggcta
     9241 gcgagttatt ttaaaatcca gccaaataag ctttggatgg ctgtatccat tacgtaaaac
     9301 atcaatccga aaataataga agtttcgatg actactaacg tatctttacg gagttgtttt
     9361 ctgcttggcc aagttacttg tttgatttca tcttttacgc tttttagaaa tttcatgaga
     9421 caacctccct tgctactgtt tatttcgttt ctttatgtaa tgtatattga ttacaatatt
     9481 tacaaaactt attgatttcc aaacgttcgc cgctttttcc ttcactgact gattttgtgt
     9541 agttgcgaga cccacaaacg gaacaagcga gtgctgcttt tttctttgcc atttacttct
     9601 gcccccttgc tgagaatcag acttatactt cataacagta tcacgtaact ttttacttgt
     9661 caatttgtga aataaactat ctattgtttt ggataaaatt atgatatgat taatttgaaa
     9721 ttaagctaaa atgagacaat tggaggtata gcgatgctat taaaaacact tgtatataag
     9781 aaaaaagatc tgacaaccgt tacagagact agtacattag aagaagcatt aaaaatttta
     9841 gaaacttccg gataccgttg tgtgccgatc ttagattctt caggtaatat tttccgtgga
     9901 aacatttaca aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct
     9961 gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc tggatacttc tttcttcaaa
    10021 gtattcttta cgatcaaaga gcttccttat attgctgtcc ttgacagtga aaatcatttt
    10081 tatggtattt tgacacatag tacgttgctt aatatgctct ctcaatcttg gagcgtagaa
    10141 caaggaagct atgtactgac aattgcttcc acaggaaagc aaggagattt ggctgctatt
    10201 gcaaaaatca tttcaaaaca cagcagcatt gccagctgta ttactcttga tatcgaaaaa
    10261 gacgaataca ttcgacgtac attgatcacg ctaccggcta acactgaagc gaaagtatgt
    10321 gaaacgatcg tcaaccactt agagaaaaag aattataaag tggtagaaat tgaaaatttg
    10381 caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac agctaatctg ctttcttttt
    10441 tcgcttttta taattagaca cgcttttcaa aatcaagcgc gcgatctaaa taatacatca
    10501 atggtgctga aacagccatg acgataaact cacttaatgc aagcgtgcca taggttgtcc
    10561 aaaatgcttg gccacctgtt ggaactaaca tataagcaat cagtcccatg ctgatggtaa
    10621 agaagatcgt gttcaatact aaacgcatct ttacattttt cactttgttt tgcaataaag
    10681 ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa aaccacatcc atgataccaa
    10741 aaccaaagaa ggcattatag acgatgaccc cgcctaaaac tccccaaagc aatttacggt
    10801 taaagacaac taaatgattc aaactttctg aaatacgaaa ctggatcggc cctgaggata
    10861 caggcgcaac taaaatagtt aacgctagat aaagagccat gatgatgcca tttacaacga
    10921 ttacggataa ccgttgtctg cttttcgttt tttgcatttt ctctcctctt ttctcctagt
    10981 ttttttaacg cgggatggtt gatgaaccgc ggttatgtga aacaaacaat aattataaca
    11041 gacttttcat ttatttcaat ccgtcttatc tgcttttaaa aaagcaaaag cttaaacaat
    11101 gtaaacgttt gctaaaaatc gtgggatatg gtataaagta agtgtaaggg gtcataagaa
    11161 ttcccagtaa agcagacgaa gtaggatctg caagaacaga attgttgtga aacttaaata
    11221 aataaaaaag gatgtgggaa tttgagcaac ctcttactag agaacggtct agcaagcagc
    11281 tgaagatcta agtggacatg ccgaaccttg gaacttctcc ttacgtaagt aatggatcat
    11341 gcgcttgcta ggcttttttg tgtttttcgg tcttcggacc tttttcattt tcctagactt
    11401 ctgctataat aaccaagaat taattttgta acagattttt ttgaaaaaac aagtttcttt
    11461 cattcaaaaa atagaaaaag cttgctaatt attggttaac cagcaaacag gagtattaaa
    11521 atatcttgtt ttattgaata gcaggaggaa atcatgaaat tccattatca tatattgatc
    11581 aatccatcgg caggcagcgg aaatggctat aaagtagcag aaagaatttt accggtatta
    11641 aaaaataagc atattgacta tacacttcat tatagtgaat acaaaggaca cgatgctgag
    11701 attgctgaaa ctttggcaaa agaaacattg attccttgga tagaagaaga acagaaaaca
    11761 atcgatactt tccccctttt gatcatcgta ggaggtgacg gcaccttaca tcaggtactt
    11821 gatactttct atcagatgga agtagaattc ccagttgctt atattccagc aggttctgga
    11881 aatgattttg ctcgaggggc aggccttcca aaaaatccca aaaaaggact tcagctgatt
    11941 ttagcagctc aatcaccaga aaaagtccac ataatggctt atgaagaaaa aataagtgaa
    12001 aaaaaaggaa tagctgttaa taatttcggt ataggattag atgcagctat cgtccatgcg
    12061 acaaatcatt cttccacaaa aaagcgattg aacaaataca acttaggatc attttcgtat
    12121 ttattctcta ttctccgtgc tttattcact caaaaaggat ttccaattct ggtagatgca
    12181 ggaggaaaac aattaagttt ttcaaacgca tttttatgta cagcaacgaa gcatcctttt
    12241 tttggcggag gaatcgcgat tgttccaact gctgatgcgt ctaaaccagt cattgatttt
    12301 gtcgtggtcg aacgaatcaa tttctttaaa atcttgtggc tcattctttt acttatacag
    12361 aaaaaacaat tgaaatcgaa atatttccat cactacacaa caaacagatt acgtatcgtt
    12421 tcaacaacac cgcaacatgg tcaagaagat ggagaagaaa tggaaaaacg accttttgat
    12481 atcacaataa ccacaactaa acagttgttt tggtgttaat aaaagaggtt ttgtcagaag
    12541 ctacatctgg aacaccgttt attcggaaaa aaggactgtg acacgacttt tgtcacagtc
    12601 ctttttttac tatgcagtta attctttgcc caattgggtt gccatcgata gtttctttct
    12661 ttacccaaaa taaattggat cattgaaaac accgcagcaa aagcaactaa gacacaacca
    12721 ttgaaccaaa aagattctgc aaatgtttga ttgtaactga gtgtttcact gatcaagtta
    12781 ttattttgaa caaaccaata tgttggtgtg aatgccgcta ttttgctaac catttctggc
    12841 aagtattctg ctggtacaaa caccccaccg ataaagcaac tgcctgtgat aaaaatatct
    12901 ttgatcccac cgctggtttc tgagtttttt accaatgaag tgatacaagc cgaaaagcta
    12961 atgatcggca ataaaaacaa gaaagtattt aaaatatgat accataccgt tatatcccac
    13021 gaggtgtgga tcactaacag aaccattaga agtaagaagc cactgaacaa cagcagcgag
    13081 taactaatca aactaaaggt tactttgcga ttcaagtttc gtttggtaat gggcgcactc
    13141 tggtttcgtt tactgatctc tttgcgatta aacgctagat tgatgacgct gaaaccgcta
    13201 aaaatggata gaaacaaccc ataagacaag aaattaaagg ttgtgcccgt caacagcttt
    13261 ttcatccgct gactatatcc cgcacttagc tcaacttctc cagtaaccga gagtgccatt
    13321 tgtgtctgtt ccatagctgc atcgattgtt ttatcttctt cttgaaacag aatcagtgtt
    13381 tgcaaaaagg tgttcacttg ctgtgtaaca actgttttag aaaaggcatc tgggcgactc
    13441 tgtatcttga tcgtcgccat ctttcctact tcgacttgtt cggtaaagtc tgcaggcaga
    13501 tgtaagataa tgtttacacg attaaaataa agagcatcat cgatttcttt ttgtgaggtt
    13561 ccatccaatg taacgactgt ttccttgtca ttcaaatatt tacgaaggcc atcgactaca
    13621 ggcgactctt cttcactaat caaggcgatt ttcgcatctt gcagagaggt attttcaaca
    13681 tcaatatttt gcgcaaaaaa gaatgtggca acaacagtga taattacacc tatcagtaaa
    13741 ttcgatttat ttgctttaag caatgtccaa atcgttttat aaagcgtcat aacgcacccc
    13801 tctttccatc caagcatttc ctaacgcaaa aaggaaaata aatccgatca accatagaag
    13861 attggtataa aaactagcca aatcagtgta gaaataaagt ttatacaaac tttcgctgat
    13921 caagttgacg agatttaatt gaccaacaat cggaatatga agatcaatcc aatacttcac
    13981 ttgttccgac cccatcatac cagcaaaaaa gctcatcgca atcgtcactg aaatcaagat
    14041 gctatccttt tgcaccagat tcatttttgg tatcaaatta ccaaccaatg tacctaacaa
    14101 aatcgcacac aacgacccca gagcggtaac aatcatcaac cattgccaac gttcaccaaa
    14161 gtctacttga tagataaaac gaaagacagc taacataata taaatctctg tcaaaacaat
    14221 cgtaaagctt gctgccaaat tagcgattga aactaacgat tgctttctag gaatcaagga
    14281 caagcgaata ccatttgacg attgatttgc ttgctgatcc tgtacattgc ggacccccca
    14341 catcgtcccg tagagaatcg ccattccaat cagagtgaag aaataaaaac ttttgaaact
    14401 catattacga cctgcatgaa cttcttgaaa agatacttct tgttcaaaaa cagtcatgat
    14461 gggcgcgaga tcaacgttct ccacttctgc cactttactc gaaattatac caatacgctg
    14521 taaaaattga tccatgacgt ttttcatcaa cgtctgactc attcctgctt gacccacatg
    14581 gagcgaaaca gatgaaccat caatgagata aaaaccagcc aaatcatctg catttagctt
    14641 ttcattcgct tctacttccg atagttcctg tacttcaaat aatggtttat cattgacttc
    14701 gacttgttct aagactgtgg taaaattttc cgaaagcgct gtttgatccg ctttgattac
    14761 aattccagta ttaatcgttt caagattgcc accttgatca atctcgccaa aggccgccat
    14821 aaacatcaat cctaaaagaa ttggaaaggc caatgtccaa aacaacatgg atttatttcg
    14881 cagcagtatt tttgtgcgat atttataaaa atgccaaaac attatttccc ctcctctagt
    14941 ctctcaaatc cttgccagta atctctaaaa agacatcatt caaggacgct tctttggttg
    15001 ctatttttcc gtaactgatc ttttcttctt gtaagattgt aagaatcggc aacaatactg
    15061 tatttagttc agtagttgtt acttggagct gacctttttt ataagcgatt ccatcagaag
    15121 ctattgttcg ctgaatcgct gacaacacat tttctgggag taatgggatg tcgatcgtca
    15181 aacgatccgc cgtccgtacc atatctttta gagattcttt ggttccttta gcaatcacct
    15241 gtccttgatc catgatcacg atctggtcgc aaagaatttc tacttcttcc atgtaatgtg
    15301 ttgtatagac aatggttgct ccttgtcgat tcagctcctt gatactttct aaaattttat
    15361 tccgactttg ggggtctaca gctactgtcg gctcatctaa aaaaattaac cgcggcttgt
    15421 gggcaatccc acatgcgatg ttcaaccgac gtttcaagcc accactaagt tctcctggac
    15481 gaaacgaaac atatttttct aatcccacta attgaatcac ctcttcaatc aaggctttcc
    15541 ttttctcttt ttcggaaaca tataaaccgc aaaaataatc gatattttct tgcacactta
    15601 gttcgtcaaa gaccgcaacg tcttgaggaa cgaccccaat cattgccttg acagcataat
    15661 tttcaggagt cattgttgtc ccgaacaatt taatggttcc tttgtcataa tttaacaaag
    15721 aaagcataca gttgatggca gtcgatttcc cactgccgtt tggacctaac agacctaaga
    15781 cttctccttc ttgtacttgc agatcaaaat gattcaatgc tacaaaatct ccatagcgtt
    15841 tgactaattt atcaatttcg ataatcatca tgaaaaactc cttccaaatt tcttgttgat
    15901 ctgactataa taaagtgcaa gaaaagaaac tagtgacgat tggctaaacc acacatgaca
    15961 tttgtcacaa tgaaggagtc tgctatgcat aaatctcggc tactcaacga aactagcttt
    16021 ctactggtta tctcattgct tttgctggta agtcaaggat tcgttgctat gcaagttatc
    16081 tatttattga tcgccgtttt attcactggt gtcagttatt tactgcctac aaagccaaat
    16141 tggattttcc aagttattct atttctttta tgtgcctttt tacctgatct tgtcctgttt
    16201 ctgccagcga cgatgcgagt gatcgcccct gaagaaagcc accttgatta cttgtttttg
    16261 ttaggttttt tgttgatttc gacggttggt aatcatcccc ccatcaaggt cgcctttgtc
    16321 gtaattttgc tgtttttttc cttttactta cgaaaaaaag atcaacgaac gaccgatctt
    16381 gagaaaaagt acattgtctc caaagatact cattgggaaa aacaacaaga gttatcggaa
    16441 caaaataaag cattggccct gtcacaagaa acacaattag ccttgcaaat tgtggaagaa
    16501 cggaaccgaa ttgcccgaga catccatgac aatgtcggtc atctgctgtc aagtgcgatt
    16561 ttacaaattg gtgcgcttga aatgatcaat caagaaccaa aactaaaaga accattagaa
    16621 aatttaagtg ccaccgttca cactgggatg gatcggattc gtcaaagtgt ccatgacctc
    16681 catgagacat cgatctcttt tcaacactcc ttagagtttc tgatcgctga ttttccttct
    16741 tctgttacga ttgaaggaaa cttgtttgaa ggattgaatg aaacgcagca aaactgcttc
    16801 ttaatggtca tcaaagaagc cttaaccaac agtatcaaac atagtcatgc caaaaccgtt
    16861 gttttacatt ttcgaacgtt gccggccttt tatcgcttgc aaatcacgaa tgatggcacc
    16921 agcccacaaa aaaattctgc tcccggtatt ggtttgaaaa cgatgcgtca acgtgttcaa
    16981 gaacaaaagg gacaagtcca tattttccaa gaaaaccagc aatttcagtt aacgatcatt
    17041 ttaccaaggg agggacacca atgataacag tagtcattat tgatgatgat ccatttgtga
    17101 cgaaatcgtt acaaaccatt ttagaaagca cgaaagagat ccgtgtgtta ggcattggtc
    17161 attgtgccaa ggatgcgcga aacccatcgt cccgatgttt tgctgacgga catccgcatg
    17221 cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc caaagccatc
    17281 cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt ttccatcggt
    17341 gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt caaatccgct
    17401 tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca aatgatgaag
    17461 aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga gcttgcaatc
    17521 atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt gtacttgagt
    17581 gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt acgggatcgc
    17641 acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa cacaaaaaaa
    17701 cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt caattgtccc
    17761 gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca ggattcgttg
    17821 gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt tctagttttg
    17881 attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat tctttttcca
    17941 tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct aatttatctt
    18001 cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca tatcacactg
    18061 aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat ctttaaaatc
    18121 aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca atcatcggat
    18181 ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa acagcaggtt
    18241 ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt tccactgttt
    18301 ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt agcttttctt
    18361 ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt ccgaccaaag
    18421 aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca ttatccatga
    18481 tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa gtgacatagg
    18541 cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc gtgtagagca
    18601 aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc atcttgaatt
    18661 cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta ttccattcgt
    18721 ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc actttatctt
    18781 cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg aatacattca
    18841 attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg gtgtcgttcc
    18901 aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc attttattta
    18961 acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata tcttgtgcac
    19021 ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca atttgataag
    19081 catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt ggcagacgtc
    19141 cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc cgataaatct
    19201 gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt acttgaccag
    19261 gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata ttcgtcggaa
    19321 tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca tcaacaatca
    19381 aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt ccattcagcc
    19441 ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt cgtttagctg
    19501 ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat tttgaaggat
    19561 ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc acagtagttt
    19621 tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct tcagcgatag
    19681 cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc cgaatattct
    19741 tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg cgctggatcg
    19801 aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta gtctcttctt
    19861 gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga tcaggctgga
    19921 tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat gtctccgccg
    19981 gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag atagctgctc
    20041 gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta tctgcacgtt
    20101 tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg atgatatcca
    20161 aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg aacccgtaac
    20221 gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc tcagcaagca
    20281 tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga tcagcaatga
    20341 ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt ttcttcccga
    20401 ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt gttggctttt
    20461 cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc actaattctc
    20521 cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg actacaatct
    20581 ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact ttgtaaaaat
    20641 tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc atttcttctg
    20701 gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt ttcagttcct
    20761 aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca aggctcttca
    20821 gtctctaaaa tcgtcaaact actattgaac aatccaccca tttcacgcaa ctcacttagc
    20881 tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc tacgaataaa
    20941 agtgggccat ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat acgtgtgatt
    21001 gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa atgattacgc
    21061 aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg accttctaaa
    21121 gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg acgagcaagc
    21181 tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga ataaattgct
    21241 ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta actttgtggc
    21301 aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca ttgtgtcttc
    21361 ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt ttattttact
    21421 ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa ttctaaatta
    21481 gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt cgataaattc
    21541 aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc gttttcacgc
    21601 ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg ctcttctcca
    21661 tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa atgtagttta
    21721 cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat atttacatca
    21781 ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa acctagtggc
    21841 tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt tccagtgttt
    21901 gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc tctcatattt
    21961 atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt atcaactcgg
    22021 tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt tatcagaaca
    22081 ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc ctcaactttt
    22141 ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag ttccttttaa
    22201 taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg ctttgattcg
    22261 ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat tttcctctgg
    22321 tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt cagcaaactt
    22381 ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat aaaaagcctg
    22441 acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc catcttctga
    22501 gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa ccgcgttgaa
    22561 tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg cttcggcaga
    22621 ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag cattttgaat
    22681 ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa catctaatag
    22741 cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat acatgccaga
    22801 ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt taaccgcatg
    22861 ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat ttttgcatat
    22921 caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga acagctgatg
    22981 attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc cccctcattt
    23041 ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg atacaatgcc
    23101 tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg cgctgaaaac
    23161 tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt gtgtgacaac
    23221 ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt aagttatgct
    23281 ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga cttcgtctat
    23341 aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa attagggacg
    23401 gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga ggccttagaa
    23461 ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt tgacagtatt
    23521 cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt tgaccgtatc
    23581 ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg gcttaaagaa
    23641 ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt cactgcagag
    23701 aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac caaaatcgtg
    23761 gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa tgcttattgc
    23821 aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata ttgaaataaa
    23881 tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct actgctggat
    23941 caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc gccactgtga
    24001 ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt ttccaaaaac
    24061 ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg gtcaagactt
    24121 cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca agtcgacgaa
    24181 cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc agcgaaatga
    24241 agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt aaagaagcgg
    24301 tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac gctgccttta
    24361 ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa tcgctagcag
    24421 cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac aaatgatcat
    24481 cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt gccacatctt
    24541 cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca gaaatcattg
    24601 cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc tgaccttctt
    24661 gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc ttcagcacgt
    24721 acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt gtcatgatcg
    24781 cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca tcgtctgccg
    24841 acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc aagtcaacag
    24901 cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc atttcatcta
    24961 ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc gctaattctt
    25021 taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat tggtagaatt
    25081 gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga atattatttt
    25141 cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt tcatccaaac
    25201 caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt ctgcggctaa
    25261 aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca gatcataaca
    25321 atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat catcaccgag
    25381 cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc ctgtatgcag
    25441 ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact cagtctgtgc
    25501 cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc gggcgatcgg
    25561 caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta aggcctgata
    25621 ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg caaatccatg
    25681 ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt cgcgtcacca catggattac
    25741 ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca ttgtcccatt
    25801 tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta ccagaacgtg
    25861 ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt catgttctcc
    25921 ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca agacattttc
    25981 tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta acagaccctt
    26041 tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt tctcataatc
    26101 ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga tgccaaaaat
    26161 gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa taaattgagc
    26221 ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct tctgtatttt
    26281 ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc aaagtacgaa
    26341 acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata ctaggatgga
    26401 tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta gaaatcgtca
    26461 atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg gctaccatcg
    26521 tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg tccggtgttt
    26581 catccaaata actgatccga acatccagca agggataact aacactttgt tcccggttcg
    26641 ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg taaaacccta
    26701 aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg tagtgatgga
    26761 acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc tcatgatcat
    26821 ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact tctatagtct
    26881 ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct ttcgctttct
    26941 ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg ggagaataac
    27001 cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa ctgataggca
    27061 gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc ttgatattta
    27121 taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg aatctggatt
    27181 tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat caccagatga
    27241 taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg gagaactttc
    27301 atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac aaactgacac
    27361 atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat tggaatatgc
    27421 cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt gacaaattca
    27481 atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag tttcactttc
    27541 aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg ttccatagct
    27601 gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa gttttttcgt
    27661 actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca aaaaggagtc
    27721 gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa gcaagagtac
    27781 gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca gactaatatt
    27841 tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg gatccgtttc
    27901 gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg tctcgaaaca
    27961 acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg tctgcctcgt
    28021 tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc attaatccaa
    28081 accggaaagc ttacaacaaa acgagctgaa ttccctattg aagaaggact tttagctatt
    28141 gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt aggggatgca
    28201 tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc ttatcgtcct
    28261 gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt cattttcaca
    28321 aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg aaagaatcta
    28381 gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat ctatttattt
    28441 gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca atttatcgaa
    28501 gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc tttggtcaat
    28561 ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa tcttgaggaa
    28621 cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc ggcgcagttt
    28681 caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt cggctgtaat
    28741 gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg cggcgatttg
    28801 aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt ttctgatcaa
    28861 aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta caattatgcc
    28921 tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct ttggagaatc
    28981 acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga acatttacat
    29041 acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc taattaa
//
LOCUS       DADVXU010000024        29096 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.026, whole genome shotgun sequence.
ACCESSION   DADVXU010000024 DADVXU010000000
VERSION     DADVXU010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 29096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.026"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            278..1063
                     /locus_tag="KB953_001036"
     CDS             278..1063
                     /locus_tag="KB953_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBB6866025.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            1076..1927
                     /locus_tag="KB953_001037"
     CDS             1076..1927
                     /locus_tag="KB953_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HBB6866026.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(2051..2503)
                     /locus_tag="KB953_001038"
     CDS             complement(2051..2503)
                     /locus_tag="KB953_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6866027.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(2968..3336)
                     /locus_tag="KB953_001039"
     CDS             complement(2968..3336)
                     /locus_tag="KB953_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HBB6866028.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            3486..3968
                     /locus_tag="KB953_001040"
     CDS             3486..3968
                     /locus_tag="KB953_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HBB6866029.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            4005..5015
                     /locus_tag="KB953_001041"
     CDS             4005..5015
                     /locus_tag="KB953_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HBB6866030.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(5130..5843)
                     /locus_tag="KB953_001042"
     CDS             complement(5130..5843)
                     /locus_tag="KB953_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866031.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(6033..6623)
                     /locus_tag="KB953_001043"
     CDS             complement(6033..6623)
                     /locus_tag="KB953_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HBB6866032.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            7107..7334
                     /locus_tag="KB953_001044"
     CDS             7107..7334
                     /locus_tag="KB953_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBB6866033.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            7350..8516
                     /locus_tag="KB953_001045"
     CDS             7350..8516
                     /locus_tag="KB953_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBB6866034.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNAHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            8750..9793
                     /gene="hrcA"
                     /locus_tag="KB953_001046"
     CDS             8750..9793
                     /gene="hrcA"
                     /locus_tag="KB953_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HBB6866035.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            9887..10450
                     /gene="grpE"
                     /locus_tag="KB953_001047"
     CDS             9887..10450
                     /gene="grpE"
                     /locus_tag="KB953_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBB6866036.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            10501..12330
                     /gene="dnaK"
                     /locus_tag="KB953_001048"
     CDS             10501..12330
                     /gene="dnaK"
                     /locus_tag="KB953_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBB6866037.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            12481..13647
                     /gene="dnaJ"
                     /locus_tag="KB953_001049"
     CDS             12481..13647
                     /gene="dnaJ"
                     /locus_tag="KB953_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBB6866038.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(13729..14394)
                     /locus_tag="KB953_001050"
     CDS             complement(13729..14394)
                     /locus_tag="KB953_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6866039.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            14370..14543
                     /locus_tag="KB953_001051"
     CDS             14370..14543
                     /locus_tag="KB953_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866040.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(14588..15325)
                     /locus_tag="KB953_001052"
     CDS             complement(14588..15325)
                     /locus_tag="KB953_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBB6866041.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(15327..16829)
                     /locus_tag="KB953_001053"
     CDS             complement(15327..16829)
                     /locus_tag="KB953_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HBB6866042.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            17088..19082
                     /gene="uvrB"
                     /locus_tag="KB953_001054"
     CDS             17088..19082
                     /gene="uvrB"
                     /locus_tag="KB953_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HBB6866043.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            19092..21908
                     /gene="uvrA"
                     /locus_tag="KB953_001055"
     CDS             19092..21908
                     /gene="uvrA"
                     /locus_tag="KB953_001055"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBB6866044.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            22171..23436
                     /locus_tag="KB953_001056"
     CDS             22171..23436
                     /locus_tag="KB953_001056"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HBB6866045.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            23440..24171
                     /locus_tag="KB953_001057"
     CDS             23440..24171
                     /locus_tag="KB953_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HBB6866046.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            24306..25190
                     /gene="rapZ"
                     /locus_tag="KB953_001058"
     CDS             24306..25190
                     /gene="rapZ"
                     /locus_tag="KB953_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBB6866047.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            25187..26185
                     /locus_tag="KB953_001059"
     CDS             25187..26185
                     /locus_tag="KB953_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HBB6866048.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            26219..27154
                     /gene="whiA"
                     /locus_tag="KB953_001060"
     CDS             26219..27154
                     /gene="whiA"
                     /locus_tag="KB953_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HBB6866049.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            27697..28347
                     /locus_tag="KB953_001061"
     CDS             27697..28347
                     /locus_tag="KB953_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866050.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            28534..>29096
                     /locus_tag="KB953_001062"
     CDS             28534..>29096
                     /locus_tag="KB953_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HBB6866051.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT         9565 a         4897 c         6176 g         8458 t
ORIGIN      
        1 aaggggctgt gacaaaagtc ttgtcacagc ctttttttcc aaataaatgg tttttcagaa
       61 gtagcttctt cggaaataag ccgaaatttc ttgaagctga ccacttctgt cacaacctct
      121 ttctttatag aaataaaagg acactagcaa ttcatgaaaa cgattgctag agcgtatcat
      181 ttgctttaga ctatatatct tggtgtacac tcactttaag gcggaaataa aagttatccg
      241 taataacagg tttaggtatg aaaaaggagt gggtactatg tacaaggaat tagaaaacaa
      301 agtcgcagta gtaactggtg gctcaaaggg gattggaaca gcgattgctc aacgattcgg
      361 gaaagaaaaa atgaatgtag ttgtcaacta taactcggat aaagaaggag cagaaaaagc
      421 agtcaaagca atcgaagagg caggcggaaa agcagtctct gtacaagcag atgtcagttc
      481 tgaagaagga atacagcgtt tacttgatac ggctttagaa aattttggga cactggacgt
      541 ttgggttaat aatgctggga tggaaaatca gcatccaacc catgaactta gccttgaaga
      601 ttgggaaaga gtgttaaaag tcaatttgac tggcgttttt atcggaacga aagcggcttt
      661 aaattacttt acagagaata ataaaaaagg aaatatcatc aatatgtctt ctgttcatga
      721 acagatccca tggccaacgt ttgctcatta cgctgcttca aaaggcgggg taaaattatt
      781 taccgaaacg gtagcaatgg agtatgcacc gaagggaatc agaatcaata gtataggccc
      841 tggtgctatc cgtacaccta ttaatgcggc aaaatttgat gatcctgaac aaaaagaact
      901 cttggaaagt atgatcccac taggacgcgt aggtgaacca aaagaaatag cggcgacagc
      961 tgcatggttg gcttcagacg aatccagtta tgttacaggt attacactat ttgcagatgg
     1021 tgggatgact ttatatcctt ctttccaagg tggaaaagga taagggtgaa taaaaatggg
     1081 tttattgatt gcattgatcc cggcagttgg ctgggggatc cagccactga ttttgaaaaa
     1141 gattggtggc agaccaacaa atgagatatt aggaactggt attggagcgg tgcttgtcgg
     1201 cttactcgtt caattattcg cttctccagc tggtatttca gtgactacat ttttgattag
     1261 tttactttct ggtgcatttt gggttattgg acaaacagga cagtatacat ctttcaactt
     1321 gattggtgtt tctaaaacga tgccaatttc gactgcatta cagttagtcg gaacctcgtt
     1381 gattggtgtc ttcgcttttg gagaatggtc tggaagtatg gggaaaatca ttggagcagc
     1441 agcaatcatt cttcttgtat ttggttctgc tttaacggca atcagtgatg atcctggaaa
     1501 aaaaggcggt atcacaaaag gaattagtct attagcatca acaagtattg gttattgggt
     1561 atacagtgca ttgcctaaac tagttgatgc aagcggactt tccattttct ttccgcaaat
     1621 gatcggtgtc tttttagggg cagtgattta tgtgattttc cgtaaaccaa ccgcattttc
     1681 tgaagacaag agttggaaag caacgatcgt tggagccatc tttagtgtgt cagcattggc
     1741 ttatattttt tcagcaaatg aaaatggggt tgctactgca tatatcatta cacagctaaa
     1801 cgtagttatc tctacattag gcggtatgta catcctgcat gaaaataaga caccaaaaga
     1861 aaaaaaatta acgattatcg gattattgct gatcgtagta ggaagcatgg tcacggtctt
     1921 tatttgagga taaagatggt aaaatttcat ttattaaaaa aatatttgtt tgaaccaaac
     1981 agtaagtact tataaaaaaa tagcgctaga atttccaaca tctctggaat ttctagcgct
     2041 ttttaattgt ttaagataca agggaaatat ctgtctggat caataatttt tcattgatcc
     2101 attgactaaa gtttacgaca taattgaagt taccaccgat tagtatctcc gcatgattgt
     2161 ctttataaag atcgtattgt aaaagacggt cttttttatt tgcgtaaaca tcgttatggt
     2221 actggactaa ttgttctcgg aattgattga ttgctgtaaa atcacgatat tgaaggttga
     2281 aacgcatcgt gattggtgca tttagcatga atcgctgcat ttcttttgct gcttcctctg
     2341 cttgatctcc gtataggaac tcaaaaagat tacgttcaga aaatttccat tgcccgccaa
     2401 cctttgttcc ttttagtttt ccttcatgaa gataatttct gatagaacgt gtagtaacac
     2461 ctaattgttc ggctaattca tttactgagt aaaaatcttt caatgttcac cctcctatgt
     2521 gaatggttct actgacttag ttgatatttg cttaatcacg aaatgtagaa agaacgattt
     2581 taattaaggt cattgtaaaa atcaagtcag aaaattctaa cttttatttc tctaaagaaa
     2641 ttcgttctct cctaaagtga tattatattt aaatatacct gaaaataatg ggcagtatta
     2701 tgaaagatca ctgatatttt ctcgtatttt ttccttattc aaaagttttg caggaaaatt
     2761 gctgaaaaaa taattttact aagtagaaaa ttcaaagcca ttcaatcagt gatttttttc
     2821 agcacaagaa agactcctta taaaaatagc agatactttg tcaattaagt gtttaaccag
     2881 ggatgataaa aaggatagga ttgaacaaaa cctgtataag gtgatgaata gtttcctcgc
     2941 cagtaaaagg tttattcgta taaagcttta tcgccaaaat ttatcatgac cttttttatc
     3001 ttcaatatta aatgaaatca attgttcaat caattcccag ttgattacac ttttccaagg
     3061 aactttgatg acgttatccg tatatacgta gcctgcttct tggatatttt tttcaagggc
     3121 acgaatcgct gctttttccg gtgcgataga aaaatgattt ttagcaaaac taaatgcaat
     3181 aataaaggta ccatggtcag taaacatagg ttggttccat tttacagttg tgtctagctg
     3241 gggatacgtt tctgttatct tagcaaaaag gtctttgatc ttttgttgat gttctttttc
     3301 tttgatttga ttgatgatcg gctgaaattt ttccattcta aaacgcctcc attaagtggt
     3361 ttgatttatt ttaccagaaa aacaatagtt atgtataaaa aacgaacaaa acacaagtaa
     3421 tgttgtaacc gctatattta tatgataaga tgtctcaaat aataaaatag aaaagagaca
     3481 taaaaatgat tgacttggca aagaaattct tacacgaaaa tatatatttt gagttgtatc
     3541 atcatcgagc catttataca aatgaagatg ctttggtaat aaagaaagaa caaggattta
     3601 caggtacaga gaccaaaagc ttattcttaa aagataaaaa agataataac tacgtatatc
     3661 tgacttttac tacgaaacaa acggatttca aagcattagg gaagatcgtc ggtaaaaggc
     3721 tttctattgt ttcatcagaa cgcatggaag aacaaacggg acaaaagcag ggcgctgttt
     3781 ctccgtttgg ttatgaaaaa cagattccga ttattattga tgaagagctc ttatcaaatg
     3841 aaaaacttgt ttttgcacca ggtagaccag atcaaacgat ggtcatccaa acggttgact
     3901 taaacaaaat catccagcta cttgagatgc cgatattttt gatgccggca aacgaaaaaa
     3961 acaattaacc aagtaaaata gtgcatactt ggaggtgaag caacatgata agttatccat
     4021 tattagaaag aaatgcaacg attggggtta ctgcaccatc ctcaggcgta tcagaggaac
     4081 accattcgtt actcgaatca gctttaaaga gaatggaaga aaaatctttc caagtagtta
     4141 ctggaaagac gctttggacg caagataagg aaaaatcagg tccagcaaga aaacgagcag
     4201 atgaattcaa tcaaatgatg caagatgaaa atatccaaat gatctttccg acttggggag
     4261 gagaattatt gattgagatt ctcgattttg tcgattttga tgctctacaa ccaaaatgga
     4321 tagtaggtta ttcagatatt agtacgttgc tacttgctgt cacattaaaa acagggatag
     4381 ccactgcaca tggaactaac cttattgatt taagaggaga gtacttggat gaaacgacag
     4441 caatgtggga aagtgtactg aaaacaagag aaggagaatc gattctacag caatcttctg
     4501 aaaagtatca aaaagtgtgg ccccatgagc attcatcatc ttctgttttc catctcactg
     4561 aaccaacaaa ttggcaatca gtcagcggag atgcagtaag gatgaaggga cgattattag
     4621 gtggttgtat tgatacgatc aggcatttaa tcggtactcc ttatggagat gtccaatctt
     4681 ttaaaagaaa gtataatgat ggaaagccga tcttgtggtt tctagaaaac tgtgagctat
     4741 cgtcagttga tttaagaaga tcacttgttc agatgaaact ggcaggctgg tttgatggca
     4801 gctcggggat catgttcggt agaagtgcgg cagaggattt gttagattat aaaatgaagg
     4861 atgtatacga agatctgtca aaggaattaa aactaccgat cttgtatgat attgattgtg
     4921 gtcatgtacc gccacaactg acattgatta atggtgccta tgcagaagta gcggtagaag
     4981 atagaaaagc gactgttttg caaacgttta tttaaaaaat tttttcctgc cttggagtct
     5041 atcaaagacc aataaatcta aatagaaaag aaagtgaaag aagctgacaa aatgctgtca
     5101 gcttcttttt gtataacata ttagaaagtc taattatcta taactgtcac atcgattaac
     5161 tgtttcacgt cattgggatt tcctgtagga atagctacta cagatgcatt atcaatgatt
     5221 tcttgagttg tttctctaat gaacgctgga taagatgttt gatctgcagg catttgccaa
     5281 tgtgaaggaa ctggataact agtgattgtc ccgtcatggt tacttgaata cactaaaact
     5341 ccctggtaac tgtatttccc agtgagcata accacatctt tcggatagac tgcactccct
     5401 gcatcataag gggcgatagg tgttcctgca ggataatgaa taacatgtaa ttcgaaatca
     5461 cccgatgtat ctctaaaagt attacctcta atagctagcc aaacacgtgc gtactcaatt
     5521 tgtagatcag aatatcctgc taacggatct attgactgag aagactgtga ggaggttgtg
     5581 tctattgttg aagaagaaat atttgtctca actgtactac tagatgtaga agaagtattg
     5641 ggctctgaga ctttactgct tgttgaagat gtattcgttt cagtagaagc tgatttctga
     5701 gtacttgatt tttcagctga actggtttgt ctgattgaac ttttgtctgt ggtcgcagat
     5761 ggatcactat tttctttact aggagacgta catcctacaa ggaataaaga gaccaacaat
     5821 aaaataaaac ctaacttttt catataaaat acctcattat ttttttaata ccagtatagc
     5881 ataatcctga atttttgaat ccgtaaaacc ttgtctatga atataaatag agtgtatctt
     5941 ctttgataaa tgtcagaaaa agtgtgacaa ataatcatca gtaaaattac ttgtcacact
     6001 attgataagg agtatttaat tgaagagaat gattaggaga aaggatctcc gtacttttta
     6061 tagtaatcat agaatccaag tttgtataga cgaatcgttt tttctcttat ttcttctgtt
     6121 tcaaaatgga ctgcttgaag aatcaagtct gtgaattcta taggagctgt tccattcgca
     6181 gtaatcaaat tacgatcttt cacagcttgt tgttctataa aatcttcttt attctgataa
     6241 ttttccgatt ctaaccaagg aaattgcgca tttccggtgt gtttgtaacc ttgcaataat
     6301 ccgctctgag ctaaataatc aactgcccca caaatagcag ctactggtac gtctgttttt
     6361 aacaatttat cgattatttg atataaatga tgatttttta tgtcccatga attgccaccg
     6421 atcataataa aaaggtcaac tttttcaggt atttgttcga ttaagtaatc gatttttgtt
     6481 tttaatcctc caatggaagt gacctctgat tggatagaag cagttttgtt ttgccatttc
     6541 tcattttggt ttagtgtgct agtaatataa gcaccttccc aatcggcata ttcctctaat
     6601 agaaacgtaa tcgcagtttt cattttcaag ctctccttta ctatctataa caaggacaac
     6661 agttttaacc tacagacatt cattaaaatt gggtagcgac agcgaaaaca acaattctta
     6721 agttttagac agagtataca gatagtcaac ttaattaaat acgaactaaa taaaaatgac
     6781 aggtaatcat ttttgtttac cagcattata acattttgca tacatggatg acaaaagaac
     6841 caattcccac ttgcttgagt acaatattaa aagaaactgg aaggcaaaaa gtaattatag
     6901 aaacgaaaaa taaatcatat cgggcactca gacatgaaaa caaccctaga gatatataac
     6961 taggtaacca gtttaatgaa agaaaaaatc atccaagaag tcgattcttg gattttttag
     7021 tggatttgtt cgccaaatac gtttagtatg cgtatatgag aggttgtaag caaagcgttg
     7081 tggtctgagc acttaaggag gaatctgtga aaatagcttt tcatattttt gcagattttg
     7141 acttaatgcc gaaggtcagc tttgcgatta ccgtttatcg agaggctgta aaagcaccgt
     7201 gtagctccga tgacaataag caagcatttt tagagctagg tttccttgat tctaaatatg
     7261 cggcttattt caatgagttg gtgcttgaac gcgattttaa gaaggtcgaa aacaggcacg
     7321 acttttcgac gtaaggagaa agaatttcaa tgatagaaac aataaatccc gcagaaaaca
     7381 aggtttctgc gtacattcat atcccatttt gcgagcatat ctgctattac tgtgatttta
     7441 ataaagtttt tttagaaggg cagccagtag atgagtatgt ggaaatgctg ctgaatgaaa
     7501 tgaaaataat gctagaaagt catccgacag cgaatttaga aacactttac gtagggggcg
     7561 ggacaccaac ttctttatct gctaaacaac ttgatcgact gttatcaggt gctagagaga
     7621 tcctgccttt tcaaaatggt aatgagttta ctgtagaagc gaatccaggt gatttgaccc
     7681 gtgaaaaact tcaagtaatg aaaaactatg gcgtcaatcg tttatccatg ggagtgcaga
     7741 catttgataa tcgcttattg aaaaaaattg gacgtaaaca caccgcagaa gatgtgtatc
     7801 aaactatgca atttttagaa gcagaaaatt ttaccaatgt aagcatcgat ttgatttatg
     7861 cattaccggg acaaacacta gaaggatatc tggatacttt ggaaaaagct ttggctttgg
     7921 atctgcctca ctattctttg tactcattga tcctcgaaaa caaaacaatg ttcatgaact
     7981 gggtacgaca aggaagactc gaactgcctg atcaggaaac ggaaaccaga atgtttgaag
     8041 agacgattga agcaatggaa aagcatggac gtcaccaata tgaaataagt aactttgggc
     8101 tctccggaca tgaatctcaa cataacctca tgtactggaa caatgcccac tattttgggt
     8161 ttggagctgg tgctagcggt tatcttggag atattcgtta ccgcaacaaa ggaccaatcc
     8221 aacactattt acgcccatta cgagcaaatg aacgcccggt attggaagaa gaaaaattga
     8281 cacgaaaaaa tcaaatagaa gaagaaatgt tcttaggact aagaaaaaaa acagggatta
     8341 ctaaaaaaca tttctatgat cgctatcaaa aaacaattga atcggtgtac ggtgatgtgc
     8401 taagggaatt agagcaagaa gggttacttg ttaatgaaga agaccgtgtt tatttgacac
     8461 caagagggac ctttttgggg aacaatgtgt ttgagcgctt tttgtttgaa aaatagttta
     8521 aagaatattt gttttttatc tgaatttaaa taaaaaaagt tcaaaaatac aataaaggac
     8581 tattgaaacg ttgatataac tggatttaca tcaattattt agaattagca ctcttgtgtt
     8641 tagagtgcta aaaaatggag tctttctctt gacaggagaa gggcttcttg ctatatttat
     8701 aagtggatta gcacttagaa tatatgagtg ctaatgagag gtgaggaaga tgttaacaaa
     8761 gcgacaaaac gacattttac gtctgatcat ccaacactat acgagtagtg gcgtgcccgt
     8821 tggttccaaa acattgatgt ctgagggagt caaggccagc tcagcaacga ttcgcaatga
     8881 tatgaaagca ttagaagatg aaggattgct gttgaagact cactcttctt ctgggcggat
     8941 tccatctgct ttaggctatc gttattacgt ggatcatttg ttaaaacctg ctcgcgtagc
     9001 agaagatgat ttgctgctga ttcgtcagtc tctaggcaga gaatttcatg aaatcaatga
     9061 gattattaag caatctgctg agatattatc tgagctgaca agctatacga ctttttcatt
     9121 gggacctgaa ataaaagacc gccgactgac tggctttcgt atcgtaccct tgaataatcg
     9181 acagattatt gctatcgtcg taacagatat ggggaatgta gaaagccaag tgttcactct
     9241 tcccgaatat atgggaagtc aggaactaga aaaaatggta cagattgtaa acgaccgtct
     9301 agtcggagat cctctgatga cggtgtacca taaacttcgt acggagattc cgatgattct
     9361 tcataaatat ttccaaacga cagaagggat gtctcagtta tttgacaccg ttcttggtca
     9421 tgtttttgaa gataaggttt atgtcagtgg acaaatgaat ctcttggatt atgagccccg
     9481 tcaagatctt gatcagttca aatcgatgtt ttcttttatg aaagactcag acggacttac
     9541 acaaatgatc actccgatgg atagcaatat tcatatcaag attggtgctg aattaggcaa
     9601 tgatttattg cagaatatga gtatgattca agcaagttac gagatagatg gtcatggaag
     9661 aggtacaata gccttgttag ggcctactag tatgccttat tctcggatgt tcggtctagt
     9721 cgatgtttat cgaagagaac tagcagctaa acttgctgat tactaccgtt cgcttgactt
     9781 gtcaaactcg taatgtgaat aacgaagcta tttgcagctg atcgttctct ctttcttagt
     9841 aagagcaatc aaacagctga ctgaataatg aaaggaagac tgtatcgtga aagaaaatca
     9901 agaagaacta gataaagaaa tgacagatgc tcagccagaa ccggaaatcg atgtcgaagc
     9961 tgcagaagat tcgggtattt cagaagctga agcggaagaa ttcgaaacag cgaaattaaa
    10021 agctgagtta gaagaaatgg aagataaata cttgcgggct cgtgctgaaa tcgcaaatat
    10081 ggcaaatcga ggaaaaaatg agcgtgagca attacaaaaa taccgttcac aagatttagc
    10141 caaaaaatta cttccatcaa ttgacaacct ggaacgtgca ttagccacag aagtgtcaga
    10201 tgatcaaggg gcaggtttga aaaaaggcgt ggaaatggtc ttggaaagtt tgcgtaacgc
    10261 actggaagaa gaaggaattg aaaaaatccc tgctaaagga gaagcctttg atcctaatct
    10321 tcatcaagca gtacaaactg ttccagcgac tgaagataca ccagccgata cgattgtaga
    10381 agtactacaa gaaggctaca aattacatga tcgcgtgtta agaccaacca tggtcattgt
    10441 tgcacaataa ttgtgcggat ataaaactta cactttcaat taaagaagga gacgttaatt
    10501 atgagtaaaa ttatcggtat tgacttaggt acgactaact ctgcagtagc agtattggaa
    10561 ggaaacgaag caaaaatcat cgctaaccca gaaggaaacc gtacaacacc ttctgttgta
    10621 tcattcaaaa atggagaaat ccaagtaggg gaagttgcaa aacgtcaagc tgtgacaaac
    10681 ccaaatacga tttcatcaat caaacgccat atcggtgaag caggttacaa agtagatgta
    10741 gaaggtaaat catatacacc acaagaaatc tcagcaatga tccttcaata tatcaaagga
    10801 ttcgcagaag actatttagg tgaaaaagtc gaaaaagcag ttatcactgt gccagcttac
    10861 tttaatgatg cacaacgtca agcaacaaaa gatgctggta agatcgctgg tctagaagta
    10921 gaacgtatcg tcaatgaacc aactgcagct gctcttgcct atggtttaga taaaacagat
    10981 cgcgacgaaa aaatcttagt attcgacctt ggtggcggta cttttgacgt atctatcctt
    11041 gaactaggag acggtgtctt tgatgtattg tcaacagctg gcgacaacca tctaggtggg
    11101 gacgactttg ataacaaaat catcgactac atggtagcag aattcaaaaa agaaaacagc
    11161 attgacttgt ctcaagacaa aatggcttta caacgcttga aagacgctgc tgaaaaagcg
    11221 aaaaaagatt tatctggcgt atcaagcaca caaatcagct tgccatttat cacagcagga
    11281 gaatctggac cattgcattt ggaaatgaca ttgacacgtg caaaatttga tgaattaaca
    11341 gctgacttag tagaacgtac aaaagtacca gtacgtcaag cgctaaaaga tgcaggttta
    11401 tcacaatcag aaatcgacga agttatttta gtcggtggat ctactcgtat ccctgcagtt
    11461 gtagaagcag tacgtaaaga aacaggaaaa gaaccaaaca aatcagttaa ccctgatgaa
    11521 gtagttgcta tgggtgctgc tatccaaggt ggcgtgatca ctggtgatgt gaaagatgtt
    11581 gttttacttg acgttacacc attgtcatta ggtatcgaaa caatgggtgg agtgttcact
    11641 aaattgattg accgcaatac aacgattcca acaagtaaat cacaagtatt ctcaactgcg
    11701 gcagataatc aaccagcagt agacatccat gtattacaag gtgaacgccc aatggctgct
    11761 gacaacaaaa ctctaggaag attccaattg acagatattc cagcagctcc tcgtggtatt
    11821 ccacaaatcg aagtaacatt cgatattgat aaaaacggga ttgtaaatgt atctgcgaaa
    11881 gatctaggta cacaaaaaga acaaaaaatc acgatcaaat cttcttcagg tctaacagac
    11941 gaagaaatcg aacgtatggt aaaagatgca gaagcaaatg cagaagcaga taaagctcgt
    12001 aaagaggaag ttgacctacg taatgatgta gatgcattgc tattctctgt ggacaaaaca
    12061 ttgaaagaat tagaaggcaa agtggacgaa gaagaagtga aaaaagcaga aacagctcgc
    12121 gatgaattga aagccgctgt tgaagcaaac aacatcgaag aaatgaaaac taaacgcgat
    12181 gcattgaacg aaatcgttca aaacttgact gttaaactat acgaacaagc ggcacaacaa
    12241 caagctcaag aaaacccaga agcagctcaa ggcggagcag atgatgtcgt tgatgctgat
    12301 tttgaagaag tcgatggcga cgataaataa tcagttcact gaatttcgag agaaggccaa
    12361 agttgctact ttggcttttt cttttgaaaa atagtttgaa gtatcggttt ttgtttcaaa
    12421 caaaatatga tatgataaca agcgacgtga aaatgatgag aaccaatagg aggaaagcct
    12481 atggcaacaa aacgtgatta ttatgaagtc ttagggctct caaaaggagc atcagaagat
    12541 gaaataaaga aagcttatcg taagctttcg aaaaaatacc atcctgacat caataaagaa
    12601 cctgatgcgg aagaaaaatt caaagaagta tcagaagcat atgagatttt aagtgacccg
    12661 caaaaacgcg ctgcatatga ccaatacggg catgcaggca cagatccaaa ttacggtgct
    12721 ggtggagctg gtggcggatt tggtggattc ggcggcttct ctggtggcgg cttcggtggt
    12781 tttgaagata tcttcgactc tttctttgga ggaggcggtc ggtcagtcga tccaaatgcg
    12841 cctcgtcaag gtgctgattt gcaatatacg gtcgacttgt cttttgaaga agcgattttt
    12901 ggtgtagaaa aagaaatcaa gtataatcgg gaagaaatct gtcatacttg tggtgggaac
    12961 ggtgcaaaac caggcacaca accaactact tgtcataaat gtcatggtgc aggtacgatt
    13021 aatgtagaac gccaaacacc acttggacga gtgatgagcc gtcaaacttg tgatgtttgt
    13081 catgggacag ggaaagaaat caaagaacct tgtccaacct gtcatggctc aggacatgag
    13141 aagaaagcac attcagttaa agtgaatgta ccagctggtg tagaagaagg acaacaaatg
    13201 cgtttagctg gtcaaggaga agctggagaa aacggcggac catttggtga tttgtatgtg
    13261 gtcttccgtg tagaagaaag tgatatcttt gatcgtgaag gttctgaaat ttactatgaa
    13321 ttgcctttaa gttttgtaca agcagctttg ggggatgaag tccaagtacc aactgtccat
    13381 ggagatgtga aattgaagat ccctgctgga acacagacag gtacgaactt ccgtttacgt
    13441 ggaaaaggcg ctccgaagtt acgtggtggt gcaacaggcg accaacatgt caaagtaaaa
    13501 ctgatcacac cgaaaaatct aaatgaacaa caaagagaag cattacgtgc attcgctgaa
    13561 gctggcggca tcaaagttga agaacagcaa gaagacggtt tcttcgataa gtttaaagat
    13621 gcttttggcg ggaaaaagcg gaagtagttt ttctagtaaa aacgctaagt cattaataat
    13681 taaaaaccag acgaacaatc atatgttcgc ctggtttttt tgtagttgtc atgtttgtac
    13741 caacaaaaaa gccagcttta gaatatgata atcgtcatag tttctagggg tataaccagt
    13801 gagagaatgg aacttgttca atcgatattg caaggtgttt ttatgaataa ataattcttc
    13861 tgagcaattt ttgatgcttc cgttattttt actgaagcat gccaaagtct tcttgaacaa
    13921 atgtacttct tcttctgtca ggcttttcaa aatacgtttt acatataatg ttcgttgatc
    13981 tgacgggatt gctggtaaaa cgatcccgag gtctaaatct tcaaaaaagc ggatctcgtt
    14041 cacagattga tggaaagcta tccattcgca aacttgtttg gcatgaagga aagattgcgg
    14101 tgtctcttgc tctgtataac aaagcgaccc aattccaaag tttagctgta actgatacat
    14161 tttattaaac tttgtctgga ttttatcaag caatgatagc aatgaggctc tggaaatccg
    14221 attgactagg ataatgattt gttgtctatg tacagtgagc aaagcctgtc cgtaataaga
    14281 gaagaccctt tcgagcaact ccaataaagg attgatccgt tcttgacaca attgctgatt
    14341 gtcagaaagt tgtcccacaa ctacataata tggagaagaa aaatcaagct caattaagtg
    14401 agggtggtat cgactatgta cttgatatca tcaatgaacc gatggtactt tcttatgcga
    14461 tggaacaggt agagcgattg cttgtttgtg caggaaaaca agcgatgcgg ctgatacgat
    14521 taggtgagaa aataagaaga taaaaataca cggtcccttc atggcgaccg tgcatttttg
    14581 gtttttatta aatatttaat actttattca agaaatcttt tgtccgttca ttttgcggat
    14641 tttcaaagat ttgttgaggc gttccatctt ctaaaaagtt tccgtcgtca atgaacatga
    14701 cgcgattggc cacttcttta gcgaacccca tttcgtgcgt aacgataacc atcgtcatac
    14761 cttgttctgc taactttttc atgacgttca agacatcacc gaccatttca ggatcgagtg
    14821 cagaagtagg ctcatcaaaa agcatgatgt cagggttcat cgctagagca cgagcaatag
    14881 ccacacgctg tttttgtcca ccagaaagca tttcaggata catatctttt ttatcttcta
    14941 gaccaactgt ctgcagtaat ttgattgctt ttttctctgc ttcttctcta gattctttct
    15001 tcaggtccat cggtgctaaa atgatgtttt ccaaaataga aagatgaggg aataggttaa
    15061 agtgttggaa taccattccg atatgttgtc tcaccaagtt gatatctgtg cttttatcca
    15121 tcaagtgggc gccgtcaata ataatttcac cgtcagaagg ttcttctaaa cgattcaagc
    15181 aacgcaagaa tgtacttttt cctgaacctg aagggccaat gatacagacg acatctcctt
    15241 cgttgatgga tacattgatg tcgtttaata ctgtgttatc gccgtatttt ttgactaaat
    15301 gctcaactaa gattttttct gccatcttac ttcaccttct tttctaaaac ttttgctaat
    15361 tttgtcaatg ctgtgatcaa aatcaagtac atgatcgcaa tgatcaagta aacatacgta
    15421 ctttgtgttg tacgtgcaac aatgatctta ccagtttgca gcaattcgac tacaccgatt
    15481 gcggaaatga tcgttgtgtc ttttaatgag ataacgaact gattgacgaa tgatgggatc
    15541 atgatgcgta tcgcttgcgg caaaatgatt ttttgcattg tccgattata agaaagacct
    15601 aaacttctgg aagcttccat ttgtccgaca ggaacggcgt tgatcccgcc tcgaacaatc
    15661 tctgcgatat aagcacttgc gttaagagtc aaggtgataa caccagcaag gaagtcagga
    15721 atcgtaaatc caacgatacc aggcaaccca aagaaaataa agaacgcaag aaccatcatt
    15781 ggaattcctc ggatgatatc gacataaata ctagcaattg tgcgaagagc gcgtattggt
    15841 gctacactga ataatccaaa aatcacaccg attactatag ccaaagcaaa ggagatcaaa
    15901 gttaggagaa ttgttctcca aagtcctttc aacaatgctt gatagttgtt ttggataagt
    15961 ccaagaaatg ttgattcatc agcagtggaa gactcactgg aagtttctga accatccgct
    16021 acatattctt taataatctt atcgtattcg cctgttcttt ttaattcttt caatccttca
    16081 ttgaacatat taagaagctc aggattttgt ccttttttaa cggcaaatgc atattttccg
    16141 ccagattctt ttttgatcgg tgtttccaat tcttgtcctt gtttgatcgc ataaccgatt
    16201 accggataat catccatcat tgcatcgatt gcgccaactt ttaacccgtc gtacaattga
    16261 tcagcagcat caaaatattt gatagaatag ccatattcag attcatggtc gcttaaaaat
    16321 tcagcacttt cggtaccaac ttttgctccg actgtttttc ctttcaaatc ttcataagac
    16381 ttgatcgaag tatctccttt tttgacagct aattggatcc cgctatcaaa gtaagaatca
    16441 gaaaaatcat agcttttctt tcgttcatct gttacagtca ttccggcaat cattccgtct
    16501 gcttgtcctg attccaaagc ttgcaaagca gcagagaaac cacggtagtc cattcggatg
    16561 ttaaatcctt gcagtttggc gatgttttcc atcaatttta catcgatgcc ttcgtactcg
    16621 ccctttgcgt tttgaaattc aaatggagca aaagcagagt cactggcaat tacgtatacg
    16681 tcttttttgg gctctatttt ttgcattgtc tcgcttgtgc ttgactctgc cgttgtagtg
    16741 ttgtcagctt gtgcaacatg accaaacgca agtgtcacaa gtcctgtcat taagaaaaca
    16801 aatgagtgaa ataaatgctt tcttttcata atcaacctcc taaatttcca atattttatt
    16861 attataggcg aaaagtgctg aatcagacaa gactgtaagc tcgaaaaaat gaaaaaaatg
    16921 cgagtttcta acattttatg ttataaaaca taacatgtat aataagataa aaaagtggcg
    16981 aaagtatgtt cggtgtggta taatttttta gtaaggaatg cctttgcttt ccttttcttt
    17041 ttattggtcg taaaatgaaa aggaatgaat gaaaaggagg acgaagaatg attgagagag
    17101 atacctcccg acactttgag cttgtctcga aataccaacc ggctggtgat cagcctgaag
    17161 cgattaacca attagtggat ggcgtagtcg gcgggaaaaa agcacaaatc ttattaggtg
    17221 cgactggaac agggaaaaca tatacgattt ccaatcttat tgagaaagtg aataaaccaa
    17281 cactgattat tgcccataac aaaacattgg ctggtcagct gtacggggaa ttcaaggaat
    17341 ttttccctaa caatgctgtt gaatattttg tttcttatta tgattattat cagcctgaag
    17401 cttatgtgcc ttcaagcgat acgtatatcg aaaaagattc aagtgtaaat gatgagatcg
    17461 acaaattacg acattcggct actagttcat tattagaaag aaatgatgtg atcgtcattg
    17521 catccgtttc atgtattttt ggattaggct cgccttttga gtaccaaaaa caagtagttt
    17581 cgattcgaca gggagcagaa ctagatcgca atcagttgat tcgtgactta gtcagtatcc
    17641 aattcgaaag aaatgatata gattttcagc gcggtcgttt tcgagtgcgc ggtgatgtcg
    17701 tggagatttt tccggcttca agagatgaac gtgccttacg tgtggagttt tttggagatg
    17761 aggtcgaacg tatccgagaa gtcaatgcac tgacaggtga agttttagga gaaacggaac
    17821 acgtagcaat cttcccggcg actcactttg tgacgaatga tgaacatatg gagcatgccg
    17881 tagcgaatat caaagcggaa ttagaacagc gtttaactgt cttgcgaaat gaaaacaaac
    17941 ttctggaagc tcaacgttta gaacagcgaa ccaattacga tattgaaatg atgttagaaa
    18001 tgggctacac gtcgggaatt gaaaattatt ctcggcatat ggacggacgt aaagaaggtg
    18061 aaccgcctta tacgctattg gatttctttc ctgaagattt tttgatcgtg gcggacgaat
    18121 cgcacgtaac catgcctcaa atcagaggaa tgtataatgg tgaccgagcg agaaaacaga
    18181 tgctagtaga ttacggtttc cgtttgccat ctgctttaga caatcggcca cttcggttag
    18241 aagagtttga aaaacatgtc aatcagatca tttatgtctc tgcgacgcca ggaccatatg
    18301 aacatgaaca aacagatacc gttatccaac aaatcatccg tccgacaggg ctgcttgacc
    18361 cagtgatcga agttcgtcca atcatgggac aaatcgatga tctagtaggc gaaataaatg
    18421 aacgagtcga aaaagaccag cgggtgtttg taacgacatt gacgaaaaaa atggcagaag
    18481 acctcacaga ttatttcaaa gaattaggga tcaaagtcaa atatttgcat agtgatatca
    18541 aaacgctgga acgaacagaa atcattcgag atcttcgatt aggtgaattt gatgtgttag
    18601 ttggaatcaa tttgcttcgt gaaggattag acgtacctga agtttccctt gttgctatct
    18661 tggatgcgga taaagaaggc tttttacgta gtgaacgttc cttagtccaa acgattggac
    18721 gagctgctcg gaatgaagaa ggtaaagtaa tcatgtacgc agacaaagta acagattcta
    18781 tgcgtctagc gatggatgaa acgtcaagac gtcgtacgat tcagcagaag tacaatgaag
    18841 aacatgggat cgttccaaaa acgatcatta aagaaatccg agatttgatt tctattacga
    18901 aagaatcgga agatgataca aaagaagcgg tacaagtctc gtacgaagaa atgacgaagg
    18961 aagaaaaaga cacgttattg atgaaattag aaaaagaaat gaaagatgcg gcaaaagctt
    19021 tggattttga aacagccgcg aatgtgcgag atatgatttt agaattaaaa gcttcaaagt
    19081 aggaggacaa catggctaac gataaaatta cgattcatgg tgcacgtgcc cataacttga
    19141 aaaatataga tgtaacgatt ccacgagata aattcgtggt cgttacaggt ttgtccggtt
    19201 ctggaaaaag ttccctagca tttgatacgc tgtacgctga agggcagcgg agatacgtag
    19261 agagcctttc tgcttatgct cgtcagtttt taggacaaat ggataagccg gacgtagata
    19321 gcatcgacgg attgagtccg gcgatttcta ttgatcagaa aacaacaagt aaaaatcctc
    19381 ggtcgacagt aggtactgtg acggaaatca atgactattt acgcttgttg tttgcacgtg
    19441 taggacatcc gatttgtccc aacgatcata tcgagattac cagtcaatca gtagatcaaa
    19501 tggcagatca agtaatggct ttgcctgagc gcacaaagat ccaagtgttg gcaccgatcg
    19561 tggtaaagaa aaaaggacaa cataaaaaaa tattcgaacg aatccaaaaa gagggatacg
    19621 tccgcgttcg agtcgatggt gagatctatg atttaagtga agtgcctgag ctggagaaaa
    19681 acaaaaaaca tgatatcgcg atcgtcatcg accggatcat tgtgaaagaa gggattcgtt
    19741 ctcgcctatt tgattcttta gaggcggccc tgcgattagc agacggctat gctctggttg
    19801 acgtgatcgg tgaagaagaa atgttgttta gcgaacacta tgcctgtcct tattgcggat
    19861 ttactgttgg cgaactagaa ccaagactgt tctcattcaa tgcgcctttt ggagcttgtc
    19921 cagactgtga tggtttaggt gtcaaattag aagtcgatca agatttagtg attccagata
    19981 gaacgaagac acttagtcaa ggtgctatcg ttccatggaa tccaatcagc tctcagtatt
    20041 atcctcaaat gttagcgcag gcttgtgaaa gtttcggtat cgatttagat acaccattcg
    20101 aagaattacc ggaagagcat caggagatca tcttgaatgg gacagatgat cttttccatt
    20161 tccattatga aaatgatttt ggtggcgttc gcgatgtaga aacgactttc gaaggggtat
    20221 tgaagaatat cgagcgtcgt tatcatgata cgaacagtga tttcacgcgt gagcaaatgc
    20281 gtctttatat gacagaatta acttgtcaga catgtaaagg ttatcgattg aatccccaag
    20341 ctctatctgt tcaaatcaat ggaacgaata tcgggcaaac aaatgagttg tctattcaaa
    20401 aagcagtaga tttcttctca aacttaacgt tatctgaaca agagaaagta attgccaaac
    20461 cgattttaaa agaagtaaat gacagacttt cattcttgga aaatgtggga ctagactatc
    20521 taactttgag tcgcgcttcc ggaacattat ctgggggaga agcgcagcga atcagattgg
    20581 ctacacaaat tggatcgaat ctttctggag ttttgtatat tttagatgaa ccatcgatcg
    20641 gattgcatca acgcgacaac gatcgactga ttggctcatt aaagaaaatg cgtgatcttg
    20701 gcaacaccct cgttgtagtc gaacatgatg aagatacgat gtgggcagca gattatttga
    20761 ttgacgtcgg acctggggcc ggagaacaag gcggacaaat cgtggctgca ggcactcctg
    20821 aagaagtagc taatgatgaa aattctttga cagggaaata tctttcaggg aaaaaatcaa
    20881 tccctgtacc aaaagaacgc cgtaaaggta atgggaaagc aatcaagatc actggtgctt
    20941 cagaaaacaa tctgaaaaat attagtgttg aatttccatt aggagaattt gtagcagtca
    21001 caggtgtgtc tggttctgga aagagtacat tggtcaatag tatcttaaag aaatcgttag
    21061 cgcaaaaatt aaataagaat tctgctaagc caggtaaatt caagacaatt tccggctacg
    21121 aaagtatcga aaagatcatc gatatcgatc aaagcccaat cggccggacg ccgagaagta
    21181 atccagcgac ttatacaagt gtatttgatg atatccgtgg gttatttgct caaacgaacg
    21241 aggcaaaaat gcggggttat aagaaagggc gttttagttt caacgtaaaa ggcggtcgtt
    21301 gtgaagcttg tcgcggggat ggaattatta agatcgaaat gcactttttg cctgatgtct
    21361 atgttccttg tgaagtatgt catggtaaac gatataattc tgaaacatta gaagtgcatt
    21421 acaaaggaaa aagcattgct gatattttgg aaatgacagt agaggatgct gtagaattct
    21481 tcaagcacat tccaaagatt catcgcaaac tgcaaacgat tgttgatgtt ggcttaggtt
    21541 atgtgacgat ggggcaacca gcaacgacat tgtccggtgg tgaggcacaa cggatgaaac
    21601 ttgccagtga attgcacaaa atctctaatg gaaagaattt ctatatacta gatgaaccaa
    21661 cgacaggact tcatagcgat gacatcgccc gcttgttgca tgtattacaa agattagtag
    21721 atgctggtaa cacagtttta gtgattgaac acaatctaga tgtaatcaaa acagcagatt
    21781 atatcattga tttaggacca gaaggtggag aaggtggagg aacgatcctt gcgactggaa
    21841 caccagaaga aatcattaac gtaaaagaaa gttatacagg tcactatttg aaaaaaataa
    21901 tggtataaag aggatagata gctgagatag ttaaaaaaac ataaaaaaat gaatttttgc
    21961 ttgttttatt aatatcagtg ttctagaatc attacatggt agttctttct acactgattg
    22021 gtatagataa aaacttccta taagttcaat ccgttcaaaa aagaattgag caatcggagg
    22081 actttccttt gattgctttt ttctttatgg tatgatattc cttgtgtagt tggaaaagaa
    22141 aaacaagaga ttatgagtaa gtgagagata atgatgaaca gtattgaaaa tgaagaattt
    22201 atccctatat tattaggaag cgacatgaac gtttatggta tggcacgttc ttttaatgaa
    22261 gcatacggca aaatctgcca agcatatgct tcggatcagt tagcccctac ccgctacagt
    22321 aaaattgtca atgtagaagt tattccagga tttgataaag accctgtatt tatcgagacg
    22381 atgcttcgtt tggcaaaaga aagatattct gataagagta aaaaatatct tttgattgct
    22441 tgtggtgatg gatatgcaga attgatctcc caacataaac aagagctgtc agaatatttt
    22501 atctgtccat atattgatta ttctttattt gaaaggctga tcaataaagt aagcttttat
    22561 gaagtttgtg aagaatatga tcttccgtat cctaaaacat tgatcgtcag agaagaaatg
    22621 ttagtaaatg gacatttgga acaggaattg ccttttgaat tcccagttgc tttgaaacct
    22681 gcaaacagtg tggagtatct ttctgtccaa tttgaaggaa gaaaaaaagc ttttatttta
    22741 gagactagag aagagttcga tttaatttta ggccggatat acgaagccgg ttataaaagt
    22801 gaaatgattg tacaagattt tatcccgggt gatgatagta atatgcgtgt gctaaatgcg
    22861 tatgtagatg aagatcatca agttcggatg atgtgccttg gtcatccact attagaagat
    22921 cctacaccag catcgattgg taattacgtt gtgatcatgc cagattataa cgaaaaaatc
    22981 tatcaaacca ttaaggcatt tttggaaaaa atcgaatata caggctttgc caatttcgat
    23041 atgaagtatg atcctagaga tggagaatat aaattatttg aaatcaattt gagacaaggt
    23101 cgtagcagtt tctttgtgac attgaacggc ttgaatctgg ctcgttttgt cacagaagat
    23161 cgagtgttca acaaaccctt tgttgaaacg acctatggaa cgaatcaatc agataaagca
    23221 agattatgga tgggtgtgcc caaaaaaatc tttttagaat atgctcggga aaatgaagac
    23281 aaaaaactag ctgagcaaat gatcaaagaa aatcgttacg gtactactgt attttatgaa
    23341 aaagaccgat caatcaagcg ctggttgttg atgaaatata tgttccataa ctatattcca
    23401 agattcaaaa agtattttca tgtgaaagaa ggctagttca tggagaattt tttcagtatt
    23461 ttaggcggaa tgggcacgat ggcgacagaa agttttgttc gcttgatcaa ccaccgaacg
    23521 aaagctacaa aagatcaaga atatttgaat tatgtcttat tcaatcatgc aacagttcct
    23581 gatcgtacag cttatatttt agatcgatca gaagaaaatc caatgccgtt tctgctggat
    23641 gatattgaga aacaaaattt attgcgacca aacttcattg tgttgacttg taacaccgca
    23701 cactactttt ttgaagagtt acaagcggca acggatattc ctatcttgca tatgccaaga
    23761 gaagcagcaa atgaacttgt ccgtcagcat acgacaggga gagtagcgat tctaggaaca
    23821 gaaggaagta tgaaagctgg aatctatgaa cgcgaagtca aaaatcttgg tttcgaaaca
    23881 gtgattcctg atactgcgct tcaagaaaaa atcaattacc tgatctatca tgagatcaag
    23941 gaatcggatt atttgaatca agagctctat tacgaaatct tagaggaagc ggtagagcgc
    24001 ctaaactgtg aaaaagtcat cctaggttgc acagaattat ctttgatgca tgaatttgct
    24061 gaagataatc attacccagt gatcgatgct caatctattt tagcggatcg aacgatcgaa
    24121 cgtgcattag ctgaaagaag cgaggcattg gatacagcat cggaaaagta aaaaaattgg
    24181 atttggggca aatatcctaa aagatatttg gtccaaatct ttttgtaaaa gaaaaagaaa
    24241 actggtaatg atacgcccgg gatgatataa ttttattatg cacaatgtga gaggggttcg
    24301 taaatatgac agataatttg caattagtca tcataacagg tatgagtggt gcagggaaaa
    24361 cagtagctat ccaaagtttt gaagatatgg gctatttttg tatcgataat atgccgcctc
    24421 gattgattcc aaaattctgg gaattgatca aagaatcagg taaagtcaca aaaatggctt
    24481 taggagtgga tttgcgttct cgttccttct ttgaagaaat ccaaaacatg ctgatcgaaa
    24541 tcgaaaatac gaacttcatc gacacttcta tcttattttt agatgcttca gacgaagaac
    24601 tagtggctcg ctacaaagaa acaagacgag cacatccaat ggcgatggac ggtttgatca
    24661 ctgaagggat acgtaaagaa cgggcgatac tagacgactt gaaagcccaa gcttccgtga
    24721 tcattgatac gacaacattg acaccacgac aattacgtga aaaaatcaat gagtcattca
    24781 aatcaaaggg agaccaagga tttcggatcg aatttgtatc atttggtttt aaatacgggt
    24841 tgccgatcga ttcggatatt gtgatggatg tgcgtttttt gcccaaccct cattatatcc
    24901 cggaactaag accattgacc ggaaaagatc agccagtcta tgattatgtg atgtcctttg
    24961 atgaaacgca atcattctac cataaatttt tggatcttct ggaaactgta ttgcctggat
    25021 acattaaaga aggaaaaagc agcttgacag ttgcaatcgg ctgtacaggc ggtcaacacc
    25081 gttctgtggc attgactgaa cgtttggcaa atgctttagg gaaaaactat aaagtcaatg
    25141 tgacacatcg ggacaaggat aaaaggaaag agacggtgaa ccggtcgtga aaatgaaaac
    25201 ctaccggata agaaaaccaa agatcgtagt ggtcggcggt ggcactggtc tgccagttat
    25261 cctgaaaagt ttacgaaatc aaggagcaga tatcacagca gtagtgactg ttgcagatga
    25321 tggtggaagc agcggtgcaa tcagagaatc gatcgctatg gctcctcctg gtgacttgag
    25381 aaatgtctta gtggcgttat cggatatgcc acaattctat gaagatattt tccaataccg
    25441 tttcaaaaag gaagatcagt ttttagccaa tcatacgatc ggtaatctga tcattgccgc
    25501 agtttccgag atgcggggaa gcacttatga ggcgattcaa ttattagcaa aaatgatgca
    25561 tgtagatgga catgtttatc cttcttcaga aactcctttg accttgcatg ctgtttttaa
    25621 agacggaacg actgcagtag gagaatctaa aatcgcgatc aaccgtaaaa ccattgaccg
    25681 tgtgtatgtc tgcaacacaa atgataaaag tgaagccaaa gcggctcgaa aagtcgtttc
    25741 agcaattatg gacgccgaca tggtcgtttt gggacccgga agtttattca ccagtatcct
    25801 tccaaattta gtgataccag aaatcggcga agcaatgctc aagacaactg ctgaaaccgt
    25861 ctatatctgt aatatcatga cccaaaaagg ggaaacggaa catttcacag atgccgatca
    25921 tatacgtgtt ttacacaagc atttgaatca gccttttatc gatacagtat tggtcaatac
    25981 ggaaaaagtg cctgaagatt atatggaccc agagatctat gatgagtatc tagtacaagt
    26041 cgcacacgac ttcaacggat tgagaaatga aggatgtcga gtgatttcca ccgacttttt
    26101 agaattgaaa gatggtggtg tcttccacga tggagacaaa gtagtagaag aattgttccg
    26161 tttagttttc ggttcaaaat attaagcaat ttattgttgg agaaaggagg agaataggat
    26221 gtcatttgct gcagatgtga agaaagaact aactggtttg gaagttcatc gagaacatgc
    26281 gaaagcggaa ttagctgctt tgctgcgaat gaacggctct ttaggtattg tgaaccagca
    26341 gtttattttg aacgtccaaa cagaaaatgc tgcaattgcc agacgtatct attctctttt
    26401 gaaagatcat tatcaggtac gaagtgagct actcgttcgt aaaaaaatga aattaaaaaa
    26461 gaacaatgtc tatatcgtcc ggttgaagca aggcacaaaa gatgtgttga tggatcttga
    26521 tatcatggat ggtatgatgt tcaatggaca cgtttcggac gagattatgg gaaattcgca
    26581 aaaaatgcgt tcttacttac gtggtgcttt tatggcctca ggttcagtca ataatccgga
    26641 aacgagtcgt tatcatttgg aaatattttc tatttatgaa cagcataacc aagacatttg
    26701 cgatatgtta aattattacg gtctacatgc tcgaacgctt gaacggcgca atggctatat
    26761 ttcgtattta aaaggagcag aaaaaattgc tgatttctta acattgattg gtgcaaccaa
    26821 ctcaatgctc cgatttgaag atgttcgaat cgtacgggat atgagaaatt cggtcaatcg
    26881 tctagtcaat tgtgagacag caaatctgaa caagacgatt gatgcggctt ctaaacaaat
    26941 cgaaaacatc gaattgatcg aagcaaaggt cggtctacat gcccttcctg aaaaacttca
    27001 ggaaatcgca gaacttcggc ttcaacatcc agaggtcagc ttgaaagaat taggggaaat
    27061 gattccctca ggtgcaatat caaaatcggg aattaatcac cgaattcgca aaataaatga
    27121 atttgcggaa aaactgcgtg aagaagcagt ttaatccaaa gaaaaaactg atacatgaag
    27181 caggagatgc gaatgtatca gtttttttta aaagctatac ttgcttttct tccgtcaaac
    27241 tataattcca actttactgg agtaacataa tgttgaaagg cttctattgt tttttaatac
    27301 aatcgtataa aaagcgggga gacaactgaa tgaagatttt agttttatac ataaactttt
    27361 gcttaatata gaagtcctga tatagatagg atcaagtatt tatctataac tggagattaa
    27421 ttaaaaactg gaaggaactg ctcgatcaac cttgagaact gtttttctta cttctggtca
    27481 agattctcgt ggttggtgga taaggatggt tgataagttc attggattag ctagtcgaaa
    27541 acaaccaggt gtaattaatg tagaagtttt aacaagggga tggagtgatt tattcagtga
    27601 taatcataag ttcataaata gtaattggga tgtatggttg gtatacgtta tacctgaact
    27661 tgaactatca agatttggtt tagaatttga gaatcgatgt ttataaaggg agggcaaaaa
    27721 atgtgctttt ttacaacttt tactatcatt atgattgcag tactttttat ttttctagat
    27781 atagcaaagc gaaatacagc atttttatta tatcgggttt tacttcgagc aggtctcata
    27841 acatttatat cgatcgttgg attttttcta ttcacagtta ttgtatttat ttggagaact
    27901 acagcaccac cattgccaga gattacatat ggagaatttc cgtttagtct tgaatatgaa
    27961 atagatgagg aaagaatttt gattgaagat acggtgattg cagaatttat aggaagtttt
    28021 agagggaatg tgacaactca acctagacga agttggagta taaaactttt aaataatagt
    28081 ggagagttga aagcatttca ttatgaattc ttaataatag aaagtgaaga tgtaacaatc
    28141 actttaagta gtggctctgc tggatatttt atgaatgatg gtggaagcaa gccacagctc
    28201 acagtccgtg atcgtattgc aggttttatg catttttctc actatgactt agaagaagct
    28261 cgtaaaatac ttgcagaata tgggatcaca ttaatcaatt gggaatatgc gccaccgata
    28321 agaaattttt tcaatgatta ttattagtct tataaaaaac agaatgtaaa atttatgtgc
    28381 gcgagtattt agttttccat tttttgtttg atagatatat agcattgttg aagcatggga
    28441 tagtggtgcc aattttatca atgaggttgg tcaggcagtt aacataattg gtgatgctat
    28501 aaatccattt aattggaatt ggaggaatta ggtgtggaaa cagtgagatt atggatattc
    28561 atgaaaaatt ttcgttcggc tttgagatat gttattctgg aatcaaaatc aggttattat
    28621 ctgattgaca cagatcacta tactttttgg ggaggctacc tttttccgat ttttaattgg
    28681 ataatccccc ataaggttat gaaaattaac ttgcctgcag aagaaataaa tagattaata
    28741 ctcaacaaag atttacaaag gaaaagagag aaagaacatt ctaatgttgc ttggataggt
    28801 ccagcatttg cagtagtgac ttcttcaatt gtcggtccta tgctagagag tatttatagt
    28861 gatttttcta ttggaatacg ttatttattt gttactttaa gtatagctgt tcttttgtgc
    28921 ataaagggtt atgttagcaa gaagaataaa gatattttaa cactaatcgg tgtaaacaat
    28981 ttatctagta ctaaaattat actgtttcct gaatctataa aagaaggatc aaggttgttg
    29041 ataattttta taatgcttat actgttttct gttttatttg gggtgatgtt tataga
//
LOCUS       DADVXU010000025        28139 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.027, whole genome shotgun sequence.
ACCESSION   DADVXU010000025 DADVXU010000000
VERSION     DADVXU010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 28139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.027"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(96..575)
                     /locus_tag="KB953_001063"
     CDS             complement(96..575)
                     /locus_tag="KB953_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBB6866052.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(648..932)
                     /locus_tag="KB953_001064"
     CDS             complement(648..932)
                     /locus_tag="KB953_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866053.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(1010..1204)
                     /gene="ssrS"
                     /locus_tag="KB953_001065"
     ncRNA           complement(1010..1204)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="KB953_001065"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(1276..2562)
                     /locus_tag="KB953_001066"
     CDS             complement(1276..2562)
                     /locus_tag="KB953_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HBB6866054.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            2700..3371
                     /locus_tag="KB953_001067"
     CDS             2700..3371
                     /locus_tag="KB953_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HBB6866055.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            3337..3825
                     /locus_tag="KB953_001068"
     CDS             3337..3825
                     /locus_tag="KB953_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HBB6866056.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            3838..4785
                     /gene="prmA"
                     /locus_tag="KB953_001069"
     CDS             3838..4785
                     /gene="prmA"
                     /locus_tag="KB953_001069"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBB6866057.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            4786..5550
                     /locus_tag="KB953_001070"
     CDS             4786..5550
                     /locus_tag="KB953_001070"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBB6866058.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            5783..7996
                     /locus_tag="KB953_001071"
     CDS             5783..7996
                     /locus_tag="KB953_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HBB6866059.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            8007..8453
                     /locus_tag="KB953_001072"
     CDS             8007..8453
                     /locus_tag="KB953_001072"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBB6866060.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            8624..9883
                     /locus_tag="KB953_001073"
     CDS             8624..9883
                     /locus_tag="KB953_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HBB6866061.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(9972..10919)
                     /locus_tag="KB953_001074"
     CDS             complement(9972..10919)
                     /locus_tag="KB953_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBB6866062.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            11313..12605
                     /locus_tag="KB953_001075"
     CDS             11313..12605
                     /locus_tag="KB953_001075"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBB6866063.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            12620..14389
                     /gene="aspS"
                     /locus_tag="KB953_001076"
     CDS             12620..14389
                     /gene="aspS"
                     /locus_tag="KB953_001076"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBB6866064.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            14498..15373
                     /locus_tag="KB953_001077"
     CDS             14498..15373
                     /locus_tag="KB953_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBB6866065.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            15639..17522
                     /locus_tag="KB953_001078"
     CDS             15639..17522
                     /locus_tag="KB953_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBB6866066.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(17597..17881)
                     /locus_tag="KB953_001079"
     CDS             complement(17597..17881)
                     /locus_tag="KB953_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBB6866067.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(17871..18605)
                     /locus_tag="KB953_001080"
     CDS             complement(17871..18605)
                     /locus_tag="KB953_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HBB6866068.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            18734..19357
                     /locus_tag="KB953_001081"
     CDS             18734..19357
                     /locus_tag="KB953_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBB6866069.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            19549..20670
                     /locus_tag="KB953_001082"
     CDS             19549..20670
                     /locus_tag="KB953_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HBB6866070.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            20677..23805
                     /locus_tag="KB953_001083"
     CDS             20677..23805
                     /locus_tag="KB953_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HBB6866071.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            23824..27018
                     /locus_tag="KB953_001084"
     CDS             23824..27018
                     /locus_tag="KB953_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HBB6866072.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(27164..27871)
                     /locus_tag="KB953_001085"
     CDS             complement(27164..27871)
                     /locus_tag="KB953_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBB6866073.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT         9371 a         5020 c         5989 g         7759 t
ORIGIN      
        1 attataaaga ccgatgagtg cttgccgcgt cttttgtttt tatcacggtt ttttgtgcaa
       61 aaaaacctgc cgatagttgg caggtcgtct acgtgttaag ctttggtgtg gtctttagct
      121 ggcaattggt tttctaaatc agttcggaca acaagcacat cgcaagctgc gtttctaata
      181 acatattcag aaacagaacc aataaataaa cgttctacag cattcaatcc tgtggctcct
      241 aacataatca aatctacttg attatcttct gggatttctc tggcaataat ttgttttggt
      301 gagccatatt ctacgacaga ggttacatta ttcaagccag ctttcttcgc attgctttca
      361 taatctgcca atgtttgctt tgccatttca gtagcttgtt ctgcaagcat tccatcaaag
      421 gatgaaacgg tttggaaaga acgtgtgtca atgacatgtg ctaatagaag ttctccgtta
      481 tttcggatag ctacattgac cgccttttta aaagctaatt cagcttcatc agaaccatct
      541 acggcaacca tgattttttt gtattgttgt aacataagca ttcactcctc aagtcaatcg
      601 tattgtactc ttttattgta gaacacttta gggaaaaaga gaattattta ctgctttggt
      661 tcagcaattt tttttgcaaa tcggatactg aaggtggcag aaacaataat tacgatcagt
      721 aaaaagacca aggcaataaa gcgttgattg aaaaaagctg ttaaaatacc cattacgcct
      781 aaaacagcat atgcagctcc gtaaaattgt aggaatcctt gatttttatc atttttgttg
      841 atcaatacaa aaaagatatc ctgtttatta aataaataat agctaacgaa taataatact
      901 aaagtggcga taatcattaa aatcctaatc atgaacaaat cctccaaaat aagcaacaaa
      961 tagaaaaagc aatttgccaa gagcaaattg ctttattctt taaaagatgg taatctccgt
     1021 taatgccgtt gtttgtcctg tagtattgat cccgcgaatt caagcaggtg ggtcccatgg
     1081 tcgtagcttc gaactaccaa atgataaggc atgttaccag cagtgacaca agacgggtcc
     1141 caagatatca ataaaaatgt tcggtcaaca cacaaagagg acaattcgaa catcacagat
     1201 ttcccacctc tatagtagca tatgaattta aaaagagcaa tgagagagct cgcagaaaca
     1261 gaaccgtttt cttgtctagt tttttggagg atgctttttc cactctttta tacggtaata
     1321 ctgttgaccc agagcctgtt catatttccc tgtatctttt ggctgataat actgagcatt
     1381 cttcagttta tctggtaaat attgctgatt gacccaagcc tgatcaaaat gatgaggata
     1441 ctgataaccc acgccacggt tgagtgattt cgcacctttg taatggctat ctctcaaatg
     1501 atcaggaaca tctccagctt tgccctctcg aatatcagca agtgctgcat ctaatgccat
     1561 gtaggcagaa ttggattttg gcgacaagca aagatcgacg acgacgtctg ctagtgggat
     1621 acgtgcctca ggcaatccca atttttcagc agctaataca gcgttcactg tacgagccgc
     1681 agcagcagga ttacctaaac ctatatcttc ataaccgata accatcagac gccggcaaat
     1741 acttgccaga tcacctgcct caactaaacg ggcgagataa tgaagtgcag catccacatc
     1801 acttccacga atcgattttt gaaaagccga aataacgtca taatgagcat ctccattttt
     1861 gtcatgggta agagcttttc tttggacaca ttcttcaatg atcgacagtg taagatgaat
     1921 ccttccttct ttatctgaag gagtcgatag tgtagccagt tccagcccat tcaatgcact
     1981 ccgcaaatcg ccgttcgttg cacgcgaaag gtgaagaaga gcatcttcgt ctaactggat
     2041 tgcttgttgt ccaaggccgc gttctttatc ccttaaagca tgttcgactg ccaactggat
     2101 gtcttgttct gttaatggtt tgacttcaaa gatctgtgta cggctgcgga tcgctggatt
     2161 gatggtaata taaggattct cagtagtcgc tccgatcaag atgattcggc cactttctaa
     2221 atgaggaagc agaaaatcct gttttgtttt atctaatcgg tgtacttcat ccaataagag
     2281 gatcactgta ccactcattt ttgcttcttc cgctacaact tgtagatcct tttttgaatc
     2341 tgtagcggcg tttagcatgc gaaatgcata attagtagag ccggcgatcg cacttgcgat
     2401 actcgttttt ccagtaccag gaggtccata tagaatcatg gaagagagca tgcgagcatc
     2461 gaccatgcgg cgtatgattt ttccttcccc gactagatgc tgctgaccaa cgacttcatc
     2521 gatcgttcga ggtctcatgc gataagccaa tggctgttgc ataggatttt cctccttctt
     2581 gtttttacgt cccttaatta tatcatattt agcaagatgc ctagaaagaa cagccttctg
     2641 ttgttttgtt ttcatagctt atccaactaa tattgtgtat gataaagtag gtgagaaaaa
     2701 tgacggataa acaaaagata gatgaatttt tttggaataa aacgacgcca gaagtggcac
     2761 gggatttatt ggggatgtat ttggagcatg acacaccaga aggaagatta gctggatata
     2821 ttgtagacgc ggaagcatac ctaggtccag aagacgaggc cgcccatagc tatggtttaa
     2881 gaagaacacc gcgtgtgcgg gcaatgtatg aaaaaccagg tacgatctac ctttacacga
     2941 tgcatactca tcggattttg aacatcatta ctcagccaga agggattcct caaggagtaa
     3001 tgatacgtgc aatcgaaccc gccgcaatga ttgatcaaat gagtaaaaat cgtggcggaa
     3061 aaactggtcc agatatcagc aacggtccgg gaaaattagt ggaagcatta gcaattcctc
     3121 aagaacttta tggtcagtct atagcagata gttcgttacg cttagttttt gagaaaaaaa
     3181 agacaccaaa aaagatcaat gctttgccta gaatcggtat cccaaacaaa ggtgtatgga
     3241 cagaaaagcc gttgcgtttt gttgtgtcgg gaaatcctta tatctcttta caaagaaaaa
     3301 accaaatcga gaaagattgg ggttggagga aagaaaatga aaaagaagga tctattaaca
     3361 tatttagatg aaataattga aaaacaagtg acagactatg atgtagcttt agattgggat
     3421 acaaaaaatc atacgatcga gatcgttgtt cgcttgtttg cagaaaataa agcacaattt
     3481 gagattgatg atgcagaagg gatcgtttcg caagaaccaa ttatcgaatt tgaagacggc
     3541 gtacttcttt ttaatcctca gaaatctgtt tttgatgaac gcaattatct agcagtaatc
     3601 ccttatgaag gaaaaaaagg gttggcaaaa tcagtagctg attctttagt ggaatatcta
     3661 aatgaagtat tagctcaagg gcaaagcgat ttgcttgatt ttttggatga agacgatgac
     3721 gaagctgtat tcgagcttca ctgggataat cagcaactag cagaattagt agaagtaaaa
     3781 cagcaaaatg aagcagatac ttatttaccg tacccaagtt attaaggagt gacagatatg
     3841 aaatggaatg aagtaaaagt agaaactgaa agtgaagcag tcgaagcagt tgctaatatt
     3901 ttgatggagg caggggcgag tggtgtcgcg attgaagatt ctttagatgt tgaacatttt
     3961 cagtcggatc aatatggtga gattttagat aaaaatgatt ttacatcgct gaaagaaggc
     4021 gcactagtca tggcttattt tccggaaacg actttcttac cggaaatctt gccttttatc
     4081 aaagatcgta tcacacaatt acctgagttt ggtctagcta ttggaaaaaa taaagtaaca
     4141 gttagtgaag tagaggaaag caactgggca acagcctgga agaaatacta tcatccggtg
     4201 cgtattacgc gaatgctgac ggttgcgcca agctgggaaa actaccagac agaagatcct
     4261 ctagaaaaaa ttatcacact agatcctggt atggcatttg gcacaggcac tcatcccacg
     4321 acttccttga cattgacagc actagaaaca gtcttgcgtg gtggtgaaac attgctagat
     4381 gtgggaactg ggtcgggcgt gctaagtatt gcagcaaaat atcttggtgc aaaagatgtc
     4441 tatgcctttg atttagacga agtagccgtt cgttcggcga aagaaaatat ggatatgaat
     4501 gaaatagcca aagatgtcca tgtatcagct aacgatcttt tgacaggaat cgagattgaa
     4561 agtgatgtaa tcgttgcgaa cattttagca gatatcatct tattgatgat cccagatgct
     4621 tggcgtttac tgaagcaaac aggcacgctg atcgtttcag gaatcattga agagaagaag
     4681 caaatggttc tggatgccat gttagaacaa gggtttatcg tggatcaaat tttccagcaa
     4741 aaagactggt atgcaattat tttgaaaaaa ccagaggtgg aatagatgca gcggtatttt
     4801 ttaaaagaat catatgaatc aaaagactat tttgttttag aaggggaaaa ctatcaccac
     4861 attgtacggg ttatgaggat ggaacccgag gatgaagttt ttttggcatt caatgatcaa
     4921 atagccattg ttgcagaaat cacggagatt actgaagagg ctgtttatct gaaagaaatt
     4981 aaaaaagaag aaaatcaaaa ggaactgcca agttcgatta ccatagcgag tggctatcct
     5041 aaaggggaaa agctggattg gatcgttcag aaatccactg aattaggagc gcaagctttt
     5101 cttggtttcc cggcatcagc ctctgtcgtt aaatgggatg caaaaaaacg tatgaaacga
     5161 caacaacggc ttgaaaagat tgctcaagag gcagcggaac aatcccatcg gcagatcgtg
     5221 ccatcagtag agttattgga aacgaaacaa gatttatttg agcggctaga tgattatgac
     5281 gtggtcttgg ttgcttatga ggaatcagca aagcaaggcg aaaaatcaaa tgcagccaaa
     5341 attcttagtg aaacaaaacc agatagtcga atcttagctg tctttggacc agaaggtgga
     5401 ttatcccgcg atgaagtcga ttattttgta gaacacaaag ggatactttg tggattagga
     5461 ccaagaatcc ttcgtacaga aacagcacct ctttatttat taagtgctgc tagctatcaa
     5521 tgggaattaa tgaatccttc tttcagctaa cgttcttcat taataaaaga gtatcgacag
     5581 aaagaattga aaaaatagtc aacaatttgt ataatagaaa caattattta tacaatggat
     5641 acgaaaaatc gtttgaaaaa gcttttaaaa agcgaacatt tccgtaaaaa aacgtataac
     5701 tgcacggatt ttgggacaat tatcaaacag atatttgtcc cgattcccgt tttaccgaaa
     5761 aaagaaaggg ggatcgattc ctgtgacaaa agaagaaatt ttaacgggac ccggtgtcat
     5821 aaagctcgtt agtcaataca tgggtcccga acatgtagca tttgttgaga aagcatgcga
     5881 atacgcgact gctgcacatg acggacagtt tagaaaatca ggcgaaccct acattattca
     5941 tcctatccaa gtcgcaggta tattagcaga tttaaaaatg gatccccata cagtggctac
     6001 aggcttctta catgatgttg ttgaagatac agaaatcact ttagaagatc tgagagaaga
     6061 atttggcgat gacgttgcta tgttagtgga cggcgtaacc aaattaggga aaataaaata
     6121 taaatcccac gaagagcagc tagcagaaaa ccaccgaaag atgctgctag caatggctca
     6181 agatttacga gttatcatgg tcaaattagc tgatagacta cataacatgc gtacgttgaa
     6241 gcacctgcga gaagataagc aaagaaggat cgctcaggaa actttagaaa tctatgcgcc
     6301 tcttgcacat cgtctaggga tcagccggat caaatgggaa ttagaagata cagcacttcg
     6361 ttatctaaat ccaaaacagt attaccgtat cgtccacttg atgcagacga agagagaaga
     6421 acgtgaaaaa tacgtaagtg gtactgttga agatattcga atagcgacag aagagctggg
     6481 gatttttgca gaaatctatg gacggccaaa acacatttat tcaatctatc gtaaaatgaa
     6541 agatcagaaa aagcagttca acgaaattta tgacttgcta gctattcgag tgatcgtaga
     6601 ttcaatcaag gattgttatg ctgtattagg agcaatccac acaaaatgga aaccaatgcc
     6661 cggcagattt aaggattata ttgccatgcc aaaagctaat atgtaccaat ctttgcatac
     6721 taccgtgatc ggtccggcag gaaatccggt agaaattcaa atccgaacac aggaaatgca
     6781 tgaaatcgct gaattcgggg ttgctgcaca ctgggcctat aaagaaggaa aaaatgaaaa
     6841 agtagaacca gatggtatga cgaaacaatt aagctggttc catgagatac tcgaacttca
     6901 agacgaaagc tatgatgctt ctgaatttat ggaaggcgta aaaggagata tctttagtga
     6961 taaagtctac gtcttcacac caaaaggaga cgttactgag ttaccaaaag gatccggacc
     7021 attggacttt gcatacagta tccatacaga tatcggtaac aaaaccactg gtgcaaaagt
     7081 aaatggcaaa atggtgcagc ttgattacaa attgaaaaac ggagatatca ttgagatcat
     7141 gacttctcca aattcatttg gcccaagtcg cgactggttg aaattagttg ctactagcaa
     7201 agcaagaaat aagatcaaac gtttcttcaa agcccaagat cgagaagaaa atgtgatcaa
     7261 aggccacgaa tccgtggtca aatgtattac agatctagga tttacgccta aagatatttt
     7321 gacgaagaac aaactgcaag aagcactcga tcgttttaat tatcaaacag aagatgatct
     7381 ctatgcagct gtagggtatg gagaagttag ccccttgacg atggccaatc gtctgactga
     7441 aaaagaacgt aaagaacaaa aaatcgagca gcaaaagcaa gaagcagaag aaatcatgaa
     7501 tcagccgaaa aaagaacctg acaaaatgaa agtacgtcat gaaggtggcg ttgtcattca
     7561 aggggtagaa aacttactaa ttcgtatcag tcgctgctgt aatccgattc ctggtgatga
     7621 tatcgttggt tatatcacta aaggcagagg gatatccatt catcgtcgag attgtccgaa
     7681 tgttcagcct gacaaaccaa atgtagcaga acgtttgatt gaagtcgaat gggaagatac
     7741 atcgaataca cgaaaagagt atgatgcaga tttggaaatt tacggctata atcgttcagg
     7801 cttattgaat gatgtacttc aaacagtcaa tgcgctaacg aaaaatctca acagcgttga
     7861 agcacggacg aataaagata aaatggcgac gatccatttg acggttggta tccagaattt
     7921 atcccaccta aagagtatcg tggataaaat caaagcagta cctgatgtct acagtgtacg
     7981 ccggacgaat ggataggagg acagatatga gagcagttgt tcaacgggta agtaaagctg
     8041 ctgtgtcagt cgatcagcaa gaaattggaa aaattggcta cggcctactt gttttattag
     8101 gcattcatga aaccgatacg caaaaagatg tggactatct ggtaaaaaaa attgcacaaa
     8161 tgcgtatttt tgaagatgaa caaggaaaaa tgaatattag tgtagaagat gtgggaggac
     8221 aaattttaag tatctctcaa ttcacactgt ttgcggatac gaaaaaagga aatcgaccaa
     8281 gctttgtgga agccgcacga ccagaaacag ctattccttt atacgaatca ttcaatgaag
     8341 gaatcagaag tcgcggcatc acagtcgcta ccggacaatt cggagcagat atgtcggttt
     8401 ctctcgtcaa tgatggacct gtaacgatca ttattgattc taaaaacaaa taaagtaatc
     8461 tggttttcac tagtaaaaac tatgaaacac gtcgcttaag ttagactgat aaactctaac
     8521 ttaagtggct tttttgtctg caaaaagcgc ttcttttgtc agcagtcgga aaaaaagtta
     8581 agcttaaatt atacaagtta ttttttatag gaggttgctg aagatgaaag aagtaccagt
     8641 agtagatatc aaaatcacag atttaaaaaa attgtatcaa aaaggataca acatgttgga
     8701 ggagttacgc catgaagcgg acgctcccgt cgtcaaagca aagattttca ataaagaagc
     8761 tatcacgatc tacggatcat cagcagcaaa agtattttat gatccaagga atttcaaacg
     8821 aaaaggtgct atgccaaaat tagtattgaa gacacttttt ggtcaaggtg gagtacagac
     8881 tttggatgga gctgctcatc accatcggaa aaatattttt atggatctaa tgacaccgga
     8941 aagaatggaa gattatcatc gtatcttaga taagaatctg acacaagcac ttgaagcaca
     9001 gcatggtcaa tttgaactat ttgatttaag taagatggtt ttctttacat ctatttgcga
     9061 gtgggcaggt atcaatttat cagcgatttc taaagatgag gtcgaaaaat tagcagaata
     9121 tcaaatctct atgatcagcg gaacgtttac ttctccgatt gatcacataa aaggtgtcga
     9181 aaatcgcaaa aaatcagaaa aatgggcgca aggcttgatt gaagaagctc gacaaaatcc
     9241 tgtggctgga aaagaaaatg tggcattata tgcttttgcc aatgccacag atttagatgg
     9301 tcagctttta cctctggaag tggcagctgt tgaattattg aacatcatcc gtccaacggt
     9361 tgctctaact gtgtgggcgg cattgatggg acatgcactt ttcagtagac cagatttgta
     9421 tcagcaatta aaaaatgact tttcgacgtt acaagatccg tttattcaag aaatgagacg
     9481 ctattatcct ttcttcccaa tgctacctgc gatttcgctg aaagaagtag aagtagatgg
     9541 ttatcgtata ccagaaggca gttgggttat tttagattta tatggaacag accatgatga
     9601 acgaacagtt gaagcgccag attcctttat gatcaaacgg tatgtaggga aagcaaaaga
     9661 tatttcctat aaagaagaat atgagatgat tgcacaaggc gggggaaatt ttcgacaaat
     9721 gcatcgctgt gcaggagaat ggatcactct tcatagtcta cgtgtgtttt ccgatcagct
     9781 agtaaataag tttgagttct ctgtacctga acaagattgg acgattccat tcaatcagtt
     9841 tccgacttat ccgaatagcc gagcgctact ttacaagaat tagattagca taaacttatc
     9901 gaaaaaaagg aagatcaaac agtgctgatc ttcctttttt catattacta taaattgata
     9961 aggtcaattg atcaaaatcc acgaaatgct tcatcaatgg tttgataatc ttcattggat
    10021 aaatgcaaat ctaaagcttg gacattgtca gatacttgct ctggacgctt agctccaggt
    10081 ataactacag acactcgtgg attcttcatg taccaagcta aaacaagttg cgcaggagtg
    10141 gcttggtatc ttttagcaat ggggcgaagt acatcgacag cggttaaaat ttctctgaaa
    10201 cgttctcctt ggaaatctgg atttcttttt cgaggatctc cttcaccaaa ttgtttttcc
    10261 tcaccaagtt catattttcc tgtcagcaaa ccggaagcaa gaggaaaata gggaacaaaa
    10321 ctgattttgt ttttttcaag ataagggaat aattcttttt cagcctgccg atggatcaaa
    10381 ctgtatttat cttctactac atcgacatat ccatctgcat tcgcttcttt caattgttct
    10441 aacgtgaagt ttgacacacc gactgctcgg atttttcctg cttcttttaa ttcgtgcaat
    10501 gtagcaactg attcattttt tggtgtgctt tcatccggaa agtggatata aaaaatgtcg
    10561 atgtaatcag tttgcagacg ttttagtgca tcttccaccg attgtttgag aaactcagga
    10621 gaattatcaa atgttcgccc tttatttggt acatgagcag cttttgtagc gatgacgaca
    10681 cgtgagcgat catattcttt ttccttcagt acctcgccaa tcagttcttc ggaacggccg
    10741 tttccgtaag cataagcggt atcgatcaaa ttgatcccgc tattcaaagc tgtacgaact
    10801 acttgttttc ctgtctcatc ttccaaaccg tcaaaaagat tatgtcctcc aacggcattt
    10861 gctcctaatc caagctgttc tgcgtagact tgtgaatgtc cgattcttat ttctgccata
    10921 gctgattcct cctttttgtc tagtctaaaa caaaatacca aaaagctgaa ctaaaacgtt
    10981 tttgtgaggg gaaatagtca gatatctctt gactttgaga ctggttttta gtaggttata
    11041 agtaatatcg aatgtcaatg aaagagcgag taagtttttg aaactttaca agagaggatc
    11101 atcgaggctg aaaatgatct aaagtttcat tagcctaaag acactctgga gatggacgag
    11161 tgaaaagccg tcccgcgtac gagcgttatt cgtaagagga aagagaaatc tttcaaaatt
    11221 aggtggtacc gtgtatgaat ttcattcacc cttgttcatg atgaacaagg gtgatttttt
    11281 tatttacata gctaagaaag ggagagaaaa ctatgagttt ccaaagacca aaaggaacaa
    11341 atgatatttt gccaggcgaa tcagaaaaat ggcagtttgt cgaagaaaca gcacgtttat
    11401 tattcagaga ttatcagtat aatgaaatgc gtacaccgat ttttgaacac tatgaagtga
    11461 tatcccgaag tgtaggggat acaacggata tcgtttcaaa agagatgtat gatttttatg
    11521 acaaagggga acgccatgtg acgcttcgac cagaaggtac agcaccaatc gttcgttctt
    11581 ttgttgagca taaattgttc ggcccagagt atgcgaaacc gttcaaaact ttttatatgg
    11641 gaccaatgtt tcgctacgaa cgccctcaaa aaggacggtt acgtcaattc catcaaatcg
    11701 gagtagaagc atttggatca gaaaaccctg caactgatgt ggagagcatg gtgatggcac
    11761 ttgatttctt caaacagttg gggatcacgc aaatccggct agtcatcaat tcattaggtg
    11821 ataaagaaac gcgtcaagcc tatcgtcaag cattgatcga ctatctttta ccttttgaat
    11881 cagagctgag tgaagactct aaacgccgcc ttcatgaaaa tccattacgc gtattggaca
    11941 gtaaagacaa acgagatcaa aaatttgtgg cagaagctcc atctatctta gattatctaa
    12001 gcgagcctgc tcaaaaacat tttgataccg ttgtttccat gctggatgca ttggctgttc
    12061 cttatgaaat cgacagtaat atggttcgcg gactagatta ttacacacat acgatttttg
    12121 aaatcatgag tgacgcacca aaaatggggg cacaagcaac gatttgcgca ggcggacgtt
    12181 atgacaatct agtggaagaa ctaggtggtc cttcaacacc tggcttcgga tttgcaatgg
    12241 gtatcgagcg attactcttg actatggagg cagaggaagt agttatccct gcatttaatg
    12301 agttggatgc ttacgtggtc gcactcggag acgaaacaaa tatcgaagca ttgaaagtgg
    12361 tccaagcgat tcgaaacttt ggtttttcag ctgaccgcga cttcatgaac cgtaaagcaa
    12421 aagcgcaatt taagacagca gataaagcga atgccaaatt agtattggtg atcggtgaag
    12481 atgagcttgc tagtcaaacg attaatgtca aagcaatggc aagccgaaaa gaaaaaagtt
    12541 ttccattaga agagatatat gaacattttg atgaagtcta tgatgaaatg actttattcg
    12601 agtaggagga aaaaaataaa tggcacaaag aacgatctat tgcggattag tttccgaaca
    12661 acaattagaa caaacagtta cattaaaagg atgggtacaa aaaagacgag accttggggg
    12721 agtaatcttt atcgacctac gtgatcgtga aggaatcgtt caaatcgtct ttaatcctga
    12781 aatcaacaaa gaagcatggg aaatcgcgga taaatgtcgc agtgaatacg tgatcgaagt
    12841 gactggggaa gttagaagac gcggagaaaa tgcagtaaat cctaaaatgg ccactggtga
    12901 attcgaagtg attgcaagtg aaatcgtgat tttgaatcaa gcaaaaaccc cgccatttca
    12961 aattgaagat gatcaagcaa tcagtgacga aatacgtttg aaatatcgtt atttggatct
    13021 gcgtcgtcca aaaatgacta aaaacatcaa attaagaaat gcgacaacaa aagcgattcg
    13081 tcattattta gatgataatg actttctcga tatcgaaaca ccttacctag ggaaatctac
    13141 accagaaggc gcaagagact acctagttcc ttcacgtgtc catgctggac atttctatgc
    13201 gttgccgcaa tctcctcaat tgtttaaaca attgctgatg aatgctggat tcgaccgtta
    13261 ttatcaaatc gttcgttgtt tccgcgatga agatttacgt ggagaccgtc agcctgaatt
    13321 tacgcaagta gatattgaaa caacgttctt aacagcagaa gaaatccaaa cttacacaga
    13381 gggattgatt gcgaaggtaa tgaaagaagt tcgcggtatc gaagtcacat tgccatttcc
    13441 tcgtatgacc tatgatgaag caatggctcg ctacggcagc gacaaaccgg atacacgttt
    13501 tgatatggaa ttgatcgatc taagcgatac agtcaaagaa gtagaattca aagtcttcca
    13561 aatggctctt gaaaacggcg gtgtcgtgaa agcattgaat gctaaaggcg ctgcagatcg
    13621 ctactcacga aaagacatgg atcagttagg tcaatatgta ggacaattcg gagctaaggg
    13681 gcttgcatgg ctgaaagtag aagaagacgg attgaaaggt ccaattgcta aattcatggg
    13741 agaagcaaca gaagcaatca ttaaagcaac agatgcaaaa ccaggtgact tgctgatgtt
    13801 tggcgcagat aaaagtgaaa tcgtcgctgc tgcattaggt gctatccgga cacgtttagg
    13861 aaaagaatta ggactaattg atgaatcgaa attcaatttc ttatgggtaa ctgattggcc
    13921 acaatttgaa ttttcagaag aagaaggtcg atatgtctct gctcaccatc cattcacgat
    13981 gccaaaagaa gaagatatcc cgttattggc tgaagaccct gcaaaagtct atgcagaagc
    14041 ttatgatatc gtcttgaacg gttatgaact aggcggaggt tctatccgta ttcatactcg
    14101 tgagcttcaa gaaaaagtac tagaaactct aggctttaca aaagaacaaa tggaagaaca
    14161 atttggtttc ttgttaacag ctttagatta cggattccct ccacatggcg gtattgcatt
    14221 aggattggac cgttttgcaa tgttattagc cggagaagat aatattcgtg aagtcattgc
    14281 ctttcctaaa aacggaaaag cagcagatgt gatgagtgat gcaccaagta tggtttcgcc
    14341 tttacaattg ttcgaattga atattgatgt aacgaatata gaagaatagc cgataaaaag
    14401 gtaaaaagga tttgtcgaat acgcttcttt tgacaacctt gatttaattg aaaaacactt
    14461 tataatgaag ccataacata taaagcgggg cttttccatg aaaaaaacat tcgaaaattg
    14521 ggcttatcat gattacacga ccaagctttc ggtatctatc gtttatgcgt tgatcgcatc
    14581 agtcgcattg aatttctttt atcagccagg acatatttat tcaagtggta ttactggtct
    14641 agctcagatt ttgacgactt tgtcaaaatc actgatagga ttcgaagtac cggtatcaac
    14701 aactttgtat ttactgaata tacctttgtt tgtattggcg tggttaaaaa tcgggaagaa
    14761 gtttacaatt ttcactttta tcacagtcac tttgacctcc ctttgtatcc atattttacc
    14821 ggaaacagtc ctatctaccg atccaatcat ctgtgcgttg tttggggggg ctgtgatggg
    14881 actaggtatt gggctagcgt tgagaaacgg cctatcatcg ggcggtctag atatcgtgag
    14941 tatcacgatt cgtaaaaaaa caggaaagtc gattggttct atttcgatct acttcaatgc
    15001 attgatcgtt tttgttgccg gaattctatt tggctggcaa tatatgtttt acagtacgtt
    15061 gtctattttc gtcagtggaa aagtaactga tgtcgtttat actaaacaaa agaagatgca
    15121 ggtaatgatc gtaacgaaac atccagaaaa agtagtaaat gaaatccaaa actgtatgcg
    15181 tagagggatc acgatcatca atgaagcaga aggtgcttat aagcatgata agcagacagt
    15241 attgatgacg atcgttacac gatacgaact gcctctactt agagaagcaa tgaaagcttc
    15301 tgatccacaa gcttttgtaa gtattgcaga aaacgtccag atacttggtc gtttctatga
    15361 ggatggcatt tagatcgagt ttgacaaaaa ttcatgaaat actgctgtag acagacgttc
    15421 gtttgtctgc agtttttttc taccatagta gaatcagcaa agaaaaactg cttttttatt
    15481 tgaaattttc ttaaagttga aaaaaagcat gtttattttt ttaaatttat gggatgatag
    15541 aaaaaaatag tagagaaagg acacgattct acttgctatt ttcacttaat acccgtatat
    15601 tacgaatgaa aaaagtttat gaaaagttgg aatattgaat ggaaaaacaa aaaaggttaa
    15661 aaaacagccg ctacatcact gggtttgatg ggctccgttc attagctgtg gtgggtgtta
    15721 tcttctatca ccttctgcca actagtatga aaggcggcta tttgggcgtt ccgatttttt
    15781 tcgtcgtatc tggttatttg atcacggatc tgctgcggca ggaatgggaa caaaatggaa
    15841 aaatcaatat ttggcaattt tatgtcagaa gaatgaagag actctatccg gcacttgttt
    15901 ttatgcttgt gacagcttcg gcttatatca ctttgtttca gcgagggctg ttaaacaatc
    15961 ttcgaggagt tgtggtcagc agcttactgt atgtcaacaa ctggtggcaa atcaataacg
    16021 gtttatctta tttcgatcga tttgctaatg agtcaccgtt tacgcatatc tggtctttag
    16081 cagtagaagg tcaaaactat ttggtttggc cgatcttgtt tgtattgttg atggtatttg
    16141 ttcgtaaaag aaaatggatc ttttatactg tgttaggtac atccttcctc tccgccatct
    16201 tgatgatgat cttgtataca ccaggaggcg atccaacccg tgtttattac ggtaccgaca
    16261 ctcgtctgtt ttctatatgg ttgggaagtg cgcttgcgtt tgtttggcca agtacgcgtt
    16321 taaagaaaaa catcccgaaa caagcgaaaa atattctgaa tatcgctgga ggaatctcgc
    16381 ttttggcact gattctttgc ttcttctttt tagatgatca ttacaatttc atctattacg
    16441 gaggaatgct cctagtgagt ctgttatgcg tagtgctagt tgcagtgaca gcacaccccg
    16501 gagcaagttt gaatcgatgg ctgacaaatc cagtatttac ttggattggc aaacgaagct
    16561 atggcattta tctgtatcag tttccagtta tgatttttta tgaagcaaag gtgactgata
    16621 ttgccgatca tgttttgttg catactttag tggaaatcac attgattcta atcatcagtg
    16681 aactttctta tcgatatgtg gagcgtccgc tagcacgttt tgattatggt cgtacattcc
    16741 aagtgatgaa aggatggttc agcaaaccga tcatcagtaa gagcaaacca tgggttgttc
    16801 caggcacatt acttgtagca attgcactcg ttggtttcgt tacggcaccg aaaaacgctg
    16861 taacagccga tcaaaaacaa ctgcaggcaa aaatcgctga aagtaaaaaa ttagcagaag
    16921 aaaccagaaa caattcagaa aacaccacta gttcggttga ccaagcgatc cttgataagt
    16981 acgagttgtc caaggcacaa gggaataagg cacaaaaatt agaactaacg gcatttggtg
    17041 attcagttat gttggatgct gcaacggatc tacaagaact ttatccaaaa gtagtggtag
    17101 atggagatgt cggacgacaa ttatatgcca gtgtaccgta cattgaaaaa ttaaaggaac
    17161 aagatttgtt gaaagacact gttctgatcg gactaggaac gaatggctca ttctcggaag
    17221 ctcagtttga tagtgtgatg gatgcaatcg gtgatcgaaa agtatactgg gtcaatgtcc
    17281 gagtaccgac gaagcgctgg caaaatgacg tgaatgcgat gcttgaacag atgacgaaaa
    17341 aatacgacaa tatgacgctg attgattggt acagctacag taatgaccat agtgactggt
    17401 tttatgatga tcaagtccat ccaaatcctg aagggatgac acattacatt cacttagtca
    17461 gcgaagcctt gctaggagga agttcaagta cgaacagcag tgagacaaac gtgacagaat
    17521 gatagctaca aaaaacttca ggcaaaaaca tttgtttttg cctgaagttt ttttatattt
    17581 accgtgtttt taacattcat atagattcag gaagcaaaac ggttgagtgt gtctgtaaat
    17641 aacgttcttt ttgttttcgg gtgagttttt tgaatgcagc ttctgctttt gttgcttcgc
    17701 ttcttgaaga aaatacttct gcatggatca tcttaacagg tcttcttttt tgaagacgag
    17761 tatatttagc accagtacca ttgttatgtt cctctaatcg tctagaaaga tcggttgtat
    17821 atccaccata aaatgtctga tctttacaga ataatacgta aaaataatgt tcatttgcca
    17881 tagagcatct cacgaatctc tgcagtgtat tctccatcca tattgtaagt gatcaatggt
    17941 ggagcgactt tgaatccgtc taatttgcct tctttgattc cttcaactaa caaaatattt
    18001 gcctcttttc caggttttgg gtaaacaaat tggatccttt taggtgcaat tcgatagtct
    18061 tccatgacct gaatgatttc taagaaccga tcaggacgat ggaccatagt aaagcgtcca
    18121 tttgttttta gtagcttact tgaaatatcg atcacttctt ctaacgtcgt atgaatctca
    18181 tgacgggcga ttgccaggtg tttattaggg ttcgttttat ttgttggcag ccctttgaaa
    18241 taaggtggat tacagacgac taagtcacaa gaattatgtc gaacgacaga taagctgtct
    18301 ttcagatcga tggtgtggac agtcatttgt tcatttaaat ggttaagcgc gatactacgt
    18361 cttgccatgt ctgctagacg aggttgaagt tcgatagcgt cgatagccgc ctgggttcgt
    18421 ttacttaaaa aaagtccaac tgcgccattt cctgcacaaa ggtcaacaat cttccctttt
    18481 ttaggtacac gggaaaagtg agcaagcagc acagcatcta aagaaaaaga aaacactttg
    18541 gaactttgga taatcttgat atcttctgcg tataattggt cgattcgttc gtcttcgtga
    18601 agcatggatt ccctccaaaa tgaaaaaaat agtcgtctgt attatactat ttttattaaa
    18661 aagaaccaag gtggcttgtc aaacctctat tttttatgca tactatgacc ataagaaggg
    18721 aaaggaagag actatgtttt atcgatttat gcgcggggta gttcgagtac tattagcctt
    18781 agttaatgga aatgcccgct atgagaataa agaagtttta ccgcaagatg agaattacat
    18841 actagttgct ccccatcgta cttggtggga cccattatat ctggcggtag ctgcagcacc
    18901 caaggaattc agctttatgg caaaagaaga actgttcaaa aatccagttc tgcgatttat
    18961 attaactcga tccaatgctt ttccagttaa aagagaaaat ccaggaccaa gcgtcattaa
    19021 aacacctgta aagattttga aatcgacaaa tttgagtttg atcatgtttc ccagtgggac
    19081 aagacatgct acagaactta aaggcggaat ggcattgatt tccagaatgt ctaaagcgaa
    19141 aatcgtgcca gctgtatatc aaggtccgtt gacacttggg gatctgttca agcgtcagag
    19201 agtgacagtg cgtttcggcg aaccaatcga cttatcagat atcaagaaaa cggataaaga
    19261 aggtatggcg gagatcgaac gacgaacaca agcagctttt gacaaattgg acaaagaagt
    19321 gaaccctgat ttcaaatatg aagtcaaaaa aaaataaaat gataagttta tgcgtgggcg
    19381 gtcttttgaa tctactgtcc tgttcagttc agactttttc aatgaaaacg agggagagac
    19441 gtcaagtctg ccctcttttt gcgttccaat atgtaaaata gaagacaggt gtcatgtaat
    19501 ggatcaaagt gttataataa ttagaaggac attttttgag gtgattgaat gcgtttttta
    19561 catacagccg attggcatat cggaaaaaaa ctacaaggtt atgacttgat tgaagaccag
    19621 cgtcatgtac tatcttctat actagatatc gcgaaagaag aaaaagtaga ggcaatcgtg
    19681 attgccggag acttatatga taggagtgtc ccagctgttg atgccgtaga attatacaat
    19741 gaattgatgg tcgattggaa tttaaaggaa aagatcccga tttttgctat atcaggaaat
    19801 cacgatagca gtacgagact ttcagcgggt acgccatggt tcagttattc gaactatttt
    19861 ttgcgaacac agctagcaga agcatttgaa ccagtcgagt ttaaaaacgt acaatttttc
    19921 tttcttcctt actttgaacc aattgcagcg agattatatt ttgaagatga caatatccga
    19981 acgatcaagc aagcggtagc caaggtgata gataaaatgc aggagctatt tgattcagaa
    20041 aaaaaacaag tattgattag ccattttttt gtaatgggaa gtgtaaaatc tgattcggaa
    20101 acgaaaatcg aagtaggcgg gttggattcg atccctggtg aactacttac accgtttgat
    20161 tatgtagcac taggacattt gcataatcaa gctgctttga gacaggataa tgctcgctat
    20221 agcggttccc cattgaaatt ctctttatct gagatcaacc aaaaaaaagg cgtatggatc
    20281 gttgacctga caacaggcct ggagttgaat tttagggaga tcgaacccct gcatgatatt
    20341 gaagaaataa cagcgagttt caaagaattg ctggatcctg ccttttatca gtccgtcaat
    20401 cgggagaatt acatctatat ctatctcact gatagaacga tcatcccgaa tatgatgaac
    20461 cagcttcggc aaatatatcc tcgtattttg gggattgaac gggcaaacgg tcgagcagaa
    20521 caaacaaagc gcaaacgaaa agtactagaa atgaaagatc ctcaaaaatt agcagaagca
    20581 ttttttagtg aaatgacgga agagcagcta actgcaaacc aagaaaaatg gctcggagaa
    20641 acgttgcaag aaatcatgga aatggaatga tcaagaatga aaccgaaaaa attacaactt
    20701 aaaaattttg gtccttttat caatgagacc gtagattttt cacgtttgac agaagcaccg
    20761 ctttttttga tcagcgggaa gacaggagct ggcaaaacga cgatttttga tgggatcacc
    20821 ttcgcacttt ttggtgaaac ttccggaaga cttcgctctg gaaaagagat gcgttctttg
    20881 tttgctacac cagacgaaga aacaagcgtg actttttctt ttgaacatca gcagatgagc
    20941 tatgaaatcg aacgaaaacc agaacaagtt ttagcaaaac gaaaaggaaa cggaacaaaa
    21001 aaacaggcag ccaaagtaac gttgaccatt tttgatggga aaggaaaaga aataaaacaa
    21061 atcacaaaac gtacagaagc agatcagttg atcagagaac tgatgaatct cgatgcgaaa
    21121 cagttttctc aaatcgtttt actaccccaa ggagaattcc gtaatttttt ggtttcatca
    21181 agcagtgata aagagactgt tttacgtcat ttattcggta ctcaattttt ccaacagttc
    21241 aatgagcgat taaaagaaaa agcaaaacaa cagcaacaaa aattggatca tttagagcaa
    21301 gagctgcatc ttctgcaaaa aaggtttgtt ttagtcaaag aagaggaacc agcagcaggg
    21361 tttgaagccg ttttagccca atggacaaac catcaggcaa tactcgaaaa acaaatcacg
    21421 gaagaaaaag aagctctaac ggaacttcag gaaaaacaaa agcaactaga aaattcctac
    21481 tatcgttttg aaagcttgaa aaaaagttat gaagaaaaaa cgatattaat aaacaagcaa
    21541 aaggaattag ctgctcaaaa agacgaaata gaggaaaaga aacgatggat tgggtattat
    21601 gaatttacgc aacgattagt cgaaccaatc cagcgcctac aagaaataaa agttgaaaaa
    21661 atagaactgt cacaaaaaag tagtgagcaa acaaagaaac tagcgatgac tacgcagaat
    21721 tatgaagagt ggcaaaaaaa tgaagcaaac cgcgtacgcc gacaaacaaa aatcaaagaa
    21781 gctgctcaaa aactgcaaaa caaacagatg atggtgccaa ttgtcgaatc cttacgtagt
    21841 aaaaaacaag acgcagaaca attaacaatt tcattggata aagaagaatt gaatgccgag
    21901 aagctgtatc aaaatctcgc tatctatcag gaaagaactc gaattcttca agaaaattta
    21961 ctggatcagg aaaaactgca agaagatcgt cttgcttttg gacagttaaa atatctagat
    22021 gaaaagcagc aagaaataga aaaacaaaaa gcaaaagaca ctgggtcgct cgcacagata
    22081 agagaagaac tcaaaaaagc aactgaagaa ataacaaaaa atagtcagct attgaaagaa
    22141 cagacagaac aagcagaaca gctgaaaagc caatgggcaa agcagcaaat tgctcgtctt
    22201 cagctttttt tgcgtccagg ggagccatgt cttgtttgtg gatcaacgga acaccccata
    22261 caacaattaa aacacgaaca gccaaataag gaagatatct taaaagtaga acaacaactg
    22321 gcagaagctg agtctgctgt agaaagaact cagcaaatca ttgccaaaag tgaagcacat
    22381 gaaaataatc ttcaacaaca attatcagat ttagaacaaa aaatcatggc aacgaatata
    22441 caattggctg aacaagaaga acacttgcaa caggctttga aaaaggcatt tcctgagata
    22501 acagaacaaa aaacaaaaac aactcttgta gtaatcgaac aatctttaga tagacgaaaa
    22561 agcaagatgg ccaatgcaag aatagagctg gaagaaataa gacaacaagc agaaaagctc
    22621 gaacaacaat tagctgtcta caaacagcag cagacagaaa aaaataatca gctccagcaa
    22681 ctgcaaggag aaatcaaaag tttagaacaa caattgcaag atacggatag taactgttta
    22741 gaagaggaga tacgacgact tgaaaaacag ttagaagaag actcgaatca actagctgtt
    22801 gagcagaaag caggagtgca attgttgcaa gatcttcata cgcttcggac acagcttgat
    22861 cttcaacatg aacaaataga aaaaatagat gaaaaaatag aaaaggaaca agaaagaata
    22921 gctaaaaagt tagaaagcca gtcgtatttt tcaacaattg aagaaatctg tgcatttgca
    22981 caacagcaag cacaatataa agaaaaccaa cgtacagtta cttcttatca tgaagagcaa
    23041 ttagtgacag ctgaccggct gcgtcaatta gaaaatatcc agctaactga gacattccca
    23101 gatacagaag agatcagcca acagttagaa gaaataagac gtatgatcga aataaagcaa
    23161 gaaaagatat acggtttaca agaacaaaaa attagtaatc aaaaaatcta cgatgaatgt
    23221 ctttcgattt atcacacaag ccagacgaaa ttagatgagc ttacgcaatt acagcagctt
    23281 tcgcaaacat tcaacggaga aaatcctaag aagactagtt tggaacggta tgtgcttcag
    23341 gtttatcttc aagaagtgtt acaagtagca aatgatcatc tccaacgttt aacgaaaaat
    23401 cgttatcaat ttgagctagc ggataccatt ggcagttatc gagggaaaac cggattagaa
    23461 atcaatatct atgatgatca ggcaggtatg tctcgaagtg cgcataccct gtctggcggt
    23521 gagagtttta ttgcagcact ttctttagca ttagctcttg ccgatgtgat ccagacacaa
    23581 gctggtggtg tagcaatcga tgcattgttt attgatgaag gatttgggtc attagatgaa
    23641 gaagctttgg aaatggcaat ggaagcttta gaaacgatcg aaagtgaagg aagaatgatc
    23701 ggcatcatta gtcatgtaag agaattgaaa gaacgagtat tacaacaaat aagaattgat
    23761 acagaaggta gcggacaaag caaaatacgc tatcaacttg gataaaaagg gagattggta
    23821 actatgaaat ggagtattcc agaaaaagtc attgcacgtg gacgtcaata cttgcaagaa
    23881 ggccgagtgt tatcggttgt ccctgaccaa gaagaaaacg tgtggcatgc agaagtcttg
    23941 ggcagtgaat tgtatatggt tgatttggat ggtacagcaa aagagataga ttactgccaa
    24001 tgtccctatt gggaggaaca taaatattgt aagcatacag ttgctgtgga actttattta
    24061 cggtcgaaaa atctatctcg tatcatgaaa aaagaccagc caatgctaga accggtaaaa
    24121 gcaacaagtg agggagaaat cattgcaaaa ggtttttctc gtctaaaaac acaaaacaat
    24181 cctcaagcgg tgcaaccttt gattattgaa taccaagtag aaacgattga gactaatcag
    24241 tatcacccag agctttctat tttggcactt tacttgcgta tcggttcatt ggcatcaccg
    24301 aaaaaaacat atatcgtaaa aaacatctat gattttttac aagcttacac agaaaaagaa
    24361 acttacactg ttaataaaca gtaccagttt cgtctagata aacgggcgtt tcaaaaagaa
    24421 gatcaaaaaa tactcacgca tcttgcagga agtgctcaga cgcatcaatt attaggtacg
    24481 aacggcttac aagtcaaagg aaaactagat aaaagatatc tgcttctccc agtagaacaa
    24541 gcccgcttcc tgttagagca gatgaatcag actacacgtt tgcatctgca gataaatgaa
    24601 caaaacgatc aaggaataca attttccgat caaaacaaac ctttatcatt caaagtggaa
    24661 aaatttggag aagattattt gctgcaagca ttgaatgatt ttgatttttt ctttatgcat
    24721 tatcagtggg gagttatcca agggacattt tattctctga cgaaatatca acaaacggtt
    24781 tatcaaactt ggaaacaatt gattcgccga ttggagaaac cagaagtagt gttcaagaaa
    24841 aaagaattac cgtctttgtt taaagagatt atccctctgc tgtcagagat cggcgacgta
    24901 gctattgccc ctgcagtaga agaggaagta gcagatatcc ccgtcgaatt tattttcttt
    24961 ttccgaaaag caaaaggaaa aatccaagct cgagtcgatt tcgtctatgg cgaagtcatc
    25021 tattcgactg acccgaagca tgaggtctcg acagaacagg cttttcaggt gttacgtgac
    25081 acagtagaag agaaacgagt acttgatcta ttcagaatgt atcgctacca gaaaaatgaa
    25141 actggttatg aacgtccatt gccaagcggg gaagaactat atgccttttt ccgcacagaa
    25201 ctatctgtct tccgtcagtt tggtgaagtc agacttggaa gaaaattgcg agagttgtat
    25261 ttagatgcgc ataaacatca accgaaaatc gaagtaaccg agaccggttc atggctcgat
    25321 attcgattcg atgtgacagg gatagaggag caagaaatcg atcatgtcct gcaaagcttg
    25381 ctgagaaatg atgcttttta tacattagaa aatggtcagg ttctttcttt tgattcagaa
    25441 gaatttcaac aaacaagcca agtgttgcaa caattacggg aatcgatacg tacagaagaa
    25501 ggaacgatcc atgtacctaa aaatcaagga ttgatcattc aaaatcaatt ggaaaaaagc
    25561 aatgctactt ttagtgaatc gttccaaacg atggtacaag atctcattca tccagaaaga
    25621 taccaggcac agcttcccaa aggattgaat gcaacgatga gagattatca aaagcaaggt
    25681 tttcgatggt tgaagatgtt aggtcattat cagtttggcg gtattttagc cgatgaaatg
    25741 ggacttggga aaacactaca gacaattgcc tttttacttt cggaaaaaga agaacggaaa
    25801 agcttctctg ctttgatcgt agcgcctgcg agcttaattt acaactggca ggcagaggtg
    25861 agaaaattcg ccccttcttt aagtatccaa gtaatcaatg ggaataaaaa agaacgtgaa
    25921 gaattactgg ctaaagacac agatatccga gtgacttctt atgccagctt aagacaggat
    25981 ctagctaatt atcagtcgca aaaaattgat tatttgattc ttgatgaggc acaaatggtc
    26041 aaaaatagca gtacgaaaac tgcacaagca ttgagagaat tagctgtacc tcagcggttt
    26101 gcactgagtg ggacaccaat cgagaataat ttagaagaat tgtggtcatt gtttgcaacg
    26161 attatgccag gatttttccc gactaaaaca aaattcaggg aacttgccgc agaagaaatt
    26221 gcgcaaatga ttcgaccgtt tattttacgg cgagataaac aaactgtgtt gaaagacttg
    26281 ccggaaaaaa cagagatgaa tctatattct gcacttacag aagaacaaaa aactgtttat
    26341 ttagcttact tgcgtcaaat gcgtgaagaa atcacatcaa tggattctga agcattcaag
    26401 aaaaaccgga tcgggatact tgctggtctg acaagactgc gacaaatttg ctgcgatcct
    26461 cgtctgttta ttgaagatta ccaaggcggt tccggcaagc tcgaacaagt aaaagatcta
    26521 ctggtagctg ccaaagagaa caaacgaaga gttttgctgt tttctcaatt tacagggatg
    26581 ctgactatct tgcaagaaga gttagccgag ctagggatct cgacatttta tttaagaggc
    26641 agtacgaaac cacaagatcg gttaagtatg gtagacgcat ttaatgctgg cgaaaaagat
    26701 gtttttctga tttccttaaa ggctggggga actggattga atctcacggg agcagatacg
    26761 gtcatcttgt atgacctttg gtggaatcct gccattgaag aacaagctgc tggacgtgca
    26821 catcggatcg gacaaaaaaa tgtggtagaa gtgtggcgta tgatctctga aggaacgatt
    26881 gaggaacgca tggattcact ccagcaagaa aaacgcgaac tgttccaaaa agtgatccaa
    26941 ggtaatgaag aacagttgac gaaactgact gaagaagaca tccgcatgat tttaagtgta
    27001 ggagaaatcg aggagtaaga gactcgaaaa agcgttcagt aatgggtaag aaagcctttc
    27061 taaagaagcg attgtttatt tgatttaaaa aatagcaagg aggctgggat tccattttat
    27121 cccagcttcc ttgctatcat ttttttattt ctatttttaa aggttataca aagattgtct
    27181 tcattgtctc taacagctga tcaaaagaag aaatctcgat atcgcaaatt ggagtctgtt
    27241 caattttgcg ttctcgatgg ttgaaccaaa gcgcttgcca gttagcactt tttgcaccta
    27301 gaacatcatt gtcgtaatta tcgccaacgt ataaggtctc ttctggaagc atatgaaatt
    27361 ctttctcagc tagctgaaaa atctcttttt ctggtttttg gaaacctgtt gcttgagaaa
    27421 tgagcatatt gcgggaagga atccaattgt tcaattgaag ttgcttcagt ttttttgtct
    27481 gatggtcagt cggcccattt gtgataattc ctaatgggac attcttttcc ttcaagaaat
    27541 cgagggtttt tttgacctcc tcatgcaagc agatattatc aagttcttcc tcgtaaattt
    27601 tttgaaataa gaggccatct tcttccgtaa tatgagggta gtctaaatct tttagtgatt
    27661 ggctgatccg atgtgctcgc atatattcta gtgtccaatc tcctgccatt acttttggga
    27721 agttttcatc actatgatgg cggaaccgga tatatagtgg atgcatatct gcatctgaaa
    27781 caagcggaac gactcttttg accgcgtttc gaaatggctg ctgctgatca tacatcgtat
    27841 catcaacatc aaacacaact gatttaatca aaattttaca cgcctttcgg ttttcattga
    27901 ttttttcaca aatccttgac tattgtaaca gaaaaaattg tctaaaaaag ctgataaata
    27961 aaggtttttc ttactatatt tctatttcct tgacagaata aggtttttat atttcagttt
    28021 ttttcactaa aaatcgtgaa acacgtcaat aagtttgatg aacaaattaa tcattatggt
    28081 agaagtcgat tgatttattt taatgcaatc tctgtggaat tttcgaaaga tttattttt
//
LOCUS       DADVXU010000026        28050 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.028, whole genome shotgun sequence.
ACCESSION   DADVXU010000026 DADVXU010000000
VERSION     DADVXU010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 28050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.028"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..822)
                     /locus_tag="KB953_001086"
     CDS             complement(<1..822)
                     /locus_tag="KB953_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBB6866074.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(795..1475)
                     /locus_tag="KB953_001087"
     CDS             complement(795..1475)
                     /locus_tag="KB953_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBB6866075.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1498..2436)
                     /locus_tag="KB953_001088"
     CDS             complement(1498..2436)
                     /locus_tag="KB953_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HBB6866076.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2681..3544)
                     /locus_tag="KB953_001089"
     CDS             complement(2681..3544)
                     /locus_tag="KB953_001089"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBB6866077.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(3686..6259)
                     /gene="mprF"
                     /locus_tag="KB953_001090"
     CDS             complement(3686..6259)
                     /gene="mprF"
                     /locus_tag="KB953_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HBB6866078.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6400..7206
                     /gene="proC"
                     /locus_tag="KB953_001091"
     CDS             6400..7206
                     /gene="proC"
                     /locus_tag="KB953_001091"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBB6866079.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7285..8841)
                     /gene="rny"
                     /locus_tag="KB953_001092"
     CDS             complement(7285..8841)
                     /gene="rny"
                     /locus_tag="KB953_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HBB6866080.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(9252..10301)
                     /gene="recA"
                     /locus_tag="KB953_001093"
     CDS             complement(9252..10301)
                     /gene="recA"
                     /locus_tag="KB953_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBB6866081.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(10410..11651)
                     /locus_tag="KB953_001094"
     CDS             complement(10410..11651)
                     /locus_tag="KB953_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HBB6866082.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(11891..12469)
                     /gene="pgsA"
                     /locus_tag="KB953_001095"
     CDS             complement(11891..12469)
                     /gene="pgsA"
                     /locus_tag="KB953_001095"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBB6866083.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(12678..13601)
                     /locus_tag="KB953_001096"
     CDS             complement(12678..13601)
                     /locus_tag="KB953_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6866084.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(14340..15626)
                     /locus_tag="KB953_001097"
     CDS             complement(14340..15626)
                     /locus_tag="KB953_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBB6866085.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(15619..16887)
                     /locus_tag="KB953_001098"
     CDS             complement(15619..16887)
                     /locus_tag="KB953_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBB6866086.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            17139..17600
                     /gene="mscL"
                     /locus_tag="KB953_001099"
     CDS             17139..17600
                     /gene="mscL"
                     /locus_tag="KB953_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HBB6866087.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(17659..18105)
                     /locus_tag="KB953_001100"
     CDS             complement(17659..18105)
                     /locus_tag="KB953_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB6866088.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(18198..18443)
                     /locus_tag="KB953_001101"
     CDS             complement(18198..18443)
                     /locus_tag="KB953_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HBB6866089.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(18558..19136)
                     /locus_tag="KB953_001102"
     CDS             complement(18558..19136)
                     /locus_tag="KB953_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBB6866090.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(19213..19743)
                     /locus_tag="KB953_001103"
     CDS             complement(19213..19743)
                     /locus_tag="KB953_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HBB6866091.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(19747..20607)
                     /gene="rlmB"
                     /locus_tag="KB953_001104"
     CDS             complement(19747..20607)
                     /gene="rlmB"
                     /locus_tag="KB953_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBB6866092.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(20600..20995)
                     /locus_tag="KB953_001105"
     CDS             complement(20600..20995)
                     /locus_tag="KB953_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HBB6866093.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(20992..22404)
                     /locus_tag="KB953_001106"
     CDS             complement(20992..22404)
                     /locus_tag="KB953_001106"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBB6866094.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(22401..22937)
                     /gene="cysE"
                     /locus_tag="KB953_001107"
     CDS             complement(22401..22937)
                     /gene="cysE"
                     /locus_tag="KB953_001107"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBB6866095.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(23023..23262)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(23259..24719)
                     /locus_tag="KB953_001108"
     CDS             complement(23259..24719)
                     /locus_tag="KB953_001108"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBB6866096.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(24889..26046)
                     /locus_tag="KB953_001109"
     CDS             complement(24889..26046)
                     /locus_tag="KB953_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HBB6866097.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(26080..27450)
                     /gene="radA"
                     /locus_tag="KB953_001110"
     CDS             complement(26080..27450)
                     /gene="radA"
                     /locus_tag="KB953_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBB6866098.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(27513..28040)
                     /locus_tag="KB953_001111"
     CDS             complement(27513..28040)
                     /locus_tag="KB953_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBB6866099.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT         7695 a         6191 c         4964 g         9200 t
ORIGIN      
        1 gttatttttt cttctgaagg caaccaatat tcgtttgatg atatttacaa tcaggaacaa
       61 agcaataaag actaatgtga ttgttgctcc tggaggtgta tctagataaa acgagctagt
      121 tagtcctgac agcatcccta gaaatcctac acaaacactg atagcaatca cgacattgaa
      181 gcttttgcct aatcgcatac cgattgctgc gggaagaacc atgattgcgg atatcaaaag
      241 tgcaccggca atcgggatca tcacagcaat agccacacca gtaatcacat tgaacatcat
      301 cgacatcaaa tgaacgggta gcccatccac gtgggcggta tcttcatcaa aagtcagaac
      361 atacatcggc cgtttgaata aaaagaacaa tataccgatg gcaacgaata agattcctaa
      421 aaagatgact tgctcccatg taatggtcac gatcgaacca aacagatagg attggatact
      481 tgttgctgaa ttccctccgc tcaaattcat caatactaac gctagcgcca aaccaccagc
      541 cattaaaatg gcaatggaga tttctgaata ggttcggtag atcgttcgca gatactccaa
      601 tatcacagct gctaatacaa cgactagcat ggtcgtaagt gtaggattaa ggttcaaaaa
      661 gaatcctaac gctacccctg ctaaagagac atgagaaagc gtatcagcca tcagcgattg
      721 tcttctgatc actaaaaaca cacctagcat cggggcgatt ccggcaatga agatagcggc
      781 aagaaacgct cttctcatga attcatatga aagcaacgcc attgagaatc ctcctttctc
      841 accagtcgga tctgtctgtc cgcatactgt ttgatatctt catgatcatg ggtgatcatt
      901 agaactgatt tcccatggac atgcgcacta tgtcggagaa gacgataaaa ttcatttctg
      961 gattcttcat ccatacctgt tgttggttca tcaagaacaa ataagtcagg gtcagtagca
     1021 aaaatacgcg ctaaactgat tcgctgcttt tgtccgccag acaattcacc tattcgccga
     1081 tttcgcatat cccacatccc tacagcttct aaagcacgct gtacatgttg atgatctttt
     1141 tcagagagac gcttgaacca gcggcctctt ggatatcttc ctgaacggac taattcgatc
     1201 accgtactag gaaaacctgc attgaaagat gcaacttgtt gaggaatata accgatactt
     1261 aatttttttc cttcttgatt tttctcagca atctttatca ttccttttga aggtttcagt
     1321 aatcctaacg atgctttcac caaggttgac ttggctgctc cattttctcc agttaaaatg
     1381 acaaattcac ccgcatctac atgataagag acatcttcta gtacaggttc atcatcatag
     1441 taaaaggtca aatcagatat ttcgatataa cgcaccttac aacctccatt ctaaaactta
     1501 attgatactt tcttttaaag ctttcagatt tttttccatg acagaaatat aattttcgcc
     1561 tgctttgatt tgttcattag tcaagctttc taaaggattc aacacttcta gcttcactcc
     1621 tgtttcattc gacaatgtct cagctacctt cgaagaggca ttttcttcaa aataaatcac
     1681 tttgatgttg tttttcttca caaattcttt tagctcagct aagcgacttg gtgtgggttc
     1741 ctgatctggt gaaacgcccg aaatcgctac ttgttttaag tcgtattgat tcgctaaata
     1801 agcaaaagca gcatgctgag tgacaaatgt gcggtttgtt gcatctttta gctcatcgga
     1861 gtatttttga tccagtgctt caagcttttt gatataagca tcggcattct tcgtaaaagt
     1921 ttccttttta tctggatatt tttcgcctaa ttcttcagca atcgttctga cttcttttat
     1981 agctaatacc ggatcgagcc aaacatgtgg atcgtattca tgtgaatgac catgcccttc
     2041 ttctgattct tcatgttcgt ctgcttcttg cgattctaaa cggtcgattc ctttagctgc
     2101 ttcgatcatc aaggtctgtt tgctatccac tgaatccttt aacgaatcca caaaaaattc
     2161 catgtctgaa ctattataca cgaaaacatc tgcatcggaa atttctgctc tttcttttgc
     2221 acttggttcg aaatcatgcg gctccgttcc agcagggatc aaaagtttca cgtttccttc
     2281 atcccctacg atctcttttg taaaatcata catcggataa aaagtcgtaa cgatcgtcaa
     2341 atcctctttt ttgtctgctt ccttgttcgt atttccacat gctgcaaaca gtaaagcacc
     2401 tatcattacc gtagctgtca aaagatattt tttcatttat atgctccttt caatgaacaa
     2461 atcggaattt tacgatttat taacgtgaac taatgtatca aaataaaaaa acacagtcaa
     2521 tagaaatcca tcaatttctg atttaagcat gtatattctt gattaaatcg taaaatggat
     2581 gagttgctgc tcatatctag ccatttcact acaataaaaa actgaagaat accattttag
     2641 gattcctcag tttttcatca cctattttac ttcttatatt ttattgcagg gttcgtacta
     2701 aataaacttc ctcacaaaaa ccttttaacc cattatatac tctctgcgct cttgaataat
     2761 gttgacacaa ggcaaaaacg gttggtccgc ttccactcat caatgcagca tctgctccgt
     2821 acttgatcat acgctctttg atttgatgga tgactggatg ccgctttccc gttatatctt
     2881 ctaaactgtt tcccattttt ttgatcatct gctgataatc ttgctctcgg atggcttcag
     2941 caagtccgtg gatgtcttga tgttggatgg cttgcgtatc tacttctcta aacactgtac
     3001 gtgtagagac actcattctt ggtttgacca acacaaccca gcactgtggc atagaaggta
     3061 atacttggat ttcttcccct ttgccaccga caaatgccgt acttccgtag acacagtaag
     3121 gaacatctgt accgacttcc aaaccgattt ctgccagttc ttctaacgaa cagcccagat
     3181 tccataattg gttgatccct cttagagcag ctgcacagtc tgtacttcct ccgcctaatc
     3241 cagctgctac tggaatattc ttcttaacaa agatcttgac accggttttg atattgtatt
     3301 tattttttag caataaagca gcttgataaa cattgttccg ttcatctacc ggtaaaaatg
     3361 ctttatttgt ttctacaatg attttgtctt ctgttatttc ttcaaaaaat agatgatctg
     3421 ccaagtctac gctcgacatc accatttcca actcatgata cccatcttgc cgtctatata
     3481 atacatccag tcctaaattt atcttggctg gcgctttttc aatcaattcc atcctctcac
     3541 ccattcgaaa agtctaagtc gcaagtattc tatcataggg aaaaataaaa gtatagctta
     3601 tgtctgccta gtggttgatt ttgtcatcgt ttcaacagct ttcacagaat tttctcttca
     3661 taatccaaac aagttctagt gattcttata attctgtgag cacttcttct gccaaactcg
     3721 gaacatttcc tttttctttt cctatcagac tgagtagctg catggtagca aatccgaaat
     3781 tacttccttt cctataagca aaataacgtc cttcccaatg atcgacatat tttaatttct
     3841 tttcataagc agactggaag gaatagaaaa aattaccata attatatacg atatgcatga
     3901 ctttttcttg aaagaaagaa aaatctgttt ctccaacatt agctaatgga gctaagccta
     3961 gattggcttc cgagtagccc attttctcgt atacaataaa cagattggct aacagatagt
     4021 cagctaattc ctcagattct ttaggtaaga cccgcaacaa atcatatccc gcctgcttct
     4081 tcgtcatcgg ctgagcggtg atgaagccaa cgactttttg atctttacgg acaataccga
     4141 caccgctaga taatacatat gatttatcaa atcgtcccac agagaagtat ttttctcgtg
     4201 caccttgaag ccatttttgt gaaacttcat ttacagcttg atgtaattct tccggcagtt
     4261 gatcgtacca agtaaaatgg tatccttctt tcatcaaacg ttgatattca agttgctgac
     4321 tcagccgaga caagtcttct gttgctagat caacaatccc tgcttcacct actttcgtga
     4381 agtgaaaacc caattcatga agcatcacta cgtagctttc tgatatctta taaaacgcaa
     4441 gctgatagcc taaacaatct gcttgtttca taaactgatt cgttgctgcc cgcatcattt
     4501 taggattgcc aactggatct cctaaaacaa agcatttgtt tcctttgatc tgataaccaa
     4561 aaacgacttg atcttggtcg tcttcttggt aataaaaata actgtaacct gacaagcgaa
     4621 gctgatggct tgtagctgtg cctccgtatg tttccaataa acggtcaaaa cggtcttttt
     4681 cgtactcact gccaagcttc gtagtatctt cagctaaata ttgataaagc gccactaaag
     4741 ctagcaatga gatgcccaaa ccaataagcc ctgagaacca cacatcatct gaaggaaaca
     4801 aaataaaatg atttcctgtt attcgaccga ctaaaaagcg tccgctgtga tagcctgcaa
     4861 ttgcgtaaac gataaacaaa aagccgaaca agctagcatc aaagattata gctccccaag
     4921 aataaacgaa ttttgttcga taaaattctt ttctggataa ccacacagct aacagaatca
     4981 agacatatac caatatcaac tgccaagata acgtacgtgt gattgtattt aaaataccaa
     5041 aagatagtaa agcaatcgta ggcagaaatg cctttttgac ttttactgaa agtgctctag
     5101 caaggccgag taataaaaag ccaaccaata gattcactgt ctgatctaaa aaattgaatg
     5161 agaacggcaa taatacttga aatagccgac tgattgcaga cagattagtg attgttgata
     5221 gaagtaccat catgatcccc gcaaaataga cagcacttac gagcacgata tgcgcaacct
     5281 tttggaaaaa cagtcgcgga atgttatcta aaaatcgatt gattttgact cctgtatgtg
     5341 tgatgaacaa tagaagtccc gtcataaaag gtagaacata ataaaacaac cgataatata
     5401 atagccaaac tactgctaca tcttgccgaa cagacaattg actcattcct aagatcatca
     5461 aaacgtcaaa agtccccatt ccacctggga ccatcgtcag cattcctatc aatgtagcaa
     5521 tcatgaacat aggatagatc gaaagcatcg aaatatctat tctcatcaac ttgccgatga
     5581 tcaaaaatac cgcaagggct cccgtccatt gtccaaagga tgccagtagc agaccaagca
     5641 agccttttgg ataaaactcg aagaacaatg tctttctctt tagataagca aaaaagaaaa
     5701 tgactggtgc taaaaaacta ccgccgaaaa gccagatcca atagcttcga aaaatacttt
     5761 ctgactgaat aaaaaagaca tcaataaatg taagaaacga ccatacagac aaaccagtca
     5821 gcataaatag tgcgactttt gatacagtgg ctaaaaccat ttttctattt gtctgctttc
     5881 cataaaaact agcccgcaga cttgctccta ccacaccacc aaaaccggcg agattgttga
     5941 tcgtgtttgt tacccacgca gcaagaagaa actcttttct ttccattttt ggttttcctt
     6001 gtttttctaa gactctgatc gtcacccaat catacaacaa catcggagtg acacccagca
     6061 gtccagccag aatcatgagc aggatcgtca tgcggctttg ttgtcccatc ctttgccaaa
     6121 tatcttgcca actcatgcca ttagcaatat ttgcgacttg atttgccaca aagactaaca
     6181 cggagccgaa aaaaatcagt tttaaaatca gactatgttc ctttatccat tgcagcatca
     6241 agtatcgttt atttttcaca ttttcccatt cctaacttgt atacttttgt gtcatacccc
     6301 aatgttatct tatttatttt aatttttcca ctttagacct gttctttgct tgcgatgata
     6361 gaaaaagaaa gatatcctaa tgctaaagga gaggataaca tgaaagtagg atttatcggc
     6421 acagggaata tggcacaagc tattattgat ggtttattaa aaaaaggaac agtctcgaaa
     6481 gaagaggtgt tcgtttccag tggacatttt gaaaatgcac aaaaatttgc tgctgaaact
     6541 ggcgtccatg catgcaaaag taatcaggaa gttgcatcac aatcagatat cgttctttta
     6601 gccgtgaaac caaaaatcat tgaaaaagta ttggaagaac taagagaata tagccagaaa
     6661 gtcttattca tttctattgc ttcaggaaaa tcattaatag atttagaaac agcgcttgaa
     6721 gatcctcagg cctccatcgt acgtgtgatg ccgaatgtca atgtcagcgt aggagcaggc
     6781 gtttctgcga tttgcaaaaa tgaagctgcc acggaggaac agcttcagca agctacgatg
     6841 atttttgaag caatcggcag cgtctatcct ttagcagaat cagatttcag tacattcatt
     6901 ggccttgctg gcagctcacc cgcattcgtt tacatgctga tcgatgcgat gggacggact
     6961 ggtgtactga atggtttgtc caaacagaca gccactgaga ttgctgcaaa agcaattatg
     7021 ggttcttgtc aaaaattcct ttcaagtgat aaaaatccat gggaacttgt agaccaagtt
     7081 tcttctcctg gcggtacaac tgttgcaggt gtagttgcct tagaagaagc aggattcatc
     7141 ccagcaatca tcaaaggaat caatacgacg atcgagaaag atcttgagat gttgaataaa
     7201 aaataatgat agcaatgttt tttcgtgcct tacaaaaaaa gaagagctgt ggcttttgcc
     7261 gcggctcttc ttttgtattt tttattattt agcataatca gttgcacgag tttcccgaat
     7321 aacgattact ttgatgtgtc caggataatc cagttcttct tcgattcgtt tacgaatatc
     7381 acgtactaaa cgaacagcgt ctaaatccga gatttcttct ggcttgacca ttacccgaac
     7441 ttcgcgtcca gcttgaactg cgtaacttga ttctacaccg gcgaagctgt tcgaaatatt
     7501 ttccagactt cttaaacgac ggatgtagtt ttctaatgat tcacttcgcg caccaggtct
     7561 tgctgctgag agcgcatctg ctgcagctac aaggacagag ataacagaag tagcttccac
     7621 atctccatga tgagaagcga tcgcgttgat tactactggg ttttctttgt acttggcagc
     7681 taattccgca ccgatctcaa cgtgagatcc ttcaatctca tggtcaagtg ctttcccaat
     7741 atcatggagc aacccggcac gttttgctaa ttggatatct tcgccaagct ctgctgctaa
     7801 gataccagct aattttgcaa cctcgaccga atgcttcaag acgttttgtc cataactagt
     7861 tctgaatcgc agacgtccga tgatcttgat tagatctgga tgaagtgtat gagcaccgac
     7921 ttcaaacgct gcctcttccc cgtattcgcg aatacgttca tccatttctt tacgagattt
     7981 ctcgaccatt tcttcgatac gcgctggatg gattcgtcca tcttgtatca gtttttccaa
     8041 ggtcatccgt gcgatttctc ttcggattgg atcgaatccg gaaagaacaa ctgcttccgg
     8101 tgtatcatcg atgattaaat cgataccagt cagcgtctca agcgttcgaa tattacgacc
     8161 ttctcgtcca atgatacgac ctttcatttc atcattcgga agggtaacga ctgaaacagt
     8221 cgtttctgaa acttgatctg ctgcgcagcg ttgaatcgct aatgataata agtttttcgc
     8281 tttgcgatct gcttcttctt tggcacgctg ttcagattct tttaccatta gagttaactc
     8341 atgattcagc tcttcttcag tcgatttcat gatgacatct cgtgcgtcct cttttgataa
     8401 accagcaatt ttttctagtt ctttttgttg atcttcaatc attttttcaa cgtctttttc
     8461 tcgctcttca attaattgct gttttgaact aagtgcttct tctttacttt caagtgagtt
     8521 ctcgcgtttt tctaacgagt catctttacg attcagcgtt tgttcgcgtt gaatcaaacg
     8581 attctcttga gattttaatt ctaacttact ttctctcagc tcactttcaa cttcagaacg
     8641 gtatttctga ttttcttcct tagcttctaa caatgcttct ttttttagag tttctgcttc
     8701 tttttttgca ctatcgatga ttccagcagc tgatgattgt gcaccagcta tttctttctc
     8761 gtgacgtgac tttgcgacca taaaccctaa accaagaccg acaattaaac cgatgatagc
     8821 gaggagaatg ttgaatacca taaaatccac ctccatacta tctttttcat ttttatcatc
     8881 tatacagctt ctatgataaa ttattctaaa ctcgtcaata tgcatcttcg catatatgtg
     8941 tacaaaaaac atgcacaact ttattctaat gtttgtaccc cgtagtgtca actttaaata
     9001 aacaaagaaa cggctttcta cttattcatt aaacgatggt ttttaaaata aatgacacta
     9061 gtttcatata ggtaaacctt taaattgaga aacttttcta ttaaattatt ctttgtagat
     9121 gaacagatat ttttatttta tgtacccact cgtataatta aataactcac gatttcttta
     9181 ttcaataaaa aaacggcagg tcaaggagag tacctactct tcagaccagc cgtttttgtt
     9241 tatcataatt tttattcttc tactaaaggc aattcttctt gtcctttttc atcttctggg
     9301 acagcgactt cttcaccaat accataagct gctctaacta gagcagaaac ttctgccatc
     9361 atttcaggat gattagccat atagatttta gcgttttccc gtccttgacc aatccgatct
     9421 tctttataag aataccaagc accgctttta tcaacaatgt ctttttctac tgccatgtct
     9481 agaagttctc cttcttgtga gatccctaaa ccatacatga catctacttc agcgattttg
     9541 aaaggaggtg ctactttgtt ttttacgact ttgatctttg tacggttacc aacaatatct
     9601 gtaccttgtt tcaattgttc tgcacgacgt acttccaaac ggatcgttgc gtagaatttc
     9661 aatgcgcgtc ctccaggagt gatttctgga ttcccaaaca tcacgcctac tttttcacgg
     9721 atttggttga tgaaaatagc gattgttttt gttttgttga tcgaaccaga gagtttacgc
     9781 aatgcttgag acatcaaacg tgcttgtaac ccgacatgtg agtcacccat ttcgccgtcg
     9841 atttctgctc gtggaactaa agcagcaact gaatcgacta ctacgatatc tacagcccca
     9901 cttgatacta aagcatcagc gatctctaga ccttgttctc ctgtgtcagg ctgtgaaaga
     9961 agtagttcat cgatattcac acctaatttt tgtgcatatt gcggatctaa cgcatgctca
    10021 gcatcaatga aagcggccgt tccgccattt ttttgtactt ctgcaatagc gtgtagtgca
    10081 actgttgttt tacctgaact ttcaggtcca tatacttcga tgatacgtcc acgggggtaa
    10141 ccgccgacac ctaaagcgac atctaacgca agagagccac ttggaattgt agagatttgt
    10201 tggtcgattt tttcgcccaa tttcataata gagcctttac catagctctt ttcaattttt
    10261 ttcagtgcag catctaaggc tgctttacga tcatctgcca atacgataat cctccttata
    10321 tttacaaatc agagcaacat ttcattatcc ttatcatatc ttttttagtg aaaaaaagca
    10381 agaaaaaacc gaacaaatat tcgcatttat cattttctta aaagctgttt tctcaaaaga
    10441 tcaaatcctt gcataaccgc attatatctg atagcagcac gatctctggc aaagtgatat
    10501 ttttccgaaa ccacgccgtc tttagatgaa atagcgatcc atactgttcc tgcagattga
    10561 ccttctaacg tgtccggtcc agccactccg gtgaaagcta cactgtaatc tgttcttgct
    10621 atctcacgtg cttgaatggc catctgctcc acacattctt tgctgactgt tccatattct
    10681 gctaatagct ccggatcaat ctccagaaaa ccagcttttg tttggagcga gtaggtaacg
    10741 aatccgcctg gaaatacttc agatacacca gcaatgtcgc ctaatgcggc ttggaaggcg
    10801 ccggctgtca aactttccgc tgctgttaca gtctgcttgt tttctttcag tagttccact
    10861 acgacttttg ctagcgaatt atcatcgccg tatccataaa aatattcccc tactcgttct
    10921 tggatttttt cttctaatgc taaaagagct tgattccctg cctgaacatc atttgttttc
    10981 actgtcagtc gtaatgtcac ttcatttggt ttggcatatg gtgcgatcgt tggattgatc
    11041 tgtgtctcaa tcagatcttt tagctcagtg accagtcgcg attcaccaat cccataaaag
    11101 cgcaaaactt tagaaattag tttttcttca gaaggaaaac gttcctctag taaaggacgt
    11161 acttgttcca caaacattgg tttcaactca ttaggcggac cgggaagaag gatatacgca
    11221 tgtgtatctg tttgataaaa agtccctaaa gcaagccctg tacgatttgg tagaggaacc
    11281 ccgccttcga taatctggga ctgttgcaga ttgtttttgg tcatttttcg atgggttgtt
    11341 tcaaaatatg ccaataattt tttgtatcct tccgtatttt gtatgagaga cttaccgagg
    11401 tgagccgcag ttgcttcttt tgtcaggtca tcttctgtcg gtcctaatcc gccacataag
    11461 agtatgagtt cacttcttgt ttcagctaga gcaatcaact cttctaaacg ctgctgatta
    11521 tctcctacaa ctgtttgata ataaacttcg atacctagat cagctagctg ttctgacaag
    11581 aaagtggcat tcgtattcac gacttgtcct agtaaaagtt ctgttccgac agctattatt
    11641 tctgctttca ttacttcccg ctcccttata tagtatacgc tgtattttta tattttttta
    11701 tttaaaaaga gggcaaggag ctgtgacaaa aatcttgtca cagctccttg ttccaaataa
    11761 acagtgttct cctgccaaaa cctcggtcat aagtcacaat atacataaaa catgaaaagc
    11821 tgttttttga aattatccct tgtgactcag agttagacgg caggctcaca acctctccac
    11881 taaacgatac ttacattgat cctttgaaca caccgctgtt tttgatgaag taatccacac
    11941 ctgaatagat agtaaagatc agacaagcat aaagcatgat ttgatctact ggaataccga
    12001 tcaggctgaa tgggatatta ttgataaaca gcaagataat agcaaccatt tgtgtagccg
    12061 tcttgacttt tcccggccaa gctgcagcca tgacttctcc atgttcgaca agtagtaaac
    12121 gcaagcctgt gacggctaat tcacgacaga caatgatcgc tactacccaa ccaggggcct
    12181 ttccttggtc aaccaacatg ataaaagcag tcataactag cattttatct gccaacgggt
    12241 ccgcaaactt gccaaaatta gtgacaaggc ctcgcgcacg ggcaattttt ccatctaacc
    12301 agtctgtgat actagctaaa gcaaaaataa ctgcacctac tagctgtgtg acagccagtg
    12361 tggtgtctcc tacgttaaat tgtccccaat ctaaaggtaa acctacaacc aaaatgaaaa
    12421 tcgggatcat acaaattcta agtacagtta atttatttgg taaattcaca ctatcgactc
    12481 ctttcttccc tattctacgt gactatcagc gaagtttcca ccgatattat ttacttttcc
    12541 aaatgaattt attcaaagga gtatttttat tgaaattgat ctttttgaat taacgaagaa
    12601 ggaccgcgac acagatttgt cacgatcctt cttttttata cctacaagta aacagatgcg
    12661 taaactaatt tatacgatca ttctgcgtat tgaagagtaa ggttcacatt ttttggactc
    12721 tgattgtttt cccctggatt aactggtacg gattctccat tcgctttgat cgttgcattg
    12781 cttgctgcac caatcgtgat ggtcgcattt gttgctgttt ccggtaacac ggtactctgt
    12841 gtttcatttg ctgccagtgt cccttgataa acataggcat tgtctactag cacacctaac
    12901 catacacgat tgacagcagt aaattcaagg gttacaggct tggtagcgtc tgtgatattt
    12961 actgcgatgg cactgccaga atcttctcct gtagctattg ccattttctt ttcttcactt
    13021 gattttgttg ttgattctac tgtagaagac tctttggtct gcgtgctttg ctctgaggaa
    13081 gaggactcct cgactgatcc ttctacttta agcgaagaag ctgtgtctcc gattattgga
    13141 tcagcttgac gatcttgcca agtcatatag cccacgacaa cgacaatcgc caaagcaacc
    13201 aatcctaata aaatgacagg aagacttgtc caaaagcgac ttggatgcga ttcttcctga
    13261 tgcattgctt ttctagaagt acttactgtt tcaggctgcg gacgttcagg gaccgatcct
    13321 gtcaatttgc ttttcccatc aaaaacatcg actaaatggt ctccatcttc tcctacagct
    13381 tcagcgtatt gccgaataaa tgcccgcacg taaaatttac caggcaattg gtcaaaatca
    13441 tcattgtcga ttgcttccaa ataacgtttt tgaatttttg taatctgctg taattcatct
    13501 aaagatattt ttttattcag gcgtgcttgc cgtaattttt ctccgatggt ttcaaaagcc
    13561 actcgttcat cttctccagt ctgtttgatt ctccgaagca tctgctgttg caacgaaaga
    13621 cgttatgttt gggtgctgtg acaaaagtca tgtcacagct ccttttttcc gaataaacat
    13681 tgttctcctg ccaactcctc ggtcacaagt cacaatatcc aataaatata agaagctgtt
    13741 ttttgaactt gttccttgtg actcggagtt aaacggcagg ctcacgacct ctttcttaaa
    13801 cggtgttcaa tcagctgttc ctcggtatta gctcaaaaac cggcaaaata taaggagata
    13861 tttttccgta tgtttctcta atactcaggt caaaacgctg atttcacaac ctcttcctta
    13921 aacggtgttc aaaagcttgc atctgcggta ataagtccaa ccaagttaaa aacattaaga
    13981 agctgttttt acttggctgt tcttattact tgatgcaggg cagctttttc acaacctctt
    14041 ggtacacggt gttccagaag tagcttcttc ggcaataagc cgaaatatca caaaaatttg
    14101 agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct gaccacttct gtcacaacct
    14161 ctttgattac ttcgtttgat tttattttaa tataagttga ttaaaagaga gcatgatttg
    14221 ttttattttc tcactaattt ttccaacaca actaagaata ccataaaagc tgattttgga
    14281 ggaggttcct actacaagtc taaaacgaaa tttaagactt ttcctcataa aatactggtt
    14341 tattttggtc tcatgtaaaa ctcgctgaag gcctcttcac ttataaatgc ttccccagct
    14401 gccagtacgt ctttcatttg gatcgaatcg atgatttcag gaagatcaaa taaagttcga
    14461 tcgccaaata aactttgcgt aaattgattg gcaatatatt cgatcgaatt taatgattgg
    14521 aaatattgtc caagcatttt cttcttcagc aaatcgagat ttgtttctga cacttccgga
    14581 tcatcagcga atccaagaat gatttctttg actttttctg ctgctttctc tggttcgtcc
    14641 gtatctccgc tgaagtccgc aaagtgaaat tcacgatcca agctgaattc aaaaccaaag
    14701 ctgtcatcga taattccttg attgtacatc gctagatagt ttcgagaagt attgcctaaa
    14761 atcatttgga acaacagatt gattgctgtt ttatagcgta acagttctgt tccttcttgc
    14821 ggcaatgtgt ctaatccttt aataccaagt acaaatttat ctcgtgtaat ggctgcttcc
    14881 aatgcacttt gtttgatgat ttcttttgtg ttttcaggga aataacgtac aatttcttgc
    14941 tttggaggga aattttttgc ttcttggttt tcccgaataa gtttcatcaa tttctcaggc
    15001 tccatcttac caactacaaa taaaaccata ttgcttggct gataaaacgt ccgatagcac
    15061 gtatacaagt cttgagctgt gattttgtcg atactttcaa ccgtgcccgc aatatcgata
    15121 tgcaatgggt gtgtaggata caagttattt agtatgccaa aaaacatccg ccagttaggg
    15181 tcgtcttcat acatttggat ttcctgaccg atgattcctt tttccttgtt tacagtttct
    15241 tctgtgaagt aaggtgcttg tacaaaatcg atcagagtcg tcagattctt ttctacctga
    15301 tccgtcgtag aaaacagata gctggttttc gtaaaacttg tgaaagcatt ggcggaagct
    15361 ccttgtttcc caaataattg aaagacatca ccatcttctt tttcaaacaa cttatgttct
    15421 aaaaaatggg caattccatc tggaactttc accttttctt tttcaccata gggaataaat
    15481 tcgttatcga tcgaaccata attcgtactg aacaaaccat atgtcttgtg atagtcattt
    15541 ttaggcaaca aatacacggt caaaccgttt ggcagtactt catgataaag tgtttcattg
    15601 atttgctcat attctgtttt attcatttgc atttcctcct gctaaaaaga agattgcctg
    15661 caattcgatt tgctcagcta cttgttgtat ctctttgatc gttacgctct ccatacgttt
    15721 caaccattct tcatcagata attttgtttc cggcagccag ctgtccaaaa aagaagcttc
    15781 gattgccgct tgcggagagt ctagagaaag cagatattgg tttctcagca ttgcttttgt
    15841 ctgagacagt tccaactctg ttatttcacc atttcgcaaa ctttctaatt gctcgtgaat
    15901 cagatgaagt acttgatttc ggttcttttc atcgataccg gtttggacac tcatgaatcc
    15961 tcggaacgta tccacgctac tagaggcata ataagctaga ctttctttct ctctgacatt
    16021 catgaaaagt ttagagtgag gaaaaccgcc aaataaaccg ttaaagacca ttagcgcaaa
    16081 acgttctggt tcatcgtaat acacatttgt ctgataagct aggttcagct ttgcttgaac
    16141 gattggctca cgtacttgtc gttccttcac gatattcact tgaggttggg tataaaacag
    16201 atctggataa actgtttccc gcgactcaaa tggtaattgc ccaactgctt cagctgcttt
    16261 gtccggatca acatcgccga caacaaaaat gtcgatttga tcatttgcca tcatttcttg
    16321 atagtaggac gctagcgaac tagcagtgat cttgtccaaa tcttcgacta cgcccaaccc
    16381 tgggatcttt tgttccgggc tgttttcaaa ataaagttct tgcaaggcat aggaagcaaa
    16441 agtttgttta tcttccttca aactttccaa atatagacgc agattgttct tttccaagtc
    16501 aaacgtcaac tgatcaaact gatgattttt aatatttgga taaaataaaa cctcttttaa
    16561 aaaatctacc gcttgagaaa aaaggtttgg atcattgaca tattcaccat tcacaaaact
    16621 gatccctgcg tttagccaat gtaaatttcc tttttttcta actgataatc caaagcttgc
    16681 gccataaagc tctgcaagtt gcgcacttaa ctttgtctga tccgaataat tcaaactatt
    16741 tgtttccaac atgctgctga gaagtgaacg tttcgtgatg acttcttgtt gcagacgcgc
    16801 agtaaaacgg ataaaaatcc ggatcgtttt gtattgtttt gttggtaaga catgaagatt
    16861 cacgccttct gccaattgat acaccatatt tatatgctcc ttttcacagt tgattgcccg
    16921 cttactcttt taatgggtca acctgccttt tttacttatt cctctgcttg aaatttataa
    16981 gttcaggcaa ttcgccgttt tttctaaatg atgttacact ttatagtata gcatagatgt
    17041 tttttatcaa aagaaacaga cggctaaaag cgtatattgt ttttttattt tttatttttc
    17101 gatatactaa agacaaataa aagaagggag ggtcattcat gatcaaagaa ttcaaagagt
    17161 tcatcatgcg tggagatgtc ttggatcttg ctgtcggcgt tgttattggt agtgcgttta
    17221 caagcatcgt aaaccaagtg gtgcaaggct tgatcacacc actaataggt tggtttgtcg
    17281 caatgatcac aggtacttct gatttagaag gagcactttc aattcttgac tggtcgccga
    17341 caaaaggcgt cacatttgcg tttggtaacg ttattagtgc tatcattaca tttctgatca
    17401 ctggttttgt cttattctta gttgtcaaag cggcaaaccg tgcgaagaat ttacgctcca
    17461 aagaagagca agtggaagtg gaagaaactc ctactgcaga agattatctt agtgacatac
    17521 gcgatcttct tgccaaacaa gtggcagaag acaatacaca tccagtagag caaacaaatc
    17581 tttccgatac ttctaaataa taaagaatct aaaaaaaaga aagccgattt tcagtgtcgg
    17641 ctttcttttt ttagattctt aacttgctac ttgttccttt ttcatgtcga tatgtggaat
    17701 atcatcttcc aaatacacat ctgagattgc cttgaatcca aacgactcat aaaaagcttg
    17761 taggtattct tgtgcgccga tcacgattgg tttttctgga aaatttttct ggatcatgtt
    17821 aatcgtttct tgaacaatct ttcttcctaa accatcttta cggttgtcct gatgaacaag
    17881 gacacgtcca aaatggatac tctccgcttc ttcatatact cgcgtgtagg caaggatctt
    17941 cccatcagta tcttgaaaaa acgtatgcca agcttctcca tcaatgtcat caacttcttg
    18001 ataaggacac tgttgttcca caacaaaaac tgcgattcgt tctttgacga tcgcgtaaaa
    18061 ctctgctgtt gacaattctt caaatttctt tacctgatat tccactaagc aaacgccccc
    18121 tgctttttta ttcttagcat accgattcgc acacaaaaaa gcaaacgaac cttacgaatc
    18181 tcaggctcgt ttgcttttta aactgcttca gttaaaaatt cgatctcaac tgtccgagta
    18241 agtacatctg aatagctgta agatactcgt tcaaaagaat tttcttcagg atccaaatca
    18301 acaacgaata ctgaaggata ggtttctgtt aaaacgccgc ggcgttctgt ttgacgcttt
    18361 cttcctgtct gagcaaccaa catgattggg ctgccgatac gaccttctaa atctttcttg
    18421 attgaagcta aagttgttgg catacagttc acctctacta tcaactatac cacatatttt
    18481 taaaattttc aagtttacca taaaaaaaaa agaaatgcaa taaaaataaa taagaagctc
    18541 attaagattt ttctaaatta ttcctcatta taaaatttca agtgtttttt tattttgagt
    18601 tttactctcc cgtaagcgct cattacttgc ttgtgatcca ttgctaaaca ttcagccatg
    18661 tctacaaaat caacgccttg catataataa tgaaaaattt tagcttctat ttttgaaaac
    18721 tggatttcgc aattttcgat tgcttctttt aataaaagat gctgaattgc taaaggttct
    18781 tgcaatgtga tctctgctaa aaagtctggt ccttctgtct ctattttttg gtcgattgag
    18841 acttcatctt tttgatcacg acgcttcagc gcgttctgct tcctcaacag agaatagatt
    18901 tgattgctta taacgctttt ataataactt ccaaagctaa cacctttgct tttttcgtaa
    18961 gttctcaatg cctgatataa agcgatcctt gcttcttgtt cccaatcctc agaaacataa
    19021 tttcttaaat aataggtgtt tttcaaacgt agtaaaagtg gaaaatattt ttgatacagg
    19081 gcagcaaatg cttcttcgtc accattacgt gcagcgcgca ataactcctc agacataaaa
    19141 taattcctcc ataaattcgt ttatggagga atcttatcat caatgattca tcgaactgat
    19201 tagaacgata tgttatttat tttttctcat taaatcgtcc aatttttggg acaatttttt
    19261 taactgctct tcattccatg gagaattacg tcggaaatca ataaaatgca tatctttatt
    19321 ttcttgggct attgtttttt ccgttttttt tatcgtctta tataattcat aagcagaagt
    19381 tcgtaatgct ccttgggaaa agatcatcca ctgttctgct aaatcgcttg tcgccactgt
    19441 cacttgtgtc aaacggtcat tcagctgacc agcgattctc tcgatataac tgtcagccgt
    19501 ttcgtcttct ttcgtaaaaa caacatccaa ctggtatttc ttataatttt gttggatgcc
    19561 cggaactagt tgggcatcga agacaacgat gacttccatt ccttcatatt tcgcataatt
    19621 tgataaacga tgtaaaagag cttctcttgc atccgccatt ttgtcctgct ttttaagctg
    19681 gacaagttcc ggccacgaac caatcatgtt gtagccgtct acaatcaaca gctgtttttt
    19741 catggtctac tccccctgac gttgggaaaa agcctgatac ataagcaaac cagcagcaac
    19801 actggcattt aagctctgga cgtgcccggt catcgggatc gtcagcaatt catccatttc
    19861 ttttttcaat cctggactca ttcctttgcc ttcattacca atgactaaag caattgatcc
    19921 tttggcattc catttttgga agtttgtgcc ggtcatatct gtgccaaata cccaaaatcc
    19981 tgtttctttg agctgttgca cagcttgtac caaattcgtc acgcgtgcaa cagggacatg
    20041 ttcgactgcc ccagtggaag tctttgttac gataggtgtg attccaactg ctcggtgttt
    20101 tggtatgatc acaccatcga cgccacttgc gtctgctgtc cgtaaaatcg acccaaaatt
    20161 gtgtggatct tcgatgctat ccaaaatcaa aaagaagggg tcttcttttt tctcttttgt
    20221 tcgttcaatc agttggtcaa gggataaata ttcataaggt gtaatcgcaa gaacgactcc
    20281 ttgatgcacg ccgtgatcgc tcaaagtttc taatttttgt ttaggaaccc atttgactgg
    20341 cacagcttgt tcacgtgcga tttctttcag ttcttctact ttttctcctc gtgaatcttc
    20401 ttgaagaaag agtttattcc ctcgcccttg ttgtagcgct tctactgcgg catggtgccc
    20461 aaagacgaaa ttatcttcta atgcttcttc attttgatca gaaactgact gatttcgtcc
    20521 agtttgcttt ttatttgaat atctattttg ttttttgtct tcaaatccac gtttcgattg
    20581 atgtttgttt gttctttctt tacccatttt tttcaccaac tttctgaata caccagcgga
    20641 tcaattcttc catccgctcg gtttgttttg tcaaatgcag atagcccatg agcgcttcga
    20701 accctgttgc gacccgatac gtcgtaacat cagcattttt tgcgctcgta tggcttttag
    20761 catttcttcc tctgcgataa taaagttctt cttcttctgt caaaagatgt tcttccagca
    20821 tttgctgaat caaaaaggct tgtgcattcg cagaaacata attcgttgcc gcacgatgca
    20881 gcttattggg ttttgtttct ccttgatcaa tcaggtggtc acgaacatag atttcgtaaa
    20941 tcgcatctcc aacataagcc aaggctaaac cgttcaattg tgtgtaatct ctcatgcttc
    21001 tcttctccat cgggttcctt gcggtgtatc ttctaataag atcccttttt ctttcaacaa
    21061 atcacgaatt tcgtcgcttc ggacaaaatc acgattgctt cttgcttgat ttcgttcttc
    21121 gatcaattgt tcaatctctt catctaataa ctcttctgct gtaaataaaa taccgaagat
    21181 cgaaagccat tgcgagaata aagcttctcc ttgtgccaag acagcggcag aaactttcgg
    21241 ctggtctaga taagtgttca gccacttagc aagctcatag accactgtga tcccatttgc
    21301 tgcattaaaa tcatcgtcca tttcttcgat gaatcgctgt tctaaatttt ctaacaaagc
    21361 gattttatcc gcatcatctg ccaaattctc tgaggcagtt tcttggcgga atttcaaatt
    21421 gtcaaacgta ttcttcagtc gttgtaaatt ggtggctgct tctttcatcg tcgtttcact
    21481 gtagcggatc ggacgacggt attgtgtggt cgccatgaag aaacgcaaga cttgtggatc
    21541 aacgtttttg atcatttcat gtacagtgat gaaattccct agagatttac tcattttttc
    21601 atcgtcttcg ccaatcgtta catagccatt gtgcatccag taattagcga attttttccc
    21661 tgttttcgct tcactttgtg cgatttcatt ttcatgatga ggaaattcta aatcttgccc
    21721 gccaccatgg atatcgatcg tttctcctaa atgttttgtt gccataacgg aacattcaat
    21781 atgccaaccg ggacgtcctt tgccccatgg tgaatcccaa gaaatctcgc caggttttgc
    21841 ttgtttccat aaagcgaaat ctaatgggtc ttctttttgc tgctgttctt cccctgtacg
    21901 ctgacttgct ccgacttcca gttcatcgat ggattggtca cttaatttcc cataattttc
    21961 aaatttacgg gtacggtaat acacatcacc tgaagattca taagcatagc ctttttcaac
    22021 caacacttga ataaatgcta aaatatctgg catatgatcc atgactcttg gatgcagtgt
    22081 agcaggttcg acattcaacg ctttcgtatc ttcttcaaat gctcggatga aacgatcagc
    22141 aacttctggt gctgtaatac ctaattcgtt tgctgtacgg atgattttat cgtctacatc
    22201 cgtgaaattc gatacgaaat cgactttata gcctcgatat tcaaaatagc gtcgaattgt
    22261 atcgaaagca atggtgctac gagcatttcc gatatggata taattataca cagtcggtcc
    22321 gcagacatac ataccgacat gtcccttttg tatagggatg aattcttctt tttttctggt
    22381 caatgtattg tagattttta tcattctgat ctcctcactt tttctccttt gattcgaacg
    22441 acttttgctg gtactcctac cgctgtagcg tcgtctggaa catctttcaa cacaacggca
    22501 cctgcaccga ttttggcgtt ttttccgatc gttaccggtc ctaatatctg cgcattcgca
    22561 gagagcatgg ctccttgttt aacagttggg tgtcttttac ccgtttcttt tcctgtgccg
    22621 cctaatgtta cgccatggaa taaaatcaca tcgtcctcga tttctgcggt ttcgccaatg
    22681 acgattccca ttccatggtc gatgaacacg ccagttccaa tcgttgctcc tggatggatc
    22741 tcaacaccag ttaggaaacg ccagaattga gcattgatct tcgccaataa aaagagccgg
    22801 tgctgatata aaaaatgaga gaaacgatgc caaaacaagg catgaaaacc agggtatgtc
    22861 aaaacgatct cagctgtcga acgtgcagcc ggatcgtttc tctttgctgc atcaatcgct
    22921 cgggtaaacc aactcatgac tttccactcc tttcgtaaga attttatgta ggaaatcatc
    22981 ttcttataaa gaaaagaagc gccttttaaa taagtttcct tatcaaaaga cgctttccgc
    23041 gtggttccac ttttcttcat aatcaatgat tatctctgac agctaacgcc tgttatacgt
    23101 tcttggctac tttgtttcct ataaaacagt ttttcctgtt ttctgtgttt cgccaagacc
    23161 actcccagag gcatttcggc aaccattcat tgataccttg caccacccgg tatctctctt
    23221 gtaatgatca gctgcttact tttttctgtt catcgtgttt agttcagtac ttgatttaaa
    23281 tgtgctaatg atttttcacg accaagcaat tcgatcgttt caggcaattc tggaccatgc
    23341 atttgtcctg atacagctac acggattggc atgaaaagat tttttccttt tacgccagtt
    23401 tctttttgaa cagctttgat ggcagctttg acagttggag catctactac ttccatttgt
    23461 tctagctgtg ctttgaatgc gtggagaaca gtaggaactg tttcaccagc aagtacttct
    23521 tttgctgctt cattcaatac aggatgttca ttgaaaaata gcgtagacag atcaacgatt
    23581 tctgcagcat agctcatttg aggttgatac aggctaacga tcttcttcac ccattcgatt
    23641 gtcttttcat ctgggttttc tgctacacgt ccatcagcga ttagataagg cagacacatg
    23701 tcagtaaatg catctagatc cattgctttg atgtaatggt tgtttaccca ttgcagtttt
    23761 ttcgcatcga aagctgcggg tgatttactt aggcgttttg gatcgaacat tttgatcaat
    23821 tcttggtgtt cgaagatttc gtcttcgccc acaggtgacc agcccaataa tgcaataaag
    23881 ttgaacattg catctggtag atagcctaat tcgcgatatt gttcgataaa ttgcaagatt
    23941 gattcgtcac gtttgcttag ttttttacct gtttcactgt tgatgatcaa tgtcatatgt
    24001 ccgaaggttg gtggtgtcca gccaaatgct tcatagatca tcagttgttt tggtgtattg
    24061 gcaatatggt catctccacg taaaacgtga gtgattttca tcaaatgatc atcgaccgca
    24121 acagcaaaat tataagttgg cataccatcg cgtttttgga tgacaaagtc gccaccgata
    24181 ttatcggatt cgaacgaaat agcgcctttc accatatctg taaatgcgta ttctgtattg
    24241 cgaggcacac ggaaacgtac aacagattct agaccttgtg cttctttttc tgcttgttct
    24301 tcaggtgtca agttcgcaca tgttcctgcg taatgaggca tttctcctct tgcgcgttgt
    24361 gcttcccgtt ctgcttctaa ttcttcttct gtgcaatagc atttgtatgc tcgattgcta
    24421 gcaagcagct gatcaatcag cggttgatag atttctttac gttcagattg acggtaaggg
    24481 ccgtattctc caggattttc aggagattca tcccaattca ttcccaacca agcaaggttt
    24541 tccaactggc ttttttcgcc atcttcgata ttacgttttt ggtcagtatc ctcaatacga
    24601 ataataaagt ctccatcatt gtggcgggca aacaaataat tgaataatgc agttcttgca
    24661 tttccaatat gcaggtgccc agttggactt ggtgcatagc gtacgcgtac tttcgtcatt
    24721 ttcttcctct tctttcaaac tagactttga ctaacagcaa aaattccgtc agttacggct
    24781 tctcaaatat catttattta aagccactca gcacaaattt taccttttct aaggtaaata
    24841 gtaaagccaa ctggttgaat ttccttacca gttggctgaa tttctttttt attttgattg
    24901 tttagtcttt ttctctgtct cttttttatt gtcgattcct cgtcctgcat gtgcaggttt
    24961 agcaaagatc atgcgtccag cagctgtttg aagggcactt gttacaacga cttcgatacg
    25021 ttcattcatg taatgctgtc catcttctac gacaaccatc gtgccgtcat caaggtaggc
    25081 tactccttgt tggcgttctg tcccggcttt tactactaga acattcatcg tttcaccagg
    25141 gatgacgact ggttttacgg catttgccaa cgcattgata ttaagaacag gtacattttg
    25201 gaattcagag actttattca agttatagtc attggttaca atgacaccat ctaaaagctt
    25261 agccaatttg atcaatttgc tgtctacttc actgatatct tcaaagtctc catcgtacat
    25321 ttcgacagat ataccctctt ctttttgcaa ggcatttagg atatctaatc cgcgacgacc
    25381 acgcacacgt ttcaaactat cccctgagtc cgcgatgtat tgtagttcgt aaagaacaaa
    25441 attagggatc atcaaggttc cttctaagaa tccagtcttt gtaatatcat aaatccgacc
    25501 gtcaatgatc acgcttgtat ccaagatttt atatttatgg aaattttctc ctgattttcg
    25561 ttctaacagt tgactctcaa cttgactagc tgtttcttgc tcgatttttt tgcttcgtga
    25621 accaaaaact ttcttccatt cttcgattcg tgtcgttccc attctcaatc ccagatagcc
    25681 taagatgatc atcagtaaaa tcggcaatac actattgacg aatggaattt ccatattgta
    25741 aagaggggtc gagatcacaa cacctaaagt caatccgatg atcgcaccga tcgcaccgaa
    25801 gagaagataa gtcaagctga cctcacttag tttttgttct acttgtttga taatagctga
    25861 gatatgtttg gccaacagca aagataataa aaagaaaata agtgcgccta acaggctgtt
    25921 ggtaaatgta ttattcagcc atccgtttga ctgttgaccg attgcctgcc aagctgaggg
    25981 taaaaatgaa atacccaagc ttgcgccgac aatgatcatc agcaaagtaa tgacacgttt
    26041 ttgcatcgtt tttcctccgt ttcgacaatc atttattagt tagcggaaca cacgcttcaa
    26101 cgtttcagcc aaagttgcta ctccaactat ctcgattcct tttggcgctt cccagttacc
    26161 taagttgttt ttaggtaggt atacttttgt gaagccgagt ttttggactt ctttgactcg
    26221 ttgttcaata tgactgacac gtctgatttc tccagtcagt ccaatttcac cgatgaaaca
    26281 ttccgttggc tgtgttcctt tttctttata gcttgaagca atactgacag cgatagctaa
    26341 atcaatcgct ggttcattta attttacccc acctgcagct tttaagtaag catcttgatt
    26401 ctgaagaagc aacccagctc ttttttccag cactgccatg atcagagata cccgattgaa
    26461 atcaagacct gttgttgttc gtttggcatt tccgaacata gtaggtgtga ctaatgcttg
    26521 gatctctacc aaaattggac gtgtcccttc cattgccaca acgatagcag agccagttgc
    26581 accgtctagg cgttcttcta agaaaatctg tgaaggatta gcgacttctt ccaatccatg
    26641 ctcatgcatt tcaaaaatcc caatctcatt cgttgagcca aatcggtttt ttactgctcg
    26701 caaaatccga aaactatgat gttgttcacc ttcaaaatat aacaccgtat ccaccatatg
    26761 ttctaacatt cttgggccgg cgatcgagcc ttctttggta acatgaccga cgataaaaat
    26821 ggctatccca tttgttttag cggttttgag tagttctgca gtggtttctc ttacttggct
    26881 gacacttcct gccacacttg tgatgtctgg ctgcgtcatc gtttggatgg aatcaatgat
    26941 aacataatca ggggagatat gttcgatggc acgactgatt tcgttcatat ctgtttcagc
    27001 gtatagataa aattctgtgt tgatacttcc gaggcgttcc gcgcgtaatt ttatctgctc
    27061 tgcgctttct tcgccggata catataagac ttttccgcct atggctgcta attgctgaga
    27121 aacttgtaac agcaaagtgg atttcccgat acctggatcg ccaccaatca agaccataga
    27181 ccctggcacc acacctccgc ctaacacacg gttcagttct tctaattttg ttttgatccg
    27241 aggttctttt ttagggacga cgtctgcgat ttttgttggt tttgcttttt ctcctgtcaa
    27301 acttgtccga gttctgcggt ccgttgtatc ttgttcgatt tcttcgacca tcgtattcca
    27361 ttttccgcaa tttgggcagc gtcccaagaa cttaggcgac acatacccgc aattttggca
    27421 aacaaattgt acttttgatt tttttgccat tgtaccgcct ccttttatcc taatcatgag
    27481 acattaaatc aagcttaagt atctttaacg ttttatttac tggaagaacc aaatccgcct
    27541 tgacgttgag cagtcgtttg atcttgatct gcttttaaaa acggcaaaaa gatcccttgg
    27601 ccgatacgct cgcctttttt gatcactaca tccttgaaac caaagttggt gaattggaac
    27661 atgatatggc cttcatttgc ctcattattg taataatcac tgtcaatcac acctactccg
    27721 tttgtcaaca ctaaaaaccg cttcaacgga ttgcttgaac ggttagcaag ctgcaaaaat
    27781 tcatcttctc ccatgtaagc tttgattcca gtaggcacga gggttggttt caattgtttt
    27841 tcattttctg atacatcttt tttcaatcca tctgcgactg tttccaaccc ctttttccag
    27901 cctgctttcc agatacttgg aatcacgacc tcttctgctg cttcaaaatc atagccagca
    27961 gcatgatgtg tagcgcgctg cggtagattg atattttgat ctttatattt agaaataact
    28021 tcaaatcctc tttttctcac cggttttcct
//
LOCUS       DADVXU010000027        27807 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.029, whole genome shotgun sequence.
ACCESSION   DADVXU010000027 DADVXU010000000
VERSION     DADVXU010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 27807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.029"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(263..1207)
                     /locus_tag="KB953_001112"
     CDS             complement(263..1207)
                     /locus_tag="KB953_001112"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBB6866100.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1503..2447)
                     /locus_tag="KB953_001113"
     CDS             complement(1503..2447)
                     /locus_tag="KB953_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6866101.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2668..3882
                     /locus_tag="KB953_001114"
     CDS             2668..3882
                     /locus_tag="KB953_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB6866102.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3904..5919
                     /locus_tag="KB953_001115"
     CDS             3904..5919
                     /locus_tag="KB953_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HBB6866103.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6002..7375)
                     /locus_tag="KB953_001116"
     CDS             complement(6002..7375)
                     /locus_tag="KB953_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBB6866104.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7401..7685)
                     /locus_tag="KB953_001117"
     CDS             complement(7401..7685)
                     /locus_tag="KB953_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBB6866105.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7687..9702)
                     /locus_tag="KB953_001118"
     CDS             complement(7687..9702)
                     /locus_tag="KB953_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBB6866106.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9881..10186)
                     /locus_tag="KB953_001119"
     CDS             complement(9881..10186)
                     /locus_tag="KB953_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HBB6866107.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10275..11306)
                     /locus_tag="KB953_001120"
     CDS             complement(10275..11306)
                     /locus_tag="KB953_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBB6866108.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11513..12388
                     /locus_tag="KB953_001121"
     CDS             11513..12388
                     /locus_tag="KB953_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBB6866109.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12540..13382)
                     /locus_tag="KB953_001122"
     CDS             complement(12540..13382)
                     /locus_tag="KB953_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBB6866110.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13474..15351)
                     /locus_tag="KB953_001123"
     CDS             complement(13474..15351)
                     /locus_tag="KB953_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBB6866111.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15348..16769)
                     /locus_tag="KB953_001124"
     CDS             complement(15348..16769)
                     /locus_tag="KB953_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBB6866112.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16772..20161)
                     /locus_tag="KB953_001125"
     CDS             complement(16772..20161)
                     /locus_tag="KB953_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBB6866113.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20418..21809
                     /locus_tag="KB953_001126"
     CDS             20418..21809
                     /locus_tag="KB953_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6866114.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21882..22529)
                     /locus_tag="KB953_001127"
     CDS             complement(21882..22529)
                     /locus_tag="KB953_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBB6866115.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22537..23223)
                     /locus_tag="KB953_001128"
     CDS             complement(22537..23223)
                     /locus_tag="KB953_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6866116.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23229..24302)
                     /locus_tag="KB953_001129"
     CDS             complement(23229..24302)
                     /locus_tag="KB953_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6866117.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24524..25198
                     /locus_tag="KB953_001130"
     CDS             24524..25198
                     /locus_tag="KB953_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBB6866118.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25203..26543
                     /locus_tag="KB953_001131"
     CDS             25203..26543
                     /locus_tag="KB953_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBB6866119.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26692..27678)
                     /locus_tag="KB953_001132"
     CDS             complement(26692..27678)
                     /locus_tag="KB953_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HBB6866120.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8110 a         5249 c         5048 g         9400 t
ORIGIN      
        1 atgaacgaat tagatatgaa tcattgtatt tgccgttgcg gaaggatttt ttaattaacc
       61 acagataaac attgataacg attcgcttat tgtaatcaac agattaaatt tcatatgttc
      121 atatgtacgg atacattttc ggaccgatta attttaacca ttctatttat ttgcttacct
      181 attagatatc aataaaaaac tgcttaacag ctatttcagc tgttaagcag ttttaggatt
      241 tttagttcat attcattgtt tcttataata cagtgttcaa tacttctctt aaagcacttg
      301 ttgatttgct gaacattgct ttttcttctt cgttgattga taattcaaca acttcacgaa
      361 caccattttc gtcaacgatt gatggcacac ctgtaaagat atctttttcg tcgtattcac
      421 cagttaaata agcagataca ggcaatacag cttgttcgtt attcaagata gcttttacga
      481 tacgtgcagt actcatacca ataccatagt aagttgcttt tttacgatca atgatctcat
      541 aagcagcatt acggacttta tcagcaatca catctaattc atcttgggca atacgatggt
      601 ctttttctac aatttcaaac actggtttac caccgacagt tgtatgtgac catgcagcaa
      661 cttctgaatc accatgttca cctaagatat agccgtggac gctgcgtggg tcaactttta
      721 atttcaatgc gatttcttta cggaaacgag ttgtatccaa tgttgttcca gtaccgataa
      781 cacgtgatgt tggcagacca gattcgttcc aagcgatata tgtcaaaata tctactgggt
      841 ttgaagcaac aacgataata ccgtcgaatc ctgatcccat gatttctttt acgatttggc
      901 gcatgattga cgcatttgtt ttaactaaat ctaaacgaga ttgaccaggt ttttggttga
      961 taccagctgt gataacaacg atatcagcat ctttacattc ttcatagcca cctgaccata
     1021 cagccacatt ttcatcgccc caagccatac catctaaaag gtccaatgct tccccttctg
     1081 ctttttcttg attcacatcg atcaatacta attcattaga gattccttgg ttgatcattg
     1141 cataagcgat acttgttcca acaaaacctg taccgacgat tactacttta cgacttgttt
     1201 ttttcatgaa taaacagctc cttttcataa ctatttgtta ctttcatgaa aagtatagtc
     1261 ttgtttattc attttatcaa catgttaatt ataatagttg ttattataat ttttgatgga
     1321 aacgaattcc tgaatacgca taacagataa atgacaaatg tttacaccta tatcccctga
     1381 taatacaacc aaaagtttag aactgaaaat aaatccaaaa aaattcgctg caacgatata
     1441 ataagacagc aatcgcacct aaaacaattt tccttcatat gctttagtga aagattttct
     1501 tcttaatgtt tatttctcca ttcattggga gtacatgttg aatacttctt gaataatttg
     1561 aataaagaag tcgtttgttt atatccgata cgattagcga tttcttccaa cgaaaaactc
     1621 gtttcttgaa tcagttccaa cgcaaagtcg aattttttct ttagaaccag ttcttgaaag
     1681 gaagcatcaa aatacatttt caatttatta ctaagataga ccggattgta gccaaaatga
     1741 gctgctgttt cttttaacgt acaagaagta aaatgcttat cgatataatc taatactcta
     1801 agcatctcat tttctgcgca tccatctgct gatgaaaagc tttgttgttc taataaatga
     1861 gtcaattcga tcagtaatag tgatgtatag agatttaacg aacggatctt atgactgtat
     1921 gtatcaaaat aaatcaacag catattttca atcaaatgcg tcaaacgact tgtttttccg
     1981 ccgcgaaaat gaatgaattg tgtatgttcc cctttttctt ttgatgcatt tatcaaaaaa
     2041 gaagaaagta gattatttgt gcttgccaaa tgattcaata tctctgtaga taaggtttct
     2101 tcttttaata agatgttgat taaaatatcc tcttgccctg tgtattcgat tctttgagta
     2161 gcttctttat caatgatcac cacgtcactt tcttccatct ctatcctttc tccattaata
     2221 atttgtaaac atgtaccgct atacatataa ttgatttcaa caaaatcatg tgtatgctcg
     2281 ggaacttccg catatcgacg gtgtttactg atatgaatat ttccctgttg aaatagatcc
     2341 cctcggtgga tttttggcgg tgtctgaaga tggtaagcta agtttaaatc attgatattc
     2401 attccttgtt cacgctgcag agtttcgatt gattcttcgt tacgcaaaaa aaatgtctaa
     2461 ttcagctagt tccaattgac tccctccttt ttgtaagcgg tttctttcgc ttattagttc
     2521 acgctgtact gctattatat gcaaagctat ttttggtttc ataaagactt tctatggttg
     2581 ggacatgaac ttcctgtgat tccgctattg agagaataac cacttcatac tatatttttg
     2641 ataagcgctt acaaaaatga aaggaacatg cttatgaata aaaagatgat gaaattttct
     2701 ctgctgtcag tctctcttat tttgacttct gctggtgcag tcacgtctgc aattcccagt
     2761 atgtcagcta cttatgccac gcgttcttta tctgctgttg aattattaac gactgctccc
     2821 tcttttatgg tcactatttt tgttttgatt agtagtttta ttgctaggaa aatcggacaa
     2881 aaacaaactg tgatcatcgg attagtgatt gcatcgattt gtggtatcct ccccctcttc
     2941 tcacaaagtt acagtattgt cttagcttca agagctggac taggtattgg ctttggattg
     3001 atcaattctt tggcagtcag catgattgct gccttttatc acggagatga acgagcctct
     3061 atgattggtt ttcaaagtgc atttcaagga tttggtgcag ctttaatgac atttgttgca
     3121 ggacagctag tacagttcgg ttggcaatat acctttttag tctacgccat cagcttacct
     3181 attcttgtct tgtttgtttt gtttgttcct acacctgaaa caactgaact tcaatcgcat
     3241 ggcacagccc caacaagaca agtaatcaaa acgaatccta aaatgattag ttatggtatt
     3301 tttctcttgc tggtcatcgt ggtatataat gctgtatcga ttaaattagc cacagtcctg
     3361 acgacttatt ctctaggaaa cgaaactaat gcagcgaata tcttaacaat catgcaattg
     3421 gcaagtatgg ctgccggagt ttgttttggc tggcttcaga caaaagcaca tcgctatatg
     3481 cttacacttt ctttactact gatgggaatc ggctttgtat tgattgccac tgtgataaac
     3541 ctgtatgtga ttgggtttgg tgcgatccta acaggaattt ctttttcgtt attcattcct
     3601 tacttattca accaagtaag tatccaagcg cccaagtacg cggagagttt caatacttca
     3661 atcttattgg ttggcgctaa tttaggtact ttcattgcac catatggttt gctctttctc
     3721 tcttttgctt ctcttgggag taaaacactt gctgttttcg tcaatggcag tatcttgctg
     3781 ttgattagcg cgcttattag ccttctagta gtacacagga cgaatagaaa gacagtgata
     3841 agagtagatg aagaaataaa agaacaattg acaacaaaat aaatttgatg gaggtcgtgt
     3901 aaaatgacaa atgaaaaaca taaagataga tggctttggt atcctggaga ctttgagatc
     3961 cgccatggac tgctacaaaa tttccaacgg gaagaacgag gttttgactg gccagcgtac
     4021 tggtacatgg atgattgcca tcggaacgtc aagtttaaga gatattactt tcttgatcaa
     4081 ccttccatgt ttaaagtgac gatccaagga gttgggtatg tagaaattaa tggacaaaaa
     4141 catccatgtg gaaaatggct gacttgtcca gctggtaaag caaaaattcg tatctttgta
     4201 ggacatacat cagggctacc agcgatgttt atcgagggag acgaagtaaa atcagatatt
     4261 ggctggacag caagtaattt tattgaagaa tatccagctg gctggagtcc gctttatgtc
     4321 gatatcgcaa aagatccaaa tcaaatctat tatcaaaaag aatgcgtcca tccagtcact
     4381 gaaacggaga ttggcggagg aatcttattc gattttgggc gagcagtgaa tggctgtgta
     4441 gaactaatat ctctggatga ccatatcgaa tctatcactc tatgttatgg agagtccgat
     4501 gtagaagcta tggatatcga ttattgctac tataagcaaa taggaatgac aagaaaaatg
     4561 aaagctcgta aacgcgcttt tcgctatttg tttataccag aggtttctac tggaatggtt
     4621 caacttcaag tttttcatga atttttatct ctttcgaaac aagctgcttt ttcaagcgat
     4681 tcttctttac tgaatcagat ctggtcagta tcagaagaag ccttcaagtt atgtagtgat
     4741 ttgtttttta tcgacggtat caaacgagat cgctggattt ggtctggaga tgcgtatcaa
     4801 tcattcatga tcaatcaata tctttttttt gacgaggaaa tcaataaacg aacgatatta
     4861 gcattaagag gacaaaatga aatcaagcag catatgaata ctatcgttga ctattcgatc
     4921 ttgtggttga ttggtatcga aaaccactat atgatgacga aagactatga atttttacgc
     4981 atcgtctacc ctaagatgca atcattaatg agatacttga tggagcaaac gaatgaatta
     5041 gggtttattt atgggagagc aaaagattgg atatttattg attggtctga aatggataaa
     5101 gaaggaacgc ttgctgcaga acaagtctta ttactgaaag cctatcattc catgatcact
     5161 tgcggagata tcttagctga aacatatgga gagaacgaaa attatcatac tcatctctat
     5221 caagaaaaat atcaactatt gaaacaaaac ctatttgact acttttggga tgaagaactt
     5281 ggcgctttta tcgattgcta tgaatctggg agaagaaatg tgacgcgtca tgcgaatatt
     5341 ttcgctgttt tattcgattt tgctaacaaa gaccaacagc aatcgatctt aaagaatgtc
     5401 ttattgaatg acactatcac acagattact actccatatt tcaaattcta tgaacaagat
     5461 gctttatgca aactcggaca gacgaatatc gtctaccaaa cgattcttga ttattgggga
     5521 gggatgttat ctcatgatgc ggtaactttc tgggaagaat acgaccctac tcaaacagga
     5581 aatgaaaagt acagtatgta tggagatcct tatggaaaaa gcttatgcca tgcatgggga
     5641 gcaagcccaa tctatctttt aggacgctat tttatcggtt tgtatccaac aaagccaggt
     5701 tatgaagaat ttatgataca acctaatcta gattcatttg aaacactgaa ttgtacgcta
     5761 cctatcaaag atggaaagat ttcgcttgaa aaaacaaaag ataagatcgt gattacttct
     5821 gatagaacag gcggaacagt gaaaatgaat ggtaaaagtt atcctcttct agcaaaacag
     5881 ccacttgtct taacaagcta ttcaaaaata aatgcataaa acaaaaaact gcttgcgaca
     5941 tatcagaaga ctttaaatct tcttctgata ttgtccaagc aatttttatt ttgaagtttg
     6001 cttaattctg ttctttagaa acaatgtttg ttctttttcc ggcaaaaaga aagctagata
     6061 atacgacaag taaaaagaaa gcgatgattc caactgtcaa ggctatttga ccgtattctc
     6121 ctagtttccc aagtacaagt cctggtacta gataatcagt atcggcaaat gtagaagaag
     6181 cgattttaag atctcctaat actggaagga taaataatgg cagccaacta atcaataagc
     6241 tattgaaagc agaacctaat accgcccctc ttctaccgcc agtcgagttg ccgaagacac
     6301 cagaagcagc tccgcagaag aaatgtccta ctaccccagg aatgataact gtagtttgca
     6361 agcctatcat agcaaacatg gatagtgttc ctacaaagaa gctaacaaaa aagccaatta
     6421 atacagcatt aggtgcatat ggaaaaacga taggaacatc taatgctggt ttagaatcgg
     6481 gtacgagttt tttagcaatc ccttggaatg ctggaacgat ttctgccaac accattcgaa
     6541 ccccttgaag gacgataaca aacccagctg tgaatgttcc agcttggatc agtgcaaaga
     6601 ttacagcgtt gtctccattg cttagttctc tttcaacaaa tccaggacca gctaacaaag
     6661 caagtgcgac atatacgatc gacatcaaga gcatgatcga tacagtagaa tctcgcaaaa
     6721 agctgaaact tttaggaatc tttatgtcct ctgtagaacg gtctttgttt ccaaaaaact
     6781 ttccgatcca tccactagaa gcataaccga tatttccggt atgccctaac gcaacttgat
     6841 tgtttccagt gatgttcttc ataaacggtt gacaaagagc aggcgtaata gttaatagtg
     6901 ttccttcgaa gaccccgcct aataagatca tccacaaatt tgcagtaaac cccgcgctta
     6961 ctaaaatgac tgctgttaga caagaaacat aaagcattgc ttgacctgta agaaaaatga
     7021 atttatatct tgtgaatcta gcaagtaaaa tattcacgat catccctact agcatgatca
     7081 aagcagtagg tgtaccgtat tgttgcaaag caatcgccac tactgcttca ttgctaggaa
     7141 ctactccttg agtatgcaga gctgtttgga acatcgaagc aaaaggggtt agtgaattac
     7201 tgattaaacc agcaccgccg atcagtacta ggaatccgac aaatgttttg atagttcctt
     7261 ggatcacttc tgaaaatttc ttcttctgaa gaacgagacc aagcagtgca atcaaagaaa
     7321 cgaggatgga gggctgactg acgattgaca caaacaaatt caacaattta tccattgata
     7381 gttcctccct tgaatcattt ttattctttt gacaagatct tcgttacttt ttcttttact
     7441 tcttcgctgt caatgatcga ttgcaaaggt acgagctttt catcaggtat agaagttagc
     7501 gtatctttca aagaatgctc aatgaagaaa taatcagcca tatcgctaga tacacttcca
     7561 acatccgtat gacttacttc aaactcgtcc acatctgctc caagatctgc aagtgcttgt
     7621 ttgatgttca tctctaccat aaaacttgtt cctaatccac tttgacatac tgcaaaaatt
     7681 ttcatatcat tcgtctcctt ttcttatgat cgctaatatt tcttcttttg ttgatgcgtg
     7741 taataaatct tgaagatttt gtttattaga aagtagcttt gttaaatttg ccaaagcccg
     7801 aagatgtgcc tcattatcga tagcagctaa acagatgaac aaactaatgg gatgggctgg
     7861 attatcaagc agatccacag gttgttgaag tttcagcaaa gacatcccta cttcgtttac
     7921 gccatcttct ggacgagcat gaggtaatgc gatgtgatcg ccaatatgga taaacgcacc
     7981 atactgctcc acgcgctcga tcatagcttt gatataactt tcttctattt tattttctga
     8041 taatagcggc tgtgcggcaa atgtgattgc ttcttgccag ttttttggtt gatccgataa
     8101 ttggatcatt tcttctgtta ataattcaga tagcatagga cgttgatcct ccttcttttc
     8161 taaaagtttg ttcattttac gatttaatac ttgatagagc ttttcttcgt ctacgccttc
     8221 ttttaattcg atataaggca gtagcgccga caacaattct tgggcagaag gcattgaaaa
     8281 tcctggtatc agccaatctc tttgtacttg attgatcagc tgattttttt ccaagtcagt
     8341 catgattgga tgcagaacat agagagactt tttcttatca gtaagtgtga gaggtacagt
     8401 agaaaaaata atatcgtaag aagtttcgtc aacctcgtgt aagcggtcag ctgaattagt
     8461 ctctgtaaaa aagatcatcg ggaataattg ctgaagttca ctttttaaga tcaatgaaga
     8521 gctaatgcca cttggacaga cgatcacggc tcttattttt ctatttcttt cttctgcgtc
     8581 cgcttttctg atctctccgc caaatagtat agtgaaaaaa ccaatctctt cttcagggat
     8641 gctcttccct attcgtcttt ctaacggaag taatgctttt cttgtcattt cgtagatgga
     8701 agcatattga tgcttaattt gatcgatcat cacattaggt aaataaaaag aataggaaat
     8761 acgaaaatag gcagggacca agtgataaaa gacattcatc aataattcac gaaacgaagc
     8821 aaattctacg gccatcagga attccatgcg atggatgact tctgaagaac attccagcaa
     8881 aaaatcaagt gcaggctcct ggacatctcc ttcaacgatc gatgctaagc aaagagagaa
     8941 aaatataatc tccgaatttt gatgttcgga atagccaagg ccagtcaata cttgtcgact
     9001 aatgtcagtg actgcaagag aagccaacag ttcagcatct tttaccagtt ccatggatgt
     9061 ttttccaatt cgaatggttg tcaaactcaa ataataagct aattcatcca ttcgatttgg
     9121 tgcgaatcga agatctgata gtgaaatcgt tttatctagc aaatctctaa ctttggcgta
     9181 ataatcaatg gaaagggttg cggttaaata cgtgagccca aatcttccag caagtgactg
     9241 catcaaacga gcagtgcgat tttttgcttc tcttcttaat attgcttctg accctagcag
     9301 cctaaaacca gttttcctag tatacgacag ctgacaatct tccacttcta attcatttct
     9361 tatctttttg atatctgaca ggactgtccc tttagaaacc tgaagaaaat cttgaaaatg
     9421 aaaaacagaa acagacgttg cagtcgaata aaccatcaaa taaagaagat ctcttcgctc
     9481 ttgttcggta tacacagctt caaatcgagt aatatccaaa aatttttctg cccagtcgat
     9541 tgctcggaat ttctttatac ggatacgggt atcatccaca tattcggcaa aaggagttaa
     9601 ttgtttgatc gcctcgcttg tatttacttc gatgatctct gctgcttttt ctagactgat
     9661 ttctcttggt gttttcagga gataactaat tttgtagttc acagcgctgt tcacctccta
     9721 cttatattct agtatggtag cgcttttaaa tcgaggggtg tttggtacaa cttacagttg
     9781 gatttttttt acgactgtac cgaacgattt ctatcgaatg gatacagaaa aaaagcaggt
     9841 gaccaaatgt ctcctgcttt tatgaattga tttcgtccga tcaatgatga tcatgtttat
     9901 tttttgaggt cagttcttca aatttttctt tgacatcttc aaccgctttt tcgatatctt
     9961 tttcaaagtg attgggctca ttaggctttg tttcctcttc tacagaattt gaaccagata
    10021 aagctgtttc tttattttct gtttcaccag agtttgtgcc agctaatgat tcttctttgt
    10081 cttctgtttc accagaatta gttcctgcta aagccacttc ttcttctgtt ttaggggcca
    10141 ttgaacctct taatcgtttg tgtttttcac cgtcataatt tgtcatgttg gttcctccta
    10201 gtagttctat actttttcat cgtacaataa gacagtttat gcgtcaaaaa acaaacccga
    10261 ttcttacaaa tatgtcatat ttttccttct gattcttgaa tgcttttgat tccttcttcc
    10321 aaaattttca tttgtaagat tgtttcttca tctttgatga ctttttctgc gccattaact
    10381 aatgtctcgt aaatcccttc gtagacacgg ctgtaatcac caacctcgga aattactttt
    10441 tcttcatgat aactcccttg ttcatctata taggtcaata caccataatg ttcgggcaaa
    10501 tcgataccaa agtcttcatg atcaggcatg taaaacattt tcagatgttc ttcttgtcga
    10561 tcttttgttt gtttcacgaa catccctttt tttccgtaaa cgacgaaact tggacgttct
    10621 ttgatgcgga aataactgga ttttatcgaa attttcatat ttccatagaa aaaatccaga
    10681 tcgaagtaat cattcattcg tcctttccct aaaagttgac gtacatcata gctgatatca
    10741 tctggttcgc cgaaataaga aagaacttga tctactgtat gacaagcatg tccatataaa
    10801 tagctgttcg cagtagaaaa ttcatgcgta ttttctggta cttccggtct gaaataatca
    10861 taatgcattt ctacttctaa aatatcccct aattttccgc tttcgattac tttttggact
    10921 gtcaagaagt cagaatcgaa ccgtctgttt tggtaacatt ggataaatag acctttttct
    10981 tttgctaatg caaataactc ttctgcttcc tttgacgttt ctgcaaaagg tttctctact
    11041 agtacattct taccatgttc gagtgccatt tttccatatt cataatgaaa ttgactaggt
    11101 gtagtgatga caactaattg gatctcagga tcatcccaaa gatcagacag ctgatcagta
    11161 tattcgatgt cttcgaatgt tttccacact ggttcagatg gggaaaaaat ttttttgata
    11221 ttgattttgt tcgtaagttt tcttgaaaaa ggcaaatgat agcgatttgt actctttcca
    11281 ttgccaagat aagcgattgt aagcatgagt ggtgcctcct tttattgatg ttttcagtat
    11341 atcctagaaa agcgcagaaa gtatttataa tagctattct ggtaaaaaag ttcagaaaaa
    11401 cacttgttat cattcggttt ggtgaaatta atgttcaaag taaggataaa aatcaaaaag
    11461 atagtagcgg aaccaagtgt ttatcagtaa gatagaattt ggaggtcaga tcatgcttat
    11521 tgaagaaaaa ctaacaaaac aagagttgtt taccacgaca gagaaacgga ttgtcgatta
    11581 catccgtagg aatatcgaag ctgcagtcta catgacgatt gaagaattag caaaagctac
    11641 gtatacttcc cattcagcta tcattcgttt atgcaaaaaa atgggattca gcggatttaa
    11701 agaattccgt ttcgagctgg ggcgggaagt tcatcaattg attgctcagt tcaatcaaac
    11761 gattgatcct aattttcctt tttcaagtga agatagcact cagcaaattg caaagaaaat
    11821 ggcggattta tcgatccagt caataaaaaa agcacaattg cagattgaac atgaagattt
    11881 agatcaaatc ggtaaagtat tgaacaaggc acagcgaatc tttctttttg caaaaggtga
    11941 ttctcaaatc acggcaagga aattccagaa taaaatggtc aagttgaata agtttttgat
    12001 catggctgaa gaatacagtg attcttcttg gaatgcagcc aatttgacta gcgaggattg
    12061 cgccattttt attagctaca gcggtcgcat ccatcattat gaacgaataa tgacctattt
    12121 gaagcatgta gaggccccta ccttgctgat taccggaaat cagcattcag aaatggccaa
    12181 acaggctgat atgtgtcttg ttatatcgca ggaggaatat gattttgcta aagtagcgac
    12241 tttctcctct caaattgcct tcgattacgt attaaatacg ttattctcag tgatatattc
    12301 tcaaaatttt gaagagaata tccgcaactt gaaaaacaaa caagaattga tgcaaaaagg
    12361 ccttcttaaa gaatatcgga aaacttaaga aaagatcctc atttagtaat aagaaagata
    12421 gtgtttgata ggctctcttt gatatcatca taaaaacagg ctgaagtttt ctttttcaaa
    12481 cctcagcctg tttgtttacg gaacgattcg aatagatata ttatagaaat gaggaatagc
    12541 taacttctgt ttcttatttt gaacgattga cgaaaaagat atataagaag gaggaaaatc
    12601 agtatacatg cagctaacaa tccatacagg aaaaatcgtt tgttctcgga tactttctgt
    12661 acttcttttt gaacttcttt tgcatgataa ggaacccggt gccctctgac aagtaagcga
    12721 tgactgttga ccatataagg tgtacaagtt aggagtgtca cgaaatcctg ccctttttcg
    12781 attctcagat cttttgtatc agtcggttct accgtcttga tctgatcgac ttgataagcg
    12841 agtgtccgtc cattgatttc gatatagaaa gaatcaccta aagtcagttt cggtaagtct
    12901 gtgaacaact ttgcttgagg cagtcctcga tggctggaaa taacggcatg tgtattttct
    12961 ccccccgtag gataagacgt tccttccaat aatgaggcac ctttttctag cagcacagta
    13021 ttcgtttcat caaaaattgg caaatggaca ttgatctcag ggatcgtcaa aataccgatc
    13081 gtatgtttct caaaataggt tctatccttt ttcgcaggtt cttttttctt agtaaaggga
    13141 tctgcacctg gatttccacc ttcttttgct aattgctggt ttttctttgt catgttttct
    13201 tggattttag ccatgacttc ttcattttct ttgaccgctt gttgctgata atgagagatg
    13261 atctgctgat ccagataatt attcagcgcg tcgctaacaa atggataagc tagtatccct
    13321 attcctgaga gaagcaagaa aagcatcagc agatctagaa gtttcctttt ctttggtttc
    13381 atttgtgttc tccttgtgat gactgtttga taataaaaga aaaaggagca tttgcgtaat
    13441 ccgctgctcc tttttccaat tcttgcaaaa agactaaatc tgactgtgct tgcgtctagc
    13501 aaagtacagt ccagcaatca gtagaaggac tactcctgca ccgatataca catagattcc
    13561 cttaccgcct gttgaaggaa gtgtaccttt gtgtttattt ggtatttttt caggagaaat
    13621 caactgtcct gctgttacat aagattgttc atcgatagta aatgctacac ggtttgttaa
    13681 tggaacatat ttttctggcg ctttcgtttc ttccagatag tacgtgccat attttagacc
    13741 tgtcacatcg attaaaccgt tacttgtggt tgtaaaaacc gttgctgatt ctttcgccga
    13801 tacccagctg acggcttttg tggatgggtc gatcgataaa tatttcgctg tgtcactatc
    13861 ttgatcacga acgacgaatt ctgctccagc aagtgtttgg tctgatgtaa cgtcaccatc
    13921 tactttaaca aatcgtttgc ccccagtaac gacatcgact gacggtggtg tttgatcatt
    13981 tgtatgcccg ttatcgacat ttgcttggtt agaaaatcct ttggttggat ctgctttttc
    14041 gttcaaatgc atatagtaaa cgaatttcaa tgtaccgcca ggagttaatg aaggaatata
    14101 attcggatca actgaaaccg tgaatccgtc tgtttgctca gtgacagaat aatttactgg
    14161 gtcgatttct ttatttccat catataaggc ataagcatac gttcctgaag aatcattatc
    14221 aaatgttaaa gcagcgtcat gagtatcgat aagtttgaat gttgtgtact tgttttgcgt
    14281 tccttcttta tcagcgatac ctaatgggat attgacagaa atctcatatt gaattttttc
    14341 accgattgcg acatcttttc cattcaattg aggtgttgtt ttatcaactt tagacgtatc
    14401 atttttgatg gtcttttcta ctggtgtttg gctatttgcc aggatctcaa aaggcgtgat
    14461 tgtttgcttt tcaatcattt ccgcattatc aggagctttt acttcttcta aattatattt
    14521 tccaacttct aaatgattaa cgatcaactg gcctttttca atagatgcct cggcaaagtc
    14581 attgttgccg atgtcataag aatgaccagt aatgaaatgt ttggctttgg tttgatcagt
    14641 tgtccaagtg tacaatccat ctgtgacact ttggatgtat tttttgacgc ttggtgttcc
    14701 ttcttcttta gaaataataa attctgctcc atttagtgct tcgttttcgg cagtaccgat
    14761 ttttgtaact ttcaacgttc catcattacc gactgtattt ttagggtaga gatggatagt
    14821 atctagttct tctgtcccgt atttataaga gccatctgct tgtttgatca tctcatatac
    14881 agggaaagct aaaaccatgt ttgcggcagc tgacactccg tcttttggtt cttctttgat
    14941 cgtgtagact gcatctttcc cattttgttt tttaggtaaa gataaagtga catttccatc
    15001 agcatctgtc gttcctgaag caactggtgt accaggagtc aaagactgga ctgcttgttt
    15061 tgctgcatcc acggacgctc ctttatctcg ttgcgcataa aattcttgag tgacgttata
    15121 aactgaaaat gaaatatcgg ctaatccttg gtattgatcg aattcgctca tttctttccc
    15181 gctgttttgg attaaaggat cgggtaaatc agtcattttc tttttatgaa ggatgacttg
    15241 agctgctgtc tcctctgcaa acactttttt tgcgccgaag ctgtttgtga gtaatggtaa
    15301 aataaggaaa aggactccta acataacgtt tattttttta tgatttttca ttttttcact
    15361 cctgttcttt ttttgataaa caaccagatt gcaaagcttg cgaataccat accaacaacg
    15421 cctaaaagtg tatttttcgt ttcgtttgtt tttggaagaa atggacggct attttctttt
    15481 ttattgacta ctttcaatat actgtccgcc tctttatcct cagaaagaaa agttgaaatg
    15541 gtaaacggaa tttctgtttc acttaaaata taaccagaag gtgcttcgat ctcttttaat
    15601 cgataacttc ctgttttcaa cccggaaatt gaaaaagcgc catttttatc ggaaatcagg
    15661 tgaagcgcat ctgattcgtt ctttgtccac ttgtatccgt tggctgtttg ctggaggtaa
    15721 tttcctgctt tgtttttaat aaggaatact gcgccagcta atcgtttatc tggtttattt
    15781 gtctcgattt tgacaaattg cttactccct gtatatacag agactttctt tgtactgaca
    15841 ccaaaaccaa aatccagtgt tccgttgttg ttgatttcct tgttcgcctg tgctgtactg
    15901 cttagctgca tctgataaga aatggtcagt ttttttccag catatttttc caacgaaggg
    15961 atcgaaaata aaacatcaaa tccgtgaggt ttcttgtgta tgacaaagcc ttctgtcagc
    16021 ttttctccat tcgatgaaat cgttaaacta ttaggtaaaa acgttaatgc ttcatccgca
    16081 ctatctgaca attcgaactt ttgatagtca aggatattta caggaattgt cgtatgcaag
    16141 gaataagtga ttttttcacc aatcgtaaaa tcatttctag gctcttctaa taccttctca
    16201 aaaggtggat cagggtagtc gttttcttca tttttaggat acaaatggat agttgaaagt
    16261 ttttgattgt tttgtccatg tacaggaaga acaactacca tattctctgc cttttctttg
    16321 acgacttctg gtactttgga ttcaatgaaa agatacgctt tatctctttt ttcttgatct
    16381 ttagcggcca gagaaaaaga agcgatacca tcttcgttgt taagagttgt agttgttgct
    16441 tctgcggtaa acataccgga agacgcaccg ttttttgcga tctctgcttg tgcttcttct
    16501 accgttttgc ccttttcccg tagatggtaa aaagaatctg tgacatcata aacttggaat
    16561 gtgacaccat ttaatcctcg atacgtttgt aataaagctt tttcttgtcc gtcatttgga
    16621 tgatttttcg gcagttgccc gtttgggaaa agcaatttgt gtaaagtaat ctgaaccatt
    16681 tctgtttctg tttctgttgc tacctgagta aaagctggtt taaatagtaa caagaagaga
    16741 cacatactaa gccaaccaag ttttttcata tttagctgcc ccctctttct tttttcaaaa
    16801 aaagatacca aatagaaaac gcacaaccaa tcatccctac tagatagatt cctaaacggc
    16861 caattcctcc cgtttcaggt aatggtactt ttgcctgatt cgtgatgtct aaagaaatct
    16921 ggttgttttt ggctcctggt gacagaacag attcatgatc ttgtccatcc acttgaattg
    16981 acccatcttc gtgtataact atagtaactg gctcttttag accttggtat ccttctggtg
    17041 taaaagtttc ggtcagcgta taagttccag gttttaaatt ttcgaataga aaggtatctg
    17101 tttcttgtcc atcttttggc gattctaagt ccatttctgg tccctgcagt ctgaattttg
    17161 ctccttttaa tgtctgtccg ttgtcttctt ttttgtgtac cgttagatca aacgctttta
    17221 gtcgattttg atgagtgagc gtccatacat tatttgtcat ctcggtattt tttcccgtgt
    17281 aaatgatttt tccatattta tcgatttgga attcataatt tcccggcgaa gtatcatatc
    17341 cggcaggacc agctgtttca acgactcgat atttaccagg tgtgagtgaa tcaaaactgg
    17401 caagaccttt ttcattcgtt gtttggctgt caattggctg ataagtacca tttgcttctt
    17461 ttttctgtag ggaaaatgct gctcctttaa gatttatctc tttgtccgtc ccttgcatcg
    17521 catacttacg tactgctaca ggaacttcaa caaaaggatt ttcaatagtc aactggatcg
    17581 tatcatcgga agtagtgact gtttttccat caatggttac cgtaccatca gaagcgatct
    17641 tgatttccca agtagtcttt ttgcttaatc catgcccttc tggagctttt gtttctgtca
    17701 gcgtataggt catttctttt tgcaatttga cattttctgg aagagaatag gttccgtcgc
    17761 catgatctgt tagtaaagta tcaatagaat ctcctgttaa tttgaaaaca gcgccaataa
    17821 gatccttttc acctgtagag gaatttttcg ttattttcaa gtttaacggt gtgaattgtt
    17881 tagtattttt ccatgtcatt gcatctattt gttgagaatc gtatcctggt acaggtaatt
    17941 ctttgattgt ttggtaactg aatgcttgac cttgattatt gtattgaggt agtgataata
    18001 tctcttgata actttctccg ctatttgcag ataatttgtc aatgtctgca cgttcccacg
    18061 tatttgttgt atctttagct ggtttaatga ttcgaatata tccgtttttc caagctgcag
    18121 catttgtcgt atgttcccgt tgaatctcaa aagtaacttg atctggacga tcagctagat
    18181 tgttgtcaaa ttcttcccat aactttttga tgtgaagact gactccggga gctttagcag
    18241 atggtatacc gaattcagct aattcattgg tatcaatgtt tggctggaaa gttgtcctgc
    18301 cgttcatttg ataccagaaa tttggatgga agtcctcatt ttctgtttgg attctcactt
    18361 ggtaatggat ttggatttct tggtcttttc ctaaatagat ctgattgctc ttgatggtat
    18421 ttccttctat ggaaatagat ggagtgaccg ttgtaggact tgtccccaca ctcttgactg
    18481 aaagtgtacc aggctgataa atgaatggtt ctgcgattgg atcatttatt tgtccattgc
    18541 ttacagttgc tgagatctgt acagcttttt tggctaggta ttcagagata tctgttgcat
    18601 gatcagctga ttcatagtaa agatcgcctt tttcatctga tgaaaccatt tgtcgcatac
    18661 gagactctac ttctgctttt gagagaccag ctgccggatc gctttgaagt tggatgccaa
    18721 gaccatgtat ttcgattcct cgttctttga gtgccatcgc ttctccgatg gttgcgataa
    18781 acgtactgtc gattcttctg gatagattgt tttggtcagg tgcatcatag attcttgaga
    18841 ttagagaagt atttcccggc cgatcttgcg tattagaaaa ctgagttccg taataattgc
    18901 tgcttgattg tgcgtgtacc cgctgtactt tataggaaaa tgttggtaca ccatccgtca
    18961 gcaaaacgat cactttttta tgtccatttg gaacggatag catgcttcct gcatctctta
    19021 gtgctttttg tgtaaaagta ccaccatttg tccgtgaagg tgtaatggat tttacttgat
    19081 ttttcactga atcaaatgaa cccatctgta ctgcaccgtt actgtagcta tatccttcgc
    19141 ttgagtagcc gacatatccc atattgattt tgtctgtgat accgctatct gctaaagtat
    19201 cgacaaaacg atcgacacca atctttactt caccgatccg attattgtcg ttcatacttc
    19261 ctgaccagtc tacgaccaat acgagatcaa gaggcgtgat atctttttgt acatttccac
    19321 gagcattcaa ataaacatca aacaatccgg gtgtacttgt ttcctttgca tactttcgta
    19381 tcgcatagtc tgcttgatta ccggttcctc catattcgat atacgaatgg gtccgatcat
    19441 taggatctcc attccaagaa tttataccat cccattgatt tgttccgttt ttattgcctt
    19501 gatgatcaag aacatttgta tttcctgtag gttgccaact ggctttagga taagtgccct
    19561 gatcatctgt tgtatattct gtaggaatcg tttgattaga tggcacactc acagctggca
    19621 aggataatgt aggcaatgta aatgggctga cagaattttc gttttctttt gatgattctt
    19681 cttctatttc gtctggcttt ggtactgaca catctgtatc tttcttattt tctgtctcat
    19741 caataatgtc atattcttta ttatcgacga tcagcttgtt tgcctggctg tggtaaagtg
    19801 ctggatcgat tggaaaactc agatcgatag tttgtttagt gtttgatgag atttccaacg
    19861 taattttccc gctatttacc tgataagcat agccatctcc ttcttttctt tccacatcac
    19921 ttaagtaatt ttcgactaga ccaatttctt tttctatctt ttcagcactg gtgttattcg
    19981 catttagctt gatgttcagt gctttttttc cagactgatc cgaaatgact tcagctgaaa
    20041 ttgtttctaa aactgttgga tcatctgtag tgtctgctat tgcagataat ggcacaaagt
    20101 ttgataaact caatattatc agcatgaaaa taactttcag tttcttccct gtggtcgtca
    20161 tttttcccct cctttttctt tttttctgtt ttgacgtgaa tagtgaaaat tattaaaata
    20221 aacaggcgtt acataatttt atttatttta atatgataat tattatacga agggcgctaa
    20281 agggaatcac accaattttc ataccaattg gtttcattta tgaagttgat tccttcgatt
    20341 aatgagcgtt tcattgaaag aaaagggttg agggagaaaa acatatgatc aggttgttgg
    20401 aaggaaacta tagaaagatg gaaaaaatca ttcgaattct gtccatttct gacaagtgga
    20461 tgactaaaaa agaacttgcc aaacgaattg gtagttcaga atctacattt gtaaggtata
    20521 tcgaagagat caaaatccgc tggggaacgg atgtaacgat tcaatcttca caaaaattgg
    20581 ggtatcgtct tgaacatttg aatgtaccag cttatttaaa tgttctgatg gaaatgtcgc
    20641 aaagttctac aaacagtcgt ttattaaatg agattattgt caatccgggt aaaacgattg
    20701 agtattattg tgaaaaaatt tccatcagcc ggtcttcttt tacacgtaag ttgaaacaat
    20761 gcaatcaaat tttagaaagc tatttattaa aagtgatcgt tgaccaaggt tttcagttaa
    20821 ctagccttaa aagtgagctc cagttgcgta tctttgttac ctttttcttt ttaagttact
    20881 acggaagacg cacgttgcca tttccaatcg aaaaaaagaa gatcaaagaa attatggtca
    20941 agaataattg tcagatgaat ttgtttcagg aagaaagccc atatgagcaa tctttcttta
    21001 ttatgtattt tgttgtgcat ctcatgagag aatcccaagg aactccattt gaatggaaag
    21061 ctggagactc ttatgtacag ttgataaata tcgataaaaa agacttctta atcttgaaaa
    21121 atctcttagg aaaattgaag tacagcagct atataaaagt agtgaactat tttgtcaata
    21181 cgattttttc agaattttcg gagaaagaaa aaaatgaaat cagtgagcag atacgaaaaa
    21241 atatgtccta ctgttcgttt tcatccgctg attgcaaaga aaaaaacgaa gaatatgttg
    21301 ttcaattatt aacgcaaatt tattttttct cccgtatttt cccatacgat acgactcatt
    21361 tgacgatccg tgcaacttac tttgcacagc agatgcaaca ccgccagata cgtctatatg
    21421 ctggatttcg tgcagaactt caattttatt ccactctact gaatcagaat ctgatttatt
    21481 atttatctag ctatatcttt tggatgctta tcggtaatcc tgaaattcac cattatactt
    21541 ctgataaaaa aatactgatt atcagcgact taagtcttcg acatagtcaa tatattgaag
    21601 aatatatcag tgatattctt gctgtgcata aaattcactc tgagacaact gctattactg
    21661 aagatcagct gtcgaaatac aatcttctcg agtatgatct gatcgttacg aatcaaccca
    21721 ttttgaatgc ccatgttcct catatattga ttgatgatag tgtgtcattc gcaaatgaag
    21781 aagaactcat ccgcttattc gagttatgaa taatcaaaaa acagctatgt ataaaccacc
    21841 ctgataaaaa tttcaggaca aatacacata gctgtttctt tttattgtat tccttcagca
    21901 atccaacgag atttatagcg aaagtctttt aacaactctt cttttgtcca attatttgca
    21961 atgagatccg tcaaagtgtt cattacgagg gtcatggaaa agtataggaa actaactgct
    22021 tcttccgacg attgaatgga gaaatgattg attcttaata attccagcca tttttcgttc
    22081 attggagaat cagccgatag ttctctacgt ttggttgtct gctgatcatt tcgctgggtt
    22141 aacaagtata acctttgcca atagatactt tcgtgatcta agtcgcagca ccactctaaa
    22201 tatttttcga tccccaaaaa gaaatgttgt ttgttttcgc taatgaattt taagatatct
    22261 tgatggacta tgtgagaact gcttttgatc gtatacaaat aagcatcttc caaatcttga
    22321 aaatatttat aaaaagcacc gcgcgacatc cccatcttgt caacgatttc tgagactttg
    22381 acttggctga tgtgctgtga gcaaaatatc tccagtaata ggtcatcgat tctctgtttc
    22441 tttgcgttcg ataaattgaa aaaagtttct tttggcatcc acatctctcc tttatctctg
    22501 attatacgtg gatagccctt cattgtcatg aaggattcat tccttctttt tctcttcttc
    22561 tgtcaagtac ccatcttcca ttcgataaac gcgatcactc cattgaatca tacggacatc
    22621 atgagtaacc ataatagtcg ctttttgttt ctcatgagct tctttaacta gtaatttgac
    22681 gacttcatat gcatgatctg tatccaaact agctgttggt tcgtctgcta agatcaagct
    22741 tggctcatta tataaagcgc gagcaattgc cactctctgt ctttctccac cagaaaggtc
    22801 tttaggataa ctatttttaa gtgactggat atctaaagat tgcagcaagg aatcgatttt
    22861 tgttgaatct tctttttttg aagtaacttg gtcaactagt tcaaattgtt cctttaccgt
    22921 taaaaaagga atcaaattag agctttggag aataaatcct atttctttaa agcgtaaatc
    22981 tgctcgtttt ttttctggaa gctcagttag tgagtgaccg ttgatcacta tttctccgga
    23041 agtcggtgtt tgcaatcctc ctgaaatggt aaggaaagta cttttacctg aaccagaagg
    23101 accaatgatg ctgacgaatt ctccttcctt tacataaaaa tcaattgctt tcaaagctga
    23161 gacctttgta tgaccagaac caaatgtttt ggtaactttt ttcatcgtta atattttttt
    23221 catttttatt aacctcctat tgctgtaatt gggtctactt tggttatcgt acgtatcgaa
    23281 aacaatccgc ctagcattgc gacaatgatc aaaatcccac tatatagcaa ccattgtgac
    23341 caaaaaacag caaaaggcat cgcgcttggt aaaatcagac tagtcaaata agcaaacaaa
    23401 agagcaagca gaacgcctac caccccaaca ataaaggact gtgctactaa agatttagcg
    23461 atgaagaaat ttttgatccc ttgtgctttc attactccaa aaatagcagt cttctgaagt
    23521 gtcatcacgt acatgaaaat accgaccacg gcagcaacca ctaagaacaa aaagtatatc
    23581 atcgcattta aggtcatatt ttgggcagaa tagccaggaa tactttcaat gaatgtccca
    23641 atagttaata actgtagacc cttcgatgct ttttctttcg aaattttagc tgaatccttt
    23701 attacgatcc cattgatcgg ctgttcttct tcagaagcaa acggctgttg tccatacttc
    23761 aaagcagtcc acgtatctag ggacgcatag atgacgggag aaactgtgta ataagtttca
    23821 ggaaagatac cgacaatcgt caaaggatcg tcgtaactgc caattttgat cgtatctcca
    23881 attttatagc ctgaatcagc cagattttga gagatgatga tttcattctt tgttttaaat
    23941 tcatgccctt tcgtgagttt tggtaataaa aatgcttctt tcgttgtgcc gaaaactgta
    24001 acattttctt tgttttttcc tttgattgct ccgctgtaca atccgattgg tgctttctct
    24061 cctccttcaa tgtatttcag atctcctctt gttaattgtg aagctgcaaa tacttgattt
    24121 gcatcctccg acaaaacaat ctcttgtgcg tcccaatcat cgattgcttt tttaaactct
    24181 tctgaaagtc cattcgctag tcctgacaac atgaatacga catacgcaat caagaacata
    24241 ataccgacga tcaagccata tcgtaattta gaatagcgaa tttctttgat agctagaaac
    24301 atttgttttt cctccctatg atagattgat agtgacaaaa tgtcacttgt tgaatagtgt
    24361 aactctaaag tgacaatttg tcacttgtgt tgtttttttc ttatcaataa attgtctaag
    24421 acatctttcg ataaaacctt aacatgagtc ataaaaaatt tatttaagcc ttatttatgc
    24481 tttccatgat aaactaaagt gaccaaatca atgaggtggg aaaatgatta aactattagt
    24541 agtagaagac gataaagtat tatccaataa tataaaagaa atactcgctg caatcggcga
    24601 gataacacaa gtttatgatg gtagtgaagg actgttcgag ttggaaagtg gagtttatga
    24661 tcttgcaatc ttggatttga tgatgccgga aatgaatgga tatgaagtgt tagcaaaaat
    24721 acggaaagaa aaaatacaga ctccagtatt gattttgact gcaaaagata gtttggaaga
    24781 taagattgaa ggatttgaac gaggtgcaga tgattatcta acaaaacctt tctatcgaga
    24841 agaactgatc atgcgcgtga aagcattatt gaaacgctcg ctaggtctct ttgatgaaaa
    24901 tgttctgaag tataaggata tcagttgtga tttaaaaagc aatgaagtct tgtataaaga
    24961 acaagttttg cccatacaag gaaaagagtt tgaattgctc gtttattttt tacaaaataa
    25021 gggaatcatt ttgacaaaag agcagatatt tgaccggatc tggggatttg attctgatac
    25081 gacgattaca gttattgaag tttatatgag caacttacga aaacatctgc gaagtactgg
    25141 attggataaa gaaatcaaaa ccttacgtaa tgtcggttat atactgcaaa gggtgtgatg
    25201 caatgaatgg agatattaca cgaaagcaac gtagtcgctt ttttgcgaca aatgtagcag
    25261 catttgcttt tatctttttt cttttgggat tcattacttt acagatatta aaccaatctg
    25321 catacagaga aacagatctg tcattagaga aaataaattt tgattctcga acagtacaat
    25381 tagagataca aagatatcag caaggcgatc cctttttaaa cccgactcaa acggaacaaa
    25441 acagaacaaa catggaacag ccattaatgg aaccatcaaa tgaccgtttt aacactcaag
    25501 tgatcttgtg gtctaaaaat ggtgagatat tgaataaagc agctattggt gggcgtttca
    25561 accaaatcaa agaattaaaa atggatacga aaaatctgaa taccatccaa aatattgaat
    25621 taaatgaaga cagtggctca tctgccctct ctttccgttc gattacgaaa aaagcggaga
    25681 cgaatcaaga tggcgttgcc tacatccaaa ttttagaaaa tacaaatcaa atcaagaatt
    25741 cattagcgac ttttcgcacg attatgattt tgtgtatgat tgttttttgg attctctcta
    25801 ttggtatcag ttattacttg tctagtctaa gcatgcgccc tattattctt tcttggagaa
    25861 gacaaaaaga atttgttgag aatgcttctc atgaattgcg cacaccgtta acgattattc
    25921 aaaatagttt ggaacatctt tttaccaagc ctaatcatac tattattgaa gaatccgaaa
    25981 gcatcgctca agcattgtca gagacgaggc gcctcactgg attgacttcg gatttgctga
    26041 caatcgctcg gaatgattct gatcagcagc tgctttcaaa gcaaatgatc aatacacaag
    26101 aatatatcga acaacttgtt aaaccatttc aagagatggc catcatagat ggaaaagagt
    26161 ttattctcca taacgatgca acattagaag taccaattga tgaaaaaaaa atccatcaag
    26221 ttttggttat tttgctggat aatgcattaa aatatacgaa tccagaagat aagatcattt
    26281 tggattcatc tgttaaccat aaagaatggc tgattcacgt aaagaatact ggcccttcta
    26341 tcagtgaaga agatcaagca aggatttttg atcgatttta tcgggaagat caatctcggg
    26401 cgaaagagac tggcggatac gggcttggct tggcaattgc aaaacaggta atcgaacagc
    26461 atcatggaca gatttctgta tccaattatc ttccacaagg agtcgatttt aaagtccaat
    26521 tgccgttgaa gcctaaaaaa taatgcttat ccctaaaaaa gggctgtgac aaaaatcttg
    26581 tcacagccct tttttgttgc gaataaacgg tgttccagaa gtagcttctt cggaaataag
    26641 ccgaaatttc tttaagctga ccacttctgt cacaacctct ttagcttacg cttacgattt
    26701 tttattgcgg tcttcaatgt gtacatcacc gcttgtgtca atatccaatt cttctcggct
    26761 aacatcttct gtcacttttt caacatcttc atgttcttct ttatgaatcc caacttcttc
    26821 acgaataact gtatgtttgc ttacatctac ttgctcttct ttgattggga tagtaatcgt
    26881 gtcatcttcc aatttttcgt ctgttccctg tgaagattga tctgttactg gtttgcgttc
    26941 gatgacgatt tcttcacgtt tgaccggtac ttctaccgtt tcagtatcgt tgaccacatg
    27001 tttgtggatg tcgacttctc cagttgtcac atcatgtgta tttacatcta atttttcttc
    27061 ttttagttga attgtatcat catctagatt tggtttgcga tcgatgtctt cagtcttgct
    27121 tgtacccatt gtatcagtct cgccaggtac gtccatcgga tcagttcctg gtgtcatatt
    27181 caatggatcc gttgtatttt gaaagcctgt cgtgccacct acgtccattg gatctgttcc
    27241 agtgccagtt tctaatccag tccccattgt gcttgctggg ctaacagtat cttgtgtatc
    27301 cattgtatct tctgttgttt ctggctgacg atagcctttt actgtgatga caaattttcc
    27361 atcagaaata tctcctctat attgatataa aggatcattt tctggtgtag ccgtctcaga
    27421 atcataggta tcaaaagaaa aagtatcttt tattttttcc cataaagaac ggtcttcttc
    27481 accatccatc cgattttcat ctgtttcaac atctatacca gacaatccgt caaatcctag
    27541 tctagatgct gcttcatcag tagtataaat aacgatatca tctcgttcat acccttcgtt
    27601 acgcaaccgt tcaattactg ataatgcttc ttccctcgta ctatagcttc cgatgaccgt
    27661 taaatttttc tcttccatgt aaattcctcc tagcaaatag atttatgatt ggtatattgt
    27721 tacttataaa tttattctat aggagacgat tgttttctac aaatatttag attggaattt
    27781 gattaaaaca atttaattga aatttga
//
LOCUS       DADVXU010000028        27774 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.030, whole genome shotgun sequence.
ACCESSION   DADVXU010000028 DADVXU010000000
VERSION     DADVXU010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 27774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.030"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(338..2230)
                     /locus_tag="KB953_001133"
     CDS             complement(338..2230)
                     /locus_tag="KB953_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6866121.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2227..3954)
                     /locus_tag="KB953_001134"
     CDS             complement(2227..3954)
                     /locus_tag="KB953_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6866122.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3970..4620)
                     /locus_tag="KB953_001135"
     CDS             complement(3970..4620)
                     /locus_tag="KB953_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBB6866123.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4824..5120
                     /locus_tag="KB953_001136"
     CDS             4824..5120
                     /locus_tag="KB953_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBB6866124.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5312..5932
                     /locus_tag="KB953_001137"
     CDS             5312..5932
                     /locus_tag="KB953_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HBB6866125.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(6024..7190)
                     /locus_tag="KB953_001138"
     CDS             complement(6024..7190)
                     /locus_tag="KB953_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBB6866126.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7202..7555)
                     /locus_tag="KB953_001139"
     CDS             complement(7202..7555)
                     /locus_tag="KB953_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBB6866127.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7683..8705
                     /locus_tag="KB953_001140"
     CDS             7683..8705
                     /locus_tag="KB953_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HBB6866128.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8724..8885
                     /locus_tag="KB953_001141"
     CDS             8724..8885
                     /locus_tag="KB953_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866129.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9144..9578)
                     /locus_tag="KB953_001142"
     CDS             complement(9144..9578)
                     /locus_tag="KB953_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866130.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9688..11547)
                     /locus_tag="KB953_001143"
     CDS             complement(9688..11547)
                     /locus_tag="KB953_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBB6866131.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11665..12825)
                     /locus_tag="KB953_001144"
     CDS             complement(11665..12825)
                     /locus_tag="KB953_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HBB6866132.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(13132..14556)
                     /gene="araA"
                     /locus_tag="KB953_001145"
     CDS             complement(13132..14556)
                     /gene="araA"
                     /locus_tag="KB953_001145"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HBB6866133.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14572..15270)
                     /locus_tag="KB953_001146"
     CDS             complement(14572..15270)
                     /locus_tag="KB953_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBB6866134.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15227..16888)
                     /locus_tag="KB953_001147"
     CDS             complement(15227..16888)
                     /locus_tag="KB953_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HBB6866135.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            17106..18506
                     /locus_tag="KB953_001148"
     CDS             17106..18506
                     /locus_tag="KB953_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HBB6866136.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18758..19837)
                     /locus_tag="KB953_001149"
     CDS             complement(18758..19837)
                     /locus_tag="KB953_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBB6866137.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19923..20882)
                     /locus_tag="KB953_001150"
     CDS             complement(19923..20882)
                     /locus_tag="KB953_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBB6866138.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSKKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20894..22405)
                     /locus_tag="KB953_001151"
     CDS             complement(20894..22405)
                     /locus_tag="KB953_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HBB6866139.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22427..22990)
                     /locus_tag="KB953_001152"
     CDS             complement(22427..22990)
                     /locus_tag="KB953_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HBB6866140.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22996..23859)
                     /locus_tag="KB953_001153"
     CDS             complement(22996..23859)
                     /locus_tag="KB953_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBB6866141.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23873..24769)
                     /locus_tag="KB953_001154"
     CDS             complement(23873..24769)
                     /locus_tag="KB953_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBB6866142.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24944..26233)
                     /locus_tag="KB953_001155"
     CDS             complement(24944..26233)
                     /locus_tag="KB953_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBB6866143.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26205..27722)
                     /locus_tag="KB953_001156"
     CDS             complement(26205..27722)
                     /locus_tag="KB953_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HBB6866144.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
BASE COUNT         8105 a         5857 c         4818 g         8994 t
ORIGIN      
        1 aaactggctt ttgaacaccg tttaccaaga ggttgtgagc ctgccgttta actccgagtc
       61 acaaggagca atttcaaaaa acagcttctc aggtttgttg actattgtga cttgtgaccg
      121 aggagttggc aggagaacac cgtttaagga agaggttgta aaatcagcgg tttgacctga
      181 gtattagaga tacaaaacgg aaaaatagct tctcatattt tgccgttttt tgagctaata
      241 ccgaaggaca gctgattgaa caccgtttat tcggaaaaag ggactgtgac acgacttttg
      301 tcacagcccc taaaaaaaat aataaatttg tactattcta tgaagcttct tcttcagaaa
      361 actgactgtt ataaagatct gcatagaatc cgtctttttc catcaatgta tcgtggtttc
      421 ctgtttctac gatcgaacca tgattcatca caataatgtt gtctgcatcg cgtattgtag
      481 acaagcggtg agcaacaacg aaacttgtcc ggttttccaa taggcggttc atcgcttttt
      541 gaatcagtat ttctgttcgt gtatctacac tagatgttgc ttcatcaagg atcaggacat
      601 ctggatttgc taagaatgcc cgcgcaattg tgattagctg acgctgccct tgcgaaatat
      661 tgcttgcatc ctcgtttaat acagtgttat atccttctgg caatttacga acaaaatcgt
      721 ctacgtgagc agctttagca gcctcaatga tttgttcgtc tgttgcatcg tcgttcccat
      781 atttgatatt gtcatagata cttcctgtaa acagccaagt atcttgcagt accattgaaa
      841 actgtgcgcg caattcatca cgtgaaagat cacgggtatc tacaccatcg taacgaatac
      901 tacctccaga aacatcgtag aaccgttcaa gaaggttgat cagtgtcgtc ttccctgcac
      961 cagtcggacc tacaatagcg accatctcac caggtttgac atccaagtta aaatcagtca
     1021 ttagcaagtt atctggtgtg tagccgaatt gaacattttc aaaacgaact ttgtatggtg
     1081 tatctttttc ttctggaagg ccagattttt cattggacat ctcttcttca tccaacactt
     1141 caaatacccg ttcggctgaa gcaactgttg attggatagt gttcatcaaa cttgcaagtt
     1201 gagtgattgg ttgagagaac tgtgttgtat attgcaagaa cgcttgaaca tcccctaatg
     1261 tcatgttccc atttgcaact tttactccgc ctagtacagc gacgaacaca tagcctagat
     1321 ttttaacgaa attcatcaac ggcatgatga ttgccgaaat aaattgagct ttccaaccag
     1381 cggcatacag attctgattt tctttttcaa atacttcctg gtctttttca gtatgattga
     1441 atgttttaac aatggtatga ccaccatatg tttcttctac ttggttattc aataaaccta
     1501 aacttttttg ctgatctgcg aaaaacactt gtgatttcgg cgctacgatc atcacgatga
     1561 tcaagcttaa agggatcatc aataacgcga ctaacgtcaa ttgccagctg attgaaagca
     1621 tcatcacgat gaccccaatc aatgtgactg cactagttac aaattgagtc aaattttgct
     1681 gcagcgtgct tgcgatgtta tccatatcat tgattgctcg agacatgata tccccatttg
     1741 tatgtgtatc ataataggaa atcggtacac gattcatttt ggcttccaaa tcatgacgta
     1801 attcataaac tgttcgttgg gaaacacgtg tcataataaa ttgctgtaaa aagttgaata
     1861 cagcagaaat caaatacatc acgattactg tcgcaatgat ctgcgcgatt ttatcaaagt
     1921 ctatcggaaa ttttgttact tgttggcctt gttgcatctg ttgagcgcca gccattacac
     1981 ctttgaatat ttctgtggtt gctttcccca agacctttgg tgtttggatt tggaagatca
     2041 cggctgctat tgctagtaca aatacagcga tgatcgcaac catacgttta gacatatagc
     2101 caaacaatcg gcgtaccgtt ccccagaaat ttttaggctt ttcacctttt acaccgagat
     2161 ttctgccagg tccaccatgt cctggaccgc ctgctggtcc atgagaggga cgtgtgtttg
     2221 ttttactcat gctaagtcct cctctcttag ttgtgaatga acgatttctt gataagtttc
     2281 gtttgttgct aatagttctt catgtgttcc tttgccaacc agttttcctt catctagcac
     2341 aaggatcata tctgcatcca ttactgtact tactcgttga gcaactaaaa cggtgatact
     2401 ttctttcata ttcgttttca atgcttggcg aagattggca tccgttttga aatccaacgc
     2461 tgagaaggaa tcatcaaaca catagatatc tgctgttttg actaatgccc gtgcaattgc
     2521 cagacgctgt ctttgtccac cagagaagtt tcctcctcct tgttccacat ggctatctaa
     2581 gccatcttcc atctctgaaa caaaatcttt agcttgtgcg atttctaatg ctttccaaat
     2641 catttcatct gttgcatccg gcttaccata ctgcatattg tctcggatcg ttccagagaa
     2701 cagaactgct ttttgaggaa caaagcccat taattctcgc aaattatatt gagtcatgtc
     2761 actaatgtcc gttccattta tttggatcga accagattcg atgtcatata atcgagggat
     2821 caaattgact aaggtcgttt tacctgaacc agtcccccct atgattgcga cgatctcgcc
     2881 cgacttggct tggaaatcaa tatcttccaa tgcaagtttt tctgctccat gatatcgata
     2941 attgacatga ttgaaggcca atgttgcgtt ttttccagca aaagaaactg ttttaggatt
     3001 ttcagggtca tggataccga ttttttcatc caacacttca ttaatacggt cagctgaagc
     3061 ttgtgcacga ggcaccatga taaaaatagc ggaaagcatc ataaagctga tcaagatctg
     3121 catcgcataa gtcataaatg caatcagatt accgacttcc aaagtcatat ctgctatgta
     3181 gtgtccacca aaccaagtga ttgctacgtt tgtaccgctc atgatcaatg tcatcattgg
     3241 catcatcaat gcgacgatcg tattaacttt gattgccgta tttgtataat ctttattcgc
     3301 taaatcaaaa cgattttctt caaagcgtgt cttatcgaat gctcgaatca ctctgacccc
     3361 tgtcaatcct tctcgaaata ccaggtttaa acggtcagtt ttcttttgca tacttttgaa
     3421 taatggaaca gcaaagtaca tgattccgcc gatcaaaata atcattactg ggatgacaac
     3481 taagaagatt tttgtcaact gatgatcttt ttgataagca agtatgctcg cgccaatcaa
     3541 cgtgattggt gcattgatca tcatacgtaa aaacatttgt gtaaccattt ggatttgagt
     3601 cacatcattg gtcgttcttg tgatcaaaga ggccgttcca tatttatcaa acgcatcttt
     3661 tgtcagactc tctgattttt tatagacatc cgtacgcaat tgcttaccca gtttctgtga
     3721 ttcacgagta gcaaaaaatg tatttgcgat agcagctaag atactgatca aagaaaagcc
     3781 gatcatcaca aaacctgtat gccaaatata atcgacatct ccttgcgcta cccctttatc
     3841 aataatattg gaggtaagtg tcggcaaata taaatctgcc agtacttgga tcaccatgaa
     3901 aactgcagct gcaattgctg aagtcaagga gatccgtttt acgattttta gcatctagtt
     3961 cccttctttc tatttatata ctccgttttt cagccaagaa agtttcaaat tgaattcact
     4021 gattgcttgc tctactgagt aatcttcaga tgttgaaaat aagcgatacg aatgcgcaat
     4081 tgttgtaaat acagtattca tcactaattg gatcagtaat tccaattcat gatcattttt
     4141 tactttcagc ttcgtccgat cgatcaattc aagcaactct tttgcatctt gtttgtgttc
     4201 tttatgtgga tcgtgaggcg ttttttggcg actcgtatga ggagcgactt ttcttgaggc
     4261 acggtaatcc atgttcataa acaagttacg ataaaaagaa gcattctcgc cttccaaaat
     4321 ctcataaatc attttagaaa aataggattc gaatcctttg aatagatcac catcggattc
     4381 tttcattagt tgtaacatat ctttagagct gtcacgtctc attgtctcaa agtaataaaa
     4441 ataaagatcc tctttatctt caaaatactg atagaaactg cctctaggga tatccgctaa
     4501 tttgacgata ttagcaatcg aagcttcttt taagggtact ctagaaaact cgattctcgc
     4561 agcctcgatt agtctttttt gtttttcttt aggcaaatgg aaaaatgtct gtgtcggcat
     4621 atggattctc ctttctttca agatgacaca ttgtcatatg acgctatgtc attataacac
     4681 ctcctacctg ttttgcaaga catttgcata gtttttttca ttatcatccg atacattaaa
     4741 aaagtgataa gaggaatcct tccatttgaa ttaatgagag ttgcccatta aaatagccac
     4801 atactaaaca aaggatgaga taaatggccc aatcaattag tgtccaagac ttcaataaat
     4861 tgcctctcga tgctgacagc atcgttttag atacaagaaa taccttagat tacacaaaaa
     4921 accacttaag ccactcgctt tctatccccg ctgctatctt gccaagacaa ttaaaaaatc
     4981 tagatccaaa gaaactttat tatattctaa gctatacagg caggcgatca gaagcattag
     5041 ccgatcaact tactgctaat ggttttcgtg ctgtttatat cattggtggg atggaagctc
     5101 ttcagtcgag tgttgcttga atattgcttg ttagattcaa aagaggctgt gacaaaaaat
     5161 cgtgtcacag cctcttttat accctgtctt tttttagtta tttattcttc aaccttatca
     5221 atatgacttg atagcctttt ctttttcgag taaactgatt ttgttagtca tagttataac
     5281 aatcatagca ataaaaggag aaggtaagca tatggcatct gttttagtag tcaaaggtca
     5341 tcccctaact gcagaagaat cgcgcacagt taaggcattg acgtcttttt taacaagtta
     5401 taaagaaaat catccagatg atgaagtgac tgttttggaa ctttatcgcg atgatattcc
     5461 ggaaatcgat gaagaattat tatcaggttg ggaagcatta cgtgctggtg cagaattcac
     5521 ttccttatca gaaagtcaac aacaaaaaat tgctcgtttc aatgaattga ctgatcagtt
     5581 ccttgctgct gataaaatcg ttatcgcaaa cgccttatgg aatctaaata tcccaacaaa
     5641 attaaaagct tggttcgata ccgtcaatgt agctggaaaa acgttccgct atacagaaaa
     5701 cgggccagaa ggattagtga caggtaaaaa agcactgcac atccaatcta atggcggtgt
     5761 atacaacgga caagactttg cctctcaata tgtaaaaggt attttaaact ttgtaggcat
     5821 cgatcaagta gatcagctat tcatcgaagg aatcgattat gaccctgacc gtgcagatga
     5881 gttgatgcaa aatgcattag ataaagctgc tgctttaggt aaatcatttt aaaaattgag
     5941 ataaatgcat acaaaagaga cagaggaaaa aatcttcaaa gcagcatttg tgccttggat
     6001 attttcccat ctgtctcttt ttgttattct ttctgctttt tcaacggatt ttcttcataa
     6061 agagaagaga ttgttgtgta ccattcaaaa acatgagtaa tcactacttt agctgctgca
     6121 taaacaggaa tccccaagat cacaccgatg attccgaaca atttcccaga agtcagcaga
     6181 actaataaaa tcgttactgg atggattgct aactgattcc ctagtactaa tggagagatc
     6241 aagcgtcctt ctattgtctg ctctactgca aaaacgatca gtactttgat gatcatgaat
     6301 ggacctgcta caatggctaa aaagatagcc ggaatcatcg ctaaaaatga tcctaagtaa
     6361 gggatcaggt ttaaaaaacc cgcaataatc cccaacgtga cagcatactc taaaccaatg
     6421 accgcaaaac caatcataaa catgattgcc acagcaaaag ccacagttag ttgtcctctt
     6481 atataagaag aaacttgact attcatttca ctcagcacat tcaacgtcgg tttttgcatg
     6541 cgtgtcggta aaaatttgac aaaataaggt gctaattgtt taccgtcttt caatagataa
     6601 aataagataa acggcatcgt cagcagagcc actaaaatag tcgctaccgc gccgacaaaa
     6661 cttccaagac tctgaactgt actcttagat atatcttgga tcatatcgcc tgccgaattc
     6721 atgaacttat cacctgctgt ttccaactgt tcgcgaaatt ggttgaacag cgggtcccgc
     6781 aaaatctcgg ttaatttatt atcaatcgta tctacatatt gagggaagtt actgatgaag
     6841 ctcactgttt gttcctgaat cttagggatg atcactacgc tcccccatgc gattagtgca
     6901 acaaccaaaa caaacagccc aatgatactg taaagccttg gtacttttcg cttttcaaag
     6961 taatcaacta ccggattcat caagtaatac aagattcctg ctaaaatgat aggcagacct
     7021 acgattgcta aaaattgcca gattggtgca aacaaatatg aaacttttgt aaataaaaag
     7081 acgatcagca atattaacaa tacaacaagt aaggctgtga ctacttgatt atttaaaaac
     7141 cagcgccaaa accacgatac cggtctgttc ttcttatttt ttccttccat aattcctcct
     7201 gttagttata aaaaatttgg cttcccaaac caatagcagg cagtctatgc ggatgaagat
     7261 agattccatt tgcaatttgt ccttcttctg gacaagcatc aaaaatcaat gatacgtgag
     7321 ctactgtctt taaattccca ttggccatcg gaccgacata atcgatcgta tatacctgat
     7381 catcaaaagc aattgttccg cctgctttca aagaaaaagg ttcgtctttt gatacttctt
     7441 gaatcaccga atattttcgt aaggcagctg ttgctgtttt atcgaacaaa atcagcatgg
     7501 gttcttcctt gtttattgct tctgtcccaa tttctgtaat ctttgcctga atcattgcac
     7561 tctctccttt aattgctttc aaaaacagta taacacattc tcattcttta gccgtgtgga
     7621 ataattatgt gatagaataa aaacgaaaac tatttcatta caattaggag gaatcagctc
     7681 atatgatcga aaagataatt ttaggaacct atacgcgtcg cgaaagtaaa ggaatctata
     7741 cgatcgacct tgatacagaa aaagaagaat tagccaacct ttcattagct gttgaagaaa
     7801 atagccctac ctatgtagca aaaagtaaag ctggcaatct ttacacagtt actagtgtgg
     7861 atggattagg tggtgccggt gcgtacgata aggattttca ttttttgaat gcggtaacag
     7921 agtctggcgc tcctttgtgt tatgtagcgg ttgatgaagc acgtcaatta ctatacgggg
     7981 cgaactatca taaaggtgaa gtaaatgtct accatatatt aggagatggc gaactgaaag
     8041 cagcagattc aatattccat caagaagcga caggtcctca taaaaaccaa gatcatgcgc
     8101 atgtccacta tacggatctg actcctgacc aacgattagt cgtatgtgat cttggtacag
     8161 atcgtgtgta tacttatgat gtgtccgaaa acggcaaatt gaatcttgta actaccttca
     8221 ctgcagagcc aggaacaggc cctcgccatc ttgtctttca tccaaatgga caattcgctt
     8281 atttatttgg tgaattagac agcacagtcc gcgtgctttc ttataataaa gaaacaggtg
     8341 cttttgaaga attacagaaa atatcgacct tgccagaaga attcgacggt gaaaatggag
     8401 gggcagctat ccgcatttca aatgatggca agttccttta tgcttctaac cgaggacata
     8461 actcgattgc tgtattcgca gtttttgaag atggttccca cattcaaaac aagcaaatca
     8521 tctcaacaga aggggatttt ccccgcgatt tcgcactaga tccaagcaat aattttgtcg
     8581 tgtgtgccaa tcagaacacc gataatctga cattatatcg ccgtcatcct gaaacaggat
     8641 tactggaatt actgaaaaaa gatgtctatg taccagaatg tgtctgtgtt tattttgatc
     8701 gttagtaaga aaaggagaat gaaatgaatc agcaagacgt attagacaga ctacgggaag
     8761 aactaaatat tcctttcttt gatgggaaaa tcgaagataa agagtatagc gaagaggaat
     8821 atcagaaact caaagccgat ttacagaact actttgaaca atatgtacag aatgtagaaa
     8881 actaatcagc tttccatact atctgaaaag aacatcagga caagactttt ccgtctatcc
     8941 tgatgttctt ttctacaaat aaaaaaccgc cgagatggtt agactcgacg gaaaaggagt
     9001 atttactcga ttttgagtaa gtacaaggtt gttgttggtt ataggtatgt cattatcata
     9061 aaggattttg atcactctat cataaaggta atattacatt tatattagat aactctagtt
     9121 tttattgtta tcaaagattt cttttatttt ttcaacaaca aattagtcat tttttggatg
     9181 gcagctgtca attggtctgt cgcttggtct tgttttgctt cagatgtatt gaatagatct
     9241 tcatctaccc ctgattcaag atcgatactt tggatcattt cttcacagcc tgctacatat
     9301 gaaagataag ctttttcaaa atttttatga atgcccattg cttttgctgg tggacgcaaa
     9361 gtactgactt tcataagcat tgcccgatat ttttttgttc cttcttcaaa taacaggatg
     9421 atctcttcaa gagtcgattt gtccagttca cttgtttttc cttcatcaat tgcgtttctg
     9481 atcacttcat atttcggatg cattccttct ccgattgctt ctgtttcctt gacgatttga
     9541 tcgatggact gcacataata gccaatattc ggtaacatgt tcgttttcct actttctcca
     9601 ttgttgttat tatttattct acttgttttt atcctcaaat gacaagtact tgaaaaaatt
     9661 taatggtttc tatacagctc gccattctta ttcgtgctca aagaaaccat ctgctaaact
     9721 atattgtttc cctgcgacaa tgtcatattg gatcaattga tattcgtcgt aaatctcttg
     9781 ttgcgagcga ttgaattgcg cttctttata ccactgtccg ttttgatagt acagttctcg
     9841 ttcaaacgcc ggaagttcct gttctaatga taaaagcagc tgataaaagc catttgttgt
     9901 ctgattcgtc tgttccatta gattcgctgc aaaataaaac ggactggtga ttgcatcgtg
     9961 agtcgtttgc ttttctaatt ctcctttact gtcgaacata agaaattgtg tttcatgcaa
    10021 tgtttgtttg tcattttttg cttggatcgt atctgaatag atccctggaa gatgatcccc
    10081 ccaaaaaaca actaaagtcc ttctagaaag attcttcaat tcttccgtga actgcttcag
    10141 tgccgtgctg ctataggaaa tatcttgcag ataattctct aagtcaagtg tcccacttcc
    10201 atctgaagtt tttgcaggat agtctagatt atcatacttt ccatcgtagg gcatatgtgt
    10261 ctgcatcgtg actaaatgaa tgaactgagg tgttttttct tccttgagaa gtgtcattac
    10321 ttctttgtaa gctgactcat cagaaatata tggattattc tctatcgtat ccgtataggt
    10381 catcgtacgc tcgctgatga actggtcaaa tcctaatgtc tgataaacat cctctctttt
    10441 atacatcgaa gtattgtatg gatggatcgc tgtcgtctgg tatgattgtg cgtttaacgc
    10501 tgaaacgata gaaggaagct gatccatttt tggtacaagc atcgtgtaag gggtggtgag
    10561 ttgcgcatta aataacgcca tagaaaaacc agtcaatgct tcaaattcga tatttgctgt
    10621 tccaccgcca tagttttgag aaagcatatt gccactatat gtttgatcag ctacttcata
    10681 ataatctgcc aatggctcac cagatacttc tacgccgttt aatcgactag gatctgagaa
    10741 actttcactc atcacgaaca cgatgttcgg cgactcttcc tctacagttt tatttttttc
    10801 atcggctagc ttctggtatt tttcagtgat ttctttgatc ttttctttcg aatatccttc
    10861 aggttcatcc attggttcta ccttcagatt gtacaaaaac ccaccgataa aaccagtgtt
    10921 atagtagttc attttctggc tgtaaggtat ccatagtgcc gttttattat atgcttttcg
    10981 caacaaatta tcgggattgt tgaaattact gatataccct agcagtccaa ttgccacaag
    11041 cagtgtcgtt aatcggatca attggaatcg ccttcctttt ttcaggcttc gaaacatgta
    11101 ccagcaaaac aatcctatca cggttcctgc ggcagctaag atcactgtaa acagcatagt
    11161 gccggtcata tctttcagca atccgatttc agtgatcatc ttcaaatcgt ctggataaat
    11221 cggttcttgc cgataactca ttttcatata atttgcaaaa cctaatacac cgatggctac
    11281 gacatacgta agggaaccta aagggacaga tcctgctagt gcaatcagaa aaataagaat
    11341 aatcagcaag acgagacagg ctaagaagaa tttttccgta tgccaagaaa aagcaaattt
    11401 caacgcaagg tctacggata gatcattttg acaccactgc aaaaataaat tactaaaaac
    11461 aacaagaaaa aagatactga tgattgctgc tgttcctttt aaaatctttt tctgtttggt
    11521 cgaaagtctg cttatatatc ttttcaaatg cttgctcctt catttctatc aataaaagaa
    11581 tgttgcttct cttattttag ttgtacgaaa ttcgaaagta aagaacggaa agaagaatta
    11641 atgaaatcca aatattctct ttctttatgg cctttctggt ttctttaata agcggacctt
    11701 tcgtactcca gaaatttttt ctagctgttg tttcacttga ttgagctgtt caaaatcagt
    11761 actgtctatg tcaatcaata tgtagacatg ctcattctta tggttacgcg atatatctgc
    11821 gatcgctagc tcgttctcat tcagcagaga aaatatctct gctaatatat tggttttctt
    11881 ttgataaaat atagtgaatc gaaacggcgc ttggaaaaac attcgtgcgg atggataatt
    11941 gaccgcttct ctaaccgtac cgaacaaaag ataatctttt aaggcttcaa ctgccaaacg
    12001 tccgctgtct gccaaagctt cctgagtcga tccccctaga tgaggcaaca taagtatacg
    12061 gtcattttct aagaactctt cttctggaaa atcagtcaag tatttagcca gatgttgttt
    12121 ttccaatgct tgcataacag catatttatc aacaatttca cttcttccta cattgataag
    12181 tacagcatct tttttcattt tttctatatt cgcctgatcg atcaatcctt ttgtatcttc
    12241 tgtcaacggc agcatgacta cgatgtaatc cgaggtagaa agaactgttt ctaaatctgc
    12301 ttgccgaaca taatccaact gggcatcgcg gatagaataa cccaagacat ccatccctaa
    12361 gctatagcaa cttagcgcta cttttgtgcc tatcgctcca agaccaagaa gaccgatggt
    12421 tttgccttgc aattctcgtc ccacataagc acttcttttg ttttctgcct gttctaaaat
    12481 attaggacct gttagtgttt gtaccatacg acttgcttcg attatcggac gggaagataa
    12541 gagcaaacag cacaaaacaa gctcttttac tgcattagca tttacacctg gtgtattcat
    12601 gacgatcgtc ccgttttcag aagctttttc aacgttgatc gtgttcaccc caacaccagc
    12661 tcttgaaatt gctagcaaat ctggtgtaag ccattcatct ttcactttcg tactacgaat
    12721 aactattcct tcggctggca aatcatctat tattttgaaa tattcccctt gtaacttttc
    12781 tatctcttgt tctggaatag catcaatcaa atgaacgtga tacataaaac cccctcctta
    12841 ctaacaagta tctcgaaatt tctgcaatta gacaactgtt taccatttga cttcctagaa
    12901 gcaaaaagag cagttagagc agagtaggct gagtgataaa aataaggcta tgacaagtta
    12961 ctgccataag ccttattttc gagagcaaaa tattgagggt gagatatctt gataaatggt
    13021 gttccgttag ctgacattga taataagact aaatttcaaa aaagttgaaa agcaaatttc
    13081 gtcaatctct tcttatttct taaaactaag tggctttttc acaacctctt tttatctaat
    13141 aaccactgtt tccaactcaa ctaattttgc ccacgtttca atttgatctg ttgttagatt
    13201 tagcgagaca acggtatgat gaccgccacc tgcctgaatc catgaatgga caccttcatg
    13261 gaaattcggt ttagttttcc ataacacacg tgcaacaggt aaattaggtg cttcagtcgt
    13321 tggttcaaag gcttctactt cattgatcag cagtttataa tgcgtaccga aatctgccat
    13381 cgatacgact accccttctc ctgctttccc atcaaataca agacgcgccg gatcttctct
    13441 atctcccatt gataaaggag aaacaacgat tcttggtttc gtcgcagcaa gcgtcggatc
    13501 tacttccatc atatgggatt ccaagattgc ttcttggcca ctagctagct cgtaagtata
    13561 atcttccatg aatcctgtat ctttccctcg agccatgatt ttcagcaacc gatcaatcgc
    13621 tgcagttttc cagtcgcctt ccccagcaaa accgtatcct tctgccatca aacgttgtac
    13681 agctagtcca ggtagttgtt tcattccata caggtcttca aaattagtcg taaaagcgtt
    13741 atagcctcct tcttctaaga aacggcgaat accgatttcc tgctgagctt gtacttttac
    13801 atgttcctcc catgtctttt cttcataatc accatagtcg aaggtataca attccttgta
    13861 ctcttcaaat aattgatcga cttcttcgtc tgatacgcga tcgattactt gtacaagatc
    13921 tccgatacca aaataatcaa cagtccaacc aaattgaatc tgtgcttcca ctttgtctcc
    13981 ttctgtgaca cccacgtttc tcatgttgtc accaaaacga gccactttta ttccaaaact
    14041 ttcattatag gctactgcaa catccatcca atcagcaatc tctttttgga tctcaggtcg
    14101 tttccagtag ccaacgacga ttttattttg tttatttaat cgagcattga tgaacccata
    14161 ttcgcggtca ccatgagcgg attgatttaa attcatgaaa tccatatcga tcgttttcca
    14221 aggaatgctt tcgttatatt gcgtagctaa atgaagcaat ggtttttgta gaagctttgt
    14281 cccgcggatc cacatttttg ctggtgagaa agtatgcatc caagtgatca cacctgctac
    14341 ttcttctcga taattgacct ctttcatgat ttttgtgatc gtatcagcag taactgctaa
    14401 ttcttgaagg cggattggat aaggcaattg gccattttca ttcagttcat cgaccatttc
    14461 ttgtgcatgt ttctttactt ctcgcagtgc ttcttcccca tataaatgct gtgatcctac
    14521 aacaaaccaa aattcttttt ctccaatatt caacataatt agccactcct tttatttgtt
    14581 ttgtccgtaa tacgcatttt ttccgtgttt tcgtaaataa tgtttatcta aaatccgttg
    14641 aggcagctct tctgaaaatg aattgagctg tctggtaaat aaattcatct tgcatacttc
    14701 atctagtacg actgcgttca ttaccgcact ttgcgcatct ttcccccaag taaatggacc
    14761 gtgtccgtgt aaaagtaccc ctggaattgc taagggatca attttgcgtt ctttgaatgt
    14821 ttctatgata acttttcctg tttcatattc gtacccgctg tcgatttcct gttgtgtcaa
    14881 aaatcgagca caaggtaccg atccataaaa agtatctgca tgcgtcgtcc ccatcgcagg
    14941 aacatccaat cctgcttgtg cccagatcgt cgcccatgtc gagtgagtat ggacgattcc
    15001 tccgatttga ggaaatgaac gatataaaac agcatgagta ggcgtatccg aagagggatt
    15061 gagtttacct tcaatgatat gattgtccaa gtccacaacg accatgtcgt ctgctgtcat
    15121 ctcttcataa ctgactccgc taggtttgat aacgaaatat cgtgtttcag gatcaaaagc
    15181 gctcacattt ccccatgtat atttgacgag tccgtgttta ggcaagtcaa gatttgcttg
    15241 gtatacttct tctttcagtt gttccagcat tttctatcac tcctttttct tgcaattgat
    15301 gaatcgctgt tttttcaatg tcgatcccct cacggtaacg tctcataaat atctgatagc
    15361 ctgctatttc ttctgctgaa ggtgtgatgg tgattccttg gtcttgtcca aacacgtctt
    15421 ctgccaagaa gttttctaaa gacttcccat gtcgatccat catataggct gctaataacg
    15481 cgattcccca cgctccacct tctccggctg tgtccatcac tgtaactggt gcctccatag
    15541 cagatgctag aatccgctgt ccaacttctg gtgttttaaa aatcccccca tgccccacta
    15601 aacgatcaat ttggacattc tcagatttca atatatccat tccgattctc attgcaccaa
    15661 aagcactggc taaatggatc cgcatgaaat tcgctaaatc aaaacggcta cctggtgtac
    15721 gtacaaataa tggccttcct tcatttactc ctgtaatgtt ttcaccggaa aaataaccat
    15781 aagaaagtaa tcccccgcaa tccgtatcgc cttctagcgc tttgttgaat aataccgtga
    15841 ataatttatc tctgcttatc tccataccca aagaattggt aaattcttca aaaatactga
    15901 cccaagcatt gatatccgaa gagcagttat tcgtatgaac catgcctact agttctcctg
    15961 aaggtgtagt gaccatgtcg atttccggat ggacattttc tagttctttt tctaatacga
    16021 tcatagcgaa ggcggaagta ccagccgaaa tgttccctgt ccctttggct acactgttcg
    16081 tagcgaccat accggttcct gcatcccctt ctggcggaca aaccgggatt cctggtctca
    16141 aatttcccgt agggtcgatc agataagccc ctgattcgct taacttgcca gcttcttctc
    16201 ctgctactag aactttaggc aaaatgtgat ccaagtcaat cgctacgtgc gctttttcga
    16261 ttaactgacc aaaaatattt ttcatttctt gatggtaggt tttcgttgaa ggatcgatag
    16321 gaaacatgcc tgatgcatct cctacgccta gtactttctc accagtaagc tgccaatgga
    16381 cataacctgc aagcgttgtc aaaaaattaa tttctttcac atgttcttct ttattcaaaa
    16441 tagattgata caaatgagcg atactccatc tctgcggaat attgtattga aatttttccg
    16501 ttaatattcg ttctgcttct tcagtgattg tatttcgcca agtacgaaat ggtactagta
    16561 actgttcctt gctatcaaat gctagataac cgtgcatcat tgcactgaac ccaatcgacc
    16621 cgattgtcgt aagattctcg ccatagattt cgaatacttc tgctgccatt tttcggtaac
    16681 tgacgcgcag tcctttccaa atatcttcta atgaataggt ccagatacca ttttctagtt
    16741 tgttttccca ctcataacta ccagcggcaa taggagtaaa agatgaatca atcaatacag
    16801 ctttgatcct agttgatcca aactcaatcc ctaaagatgt acgaccatct atgacatcta
    16861 acatactttg cgcatttact tccttcataa tgatttaact ccctccttct cgtcaaacct
    16921 gatcaaaccg tctttttagt agcgcttaca atgattattg tatacttttg tacgtacatg
    16981 tcaagcagtt atttacaaga atacaataaa aaacacttgt tttgtacgta cataagttat
    17041 aaaatgaaat tgcttacaaa atctgatcaa ccgccttctc gaaattctag aaggagagaa
    17101 tacttatgaa aaaagaaaaa aaagtttcct catcgtttat ttattttttt ggcgcatttg
    17161 gcggcattct atttggatat gatattggtg ttatgaccgg cgccttacct tttttacagc
    17221 atgattgggg tttggcaggt aaagcttctt tgataggatg gatcacgtct tcggtcatgt
    17281 taggtgcgat ccttggcggc tctttatctg gtcttctttc tgataagctt ggacgacgaa
    17341 aaatgattct tctttctgca cttattttta tggctggttc tgtcctttct gcatctgccc
    17401 ctcacaatgg ttcttatttt ctgattgcag caaggattct tctaggttta gctgttggcg
    17461 ctgcatctgc cctagttcct gcttatatgt ctgaaatggc tcctgctaga ctaaggggaa
    17521 gattgtcagg gatcaatcaa gtgatgattg cttctggcat gctcctttcc tatgttgcag
    17581 attatttgct aaaagggttg cctgaaacga tggcttggcg cgtgatgtta gggctcgctg
    17641 ctgtccccgc gctgatttta tttttcggtg ttttagctct accagagtca ccgcgttttc
    17701 ttatgcaatc tggccgcttg gaagaggcaa aaagagtatt gaactatatt cggacaccaa
    17761 aagaagctga acaggaattc gaacaaatcc agctgaatgt caaacaagaa aagacaaccg
    17821 gaacttcttg gcatactttg tttttagaaa aatatcgttc cttagtcatt gccggtattg
    17881 gtgtagcagt attccagcaa tttcaaggag ctaatgctat tttttactat attccgctga
    17941 tcgtagaaaa agcgactggg catgcagcga gcgatgcatt gatgtggccg atcatccaag
    18001 gaatcatttt agttgctggt tctttgctct ttttggttat tgcagacaag ttcaatcggc
    18061 gaactttatt gaaaataggc ggtagcgtga tgggactttc tttcatccta ccagctgttt
    18121 taggaactgt actggatgct catacaaatt cgttgttgat tctcttgttt ttatgtattt
    18181 atgtcgcatt ttattcttgc acatgggctc cactgacatg ggtaatcgta ggcgaaatat
    18241 tcccactagc tgtccgcggg cgagcatctg gcctagcctc ttcttttaat tggattggtt
    18301 ctttcctagt tggcttgctt tttcctgtga tgactgcttc tatgtctcaa gaaatcgttt
    18361 tcggcatttt tgggatcatc tgctttttag gggttttatt tattcaagag atcgtaccag
    18421 aaactcgtgg gaaatcttta gaggaaatcg aacaaagtgc atctaaaaaa acttatccta
    18481 aacgtatttc tatccatcat tcttaaagag gttgtgacag aagtggtcag cttcaagaaa
    18541 taagaaggaa ccgcttagtt tccagtcaca aggaacaatt tcaaaaaaca gcttctcatg
    18601 tttgatgaat attgtgactt gtgaccagtt ggtaggagaa caccgtttag tggagaggtg
    18661 gtgaaagaaa cgttcagcct tgagcgatga caaaagctct cacaaacatt gcgacgagta
    18721 accgtaggag caataagagg cggtaaaaac ccgtctctta tttttcgttt tctttcactg
    18781 tttcccgttc aactagtaca ggttcataga gaatatttgg ctgtgcctgt cctttttcaa
    18841 tcgttgtgac gatccaccgt gcggcatctt gtcccaactt ttcttttgga tgcgtcaatg
    18901 ttgtcaattt tttattccct aaatggctta aagaagaatc gtctgttcct acgatcgata
    18961 ggtcatctgg aatatgatag ccattttctg tcaacttatc tagcattaaa ctagctattt
    19021 gatcgttata gcaaatgatg ccatctaaag cttgttcttc tttcaaccgt tgtactgttt
    19081 tttcagcgat tgtttctcgt gtttccgtgg tgaaagtaat gatatcttcc gtcaaaaaac
    19141 gcacattata ctgttcacac gcgctgataa aacctttcat cctgtatttt ccttgaagat
    19201 cgtcgatttt tgtgatgaac aacagacgct gatgtccttt ttttatcaaa tattctgctg
    19261 ccataaaacc gactttcaca tcatctaaac aaatataaga ggcatttaac tcttcataag
    19321 cagcattgat catcaccatc ggaatgcctt gttctctcaa agcaacataa agtgctaagt
    19381 ttgggttata ctgattactt ttcgtaggct caacgatcaa accatctacc ccataagcaa
    19441 tcattttctt caaacactct ttctccaatt cgtgatcgtt attcgtactg gctaaaagca
    19501 atgaataccc agattctcgt aattcatttt cgattcctcg gataatcgac ggaaaaatat
    19561 aatcagagag atatgtggtg atgacaccga tcgtttttgt tctgttcaat ggtgtttttt
    19621 ctttcttata gctgtcatct acatatgtgc ccgacccttt ttctttacgc agatagccct
    19681 catttaccaa cacagcgatt gcttgccgaa tcgtatgtct gctgacgtca tatatttctt
    19741 gcagcttgaa ttctgaggga atggttgtat ttacttcgta ttcctcacga aggatctttt
    19801 gttttatctc ttctgcaata tcttgatatt ttgccatttg ccttgcccct ttttatttgt
    19861 acgtacatat atcataacga gtctgcaagg tttattcaag caaaaatcaa ggtttcttgt
    19921 gctcataagg catacggaac aggttcacca aattctggaa atccttgctc attccacgta
    19981 aagcattgcg cgttcgcgtg ccgattagga ttgtctaacg gatccccttt ttggttcttc
    20041 tctggtcgag catgatagat caaaatatca aagcacccgt cttctgaaac cgtaaacgag
    20101 ttgtgaccag gaccaaattg cttatttttt ttagaagaaa cgaatactgg ctccgataat
    20161 ttatgccatg attcttttaa taaaagattc ttgtgctcat cagcccacag caggcccatg
    20221 gcatagtttt catctgttgc actaccagaa tacgtaatga atattttccc attgcgagta
    20281 aggactgctg gaccttcatt tactgaaaaa ccccgttttt cccattcata ctcaggtata
    20341 gaaaggagga tctgcttccc ctttaacgtc caaggattat ccatttcgga tagataaaga
    20401 ttagaattcc cagtaatttc aggatcttgt tgggcccaca cataatatag ctttccttga
    20461 gaagagaaag ttgttccgtc taaactgaaa gtttcccttt tcgtttttat ttgtcccttt
    20521 tctatccaat ctgtattcat tggatctata tgctgatttt caagtacgaa catgcggtga
    20581 tgatgagatt catcacgtat agttttatga ttgcttgcag caaagtaaat ataccatttt
    20641 ccattgatat aatggacttc aggtgcccag atcaattcac tcatcggtcc ttcttcatga
    20701 gcctgccata caatcaggct tgcagcatgt tctaattcat tcagtgactt tgcccttctt
    20761 aattcaatca cttggtagcc tggaaccgat cctgtaaaat aataataacc atctgtatgt
    20821 ttatataccc aaggatctgc ccgttgccga acgataggat tattataaag tgtcttcatc
    20881 ataacaaatt tcctcacatt ttctttattc taattacatt ccaagagtat ttttctatcc
    20941 ctacatttaa ttcgtcatgt ttttctattg aaaaatagtt tatttcaggt tttatttttt
    21001 ctggttcttc acgtgtattt ttgtccaaca gatcgctact ggttaatgtg tagtgctctc
    21061 cttgatctag caaattgaaa tcatgttcaa tcgttatctt ttgttgttca tttgtgcgat
    21121 tgatcaagaa aatagtcagc tgattgccgt tatctacgat cacactgtcc acataaggaa
    21181 cattttttat cttttcacaa tcataaacag ggcaaatgat ttgttgtttc aatacttgac
    21241 ctttagcata tttcgataag tgttgcatca cataataagt tggattcctc catgcttgtc
    21301 ctttagcatc tgtcagtatt aaaggtattg tgtttactaa caatgattga caactaatct
    21361 ttacgcgatc tgcgtgtcgt aaaatagcca gccctaatga accaaataat aatgtatctt
    21421 ccatagtaaa gtaacaccaa tcgatgggac taccgacttc aaaatcccta tgtctttttt
    21481 ccggttgggc atgtacattc cattcatcaa acgctagata cattgttttc tcacttctca
    21541 aatatccttt tacataatca caagtagcaa cgatttcttc aatttgacga tcgaatcgat
    21601 aagcttttgc aaggtatgtc gctgtatcat cacgctcatt tttaggaggt ttagtcaagt
    21661 cttcttcatc atacttatca atatagttat gcaaagctaa ataatctaca ttctcataag
    21721 cttcttctaa tgcgatacga tcccattcag ggtacgatgg taaacgtggc gtagagcttc
    21781 caactaagat tgtttcaatt gaatcatcca ctaacttcat gacttttgag gcttcatttg
    21841 ctaacctacc atattcataa gcactcttag cccccacttg ccatggaccg tctagctcat
    21901 tccctaaaca ccataattta acgccaaaag gctttttttg cccatttttt attcttaaat
    21961 cactccagaa ggttcctttt ggaaaattac aatattctac taaatttttt gcttcatcaa
    22021 ttccttttgt tcctaaattc accgtgaata tcggtttcgc ttgtacatga ctgatccatt
    22081 taaaaaattc gtggagtcca aaagaatttg gttctatacc tcgccatgct aaatcaattt
    22141 tttttggtcg ttgttctttc ctacctacag tatccaccca atcataacca gatgtaaagt
    22201 ttcctccagg atagcgaatg acacctaatt ctaattcctt tacataagag aggacatctt
    22261 gtctaaaccc gtcaccatca gctgtttcat ggcccggttc ataaatccct gtatagacga
    22321 cacttcccat atgctcaaca aatgagccaa acagccgaga actgatctct cctaccgtaa
    22381 aatcctctct gatttttact ttcacttctt cgtccaacag ctttccctac ctttcatttc
    22441 ctatagtcaa gataatagat acaagtttcc atacgccacc agcaaagata aaaataaaag
    22501 gagggaaaag aaatatgagc agtacaaatc ctaataacaa tacgcaacaa agaatatttc
    22561 tcaaaaaatc aaaaaatact ttccatgcac tttgttcaaa aatttgtaaa aatgtgatat
    22621 ctttgttttc agaaatcaca tatatccagg ataaaagaaa catggaataa ataagtaaaa
    22681 ctacatatcc actaattgaa agtacgattg tctgttcttc taataattga ttaacaagaa
    22741 aataaataat gatcgttaac agactaagga acaagttctt accaaaatgt tgctttattc
    22801 cttgaaagaa aaaataaatg atatttcctg atccctcacc tttccattgc gccattacat
    22861 aaaaaacagc acatgtagca ggcacgatag taataacagg caaacacacg attacccaga
    22921 gaatacctaa taagatgtaa tcagaaacca caatcaactg tccataaagt ttagtctcaa
    22981 ctattttcat cttcctcact ctttcattcc agacatagct gttccttgaa caaaatattt
    23041 ctgtgcaaaa atataaacca ctagtaatgg cagcattgaa ataaccgttc cagccattac
    23101 aggtccataa aaagaaatgt tctgccctgt aaataaagct aaccctgctg gtaaagttct
    23161 catttttgaa ctacttgtca atatcaatgg ataaagcaag tcattccaac tattcatcag
    23221 cgtgaaaatc cctaatgata aaagagtagg tttgctcaat ggcaacatga ttttcaaaaa
    23281 aatattaaac tcacttaatc catcaatgcg tgctgcctct tccaaagaat ctggtaaagt
    23341 aataaaaaag gaacgcatca tgaagatacc aaatgcagtt gtcatacgag gaatgatcaa
    23401 acctgcataa gtatcaagca tgccaagcaa attgacttca ataaatagag gaatcatgaa
    23461 gacttggaat gggatcatca ttgtcagcaa aacgaaatag aacaaaactg attttccttt
    23521 gaaacgcata cgagcaaatg cataccccgc aagagaatca aagaatacag aagtgatcac
    23581 tgtccctcca gcaaaaatca ctgtattttt gatgtaatct aataaaggga tcgttgtcca
    23641 aaccttaata tagttatcta gggtatagct atctgcaaat attctaggcg gaaatgagat
    23701 aatgtctttt tcgtgtttaa aagaagaaat aatcagccac aaaaaaggaa acgcaataac
    23761 caacaatata actagcatac taatcgttcg aatgattttt ttaccactaa ctgcttttcg
    23821 actattcaca gtaactgtaa tcgagctttc tggtgccata caggtttccc tcctatttca
    23881 tttgtttttc tttccgtgac atgtacacat tagtggagat agctatagtt gcaacaatga
    23941 taaacaaaac cgaagacagt gctgatgcat agcccaattc ataaggtgct gtaaatcctc
    24001 gactgtaaat atattgaaca gctgtttctg tcttaaattg tgggccacca gcggttgcaa
    24061 catatacttg gtcaaacact tgcatcgaac caatcagatt cgttaaaata caaaaaccta
    24121 aggagggaac gatctgaggt agcgtaatgt taaaaaactg ctgtcgattt gttgcaccat
    24181 ccatttgagc agattcatac aaagagggag agattccctg aatagcagtc aagagaatcg
    24241 tcatagtaac accaaaattc ttccaaacag tcatgatggc taccgtcggc atagcaaggg
    24301 ttggatcgcg aaaaaattga gggatcggca agccgacttg tgaaaacaag taaggaatcg
    24361 cccctacatt cggatcaagc aacatagaaa aaataatcgc aattgacgtc aatgaacaaa
    24421 taactggaat gtaaaatgtc gaacgacaaa aacgattgaa tctggtattt ttagataatg
    24481 ctactgctgc taataaacca acgataattt gtgttggcgt ttcgagtacg gtgaaatata
    24541 aagaatgaat caacgaatta actgctcgtt catcactaaa tatacgtgtg aaattttcaa
    24601 aacttacaaa atttcttgat gtaaagaaaa tattcaaatc tgtaaaacta atagcaaatg
    24661 ttcctaaaag cggtatgaca gtaaataata ataaaactaa aaaagatgga gttaaaaaca
    24721 aatacgcagc acgatcagga cgattccaat atttggtgaa tccgttcata tagtccacct
    24781 catctttcat tttgataaaa tatacaatct tggtttaaac gaatctgaat aagatatccg
    24841 aacaatcgct gtgatttttg caataacacc tatctgcaaa tactaggtcg atcattcaga
    24901 tatctgtatc ttgccaacag tctatcctgt ataaagttct gtactatttt tgcagtaatt
    24961 gattgatggc atcatctgca tcgttcatga gtgtctcagg atcttctcct gctaataatt
    25021 tttcaatcag tggattgatg atatcgttat tgatcgcaga gatttgggta aaacctggta
    25081 aatatggctc tgcatagtcc atttgtttcg ataattcaga tacaatagga tcatttttta
    25141 cttcttcatc ttttgctact gaatccaagt aggcaggaaa accattttcg atactccatt
    25201 ttttaccaat ttcagttgta ttccaatatt tcaaaaactc atagacagct tcttttttat
    25261 tattatctgt acttgccgga atggcatatc ctacgccatc taaaatagct gtttttttgt
    25321 ctgaatcact cccttgtggt aaggtagtca cattataatc aatctcgttt gctttcaatc
    25381 cgttatttaa ccatggccca ttgatttcaa tggctagttg accagcattc attaaattgt
    25441 ccatttcggc gccagaaatc gattctggac cagtcttgtc tacatggatc attttttgga
    25501 tcgcttttaa cgttttaagg ttctcaggag acgcaaattc agacttagta taatcatcgt
    25561 tcactaattt accgccattt gctaacatcc agttatataa cggtgcagtt ccatccttat
    25621 tgaagacaaa acctgataca tttttactac tatcagttaa ttttggtgcc atttctgtca
    25681 attcttccca agtagtaggt ggttgttcag tatctaatcc agcttgttta aacaaatcat
    25741 tattccaata catatacatg ccttgtacct gcatggggac aaaatactgt ttaccttcta
    25801 cttcacctaa ttcaatagcc gtttctttaa aatcagattt atctactcca tcatattccc
    25861 agaaatcgtc taatggttgt accgctccgt tttctacata ctgtgcaaaa tctggataat
    25921 ttaatgcaat aaagtcaggt gcttttttag aagaaatcgc tgtaggaagt ttttcattca
    25981 aattggccca agtcatgata tccatttcaa ttttgatgtt tttatcattt gttttattaa
    26041 attcattaac gatttcttgc aatatttcac catcagatgc agtaaaccca ttccaaaaag
    26101 tcaaagtcac ttctttatca gaatcagatt ctgctgaact tccgccaccg caagctccta
    26161 aaatacctgt tgcagctacc aacaaagagc caattaacca ttttttacct ttcatttttt
    26221 gttcctcctt catctgataa acgacatctg accacattcc aagatagcgg tttcaataaa
    26281 caattgattt tcccatcttc agcttttaca tttgttgaat gacttggttt tacttgttca
    26341 cttttaggaa gattagttgc ttttgtatca aatccactca tttcagaaaa atcaatcact
    26401 ccctctaatg ataattcaga caagtgaaca gttagattca tttcttcttc tccacggtta
    26461 actgcaaata tgacaagttc tttgtcattt attacggcta tactttctaa atacggcact
    26521 tcttcaaaat cttccgtcgc atagtttgct gaacgaattt gtgggcttaa cacctctcct
    26581 tgtccataca cagcagcttg cataaatgga taatagatcg tctgtctcca agcttctccg
    26641 tttgtttctg tcctgattgg tgcaataaca tttaccaatt gagcaagaca agcaatcttt
    26701 actctatctg catgtttcaa taaagtgatt aatagacagc ctaatagtaa tgcatcttca
    26761 aaattatagt tatcttctag taaaggagga gcaatctgcc aaggcgctgc ttgctcatct
    26821 tgttccgctg aatgatacca aatattccat tcatcaaagg acaggttgat ctgcttctta
    26881 ctatgtttct tcgccttgac tgtatcgcac atcgctgtca cttcagaaat gaaccgatcc
    26941 atgtctaagg aacgtgcaag ataatgagaa agatcatttt tttggttgcc ataatattga
    27001 tgcagagaca aataatctat ttgatcgtac gcttgatcaa gaactgttaa ctcccaatca
    27061 ccaaatgtag gcatttgccg attcgaactt ccgcataaaa ccacctcaat tgaatcatct
    27121 acgagtttca tcgccttagc cgtttcctct gctaaccgtc catattcttc cgctgtttta
    27181 tgtccaatct gccaaggacc gtccatttca ttccctaaac accatgtctt aattccaaaa
    27241 gggctctctt gaccattcga acgccttaaa tcactccaat aagttccttt aggaaaatta
    27301 caatattcga ctaaattacg agctgcatct actccacgag tacccagatt gacagccata
    27361 ttgatttcgg cccccacatg ctgacaccag ttataaaatt catgaatccc aacttcattc
    27421 gtttcaattg tccgccaagc taaatccaac cttctaggtc tatttgctat gggcccaata
    27481 ctgtcttccc aacgaaatcc tgacacaaaa tttccaccag gatatctaat catgggaatg
    27541 ttcaattctt ttaccaagtg aatgacgtct tggcgaaatc cacttttatt agctgatgga
    27601 tgatctggct gataaatccc ttcatatact gctcgtccca tatgttcaat aaatgaactg
    27661 tacaaccgcg gatcaacttt gcctacacgc ttgacactac taatcaacat tgtggcttcc
    27721 ataactgacc tcctttaatt tatacatgaa taatacacct cgttgaaagc gttg
//
LOCUS       DADVXU010000029        27546 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.031, whole genome shotgun sequence.
ACCESSION   DADVXU010000029 DADVXU010000000
VERSION     DADVXU010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 27546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.031"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            30..677
                     /locus_tag="KB953_001157"
     CDS             30..677
                     /locus_tag="KB953_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBB6866145.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            761..1705
                     /locus_tag="KB953_001158"
     CDS             761..1705
                     /locus_tag="KB953_001158"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HBB6866146.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            1840..3291
                     /locus_tag="KB953_001159"
     CDS             1840..3291
                     /locus_tag="KB953_001159"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HBB6866147.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            3312..4148
                     /locus_tag="KB953_001160"
     CDS             3312..4148
                     /locus_tag="KB953_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBB6866148.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            4148..4807
                     /locus_tag="KB953_001161"
     CDS             4148..4807
                     /locus_tag="KB953_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBB6866149.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            4933..5622
                     /locus_tag="KB953_001162"
     CDS             4933..5622
                     /locus_tag="KB953_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866150.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            5628..6584
                     /locus_tag="KB953_001163"
     CDS             5628..6584
                     /locus_tag="KB953_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HBB6866151.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            6628..7140
                     /locus_tag="KB953_001164"
     CDS             6628..7140
                     /locus_tag="KB953_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB6866152.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(7396..7944)
                     /locus_tag="KB953_001165"
     CDS             complement(7396..7944)
                     /locus_tag="KB953_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBB6866153.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(8033..9214)
                     /locus_tag="KB953_001166"
     CDS             complement(8033..9214)
                     /locus_tag="KB953_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBB6866154.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(9450..10958)
                     /locus_tag="KB953_001167"
     CDS             complement(9450..10958)
                     /locus_tag="KB953_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HBB6866155.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            11117..11308
                     /locus_tag="KB953_001168"
     CDS             11117..11308
                     /locus_tag="KB953_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866156.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            11511..12428
                     /locus_tag="KB953_001169"
     CDS             11511..12428
                     /locus_tag="KB953_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HBB6866157.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            12421..13494
                     /locus_tag="KB953_001170"
     CDS             12421..13494
                     /locus_tag="KB953_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866158.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            13642..14160
                     /locus_tag="KB953_001171"
     CDS             13642..14160
                     /locus_tag="KB953_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866159.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            14141..14533
                     /locus_tag="KB953_001172"
     CDS             14141..14533
                     /locus_tag="KB953_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HBB6866160.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            14609..15544
                     /locus_tag="KB953_001173"
     CDS             14609..15544
                     /locus_tag="KB953_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HBB6866161.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            15677..16456
                     /locus_tag="KB953_001174"
     CDS             15677..16456
                     /locus_tag="KB953_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBB6866162.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            16449..16766
                     /locus_tag="KB953_001175"
     CDS             16449..16766
                     /locus_tag="KB953_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBB6866163.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            17010..18620
                     /locus_tag="KB953_001176"
     CDS             17010..18620
                     /locus_tag="KB953_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBB6866164.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            18777..19625
                     /locus_tag="KB953_001177"
     CDS             18777..19625
                     /locus_tag="KB953_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBB6866165.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            19856..20335
                     /locus_tag="KB953_001178"
     CDS             19856..20335
                     /locus_tag="KB953_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866166.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            20368..22596
                     /locus_tag="KB953_001179"
     CDS             20368..22596
                     /locus_tag="KB953_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBB6866167.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            22725..24368
                     /locus_tag="KB953_001180"
     CDS             22725..24368
                     /locus_tag="KB953_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBB6866168.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(24441..24986)
                     /locus_tag="KB953_001181"
     CDS             complement(24441..24986)
                     /locus_tag="KB953_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HBB6866169.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(25009..25494)
                     /locus_tag="KB953_001182"
     CDS             complement(25009..25494)
                     /locus_tag="KB953_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBB6866170.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(25484..26296)
                     /locus_tag="KB953_001183"
     CDS             complement(25484..26296)
                     /locus_tag="KB953_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HBB6866171.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(26435..>27546)
                     /locus_tag="KB953_001184"
     CDS             complement(26435..>27546)
                     /locus_tag="KB953_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HBB6866172.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT         9022 a         5020 c         5518 g         7986 t
ORIGIN      
        1 actattttat aacaaagggg atcgaatcaa tgaaaaaaac tgttatttat actagtttat
       61 ttgcagcttt gtttttagct gcttgtcaat caaataccaa tcaaggcaca tctgggaata
      121 cgtcttccag tagcgaacag cgctcttcac aggtatcgga ctcctcaaca aaagcaagca
      181 catcttcgtc tagccagaca aaaagttcta gtgaagcaag tactcagtca gatatcaaag
      241 tgtctgcagc tcgtgcgatc gaattgttcc aagataaata cccagatact gcaattacta
      301 gtttggaatt agatagtgat tggggatctt acttctacaa gatagaaggt gtcgatgatc
      361 aaaaagaata cgaagcaaga attgatgctc aaaatgaaaa aatggagaca gagaatccag
      421 aaactctaga tcgagatgaa caaaatggtc aaaagaaaaa agaagatggt ctggatgttg
      481 ataaattgat cacgattgag gaagcagcaa aaatcgccgt cgacaaagta ggaagcggta
      541 cggcaacaga ttgggatttg gataaagatt taggtactac ttattgggaa gtcaaagtaa
      601 aagacggcaa tcaaagtaca tcagtcaaga tcaatagcca aacaggcgaa gtactgtcaa
      661 ctgaacaaga tgattaagaa tttcttcgga aaacaagagt tgatttttct tttagtcatg
      721 atacactaga caaaataact tgtttgaagg agcgatttac ttgagtttag aaacgacctt
      781 tgccaatcat atctttgcca atcctttgat gaatgcatct ggtgtccact gcatgactac
      841 ccaggaatta gatgaactgg ctcattcaga agcaggagca tttatcacta agagctgtac
      901 gatcaatgaa cgaaaaggaa atcccgaacc acgctatttt gatgttcctt tagggagtat
      961 caattctatg ggacttccaa acttaggttt ttcttattat ctagagtatg ctcttgcgta
     1021 tgaaaaagtg caggaaaacc aaaatcaacc gttatttttt tccattgccg ggatgagtgt
     1081 ccaggaaaac ttggaaatgc ttgagaaaat agaaaaaagt gggtttaatg ggatcactga
     1141 attaaattta tcttgtccca atgtgcctgg aaaaccacaa ctagcttacg attttgaggc
     1201 cacttatgaa acactgaaag aagtattcag catcttttct aagccgctag gaatcaaatt
     1261 gcctccttat tttgattttg ctcattttga tcagatggct gatatcttga atcagtttcc
     1321 attgacctat gtcaatgcta tcaacagtgt gggcaatggt ttgtatatcg atacagaaca
     1381 agaagcagta gtcatcaaac caaaagaagg gttcggcggc attggcggag agtatatcaa
     1441 gccgactgct ttggcgaatg ttcgcgcttt ttatacacgt ttgaaaccag aaatccaaat
     1501 cattggtaca ggtgggattc ggacaggaca agatgcattt gaacatctgc tatgtggcgc
     1561 aagcatgtta cagattggta ccgagcttca taaagaaggg ccggaaattt tttctagaat
     1621 cataaaagaa ttaacgcaga ttatgtcaga aaaaggatat acgtcaattg acgaatttaa
     1681 aggcaaatta cgcaccattt catagcaaag agacgaaggt catgattgat cttcgtcttt
     1741 ttttataaaa aacactcgtc tgttcattcc tgtgcaagcg tactcattgt atgttagacg
     1801 aacttgtggg aaaataacag aggcaaaaag gaggataata tgaatgatgc aacagaatta
     1861 gcaaaagagc ttaaagaaaa agaatttagt tttcaagaac tagttacgga tatacagaaa
     1921 aaaataaaga agcaaaatca tgaattgaat gcgtttgtcg cgcttgaacc attcgagagt
     1981 aattcccacg agctattgat tccccagtca gaaaaagggc cattggctgg tatcccattt
     2041 cctttaaaaa tgctcggtca agaaaaaaag ggatggctag ctacttcagg atctcgttta
     2101 tttgaaacac accgagcttc tcatacatct aattatgtgc ggcaagcaga agcgataggt
     2161 ctagttcctt ttggacaaac caatgcgcca gagtttggtt tcaaaaatat tacagaccca
     2221 gtcatctatg gcccagcaag aaacccttgg aaccttgaat gtacaccggg cggatcaagt
     2281 ggaggagctg ctgctgcagt ttcatcagga atcgttcctt tagctggtgc tagtgatggc
     2341 ggtggttcta ttagaatacc cgcttcgttt tgcggattga ttgggctgaa accttcgcga
     2401 gggacgatgc cagttggtcc atatgcttgg agaggatggc aaggagctgc tattgatttc
     2461 ggcttgacag tttctatgag agacacggag gctttatttt atgggatgag aagtatccat
     2521 tccggtgcgc catatcaagt atcgccagtt gagtggcaaa cacatccaag aaaaaaacgg
     2581 ttgaagatag ctttttgtgt tgattcaccg atccactcac agattagtga agaggcagta
     2641 cttgctgtaa aaaaagctgt agatttcctt ggaaaagaag ggcatgattt gacagagatt
     2701 ccttacccac tagatggtcg taaactgatc acgtcttatt atcagatgaa tggcgcagaa
     2761 acagcagcaa tgatccgctc gattgaaaca ggcatccagc gagctgtcag aaaagaagaa
     2821 cttgaaccca tttcttggac tttgttccaa tatggtcaga agctctctgc cgctacttac
     2881 atcgattctc tgcatgtatg ggatgaagca gcaattgtca tggaaaacct acatcaagaa
     2941 tacgatctat tcctttcacc aacgactgca tttaccgccc ctaaaatcag cgaagatttg
     3001 caaagtgatg cgataagagc acagatggaa cacatcagtg attgttctga agtagaaggt
     3061 ttagacgttg tttcttccat gtttgaggag agcttgaaga tcacacctta tacacagttg
     3121 gctaatctga ctggacaacc agcaatcagc ttgcctactc atttgacaaa agaaaacatg
     3181 cctctaggaa tccaatttat ggctgccaga ggaagagaag atttgctatt tcaaatcggc
     3241 tatctatttg aagcaaatca tcagttccat ttaccaaatc catggaaata gcagaaacaa
     3301 ggagaagaag aatgaagaaa aaaagattgt ttgctctaat aactagtgcc ttttttattt
     3361 taagcgcttg cggtacagct gccgctaatc aggaagataa cagctggagt aatgtagaaa
     3421 aaaagaaaga attagtagta gcgacttctg gcacactgtt tcccgcatct tattataatg
     3481 atcaaaatga attagttggt tatgatgtag atttggcaaa agaggtggct aaacggttaa
     3541 atgtcaaaat ctcctttaaa gaatacaacg tggatggaca aatcacttct gtttcaaaaa
     3601 gagaatcaga ttttgctgcg aacgattttg gtatcacaaa agaaagagaa aaaaaattct
     3661 ccctttcaga gactttcaaa tattcatttg acagtatgat cgtacgaaaa aaagatgatt
     3721 caggaatcca ctcacttgaa gatcttaaag ggaaaaaagc agctggcgaa cccaatacta
     3781 gctatatgcg tttagcggaa aaacacggag ctaaattagt cacatatgac aatgccacga
     3841 atgatcagta tttaagcgat gtagcaaatg gtcgaacaga tgttattcta aatgattatt
     3901 atctgcagaa aatggccgta tctgctttac cagatatccc tgtaaaaata ctggagaacg
     3961 tttatttcaa tgaaaacgaa agtggttttt tattgaaaga aggaaatgat gctctgaaaa
     4021 agaaaatcga tggcgtatta aaagagatga aagaagatgg gacgacaaag aaaatatctg
     4081 aaaaatattt tcaaactgat gtttcagtca aaccgaaaga aacgatggct gagtcggtga
     4141 gtgagtgatg tactttcctc taattgattt ttccctcatg tggacttcac ttcctttcgt
     4201 actgcaaggc ttggtataca cattagggat tggcttcgtg tcgtttcttt taggaaatct
     4261 ggtagggttg cttttgacag ttttaggtct tttggattgg ctgcctctga atgtatttat
     4321 tcgtttttat ttatcgttct ttagaggcat tcccgcacta gtcctgctgt ttctgttata
     4381 ttttgggtta ccttatcaat taagtgcctt aacggcttca gtcatctgct ttacgattac
     4441 cagcagtgca tttattggtg aaatctatcg aggatcacta gcaggcgttt cttcaggtca
     4501 gtgggacgct ggtttagctt ctggtttttc tttctttggg gtattgaaat atatcgtttt
     4561 gccgcaagct tttcgaatct cagtaccggc actcagtaac gtagctatgg atctagtgaa
     4621 aggtacttcg ttagcagcaa caatcacagt gccagaaata tttcaaaaag caaagatcat
     4681 aggcggtcgg acattcgatt atttttctct ctatatttta gttgcactga tttattgggt
     4741 catttgtatc gggatcgaaa aagtgcaaca gtatttagaa aaacgttcta tgcatcagtc
     4801 aatctgaaaa atagaaatgg tgctagcgtg gctggcacca tttctatttt ttgtttcttg
     4861 tatattctgc ctagtatttt aaaaccattt catgtaaaat gtaaatcgaa aatatgttcg
     4921 gaggtagaaa acatgaagac cccgctaacc atcgaaatag aatcctcgtt ataccaatat
     4981 tgcatcgagc aaggcggctt ggttgtagaa gaagtcacga tgcctgatga ccaaggaatt
     5041 gtagatacgc ttagctgctt gacaaaattt gatggaagcc gagaatggcg atgctatgag
     5101 ctgaaagtga caaaagcaga ttttcgctct acagccaaac tgtcatttgt tggacattat
     5161 aattattttg ttttaccaag taaactatat gaagaagtaa aaactgaaat ccctacagga
     5221 ataggtgttt tagtgtatcg tccgtacacg gttgaagagg agatgcctgc ctctggtaca
     5281 tttctgattg cgaaaaaagc agcaaagaaa gagcttgctg taccggaaga agctttgaca
     5341 aaccgcttca tggcttcttt attcagggaa gtaagaaaag caaagcaaat ggagcgaggc
     5401 gtaaaatatt ttccaacaga tcaattgtac aaagaattga aaagacgttc agaaaatcaa
     5461 gatccatttt ctaacgaacg atattatgaa cggtttttag aggaaatagc tagtcaggca
     5521 atcaatgagt tgcaagaaga agttatcgcg cttcagcatg attatgaata tttgaggcaa
     5581 caacagcaga tccgtcggtt gcctacagag ccattggagt gaaaaatatg tactatcctt
     5641 atttaagagg aaaacaattt gacctattag cattaaaaga agcactgtcg cgaggattgc
     5701 tctccaataa aatccaacca gtgatcgaac cagtacgtga ttccgctacg cttaaaaatg
     5761 tgatcgaatt atttcaaaaa aagcaccatc gaatcgctgt gatccaaaat ccccaagtag
     5821 gacaattcaa attattcgat cagcatgtgc acagttggac agtcaaagaa aatagctctg
     5881 tcgtccctgc acagatctta acgccagaaa atctcagtga agtactggat tcacctccag
     5941 cgtttcttgt tttcgatgga cagcactacc caaaagatac agaggtgtgg aaacagttgg
     6001 caggagttga ttcgaaattt ttgataccag atactagtcg ctttcgcatc tggctgcccg
     6061 aaaacaaaat tgtgatccgt gactcttttc aaacgagaaa gcatgttgaa agttatgcag
     6121 ataaaacaga tgactttttt agtgatgact atcttttttt ccacgcagac ggatatattg
     6181 gattttctga tttcacgatc gaaggtagcc gttatttcga taaagggttc cctagccgag
     6241 cgcttgctat ccatctaacg tatattgatg cttacggcaa tatacgcgtg aaacattttg
     6301 tctctgacag taacgactct gctaaagacc aagcattgaa gtttcttgaa gcaggagata
     6361 aaatgaaaaa atggatcatg cgtcaccatc atcaactctt gattacttca ggaatgattg
     6421 aattattggc tctttaccag caacaaaaat tccctggact gggagtgttg aaaaagtggt
     6481 cacttatgca tcatttggaa cttattagtc acctgctaga tcatccaaaa gattggctta
     6541 aatattattc aaaagttcag gaactatatg aagtgatcca atgattagat aatgtaaatg
     6601 aaaaaggaat agaaaagagg aaactggatg tatattcggc aaacaaggaa agaagaagta
     6661 gaagaagtgg tagaaatcat taatgccgca gcggatttca ttgcttcgca aggctctcct
     6721 caatggcaaa acgggcagaa accaacaaaa gaaacgattt tgaacgatat tgaaagaaaa
     6781 gaaagctatg tattaatagt ggaagatcaa attgctggaa cagctgcatt agtctcaagc
     6841 gtggatcccg tttacacaaa aatagatggt agttggaaag aatcaactgg tccttattta
     6901 tcgattcatc gtgtcgcttt agcagaaagt actcgaggaa aaaaactagc aaaaccgttc
     6961 ttagaacatt tgttgacgat tgcagccagc aaaaatatcc aagatatccg tatcgatact
     7021 tatccagcta atgaaccgat ggaaaagacc atctattctg ctggattcac ttatcaaggg
     7081 atgattgaat ttccttttga acacggcgaa agaaaagcat atcaaataat catcaattga
     7141 ctataaaatc atctgagcaa agataaagac attaacacta ctgaaaaaaa caatcatatg
     7201 atagccaaga ggttgtaaca gaagtggcca gcttcaaaaa acaagaaaga atttccgaaa
     7261 atgcttctca aatttttgtg gaattttagc gtattttacg aaatagctgc tttctgaaac
     7321 tcccttcatc gagaggtcgt gacatagctt ttgtcacgac ctctcggctt atcctacgag
     7381 tattattcga tacatttata tcaataatcc tttttttatt ttatctgcag cttcttggaa
     7441 cgctccatcg tttgacacga ctaaaagtga ggggatagct ttcaaacgtt tcaactgtgc
     7501 taaatcttct acataaaccg ccagtctggc tgaaatctgt tcagatgaat ctccacgttt
     7561 tttcaggcgc gattgcaatg tttctttaga gatcgtaata aagataggac ggactaccgt
     7621 cccgaaatac tcatataaat gccaaaaacc atttgctgtc aaaacggcgt aacaatttcc
     7681 tactgtcagt ttctttttaa tttctgcttt actggaacca tatttatgtt gagcatattg
     7741 gtcccactct gcaaactcat ttcccttgat catctgattg aatgtattgt cagaaacaaa
     7801 ataataatcc tcttgatctt tttccccagg tcttggtgat cgattcgtgt aagtaatgat
     7861 cttttgttcc ggctgaaaaa tagcttctgc gagtgaagtt tttccagaac cagaaggacc
     7921 gataaataca tagcaataat tcattgtcat ttctcctttg atgattgaac aagtgaaaaa
     7981 gctttggaaa atatttccaa agcttttaaa agtaactttg cttttttgta tattatacca
     8041 gcaagccagt cacaatagca cttaaaaagc tgactaaagt cgctgttagc aaaattttca
     8101 atccgtgttt tgcgacaaga tttccttttt cttcatttaa tcccttcatc gcaccagaaa
     8161 tgatcccgat agaagagaag ttggcaaatg aaacgaggaa gacagatata attgctgttg
     8221 tacgctcagt aaaatggaaa ttaccatttg ctaattctgt catagcaaca aattcatttg
     8281 taaccagttt agtcgccatg atactaccgg catccactgc ttctttccat gggattccgc
     8341 tgatgaaggc aagaggggca aagatgtaac ctaggatctc ttggaaagtg atgccgaata
     8401 tcccgtggaa gatagcattg atcatggcaa tcaaagcaac aaaaccgata agcatagcag
     8461 caaccgtgat cgccacatgg aaaccatcaa gaatgtattc tcccaacatt tggaaaaatg
     8521 tctgttcttt gttttcttca atgatcagct catcttcttt ttcttctaat acatagggat
     8581 ttacaattga agccagaata taaccgccaa ataagttcaa aacaagggca gtgatcacat
     8641 atttagaatc gatcagtgcc atataagatc cgacgatcga catagaaact gtagacatag
     8701 ccgatacact catggtgaac aaacgtttct cagataggaa gggtaattcc ttttttatgg
     8761 aaataaaaac ttccgattgt cctaaaatag cagaagctac tccattataa gattccaatt
     8821 tgcccatccc gttgatttta cttaatccta gaccaacagc tttcataaat aatggaagaa
     8881 tcttgatata gcgtagaata ccgataatag cagaaataaa tacaattggc atcaatacgg
     8941 aaagaaagaa aggtgcactt cctttatttg ctacaccggc aaaaacaaag tcaacacctt
     9001 gcccagcaaa agcaagaaga tgatcaaaca tttttgccaa tacagaaaca accgctgtac
     9061 caaaagttgt acgtaataat acgaaaccaa gaacaagctg taaaacgaac atcacaatta
     9121 gtggtttcaa tttgattgct tttttgttac tgctgatcaa aaaagcaagc cctaatgtga
     9181 caaataaacc aataatacct aataaataac tcaaataatc actcctattc caaattccgt
     9241 tttattttac ctgttttgca aataaaatac taatctttag tgaaattttc attacttttt
     9301 tagagtgaaa cgcattcatt ttccattaga aaataaagcg aacaaaataa aaggtttaaa
     9361 aagtgtaacg tcttagtaaa gtctataaaa aaacgaaact atcgattaag ctaatagttt
     9421 cgttttcaac atttataagt aaactttttc tattggtaat atagattttc ggaagggtag
     9481 acaggtgcgc aggtaatatc gattcctagt tttttcaatg tttgttcatc atttttattg
     9541 accatcacag tagagtgggc ttgtgctccc tcaagttcag ctagtttatc ataagcaact
     9601 tgagcgattg gattcgttac tgcactgatt gataaagcga tcaagacttc attgatgctt
     9661 agtgcatcca aagaggaatg aagtgtcttt gatttcaatc cttgtattgt ttcaagaact
     9721 aatggtgaca ataaaggaat gtcatctgcg atatgggcta aatgttttac tgcatttaag
     9781 ataactgccg ctgacgcatc catcaatgaa cttgtacgcc cggtaatgat gacttcatct
     9841 ggtaattcga ttgcaatcac agcagaagga tcttttgtag gactttgcgc ttgaatctta
     9901 tctgcatagt catgggcagg gactaccacg tttcggtctt caggacgtaa accgacttct
     9961 tccataatca gtttgattcg attaactgct tcttcgtcga tcaatccttt tttgaagtcg
    10021 cattctgttt ggaaataacg acggatgatt tcttgtttag aggcttcttg gacaacggta
    10081 tcatccacga tcccaaatcc tacacgattc acacccatgt cagtaggaga ttgataaaca
    10141 gattctttac cagtaatttt ttcaataata cgtttgatta caggaaatgt ctcaacatcc
    10201 cgattatagt tcacagctac tttattgtag gcatcaaagt ggaaggaatc gatcatattg
    10261 acatctttta gatcgactgt ggctgcttca taagcaatgt tcagcggatg tttcaacgga
    10321 acattccata ctgggaaagt ttcgaacttg gagtaaccag ctgcatgtcc tttttggctt
    10381 tcatgataca gttggttcaa ggaagtagcg agctttccac ttcctgggcc aggagctgtc
    10441 actacaacga ttggtttagt tgtttcaata tactcatttt ttccaaaacc atcttctgag
    10501 acgatttttt ctacgttgac tggatagtct tcgatttctg agtgtttata gaccttgatg
    10561 tttcgtcttt ctagtttatt gataaagact ttcgtggcag gctgtccact atagcgagtg
    10621 ataacgacac tgttgattaa taagccataa ccacgtaatt catcgattaa ccgcaagata
    10681 tccatatcat aagtaatccc ataatcacca cggatcttat tacgttcaat atctccagca
    10741 tagacgcaaa tcagtatttc agcttgatct ttcaattttt gcagcaattt gattttcgca
    10801 tcttcttcaa atcctggaag tacacgtttc gcatgcttgt cgtcaactag cttcccacca
    10861 aattctaaat ataatttgtc atagtggttc actcgttcca aaatatacgc tgactgctct
    10921 tcaatatatt tttctgaatc aaaaccaatt tttttcacta ggttccctcc ttaagtacta
    10981 aagcattttt tcattttttt gaggattcgt caactggaag agcgaaattc ctcaaaaaaa
    11041 caaagaaaat ggtttaaacc aacttgtcta gatagttatt cattggtatg atagtttttg
    11101 ataaggaagg tgctacatga aaaaaagaca gaaaaagaaa aacgcatata aacactatat
    11161 ccgttcgatt tttaccggtt atgaaagaat gcttgaagat cccgaattgg aacaattgac
    11221 gtttacttat ctaaacgaag aaacgcagct aactcgtgat gagcatcagc gcattcattt
    11281 cacaacaaga gatctgccta gtaaataaaa aataagataa aggtcatcgg attcgattct
    11341 gtcctatcag atggaaagac tagttgatcg aaaaaaaggc tgtaccaaat tttggtgcag
    11401 cctgttttat ggagcattgc ccgctctttt ttataacaat tttttacaga aaatggtact
    11461 attgaagtat taataagtga aaaacagatg agtaagagga ggcaagtagt atgaaagagc
    11521 cagatacaaa tgcaaattat gagccagacg aactagagga gacgaatgcg gatcaggata
    11581 caggacaaga atcgtcttct ttttacgata tcaaagaaaa agcaagcatg aatctttccc
    11641 aattattaga agacttacag gcttttgaaa aagctgtccg cgaagaagat atccctgaga
    11701 tctaccgttt atacaatggc cgattgaatg aagaattaaa aagaagctca aatgaaaatc
    11761 acgagatcga tcaattgctg atgcgtaaga tccatgatcg ttttttacag gaatttcctt
    11821 ttatggaaca agtagaaaac atctcgccaa cgatgagcta ttacaaaatc ggcacttatt
    11881 accatgatcg gcccacggtc ggtattgatg caagtctgcc agaaattttt gttttgccta
    11941 agatcgatga agagtgggag aaatattccc aaccggagtc aacggactat gacaaaaaaa
    12001 tcaatgagct agacgcaaag gtgatcactg ctcagacaga aattgaacgt ttagatgaac
    12061 aaataaaaga aatcaatcga caaatgactg atttgaacga caataaagga ttcttgaatc
    12121 gtaaaaaaat cgaggaagaa attcaagaat tagagaagaa gaagcaagta ctatctaatg
    12181 aaaaattagg ctggcttcct tatattgaga cgcctgaaac aatccagcag caaaaagaag
    12241 cattgaagca agaagcaaga gcagaccagc ttcgtgcagc catcgtagaa aaagaacaaa
    12301 ggcaaatcag acgctatttc ggtagcaaag agggattcgg tcaagcaatc catgaattct
    12361 tgatgaatta tcttggcaat gagaatccta ataatcaatc taatgaagga ggttccgagt
    12421 atgaatgaac acgatgaagc agaatggaca aaaaatgaag aacaaacaga agatcatgaa
    12481 gaaacgaaca cttcattgga agaacaaaca agtaatcctc tggatgacac agaagaaatc
    12541 cctatgctag aggaaacaaa aatacacgaa aaagaaaaag taactccgac gaatacgaat
    12601 actgcagaaa acacccatgc ttacgaaata actgaaaatc ccgggtattt agaaggaaaa
    12661 gagcaagatc atcaagatat cgtcagtttg attgaaacag cggaagcaga attagcggat
    12721 ttacgtatga gaaaagattt gatcgaatca gattttccac aattattaga atcatatgat
    12781 cgttttatat tgaatgaaga accaatctcc acgatcggaa agacaagcct gatcgaatac
    12841 ttcacgtatg aattactgaa accgttgaat gatattggtc tggaacggtc aaattcagct
    12901 tatgacacat ggaaaacaag acatttttcg atcaattata cgttgaatgg acaaaacgaa
    12961 ttagtctttt cctttgtatt accgaccatc gatcaacgat accaagtgga atcgatgagt
    13021 ctcctaaacg tatcaccaga gacaatggaa atcaatgtcc aagatgaccg ggtgctttct
    13081 ttgatccgac actggtcagt ggatcgagta ttctctgcag ggcagatcac gatattcaat
    13141 cacaaattga atcaattatt agcccatgca agaacacttg gttttaccgt taatcagaca
    13201 ttactagaca atacgaagcc acttcgtttg aatcttcaga gcgaatttga attgacgaat
    13261 gaagtattgg atgatatttt tatcgtggca atgaaaaatc catcctacga tatggaaaag
    13321 atcaacgaaa ctacttatca agtacgatta gataaaggac agtctttaac gataacaaca
    13381 aacgaaaaag atcaaacagt cttgtcgatc tcatctggag actacccacg atctgtcatt
    13441 gatttcttta tcaattacga attcttggtc ccattaatgg tgagaaagtc ctgattggat
    13501 aagttggatt accagatgtc attcagataa ttctgttcca aaggacttgc ggcagttatc
    13561 cagaataatt gtcacaagtc ttttctgtat gtactaaatg gacgtgaata acttgctgat
    13621 gaaggaggga tttcattagg aatgtccaca aataaaatag cacagcttag cttattgtct
    13681 gctgcttgtg tagcaggtag gatcgctttt caatttattc cgaatttcca gccaatgact
    13741 gctatttttt tatttatagt actttactta agcttaaaag atgcgttaat tgtcatgagt
    13801 ctttctattg cgatcagcag tttttatttt ggtgttggac catgggtcat cggccaatgg
    13861 atcagctata ctgttgttct gagtttgttt tcaatcgtct gcttgagaaa aacagtgcaa
    13921 aggaatctat ggttaaaggg agtctgtttt tttctggcgg gcatggttta cggaataggt
    13981 atgaccgttt ttgatacgct gttatatcgt ttgcctcaac cttggatcta ttaccttcaa
    14041 ggggtgtcgt ttgatttgat gcacagtata ggcaatgttg tgttctttct tgtttttctg
    14101 ccagtcgtca aacgttttta caatcattca ggaggaaaaa atgaaaaaaa taatttatag
    14161 tttgcttgca atgagtgtca gtatactcat tttgacaagt tgcagtacaa cctatacaca
    14221 atcggcttcc aaccaaaagg aggaagtaaa aacatcgcaa aaagcaacaa tcacgttaca
    14281 agaaaatggt caaaactttt cggaaaaaga agtgttcttc aaaaaaggcg atgatttact
    14341 gagtattttg aaacgaaatt ttgaagtcaa agaagataac ggcttcatca cttcgcttga
    14401 agggcactca caggatgaga aaaatcaact ctattggatg tttactgtgg acgggaaaag
    14461 tgctactact ggtgccaaag agatcaaatt gaatgatgga caatcggtca ttttcaattt
    14521 gtctaaacta taagtttcat aaaaaactgt taaagggcga atattgccat aattcagtga
    14581 gttcagttga tgaaagaagg aaaataacat ggaaaatttt aaaaaaagcg gaatgttttc
    14641 cgtaattgct gctctagggg ctaatattct agttgctatc agcaaatttg tcggctatgc
    14701 aatcagtggc agtgccgcca tgctaaacga aagcatccac agtatcgttg attgcagcaa
    14761 tcagattttg ctgttattcg gtgataaaca agccaataaa ggacagagcg agttgcatca
    14821 atttggtgaa ggacgagcaa aatacttttt cagtacaatc gtcgcaatga tgcttttctt
    14881 cggcggtggg gcacttgggg tgatggaagc ttttgaaaag ttgacgcatc catcccatga
    14941 agtgggtaat ccgatcattg ttatagcaat actgttattt ggtttggtaa tcgaaggaag
    15001 ttcgttacgc gtggcaatga aagaaatcaa agaattgaat actgaaaaac tttccacgat
    15061 gcgctttttg cgcgaaagtc gacacagtga aatactgatt atttttacgg aagacttgtg
    15121 tgctgtaatc ggtttggtct tggctttagc aggaacaata ttgacttatg caaccgggat
    15181 tgctgcattt gatgcgatca gcggattgct gatcggtttc ttgctgatgg gagccgccat
    15241 tttcctagca aaagaatttt atagtctgat catcggtgaa agcgtgacag caagcgattt
    15301 atcaaaaatc aagttagctt ttgaacgtcc tgagatcagt cggctgatcg atataaaaac
    15361 agttcattta agtccaacgg agattttagt tgcagcaaaa gcagatatcg ttggtaacaa
    15421 agagaatgaa gcttactcgg tcatcaatga tattgaaaaa aatatgcgtc agtctcttcc
    15481 tgacaagaag atgtatatat acattgaaac agataaatat gacccaaatt attctcgtaa
    15541 ataaaacgtt tattcatgaa ttgaagcaaa aatgagaaaa atataatctt gtttttaaat
    15601 aaaacggcta caatttagcg aatttatgtt acaatgagtc cgctgtttat ttgttgtaat
    15661 taaaggagat attttaatga acaaaaagaa aagtgcgcca tggcttcaag ctttgcatgc
    15721 agtgatgcca ctttgtataa gctatatacc agtaggatta gcttgcggag tattgttgca
    15781 aaaagttgga tttgatcctc tcttatcagg actgttatcg attctggtct tttccggtgg
    15841 cgcgcagttt ttagttgcat ctatgcttac gacgcaagca tcatttgcaa cgacattatt
    15901 gatggttttc tttttagaat tacgctatac attattagga gcaagtcttg ccggttttat
    15961 gaaaaaagaa aagcggacgt ttttggctgt cttttctcaa tcactgaatg atgaaaacta
    16021 tgcggtgaac tacttaaagt tttcaactga cccttcctgg aataaaagaa aggctttata
    16081 tgtcaattgg ttctcaatga ctgcatgggc cggtagcaat atgatcggta attttttcgg
    16141 ctcggtgatt cgagtagatg ctgaccttgt ccattttgct ttgacggcca tgttcatttt
    16201 catgtttatc atgcagatga aaagcgcact actgatcttt acaggtttat tctcaggcgt
    16261 attaggggtc gtattcatgg tattattcca aaatactttc ggattgattg cagcaacagt
    16321 cattgcttca tttgccggat ttatgctgga gaaggcattc aaaaaagaaa aagctaagaa
    16381 actaagagac cgcggtaaag atgtcaatga gccgctcttc aacttcccgg aacaggaggc
    16441 caatcaccat gactaaacta tatttgctac ttttagttgc ttgtctgttc atcgtggcat
    16501 atatcccgcg cttgttcccg atgctttatt ttactcatcg aaaagtgcca tcctggttca
    16561 gtgactggat gaaatatgta ccggtggcat tatttgcggc gttagctttt aaagatgttt
    16621 tcattactca tgaacatctg gatattgctt ggaatatcaa aatagctgca atgattctag
    16681 tagcaggcgt ggcttacaaa acacgttcga tggcgttgtc agttttgaca ggacttgcgt
    16741 ctgtcttttt gctgtctatg ctttaaactg ttttatttta ttaaaaacag agattgcgac
    16801 ataagttgtt caaactgatc tatccgaact gcatttgcaa cagtcaattc tggatgcaag
    16861 tttattcgta gggataaaaa agattagtgt tggcttatga tgcaagctct tttttatttt
    16921 aggaattaga gagggatttg tccttctatg attttttgct ataataggga agatgtttat
    16981 tttatgaaag aatagttgag ggaatttaaa tgaataaaaa attgatgcaa ggaacatttt
    17041 ggctaacctt tgctaatctt ctatgtaaag ttttaggggt agtctatttg attccctggc
    17101 taagtatgat ggggaacaat caagatggta tgcttgctac gacattgtat aatgttggct
    17161 atcttcctta tggattgttc ctgatgcttg gtacagtcgg atttccaaat gcgattgcaa
    17221 aaaaagtagc tgtagccaca aaagaaggag ataacgctgc ttgtcggctg atttttagaa
    17281 gtacgatcaa tatcatgttt gtcatcggta tcgcctctgc tgcactgatg tatttatttg
    17341 ctcctttact agccggaatc agtccgattg ctaacgttaa taatgggata ctagctatca
    17401 gaagtttatg cccatctttg attgctatcc caattcttag tgcaatgaga ggatatttcc
    17461 aaggaaaaaa tgatctgcgg ccttatggaa cttctttgat tatcgaacag gctgtgcgag
    17521 tgatcgttat cttggctggt acttattact tgcgcgtatt gactgacgga accatattag
    17581 aagctgtatt gatcagtaca gtcgcttctt tcttcggtgg gttagctgcg attatccata
    17641 tgtatatcgt cggacgcaga aaagacttct tcgaacttaa agattttttg atttccagcc
    17701 gttatttcga aagagaaaac cgatctgcct ctgtctctat cattcgggaa acactaccgt
    17761 ttatttttgt cggctcagtc attactatcg ttcaaatgat cgaccaagta acgatgaaac
    17821 cactgcttca ctttttccgt ccagagatcg cagatcagca actggaattc ttattcagcc
    17881 gtgcttctgt caatccaaat aaattgacat tgatcctgat ctccatggta ggaacagtag
    17941 ctataagcag cttgccaata ttaagcacga tgaagaaaaa agatcggata caaatcgaaa
    18001 aaacagtagg agatagtttt tcgattgcgt tattgattct tcttccttca ttgactggaa
    18061 tgtctttatt agctggacca ctatacacat tgttttttgg ctatgatcct gaaagtgtcg
    18121 gatatttcca aatggctttg ctagcttctt tgtttttctc tttattcacg atcctatcta
    18181 cgatgcttca atctttgaat caccatcgtt ttgcgatccg attaactgta gaagcaatta
    18241 tcctgaaagt catcttccaa gtaatcggat taggattagt tggcggatac gggatgagct
    18301 tatcaaatgg cttagcattc ggtattgtct ttgttcgcgg ctatcacttt atgtgcaaag
    18361 aataccgaat agcaccttta gcaaagatca gcaatttttt cttaaaaact ttccgctcca
    18421 ccctgattat gcttgcagta tgttttgccg ttttcttttt gctgaatcag cgattgtcaa
    18481 tggaatcaaa aagctatgca atgatttatt gtgtggctgt aggaagtatt ggcggcttag
    18541 tatttttatt ttctcaattc ggaaaaaatg ggtttcgaat gttgaaaaat gttaaacatc
    18601 atagcagaag caaagaatag gttaactaat cgttttttgc tgtcacttga aaaacggcaa
    18661 ctcgtatgat tacgcttgtc gtttttctat tttccaattt tagtttcata tctaatctta
    18721 cttatgatat agttataagg tctggaaaaa ttctttgaaa cgaggaaatt aaacacatga
    18781 aacttgcagt agttacagat agttcggcat atctgccgga acgtatcaag aaccatcccg
    18841 acttattcgt tattccaatc cctttgatta tggatgggaa aatttataac gaaggaatcg
    18901 atatcgaagc ggatgaatat tatggacttt taaaaaacag taaagatttt cctactactt
    18961 cacaacctgt cgtaggggaa gttttgggtt tatatgagga attgaaagag aaagggtatg
    19021 acacgatttt aagtatccat ctttcttcag ggatttctgg ctttgtcaac acattatttg
    19081 cgatgaaaga tgacatcagc ggagtaacgg tcattcctta tgactctaag atcactagta
    19141 tgccgatggg acacatggta gaagctgcct tagacttaaa tgatgaaggt cagtcccttg
    19201 atgaaatact tgcacatatc gatcgaatcc gagataatac ctacgcttat ctgattgtag
    19261 atgatttgaa caatttagta cgaggaggac gtttgacaaa tggtgcagcg ctgattggca
    19321 gcttattaaa aatcaaaccg atcctgacat tttctgaagg gaaaattgtt ttattcgaaa
    19381 aaatccgttc aagtaaaaaa gcatttgctc gagctgaaga aattattggt agaagaaata
    19441 acgaaataca accgccagtc aaactttacg tgatacatgc aaataattta gaagtagcga
    19501 ttgcggagaa agagaaattg cagaaaaaat atccaaatgc agaaatcgaa attggtcatt
    19561 tcggaccagt cattggtaca catttaggtg aaaaagccat cggcattgcc atttcagcgc
    19621 aataattcct tttattcaaa aaacatacca aaaatattcc aacatcatct tggactttct
    19681 acatgttact aatgagcgtg taaaagaacg agatggtgtt tttatttcta tcaatgaagc
    19741 tctttttctt tgcaatcctc acttcaatcc tctataatgt cgcatatagt ttagtaaatg
    19801 agtaaactac taatttactt atttactaaa aataaaagga tgaggagtta aaataatgga
    19861 aataccaaca aatttaaaac ataaaccagt aatcgttgta gaagactatg ataaagttga
    19921 tggaagaaat gcattaaata cggatgcaaa agggctttca ctgggccttg ctcagtggaa
    19981 tgatcgtgga aaagtcgata tctcaggcaa ggtatggcga catactggag aaaaatggtc
    20041 acgacagtcg gaagaattac caatcacacg tattttagat ttagcgatct tagctgcaca
    20101 aggaagttta tacttccaag atgcttatcg ttatgaaaaa ttttatgatc cagaaaatcc
    20161 agtcgtagac atcattggat tacaagggaa ccgaatgacg gtagaagtag ataccaaaaa
    20221 tccaaagatc gatgaagaca tcattttgta ttacgataca ctacaaaaag acggagaact
    20281 gattggggaa cgattccgta ttttaaaacg tcttttagat gagctaggat attaatagac
    20341 agagatgatc aaaaggggaa gagaagcatg gagaatttct cactaacaga aaaccgaacc
    20401 ttttcacggc tagaaaagca aatgatctgg aagaaaccga aaagccatcg gacaagtgca
    20461 gaagaatggc ggatcgcctc cgaaatcaaa gggaattggc aagatcctga aatgaaaatc
    20521 agtaacgtac ttttggaagg agatgccggg tcggggaaga cacaattagc caaagcacta
    20581 tctgctgatc tgcaacttcc ctatacaaaa gtgacctgtt ttgcagatat ggacaaatca
    20641 gatgttttcg gagccttatt acctgtagta gattctaata aggaacaaga ccaagaatta
    20701 ctagaagcta tttaccaaac agatacactt gaagctgtac tgacactgat ccagaaccat
    20761 tacggcattg atcctttttc tgctaaagaa aaattagcag agctagttca aaggatcgaa
    20821 gctgattatc cagatgcaat ccattacagc tattaccctt ctgaaatcgt tcgagcgatt
    20881 gaaaaaggat atttattaga gattcaagag ccgactgtca tccgagatgc ctctgttctt
    20941 gtggcactta actcagcgct cgaaccaaat gggatgttga atttaccgac aggaatcatt
    21001 cgtcgccatc cagattgcgt cattgtgatc actacaaatc gaaattacca aggaaatcgc
    21061 ccattaaatg aatctttaag agatcggatg caacatgcgg aaaaaatgga tcttcctgcc
    21121 ttagaagtca tggcagaacg tgcgatggcg aaaactatga tccaaaaaca agaacttctc
    21181 ttaaagatgg cagagattat ccgtttgttg gatgaaactg caaaagctaa cgccatcaaa
    21241 ggtgtagcag gaatgcgttc ttatttttat tgggcaaata cctataagca aggacaagat
    21301 ttactccagt cgatttatcc aaaagttttg tataaattat ccactgattc agatgaattg
    21361 gcaattcttg aacaagcttt gtctgacagt ggattgctac tggaacttca ggacatattg
    21421 agaaaagaaa aatggggtca tcctcaatct gagcatacaa aaggaagaac cattagcgcc
    21481 gaagaagcaa atgaacgaaa catcactgaa aatgctgaag aaattctgcg cttagatgaa
    21541 aagaaatcgt tgtctgctga agaaccaaat gagggagaag agaaacaaga agaaacaata
    21601 gaaaaaaacg agattcctcc tcaaatgcaa aaagaacgca gtagcgatat cgaaggaaaa
    21661 aatcaaaaag aacaacaaac aggagatgat tctcaagaaa tgtctgcttc tgacatggaa
    21721 gcacatgata aggaaatcaa gaagcaactg aataaagaag cgcgacaaat catgaaggaa
    21781 acgattcatg aaaaagaagg tctcatcgtt caccgaccag ctttccaagg aaaaacgccg
    21841 gaagcacgtg agctacacat gcaagtaatg ccaatagtag aatcacttag ccggcaaatt
    21901 ttagagttac tggataatga acaaacagag acgtaccaaa aaggaaaata cgaaggtcag
    21961 cggttcaatg caagcaaagt agcatatggt gatttgagaa attttgacaa aaagaacccg
    22021 ccacatgagc aaccttcttt agcggtagct gtacggatcg atgaatctgg gtcaatgata
    22081 cgggatgatc ggatcgaagc ggcaaaaaaa gcagcgattg ccattgccga attttctaag
    22141 aaagtcgata tcccattgtt gatttacgga gatacagcag accgttcgtc aagagaaaag
    22201 acttctttgt tttcttataa agaatttgaa gacggatttc aatggttaaa tgaaaaatta
    22261 gtcactatga aaccaaggca aaacaatcgt gatggtgctg cacttcgttt agcagcagaa
    22321 aaattaaaca gacaatccgc aacaaccaaa ctgttgctta atattagtga cggtcagcca
    22381 aaagcgttgc cggattatac aggcaaaaaa gcaaaagaag atatccagtc tgttttgaaa
    22441 gaatatgaac gtcaaggaat cctatttgtc tccgcagcta ttggccaaga caaagaagaa
    22501 atcaagtcga tctacggtga aagtcgtttt gtagatatta ctgacttgaa tgaatttccc
    22561 aagcaaatga ttcaactgat tgcccgctat ctatgataaa ataaagctca acgaacgcac
    22621 ttagcagatt cgttgagctt ttcgtttcta gttaccggta acaaggatac ttctaaaata
    22681 gaatcggtta taatcaagag ggaccgttaa aggagtgaaa cgatatgata caagaacaaa
    22741 aatggtggaa agaagcagta ggctatcaaa tctatcctgc tagtttcaaa gatagcaatg
    22801 gcgatggtca aggagacatc aatgggatcc gtgaaaaact taattatttg aaagatcttg
    22861 gtatcggctt tatctggatc aatccgatct atcaatctcc atttgtcgat aatggttatg
    22921 acgtaagtga ctatcaagat atagaagaga gattcggaac gatggaagat tttgatttat
    22981 tgctaaaaga agcacatgaa ttagggatca aagtgattat ggatcttgtc atcaaccata
    23041 gttcggatca gcatcaatgg tttgaagaat cgaaaaaatc taaagaaagc ccgtatcgcg
    23101 attattacat ttgggtcgac ggggtagatg gcaaagaacc gaataattgg acatcgattt
    23161 tcggcggttc tgcttgggag tattctcatg aaactggaca gtattatctg catgtttttg
    23221 caaaagaaca accagactta aattgggaaa gcgagaaatt gaaagaagaa ctattcaata
    23281 tgatccgatg gtggttggat aaagggatag atggatttcg tttagatgcg atttctcatg
    23341 taaaaaaaga tgaatattca gtaaaagcca cagagaaccc tttctcaccc tttcaaaatg
    23401 tctcaggaat cgaagaacat ttgaccgatt tgaaacatgt ttttgaagag tacgatatca
    23461 tgactgtcgg agaagcaagt ggtgtcactg cagaagaagg accacaatgg gtagggaaag
    23521 acggttattt cgatatgata ttcgaatttg atcatatcca tatctggcag caagaaaaag
    23581 aagggcaatt agatgtcctt aaattgaaac ttgcattaag cgcttggcaa acttcattgg
    23641 atggtatcgg atggaacgct ttatatatgg aaaaccatga tgttcctcgt gccgtttcag
    23701 tgtttggaga tactcgtccc gatttttggg ctatgtctgc aaaagcaatc gctatgatgt
    23761 atttcttttt gcaaggtacg ccatttattt atcaaggaca ggaaatcgga atgacgaata
    23821 tgccctttga atcgatcgat caagttgatg cagtagattc gaaaagactt tataaaaggc
    23881 tgcttgcgga aggtaagaca agagaagaag cattggatat cattcgtgaa acaacgagag
    23941 acaacagcag gacacccatg caatggactt cggaacaata tgctggtttt tctacacatg
    24001 aaccttggct gatcactaat ccaaatacca aaacgatcaa tgtagaacaa caagaatatg
    24061 aaccagagtc ggtcctgcaa ttctataaga acatgatccg cattagacaa accaataaag
    24121 gtctgatata cggttcttat aaagaatatc ttcatgaaca tccacaactt tatgtctatg
    24181 aaagatattt agaagatgaa caatatctta ttatggtaaa cttaacagaa tcacttgctg
    24241 actatgaact tccaaaagaa gcagatcagt cctggacatt actcttgtca aattcctcta
    24301 gcggagaatt tgaagctaaa ggcatactag caccttatga agctcggtta tataaaacaa
    24361 acaagtaaaa cttcctcgtt aaagttcatt agttggactt tgcgaggaag tttttcagat
    24421 tgctctgtca attactcatt tcaataattc gctctaggac caccgatata tttaggacga
    24481 gagcgaaggg ctgtcacgtg agcttgtcca agggttttca aacgaggtct gacaggtact
    24541 tggacatgtt tgatatgcat accgattgat gtgtcaccga tatcgatacc ggcttgtcca
    24601 actacgtgtt ccacttcgac aggatcattg aactggtcaa aagcagcaac agaacaagca
    24661 cctccagcat gtagtgcagg aacaacagag acaatttcga aattttttgc ttctgccact
    24721 gttcgttcta ctactaatgc tcggttcaaa tgttcacaac cttggacagc tagcgcaatt
    24781 tctttaggat ccaacaactc ttttaacgta cggatgatcc attgaccaac ttccgcactg
    24841 gaattttttc caatatgtcc tccaacgact tcgcttgtgc tgcatcctaa aacaaaaaga
    24901 tcaccttttt tcaagtgtgc ctcagccaat atgtcattaa ctatttcagt caattggtct
    24961 tttaattctt tttctgacaa cttcattttc taaaaccttc tttttcaatt aatattgctt
    25021 ccgtgcttga atacgttgaa gacttgcagt tataggaaag gcaagtccca ttccaatcag
    25081 attttggaag atgtttcctg gtatagaagc cactccagcc ggccaatcat ataaaatcca
    25141 accggcgata ccatagccga tgatcataac taagcttcct aataaaagcc ctaatcccca
    25201 catacgagta gaagatttgc tgaaagaacc agcaacatat ccttgtaacc cgtggatcac
    25261 taatgaaaaa actgcccact gcggatatcc tgaaatcaaa tcgatcaata gaccactgga
    25321 agcaccaaca actaatccac ccgttggacc ataaagaaaa gcagcggtat agatcccggc
    25381 ctcgcataat gtcacaaatc cattcgtagc cggtacaggg atcaaaattg ttaatgaaag
    25441 ggcaactgtc aacgcaatca gcatagcata gatcgttaat tgcttagttt tcatttctca
    25501 ttcctccttt ttgaataccg ccagaccaga cacttccggt atgatcgttc atcagcacgc
    25561 cattagaaat acaatgggca acaaattttt tactttctgc tactgctttg gtaaaatctg
    25621 cccctaacac aagattcgct gttattgcag aagaaaaggc acaaccggcc ccgtttactg
    25681 ttgtagtgtc gattttttta tgagaaaata cctcataatg atcaattgaa tagagaagat
    25741 ccacagcttc atctccagcc atccttgtgc cgcctttgat cacaatgttt tttgttcctg
    25801 aagcagtgag ttctttagcg agatggatca tttctgttaa cgaagaggga agagcttgac
    25861 cagaaagtaa agcagcttct gccaaattag gcgtaatcat cgtaacaagt ggaaaaagtt
    25921 cttcgatgag ctgcgaaata tacaattgat tgatcacagc ttcagtttct ttgaatgcca
    25981 aaacagggtc taaaatgatt gggatatttt gttttgtttt tatgaagtct cgaacaacca
    26041 tgattgcttc acttgaattc aataacccaa ttttgatacc atctaatgaa gtcattttat
    26101 caatcgaaac taactgtcgt ttaagcaatt ccaaagatat agattccagc ataaatcgct
    26161 cattaatatc tagtgcccct atagaagtca aggcgcttag tccgaataga ccaaaattct
    26221 caaaagtttt taaatctgtt tggattcctc caccagcaaa tggatcagaa ccgccaattg
    26281 tcaagattct tttcataaca ttttcctcca tgaataagag tataacgaaa ttttcaaaaa
    26341 agaaaaacag ccaattggca tatttttatc tcatccaatt taaaaaaaca aaaagaaaag
    26401 aaagaccaaa atggatcttt ctttccttaa atagttagga cttttgtgat tttaattctt
    26461 tctcgatttg aagccgttgc actttcattg tagctgttcg cgggattgca tcaaaatcac
    26521 ggatgatagg gacgttcaag cgaggaagtt catggacttg ctcccaccat cgattccaat
    26581 ccatttcttt atcaggagct aaagcaataa tcggttgtgg agaatgattt ttatccctta
    26641 caatcactac ttctgccaaa aaatctaacg aatctaataa aaagtcttca atagctaatg
    26701 tactattgat cgtttcgatc aaatccactt gccgatcttt caaaaatagg tggccatgct
    26761 tatccataaa accgtaatct ccgctatccc accaatcgcc ataaacattt tcctggaacc
    26821 gtgcatcttc cttgtaataa gtcaaagccc gtcctttaga cagtaattgt atatgaccat
    26881 ctgtgttttc aggtaggaca ttcccaactg aatctgtaat ccgagcgctt gtcatgtctt
    26941 ccagtccaac gcccatatct cttgcatcac ttgttttcaa actctctagt gtatgagcct
    27001 taagaatcat tggtccgcat tcactttggc catatacttg aagaaatacc gcatcctgtg
    27061 ccatcgacgt tcgcaaaaat gtcttcattg tctggttgtt tatagcatca aaagtggagt
    27121 gatagtatcg gatacccgaa aaagcttctg gatgctcctt agcagtaaaa cgccactgaa
    27181 caaagttatt cggatgggtc tctagtgcaa ttggccggtg agcttccaac atatgaacaa
    27241 cttttgaact ttgtgcgttt gctaaaggca tcataggaaa tcccatggac atcaaagaag
    27301 agataccaat attgaatctg gaatgcactg gagagatgtg aaaagcgatt aatttctttt
    27361 ccgaaatttt tgtgaaaact gttttttgcc atttcgtccg ccatcccata gaatgagcag
    27421 aatgacaaat aagtttaggg ataccagttg taccagaagt atgggtcata taagaaattt
    27481 catttttgtc taaaaatgat tgagaaacag acaaagcagg ttgttccatt aaataaagaa
    27541 taggaa
//
LOCUS       DADVXU010000030        26561 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.032, whole genome shotgun sequence.
ACCESSION   DADVXU010000030 DADVXU010000000
VERSION     DADVXU010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 26561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.032"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(19..729)
                     /locus_tag="KB953_001185"
     CDS             complement(19..729)
                     /locus_tag="KB953_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HBB6866173.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(726..2198)
                     /locus_tag="KB953_001186"
     CDS             complement(726..2198)
                     /locus_tag="KB953_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBB6866174.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            2336..2809
                     /locus_tag="KB953_001187"
     CDS             2336..2809
                     /locus_tag="KB953_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBB6866175.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(2962..3513)
                     /locus_tag="KB953_001188"
     CDS             complement(2962..3513)
                     /locus_tag="KB953_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBB6866176.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            3618..4148
                     /locus_tag="KB953_001189"
     CDS             3618..4148
                     /locus_tag="KB953_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HBB6866177.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(4631..5074)
                     /locus_tag="KB953_001190"
     CDS             complement(4631..5074)
                     /locus_tag="KB953_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB6866178.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(5090..6250)
                     /locus_tag="KB953_001191"
     CDS             complement(5090..6250)
                     /locus_tag="KB953_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBB6866179.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            6757..8457
                     /locus_tag="KB953_001192"
     CDS             6757..8457
                     /locus_tag="KB953_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866180.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(8559..9392)
                     /locus_tag="KB953_001193"
     CDS             complement(8559..9392)
                     /locus_tag="KB953_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBB6866181.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(9696..11861)
                     /locus_tag="KB953_001194"
     CDS             complement(9696..11861)
                     /locus_tag="KB953_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBB6866182.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            12121..14415
                     /locus_tag="KB953_001195"
     CDS             12121..14415
                     /locus_tag="KB953_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBB6866183.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            14408..15073
                     /gene="pgmB"
                     /locus_tag="KB953_001196"
     CDS             14408..15073
                     /gene="pgmB"
                     /locus_tag="KB953_001196"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBB6866184.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            15066..16073
                     /locus_tag="KB953_001197"
     CDS             15066..16073
                     /locus_tag="KB953_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBB6866185.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            16095..17114
                     /locus_tag="KB953_001198"
     CDS             16095..17114
                     /locus_tag="KB953_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBB6866186.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(17395..18762)
                     /gene="nhaC"
                     /locus_tag="KB953_001199"
     CDS             complement(17395..18762)
                     /gene="nhaC"
                     /locus_tag="KB953_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBB6866187.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(18871..20292)
                     /locus_tag="KB953_001200"
     CDS             complement(18871..20292)
                     /locus_tag="KB953_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBB6866188.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(20535..22412)
                     /gene="tdc"
                     /locus_tag="KB953_001201"
     CDS             complement(20535..22412)
                     /gene="tdc"
                     /locus_tag="KB953_001201"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HBB6866189.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(22703..23959)
                     /locus_tag="KB953_001202"
     CDS             complement(22703..23959)
                     /locus_tag="KB953_001202"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBB6866190.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(24071..24276)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            24539..25840
                     /locus_tag="KB953_001203"
     CDS             24539..25840
                     /locus_tag="KB953_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBB6866191.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(25909..26508)
                     /locus_tag="KB953_001204"
     CDS             complement(25909..26508)
                     /locus_tag="KB953_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBB6866192.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT         8061 a         5473 c         4758 g         8269 t
ORIGIN      
        1 tacatggtgt tcaaaatatt attcgccttt tgcttctttc actaattttg cgaatgcttg
       61 ttgttggaat tcagcagcta aggctttcgt acgttgttct tgctctgctt tgctcatttt
      121 tggattttca gtcattgctt ttaccatacc gtcttgaaca taagcttttg cttcactttc
      181 catcgtactt tgctgtttct tcactaattc agctaacttg gctgcttggt ccgtacttaa
      241 ttctacccat gtctctggga ttttgaatgt attcactcgg ctgtcatatt cgtgattgtt
      301 atcagcaata ttgaaccaga atttatacat gtcgtttttg tattcccaat aagtagtttt
      361 cgtcactttt tcagcatcgc cgtctgtctt ttctacttta tttgtttcgt tatccgtacc
      421 agttgctttt ggtttttctt gtttttcatt tgttcggtaa agatacactt tttcttttcc
      481 attccctaaa gctttataca aaagcatatc tgctccttgg gaatcagcac tggaaacaat
      541 tttttctgtt tttgtttccg ttactttttc cattccaaag tgattggaca aattagcgac
      601 gatcaaactt agtgaagcga cgaagattag tccgaaaatc aaagataaaa aggtttgcca
      661 tgttttttta gcgaacacgt tcgttaaagc aaaagcaagc aagctgacaa tcaaaactcc
      721 aataatcatg cattatcccc cgctttcatt agaacaggtt cttttttctt caataaaaag
      781 gcaataataa agccaagaat cccaaagatg atcgctacga taaacgcggc atggtaacct
      841 gacagtgtgg cattgattgc ctgatcttta taagctaatg gcgtattatc caatacagat
      901 ttgcttggga tatttgcttt tgtcacattc gtcaacacac tgatcaaaat agctgtacca
      961 atcgagcttg cgatttgacg gaatgtatta tttaccgctg tcccatgact gatcaagtta
     1021 tttggcaatg cattcatacc agaagtcgtt accggcatca tgaccatgga gatcccaaac
     1081 atacggatcg cataaaggat cacgatatat aaaactggag tcgtttttgt caagaaagca
     1141 aatggtgcag ttgccgctgt caaaatgaac atccctgtga tggctaaacg tttcgcgccg
     1201 tatttatcga aaatcgcacc cgtcacaggc atcatagccc ccatgatcaa tgcacccggc
     1261 aacaacatca agccagaatg gaatgctgat tctccacgaa tattttgaat atacaatggt
     1321 acgaccattt ctgcccctac catcgccata tttgttacac cagctaaaac agctgcaatc
     1381 gtgaatgtag atgatttgaa tacacgaagt tccaagaatg gatgttccaa atgtaattga
     1441 cgccatgaga acaaggcgat gaaaatcaca ccaacaacta agaaaccaat tactttcaag
     1501 cttccccagc cgtcatctcc tacactagaa aatccgtaaa gtaaactacc gaatccaatc
     1561 gttgataaaa tagcggataa aatatcaatg ctaggatttg acaatggaat aacacttttc
     1621 attaggaaga atgctaaaac caagacaaga acaacgattg gcagtaccat accaaacaag
     1681 tcacgccaat ggtagttatc aatgatataa ccagataatg tagggcctaa agcaggagca
     1741 agtccaataa cgatccctga catccccatt gctgatcctc ttttttctgg cgggaaaata
     1801 gatagcatga tattttgcaa taatggcatc gatactccca caccgcaagc ttggatcaaa
     1861 cgaccgatta gtaatgtact aaaattaggt gctgtaaagc aagtaatcgt tccgatcaaa
     1921 aagatcgtca tggcagaaag atataaattt cttgaactga atttattgat cagccatgct
     1981 gttattggga tcatgatccc gttgactagc aagaaaccag tcgttaacca ttgcacatca
     2041 gacgctgaaa tatcgaaagc tttcatcaat gtaggaaaag ctgtcgtcaa taaggtctga
     2101 ttcaaaacgg tacaaaatgt accaattaat aagacaacaa ctaaaagtgt ccgattatat
     2161 ggttttccgt aaatatctac gggttggttg ttactcatat ttctccttct ttctgaattt
     2221 tttctgaaac tactagaata ctttagtcct aaattcgcta tttgtcaacc tacttgacac
     2281 ataagttaat acggatataa ttaagggaag caagaggaca aaaggagtga caaaaatggt
     2341 tggggcttcc cttaaacgtt tgttggaaaa tgatcaattg gttgttggca tcagtgaatt
     2401 aagtgatatc gtaggtgtgt ctccacgtca attaaggtat tgggaacaaa agggattcat
     2461 tcgttcgatc gaagctgatg cgaattgtcc tagaaaatat cgtttaccta cagtcataaa
     2521 agtagaactg atcaaaaaat atttagatga cggatttact ttgtcaaaag ctgcagaaaa
     2581 ggcagaaatc cgccaaaaga aaatgcacca tgtccgtaaa gttttttcaa aagcaatcaa
     2641 agatatcgaa ttgatcgacg aacgctatac gattttacaa atcggtaact tcaaagaaga
     2701 gaatgctaag atgtacatca ttcatgacaa cgagacagac gaattaactt acaagctcct
     2761 tcctgtcgat gaaaaagctg actacgaaca gttgttaaaa gaactctaaa cgccactaat
     2821 ctcctaagta ccaagctttt taataagtag atgctacata aatacttgaa aaaaggcctg
     2881 tttgaacata agaatatccg aacggtgatc tgcgtttctt tcatatagaa aggaattctc
     2941 catcactgtt cggatatttt tttatttttt tcggttactt tcttctaata catctgttag
     3001 ccaatttgca tattctgttt cccgattgat cgcatgcgct aaaatcaggt agtgtccgta
     3061 attttcatgt ttttcttttt cagtaggaaa gatcgttttt tttcgctcta atagatgaag
     3121 tcgtttttct tcatgaaggt cacgctgctg ctgaataatt tctactaatg ccggatcatt
     3181 cacgtctttg acgaaataca atttcagaac aaattcgtct cgattaaccg gtagttcatt
     3241 ggtgggtgta tgaatccact ctgccaattg ttccttccct tcttctgtta attcataaac
     3301 tttcttttcc aatttttcac ctgtgatctc tacttgatgc tgaatgattc cttgctcttc
     3361 catttttgca agttcaggat aaatctgact atgttttgct tgccaaaatt ctcctatttc
     3421 atttttgaac gattgattca attcatatcc tgtcatcttc ttcttactta ataaccctaa
     3481 caaaatatat cgaagtgtat tttttcttgc catcagtttt ccctccttga caaatcttta
     3541 aatccattct atcataaatg agtaatcgaa aacatgtagt ttattacatg ttttaattta
     3601 taaggagatg cttaaacatg aaagaattta aagaattgga agattttatt ggaactcact
     3661 ttatctatac gtatgacaat ggttgggaat atgaatggta tgccaaaaat gaccatactg
     3721 ttgattaccg tatccatggc ggaatggtca aaggccgttg ggtaaaagac caagaagctg
     3781 atatcgttaa attgacggac ggcgtattca aaatcacttg gacagagcca acagggacag
     3841 acgtggcatt ggactttatg ccaaatgaaa agaaacttca cggaatgatc tttttcccta
     3901 aatgggttga agaacatcca gaaatcactg ttactttcca aaacgaacat atcgcagaaa
     3961 tggaagcagc tcgtgaaaaa tatgaaactt atccaaaatt acttgttcct gaatttgcta
     4021 cgatcactta tatgggtgat gcaggtatag acaatgaaga tgttattagc gaagaaccat
     4081 acgatggaat gccagatgat atccgtagcg gaaaatactt cgatgaaaat tataaacgag
     4141 taaaataagg ttgtgaaaga gaggttgtga aatcagcgtt ctgacctgag tattagagaa
     4201 agaaacggaa aaatagttcc tcatgttttg ccgttttttg agctaatacc gagggtcagc
     4261 tgattgaaca ccgtttatca agaggttgtg aaatcagcgt tatgctttga gtaataagaa
     4321 cgctagttag aaaatagctt ttcatatttt ctaggctagt gggcttatta ccgagaagca
     4381 gccgtttgaa caccgtttat caagaggttg tgaaatcagc gttttgacct gagtattaga
     4441 gaaacaaatg gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgagggtc
     4501 agctgattga acaccgttta tcaaagattg taacaactgc atattcacac agttaaatta
     4561 gtgacttcaa aatgaaataa tgctcccagc aaaaaattag gctgggagca ttatttcgtt
     4621 attctgttca tcaaaaatca gcgttttgat tccatttcat cacaagttcc tcctcattta
     4681 ctgcttcatc tttttgctgt gtttgaattg taaaaccttg tgaaagatag aaatgaatag
     4741 ctgattgatt tttttgatag actgtcaaag tcagtgtttt attggtttct tttatttttt
     4801 tcattagttg cgtaccgatt ctttgttgcc ggtattccct tttcacaaag attcctgcga
     4861 taaactgatc aatcacccca gcaaaaccaa taatttcttg atctatttct gctaagtaaa
     4921 tcactgcatc tggtaaagct ttcctaacag attctacttg attctcccaa taagttgatg
     4981 gaatgaattc atgagaatcc aagttagcat gcagccaaat cgatagaaca gattccagtt
     5041 ctttggcaga caattttgtt gttgtgcgga tcattttttc tctccttact tattcattga
     5101 ttttaaaata aaaaccattt ttttgaagct cttttacaag ttttcgcccc tcaaaaaagt
     5161 atatcttctc ttgttttact tttctaatac caaaagaaac agcaacttta ggatcatcag
     5221 ctgaaacgat tcgataagtt ccaccaggca attgatccag cttgactttc ccatgatggt
     5281 cgctttgtac tgaaaacggc acaccatttc tagtaagtgt ttttttctta tgtttcaaaa
     5341 tgaatgaact accatttacc ggtttccctg acgaatcact aataataaat gaaaaatcaa
     5401 gcactttttt acttaatcta tactgataga ttagtcgtcc gataaacact aacagtatga
     5461 ctacagctat tatcgttcct gcaagaataa accattgccg catggttctt tcctgatttc
     5521 cttttccttc ttcttttttt accgattcgt tataaggaat ccgatgtcct gtcactagca
     5581 aacgatgcgt gttgatcata tacggcgtac atgtaagcaa ggtaactaag tcttctcctt
     5641 ctgaaatcgt tagaacagac gtgtcatctg gcagtaccac ttgtatattt ttaacttgat
     5701 aagccaattt ctcccccaaa acagtcaaaa taaacgtgtc tccgttttct aaacgatcta
     5761 attcggtaaa taaccgtcga ttaggtaaac cgctgtgagc agagatcact gaatgcgtcg
     5821 aatttccacc aagcgggaaa gaggttcctt gcaagacgcc tgctccattt tcaaggatcc
     5881 taccattgag ccgatcataa agtgaaatat ttaattggat cttaggtata ttgatacttc
     5941 cgatcagcca ttcctctaat tggctggatg ttgtactttc atttctgttc ttttcattga
     6001 aaggatcagt accaggattg aatccttggt cagttaatcg ttggtttttc tcctccattt
     6061 tctttcgctg cacttcaatt tcagctgcgg tccgtttttg tacttgttcg atacgttttt
     6121 gatcaataaa actatttaaa gaatccacat aaaatggata taaagcgatc agtaacccta
     6181 cgacgataca tattaataaa aacaaacgaa acaaaagtcg acgagactgt ttttgcttac
     6241 tctgcttcac atgtgtcctc ccttgcaagt ttttttgttc tttcttttat tatatgggtt
     6301 caggaaaaaa atagaaaatg aaagccgatg aggttgtgaa aaagctgtcc tgcatcaagt
     6361 aataagtaca gcccaaaaaa atagctactc atattttttg ttgggttggg cttattaccg
     6421 cagatgcaag cttttgaaca ccgtttaagg aagaggttgt gaaaactgcg ttatgctttg
     6481 agtaataaga gcgctagtca gaaaatagct tctcatattt tctaggctag tgagcttatt
     6541 accgagaagc agctgattga acaccgttta ccgagaggtc atgaaaaagc tactttgttt
     6601 cgagtattaa gaaaaattct gtttcaaaac aaggagatgc aacaaaactt atgtcgcatc
     6661 ctctctcctt agaactttca cttatgtttt tcttgtaaaa ttcataaata ttttcatgaa
     6721 ttttctaatt tcttgcctac ctctcgcata ggatatatga tgaatttatc taaagtttgc
     6781 aaaggagttc gacaattgaa aaaatatggc ccatttttag caattttttt cattgctttg
     6841 gcatttctca tgcctcattt attcacacat aaactaattt tgggtgctga tgccccattc
     6901 cattacaatc ggttctatga tgccgcgcaa caaattaaat atggggattt ccattatttt
     6961 ccttcccttt atggattcca gcaaagtggc aggatcgtga atgccgttta cggcccattt
     7021 ttcgcttatt tacaaggatt gatcgtgttg cttgcaggta gctggtttca ttatcagatt
     7081 ctttcaaatt tcattcttta tcttatttca ggattttcta tgctgatttt actgagatat
     7141 gtcaatgttc gaaattgggt aagtgtacca gtagccgttt tattcatgag tacgtattct
     7201 attcaatact ggtctatcaa ccaaggattc actagctggg ggacttgttt ttatccttta
     7261 tgtatgatcc ctattgctga tttcgtcctt cataaaaagt tccctgttat caaagtcgct
     7321 gtctcagtgg gacttatgac acagatccat ttactgtctt cgatcatgtt agctttgatg
     7381 tatatccctt tttatcttta ttactttttt aaccagactg aaaaaggaaa agcactgctt
     7441 caactgatta agtcggtctt gctttatttt gttctgacag caaatatttg ggcaggatta
     7501 gctattgttt acagtgggaa taagattctt cctccctttg ttaacagaga aatgagtcaa
     7561 aaggcgatag atctggatgg cagttattgg ctccattacc ctaaggcctt tcctatattg
     7621 atattatcag gttttttgct ttttattttc tttcaacaaa agaacacctt attccaaaaa
     7681 atcgtgttgt acacaagtct tgttttcttt tttctttcta ctagcttgat tccatggacg
     7741 acattgatcg aaaaaaatgt gccttttatt agtttgatcc agtttccatt tcgatttttc
     7801 gtgccattta cgctattgct tctactttac cttgcccttt cactgaacca gtggtctgaa
     7861 caaaagtggt tccgtctttt aagtatattt ggcattctta ttttttcagc acagacgatc
     7921 cagaacttat accagcatct ggaaaagtgg gaaaatgaaa catttgtcag tcgacatact
     7981 tatctattcg atacgccgga agaagcaagg aaaacattct tcagcaaaga tatgtctacc
     8041 agcttgttcg ttttccaaaa aaccacacct gactatctgc caatctataa ggagacaaaa
     8101 gacaataaat acgaccggta taacgaagaa atactagaaa acgaaacaac ttttataaaa
     8161 acacatccag gcggtctttt gacaatcaag tggaaaaaca ctggggacaa aactgttcat
     8221 atccctgtta tcgtctacga tcggactgtg ctacagcaaa atggaaaaac attgactgat
     8281 tatgaagtaa cagatatcgg gaccccaatc gtgaagcagc aaaaaggaat caacgagctc
     8341 acgcttcatt atcaaacgcc aatctatttc tatttcattc tttctcttac tttgattggc
     8401 tggtttacct tgctatgttt atttatttat catagataca agcttttacg tgcttaggca
     8461 aaagggataa atacatccga tcaaaaaact ctccaaacgg tataatcgtt tggagagttt
     8521 tattttaatc agttttttta tgttatatgt tatatttttt attcgttcac atctaattcg
     8581 atttccaccc cgtagtgatc actgatgatt ggactatcga tcccatcaaa aatccgttga
     8641 tattttttaa cctgcattcc ttcagggacg aaaataaaat ctatacgcaa tttttctgta
     8701 ttttgttccc aaccatctat tttcttttcc acagtagctg cttttccttt ttcattcgcg
     8761 acaacatacg aatcttgaag aagaaggcaa ctttctctta cagtataata accttgagag
     8821 tctactggat tattgaaatc acctaggaaa aaaagtggta cgcgagtttc caaaaagtag
     8881 ttttccaagt ttttccactc aaaataaaag ccactttttt tatctgtcca ccaagaaaag
     8941 tggcagctcg agacaaaaat gtcctgatca tctatctttg ttttcccaat caagatcttg
     9001 cgtgtctgat aatcttcttt cttttgaaat tgggagacgg taaggacctg actttccaat
     9061 gtttttttcg taagcagtgc atttccttct tcatatttat cataaccgat gtggctcatt
     9121 tcccaactcc aaaaatactc ttttccgtgt tcctttaaaa gctgaactaa acggtaggca
     9181 aaattatctt tacggatagg tacttgattc tttaccggac agaacttcat tagcttcgca
     9241 ggttctactt cttcagattc cagtaattgg ttgatttctt gtaaagcaat cacttcataa
     9301 tcttctaaca ggatcttatc tgctaattct tgcaattttt tctctggttc tggctcaagc
     9361 cagctatgcg tgttgagtgt catgaatttc attggttttc tccttgtctt catttcgaaa
     9421 caaaagggtt gtgacaaaat ttttgtcaca acctctccag caaatagtgt tctcctgcta
     9481 actcctcagt aataagctca atggaccagg agctattttt tgtttggctg tttttatcac
     9541 ttggtgcagg gcagcttttt cacgacctct ccactaaaca gtattcaaac tgctgcttct
     9601 cggtaataag cccaaccaaa cgaaaaatag gaggagctat ttttgtttgg ctgttcttat
     9661 gacttgatgc aggacagctt tttcacaacc tctccttaca ttttaatgga gccgatcact
     9721 ttaccagctg tcactgtacc taattgcttg atcactactt cttctacttt atcgtttgta
     9781 aatacgatca tgatcgttgg atctttttct tcgttttgga tcacatcaag atccatctca
     9841 gctaatttcg ttcctgttcc tacttcttgg ccttctttga cagcaatctc aaaagctggc
     9901 tgattcattt ggactgtatc gattcccata tggatcaaaa cttcgatacc atctttcgtt
     9961 tccagcccaa ttgcatgttt gctagggaaa atacttgtca cttttccagc gatcggcgca
    10021 acaacttctc ttgatgttgg aataacagca aagccgtcac ccatcatttt ttgtgagaat
    10081 accggatctt ttacttcaga taaaggaatc acttctccag aagctgcgga aactagttct
    10141 tgttctatac ctaagtattg cttactacct gcttgtccat ttcctgtaga aaccatttct
    10201 ggctctggaa tctctgctcc tgattgcagc agatcttcga tatctgattt caagacatct
    10261 gcttttggtc cataaactgc ttggactcca ttgtctttga cgatcaatcc catcgcccct
    10321 gctcgtttcc aggcttcttc agtccctact ttttctcgat catttacact tacgcgtaac
    10381 cgagtcatac aagcatcgac atctttgata ttttcttttc ctcccagcaa atgaatgatc
    10441 tggaccactt gctgatctgc tgttcctgtt ccgctgttag atacaacggt ttcttcactg
    10501 tcattgtcgt aatttccgtt acgtcccggt gtagcaaagt tgaatttttt gatcaagaaa
    10561 tttgctaaga aatacgtaac aacaccaaaa gcgatcacac acaacacgaa gttgataaga
    10621 tctccaccta gacccgcttt gatagctaac ggtgtccgag tcagcaattc gatattccca
    10681 aatgaatgga cccgcaatgg caagatatct gccatcgcaa atgcagcccc ttggatcact
    10741 gaataaatga catataatgg cacagcagca aacataaaca tgaactccaa tggttctgtt
    10801 acacctgtta gaaagacagc caacgctgct gaaaaataca ttgatttata tcttgctttc
    10861 ttatcaggat cgacattacg atacatcgca aaagccattc ccatcaagat acctgaagaa
    10921 ccaatcattt gtcctacttt gaaacgagcc ggcgtccagt tatgcaacac aaactcatat
    10981 tgcgacatat cccctgcacc tttaagattc actaaatcag tggcccaagc caaccataac
    11041 ggatcttgcc caaatacttg tgtccccgcc tgtgcgcctg ataaaatctc atacgatccg
    11101 ccaagctgtg tatagttgat aggaatcgtc agcatatgat gcaatccaaa tggcaatagt
    11161 aaacgttcta atgttccaaa taaaaacggt gctagaatcg gtgcgctttc ttgtgattct
    11221 gcaatccata aaccaaaatt attgattcca gcctgtacgt ttggccacac cacagcaaga
    11281 atcaatgcca cgattgttga ccataagata acaacaaacg gaacaaagcg ctttccattg
    11341 aaaaatgaca aagcatccgg caattttcta taattatagt acttattata agccattgcc
    11401 ccgacaaatc cagcgatgat ccctacgaat actcccatat tcaaagctgg tgcttccaat
    11461 acacttgtaa aaaatccttt aaccatgatc ttcgtaccga acagagtgtg ggtaaatgca
    11521 tcttcattcg caagcatgtc acttgtgaca ccaaagatag ccccagtgat ccgattgatt
    11581 aaaataaatg agatcccagc tgcaaatgct ccgcctgctc tttcttttgc ccaacttcct
    11641 cctattgcta aagcaaataa aatatgaaga ttaccaataa ttgcccaacc aatattttca
    11701 attactccgc ccgttgtgac aagcggcgtc caatcaggat tgatcaaggg aattgatttc
    11761 cctatactga tcatcaaccc agctgccggc ataactgcta ccacaaccat taaagccttt
    11821 ccaaattttt gccaaaactc aaaactaaat atctttttca ttccgtttag ctcctttacg
    11881 caatcggttt cgttaaacgt attatagcgt gcgttttctt ttttgtaaat gctttatttt
    11941 ttatttttct gtctatacca ttctataaaa cctataatac gtgtgttccc ttctataatt
    12001 ttctcctaaa aatttatttt tttaagcttt tttctatgaa atattttctt ttgtctattg
    12061 acttgtgcaa tcggttgcgt ttattatgaa cgtataaaac agctcattag gaggagaaaa
    12121 atgaaacaga tcaaacgact atttgctatt gatccatgga aaatcagcac gacacaatta
    12181 gataaaaaaa atcttcgact gcaagaatct ttaacaagta ttggaaacgg ttatatgggg
    12241 atgcgcggga actttgaaga aacttactca ggggaccacc atcaaggtac ttatttggcg
    12301 ggtgtctggt atcccgataa gacacgtgtc ggctggtgga aaaatggtta tccagaatac
    12361 tttggaaaag tgatcaacgc cttgaacttt atcgcgatag atatttcgat caacgatcag
    12421 ctgatcgact tggcttcttt ggaaccagaa gacttttact gggaattaga tatgaaaaat
    12481 ggtgtattat ctcgtacatt cacaatcact acttctacta ataaagtacg catttccttc
    12541 gaacgttttc tgagcattgt caaaaaagaa tcagcttata tctatttaac tatagaaatg
    12601 cttgaaggta gcggaacagt caaactcgta tcaaaattgg atggaaatgt tcaaaacgaa
    12661 gacagcaact atgatgaaca tttttggaat gagattgatc gtggaactat agaagaaatc
    12721 ggctatttga cgactaaaac tattccaaac aactttggga ttgaacaatt tactgttact
    12781 gcagcaatgc gccatttcgt taatggaaaa tcagtcatcc ctgactatca cgaagatact
    12841 ttagcattac atggttgctt gtctgcagaa ttatctcaag gtgagacatg gtcagtggaa
    12901 aaagaaatta tcgttcttac tagtagagat attccagaag cgcagcaagc agaaaaagct
    12961 gtctcagaat taacgaacca gacaaacaat tatatccaag caaaagaaga gcaaacagcg
    13021 gcttgggcag aacgatggaa gcttgccgat gtggtcatcg aaggagacga agaagcacaa
    13081 caaggtattc gattcaattt gttccaacta ttctctactt actacggcga agatgaacga
    13141 ttgaatatcg gccccaaagg attcacagga gaaaaatacg gcggagccac ttactgggat
    13201 acagaagcgt atgctgtccc tctttatctt gctttagcca aacctgaagt aactaagaat
    13261 ctgctgaaat atcggcataa tcaactgccg caagcaatcc acaacgctca gcaacaagga
    13321 ttaaaaggcg cgctttatcc tatggtcacc ttcactggtg tcgaatgcca taacgagtgg
    13381 gaaatcacat tcgaggagat tcaccgaaat ggcgcgattg cctatgcaat ctacaattat
    13441 acgaattaca ccggcgatac ggaatatttg aaaaatgaag gactggaagt tttagcagaa
    13501 atcgcacgct tctgggcaga ccgtgtccat ttttcaaaac gagcaaataa atacatgatc
    13561 cacggtgtga ccggcccaaa cgaatatgag aacaatatca ataacaattg gtatacaaat
    13621 acgatcgcag cttgggtctt gcgttacacg cgagaaaatt atttaactta tcaaaaagaa
    13681 gctgctatca ctatcagcga agaagaacta gccaaatgga aagatatcgc agagaacatg
    13741 tacttcccag ttgacgaaga attaggaatc tttgtccagc atgacacatt cttagataaa
    13801 gatctcatgc ctgtatccga tattcccaca agcgatcttc ctctgaatca gcattggtct
    13861 tgggacaaaa tccttagatc ttgcttcatc aagcaggcag atgtactaca ggggatttat
    13921 ttcttcaatg atacttttac aaaagaagaa aagcaaaaaa actttgattt ctatgaacca
    13981 atgactgtcc atgagtcttc cctttcgcca agtatccatt cgattttagc agcagaatta
    14041 ggaatggaaa gcaaagctgt agaaatgtac cagcgaacag ctcgcttgga tttggataat
    14101 tacaataacg acacagaaga cggcttgcac atcacttcca tgactggtag ctggttgtcg
    14161 attgtccaag gatttgctca aatgaaaact gcccatgatc agttgagatt cgcaccattt
    14221 ctgccaaaaa cttggaaagc ttattctttc catatcaatt atcgcggacg tttattgttc
    14281 atctcagtca cagatgaaca agttactttc gagttattag aaggttctcc tctagaaatt
    14341 tacgtctatg aacagtctta ttctttagcg gatcgtctag aaataacgct aagaaaggag
    14401 gaagcaaatg tttaaagggg tattgtttga tttagacggt gtcattaccg atactgctga
    14461 attccattat cgtgcttggc gaaagcttgg tcaggaaatc ggcattacga tcgaccacac
    14521 attcaacgaa cagctaaaag gcgtcagccg agaagactca ctctctttgc ttttagctta
    14581 tggcggaaaa gaacactctt tttcaaagga agaatttgca gaattagcga aaagaaaaaa
    14641 cgattattat ttagagatga tccagacgat cgaaccaaaa gatgtcttcc ccggggtcgt
    14701 gcctttactg gattctttaa aagaagaaaa aatcaagatt gctcttgctt ctgcaagtaa
    14761 aaacgggcca ttcctacttg aaaaaatggg tcttacacct tactttgatg ctattgccga
    14821 cccggcagaa gcaacaaacg gaaaacctgc gccagatatc ttcctccttg cagcaaaagc
    14881 tgtcggacta aaagcagaag aatgtatcgg gatcgaagac gcgcaagccg gtatccaagc
    14941 catcctctca agtggcgctc aaccaattgg cgtgggacgt gctgaagacc ttggagagga
    15001 gatcccgctt gtccctgata cgacttttct tacgatcgat tacttgaaag aaaagtggca
    15061 tgaccatgga taatccagag atctccgtcg taccagttcc agagactgaa ataaagaaaa
    15121 tcacattgaa gaacgactac ttagaagtcg ttcttctcaa ttacggtgcc cgcctgcacc
    15181 aaatcttcgc accagacaaa gaaggaaaat cagaaaatgt tttattgtct tacgatcgtt
    15241 tcgaagatgt tctaacagac aaaagctttt ttggagcaac tgtcggacct gtagccggaa
    15301 ggatccggga tgcttcttgg ggtgagcata gattggagaa aaactgcgga tcacaccata
    15361 tccacggtgg cacgaaaggc tggtctttcc aattttggga agtagaagtt ttcaaaactc
    15421 catcatcaat aggtgtggtc ttttacttaa aagatgaatt ttctacctat ccaggaccaa
    15481 tcactgctac gatcacttac cgtttaacaa aaaacgaatt agagatgaca acctctgcta
    15541 gtagccttgt tgaaaccatc tgcaatccga ccaaccatgc ctacttcaac ctttcaggaa
    15601 atggaacaag agacatctat gaacatcagt taacggtctt tttagatggt gtgcttgaat
    15661 tagatcaaga aaaactgcct actggaaatt ggacaaaaaa agaaaaactg ccaatcgact
    15721 ttagaaaatc cccaacactg caagagatcc ttgcatgtta tccagatgga ctagatgatg
    15781 tatttctttt gcatcatcct aggctttcca aaacttcctt acaattattc gagaagcatt
    15841 ctggtcgcca aatgactata gcaacttcta ataaaagtat ggtattattt tctacgactg
    15901 gattcgaagc tgatttctct gtcaatggaa aacagatgca ttcaaactac ggattagcaa
    15961 tcgaaccgca agaaatccct gatatcgttc atttccccaa atttggatcg atcaacctcc
    16021 atcctggtca agaacggatc agtcagacga tttatcggtt ctctgcacaa taacaagaga
    16081 aaggtgtttt tatcatggct attaccgtaa aagatgtggc gaaaaaagcc ggtgttgcca
    16141 cctcgaccgt ctctcgtgtc atcaacgatc atcctagtat ttccgagaca acaaagaaga
    16201 aagtccgaaa aattatggat gaactaggtt atgttcctaa catgactgcc cgcaatctag
    16261 gaaaacgcat ttccagtgcc atcggcgtaa tcttaccccc tcttgattca aaagagcgga
    16321 tcggtaaccc attttatctt gagatcatgg aagcaatcaa cgaagaagcg cggaattatg
    16381 atatgactac agcaatcgct actgctaaaa attttgaatt gctgttagag aacgtccagc
    16441 gtatgcattt acaaaaacaa gtagatggtt ttattctagt gtattcagat agtcaagata
    16501 ctgtcattga ttatttatac caaaaccaca tccctttcac tttgatcgga cagccttatc
    16561 atcatgagaa cgaaatcgtt tatgtagata atgataacca gcttctaggt aaacaagcaa
    16621 cagaattttt gatccagaat ggacataaga atatcctgtt tgtgacaaac acgactcatg
    16681 aaagtttgta tttcgaacga tatttcggtt atcaaaaagc aatgatgatg gctggtcttc
    16741 ctgcgttatc cagtgtagta ctagagcatc cggaggatta tctgtccttt gagaaaacgt
    16801 tgaaagaaaa cgaagccaca gcattggtgg tcattgacga tatcttcgcc cttcgcacga
    16861 tgcagttggc gcaaatgtat ggctatgtca ttccagagac actctccgtc attagcttca
    16921 acaactcgat cttctcaaca ttaacacacc cttaccttac aagtatcgat atcgatatct
    16981 ccgaattagg acgtgtcgct atgcaaaagc tgcgcgaatt gatccatgaa gaattgagca
    17041 gtggcgtacg tttggtcatc cctcatcgct tgatcaaaag agagacagtc gttcctttag
    17101 aaaacccata ctaaatagct gactcactaa atacgctgac catttatcct atggtcagct
    17161 ttttatgtaa aagaagatac tacttctaac acattgttta tctgtaaaaa agagcgtggg
    17221 acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc ggaagcagaa
    17281 gcaactcctt cggaaataag ctgaaattca caaaaattta aaaagcaatt ttcttgagtt
    17341 tcttcttatt tcttggagtt aaacgcttct gtcccagcct cttttttcct tccttcaatt
    17401 ttttacacgt cgacgattca ataaaaaacc gcctaatatt gtaaagacag gtgcaagaat
    17461 cgagaaaaag gcgaatggca agtaatgcaa cgcgctaact ttcatcgttc ccataatgaa
    17521 tgtgccgctg actccccacg gtacgagaga attgacggtc gcacctgaat ccgctagtgt
    17581 tcgtgtcaaa taggtttttg ataagccaac gtcatcaaaa gaacgcttga atgtttctcc
    17641 tggaagaatg atggacagat actgttctcc aacgatcaga ttgattccga tacagctcaa
    17701 ggcagtaaac ccgactaaac gagaaggtcg gtccatcttt tcttttaatt cttgaacgat
    17761 ggtatcaacg atcagatatt tgatcagtaa cccacctaat gccaatgcaa gtaaaatcaa
    17821 actagcagaa cctaacatac tcatgatccc gcctcgagaa agcagttcat ctagctttgg
    17881 atcaggtgta tttgcgacat aaccattcat caagatgtct gctgcttttc cgaccgtcaa
    17941 acttttctca tggataaaag aaagaacgag agcagctgtc gatcccgcta gtaatgtagg
    18001 gatagccgga atttttttga ttgccccccc aaataaaata actagtggaa tcaatgtcca
    18061 aggagagatc caaaagctcg ctttcatgtt ttggatcatt tcattgatac tagctagatc
    18121 ggctgaatga ctgttatgtc ctaatatcgt ataaaaaatc aacgaaagga cgaatgctgg
    18181 gaaatccgta taacgcatac cagcgatatg ttcgaataga ttaactcctc caatacccgc
    18241 tgccaagttg gtcgtatctg ataaaggcga gcagttattc cctaaaaagg caccggaaac
    18301 gactgcgcct gtagtcattg catcattgaa tccaagtaaa tgaccgatcc ccaaaaaagc
    18361 aattccaatc gtcgaaatcg ttgtaaatga actgccgaca gtcactccga caattgcaca
    18421 aacaagaaat gctgtcggta aaaagaaacg cacagaaaca agtttaaatc cataaaccat
    18481 aatcgtaggg atggttccag ataaaatcca tgaagcaact aagacaccaa tcaaaagaaa
    18541 aatcaggatt ggaatgatcc ctggagtgat tcctttgatg atcccgtcat ggatctcatc
    18601 ccatgtgaac tttttaaatc tgccataaaa caacaaacac atgaaagctg caaggatcgg
    18661 gatatgtggc gagagctgga atccgatgat cagtactcct aggagtgcca acaacacgat
    18721 caatacagcg atactttctt ttaaagaaat atttttattc atcgtcgatc tcctatttca
    18781 aatgaaaagg ccctgccatg aaaaaactat tcacttggca ggacctccta ctaaatcagc
    18841 gacaaaacgt cggctgaaag acttattttt ttatttttct tctaaacttt tttctacctc
    18901 aatcccccaa gaatctttct tgaagtgatt gatgatcaat ggaataacga acatgaccac
    18961 tgtgatagct gctactaata atgcatcact caatgtcgct gtacctaatg tagcaaatac
    19021 gacaacgatg atagagaaga tcagcatgca gctgactacc caagctaatc cgtttccgtt
    19081 tttaccaatt cggtaaggac gttcgaattc tggatgtttc ttacgcattt taagcaatga
    19141 aacagcaatc aaaatgtaca cgatacaata aataacagtt gtagcatttg taagtgtcaa
    19201 gaatacagca cttacgtcat ccattacacc ataaagcaaa gcaaataatg aaataacgat
    19261 tgattgtgtt agaacgacat ttctagatac gccgtatttg ttttctttat ggaatccgaa
    19321 ttttggtgga aggaatcctt cacgagctac ttggatgatt gtttttgatg gtccagtaac
    19381 ccaagctgat aattgtaaaa gcacaccaat aaataccatg aagctgaaga tatttccgat
    19441 cacttctggt aagcctagga tttgacagtc aatcaagata ggctgcgtga tatttgctaa
    19501 ttccattttt ccatctggta cgacatttgc tacaagcatc gcattgatga cattcaataa
    19561 aactaatccg atcaaagcga taaatacgcc ttttgtataa ttttttgttg cgtctttcaa
    19621 acgaggcatg taaacagaac tcatttcgat cccaacaaaa ataaatgtga taccagctaa
    19681 gtatttcaat gaatcaatat gttccaagtc agggaaagct tttgaccatg aaaagtcacc
    19741 tagatagccg tttgagacta gtccgacttt gaaggctgcc aaaacaccta aaacgaacat
    19801 gatgactact gggatatata cccctagcca tacaccgatg ttcccgccga cttttgccat
    19861 atcgaatttc aagttcaaga tcgtgatgat ccagtagata actaaaatac aacctaaaac
    19921 gaaccagtga ttattgccaa gttctacatt accaaatgtg tttcctaaca atggtcctaa
    19981 agtagaagcg accattacca tacctgggaa catttgcacc cataatagcc atgcaacgac
    20041 aaatccccat ttgctgccca gtgctgtttt tacccataac tgaggaccgc cttcttgagg
    20101 aagcatcgtg gaaagctcac cggaaaccat tgatatcgga ccggcaaaaa acaaaactgc
    20161 aaaaattgaa taaaaaatca atgtccaacc gactgcggct aacgtaggaa tactccgaac
    20221 cgttgcacac agtgccattg tcagaccgat aaaggtccca agacccattt tacttacttc
    20281 agatttactc accgttgaca ctctccttga ttttttggat aaaataacgg tgtctttgaa
    20341 gtagtttcag caataaacga aacaattcag cgaatatttg aggggattct ttgagtttat
    20401 tgcttgaagg gtttcttcaa agacaacctc aaaacaaagg gatcgaagag cttaggtctc
    20461 cccttgctct tcagatccaa acatgcgtca gaaacagtta gtattcaagc tgttttctaa
    20521 catgattacc ttgcttattt tgcttcgctt gccaataatt ggtctgcgta gattttttcc
    20581 aatttttctt gaagagctgc tttgattttt tcagcatatt cttcaaagtg ttcttctttg
    20641 ttcatgtatg gtgtcattac gctggcacgc aatacagtta cttttccggc acggttccat
    20701 tcttcatctg agaatcctag ttggttcaca aattgtaatg ggctgttgcc atagtctggg
    20761 atagcaaagt cagtatgtga agttaagaac tcattaccgt aaatgcttcc tttcacgtat
    20821 gaagaataat catatacgtc atggttcaat ttattcattg caactaagtc atcgttgcct
    20881 ttttctttga atacatagtc taccatattg aaatctggat aagttaatgg atgaacttcg
    20941 atttctttgt ctcctacttt aaatgacaag tcattcaaga agttgtagaa acgatgtgca
    21001 ccttcgattg atgcacccat taatttacca tatcctgtga cattcaaagg taagacatgg
    21061 tgtgctgccc aaacgctggc tgctgttgcc cctgcttttg aaccttccaa gatataagcc
    21121 ccaagtaatg ctgggatatc tgcgcctttt tcaaatacat atgtcgcaaa gtaagagata
    21181 acgtctctca tgcggatatc tttgatgacg ataccaccgg cagaatatgg aacatagccc
    21241 attttatgtg ggtcaatcgt tacagattct gcttcttcga ttgctttata tgcactgtgt
    21301 acttcttcta agatataatc tttgttttct gtaaagacat tgtatttgta atgaacatct
    21361 tttaaatctt caaatggaat gaagttgttg tcttcgtcta agaaaatcgc acggccgtat
    21421 ccgccataag cagcatctac gtgtaagtag aagtaaatac cgtctttttc taatacgcga
    21481 cgcaatgcaa cgattttatc gataccgtcg attgctcctt cttctgttga tcctacaaca
    21541 ccgactacac ctaggattgg tgttttttca gctgctagac cacgaacgat tttttcaagt
    21601 tcgttgatgt ccatgcgata gttgtggtca actggcactg gaatcacttg gtctaaaccg
    21661 ataccgatga tgtcggcagc ttttagccat gaatagtgtt tagtttgtgg aaccaaccat
    21721 tttcctaatt tttctaaatg ttttccgcta cgtgctgagt gagctttgat ttcgtcgatt
    21781 ttttctggga cgctgtctaa cagattcatg atttcttctg ttgacaagtt cattaattcc
    21841 caatcgcttt ttccagcaac tagttcaggt gtcacttctt tcattgcaag cggcaatgat
    21901 ttgatgttgc gggcatacca aagtccttct aagtttgcta aagaaccatc agcaacgatg
    21961 tggccccagc catctttata gctcattaat tttgcaaatt ccattcctac ttcttcttcc
    22021 atttggcttg ttgcaggaga agattcatag gcaacgttat taccattcca tagcatagca
    22081 aaattgtacg ctagtaaaga tggcatcaat gtttcagagt tcatatgacc ccagtaacgt
    22141 ccggcattat gccaaggaac ggaatgggta cgcatccgcg ctgaaatttc tgatagaaca
    22201 tcctttgttt tattcacagt gtgttcaaaa ctagcactgc ttttttcttc tggcgtaata
    22261 ataggcatgt cttgaggcat gtagttttga cgccagccta aatgttcatc caccaattca
    22321 ttcaacaaag ctttatagat ttggccgttc tcagctttgt ccccaatgaa tagggcattt
    22381 aggtttagat ctttcgacaa tgattcactc atagtgaaac cctcctaaaa aaatatttaa
    22441 attttgtcga gccctacatt acacctgttc ttctattctg taaactaagt tttggcctga
    22501 tttttaatca tattttaata tgtatcaatt atccgaattg ttcacacttt tgaaataatt
    22561 aaaaaaaacg agggaattta caaatgtaaa ctccctcgca aagattgtat taacgacaac
    22621 cagcgttcta ttttaggatg ggtttcaaaa ttggaatgtt gtaatagtat ttcccttttt
    22681 caaggtaaaa acttactaaa ttctaatcaa ttactttggc caaaaagtaa tttttcttac
    22741 cttttcgaat aaccacaaac ttaccatcga attcatcgga tggatttatg acaaaattta
    22801 aatcggtaac gcggtcaccg ttaatactta tagctccgtt tgaaacatct tcacgtgctt
    22861 gacgtttaga tggttcaatt tttgtggaaa caagcagttc cacgatattt tcaggtgtgc
    22921 ttgaaatttc aacgttcggc attttaccga atccttgcgc gatttcttcc gcatttagtt
    22981 ctttaatatt tccagagaac aatgcttgcg ttattttttg cgcttctttc aagtcttctt
    23041 cactatgcac aaatcttgta acttcttctg ccaaacggcg ttgtgcttca cgtttctcag
    23101 gttctgtctc tacttttttc gctaagtctt cgatttcttc ttgagacaag aaagtaaaga
    23161 atttcaagta tttcactaca tcgcgatcat cttgattcaa ccagaattgg tagaactcaa
    23221 atggtgaagt tttcttcgga tcaagccaaa cggcaccgcc tgcagttttt ccgaatttcg
    23281 ttccgtcagc cttcagcatc aatgggatcg ttaaaccgaa cgcttctgct tctggtcctt
    23341 ctaattttcg gattaggtct aatccagctg tgatatttcc ccattggtct gctccaccga
    23401 tttgcaattg cacatcgtat gttttatgca gatgcaaaaa gtcgatagat tgcaggattt
    23461 ggtaagtaaa ctctgtaaat gagattccta cttccaaacg gctggcaacg atatcttttg
    23521 ctaacatcgt gttgatgttg aagtttttac cgtagtctct caaaaattca agtaaagaga
    23581 tttttgacaa ccagtcgtag ttgttcacaa atgtgatatt tgcatcttta ccaaacaatt
    23641 ttctcatttg attggaaagt gcgtccacgt tatgttgcac ttgttccatc gtttgcaaca
    23701 cacgttctgt tttgcggccg cttgggtcgc cgatcgttcc tgttccgccg ccaatcagaa
    23761 tatatggatg atggcctgcc aattggaatc ttttcatcat cataaatgga atcaaatgtc
    23821 cgatatgcat cgaatcacca gttggatcaa cgccgcaata aagtgaaatg ctcttttctt
    23881 caacgagctc gcgaagtctt tcttcgtttg tttgctgatt aatggcgtcg cgccaagtta
    23941 attcatcaat aatattcatt ttactgacct ccgtgtagtt tttttatgaa agcaggaacc
    24001 tgctagcata tgtaattaat tatttcttca ggataggata ggaaataaaa aaagtccctt
    24061 ggcatataag ccaagggacg aaatctcgcg gtaccaccca atttgtatgt ctagatatcc
    24121 aacatacctc tcgaacattt aacgctgtcg cgtggcaatc actgccaaag actcctgaaa
    24181 tgtaattcgc tttaaagcat gtactggttt gcagcgacca ccagctttct gaaacattgg
    24241 ctttaacaac tacttcgttt cgatcttcgt cttgttatta ttaatatgtg tctatcatag
    24301 agcgcaactt tttctttgtc aacagaaaaa ggatattttt tttaaattcc tcattttttc
    24361 ttttttgaat ccgctctaat tccgaacatt ttattttcaa ttattgttaa aagaaataac
    24421 tttttctgta ttttttctgc aatttatcga gaagtcactt tactttgcct cttattcttt
    24481 cttcgttaca ataaggaatg ggacaagtaa aaaaatgaaa aagcaaggga cgaggtatat
    24541 ggaaaaaaag caaaaaatta ttttaggagc cacttctgct ttaactatcg ttattttaat
    24601 tctaagtttg ttttttgtca tccgatcatc tacaaaaaaa gaaccaatca ttcctgaaaa
    24661 aaaaataaca gaaaaagcaa aagaaccgac agatgataag caacaactag ttactcaagc
    24721 aaaaaatctg gctcaaagct acgattatga ccaagcgatt gcgttattgg aaaaagatag
    24781 taagaacgga tctactgaaa cacaacagct tcttcaaaca ctaaaaaagg agaaagaaca
    24841 actgatcgac tggtctgatc ctactcagat ctctcatgtc tttttccaca gcttgatcgt
    24901 ggacccgcaa aaagccttcc actctcaaca ggcacaagga tacaaagatt atatggtgac
    24961 tgttgaagaa ttcaaccgtt cgatcgaaca actttatcaa aatgattttg ttttagttaa
    25021 tttgaatgat ttgatccaaa aagacgaaca aggaaatctt tcctttaacg ggcttgcgct
    25081 acctgaagga aaaaaaccat tggttctttc acaagatgat gtcagctatt atgaatatat
    25141 ggataattcc ggttttccta gccgattggt cgtatcaaat aacaatgaaa tcaaaaatgt
    25201 ctataaagag aaagataaag aagaaatcgg tgactttgac atggttcctc tgatcgatgc
    25261 ctttgtcaag aaacatcctg atttttctta tcgaggcgca aaaggtacct tggccctgac
    25321 aggttacaac ggagttttag gttatcgaac atcaaaatct caatatggag acaatgaaaa
    25381 aacaaaaaaa gagattaaag aagctaaaaa agtggcggaa cagttgaaga aagacggctg
    25441 gagctttgct tcccatacat ggggtcactt gaatatgact caagcttcac tagaagatgt
    25501 aaaaaaagac aatgaattgt ggcagcaaga agttgcacct atcctaggaa aaacaaatat
    25561 tctgatttat ccatttggtg cagacatcag cgactggcag ccttattcag cagaaaatca
    25621 aaaatttgct tacttgaaga gtcaaggttt cgatattttc tgtaatgtcg atgcttctac
    25681 accagcttgg ggacaattag gtacggattt ctatcgaaat gctcggatca atatcgatgg
    25741 cattcgtttt caatctgatt tagacggaaa aaatcctatt cttgatccat tcatcaatgt
    25801 ccatgatgtc tatgacaaag aagcacgcga atctgcttga ttttacgtca tttatgaacc
    25861 aaaaaagccg aactgacatt ttatcgtcag tttcggcttt tttatgcttt atttcttctt
    25921 cggtgcgatt cccttgatca ggacttctgt ccattctgct gtatatggaa gcagttttgc
    25981 gtatatctcc ggcttagaaa ttccttctac cattgcttga aaatagagga tcaagaactc
    26041 atccgaatat ttcaaatcca cttttccttc tttccgccct cgatggaaaa gatcaagcat
    26101 gatactgaaa ctttcttgcg tatacgcatc catcgcgtca gaaaattctt ttttttcttt
    26161 ttttgtatag tatgtcatca aatcgatata aaattgtttg ttcactcgat tgagataacg
    26221 gattttgttt tcactcatgg caatcagtgt ttcttcaaat ggaagatttt ccgcaatcaa
    26281 cgctttcgct tcttgcccta aatcattcat aaatgcaaaa aatacttcat aaatcagccc
    26341 ttccttgctg ccaaaatatt taaaaatcgt cgctttactt acctgagcgt tctcagctac
    26401 cttatccatt gtcactccag cggttcctat gtctgtgttc atcaagtcaa aagctgcttg
    26461 taaaatctct ttctttttct cttgtgctct cttttcaaaa ccgttcattt gtagagcctc
    26521 ccgtaatttt aaacttttat aatatatata gttcaaattt a
//
LOCUS       DADVXU010000031        26059 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.033, whole genome shotgun sequence.
ACCESSION   DADVXU010000031 DADVXU010000000
VERSION     DADVXU010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 26059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.033"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(225..677)
                     /locus_tag="KB953_001205"
     CDS             complement(225..677)
                     /locus_tag="KB953_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBB6866193.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            complement(690..1100)
                     /locus_tag="KB953_001206"
     CDS             complement(690..1100)
                     /locus_tag="KB953_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6866194.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            1375..1587
                     /locus_tag="KB953_001207"
     CDS             1375..1587
                     /locus_tag="KB953_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6866195.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            1590..1802
                     /locus_tag="KB953_001208"
     CDS             1590..1802
                     /locus_tag="KB953_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866196.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            1942..2097
                     /locus_tag="KB953_001209"
     CDS             1942..2097
                     /locus_tag="KB953_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866197.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(2094..2318)
                     /locus_tag="KB953_001210"
     CDS             complement(2094..2318)
                     /locus_tag="KB953_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HBB6866198.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            2396..2584
                     /locus_tag="KB953_001211"
     CDS             2396..2584
                     /locus_tag="KB953_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866199.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            2910..3245
                     /locus_tag="KB953_001212"
     CDS             2910..3245
                     /locus_tag="KB953_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866200.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            3478..4419
                     /locus_tag="KB953_001213"
     CDS             3478..4419
                     /locus_tag="KB953_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HBB6866201.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            4421..5311
                     /locus_tag="KB953_001214"
     CDS             4421..5311
                     /locus_tag="KB953_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HBB6866202.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            5354..6154
                     /locus_tag="KB953_001215"
     CDS             5354..6154
                     /locus_tag="KB953_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBB6866203.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            6169..7020
                     /locus_tag="KB953_001216"
     CDS             6169..7020
                     /locus_tag="KB953_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBB6866204.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            7059..7220
                     /locus_tag="KB953_001217"
     CDS             7059..7220
                     /locus_tag="KB953_001217"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866205.1"
                     /translation="MLQIIGDRDMDELSQQESRMVRKISKLIIYRDTERFLTVLGNVY
                     ENPELLEVE"
     gene            7221..7682
                     /locus_tag="KB953_001218"
     CDS             7221..7682
                     /locus_tag="KB953_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBB6866206.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            7708..8160
                     /locus_tag="KB953_001219"
     CDS             7708..8160
                     /locus_tag="KB953_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866207.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            8266..8460
                     /locus_tag="KB953_001220"
     CDS             8266..8460
                     /locus_tag="KB953_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866208.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNGFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            8491..8877
                     /locus_tag="KB953_001221"
     CDS             8491..8877
                     /locus_tag="KB953_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866209.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQAKMTQISHLRLEL
                     EDLRQIEKSIQLNDNQKIVLEWLKSETILTREAPILSVNAFSDKNLLGKLPDKVRKAY
                     KLLACKQEYEVLSAFAQWGLEQEEAE"
     gene            8874..9077
                     /locus_tag="KB953_001222"
     CDS             8874..9077
                     /locus_tag="KB953_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HBB6866210.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASNAIRKFGISILNDDRKWEEMR"
     gene            9074..9370
                     /locus_tag="KB953_001223"
     CDS             9074..9370
                     /locus_tag="KB953_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866211.1"
                     /translation="MKIKDGFYASSHGIGGLMLDMPIKNPKTRQKSKFKIGDMVRCKA
                     EEFIYPFRGYVEHVYNHSAIIHIENTMECDKWLAKSKDNLAVARLVDMELINDK"
     gene            9446..9862
                     /locus_tag="KB953_001224"
     CDS             9446..9862
                     /locus_tag="KB953_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HBB6866212.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
     gene            10123..10260
                     /locus_tag="KB953_001225"
     CDS             10123..10260
                     /locus_tag="KB953_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866213.1"
                     /translation="MDKEIKVTVKLDLTELKELLNKASDQVEQLQETLDEIANFKIQV
                     S"
     gene            10670..10900
                     /locus_tag="KB953_001226"
     CDS             10670..10900
                     /locus_tag="KB953_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866214.1"
                     /translation="MRNYWYISLTNEYPRTIDDCSVRVVRSVQIKKKYSIVEMTREAT
                     PKEIDKCKLLYCGHGFYDEPNIQNNINKNLRD"
     gene            11059..11892
                     /locus_tag="KB953_001227"
     CDS             11059..11892
                     /locus_tag="KB953_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small subunit of terminase"
                     /protein_id="HBB6866215.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELKSNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPKRNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            11885..13300
                     /locus_tag="KB953_001228"
     CDS             11885..13300
                     /locus_tag="KB953_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HBB6866216.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPENMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTTVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            13312..14841
                     /locus_tag="KB953_001229"
     CDS             13312..14841
                     /locus_tag="KB953_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBB6866217.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEYMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKDPTAKAEYVTKKLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            14759..15811
                     /locus_tag="KB953_001230"
     CDS             14759..15811
                     /locus_tag="KB953_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBB6866218.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            15813..16130
                     /locus_tag="KB953_001231"
     CDS             15813..16130
                     /locus_tag="KB953_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866219.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            16257..16880
                     /locus_tag="KB953_001232"
     CDS             16257..16880
                     /locus_tag="KB953_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HBB6866220.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            16895..17785
                     /locus_tag="KB953_001233"
     CDS             16895..17785
                     /locus_tag="KB953_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HBB6866221.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            17808..18023
                     /locus_tag="KB953_001234"
     CDS             17808..18023
                     /locus_tag="KB953_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866222.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            18035..18376
                     /locus_tag="KB953_001235"
     CDS             18035..18376
                     /locus_tag="KB953_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866223.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            18376..18747
                     /locus_tag="KB953_001236"
     CDS             18376..18747
                     /locus_tag="KB953_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866224.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            18740..19135
                     /locus_tag="KB953_001237"
     CDS             18740..19135
                     /locus_tag="KB953_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBB6866225.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            19137..19514
                     /locus_tag="KB953_001238"
     CDS             19137..19514
                     /locus_tag="KB953_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866226.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            19515..20123
                     /locus_tag="KB953_001239"
     CDS             19515..20123
                     /locus_tag="KB953_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBB6866227.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            20123..20464
                     /locus_tag="KB953_001240"
     CDS             20123..20464
                     /locus_tag="KB953_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866228.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGPVVEEAAEVMEG
                     MQNAVDGKSVSEALSRAYSFIADILFEGQFTGEDYRQGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            20706..23495
                     /locus_tag="KB953_001241"
     CDS             20706..23495
                     /locus_tag="KB953_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBB6866229.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANSFVLIIGAVVGAI
                     AVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNGIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWLNIKYFFINLWIDIKYFAIQKWIELKFSIIQTWID
                     LKYNAITTWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVN
                     SAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTI
                     KGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTP
                     SVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQ
                     MKRDAGAVGGTLYAT"
     gene            23485..24225
                     /locus_tag="KB953_001242"
     CDS             23485..24225
                     /locus_tag="KB953_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBB6866230.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            24222..>26059
                     /locus_tag="KB953_001243"
     CDS             24222..>26059
                     /locus_tag="KB953_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBB6866231.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCEIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
BASE COUNT         9468 a         3708 c         5319 g         7564 t
ORIGIN      
        1 tctctatcat gtaatttatt tagtttttca tcattttttt gttctaattc ataagcttct
       61 ttttttattg tgttagcttt cgacagttta gtgattccta taattaataa aataagtcca
      121 atcaataata acattcaaaa ctcctcattt ctttgatata attattgtag agtttactca
      181 gaaatgagga aagagtccat gtgaaagcat gggctttttt atttttaatg ttgtaatact
      241 aaatgttcaa gattatgagg aagccctaaa tattcaataa tttggaactg actcatatca
      301 gataaatctt gttcatctat tgttctagtt aacatttcaa ttgcaaacat attagcttca
      361 gcttctatac cataaatcat acaactaagg ttgtttgctc taaaagctgc tgtgcaaata
      421 cctttatgga gtctaacgtg ggctatttca tgccataata caaatctttt catagaaggt
      481 gataggtttt gattaattaa tatcacataa catcttttat tgtgtatcgt ttgtcctaat
      541 gttttattcc ctaaatcagt ttcaattaaa tctatatcag aatatttgat tattttatat
      601 ggattaatcg catcagatcc aaactctaca aataattttt ctatttcttc taatatccag
      661 tatttgttta aatgcatacc aaagcccacc tattgacgat attttttagg agtgaatttt
      721 ttctttgctt taactttggt taaggcaagt gcaagttgaa tggcatcgta taatttttcc
      781 ttatcttcat cgtctaaagg ttcaccataa taatttgttt cagcatctga attcaagcct
      841 tcaagtaatt tttcagcttg taaagcaata tcttgtttat ctttttctgt taaatcataa
      901 taatggcgtt tatcagtacg tcctaaaaga taatcagttg atacattaaa gtaatctgca
      961 actttttgta ttttatctcc agatggagta cttttattcc atctagacat gcttccattt
     1021 ccgaaattta actttctttc aagttctgcc aacgtcattg aacgttcttt taataagatt
     1081 tttatacggt ctaatagcat aggatatgca ttcctttcaa aataccgaca aaaaaagtat
     1141 ttttctgcaa attatctatt tacaaataga gaatttgcag ttatactagt tttgtaagca
     1201 agatatttac ggcatacaaa gactaaaagc aataaactaa tagtctcccc agacatcagt
     1261 aagctcgctt tttgtgcgct gtattttgta tgcttacata atagcatatt tgcagttttt
     1321 gtcaaactta ttagacaaaa atattagaaa atattcgata agggggtatg aagcatgact
     1381 ttaaaacagc aagtattaat tgctttagta aagaaaggtt ggagtcaaag agagttagct
     1441 cgacgaatgg gtatatcaat tacttattta cgagatatct tcatcgaaaa aagaaaacct
     1501 aaggaacgtt taaaacaaat cgaagaactt ttggatatca agttagaagt agattctaat
     1561 gatcagccat cagaacagga ggcgtaaaaa tggtacaacg tttgagcaaa gcggagttta
     1621 aagagaaata taagtattct gaaagtactt atcaaagaag aatgaaggaa tttaaaaaat
     1681 cacgttttag tgaggggtat aacgcagtta cttccaaaga aataacaatt gatgtcgatt
     1741 tatatgaaca gtttagaaca tggcgttcta aaaatcgatt tagagttaga gctgtttgtt
     1801 aatagctaaa aaatctaaat tcaccacttc tgaaaagagg agttgctgct ctcctttcaa
     1861 gtttgccaaa gtccacgttt tttcattttg gcaactcctc ttcttggagg tggtgacggc
     1921 gagagacgaa ggagggatga catgaaggaa gaatcaaaaa tagggatttt gaatgagaac
     1981 aattacacaa gattaattat tgaaacagaa gaagggaaaa aagtagcaga agttacattg
     2041 accgatgcaa ctcctgctga cggttatcgt attcgattaa ctcctaaata tgattaacct
     2101 tttggtgggt gtggatcgtt tccgtgacta tcacggcttt gaatttttcc atctctgcca
     2161 tgaataaata gctcagatcc ttgattttta gaaatctctc tagcgatttt tactgcttct
     2221 gattttgtat tagtatgtgc agtcgcttta gaatttccag cacctttgac attccaacca
     2281 ccattggaat taggtacaac atgttgattt tttcccatag ttttcacctc catttctata
     2341 gaaatatttt atcaaataga aatggagaaa ttcaataaac gaaaggagaa gtgatatggc
     2401 atataccttg caacaagaac atcaaattct cggtttgatt aaacaacgca ggaaacaatt
     2461 acaagatgac cgtgcagcgc ttagaaaagc cgatgagcta tcagataggc aagctgaact
     2521 aattgcttct gaacttgagg atttgagaat gctagaaata aaaaataggg agattagatt
     2581 atgaagaaga cagacacact ttttatagga ttcattttgg ggttattagt gattgtagcg
     2641 caccaaagta ttatcggggg aagcttgttc gcagcattga tggttttaat caatctgctt
     2701 gattcaaaag aaaggagcaa ctatggcacg agaagaagcg ctaaaaatcg gtaaagtgat
     2761 tgctgataat cggtggggaa atagccgccc tattatttta agcaagcaac atatcgaaaa
     2821 gcaaaaagca tggcaacaaa taaaaaagtg actctgccag ccagcaaaga gtcacataca
     2881 aatttataaa actaggagaa ttatatcaca tgaataagaa aattgaaaat cttatcgaag
     2941 aattgaaacg tgaatgccaa aagcaaggtg tttctattat ttgtacagcc caaaaagaag
     3001 gggaacttaa aagtctcgtt tatggtgaga caacagaaat tttactttgt cttgctatgc
     3061 aagaagaaca tttagatgaa aatctcccat tgtcagcaca tattatgcgt agaattgctg
     3121 tggatgctta cgaacaagcg aaaaatgaag aagaaaatca accttcaaat catacttttg
     3181 ttattaataa caaggaagat ttagctgatg tgatgactcg tattctcaag ggggaatttc
     3241 aatgatgaat gaaaaaacaa ttgatctact cattatttta tttttcacgt tagtgattgt
     3301 agcgaaaagt tatgtaatct ttggcttgtt attcgcaatt accatgcttg tgctttgcgt
     3361 gttagcaagc agaaaagagg ttaaacatga acgagttagc aaatcttgac aactatttaa
     3421 ctgatcctga ttatgctaaa ccaccatatg aagcaccaat tgatgaggag gatgaagatg
     3481 agtaaatcta ccttagaaat gagccatcaa gaatggcttg aagaccgtaa gaaaggcatc
     3541 ggaggttcgg atgttggaac aattttagga ttgaataaat ggaaatctcc ttatcaacta
     3601 tggctagaaa aaacaggaca agtagtactt gaagaatcag gaagtgagcc cgcttattgg
     3661 ggtaatgttt tagaagaagt ggttgctaaa gagtttcagg aacgtacagg caaaaaagta
     3721 cgcagaagaa accaagtatt tgaacatcca ttacatccat ttttaagagc aaatattgat
     3781 cgggaagtag tgggagaaaa tgccattctg gaatgcaaaa cagctaatca atttctcggt
     3841 aaagaatggg aaggcgaaga ggtaccgctt agctatctct gtcaagttca acattacatg
     3901 aacgttctaa acaaagacta ttgttacatc gctgtcttaa tcggtggtca aaaatttatc
     3961 tggaagcgga ttgaacgaga tcaagagctg atcgatacaa tcactgaaca attagtagag
     4021 ttttgggaaa caaacgttct tggaggtatc gagccaatta ttgatggtag tcaagcgaca
     4081 gctgatttct tgaaagagaa gtatgcagat gttgaagacg ttcaaacgac gttaccaatt
     4141 catttcgatg aactagttga acagaaaaac gaactcaaac ggactaagaa agaaattgaa
     4201 tcagctatcc gtcaagtaga caacgagatt atcagcgagc taggtaaacg tgaagccagt
     4261 atcggtatca ctcaaaaaaa catcatcagt tggaaatttg tccgtacgag acgtatgaac
     4321 tcgaagaaac tagcagagaa atatccagat gtcgcaaatg atgaagagat ttataacgtt
     4381 actgaatcaa gaaggctaac cgaaagggag atcaaataat atggcaacaa atgaatcgtt
     4441 aaaaaatcaa ttggcagcaa agccacagaa acaagttgca ccaggtcaat taggacttaa
     4501 agctttgatg aatacaccaa caatgagaaa gaagttcgaa gaggtgcttc atgacaatgc
     4561 taatgctttt atgtcgaatg ttatgactct tgtatctaat gacagttatc ttgcagatag
     4621 tgaaccgatg tctatcatga gtggtgcgtt aactgctgca acattaaatc ttgggctaga
     4681 taagaattta ggttatgcat atttagttcc attcaatagt aaaaacaagc aaacaggaaa
     4741 atgggaaaag aaagctcaat tcatgcttgg ctataaagga tatatccaat tagcccaacg
     4801 atcaggtaaa tacaaagcat taaatgtgat cgaagtttac gaaggagaac taaaaagctg
     4861 gaaccgactg acagaagagt ttgagtttga tccaaatggt agaacgtctg atgaagtcat
     4921 tggatatgtt ggctatttcg agttactgaa tggattcaaa aaaactgtct attggaccaa
     4981 acaagaaatc gaagctcatc ggattgctaa caataaagat cgcgataaga caaagttaag
     5041 tggtgtgtgg gcatctgatt acaatgcaat ggcacgaaaa actgttttga gaaatcttct
     5101 ttctaaatgg ggaatcttgt ccatcgaaat gcaagaagcc accacatcgg atgagagagt
     5161 ccaaagggtt caagaagacg gcagcattat tgctgaaaca gaagttgaag aagatattcc
     5221 tgaaagaaaa gaagcagagg ttatatctga agaaaatgaa gatgtacaaa ctggattatt
     5281 tgatgcatct aatccgccgt taaacaaata atgagggagc tttctccctt gcttttctag
     5341 aggagaaata cgaatgaaca caggatatgt caaattacat cgaaaagtga tgaattcatt
     5401 cgtttggacc aatccttata tgtataaatt atggaacttg tgtttgatga aagcgagtca
     5461 tgagaatcgc aagatacttt ttaatggaaa agagatacaa gtgaacagcg gagaattcgt
     5521 cacagggcgc gatgctatca catctgagat gaacgaaggc gtgaaacgtg aacatcaagt
     5581 gaacagcggt tctgtatgga gatggctaaa acaatttgaa aaaaacggaa tgttgaacat
     5641 caaatcaact acgaaataca gcgttatatc aataaaaaac tggtctttgt accaaagcag
     5701 tgaacaacaa atgaacatca aacgcacaac aagtgaacaa caagtgcaca caaacaagaa
     5761 tgaaaagaat tataagaatg aaaagaataa taaaacatca tcgtcgcaac cacgcaaaaa
     5821 gcgtgtttac gacaccgact cagtttacta cattctcgcg gaggagttat tcaaacagat
     5881 ttgtcagaat caggaaatca aaaagccgaa tctgcaaagt tgggcagata acattcggaa
     5941 aatgatcgag atcgacaagc ggaccgaaaa ccaggtacgg ggaatgattg aatggagtca
     6001 gcacaatgtg ttttgggcat cgaatatttt atctgctaaa aaattgcgag aaaaatacga
     6061 cacaatggca gcgcaagcca atcgtgatta taaaacaaaa caaactaaaa cgcttgaata
     6121 cgagaaattt agcacagatg agttgcctat ttgagaggag gcgtaggcat ggaaactgtt
     6181 ggggaaatca tggaaaagct gatacagaaa gtgcttgtcc aacgtggcga atgtcctgaa
     6241 tgcggacagc ctttgtatgg atggcgtacg aagaaccctg atggctcaga acgttgtaag
     6301 ccaacctgta tgcaatgtgg gtataaagca ttgcgagttc aggaagactt acaaacagaa
     6361 cgaatttaca acgagagcct gaaagcaaga gcaatcaatt ttttcaaagg tggttctgtt
     6421 gtgcctaatc aagcgttgtt tgattgcaca ttgcagaatt atcaaattgt cgatcaagaa
     6481 acaagacaag cggttgaagt aaccaaacgc tttgttaatt cagtcttgtt aggaaatcca
     6541 agtcaccttg ttttaactgg aaaacaagga acaggtaaaa gtcacctagc gatggcagcg
     6601 gcttgggaag tcttgaagcg atcaaactac gataaaaaaa tcttatttat cgggttacag
     6661 gaaatgctgg atcaaatcaa attttcgtac aacaatcctg aactcagaaa aacgattgag
     6721 ggatcgttga ttgcagatat caaaacagcg gatttagtca tcattgatga tatcggttca
     6781 gaactaggaa aagatgcatc agatagtcga gcgtttggca taaacacgct aaattcgttc
     6841 ttggatgcac gacagaacct agcaacgatt atcacaacga acttgcttgg tgaagaactg
     6901 aaaaaagctt acggtacgag aacaatatca agaatgtttg tcaactctga tggatttacg
     6961 atggtatttt ctcaaacagc agacaagcgc ataaaaccag tcaaaggagc gatcgcatga
     7021 atttgactaa acaagaaaaa agaatctgtc gtcagataat gttgcaaatt ataggtgatc
     7081 gtgacatgga cgaattatct caacaagaga gtcgaatggt tcggaagata agtaagctaa
     7141 ttatttatcg ggatacagaa agatttctca ctgttttagg taacgtctac gagaatccag
     7201 agttattgga ggtagagtaa ttgccaaaaa tattagatgc atgttgcggt agtagattgt
     7261 tctggtttga taagaacaat tctgacgtaa catttatgga ttgtcgtcag cagtatgaag
     7321 aattatcaac aggtcatgtg atcaatgttg atccagatgt ggtcgcagac tttcgcgata
     7381 tgccttttga agatgatgtg ttcgatatgg ttgtatttga cccgccacac ttaagacatg
     7441 tcggcgaaaa ctcatggctt gccaaaaaat atggaaaatt agacgaactt tggccagaag
     7501 acatcaggca aggttttgca gagtgtatgc gagttttacg accttcagga acattaattt
     7561 ttaagtggaa cgaggaacag ataccacttt ctgatgtgtt agaggctatt ggagaacagc
     7621 cattgtttgg taataaacga agcaaaacac attggcttgt gtttatgaaa aaattcagct
     7681 aacggaggaa acaaaggagg aaaagtaatg aagattgtat taaataaatg ttttggtggc
     7741 tttgggttga gccatgaagc aaaaatggaa atttttaaaa gaaaaaatat tgaagtattc
     7801 ccatacatca ataactttag ctatgactca gatgatgaat acacgagata cacaggccaa
     7861 aagctaggtt cgatggattt tatctactat ttcaaaaaag acccgaaaat cgacaaagtt
     7921 acaggaattt atagcgaaat tgaccgactg tacggaattg ctgatgattc cagtttttca
     7981 agtgattcaa acagaggtga taaggacctc gttgctgttg tcgaaaaact aggaagtgag
     8041 gccagtggac catatgcaag tcttaaagtc gttgatatac ccgatggggc tgagtgggaa
     8101 ataagtgatt atgacggcgt tgaaactgca cattatggtt ttcaaacagg tagtatttag
     8161 tccactaatg acaactaaag aggaggcaga aggatgagtg aagatgaata ctttgaacag
     8221 tagtctatta acgacagaga tttttaactg gaaaggggtg tagtcatgga cgcatcggaa
     8281 gtagtaaata aaattaatag tctcaaagag gttttcggag acgtcgaagt agtgataaac
     8341 gcacaaggct atgacaacgg attttttaag cagattaccg acattagcat tcagcaagga
     8401 atggaagacg aagatggaaa ctttatcgat gaagtagcca ttttagtgac ttgcgagtaa
     8461 gtaattgaca gctaaagagg aggcagaagg atgaaaaaat tgcaccaact catatctgag
     8521 aaagaaagcg aactccaaaa tttagaagat agtttgggat tggggtttcc aattgttgag
     8581 caagctaaaa tgactcaaat cagtcatcta cgacttgaat tggaagattt aagacaaata
     8641 gaaaaatcaa ttcaactcaa cgacaaccag aaaatcgtgt tggagtggtt gaaaagcgag
     8701 acaattttaa ctagagaagc accaatatta tctgttaatg ctttttctga taaaaattta
     8761 ctaggaaaat tacctgataa agtacgcaaa gcttataaac tattggcttg taaacaagaa
     8821 tatgaagtcc tatcagcatt cgctcaatgg ggattagaac aggaggaagc ggaatgaaca
     8881 acagacatcg cagaataaca aaactaagaa aacaggaact gaatgtacta aagacaaagt
     8941 ttgaaaaaga atatggaatt tcagcagaag aaacatataa agtggcaagt cagtttgttg
     9001 ctgatgcgag taatgctatt cgtaagtttg ggatttcgat attaaatgat gatcgtaaat
     9061 gggaggaaat gagatgaaaa taaaagacgg attttacgct agtagtcatg gtatcggcgg
     9121 tttaatgcta gacatgccga taaagaaccc taaaacacgt cagaaatcaa aattcaaaat
     9181 cggtgacatg gtccgatgta aagcagaaga gttcatctat ccgtttcgtg gatatgtaga
     9241 gcatgtctat aatcattcag caatcattca cattgaaaac acgatggaat gcgataagtg
     9301 gttagcgaaa agcaaagata atttagcagt ggctcgtttg gtggatatgg aactaatcaa
     9361 tgacaaataa aaaagccgga tcgctccgac tgattcaata aatccaacac atttattata
     9421 tcacataaag gagcggtttg acttgatgca attgttacga gaggtagatt tcaaacagac
     9481 aagatgtaat gcgagagatg tgctgaagaa ctttcggcgt ttggagcgga tggcaggtcg
     9541 ctctttgata gatattaagt cgccgattat tacggatatg ccgaaggcac cgaagcacgg
     9601 caataaggca gaagacgcga tcattcagat gatggatata gaagcggaga gagacgcaat
     9661 actagcggct ttgatggctc ttagtctgat tagccgtcag atactctact acagcttctg
     9721 tgacgtaaac aagcactcta attatgaaat agggcaattg atacgaggat acggagagaa
     9781 gaatgtagag aagttgaaat ccatcgcatt aatcgaattt gcagaagcat acaaaaaagg
     9841 cgtgttagtt cagtatcgtt gattttgtag ggtttttgta gggatagtgt agggtttttg
     9901 agcggtttaa cgtgatatta tggtagtgtc gaaagattag tgataggtct gagacaaaat
     9961 aaaatgtaag ggaggaaatc tccctcatcg ttttaactta agcttcgata gacagcaacg
    10021 gaaatattaa gaataaggat gtgaatttca actccttcta aattgttctt attatctatc
    10081 atccgttgtt gtctattaat ttatgcatcg gaggaaaaac gaatggataa agaaatcaaa
    10141 gtaactgtca aattagattt gactgaacta aaagaactgc tcaacaaggc tagtgaccaa
    10201 gtcgaacagt tacaagaaac tttagatgaa attgctaatt ttaaaatcca agtttcttag
    10261 cagtatattt tttagcagct gcactcataa aatcatacca agtatcagaa aaatataact
    10321 taactaaata attaaaaaag gtgggtaaat agaaatgacg gcgttaataa tttcaatttt
    10381 tgcgctctgt cttaatgtct atatgattgg atttaaaaat ggacaaaata aaaaatagta
    10441 gcagccgaga ataattttat agtgtcactg tggcggaaat agaagacgca aaggtggagg
    10501 taggaagcgt atggcgcact atcgggacct tgtacaaaac tagaaaggac tagtacgtgt
    10561 gtggtgcgag ccctagggag gaacaggata accgcctgtg tgtaggttgc tattacatat
    10621 tagatcactc tttgagtggt ctttttattt acataaagga ggctacataa tgagaaacta
    10681 ctggtatata tcgctaacta atgaatatcc tcgaaccatt gatgattgtt cagtgcgtgt
    10741 tgtgcgttct gtccaaatca aaaagaagta ctccattgtt gaaatgacca gagaagctac
    10801 gccaaaagag attgataaat gcaaacttct ttattgcggt catggtttct atgatgaacc
    10861 aaacattcaa aataatatta acaagaattt gagggattag aatgcaagaa atggcttaaa
    10921 aatctccaat caggacaaat caagttagac aaagtttccg atttaaagat attgattgaa
    10981 gctgatttaa tgctaaaaga tattgaaaat tagaaaacaa aactcaacct aagaagattg
    11041 cgaggtggtg tgtattgaat ggcaagacaa cgtgatccaa gacgtgatga agccaaaaga
    11101 atttggttag aatccaacgg agaaaagcag ttaaaggaaa ttgcatctga attaaatgtt
    11161 tcagattctc aggttagaaa atggaaatcg caagacaaat ggagcgctga attgaaaagt
    11221 aacgttacca atggcaaaag taacgttact aatcaaggtg gcgctcctat tggtaatcaa
    11281 aatgctaaag gtaacaaagg aaacagcaga gcatcaccgc caaagagaaa taagaatgct
    11341 cttaaaactg gtgagtatga aacaatattt tttgacacgt taaccgataa tgagaaggac
    11401 atctattcta gtttgaatga tgatccttct tttgctttgt ccgaagaaat acgacttctt
    11461 aaaataagac aacttcgaat gatgaaaaga atccaacaag ctgaagctgg actaaatgat
    11521 gaagaagtcg aacgattgca gcagctaaga aagattaaaa ctccgattga aaaaaatggt
    11581 aaaaagctag aaatcaagcg tgaggttatg caagatgtgc agattagcag aaaaaaacat
    11641 cgcaaaattg atgatattct ttcaattgaa gattcattga ctcggattag caaccagtta
    11701 gctaaagcca tcaagcaaat gaatgaactt tatatgaatg aatacagaac tgatttaatt
    11761 aaagctcaga ctgataagat ccaagctgag acaaatgaaa ttggcggaaa taattcaggt
    11821 gaagaaatag aagaatggaa acaggcagtt ttaaatgccg caaacaaacg ggcggtgaaa
    11881 gaaaatgaat aatgaattta ttccttttgc tgatattggt tctgccattg attattacta
    11941 tgataaacca gtagcttttt gccaagatat tttgcatttg aatcctgatg aatggcaaga
    12001 aaatgtttta aatgatttag ctgaattttc aaaggtttct gttcgttctg gtcaaggagt
    12061 tggaaaaaca gcattagaag caggagcaat actttggttc ttaacgtgtc gaccctacgc
    12121 taaagtaata gcaacagctc cgacaatgaa gcaactttac gatgtacttt gggcagaggt
    12181 agctaaatgg ttaaatgata gcttgatcaa aaacttactg aagtggacaa agaccaaaat
    12241 ttatatggtt ggtgattcag agcgttggtt tgctacggct agaacagcga ctaaaccaga
    12301 aaatatgcaa ggttttcacg aggaccatat gttgattgtg gtagatgaag cttctggtgt
    12361 gtctgatcca attatggaag ctattcttgg tacgctatca ggttttgata ataagctgtt
    12421 gatgtgtgga aaccctaata atattgaagg tgtcttttac gattcccaca attcagaccg
    12481 tgataaatac agagttcata aagtatcaag ctatgatagt aaacgtacaa acaaagacaa
    12541 tatagaaatg attcttaaaa aatatggaaa agaaagtgat gttgctcgtg tccgtatttt
    12601 tggagaattt cccaaaggtg cgttggattc atttatcagt cttgaaacgg ttgaattggc
    12661 tacagaaaaa caaattagtg attctttagt caataaaaca acggttgctc atattggtgt
    12721 tgacgtagct cgatatggtg atgattctac gattctcttt cctagaattg ctaccagggc
    12781 attggagtat gagaagtatt caaaacgtag cactatggaa acaacaggat atgtcatcaa
    12841 catggccaag aatctaatga gtcaatatcc gagtattgat aaagtgatga ttaaagtcga
    12901 tgacactggt gtcggaggtg gtgtaaccga ccgcctagaa gaacttatag aagacaagca
    12961 ttatcctttt gaggtgtttg gagtgaataa cggttcaaca tcagaagacg atttttacga
    13021 taatttaggt actcaactat ggggaaacat caaggaaatg ttagaagaaa atatgacagc
    13081 aaatcttaac ggagaacagc ctgttattga attgccttct gatagttcgt taatcaaaga
    13141 attaagtact cgcaaattca aaatgacaag tagaagtcgt atacgtttag aaagtaaaga
    13201 tgatatgaaa aagcgaaata ttggtagtcc cgatattgct gacgcactgg ctttagcgtt
    13261 ttatgagcca ccaagtcact atcaatttat tcaattttag gaggtgagtt tttgattagt
    13321 acagtattat ctttagagag atataagaag ctacgtgtta aatatgcaac acaaattgaa
    13381 gatggaatgt ttgatccgaa tggttttata gaatatatga aaccattttt tgctgatcgc
    13441 gaaagaaaat atctagctta tactagcgaa aagaatgaaa tagacaatag accaaagcct
    13501 aacactgata ttgtaaaagt taataataaa cttcatgctg gtatgtattc aattgtcgta
    13561 gaccaagcag tcaatcattt tactggtata cctatcaaat gggattacga tgtatcggaa
    13621 caaaaaagaa cactcattca aagattaaaa gataaattct taaaaaatga tatagaactt
    13681 ccaacagttc cagaagcttt taacaaatta acaagcaatc ttgattccat gagattcgca
    13741 atgcttgatt ctgaaactgc aacttttcaa ggagcttgtg gagtagcttt ccgattgtta
    13801 gagcctgtgg aagaagatga tggttggaag ttaagagcaa gtaatattga accttggaga
    13861 gcagaaagat atggaaatgc cggaatctat atcaaagaaa agtatgactc ttaccagaaa
    13921 aaattttttc aagaaatgaa agttatgaca agaaataaaa ttctgacata tgcttgctat
    13981 ggtgatttga attttgttac aagcggaacg ttcaaaaaaa ttgatgagac cgataaccct
    14041 ttggggacga ttaccttggc agaatttaaa aataatacga atcgttattg tgattttgaa
    14101 gtagctgagg aaattggaga tgcaattgat cgtgctttgt ctgatcaaca aaacgaaatc
    14161 gaacaattta agcttgcgta catgcttgtt actggcacaa caatgagcaa aggcactgct
    14221 aaagaaatga tgaatcagct agggattatc aacttaaaag accctactgc aaaagctgaa
    14281 tatgtcacaa aaaaattagc aaaagatttc aatgaatatc atatggatct attgaaaaaa
    14341 cagttttaca ccatttgtaa agcaatcgat tttaacgatg aggtgtttaa atcaaacagt
    14401 tctggagaag ctcgcaagtg gcaaatcatt agcctagaag cgaaaacaaa tactaaagag
    14461 caatatttta gagaaggatt gaaagaatgc gcagaaacaa ttgctgcttt tcttaagttt
    14521 catgacaaag tagaaattga gcctgaaaaa attattttta ctttctctag atctttgcca
    14581 actgatttaa gttatttagc tgaagcttta cctaaacttg caccatacgt atccaaacga
    14641 actattcaga gccaaattcc atttgtaaca gatgttgatt atgaaaatga aatgatggaa
    14701 ttggaaagtg gaagtgctta tccagatagt gaatacaatt ttggcggagg tggcaatagt
    14761 gacgatagaa acgaagtatt ggaccaaacg tcgagaactg gaggatcaag caaggctcaa
    14821 acaagaaaat cagacactta aaaaattaac tagtgtattt cctgaagcac tgaaagagat
    14881 acaagcaaaa ctattatcac aagctgactt acacaatatc acttatccag aaatgatgga
    14941 gttttacagt acaagtaatc agaaaaaata tcgtgaatat gtggaaaaaa attataagtc
    15001 attaaaaatg tatgatgcaa aatacaaaga gtttatcgat gaattttttc caccatttga
    15061 ctatgcaaaa gtcaatcgct tattacaaat acgatcagat gtatttaaaa ttcttgcaga
    15121 atatgcgatg gatgcagatg tgaatcaata tttttctgat cgcttagagg aaattcttca
    15181 aagaacatat tcttctaatg ctaatgtttt tgttcaactt ttaaacgttg atataccaaa
    15241 ttatttacca gaaaatgaaa ttaaacatta tttgaactat ccatggtcag gaaagacatt
    15301 ttcaagaaga ctttggggaa atatttcatc gcttgaacag aaactctcta atgctattgt
    15361 aaaaagtgtt gctagtggag aaggcgttat acacgcatta aacaccatga gattagactc
    15421 agaaatttgt gacatgttta agttagaaga atcaaagtat aacaaagcga tagaaaatct
    15481 cgttcgaacg gagtatgcaa aatttgcaca agatggtatt gaaaaatcat atttagaaac
    15541 aggtattgag gaatacaacg tattgactgc aaaagatgag agagtttgcc ggatttgtgg
    15601 aggaaaggca agtaagaatc cctataaact gaaagatgct gtcataggtg aaaatcgagc
    15661 acctttccat agtcgttgta gatgtacgga tgttcctaat ttaccaaaat taggaaagga
    15721 tattgatgaa gaatatgacc gtttatttgg cgatttatta gatgagtttg cacatgattc
    15781 ttttggaatt aatttgaaac ggaggaagta gaatgaaaga tttttttgaa gcagtactaa
    15841 caattaatgt aaatgctgat attgcagaag cctacaaaac agctattgag tctgagaacc
    15901 atcctaatgg cttgagagac cattggaatg gcaattatgc ctacgtggtt attggcgatc
    15961 aaactgttaa ttatcaagat aatactccag ttgataagaa taccgttaat ttaacgattc
    16021 aattattatc tcattcatta ccaaatttaa aagaaacagt tgattggtat gaaaagatgg
    16081 gatgtattgt tgttagaact gactacaaag aaggaaagtc tagtaattag gcttttttat
    16141 tttgtccgaa atgacgttaa actagcgcaa tactgggctt ggttgaatgg tggggcgcaa
    16201 ctattaaaac tcaaagcaat gcggggcgtg aaaacgaatc gtggggcgaa aggagaatga
    16261 gcatgaaaca caaatcatta atgccaatga atttgcaata ctttgccgaa ggtgacgatc
    16321 aaaagttttc ttttgatgac ttcaaatctt ttgtggaatc caatgaagaa gctcaaaaat
    16381 ttattcagtc acaatctcaa agtgttgccg acaaacaatt ggaagcttgg aaacaaaata
    16441 acttagataa aattaaacag gataccatca aggaatatga ggaatctaag aaaaacaagt
    16501 cacctgagca aattcaactg gaaaaactac aagctgaatt tgaagcagaa aaagcgttgc
    16561 gtgtgacaag tgataataaa gcatttgttg cagaacaaat tgctggatta gaactagatg
    16621 gagagttaaa agagtctatt tctcaattta tgctaaataa tcttgttagt tcggatacag
    16681 atttcactaa gaatgctgtt gaaggtttta caagtgtttt gaatgcaatt aatgagaaac
    16741 atgcagatgc attaaaagaa ctacaaatga agtctgcatt tggtggaact caacaatcga
    16801 ataaccaggt tcagcagaac aatgaaacat taacaaatcc agaagaacaa ttaggacaaa
    16861 ttcttcaaca atttaactag gagagtgaaa aattatgaaa aaaacatctt taaataattt
    16921 agagtatttg gatatttcac cagcgattaa tgctatgcaa gtaccaaata cacctttttt
    16981 aagctattta cttggtgctg gaaaaacaga gccagcaaac tcgacagaaa ttaaatggcg
    17041 tgaatatgat atcaacaacg atgattcttc tgaaaaactt gagggcggag aatatccaga
    17101 tgctgaatca ggtcgaactt ggtttaacaa ctatactgaa atttttagaa aatcaacctc
    17161 tgtatctggt acattagatg ctattaatgt gaatggagtc ggaaatgaat taactaatca
    17221 agtagcacta cgtggtatgg aaatgaaaat tgatttgaac cgaaaattga ttactggtgt
    17281 aaaagctgat gaaaatggtt ctaaaggtcg tcgaatgaat gggattttga acttgatcaa
    17341 ttcagcaaat aaggcagaaa cagccactgc gggtgcagta acaagaaaag atatcgatac
    17401 tttatttaaa ttgatgtatg aaaaaggtta tatgggagaa aaactatgct tgatttctcc
    17461 agatatgcag gagttaatga ctgatgagtt agatggaaaa tcaacaaaaa ttgttcagtt
    17521 cggggaaaga gtaacttttg gattgcaaat tggaaatatc gtgtctaatt acggtacagg
    17581 tattgctcta ctagaaccat cattgccaaa aggaacaatt gccgcaatcg atactaatta
    17641 tgtgaaacta cgtccattac gtgaatggag agcagaagaa cttgcaaaaa caacggattc
    17701 tagacgtatc ggtcttgtag gtgaatactc tcttgaatac aatgcttcaa actctggggc
    17761 aattttaaat ttaaagtctg aataaaaggg agttagtact cccttttttg ataggaggaa
    17821 ttacagtggt aaaaaaagat gaaactaaaa aagatgaagt cgtgaaatat agagtaggta
    17881 aaactaaaaa ttttgttggg tttgttcatc ctaaaactcg tagatttatc acagcagatt
    17941 caaataacga atttatcatt tctatagatg ataaagaagc aattgcaatt ttggaagatg
    18001 caattgatgt taatgaaatt taggaagtga tctgatggat gaatcgctaa aaacggaaat
    18061 cattgagtct acaaaagaag attttccaga tttgagtgaa gaacgcataa ctaatttatt
    18121 agaaataatt ttgctagaaa ttgaatcata caacacttgt aaaaatgata tttcatggga
    18181 aaagttaaaa agcgtgatta gtgaagtgtt gtataaaata ataaaaaatg aatcagaaaa
    18241 aacagtatct tcagttagac gtggcgatac aacgattagt tatgcttcaa caacaaatga
    18301 tgttagtgag ttacttctag gctatggtga tttgatacga agagttattg gttgtggagg
    18361 attggagttt ttttaatgaa tgaagcagat attttagaaa ctacttatga agatagttgt
    18421 attattgaaa gactgacgga cattgaagat tctaatacaa atattactat tcaagattac
    18481 aaaaaagtat atgataatcc tatttcttgc gctctttcac aaggtcaaat cgatggacta
    18541 gcagtcatag aagatggaga gatggtaaat gtttcaactg acacatataa attatttgtt
    18601 catcctaaga ttaaactaaa aaaaggagat cgaataacaa taactcaaaa ggccagtggc
    18661 ttaatttttt ctctatttgc tactaagcct ttttactatc ctagtcattg tgaagtgaat
    18721 ttgataggaa gtgagaaaaa tgggtaatct caaatatgaa tcaaatgcag aaaagatcat
    18781 tgagaatttt aaaaatatga ctgtaattgc tcaaaaagaa ggaatatcct tcgttaacga
    18841 ttcaatgaat aaagtcgtta gtttagctaa accattaact cctgtaaaat caggtaattt
    18901 aaggcgagga tatcgtgtag taaaagctag aaaactatca agcggtcgaa ttgttggagc
    18961 agttataaac aatgaacatt attttaaata tgtagaagaa ggtcatagga ctaaaaatgg
    19021 tggctttgtc aaagggaaat tcatgttaac tcgtgcaacg aatcttgcaa atatgtctta
    19081 tattcctcga agatttaaac aaatggcaat aaaaatcgtt aagaaaggaa agtaacatga
    19141 aagatgaaat cattgctgca atcagtagca aattaaaaga aatctatcct gatgggacaa
    19201 tatatcttga ttcagttatg cagtcaacta aagattttta tttcgtttta tcagtaatgg
    19261 aatctggaac tgaaaatgta ggaattgata ttcaaaatgt ttctttctta attgatattg
    19321 cattgattga taataaacct aatagaaatt taattaatga attagtctca cgttgtggga
    19381 ctttttttaa tacgattaca atcgatgagc aaatattatt tccaaaagcc tatttacctg
    19441 atgaagcaga tggtgttcaa catattcgtt ttacattaga atttccacaa tatattgaat
    19501 ggagtgaaag ataaatggga gaaaaaagaa gtaaaaccgg aattatttct gtcgaaaaac
    19561 ctacttggtt tcctttggaa gatgagacag ggaattttcc ggtatatggt actgcgttgc
    19621 caatgggaac agcagtaagt atcaaaccaa cagctaatta tgaaacaaca caagattatg
    19681 gggattcagt ggttcaagat caatttacag cttttggtgg tgcagaagtt gaactggaag
    19741 caaatggata tactcataaa gttttatcta caattactgg agggaaaatt gttaaaggtg
    19801 gtgccttgcg ttctggagaa gatattgcgc aagatggtgc gtttgcatat agacgtaaaa
    19861 aatcgaatgg gaaataccgt tatacggtct tttataaagg acagtttgct ctagattcag
    19921 atgaaacatc aacgattgaa ggaagtaaag taagttttac tcatccaact tggaagggtt
    19981 catttgtaga tgtaccagga cttggatata tgtattcagt tgatgaagat gatgaaggcg
    20041 tggataaagc aatgatcgga aattggttta caaaagtagc aattccaatt gaagaagcag
    20101 aactttcagg aggtacagaa taatgtcaaa atatcaaaca acgattaaat taacgaaaaa
    20161 agatgaagaa ggtaaatatg agcaagtaca atttaaatct gctgaatttc taccaggccc
    20221 agtcgtagaa gaagcagcag aagtaatgga gggaatgcaa aatgctgtag atggaaaatc
    20281 agtatcagaa gcattaagtc gagcttattc atttattgca gatattttgt ttgaaggaca
    20341 gtttactggt gaagattatc gtcaagggat cgatgctcga gaaattgctt cgttgacagg
    20401 taagttatta aaatctgtta ctgcaggctt tgatgaaact tatacagaaa cgaaaaaaaa
    20461 gtaagcgaag ctctcaaata tccttttttt aaacattcga ttacttatcg agaattagat
    20521 ttaaagacgc aattactcga agcaggttgg acgttaccag aaattgaacg tactgacttt
    20581 gacgagttaa tgcgtctttt tgcttttcga gatgcagtaa aagagcatga ggatgttgag
    20641 tactacgata acttcaccca attttaggag gtgatacttt gaataacgat gacctaattc
    20701 tgaagatgat actagatgaa tctggtttta ctgccggtat gaataatgct gtcaaaaagt
    20761 tgaactcttt tgatgaaaca atcgaaagaa aaagtagaaa tagtggcagt tcattaggta
    20821 gtatttggaa aatatttgct ggaagttttc tagctagcgg agtaactaga atcgtaggag
    20881 ctggctttga tctaatcaag ggttccataa gtggagcggt tgatcgagta gatacgatga
    20941 ataacgctct acgaaatttc caaaatatgg gatttagtaa ttcagaaatt atgaagaata
    21001 tcggaaagaa tgggctttta tctcaaggta ttcaaggact tcctactgct ttgaatgatg
    21061 cgataagtca tgttcaactt cttgcttctt ctacaggtga tttaactcgt tcaactcaaa
    21121 tatttaaagc tttaaatgat ggaattcttg gtttcggtgg ttcaactgat caagttaatg
    21181 aagctgttat tcaattatct caaagtttct caaacggaaa agtagacgca caaacttgga
    21241 actcaatgat caacgctcaa cttggaccta ctctttctgc tattgctaaa aagatgggaa
    21301 ttacaatggg agatctgaaa gaaggtttat ctcaaggtaa gatttctgtt gaagaattcc
    21361 aaaatcaatt aatagaaatg gataccaaag gtggcggagg acttaaatca ttaagtcaaa
    21421 tcgctaaaga ttctactaag ggaattaaaa cctctataca aaatgcaaaa acagctgtta
    21481 cacgtggtgt tggtgaggta atagaaggat taaataaagc attagtagat tcagatttag
    21541 gtggatttaa gggaattatt gataaagtgg gcagttcaat ggaatcgttc ttaaaagtaa
    21601 ttgctgcaaa tattcctaca gcagtatcat ttttaagtaa tttatttaac gaaattcaaa
    21661 aatttggttc tgctttgaaa ttcatgatgc cctttcttgt tcctgcagct accgcttttg
    21721 gagcgttcat gtttcaactt aaaggtatac cagcaataat aaaaagcttc aataatttta
    21781 agaatgccat aattggcgtt ggaaattcat taaagattat gggggcaata gctgctgcta
    21841 attcatttgt tttaattatt ggtgcagtcg ttggtgcaat agctgttttc ggttacttta
    21901 tggcaaccaa tgaagagctt agaaacaagg ttatatctat ttggaatgat gtgaaagatt
    21961 ccgtaattgg tgtattaaaa aatataaagg actggggaat tgatacttgg aattctgcta
    22021 aagaaatggc atcaaatgca gttgagggtg tcaaagatgc ttggtcagga acaaaggaat
    22081 ggttttctaa cacctggaaa ggtattaagg atggggccac aggtttattt gataaaacag
    22141 tagagacttc aagaaatgca gtcgatagtg taaaaaatgc atggtctaat atgaagaaat
    22201 ggttttctga tacttggcaa agtataaaag attcagccag agaaaaatgg aatggaatca
    22261 aaggttctat tatgtcagtt gctggtccat tgattacagg tataaagaat gcatttttac
    22321 atgttacttt ttatctagag accttatgga ataatttagt tgagattggt aaaaacgtct
    22381 ttgagatttt aaaaaatgtt atacttgcgc cggttttgtt tattacctcc atgatttctg
    22441 gtggatggga agaaacaaaa aataatatga ttgctgtttg gaataatatt aaagaaagtg
    22501 ccataaatat ttgggaatct ataaagaata tatttgtaag ttattttaca aatatttatt
    22561 ttgctgcact caatatttgg acagggttca agcttacatt gataaatatt tggaatgaag
    22621 tggtaaatca agctaaatcg atttggttaa acataaagta tttttttatt aatctttgga
    22681 ttgatataaa atattttgca attcagaaat ggattgaatt gaagttttct attattcaaa
    22741 cttggatcga tttaaaatat aatgccatta ctacttggaa taatattaaa cagtttttca
    22801 aagatacttg gaaaaatatt aaagatacgg catacaatac gtggatttct ataaaaaata
    22861 ccatgattaa tacatggaat aacattaagg actctttctg gaatattgtt actggaattg
    22921 ttaattctgc tgaaaatgca tggacaaatc taaaaaatgg tgtttcaaat gctattaatc
    22981 gggtaaaaga aatctttaat tcgttaaggg aagtcaattt atttgaaatt ggcaagaaca
    23041 tcattgatgg acttataaat ggtgtaaaag aaaagtggaa tgctttgaaa gatactatca
    23101 aaggtattgc aggtaatatc aaaaattgga ttaaaggagc tttagatatt cattctccat
    23161 caagatggat gcgtgatatg gttggtaaaa atattgttca aggtattatc gttggtattg
    23221 ataaagagca aagtaagtta gatcaaacca tgacaaattt agtaaaaaca ccgtctgttc
    23281 aacctgtagt aaatggaata agtcacctac caattacaca aacaaagcaa aatatatctg
    23341 ctagtgaaac aaaagaaatt catttacatt taaatgttta tggagattta cctgattcaa
    23401 tggttcgaaa gattactaca aaaattaaaa cagaattgac aagacaaatg aaacgagatg
    23461 ctggtgcagt aggaggaaca ttatatgcaa cttaagagag gacaattttt tattaatcaa
    23521 cattattctt ctgaatttaa tgtgtatatt caaaacaggc ctgcttctgt ttcagctagt
    23581 cgagtaattg aattgagaga gcgagaagga aatgattcta tcattattga taaaggctat
    23641 tacaaaaatg tgactagaaa gattgagtgt tactataaag caccttcaat tgatatggtg
    23701 caggagtggg aagatcgaat cactgaatgg ttagatatgt cgtcttatag tgattttatt
    23761 ctttactatg atgaacaata tatttatcaa gctgtagtaa tagaaggacc tgaatttaaa
    23821 ggaacaagaa aaacaggaaa tattgtccct tttgaattta cagtgagtat tcgaccattt
    23881 aaggaaaatt acaatggtag atttactatt cgacaaacag aaacttttga aatttataac
    23941 ccagagaagt attcttcaaa accgcttatt aaattgagtg gttctggaga tgcttctttt
    24001 tatattaata aggataaata tgatttgaaa tcgttggaca gagaattatt tatagattct
    24061 aaattagaag aggcttatcg aaaattagat ggtaatttag aacatcagga tcaggtcacc
    24121 ttatttttag attttccatt tttatatcct ggaaaaaatg aaattaaatg gacgaaaaat
    24181 attcattcat ttgaaataat gcctaggtgg tggagaaaag tatgaaacca agaatatata
    24241 gtcctactga aacagatttc tcaacgaatg gcttaggaat tttaaaggat tgtacaagat
    24301 gtgaaatata tgaagtagct aatggaaaat atgaattaga gttggattat cctttaggaa
    24361 ctagatttga tgaatatttt gaaaatgact atcaaataaa agcaaagcca aacgatcaag
    24421 aagagtatca tatctttttt attgatgata aagatataga tactttttta aatacagtaa
    24481 ctatttatgc tcagagtcgt acaaatcgac ttggaagacg ggtagtcact cttgtggaag
    24541 tggactctaa aactggtcaa gaagcgatat caattattga aactaaaatg gataaaaaat
    24601 ctgacatacg actttattct gatattacgg ctgtttctag cacaatcttt gaagcgagaa
    24661 atgttttaaa ttgtattgct ggtgaacaag gatcattgct tcaatattgg ggcggagaaa
    24721 ttaaacgtga accatttaag ctttctttgt taaagcgaag aggtcgcaat aatgttggaa
    24781 cgattcggta tggaaaagat atgtctggtt tgaaggtcaa attagattgg acaggtataa
    24841 aaacaagaat tattccatat gctgatcctc aaagtgaagt aggtacgact aatcgaattt
    24901 atggttcgcc agtagatagt gcctatatta ataactatcc tgatgtctat acagaacatg
    24961 ttcagtttac agaagaacaa ggagtaaaag acattaagag cttaaataaa atagctaaaa
    25021 attactttaa aacgatcaat cctggctgtg ataagcctaa agtttctatt acggttgaat
    25081 ttgataagtt gactgatagt gaagaagcga aagaatttgc gaagattaga aattatggtc
    25141 tgtttgatac atttaaaata tatcataaaa aatataatat ttatcttgaa tcaaaggtta
    25201 gtggactaca atacgattct ttatctgaaa aaactttgaa attagaagca ggagacattc
    25261 aagttgcttt ttatcagcaa caagctgtaa ctattcagga taaattaaaa gattatgcaa
    25321 cgaataatta tatgagtgat tttaatgatt atgtttcttc aatgattaca ggtcaaggca
    25381 gtgcaggagg atatgtagtt ttatggccaa aagaaaaacc ttccaacatc tttattatgg
    25441 ataatccaga tttagaaaag gcaagagaag tactaagaat gaataaaaat gggattgctt
    25501 tttctaagaa aggatggcgg gggcctttta attcagcttg gacattagat agtatattta
    25561 atgccaattt tatacaaact ggattaatca aagcagatat ctttcaaaat tcttttaaca
    25621 agactggaga tgtattgaaa ttagttaatg gactactcca aatttggaac aacaaaaaga
    25681 aaataatgga gttaactaaa aaaggaatgg aattttggaa ttctaagggt tcaattggaa
    25741 cgattggaac aactgattct gttggtaatc cttttcctgg ggcttctact ccaaccccta
    25801 ttgaagataa ttctttagtt attcgtacaa atggagacgg caaatatatt ttgatttctc
    25861 ctaaagtagg aaaaggatta gttttattag gaaatggtaa agcaatttat tttggagact
    25921 tagatgtaca aggaaaactt acagttaaag gaaaagaaat aattcctgga caaggtggtg
    25981 gtccatcggg aggtggagaa tctactggtg ggtatcctag tgaattaaaa acagatgcag
    26041 aaaagagagc ttggagaat
//
LOCUS       DADVXU010000032        25796 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.034, whole genome shotgun sequence.
ACCESSION   DADVXU010000032 DADVXU010000000
VERSION     DADVXU010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 25796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.034"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..121
                     /locus_tag="KB953_001244"
     CDS             <1..121
                     /locus_tag="KB953_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBB6866232.1"
                     /translation="EKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            398..2458
                     /locus_tag="KB953_001245"
     CDS             398..2458
                     /locus_tag="KB953_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HBB6866233.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2541..3110
                     /locus_tag="KB953_001246"
     CDS             2541..3110
                     /locus_tag="KB953_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB6866234.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKSDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3285..4091)
                     /locus_tag="KB953_001247"
     CDS             complement(3285..4091)
                     /locus_tag="KB953_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866235.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            4229..5743
                     /locus_tag="KB953_001248"
     CDS             4229..5743
                     /locus_tag="KB953_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HBB6866236.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(6146..7129)
                     /locus_tag="KB953_001249"
     CDS             complement(6146..7129)
                     /locus_tag="KB953_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HBB6866237.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(7134..7781)
                     /locus_tag="KB953_001250"
     CDS             complement(7134..7781)
                     /locus_tag="KB953_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBB6866238.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7866..8498)
                     /locus_tag="KB953_001251"
     CDS             complement(7866..8498)
                     /locus_tag="KB953_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBB6866239.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8514..9065)
                     /gene="coaC"
                     /locus_tag="KB953_001252"
     CDS             complement(8514..9065)
                     /gene="coaC"
                     /locus_tag="KB953_001252"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HBB6866240.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(9062..9799)
                     /locus_tag="KB953_001253"
     CDS             complement(9062..9799)
                     /locus_tag="KB953_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HBB6866241.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9796..10785)
                     /locus_tag="KB953_001254"
     CDS             complement(9796..10785)
                     /locus_tag="KB953_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBB6866242.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            11043..11585
                     /locus_tag="KB953_001255"
     CDS             11043..11585
                     /locus_tag="KB953_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6866243.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11602..12687
                     /locus_tag="KB953_001256"
     CDS             11602..12687
                     /locus_tag="KB953_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6866244.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12699..13505
                     /locus_tag="KB953_001257"
     CDS             12699..13505
                     /locus_tag="KB953_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6866245.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13506..14333
                     /locus_tag="KB953_001258"
     CDS             13506..14333
                     /locus_tag="KB953_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6866246.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14330..15403
                     /locus_tag="KB953_001259"
     CDS             14330..15403
                     /locus_tag="KB953_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBB6866247.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15831..18527
                     /locus_tag="KB953_001260"
     CDS             15831..18527
                     /locus_tag="KB953_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBB6866248.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            18604..19092
                     /locus_tag="KB953_001261"
     CDS             18604..19092
                     /locus_tag="KB953_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBB6866249.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            19104..19598
                     /locus_tag="KB953_001262"
     CDS             19104..19598
                     /locus_tag="KB953_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBB6866250.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            19619..20428
                     /locus_tag="KB953_001263"
     CDS             19619..20428
                     /locus_tag="KB953_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBB6866251.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            20425..21246
                     /locus_tag="KB953_001264"
     CDS             20425..21246
                     /locus_tag="KB953_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBB6866252.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            21297..23396
                     /locus_tag="KB953_001265"
     CDS             21297..23396
                     /locus_tag="KB953_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HBB6866253.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            23393..24511
                     /locus_tag="KB953_001266"
     CDS             23393..24511
                     /locus_tag="KB953_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HBB6866254.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            24550..25428
                     /locus_tag="KB953_001267"
     CDS             24550..25428
                     /locus_tag="KB953_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBB6866255.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            25574..25771
                     /locus_tag="KB953_001268"
     CDS             25574..25771
                     /locus_tag="KB953_001268"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBB6866256.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
BASE COUNT         8175 a         4481 c         5445 g         7695 t
ORIGIN      
        1 tgaaaaattt aataaagaac agataatttg gaaaaatcca ccgactccag atgactggga
       61 atattttgga aataccatca tcagagaaaa aaatagacat ttaactgaaa aaatcagcta
      121 gtatttatgg atggcagctt tgtctcacat atttattagc tgatttatgt ttttttcaaa
      181 gaaaaggtaa ctagatataa ctagatataa ctagatatat tacttaacaa aattaaaaag
      241 tacataagtt attattccga ttttcttgaa acaataaaaa atcaaaaaaa atactctcct
      301 aatagtccat cttcttttga ttttatttat ataatgaaat catcttatga aaattactac
      361 ttcatgttta gacaagagta gggaggacta agaataaatg tgttcaaaaa gacgttcaaa
      421 acaactaaat aaacacgctt gtcgtttaga taaggaacgg tcgtgttctg aaaaacacta
      481 cgttaagttt atgatgcgca cgatgctgat cagtttatct gtaggtgtct atatgcatgt
      541 acccgttcag gcaacgacac aaaccatcaa ggtcgtacgt acctctgttt ctgagaccac
      601 tgattcccct atcactatta ctcatacttt cactgatgca aatggaaaac agctacttcc
      661 tgcattgact tcctataata aagtcagtta tcctccaacg ataaaccact actggttaat
      721 tcctaacaaa acattatgga catcggaaac cggaagcagt gtgccgcttt ctatcttttc
      781 cgatatgact gatcctacaa aaggtgatca gttaaaacag atcactgata ttttgaatca
      841 tgctttgaca gatataaagg acggtggaag cgcacaagtt tcttatgtat atgaaaaaga
      901 ttattcacag tttgaaagaa agaaaatcga tgttctggca gggacaaaag tttcgatatc
      961 tgatctggta gaaaggctga agaacgcaga tggaacacaa ggagatatgt ccaaagtaat
     1021 ttcaaacaat gggaatatcg tggatacgac taatacagga acatctacta tttccttgtc
     1081 ctattttgat ccaattgctt taaaaacaat gactgcatct gctgtgatcc atgttcgtgc
     1141 tgatcaaaca gctatcaaag gaaaagatct tcacttaaga acaggagaga gttacgcccc
     1201 taaagatctt gttgaatatg taacaaaaag cgatggaact tcaggaaata aagaagaaat
     1261 caaaatgaac gggacagtag atactacaac tccgggagaa tatcccgttg tattaactta
     1321 tatagactcg aaaacatcaa atgtggcaga aacaacagct gtagtttatg tagaagcatc
     1381 tgctgcttca tcgactgtca tcactcattt tatagatagt caaacaaata aagaacttca
     1441 agcagaaaca catatgagta gtgtaaatcg tattcctgat ttgcgcctac ctgctgaatt
     1501 ttctgatcga gtgccaaata tagatttatc gacttatgaa caaaatggga aagtcacttc
     1561 tatttctgat tggatgcaag caaacggatt agacagaaaa agtcactggt cggagatcct
     1621 tgaagtactt cactcagaac tgatgaataa taggaagcaa tcaggagggg aaattacctt
     1681 tacttatgtt tatataccag acaatcgttc attagaaggt catgatctta ttgttcatcc
     1741 aaacgaaaaa ataaacaaag aagagttgat ccaatcagca acaaattcct taggtaaacc
     1801 cgtaccgtta aaggatgtat cggttacaat caatgaagta ccattggagg atggttattt
     1861 gaccaaacaa acaggtgatt ttacgattat gtatagttat catgacccgt attcactcca
     1921 agaactccat gctcagtcac tattacatgt tttaccatta aacaaacaga tagatcaaaa
     1981 agaacaaaaa agacaagaag aaagcttgtc agaaaaaaaa caacaaatgg ctcatgcagt
     2041 ttctccaaca gggaaagtaa aacaaacaag aagcctagag ttagcaagaa tatctagaaa
     2101 aatctcagaa tctaataaaa cgataaggca agaagctcat ttggccccac aagtttctgg
     2161 attggaaaat gaaattttag agcctaaaat aaaacaacaa tcagaaaaag aatttgttgc
     2221 taaacagtcg gaagatcaga aggaatcaga tgaccagaag gaaacgaaga aaaaacgctt
     2281 gtcaactatt gattccaccg atgagcatct gcagccgcaa gtaccacttc ctagtccggg
     2341 aacacccgca cctggtggag gtattccttc ttctgcttta ggagatttca tgcgaggtat
     2401 ggcaggaaca tgggtatacg ctaatcgaaa tgaatttgac gcagatgact ggttctgatt
     2461 tatatctcta tctgttatag tcaaataagt agctgagtca tatgttattt ggcataatgg
     2521 agacgaggag ggatagaaaa atgttagata aaacgttacc ttacgcagaa atctggatga
     2581 caagaccact tcaacaaaat gttccaatca cgcctttagc ggaaggtttt gaatttcgat
     2641 tttatcaaaa agaagatgaa tctgcttggg cggagattga gacttccgtt ggggaatttc
     2701 aaacgacaga tgaagcactt gagtattttc aacaaaactt tgctccttat cctgaagaac
     2761 ttgaaagaag gatgtttttt gtagagacaa aaaaaggaga aaaagtagca acctgtaccg
     2821 cttggcgaaa aaaatcaaga gagcaagtgt atccggtttt tcattggtta gcagtgaaat
     2881 cagattacca aggaaaaggg ctcgcaaaag cattgactgc cagagtcttg caagaatttc
     2941 ctaccctcca tacggacgga ccaatctatc ttcacacaca gacatggagc catcaggcga
     3001 ttgctcttta taaaaaaatg ggttttactt ttattgctga aaatctagac ggcacaccta
     3061 atccagatta tgaaaaagtt atgcaggttt taagtaaatt agataaataa cgaaatggtg
     3121 aaataagtcc tcagtattgg gataaaaaac gaaatagtca ttcctcatat cttacttttt
     3181 tgagttaata ctatcggata actaatggca tgccatacat tcaaaaaaga aggggcagtg
     3241 acaaaagtat tgtcactgcc ccttctcttg ttttttagca tgttttatcg tttgattttt
     3301 ttaggaataa aattcttgat gaacgtaaat ttttggtaat cgtttcgact atagaggtaa
     3361 ccaaatacac tgaaaacaac tgctccaata aaattaacaa ataaatcctt catagtatcc
     3421 atgattccca cgtctaagta accattttgg atgattgttt tatctgtttt accatctgca
     3481 ttttgacttt caatgattgt ccgagtgata tctggaacat gatagacagt gtttccgtct
     3541 ttacttaact ccacactatt gatttgctgg atgatcgtat ctttttgcat atcgaattgc
     3601 agtaatttat ctgctgaaaa ttccacaaac tcccacataa caccaactgt catcgaaaaa
     3661 cagaaggaaa caactgctag taataaagga gtcaagttca tggtttttgt atttttattg
     3721 agcaaataaa caagggaaaa gccgacaccc ccagctaaaa atccgctgaa tgcatgtaga
     3781 aaagtatccc acattgaaaa atagccataa aaatcactta actctcctaa aactgttgaa
     3841 ctaaaaataa aagataacaa aatgatttct agcatatttg gaatctgtat ctttaatatc
     3901 ttttccagaa aataagggat ttcaaataaa attaatgtca gtaataaaag cattgtattt
     3961 ccccagtgtt ggttccagac ctgatccact agcataataa cgacgatgat acgtaataaa
     4021 atatagattc cgtttgtgat tctacctgca gtttttgtac tgttcgtaaa aaaggttttc
     4081 aaatgattca taaaataact ccttcatgta tatccttaat tatactaaga agcgtgtgtt
     4141 taccaaagct atatgactta aagaaaaaca gagttgatat tgtgaaaaaa atcacttaat
     4201 atagtataag gagaaaaggg ggatacaaat gaaaaaatta gttgctggtt tattaacagc
     4261 cgttgcactt cttgctatga gcgcatgcgg cgcagatcag aaaaatacag cgcagacaag
     4321 ttctagtacg aaggaatctg ccgaagtatc atcaggcgct tctgaacaag tatatacaga
     4381 tccaaaagaa atgaaagaat cttatgatgt cgtgattatt ggagctggtg gtgcagggat
     4441 ggcagcagct atcgaagcaa aagatgctgg gttgaatcca gtaattttgg aaaagatgcc
     4501 tgttgccgga ggaaacacat ctaaatcatc aagcggtatg aatgcttcag aaacaaaatt
     4561 tcaaaaagaa gaaggaatca aagactcgaa tgatgccttc tatgaagaaa cgctaaaagg
     4621 tggtggagga acgaatgata aagaaatgct tcgatacttt gtcgatcact ctgctgatgc
     4681 ggtcgattgg ctagatcaaa acggtatcaa actggacaac ttaacgatca ctggagggat
     4741 gagtgaaaag aggacacatc gtccgtctga tggctcggct attggtggat acttagtgga
     4801 ggggttattg aataacgtcc atgagcggga aatccctatt tttgtcaacg cagatgtgac
     4861 agacattaag aaaaatgggg aaacggtcaa tgaagtgact gtacatgttc aaggagaaga
     4921 accaaaagag gtcaccggaa aagcaatcgt agttaccaca ggcggttttg gtgccagcaa
     4981 agaatttatt gaaaaatatc gtcccgattt agctgattat attacgacaa atcaagaagg
     5041 atcaactgga gatggtatca ccatgattga aaaattaggt ggacaaaccg ttgatatgga
     5101 taaaatacaa attcatccaa ctgtgcagca ggatgagggg gtcttgattg gtgaggttgt
     5161 tcgcggagaa ggagcaatct tggtcgatca agaggggaaa agatttgtca atgaaatgga
     5221 tacaagggat aaagtatctg cagcaattac agcattgcct gaaaaatcag cttatttgat
     5281 ttttgaccaa ggcgtgcgtg atcgtgccac agctattgaa ttttatgacc aaaaaggata
     5341 tgtcacaact ggaaagacaa tggaagaatt agcagaaaaa atcgatttac caaaagaaac
     5401 actagcaaaa acagttgatg gatggaatac ggatgttgcg gagaaaaaag atgctcaatt
     5461 caaccgtgcc acagccatgg aacatgactt atcgtctccg aattattatg cgatcaaaat
     5521 cgcaccagga atccattaca cgatgggcgg tgtgaaaatc aatacgaaca ctgaagtatt
     5581 agataaagaa aaccagccaa ttagggggtt atatgctgct ggtgaagtca caggtggctt
     5641 acatggaaac aaccggatcg gtggaaattc agttgcggag atcattattt ttgggcgaca
     5701 agcgggcata caagcagctc gatttgcttc ggaaaatgaa tgaaatagaa cgtaaataaa
     5761 aagaggttgt gaaatcagcg ttttgagcta ataccgaggg tcagctgatt gaacactgtt
     5821 tagtgaagag gttgtgaaaa agctgccctg catcaagtaa taagaacagc caagtaaaaa
     5881 cagcttctta tgtttttaac ttggttggac ttattaccgc agatgcaagc ttttgaacac
     5941 cgtttattcg gaaaaagagc gtgggacaaa agtaaagaac acttttgtct cacgctctaa
     6001 acacgtataa acggcggaag cagaagaaat tccttcggaa ataagctgaa attcacaaaa
     6061 atttgaaaag caattttcgt gaatttcttc ttatttcttg gagttaaacg cttctgtccc
     6121 agcctctttt ttgatcagca tgattttatt cagttatctt caaaatgatt tttccgatgt
     6181 ttttgtttgc ttccatatat tcatgcgctt gtttgactgc gctgaattgg aatgtacggt
     6241 caatgattgg ccgaatcttg ccattttcta gaaaaggccc agctttttgc ataaaagttt
     6301 gtgcgagctc tgctttatac tgatcacttc taggtgtcaa taatgtacca agcagttgga
     6361 cacgtttccc aatcaataca ccaaggtcta tctcaggcac gatggtccca cctagcatac
     6421 caatcaagac ccaacgtcca tcaacggcta tagaagctaa attcttttcc cagtaagaag
     6481 cgccgatgaa atccaaaata acatcgactc cccgaccttc agtgattttt cgtacctctt
     6541 cttgaaagtc ttgtttttta tagttgatca attcatctgc accaagttct ttacaaaagg
     6601 cgagtttttc ttctgatcct gcagtaacga tgattttcgc gtgagaaagc tgtctagcta
     6661 attggatcgc tgctgttccc acaccacttg ctcctgcatg gatcaagaca gtttcgcctt
     6721 cttgtaattt tccaagccag taaagtgttt gataagcagt caaaaaaact tctgagattc
     6781 cagcagcaga aacatagtct agcgtatcag ggagaaggat tgcacgatcg gcaggcatca
     6841 cagcatattc ggcataacca cctagattga ctaatccgta tacttttgtc ccaggagtaa
     6901 aagtggagat atcactttta ttctcaacta cctctccagc tacttcgact cccaaaatag
     6961 ggtagggagg tttcaaagag actgattctc ttttcataat atctgttcga ttgacagctg
     7021 ctgtatggac tttgatcagt aattcgcctt gttttggttt aggaacaggc acttcaattt
     7081 gttctaatac atctacagaa ccaggatgct tgattgaaat ggcacgcatg gatctattcc
     7141 tcctttgccc attcttggaa tgctgtattc aactctttgc taccttcaga aagtactaga
     7201 gtcgtacgag aaatatccgt ttcttccact agagcggcaa gtacagcagc aactgtagag
     7261 cgagtgatcg tttttgatgc cttctcatta ggcttagtta attgaatgga agtaacttct
     7321 tcatcgttag tcaatgtgac tggttgaaga atgacaaaat ctaagttagt tcgtttcagc
     7381 cattcatctg cataatgttt agtaatataa taatctgtca tggactcagg ccattttgct
     7441 ggttcatctg cataaacagc actgaccatc agatagcgag agacatgcgc attttcagct
     7501 gcgatcactg ttttgattgc gccatccaag tctacttgca gaagattctt tccttgagag
     7561 ccggcagcaa aaatcaatac atcgatgcct ttgaatgctt cggtcatttt ttccacggac
     7621 caagttaaat caaaagagac atacgaaaca ttctcttttt ttactaatgt ttgggaagca
     7681 acatctctca cccctgcaaa aatttcatgg gaacttggag ctaaatcttt tatcaggtgt
     7741 cggccaattt gtccgtttgc acctacaaca aaaattttca tttggatcat cctttcgacg
     7801 tcaaatcttt ccattcaata gtaacaccaa aagagaaaaa aatcagagat cggctacgat
     7861 gactatcagt tagtaagaga agggtgcttg aagtatctta acaagacatt gcatactgct
     7921 gcagtcgcaa gacctgcgac gattccttca ggtataccac ttgtaaaaat aaccgctaga
     7981 atagctgaat aaacagcatt taaatttgtt ccgatgactt tggcataatc ttgctggaat
     8041 aaaaagtaga tcatattcat cactaaaatc gtattggtca atgaaccgga aagtccagct
     8101 aagaataaag aaacggaatc tgctttttgt ttcattagtt tgcgcagacc tttataaacg
     8161 aaataaggaa ccaccccaat caagatgcga ggaataaacg cgatcagcaa ggctttccag
     8221 cttccatgtt ctgttccgat gactgggata aacggagaaa agacaaaaga caaaggtgtt
     8281 tggataactg tgctacggat catgctgatc agtccaaatg taccgccaag aaaagcacct
     8341 agtcttggtc ctaaaacgat cgaagcaatg attacaggaa tatgcatggt tgttgcattg
     8401 attggaccga tagggataaa acctaaaggc gtaacagcta ataaaatcaa aatagctaaa
     8461 aacatcgcag ttaatgtaaa gttttttgta tttttcataa gtactccttt tttctaagca
     8521 tttctttcat taagagtcag catgacctgt tcgatgatcg tctgaatatc agctaaggca
     8581 ccttttccaa aatctccaca agctaaaaga gattctcttg gttcaatttc ctgatagccg
     8641 acagatctca atgtttccag attacgctga gtgatcgggt tttgatacat attcgtgttc
     8701 atggcagggg caatcaattt tggtgttccc actggaagcg ccattgcgac tgttgaaaga
     8761 agatcgtctg caatgccgtt tgctagcttg ccgataatgt ttgctgtagc aggcgcgatg
     8821 agaaacaagt cagcttgttt tgctagttcg atatgattga ttacctgcgg atttgattct
     8881 tgcatgacgt ctgtatggac tgcgcgcttc gacagagatt gcaaggttaa tggcgtgata
     8941 aaatgtgtgc tgcttttaga cataatcaca tctacctggg cacctaattt gacgaattga
     9001 cttgtaagat cagcgctttt gtaagcagaa atacttccag taacgcctag taagatcttt
     9061 ttcattttga ttcctccagt ctaattttct cagtaatcaa tgcagcaatc tcagatttac
     9121 tttgagcttc ttccactgtt ccatctttag ataagagata gccgtggtgt gtctcgccat
     9181 ggatttccgt caaatcattt gcgaaaataa aatcagtttt gttatttatc agactatttt
     9241 ttgcgactgc cagaagagat tcctttggta catctactaa tagtttgaaa ccaatgattt
     9301 ttgcttttgg ctgccattca cgtagataag aaatgatttt tggtgttttc ttcaaaacga
     9361 gcacaagatg ctcggtgtta gaagagatct ttgaaacagt ggtatctgtc tgttcatttt
     9421 ccgcaaacca attgtccagt tcttcttgcg ttgaagaagt gggtaggttt tttgccagct
     9481 gttcttctga aaaactaaaa gcaggagtaa aatcactgac tgccatactg tggataatcg
     9541 catcgtagtg ttcttttttc gttagttctt ctaattgtaa aaataagtcc agtgcagatt
     9601 cgatcgtata aatcgttata ttggagtgtc ttttcggttt cagtgcttgt ttcgtagtca
     9661 cgtaatcgat tgtggtatcc ggattggcag ctaaataatc agctaatgct tgccctaatc
     9721 gtcctgtcga atgatttgta atagcacgta cttgatcgat tttttcagtc gttcctccag
     9781 ctgtgattag aatcttcatt tatggtcacc tacatttact agtgttcttc cttggtgtgt
     9841 tccagcaagt agtttagatg ctgtttcagg caattgatcc agagtaatct cttggacgac
     9901 cagttcatct gcaatctgca aatccgccat ctgttgccat aaagaaagac gttgatctat
     9961 tggtacattg acagaatcaa ttccaatcaa ctggatgttt cgcagaataa aaggcaagac
    10021 agtcgcgttt agtttgattc ccccagcatt gccacataaa gctactgcac cgttataaga
    10081 gatcaatgga agaagactgc ttagctgctc gccacctact gtgtcgatga catagtcgat
    10141 ttgctgtttt cctaaagctt ttgttgtctc tggcagaaat tcatcaggtc gtacgacttg
    10201 caaagcacct agacttttta gccatgtgac agactcttct tttctggaaa aggcaatgat
    10261 tgactggaat cctagtttat gtaacagggc tatcgctgta ctgcctacac cgccagaagc
    10321 accagtaacg acaacttttt tatcggaggt cataccttga cggattaaag cgttcacaca
    10381 caacatagca gtaaaccctg ctgtaccaag gatcatcgct tgacgcagcg tcatattttt
    10441 tggtagagga actaaccagt ctcctggtac tttttgatat tggctataac cgcctggatg
    10501 ggtgacgcca agcccaaaac cagtagcaag tacaagatct tcttttgaaa aacgtggatt
    10561 gttactttgg acgacagttc cggataaatc aatccctgga gtcatcggat atgttcgtat
    10621 cacctgacca gactcgctca ttgccaatgc atctttataa ttgacatcag aataggcaat
    10681 ttttaccaag acttcatttt cagccaactc agtaaatggc agatcactga ttgctgtatg
    10741 gaaaacaggt tcctttatta cctgaaaaga tcgaaaagcg ttcattcgtg agacactcct
    10801 tttaaaattt tcttttatca taccataaag cctactcttt tattcttgtt cttccgtaaa
    10861 tttagacaaa aattaggtga aaaataaaga atcaaacaac tagaatttat tccaaaagat
    10921 aaagaaatat tcagcaaaaa aaattttaca aagggttgcc tttttcaata aatttcgtat
    10981 actaatacga gtggtagtaa acaggaagtt tactattaga aaacttcgat tcggaggaaa
    11041 ttatggaaat tggtgagaag ttaagaaatt tgcgcatcca aaagaatttg acacaagaag
    11101 aactaggcga acgcacagat ttgtcaaaag ggtatatttc ccagttggaa cgagatttaa
    11161 gttcaccatc aatggaaaca ttcttttcta ttcttgaagt cttaggtgtg actccagaag
    11221 aattttttca tcaagaagca gcgaatctgc aagtggttta ttcaaaagaa gatcatacgg
    11281 tttatttcga tgaagaaaac gggtatgaat tggaatggct cgtggctgat tcgaatgaaa
    11341 aagaaatgga gcctgtcttg ctgacttttg aaggtgcagg agaatataag acgtttgaac
    11401 cttcattatc tgaaacgttt atttttgtat tagaagggga attagagtta agcttaggag
    11461 agacggtcta tgtggcaaaa gcaggacagt cgatctatta tcaagcgaca aaacaacatc
    11521 aattaaggaa tcatctcaaa aaacgaacaa aagtattgat tgttgcaacg caatcatatt
    11581 tgtaatatag ggggtaaaaa cgtggggaaa ccaatcattt catttgatca agtagtgaaa
    11641 cgctatgatg atgaaacagt attaaaaaaa gtaagttttg agatcgaaca aggaaaattt
    11701 tatacactgc ttggtccttc cggctgtggt aaaacaacga tcctacgtat tatagctgga
    11761 ttcaccgaag ctacagaagg ggacatctat tttgaaggga aacggttgaa tgatttgcca
    11821 gctaataagc gacaagtaaa tacagtcttc caagattatg cactttttcc tcatatgaat
    11881 gtttttgata acgttgcttt tggattgaag atcaagaaaa tgtccaaatc agatatcgaa
    11941 aagaaggtaa aagatgcttt gcgtatggtc cagcttcctg gatacgaaaa acgggagatt
    12001 agtgaaatgt ctggtggtca aagacagcgt gtagcgattg cacgagcaat tgtaaatgaa
    12061 ccgaaagtat tgctattaga tgaaccgtta tcggcgcttg atttgaaact acgtacagat
    12121 atgcagtatg aactgcggga tctgcaacaa cgattaggaa tcacgtttat ttttgttact
    12181 catgaccaag aagaagcgtt ggcaatgagt gatgagattt ttgtcatgaa taaagggaaa
    12241 atcgtccaaa gtggtacacc cgtagatatt tatgatgagc caatcaatca tttcgttgct
    12301 gattttgttg gggagagcaa tatcgtggac ggtgtaatga tcgaagataa tctagttgag
    12361 tttgttggta agcaatttga atgtgtcgat ggcgggatgc gtctaaatga accagttgaa
    12421 gttgttttac gcccagagga tttgacgatc acgacaccag ataaaggaaa actagtcgta
    12481 acggtcgata ctcagctgtt tcgtggtgtc cattatgaaa tcatctgtta tgatgaacag
    12541 caaaatgaat ggatggtcca ttcaacgaaa aaagctaaag aaggaagcaa agtaggattg
    12601 gcctttgagc cagaagatat ccatgtcatg cgtttcaacg agtctgaaga agaattcgat
    12661 gcacgtcttg acagctacga agaatagggg ggacaagaat gaaaacaatg cgcaggatct
    12721 attccgtccc ttatattttg tggctcggtt tattcgtgat cgcgcctgta ttaatgatca
    12781 tttatcaatc tttttttgat atgaatgggc aattcacgct gaataattat ctatcttatt
    12841 ttacgtcggg gacatcgctt tctatgacga tcaattctgt atggtatgca tttttgatca
    12901 cgtttttcac gctgatcatc agttacccga ctgcttattt tttaacaaaa ctgaagcaca
    12961 agcaattatg gctgatgctg attattttgc ctacatgggt gaatctgctg cttaaagctt
    13021 acgcatttat tgggattttc agtgtgcacg gcagcgtgaa tcatttcttt gattttatcg
    13081 ggatcgggcc gaaacagata ttattcacag acttcagttt tctgttcgtg gcaacgtata
    13141 tcgagattcc atttatgatt atgccaattt tcaatgcatt ggaagagatg aatccttctt
    13201 tgatcagtgc tagtagagat ttaggcgcga atagctttga gaccttccga cgagtgattt
    13261 ttccgttatc attaaatggt gtgaaaagtg gtgtccaagc agtatttatt ccttctcttt
    13321 ctttattcat gctgactcgt ctgattggcg gaaatcgggt gatcacttta ggaacggcta
    13381 tcgaacagca tttcttggtt acacaaaact gggggatggg ttctacaatc ggtgtgattt
    13441 taatcattgc tatgttcatc gtgatgctcc tgacaggcga gaagaaaaaa ggaggtcgct
    13501 cataaatgaa aaaatttaaa tggtcgtatc tttatttagg gatcgtcttt ttattcctat
    13561 atgcaccgat cttctatctg attttttatt ccttcaatga aggaggaacg atgaacagct
    13621 ttacaggatt tacttgggaa aattatgaag ctgtttttga agatacacgt ttgatcatta
    13681 tcgtattgaa tacgttcatg ctggctttct tatctgcatt gatcgccacg atcatcggga
    13741 catttggggc tatgggaatc tattacacga aaaaacgacg tgcacggaca gcgttgttga
    13801 gcatgaataa catcttatta gtctctcctg atgttattat cggtgctagt ttcttgattt
    13861 tctttacact actaggattc ggattaggtt ttcagagtgt attgctttcc catattgctt
    13921 tcagcattcc aatcgtcgtc ttgatggtct tgccaaaatt gcaggaaatg aacgattcaa
    13981 tggtcgatgc ggctagagac cttggtgcta ataatttcca agtagtcaaa aatatcattt
    14041 tgccttatct ttctccagga atcatagctg gatattttat ggcatttacc tattctcttg
    14101 atgattttgc ggttacattt ttcgtcacag gaaatggctt tacgacgctc tcagtagaga
    14161 tctattctcg tgcaagacaa ggaatcagtt tagaaatcaa tgcgctaagt gcgttagttt
    14221 tcttgttctc catgatattg gtcattggat actactttat tagtaaagaa aaccgtccaa
    14281 aacggctgaa gaaaaacaga agagtacgcg agggggtggc acggctgcga tgaaaaaatt
    14341 acaatctctg tttttaggta ttctcatcat tattgcggtg ctgctgcttg gtgttcgtca
    14401 acttgaaaaa acaagtggca tgagtggagc aaaggtactt accatctata actggggaga
    14461 ttatatcgat cctagtttga tcacgaaatt tgaaaaagaa tatggataca aagtgaatta
    14521 tgaaacattt gattctaatg aagcgatgtt cacaaaaatc cagcaaggtg ggacaaacta
    14581 cgacatcgct ataccaagtg aatatatgat ccaaaaaatg atcaaagaaa aactagtact
    14641 tccattagat cattcgaaga tcaaaggatt aaataatatt gatccacgat ttttagactt
    14701 ggattttgat ccagataatc agtattcgat tccatatttt tggggaactt tagggatcgt
    14761 atataacgat aaattctttg atgcaaatca aattaagcat tgggatgatt tgtggaagcc
    14821 ggaattaaaa gacagtttga tgctgattga cggtgcacga gaagttatgg ggctttcttt
    14881 gaatagttta ggctattctt tgaatagtaa gaatatggca cagctgacag aagcagcaaa
    14941 taaattgaat catttaacac caaatgtcaa agcaatcgtg gcagatgaga tcaaaatgta
    15001 tatgatcaat gaagaagcct ccgtggcagt gacattttct ggcgaagctg ccgatatgat
    15061 gtgggaaaat gaacatttac attatgtgat cccaccagaa ggatccaatc tttggtttga
    15121 taatatcgtt atgccaaaaa ccgtgaaaaa taaagaaggt gcctatgatt tcatcaactt
    15181 tatgctagaa ccggaaaatg cggcacaaaa tgcagagtat attggttatt ctacaccaaa
    15241 tgaagaagca aaagcgttat tgccaaaaga catttcgtcg gacgaacaat tctatccatc
    15301 tgatgacacg atctcccatc ttgaagtcta caaagatcta ggatcgaaat acctaggcat
    15361 ctataatgac ctattcttag aattcaagat gtataggaga taatggcaaa actcagtaag
    15421 aatggggact tatcaacgtt ctaaagagct aaaaatcgtc ctttttctat ctgagttttc
    15481 caatcttcta ccaaaagcgg atgaaacgcc gctttttttg tgttatttta ttttttctac
    15541 cacgtttttc taccagagaa aagtggacaa aaccattgta aaatagggcc cgagacgggc
    15601 tagtaaatct aagattgaaa aagttacaga aggataaagt aattggaaac tatgcaaata
    15661 tacttaaccc ttctgtaatc tattaataaa actaatatat gaatccagtt caatatttgt
    15721 agctatgaaa aaacataaaa atcatattag ataaaaacta aattggtaga taaattattt
    15781 tgtcagatat ctgaggcaaa tattcgacta tttgtgtttc aaagtaaaaa ttggaataca
    15841 aatatagaat agacatattg cagccgatag tttattatgc ttttagacac attaaactgg
    15901 aggaaaaaat gaaaaagagc gctaaagatc gagtttttga tgagctgata cgactaacag
    15961 ggaatatgtc ttctgtcact gccgagaaaa tttcgaagaa tctaggtatc tctcgtcaga
    16021 atgccagcca ttatttgacg agattagtgg aagataaaaa ggtcgagaaa ttaccgggaa
    16081 agcctgtttt gtggaagcct ttagatgaat atgcggttgt agataatacg gagcaaataa
    16141 acgaagcctt tcattctgta gttggtcatg acggaagtct gagagaggtc attcaaaaat
    16201 gtatagccgc tgtcaaatat ccgcctaacg gactcagtgt attaataaat ggagccacgg
    16261 gtgttggaaa aagtttttta gccacaaaaa tttttgaata cgctatccat gagcaaatta
    16321 ttgaaaaaga tgcaccattt gctatcttaa actgtgccga ttatgcggac aatcccgaac
    16381 tgctttcggc aacactgttt ggatacaaga agggggcctt tacgggggct gaaaaggata
    16441 ccgatggttt attggcaacg gccaacaacg gctatctttt tctcgatgag gtccatcgcc
    16501 tatcgaaaga aaatcaagaa aagctctttc tatttattga tactggaaac tatcgtccag
    16561 tcggtgaaaa tgtaaattgg cattctgcta aggttcgttt tattttcgcc acgacggaaa
    16621 aaggtgaaaa ttatttatta gacacctttg atcggcgtat tcaaattagt gttatgttgc
    16681 caacttttga agatcgcccc attcgagaac gattagagct gattcaatta ttttttcaaa
    16741 atgaagcgga tgttttacaa aaagatatta ttgtttcaag agaagccttg tctttaatgt
    16801 tatctcatcc ctttagtggg aatattggta agctgaagaa tatcattaaa atcagttgtg
    16861 cggatgtgta tagtcgaaca caagaagaac cattaacgat tgggaaaaat gagatcttga
    16921 ttcagttaaa tatgctggaa tccgaagtag aaagcttgcc gattgctagt ttattgataa
    16981 agaaaaatca aaagataaaa acggatcgaa tcaaaccgtt tgattatgat tcgattttgg
    17041 aaaaaattac tcagctgata aatgaaggta atttcaatga aaatttttct tcgataaaga
    17101 atgaagtaca gcatttggtg catcacattg ataaggattc ttttttatca atgagtagaa
    17161 aattgatgat tcagatgttt agaaaggtct gggtatccgt tttaggaaaa acgtacggat
    17221 tgacgacgag tggacctgtt gcagaaatta ttagcagatc ctatgtcatt tccccaagct
    17281 ataagacaga cttaaccggg ataaatcgta cattagctgc aaatctacct cggacgactt
    17341 acttatgtaa tcagttcttg gagcatttac caccgctctc agaaaaggac gagaactttc
    17401 taagactgct tattgtcgtg actttatctg attatgtaga tgaatccatc gagctgaagg
    17461 gcctgttgct tgctcacgga gatagtacgg ctagcagcat tcaagcagta gtgaatcaat
    17521 tatgcggcaa ttatgttttt gaagcgttgg atatgccagt agagagtaat gtgtcagaga
    17581 ttgtcgaatc gacaaggaat ttcgtgaaac ggcaagtgaa tacagatagt ctagtgctga
    17641 tcgtagatat gggctcttta aatcaaattt acacacagat caagaacgat ttaaaagggg
    17701 agttacttct tctgaataat ttgacgactt ccattgcact tgatattggc ttgaagatga
    17761 cgatgaatgc gccatttaaa cagattgcgg aggatgcagc agacaagtat tcaatcaatg
    17821 ttcaatactt tgaaggcttt tctcaaagta gcaacattat catttcctgt atgtcgggtt
    17881 tggggatttc ggacaaattg aaagatgttt ttcgtcatca tttatcggat gaatccatcg
    17941 aagttttcac gaaggactac agagaactac gtgaattaat cgaccaaaat gatgaaactt
    18001 attttgacaa gacaaaattg gtcatcacga cttcggatct tccacgttcc ttttcaattc
    18061 ctaatattaa tgtttatgac atcttagacg gtgatggagc agccaatttg gagcatattt
    18121 tgagtcctga aatgaagccg gcagaattta accgcttgat tcaggagcta gtaaaattct
    18181 tttcgttgga aggaattgcg gaccgtttga actttctgaa cccaacgatc gtgatgagtg
    18241 aagtggaaac agtcatttct aaatatgaaa attactatca tttcacttta aatggccgtg
    18301 taaaactaaa tctctatatg catacggcat tgatgatgga acgtttattc ttggctcgga
    18361 cagaaaaatc tgaagaaccg aattatgatt tgaaaccaga tgaagatgaa ttttattatg
    18421 tttctagaag tatttttcaa tcaatggaga tgaaatataa ttttaaagtc aatgcttatg
    18481 agctttcgct gctctatgaa ttattaggac cgtatattca aaaataaagt gtctaaaaaa
    18541 gtgtctaatc tgtttttttt aggttagaca ctttttttaa tgtgaattaa cgtgattaaa
    18601 ccaatgtttt taaaagttgg cacaggaatt gcttatataa aagtgaacag aaagaaagga
    18661 attaaccata tgaagaaaat tctaatagcg actcacggtc acttagcttc aggctttctt
    18721 agcagtattc aattactgac ggggaaggtt caagagatta cggtgattaa tgcttatgtg
    18781 gatgagtgtg attttgaaca ggagttgaat gtatttgtaa accaggtgaa cgagcaggat
    18841 cagatttttg tttttaccga tttatttggc ggaagtgtca atcagaagat taccaaaacc
    18901 tttttggaga aagagattgc tgtaactatt ttgtctggct ttaatctgcc aattctgctc
    18961 gaaatagtat tggcaaccaa ggaattaaca aaggaggata ttcaggaact ggtaaataaa
    19021 tgccaacagg aattaaagat gaccattctt gatgaggtac atgtagtaaa tgaagaggaa
    19081 gatttttttt aggaggaaga aaaatgatta aagcagtgcg aattgatgaa cgattaattc
    19141 atggacaagt agcgatgact tggacaaaga gtctaaattt aaccgggtta gtagtagcaa
    19201 gcgatgaggc ggctaccaac gatattcaaa agatgacatt gaagatggct gcaccatcga
    19261 atgtcaaagt cattattaaa acagttgatg acgcgattca tttgttaaaa gatccgcggg
    19321 cgaaggacat gcgcttgatg gttttagtcc ctaatgtcaa agatgcggta aaagttgcga
    19381 aagctttccc aacggaaatc gaattaatga acgtggggaa tgctgggaaa atgacaccgc
    19441 catcggaaga taagaaaacc ttatcaaagg aagtgatgtt aacgacgtca gaaattcaag
    19501 acttaaaaga attagttgaa ctttatccag ataccgtttt tcagcctaca ccggcgatgg
    19561 aaaaacgttc agctatctct attttgagtg atttttagaa aaaggaagga gaaaaattat
    19621 gtatgatgca tttgtagtcg ctttggccgt ttttattgga gttgctggac atgaaatgtt
    19681 cgggatgtcc atgctgagtc gtccgattgt tgttgctccg ttagcaggag cgttgatggg
    19741 ggatttgcag acggggttga ttgtcggggc gtcactagaa gccatcttta tgggtgtcgt
    19801 caatatcgga atctctagta cggcggagcc ttctttggct gctgggctgg ctacagcgtt
    19861 cgcaatcaaa atgggaggaa acgtggatgc gattattccc attgtctttc cactagcagt
    19921 attaggatta cagttaatga atatgatttt ttcttttgta tgtggaccga tggctgctaa
    19981 atttttcaaa tttgcaaaat ctggtgatca aaaaggaatc atcaatctgc acttctttat
    20041 gtggtttgtc cactatgcgc tttatgctct tattccattc tttgccgttt tatttggagc
    20101 agatgttgtc aaaagcgtct tggataatat tccagaggtc atcatgaacg gattaacagt
    20161 cgctggaaat ctgctgccgg cagttgggat ggcgatgctg ttacggatgc tttggagtaa
    20221 aaatattgct gtgtggtttt tctttggggc cgttgttatg gcgtatctcg agttaccatt
    20281 gattgcaatt gccgcagttg gaacgattat tgcaattgtt atagcccaaa gagatctaca
    20341 actaaaaaat atatctgttc atgcagttca aagcgatgct gctgtaaatg caacgcttga
    20401 ccaagaagag gaggatttct ttcaatgaaa ttagctgatg gaatttctaa agatgagaaa
    20461 aaattggttc ggaagatgtt ttggcgctcg atgactatgt atgcttcggt gaatccggtg
    20521 acgatgggcg gcggtggttt cgcctattct atgatgccct tcattaatca tttctacaaa
    20581 agtgaagaag atcgagctgc ggcgctggaa cgtcatacac agtatttttc gacgaccatt
    20641 ccttgcgcgt cctttgtcat ggggattgcg gcatcgatgg agaaggaaaa ttctgaaaaa
    20701 gctggttttg acgtggaaag tatcaacgcg atcaaaacga gtttgatggg accattggct
    20761 ggaattggcg attcattgtt ctggggagtt tggcgtgtcg ttaccgcggg actagcgatc
    20821 aacttagcca atgccggaaa tattttggca ccaattatct ttttagttat gttcaatata
    20881 cctaattact tgatgcgata ctatggcggc gttttaggct attcattagg ctctaaatat
    20941 attgaaaagc tgtatgcgaa cgggttaatt gaaattctga caaaagctgc tagtatcttg
    21001 ggtctgatca tggtaggtgc aatgacgatc agtatggtca aattcaatac cgttttgagc
    21061 ttttcaatga agggcgaaga actaatgaat gtccaagaaa tcctcgatag tatttttaaa
    21121 ggaattgtac cgttattggt aacattgagc tgtttcaaat tactagataa aaaagtcggc
    21181 gtcatcaaaa ttatttttgg aatggtcgtc ttgggaattt tattgtcatt acttggaatc
    21241 gcatagtagg aagctgcagc tactaggctg cagttttttt agcaaggagg gaaaagatga
    21301 ctattaatga agtaatttat tcacccaaac acccattttt taatgctgag ttaaacgacg
    21361 gaaatgtgac tacttcaaac gaatacagct actatcggaa tattcctgat gtaaagggat
    21421 tgaccttcga tgtaagtaat caaaagacat tagcaaacct tgatgtttgc gggacgattc
    21481 aaacgttgac cttttatcga gaaaatttac taactgagga aaaaccaggt gtttgggtca
    21541 ataaacagct ctctcaaacc aattcgttat ccttaaaggt tgaagtaaac gggattgtct
    21601 accatttgtc agaatcggat catcgtattg aagtcgatgt gattcaagac tgtttgccta
    21661 gatatattca tcattattcg acgtttaagg tcattgtagt atcctttgct cccatactgg
    21721 ctaataagcg tttaagtatg cttgttcaac aggtttacat tgtgaatgaa acacacgaaa
    21781 gccttcaagt ttcagtgaaa gatgtgcctt cgtatcaaga gaagtattcg gatcagcaaa
    21841 atgtgttgat ttctcaaaaa ggaaataagg acacgatcgc acaacaggaa gtggcaagct
    21901 tcgccattgc ttttgtggac ccaaatgctt ttgaagaagt aatgactttt caggaatctg
    21961 atatcgaaaa atggctgaag gaaacattga actattttgc tcagctattt gggcagctgt
    22021 caatgccaaa cgatagagtt gtccatctct atcgtcgtgc actttatcaa agcttttcta
    22081 gtttcggtat gaatcgacag ggacaggtcg tcggcagcaa ttggggcagc tatcctgcta
    22141 ctaatcgaat ttggaataag gatatgtatt acgcatcatt gccattcacc atgtttgaac
    22201 cggagttatg tcaaaaaacg gttctatggt ttgatcaata tggcgttaag tttcctggtt
    22261 caaaatttac aggaggtatc aatcattctc tgagtaattc gttatccagt gcgttactgg
    22321 ctagtcttta ttttgaacat actggggata caaaattctt tgagcggtgt ccgcaggttc
    22381 tactgaatgc ttcaagaatt attgaggaca tattagctca acgtcggtca ggggaaccaa
    22441 tgctttttcc atctgtttgg ttatcagacg cctttgcact tggaaagtat cataccggca
    22501 gcaatctttg tctttggaag gcctgtattg gacttgcaga aatttttgaa gccctagaac
    22561 aactgagtct tagcaagcga tacaaaaaca ttgcctgtaa actaaaggaa tcaatttgta
    22621 aacaaatgac tatcgatggt ccttttggtg agcaattttt ggagggaatc ggtgatgagg
    22681 agaaaacaac ctattcagtg aagcactatc aaaaaccgat tcttgagcaa gggttaatct
    22741 ttttaagtga tgtgattgtg gatggaaaaa ttaatttatt gatgcatgat ggagaagaat
    22801 cagacactac gttgatgcct ttttatcaat ttttagataa taaagatcat ttatatcaga
    22861 acaccatggc cttttctgct agttctttca atccaaccta tagcgaggaa atcaaaggca
    22921 tcacttgggg attagaatcc ggcgccactt ttcctggctt tatcactgtt ttaatgagcg
    22981 agctgcacaa ccctcaagct ttcgtaaccc gattagaaga actaatttgt ttggcagatt
    23041 tagacggttc gtggtggtgg tggccgtatc gcttagaggc tcaacaagga gaagtcgtgc
    23101 gagactttgg ctgcggcaaa tgcggctggg catcgggtct ttttgtcagc ctaatgatca
    23161 gtcaatattt cggattgaaa ttcaaaaaag gagtattgca aattgatcct gttgataatt
    23221 taacgttttc ttggaagaat ttgaagttcg gtcaagcctt catttcgatt gaatgtacgc
    23281 aatccgagtt gagtatttca aatttgtcag aaaagccatt gaaaataagt gatacgaaaa
    23341 aaattcttca cgtagaaaag ggaaaaacga ttcacattca aaggagaaaa agatgagtat
    23401 actaaaaaat attacccaat ttaaaaaaat ccagagtcga gcaatcactc ccgaaaatcc
    23461 aacaggcgag aaaggtaaag ctgcgatgtt gccaagcgag ctaggaccaa gccgcaaagg
    23521 tcgtggcgca attccgctga cttctggtga agaaactgtg attgcggata ttactggaac
    23581 gggaataatt cgtcatatgt ggatgacgat tcgtgatcga acagaatttg gcagttttgt
    23641 tttacgtgat ttaatcttaa gaatttattg ggatgggtcg gatagccctg ctgttgaaag
    23701 tcctttaggg gatttctttt gtaatgggtt tggagagcgt tgcgaggtga actcgttgcc
    23761 gatagtggtt aatcctacag ggggctttaa ttcctatttc gaaatgcctt tccaaacatc
    23821 cgctaaaatt acgatcgaga atttacatcc gaaggatatg tcctcatttt tttatacgat
    23881 aaattacgct ttggtggacg aattggaaga aaatagccta tattttcacg caaattggca
    23941 tcgggaacgc attaccaaat tacaggagga ttataagtta attgatcgat taaagggtca
    24001 aggctattac gtgggtactt accttgcgct tacctgccta gaacgttatt ggtggggcga
    24061 aggcgagttt aaattctaca ttgatgggga tagtgagtat ccgaccgtaa cctcaacagg
    24121 ttcagaagat tattttggcg gggcgtgggc ctttcatgag tacgatgagt tcggacgtcc
    24181 acatgcgaaa aactattcaa cagccttttt agggtatcct taccaatcaa atagagatgc
    24241 cacaagagat cgttttgaaa cgggaaaatt gaatgccgtt catgcctttg gtgacgacgc
    24301 aatgccacga catggtcttt atcgttggca tcttcaagac cccatctgtt ttaccgaaga
    24361 tttatcggtc actttacaac aaatcggcaa cgatgatgtt cgcttatttg agcggcaaga
    24421 cgatgttgct agtgtcgctt actggtattc aacagaagct tcaggaaata ctaaacgtca
    24481 tttcactgat cgagaacgct tgccgcgtta atgaagtgtt tttcacatat tcgaagggag
    24541 gaacctatga tgatatatgg ggcaattgaa gctggaggga ctaaatttgt ctgtgcagtc
    24601 ggtactgagg aattaacgat tttggaaaga gtcagttttc ccacaacaac tccagttgaa
    24661 acaatgaagc aggtcattga tttttttaaa ccttttaagg aacaattggc aggcatcggg
    24721 gtcggctgct ttggtccaat tgatattcaa tgcaaatcaa cgagtttcgg tcatatcaca
    24781 tcaacaccaa aacttgcttg gcaaaatttc aattttgtag gaacgctaaa gcaatatttc
    24841 aatattccga ttgaatggac aacagatgtc aatgcggctt gctatggtga atatgttgct
    24901 ggttctggca aagggttagc aggtgttgca tacttcactg tgggaacagg tatcggaggt
    24961 ggtgccttgt tcaatgggac tttcgtggaa gggttcagtc atccggaaat ggggcatatg
    25021 ttgatcaagc gacatccaaa ggacaatttt tcaggaaact gtcccttcca ccaagattgt
    25081 ttagagggga tggcagcagg accagcgatt gaaaaacggt tgggtgtaaa aggccagaat
    25141 ttattggctg acgattcgtt ttggcagatt gaagcctttt accttgccca atgtgcatac
    25201 aatacaacac tgatgttttc gccagataga attatttttg gcggtggtgt aatgaaacaa
    25261 gaacacatga agaaaaaagt tcaggataaa tttgttgagc ttataaatgg atatgttgaa
    25321 ataccaccaa ttgatagcta cattattacg cccgaattag gtgataatgc cggaataatc
    25381 ggcggattag cattggccag aaaagcggta agaaataagc aaccataatt aatgaatgtt
    25441 tcattgatac tcatgtcaaa agtatttaac cgagtaatca aatgatcaaa tgaagttgga
    25501 taaagcgaaa atgaattcaa ataaaaaggc tctaagtcaa atcgtgttgt tttcattaaa
    25561 tcactgaaag gaaatgcttc tgcgtttcct tttttctttt tgttgtgcgc tgtatacctt
    25621 gaggatattc actggtggcg tacacttaaa tagtaatata ttgtgttact ttatgtctgt
    25681 ggtaatagtt atagttgctt accaatgtat gttcattttt cgaaatgttg acttcataat
    25741 ccttggctta atgtttgaat ttttttggtg atagattttt tcagccgtat aaaata
//
LOCUS       DADVXU010000033        24253 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.035, whole genome shotgun sequence.
ACCESSION   DADVXU010000033 DADVXU010000000
VERSION     DADVXU010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 24253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.035"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(44..1339)
                     /gene="purB"
                     /locus_tag="KB953_001269"
     CDS             complement(44..1339)
                     /gene="purB"
                     /locus_tag="KB953_001269"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBB6866257.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1352..2476)
                     /gene="purK"
                     /locus_tag="KB953_001270"
     CDS             complement(1352..2476)
                     /gene="purK"
                     /locus_tag="KB953_001270"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HBB6866258.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2469..2957)
                     /gene="purE"
                     /locus_tag="KB953_001271"
     CDS             complement(2469..2957)
                     /gene="purE"
                     /locus_tag="KB953_001271"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBB6866259.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3373..4680)
                     /locus_tag="KB953_001272"
     CDS             complement(3373..4680)
                     /locus_tag="KB953_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBB6866260.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4683..5264)
                     /locus_tag="KB953_001273"
     CDS             complement(4683..5264)
                     /locus_tag="KB953_001273"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBB6866261.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5380..5477)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5836..7128)
                     /locus_tag="KB953_001274"
     CDS             complement(5836..7128)
                     /locus_tag="KB953_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBB6866262.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7125..7748)
                     /locus_tag="KB953_001275"
     CDS             complement(7125..7748)
                     /locus_tag="KB953_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866263.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7751..8587)
                     /locus_tag="KB953_001276"
     CDS             complement(7751..8587)
                     /locus_tag="KB953_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBB6866264.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8604..9491)
                     /locus_tag="KB953_001277"
     CDS             complement(8604..9491)
                     /locus_tag="KB953_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBB6866265.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9507..10790)
                     /locus_tag="KB953_001278"
     CDS             complement(9507..10790)
                     /locus_tag="KB953_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBB6866266.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11473..12156)
                     /locus_tag="KB953_001279"
     CDS             complement(11473..12156)
                     /locus_tag="KB953_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBB6866267.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12174..12791)
                     /locus_tag="KB953_001280"
     CDS             complement(12174..12791)
                     /locus_tag="KB953_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBB6866268.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12801..13625)
                     /locus_tag="KB953_001281"
     CDS             complement(12801..13625)
                     /locus_tag="KB953_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBB6866269.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13618..14178)
                     /locus_tag="KB953_001282"
     CDS             complement(13618..14178)
                     /locus_tag="KB953_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866270.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14352..15308
                     /locus_tag="KB953_001283"
     CDS             14352..15308
                     /locus_tag="KB953_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBB6866271.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15613..16569)
                     /locus_tag="KB953_001284"
     CDS             complement(15613..16569)
                     /locus_tag="KB953_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HBB6866272.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16673..17275
                     /locus_tag="KB953_001285"
     CDS             16673..17275
                     /locus_tag="KB953_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBB6866273.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17363..18862)
                     /locus_tag="KB953_001286"
     CDS             complement(17363..18862)
                     /locus_tag="KB953_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HBB6866274.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18878..19402)
                     /locus_tag="KB953_001287"
     CDS             complement(18878..19402)
                     /locus_tag="KB953_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBB6866275.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19700..20449
                     /locus_tag="KB953_001288"
     CDS             19700..20449
                     /locus_tag="KB953_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBB6866276.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20440..21225
                     /locus_tag="KB953_001289"
     CDS             20440..21225
                     /locus_tag="KB953_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HBB6866277.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21194..21910
                     /locus_tag="KB953_001290"
     CDS             21194..21910
                     /locus_tag="KB953_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBB6866278.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22012..23046)
                     /locus_tag="KB953_001291"
     CDS             complement(22012..23046)
                     /locus_tag="KB953_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HBB6866279.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(23050..24108)
                     /locus_tag="KB953_001292"
     CDS             complement(23050..24108)
                     /locus_tag="KB953_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBB6866280.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
BASE COUNT         7187 a         5222 c         4224 g         7620 t
ORIGIN      
        1 ttttgctatt ctattttaat tgaattttgc aactcactct tctttattcc aaacctactc
       61 gtttgaatat cacgtcaacg tttttcaaat gatgatgata atcaaaggca tcgtctattt
      121 cttctttaga taataccgat gtgatggttg gatcttcttc taacagcggg cggaactgca
      181 cttggttgtc ccaagcgtac gcagtttttg gctgaacaag atcataagct tcttctcttg
      241 tcattccctt atcgatcaat ttcagcataa cgcgctggct ataaatcaaa ccaaatgttg
      301 cagacatatt ccgcttcatg ttttcaggaa agaccgttag atttttaacg atattgccaa
      361 aacgatttaa catgtaattc aataaaatcg tggtatcagg caagatgatt cgttctgctg
      421 aagaatgaga aatatctctt tcatgccata atgtgacatt ttcataggca gtgaccatgt
      481 gtccgcgcac cactctggct agaccagcca tattttctga accgatcgga ttccttttgt
      541 gcggcatagc agaagaacct ttttgacctt tcgcaaaaaa ttcttctact tcacgtgttt
      601 cggatttttg caatccgcgt atctctgttg caaaacgttc aatacttgta gcaataagcg
      661 ccatcgctga gaaatattca gcatgtagat cccgagggag aacttgagta gagatttctt
      721 gtggacggat ccccaattgt ttacatacat actcttcaat aaatggcggg atatttgcga
      781 acgttcccac tgctcctgag atttttcctg cttctactcc ttttgccgca tgttcaaatc
      841 gttcgatgtt ccgtttcatt tctgaatacc atgtggcaag tttcaatcca aaagtagttg
      901 gttcagcatg gacaccgtgt gtccgaccca tcatcactgt atatttgtgt tcttttgctt
      961 tttcgccaat gatttccgta aaacgtttca agtcttcccg taaaatatca tttgcttgct
     1021 tgatgagata gccataagca gtatctacga catccgtact tgttagacca taatggaccc
     1081 attttctttc ttctcccaat gtttcagaaa ccgctcgtgt aaacgcaacg acatcatggc
     1141 gtgttaattt ttcgatttcc aagatccgat cgatatcaaa tgatgcatgc tcacgaattt
     1201 ttttcacatc ttcttgcggg atttcaccaa gttctgccca agcttcatct gctaaaatct
     1261 cgacttcgag ccacgcacgg tatttgttct cttccgtcca gatattcccc atttcagggc
     1321 gtgtataacg ttctaacatg ttttttctcc tctaatccca aatattcgtt tgtttgattt
     1381 cttccagcgt ttgctggatg tcttctgtca agatagtgat atgtcccatc ttgcgacctt
     1441 tttttgcttg tttttttcca tagaaatgga attgccagtc tggcttattc tcaatcaagc
     1501 gatagctttc cagcagttgt tctcccaaaa cattgaccat tactgcattg cttatcaaac
     1561 gtactttttc tcctaatggc caaccacaaa tacctcgaat atgggcatcg aactgactca
     1621 tagaacaagc ttcaatagaa taatgtccag aattgtgagg acgaggagct aattcattca
     1681 catatagccc gcctgttttt gttaaaaaca tttctacccc taaaacacct gaaaggctca
     1741 ctgcttctgc aatcacacgg gcaatacgct cagcctcctc gatcacatcc tgatcaatcg
     1801 cggctggagc aatcgtttca tgaagaatat tgttgtggtg gatgttttcc acgaccggaa
     1861 aagtcgtgaa atctccttga ccatttcctg ccaccatgat cgaaatctct ttttcaaacg
     1921 ggatccatgc ttctaaaaca caggttcctt ctcttaaaag gtccatggca ggagctagat
     1981 ccgcccgact tttcaaaaca tactgcccct tgccatcata gccgcctcga gtggttttca
     2041 aaacacaagg atagccgatc ccgtcaatcg catcttggat atccgtcgga ctgacgatcg
     2101 ttgcataagg agcaatcacg atgttattcg tttccaaaaa agatttttct aacaggcggt
     2161 cttgcgtaat cgcaagcaag tctgtccctt gaggaataaa tgacatgggt aaaatcgcat
     2221 ttaacgcctc tacactgacg ttttcgaatt cataggtaat gacatctgtt cttctggcaa
     2281 gctctttcag cgcaaaggtg tcatcataga cactttcaat gtgccaatct gcaacttgtg
     2341 cggctggaca atcgctcaca ggatccagca cacctacttt aaagcccatt tcttttgcac
     2401 taatcgccat catacgacct aattgaccgc caccgacgat cccgatcgtt gaaccaggca
     2461 acaaaggttt aaccaagttg atcactactt tctataacag ttttagccaa cattgaacgt
     2521 ctttcagcca atttagcctc caattctaaa tcatacatgg aaagcatctg tatcgcaagt
     2581 agtccagcat taattgctcc cgcttttccg attgctgtag tagctacagg tactcctcca
     2641 ggcatttgga caatcgaaag aagcgaatcc aaaccattta atgttcgtga ttggaccggt
     2701 acaccgataa ccggcaaagt tgtttttgat gcgaccatac ccggcagatg cgcggctccc
     2761 cctgctcctg caataatgat tttgattcct ttttttcgag cgttttgtgc atattcaaac
     2821 atcaaatctg gggttcgatg agcagaaacg accttttttt cataagacac accgaattca
     2881 tccaatattt gacatgcttc tttcattgtt tcccaatctg aagtactccc cataatcaca
     2941 gagatttttg ctgtcattgt ttttcctccc agtttttcta cattcatatt ttaacaatca
     3001 ctttttttga tgtcaaagaa atttcgaatg ttaacaaaga aaaacatttt attgttcgtg
     3061 tttattaaag ataaaaaaaa ggaatgtgac aaaagttagg tcacattcct ttttatggat
     3121 aaacggtgtt ctcctgccaa ctcctcggtc acaaggaaca atattcaaca aacatgagaa
     3181 gctttttttt gaaattgttc cttgtgactc ggagatgaac ggcaggctca cgacctcttc
     3241 cttcaacggt gttcaatcag ctgaccctcg gtattagctc aaacaaacgg caaaatacga
     3301 ggaactattt ttccgtttgt ttctctaaca atcaggtcaa aacgctgatt tcacaacctt
     3361 tcttttgctc tattacttct tgaaggcttc ttccaatatt tcttcatctt ttttcaatcc
     3421 attaaagatc aaattcaata tgatagccgt aatgctactc attacgatgc catttccagt
     3481 gaacatttgc aatgtttgcg gcatttgact gaataatgtc ggcgtaacat tgaaacccag
     3541 cccaaatcca acagaaacag caacaatcaa tagattttta tcattgctga aatcaacttc
     3601 caacagcata cggattcctt ggactgccac catcccgaac atgactaaca ttcctccacc
     3661 aagtaccggc tcagggatga tttgtgctaa cgcaccgatt ttagggaata atcctaaaac
     3721 aatcaagaag aatgcagaga aatagatggg ttttcttgtt ttgattcccg aaagttgaac
     3781 caatccgaca ttttgagaaa agccagtata agggaatgta ttgaaaatac cgcctaagat
     3841 aacggcaagt ccttctgcac gatatccctt tttcaattct tcttctccaa catttttccc
     3901 agtaatgtca ccaagtgcaa aatatacacc tgttgattct accatgctga ccatggaaat
     3961 gatgatcatt agtaaaatcg accatacgtc gaaagttggt ttaccaaaat aaaagggttg
     4021 tggaacatgg aacaacggcg catctccaat cgcgctcaga tcaatcattc ccatgaaagc
     4081 agctaagatg cttcctccga ctaaaccaat caataccgcg attgaacgaa tgaacccttt
     4141 tgccagcatt tgcacgccta caattaggag aatcgtcaca aatgcaagta gcagatttgt
     4201 tttgtcacca aaatcagctg ctgttgcatt acctccaccc attttctcga ttgctacagg
     4261 aatcaaagtc aaaccaataa cggtgataac cgttcctgtt actataggag ggaatagttt
     4321 tttaatttta gaaaagacac cagaaactaa cacaacaaag atcccagaag cgataataga
     4381 tccgtagata gctcctactc cttcattggt tccgatcaag atcagtggag ctactgcttg
     4441 gatcgcacat cctaaaacga caggcaagcc aattccgaaa aatcggttaa ctgtcaattg
     4501 caataatgtc gctaatccac acataaagat atcaattgaa atcaaataag tcatctgttc
     4561 ttgattaaag cctagtcctg taccgatcaa aagaggcacc gctactgctc ctgcatacat
     4621 cgctagcaga tgctgcagcc ctaaaaccgc agcttttcca ttttgtgttt ctttttccac
     4681 gattatgcgt cctcctcaag aaactcaact tgtccattcg tcaaggaagc aatacgcgca
     4741 agagaaacga ctctcaatcc catttcttca agtaactgac gtccatcttg gaaagacttt
     4801 tcgatgacga tcccgattcc ttctacttgc gctccagctt gctggcataa ttcgatcaat
     4861 ccttttgccg cttgtccgtt tgccaagaaa tcgtcgataa tcaatacttt gtcttctgaa
     4921 gataaaaatt tacgagaaat agaaatcgta cttgtcactt gtttcgtaaa agaataaaca
     4981 gaagctgtca ataattcttc atccatcgtc aaactttttg cttttctagc gaagatcatt
     5041 ggaacatcta attctcttgc agcaaataaa gctggtgcga tcccagaagc ttcaatggtc
     5101 acaactttcg tgattccttg ttctttaaat acttctgcaa accgttttcc catttgttcc
     5161 atcaatacag ggtctacttg gtgagtgacg aagctgtcga cttttaatac gccctcgcct
     5221 aatatacgtc cgtctttttg aatacgttca actaattctt tcactttatt tgctccttta
     5281 tcatttatta ataccgaaat aaccgctttt gtgcaaaaac ctactatgtt tctgttaaag
     5341 aaaaacaccc tccactttat cataagaaaa gaagggtgaa atagccaatc ttttacttat
     5401 agtccagcat ttacggtact ggctagaaac tgtcatccat attatgacga tatataagta
     5461 ggtatgtaaa actaataata gtgtaaattc attttcttgc aactgttttg cacgtgaagg
     5521 cagtttgcca tatttgaata aaaaatgttg tttcaaatat tgaaaacagt ctaacactac
     5581 tttctgaaac tgacaacgtg gtttacttat ttttttgtaa aatctaatga tttttaatgc
     5641 attattcctt tttgttcggg tttaaatagt tatttttatt ttctcctatc taaaaaagca
     5701 gcccacataa aaagaaggca tgacaaaaag catgtcatag cccctttttc cgaataaata
     5761 gtgttcaaaa gacagcctat cggcaataag gcaagatttt ataaactacg ggaagtaatt
     5821 tccgtaaatc tattcttatt cttttaagct gacaagctcg tcaatcacta ctgcttttcc
     5881 tgaagcaata gaatgatttg ctgcgattcc tgtcaaaatc gacaaaatac catctacgtg
     5941 agttgctgcc cgattccacg gatcttcttc tttttcttca ccaaatagat catctaataa
     6001 acgcggatct cctccgccat gtccgcccgc ttcttgttcg acgattacct catatggctc
     6061 accgaacatc ggatagacat ggatggactg aagaacggct gctccttcat cctttttatc
     6121 tccacctgca ttgatgtaag acttttcgat cactttcatt tcgatccgtc ctttgctgcc
     6181 attgaaagaa acattgaatc cttcccacgg catatacgcg tttagcgaat acgtcaaaat
     6241 cgcaccagtt tcatatttga ccgttacgcc tagtgtatct tcaatagaga tatcatcgga
     6301 aaatacactg cggtcacgaa tatatccact gtctttttct gcttctaaat acatttctcg
     6361 caattcctca ttggctgaca ggtctatagc aaatggatca gctttagcta gttcgcttcc
     6421 atagcatcga ctgtaaaata tttctactcc gcgtttctcc gcattatgca tcccataaaa
     6481 gtttaatcca ccgaatgcaa ataccttttg gggacggctg ttcaaccaaa aattcactag
     6541 atcaaaatga tgggtagatt tatgaacaag aagcccgccg ctattgtgct tatttcgatg
     6601 ccatcttctg aaataatctg ccccgtgttg cgtatttaac agccattcga agtggataga
     6661 aaacacatca ccaatcacat cactttctat cagttccttt atcttcgtgt gatacggtgc
     6721 ataacgataa ttaaaagtga ctcgcaggct tcttcccgtc tgtttttgga ccgttattat
     6781 ttcttgagct ttttcctcat cgatagtcag aggtttttcg caaataacat catatccttt
     6841 tttcattgca cgaatgatat aatcatgatg cgttcgatct actgacgtaa taatgacttc
     6901 agccggccgt acgtcatcca gtagttgctc aaattgtgat ggcatacatg tctttacttt
     6961 tttctgtcca tacttgttca caagtatccg atttgcataa tccatccttg tttgagatac
     7021 atcacaaaaa ccgactagtt cacaagtttg atgatacgta gaggcaattg cctcataaaa
     7081 aaaacgcgct ctcccaccaa gtccgacttg tacatatttt ttcttcattt aaagtcattc
     7141 tccttttctt ttaagcaaaa tactgtgaca aatggcagcc ctggatacag taatgcagtg
     7201 agtaaaaaat catgctgtat acaccagaac agtccataca aaaggcataa ataccacaga
     7261 aaccccttga cgttttttga caacagccag atattccttt tgatcaaata aacaagacta
     7321 tctgctttat cttctttgca gatatcaatt agcgtactaa aaaaactatg ggatacaaaa
     7381 agcaacagac acaatagaaa aggaatcaga taccaagctc caatctttcc cactaataaa
     7441 taccaatcaa ctgcaaaaat aatgacaaac agctgaaaca acgtaatgat gaaacacatc
     7501 cgtttgattt ttcctttcct ccaacatacc tttttttctg ccaagtaatg gacagataga
     7561 taataagaag gatacatcat caaaaaataa atcagataga caacacgagc agacagcact
     7621 aatcctaaag tgaacaaaca tcctagaact aaagcgaaaa gagcattact gataagactg
     7681 gttaaaaaaa tccgacagac atagtagttg agcttgtaaa gaaacatctc ttctattggc
     7741 tttttcatat tcagcccttc agaccgcttg tactgatccc ctggacaaga tatttattga
     7801 agaataagaa aatcagaaaa accggtagca gcgacaacgt agacatcgca aacatactcg
     7861 cgtaagtcgt actagatcca gcgtctgcaa ataatttgat ggcaatcgat accgtgtaag
     7921 atctcggctt gtttaaataa atcaatggtc ccatgaagtc gtcccatttc caataaaact
     7981 ggatgatgat cgtcgtgatg atcgaaggtt tcagcaaagg aaaaataatt ttggtaaaga
     8041 cactatactt gctgcatccg tcaataatcg ctgattcatc taactctttg gggatgctga
     8101 ccatgaattg catgatctgg taaatgaaaa atgcttgtcc aaaaaaatgt ggtaaaatca
     8161 gcggcacata cgtcccaacc aaattcagcc gattgtaaat aataaactgc ggaatcaaaa
     8221 tcacttgtgc aggaatcatc atcgttacta acatgacgac gaaccagaat ctttttcctt
     8281 tgaaatcgat tctcgataat gcgtaagcaa tacaagtcga gctcagaact gtccctatcg
     8341 tcgaaactgc agagatgaca aacgaattcc taaagaaagt aaaaaatgaa attccagcaa
     8401 accccgccca tccttttgcg taattttccc attgaaaagt ctctggaatc aaattcagcg
     8461 aaccactcat aatctcttga tccgttttaa aagaacctgc gatcatccat aagaccggat
     8521 aaatcatgac tagacctaat agcaaaataa aaccgtattg aaaccatttc gttactgtat
     8581 tattcatttc ttcctcctct cattcacaca acgacccatt tttttgatga tttgaaaatc
     8641 agaaaggtga cagctcccat aatcaacagc atgatccacg aaatagcact agcatacccc
     8701 atagaattaa aagaaaaagc ttggttatac acataaagag cataaaagtt cgtggcatca
     8761 ttcggaccac cttgggtaat gataaatgcc tgcgtaaaag tcataaacgc cgcgattgtc
     8821 tgcataacca agttatacaa aatgatcggc gaaagaagag gtaacgtgat cttgtaaaac
     8881 gtctgccatt tgttcgctcc atcgatctct gccgcttcat aatacgagcc tgggatctct
     8941 ttgatacctg cagcaaagat gatcattgcc gaaccgaatt gccaagcaga catcaaaaca
     9001 agagggatca tgaccagttt ttgatcgcta aaccaggaaa gcgcaggtgc gcctaatcgt
     9061 tccaggaaaa tattcactaa cccgttttta gaaaacaact cccgccaaac tagagcgata
     9121 gaaatactgc caccgatcaa actaggcaca taatacaaag aacgaaagaa agtcgttcct
     9181 ttgatattcc tcgttaaaag ataagcaatc aatagaccaa agatcagttt cacaggtaca
     9241 gatgcaaata catagagaaa agtgactcgg atggagttca aaaaacgggc atcactcagc
     9301 atctttatat aattatccaa gccgatccat gagactggat ttcccaaacg ataatcggtg
     9361 aacgaataat aaagtgacaa gatcatcggt atcactgtta tcaatagaaa tcctaggatg
     9421 aatggagaag caaatacgta accgaccaca ttgtcttgat ccaagaaccg ttttagtttt
     9481 tcttttttca tgccttcctc ctgctgctat cctagaatgt ttgacgcaaa ctcataaaga
     9541 ctctctgctg cttcttctgc tgttacttct tcataaatga ctttttcaac taaaagattg
     9601 tattggtctt gaatttccgg attcaaagca ctatcaaaga cattcacttc gccgttgtcc
     9661 atatcggaaa tcatttctaa ataatcaaat gtcgcttgca aagttgcatc atctgcacga
     9721 gcgagctgtt ctcgaatatt cgacatgatt ggtacaccac gttctccttt taaaatttca
     9781 ttggcttctt cactgttttg aaagaaatca ataaacttag cagcttcttc tttatgggct
     9841 gagctttttg ccatactcag catttgcgaa gaacgtaaag ggataccaga ttctccatct
     9901 gcttttctgc gaggtggatt aatcaactga atctccatat cttcaggagc ccctgacaat
     9961 atttctgtca actggttact tgatagaaac accattgccg cttctctatc cctaaccgcc
    10021 tgaaaagaca gatccgtcgt acttgcagct ccaccacctg gttctggatg ggcccctttg
    10081 tctaccagat ttttacgcag ctggaaaaaa tctgttagat agtctggatt ttcaaatccc
    10141 aagctgttag atagatcttc ccgattataa aaattcaatc ctttctcaac ttgtggtatt
    10201 tgaataatac agccagctat aaaatcttcg aatcttgaaa tagcatagat tcctaattct
    10261 tcagtgattt gatagctgat ttgttcaaat tcatcccatg tccaatttga agctggttca
    10321 gggatattta attgttcaaa tatattttta ttgtaagcaa tcgcaaacga attcaccccg
    10381 ttagacagtc caactaattg cccgtcgtaa gtcgtcgttg ctaaatagga ttcatctgca
    10441 ttagaaatat caattatccc ttgctccaca tattcatcta atggttcaat ctgattgatg
    10501 tattgaggaa attgattgtt cagttggaaa atgtcccaga tatcatctga tgctgctagt
    10561 gtacctaatt ttgtgtaata tccttcataa tcataaaatt cataatcgat cgtcacatgg
    10621 ggattttttt cttcataaag ctcgattact tttactgttg catcgtgtcg gacttgcgaa
    10681 ccccaccaag ccatgttcag cgtgattttt ttgtcatctt cttgcccaca agccgtcaag
    10741 aagaataaca taacacttaa agctaagatg attctcattc ttttcttcat tctctgcacc
    10801 tctacttcca ttgttatttt tcaatatact gtatttttat acagaacata tttattaaac
    10861 gatctttttc aaggggaata aaaaccaaga cttgccttat cgttctgcct aggatgaaca
    10921 actcaatact ttcaagaaaa attagatagt gctgattctt ctttcatcgc taaaaacact
    10981 tcttgcttca atgatgggag gataattacg caaagaaaaa aggatatcag acaactgccc
    11041 aatatccttg acatagctaa ataagcatgc ttcttcgtgt gcgctcttcc ctaaaaaaca
    11101 gaaaaataac tagtaattct gtatgaaatt aggtacaata tacacgtcga tgcacaagca
    11161 ttgactatgg caacttgatt ggtcagttcc cgctgacttt tcaagccccg acaatttagc
    11221 tgctactaaa ttgtcgggtg ccttttttag tttttattcg tttttcattc tatagaaagc
    11281 gcttcaataa atcaagacat ttttttgtta gaatgctaat aataacaaaa ggttttaact
    11341 cttcttatgt acattacagg taaaaaaaca aaaaaatttc tctttttttt gaattctgaa
    11401 caaatagtac tggagtacaa aagacgagga agagccacta tctgctcttc ctcgtctttt
    11461 gattctcacc tgttattcat gattcttttt ctcagtttcc cgatattttt ccattttacg
    11521 actaaaaagc tctccgctac ttggtggcgt atatgtgatg gcatttgctc ctgcttgaat
    11581 ggtctcagca atactttctt ccgttggacc acctgtcgca atgataggaa gttcaggata
    11641 ttttttacga aaatggcgta ctgcttctgc agtttcttta cctgcactga tattgataat
    11701 cgaaatacca gctgctaact tttcatctat ttctgtatat ttcgacgtta ccgtactgat
    11761 aactggaata tcaactactc gacaaacttc ttttactgtc tctaaaggcg taggaccgtt
    11821 caaaacgacc cctaacgaac cttgcgcttc tgcgaacatg ctcatgtagg aagaacgagc
    11881 accttgtgtc aaaccaccac ctacaccgga gaaaactgga atgtcagctg cctcaataat
    11941 gcttttagta atcgctggat gaggtgtaaa aggatacaca gcaattactg cattcgcatt
    12001 tgtattacgt atgatcgcaa tatccgtagt aaagatgatc gaacggatca attttccaaa
    12061 tatcttgatc ccgcttgctt ctaaaatgat ttccggtact cttacgatat cttttctcaa
    12121 ttccgtcatg atttctggcg taaattgttc tgccatcagc cacttctcct ttgctatctc
    12181 cggtcatact gattcaattg atacgtctcg atgacctgga tgattttttg cgcataaccg
    12241 gggtctgtcg cataacctgc atcttgcaat gcttgtgcgg cttcctgata gttggtggcc
    12301 gtaatcactt tttcatacag cgcaggattc caatctactc cttggacgaa cagttgtgta
    12361 tggtcatcca tggactgttc ccatgaatca tacactttga agtctccttg gatagttatc
    12421 cattcttcgt taacaaattc ctttgtttcc aggcttactt tcttctgatc cccataagcc
    12481 ttgatcccga aaagattatt gtatttgcta gccaaggtac tttttcccca atttgattct
    12541 aatatagctt gtcctaaaat gatactagga agcactccat aaccatcttg tagttcttgg
    12601 gcatgcggaa caagccgttc aataaattgc tgatgggatt gcgcttcatt ttcttgctga
    12661 ttatcagtaa aaggagaaga cagattcttc aaggaaaaaa caaaggctaa gagtatcact
    12721 gctacaccgg caaccactgc tggccaacgg atattcttgc gcttttgctt tttccacttt
    12781 tttttcgcca tgatcaattc ctacttttca tttaattcat tcagatattc ttctagcgtc
    12841 aattggtgtt tggtgatata ctcagcgttt tctttaccga cataacgtaa gtgccaaggt
    12901 tcatatgtga tcttagttat atcttcgtgt ccttttggat aacgaacgat aaatccatac
    12961 tgcgcagcat tatctgcgat ccatttagct ccaggctgat ctccgtaact tgcatccaat
    13021 acagtagaag gataactgtt gtaccagttt tgatcgacga catcgactgc cagacctgtg
    13081 tggtgttcgc tgtatcctgg ctccgtaatc gtttctttag taatctttgt cgcctcctct
    13141 tctgatacac cttgacgact catcacatct tggacatttt gttcgaaaac ttgttgttgg
    13201 gatgatacag accggtaagc tgagaccatc actaatggaa atccagctgc ttcagcagcc
    13261 ttggcaaatg cttcatattc tggagcaatg cgtttgtcga tcaagtatcc gttacttaat
    13321 gatgccaatt gagtagactc atcaatttct ttttctaatt tgtgatctgg accaaccaaa
    13381 actagcttcc aatcgtctgt tttaacatct ggcaattgtt tggacgtacg atcttttgtt
    13441 tctttgtcct ttaccttgct gctggttgtt tgactgttct tttctttact agcagttgtt
    13501 ttggtgcttg ttcgttcgac tgcttgattg ccggcttgaa catgcgaatc atcatcgcta
    13561 aaaataacat agcccattgc tgcgatcata atcacaactg ctccaaatcc taaaaattta
    13621 ttcacttgaa aaatcctttc gttgttcttc acgttcccca ttcgcctcat gaacgtaagt
    13681 ttcttccaga ttttcattca cccaatcgag aagttcgatt ttttctcctt cgatctgttt
    13741 ttgaacatct gctggcagac gaaaattcaa aatatcatca tagccccatt catcatattg
    13801 aatacttact ttcaagtcaa ggtatctacc agcctgctga ttatttttca aagggatcag
    13861 ctcgactttt gctgctttgc tgctaagcca tttttgcgcg atgcgtgttt tgatttgctt
    13921 gtactctgca acggcaacgt ttcgtttttc gggaaaaata atcgtttgac gcaaaatctt
    13981 ttgcgttagc atccattcat aatcagtgaa aagatttttt actttctgca tcgcttcaga
    14041 tggcagattt ttctcgccat ctttccattt ttgccattgt tctggactga tcagcaagta
    14101 ttcatctaaa aaattttttt cagtttcaaa ctgttctaat aatacactca taataagatc
    14161 aatatatact tgatgcacgt ttatcccctc cttttaacta ttctacttaa gttttgatca
    14221 aaaagcacct cttaaatcgc tgctttcatt ttttttaatg tttcaagtcc tctcctcttt
    14281 attcttcgtg atttttgtta taataataga cagacaagaa actcaatcca actatgaagg
    14341 agcgttttca gatgaaaaaa tatcaatggg gaatcgttgg tcttggtacc atcgcacatg
    14401 agtttgccga aagcttcaat caagagacga gcgaactaac agcagttgct tccagaactt
    14461 cagaaaaagc ggaaaacttc gctcaccgtt acaatattcc aaaagcttac ggaagctatc
    14521 aagaaatgtt agacgatgct gaaatcgaca tcgtgtacat tgctgtacca aatagacagc
    14581 atatcgacca tatcttggca gcattggaag caggcaaaca cgttctatgt gaaaaagcca
    14641 tcacgatgaa taaaaaagaa ctggctgacg ctatgcgctt agcagaagaa aagaatttga
    14701 tcttagcaga agcgatgaca atcttcaaca tgccacttta tcagcagtta cgctcaataa
    14761 tggatacagg aaaactgggg gctttaaaaa tgatccaagc acctttcggc agttacaaag
    14821 aaccagaccc taaaaaccgg ttcttcaatc cagaattagc tggaggagct ctgttggata
    14881 tcggtacgta tgctgtatct ttcgctcgtt tctttttgag ctctcaacct gaagtagttg
    14941 cctctacaat ggttcctttt gaaacaggcg tggacgaaca atccgtcact atcttgcgta
    15001 acaaagaaaa tgaacttgct gctgtcagct tgacttttca ggcaaaaatg ccaaaagtcg
    15061 gtatcgttgc ctttgagaac ggttatgtga cgattgctga ttacccgcgt gctgatcgtg
    15121 cggagatctt atttacagat ggtacaaagg aattcatcga aagcgggaat acatctgaag
    15181 caatgaatta cgaaataaag aatatggtac acatgattga aggtgacttg cccaatcgct
    15241 ctctcttctt gacaaaagat gtcatcgaaa tcttggatca aatgcaagtt ctatggaaaa
    15301 atatgtaatt ttaaaaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    15361 acaaaaatag ctcctcctat ttttcgtttg gctggactta ttaccgcaga ggaaagcttt
    15421 tgaacaccgt ttaagaaaga ggttgtgagc ctaccgttca gctccgagtc acaaggagca
    15481 atttcaaaaa acagcttctc atgtttgttg aatattgtga cttgtgaccg aggagttgac
    15541 aggagaacac cgtttaaaga agaggttgtg aaatcagctt tttgacctga atattagctg
    15601 aattcacaac ctctattttt ggattttttg ttgggagaat tctgccttga tttcaataaa
    15661 acttttaaaa gactgcaaaa actggaacaa ttctgctcga tcctcgaatt cagggcgact
    15721 agtcggcaga ggcatttgcc gatacaatac ataaacttct tcaatacgtg ttaaaatttg
    15781 tctgccatca tttgactctg aaaatgtttc tgcagtataa atcaataaac catagatatg
    15841 ttttccataa taagctggtt gctggattct ctctagattt tccagcatgt ctttaatcac
    15901 attggcctgt gctcttctca tagagaagta agtttcgtag tatagcggct gtctgagcca
    15961 gtagttttct tgatgttctc tggcatcttc ttggctttcg cgaatgaatg tcagtaaatg
    16021 ttcgcatttg atctgcacat cttctttatt ttctgataaa agaaattctg ccatttgtct
    16081 caaaatattt cgaaattctt tttcgatctg tatttggttt tccttcagct gcttcgtacg
    16141 atctggcatg taaacatttg caagcaaagc aaatactaat ccaatcgcca ttaatcccgc
    16201 ttcattgccg atcagctgcc aagacatgtt ttcctccact aaataatgag tgaccaaaac
    16261 cgaatttact actatccctt cggttaattt gaagcgagag gcaagcggga tgaacaggaa
    16321 taaaaaaaga ctgaagccaa aaatagtata cccaacagca aaaaaactga acagtgctgt
    16381 tgccgtagct aatataaagg ctgcgattcg attcatgccc gtacgcaatg tagagcgctt
    16441 ggtatttcct acacttaata atgcaatgat ccctgcagct ggccaataca acagcgataa
    16501 gctacccgct gtgaccatcg aaaagaagac actgatcact gttttgactg ttcgcattcc
    16561 tatacgcatt tacttccccc ttttttattt taattatact tgaataatga aaaactagct
    16621 atcagacaaa agtttaggta tgatagtaaa aagaccaaaa aggagaatct ctatgcaatt
    16681 tggccgtttt cgactaggta tgcgtacgat caaaagtgcc ttagctgttt ttttatgtat
    16741 cttgtttttt catttgactc aacgaggaat cccgatgatt gctgcattat ctgctgtttt
    16801 ttctttgcgt caggacttga ctacaaccgt ttcttttgga aagtctagga tcatcggcaa
    16861 tacattaggt ggtacattag ctatcattta tttttttgtc aaaagctatt ttcacaatga
    16921 ttttttggta gaattgatct tgcttcctat tctagttatt attgtgatcg tcgtgtcaga
    16981 tggtattaac aataattccg gtatcatttc cgcaatagct acgttgttgc tgattgcgtt
    17041 gagcatccct caaggcgaat ccgctttgta tgctatccaa cgtgtactag atacttttat
    17101 agggacattc attgcaatcg gtatcaattt ctttcttcgt cctcctgaaa cagaaaagaa
    17161 ggaagaacta tcagaagatt tggtcgaact ccaaaagaaa gaacgattgc taagaacaaa
    17221 tttacaagag atagaagcta agatccaaaa acaaaagaaa aatagcgagg actaattttc
    17281 ttttgcaata aaataaaaaa atggagagaa gaactttaaa aagtcttcct ccatttttga
    17341 ttgaacggtg aagtgtacta ctttattctt ccttcatcga gttttgttct gcctcttttg
    17401 gcgcttcata catatttact tggctagtag tcccacgtgt cgacaaattc tctacgattt
    17461 ccttcaagtc aagtcctgtt gtcgctttta gtgtttcttg agtagaagaa agcaaatttg
    17521 tggcataatt tgtcactcgg ttcgctccac tggtttctcc tgaactgctt cctgtatcga
    17581 caacagagat tttttcgata tttcctagcg gctgtgctgc ttcgcgcatc aattgcggta
    17641 gcatatcgat aaccatactc aagacagctg cttcgccata ttccttgaag gcattggcga
    17701 tcttttgttt tgcttcggct tctgcttttc cttttgctag tgctgcttcg gcttctgctt
    17761 gtccagctaa acgtgtctta ttggcttccg cttctgccag tgcttccaca cggaatcgtt
    17821 ctgcttcggc ttcagtcact tctttgactt tttgagcttg tgcttcttgt tctttagcgt
    17881 accgatcggc atctgctttt ttcttcactt ccgaatcata ttgcttttca cgacgtgtga
    17941 tttctttttc ttccagttcg atttgttttt gacgttctac gattttaatc tgcatttcct
    18001 gttcgattac ttgttgttgt gctcgggcac tttctaaatt gtaggcttgg tctgcttttg
    18061 ctttagcaac atcttgttct tgtttgtagg ttgctaattt cagttctttt tcttttaatg
    18121 attctgcgat ctctgtttgt cgctgtaatt cagctgcttg tgattctttt tctgcttgag
    18181 cttttttgat acgcgtttct tttagagctt ccgcttctgc tatatctgca tctcttttga
    18241 cttgggcgat tcttggtttc cctaaagagt caagataacc gtttttgtct ctgacctctt
    18301 tgatagtgaa agaaacgatg atcaacccca tttttgcaag gtctacagaa gcgacttctt
    18361 gtacgctttg actgaactta tcgcgatttt gatagatttc ttctacagtc atagaaccaa
    18421 gaatcgagcg caagtgtcct tctaatactt ctcttgcttc attttccagt tcttctcttg
    18481 tcttgcctaa aaactgttct gctgctgtag caatctcttc tacagatgaa ccgattttga
    18541 tgatcgaagt accatcacac atcaccggta cgccttgttc cgtatacact tcaggggtgg
    18601 agacgtccaa tttactcgat aatagactga tccgattcga acgttgaaaa actggcaaga
    18661 cgaatgcgcc acctccacgc acaattttga ttttgttatt gctttcatca atgtgcacat
    18721 ttttgcttcc taaataactt ccactgatga tcaacgcttc atccggtttt gctgtctggt
    18781 acttcgctac aaagacgatc agcaacatca gtaaaatcaa agctgcgatt gcgatcgcga
    18841 taaaaacaac tggtatttcc atttaacaca cgctccttta agcttttatc atgctttcat
    18901 aaggcatgac ataacagatt ctttccttga tctcaatgat caatactttc gttccccgct
    18961 cagctgtctg ttcttggggg ctgtaaaaac aagctggacg actaatggaa ccggtaacac
    19021 ttttcaactg aatttctccc atacctcgta cagggatcgg tgtgatcaca acagctactt
    19081 gtccttccag agttttttca gaagacgaca tcgttgattc ggcatgtttc atcggcatca
    19141 atatatagaa attaagcaaa aataccaaca ctatcgatat gcctccacta gcaataaata
    19201 aaaataaaga tgattgatct gttaaacgct cacctagaaa accaaataag cttgtgaaag
    19261 ttaaccaagg gataataagc atcggatcaa gcgggccatc aaaatcgaat acatctccaa
    19321 aaataatcaa taggactgcc aatcctgcac aaatcagcaa tgtataccaa taaatcgtct
    19381 caagtgcgat tccccctatc atatacacgc ctccttccat ttctacgtct atcataccaa
    19441 tgtttgaaaa ttctgtatag cttcttttac cgactcagtg gtgtttttta taggaaggtt
    19501 gtgtttatcg ttgacaattt gttaaatttt tgttttcatg ttaaaatttt cacaataaat
    19561 tctttcgagg tagcctatgt ttcctttttt taagaaaaaa ccattaaagc tccagaacac
    19621 gatcatcctt gtcgtttgtt tctccacatt cgtttctttg attgtttctg ccatattgat
    19681 caggaatttt attctttcga tgaaccgatc tagtgtgtca gataaaatct ctagcattgc
    19741 taaaatcgtt gccaatgata aactcatcca aaaaaagctg cagactggtg acccgacaca
    19801 tgcgattcaa ggaataacaa atgaaatcat gattgcatcg aatttagatt ttatcgttgt
    19861 catggaccct gaaggcattc gatattctca cgcagaccca agttacatcg gtcttccttt
    19921 ctcaagtcat gaagatgtga aaaaagtgat tggaggacaa gaatttttct ctgaaaaaga
    19981 aggaatgtta gggttcggct tccgctattt cgtccctatt tattatgatc aagagattat
    20041 tggtgcagtt tgtgttggct taacaaacaa cacgattgac aatcaagtca aagatggaca
    20101 aaaaatgatt gttttttcat tgattattgg acttagcgta ggcatcatag gcgccgtttt
    20161 tcttgcccaa aaaatgaaaa aagtactgct tggacttgaa ccacaagaaa tagcagaaca
    20221 gttaagtgaa aaaacgatta tcgaaaatga agtagatgaa ggaatcttag ccatctcttc
    20281 tgataaaact atcctcttga tcaatacacc tgcaaaaaac attttctacc aaacagagca
    20341 gctattggat ttcgaagtag gaaaaaaatc agtgataatc tatatgacgt tttgtttaaa
    20401 gagacgattg agcatcaaac gaccgttcgg actcaatcca tgtatttaaa tgggatagaa
    20461 actatttcta gcgttacacc tctttttagc aatgaccgtt ttttaggagc agtcgcaact
    20521 ttacgaaatc gatcagaagt aatgaaatta atggaggagc ttggggggac gaaacaatat
    20581 ataaactcca tgagagctca aacccatgaa ttcatgaata aacttcatgt catttctggt
    20641 ttgattgatt tgaagaagta tgaagaagtc acccatttta tccagcagct aaatcaaaat
    20701 tataaaaaag agattggcca agtctcaagt ctgatcaaag tacctgcaat tgccggtttt
    20761 ttattaggca aaataaacga agccaaagaa caatctgttg aaatgctgtt agaaagcgat
    20821 tcttttatcc ctaaaatgga gatggatgac tccatccatt cacttttgca agtgttaggg
    20881 aacttacttg ataatgcaaa agaagccgtg ctacaaaaac atgaaagaaa cggacaggtc
    20941 cggattcatt taaactatga cacagaagct gaagtattca tcgtgaccgt ctgcgatgac
    21001 ggtgtcggga tatcagaaga aatctcagaa aaaatattta cgctaggttt ttcgacgaag
    21061 ggaatcaatc gtgggtttgg attgaatctc atccattcaa tcgtcaaacg ccacggtgga
    21121 tttttagaat tttccaaaca aacaaatggc ggtacaatcg tctatctaga attaccaaaa
    21181 aaggaagatc aaaatgatga caaatgtcct gataatcgaa gatgatccaa tggttgcaga
    21241 attgaatcgt acttttgtgg aaagcatccc taatatgaac gtaatcgaaa gtttcagaaa
    21301 tgtagaagaa ggaaaaaaat atttcagaca aaaaaacgca gatctgctgc tgcttgatgt
    21361 atacatgcct ggcgaaagtg gtatccattt tttatcttgg cttagacagc aaggaaatga
    21421 tatcccagtg attttgatca ctgctgcaga tgatatcgaa accataaaga atgtgatccg
    21481 ctacggtgcc ttagattatt tgatcaaacc gttcactttg caacgttttc agctggcgat
    21541 cgaaaagtat cttgctttct ctcagacaat ccaaacgact gaaaaagccg atcagaaatt
    21601 gatcgatcaa gtattcaacc acaccactcc cattcacgaa ataccatcat tcatcgattt
    21661 gcctaaaggt ctgtcaaaac tgactttaaa aaaaataagt gcatttattc taaaagaaga
    21721 tactattttt tcaacaaaag aactggctca aaaaagctca ctgtcacgta tatccgtcaa
    21781 gaaatatttg aattttttag tagaacaaga tatattgaac gaaagaatca aatatttgga
    21841 aatcggccgg ccgctaacct gttatgaatt gacaaagaat gggcggaaaa agctcacaga
    21901 ttactgttga gtcatcgaat tatcccatac aaaatagtcc aagtatacag aagtaacaac
    21961 tgtttacttg gactattttt aaacatttta ttgacgaata tccatgtttt cttatcttaa
    22021 ttgacgggct ttaggaagag atacctcatt tttcgttttt ccagtttcta ttatttcacg
    22081 aaagttctcg aaacttgtcg aaatcatatt tgataatgcc tcatctgtat ttgaacctac
    22141 atgcggggtt acaagaactc tagggtacat ttctactaat tgctggatag cagggtctgg
    22201 aatcgtctcc catggcttga aggatcggaa aaaaacctct gtttcatttg caaaaacatc
    22261 ggttgcaaaa ccagctaaat gattgctttt caaagctctt aaaatagctt cattatcttg
    22321 aagctctcca cgggctgtat taatcaagat gctacctttt ttcatttgag ctaaaaatcc
    22381 ttcattgatc atttgatcgt tttcacctgg gatatgaggt acatgaatac tgacaatatc
    22441 gcttttggct aataactctt caagcgagtg gaacgtcaat acttttttag cagcttctgt
    22501 ctgatgaata tcgtagccga tcactttggc tcctagtccc ttaaacaaaa cagcttccgt
    22561 caaccctatc ctgcctgttc ctatgatccc tactgtgcaa tttctgattt cacgactaaa
    22621 catttgctca tccactcgaa aatctttgaa agatgttcgg atcgtcgtat acgcagtatt
    22681 tctcaaaagc atcatggcaa aagtcaaaga taactctgct atcgcatttg gcgaatacga
    22741 tggtacacgg gctactgtca taccaaaatc agctgccgct tgtagatcga tatgattgac
    22801 tcccacagtc cgtgtaaaaa cataacgaat cccatattcc tgcatttttg caagattggt
    22861 ctcatctgct atacaatttc ccctcaacaa tacagcatca tggtcctttg ctgacacgat
    22921 attgtcatgt gtcaacagtt cttcataaag actgagatca taatggtaaa tattcaattt
    22981 ttcaaaaaaa tctatctcgt tcggacgaac accgtaacat gcgattctca tactttcttc
    23041 ctccatttct tatgaaaata aacctatgcc atgaatcatt tgtaatacaa cgatccatac
    23101 aggtgtcagt accacagcaa aaatagttga cagtaaggat gcattggagg ctaaaagtgc
    23161 ctccctatca aaactgatag cataggctgc tgccactgtt gcagctggtg tagccatcat
    23221 gataacgatt gttgccagtg cttcaaaatt gactggtaga atattcgttg ccgtcaaaac
    23281 tgctagaatc acgatattca atgcaggtac aaataccact ttgactagac tgtaatacca
    23341 tgaagttttt tcagatgcag cttttttgaa gcttacttca cctaatgttg cgccgattgc
    23401 tagccacgcc aatggtgaac ttaaaccagc taaataatcc atcggctgaa atagccacgg
    23461 tgcagtctgg tcaattcgca aaaacgctac ttggcttatc tctccttctg cacctgtaac
    23521 tgacacttgt ggcagatacc cttggaacag ccaaatgaaa aggccggcaa aagtagcgat
    23581 aatgattgga ttgacgaaca tcgctttgat atttttagct tccatcttca agccactcat
    23641 ttttatgtat ccataagaat acaaaaagat tcggtagcca atattgaaaa ttgaggaata
    23701 catcacgccg acaggaccat aaatcgcact gacgataggc gttccgaaaa aggtcgtaga
    23761 accaaagatc gtcaggacac gcaacgttgt ttgcttatcc cctttatagc ttaagaaaaa
    23821 aggttttgaa actagaataa gaataatata aataactaat ccccaaatca agacattgat
    23881 tccttgcatc agtaattctg tattgatatc ttgcatgaaa gctcgaaatg ccagtgctgg
    23941 caaagccact gttaagacga ctttcgatag aatttttcca acagattcag aaaaaatccc
    24001 cttttttcta caaacaaacc ctaataatat aataaaaact gttgaagtga ttgcactgac
    24061 aatcgacata tctgtcaaag tcgatcgaaa aatgtcaaat aagttcatct ttctcctcct
    24121 aattatgttc atttattcac aataaaaaaa gcttttattt tgctaatacg tattaacatt
    24181 tttaatttta tctttacatt gttacttatt gaacaatagg aaattaaaga aacttttgaa
    24241 acttatgtaa agt
//
LOCUS       DADVXU010000034        23829 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.036, whole genome shotgun sequence.
ACCESSION   DADVXU010000034 DADVXU010000000
VERSION     DADVXU010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 23829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.036"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(53..787)
                     /locus_tag="KB953_001293"
     CDS             complement(53..787)
                     /locus_tag="KB953_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HBB6866281.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="KB953_001294"
     CDS             1114..1524
                     /locus_tag="KB953_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBB6866282.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="KB953_001295"
     CDS             1517..2218
                     /locus_tag="KB953_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HBB6866283.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="KB953_001296"
     CDS             2218..3804
                     /locus_tag="KB953_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6866284.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="KB953_001297"
     CDS             3823..4044
                     /locus_tag="KB953_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HBB6866285.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="KB953_001298"
     CDS             4041..4802
                     /locus_tag="KB953_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HBB6866286.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="KB953_001299"
     CDS             5182..5691
                     /locus_tag="KB953_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBB6866287.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="KB953_001300"
     CDS             complement(5766..6305)
                     /locus_tag="KB953_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HBB6866288.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="KB953_001301"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="KB953_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBB6866289.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7318..8169
                     /locus_tag="KB953_001302"
     CDS             7318..8169
                     /locus_tag="KB953_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBB6866290.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8197..8832
                     /locus_tag="KB953_001303"
     CDS             8197..8832
                     /locus_tag="KB953_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBB6866291.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="KB953_001304"
     CDS             8852..9625
                     /locus_tag="KB953_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBB6866292.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="KB953_001305"
     CDS             10033..10830
                     /locus_tag="KB953_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HBB6866293.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="KB953_001306"
     CDS             10808..11221
                     /locus_tag="KB953_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBB6866294.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="KB953_001307"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="KB953_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBB6866295.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="KB953_001308"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="KB953_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBB6866296.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRQTGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /gene="maf"
                     /locus_tag="KB953_001309"
     CDS             15998..16558
                     /gene="maf"
                     /locus_tag="KB953_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HBB6866297.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..18631)
                     /gene="treP"
                     /locus_tag="KB953_001310"
     CDS             complement(16643..18631)
                     /gene="treP"
                     /locus_tag="KB953_001310"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HBB6866298.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18854..19567
                     /gene="treR"
                     /locus_tag="KB953_001311"
     CDS             18854..19567
                     /gene="treR"
                     /locus_tag="KB953_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HBB6866299.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19644..20090
                     /gene="msrB"
                     /locus_tag="KB953_001312"
     CDS             19644..20090
                     /gene="msrB"
                     /locus_tag="KB953_001312"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HBB6866300.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGVFKSLVE"
     gene            20260..20862
                     /gene="ruvA"
                     /locus_tag="KB953_001313"
     CDS             20260..20862
                     /gene="ruvA"
                     /locus_tag="KB953_001313"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBB6866301.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20875..21876
                     /gene="ruvB"
                     /locus_tag="KB953_001314"
     CDS             20875..21876
                     /gene="ruvB"
                     /locus_tag="KB953_001314"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBB6866302.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21905..22483
                     /locus_tag="KB953_001315"
     CDS             21905..22483
                     /locus_tag="KB953_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBB6866303.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22535..23017
                     /locus_tag="KB953_001316"
     CDS             22535..23017
                     /locus_tag="KB953_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HBB6866304.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23134..23508
                     /locus_tag="KB953_001317"
     CDS             23134..23508
                     /locus_tag="KB953_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866305.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7780 a         4286 c         5115 g         6648 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtggactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agtgcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaca
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaagaaaatg gaaaattgct tcaccgggta accacaaaaa ggcattgaca ccattccaaa
    18181 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    18241 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    18301 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    18361 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    18421 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    18481 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    18541 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    18601 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    18661 tatgcaagtt gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat
    18721 taaaaagtac gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa
    18781 ataggaacat tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag
    18841 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
    18901 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
    18961 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
    19021 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
    19081 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
    19141 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
    19201 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
    19261 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
    19321 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
    19381 ggagatcaca gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga
    19441 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
    19501 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
    19561 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
    19621 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
    19681 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacgaccat
    19741 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
    19801 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
    19861 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
    19921 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
    19981 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
    20041 aaaagatgga tgaattaggt tatggggtat tcaaatcact tgtagaataa tggaaataca
    20101 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
    20161 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
    20221 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    20281 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    20341 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
    20401 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    20461 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    20521 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    20581 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    20641 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    20701 acaaaagacg ctacagctct atctgaagca atggaagctc tctcggctct tggttacagt
    20761 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    20821 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    20881 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    20941 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    21001 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    21061 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    21121 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    21181 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    21241 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    21301 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    21361 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    21421 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    21481 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    21541 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    21601 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    21661 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    21721 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    21781 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    21841 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    21901 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    21961 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaacgaag caaaaatcaa
    22021 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    22081 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    22141 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    22201 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    22261 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    22321 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    22381 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    22441 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    22501 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    22561 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcattaga
    22621 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    22681 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    22741 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    22801 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    22861 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    22921 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    22981 ttggattgat gaaaataaaa taacaaacaa tttgtaaagt taacgctttc tttttaaggc
    23041 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    23101 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    23161 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    23221 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa agtttattct tgagctgcaa
    23281 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcaaatcg tttgagtagc
    23341 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    23401 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    23461 ataacaatcc tttcaccagc aggggaacag gaacggttat caaaataatt tataccagat
    23521 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
    23581 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcatagcatg acagcattcg
    23641 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
    23701 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
    23761 gtatgcttat ataatagtca ttagttgtga atattttgtg acgaaagttt tcgaaagttg
    23821 ttaagtttt
//
LOCUS       DADVXU010000035        23694 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.037, whole genome shotgun sequence.
ACCESSION   DADVXU010000035 DADVXU010000000
VERSION     DADVXU010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 23694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.037"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(530..2026)
                     /gene="lysS"
                     /locus_tag="KB953_001318"
     CDS             complement(530..2026)
                     /gene="lysS"
                     /locus_tag="KB953_001318"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBB6866306.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(2137..3141)
                     /gene="dusB"
                     /locus_tag="KB953_001319"
     CDS             complement(2137..3141)
                     /gene="dusB"
                     /locus_tag="KB953_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBB6866307.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(3142..4029)
                     /gene="hslO"
                     /locus_tag="KB953_001320"
     CDS             complement(3142..4029)
                     /gene="hslO"
                     /locus_tag="KB953_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBB6866308.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4246..6357)
                     /gene="ftsH"
                     /locus_tag="KB953_001321"
     CDS             complement(4246..6357)
                     /gene="ftsH"
                     /locus_tag="KB953_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBB6866309.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6447..6992)
                     /gene="hpt"
                     /locus_tag="KB953_001322"
     CDS             complement(6447..6992)
                     /gene="hpt"
                     /locus_tag="KB953_001322"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBB6866310.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6992..8437)
                     /gene="tilS"
                     /locus_tag="KB953_001323"
     CDS             complement(6992..8437)
                     /gene="tilS"
                     /locus_tag="KB953_001323"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBB6866311.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8557..9027)
                     /locus_tag="KB953_001324"
     CDS             complement(8557..9027)
                     /locus_tag="KB953_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HBB6866312.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(9073..9498)
                     /locus_tag="KB953_001325"
     CDS             complement(9073..9498)
                     /locus_tag="KB953_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HBB6866313.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9565..9834)
                     /locus_tag="KB953_001326"
     CDS             complement(9565..9834)
                     /locus_tag="KB953_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HBB6866314.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9845..11440)
                     /locus_tag="KB953_001327"
     CDS             complement(9845..11440)
                     /locus_tag="KB953_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBB6866315.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11455..14976)
                     /gene="mfd"
                     /locus_tag="KB953_001328"
     CDS             complement(11455..14976)
                     /gene="mfd"
                     /locus_tag="KB953_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBB6866316.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14993..15553)
                     /locus_tag="KB953_001329"
     CDS             complement(14993..15553)
                     /locus_tag="KB953_001329"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBB6866317.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15906..16880
                     /locus_tag="KB953_001330"
     CDS             15906..16880
                     /locus_tag="KB953_001330"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBB6866318.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            17050..18450
                     /locus_tag="KB953_001331"
     CDS             17050..18450
                     /locus_tag="KB953_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HBB6866319.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18528..19250)
                     /locus_tag="KB953_001332"
     CDS             complement(18528..19250)
                     /locus_tag="KB953_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBB6866320.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19329..19835)
                     /locus_tag="KB953_001333"
     CDS             complement(19329..19835)
                     /locus_tag="KB953_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBB6866321.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19958..21757
                     /locus_tag="KB953_001334"
     CDS             19958..21757
                     /locus_tag="KB953_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HBB6866322.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            22251..23639
                     /gene="ltrA"
                     /locus_tag="KB953_001335"
     CDS             22251..23639
                     /gene="ltrA"
                     /locus_tag="KB953_001335"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBB6866323.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
BASE COUNT         6711 a         5100 c         4363 g         7520 t
ORIGIN      
        1 gagtgtttcc cgattcattt ttggctgccg tagcaacttt tatatcatat cagagagaca
       61 agcgattgtc aactcttttt taaaagtttt tttcgatttc gtttttcgaa atctttcggc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcgt
      241 tagtaacatg tcaataactt ttttgttatg tttttgaata aatattttct tctgtctttt
      301 ggacaagtaa taatatacca actaaattta gcttggtcaa cgaatttttt gtattttttc
      361 aagttttttt caataaacga ttattcataa cttttctttc tttatcgttc gttttcgtct
      421 tctttaatgt ctagaatagt cggcattagc ttttgaactt tcgttattat ttattccttt
      481 ctacacagca aaagctgcct aaggaattcc ttagacagct tttattcgat tatctcattg
      541 ttgggaatag caaaacatcc cgaattgatt gtgcatcagt tagcaacatt actaatctgt
      601 cgatcccgat gcctagacca ccagttggcg gcataccgta ttctaaggct tccaagaagt
      661 cttcgtctac accgtgagct tcgtcatttc ctagttcacg ttctttttct tgcgcttcga
      721 atctttctct ttgatcaatt ggatcgttca gttctgtaaa ggcattagca aattcgcgcc
      781 ccactatgaa tagttcgaat cgatctgtga atcgaggatc ttctggattt ttcttcgcta
      841 aaggcgaaac ttcgactgga tgtccgtata caaacgtcgg ttgtgtcaat gtttcttcaa
      901 caaatgtttc aaagaattca ttgataacat gtccgacacc catagcttcg gttacttcta
      961 cattgtgttt ttcggctaaa gctaatgctt cttccaatgt catttcttgc cagaagtcta
     1021 caccagtttg ttctttgatt gcatctacca tatggatgcg tttaaagtca ctttctaagt
     1081 cgacagcttg tccatcatat gtgatttttg ctgttcctag tactttttca gctgcgttac
     1141 ggataatacc ttctgtcaga ttcattacat cattgaaatc agtatatgct gtgtatgctt
     1201 ctaacatcgt aaattctgga ttatgtgtag tatcaattcc ttcattacgg aaaacgcggc
     1261 cgatttcata aactttttcc attccgccaa caattagtcg ttttaaatga agttctagcg
     1321 caatacgcaa atataaatca atgtccagtg cattatgatg cgtgataaat gggcgtgcgg
     1381 cagctccacc tgcctcattg tgcaatactg gtgtttctac ttcaatatag ccattgccat
     1441 ctaaatagcg acggatttcg ctgataattt ggcttcgttt cataaatcgg tcaaagcttt
     1501 cgcgattact gattaaatcc aagtagcgtt gtctgtagcg ttgttcaata ttcgttaagc
     1561 catggtattt atctggcagt ggacgaagtg cttttgacaa aatcgtgatt tctgaagctt
     1621 tgatagaaac ttcgcctgtg tctgttttca tgatttgtcc agttacacca aagaaatctc
     1681 caaggtctgc atgtttgaat acttcatagg cctcatcacc tacttggtct tttcttacat
     1741 aaatttgaat ttggccttca cgatcttgta gatgcgcaaa tccagctttc ccttttcctc
     1801 ggcgagtcat catacgacca gctacactag cggtcaatcc catttctgct agttcttctt
     1861 ttgttcttgg atcaaatagt tcatgcaatt cttctgagtt gtgcgtgcgt tcaaatcgtt
     1921 ttccaaatgg atcgattcca tcttcacgca attgttccat tttttgacga cgtacaagca
     1981 tttgatcatt taaatcttct acttgatgtt gttgttcctg tgtcacaaaa gttcctccat
     2041 tctcatgtta atctctttcc tataatgcca atgaaacaca aaaaaagcaa gccgaacttg
     2101 gaatttcggc ttgaaaattc acgaaaaact tagaaatcac aacgtttgtt tcaatagtac
     2161 tttttccgct ttttctacaa attgatccag caggtcattg atttcttgct gtttttctgt
     2221 ctgattgatt gctactttca ctttcgctgc tcgaggaatc ccttttaaat aataagccgc
     2281 atgtgttctg aattctctac aagctacttt ttctcctttg agatctgcta agcgttgtaa
     2341 atgaagttta gctgtagcaa ttttttcggc tggtgttggt tcaggaatca gttcccctgt
     2401 ttcgagataa tgctgtgtgc ggtgaatcat ccatggattg cccagcgctg cacgaccaat
     2461 catcacaccg tctgcaccga catagtcaag catccatttt gcatcttctg gcgttttgac
     2521 atccccgttc cccataaatg gaatcgtcaa atgttttttc acttctttca atacttccca
     2581 gtccgctttt ccttcataca tctgaacccg tgtacgtcca tgcatagcga tggcacttgc
     2641 tccagctgat tgtgctgcta atgcattttc tacagcaaag acgtgctctt catcccaacc
     2701 aatacgcatc ttgattgtta ctgggatatt tactgctgaa gaaacagctt ggaccatttc
     2761 gtatactttc tcaggatcta aaagccattt tgctcctgct tctgctttga ttactttatt
     2821 tactggacag cccatattga tatctattat atcggcagct gtgttttctt cgacaaattg
     2881 agcagcttca actagactat ctttatttcc gccaaatatc tgtaaactta atggatgttc
     2941 tgtttcttca atgtggagca tctctaaagt ttttttgttt ctgaaatgaa taccttggtc
     3001 actgatcatt tcacatacga ctaagcccgc accaaattct ttgacagtga ctcgaaatgc
     3061 tgaatttgtt attccagcca tcggtgcaac aacgacacga ttagggattt ccacattgcc
     3121 aattttccat gtgtgttcca cttaaatccc tcctaaggct tctttcttca atccatctaa
     3181 ttcttcttcg ctatacttgt atttgttatt acagaaagaa caaaccgctt ctgcaccgtg
     3241 atcttcgtct atcattgctt ggatttcttc tgcacctaac gcgataattg cttctgcaaa
     3301 tttttctttt gaacagtcac atttgaattg aacaggcatt ttttccaaaa tgttcaattc
     3361 ttctgtacca agcaatcctt tcaatatttc ttctggtgtc tgtccttcat ctaagagggt
     3421 agagattctt ggtgtcgttt ttaattgttc ttcgatcttg ctgattgttt cttcatcggc
     3481 acctggcatg atttggatca tgaatcctcc cgccgtcttg atgctgtcgt cagtattgac
     3541 caatacactc agtccaacag ctgaagggat ttgttctgag atagcaagat agtaagtaaa
     3601 atcttcagcg atttctccag aaacaatcgg cgtttgccca gaaaaaggtt cttttaatcc
     3661 taagtctttt gtgacagtaa acattccttg tgttccaact gctccccgca catcgatttt
     3721 accttcacta tttagaggaa gactgagatg cggatttttg atgtaacctt tcacatctcc
     3781 ctttccattt gcatctacta caattcctgt tgctggacca tcgccttgga ttcgaacagt
     3841 tagtttgtct tctcctttga gcgtcgcacc aagtagcaga ctaccgacca atgtccgtcc
     3901 caaagcagct gaggaggtgt gccaagtatc atgacgtctt tgtgcctccg ctacggtatt
     3961 tgttgcttga acggcgtatg ctcggacgaa tccgtcgtga gcaagtgcct taaccaaata
     4021 atctttcatt tgttatgtac tcctttttcc gtttttaaac tcatagtgaa tcatactagc
     4081 aactactgct tttttcaact ttggaatgct tcttttaaaa ataattcaga atcatctaga
     4141 aaacaggaca tcaccctatt tacaacttaa ataaagaaat ctcccgaaaa ccggtttttg
     4201 ttccggtttt cgggagtcat atcagatcaa ttgacctata tttttttatt tgtattgatc
     4261 gtctgaatga ttatcttctg attgttgaga atctgaatca tgttctgctt tattttcctc
     4321 attcagttca tgttttgctt cttcaaaatc acgtttttct tccgcttgtt tttcagcgtc
     4381 tttttcttcc aaagcacgtt ttgcttcttc aaaagttttc gcttcttttt cgcttggata
     4441 atcatttgac tcagtacctt caggcatcac accgtgttca aacaatgact tgattgcttt
     4501 ggcatccaat gtttcgaatt ccaaaagttt ttcagcaatc agtttatgct gtgcacgatg
     4561 agcttcgatg atttctcgag ctttatcatg agcttccatc aagatttttc gtacttcttg
     4621 atcaatctcg aaagcgactt gttccgagta agcttttgtt tgaccatagt cgcggccaac
     4681 aaacacttga tggtttcctt catattgaac aggacctaaa cgatcactca ttccatattc
     4741 tgttaccatg cttcgtgcta gagctgtcgc ttgctcaaag tcattggaag caccagttga
     4801 ttggacattg aagatgattt cttcagctgt acgtccacca agtaagccta cgatttgttc
     4861 aaacatatct tctttcgtca tcaagaactg atcttctttt ggtaaagcga tcatatatcc
     4921 gccggcgcgt ccgcgaggga tgatcgttac tttgtggacg acccgagcgc gactcaatac
     4981 taatccgaca atcgtatgac cagcttcgtg ataagcaacc atttcgcgtt cttttttgct
     5041 gattacgcga tcttttttag ctggacctgc aatcacgcga tcttccgctt catcgatatc
     5101 agaagcatcg attttctttt tatttcttct agcagctact aacgctgctt cattcaagac
     5161 attttctaaa tctgctccgg caaaacctgg tgtctgttga gccacaactt tcaagtcaac
     5221 gtcatctgcc atcggtttat tccgtgcatg gactcgcaag attgcttcac gaccttttac
     5281 atctggacgt ccaactaaaa tctggcggtc aaaacggcct ggacgtagta acgctgggtc
     5341 tagtacatca gaacggttgg ttgcagcaat gacgattact ccttcatttc cgtcgaagcc
     5401 atccatttct acaagcaatt ggttcaatgt ttgttcgcgt tcatcatgtc cgccgcccat
     5461 accggcacca cgttgacgac ctaccgcatc aatttcatca atgaaaatga tagctggtgc
     5521 gtttttcttc gctgtttcaa acaaatcacg aacacgactt gctccaacac cgacaaacat
     5581 ttctacgaaa tcagaacccg agatcgagta aaacggtact ccagcttctc cggcaacagc
     5641 ttttgctaat aaggttttac ctgttcctgg aggtccttct aaaagtacac ctgccgggat
     5701 acgggcacct aattcaacaa atcggcgagg atctttcaga aattcaacaa cttcaactag
     5761 ttcttgtttt tcttcttccg cacccgctac atcagaaaaa cgtacgcgat tagctttttt
     5821 gtctgcttct ttggcttttg atttaccaaa gttcatgaca cggccgccac cgccaccgcc
     5881 gccgccttgt tgtcccatca tcatgtaaaa gaaaaagatg atgattacga ttggcaagaa
     5941 gctaagcaat agggaaaccc atataccgct gcttgattct tcttttactg ttgtagcaac
     6001 tttgttttct tttgccagtt ctgttacttg gctcaatgtt gtatcatttg gcaaaatgat
     6061 tgttgtgaaa tgggtagtag tcgtatctgt tgaacctaag atagctaacc caccgctgtt
     6121 agcgacggtc tgctgttctt tgtattcacc agtgatctta tatacaccat ttgcaggctg
     6181 cacttcaaat tctttgatct tgcctgcttg tagttgttct gtgaattttg agtactcgat
     6241 atctgacgat cgctggccgt tattaccaaa aatgaaataa acgaccatta ccatcgctaa
     6301 gattacgagg acataataca aggcattttt catcatgccg ttgttctttt tgttcatagt
     6361 tgccctccct aagcgtcaat ttaatttaac agttttttat caatcctgac cattctattg
     6421 taccataatt taattgagat acagcattaa tttgattgat aaacactagg tttcaataca
     6481 ccgatgtacg gaaggttacg atatgcttct gcataatcca agccataacc gaccacaaat
     6541 tcatttggta cgttgaagcc aacataatct gcttcgatat ttactactct tccttcaggt
     6601 ttgtcaagta gtgtaacaat tttgactgat tttgctttgc ggtatttgaa aagatccact
     6661 aaataagcaa gtgttcttcc gctgtcaatg atatcttcaa cgatcaacag atcgcgacct
     6721 tctacattcg tatctaagtc tttgacgatt tttacttccc ctgacgagac tgttgcattc
     6781 ccgtagcttg aaacatccat gaaatccaat tccaaatacg tatcgataga acgaacgata
     6841 tctgccatga atggtaccgc tccttttaag acacctacta ctaggggatt ggtgttttgg
     6901 taaatttctg ttaactcttt gcctagttct ttgcaacgaa cttgtatctc ttcttgtgag
     6961 atcaaaattc tctcaatatc cttttccaac atcaggttct ccttccaatt gcttcttttt
     7021 gatatttgta aagaagtatg tagtgtattt tatcagtttc ttcacgaata ctcaaatatg
     7081 aaaaggcaaa aggtatcaag ctatatatat ttcttttggc atctgtaatg atccaagcat
     7141 gctgcctttg tgaattaggt actttctgat cgataaaata tctagatact ttttttgtga
     7201 gattctctgt taatttaatg cggtctcctg cttttctttt ttcgacatac agttgcacat
     7261 cagcaggaat ccaaatgtca tgagaaaata gagaccatga atctgttgct ttgatgtccc
     7321 ctgcattttc aggtttgacc agaccaatcc attctgtttc attcaaatat attccttgtt
     7381 ctggaatcag caacaagcgc tggtcttctg tctggctagt tgcttgtttt tcttttttag
     7441 agagtacaac aaaatgatac tctcttttca gtatccaatt attgccaata ctgagttgcc
     7501 attggctttt tttctgttgc cactggtcta aaatcgactc catttgttgt tctttcaaaa
     7561 taattcctgt ttttgagaat gctttgtcga aaaaggagta aaaaaaatag tatcgttcag
     7621 catctgtcat ttcttcaatc atttctgcag aaaatcgaaa ttgttcctcc cactgttcga
     7681 cttccttttc gatcaactgt cccatatatt tttgaatgac ttggtctgcc cattggatct
     7741 gttgcgaaaa ttgctggaaa tgtctcaacg cctgtgtatt ttcttgtttc aattgaggaa
     7801 ctaccaaatg acgaagccga tttctctgga tatctaacaa ttggtttgtc tgatcctcaa
     7861 agtaaacaag ctgcgattcc gctgcataag tatataggtc ttctttgcta aaactcagca
     7921 acggacgaat cagtctgccc gtagcaaatg gttgtacttc tttgattcca gaaaaagtat
     7981 ttaactgtcc cccacgaacg attttcatta acaccgtctc gatttgatca tctccgtgat
     8041 gagcagtcat caacgtgtca tattgtcctt cttccatcac gcgagtgaat tcttgataac
     8101 gaaagttacg agcttctgtt tctatggcgg atttggctgg atcttcccaa atacttacat
     8161 ataaagggag attcttctct tcacaatatg agcgaagata ggcagcttct tctgctgatt
     8221 cttctcttaa ttggtgattg atgtggacga tacctaaaga aaaaccgatt tggcgttgca
     8281 cttgtaaaag taaatggagc aaaaccatcg aatcaacacc acctgatacc gctgctaaaa
     8341 ttctcgatcc ttgttgccag aatccttgtt cattcccgtg ccgaataaat gactgctgta
     8401 atagcggtgc ttttttcccg cctttttcaa cgttcatcct ttccacaccc tttcttccta
     8461 aaaaaaagag agcgggaaaa cgtctaaaac gacaattaac ctgccctctg ttgatactat
     8521 gacccaaata gtcgcactat cattatttgc ggctttttag ctgcgacgtc cgccacggcc
     8581 accgcgcttt ccttctgtgt ttcgcttcaa tgaagataag cggtcatcgc tatctttcaa
     8641 aaatgaactc atcaatgaat cgaagtcttc tttttgagct gaaggttgac tgcgagaaaa
     8701 agattttttc tgtgctggac gatttcggtt gtcacgggga tctttttgtt gatatttttt
     8761 aggacggctt tcttctccag attcagcagg ttgttcctgc gctttccgga tagatagtcc
     8821 gatttttcca tcatcattta ctgaaataac tttaactgtt acttcatctc ctacacttaa
     8881 aacatcatgg atgtctttta catagccatt tgacacttcg ctaatatgaa ctagacccgt
     8941 ttttccagca cctagatcaa taaacgcacc aaaatttgtg attcctgata cttttccttg
     9001 cagttttgct cctacctcga ttgacataaa aaaaatgttc ctcctaatta tttccctttt
     9061 ttatattcct gcttattttg cagttgtaga actagttgat tgatctgttt catttgtcga
     9121 gctgtctgta tttgattcta atataggata aactttttca ccgtcttttg agtaaaagaa
     9181 tttgcttctg gcaagtttag ctacatactc atcatctttc agcaatgaaa catcttggtg
     9241 cagctgatca acgtctgcgt tgacttcttt catttcagta tttgcttcga ctctcaattc
     9301 gttcaattga tgcatccttt gcatgtcatg gaaaatctgc acacctatta tcgcgaaaat
     9361 gaccaaagca ccaacaaaaa ttgcagccag tctgcgtctt cgaaaaatca gttgacgttg
     9421 ttgttgctga aattcagcat actttttctt tgcatattcc gtatctagcg cagcgatttt
     9481 attgcttttt ttcgtcactt ggattcgctc caatcttttt gattgtcttt ttcattatac
     9541 caatatcgtg aaagcttgtc taatctattt ctctagtgtt tctgtgcgat tttcagaaat
     9601 aatttcgtac attttttgtg catcttcttt tttggttgat tcatgtaatt ccgtaatttt
     9661 cacttccaac gttttgttgc caaactggac tttcaaaata tcgcctactt tgacatcact
     9721 ggaagatttc gccaatttac cgttgatttg aattcttcct ttatcagcta cttcttttgc
     9781 tactgatcgt cttttgatga ttcgggaaat ttttaaaaat ttatctaatc gcatatctta
     9841 ttcctcactt tttaattttt ttatttaatc gcaggatttt cttaccaaaa ggcaacatta
     9901 gccattctcg tactgtaaac agacgaagac gaatcactgc ataaatgaac acagaagccc
     9961 cgatcccaac tcctatcacg ctgacaaaaa aagcagtgga acgatgagtt acaggaccaa
    10021 atactatatt caatacacca taatacagca gcagtactac tgtcatacaa agaaggctga
    10081 acatcaactt ccgtccaaaa tgccgttctt tccaaaaagc atttagctct tttgtttcaa
    10141 ttcgaacaaa ataccaaagc gtcacaccta atcctaaaag ggtagacagg cttgcacctg
    10201 ctgttcctaa taaaccggtc agaaaagaag tagtcaatcc ttttaccaat agtccccatc
    10261 ctgcgccttt taaagaaggg cgaaacgagt tcttgctttg agcaatactt tgataagctt
    10321 ggatgactgc agtgaaagca atggaaaaaa caaataatac aagcgctgca tttcccgcat
    10381 aatctttaaa caacgcataa ttgatataag ggagcagcaa ggctaatccc aaagacgcag
    10441 ctaatgctaa agcggtcgtc aatctaagat agattttggc tgtctgtaaa aactgacgat
    10501 agatccggtt ggtcaaatga cgagtcaacg caggcaggaa tgtcgcactt aatgcagtgg
    10561 caatcactaa acctaattgt actaaaggct gacctcgatc atagacccct tttgctatct
    10621 tcgcaccgta ttctgacagt ccgtaaactt ccaaagcatt ttttacaaag aaagagtcga
    10681 tcagctggaa aaagataagc aatccactat aaatcgaaac cagtccgcct tctaccagaa
    10741 aacgccggat caagggcttt gacggctgtt tgaacaatgg aaaccgtcgg aagcttaacg
    10801 ctcctccatg aatcttttgt tcatagtagt gaaggatccc atatgcgcat aaaccgccaa
    10861 aaacagctcc actcattgct gccgtccctg tttggtacac cgtccatcca agctttctaa
    10921 aagcaaacgc actacagaca atcacaaaaa cacgaacgaa ctgctcaact acttgagaaa
    10981 cagcagttgg ttccatcaag aatcttcctt ggaaattccc tcgataaaaa gatagaccag
    11041 gcatcagcaa aaaggtaaag gaaactactt ggatcagcgg tttcaattgt atgtctccca
    11101 tcatccaagc aatccagctg ctaaatgcaa agacaaacaa ccacaaaaag acacctgtcc
    11161 aaaatactaa gggataaaga ttttgcaaag cttgctttcg ctcgaaagga ttctttcttt
    11221 ctgcaacgta ctttgatatg aactgcggaa gaccggataa agctaaagtc attgctatcc
    11281 cataaattgg ataaacttgc tgataaacat aaaaaccttc gtcgccgact aagttttgga
    11341 ggggaattcg gtaaagggca cttaatactt ttgctatgaa agaagcaatc gtcaatacaa
    11401 atgctccttg catgacgcgc cgcatttctt tatgatccaa atcattgctc cttcctacgc
    11461 tgacagatat ttttgctcac gcaacgcttt gacaaacttt tggatttcct gaagccatac
    11521 tgcttcttcc atttggttgg ggatcgtcaa gcgtatcacc atctgttctt tttcaacgcc
    11581 tagactggct tttagttttg tcgctgtcag tgcttcgaat aactgttcca cactatagcg
    11641 ttttgttcca actttactta gagtaaagac tatatgcgcc tcctgtttcc ttatcgtttc
    11701 taggagcgct cgatcgccat ccattttgat ctgcccaatt gtcaagagat gtgctacttc
    11761 ttctggatat tctccaaaac ggtcgagcag atcatcttct agttcatcta gcatttcttg
    11821 cgaatcaagt tcacgaattc gtttatagat ctcgattttc tgccgttgat ctgccacgta
    11881 tgtttcaggt agatacgcat cgacaccgag gtcgatctca acagtggtct tctccacttg
    11941 actattcttt ccttgtttac gagaaacagc ttcgcttagc atttgagaat acatatcgaa
    12001 accgactgca tcaataaatc catgctgctg agcacctaag agattacctg caccgcgaat
    12061 ggaaagatcc cgcatcgcaa tcttgaatcc agatcccagt tctgtaaaat ctttgattgc
    12121 ctgaagacgt ttttcactga cttcgttaag gatcttctgc tgctcataca taaagtaagc
    12181 atatgcgaca cggctgcttc ttcctacccg ccctcgcaat tggtataacg tggataatcc
    12241 catataatca gcgttctcaa cgaacaacgt atttgcgttc gggatatcta ctcctgtttc
    12301 aatgatggtt gtagtgacaa gaacatcata ttgaccttca ataaattcaa aaagcgtatt
    12361 ttctaattgc gcctctgtca tttgcccatg agcataaccg atccgtgctt ccggaacgag
    12421 ttcttggatt tcttctactt tttgttcgat cgtttctacc cgattatata aatagaatac
    12481 ttgtccacct cgtcccattt ctcgatggat tgcttcgcga atagccccag gatttttttc
    12541 cataacatac gtttggatcg gataacgatt cgcaggcggt gtttcgatta ctgaaagatc
    12601 ccgcacgcct aacatcgaca tatgcaaagt tctcggaatc ggtgttgcag tcagagtcaa
    12661 aacatccact tgtgcacgta actgtttcaa tcgttcctta tgttttacac caaaacgttg
    12721 ttcttcgtca atgatcagta gacccaaatc gctaaattca atatcttttg acaaaattcg
    12781 gtgggttcct acaacaatat caacttgacc tgtacgcagt tgttcgatgg tttctttttg
    12841 ttgctttctt gttctgaaac gacttaatag cccgatattt acggggaaac cttcaaatct
    12901 ttccagcatc gtttcatagt gctgctgtgc caagatcgtt gttggaacta aaaaggctac
    12961 ttgtttgctt tcctttatcg ctttaaaggc cgctcgaagc gctacttctg ttttcccata
    13021 accaacatca cctacgagta gtcgatccat tggtttttct ttttccatat cccgtttgat
    13081 ctccgctgca cttctcaact ggtcatcagt ttcagagtag gggaaggcat tttcgaattc
    13141 tttttggtag gcgtcatcag gtccaaaagc atatcctttt tctgactctc ttgcggcata
    13201 taatttgatt agatcgtcag cgatatcctc gatttttgaa gagacttttc gcttggtttt
    13261 cgtccattca cttccgccta gcttattgat gcgaggcgct ttggcttcag aggcgacata
    13321 tttttggatc agattcagtt gagtaacagg aataaacagt ttgtcgtcgt tttgatacaa
    13381 aatagtcata tagtcttggt gaacgccatc tacttctagc gtttccatcc caatgtattt
    13441 cccgatacca tgattggcat ggacaacgta atctcctgct tttaactcat tatagctttt
    13501 caatcgctct gcgtttgtta ctgtttgacg tctcgtttgt tttttcgtcg ttttttggaa
    13561 aatttctttt tctgtgatag ctactatttt ttcttgagga agttcaaaac cagcctgcaa
    13621 agctccttca actaattgta cctgtcctct gatcaatgca tttttttctg tttctactac
    13681 taatatgtct tcatctcgaa gcatttgttc tgctttgcgt attctgtcct ttgtcggaat
    13741 aaagatcaca acagtctgtt tttgcttgcg ccaacggtcc atttctgttt tcagcaatgg
    13801 catttgtccg aagaactgtt gcatcgtccg atattgaaaa ttatggattg cttggaaacg
    13861 aagatttccc atcccttttt ggaagagtga gaaaaaggtt gtggtgaact tttccttctg
    13921 cacgattttt tgtatatctg ctgaaaatgt ctgctcagaa aacacccgca attcgctgat
    13981 tttttgagtc tgccattctg ccgcttccct ttcgatttcc cgatttgttt ccatgattcg
    14041 ctgataatca tcaacgacta gtaaactatt tgaagggaaa taatccaaga tcgtcacaga
    14101 ttcttggtaa agaagatctg agtagaattt tgcttcttct gtcgggacac ctgcttgcca
    14161 gcttgaagtc agctgaccga agtaatcttg caaaaactcg cggtctgctt cttctgtggt
    14221 cgcagcaagc cgtttttcta gcattttttg catttgctct attccatgtt ctagatcctc
    14281 tggtccgtag acttgttcac tcgttgggga aacccagatt tcttccgttt tttcgattga
    14341 gcgttgtgtt tctgcttcaa aataccgcat agaatctatc tctacatcaa atagttctac
    14401 acgaacagga taggttgtcg ttaacgggta aatatcgata atgcttcctc gaatactgaa
    14461 ttctcccggt tttgcgacca tctcttctcg tacatagccc atcaaagtca attggcgagg
    14521 aagattttcc acgtctatct catcaccaat atgccaattc aattgcgcct gtagccaagt
    14581 tttcttattc ggcagtcgtt tgtgcaaaga tgcgactggc aacaaataga tacctgcttt
    14641 tttttcttga agtgcgttaa gcgttgcaac tctctcgcct cgcgcttctg gagaagaaaa
    14701 ggccatttca gcagaaagca cttcatccac tggaaataaa tggatctgat cttgcggaat
    14761 cacatgttgc agatcttctg ctaactgatt cgcataatac aaatttggca tcagaataat
    14821 catgttttta tttgttttct taaacgctcg agcaaataga agtgttttcg ctgagcccgc
    14881 taatcctgtg attagttgtc taggcatttt ttcttctaaa ttgctgaccc attcttttac
    14941 ttgcggatct tttccgacta attgaataat atccattcct tttcctcctt cctcaattga
    15001 atcgattcat cgcatccacg aacgaatgcc cttctatgag atattcgact gctttaacag
    15061 attgacacat gctttcttct atcaaaggct gattttcttt gctgaaagga ctaagcacat
    15121 gctggactac tgtttttttc ccttctggcc tgccgattcc caccttgatt cgatcaaaga
    15181 cattcgtatt caaatgggaa ataatacttt tgataccgtt atgcccgccg gcacttccct
    15241 tttgacggag ccggattttt cccactgcca gatccaaatc atcgtagatt acgactaatt
    15301 cctctggata aatcccaaaa taagtcatta gtgggccgac tgcacgacca gattcattca
    15361 tgaatgtttg tggtttgact aaaaggatct tttctccatt atgaaaaaat tcggcaactt
    15421 ctgcttcgaa cggatttttt ttgaaagtag cttgatgctc gcgtgcaatt ttatccacga
    15481 ccataaagcc gatattgtgt tttgtatatt ggtatttggt acctgggttt cctaacccga
    15541 cgatcatttt catttttgtt ttccacctaa ttctaaaaag cagcacaaaa ggctggactg
    15601 ctaattagcg gtccagtcca tcgagctttt ttctttttta cacttgtcaa tgcatagtat
    15661 aacacaaaac aaggcgtttc ggctaaaaga gaatcgttaa taaatgataa agaaattaaa
    15721 aatttcttca aagtttaatg aaataattga tatataaaaa tagtgaaata acgcacatgt
    15781 aagcttttac atataataca accttgttat ctgttatatt ttatagccaa atagcgtgac
    15841 atggggcttg agaaatgtta gcataagcat gggaaaaaac aaatcaatgg aaggattgat
    15901 actaaatgac tgcagctgca gaaaaaaaag atcatcaaaa agttattctt gttggagacg
    15961 gcgccgtagg ttctagctat gcctttgctc tagttactca aaatatcgct caagaagtag
    16021 gtattgtgga tatcaatact gctaaaactg aaggagacgc aattgacttg tctcacgctt
    16081 tggcatttac ttcacctaaa aaaatctatt ctgctactta cgcagacgca catgatgctg
    16141 acttagttgt tattacagct ggcgcaccac aaaaaccagg tgaaactcgt ttagacttag
    16201 taaacaaaaa cctaaaaatc aaccgcgatg ttgtcacaca aatcgttgaa tcaggattta
    16261 acggtatctt tttagttgcc gctaacccag tagatatttt gacttactca acttggaaat
    16321 tctcaggttt ccctaaagaa cgcgtaatcg gttcaggtac ctctcttgac tcagctcgtt
    16381 tccgtcaagc acttgcggaa ttagttgacg ttgatgcacg taacgtccat gcttacatct
    16441 taggtgaaca cggagattca gaattcccag tttggtcaca tgcaaatgtt gccggcttgc
    16501 aaatctatga atgggtaaaa aacaacccag aaatcgatga agaagctatg gttaacctat
    16561 tctttggcgt acgtgacgct gcttacacaa ttatcgagaa aaaaggcgct actttctatg
    16621 gtatcgcagt agcattagca cgtatcactc gtgccatctt agatgatgaa aatgctgttc
    16681 ttcctttatc agtattcatg aatggcgaat atggattgaa cgacatctac attggtgcac
    16741 ctgctgttat caaccgtcaa ggtatccaaa aagtaatcga aattccttta agtgacagcg
    16801 agcaagaccg tatggctgct tctgctaagc aattaaaaga aattctagat gaagcttttg
    16861 ctaaactaga cgctgaataa tctttgaaca tgacggaaaa catctattcg gtgttttccg
    16921 tttttttatt ttatcaccct tatggtttct aaattttttc ataaagtaga gataaccttc
    16981 gtcttttcct ctaaattatg ctatcatgag accgttccac cctaaacttt tacttaagga
    17041 gttgtcgata tgacaagatc ttcgcatcga aaaaaatcga aacatgttgc tttgctttca
    17101 gttcttggtg ttctcttagt gctgatcagc ttttatacct atcgcagtac atactacaca
    17161 aagcattttt tgccgaatac agaaataaat ggaataaatg tcagcaatct aacagtcgaa
    17221 aaagcaaatg aaaaattaaa agaagcttat tctgataaaa aactggctat taaagaagat
    17281 ggaaaagtct ggcaggaagt accaaaatcc gagctcggct acaaagatga tttcacttcg
    17341 gagctttctc cgatcctaaa tgaacaaaac ggatggactt ggggaatgac ttatgtctca
    17401 gcggcagaaa aacaagaaat agatcctgtc agtcaaaacc accaaaaact agacactacc
    17461 gttcagacat tgacaactaa gctgactgat ttgaacaaag accggacacc tactactgat
    17521 gcaacgatcg aaaaagcagg tgactctttc gttatcaagc ctgaagtcaa tggaaatacg
    17581 atcgacgtcg atgctgcggt caaacaatta aaaagtgcgg taaactctgg taaagataca
    17641 attgaattga ctgaattcaa agaaaaacca aaagtaacat ctgaagacag ctcactgaaa
    17701 gaacaactcg catccatgaa tgctatcgca aatgtaaaag ctacttacag tataaacggt
    17761 gagactttcc agatcccatc aagtgatatt atgagttggc tgacctacaa tgatggaaaa
    17821 gtcgatttag atacagaaca agtacgtcaa tatgtaacag atttaggcac aaagtataac
    17881 acaagtacga atgacacaaa atttaaaagt acaaaaagag gagaagttac ggtcccagtt
    17941 ggcacttaca gttggacgat ccaaacagac agtgagacag aagcattgaa aaaagcgatt
    18001 ctggcaggcc aagattttac tcgttcaccc attgtgcaag gtggcacaac cgctgatcat
    18061 ccattgatcg aagatacgta tatcgaagtt gatctggaga accagcatat gtggtattac
    18121 aaagacggaa aagtggccct tgaaacagat atcgtttccg gaaagccaac tacacctaca
    18181 cctgctggag tcttctatgt ttggaataaa gaagaagatg caactttaaa aggaacaaat
    18241 gacgatggaa caccttacga gagtccggta aactactgga tgccgattga ctggacaggt
    18301 gttggtatcc acgattcaga ctggcaacct gaatacggtg gagacttatg gaaaacccgc
    18361 ggttcacacg gctgcatcaa caccccacca agtgtaatga aggaattatt cgggatggtc
    18421 gaaaaaggaa cgcctgtatt ggtcttctaa caaaaataat atataaaaag aaagcacccg
    18481 tacgattggt acaggtgctt tctttttatt atctaacaat gtgctactta catttcagca
    18541 gcagtaaaga cttctgaaac atcatcatcg tcttctagct tatctactag acgttccaat
    18601 tgctcttttt gttcctcatt taaatcaatc gtcgtttgtg gaatcatcgt caattctgct
    18661 tgcgccaaag tgaactcttt ttccaactca tctcgtacag cagcaaaatc ttctggtgca
    18721 gtataaatct cgaatacttc aggagaagtt tgcagatctt ctgcccccgc ctctaataca
    18781 tcctcaaaca tcgtctcttc atcaacagtc aacccatcgc gttcaatagc gatatatcct
    18841 ttgcggtcaa acaagtaact gactgaaccc gtttctccca gtgatccacc gtttcgagta
    18901 aaagctactc ggacatttgt cgctgtccga ttacggttat ctgtcaatgc atggactaag
    18961 attcctactc cacccggacc atatccttca tacgtgattt catcgtaatt cgtttcatct
    19021 acggcactcg tagccttttt aatcgcccgt tccacattgt cgttaggcat atttgcagct
    19081 tttgctttgt ccacaacaag ccgcaaagct ggattcatag atggatcagg accgcctgct
    19141 ttagctgcca tataaatttc acgagaaagt ttctgaaaga ctttccctcg ttttgcatct
    19201 tgggcatttt ttcttccttg gatattatgc catttactat gtcctgacat gatttttcct
    19261 cccttgttct tttgagaata cacttctatt ctagtactat ttcacaaaaa aacaaagcca
    19321 cagccaatct ataaacgctt cttgaatgcc gcaaaaagca cacataatat cacagcagtc
    19381 agagcaccga tcgaatccaa catgacatct tggaacatag gtgttcgtcc accggtcatc
    19441 atctgatgga attcgtccaa acctgcgtac cctgttgctg aaagccaagc aaggatcgct
    19501 gtcagccacc aaatttttag cttggggaaa acacctaaaa acaagctgcc tcccaaaata
    19561 aaataggtac cgaaatgtgc gcccttcctt aaaaagaact ctaaaaattt ggcgtagccc
    19621 agatggttga tactcaccat acttccacca taattaaaac gtatacccgc taatgtgtct
    19681 ttaaatggct gattaggcag ccagctttct aatcgcgaaa cttgtgattg ctgtccgtaa
    19741 gtttgagaag aactgtaaaa taagattata acgactatca gtgcgattac taaataaata
    19801 tttccgtttt tcatttgttt ttttgaagaa ttcataagtc acctctgtat tttatcttag
    19861 actttttttg aagaaacaac aatcattttc atagagactt cttccataaa aaagtgctat
    19921 acttaatttt gtattacaat atgaagggag ttttttcatg agttattcat taaattggaa
    19981 tctagattct attttttccg gaggcagtca ttctgatgct ttgaaccaac ggatgaagca
    20041 attggaagac caaatgaacg agtattatca ccgcgtaaca aaatggtcac ctagttctga
    20101 caatgcagag cagttaaacg ctattcttca attgcaagaa acgattacga atggttttac
    20161 ccaatgcagc agctatatta ctgcattgct ttctgccaat gtcaatgatt cagatgcaaa
    20221 ggtcttatca ggcaagcttt acgcaatgct gcctcgtctg caatcagccg aaactgtatt
    20281 gtcaaagaaa tttgcagaaa tatctgacaa tgattggaat cagatgcttt ctcaatcttc
    20341 gtttgaaaca attgcttttc gtttaaacga gattcgtcgt gatggctcac aattattatc
    20401 agaagctgaa gaaaacatta taaatacgct ttctttagat ggtctaaatg cttggagcag
    20461 tcattatgat acgattgtag ccagcatttc gattcccttc gaacaagatg gacaagtagt
    20521 cgaattatct gctggacaag ccttcaataa gatgatgggc gatccagatc caaaagtcag
    20581 agaaacactt ttcgcagcat gggaagaagc ctggaaagaa aaggcacccc tcttcacgga
    20641 tactttgaat catttagatg gttttagact ttctgactat gaacttcatg gtgtcacaga
    20701 ttttctacaa aaaccattag agtacaatcg gttgaagaaa gaaacattgt ctgtcatgtg
    20761 ggatacaatc cagaaaaaca aacaacccct tgttaattac ttgactagaa aagctaatct
    20821 tttcggtaaa gaaaaaatgg aatggcaaga tcaagatgca cctattattt taggcgattt
    20881 gaaagaaaaa acctttagtt ttgacgaagc agcagcattt attatcgaaa actttaacaa
    20941 attcagtcca aaaatggcta aatttgccca atctgctttt gaaaagagct ggatcgaagc
    21001 agaagatcgt cctgggaaac gtcctggtgg ttattgtact gagttaccag aaacaaaaga
    21061 atcaaggatc tttatgacct acagtaattc tgttaatgag gttgcgacac ttgctcatga
    21121 actaggacat gctttccata gcagcacgat gtgggattta ccttctttaa atcgcgaata
    21181 tgccatgaat gtcgcagaaa cagcaagtac attcgcagaa ttgatcgtag ccgatgctac
    21241 attaaaagtc gcaaaaacaa aagaagaaaa aatcaatttg ctagatacaa aactgcaaaa
    21301 tgctttagct atgttcatga atatccattc ccgattcatt tttgaaaatc gattctatga
    21361 agcacggcaa gaaggattag tctcagaaga taagatcaca gagatgatgg ttgaggcgca
    21421 aaaagaaggc tatcaaggag ctttagctac ttaccatcca tatttctggg ctgcaaaatt
    21481 gcatttcttt attgatgacg taccgtttta taacttccca tacacatttg gctacctctt
    21541 tagtttgggc atctatgctt acgccaacca aaagggagca aattttgaag aagaatatat
    21601 ccatttacta cgcgatactg cctcaatgac gacggaagat cttgcgaaga aacatttgaa
    21661 tgtcgatctt acccaaccag atttttggca agcaggtatc gacatggtga ctgaagatat
    21721 ccaaacgttc atggaattga cagaagaatt tatctgattt ctattttatc acaataaaaa
    21781 aagagttagt ccagaatggg ctaactcttt ttgatgtgcg ccctgcatgg gtgaaaactt
    21841 ggtggtgaaa gtccactaca ggcttggcag taggaactgt tagcgaaaga caaggcggca
    21901 atcgtgaggt tgtggctgaa ggaagtcgga cgcaaactcc tgaactgacg aacagaaact
    21961 agatacaagg ctgaattagg tcggataagt cttcatcaca agacaaagtc caatactgcc
    22021 cgaaacctat acagtaaatc tagcagttac atgggaggaa agttttcact cttaccgggg
    22081 gagatctcat gagggtgttg tgggcgttga atgaaaacaa accagcacaa cgatcatggc
    22141 gacatgatgg tgaatcatga gaagtcagcc gaggtcatag tagtaacgac actgaccgtt
    22201 acgaaggact gaacaataag aatcttgaat aatttaggag gtgtgagaag atgcgacgac
    22261 cgcagaaaac aagaaaactt ggctatcatc aaagagctag tgtggaacac gaagggtatg
    22321 atgaagtgcg cagcacttct tgcggtgaac agaggaaaca agatggtgta gaaaatctgt
    22381 ttgggagaat tctagatcgg aataatctca atcaagcgta tcttcaagta gtcagaaata
    22441 aaggggcggc tggtgcagat gggatgaccg tcgatcaact gcttccttac ttgaaagagc
    22501 accgcgagga attactatcc aatttacgcc aagggaacta taaacctcag cctgttttgc
    22561 gagtagaaat accgaaacca aacggcggtg taagaaaact aggaattccc acggttattg
    22621 atcgtatgct tcaacaggca atcaatcaag tgttacaacc gctatttgaa ccagaattct
    22681 cagataatag ttatgggttt cgcccaaatc gcagcgcaca tgacgcagtg aagaaagcaa
    22741 aatcttatta tgaacaaggt taccgatatg tggtggacat tgatatgaaa gcctactttg
    22801 acaccgtaaa tcacgataag ctgatgtatt tcattgagcg gaaagtaaag gacaaacaag
    22861 tcctaaaact gatccgtcaa tacttaatga gtggtgtaat ggaaaatgga ctcatcacaa
    22921 caactgaaga aggaacgccc caaggaggca atctctctcc cctgttaagt aacatctacc
    22981 tgaacgaatt tgatcaagtg ctagaaaatc taggacataa atttgttcga tacgccgatg
    23041 actgcaatat ctatgttaga agcaaacgag caggacagcg tgtgatgaag aaatccattc
    23101 aatttctcga agaacaacta aagttgacgg taaatcgaga aaagagtgcg gtaggaagtc
    23161 cattgaaacg aaagtttctt ggattctgca ttctgccaac aaagaaaggt attaaaattc
    23221 gccctcacgc aaaagcaaaa cagcgtgtga aaagcaagct caaacaactc actaaaagaa
    23281 accgtggaag aagtatccaa cttattttaa aagaaatcaa gcaactaatg actggttgga
    23341 tcaactacta tggaattggt gagatgaaag aattcatgag ggagttaaac ggttggttga
    23401 agcgaagaat cagacaatat atttggaaac aatggaagaa tccaagaacc aaaagaaaaa
    23461 atctgattcg tttagggatc gataaagcga aagcgtatga atggtcaaat acaagaaaag
    23521 gtatttggtc agtctccaaa agtcatattc ttcatcgatc attaacagac aaagaactgg
    23581 cccgccaagg atatgaggac atctccttga agtaccagtt cgtacactca acttattgaa
    23641 ccgccgtata cgggtccgta cgtacggtgg tgtgagaggg gcggaaggtc agtt
//
LOCUS       DADVXU010000036        23653 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.038, whole genome shotgun sequence.
ACCESSION   DADVXU010000036 DADVXU010000000
VERSION     DADVXU010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 23653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.038"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(26..601)
                     /locus_tag="KB953_001336"
     CDS             complement(26..601)
                     /locus_tag="KB953_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBB6866324.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(606..1580)
                     /locus_tag="KB953_001337"
     CDS             complement(606..1580)
                     /locus_tag="KB953_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleosidase"
                     /protein_id="HBB6866325.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIASALKVNPNIGKHMERFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEEAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(1604..1648)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1863..2831
                     /locus_tag="KB953_001338"
     CDS             1863..2831
                     /locus_tag="KB953_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBB6866326.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            2928..3221
                     /locus_tag="KB953_001339"
     CDS             2928..3221
                     /locus_tag="KB953_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBB6866327.1"
                     /translation="MRSAAYGNQRRKNRLALFNDEQQEIGEMTWSDAGPDIMIVDHTF
                     VDPAYRGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            3339..3575
                     /gene="secG"
                     /locus_tag="KB953_001340"
     CDS             3339..3575
                     /gene="secG"
                     /locus_tag="KB953_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBB6866328.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3678..4433
                     /locus_tag="KB953_001341"
     CDS             3678..4433
                     /locus_tag="KB953_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBB6866329.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            4436..6793
                     /gene="rnr"
                     /locus_tag="KB953_001342"
     CDS             4436..6793
                     /gene="rnr"
                     /locus_tag="KB953_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBB6866330.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            6815..7279
                     /gene="smpB"
                     /locus_tag="KB953_001343"
     CDS             6815..7279
                     /gene="smpB"
                     /locus_tag="KB953_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBB6866331.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            7581..8300
                     /gene="atpB"
                     /locus_tag="KB953_001344"
     CDS             7581..8300
                     /gene="atpB"
                     /locus_tag="KB953_001344"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBB6866332.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            8353..8568
                     /gene="atpE"
                     /locus_tag="KB953_001345"
     CDS             8353..8568
                     /gene="atpE"
                     /locus_tag="KB953_001345"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HBB6866333.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            8659..9183
                     /gene="atpF"
                     /locus_tag="KB953_001346"
     CDS             8659..9183
                     /gene="atpF"
                     /locus_tag="KB953_001346"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBB6866334.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            9170..9712
                     /locus_tag="KB953_001347"
     CDS             9170..9712
                     /locus_tag="KB953_001347"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBB6866335.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9735..11291
                     /locus_tag="KB953_001348"
     CDS             9735..11291
                     /locus_tag="KB953_001348"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBB6866336.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            11305..12207
                     /locus_tag="KB953_001349"
     CDS             11305..12207
                     /locus_tag="KB953_001349"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HBB6866337.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            12230..13636
                     /gene="atpD"
                     /locus_tag="KB953_001350"
     CDS             12230..13636
                     /gene="atpD"
                     /locus_tag="KB953_001350"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBB6866338.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            13650..14072
                     /locus_tag="KB953_001351"
     CDS             13650..14072
                     /locus_tag="KB953_001351"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HBB6866339.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            14541..14771
                     /locus_tag="KB953_001352"
     CDS             14541..14771
                     /locus_tag="KB953_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HBB6866340.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            14868..16169
                     /gene="murA"
                     /locus_tag="KB953_001353"
     CDS             14868..16169
                     /gene="murA"
                     /locus_tag="KB953_001353"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBB6866341.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            16182..16358
                     /locus_tag="KB953_001354"
     CDS             16182..16358
                     /locus_tag="KB953_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBB6866342.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            16493..16789
                     /gene="yidD"
                     /locus_tag="KB953_001355"
     CDS             16493..16789
                     /gene="yidD"
                     /locus_tag="KB953_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBB6866343.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            16853..17320
                     /locus_tag="KB953_001356"
     CDS             16853..17320
                     /locus_tag="KB953_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB6866344.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            17405..18289
                     /locus_tag="KB953_001357"
     CDS             17405..18289
                     /locus_tag="KB953_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBB6866345.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLIGAAANYLAVRQGEN"
     gene            18403..18639
                     /locus_tag="KB953_001358"
     CDS             18403..18639
                     /locus_tag="KB953_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HBB6866346.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            18727..18807
                     /locus_tag="KB953_001359"
     tRNA            18727..18807
                     /locus_tag="KB953_001359"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18761..18763,aa:Tyr,seq:gta)
     gene            18814..18885
                     /locus_tag="KB953_001360"
     tRNA            18814..18885
                     /locus_tag="KB953_001360"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18846..18848,aa:Gln,seq:ttg)
     gene            19011..19181
                     /locus_tag="KB953_001361"
     CDS             19011..19181
                     /locus_tag="KB953_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866347.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            19401..20108
                     /locus_tag="KB953_001362"
     CDS             19401..20108
                     /locus_tag="KB953_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBB6866348.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRRDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(20167..20811)
                     /locus_tag="KB953_001363"
     CDS             complement(20167..20811)
                     /locus_tag="KB953_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HBB6866349.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKNFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSANHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            20938..21225
                     /locus_tag="KB953_001364"
     CDS             20938..21225
                     /locus_tag="KB953_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866350.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            21333..21653
                     /locus_tag="KB953_001365"
     CDS             21333..21653
                     /locus_tag="KB953_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBB6866351.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIA
                     LIGE"
     gene            21971..23047
                     /locus_tag="KB953_001366"
     CDS             21971..23047
                     /locus_tag="KB953_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBB6866352.1"
                     /translation="MFDQPEAKPREVVFFWTGQLTEKQTEIAKRILCHSEKRHHLIWA
                     VTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNELFLRYRYVFPEEKITLLHGNTA
                     TAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYNGDAGLAHAVQTAIRSNGRFIY
                     LSATPDEKLLKEIKQTFELHKLALRFHRRLLPEPVLVFMNNWRQKCGNKKKINSLVRI
                     IQQLIKENRILLFCPSITMMHRLKITLQEAFPEYEITDVSSQDDQRAKKVQKMREAIY
                     EILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQIAGRADRKGPYTNSRVYFFYE
                     EKTSAIRKACQEIKEMNEEGKQML"
     gene            23044..23653
                     /locus_tag="KB953_001367"
                     /pseudo
     CDS             23044..23653
                     /locus_tag="KB953_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
BASE COUNT         7820 a         4125 c         5092 g         6616 t
ORIGIN      
        1 attttgctaa tccagctctg tttattcagc ctatttttct ttttgaatat atcaagcgtt
       61 gtggcaacat agtgatgact aatgaaacaa cgacgataga caacaaacga tcaaggtagt
      121 ctgtacctgc ctgtaccaag atcacacttg ttgtctgact taaacctaac ccatgtaata
      181 gctgaacgat catactggat cctgaggagg tgatcccgcc aaataactga acggtaatga
      241 tcgaagaaac gatcgttcca ggtaccgaca aaatcaaagc gagtaataac agtttcaagc
      301 tgtttttatc tttgccgaga tatcttggca aaatcaatcc agcaagcaag cctgtgagaa
      361 tttggattgg cgaataatac agagaaaaaa tatctgtagt cactccactt aaaataccgc
      421 tggacaatcc tgtcagcatt ccaggtacag gcccaagcaa tgcaccagca aaaatcgttc
      481 cgatagaatc gagatagatt ggcaaacgaa gccataaagc aatcgttcct ccaatataat
      541 tgatcccaat acacaaagcc atcactgtaa gtacgcgagt tgccattttg tttttattca
      601 ttttgttagc ccatctttaa cctttccaaa tctttcataa tcgtttcttc ttgtgcattt
      661 aaaagaacgg ttaaaaactt tcggaaaaaa agacgagtat ctacttttgt cataatttta
      721 ctgttagcag gtttctgcca aaattcataa cgatcaacca atgtttgtcc acgacttatt
      781 ccttgagttt ccacagcagt gaagcttttg aaaccttcac agatggcctc ctcgataaaa
      841 taagcaacag ctaacggatc attgatgaca catcctaaaa ttctttcttg tttccagtgg
      901 aagtcaaaat aaaaacgagt gatcgctttt agatactctc cctcttcagg attcatttga
      961 cagcagtatt cgaggatagt cggtgtcaag acgatttctc ttgtcacatc taacccaacc
     1021 atttctatcg tttgtttgag attctcgaat acataagaag ctgcttcagg atcacaccag
     1081 taattgtatt cagcaactgg cgaacagtta ccatggcttt tgtaagttcc acccatcgag
     1141 acaaaacgtt ccatatgttt cccaatattt gggttgactt tcaaagcaga agcgatattg
     1201 gtcaaaggac ccaaagcaat gactgatgta tttgtctttt ctttgaaata atcagccaaa
     1261 aaatcaacgg catggagagg ttctgcctgt tttctcaaga tcatcgggaa atttgtttcc
     1321 cctaaaccat ccataccatg agtgtcttgt gcactgatga aaggttgctt caaaggtttt
     1381 tccattcctt gataaacagg aatgtctaaa cgatcacagc gttccaggca tttcagcaca
     1441 ttctgcgtac ctatatctgc agggacattc ccacaaacta cggtaatagc aactacttcg
     1501 atatcagggg atttgacagc taatgataaa gctaaagtgt cgtcgatccc tggatcgcaa
     1561 tcaataatta cttttcgcat tatttccaat ctcctttctt tagagatact tagtttttta
     1621 ttctgggaag tttccgaacc agtccaagac gagcgtcttg attataatgc acgaaattag
     1681 tatactggct tttattgcga atgtaaatgc tctcttacaa gtaaatctat tgaggagaac
     1741 tttaagtaaa tgatacaatg aaaaagaaat ggaatttacg tgtgccgaaa ataaagaatc
     1801 ctatggatcg gtacattgga taatagatag attatagttt ggcagaaagc ggcgggtgta
     1861 caatggttat tttttatttc attcttattc tgataattat agggatcagt taccattttc
     1921 tttcttattt ttatgcaaag aaattagtaa gagcgggagt acagagagga tatgactggt
     1981 atgaagacac tggtcatcgt ttgatggacc ctgctgcagt cacgtcagta gaaaaaaaac
     2041 gacaaaaatt agaagaagag tgtgaaagct tttgggagca gggaacaccc aaaacaatca
     2101 aaagttatga cgggttgaaa ctagctggtc agatgttcat ccatccaaat aagcagaata
     2161 aatgggtcat ttgcgtacat gattaccgaa gcacagggaa aagagacatg tctcatatag
     2221 gtaaaagata tgcagagaaa ggattcaacg ttcttgtacc tgatttacgt gcgcacggag
     2281 aaagtgaagg agaaatcatc ggaatgggat ggctagatcg gctagattta attgcctgga
     2341 tccaactcat cttagatgaa cagccgaatg cgtccattat tttacacggc ggatcaatgg
     2401 gagcttctac gatcatgatg gcaagtggtg aaaaacttcc atcagcggtc aaaggattta
     2461 ttttagacag cggatatgtt tctgtttatg cagaattccg atatatgtta agtaaaatca
     2521 ctgtctttcc taaaaaaatg gtcatgagat atgcgaatca ttatgcccag aaatacgccg
     2581 gttactcctt aaagcaagca tcagctacga gacagcttgg cagcaatcat ctgcctttat
     2641 tagtcatcca tggtgaaaga gatcattttg tccctacaga agcagcgtac acgattcaaa
     2701 atgctacggc aggggataaa gcactgctgc ttgttccgga agcagaacac ttggaagcat
     2761 cgatgaaaga tccgaaaacc tattggacag tgattttttc atttattgaa caaagaataa
     2821 cgatgaatta attttctctt cttttttgta agtaattcga ggataaataa ttgtttatag
     2881 atcaggagac tgctaaaata aggtaggaat aaaaaaatat cagttatttg aggagtgcgg
     2941 catatggaaa tcaaagaaga aaaaatcgtc ttgctttatt caatgatgaa caacaagaga
     3001 tcggagaaat gacatggtca gatgcgggcc cagacatcat gatcgttgac catacatttg
     3061 tagatccagc atatcgtggt caaaagttag ctgaaaagct agtgtatact ggagtagaat
     3121 tagcaagacg agacgggaaa aagatcattc ctttatgccc ttatgcaaaa aaagagtttg
     3181 aaaagaaacc agaatatcaa gacgtcttac gtcagtcata agaataacag ctgaaagttt
     3241 tgaactttca gctgttattc catttatata gagttgtcac ggacttttat ctatgataaa
     3301 gttaaagaga cgatatcttt tgggaaggag gctgcgatat gtataattta attttgggaa
     3361 tcgtgatcgt tttatcgatt gttatggtca ttgcgattat gatgcagcca agcaagcaaa
     3421 atagtgcagc gagtgcattt accggcggtg cggatcaact attcggcaaa caaaaagctc
     3481 gcggctttga agctgtcatg cagcgatcaa ctgcagttat gggagctatc tggatgatct
     3541 tattatttgt cctagcatat ttatcttcga agtagttgaa taagcctctc gcaaaacggc
     3601 gggcggcttt ttttattgca aagagaacgc ttaagaagaa gttatgaaag aaactatggt
     3661 aaaatgaggc gaaagaggtg agacgagtga gacaattacc aaaacccctt tatgcaaaac
     3721 atggaaagcg agcggttttg ctgcttcacg cttactcagg aagtccaaac gatgttcgaa
     3781 tgctcgcacg atttttggaa aaagcagatt atacagttta tgcgccgctt tttaaaggac
     3841 acggaacaat ggagccatat gatatcttgc aagaaaaagc agaaagctgg tgggcagaca
     3901 ctaaaaaagc catccatttt ttacagtctg agcagttttc tgatattgcc gtattaggat
     3961 tatcaatggg cggcatcttt gctgtacgag cactagaaga agaatcagtg attggcggcg
     4021 gttttttctg ttcgcctttg tctcctgtaa aaacaaacgt gcctgaaaac tttgaaaaat
     4081 acgtacgtca agtgctgaag acagcaggta aatcagagaa agaaatcaat gaaaaagcag
     4141 ttgcttaccg ctcgttagct gaacaacaat taatggatat acaagatcag gcagcaatcg
     4201 tagaaagtag gttgtcagat atccaacagc cgatattttt agctcaagct gggaaagatg
     4261 aaatgatcga ccctaatggc gtatttgaaa ctgcacgcaa gttgagccgt cagcgagtga
     4321 cacttcaatg gtatcctgaa agtggtcatg tgattacagt tggtacagca agacgcgaat
     4381 tagaaaaaga cgtcttggaa tttttagaaa aattaccttg gaatgaggaa taaatatgac
     4441 aaaagaaacg ataaaagaga aaattctctt tttcatggaa agcagtcgca aacgcagttt
     4501 ttctatggaa gaaatcgctg aaggtttagg gttccaaaaa agcgatgatt ttaaattact
     4561 agtacaaacg attgctcaaa tggaaagaga acagagtata gttttcaata aaaaaggaaa
     4621 agtgaaactt cctttaaaac caatcctgat cgaaggaaca tttcgagcaa atgaacgagg
     4681 atttggattt gtaacgattg atcccgaaga agatgacatc tatatcccaa aagaagcgac
     4741 tggttttgca atggatggag acaccgtagc aatcgatatc atgaagcgag caaatacaga
     4801 tatggatcgc ggagcagagg ggaaagtagt cgagattcga gaccgggcga ctactcagtt
     4861 agcaggggaa ttcgttgctt attcagaaga agaaatacaa gaaacagatc tttacggcgt
     4921 tgttatcccg aaagatagaa agctcaacca atttaaagta tatgcagcag cagagggagt
     4981 tcgtccagtt gacggaagta ttgtgctgat cgaactgacc cattaccctg aaaaagggta
     5041 tgcgactagt ttagaaggga taatcaagca agtgattggt cataagaatg accctggcat
     5101 ggatatcctc tcaattgtcg tggctaatgg tatccctacg aaatttcctg atgatgtatt
     5161 gtctgaagca gatgaagttc cagatcaaat cagcgaaaag gatataactg gacgtagaga
     5221 tttaagaaat cagctcattg ttacgattga tggagaagat gcaaaagatc tagatgatgc
     5281 agttacggtt aaaaaattag ataacggtaa ttattttctt ggagtacaca ttgcagatgt
     5341 ttcttactat gtgacacaag gcagtcaatt ggacagagaa gcatatgaaa gagggacaag
     5401 tgtatatttg actgatcgag taattccgat gatcccgcaa cgtttatcga atggtatctg
     5461 ttcactgaat ccccatgttc caaggctgac catgagttgc gaaatggaga ttgatccaaa
     5521 tggacaagta gtctctcatg atattttccc aagtgtgatc caaacaacag aacggatgac
     5581 ctacacagct gtcaatcaaa tcttagaaga tcaagacgaa gaaacgatgc aacgctatgc
     5641 tcagctcgta cctatgttcc aagaaatggg tgaattgcac cgaatccttg aaacgatgcg
     5701 aatcaaacgt ggcgcgattt cttttgagga tcgcgaagcg aaaatccttg ttgatgctga
     5761 aggacaccca aatgacatcc agctaagaag cagaggaatt ggtgaacgct tgatcgaatc
     5821 tttcatgctt gcggccaatg aaacggtagc tgaacattat aataaattaa atctgccgtt
     5881 tatttaccga atccatgaac agccaaaaga agaaaaaatg cagcgcttct ttgattttgc
     5941 ttcagctttg ggcattctgg tccgaggaac gaaaggaacg atcacaccaa aagatttaca
     6001 gcatgtgatc gaaggagtag aagacaaacc agaagctgca gttatcaata ctatgttgct
     6061 aagaagcatg caacaagcac gctattcaga agacaatttt gggcattatg gcttagcggc
     6121 agattactac acacacttta catctcctat tcgacgctat cccgatctaa tcgtccatcg
     6181 tctgatccgt tcttacagtg aagataaaag tgaaagcaat caaagttcgt gggctgcgga
     6241 acttcctgaa attgcagatc atagttctaa gatggaacgt cgtgcagtag aaacagaacg
     6301 tgaagtagat tccatgaaga aagcagaatt catgatggac aaagtgggag aagaattcga
     6361 tgggatcatc agctctgttg tgaaattcgg cctgtttatc gaactgccga atacagtaga
     6421 gggattgatc catatcaatg aattgaaaca ggactacttc cattttgttg aaaatcattt
     6481 agctcttgta ggtgaaagaa ctggtctgac ttttaaaatc ggacagaaag tgcgagtaaa
     6541 agtagtcaaa gctgatccag atgaacgagc agtagatttt gaacttgttt ccgcagaaga
     6601 ggttacagcg ttggaacggc caaaaacacg aaatggaaaa tcaggaaaac gcgaaggaaa
     6661 acaagcagga aaacgcccag aaagaaatgg caagaagaac aaacgatata gcgatagtaa
     6721 gcaaacgttc ggtaagaaaa aagggaagaa gccattttac aaaggtgtta aaaagaagaa
     6781 aaagaaaaaa taaaatgagt tggaggaaac agctatgcca aaaggcgagg gaaaattaat
     6841 tgcacaaaac aagaaagctc gccatgatta ttcgatcatc gacacgatgg aagcagggat
     6901 ggtcttgcaa ggaaccgaga tcaagtcgat acgaaacagc cggatcaatc taaaagatgg
     6961 atttattcgc gtccgcaacg gagaagcttt cttgcataat gttcatatca gtccttatga
     7021 acaaggaaat atttttaatc atgatccgtt gcgcacgaga aagttattat tacacaaaaa
     7081 acaaatcatc cggcttgaaa atgaattgaa aaatactgga atcactgttg ttcctttaaa
     7141 agtctatatt cgtaacggct atgccaaggt attgattggt ctggcgaaag ggaaaaaatc
     7201 ttatgataaa cgggaagatt tgaaacgaaa agatatcgat cgacaaattg atcgaacatt
     7261 aaaaaatttc tctagataat ttcattatcc tttttcgcat attcggtgct tttgctttta
     7321 cttaatgata aaatttgtta gcatttaatc tgataaaaca tgattatcat aggttttttc
     7381 ttagatcagg agcaccgtgt ataaagaaaa attcgcttcg tttttgactg aaataaaaaa
     7441 gtgtctaatt ttcatgaaaa tgttttgaat tttctgtttc atggtggtat gataccgtgt
     7501 ggttagaaac cgattggaaa aaaccagata cgcacatgaa ttgattttat gtgaatttcg
     7561 gggaaaagag gtgaaatgga ttggatgaac gctcgctaac gttccacatt ggaccaattt
     7621 ggtttgatgg tacagtttgt ataatggtgt tactgacctg tttgattgtt ttcttcttag
     7681 cttacttttt tacaagaaat cttaagttga aaccaacagg caaacaaaat gcgatagaat
     7741 gggtcattga ttttagtcga ggaatcgtaa cagataatct gcctagaaaa gaattgagca
     7801 atttccattt gttggcattt acattgtttt tatttgtttt tgttgcaaac aacatcggtt
     7861 tagtaacaaa aatcgtttta cctaacgaaa caacactttg gaaaagcccg acagcagacc
     7921 cgtttgttac tttaactttg gcatttataa tgatcttact tactcactta ttcggggtga
     7981 aaaagttagg cttcaaagga tatttcgtaa attcgttctt gaaaccctat agttttatgt
     8041 ttccgatgaa actgattgaa gaatttacaa acttattaac acttgcttta cgtctttatg
     8101 ggaatattta tgcgggggaa gttttgctaa ccttgattgc aaaaatgatg acaagcttag
     8161 gctggttctc attaccatta gcaattccat tagagatggt ctggatcgcg ttctccttgt
     8221 ttattgggag catccaagca tttgtctttg tcactttgtc aatggtttat atgagccata
     8281 agattgaagt agaagaataa tactcggtta aaaaacaaac tactatttat ctattttagg
     8341 aggaaaacaa ttatgaatta tatcgcagca gcaatcgcaa tcatgggagc agcaatcgga
     8401 gccggttatg gtaacggtca agttatctct aaaacaatcg aatcaatggc tcgccaacct
     8461 gaaatgtctg gtcaattccg tacaacaatg tttatcgggg tagctctagt tgaagctgtt
     8521 cctatcttag gggtagttat cgccttgatt ctagtttttg ccgtttaatt gaatcagtat
     8581 taaggcagaa acaacatcgt ttgttactgc ttttttacat ggaaactaga aatactccaa
     8641 agaaaggaag gctcgcctat gctgaatcaa ttggcaattg cagaagttgg caatgcgatg
     8701 ttaggtaata tcatcgttgt cagcggctca tttttgatac tactggctct cttgaagcac
     8761 tttgcttggg gaccaatcag cgatattttg aaaaaacgtg aagacaagat cgccaatgat
     8821 ttagattctg cagaacaatc tcgcatcaac tcagcgaaaa tggaacaaga acgcgaacaa
     8881 caattgttag cctctcgttc tgatgcagct gatatcatca aaaatgcgaa agaaagtgga
     8941 gaattaagcc gccaaaatat tttgaaggat gctcaagaag aagcagctcg tctaaaaagc
     9001 aaagcccaag ctgatatcac tgtagaacgt gattcagcgc tgaactctgt aaaagacgac
     9061 gttgcagaac tctctcttca aatcgcggaa aaaatcttga acaaagaatt atctccagaa
     9121 atgcatgaat cattaatcaa tcaatacatt gaaggtctag gttcttcaaa tgaaactaga
     9181 taaatataca gtaggaagac gttacgggaa agctttattt gaactggcga tcgattcaaa
     9241 tcaagcagaa gaaatctatc aagatctcgt aagtttacgt caaatctatg aacaagtacc
     9301 tggattaggg aatatgttaa gtgatgttcg tctagaacct catgaaaaac gagaaatcat
     9361 ggacaagctt gtcagcggtt atgatgggat cgttaaaaac tttttagaag ttgtttatga
     9421 gtataaccga atggatgacc ttttgttcat gatcgaagaa tatgaacgtc gatatgatga
     9481 acatcaaggg ttactattgg gaagtgtcac aacagcagtc ccactttcag acgaacaacg
     9541 aatacaatta gaaaagaatg ttgcaaaaac aatggactat caatcagttg aattaaaaca
     9601 aatcgttgat tcttctatta ttggtggcgc gattgttgaa gccaaccatc gagtaatcga
     9661 cggaagcatt cgcacgcaat tagaaaaaat gcgtaatcaa ttaaacagat agtacaaaga
     9721 ggtaggtgaa tcgaatggcc atcaaagcag aagaaattag tgccttgatt aaagaacaaa
     9781 ttgaaaatta tcaaaacgtg ctttcagtcg aagaaatcgg taccgtaact tatgtaggtg
     9841 acgggatcgc tcgtgcacat ggtttagaaa acgcgatgag tggtgagttg cttgaatttt
     9901 caaacggctc atacggaatg gcgcaaaact tagagtcaaa tgatgtaggt atcattatcc
     9961 ttggcgattt cgagtccatt cgtgaaggag ataaagtaaa acgaacaggt aagatcatgg
    10021 aagttccagt tggagaggcc ttgatcggtc gggtagtcaa tccgctaggt caaccaatcg
    10081 acggactagg tgaaatcgtt actgacaaag ctcgtccagt agaagcgatg gcaccaggtg
    10141 ttatgcaacg taaatctgtt aacgaaccaa tgcaaacagg gctaaaagcg atcgacgccc
    10201 ttgtgccaat cggacgcgga caacgtgaat tagtcatcgg tgaccgtaaa acagggaaaa
    10261 cttctattgc gatcgatacg atcatcaacc aaaaaggtca agatatgatc tgtatctatg
    10321 tagcaatcgg acaaaaagat tctacagttc gtacacaagt tgaaacattg aaaaaatatg
    10381 gcgcaatgga ttacacaatc gttgtgaatg ccggtgcgtc tcaaccagca ccattgcttt
    10441 atatcgcacc atatgctggt actgcaatgg gtgaagaatt catgtacaac ggtaaacatg
    10501 tattgatcat ttttgatgat ctttcaaaac aagctgttgc ttatcgtgaa ctttccttgc
    10561 tacttcgccg tccaccaggt cgtgaagctt atcctgggga tgttttctac ttgcactcac
    10621 gtttattaga acgtgcagca aaattaagtg atgatttagg tggcggttca atgactgcct
    10681 taccatttgt tgaaacacaa gctggagata tctctgctta tatcccaaca aacgttattt
    10741 cgatcactga tggacaaatc ttcttagaaa gcgacttgtt ctacgcaggt actcgtccag
    10801 cggttgatgc cggactatct gtatctcgtg taggaggttc tgcgcaaatc aaagcaatga
    10861 aaaaagttgc tgggacttta cgcctagact tagcaagtta ccgtgaatta gaagcattta
    10921 cacaattcgg ttctgacttg gatgcagcaa cacaagcaaa actaaatcgt ggacgccgta
    10981 cagtagaaat cttgaaacaa aaattgcatg caccgctagc tgttgaaaaa caagtagtga
    11041 ttttgtatgc tttgacacat ggattcttag atagtatccc tgtagacagt attttagact
    11101 tcgaacatga attattcgaa tatctggata ccaatcattc agatatcttt gaaacaatcc
    11161 gtacaacaaa agatcttcca gaagaagaag cgttgaacag tgcgattcag gaatataaag
    11221 acatgttctt agctacaaaa ggaagtaatt cctctacaga agataaattg aaatccattc
    11281 aaaatgcata gtgaggtgag atgaatgggt gcttcattaa atgaaatcaa gacacgtatc
    11341 gcttcaacga aaaaaacgag ccaaatcacg cgggcaatgc agatggtgtc tgcttctaag
    11401 ttgacaaaat ctgaagcttc ttcacagaag tttcaggttt atgcaacaaa agtaagagaa
    11461 atcgttacgc atttgactgc tactcagtta aatgatattg cctcagaaaa tcctagaggg
    11521 gatatcaatt acaacagcat gctgctcagc cgtcctgtga agaaaactgg ttatatcgtc
    11581 atcactgcag atggcggttt agtcggtgga tataacagct cgatattgaa acaaacgatg
    11641 tctattttag aagaagacca ccaatcaccc gatgattacg tgatgatcgc aattggcgga
    11701 actggtgcag acttctttaa agcacgcggt atcaatctag catatgagct gcgcaatcta
    11761 tctgatcagc caagctttga tgaagtccgc aaaattgtaa atatggcaac aactatgttc
    11821 caaaacgaag tattcgatga attatacgta tgttataacc atcatatcaa ttcgctaact
    11881 agtcagtttc gtgtagaaaa aatgctgcca atctcagact tagatccaga agaagcaacg
    11941 actttcgaac aagaatatat ttttgaacct tcaaaagaag aaatcttgtc tcagttattg
    12001 cctcagtatg ctgaaagttt gatctacggt gcgatcgttg acgccaaaac agcagaacat
    12061 gctgctggta tgacagcaat gaaaacagca actgataatg ctgctaatat cattgatgag
    12121 ctgacggttt cttataaccg tgcaagacaa ggggctatta cccaagaaat taccgaaatt
    12181 gttgccggag cttcggcact agaatagtat tgagattgga ggaaaaaaga tgagttcagg
    12241 caagattgtt caagtaatcg gtcccgttgt cgacgtggaa ttttctttag accaatcctt
    12301 accagatatc aacaacgcat tagttgttta taaaaatgac gaaaataaat caaaagttgt
    12361 tcttgaagca gcgttagaat taggggacgg agtgatccgg accattgcga tggaatcaac
    12421 agatggttta caaagaggaa tggaagtcat tgatacaggc aaagcaatct ctgttcctgt
    12481 aggtaaagaa acattaggtc gcgtattcaa cgtactagga gatacgatcg atttagaaac
    12541 acctttccca gaagatgcgg aaagaagcga aattcataaa aaagcaccag cctttgacga
    12601 attaagtaca agtacagaaa ttttggaaac agggatcaaa gttatcgatt tgcttgcccc
    12661 atatttaaaa ggtgggaaag tcggactatt cggtggtgcc ggtgttggta aaaccgtact
    12721 gatccaagaa ctgatccata atatcgccca agaacatggt ggtatttctg tatttaccgg
    12781 tgtaggtgaa cgtactcgtg aaggtaatga cttgtattat gaaatgaaag attcaggagt
    12841 tatcgaaaaa acagccatgg tgttcggaca aatgaacgaa ccaccaggtg cacgtatgcg
    12901 tgttgctttg actggtttga cgattgcgga atatttccgt gatgtagaag gtcaagatgt
    12961 actgttgttt atcgacaaca ttttccgttt cactcaagct ggatctgaag tatcagcctt
    13021 gttaggacgg atgccttctg cggttggtta tcaaccaaca ttggcaacag aaatgggtca
    13081 attgcaagaa cgtatcacat ctacgaaaaa aggttcgatc acatcgatcc aagcaatcta
    13141 tgtgccagcc gatgactaca ctgacccggc gccggcaaca gcattcgccc atttggatgc
    13201 gacaaccaac ttggaacgta aattaacaga acaagggatt tatccagcgg tagatccatt
    13261 ggcttcttct tcaagtgctt tagcacctga aatcgtggga gaagaacatt ataaagtagc
    13321 aacagaagtc caacacgtat tgcaacgata tcgtgaatta caagatatca ttgctatctt
    13381 aggtatggat gaattatccg atcaagaaaa agtgttagtt tctcgtgccc gcagagtcca
    13441 attcttcttg tcacaaaact ttaacgtggc tgaacaattt actggtcttc caggttcata
    13501 tgtccctgta gaagaaacag tgaaaggatt ccgcgaaatc ttggaaggca aatacgatga
    13561 tcttcctgaa gaagctttcc gaagtgttgg ccgaatcgaa gacgtcgtag aaaaagcgaa
    13621 aacactaggc tactagaaag gggtgcccca tggatcaata tttaacagta aatgtggtaa
    13681 ctcctgccgg tttggtctat gatcaccatg cagctatcgt cgttgcacgt acgacagctg
    13741 gtgagcttgg gattttgcct aaacatgcac caatcatcgt tccattgacg attgatgaag
    13801 ttcgtgtaaa acgaacagat tcagataccc acgtcgattg gatcgctgtt aatggcggaa
    13861 tcatggaagt tcgtgataat gttgtttcaa tcgttgctga tagtgcggaa cgtgaacgtg
    13921 atatcgatgt atctcgtgcg gaacgtgcaa aacaacgcgc agaacgccaa atcgaagaag
    13981 caaaagcaaa acaagatacg aacgaattga aacgggcaac tgttgcgttg caccgagcaa
    14041 tcaatcgtat caaagtatct aagcactcat aaaatgagat gcggatgttt atccgtgttt
    14101 aagaacagta aaaagaggtt atgacagaag tggtcagctt caagacataa gaaggaattt
    14161 acgaaaattg cttctcaaat ttttgtgaaa tctcagctta tttttcgaag aagctacttc
    14221 tggaacaccg tatatcagag gttgtgaaat cagcgttttg tcctgagtat tagagaaaca
    14281 aatggaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagatgat
    14341 tgaacaccgt ttatttggaa taagggactg cgacaagact tttgtctcag tccctttttt
    14401 tgcatatttt tagcatttat ttcctaaaaa atgtaagggg attcctatat ttttaagtat
    14461 acttgttttt ccttttatac attttatggt attatcattt tattagttat tataaaacaa
    14521 tcgttagaaa ggacgtcatt atgcaagtat acggaattga tgcggtgctt cgaatcgtga
    14581 gtcatttggc gtttatctac ttagccttct ggtctttacg ttcgttacgc attgaaactt
    14641 ttttcagacc aatgcataat acgcaaatta gaatggcaat cgtacttttt tcaatttttt
    14701 tagggtacac tgcaagtaat tttttcttag aggttatcgc tttatgccga aatcttttcg
    14761 ttagcctgta aattttaaaa aaaggtacta ttttaaaagc aaggatgtta taatagaaaa
    14821 gattttgagt aagtgtatgc tcattggaat tttttggagg gaacaacatg gaagagatca
    14881 tcgtaagagg tggcaatcaa ttaaatggaa cagtaagaat tgaaggagct aaaaatgctg
    14941 ttttgcctat tttagctgct agtttgttag cagaagaagg tattacgaca ttagacaacg
    15001 ttccgattct ttctgatgtc ttcactatga atcaagtgat tcgtcactta aacgtagatg
    15061 tagattttga tgaacaaaag aatcaagtaa caatcgacgc ttcaagacaa ttagaaatcg
    15121 aagcgcctta tgagtatgtt agtcaaatgc gtgcttcaat cgttgtgatg ggtccgctac
    15181 ttgccagaaa tggacatgca aaagtagcta tgcctggtgg atgtgcgatt ggtaaacgac
    15241 caatcgattt gcatttaaaa ggatttcaag ctttaggagc aaaaattatt caaaaaaacg
    15301 gctatatcga agcaattgcg gatgaattga tcggtaacac gatttattta gatttcccta
    15361 gtgtaggtgc tactcaaaat atcatgatgg ctgccgtaaa agcaaaagga acaaccatca
    15421 ttgaaaacgt agctcgcgaa ccagaaatcg ttgatttagc aaacattttg aataaaatgg
    15481 gcgctcaagt ctatggagct ggtactgaaa caatgcgtat tgaaggcgtg gatcatttac
    15541 atgcagtcaa ccactcaatc gtacaagatc gtatcgaagc aggtaccttt atggttgctg
    15601 ctgcaatgac acaaggcaat gtgctgattg cagatgctat ctctgaacat aaccgcccat
    15661 tgatttctaa attgatcgaa atgggtgcag aaatcattga agaagaaggc ggagtccgtg
    15721 tcatcggacc aaaacatatc ttacctacag atgtaaaaac gatgcctcat cctggtttcc
    15781 ctacagatat gcaagctcaa atgacagcta tccaattagt tgcagaaggt acaagtgtgg
    15841 tgactgaaac agtgtttgaa aatcgtttcc aacatttaga agagatgcgc cgtatgaacg
    15901 ctcatgtcaa aatcgacgga aatgtagcaa tcatggatgg aaaccatgaa ttgcaaggtg
    15961 cagaagtata tgcaacagac ttgcgggcag cagcagcatt agttttagct ggcttgaaag
    16021 caaatggaat cacacgtgtg cgtaacttga attatctaga ccgtggatat tataatttcc
    16081 atatcaaact acagcaatta ggtgcagacg tcgaacgtgt agacatggat caaacttctg
    16141 ctgaaaaaac agcacaaacg attgcttaag gaagtggctg aatgagtaac caacgatata
    16201 tcttattaac tttgataaag attttagtgg tgatcttatt gcttatcctt ctttttgtgg
    16261 ccggaacgat gatcggatat ggtgtcatcg gtggcggcaa tccatttaaa gtattccagc
    16321 caagtttgtg gattcatatt cgagactttt ttcattgata aataagaatc acagtggttc
    16381 tagtcgatat gaagtgagcc atgaagcaga acatgataaa agaaaacaga agggaacgtt
    16441 gacgttagtc gcgatccctt tttttagtaa aagaaaagaa ggaatctaat tgatgaagaa
    16501 gatattgatt tatttagtta gaggatatca gcgctttatt tcgcctgcat ttccacctag
    16561 ctgccgttat tacccaacct gttccaatta tatgataaaa gcaattcaaa cccatggtgc
    16621 gctaaaaggg acggtaatgg ggactgcacg gatcttacgc tgtcatcctt ttgtcaaagg
    16681 cggtatcgat tatgtaccat tgacattccg attaacgcga aataagaaag ataaaaaaga
    16741 accagattat aaaaaggttg tgaaaaatac gggtagcact gagcgataaa gaaaaagagg
    16801 aaatttattg tcaaagtaac aactccgcaa ctacttatat gggggacaaa aaatgatcga
    16861 agaactaaac aagaaagatt taccatggtc cttgttatta gatgcagatc ctgataaaga
    16921 aaaagttcaa gtatatgttt ccagagggag tggctggata tggaaagaaa aagaaaaaac
    16981 aataggtgta ctgatctatg tggcaaggga aaaagaatac gaaatcgtca atgtagcagt
    17041 aaccccaacg tatcaaggaa aggggattgg cggaaaatta cttgagacag cttttcagaa
    17101 gctttcacag ctagcttcaa accaaactcg gatcattata cgtactggca ctactagtag
    17161 cgctgcgtta cacctctatc aaaaaatggg ctatgtggag attggtcgag tcaaagacta
    17221 ttttatccat cattactctg agcccatttt tgaaaatgga gatcaattaa gggatcaagt
    17281 gattttagca attcacttac ctaaaaatag ctttaactaa taaactttca attcgagaaa
    17341 atgctttcaa atgctaaata gatacattac aatagcttta gcaaacaaaa aggagaatat
    17401 ttttatggga atattagttt ttgattttgg tggatcggct gtcaaatatg gttgttggga
    17461 tggcaaagag attaaaggta agggacaatt tgcaacgcct gaatcttggg aagaaatgaa
    17521 aacgcaatta tttcaagttt ataaaaaaat actcgtttct tttgaagtcg aaggaattgg
    17581 aatcagctct cctggagtag tgaataatga gaaacaggtg attgagggaa tcagtgcgat
    17641 tccttatatt catggattta atattttccg agatttggaa actctgtttc aactcccagt
    17701 aacgattgaa aatgacgcca actgtgccgg aatggctgaa ttttatgaag gtgctgcccg
    17761 tgattatcaa gatgttgctt ttgttgtggc aggaactggg attggcgggg cgttgttcac
    17821 gaaaggacaa ataaataaag gggcacatct ttacggtgga gaatttgggt tgatgttctt
    17881 agatggcgat aagacattca gtaaacttgg tacagcggta cagatggctt ggcgttattg
    17941 tgaacgcaaa ggtctcgata aaaatgctta taccggaaaa gatgtctttg aattagcaga
    18001 aactggagat gcaatagcaa aagaagaagt tgagagtttt tatacgtatc ttacaaaggg
    18061 attattcagt attcaatttt cgtttgatcc agaagtgatc gtattaggcg gcggggtttc
    18121 tgcaaaagaa gggctcattg atgagatcaa aagccgaatg aagaagctga cagaacaatt
    18181 cgatctgcat gatttcgaac cgcagatttt attatgtgag tacagaaacg atgctaatct
    18241 tataggtgca gcagcaaact atctcgctgt acgtcaagga gagaattaag agctttagag
    18301 aaggtgcagc ttgcataaaa gaagagaatg aaatgaatag gagattttta cctaaggaat
    18361 gttttatttg ctgcagtatg ataaaatatc tgagaggtga aaatgatgtc aaaaaaaaat
    18421 aaagatattg aagtgcgtat agaagaagtg aaaaaagata tcaaaggtaa gaattatgaa
    18481 gtcactcagt tatttatagg gaaaaaaatg attggtgaga tcttagccta tgggccaaaa
    18541 gaatatgaaa ttttctttgg tgaagaagat tttggaaaag aaaaaagtct tgaaaatgcg
    18601 atagaaacag tgattcgaca ctggaatctg cacgaataaa aaaatatcaa aaaaactttc
    18661 aataaagggt tgcaaaaatc tggaaaatta ggtattataa ctaagtcagg ttgatacaaa
    18721 cctttcggag gagtagcgaa gtggctaaac gcgacggact gtaaatccgt tccttagggt
    18781 tcagtggttc gaatccactc tcctccattt cattggggta tagccaagcg gtaaggcaag
    18841 ggactttgac tccctcatgc gttggttcga atccagctac cccagtatac cacaatcgta
    18901 agctctcgtt agttcgagag ctcttttttt ttgctttgct accttgttcc ttgaagattc
    18961 tgctgaatta agatagagtt aagttaacta caagaattgg agggatccac atgaaatgga
    19021 aagaaaaagt agacgaagca gctgaaaagt tatatgattt ggtcaaatca gaaaaataca
    19081 atattgaagt gaatattccg aaaaaaggcg gaaaagcggt ccaggtaaaa tcaaaacgcc
    19141 caacgaatca tacaaaaaaa tggatggcta aaaaccgcta ataataacaa aagatccata
    19201 agtcggcatt ttcatcgatt ttagggtctt ttgttatttt ctaggttgaa catatctaaa
    19261 aaattgtttc tgacaatagg agtagggaga acgatgtgtg tctaaatcct ctgttgatgg
    19321 atagaaacct ttatcttttt cagttgtgaa tcaggtatat accagttata atagaagaaa
    19381 cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt atatatacag attcatgacc
    19441 aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg agaccgtctt ccttcagaaa
    19501 gagaattagc agtgaaattc aatgtcagcc gtatgacgtt gagacaagca attcaaaact
    19561 tggcagatga aggaatctta gaaagaaaga tcggttcggg tacgtatgtt gcgcgagaaa
    19621 aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga aattatggaa tcacaaaatc
    19681 gagtaccgtc tagtcggaca atctcttatt tcttgacttc gccaagctca agtgaaatgg
    19741 aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat ggaacgtatt cgtttcgcag
    19801 atgatgtccc tatttgtttt gaagttgcca gtgttccaca aaagctgatc gatggctaca
    19861 gcaaagcaga gattactgaa tcgttgtatc gcacgctaga agaaaaaggc gggaaaaaga
    19921 tcggtccagc gaatcaaacg attactgctg tgttggcttc tgagaaaatt gctgactatt
    19981 tagaggtaaa acgaagagat gcagttcttc gtttgcgcca gatttcttat tttgaagacg
    20041 gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa tcgttttgag ttttatttgg
    20101 aaaaataaaa aacaggtgat ccctgattcc ttattagaaa taaaagagaa tcagaatcac
    20161 ctgtttttat atatgatcag ttatcagttg ctcgtgataa gaaacagaac cctcttcaaa
    20221 ttggacttgt ccgttcagca gctcaatgat ggattcttta aagtggttag cacttttttc
    20281 atctacagca cagatgacgc taacttgatc agtatattgg atttctttta taaaaaaatg
    20341 ttggagttca agataattct gtaattttcc tagttgcgga tacgtgatca tcacagagat
    20401 ctcctgttgc aaggtacctt ctacgattcc aatgtcagac aatgcttgag agaccgcatg
    20461 agtgtatgct cgtattaatc cgcctgtgcc gagtttcgtt cctccaaaat atcgagtgac
    20521 tactgcagct acattgatca gttgattttt tttgaggact tccaacatcg gtacgcccgc
    20581 cgttccactt ggttctccat catcgctgct tcgctggatt tcattttttt ctccaatgat
    20641 gaaggcgctg cagttatggt tcgctttcca atgttctttt tttatttctt gaatgaaatt
    20701 ttttgcttgg tcctctgatt ctattcgtct aagagaacag ataaagcgtg attttttgat
    20761 ctcaatctca ctttgtccat tttcttttat tgtacgatag cgctcgatca tcttgtcacc
    20821 tctttttttc attttcttta ttcagtataa ataaagttct aggttgtttc aacactgtaa
    20881 cggttgcgca attgctgtag tatgcgataa taaagacaaa ggcaggtgga tctggggatg
    20941 caggaacgaa tgaaaaagta cgatgcaatt acgcattatt tgaagaacaa tggcggctct
    21001 caagtaacgt tgacctttac ccaatttgac gaactgctgt ttcccagtaa cggacttccc
    21061 aaaacagcaa gggaaagtac agattggtgg gcaaatgatt ataaacaccc tgaaaaaggt
    21121 gcctatggct ggataaatgc aggctatgaa gtagttgtca tcaatttgga caaagaatat
    21181 gtcgtgttca ataaattagt gaaatctagt tggctatttg attaaataaa ccgcgtttag
    21241 aaaacgttga caaatttctt tctttttata taataaagta acagacgcga agaaaacgtt
    21301 tgcgtagttt gttttaaaaa ggagagaaaa atatggctaa aaaaacaatc atgcttgtat
    21361 gttcggctgg tatgagcaca agtttactag taacaaaaat gcaaaaagct gctgaagcaa
    21421 aaggaatcga ctctgatatt ttcgctgtgt ccgcttctga cgcagataac aatcttgcaa
    21481 ataaagacgt ggatgtgttg ttattaggcc cacaagttcg ctttatgaaa gctgattttg
    21541 agaaacgtct ggaaccaaaa ggcattcctt tagatgtcat caatatggct gattatggta
    21601 tgatgaatgg cgaaaaagtg ttagatcaag caatcgcgct cataggtgaa taaatagaac
    21661 agtataacta tcgaacaagc gatccaaaaa atagatcgct tgttttttcg tatatttttt
    21721 tagggcgaat aatatgaatc atttttgggg tagaaggctt ctgacgagag atattaaagc
    21781 aatagactgt gaggaggtaa atttaccaaa ttatgagaag atcgcagcag tggaacgttt
    21841 gggcaactgg attctttgtc atcgctgtgg aatgaggacg tccgtcattg ttggacagct
    21901 tgcagattac ggttattttt gtacacattg tctatattta gggtgatgcg acactcaaca
    21961 agatttatat ttgtttgatc agcctgaagc aaaacctcga gaagtcgtct ttttttggac
    22021 aggacagctt actgaaaaac aaacggaaat cgcaaagaga atactttgcc attcagaaaa
    22081 aagacatcat cttatttggg ctgtaacagg tgcagggaaa acagagatgc tttatcctat
    22141 tttagtggaa actttaaaag ctggaggaag agtagcgatt tgtacaccgc gagtcgatgt
    22201 ttgtaatgaa ttgtttttac gctatcgtta tgtttttcca gaagaaaaaa tcacgttgct
    22261 ccatgggaat acagcaacgg cttatacctt tacttctttt gttatctgta cgattcatca
    22321 gctataccgt ttttatcggt cctttgattt aatagtgatc gatgagatcg atgcgtttcc
    22381 ttataacggg gatgctgggc tagctcacgc tgtgcaaaca gcaatcagat caaacggccg
    22441 attcatctac ttaagtgcca cgcctgatga gaagttacta aaggaaatca agcagacatt
    22501 tgagctccac aaacttgcct tgcgctttca tcggcgcttg cttcctgaac ccgtgcttgt
    22561 tttcatgaat aattggcgac aaaaatgtgg aaacaaaaag aaaatcaact ctttggtgcg
    22621 gatcattcag caactaatca aagaaaaccg tatcctttta ttttgtccaa gtattacgat
    22681 gatgcatcgt ttaaaaatca cactgcaaga agcttttcca gaatatgaga taacagatgt
    22741 ttcttcacaa gacgatcaac gggcaaagaa agttcagaaa atgcgagaag caatatacga
    22801 gatactgtta accacaatga tcctagaaag aggagtcaca tttgaaagaa tctctgtcat
    22861 cgttttagga gcagatcatc cagtttttac taaatcctct ttggtgcaaa tcgcaggaag
    22921 agctgatcgt aaaggtcctt atacgaacag tcgagtctat tttttctatg aagaaaaaac
    22981 ttcagctatt cgaaaagcct gtcaggaaat caaagaaatg aacgaagaag ggaagcagat
    23041 gctgtgagat gtagttgctg cggtaaagac atcattcaaa atttaacatt cagcgagatc
    23101 atcggagtgt cgaaatctag acttcctcag acatgttcag tttgccgcgc acaattccat
    23161 ttattagaag caaaaggttg tgcaggttgt caaaaaccaa tccatttgaa atacgaggag
    23221 acgaaaaaga aagcaaatac tagtttcgtt tactgcaacg actgtcaaga atggaagaag
    23281 aaatatcctg catatgattt tcaacaccgc gcctttttta cgtacgatga aaagatgcag
    23341 gcttggatca atcggtacaa gtttatggga gatatccgat tagcagggac ttttgcatat
    23401 aaatggactc agataaaaaa aagattaccc ggcatatatt tattgtccaa tacctttatc
    23461 tgaaaaaagg tggctagaaa gaggattcaa tcaagtaagc gagatgttga aagcagcaaa
    23521 aattcctttt cagatgctgc tcaaaagaga gcaacatctg cttgctcaag caaaaaacac
    23581 gcgaaaggaa agattggaga tgcctcagcc ttttgagctt gccgtacctc tttctaagat
    23641 acaacataaa aaa
//
LOCUS       DADVXU010000037        23622 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.039, whole genome shotgun sequence.
ACCESSION   DADVXU010000037 DADVXU010000000
VERSION     DADVXU010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 23622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.039"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            208..1014
                     /gene="proB"
                     /locus_tag="KB953_001368"
     CDS             208..1014
                     /gene="proB"
                     /locus_tag="KB953_001368"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HBB6866353.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            1011..2258
                     /locus_tag="KB953_001369"
     CDS             1011..2258
                     /locus_tag="KB953_001369"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HBB6866354.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            2334..2678
                     /locus_tag="KB953_001370"
     CDS             2334..2678
                     /locus_tag="KB953_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866355.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(2795..3121)
                     /locus_tag="KB953_001371"
     CDS             complement(2795..3121)
                     /locus_tag="KB953_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866356.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3736..4938)
                     /locus_tag="KB953_001372"
     CDS             complement(3736..4938)
                     /locus_tag="KB953_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB6866357.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            5055..5570
                     /locus_tag="KB953_001373"
     CDS             5055..5570
                     /locus_tag="KB953_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBB6866358.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5769..6215)
                     /locus_tag="KB953_001374"
     CDS             complement(5769..6215)
                     /locus_tag="KB953_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HBB6866359.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6262..7740)
                     /gene="msr(C)"
                     /locus_tag="KB953_001375"
     CDS             complement(6262..7740)
                     /gene="msr(C)"
                     /locus_tag="KB953_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HBB6866360.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(8094..8723)
                     /locus_tag="KB953_001376"
     CDS             complement(8094..8723)
                     /locus_tag="KB953_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBB6866361.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(8707..9171)
                     /locus_tag="KB953_001377"
     CDS             complement(8707..9171)
                     /locus_tag="KB953_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBB6866362.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(9658..10926)
                     /locus_tag="KB953_001378"
     CDS             complement(9658..10926)
                     /locus_tag="KB953_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBB6866363.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(11157..11894)
                     /locus_tag="KB953_001379"
     CDS             complement(11157..11894)
                     /locus_tag="KB953_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBB6866364.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(11961..12530)
                     /locus_tag="KB953_001380"
     CDS             complement(11961..12530)
                     /locus_tag="KB953_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBB6866365.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12658..13530
                     /locus_tag="KB953_001381"
     CDS             12658..13530
                     /locus_tag="KB953_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBB6866366.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            13872..14744
                     /locus_tag="KB953_001382"
     CDS             13872..14744
                     /locus_tag="KB953_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBB6866367.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            14741..16699
                     /locus_tag="KB953_001383"
     CDS             14741..16699
                     /locus_tag="KB953_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBB6866368.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(17031..17882)
                     /locus_tag="KB953_001384"
     CDS             complement(17031..17882)
                     /locus_tag="KB953_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBB6866369.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            18005..18472
                     /locus_tag="KB953_001385"
     CDS             18005..18472
                     /locus_tag="KB953_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBB6866370.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(18735..19217)
                     /locus_tag="KB953_001386"
     CDS             complement(18735..19217)
                     /locus_tag="KB953_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBB6866371.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(19317..20180)
                     /locus_tag="KB953_001387"
     CDS             complement(19317..20180)
                     /locus_tag="KB953_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBB6866372.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            20778..21653
                     /locus_tag="KB953_001388"
     CDS             20778..21653
                     /locus_tag="KB953_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866373.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(21798..21950)
                     /locus_tag="KB953_001389"
                     /pseudo
     CDS             complement(21798..21950)
                     /locus_tag="KB953_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(22218..23549)
                     /locus_tag="KB953_001390"
     CDS             complement(22218..23549)
                     /locus_tag="KB953_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBB6866374.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
BASE COUNT         7266 a         4780 c         4191 g         7385 t
ORIGIN      
        1 tactcctatt ctaacatatc cgaggctgta aaagactttc tttacaaatt cttcaagaaa
       61 ttctgctatc ttaaagtaaa ccattaattc gttctttttc caaacgactg caagttgtac
      121 aaaaaaacta ttttttacat gactttactc gtcaaaacaa gtagaataga acgaaattaa
      181 tgagtggata tttgattgag gtgtcacatg cgtaaagaaa ttaaaaatgc aaaacgaatt
      241 attgtcaaag taggaacaag tacattgatt tatcccaatg gaaatatcaa tcttgcggca
      301 atcgatcagt tagcttttgt cctttctgat cttagaaatc ggggaaagga agtgatctta
      361 gtctcatcag gagctgtagg cgttggtatg cataaattac atttgtcaga acgtccgact
      421 ttgattcctg aacagcaagc tgcggcggct gttggtcaag ccgaactgat gaatatctat
      481 aatcaaagat tcagtgtata taatcaacag acggcacaga tcctcgtgac aagagacgtg
      541 gtagattatc ctgaaagtcg taaaaatgtg aggaatacat tggaacagct tttgattatg
      601 gacacgatcc ctatcgtgaa cgaaaacgat actgtagcag tggatgaatt agatcatctg
      661 acaaaatttg gcgataatga tcagctgtca gccatcatgg caaaacttgt cgcggcagac
      721 cttttgatcg tcctttctga tatcgacggg ttttattcag ataatccgac tacaaatccc
      781 gaggctcaga tgtatcgcta tatcgataaa atcgatgaaa agattctggc acaagcaggt
      841 ggtgctggta gcacatttgg aacaggcggt atgcagagta aattaaaagc cgcttctagg
      901 atatttgaag caaacagcgc aatgatttta gcaaatggaa aaaatccggc aattattttt
      961 gatattcttg caggaaaaga agtaggtact ttatttaagg aggacttgaa atgactgact
     1021 tgatgcaatt aggacaacag gcaaaagata gcgcgcaaca actagcactt atgcctacaa
     1081 accgcaaaaa tgagctactt gttcaaatgg cacaaaccat caaaaagaat caacaagcga
     1141 tcattgaagc aaatcataaa gacttagaaa aagcagtcga aaacaatatc agtgaaacga
     1201 tgcaagaccg cttgcagctg acacctgagc gaatcaatgc catggctaca gagatcgaaa
     1261 aaattgcttc gttagacgat cctattggaa aagtagatga gatgtggacg aacaccgacg
     1321 gattgagaat cggcaaaaag cgcgtgccac taggcgtgat cggcatcatt tacgaatctc
     1381 gtccaaatgt cacgacagat gcagctagtc tagcattcaa atcagggaat gccgttatcc
     1441 ttcgcggtgg caaagaagca ttcttctcga atcaattatt agtccaatta ttgcagcaag
     1501 tactggtcag tgctggcgaa tctccttatg ccatccagtt tgtcgatgat acttctcatg
     1561 aaactgcagg acagttgatg cgattgacag aatacttaga tgtgctgatt cctcgaggcg
     1621 gagcaaaatt aatccaacga gtcaaagaac aggcaacggt acctatcatt gaaacaggta
     1681 caggaaataa tcacgtatat atcgataaag aagcacagtt atctatggca gtgaacatca
     1741 tcgtcaatgc aaaagcttcc cgtccatctg tctgtaacgc ggcagaaaca ttgctgatcc
     1801 attctgagat cgcacctttt ttcttgccag ctattgaaaa agaactggta gaacacgggg
     1861 tctctttacg tgcggatgct cgtgcgttag aatatctaga gacggctgct ttagcagaag
     1921 acacagattg ggatactgaa tatctagatt atatccttgc tgtcaaagtg gtcgattctt
     1981 tggatgaagc aattgcacat atcaatcggc ataatactaa acattcagaa acgatcgtga
     2041 cagatagtta tgcggctagt caacgtttct taaatgaagt ggacgcggcg gctgtctatg
     2101 tcaatgcctc cactcgtttt acagacggat cggtatttgg ttttggtgca gagattggta
     2161 tctctactca aaagttgcat gcccgtggtc caatggggtt aaatgaatta acttccacaa
     2221 aatatatcat ttatggcgat ggacaaatcc gcgaataatg tttcgtgtga aacattattc
     2281 ccgccttttg ccttttttct gtaaaggttc tatccaacta gaaaggtgtc aatatgtttg
     2341 aaattttaat gatttttttt atttacctta tttctctgaa tatcgctgct tttctgggag
     2401 tcggtatttt atcccttttc tttcaattca aaaaacgaag catagggagt caacgggaaa
     2461 aatggtcgca gtattttgac aaaatcgggc aaaaagggct ggttacacgc ttgcacatta
     2521 gttacatggt tgcattatgc ttattagcga caatcaatta ctattctttt ttcgatcatt
     2581 caatcgctta tacgattacg ctactgattg ctgggatttt tcacttatct tataaatatc
     2641 agctgaataa aaaccacttg aatcggactt tccgctaaaa aatatccgta cgataagtag
     2701 agtgccttga agatcggcaa aaaactgccg gactttttag aggctgtgac aaagattttg
     2761 tcacagcctc tttttccata taaacggcgt tcgcctacca gctgcctggt aaatctgttt
     2821 cctcttcgtt aagaagcgaa aatccgtcaa atgtttcgcc atcataataa cgaagcagct
     2881 catccatgga caagttcatc gttttttctt tttcttcttc cgctgattca gccagtagct
     2941 gttcccgata cacctgatac ataccagcta ttttttgctg catcgctttt ctagtttcac
     3001 cttccgcatt gattattggc aattctttga tatagccacg ataaatcaac cgatcatctg
     3061 aaaattcaat gggtaattca acgagtgtca acccgacttc ctgaaaatat tcttccagca
     3121 tctattatcc ccccattctt tttcttaaat gaattttagc ttacctactg aaaattttcc
     3181 agtttcatta ttttgagaaa aaaagggaat gtgacaaaaa tcgtgtcaca tccccttttt
     3241 ccgaataaat ggtgttcttc tgccaactcc tcaatcataa gtcacaatat tcaacaaaca
     3301 tgagaaactg ttttttgaaa ttgttccttg tgattcagag ttaaacgaca gtctcacaac
     3361 ctcttggtaa acggcgttca atcagctgac cctcggtatt agctcaaaaa acggtaaaat
     3421 atgagaagct atttttccgt tttgtttctc taatacttag gataaaacgc tgattttaca
     3481 acctctgtta aacggtgttc aatcagctgt ccttcggtat tagctcaaaa aacggcaaaa
     3541 tatgaggaac tatttttccg tttgtttctc taatactcag gacaaaacgc tgattttaca
     3601 acctcctgat aaacggtgtt caaaagcttg catctgcggt aataagccca accaaacgaa
     3661 aaatatgaga agctattttt gtttggctgt tcttatgact tgatgcagga cagctttttc
     3721 acaacctctg atttcttatt cttcttcatt tgtagcaaaa taattacggt gataaatgat
     3781 acaacataca ctgataagaa caatggcacc tgcaaaataa aacgcatagc tttcaccaat
     3841 atgttcacca atcacacctg caataaatgg tgctaaagct ccaccgaaaa agcggacaaa
     3901 actatagctg gacgaagcaa cgtttcgttg cagacctggt atttccatag caatcgtcgt
     3961 caacagtgta ttgataattc cttggaaaaa tcctgctaac acaatgctga tcggtacaag
     4021 gactggcata gaagttccag ctcctaataa cagcaaacaa ataatgaata agacaaatgc
     4081 tgctaaaatt gtccgataag tcgtaaactt ccgctcaaga gccggtgcaa cgaaaatcga
     4141 agcaatagcc agaagcacac cccagccaaa aaatacaaaa ccgacttcta attcagaata
     4201 gccaatcaat aaaaatggcg catacgcaag taaagtaaag aatccgaaat tatataacaa
     4261 tgcgattagt ccaactgaac gaagtttatg attgcttaac gctttgacac cttcccaaag
     4321 gcctactttt ctctttggtt tctcagtttc aggtaaaagt accgctaggg ctataaaacc
     4381 gcaaaacatc aaaaatgcta ccccaaaaaa tggataacgc catgaaccac tgcccaagac
     4441 cccaccgact agagggccaa cagccattcc gcagccaagt gctgcttcat acatcatgat
     4501 tgctttttct gtgttaccaa ctaaaatact gacgatcgtt gctaaagcag tagagacaaa
     4561 taaagcattc ccgacacccc agccggcacg taacccaatc agcattccga tattcccaga
     4621 taatccgcca agaccagcga atacaacgat gataaacaat cctgtcatca atgttttttt
     4681 caaaccgatc cgactagata agaaacctgt aaatagcatc accacaccag tgactaacat
     4741 gtaactagta aataacaggg tcgtttcagc tggcgtagcg tttaactgtg cagcaatcgt
     4801 ttttaagatt gggtctacta gacccactcc cataaaagcg atcatacagg tagctgctac
     4861 aactagcgct gaacttttcc cctttgtttc ttcatttgtt tgttcttgta tatctatttg
     4921 attctccaat atttccatcc ctttctctct attagcatca tacatgattt attttacatg
     4981 tcaatatgca catgtaaaac gtggaaactt tactgtgata taataaagaa aataagacga
     5041 gaggtaggat ttttatgaat actttagcct atatattatt atgcgtgctt ttatccaaac
     5101 cacgttctgg gtatgaattg aagcagctga tcacaatttt ttgggaagcc catcacagtc
     5161 agatctatac aacgcttgcc aagttagacc agcagggtta tgtgaccgtt ttggacaaag
     5221 acgagcactc tcaaaaaaag atctaccact tgactgatga aggacgtgct cttgtagagg
     5281 aatggatcaa agaggaaact cccgctccta cgcaaaaaga tgaatttcta gcaaaaatct
     5341 atgctttttc gacgctcgat aaaacaacag ctactggact gctattcact agagaacaac
     5401 agctgaacaa aatcatgcaa aaaaatcaaa aaaaattgaa tgacctctcg ctagacaaga
     5461 aacaagaatt tggacgttat gttgtgatcc agcgcagaat tatgctttgt cagcaggaac
     5521 tccaatggtg tcagcaagta agagaacaaa tgcagcagtt ttttgagtaa gggataaaca
     5581 gaacctttct agaaatatct attgaagagc aaattatctt caataaaaga gcaatacagt
     5641 tggaaaagaa aaagcgatcc gaacgacagg ccaaaaccta tcgttcggat cactattttt
     5701 ctagaactgc attcaagaga ctatgacaaa aagcatgcca aggccttttt tcaaataaca
     5761 gtgttccatc attcattact ttgaattgct tttagcacac ttcttgaaag cgggccaacg
     5821 atgatcaact gcaacggtaa agcggcaatc acattcattt tccaagcatt catataagca
     5881 gagaataatt gatcaggtat tccttgttcc atgatcacac cgaataaaga catacatgtc
     5941 accattccta aaaccatcaa gcatgaaata gttaagatca tttggacctt gctttctgga
     6001 tttactggta gtttaaaggc aatttttttc gccagcacac cgatgatgaa gacatccaaa
     6061 atgaaagcca ccacgaatcc aggcactaga ccaatcaaca aacttgataa atgaagatct
     6121 tgatgcagcc ataaattata cgcgctcatc cccaagacca tcaaaaaaca catcattgtt
     6181 gtaaaaatta ttccttcttt tttatttgta ggcaatctgt accactcctt taaatttgtc
     6241 acgaaaatca tcgtagcaca tttaaaaatt tctcgtaagt acttttttta ttttctgccc
     6301 ttttatttca taaacttcat cagccgtacg ctcgacaaag taagaatcat gagaagtaaa
     6361 caaaatcgtt cccggatata tttgcattag cttctctaaa gcttcgatcg ttgccatatc
     6421 aataaaatta gtcggttcat ctaacagcaa cacattactt ggcttagtaa aaagcaaagc
     6481 aatcgtcaga cgggtcgctt ctcccccact taatgtacat aacggtttcg tcaaagtttc
     6541 ctgagcaaac cctaagtgat tcagcaaact acggacaagc gattctgaaa actctgtttg
     6601 tctcatgaca aaacgaatga tggattcttc agatgtcatt tgataaccct tttgctgata
     6661 tacttgaaag cttactttcg gagagagaag gattccttct ccttgtttgc ggatatgttc
     6721 taataaggtt gtcttacctg aaccattttc accgacaatc gcgatttttt tacctaaaga
     6781 aaactgaaaa tcaccattta ccagtaatgt tctcccactt ctttccaatt ggacattttg
     6841 tcccatgatg ggatagcggc tgtggagttc aagagatttt ggtactggaa aacggatttg
     6901 tttcatctgc tgcggcttgg ttgtttctcc tattttttgg agtcttctct ctaatgtttt
     6961 cgcattcttt tggatggctt tttgtacact gtctttttgt ttagaggaag acaaccgatc
     7021 cagtctgatt tgttgctttt tctttttact ggacactttt tccaactttt ccgcttgttt
     7081 tttcttttct tggatcgatt tttccaatcg ttttttctct ttcatgatcg actctgcttc
     7141 tcgtagctga gtctttttct ccaattcctt ttgagtgaga taggcactat aattccccga
     7201 aaactctcgg atttctccgt cttttacttc ccagattttc gatgctagct catctagaaa
     7261 atagcgatca tgactaacaa aaaccagcgt accataataa taacgaagct cttcaatcag
     7321 tcgatcaata ctttttctat ctagatgggt tgttggctcg tctagaagca tcccttgttc
     7381 ataagaagaa agaagacgta tcaactcgat ctttcgttct tcccctccgc tccatttccg
     7441 ctctgaagtt tcttccagtc ttaactggaa atgactggct aattccaaat ttaggtcctt
     7501 tgcttcagcg agataggtta attgaggcag gtagttgaag gtgatctctg tttggatttt
     7561 cccatgatcg ggaaaaagtt caccggctat tagtttgagc aaagtcgatt taccgacacc
     7621 attttctcct atgatcccta tacgatcttg ctgatagaca cgaagagaat caatagatag
     7681 ttctagctgg ttgccaaagc tgacttgtag atttgttata tttactgcta aattttccat
     7741 atgttttaca ctcctatttc atgtaaaaca acaattatcg atactaaaaa agagcagaaa
     7801 aatctgtttg caaaaaaact gcatcacatc ttttcctact ctgaacgaaa taactatgac
     7861 taaatcacca aatctgtcat agctttcacc tattctactg tcgttcgaaa aaatggacat
     7921 actccatcca tgaaattgtt gtttatacat gtcttttgaa tttatttagt tgttattcaa
     7981 aagttcgaaa cgtaatttca tcgatgcagt catttttccg acctcctttt ttattaggtt
     8041 accacacacg attcaaaaaa agcaatagct actgaatagt aactgaaaac cttttattcg
     8101 aacatagtga tcttttctgg tgctaaacga acagactcgt aaccttcttc taccgctttt
     8161 cctattgata aaataataac tggaacataa cggtctttat ccaaaccaaa tgcctctgcc
     8221 aactgatctg cttcgaatcc accaattgga tttgtttcat agccatgatc acgcgcaact
     8281 aacatgaatt gcatagccgc taagctagaa tcgactttga ctacatcgtt catttcttct
     8341 cttgaaaaac tcttgtaata aggaatgatc gcagctaatt gttgatctct gacttctttt
     8401 ggcatcttcc ctgattcgta agcttggttg tagatttctt cgccatattc gtaacattgc
     8461 atatcgccaa aaatcatgac cattgcagaa gaagtatcat tttgtctcgt attgaaacgg
     8521 atcaaaggac gcaagatttc ttttgcttcc tcgctttcag cgaccacaaa gcgccatggt
     8581 ttcatattga cagaagaagg tgcagtcgtc gcttctttga tcatttccag catttcttcg
     8641 tgtgggattt tgtatgtttc atcatatacg cgaacggatc ttctaccagt cataattgtt
     8701 gaaaaatcat ttttttgttc cattattatt aacctctttc gtttctgttg ttatattgca
     8761 aagttgggta ttcaatttat ctaaaagtgt tagaaattgt ttcatttcta catcgctgaa
     8821 agccggggat agcacggaac ctgtatcttt atggtccatt tcacaatgct tgattttctt
     8881 ctgggctttt tctgttagcg ctacatacac ttcccggttg ttctgaggat ttctctgacg
     8941 cgaaacatag ccatttgctt ccaatacctt cagatgacga gtaactgctc cttgatcaat
     9001 ctgcaacttt tcttgcaaac tgctttgtag caatggattt tcttctttca gtctcatcat
     9061 gatctcgtat ctagtaagac ttataccgat attttcttca aagatctgga cgattttttg
     9121 atcggctaat ttgatttgat ataaaaggtg actaatctct tctaaagaca taatttcctc
     9181 ctttctaata attgactcgt caacgattga cttgtcaata atagaataaa aaaagaaaaa
     9241 agtcaaagaa tcagctgtgt aataaaaaaa acgctctcaa tagaaaagta tagagaaaag
     9301 aaattttttg tagaatgaac ggaaaaaatg ataaagaaag cacaacgcta aaaccaagat
     9361 tattgattct tatcaaacta tagaagatag ctcttctgca gtagataatt ttacaccgcc
     9421 aaaaatttcc gaaatcgcct tttgcaagaa tcccgtcgaa tattagaaat tggatcagta
     9481 tgcttaaaca gtaataaaaa agttatgaca agatgttgtc acaacctttt ttgagtaaac
     9541 ggtgttcaaa aacttgcatc tgcggtaata agttcaacca aacaaaaaac atgaaaatct
     9601 gtttttgttt ggctgttctt actacttgat gcagggcagc tttttcacta cctcttttta
     9661 ataaccataa aatcctattc cttcatatct ccagcccatc ggtgttagcc aatctctttc
     9721 cttagcactc aaggtatagt gatgggtacc agttttttca tttggattaa atagtcgata
     9781 aattggcttg gaaccaccag aataaaaact tagaccttca taacgccaac cgatttttgt
     9841 taaagagttg atctcagatt gtgccattgt gtaatgatgg tctccactgt aaggattgta
     9901 taaacggtag acaggttgtc cgctgtttgg tgcttgccaa gcaatccctt catattccca
     9961 gcctacagtt ggcagataat ttttttctcc ggcattcaat gtatataaat gttctcctgt
    10021 atgacggtta tacagacgat acatcggaga agttgaagtt ggtgctgtat catactgtgt
    10081 cagattatag gtttcgatta cattgttcaa tgtagaacca taattaggcg cagtggcata
    10141 acgacctgtt agccaagccg tagcatcttt gtaagagttc gtattgcttt tccatgcacc
    10201 ggaatagtag taaacgcctg gctgaaaaga agtcgtcttc aataccgtta cgttatcttg
    10261 caatgattct ttgtacgaag gatattttct aaaagcggca ttcaccgtaa tccattgccc
    10321 attcacatat tccgaagtag gcatcgtaac agattggcca ttatagcttc ctttaatccc
    10381 aaatagattg taattagggg cagaagccaa agtactgttc ccccagccac tttcaacgat
    10441 ggcttgagca atcatgactg atgcatataa gtcattggca cttgccaaac tttgtgcagt
    10501 tgaggcaata ctatcaatga acgcttgttg agaattagca gatgagcgag cataaaaagc
    10561 atagccagta tagttttcga attcagacaa atctgcttga tgggtatgtg tcaatgtttg
    10621 cccatctgtc aattggttcg tgatttcttc tgcattctct ggattgttct gagcttctgc
    10681 tgatgtactg tcttcggttt gtgtttgttc tgttgtttca gttgttggtt cttcggttga
    10741 aattgtattt tctgagacag tagctgactg caaagtactt tctgtatcgt tttcaattcc
    10801 tgtttctgaa gaactcgatg tctgagttgc gacagtttct gtcgattctt cagctagcac
    10861 tgttgtgttg gcaactgcta gatttcctaa aagaataacg ctaaataata aattctttgc
    10921 tttcatcttt tttctccttt gaattatgaa acttttccta aataacagtg taactaaaaa
    10981 agtaaaagca agtcaatgtc catatttaaa taacatttgt ttctttttaa taaatttgct
    11041 ttttctgcat aaaaagacgt aaatgaagaa tattcttctc cattcacgcc ttaaattgat
    11101 ggtcgtttga ataactattt aacaagtaat tctctctgga tattatccgt tatggattat
    11161 acttcctgtg tcgtcggacg gataatcatg ttgctgacag acatccggtc tggcgtttct
    11221 atcgcaaatg ctacagcaga agctatatct tcagatgtta atccccattc tttttgcgct
    11281 tcatgaagtt caagagcagc ctttttgtcg ctgatggttt gatataattc agtttgaaca
    11341 gctcctggag agatgatcgt agatttgatg ttattttgcc gttgctcttg acgtaatcct
    11401 tccatgattg cacggacagc aaattttgtt ccgcaataaa cggcggaatc aggataaaca
    11461 acatgtcccg ccacagaatc agttgaaatg atatgcccag atttttgttt ttccatgatt
    11521 ggaagtacgg cggaaattcc atttaacaca cccatgatgt tgatatcaag catattttgc
    11581 cattcatcac ggtgaccttc aactaatgga gcggttggca tgattcctgc attattgtac
    11641 aatacatcga ttcgaccgaa cttttccatc gctaaatcga taactgcttg gacttgtgca
    11701 aaatctctta catctgctgt ttgaatatat agctcagctt ctggtaaaga ttcttttatt
    11761 gcttttaggc gatcttctcg gcgtgcagct ataactagtt tagccccttt ttctgctaat
    11821 agcttcgttg ttgcttcacc gattccgctt gaagcgccca tgatgacaat cactttttct
    11881 gataaatcat tcattctatt ctcctcctgt tttgaaaaat ctcttgctta tactcactat
    11941 ctcttatttc atcttactgt tcaaataatc cgttcgacag gtttgacgat tttggcaggg
    12001 attcctgcaa cgatggtatt gtctgggaca tctttcgtaa cagttgcatt cgctgcaaca
    12061 atggcattct cgccaatcgt tacgcctggc agaatagttg ctcctgctcc gatccaagca
    12121 tttttcttga tgtggattgg ctttactgac aagcctcttc gttcttttgg atcggttaga
    12181 tggttcaccg tgatgaggtt gacacgagga ccaatcaata ctgaatcttc taaacagatg
    12241 ccacctaagt caacgaacat cacttgacga ttgataaaga catttttccc tatactaata
    12301 tgtttgccga aatctgtata aaaaggcagc agaacagtca ccgtatcatc tactttgtca
    12361 ctaatgatat tttctagccg tttgcggata tctgctggtt catggtaccc agtattcagc
    12421 gctgctaaag gctcttcgtt ttccgcttgg atctgatgga tcacttcaaa gagcggatcc
    12481 gttactcgaa tctctttatt gacgatttgt ttttcgatga ggcttgtcat tttgctccct
    12541 ccttttatcc ttactttata acggacggct tatgatgtct aatacttata aaacatactt
    12601 aactattcct tttaactata cttagtggta tgctttttta aaaacaggag ggaaaaaatg
    12661 gatattcgta tactgacgta ttttttagca gtagctagag aaaaaaatat cagccgagca
    12721 gcaaaatctc tgcatatcac gcaacctact ctctcaaaac aactgaaaga tttagaagaa
    12781 gaacttggtg tgacactttt cactcgaggc tcccgagaga tacagttgac ggattctggt
    12841 cgatatcttt attcaaaagg aaaagaaatc ttgtcgttgg tagaaaaaac ggcgaacaat
    12901 ttaacaaatg acgcaatcat ctctggagaa atctacattg gcggaggcga aactaaagca
    12961 atctctttca tttctgatag ccttcattcg ttgatcaagg cgcatcctga tatccagttt
    13021 catttatata gcgggaatgc agatgatatc atagacaaac tggatagcgg attacttgat
    13081 tttggactcg taatcgaacc gacaaacaaa caaaaatacc agtcatttcg actgccagcc
    13141 caagatcgtt ggggactttt gatacatcgg aatcatcctt tagcaaagca aacagtcatc
    13201 catccgcagg aactgaaaac tgttcctttg atcatttccc ggcaaacgtc agtggacagt
    13261 caactagctg aatggctagg tttcagcata gatcagttat cgatcgtcgg aacatataac
    13321 ttattgtaca atgcttcttt gatggtcgat aacgggactt ttggtgcatt agctctagat
    13381 gggatcatca atacttatgg gacggaaaaa gtattcatcc cactcgatcc gccattacaa
    13441 gcttcactga atctcatttg gaaaaaagac cagcctcttt ctcctgctgc tcgtaaattt
    13501 ttagatgtcc taacggagaa atacagttaa gtagagtttt tttccttcgc tttcagcttt
    13561 ttaacaacga ataagttaag caaaagatcc aatgattaaa taacataagt ttcttaattt
    13621 gttccttcct tttatttctg ataattccag taaattacta taattataaa aaaagaattg
    13681 acaaaacata actgtctata taaaattgat tacgttaaca ataattcgtt tcatgtgact
    13741 agatcatact aggcatgaaa aatctctgag gaacatggtt tccctaggag gtttttcgtg
    13801 tctatttttg tttggccgca atcaactggt ctagcaaacg tgaaaaccaa atcttcaaaa
    13861 agggtgcgcg catgaaaatc aaaaagatct ttaatcaaaa tgcagtattg gtagatgacg
    13921 gtggacagga aaaagtagct attggaaaag ggattggatt tgacagaaaa agaaatgatt
    13981 tgatttttga tcgtgatgtc gaacggatat ttgtcatgga accagaaggt caaatcaagt
    14041 tgcaaaattt acttaatcag attgatgaac gctttttatt tgcagcagaa acgatcattg
    14101 atcatgcgga aactgttttg atggaaaagt tgaatgaaca tgtattgatc gctttgaccg
    14161 accacattgc tttttctgcg gagaacatta ataacggtat cgtcatccgc aacaaacttt
    14221 taagagaaat cgaagtgctt tacagtgatg aattcagtat tgctcaatgg gcagtagagt
    14281 atctgacaaa agagcttggg attccatata cttatgacga agcaggatat atcgcaatcc
    14341 atattcacag cggacgaagc ggtcggaaca ataaccatcg cagtatccgt gaagtgacga
    14401 ttgtatctga catcatccat ttagtggaat cagaattgga aacagatatc cattcagaac
    14461 cattttcact gaattattca agactggtga atcatttacg actgcttcta caacgaaccc
    14521 atgcgcaaca atatgctgtt ttagacacgg aaatcgtaga aatggtcaaa cgaaaatacc
    14581 cagaaagcta taagatttct aaaaaaatcc gtgtgttgct gaccaaagaa taccagctcg
    14641 cgatcacgaa agaagaactg ggttacttgg ccatccatat cgagcgttta agaagcgcaa
    14701 tcgtacaaac aaatgttaac aatcatgagg aggaaaaaaa ttgaaagcat atctacaacg
    14761 aatgggacgt tcccttcaac ttccagttgc ggtcttacct gctgctgctt tactagtagg
    14821 gatcggaaac tggtgggcat cttatagtaa cgacattgtc gctcattttt tacaagctgg
    14881 cggtaacgca gttttaggtc aattaccttt gctgtttgcc gttgggttag cactagggat
    14941 gtcagtggac aaatcaggtg ctgctgcctt ggcaggtttg gtcgcatttg aagttccagt
    15001 aaatgtttta aaaactgatt cagtagctac tttattgaat atcaaaccag aatcagttga
    15061 tccatctttt gctcagatca gtaacgtatt tattggtatt attgccggat tgattgcggc
    15121 agctctttat aatcgtttcc acgaagcaaa attaccgatg gctttatctt tctttagcgg
    15181 caagcgtcta gtgccaatct tggctgcctt tgtcatgttg ctgatcagtg cagtgctact
    15241 gctcgtatgg cctccggttt actctgcttt agtttctttt gctaaattca ttttgagttt
    15301 aggctggggt ggtgcaggtc tttatggctt cttcaaccgt ttactgatcc ctacaggatt
    15361 gcatcaagca atgaactccg tgttctggtt tgacgtagca ggaatcaatg atattggtaa
    15421 atttttggct tctgaaggaa caaaaggcgt aacaggaatg tatcaagctg gatttttccc
    15481 aatcatgatg ttcgggcttc cagcaggagc atatgctatt tacagaaatg cacgtccaga
    15541 aaacaaagca aaaacagctt ccttgatgtt agcagcagct tttgcttcat tctttactgg
    15601 tgtaacagaa ccacttgaat tctcatttat gttcgttgct tggccattat atgtcttgca
    15661 tgcaattttc actgggattt ctttagcagt gagtgcattc ttccattgga cagcaggatt
    15721 tgcctttagt gccggatttg tcgattactt cctatctttg aaaaatccga ttgcgaatca
    15781 gccattgatg ttgatcgtcc aaggattagt aacagcagtg atttactact ttggctttaa
    15841 ctttgctatc aaaaaattcc atctgatgac tcctggccgc gaggaagcag atctttcaga
    15901 tgacgatact gcaacgacta attcagacaa taaatatgcg gcacaagcaa gcaaaatcta
    15961 cgctgcatta ggcggagccg acaatgtaac ttcgatcgat aactgtacta cacgccttcg
    16021 tttgcaagtc aaagatacag gcctgatcga tcaaaataaa atcaaagcca ctggcgtacc
    16081 tggtatgaag atcatcgacg gaaaaaatgc gcaagtcatc gtaggtacag aagtacaatt
    16141 cgttgcagat gagatggcaa aactacatgg tggtgctgca acacgacctg cacaaacaaa
    16201 caccgtggtt aaaacagaaa catttgccgg tggaacgatc gaacaagata tttacgctat
    16261 cgcaaatggg aaactgattc caatcactga agtttcagat gatgtattcg cggaaaaaat
    16321 gatgggtgac ggctatgctg tattaccaga aaatggagaa atcttctctc caatcgctgg
    16381 tacgatcaca aacattttcc caacgaaaca tgctgtcgga atccaaacag atgctggtat
    16441 cgaagtcttg cttcatatgg gcatcaacac tgttgactta aaaggcgaac cattcacttt
    16501 atatgtaaaa gaaggtcaaa aagtcgcaag aggtcaattg attgctttag ttgatcttgc
    16561 cgctattcaa tctgcaggca aaaacacgga tatggtcgtt gtattcacaa atggcgacaa
    16621 agtccaaagc ttagaaatcg aacccgcacg tgatgtaaaa gcaaatgata aaatcggctc
    16681 tgtttccaac aaagcctgag ccatcgtctt ccccagactt ttttatcgat aaacaaacag
    16741 gagctgagac aaaaatcttg tctcagctcc tttttccgaa taaaaacggc gttctcctgc
    16801 caactccgcg gtaataagtc cagccaaacg taacatatga agagctattt tttatttgac
    16861 tcttcttatt acttgatgca gaacagcttt tttacaacct ctgataaacg gtgttcaaaa
    16921 accagtttat cggtatgaag tcaaatttat gcaaaaatgt ggaaagccat tttcacaaaa
    16981 tttttcttca tactcaaaac taatcggctt tttcacaacc tctttataca ttattcctga
    17041 tccaatattt cttttaatgc tgcttcaaga gaagttaatg gttttcccaa tactttttct
    17101 aaatcatcag aggaaatagc taactgatcc tttttaatat catgttggat cgctaaaaac
    17161 atgtcagctg cttcttgagg gaatccggct tcttttagat tttcgacaaa cgctgcatca
    17221 cttgcttcaa taatttctaa ctttttacct gtcgcacgct ctaatgcctt ggctaattct
    17281 tcatagtcag ttagattgcc ggataattct aagatttctg gagagtcact ttctactagt
    17341 acttttgctg cagcttctgc atattcacgt ttcaatgccc aacctgtttt tccatttcct
    17401 gcagcataaa cgaattgacc agtttttaaa gcctgcccaa tgataggtaa ttcattctct
    17461 acataccaat tattccgtaa aaatgtatgg gaaatacctg aatccttaat cattttttca
    17521 gtgaattggt gatctggcgc taatacactg atcgagtgat cggcatctgc aaaacttgta
    17581 taagcgatat aagatacccc agctgcttgt gctgccttca ctacattctt atgttcggct
    17641 tgacgatttc ctggaacgcc agagacaaaa agcaatcgat ctattccttt caacgcttct
    17701 ttcattgagt ctaaatcatc ataatcacca atacgtatat taaatccagc ttctttcagt
    17761 gcttctcctt tttcttcact acgtactagt acatagattt cattttgtgg aaccatcttt
    17821 tttaagaatt ccagcgcata accaccaaaa cctccagttg ctcctgttac taaataatgc
    17881 atatttgcca ctccctattc tttttatttg ttaagattta cagctgtaca tttataaatt
    17941 acacctttat cataaagagt gtggtaagat aagtcaagca aaaaaaatga aaggatgaca
    18001 actgatgaag tattctctgc aatttagtga tgcaatccat gtacttgcct atattgaaat
    18061 gtttcaagat acccgatcac tttctagtga gatgattgcc agtagtatcg aaacaaatgc
    18121 agcaaatgta cgtaaaatca tgagtcagct caaaaaatcc ggtttgatct ttacaaaaac
    18181 cggaaaaccg cagccttccc ttgccaaaag tccagatcag atttctttac tagatgtcta
    18241 tcgcagcatc gaaggtaata cacagcttat ccaagtcgat ccaaaaacaa atccaaattg
    18301 cgtcgttggt gcgaatatcc aagaagcttt agaagaaagc tacgctcggc tgcagaaaaa
    18361 ggcagaagaa gaaatggcta agatcacact tgatagtctg atacataaaa tagccgtttt
    18421 ggaaaaagaa aaaagaccag aaaacacagg cctcgtagag agatttttat aattctctct
    18481 actgtgcctg catttttctg atcggaatcg tctacataaa ttcttttatt ctccggggga
    18541 caaaagcctt gtcatgcccc tttttcctct ctaatgctca ggtcaaagcg ctgaagtcat
    18601 gacgtcttga taaacggtgt tcaagtagct gaccctcggc attagttctg aaaaatgcaa
    18661 aatatggaaa gctatttccc atttttcctc tctaatgctc aggtcaaagc gctactttca
    18721 cgacctctat attatcatgg tttattgact accggtatcc agatttctcc catatatttc
    18781 ccgttggtaa ctcccatttc gactacagca tttggacccc caacataggc gtaatcttct
    18841 acttctctta aaacatgccc aaaggccgta ttcgtcaaat catttcttaa ttcttcaggc
    18901 gattctgcta aagcgattac aacaagatac gttccttttg ggaatgggat gattcgggta
    18961 gtgtttggaa catcctgact cgtttgaaca ccgacatagt acatcatttt cccgttatag
    19021 ggttcgttga ctgtaaagaa ataatgattt tcagcagctt gtttcaatgc atctattgtt
    19081 ccattaccga taactttttg ctcaaatgct tgtttctctg cagccaaacc gacaaaatcc
    19141 gtattgctac tcttcaattc cacaccaata cccgtcaccg taaaatctgg tttttcttcg
    19201 atcctatact ttgccatttc tcccaccctt tctattatat taagttcatc ttacataaaa
    19261 ttaaagggaa atgacacgat tacgctcttt tttctaggga tatttttatt ttttaactat
    19321 ttttttgctt tcactgcaat ccaaatctca ctgtaaacat tactgaaatc ctctccccat
    19381 tttgtaaaag agatctcggg agcttcgact aactcataat ctgaagaagg aagccattct
    19441 gcatagattt tcccccaagt atcttgtaaa gtttggggaa atggcccttt attaggaaaa
    19501 atggcccaag tgtgttcttc tatagcaagc atttctaaat cagcgtacgg attttctttt
    19561 gtcgtagcaa agccaatcat gtgcatcaaa ctgccctttt cttccaagcg ggctccatca
    19621 aatccataag aaacgttgat tacttgattt ggatataaat ctcctaactt atgcatctct
    19681 tccctttgag tctccgtgat cgtctgcgct aacttttgta tttcttgatt gactccttca
    19741 aatttgacag gaactttttt cgccacacct gccacataaa actgttcttt cttttcgata
    19801 cgaaattcca tcgattttcc tcctctgaca tcaataaaaa atgtcaagcg tggaaaagat
    19861 ttttgtatgc cgtatctgct gatttctgaa ggcagatatc ctgaccaatc acgaaaagct
    19921 cttgaaaaac cttcgactgt ttcatatcca tatctaaacg ccgtttctgt cactcttgat
    19981 ccatctagga gatcgcgatt agccaaagcc aatctacggt ttttgatata gtccattagc
    20041 gacatccctg caataaacgt aaagatgcgt tttaattgat attcagagat tcctgtattt
    20101 tcggctatta ttttatagga aagtccttct gtcaaatgtt cttcgatgta atcgatcaat
    20161 tggttcagat ctttaagcat atttatcttc ctccttgctt gcctttttat catattatca
    20221 aagaaagtca attacttgat gaatcaggaa ccaatttagt cagatgtaga aaatgttgta
    20281 tggatctaaa gaaaattctc aagcagcgtt tcgtaacgga taattggtat tatctgccta
    20341 tctatgtttt acctttagaa aaataaattt tggggaagac acattctaag taaacaaagc
    20401 ggtaaaaata tcttttattc ccttagttaa tccgcttact acctactgta caaagcaaat
    20461 tgttgttcaa actgctgatc agaacgctgc ttggattttt ctttgcgata catcatacct
    20521 cttttatcaa ttggaacaat tatttatttc taacaaaatc acttcatttt tgtcagataa
    20581 ataaaaaaca aaaaaagttc catctttaaa acacgctatt cttttacttt ttctcacatt
    20641 ctctaagaaa agaaaaggag aatgaatttt tgacaattcg agcaaaagtg attatcaaat
    20701 atgtaaaatc agctactttt ttcttaaacg tttctttata ctaaatataa ataagtgttt
    20761 catgaaagga gaactagatg aataattggc ttgagtattt tcctgaaaac gttttggaac
    20821 gcggttacag ctatcatctg cacggttttg tccgtcattt gaactataca agcaagtact
    20881 tgtccgcaac agtttctggc acggaagatt ataaggtagt gatcacttgg gatgaaaaga
    20941 ccaacatgac ttgtgattgt ctatatgcca tagaaggaaa aaaatgcaaa cacatggctg
    21001 ctgtcttatt cgcttacgaa gaacgtccaa taaaaaaatc caactactca ctttccgaac
    21061 tctcgtccct tgtttcctcc gctagttcct ctcttgtacg ggagctttta acagagattc
    21121 ttatagaaca tccgcaattt atcgaacgct tcaaagttaa gatgccattt catgcaataa
    21181 attattcaga taaattaaca actatcatcc ataaatacga tcatattatc aagaaaaaca
    21241 aaaacaggaa aacagcgaag ttcatcatgg agatgcgaaa gttcatccaa gaagcagttg
    21301 aatcgctcat tcagcaaaat gcctatcttc ccgcattcga attgatcaat gaagtgattg
    21361 ctactctcga aacattctat tgggagcctg aggatgaacg aacccttctt ctgatagagg
    21421 actgttatta tttatggaaa gaattattag cagaagcacc tcatgcagaa aaaaggcaaa
    21481 tgttctcttg gttcgtctgc caagtcgatc atactgacgc ctcttattca aaacgctact
    21541 ccataaaaat cctgaaagaa gactttcggg aaaaagaatt ttctaaccaa aagaaaaaaa
    21601 tagataaaaa aactaaagaa acggtaaaga aagacgattt caatgaaaaa tagcaagtaa
    21661 acgataacaa ccatcctaaa tccacaagaa atacagcaga ccttgcttta aaaacacgaa
    21721 tcatgcaaag gatgaagtaa agaagactta acgcgtaccc aatattagtc atttgactat
    21781 taggtacgcg ttttgtttta taagtcatga caaatttcct tcgttgctgg gtagattttc
    21841 cggcattgtt gatagatttt atgatacttt tctacatttt gtaagagagg ttccactgca
    21901 tctgtatatt ggataaaatt ctgacaacaa ctttcaggtg tgtcgaacca ctctaatctt
    21961 taaagataaa tattaaaata acgattaata atattaacag tctttatttt tcaccaccaa
    22021 aaacctatct ttgcatatac taaatagacg tatatacaca gataggtttg tcttgtgaaa
    22081 aataatgatt atccttacca aaaaatgttt gtttttctac atgttcgtac ttatcaaaaa
    22141 atatgatcct tattgaaatt actggctatt tatattccaa cgacttcttg tgttaatttt
    22201 tgttatctca tagtaaatta tgtttcattt tcacttaatt tagttgtcac gtgttcgtca
    22261 attcgtaaaa acggtaaata tataaacaca cttgcggcta taattaatat ttgtaaaaca
    22321 gctgctcgcc agtctccggc tgttgctaaa aatcccgata taactggtgg cgtagtccaa
    22381 ggaatttgca ctactacatg attaattaat cccacagcag ttgcaaaata tgctactgtc
    22441 aaagaaaaaa tcggtgcaag cacaaaggga ataatcagac tgatattaaa tacaataggc
    22501 aatccaaaaa taataggttc gcttatattg aatatagcgg gagttaccga taatttagca
    22561 aagtccttat aatcttttct acgactgaag ataaatattg caatcaataa agcaatcgta
    22621 tttcccgatc cacccattac cgcaaaggta tctttaaagg ccatattgat aatatgtgga
    22681 atttgctctt tgtttgcata agctgccata ttttcttgca tattctgaat caaaaacgga
    22741 tctaacaatg aacctgatat tactgcttga tggataccaa atccaaaaaa tagattagca
    22801 atagatgtaa ttaacaaaaa tcctggtaaa ccagttgtaa ttttacttaa tggctctgta
    22861 actatcgtgg taattagctt atttatatcc atattaaaca attgaatgat tataaatgac
    22921 aataaagcaa acgaggtaat cgtaataatg attgggatta atacattaaa agactttaaa
    22981 acagcgggag gaatatttcc ggatagctga atctgtagtt tcttattatt agaaagttta
    23041 ataaataatt ctgtagccgc taatccaaca aatataccta caaacattcc tgaagctcct
    23101 gtataggata taggtatcac acccgtcact tcaacagctt tatccgcatt ttcaggagta
    23161 aacatcatct gcataggtgt taatacaaca aatgtcgaaa tagcaactaa taccgctgca
    23221 atatgatttg tataatttct gcttgcacac atatgataag atactgctgc tacaactaat
    23281 aatgtagtta tgcttaatgt accattggca attgaagtac caatttgttg taacgtggtc
    23341 aatgtactag tatcgacaat ttcccccatg aatcctgttg gttctaagat cactgaatta
    23401 ataagtgtca caaatcctgc taacatcata aatggcataa atgtagcaaa cgcatctcgc
    23461 aaagttctta aatggatctg attccccaac ttagtcgcaa ctataccaaa tttttcaaat
    23521 acaatgtcct tcctgctact tttgcccata ttttttctcc tttatgctta ttgtaatcga
    23581 ttactttttc cacccaaata atacatcact tatcaaaaaa ag
//
LOCUS       DADVXU010000038        23534 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.040, whole genome shotgun sequence.
ACCESSION   DADVXU010000038 DADVXU010000000
VERSION     DADVXU010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 23534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.040"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..1835)
                     /locus_tag="KB953_001391"
     CDS             complement(<1..1835)
                     /locus_tag="KB953_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBB6866375.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
     gene            complement(1832..2536)
                     /locus_tag="KB953_001392"
     CDS             complement(1832..2536)
                     /locus_tag="KB953_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBB6866376.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(2548..4857)
                     /locus_tag="KB953_001393"
     CDS             complement(2548..4857)
                     /locus_tag="KB953_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBB6866377.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(5052..5516)
                     /locus_tag="KB953_001394"
     CDS             complement(5052..5516)
                     /locus_tag="KB953_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866378.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(5516..6142)
                     /locus_tag="KB953_001395"
     CDS             complement(5516..6142)
                     /locus_tag="KB953_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBB6866379.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(6149..6514)
                     /locus_tag="KB953_001396"
     CDS             complement(6149..6514)
                     /locus_tag="KB953_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBB6866380.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(6504..6842)
                     /locus_tag="KB953_001397"
     CDS             complement(6504..6842)
                     /locus_tag="KB953_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866381.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(6832..7158)
                     /locus_tag="KB953_001398"
     CDS             complement(6832..7158)
                     /locus_tag="KB953_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBB6866382.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(7139..7417)
                     /locus_tag="KB953_001399"
     CDS             complement(7139..7417)
                     /locus_tag="KB953_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HBB6866383.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETD"
     gene            complement(7419..8783)
                     /locus_tag="KB953_001400"
     CDS             complement(7419..8783)
                     /locus_tag="KB953_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBB6866384.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(8796..9365)
                     /locus_tag="KB953_001401"
     CDS             complement(8796..9365)
                     /locus_tag="KB953_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HBB6866385.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(9337..10524)
                     /locus_tag="KB953_001402"
     CDS             complement(9337..10524)
                     /locus_tag="KB953_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBB6866386.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(10588..12315)
                     /locus_tag="KB953_001403"
     CDS             complement(10588..12315)
                     /locus_tag="KB953_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBB6866387.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(12312..12764)
                     /locus_tag="KB953_001404"
     CDS             complement(12312..12764)
                     /locus_tag="KB953_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HBB6866388.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(12876..13256)
                     /locus_tag="KB953_001405"
     CDS             complement(12876..13256)
                     /locus_tag="KB953_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBB6866389.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(13253..13639)
                     /locus_tag="KB953_001406"
     CDS             complement(13253..13639)
                     /locus_tag="KB953_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866390.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(13641..13919)
                     /locus_tag="KB953_001407"
     CDS             complement(13641..13919)
                     /locus_tag="KB953_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866391.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            13967..14695
                     /locus_tag="KB953_001408"
     CDS             13967..14695
                     /locus_tag="KB953_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HBB6866392.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            complement(14826..15302)
                     /locus_tag="KB953_001409"
     CDS             complement(14826..15302)
                     /locus_tag="KB953_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HBB6866393.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(15262..15609)
                     /locus_tag="KB953_001410"
     CDS             complement(15262..15609)
                     /locus_tag="KB953_001410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866394.1"
                     /translation="MNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVLKIE
                     QNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIRGGV
                     CEISLVKRLPRTR"
     gene            complement(15596..15781)
                     /locus_tag="KB953_001411"
     CDS             complement(15596..15781)
                     /locus_tag="KB953_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866395.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(15782..16054)
                     /locus_tag="KB953_001412"
     CDS             complement(15782..16054)
                     /locus_tag="KB953_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HBB6866396.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(16051..16254)
                     /locus_tag="KB953_001413"
     CDS             complement(16051..16254)
                     /locus_tag="KB953_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866397.1"
                     /translation="MVKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDK
                     YLGEGFCGDSLFDDERILKEETE"
     gene            16379..16591
                     /locus_tag="KB953_001414"
     CDS             16379..16591
                     /locus_tag="KB953_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866398.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            complement(16624..16818)
                     /locus_tag="KB953_001415"
     CDS             complement(16624..16818)
                     /locus_tag="KB953_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866399.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(16831..17013)
                     /locus_tag="KB953_001416"
     CDS             complement(16831..17013)
                     /locus_tag="KB953_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866400.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            complement(17010..17150)
                     /locus_tag="KB953_001417"
     CDS             complement(17010..17150)
                     /locus_tag="KB953_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866401.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            complement(17640..18005)
                     /locus_tag="KB953_001418"
     CDS             complement(17640..18005)
                     /locus_tag="KB953_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866402.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            complement(18045..18347)
                     /locus_tag="KB953_001419"
     CDS             complement(18045..18347)
                     /locus_tag="KB953_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBB6866403.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            complement(18347..18646)
                     /locus_tag="KB953_001420"
     CDS             complement(18347..18646)
                     /locus_tag="KB953_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866404.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(18639..18803)
                     /locus_tag="KB953_001421"
     CDS             complement(18639..18803)
                     /locus_tag="KB953_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866405.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            complement(18809..19078)
                     /locus_tag="KB953_001422"
     CDS             complement(18809..19078)
                     /locus_tag="KB953_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866406.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            complement(19090..19839)
                     /locus_tag="KB953_001423"
     CDS             complement(19090..19839)
                     /locus_tag="KB953_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6866407.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(19836..20528)
                     /locus_tag="KB953_001424"
     CDS             complement(19836..20528)
                     /locus_tag="KB953_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866408.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            complement(20534..21205)
                     /locus_tag="KB953_001425"
     CDS             complement(20534..21205)
                     /locus_tag="KB953_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HBB6866409.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(21198..21539)
                     /locus_tag="KB953_001426"
     CDS             complement(21198..21539)
                     /locus_tag="KB953_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866410.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            complement(21717..22415)
                     /locus_tag="KB953_001427"
     CDS             complement(21717..22415)
                     /locus_tag="KB953_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HBB6866411.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            22502..22972
                     /locus_tag="KB953_001428"
     CDS             22502..22972
                     /locus_tag="KB953_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866412.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            complement(23015..23194)
                     /locus_tag="KB953_001429"
     CDS             complement(23015..23194)
                     /locus_tag="KB953_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866413.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(23181..23519)
                     /locus_tag="KB953_001430"
     CDS             complement(23181..23519)
                     /locus_tag="KB953_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HBB6866414.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
BASE COUNT         6538 a         4960 c         3635 g         8401 t
ORIGIN      
        1 attctccaag ctctcttttc tgcatctgtt tttaattcat caggataccc accaggtgtt
       61 tctcctccgc cagatggacc gccattttgt cctggtatca cttctttacc tctaacagta
      121 agttttccct gaatatctaa atcaccgaaa taatatgctt ttccgtttcc taataacaca
      181 aatccttttt ctgcagttgg agaaattaaa atgtattttc catcgccatt tgtacgtatt
      241 accaaagaat tttcctcaag aggtgtaggt gttacagcat ttggaaatgg atttcctgct
      301 gaatctgttg ttccgatcgt tccaatggat tcttttctat tccagaattc catacctttt
      361 ttggttagct ccatgatttt tttgttttca ttccaaattt gaagcgtgcc ttttacaagt
      421 tttaacacat caccataagc attaaaggaa gtttcgaata cttctgcatt tattgttcct
      481 actttgatga agtcagcaac aatttctcct tttgatgtga tagcaatacc gaacgggcca
      541 tttactccgt tgtctgaata acctaagcca tttaaattcc aacgccacac tcgctttgca
      601 ttcgccacat ttggagtatc catgataaga atctctgatg gagctttttc tggacgaaaa
      661 acgacatgcc caccagaatt accagtgatc catgccgtcg cattcaacac attttgaact
      721 aacgtttctg ttcgattgtc gattttcttt ttcaattctt gtgcttgagt atttactgcc
      781 gaagtgtaca gtgataaatc attacctaaa acaatatcct tatatttccc aagcgttggg
      841 aaataagtgt attcgaccat acgctctttt atctcgatat ctagctcttt cgccctaaca
      901 tgagcgacat caccaaagtg taaagacgct agctcttgat acatgtcacc gtattcgagt
      961 gtgtgttcca acgccaccat actgactgta tgagtagctt ttggctcatg gatacgatca
     1021 ttgtcaaata aggttttgcc ccatttgatc aattcatcaa ctgttttaca atctccgttt
     1081 tcacgtttgg caatacgtcg attctcgttg gttactccat caatttctaa ataaccatat
     1141 tcgataggtt ctttgtcttc atcataatcg ttatcaggaa caccgccaac caaatacaaa
     1201 ctattgacaa ttgattcttc atcaatttct tcttcgattg cttctaaatt gatgccaaaa
     1261 tcaatccgaa aaccattatc tgatccaatc tgttttacca gtttcaaatc atagttatcc
     1321 atatcaagtt ccgcgctagt cacgccagtc aaattttgat taccgttatt tgaaccaatg
     1381 attgcttcaa ctggcgcgac ttgtcttgct gtaaattggt gcgttgtccc tacattcgaa
     1441 agatagttaa atttttgatc aaatgccaaa ctgtttttaa gattggtcat gatctgagac
     1501 ccattgccgt tatccgtaaa cgacttgaca ataaagttct tattggccat aaaaccaata
     1561 tgccgagctg ttacagaaac ggaggtcaaa ttctttttga cattataaat ttcaaaatat
     1621 tgccatgatc catctggtac cttcgctttt ataaaatttc ctttttttaa ataagagcga
     1681 tactgtccgc ttcttgaata gttaccataa aaacgatatt ggccattcag cacacgattg
     1741 atttctggca aatcttccca atcaactaac gacgctccgt tcacgctcaa atcatctggc
     1801 atttttttat aagcataaat aaattcttgc gtcacagata cacactccta ttccaaaacc
     1861 gcacttcttt aaattttccg gatattttta cctgattcca ttcaggctgc aatactggcc
     1921 aatcaccacg cgtaaataaa ttcaaacctt cttgtattgc ttttccaagc tgagtatcaa
     1981 ccacgattgt tgcagctaac gtattaagta ttgttaggct tttatcaccg acagcgatcg
     2041 ttatatctcc tccgttcgat tctatttcca gatagggatg tgcaatttcg tcaccatgat
     2101 caaaaatatc cataacacta gaatgaaaaa ttttaggtgc ttcaccgatt tttcttttga
     2161 gtggctgaca acgaaaagta acatcgaatg tataaaagaa gccccactcg ttttcgaaag
     2221 ggacttcttt atccatgctg cagatagcct ctagatactt gtctggatca ttatgtgtga
     2281 ttaattggct tttaccagtg agccatcgtt tgacttctct cagtttcgaa tgtggaatag
     2341 tgatcccttc tatttccaaa tcaaaaggtt catagtcatc aaacgtctcc gtcaattctc
     2401 cgcttcggcc ctgaatcgtg taagtttcgt atcgcttgtt cggcataata tctggaagct
     2461 ctgcctcaat gatgcaattc atatcaatta ctgcattccg atttttccaa ataaaatttg
     2521 gttcatctgg attcataaat tgtcgactca aataggaaca cctcccaaat cacgaatagc
     2581 ctgtttgttt gctttagcaa atttacgatt catacgatct aactcagatg gattgtttgc
     2641 atcgactttg ccaatgtgta tatgctgttc aatgctacct ccagaaactc ttccactgat
     2701 ccctttgcgt ttttcttcgt ctgataatgg agtaactgtg gttttaccat tcttggcagt
     2761 cagtaactca ggtccagctt caccaacaat cgcttgtcca ttgattagat gaccgccttg
     2821 tgctaaataa ggcagttttg aaatagaaaa tgattttcca cctacaccag gaacccattt
     2881 tggtatcttt atattattca atccaccaat aaatccattg atcaatccaa tcatggcatt
     2941 aataggagct ttccctacag caacaatacc atcgaaaata cctccaaaaa tatcaactag
     3001 accttgccat gcttttgacc aattccctgt aaatactcca gtgataaaat ctagaaaacc
     3061 actgaaaatt cgttttccag cattataaaa attattgaaa tttgctataa caccatcgaa
     3121 aaatccacca aaataaccac ttaaaaaatt aaatatttca acagctacat ctgaaacacc
     3181 tttgaagaaa gaattcactc catcatgaaa ccatttaacg ttattatatg cccaaattag
     3241 tccagtaact aatgcgccaa tagctataac tgctaatgca aaccagcctc ccgacattcc
     3301 aaataaacca gcaagtcctt gccaaactcc tataaaactt ttaacttccc caacgatttt
     3361 tgtaacagat cccataagtg aaccaagaac gattagtact ggtccaactg ctgccgctat
     3421 accagcaatt gttatgatcc aattctttgt atcttgatct agagaccccc accatttaga
     3481 gaattcctgt aataaggaag ttgctttttc aaaaaaaggc aaaaggctta cttggactgc
     3541 ttcacctacg tcagccattg ctaattttgc attgttcata gcttgatcgg cttgatcaat
     3601 tgggtcgaga gtagcatcga atgtgtctcc tacagctccc ccactttctc cagcagtttt
     3661 tgctaaatct tccaggttca aagtacctct acgaattgca tcggccattc taggaccgcc
     3721 tttagtcccg aatacttctg ctgctgcatt gatggcttct gtttcagaac ctgcattttt
     3781 taccttgtct tgtagttctt ctagtccttg acttaaagat tttccgtctt tcgcataagc
     3841 cactgttgct ttagataaac tacttaaagc tgcgcttgaa tcaacaccag attgctcaaa
     3901 ttgtccaagt aaagtgaccc cttcaccaaa gctcaaccct aattgtttaa tttgtggtgc
     3961 gccatcaatt gctttttgca tcaagtcgtc tacagattga ccagtattct gcgatgtttt
     4021 cgttgttaca tcaaggacag aattcaaatc atcatattct agtccataag catcaatagc
     4081 ttgtcgagca gatattgctg attgcgaaac atctgtatca ttgatttcag cgtactgtag
     4141 gagataattg gttgaatctt ccagtttttt atccatgaat ccaaattgtg tatttacctc
     4201 accaatagct tccccaaccg tttgtaaagg taaatgagta tttgaaccaa cgtttttgaa
     4261 agactgtgaa agtctatcag cttgatcacc tgttgctccg gtttttgtaa tgatggtatc
     4321 aagtgcctcg tcaacttcac caaatgctgc aagtcctgct gctcctgccg ctacaatagg
     4381 cgcggttact ccaacagaca ttttttcacc aacgcctttt actttctcac ctgtttcttc
     4441 gattttctgg agtttttttg ctgtatcaac agaaacgtca ccttgttctt taagtgcatc
     4501 gttcgtctgt tctaatgctg tacgaagttt attttctccg gtttcagaat tgagtaattg
     4561 tttataaagt ttttctgatt cagcagaata ttcaccggtt tctttgacag atttttcata
     4621 ttcctctctc agcatttgag atcgttgttc agccaatcct aattgttttt ctaatttttg
     4681 tttagccgct cgcaattttt ctgtttgagt tgcatccttg tccatcgcag acacttggtt
     4741 tttgtactcg gcagcggcta agttcatttc tttgttgatt tctttgattg ttttcgaata
     4801 actgacttcg ccattcgttt taaaattaag aacgacatca gattctttac cagccattta
     4861 tcttagcgct cctttctacc accaaggcga tttatccata gttacagatt gaggtggttc
     4921 aaaatcggtg ttttgttgta accactgtaa ataagatttg agccacaagt taggtgtaga
     4981 ctttaaaaag aaactctcac tccaattcaa tagagtgaga gcaacgtaaa tataaaaact
     5041 ccaaggagtt cctatctctt ccgattcttt ttgtttttct ttctttttgc ttgcgctttt
     5101 tgaaagtctt gtggcttctt ggattttttt aggtcttcca cctgaaatgt ctggctagca
     5161 aaaatttcca tacaggtact ataagcagac aacacctctc cactcattcc caaaaatttg
     5221 aaaatagttt ctggatcttc ctctaaacca ccagtacgca gcatggcata aatcaaggca
     5281 cgcatgattt ttagatcact aggcgataag ttagcagaag aaatttgtcc ttctttttta
     5341 gatagcatgg cgttcatatc agattcaaat tgcgaataat ctccaccata cacatctgct
     5401 ataaattcca tcgtttgcat agtaaatgaa atagggaatt ctgcgccttg aataattaca
     5461 acagcagaat tttttaagtc ttcaacgtga attccataat cagataaccg agccattaac
     5521 ccgcacctcc aactttagcc aatgttttcc actgttcttc atcgtataca ggttgagcaa
     5581 tgaatttatt aaataattct agagaagctc cgtctctatt tgaatcaaaa cttgcataca
     5641 tcacattatt gtatttcagt ccgatggaaa ccagatttgc agttacatcg tcaatttttg
     5701 tttcttcctc tgcagtggca tactcttcgt caatcacatt ggatagttgt gtattaggat
     5761 accaaactgc ttttttaccg ccattttcaa tatttccaat aaatccaaag gcgaagtacg
     5821 gaaattctcg tgcggtattt ttgctaaacg ttactccgct tttagcaact aatcctttga
     5881 tttcatccat cacttctatc ggaataccca cgtgatccaa cgcaagttca tgttttgttt
     5941 ctcgcgacac acgacgaaac attttgctgg atgcccattt ttctaatgct gttccattcc
     6001 ctttgacacc gatttttgtg gcaattggca atcgtaaaac ttcactataa gtcggtgcag
     6061 ttccaacaga atctggcgtt gccatcatcg caattaaaat gtcgtccaat ccttcaaaat
     6121 aaaaagtatc ttgttttccc aattaaaatc acccttccca taaatcaagt atttttctag
     6181 tcataatttc ttcaattttt tctttgtttt gttcatacgt accacttgca aaatgttggg
     6241 cacgttgatt tgttgtgcca ttttcggcaa agcgccagta aaaggccgtc tcttcaaatt
     6301 gaactttcac ttgatcgttt tcaactacta cttttatttg atcgcgcata tgtttctttt
     6361 ttaagagcga cttaggaata ttaggaagca actgctttag ataaaagtta gctgcttctt
     6421 ctaaagattc caacgataat tttttcggat caacttttga aaggtttcct aaataatccg
     6481 atatttcaga aaaaccattc ttattacttg gcattttcca cacacctcac atatgtgtaa
     6541 tagttggtta cggtatcgtc gttctcatca ccttgtatac ctgtaaaatc tgaatatgga
     6601 atgccagcat tttgcagcgc ttgttcaata acaactaaat cctgttctgt tccaagtgta
     6661 aaaaaagaga cttggtaata aggtaatttt ttatagactt taccggatgc catttttttg
     6721 ctgttactca catttgagta cacaatgtat ggatacatcg tccctaatct ggctttgtct
     6781 ctgaacactg ctaactttgt tgatttcagc gctgttttca attcatcaaa gctaatcgac
     6841 ataagctaaa ctcaactcca tttctcttgc atcgggattc gtataaatgc gagtaatgtt
     6901 atacgttaca gaatcaattt tgagcgcact taatttctct gtgatggatt tatcccatct
     6961 gactttaatt cgtctgacaa cgtctgtctt ggcttgctgt gataaatatt tttcttgaga
     7021 agtcacaccg agttcttcat agaatattag acgcttaaat tcgtaaattg tagttggacg
     7081 atcgtttccg tctgttcctg ttttgatgtc tagcaattcg gctttccatc tgagattatt
     7141 agtctgtctc ttcggcattt tgaatcgctc cttgcacgat aaatggcgtc atggcattca
     7201 tagctttgtc gagttcatcc tctgaaactc tatattcata ggcaatgccg gcaaccatca
     7261 aaataagata ttcttgttgg ccaccagttg ctgttttgac ataatctttt gccatattta
     7321 aataaaaaga gagcaaagaa tcatccatgc cctcttcaaa atgaatatgt gatttgaatt
     7381 tttcctctaa agacaattct ttagtttgct cttccatctt aaccaccaac tggttttgta
     7441 atttcgtagc gatatactgc cggttcaaat ggagaataaa ccaattgacc atctagcaag
     7501 ttgtaaattt ggaatccaat ttgattttta ccagagaatt tttcaacaag tttttgaatt
     7561 tccaaggcac caataacttc ttgaatttta aatgcagaaa aatcgccaaa atataaaact
     7621 ggcgtgtctg gttcaccctt tttatctgct gcatctgtcc aatccacagg atagccaact
     7681 aattggtaac caattccacc ttctgcttgt gtgaatggcc gcaacaaagg aaatccatca
     7741 tctgttttca ttttttcaat ggcagtcaaa gcagctcgat taataataaa gcgtcccttt
     7801 ttcatcactt ctgtcactgg tgtattttta aattcgatta atgcatcata taatttttgc
     7861 ccagcacctg aagcagttag atctaaaggt tttttaaatg ctacagcctt tttggctaat
     7921 gcaccaggat tttcatttcc agcgtcatca ccattgaaca tataattgat ttctttacgc
     7981 acataagctt ttttcaattc ttccacaaca atatcttcaa ctggaacacc agacattttt
     8041 aataattttt tagttactgt tgccaaagca tcgaattcgg caggattaag caaaatttca
     8101 tcaaactgaa tagctgtttc agcaatatca gttgaacgct ctttcttgtt tacattcgca
     8161 tctgctttct tcacaagaat tggatatttg acatctcctg atgttcgcac cactgttccg
     8221 tatttacgaa gtaaattttc ttcttgagca taagtaataa cttcagatgc aattacttct
     8281 gggacagtaa ctgaaccgtt gccagcttca atccctaaag ctcgagcttc tgcttcagaa
     8341 atatttccag ctacaaaatt agcaaatgct tttcgtagtt gttgatcttt ttgtttcttg
     8401 gtcatttttg cacgtgcctc caatccgttc ttaattgatc caagtaatcc atctcgttgc
     8461 tgttgagtaa tcattccaga acgattttct gaactatctt cactgtctga atcttcttct
     8521 tgatcgttgt cttcaccaga acggctttcg tctgaatccg tactattcga ttgatcatct
     8581 gtattgtcgt ctgtttcatc agttccagaa tctgcgccta attcgtcttt aattccgttc
     8641 aattcatcaa taacactgtc aatttcttct ttcacggctt ctaaatctgc ttcgcgtaat
     8701 tctcctgatt caactttttc acgtaattca gtcaatcgtt gctcacgacg tgccttcatt
     8761 tttttcaata attctttatc catgtatttt tcctcctacg cttctagcgc ttgattaatt
     8821 tttttgatta atttttttct ttcttcgacg tttttttcta attcatcacg acttcttaat
     8881 gcagcttctg tatcttcgta tgctggcaaa ggcacaatag aaacctcgta taattccact
     8941 tcatggattg ttcgaagcat tggttcagaa ttgtagtccc acgtttcttc agtcggcaca
     9001 aaaccaaaac tacattggtt gatgtctcct cgttccatag atttaactaa gtcccttgct
     9061 accgttgtgt ccggtaaatc aacttcgaat ttcaaaccac gttcatcttc ttcaaggcga
     9121 agggttccgc ttttagtacg tcctagcaca ttggaccaat cgtgattgaa caaacaacga
     9181 acatccgaat tactaattgc ctttgagaat gcaccaggaa caatcacttc gcttaagtca
     9241 tcccataaaa gtgtttgact gttgaataca gcagcgtagc cgctaatggt cctcgtatga
     9301 gtttcttcat cggaacgtgt tgaaagattg gtgatgtcaa tcgtgcgaat ttccttcttc
     9361 ttcatttcca tcacctcctt tcaagtcttg atcattcgtt ggtaaggaat catctgttgc
     9421 atttttctgg ccaatcctag ataagtcatt tgaaatatag atagcttgtg tttctggagt
     9481 attctgttta ggaaaaccaa gcatttctgc cacgttatct ggacttgtaa tcccggtacg
     9541 aacgatgttg tagccaatat ttgtttttgt tgagtaagga acaaaatcca aaatattaat
     9601 tttccattcc actctatagc cagaattagg cataaaaaaa agagcggtgt aatgttcgct
     9661 cttgtttttc aatattggtt tgattgcttt gttgtgcaga tacatcatcg ctttttcaat
     9721 atctgtcttc atcagcgatt gatacgtatc aacattgatt cctagaaatt ttcctaagtc
     9781 ttttttgtaa acacctaaat aattcaaaat tgccgcgtca tcaacaggac tttttaatgt
     9841 ctcgatggaa tatccttttc ccaaaggaat catcttaaca gaatgatcac tctcattttg
     9901 cgttccttcc agttgatcca atatagcttt gacaattttt gtttgggcgc tattatttgg
     9961 attgatgtgg gcgtctagtt taagcatgaa cgcaagtaaa ccgcctttag tatatttgtc
    10021 cgtcaaaact ttttcagcgc tcagaacgcc ttctagagtg tttcttgcaa gatcaattat
    10081 tccagcacct tttaatgaat cagttccgat gttcttaatg tgtcgaatca tttgaccagg
    10141 tattttttga ccattcattt caaattcttc ttgaagtcgt tcattgattt tagttgttac
    10201 accgtacgcc aaatgaagct gatccctatc cgttaatggg aatgtctcac cattgatcaa
    10261 taaagtattt gtttccaatt tggtaaattc gaatcctgtc aaataatcat tgggactctt
    10321 caaaatattt agtaagtgat gatctttgac ttcgttgccg tcgggaccaa taacaattgg
    10381 tgtggccaat gccacctgat tagatatgtc ttgaactaat tcataaacat cagaagattc
    10441 catgatagat gaatccgtca catatctttg accataacgt gtgtagtgtc caaaaatatc
    10501 ctcgatatat ccacgttttt ccataaaaga atagactgca ttcgataacc gatcacgtaa
    10561 tttcaatttt tttcaccgcc tttctattta tctatagatg gaacttaggt aatcatctaa
    10621 ctcatctgaa tcaatatctg tcatttgatt catcgtttcc ttatgaccac acaaaaacgc
    10681 cacgaaccca tcaatctttt tctttgattg acgtttactt ggcgcttttt gtccattgat
    10741 gttagtaatt gctacaacgt tcaaagtgca ataaaggaat aatggattat caaattgaat
    10801 tcgtttctca taaaacaacc gttcgacatc gtcaaaagga gcattcaata ctttaggata
    10861 ttgagcaact tcaacgcatt ccaatcctaa gttttctaat ttctcaacaa gtttgtcact
    10921 catcgctgga tcataattca cttgctgaat atcatataaa ttcatgcagt cctctatgta
    10981 ctgcaaaatc tgatcttgat caatcatttt tccgtcgcaa aattcaacga aaccttgttc
    11041 agccaaatca ctgtaaggaa cattatcttc tttttctcga aactctaaat cttcattcgg
    11101 aataaaataa agttgcttta ctttaagaac cgctttacca tcttcatccc atgttgggaa
    11161 gtttaaagat acacaggtca aatctcgtgt acgtgataaa tctaaaccga tataacatgg
    11221 ttcgccactt aaatcaccaa gttcttgtgt ggtaactaaa catggctcta cttgatcctg
    11281 ttcaaagaaa ttatccgcgc cgttcacaaa tacatccaag tgtttcgtta gaaattcagc
    11341 tttggaatga gctgaccgtt gcgctgtttt aaatgccgat tctaaagcag ataaatcgac
    11401 tgatattccc cagttaggat tgcacatttc ccagactttt ctatccgtcc aatcataatt
    11461 tttatttggc tcatagatta gaacaaagtt tgaatcatta tcatcacgtt tcaacacttc
    11521 ttttgcttcg cgatatacac gcattccaac agacgacgaa cctttcccag cagttgagat
    11581 attaaacatc aatggctgtg gtaaagatat ctgagcagac ttaaaattgt catactgttc
    11641 cattttctcc tgtttatgca attcatcgtt caatacaaaa tatggattgg atccctcgat
    11701 attatcaata ttcttggttt gaacaataaa tttatttgag taagccatat cttcatgaat
    11761 atagtcataa gtaatactag aaacagttcc ctttggcccc ctaaatattt tagttccttc
    11821 aagtaggatt ggattgttta agattgtaac tgcaaaaggt ttggcagcat attgcgcttg
    11881 agcaaaatca gatgcgcaag catagcaatc aactgataac gccccttctc cgtacattgc
    11941 atatcctaat gaaccgacag caatcaatgt tttaccattc ttttttggaa tctggacata
    12001 agcctcacga gtaacgcgta caatttggcc tttttcattt tctttaaccc aaccataaat
    12061 ccaagaataa ataaattttt cccagggttc taaaagaaac ggttttccca ccatttctcc
    12121 ttttgtgtga acaataaatg attctaccca atccatcatt tcattcgcgc gatctacatc
    12181 aaaccaaata tcttttcttt ttttccactg ataccagcga tctattgcta aacgaacggt
    12241 cttaggatac ttcttaggtt tctttcttac ttcttttgca aataaatcag cataatttac
    12301 accaggttca atcatttttc agcacctgcc ttcttacgcc atttatttct gtgcgctgct
    12361 agttcatcta ctggctttac ttctgggcgt gtaatttctt catcttttct tgcggtcgat
    12421 cctccagcaa ttagtctacc agtttttgct ttgtttgtta atcctaataa atctagagct
    12481 ttcatcttct tatctgccca agtttctact tgttgtgcca atggatgttt tgattgatta
    12541 gtagctcctg atttattcgt gaatttttgc gtctccggaa atcctttttc cttccacaat
    12601 aaatatttgt gttggtaaat ttcaaaaata tccaagtatg attcgattaa tggatcaaga
    12661 gtgatggtgt acaaatcaga caaattcatt atttctaaga tacgagcttt ttcagcactt
    12721 actttttcat caataatcgc tttacgttgc gctttagtcg tcatttttgt atacaccccc
    12781 ctttgttttt tgaaaaattt gacctaacga tgcgcgtgac tccccgctac cctatctccc
    12841 cacgcgaaaa aattttgaaa atgaataggg gggcttcaat tgaaatacga aggaaaaact
    12901 tttttgtctt ccgtttcgtt ttcaacaatt ggatgacatt ttgaacataa tagcatgaga
    12961 ttgtttggat caagcttaag cagttcgttg tctttgattg gtacaatgtg atggacgtgt
    13021 gctctctttc caaatatgaa ctgaccacac cgctgacaat gaccaccttc tctttcataa
    13081 ataaattgac gcatatcttt ccatgcttgc gttcgataga atggtttgtt ctcatgatga
    13141 taaacagact ttgcttgctg cttcttcttg cgtgatctgc ttgatctctt gtgttcagta
    13201 cagtagatac cctttgctat tttgttcgtg catccgtcaa actgacagta tttcattcta
    13261 cttcacgaat aagatttatg atatcgcctt ttgcacggat agcaccagga atatcaatac
    13321 catgtttctt agcatatgca cgcaattctt ttgcagtcat attgtccagt tcatccgtat
    13381 cttcagtgga ctgatcatta gtagcttcgt ctccatcaaa attagcagca gtgtttccat
    13441 cgctatcaag aatttcactg ctatgagcta tgagttcgcc attgacagca acaaatgatt
    13501 tgccatcact caaaattcct ttttcttctt ttacttcaaa atttggttct tgatctttcg
    13561 gaacaactac tgtccttttc ttttctgaat cccaatactc tgttccacta atggatgttc
    13621 taattttgat cattgccatt ttaattctct cctttgtaat ttgtacggat tacttttgcg
    13681 cccattcgct cataccattc aatcgtttcc tgcagattgg gtagagtgtg tgacaataaa
    13741 gaaataacta acgtattcat tccagttctg taatcatcat tctccacttg cacatgtgca
    13801 tgattaccat tccaattttc tctgtacgga ctattctctg cttcaattgc tttcttataa
    13861 actttagcca aacttgattc tacttcaata ctaagtaccg cttcatgaaa atcattcatt
    13921 tgtttactct cctatcagat attttgtggt atgatttata aaaaagatgc gaggtgtttt
    13981 aatgcttcaa gaagttaata ttaagtatcg tgatcgtcaa aatacagaga caatttattt
    14041 gccaaatagt tgtccgcatt gcggaaatat aatgactccg tatgtgtatt ttggggtttc
    14101 atcatatagt gcgttcgata atgatagaat ctttggcgta ttagtacagt gtataaatga
    14161 tgagtgcaac aaattttata gtttacaatt tataaatacg tttaatgaag atgaaggata
    14221 ttctgaaaat attttaatta aagattatta ccgtccgccg attaaaatag atttaccaga
    14281 aaatattgaa aaagtttctc ctacatttgt tgatatttat actcaagcca cacaagctaa
    14341 atctgagcaa ttggaacaaa ttgctggagt tggttataga aaagctctag aatttctaat
    14401 taaagattat gcaatatcta aaaattcaga ggatgaagat aaaataaaaa aaatgttatt
    14461 aggtaaaaca attgaaaatt atttgaatga ttttcctaaa ctccaaagat tagccaaagc
    14521 tgctacttgg attggtaatg atgaaacgca ctatgttcgt agacatacta ataaagatgt
    14581 tcatgatatg gatgcattta ttaaatcagc agcgcaattt atcgcggctg attatgatgc
    14641 tgatttagct gatcaattta taaatggaaa taactttatg ccaactacta attaaaagaa
    14701 taaaagagcc acagatacca attaataaaa cggttgggat atcttggctc tttcatattt
    14761 ttttgacact aatagaatat catgctgcta acaatgttta cgaacattta gtgaacagtg
    14821 taattttaga ataaagagag ttgttctttg tacttctcgc tattgtaaaa atcaatttcg
    14881 ttttcttttt tcaatcggtc agcttgtctg tgttcatact catctaaaaa gttcaatgtc
    14941 tttctaattt ctgcatgtcg ctgtctgaca tacgatgagc tatacccaac agattcagca
    15001 atatcttcta agctcatttg atcaacatat ttcatcttaa caatttgatt atcgatacca
    15061 gaaaagctat ctatcagcaa taacatttct tctttttgct cgatcaacaa ttccaattca
    15121 ttttctatct tttggatatt ctcttctaaa gaagaagttc tcgaattctt ttcgatgcgg
    15181 acgtttgcta aatcaccaca gacccatcga ttcaattcaa gcttactttt attaagattc
    15241 cactttaagt aaagaatctg ctcatctagt tcttggtaat cttttaacca atgaaatctc
    15301 acaaacgcca ccccttatgg tagaatagta ttgcgctgct atctcgaaag gggtagcttt
    15361 tttatttttt cttagccgtt ccattctgga ttgccttttg ttccaaacgt cgttttttct
    15421 tcttaatctt tgattttgtt tttcccatac aattctcctt tccgaaaaca cattttgttc
    15481 gatttttaat actaaataaa agttggccag aatttttcgt tcgtctttac tcatcttttt
    15541 ttgaataaga ttactagcat aaaaatagtt agtaccacga aaatgaacag gtaaatcaat
    15601 tacgttcacc ttcttcatgc cagttatcag ccactagaat ctttgcgtta gtgccgccac
    15661 cgttttcttt tttgtatttt tcaaatgctt tataagcagc tgtttttgat ttatagattt
    15721 ttccggaacc gactgggaac atccatgtgc cctctgtgat agctaagatg tatactttca
    15781 tttaattcac ccctttaatc cccaacacta catatccgga cttctgcatg taatcagtaa
    15841 tataagtgat ctctgctttg tatgagtcac cagtgtaact aaatgttggt ctcgttagtc
    15901 caggtaattc atgtttaatg tattttttat attcacgtag tattaactga tcaccaactt
    15961 tgaagtcacg atcattcttg cggatctcga attgtttgcg tccgctcgtc actgcttcaa
    16021 aatattctgg tagtattttt agttcgtgga tcattctgtt tcctccttca aaattcgctc
    16081 atcatcaaaa agtgaatctc cacaaaatcc ttctcccaaa tatttatcgt tcatgtataa
    16141 gtcaaccaaa tatttgtgat ctgcttctcg atattcatta atattaacga tctttatttt
    16201 gtaaacatta ccttcgtcgc tcactatctt gaattgttct ctgactttca ccaccgttcc
    16261 tcctcttttt gaaaaaattt tagtgttttt gtaagacccc ctaaatcagc ttttttattt
    16321 gatataatac ttgtatcgat taatcaaatc gaaattaaat ttaaaaaggt gatttatcat
    16381 gaatatcaac aatttagtaa aactcgttga acaacgtttt aaagaatcag gattagaaca
    16441 attaaaaaaa gataattctc cagaattatt ttctaatttt accgaagacg acattcctac
    16501 tcttaaacaa ttaaaatatt attctgatta ttggacgcaa actcgtgaga atgaattaaa
    16561 tttagaaaaa ctcattatta aaaagaaata gcaactccag cccacacggc tggtttttta
    16621 gtttcaactt ccctccttac gggtcaaaat catcggttcc ggcataatct gatcatcctt
    16681 tagcaacaaa tcaggatatt ttttccttag cttcttattg ttcgtgtaag ttttcaatag
    16741 ccattggttg gcttcgcttt tgctttctcc agaaaaataa attttccctg tttggatgcc
    16801 tgcaagttga atcatcattt tcacctcaaa tcaaattttt tctgctagat ggagcaaaag
    16861 caatccgaca tcaagaatct tttgacccat tatttcagct actttgctcg gcttaactcc
    16921 ttgagaaaag agcaagcgtg cttgctctat ctcttcgtga gtccaaataa atttggcttc
    16981 ctcaaggata attactggat cagttgctct cattgttcct ctggaaaaat gatgtcattc
    17041 ttatgcttgg accaggtatc tgcatatgga acaaagtatt gtctagctaa ttcgatttga
    17101 tcaattaaag cagtttctga taattcatgg tctgcagcaa tttcattcat actatcgcct
    17161 tcatctaaac agataagacc gttgacgtta tcgattttta catttgtgac tgcttgactt
    17221 tctccacgct cataatattc tacagttgat actgatacat cagcttcgga gtttaattgc
    17281 agaatctgtt actgccggga taatttaata ttcttttcat aattcacctt ttttcctact
    17341 gacatcgaat atttttcatg ttatgattag ccgtgaaagg agagctatat tatgtgttta
    17401 cacatgatgt ccttagctca ttcggttctt tggaagcttt tttggaaata actctcaggt
    17461 tcttgacgca aatctcattt attctttgaa acaaaaaact tccattttcc gagagctttg
    17521 gatcacttca aaagagattt gcatttatat ttctgtgacc ttgagtcgcg taaagaacct
    17581 tacagcaatg gcggcaattc cactttcagg aaaccgcctt ttttcattga tattggtggt
    17641 tagcggacta cccattttct ttcggcagtg ccaaaccaca aatgatctgt gttgatgttt
    17701 aggtagttgt ataccagata agctaaatcc aaagtgcctg catcttcaag acgacaatat
    17761 tccaaaaatt ctcccatagc tggacataaa aatcctggag ctatcatatc ccaacagtta
    17821 atagtgcaaa gaaattcgtt tagcttctga acgttttctt tggtttcacg aattacaaca
    17881 tactccaatt cttctccgct ggcagagcat tccaaaactt caaattgatt aagaaatggt
    17941 agaacatatg cttcaaacat caacattcca tctttctgtt tttgcacttg ttttgcatca
    18001 atcatgtcta tcctcctatt cagttatttc ttccattaat ggaattattt caggtcactg
    18061 gacttaacga aaacaccatt gaccatcttt ccagtccgac ctttgatttc gtcataagca
    18121 aaatccaagc actcgtataa atccatatca ttttgcattg ccaagatgat caaggttact
    18181 actacatcgc caattccgtc tcttaaacca tgttcatctt ttctagctag agaagcggca
    18241 acttccccga tttcttcgac cgtttttaac atttgcttgc tgaaatccgc ttgatccaat
    18301 cccttatctt ttgcccactg ctctactttc gcgattagtt cgtccattat ttctcctcca
    18361 catacctaaa ctgtcttcct tttgaatcaa tccataagct cctagctcta tcccaaataa
    18421 tatttttgct cagaccagta atttcagata actgtttagc ggtacccgtt actagaattc
    18481 gatcaccatg ccagattgca atctttctcg gcgttctccg tttgggcttt tcagtccaca
    18541 ttgatttacc gagcttttgg acttctgcaa ctattttttt atcttcctgc caatcttctg
    18601 aataagtcaa ttcgataatt cgctgcattg ccgctttctt atccatcccg acattctcct
    18661 ttcagtaatt tgagtacttg atcaagtgcg ctctcacgtc cgccatgaaa tgtgttgagc
    18721 cacttgtctt cgtacgacac actttgtctt aaagcttctt gatgcattaa ttcgatctgt
    18781 gctgtaaatg tctttaggtc catcttgttt acacctgctc aagttcatta agatgttttt
    18841 gcaagccttt aacacaatca atgaatagta atttggtata tgctaaattt cttaattgcg
    18901 ttccatcgat atagagtgcg aaatagtatc tgagtttact ccaactcgaa cgatcattct
    18961 taattccttc aattccagct tcttcgagtt gatcatatac gtctctcagg acgtctaatt
    19021 cctcgccctt tttataattc gctatttcat taattagttc tagataatcg atcttcattt
    19081 ttccacctct tagaatggtg cttttgattg tctattagct cgttctagcg cttttttctt
    19141 gagataggct tcttggtcga ttgcccattc aggaagcttc tctcgtcttc ctgtgcgctt
    19201 gtatccactg cttgcgttct taggttcact tttttctttc cttgcccaac ttcgaatagt
    19261 tgccaaatag tttttataag tcttaccaga tgattcacaa tactctgaca gtcgttcgat
    19321 tcgctcttgg taatcattag ggaattctgt tttgagtttc tccatctgct catctgacaa
    19381 aagaacattt ttatactctc cgtatttatg acggacgggc ttagccttcg attttttcga
    19441 aggcggtaac tctcttatat attcttttgt attattaaat gtattattaa tagatgtatt
    19501 attatctttg actttttcgt caataggggt attgcgtttt tcgtcaatag ggtattgatt
    19561 aattcgtagg tacctattga ttatttgatt ggtaccctct ttgtaaatga tttcccgatt
    19621 caagtatcca aacttaatca aatcacttac ccatcgcgat atggtctctt tattcacacc
    19681 atataaatct gcaaagtact cattgcctgc ccaacaaaag cctctttcat tacacaaggc
    19741 cgttatctct ccgtataaca acttagtatt tggtttaagt cttttgtcgt accttacgtt
    19801 ggctggtata atcgcataat aacttcgatg ttctgtcatt tttcccctcc aatatttaac
    19861 tttttgattg ttttctggtt taatttgatc cctttgattt gatatttatt tttgaaatta
    19921 atcacaccta ttttgtgctt ctctgtgtga tggattctgc agagtgctgc aaatgtgtac
    19981 tctgcatgat caacttcttt gcgctttcgt cttcctagcg ctttgtcaaa gtgatcgatg
    20041 tcagctcctg ttttgccaca gatgcagcag actctttttg tgatgcattt gtagaagtaa
    20101 tattcttgat tcgcaggtaa aatctcatag ccttctttga aaggaatatg atgttcaaag
    20161 atgaaatcta agatgatatt tgctaagaca ttggcatcac tcacagttgt attcgattcg
    20221 tctttcaggc ttattttgcg ccctgtgaca ccttcaaaac gaaagtagaa gaattccttc
    20281 cagaagtccg ttggcatgcc tgtatcgata aaaatatcgc ctatcagcgc atagatgaag
    20341 tttcgttgct gtacggtgaa acgtctagga tcaataaaac gaatttcaat gactcgatca
    20401 ccatcgtagc cgtcatacat cgtctttagt cgatcaatgt tcacttcctc attgatggtt
    20461 gcgcttatgt cttttccttt gaactttttc agaaccgctg aatatgaatc gattaatggt
    20521 ttaaacactc atatcacttc ttatctaatt cttttctctt agctgctatt gctcgctcca
    20581 tcaaggcaca ttgctcatag cttaactgtt caatagtttc aacgttatca gctaagagcc
    20641 ctaatttatc tgtctgctca ttaacatatt cgattaaggt tttggtcata tctttaccca
    20701 tctgctcatt gaaagcttct agaatcgtct ctagcatgtt taatttcttt gtatcgattc
    20761 taggtggtgt tggaatatct tccccttgaa atacatataa tcccagtccg tgtagagcca
    20821 atgctttcac aaagcatcgc ttcaatgagt tattgatttg cattgcattt ggtttaacaa
    20881 ctggttggtt tcgataatct aaaacaggaa ataattcggt ttccgtgtgt cctttaaccg
    20941 ttactgagac agatacataa gtcccagttt catccataag aaaaggttta tattcctcaa
    21001 caagaaagtc ttgatgagtt ccagaaacaa ccctgtagtg tttgtactca ttaatagtta
    21061 ccgttgcctg tggatcattc tttttcataa tctcccacgc gtgagcccaa gataaataat
    21121 caaaatttcc ttttttcttg agaattttat ttaacttacg actaaaaagt ttttcaaagt
    21181 tcgttgtccc tttgatttca ctcatcaaat tctgcctcca tttcagcaat gtatttctta
    21241 cctggtccgt aataagagat atcaatcaag ttatctctgt cgtactcttc tagcgcatca
    21301 atcaagccat cttcgatgac gtaaatatat tcaggtttat tcgaatgctt cgatagatgg
    21361 ataagataaa catgatccca aatagttaca tggttgccta aatcatcttg atcccaagct
    21421 agttcttcat tcgtcaaaag atttcgtctg atttttcgac cacttgtttc ctcaattttc
    21481 ggcttgcccc aatcaggatc aatcaaatat tgatctagag tggaaagttc tttttccata
    21541 tgctaaaatc tccttatgat gtgttttctt tgtgactctt tgcttgccgg cggagtcact
    21601 tttttatttg ttgccatgct ttttgcttgt caatatgttg ttggcttagg atgattggtt
    21661 tatagtattt ccaccagcaa ttagcaattg ccgtccctat tcttagcgct tcagctctat
    21721 tcattgtcat caccaaaaag tctctgttgt ctgttcagtt gatcaatttc catacggatc
    21781 gcagtttctg gcaaccacat ttcaataaat gaaacagcat catcgaatct cttacgaggt
    21841 aactcgccat atcttgggat tgaaaaggta cgtttaaatt cagaccaaaa ttttgagaat
    21901 acttttttgc tgatttcttc ataagctcgg ctttcttttc cccctagaac tcccataact
    21961 ttcatatttc ctttttgctt aatttcaaac tcttgttgtc cgctaattcg catagtatct
    22021 ttaagcatgg aaacatcttt tttaacatct ttcatttctt ctaattgata gatcatcata
    22081 tcttcaattg tttgaggaac agtattcttg cgaataacat cttccatttc gttgaatgct
    22141 tcaatgtatt tttgtttgaa gtagatagct ttctttcctg taaaccccat agccagcaag
    22201 aaaaatccat ctctactaat gaagaaaact cgtcgatttc tgccatatga atctggttca
    22261 ttaccttcta caaacatctg tccaaaattg gacacatctt tttttagtgc atcaatatct
    22321 cttaaaacat gttgatgttt tttctcgaag ctttctgcca cttgtaagct cgtagtcaca
    22381 gcttctttat ttttcaaaat tactaattct tgcattattt cttctctcct ttttggtata
    22441 atttagttaa aaactggtgg tgtttatttt gaattttttt cacttaacaa tttttcaatg
    22501 gatgtcaata ataagtttta tattgagtac gtttttaaca gggataaaaa taaaaggaat
    22561 gcgccctcaa ctagaagttg aacttaatgc ttcttacttc gctgcagata tgatttatac
    22621 aaaagtaatc atttctaact attcaacaga accagctatg ttagttaatt tagaactttc
    22681 atccgctaac ttaaaccgca gatggtcagc tactccattt aagaaattaa ttgctaaagg
    22741 aggatcagtc gatgataata gaatatactc tgaagctgta ccgctaaata ttcctcctaa
    22801 aagtgctata tcttgttacc ttgcatttga agtagggaaa attaatttta ataaactcct
    22861 taatcaccaa acaaaaatga tatttactct aaatagaacc caaatccaca aaattgttga
    22921 tatcaaaaat acaaacttcc caatagagaa attagtgaaa gaattaaact gagtaatagt
    22981 aatatacatt ctagtttctc ttttctattc atttttagtc agccccctcg gttggctttt
    23041 tcgctctgta ctcagcttca gcttcatcaa gccccataaa aatccaaacc atgtaaacaa
    23101 tcgttcctat caacgcttgc ctgcttcccc aaagtcctaa agcgtagatg attagcggtg
    23161 cgctgaacac tagcgctcta ttaaattttc ccatccgctt gcctccttaa gatttccgaa
    23221 aaatttgttt ctaaaaattc aagtgtttta cttcttaaaa atagatatgt gtctcttcct
    23281 tcaactggat aatagacaaa tccatttttg tttttctcga tatcgataat atttctatat
    23341 cttgggttct ttaaaactct agaagtaaac caatcatatt ttctgttaat ccgttctagc
    23401 acttctggca acgtcatcca tctaccagta tcatcagctt tttttaactc ctcataatcc
    23461 acttgggaga taattacata gccttctgga attgggattt ttgcttctag atattgcatt
    23521 agctgttcct ccct
//
LOCUS       DADVXU010000039        23208 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.041, whole genome shotgun sequence.
ACCESSION   DADVXU010000039 DADVXU010000000
VERSION     DADVXU010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 23208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.041"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            438..992
                     /locus_tag="KB953_001431"
     CDS             438..992
                     /locus_tag="KB953_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBB6866415.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(1165..1638)
                     /locus_tag="KB953_001432"
     CDS             complement(1165..1638)
                     /locus_tag="KB953_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBB6866416.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1650..2186
                     /locus_tag="KB953_001433"
     CDS             1650..2186
                     /locus_tag="KB953_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBB6866417.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            2226..2597
                     /locus_tag="KB953_001434"
     CDS             2226..2597
                     /locus_tag="KB953_001434"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBB6866418.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            2881..4152
                     /locus_tag="KB953_001435"
     CDS             2881..4152
                     /locus_tag="KB953_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBB6866419.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            4397..5422
                     /locus_tag="KB953_001436"
     CDS             4397..5422
                     /locus_tag="KB953_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBB6866420.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5716..6825
                     /locus_tag="KB953_001437"
     CDS             5716..6825
                     /locus_tag="KB953_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBB6866421.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            6908..7804
                     /locus_tag="KB953_001438"
                     /pseudo
     CDS             6908..7804
                     /locus_tag="KB953_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            7820..8644
                     /locus_tag="KB953_001439"
     CDS             7820..8644
                     /locus_tag="KB953_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBB6866422.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8676..9986
                     /locus_tag="KB953_001440"
     CDS             8676..9986
                     /locus_tag="KB953_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HBB6866423.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            10134..11393
                     /locus_tag="KB953_001441"
     CDS             10134..11393
                     /locus_tag="KB953_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBB6866424.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            11403..12275
                     /locus_tag="KB953_001442"
     CDS             11403..12275
                     /locus_tag="KB953_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBB6866425.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            12287..13117
                     /locus_tag="KB953_001443"
     CDS             12287..13117
                     /locus_tag="KB953_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBB6866426.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            13157..15340
                     /locus_tag="KB953_001444"
     CDS             13157..15340
                     /locus_tag="KB953_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBB6866427.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            15401..16621
                     /locus_tag="KB953_001445"
     CDS             15401..16621
                     /locus_tag="KB953_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HBB6866428.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16618..18078
                     /gene="gtfA"
                     /locus_tag="KB953_001446"
     CDS             16618..18078
                     /gene="gtfA"
                     /locus_tag="KB953_001446"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HBB6866429.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            18155..19261
                     /locus_tag="KB953_001447"
                     /pseudo
     CDS             18155..19261
                     /locus_tag="KB953_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19583..19792
                     /locus_tag="KB953_001448"
     CDS             19583..19792
                     /locus_tag="KB953_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HBB6866430.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMASKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            19785..19934
                     /locus_tag="KB953_001449"
     CDS             19785..19934
                     /locus_tag="KB953_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBB6866431.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            20088..20753
                     /locus_tag="KB953_001450"
     CDS             20088..20753
                     /locus_tag="KB953_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBB6866432.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(21037..22122)
                     /gene="xerS"
                     /locus_tag="KB953_001451"
     CDS             complement(21037..22122)
                     /gene="xerS"
                     /locus_tag="KB953_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HBB6866433.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            22456..22689
                     /locus_tag="KB953_001452"
     CDS             22456..22689
                     /locus_tag="KB953_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HBB6866434.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            22686..23093
                     /locus_tag="KB953_001453"
     CDS             22686..23093
                     /locus_tag="KB953_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HBB6866435.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
BASE COUNT         7946 a         3466 c         4428 g         7368 t
ORIGIN      
        1 aattattgac gctcccgaca aaattatgat cttgcgtaaa gttataaaat attaataaaa
       61 atattttatt aatgaaaagc ttggtagcac ggttattcat atgctaattt atttcacttt
      121 atagtaaggg gagttcttag tatattgttc aaacttaaat ttcatataat ttaattatgt
      181 aaattagcta ttctaagaac ttctacggat aactgcccgt ctaaaattta aataagagct
      241 tagaatcttc atataagtaa ggttaatttt tttgtacaaa aaagtcggaa tattctgagt
      301 tactgaaatg atataaaatc ataaactaaa tcccttgatt ttattgactt tcaaaagttt
      361 atatatataa gttaatgatt atggtaaaat atatacataa tcattaactc agaatatttt
      421 taagaaggga aacaatagtg agctataatg tacaaccgtt aaggacaaat gaagagatac
      481 aagattttca gttttggcta agaagaacaa gtaatccgga aagagatagt tttttatttt
      541 tgtttggaat caataatggg ttacgtatgt cagatattat aggattaaaa gttggagata
      601 tacgaggcaa aagtaagcct attattgttg aacataaaac aggtaagcga aaaccgattt
      661 ttatagataa tttgagagaa gaaattttat tatatactga aggtaaagaa gaaaatgatt
      721 ggttatttcc gagtcggcaa caaggtcgac atataacaag ggatcgtgtc tatcagatat
      781 atgcagatat agcagaaaaa ttaggacgag atgatatagg aacacataca ttaagaaaaa
      841 cttttgggta tcactactac aagaaaacta gagacattgc taccttaatg tttattttta
      901 atcatagctc gcaagcaatt acaaaaaggt acattggtat aactgaagat gaaataggag
      961 catcacttcg tggatttaaa ctaggtgtat aatttaaaaa tttttataaa cagtaaagac
     1021 aaagattaaa agctattttt atctagaacg attttatttc ataaagttcg cggaataaaa
     1081 gctggggtta ctggtacttg gtataggttc gtgttatata aactaagtaa ttaaataaca
     1141 tttttaggtt ggaaaaggat gaaattaaaa aaagagaatc aatacggtct caaaaacgca
     1201 aacgctgcgg aataagaaaa atactgataa ctcaacgttt agcagactat ctaataggga
     1261 tagtctgctt ttttcgtttt tgaccatact tttgaccttc tgcaaacttc cttgcttttc
     1321 ctctgtagaa ctcatgaaat ctgcaaaacg aattgccgtt tcctctgtct gctttttcgc
     1381 gatatgggtg tatatatcat ccatagtggt tttaatatct gtatgtccaa gtcgaacata
     1441 cacttctttg acagacgcac cagattcaaa gagcagacta caatgggtat gacgaaaacc
     1501 atgaagtgtg atatgtttta agttattctc tttaatgatg attttcaaat tgtgatttac
     1561 atagtctaaa taaaggggct tattttcttc tgatgaaaac aaaagttgtt caccatcttt
     1621 tataggtttg attctcattt ttagatatta tgcccgcacg tattcatcac atttctattg
     1681 tgaatcgctt tattcgaccg acatttgact tttatcataa tatactcggt ttaaaattac
     1741 taatgaaaac aattaatcaa gacgatcata ccatgtatca cctgtttttt tcagataatc
     1801 accaccgtgt gggaactgaa ctgacttttt ttgaagtaca agaagggaac aaccagtttt
     1861 ttggaaccaa tacgattgaa cgaacgatac taaaagttcc ttccgaagca tcattgcatt
     1921 tttgggaaat atattttgaa acacaaggcg tttgccatta tggaatcgaa tcattttctg
     1981 gacgacctat cttacgtttt gaagggccag atgcaacacg attagcttta gttcccttac
     2041 gcgaatttga agatattgat gattatttcc cgtatgtcac agaccagatc cctacagagc
     2101 atgccatttt aggaattgat gccattcaac tacgcgtaca atattccgat gcgactgaac
     2161 gagagttatt gtcggtaggc tggtagcaca aagaatacgt cagtttcttt tcgggtcatc
     2221 aacgagtgac aatcttagaa aatcaagaca ctcagttcta tcaagaagtt cagattattc
     2281 aagatcgtga gaatccagtt gccattgaag ggattggtag cgtccatcat gttgcgtttg
     2341 gcgtagaaaa taaatctgat ttacaacgca tcgacaaaca attacaagag cgcaatttta
     2401 taaattcagg aataaaagat cgtgaatttt ttgtatcact ttattatcgt gacccaaatc
     2461 aattattgat tgaaattgct acaggggaag gaaacctaga tgcaaaagcg tatgaaaatc
     2521 aaagtccacg ctttgaagaa attcctttat ttttaccgca gtacttaaat ttcattcgtg
     2581 aacaagtgga gcaataatta gctaatcaaa tccatgacga agagtaagag gttgactcat
     2641 aagtcaaaaa ataaagaaaa acgcatcaaa tccattctaa agaatgttga tttgatgcgg
     2701 ttcctatttt tataaactat cattttcgac taataaaact acttatgagt cagcctctct
     2761 ttttttcttg atttttatac ctctttctag gtaaactaaa catctgccaa aaataccgtg
     2821 tctcattgaa acattctcaa ctatttcaaa ataaagggga gtaccaaatg aacgagtata
     2881 ttgaaattat ccacgcaaga gaaacaactt aaaaatgtca gtctcaaaat tcccaaaaat
     2941 aaaattagta tttttaccag cgtatctggt tcaggaaaat catcgattgt ttttgatact
     3001 attacccaag aagctggtcg tcaactgaat gacacctatg atagttttac ccgtttattt
     3061 ttacctaaat acagtcgtcc agaggttgat gagattgata atctttctac ggcgattgtg
     3121 gtcaatcaaa agaagattgg tggcagtgcg cgctcaacgt tagggacgat tactaacatc
     3181 aatgcgttat ttcgtgtgtt gttttcatgt tttgcaacac ctagtttagg atatgcaaat
     3241 gcttattcat ttaatgatcc tagtggaatg tgttctgtct gtcagggaat cggtaagacg
     3301 attgcatttt taataggatt gctttatttt tcggctattg ataatgctga ttttgtattt
     3361 tctgatccat ttaccgcaga agttttgtct ggaattgagg aacaggcaaa gaatcatggt
     3421 tattttgtat tggtgcataa tgttaattca gcagaagatg ttaaaactat tcagagaaat
     3481 tggcgttttg aggggttcat tgttgttggt gtacgtgtaa aagaattttt ggaattaaat
     3541 aacgtttttg atggtcctgt tgtttatatt gatacgcatc tttcaaaaga gacattaaga
     3601 gaacttgaac aacaaatgaa tcgtttattt gtaaatacaa atgattacca agctagtaca
     3661 ttggcagtgg aacatttgat tcattatggt catgagaaaa tagcgtttct ttcttttgac
     3721 tacattgtag gtgaaccaag tgttattcaa gaacgacatc aagcttatat cgatacatta
     3781 aagaaatatc atttaacctt taaatcgcat tggatatata acaactctga atttgagcga
     3841 atttatcaag aattagctga atttactgca attgttgtta ctggagatta tttagcggct
     3901 aaattagtaa aatttttaaa aaataagaat aaacgagtga tggaacaatt atcgattatt
     3961 ggatttgatg atattacttt tgcagaatta atggatcctg cgttgacaac aatacgacta
     4021 aatccaggta acaagggggc gattgctttt caccaactga ttggactggc caaacaagaa
     4081 gaaataaaaa atcaagtact actattagag ggagaattga tttctcgaga ctctgttcac
     4141 aaaatcaaat aaaaaagctc tgtgaaataa gtatagttaa caataaaata tccgaacgat
     4201 agagagaatg aactgtgatc tagcagttcc tcgtcgcaaa ttcactagaa ttagcattat
     4261 ttactctaaa ttgttcggat attttttctt gtttttaatt atgattacag tgcctatttt
     4321 taacgaaatt tgcttatttt caaaatgtat gattatatgt tagtaaacac tttcaaatat
     4381 gaaaagagga gctattatgg taggaattag ggatgttgca aaacgggcgg gtgtgtccat
     4441 tgcaacagta tcgcgcgtgc taaatgagga tccgacactt tcagtaaccg atgaaaccaa
     4501 acgaaagatt gcagaatcgg ttaattttta tcattatgtc aaaaaaagtc ccgttgcaaa
     4561 aaatttaaca aatattgcat taataacgac agtttctgaa gtagatgagt tggaagatcc
     4621 ctattttcga acgattcgca gagggattca agtcgaagca gaacaaagca aagtcaattt
     4681 gaagaaaatt attcgtttgt cagaaacacc gttggagcaa gacgatttgc taaaatgtca
     4741 aggtgtgttg attattgggc aggtgctatc ttctgtgatt gaagaagtat cagcaattaa
     4801 ttcgaatgtc gtagtgattg atgaccctag tattgaagct gaagtagatg cagtttatac
     4861 tgatttaaca aaagcaacga atcagcattt agatcgattg tataaaaaag gacaccgaaa
     4921 cattgcgttt atcggaggag gacgtgttcg tttagatagc caaggaaata aacacgtgtc
     4981 agatgatgat tatcgaaaaa tagcctatga gacgtggatg aaacaaaaag gattagaaaa
     5041 atatagtcaa atttatttag aaggctggac cacacttgac ggaatgcagg cagcagattt
     5101 acttttgaaa aaacatgcta aagataagtt gccgactgct atcatggtag gaaatgatcc
     5161 tattgctgtg ggggtttatc gttcacttca aaaaaatggt ctacatattc cagaagatat
     5221 ttcgattgtg agttttgata atattgaagt tgctgaattt ttaacacctg cattaaatac
     5281 ggtgaatgtg aatacagaag aaattggaaa actagcagtt cgcatggtta atgaacgaat
     5341 aaaagaagtt agaaatgttt cgatcaaagt gattgtttct aataatatta ttgttagaga
     5401 aagtgaaaaa aatatcgatt aaaaaagtaa acatccaaaa aagggtgttt acttttttgt
     5461 ttactaatat ttttagtaaa aaatctcgac aaaacattaa gtaaacagtt atggtgttgt
     5521 taacaaaaag aaaacgcatt caaaacagga ggaattaaga tgaaacattt caaaaaaata
     5581 gctttaacag ggatagctgt tttaactggg acattactcg cagcatgtgg tgggggagat
     5641 agtaattcta aagacgggca agtaacttta agctatgcga gttgagatac tgaccaagct
     5701 ccaggactaa gaaaaatgtt agatgaattt gaaaaacaaa atcctgatat caaagtagaa
     5761 atggagacaa cgccttggga tcaatattgg gttaaattag aagctgcaac aacgggaggt
     5821 aatatgccag atgtagtaac catgcactct agcgaatcct ataaatatat gtcacagaat
     5881 gctttgatgg gacttgatga tgttatcaaa gaaaatgata ttgacatggg aaactttact
     5941 gaaggaattg ctgatttcta tacgcttgaa gacgaactat acgcaatccc taaagatgct
     6001 tctgtagtgg ggctatggta caacaaagaa ttatttgata atgcaggtgt cgattatcca
     6061 gatgaaacat ggacatggga aacactcaga caagctgcaa ttgatttaac agatgaatca
     6121 aaaggcatct atggttttgc atcacatatt ggaacagaga ctggctactg gccatttatt
     6181 tatcaaaatg ggggagaagt atttgccgat gacaatacaa aatcaggttt gaatacccca
     6241 gaagcacaag aagctttagc tttttatcga gatattgcac ttgttgatca tgcttcacca
     6301 acaattacag aattacaaga tcctgataag ttaagtcgtt ttcaatcagg tagattagca
     6361 atgatgattg aaggaaactg gtttgcaccg ggatttttga aaaatgacta tctagttgaa
     6421 aatgttgctt ctgccgtgtt acctaaggga aaagttaatg caacaattac aaatggactt
     6481 ggttgggctg ccagtgcgag tacaaaatac ccagaagaag tgaaaaaact aatggcattt
     6541 ttagctagtg aggacgccaa taaaatccaa gcggaaacgg gggcatcaat cccagcacta
     6601 aaaggctttg aagacgagtg ggctgcttct tcagatacaa ttgatttgag tgcttttgga
     6661 aaaatgttgg agtatggagt ccctcgtcca tacaataaag atggattacg agctgaacaa
     6721 gcagaagttc aaattattaa ccgtattctg tctggtgaag taactgttga agatggaacc
     6781 gctcaaattg aaaaagaagt caatgcgata ttgagcggaa attaaattag ttaattatac
     6841 aatatacagg cgaaaggatt ctatttcgtc tgtatattaa tggatgaata ccaaaaaggg
     6901 ggatattatg aaaaatcgaa tttttggaaa atatcgtaaa gatcaagtga tatggggcta
     6961 tatttttatt tttccaacgg cattaggatt aattttatta aatatatggc cagcaattca
     7021 aacagcaatt ttaagttttc aaaaaacggt agggttcggt gatactgaat gggtcggatt
     7081 aagaaattac caaaaaatgt ttgcagacgg tgaagttttt cagtctttat taaacacatt
     7141 aatttatgca gtggtttcga tacctagtat tgttgtatta tccttgttgg tggcagtttt
     7201 aatgaataga aaagttaaag gattgtcgat ttatcggaca atttattttc taccagtcgt
     7261 tgctgcacca tccgcagtcg caatgatttg gcgctggatg ttcaataatg attatggact
     7321 gattaataac atgttggcta aaataggtct cgatggacca gcatggttaa cggatccatc
     7381 gattgcgatt tattctatta tcattgtagg agtttggagt gctattggtt ataacatggt
     7441 gttattacta gccggcttat aagaaattcc taaagattat tatgaagcag cggaaattga
     7501 tggtgctagt ccaatccagc agtttttcaa cattacgtta ccattagtta caccaacatt
     7561 attttttgta gttgtaacaa cagttatcaa tgctttccaa gtatttgatg ttatttttat
     7621 gatgattact cctaatagta ccgccatgtt taaatcacaa tcattagcct atttattcta
     7681 taaacattca tttattttaa atgacaaggg atatggttcg gcaatcgtta tgttcctttt
     7741 agtgattata ttaattatta ctgcaattca aatgaagttg cagaaaaagt gggtaaacta
     7801 ttaaggaggc tatcaaatta tgaaaaataa atttttttca aatgcgccaa tccatatcat
     7861 gcttatttta ggtggtattg caatggcatt accttttatt tggatgattt taacttcagg
     7921 gaaaacgttg gttgaatcta cccaaattcc acctacaatt ttccctgaga aatttcagct
     7981 aagtaactat caacaagtac ttgatttatt accattcatg gctttttata tcaatacatt
     8041 tttaatggtt gttggacgag tgattggctc ggttttattt tcagcaatgg ctgcatatgc
     8101 ttgtgcgcgt ctaagttttc cagggaaaaa tatccttttc agcttagtac ttattcaaat
     8161 gatgattccc ggttccattt ttattactcc acagtattta ttagcacaaa aattgggtat
     8221 tttaaataca attccagcat taatattacc aggggtagtt agtgcgtttg gtacgttttt
     8281 attaagacaa tttttcttag gtttacctga tgaattggag gaagcggcct atttagaagg
     8341 tgccaatatt tggacgatct ttttcaaaat tatgttgccg cttgctaaat caggattggt
     8401 tgcacttgga atattcacag cgttgtttgc atttaaagat ttaatgtggc cattgattgt
     8461 caatacttca attgataaga tgcctttagc tccgggatta gcaaacttac aaggacaata
     8521 tgccacgaac ttcccacaat taatggcagg ttcgttatta gcaatttggc caatagttgt
     8581 gatattcatc gttttccaaa gaaaattcat tgaaggaatt gctacaacag gtagtaaatt
     8641 ataaaactat taattagtga ttggagagta agtacatgac aaaaattaca tttattggtg
     8701 caggaagtac tatttttgca aaaaacgtat taggcgatgc gatgttaact ccaagtctac
     8761 aagatgcaca tattgcattg tacgatattg atgaaaaacg actaaaagaa tcaaatttaa
     8821 tgcttaaaaa tattaataaa aattcgaatg aaaatcgtgc tgatattgtt tcttattatg
     8881 atcgcaaaga agcgttaaga gaggctgatt ttgttatcaa tgctattcaa gtgggtggtt
     8941 ataaaccttc aacagtgatt gattttgata ttcctaaaaa atatgggttg cgacaaacta
     9001 ttggtgacac catggggatt ggtgggattt ttagaaccct acggacacta ccagtaatgt
     9061 tagactttgc taaagaaatg gaagaagttt gtccaaatgc ttggttattg aactatacaa
     9121 atccgatggc gatgttaacc atgggtgttt taaaagcgac gagtattaag actgttggtt
     9181 tatgtcatag cgttcaagta tgcgtaccag aattgtttga gcatttagga atagccgatc
     9241 aatatgactt aaatgacttt caatggaaaa ttgcaggcat taatcatatg gcattcttgt
     9301 tggaaattac aaaaaatggg gaagatttct atccaacaat tcgtcagtta gctgcagaaa
     9361 aaccaaatcc acatccagat tctgtacgtt ttgaattgtt gaaacgtttt ggttactata
     9421 ttacagaatc aagtgaacac aatgcagaat accatccata ctttattaaa tcaaaatacc
     9481 cagaattgat tgacgaatta gggattccgt tggatgaata cttacgtcgt tgtgaacaac
     9541 aaattaatga ttgggaaagc atgcgagagg acattgtaaa taatggggag ttaacacata
     9601 cgagaagccg tgaatatgct tcatatatca tggattctat tacgacagga actccgtcag
     9661 tgattgcagg aaatgtctta aataaaggct taatcacaaa cttaccgaca gatgtttgtg
     9721 tggaagtacc ttgtttagtc gataaaaatg gcgtgcaacc aacctatgtc ggtgaattgc
     9781 caacacaatt agctgcttta aatcggacga atatcaatgt tcaagaactg acagtagaag
     9841 ccgctttaac actagataaa tcaaaaattt atcaagcagc gtatttagat ccacatctat
     9901 catctgaatt gtctttaaca gatatcaaaa atttagtgga tgatttaatc gaagcacatg
     9961 gggactattt acctgaatat aattaaaaag aagctgaaag agtaaggaat tttcaattcc
    10021 ttactctttt cttagttaaa tttagtaaaa atattgacaa aacacttagt aaacagttac
    10081 attagtgtta acaaaaagaa aacgcattca aattgtataa ggaggagcat aaaatgaagt
    10141 ggtctaaaaa aattatttta gcttgtatag cactaggagc aacgatctct ttgactgctt
    10201 gtggaaaaaa tcaggaagat gcaagcggta aggtagaaat agagtttttt aatcaaaaaa
    10261 aagaaatgac tcaaacgatt caggagattg ctaaagattt tgaagcaaaa aaccctgata
    10321 ttcatgtaaa agttgtggat gtaccgaatg ctggtgaagt aattaaaaca cgtatgcttg
    10381 caggcgatgt accagatgtc attaatcttt atcctcaaag tatagaattg aaagaatggg
    10441 cgaaagctgg ttatttagag gacttaaccg aagaaccgta tttagagaat atcaaaaatg
    10501 gatatgcaca acgttttgct attgaggatc gtgtatatag cataccatta accgctaatg
    10561 tctatggttt ttactataac aaaactgctt ttgaggaaat ggggattcaa gcaccagaaa
    10621 cttgggcaga atttgaaaaa atagtagctg agattaaaga tcaaaacaaa gtaccgtttg
    10681 cgattgcagg agcagaaggc tggactttaa atggttatca ccaactagct atagcgactg
    10741 ctgctggtgg tgaagaggaa gcgaacaatg tttggcgttt ctcagaagtt aatggaatta
    10801 atgctgaaag caaagaaatg caaaaagatt ttaatcgatt agatttgtta agagaaccaa
    10861 aagctttgca aaacaactgg caaggtgccg gttataacga tacagtagtt acttttacaa
    10921 aaggtgatgc gttgattatg ccgaatggtt catgggcaat gccgatgatt cattcacaaa
    10981 atccagattt tgaagtagga acttttcctt ttccagcaga tgaagcaggt caaagtctaa
    11041 cgattggcgc aggagaccta gccttatcag tttctgcaac ttctcaacat aaagaagaag
    11101 ccaaacgttt tgtagaatat atgacaacac ctgaagcgat gcagaaatat tatgatgtag
    11161 atggtgcacc gtgtgctgtt gagggcgtta tcgagaatac tcaggattca ccattaagtg
    11221 gtttaactga acttgctttt actgatcgtc atttagtttg gttagccaaa gattggaata
    11281 gtgaaaatga tttttatacc ttaacaacaa attatttaca caatggcaat caaaaagcca
    11341 tgattaacgc attaaatgct ttctttaatc caatgaaagc tgacggaagg taggaaaata
    11401 aaatgaaaat aaaagaaatt ttaaatcgtt actgggcaat catttttgta gcaattccaa
    11461 ttattttgca gatgattttt ttctactttc caatggttca aggtgctttt tacagtttaa
    11521 ctaattggac tggtctgact tataattttg attttgtggg tttgaacaat tataagattc
    11581 ttttttctga tccgaagttt atcaaatcga ttggcttcac tttaattgtg acgctttgca
    11641 tgattgctgg acaaattggt tttggtattt tgattgcgcg ggcgttgaac gcaaatatta
    11701 aagggaaaac attctttaga gcatggttct tttttccagc ggtcttatcg ggcgtggttg
    11761 tttctttagt atttaaacaa atttttaact atggtttacc aaaaattggt agtatgttag
    11821 gcattgaatt tttacaaacc agtcttttag gaacaacatg gggagcgatt tttgcagtaa
    11881 tttttgtttt actttggcaa ggaattgcca tgccagtcat cattttctta gcgggttttc
    11941 aaagtattcc acaagaaatt attgaagctg ctagaatgga tggggcgaat gataagcaaa
    12001 ttttttggaa tattgaaatc acctacttat taccaagtat ttcaatggta tttattatgg
    12061 cattaaaagg tggcttaaca gcatttgatc aaatttttgc tttaactggt ggtggaccaa
    12121 gcgatgccac aacatctttg gggttacttg tttataatta tgcttttaaa aacaatcagt
    12181 ttggttatgc gaatgctatt gctttagtct tatttatctt aattgggatt gtttcactgg
    12241 cacaattaaa attatcgaaa aaatttgaaa tctaggaggt ttcccaatga aaacatcaaa
    12301 agaaaaaaat atttctaaat acttgctttt attattaggg ggcttcatga ttttagttcc
    12361 cttagtttca acgctattcg gttcatttaa aacaacaccc gatattatga ataatttctt
    12421 tcgttttcct aatccagcga ctttggaaaa tttccgaaga ttgttagcgg atggaattgg
    12481 tcattacttt tggaattcgg caatcatcac aatcattgca gtagttgctg tgacagcaat
    12541 tattcctatg gctgcttatt cactagcaag aaaaatgact cgtgggaaag catacggatt
    12601 gatgtattta tttttaattt taggaatttt tgttcctttc caagtaatta tgattccgat
    12661 taccgttatg atgagtaaaa ttggtttagc aaatatgtgg ggattaatct tactctatct
    12721 cacttatgcc gtaccacaga cactattttt atatgttggt tttattaaac taaatgtgcc
    12781 tatcagtctg gatgaggctg ctgaaattga tggggctagt cactttacaa cttatttcaa
    12841 aattgttttc ccaatgttaa aaccaatgca tgcgacgact attattctta atgctctatg
    12901 gttttggaat gatttcatgc taccattatt aattttaaat cgtgattcta atatgtggac
    12961 actaccattg tttcaatata attatacggg acaatatttt aacgactatg gtccaagttt
    13021 tgcttcctat acagtaggaa ttgtcacgat tacactagtt tatcttgctt tccaaagaag
    13081 tattatagct ggaatgagca atggagcagt gaaataacca ataaattatt ctcattataa
    13141 aggagttcat aaacttatga ttatatttga caatcacaca caaacatttc atttacaaaa
    13201 taaaaaaatt agctatctat taggaatcga agaaaataat tatttaacgc atctttattt
    13261 tggaaaaaag attacgcact attctggtgg gtatcgctac ccccgaacag atcgttcctt
    13321 ctcacccaac aatgcaaatg cgcaagatcg tttattatct ttagacacat taccattaga
    13381 atttccagga tatggacatg gtgattttag agaaccggcg cataatatta aattaggaaa
    13441 cggtagtcgt gtaaatgatt ttcgttatga tagctacgaa attcaaaaag gaaagcccag
    13501 cttagcggga ttgccagcct tatatacgga agatgatgat gaagctgaaa cgctgattat
    13561 tcaattgaaa gacgcgatta gtggattgac tttacagttg ttctacacaa tttatcaaaa
    13621 tcacagtgta attattcgtt ctacgcaatt agtaaataat agtgatgaaa cagtggagat
    13681 taaccgctta gctagtcaat caattgattt tccaaatcgt ccactcgagt taattcattt
    13741 aaatggtacc tgggcaagag aacgtcaaat gactagagaa aaaattcata cagggataaa
    13801 agtattagat agtaaacgcg gatcaagcag ccatcaacaa aatccgtttg ttgctttagt
    13861 agatgccaaa acagatgaat ttcaaggaga ggcatatgga tttaatttag tttatagcgg
    13921 taatcacgaa acggtgattc aaaaagatcc atttgaccaa acacgggtca ttatgggaat
    13981 taattcattt aattttggtt ggcaactaaa aactggcgac tcatttcaaa cgccagaagt
    14041 cgtgatggtt tatagtaatg aaggtctgaa tacgttatca cagacgtatc atgaattgta
    14101 taatcaccgt ttaattcgtg gaaactatcg tttaaaagaa cgtcccacgt taatcaacaa
    14161 ctgggaagca acgtattttg attttaacga agaaaaaatc atgaatattg tcgatgatgc
    14221 gaagaacttg ggcattgaaa tgtttgtttt agatgatggt tggtttggcg gtcgtgaaaa
    14281 tgacttcact tcattaggtg attgggttga aacagaaggc aaacttgctt gtggtttgga
    14341 aaatttagcc aagcaagtgc atgataacgg gatgaaattt gggatttggt ttgaaccgga
    14401 aatgatttct gaagatagtg aactatttcg agcacatcca gattgggcat tgggtattcc
    14461 aggtcgtgac cgctcaatta gtcgaaatca atatgttctt gatttttcaa gacaagatgt
    14521 tcgtgataat atctatcaac aaatgacggc gatttttgat cgtgtaccga ttgattatgt
    14581 gaagtgggat atgaatcgaa atatgacaga agtctattct gtcttgttgg actcaacgat
    14641 gcaaggtgaa gtggctcatc gttatatgtt agggttatat gaatttttag aaaagttaac
    14701 aacgaattat ccaactattt tatttgaaag ttgttcgggt ggaggcggtc gttttgatgc
    14761 aggtttcttg tattacatgc cacaaacttg gacgagcgac aataccgatg ccgtggctcg
    14821 tctgaaaatt caatatggaa ccagcttagt gtatccaatt tcaagtatgg gtgcgcatgt
    14881 ttcagcagtc ccaaatcatc aaacaggaag agaaacgagt ttaactattc gtggcaatgc
    14941 tgcgatggct ggtgtttttg gttatgagct agatctaacg caattaactt ctgaagaaaa
    15001 agcagaaatt aaagaacaag ttgcctttta taaaaagcat cgccaacttt tgcaatatgg
    15061 aactttttat cgcttggaaa gtccgtttga aggcaatgat actgcatgga tgtttgtctc
    15121 aaaagaccaa acagaagcca tggtctttta tttccgagta ttagcagaag ctgcgcatcc
    15181 attagtcaca ttgaaattag caggattagc agaagaagca acttatcaaa tggaagatgt
    15241 aacatttgct ggcgatgaat tgatgaatct cggtttttat attgactcag aactacatgg
    15301 tgattatgcg acacaacgct tttatttgaa acagatatag tagcttgaat ttacaatata
    15361 agtgcaacac caaaagagac tcattccaaa cgaagaaaat atgttttatt tacacgcatt
    15421 tactaaaaag cagcctgatt tgaattggga aaatgaagaa gtacgtgaag aaatctatgc
    15481 aatgattaac tattggttag ataaaggact aggcggattt agaatcgatg cgatattaaa
    15541 catcaaaaaa aaactagagt atggcatctt tgaatctgat ggagaagatg ggttagcctt
    15601 cattgggccc tggattctta atcagtcggg cattgaagtg tggttaaaag aaatgagaga
    15661 gcggacattt aaaccacata atagtatgac ggtagcagaa gccgatgttc ctaatgaacg
    15721 tttagatgaa tatattggtg aagatggcta ttattcgatg gtttttgatt ttagttatac
    15781 ggatattgat gtacctgaaa ctggtgaatg gtttaaagat agccaatgga catggacgga
    15841 tatgcgtacc aatattttta cgaatcaatt agttacgcaa gataaaggtt ggggcgcctt
    15901 atatttagaa aaccacgatc aaccacgttc aattaataaa tatattccag aagaatggat
    15961 caatgattat agtaaaaaaa tgttggcgac gctttttatg ttactaagag gtaccccttt
    16021 tatttatcaa ggacaagaac taggaatgac caacatagaa atggcatcat tagaagattt
    16081 tgatgatgta gctacgcatt ctcagtataa acgggcatta gaatatggat tggcaccaga
    16141 gcaagcattg aaagctgtca ataaacgaag tcgggataat tctcgcacac ctatgcaatg
    16201 gaatacgcaa aaaaatgcca gattctcaac ttctgataat gtatggttaa aagtaaatcc
    16261 taattatcca gaactcaatg cagaagctca gttaaccaat caacagtcgg tattcaatta
    16321 ttatcatcaa ttaattaaat taagaaaaaa tagtgtatac aaagacgtgc taatctacgg
    16381 acaatttcta ccagtgaaag ataaaaatga acatattttg ctttatgaac gccaattaga
    16441 agataaaatc gtattagtta tcttaaacgt tacagcaaca gaacaagaat attatgtaga
    16501 tgaacagtac caacaagtat taatcaataa ttatgaagat gttttattag atcaaagtcg
    16561 aaaattgcgt ttacgaccgt ttgaaagtat tgtattagct aattatggag gacaattatg
    16621 aaaattaaaa acgaagcaat gctcatcacc tatgcagata gtttaggaaa aaacatgggc
    16681 gaattaaaac aagtcttaga caaacattta caaggtgtta ttggtggtgt acatctgttg
    16741 cctttttttc catcgacagg tgatcgaggc tttgcgccga gtgattatac gacagttgat
    16801 ccagctcttg gaacatggga agatgtggaa gcattaggtg aagattatta tttgatgttt
    16861 gattttatga ttaatcacat ttctcgtgaa tcaaaatttt tccaagattt caaaaaaaac
    16921 catgagcagt caccatacaa agaaatgttt atccgtattc atgaattttt cccagaaaat
    16981 cgaccaacac aagaagatat tgatttaatc tacaagcgta aagacaaagc gccgtttcaa
    17041 acggtagaat ttgcggatgg tacgacagaa gaggtttgga atacctttgg ggaagaacaa
    17101 attgatttag atgtgaccaa agaagttgtg aaagaattta ttcgagaaac catcaaagat
    17161 atggcgaacc atggctgttc tttaattcga ttagatgcgt ttgcttatgc gattaaaaaa
    17221 ttagatacca atgatttctt cgtggaacca gatatttggg aacttttaga cgaagtgcgt
    17281 caagaagcag cgaaatatga cgtcgaatta ttaccagaaa tccatgagca ttattccatt
    17341 caaatgaaaa ttgccaatca tgattattat atctatgact tcgccttgcc aatggtcact
    17401 ctgtattcac tttatagtgg aaaatcagaa cgtttagcca attggttgaa aatgagtccg
    17461 atgaagcagt ttacaacatt ggatacgcat gatggaattg gtgttgtgga cgctcgtgat
    17521 ttattaacag atgaagaatt ggattacacc tctgaggaat tgtataaagt cggggcgaat
    17581 gtgaagaagg tctattcaag tgccaattac aataatttag atatttatca aattaacagc
    17641 acctattata gtgcattagg cgataatgac cgtagctatc tgttagcacg tgcgattcaa
    17701 gtatttgcgc cagggattcc acaaatttat tacgttgggt tattagcagg aaaaaatgat
    17761 attgaactgt tggaagcaac gaaagaaggg cgtaatatta atcgtcatta ctatgattta
    17821 gccgaaattg aagaagaagt gcaacggcca gtgattcaac aattatttgc acttttaaaa
    17881 tttagaaatg aatctgcggc gtttgatttg gatggaacga ttgaagtagc aacgccatca
    17941 gaatcagaaa tagtgattac acgtaaaaat aaaaacggta gtgatagtgc aacattaacg
    18001 gctaacttgg atacgaaaga atttatcatt gagtctaaag atataaaaat tttcactagt
    18061 gttgcattaa aattttaacc tattccatag aacaaaaaat cctttatact agcacttgga
    18121 tgacttatcc aagaccaata aaaaggactc atacatggat aagtataaaa caaatacagc
    18181 atttaataaa tggttttctg ccataaaatt aggaaaactt tctgtcaact cccaaaaatg
    18241 tatttttgat tacaattcgt atcgtaaaaa actgtctttt gaagctgtgc tcaagctctt
    18301 tctctacgca gttaatgaag aaaaagaaag tttgcgtgat ttgagtacag ctcttatcga
    18361 ccgatcgctc caactagaaa ccgatgtgtc atcgattagt catacccaat tgtctcgagc
    18421 atttaatgca cttgaaccta aagtgttaga agaaattttt caacaactgt tagaaaaagt
    18481 tcaacatcaa atcaaaccaa ccaagcgaaa tcatctgtat ctgattgatt ccagtacgtt
    18541 ttcgcttagt ttaaaacgtc ataagtgggc gactttccgt caaacaaaat caggtgtcaa
    18601 gttacactta aactattgtt atatggacga tactacgatg tatccaactg attttacctt
    18661 aacaaatgcc catgaacatg atctcaatca actaccagta ttggtgaatc aaccagaggc
    18721 aacttacgtg tttgatcgtg ggtattagga ctttgaaaag atggatcaga tgcattggga
    18781 tggctatttc ttcgtcactc ggattaaaaa aaacaccaaa gttcacgtga tagatactct
    18841 ggaaacatcg ccagaaacag aaattctacg agacgagctg gttcgtttag gatctaaaac
    18901 ctatctaacc gccaatttcc gactagtcac tgttcaagat aagaacggac gtgtgtttca
    18961 atttatcacc aatcgaatgg acgtctcttc aaaagaaatc tctgatatgt atcacgctcg
    19021 ttggcaaatc gaattgtttt tcaagcacat caaacaacac atgaccatta aaaccttctt
    19081 ttctcaaagt gaaaaaggcg tgcagaatca attgatttta acgatgattt ccgctctcct
    19141 cacgtttctc atcaaattgg agacgaaaac ggaaaaatca gtgttccaaa tcaaacgatt
    19201 ctttcgttat ttactgtttc agccctttga atgttgtcta gaaaaattaa ttcccacatg
    19261 agaaaaaaga aatttggctc gttacttttg gttttcagtt gaataacaga taaaaaattt
    19321 tctggaaaaa gtcatcctct gcgtcagcac agtttttttt gacttatttt cagaggaaat
    19381 agttttcaga atattctaaa atgttttatg caacactagt gatatttttt agaattagat
    19441 ttctaatgtg cattttttga gtattaagga gcaagtatac aaagatttat aaagctgatt
    19501 aaatttgaat aaaaatatgc aggaacgcta ctatttttag tagtgttctt ttttatattt
    19561 gaataaaaaa tgtggtggtt aaatggtggt tataacaaaa aatgaattaa tgaaattagg
    19621 ttatgggaag tatcagtctg agagaatcat acgacaggca aaagcaatta tggcttcaaa
    19681 aagctatacc tattacgaaa atagaagatt aggactagta cctgttgaat ctgtagaaga
    19741 agttttaggt atgaagatag aggtagagga ggattttcag aatcatggtt aattttaaaa
    19801 aagataaaaa tgggaaatat cattttactg ctagtttggg attcgacaaa cgaacaggga
    19861 aaagagtaca aaaaaacgta gaggggttaa aacagttaaa gaagaaaaaa agcgtatacc
    19921 gaacttgttt ctaattatgg aaaagaagca gaaacttctc aaagtactat gttgtttgag
    19981 gagttttttt ataaattatt cctaccttat tatcaaggga aagtagaaga aagaacatat
    20041 aaaaatcggt tatctacaat caaaaaaact tttcttattt ctttaaaatg gagttaagag
    20101 aaatacgtcc aattgacatt caaaagtggc aaaataattt attgagcaaa tataaaaatt
    20161 cttatgtaag aaacatgtat gggatttttc agatgtcatt agatcgagca gttgttttgg
    20221 gactacttga aaaaaatcct gcaaaagtta ttggaaatgt gaagaaaagg aaaactacag
    20281 ttgatttttg gacaaaagaa gaatttacaa aagttatttc aactttttat attgatgatt
    20341 tgtaccaaca ttttggtttt atttgtatgt ggttactttt tatgaccgtt atgcgtttgg
    20401 gagaagccac tgcattaact tggaaagata ttgattttga aaataaaaac ttgcgtgtga
    20461 ataaaacact ctattataag agagccaaaa cctatgagtt tgtagaaccc gaaacggctt
    20521 ctagtattcg tacaatcgaa ctagacgaca atacaattac ctttttaaaa gcttggaagg
    20581 agcgtcaagt ttcaatagga ggcatgtcgt ttgtactttc ttataatgga acacctactc
    20641 agaaatatac aatccgtcat attatgagaa gacatgcaac gatcgttggt gttcatataa
    20701 tccgcataca cggattaagg catagtcatg cttctttgct tatatcacaa taaaaagatg
    20761 cacttcgaat taaagaaagg ttaaaacatg ctgatgtaca aacgacttta ggaacttatg
    20821 gtcatttgta tctaaattca ttgagagaga taacaaatga tcccacagat tatatccaaa
    20881 ctaatttttc taaaatcaaa ccgacaccta tttacgataa taaaaaataa atgataaaca
    20941 aaagtaggat cacaccaaaa gaaacacact agaaaaacta taatacaggc ttttctagcg
    21001 tgttgtttct tatttccgcc tatttataca ttatactcat aaagcattta atgcatcgat
    21061 cgtagccgca ttgtctatat gggtatataa agttgttgta ttggtacttg aatgtcccaa
    21121 ttgttgggca gttaatacca atgaatttgt ctgttggtag agtctagtag ctaatgtatg
    21181 acgcagtttg tgagggctga cttgaattct aaagcctttt gaatattttg ctaccatccg
    21241 ttcgattgca ataccggata tacgcttggc ttctccttgg taaattgcta gaaataaagc
    21301 tttttcgttt tctgttactt tatatcgttc tttccgaata gctagataat tagctaaata
    21361 ttccattgcg aatgctgcta tattgacagc atctctttta tttccttttc tagttactac
    21421 cacattattc ttatctaaag ataggtcatc taaattaata tttataacct cggagagtct
    21481 caatccagat cctaaaatca gtgcattgat cgccaggtct ctttctacat ctcgttgata
    21541 atagatcaaa gctcgtttac tcaaagtatt tttatattca ttggcaatat aatctagata
    21601 attttgtgta tccttatcta gaaataattt tttctcaatg gcattggcac gtgcagctaa
    21661 cgtagtttta tctgtgatgg ttgctacttg atccatagga ttattgtcta agtatggttt
    21721 tcctagagga ttattggaac ttttcgaaag atatttaaat agaaccttta atgccgtgat
    21781 atttctattg atagtagaag tagatagacc taagttatta ttatctttga gcttagtttt
    21841 tgttgtctct ttttttacat tttcttttgt gcgacgtttc aaatgagatt tgtacaagtt
    21901 taggtcatag gcagttaact gtcttatcga ggtgatagaa gtatcaggaa cgatagactc
    21961 actcagaagc cattcataaa agagcttata attttttaga tattctaaag tggttcgagg
    22021 tgattgatct ttatcctcac gatcttgaac aaaatctttt atataatcgg gcatatgaag
    22081 tatgattgta tctatttttt gatatatttc tgtatttttc atgtcattca tcctttcaat
    22141 aagtagggga gaagagtatc tctataattg gactatacca attatagaat gttttgtcaa
    22201 ttggtatgct ctaaaattta ttaatttgtt tcaaaaaaag catataaaca ggtatacgtt
    22261 taataattta aaataaatgg tatacataaa tagggaagga aatagaaggg agcaatctaa
    22321 attttttgaa ttttcttaat taattatata gaaagaacaa tctttctata gagttagaca
    22381 tgagagggac tatgcttgat ttaaaatcaa caagcgtata tactgtatat acaaagaaaa
    22441 aggagggaga agactatggc aattaaagaa agaaaattta gggaagtggg gaactcagta
    22501 gtattaacta tttcaaagga gtttcttaaa gaaaatgggt taaatcccgg agatacggtt
    22561 tttttagatg aagaaaaact tcgagcggca attacaaaaa aacaagcctt atctgatgaa
    22621 attgatttag caatatctca agcattaact gaatatgata ctacttttag agagcttgtc
    22681 gataaatgag tattcattac ctagaagcca aagatattat aaaaatgaat gtaatccaaa
    22741 taaaaaaata ctctcctaat gaaccccttg gtgtaaagga tgcgaatgct ttagaaatgt
    22801 gtgtagggca acttcgagcc tcagcttttg gtgaagaggt ttatccttcg atttatgaaa
    22861 aagcagctat cctacttata caattgataa agaaacatcc ttttcacaat gcaaataaac
    22921 gtactgcttt cttagctact tttgtctttt tgaaaatcaa tggacagctt ttagctattg
    22981 atcaaaaaga tgctgtaaat ttagcggtat atattgctac gtatgataag gatttcgatc
    23041 aactaaaata tgaagtgatc aacactataa agacacatac gcagcccatt taaggagtta
    23101 ctgtatgtgt ctttataggg ttattagctg ctatagcgaa ataattgaag taaattacaa
    23161 atagctataa ttcaaatcca actatataga aaacgtaaat tttctata
//
LOCUS       DADVXU010000040        22733 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.042, whole genome shotgun sequence.
ACCESSION   DADVXU010000040 DADVXU010000000
VERSION     DADVXU010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 22733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.042"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(26..1957)
                     /locus_tag="KB953_001454"
     CDS             complement(26..1957)
                     /locus_tag="KB953_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBB6866436.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(1975..2889)
                     /gene="pfkB"
                     /locus_tag="KB953_001455"
     CDS             complement(1975..2889)
                     /gene="pfkB"
                     /locus_tag="KB953_001455"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBB6866437.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(2886..3638)
                     /locus_tag="KB953_001456"
     CDS             complement(2886..3638)
                     /locus_tag="KB953_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBB6866438.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(3797..4006)
                     /locus_tag="KB953_001457"
     CDS             complement(3797..4006)
                     /locus_tag="KB953_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866439.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(4017..5344)
                     /locus_tag="KB953_001458"
                     /pseudo
     CDS             complement(4017..5344)
                     /locus_tag="KB953_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5480..5635
                     /locus_tag="KB953_001459"
     CDS             5480..5635
                     /locus_tag="KB953_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866440.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(5709..6296)
                     /locus_tag="KB953_001460"
     CDS             complement(5709..6296)
                     /locus_tag="KB953_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBB6866441.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(6379..7629)
                     /gene="clpX"
                     /locus_tag="KB953_001461"
     CDS             complement(6379..7629)
                     /gene="clpX"
                     /locus_tag="KB953_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HBB6866442.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALSAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(7876..9165)
                     /locus_tag="KB953_001462"
     CDS             complement(7876..9165)
                     /locus_tag="KB953_001462"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBB6866443.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(9319..10251)
                     /locus_tag="KB953_001463"
     CDS             complement(9319..10251)
                     /locus_tag="KB953_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866444.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(10408..11067)
                     /locus_tag="KB953_001464"
     CDS             complement(10408..11067)
                     /locus_tag="KB953_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBB6866445.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(11070..12095)
                     /gene="sppA"
                     /locus_tag="KB953_001465"
     CDS             complement(11070..12095)
                     /gene="sppA"
                     /locus_tag="KB953_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HBB6866446.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(12108..12398)
                     /locus_tag="KB953_001466"
     CDS             complement(12108..12398)
                     /locus_tag="KB953_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866447.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            12807..14612
                     /gene="glmS"
                     /locus_tag="KB953_001467"
     CDS             12807..14612
                     /gene="glmS"
                     /locus_tag="KB953_001467"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBB6866448.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(14684..16039)
                     /locus_tag="KB953_001468"
     CDS             complement(14684..16039)
                     /locus_tag="KB953_001468"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBB6866449.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(16063..17232)
                     /locus_tag="KB953_001469"
     CDS             complement(16063..17232)
                     /locus_tag="KB953_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866450.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(17229..18116)
                     /locus_tag="KB953_001470"
     CDS             complement(17229..18116)
                     /locus_tag="KB953_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HBB6866451.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(18305..21997)
                     /gene="nifJ"
                     /locus_tag="KB953_001471"
     CDS             complement(18305..21997)
                     /gene="nifJ"
                     /locus_tag="KB953_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBB6866452.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            22196..22717
                     /locus_tag="KB953_001472"
     CDS             22196..22717
                     /locus_tag="KB953_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HBB6866453.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
BASE COUNT         6331 a         4939 c         4044 g         7419 t
ORIGIN      
        1 aaacccagcc tcttcttttt tatttctatg cttctgctac tgcaggagct tttctcaata
       61 aaccataaat cacaccagat acgactgctc cgatagcaat aaacaccaaa tacatgaccg
      121 ggtgactcag taagaataca acgaaaatcc cgccgtgagg agccagtaat ttgattccga
      181 aagctccgac taatccgcca gttaaagcag aaccaataat aaaacttgga atggcacgaa
      241 tcgggtcagc tgctgcaaat ggaatcgatc cttccgtgac aaacgatagt cccatcacga
      301 tgttcgtcaa accagcatct tgatctgttt tgctgaattt attcttaaac aaacgtgtag
      361 caacgaaaat tgccaatgga ggtaccattc ctccagccat aacagaagcc atcacgacac
      421 ttccgccagt tgcaacagtc gtagctaaag ttgctgtacc aaacacataa gcggctttgt
      481 tgattggacc gcctaaatct gccgccatca ttccagctaa taatgcacca agtaaagcag
      541 cattcgaacc actcatactt gtcaagaaac cgttcaatgc atcattgatt gctttcatag
      601 ggacattgac cagcaacatc aagaaaccag taatcagtaa gccaaacact ggataaaaga
      661 ggatcgtttt aattccatcc aatgattttg gtaagttctt gaataattta cgtaaaaata
      721 cgatgacata accagctaag aatccaccaa tcagtgcacc taagaatcct gcaccgcctg
      781 tattagctaa tgcaccagca gcaaatccaa cgatcaaacc agggcggtca ccgatacttg
      841 aagcaataaa tcctgctaat accggcagca tgaaaccaaa tgcagcttgt ccgatttggt
      901 tgaaccagct agcagcttga ttgtaattcc ctagatttga taattgatca tgtggcacgc
      961 caataaattg gtcgatcatg aaagaaagcg caatagcgat tcctccacca ataacgaaag
     1021 gtaacatgtg tgatacgccg ttcatcaaat ctttgtaaat tctttgtccg atagtaccat
     1081 ccgcatttcc ttcttctgaa ctgctttctt ttccatcacc atggaatact ggtgcagtac
     1141 cactaagggc tagattgatc aactcttctg gtttacggat accatcactg acaggacgat
     1201 tgatcagttc ttttccgtca aagcgattca tctctacttt tttgtctgct gcaacgatta
     1261 caccttctgc acgggcgatg tcttcagcag tcaatcggtt tttgattcct tctgaaccgt
     1321 tcgtttcgac tttgatctcc acacccattt ctttcgcttt tttcttcaaa gcatcttctg
     1381 ccatgtaagt gtgagcgatc cctgttggac aagctgtaac ggcaacaacg aattttcttt
     1441 ctggatgagc agaagcttct gcagcttgtg tttcttgttc tgctttttct tctgcttctt
     1501 tttgctcttc agctgtttgg aacaagtctt ggacttcttc tggtgtattt gcttctttta
     1561 attttcctac gaaatctgca tcaatcagca atcgagatag tgctgctaat gcttgcaaat
     1621 gtgtgtcgtt tgcgccttct ggagctgcga tcatgaaaaa cagataagtt ggctgtccgt
     1681 ctaaggcttc ataatctact cctttttggc tttttgcgaa taaaactgtt gcttctttaa
     1741 ctgcactgtt tttcgcgtga ggcatggcga ttccatcacc aagacctgta gaagtttgtg
     1801 cttcgcgggc tagaattcct tctttatatg tttcgatatc ggaaatccgg ccggcatcaa
     1861 acattttttg gaccatttca tcgattgcgc cttttttatc agtcgcttgc aaatccatga
     1921 tcatcgcttc tttgaccatt aaatccttga tattcatgat tatcctcctt gaaactaatc
     1981 aatttttgtg atttcaactt ctggcagcaa ctcatcaatc aattcgcgag ctgccaagtc
     2041 atcagaaaaa gctgttgcgc ttccgcaagc aactccccat ttaaaggctt cgattggatc
     2101 tttcgtacgg ctgtaagccc caataaagcc agcaatcatt gaatcccctg cacctactga
     2161 atttttcaat gggcgaacta atacattcgt tttataagtt ccttgttccg tgaagagcaa
     2221 agcaccgtct ccagccattg agatcagcac attctgtgcc ccttcagcta gtaatttttt
     2281 cccatacgga atgatttctt ctacagattg gaaggttgtg ttgtaaagct cagctaattc
     2341 gtgattgttt ggtttgatca acaacggttt cttaacaagt gcatttttta ggtcatcacc
     2401 tgttgtatca atcacgaaat ctgcaccttt ttctcgaatg atatcaatca gttcttgata
     2461 aaatcctttt cgcaggctag ctggcgcgct tcctgacaat acgacgatat ctccttgtcc
     2521 tactgttcct aatgcttgct tcaactcatc gatttcttca tctgaaatag atgggcctaa
     2581 gccattgatc tcagtttcag tgtctgattt tagtttgata ttgattcttg tatcttcttt
     2641 tacggttgtg aacaaagact ggatttgttc tttttcaagc cacttcgaaa taaattcgcc
     2701 agtgaaccct cctaaaaacc ctagtgctgt agattgagaa tcgatccgtt tcaaaatacg
     2761 ggaaacattg atcccttttc ctccaggtaa cttgaagtct tccttcattc ggttcaaatc
     2821 gcctagtttt aacccatcta cgcgtacgat ataatcaatt gatggattaa gtgttactgt
     2881 gtaaatcatt gtattgcctc cttaattgtt gtcttttcac ggaattcatt aacgaattct
     2941 aatggacaac gctcggttat gatcgtggct gcttccaaag gtgcaacaga ggtgaaggac
     3001 aactggttga atttggtatg atcaatcaat acaaatgtct cttcggaatt tttcatggct
     3061 aaacgtttaa cagctgcctc ttctggatcc ggagtcgtga atccttgttc aagatgggca
     3121 ccgttcattc ccataaatgc tttattaaaa tgaaaatttt ttagttgttc tatcgcatta
     3181 gatcctaaaa cagcattagt tgccagcttt atcgttccac ctaaaataat ggtagcaagc
     3241 tccaaatcaa ctaaatttgc cgcatgttga acggaatttg tcactactgt gatctgtttt
     3301 ccttgcagaa aaggaatcat ttctaacgta gtagagccag cgtctaaata aatgacatct
     3361 tcgtcattaa tggtttcagc tgccgatttg gcgatttgct gtttttcgtg aacgtttttg
     3421 agtgattttt cactcatatt ctgctcaaaa ttcaataatt gctctttctt agctccgcca
     3481 tgaactcttt ctagaaaccc actattctcc agctcttgta aatcacgtct tatcgttgat
     3541 tcagaagcat tcaaaaggtt tacaagatct tgtgatttca cgattttctg ctgttcaagc
     3601 aagtctagga ttttttgtct gcgttcctct gtaagcattt tcacgatcac ctcaatacaa
     3661 ataatagcat attttttttc tactagcaat ataaatcagt caaaaacgtt cataaaaatt
     3721 ataaatcagt caaaaaaaaa taaaaaacca gtcagaaaga ctggtttttt atttttttgt
     3781 ctatcatcaa gccgttttat cgttttttat tttcgcttct tgtttttttc atataaacag
     3841 aagcaggttt tctttcctta tgaagaagat aataataaag tcccatcccg ccaaacatga
     3901 ccaaaaaacc gatgactgat cgaataggaa aattaggaac aaaaaagaga cagacggata
     3961 ataatgatgc cggaaccatt attcgataat agagttgttt atccattttc ttcacctcat
     4021 attttttgta aagtaatcaa ttgttctaat tctcggtaat ctaaaaattg cacacgcaga
     4081 tgctgctcac ctttcttgac atcgaattgg atacttcgcc gctttttctt tattcgtttg
     4141 aaggtaacat cagagagtct atgtttgatt tcctttaacg gtaattctga cagggattct
     4201 gaaggaacat agattcttaa ggataaaaga tactcttgat atttttcatc tgttacctct
     4261 acttctagat attccctaat ataataacaa taagcttcga ttccattatc caaagcaaca
     4321 gtcggatcga taactttata gcacatcatg cgtgatcgat tctcgtcaac ccaaggaaaa
     4381 tacgcctgag catttagtgt tccgcgaaca cgcctttctt tataatctcc ttgttggata
     4441 tcataacttt ttgttacaaa aaatgaagca ttctttttag gaacaggttg catttgtgca
     4501 ttctgatact ggacagattg gatcgtatgg tcagtgtaaa tgtattcaac gaatggcggt
     4561 ttcaataaac cgacaccatt gtcccaagca agatgatcaa acccaagctg gtccctaaaa
     4621 taggattgaa acatttgatt gtttggcttt tgccagttaa aggggtctcg ataaagctgg
     4681 atacatatga cttcgttttc taattccttc actttttcta cttgttggat gtcgagaaaa
     4741 atcggtttag ggatgatttg atgataatag cgactataat acatttccca acacgaagtg
     4801 aaacataaac cttggtaaaa aagatcgtat cctggaaatt ggttgcaatc gactactact
     4861 tccccgtttt gttgtttcat tttaggtaaa tgttcgattt catccttttt ctcaaacaat
     4921 agccgttgag agatctgttt tgtattgtga tagataaact cgctataagc tcgcatatag
     4981 gcgaacaccc catgagcaat catttttttc tgaatgaagt ctcgaatcaa aaattgcttt
     5041 tctttgaaaa cagagataga caatacattt tttatcaata attgattatc ttccaagcga
     5101 aaactacatt cgttctcgtc atttcctaca actaaccaac tttcgtctct taccgatgct
     5161 agcattattt tttcttgaag ctttcgaaga tcatacggat ccgcttgttg tgtgaaagag
     5221 cggataaatg ttggctggat caacagacgt gtcaaaatag cccaatcgct tatttcctgt
     5281 ttttttcttc ttcatttaag tctgtgagct ggatagtcat caataattta tcttcgccaa
     5341 tcattttctc acctctttca aatcttttta taatttaatt ataaccaatg ataaagaaaa
     5401 actaaaactt tatgtcttag aaaccaattt ggacgattac atgatagact aagtaaaaag
     5461 aaacaaagag gtgtttacaa tggggttgaa atttgaacgt acagataatt tagataaact
     5521 tttcgaatca tttgctgtcg atccggaaaa aacagttgca ccaaaagaag agatcgatcg
     5581 atttctaaaa ccacagccac aaaatgaaaa gaaaaaagac aagaaacaaa agtaagttaa
     5641 gctcaaaaaa agacttacca gccaaacttt tatgaaagag tttgactggt aagtcttttt
     5701 tattcttttt atgattcaat tgcttgttcg attgctgccc acgcttcttc ttttccctgt
     5761 tttgtaacgg aagaaaagag aataaacgtg tcattgcgat caaaatccaa cgcttttttg
     5821 atttcgctct catgcttgtt ccatttacca cgagggatct tatctgcctt agtggcaaca
     5881 acaattacag ggatatcata atacttcaaa aattgataca tctggatatc ttcttgagaa
     5941 ggcttatgac gcaaatctac taaagaaacg accgcacgta gctggtctct agtcgttaaa
     6001 tatgtctcga tcatctttcc ccattttgct cgctcagttt tggatacttt tgcataccca
     6061 tatcccggaa catcgacaaa atgtaatgcg ttctcgatca aataaaaatt caatgtctgg
     6121 gtctttcctg gcttaccgga agtacgggct aaattctttc gatcaatcaa tgtgttgata
     6181 aaagaagatt tccctacatt tgatcgtcct gccagagcta tttctggcaa ttgtgtatct
     6241 ggatattgtt ttggtgccac tgcacttatg ataatttcag cttgatggac tttcatggtc
     6301 tctctcctta acaaggtgat gaataaagca agcggctgtc gcaaaagcca aaatgacttt
     6361 tacggcagcc tcttttcatc aacccgcttt tttctctgaa tctttataga ttactgttgg
     6421 ctcaccagac aattcagctg cttcttttgt gataatcact ttttcgatgt tttcatttga
     6481 agggatatca aacatcacat ccatcatgat ttcttcaatg atggaacgaa gtcctcgagc
     6541 accggtattg cgttcgattg cttttttcgc aatggcactc aacgcttcag gttcgaattc
     6601 aagttcggta ttatctagag aaagaagttt ttgatattgt ttgactagcg catttttcgg
     6661 ctcagtcaag atccgtacca gatcatctgt tgtcaatttc tctaaagctg ccataacagg
     6721 taatcgtccg ataaactcag ggatcaatcc gaattttagt aaatcttcag gaatgatatg
     6781 ctgcatgaca ctttcgtctt cagaaagttt ttggttttgt gtaccaaatc caatcgtctt
     6841 ttcgcccata cgattcttca caatcgtttc gatgccatca aatgcaccac ctacgatgaa
     6901 caatatgttc gtcgtatcaa tctgaataaa ttcttgatgt ggatgtttac gtccgccctg
     6961 aggtggcaca cttgcaacag ttccttctag aattttcagc aaggcttgtt ggacaccttc
     7021 gccggaaacg tcacgagtaa tagatacatt ttcgctttta cgcgcaattt tatcgatctc
     7081 atcaatatag atgatccctt tttctgcaag ttccacattg taatcagcag attgaagcaa
     7141 tttaagcaaa atgttttcta catcttcccc cacataacca gcttctgtca aacttgtcgc
     7201 atctgcgatt gcaaaaggta cattcaatgt ttttgctaat gtttgtgcta aaaatgtttt
     7261 accagaccct gtaggtccga tcaagcagat attgcttttt tgcagttcaa catcatctgt
     7321 gttttcttgt gcttgctgat tgacacgttt atagtgattg tatacggcaa cagctaacga
     7381 gcgttttgcg cgatcttgtc caatcacgta gttattcaat acttcaagaa tttctagtgg
     7441 tttagggact tcagtgaatt cgcgtacagc ttcttcataa aattcttcat caatgatttc
     7501 tttacatagg tcgatacatt cattacagat gtagacccct ggaccagcta cgatcttttt
     7561 cacttcttct tgggttttcc cgcaaaaaga acagcgtacg gtctcattgc tacttggatt
     7621 gtcatacatg gtcttcaccc cttaacttgc aaaaagtatg caagaaacta tcaaagactt
     7681 tacagtcttt tactatcata acatagttta gtaaaaagaa aaagtgttta acaaaggatc
     7741 aactgaccta tacctttgca ttttttactc ttaacaaaaa ataaggaact gtgacaaaat
     7801 cgtgtcacag tcccttattt cgaataaaca attttctaga agtagcttct tcagaaatga
     7861 tttattactt gatttctatt ctttcgcttc tgtcgctgta cctgtgatca attcgattgc
     7921 ttttttcatt gtcacatcat gttccagcat atcttctgtc aacacgcgtt tgatttgttc
     7981 gattggcatg ttgtatgttt ctgataattc ttggatttct gcattgattt cttcttctgt
     8041 tgcttgaaga ttttcagcag ctgcaattgc ttcgatcaca agatttgttc ttgtgcgcat
     8101 ttctgcttca ccttcgaatt gtttatgcaa gtcttcttct gtagaaccag ttaattgata
     8161 gtacatttca ggagagatac cttgacgttg catgttgttc aagaattcgt ccattgaacg
     8221 atgtacttca tcatgaacca tcacgtgtgg caattcaacg atttctgcgt tttcaacagc
     8281 catacggatc gctgcttcat cttttgcatc gtccgctgct ttttcttttg tttctactaa
     8341 ttctttgcga tatttttctt tcaattcgtc taatgttgat acttcgtcat caacatcttt
     8401 tgcgaattcg tcatctaatt caggcacttc ttttgctttt acttcatgga ttgtcacttt
     8461 gaagactgct tcttttccag caagatcttc tgcttgataa tcttctggga aagttacatt
     8521 tacatctaga ctttcacctg atttatgacc aactaattga tcttcaaaac ctggaatgaa
     8581 tgagcctgaa cctaattcaa gtgagtaatt ttcgcctttt ccgccatcaa acgcttcgcc
     8641 atctacaaag ccttcgaagt cgattactac tgtatcgcca ttttcagctg cagcatcttc
     8701 tttgataact aattcagctt gtgcttcttg ttcacgtttg atacgtgcat ccacgtcagc
     8761 atctgttact tcacggtctt gtttttcaac aactagatct ttatattgtc ctaattttac
     8821 ttctggtttc actgttactt cagcagtgat tacccagtct tgaccttttt ccatgctttc
     8881 aacatcgatt tttggttgag aaacaggatc gatgccagct tctttgacag cagcttcata
     8941 agcttctggt aatacagcat tcaatgcatc ttcatataaa gcttcttcac cgtacatacg
     9001 gttgaatact tgacgagaaa ctttcccttt acggaatcca ggtacgttca agcttttttt
     9061 cactttatta aacgcagttg ttaatccttt ttggatttcg tcttgcgcaa tcgtaaattt
     9121 taaaacacca tcattcgtgc cttttttttc aaatgttgca gtcatttaat tccctccgag
     9181 tttagaaatt cattttatac atcaaaaacg tttcatgcat acctttaaat agtacactaa
     9241 ccctttcaaa ttgtaaacat attgaatgga tagaatcctt gtttttcata acatttttat
     9301 ctttttcact gataaaatct aatggaagaa gatcatttgc tgcaaaattt tttctctttt
     9361 ttttaaaaaa tcttgttttt catcttctgt accttcatac atcgtttttc ctgtacacgc
     9421 ctctacagtc agttgaaacc agcgcttcgg gtcttcgatg tatgtatcat agacaggata
     9481 aacaattgca gattgcaacg tgaattcttc ttttaacacg gaactaagca gcgcatcttc
     9541 attcccgtaa cgatctgaaa atacacataa ttgctggtaa cttgcctgct gctgcggctt
     9601 tggcagatct tttggtatga ctttgactaa cttttcttca atcgtcaaat agaaaacagg
     9661 atggcattcg ttcaattgcc taagtgtctc cagtaatttt gcccttgcta aaatatggac
     9721 ctctggcgat acaataagtt tactcgctgt ttggataaac tcatgataag gaagctgttc
     9781 gatctcatca atagacgcaa gctgacgtgc aggttccaac gaaggaagtt cctccgattt
     9841 attgtggagt gccttgatct tctgcctctc catttgtccg gataattctt ctgcaaattg
     9901 gattttttca atgacccgct gctcgaaagc ttcataaaaa tctgtacttg ctatcaattt
     9961 tctagcatat aaaaaatctt gtgtactgat taacaagtca aaataaaatt cagcaaactc
    10021 ctcattttga agatactgtt tttcatgcaa gagtgcttgc ttcaatgctt cttttttctc
    10081 gtccaactcg taaagacaaa aaacaagcag gctattagcc tcaaagcttt ctttcaactc
    10141 gtaagctcgt tgaaataatt cttttgcctg ctgatattgc tggtttgcta gtgcttgttg
    10201 tgccactcgc attaaattct catatttttc tggaaaatca atcggcttca cgcagtgtcc
    10261 tcctcttgct catattcagc tcatgttcaa tagtagcaaa acaaaaaaag catgacaatt
    10321 aaaaataact gaagcaccct ctaaaagcta gattttagat ctgactttta gaaggtgctt
    10381 tcaactgtta ctgcttttta ttgctcatca gctgttgact tcctggttga acgctttgat
    10441 cagattgatc gtaacaacac ttgtattagt gaaaaaatcg cccagatgtt gctttttctg
    10501 attaaagact gctggaagat accctaacat cagcaaagga aattttagaa tgaaccggca
    10561 agcaccttct ctaactaaaa cggttgacca gctaagttct tcttcatcaa agctgatgac
    10621 tcgaatacca aagatcattt tcccaatcgt ttggccatga ttgaatttcg tcaataaaat
    10681 aaaatacgct agataaataa gtaaggctgt gaatccgtag agactcagat aagaatctga
    10741 aaaattaagc caacctagat ttttggcaat accaagtgtt aaacgcgtaa tagctccgat
    10801 acacaaaaga tcgaccaaga aggcaaacat tcggataaaa aaacctgcat agaaatactt
    10861 aggaaaatca tttgcttttg gcggttcatt tcttgtgtcc gtatactttt tccatttacg
    10921 ttgtttcttt ttgatttctt cgggcgtcaa aggctctctt tcaaaagagc tcagtacctt
    10981 ttgggaaaag ttggagtcag gtcctacagc aggctgctgt tgcttatctt tttcttgttc
    11041 cttattttca tttgaaagct ctgacatgct tactcacctc cgtaataata cattgctttt
    11101 ggtgattccg ctgtccctag attctcaaga acggataaga tctgactagt ctcagaaggc
    11161 ttcaatccct ggaattctgc tagtttactt cctaaccatg tactagcaaa cccagatggc
    11221 gtggaagaat attctactag ttccgcatct tcaagctgtt gttcctttcg tatagctttt
    11281 aaagcatctt caggaaatcc caattcatcg actaaaccta cttcttttgc ttggacacca
    11341 tcataaatcc ttccatcagc aatttttttg acttcttctt cagatttatt ccgtccctcg
    11401 ctgacgattg ctacaaaacg actatatgcg ctgtcgatat atgcttgaag caccgcttct
    11461 tcttcttttg tctctggtcg cgttcctgaa cccatatctt tcaacgctcc gcttttaact
    11521 gttgtatctt ccacacccag tttttctaga agtcctgaat aattcaatcc tgacatgatc
    11581 acaccaattg agccggtcac tgtttcttct gttgcaaaga ttttatctgc ttgagcagaa
    11641 atatagtagc caccgctagc tgccatgttt ttcatgctga catacattgg gatatcatgt
    11701 tctttacgaa tcgtatccaa tttcttagcg atttccgcac tttcgtagac accgccacca
    11761 ggagaattta cttctaaaaa gatacctttg actgttggat cttcttcaat cgccttcagt
    11821 tgttccataa aatttttatg attgtaacct tcagaagcaa ataaacctga agaccctccc
    11881 gcagcaatcg ttccatctac tgataatttt acgatttttt gcgtactgtc tccctcttcc
    11941 aacacatttg gagctagttc ttcatcccca tataaccaac tgttcaatcc ctcaatttga
    12001 tcgttttcct gaggcttagt caaactagct gaaactaacg aaaccacaaa taatcctgca
    12061 gcaatcagaa ctgctgccca acgtctttta ttcattcttt ctcctttcta atacaagttt
    12121 tttatgacga tcacagtttc tgactgctca gaagtgtcga accgctctgc tttttcttcc
    12181 cgcgcatacc cggaaaacga ctcttttgtt aattcatctt ctaccgtaaa ctcacgagct
    12241 tgagcaactt ccaaggcatc aggtgcgatt tcttggatgc tatcccagcc agtatctaaa
    12301 aatattttcg tatcatctga tacaccagct gatgcttcga atttggacaa ttgttttttt
    12361 aacattccca gtgtcatgat ctcaattttt gaatccattt ttactcctcc tctatccgat
    12421 tactttctat tgtaccgaac tcctgaaaaa gatgatagat aaaaacccat tattttttga
    12481 cataaaaagg acatttgaca aagaatttga tatataccaa tttatttttt attctttttt
    12541 tgttgacaag aacattcgca aaaggttatg attttaaatg tttccagata aaaaacaaaa
    12601 aggaggatag ttataccttt taggatagcg ccaggtctgt gtaaacagat gacgaggaga
    12661 gggtttatcg aaagattcgg cggatggccc tagggactgc actctacaga aaatccacaa
    12721 aaaatgtctg caaagataaa acaaaaggat tttcctgcag ctggaagcat tcatacgaat
    12781 tcattataga aagtaggatg ttttttatgt gcggaattgt tggaatgatt ggattaaaaa
    12841 atgtaacacc aggattgatt gacggtctag aaaaattaga ataccgtggt tatgattcag
    12901 ctggtatctt tgtctcagat ggcacaactg attatctggt caaagcacaa ggcagaattc
    12961 aaaatttgaa aaacaaaata aatattgata ctacaggaaa tataggtatt ggtcatacac
    13021 gatgggcaac tcacgggcaa ccttctgaag aaaacgccca tccacatact tcccaaagtg
    13081 gacgcttcgt gctcgttcac aacggcgtaa tcgaaaactt tgaagaactt aaaattgctt
    13141 atttagcaaa tgaccacttt attggtgaga ccgatactga gattatcgct catctaattg
    13201 aaactttcgc gaaggatact acaacgcaag aggcgttttt aaaagctttg cgggtcatca
    13261 aaggttctta cgctttcgct ttgattgatc ggacagcacc tgatgttatt tacgtcgcta
    13321 aaaataaaag tccactatta atcggactcg gcgatggatt caatgtgatt gccagcgatg
    13381 caatggctat gcttgctcat acgaaggaat tcgttgagat tgaagatgaa gagatggtta
    13441 ccgtcacatc agaaaaagtt attattcaaa attttgcagg tgacatcgta gaacgttctt
    13501 cattcgaagc acaagtagat gcttcagata tcgagaaagg tacctatcct ttttatatgc
    13561 tgaaagaaat cgacgaacag cctattatca tgcgtcgtat cgctcaaaaa tacgtaacag
    13621 aagacaatcg tgtctctctt gatcaaaagc tggtcgatac attgtctgat agcgatcgaa
    13681 tctatatcgt tgcttgcgga actagttacc atgcggggtt agcaggtaaa cagactttgg
    13741 aaaaattgac acagattcct gttgaggttc atttagccag tgaatttggc tataacacac
    13801 ctcttctttc aaaaaaacca ttctttattt tcttgagtca aagtggcgaa acagcagaca
    13861 gccgccaagt gctggtaaaa atcaaccgtc tcggctaccc ttcacttacg attacaaatg
    13921 ttgctggttc tactctttcg cgggaagcat cctttacact gctattgcat gctggacccg
    13981 aaatcgcagt agcatcaacg aaagcttata ctgcacaaat agctgtacta actttattag
    14041 ccaaagctat cggtgataaa aaagaatttg aaacttctct tgtattcgat atcactcatg
    14101 aattgagttt agttgctaat gcgatggaat cggtcatcgc ccaaaaagat tatttagaag
    14161 agttggcaga agtatatctc agcgatacaa gaaatgcctt ttacattgga cgtggtgctg
    14221 actacaacgt ttctcttgaa gctgctttga aattgaaaga aatcagttat atccaggcag
    14281 aaggttttgc cgctggcgaa ttgaaacatg gaacgattgc tttgatcgaa gaaggaacac
    14341 cagtgatcgg aatcatcagt gaagaagtga ctggtgcgca tactcgagga aacctgaaag
    14401 aagtcgaaag ccgtggcgct aaaacactag ttattgtctc agagggatta gaaaaggaaa
    14461 gtgaccagct ggtgctacca actgtccacc cttatctaac tactctaacc acagtcgtac
    14521 cgactcaatt acttgcatat tacgcaacgc tattacgggg attcgacgtt gacaaaccac
    14581 gtaatttagc gaaatcagtg acagtagaat aataaaagaa caaggaccag tgtcagacat
    14641 ctgacactgg tccttgttct tttatttttt atgaaaatat ctcttaatcc agtccgattt
    14701 cttctttgac tactgccgcg attttatcga catagtaatt aactttctca tcagtaggcg
    14761 cttctgccat cactcgaagt aatggttctg taccggaagg acgaactagg attcttcctt
    14821 ctgatcccat ttcagcttct gcctcttcaa tcactttttt gattgccgga acatccatcg
    14881 caccgttttt atcacttacg cggatattca ctaatttttg cggatagatt gttacctctg
    14941 ctgcaagctc tgataatttc ttacctgtct gtttcatgat attcagtaac tgaattcctg
    15001 aaagcatccc atctccagtt gtgttaaaat ctaagaaaat catgtgccca gactgctcgc
    15061 cgccaaagtt ataatcgttt ttgcgcattt cttctacaac gtaacgatca ccaacttgtg
    15121 taacgacatc tttcaaaccg attccttcca ccgctttgtg gaatcctaag ttactcatga
    15181 cagtcgtaac gatcgtatct tttttcaatc gatttttttc tgccaagtat ttagcacaga
    15241 taaacatgat tttatcaccg tcgacaatgt ttccaagttc atccaccgca ataatacgat
    15301 cgccatcgcc atcgaaagct agtccagcat ctgctccttt ttcaacgacc atctcagcta
    15361 aacgttctgg atgggtagag cctacgccgt cattgatatt taacccgtct ggatttgtgc
    15421 ccattgtata aaaatctgtt tcaagatcgg caaacaaacg attaacactt gttgaagttg
    15481 ctccgtttgc tgcatcgaca caaatggtca aaccggaaag gtcaccgttg atcgtttgta
    15541 caaggaattg agaatatttc aataagcctt ctggaaattc ttctacagtt cccaatcctt
    15601 cagctgatgg acgaggcaat tcatccactt cagcatctaa taatgcttca atttctagct
    15661 cttggtcatc tactagttta aaaccatctg caccgaaaaa tttgattcca ttatcttgtg
    15721 ctggattatg agaagcagaa atcatgaccc ctgcgcttgc tttttgcaaa cgtgtcagat
    15781 aagcaacacc tggtgtagag atcacaccta gctggaacac ttcgataccc accgataaaa
    15841 gtcctgaaat cagcgcttct tctaacattt gacctgagat tcttgtatcc cgccctacta
    15901 acacgcgtgg gcgttttcct tcttcttcat gctggcttag gacataacct ccgcaccgtc
    15961 ccagcttaaa tgcaagttct ggcgtaagtt ccttgttggc ttcaccgcgt acgccgtctg
    16021 ttccaaaata tttacccata gttgtaaaat tactccttta acttaatttt ctgaggaatt
    16081 tgttctgctc atttgtgttg attcgcttgt ttctgatgtt tcttctgtac ttgtggtcga
    16141 gctgcttgtc ccagattccg aactggaaga aatggttgtt gtcgatggtt ccgttcctga
    16201 atcagcagat gaaatacttc tactcgtact tgtattttct tgtgtccgac tgctcgtcga
    16261 agcagagcta ctgcttgacg tttgcaacac gggtgtaatc tccacactaa ctgattcggg
    16321 cttgatactt aacccctctt ctactggtat ttcaatttta cgagttgttc gatgattgat
    16381 acctgagaca ttgatcggta cagcaatact gtcaccgatc gatgcaagca aatcgctaga
    16441 gccgcttact tctgcttcca gttttgaaaa actgaaatca tagctttcta ccccggcagc
    16501 tggtgttcct tgctgtatcc cataaagtcg taccgacttg cttggcttga tcgaatcagc
    16561 tctcactgtt acttgcactg gatcagagac gatacttaat acttcccctg ctgcattcag
    16621 cgcctgtatt gggacctcac tattaatccc atcatctgtg accatcgaag cgtccacatc
    16681 agctactact cgtttgattt ctgccaatgt tttgtcacct gtagtgatag agactttttt
    16741 aggactaacg gaagtatcat caagttcata tccagatggt gtgaacgttg aaggaatcaa
    16801 cgtttccaca tcgaaatctt ttattacctt tttctcaacc gttactgtaa tagtcgatgg
    16861 ttcgatcttt gctgtgacag cactgctcaa attttgaatt tttagcggaa cttcatgtgt
    16921 tccttcgccc agcttagtga tatctgctgt gacgcggaac atccgtgtat cttcgtttgc
    16981 ctctgcattc aactgaactc ggtttgcact ggaaagtttg acagtcactg cattttcata
    17041 tccatggata tagtagcgct ttgaatcata agaaatcttg atcggcaccc ccgtaactgt
    17101 ttgattatac gcttcatttc cagaaagcgt atttggaacg gactggccat ttgcattgaa
    17161 gtacagcgca atactgaaca gcaatgcaat aaaactgtaa agaatgcttg tccgtttctc
    17221 ttttgagatc atttgcgatc ccctccctta gtcataccgt ctaggaaaga ttgaatcacg
    17281 tttttcttgt tttttgtttt ctcttcagca attagttctc tacgcaagat tgccaaatat
    17341 tcttgctggg ataagccagc catcagttca ttatctaacg tgatgctgac gtccccggtt
    17401 tcttcagaga caacgatggt aatcgcgtca ctgacttcgc ttataccgac agctgcccgg
    17461 tgtcgagtcc cgaattcttt agggatcaac atactttctg atagaggaag ataggcgctt
    17521 gctactgcga tctttccttc ttttacaata actgcaccgt catgcagcgg tgtattagga
    17581 ataaagatat tgatcagcag ctccccagtg atatctgcat ctaaagcaat ccctgtctca
    17641 atatactcat ctaatccagt atgacgttcg atagtgatca acgcaccgat ttttcgcttg
    17701 gacatatatt ggatggcttt gtcaaaagac aagatcatct tctcatcttc ctgctgttcg
    17761 ctttttgatt gtttgaaaag cgaactgcgt ccaagatgtt ccagtcctct ccggatttcc
    17821 ggctgaaaaa tcacgatagc agctatcacc ccgtacgtaa tgacttggtt catcagccaa
    17881 gacaaggtgt gcagaccaat gatttccgct acgattcgga taacaataaa aatagctaca
    17941 cctttcagaa gctgaatcgc ttttgtgcct cgcagcagtt ggatcagttt gtatacaaga
    18001 taccagacaa ccagaatatc aagtatgttc accaaaaaat tcagagagaa caagtcaccg
    18061 gaaaacattt gctgccaata gtccaaatta aaaagttccc cgatttgaaa tgacataaaa
    18121 agcctctctt ctcttttgag atttcctctc tagtatatca tatgttttaa ttttagaggc
    18181 tgatatgctc gtatttttct tgagaaaaac gcataatttt caagaaaatt gcaagtatgc
    18241 gttttatttt tctctgcaat aaatattttt aaacttcaga gaattattca ttatattttt
    18301 tctgttattt ttcctttttt tcttttgtag cttgttccat ttttttacgt cgttctgctc
    18361 tggcagctct tctagcagct cttctttctt ctgcttcaag gtccgtcgtt ttttcttttc
    18421 tagtcttaac gggtacatcg atagattcct ctggttcaag tcgtttcttg attttttcta
    18481 attctcccgt cagtgcagca tagttgtaaa aacgtgtttt agcgtcgtgg acagtctttt
    18541 caaataaagc ttttgcaaga tttggatttg cgttatctag cgaggaaaag cgaacttgtt
    18601 tcctcatgaa atcaggcata gcagagaaat caggtttttt gtagtccaaa gtcattgggt
    18661 tcttgttttt ttcgaccatt gtagggttat atcgatataa tgaccaataa ccagaagaaa
    18721 cagcttcttt tgcttcttta agcgattctt tcattccgcc aagcaagcca tgtgtgatac
    18781 aaggcgtata ggcaataatc agagaaggac caggatactt ttctgcttct tcaaatgcct
    18841 tgatcgtttg catttgattg gcacctgaag caatttgcgc cacataaaca tttccatatg
    18901 tcatcgccat cattcctaga tctttctttg aagcatactt tccactagcc gaaaatttag
    18961 cgattgccga agctggtgtt gcttttgagg tttgaccgcc agtattggaa tagacttcgt
    19021 tatccaaaac aaggatattc acgtctgctc cgctggcaag tacatgatcg atcccgccaa
    19081 aaccaatgtc gtaagcccat ccgtctccac caatcatcca ttggctgttt tttacgaata
    19141 gatcctttct cttgtatata tcctccagtt cagggaaaat tgatcgttcg ctttcgatcg
    19201 cagcttgcag ttttgctgct ctttgctgcg ttccttcact ctcaaacatg tgtactaacc
    19261 agtcttccat caattctcgt agttcttctg atacttttgg caatacttct ttgattgaac
    19321 ttgccaaagc ctcgcgacga gtctgtgccg ctagaagcat accataacca aattcagcat
    19381 tatcttccaa taaagaattt gaccatgcag gcccttgacc acattcattg gtcgtataag
    19441 gagaagcagg agaagcaccg ccccaaatag aagaacagcc tgtcgcattc gcgatcatca
    19501 ttcgatcacc gaacatttgt gtcaataatt ttacataagg tgtttcgcca cagcctgagc
    19561 aagcgcccga gaattcaaga agcggttgat tgaattgtgt agacaagaca gtattctttc
    19621 ctttgactgg atttgctttt tgtcttaatg tcattgcaaa agcccaattc acagcttgtt
    19681 ctttctgttc ttcataaggc ttcatcacta aagctttttc ttttgcggga caagcctcta
    19741 cacacagacc acaacctgta cagtcttcca ctgaaacttg gatacggtat tttacgccgt
    19801 ctgcgcctcg catttctctc acgataaatc cttctggcgc ttcttccatt tcctcttcat
    19861 ctgctaaaaa tggacggatg gcagcatgag gacaaacaaa ggcacattca ttacacatcg
    19921 tacagcgatc actaatccac tcaggtactt ctaaagcgac gccccgcttt tccatagaag
    19981 ccgttcctaa aggcatttcg ccagttgtca ttccatttgc tacaagtgta ccaacagaga
    20041 gttgatttcc ttcctgtgca ttcactggtt caagtatttc ttgtatataa ccagtgttcc
    20101 ttttattgat tttttcaggc atctccaacg ttctccaatg gtctgggatc gcgacttgat
    20161 gaagcagttc ggtcgtccga tcgattgctt gtatgttttt ttctacgata gccattgatt
    20221 tatgtccata gcttttcagg gcttcttctt ttagcagcgg aagcacctca tcaaaaggaa
    20281 tgatgtcagc taatttaaaa aaagccgttt ccattgccgt attgatccgg cgtcccaaac
    20341 cgacttcttg ggccaatctc atggcattga tcgtatagaa tctaatgtta ttttcagcca
    20401 agtaacgttt taattttaag ggcagatgcg tttctaattg ttcgtcgctc caaagagtat
    20461 tcaataaaaa aatgccacct ggcttcaatc ctttgaccaa gtcatagcta tgcaggtaag
    20521 ctggtgtgtg acaggcaaca aggtccgcat gctcgacaag atacgctgaa cgtatcggcg
    20581 tatctccaaa acgaagatga gagacagtca gaccgccaga ttttttcgaa tcataataaa
    20641 aatagccttg cgcatattta tcggtgtgat ctccaataat cttgatagct gatttatttg
    20701 ctccaactgt tccgtcagat ccaaatcccc aaaacttggc ctggaatgtt tgcggttctg
    20761 tcaaatccag actttccttt ggttccaaag attgataagt cacatcatcc acgatcccta
    20821 tggtaaaccg ttttttgcgt atgcttgggt cttttttcaa ttcatcaaaa actgctgaga
    20881 tttggtcggg agtcacatct ttagacccca atccgtagcg accaccaata acagccgggc
    20941 gaagttcaga atcatataac gcactttgaa cgtctaataa gagaggttct ccacctgccc
    21001 ctggttcttt acttcgatca agaacagcaa ttgtttttac tgtatgaggc aacttttgca
    21061 cgaatgtttc cactggaaaa ggacgataaa gatggatatt caaaaaacct gttttccgtc
    21121 cgtttgcatt caagtaatca atcgtttgtt caatcgtctg tgcaactgat cccatagaaa
    21181 caatgacttc ttccgcatcg tctgctccgt aataagtcac caagtcataa gaagttcctc
    21241 gtaatcggtt gatctctttc atatattttt gaacgatttc aggtaattta tcataatagg
    21301 ggttgatcgt ttcccgttgt tgaaaatgga tgtctggatt ctggttcgta ccactaacag
    21361 tcggatgatt gggattcatc cctcgtttac gaaatgaagc taatttctcc tgattgatca
    21421 gaggagctaa ttcttcatat tccaaaactt cgattttttg aatctcgtga ctcgtacgga
    21481 atccatcaaa gaaattcaaa aaagggactt ctccttcaat agaagcaaga tgggcaacag
    21541 gtgctaaatc catcacttct tgtacactgc tttcagctaa catagcaaat cctgtttgtc
    21601 ttgcagccat cacgtctccg tgatctccga aaatattcaa cgcatttgtc gtcactgccc
    21661 gactagcaac gtggaaaaca cttggcagta gttccccagc aatcttgtac atattcggta
    21721 tcatcaaaag cagcccttgt gaagcggtat atgtagttgt cagtgcacca gttttcaatg
    21781 atccatggac cacaccagct gctcctgctt cggattgcat ttctactact tttactggtt
    21841 gaccgaatac gttttctttt cctgctgctg accattgatc tactaattca gccatggtcg
    21901 aactgggtgt tattggatat attgcagcaa tctcggtaaa tgcataagaa atatatgcgg
    21961 ctgccgtatt gccatccatt gttttcattt ttcgcatgat tcatcatcct tgttcatatt
    22021 ctctcttatt atctgttcca ttataacgta aaacatgcca tttttttctt ttatttataa
    22081 aaatatttta catgtatcta tttgaattgg tcatctaatt tttagacaaa cagtctaaat
    22141 cgttgccttt ttttgtcaga catgtataaa ctataagcga aagaggtgtt ttaacatgga
    22201 tatatcagtg attgatgcaa cgaaaacaaa tacacaaaaa ggaatccttt atggttcatc
    22261 gaatgctcct aaaaaaatgg tcgaattcat caacttagct tgtccgtatt gccgacaatg
    22321 gtttgaagaa tcttatgagc tattagaaga agcagtccaa agcgggcaat tgcagcgagt
    22381 gatcaaattg ttcgataaag aaaaagaaag cttacagcga gggaacgtga tgcaccggta
    22441 tctcacaatc agcgatggcc aaaaagcgat caaagagatc aaacaaatat tcgatactca
    22501 agacgagtgg aaacatcttt ctctccaaga agtagcggat tttgcagtag ataaactaaa
    22561 gctgacagaa caaaaagacg aacagctgag ccaagctgtc atcaacgaag ctgagcaagc
    22621 acatattcga tttgttccaa ctgtcatctt gggtaaagaa atctttgatg aatctatttc
    22681 aatagaggaa ttaaaagaat tgatccaaac aaaataaaaa aataaggagc gtg
//
LOCUS       DADVXU010000041        22729 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.043, whole genome shotgun sequence.
ACCESSION   DADVXU010000041 DADVXU010000000
VERSION     DADVXU010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 22729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.043"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            165..1904
                     /gene="yfcC"
                     /locus_tag="KB953_001473"
     CDS             165..1904
                     /gene="yfcC"
                     /locus_tag="KB953_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBB6866454.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2061..3245
                     /gene="iadA"
                     /locus_tag="KB953_001474"
     CDS             2061..3245
                     /gene="iadA"
                     /locus_tag="KB953_001474"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBB6866455.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3356..3559
                     /locus_tag="KB953_001475"
     CDS             3356..3559
                     /locus_tag="KB953_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBB6866456.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3660..4322)
                     /locus_tag="KB953_001476"
     CDS             complement(3660..4322)
                     /locus_tag="KB953_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6866457.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4335..5837)
                     /locus_tag="KB953_001477"
     CDS             complement(4335..5837)
                     /locus_tag="KB953_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6866458.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6385..8064
                     /locus_tag="KB953_001478"
     CDS             6385..8064
                     /locus_tag="KB953_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBB6866459.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8191..8670
                     /locus_tag="KB953_001479"
     CDS             8191..8670
                     /locus_tag="KB953_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HBB6866460.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8889..9569
                     /locus_tag="KB953_001480"
     CDS             8889..9569
                     /locus_tag="KB953_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBB6866461.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9577..10908
                     /locus_tag="KB953_001481"
     CDS             9577..10908
                     /locus_tag="KB953_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBB6866462.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10923..11885
                     /locus_tag="KB953_001482"
     CDS             10923..11885
                     /locus_tag="KB953_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HBB6866463.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11996..12334
                     /locus_tag="KB953_001483"
     CDS             11996..12334
                     /locus_tag="KB953_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HBB6866464.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12413..13120
                     /locus_tag="KB953_001484"
     CDS             12413..13120
                     /locus_tag="KB953_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HBB6866465.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(13223..14704)
                     /locus_tag="KB953_001485"
     CDS             complement(13223..14704)
                     /locus_tag="KB953_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6866466.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLIKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            15013..15549
                     /locus_tag="KB953_001486"
     CDS             15013..15549
                     /locus_tag="KB953_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBB6866467.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            15627..16997
                     /locus_tag="KB953_001487"
     CDS             15627..16997
                     /locus_tag="KB953_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HBB6866468.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            17064..17399
                     /locus_tag="KB953_001488"
     CDS             17064..17399
                     /locus_tag="KB953_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866469.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(17480..18337)
                     /locus_tag="KB953_001489"
     CDS             complement(17480..18337)
                     /locus_tag="KB953_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6866470.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            18443..18958
                     /gene="tadA"
                     /locus_tag="KB953_001490"
     CDS             18443..18958
                     /gene="tadA"
                     /locus_tag="KB953_001490"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HBB6866471.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            18981..19065
                     /gene="ffs"
                     /locus_tag="KB953_001491"
     ncRNA           18981..19065
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="KB953_001491"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            19275..20660
                     /locus_tag="KB953_001492"
     CDS             19275..20660
                     /locus_tag="KB953_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBB6866472.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSCLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            20697..21374
                     /locus_tag="KB953_001493"
     CDS             20697..21374
                     /locus_tag="KB953_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HBB6866473.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            21382..22146
                     /locus_tag="KB953_001494"
     CDS             21382..22146
                     /locus_tag="KB953_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HBB6866474.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            22148..>22729
                     /locus_tag="KB953_001495"
     CDS             22148..>22729
                     /locus_tag="KB953_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HBB6866475.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVL"
BASE COUNT         7401 a         3819 c         4854 g         6655 t
ORIGIN      
        1 gaaagagaaa aaaataatca tacataggag aaaaaaaaca aaatagaaaa aagtgatttt
       61 ctaataaatt attcatgtgg aaatgtcatt tttactgtat aattcatttg attatccatt
      121 ttttattcat caaaattact gaaaaaagaa aggaagaaat aaatttgaag aaaacaaatt
      181 cttcaggaaa accgaaacgc gacttcacta aattatcgac accccatacg tatgtcatta
      241 tttttggtgt agtcatcttt gcatggatac tgacttttgt cgttccagca ggaaagttca
      301 gcacgcaaga tattgaatac aaagatgcaa atggcgaaac aagtactcgt actgttttac
      361 gtcaagattc cttccgttat gcttacgagc tggataagtc gtatgtgttt gatcaattag
      421 aagaactgca agatcatcca gcagaacgtg aaaaactgga tgttccggaa aaaggattgg
      481 aaaaagtcat tgctgacgga gaaaaaaatc ttacgcagga aaaactagat gaaatctcct
      541 tgacagatga tgtgctttac gatcagtacg gcgaaaatat ctatgatact tctaaaaaat
      601 tgcataaaac agccaaaata tggggaacag atgattttgg cggctttggt ttcttaaatt
      661 ttgtttttga aggactagta tctggagata agtatggatc tgctgtaggg atcgctgcat
      721 tgatccttgt tgttggaggt gcttttggta ttattatgcg taccggagcg atcgacgcag
      781 gtatctatgc gtttatcagt aaaactaagg gactggagcg tttagcttta ccgctattat
      841 tctttgcttt ttcattcggt ggcgctacat tcgggatggc agaagaggta attccattct
      901 caatggtgat ggttccattt gtcattgcgt taggatatga ctcgatcgta gcagtaaccg
      961 tgacttatgt cgcatctcaa gtcggaaatg cgacctcttg gatgagcccg tttagcgtag
     1021 cggtggctca agggattgct ggaatccccg tgttgtctgg tgcgacattc cgattgatta
     1081 tgtgggtagt tgttactgca ttagccgctg gttatatgat gatatatgca gaaaaaatcc
     1141 gtaaaaaacc agaaagttcg ttgacttaca aatcagatga ttatttccgt tctcatatca
     1201 aaaagacatc tgatgaaaat aaaaaattca tgcttgggca taaattgatc ttattagaga
     1261 tgttagttgt ccttgtctgg atcgtttggg gcgtgactca aaaaggctat tacattccag
     1321 aaatcgcttc tcaattcttt gtgatgggat tagttgctgg aatcattgct gttcttttca
     1381 aattagatgg aatgaaagtc aatgacatcg cttcttcttt ccaatctggt gcagctgatt
     1441 tagctggtac agcaatcgta gtaggtatgg ccaaagggat tcttcttgtc ttgggaggat
     1501 ctgatgcatc tgttccttca gctttgaata cgattttgca tgggatcggg acggcgttga
     1561 ccggtgttcc agctgtcatt ggtgcttggg cgatgtacat cttccaaagt ttgttcaact
     1621 tggttgttac atcaaattca ggacaagcag ctttgactat gccaatcatg gctccactgg
     1681 cagatttagt aggtgtatcc agacaagtag cagttcttgc ttatcagctt ggttcaggtt
     1741 ttatggacgc ttttacaccc gtatcagcca gcttgattgg tgtgctaggt gttgcacgga
     1801 tcgaatgggc aaaatgggca agattccaaa tcaagatgca aggattcctg tttgttttag
     1861 gaactatttt catcatgatt gccattgcaa taggtctgca gtagtactgg agacctgtaa
     1921 ataaaaaagt aatccaaaca gtagataagg actccaagaa ccgtcatttt acggtaaaat
     1981 caaaggagta aagatcctat cgtttggatt attttttaag atcctatcat cagttatact
     2041 taaagaaaga ggaaaaatgt atgaaaatca ttcgacaaat tgaagtgttt gcaccagatt
     2101 acttaggaaa aatggatgta ctgattgccg gtgggaagat tttggctgta gaagaacagc
     2161 tagaaggcgg ctacgaagga gtggaagtag aagaactttc aggtgaggga aaagtactaa
     2221 ccccgggttt cattgactgt catttccata ttttaggtgg aggaggagaa ggcggttatc
     2281 aaaatcgaac gccagaagtc acattgagcc aattgactac agctggtgta acgacagttg
     2341 tcggttgttt gggaacagac ggagaaggta gagatatgac tgcgttgatc agcaaagcga
     2401 aaggtctgga agcagaagga atctccactt atgtatacga aggttcgtac cgattgccgg
     2461 taaaaacagt aacagattct atcatcaaag actttttgac aattgataaa attatcggaa
     2521 tcggagaaat cgcagtatcc gatcaccgtt cttctcagcc aagttttgaa gagttcgctc
     2581 gtgcagtagc agatgctcgt gtaggaggca tgttatccgg aaaagctggg atcatcaatg
     2641 tccacttagg cggaggaaaa agaaagctag agctattgac acgaatcgta gaagaaacag
     2701 aaattccgat cacacaattt ttgcctactc atgcaaatcg cacaccagaa ttatttgaag
     2761 cttgcgtggc atttgccaaa cgtggtggca cgattgactt cactgctagc gaagatcctg
     2821 atttttggga gaagacagat ggtgaagttc gcttcagcaa agcactgaaa cgattgatcg
     2881 aggaagatgt ttctttagat aatttcacga tgacctcaga tggacaagga agcttgcctt
     2941 attttgatga aaacaatcat ttcctagggt taggagtagg aagtgccaag gcattgctag
     3001 tagggatcaa agaagctgtg caaaaagaaa gtatcccttt ggaaatcgcc ttacgagcta
     3061 taaccagtaa tccagcacgt atattgaaat tggataaaaa aggaaaaatc gaaattggtg
     3121 ctgatgcaga tctgtgtatc ttggataaag aaacgcttga tattgataca gttatcgcta
     3181 aaggagaaat aatggtccaa gaaaaagaag tcaaagtatg ggggactttc gaaaaaagtt
     3241 tttaatcaac agctctaact atctttttta gaaaacgagc gttctgtttg tctgtatctg
     3301 ttgttgatgt taccctgaag gtagatcaat aaacaaacta agaggaggaa gcacaatggc
     3361 agatttaaaa ggacgttcta aagatatgaa agataaagta gtcggtgaag gaaaagaagc
     3421 atacggcaaa gcaacagacg acaagagcaa agaaacagaa gggaaagcac aatcactagg
     3481 tgctgacatc aaagaaaaag ctcgaaattt aggcgacgaa gtcaaagaca agttttctcg
     3541 aaaagacgaa aaaaaataaa aaccacttat tactaattga atcaagcaaa taaaaaatcc
     3601 gaaccatatc tgcgagacag atatggttcg gattttttat ttgatcatta tcctgttttt
     3661 taaacttcga ttttttgaaa ttttttattt tttagttcaa agacttcatc acagcttttt
     3721 gctacttcaa tctcatgagt cacgatgata atacagcgat tttgttcatg ggcgatttct
     3781 tggaaaagtt tgacgatatc ttgtgaagtt gtttcatcta gatttccagt aggctcatcc
     3841 gctacgatta gttgatgttc acagcagata gcacgaacga tagcgactcg ctgctgctga
     3901 cctcccgaaa gtttggtcac ctgttttttt gctaatattt catcaattcc tacacgagcc
     3961 agtcgatcta aagcaacttg tcgataattc tcttgcttgg tcttagtgat ttccattgct
     4021 gctatgacgt tgtctagagc cgataaataa ggaagcaagt tgtaagcttg gaaaataatc
     4081 gagacatcat ttttacgata acttcttaac ccgattttgt tcagtgaacg atcttgatag
     4141 tatacctttc cttcttttgg tgcatctagt ccagaaataa gcgaaagaaa agtcgttttt
     4201 ccagaaccac ttgctcctaa aatagcgtac atttttccag cttcgaaatt cagttctaca
     4261 tctttataaa gcgcatcttg cggatcttga taccaaaatc ccaattgttc tgtccgtaac
     4321 atcaaattcc tcctttagtt taataggatt tttcttgggt ttaatcttaa tatccctata
     4381 gaagctaaaa agacagaaaa cagactgatt ccaaatgcta ctccggctaa tagtcccaat
     4441 tgtgcaggtt tgacattgat ttccaattcg ctgacttgat cagaaacagc aaatggatta
     4501 ttcgtggttc cttgttgacc atcgcgttga ccgccttgtt cttgtctgtt acctggcatt
     4561 tgtccaccgc caggagcttg ctggttttgt gtcgtggttt ctgtctgttg gtcaagtagc
     4621 tgctgtccca cggcattcgc gacgacgttt ccgctaagcg aagcaactcc taatgcaaga
     4681 atcatacaaa tagccacttc ggtaaacaat tggagaatga ttttacttct ggattctcct
     4741 aaagatagca agacgcctaa ctcatgccga cgctcccgaa tggtaatcat aatgatcaga
     4801 gtaagtatga taataccggc tacagccact agtaaaacaa catttttaga aaaacttgcc
     4861 acgttgttta aaggttccaa cattgactga tacatggaat catttgattg taagctaaat
     4921 gtatctgtgt cgatcagctt ctcagcttct ttgagaaact catccatttt atctggatca
     4981 gatagagtgt aaatagcaga atctatcgtg ttatcgtcag atgtcccatt tagttcattt
     5041 gcaaaagtgt aagaagtgta aagtgtattc gacggattca tgaagttgaa tttcataccc
     5101 atactgccgc ttgtttcgct ggattcataa attcctttga tcgtcatttc ataagtggta
     5161 tcttcatctt cagtactcgt gattgtgaaa gtatctccga ctgccaggtc atttgtttct
     5221 gctagagtag aatcaatgac tacattgttt gtgcctttgt cgctgtcatc tattccttca
     5281 ccatccgtga tttttgctgt tccatcagaa aaattactat tttgtgcagt tgctgacaca
     5341 ccactgattt gaaagtctgc ttgagtcatt tgcggattcc cgcccattcc tccagcaggt
     5401 tcaccgcctg catcagtagt ttctgaactg tcttcacttg aatcagaact agtgatggca
     5461 gttattccat cttttgccat agctgaggcg gaactttcaa aggaataact ctttacatta
     5521 tctaattcag ctattttttc cgcgtccgtt aaggaaactg gtgtaagaga aaaactgcct
     5581 ggatctggac gttcttcagt ggtagaaccg cttgaagaag tttgttcctg ttttttaaat
     5641 gaagcttctc gattggcact taaagtcact gttgctccaa cttctttttg tgcttgattc
     5701 gctgcttctt ctgcagcgct tcgaatcgtg aggccagcta gcacaaaaat aagaatggct
     5761 gtgaacacgg caaacaataa caatgaacgt ccccatttta cttttgtact tttccatgca
     5821 cgtttcataa aattcatatt ttgcatccct cctagtagat gcatttgata atagacaacc
     5881 tatcttaaag ctagcttaaa tcttatgctg tttgcacacc atcaacatga ggttctgtat
     5941 atataaaaag tcactatagt tattgcaaaa aatgtggtaa aatgaaaaaa gtatgtttca
     6001 aaccgacgaa gaagaaatga aagaggaata gcaaaagaaa tcctagatgc tacagaggga
     6061 tttttacgtg catagttgtt ctaacaattg ctgatactag cgatttagtt tatttgttgt
     6121 ataataatgg ggaacagctg aatacttata taagtattct ggctatagcg tttcgcaaaa
     6181 gatccatttt gttaatgaag tatatccaaa ataaaatgcc catccaactg tcagaacgcg
     6241 gatgtgctgg taattttcct gctgattctc cataagttga gaaatagtga ttttgcatat
     6301 gcttcattgg aatggctcat atatttagat aaagaagaaa atcgacatct ttaaacttgg
     6361 atgtcataag aagaggtgaa gtttgtgagt accattaaaa ttatcccatt aggcggcgtt
     6421 cgtgaaaatg gaaaaaacat gtatgtcgca gaagtgggcg aggatatttt tattttagat
     6481 tgtgggctaa aatatcctga aaatgagttg ttggggatcg acgtcgtaat ccctgatttt
     6541 acgtatttag aagaaaacat tgatcgtgta gctggtgtct ttttaacaca tggacatgca
     6601 gatgcgatcg gggcacttcc ttacttgttg tcaaaaattt ctgtaccagt attcgggaca
     6661 gagttgacga tcgagttagc aaaattgaat gtcagtcgta atgaagcagc caaaggattt
     6721 aaggatttcc atgtggtaga tgagcataca gaaatagact ttggtgaaac agtagtgagc
     6781 tttttccgta ccactcatac gataccagat tcagtgggga tcaatctgaa aacaaaagaa
     6841 ggtagtatcg tttatacggg agattttaaa tttgaccaaa cggctatccc aatgtatcaa
     6901 acagattatg cacgattggc ggaaatcggc aaagaaggag tcttagcctt actaagtgaa
     6961 tcggcaaatg cagaaaatcc tcagcctgta gcttctgaag cccaaatagc agatgaagta
     7021 tttgatacga tcaaatattg ggaagggcgt atcatcgtcg cttgtgtagc aagtaatttg
     7081 caacgagtgc aacaagtatt ggatgcagca gaaaaagcac aacgaaaagt tgtcttgact
     7141 ggtcaagatt tcgaacgaat tattcgcaca gcgatgcgtt tagaaaaact tcaattacca
     7201 agtgaagatc tgcttgttac gttgaaagac atgaaaaact atgatccaga agaactgatc
     7261 attttagaaa ctggccgaat gggtgaaccc atcaagtctc tgcaaaaaat ggcaaacggt
     7321 acacaccgta atgtcaagat tgaagaagga gatttggttt atatcactac tacgccaagc
     7381 atcgcgatgg aaaccatcgt ggcgaaaacg gaagatatca tttatcgagc tggtggaaca
     7441 gtgaagttga tctctgagaa tatgcgcgtt tctggccatg ccaatcctaa tgacttgcaa
     7501 ttaatgatca atttgatgaa accaacatat tttgtgccag ttcaaggaga gtatcgtgaa
     7561 ttagctgctc atgcagattt ggctcatgca gtagggatgc catacaaaaa tatctatatt
     7621 actggacgcg gagacgtgct ggaatataag aacaagcgta tgagtgttgc aggaactaca
     7681 agtgcagaaa atgtaatgat tgatggatta ggcataggtg atatcggaaa cattgtttta
     7741 cgtgatcgaa aaatcttgtc agaagacggg attttcgtag cggttgtcac tatcagtcgg
     7801 cgagaaaaga aaatcatttc aaaacctcaa attacttcac ggggattcgt ttacgtaaaa
     7861 gctagccgtg atctgatgaa agaaagcagc aatatgattg aaacaatcgt tgagaaacat
     7921 ttagaaagca atgattttga gtggagcaaa ttaaagcaag acatccgaga tcaattaggc
     7981 cgttacttat ttgaacaaac aaaacgccgc ccagtgatct tgccagtcat tatggaagct
     8041 actcaacgtc gaaataaaaa ataaacaaaa agagtaagat gaacctctct atagagtgct
     8101 cgtcttgctc tttttttgcg aaaaacaaat aatttttgaa tacagcatcc ctcgctttat
     8161 aataaacgat agaataaagg gagtgaatca atgatgagtc aaacaaaagt cgaacgttat
     8221 ttagaagaac aaaagatcag ctatcaacca cttgatttta gtcatgtggt agaagaatct
     8281 ttcacagaag aactacaaaa aagagggatt gatccagctt tgatatgtaa aactctcgta
     8341 gtgaaaggaa acaaaactgg ggtgatcatc gcattgattc ctttgaacga tcacttagat
     8401 tacaaaaaaa cgagacaagt gacgaaagat cgtaaagttg gttttcctgg aatggatttt
     8461 gtattaatgc atacaggata tccccacgga gcaaacaccc ctattggaat aaaactcgca
     8521 catcctgatt attgtttttt agcagacagt tctttaaaag aaaaagaaga agtaattgtg
     8581 tcaagcggtg aaatcgggag aagccttcgg ataaaagtag ccgatttaga aaagatcgtc
     8641 catcctattt acgctgattt aattagttaa gggacgagaa gagaagctaa gcagagcaga
     8701 gacctcaaac ttgagattat atctgcttgg tacttggtgc atcctgtatt atctgttaca
     8761 attgctttgt acatactgta ctatcaatcc aatgaaaata tatttagcag ctatattttt
     8821 tcattgatgt gcaagtgatg aaatcgtttt taataaaaaa gatacagaga aaacgcgagg
     8881 tgtaaaatat gaaaattacg tatcatggac attcctgttt aacaattgaa atggaaaatg
     8941 gccaacgcgt gatgattgac ccgtttatta caggaaatcc attgacagac ttaactgcag
     9001 aagaagtaaa gacagattgg ttgctgatta ctcatggtca tagtgaccac ataggagata
     9061 tgattcctat tgcaaaagaa aatcaagcca caattatcag tattgttgag attgctgatt
     9121 atgcgaacag cagaggagta gattcattcg gtatgaatat cggtggaaaa catgcgtttc
     9181 cattcggcgt ggtaaaattc gtacatgcac agcatagttc aagctatgaa gtagacggta
     9241 tcgttcagta tatgggcgag cctagcggaa ttattattca agcagaagga aaaacgattt
     9301 atcatgcagg ggatactgct tatttcagtg atctaggatt gcttgcagaa gaattcgata
     9361 ttgatgtcgc tttcttgccg attggcgata actatacgat ggggccagaa gatgcagcaa
     9421 aggctgcgca aacaattcgt gccaagaaag tagttccaat ccattacaat actttccctg
     9481 tcattgaaca agatcccgaa tcttttgtag agttactgga agatagtgaa ggaaaaataa
     9541 tgagcgtctc ggaaacgctg gaagtgtagg cagaaaatgg cgacaaaaca tgatttgatt
     9601 ttagaatata ttgaaagttt acctgttggc aatcgaattt ctgtaagaag catcgcaaaa
     9661 gaattgaatg tcagtgaagg aacagcgtac cgtgcaatca aagatgcaga aaatgtcgga
     9721 ctagtttcaa caattcaacg agtagggacc atccgtatcg aacgcaaatt aaaaaaacat
     9781 attgaacgtt tgacatttgg cgaggtcgtc aggatcattg aaggggacgt tttaggcggg
     9841 gcggcaggat tagacaaggt cttgaataaa ttcgtcatcg gcgcgatgac taaacatgcc
     9901 atggaacgct atattacgcc aggatcacta atgatcgtcg gcaaccgggc ggaagtccaa
     9961 aaattagcat tggaagatgg cgccgcagta ttgatcacag gaggattcaa tacgactgct
    10021 gagattgcaa agctagctga tgatcttgaa atgccgatct tacgcacctc ttatgataca
    10081 tttacagtcg caaccatgat caaccgagcg ttaagcgacc aattgatcaa aaaagatatc
    10141 atgcttgtca gtgatatcta tatgccagct gaaaagactc attatttgca gcagacagat
    10201 acgatttctg attatcagcg tttgtcagaa gaaacgcagc attcgcgttt tccagtcgtc
    10261 aaccgccatc atcgtttgat ggggattgtt acggcaaaag atgttttagg gaaatcgcca
    10321 aaccagatca tcgatcgggt gatgacgaaa gaaccaatca gtgtaaagaa aacaatgagt
    10381 attgcctctg ttagccatca aatgatctgg gatgggctag aagtcatgcc ggtcgtttcc
    10441 gatgacttga cattggaagg cttgattacc cggcaagatg taatgaaagc gatgcagctt
    10501 gtccagcgac aaccgcagat tgcagatacg atttcggatc agatatctgg aaatatccat
    10561 acgattgata ctgaccgtga aggaaatcgt ttggaaaacc ctaagttcaa attcgtagta
    10621 gcaccacaaa tggtcaatgg cgttggaacc atttcttttg gcgtacttag cgaaatcatt
    10681 tctgatgtcg cacaaaaaac aatggtgatg aaccaaaaac gaaatatctt gattgaacaa
    10741 gtgaacctac attatttgcg gctgatccaa ttggaaagcg aattagacat tcgtccacgt
    10801 gtattggaaa tcggcagacg ttccgctaaa ctggatatcg aagtgtttat tgaaaacacc
    10861 atcgtttcca aagcaatcgt tgtttgtcaa gtaatggaac gttcttagaa agcaggagtg
    10921 aaatgacagt acagcaagaa atagtagata caataaaaaa atacgataaa atcattatcc
    10981 acaggcattt gcgccctgat cctgatgcat taggatcaca agtaggatta gccgaactat
    11041 tacgggaaag tttcccagaa aaagaaatac gtcaagtagg tgaaacgatt gaagggcttc
    11101 gctttttagc agaaatgcaa gaagttgaag atgtcttcta ccaaggagcg ctagtcatcg
    11161 tgactgatac agctaattca cccagaatca gtgatcaaag atatcagttg gcagaaaaac
    11221 tgatcaaaat cgaccatcat cccaatgatg acccttatgg agatcttgtg tgggtaaata
    11281 caaaagcaag tagctgtagt gagatgattg cagaatttgc tttgatgttt cctgaatctt
    11341 taaagatgaa cgttaaggct gcacggttac tttatgcagg aatcgtcgga gatacagggc
    11401 gctttttgta tccctccacg acgtcccgta cattagagat tgcttcgata ttgagaaatt
    11461 atccatttga tgcaagtgct ttgaacagag agattgaaca aatgccgatg aaagtcgcta
    11521 aactttccgg ttatctttat caaaatatcc aggtggatga aaatggtgca ggaagaataa
    11581 tccttccgca agaaattctt gataaatatg gaatcgatga ttcagagaca gcttcgatcg
    11641 tttcacttcc tggagtgatt gacgaagtgc tggcttgggg gatttttgtt cagcagcctt
    11701 cagggtatta ccgcgtacgt ttacgttcta aggggccgat catcaatgaa ttggctaaac
    11761 gccatcatgg aggaggccat ccattagcaa gtggtgcgaa tgcaaaagat ttagcagaag
    11821 tagaggaaat ctataaagag atccaagata tatgccaatc gtataagagc agaaaaacag
    11881 aatgatactt cgttcgaaaa cgtgcctttt gtagtaaaaa tcacaaatgt atcttttcaa
    11941 gatcttcttt ttccgttaca atagtgctca ctaaagattt taaaggagaa agaagatgac
    12001 attaccaaat tgcccagaat gtggctcaga gtacacatat gaagatcgag gcttgttgat
    12061 ttgcccagaa tgcgggaacg aatggaacaa agaagaacaa gaaactgtca tagaagacgg
    12121 attagtggta ttagatgcaa atggaaaccg tctgttagag ggcgatagcg tgacagtgat
    12181 taaagacttg aaagtaaaag gtgcgtcagg tgccattaaa caaggaacaa aagtcaagaa
    12241 catccgttta gtagaaggcg atcataatat cgattgcaag gtagaaggat ttggaccgat
    12301 gaaactgaaa tcggaatttg tgaaaaagaa ttaaagaata ttcaaggtct tttcacattc
    12361 ttttcctact actaatgata aaataaagta aacgaaaagg agtgaaacga aaatgtatgg
    12421 ttatggaatg tatggttttt tagatccgac atttcttttg gtcattcttg gattagttat
    12481 ttccggaatt gcgtcagcgt acgtcaatag tactttccga aaatacgatc aagtgagaag
    12541 caaaaacaat gtgactggta cacaagcagc acaatatata ttacaaagtc aacagataaa
    12601 taatgtcggt gttcaacaaa ttgccggaga tctcacagat aactacaatt caggtaataa
    12661 gatgttgagt ttgtctcaag caactgcaca gtctacttca gttgctgcga taggtgtagc
    12721 agctcatgaa tgcggacatg cggtacaaga tgcttcgggt tatgtcccat tgaagattcg
    12781 tgcagcactt gttccagcag ctaactttgg ttcgatgatt tcgttcccct tgattatggt
    12841 aggtgtattg cttagctgga actctacgtt aatcaatatc gggattttcg ctttttcttt
    12901 agcattactt ttccaattgg tgacattacc agtcgaattt aacgcgtctc ggcgagcaat
    12961 ccaaattttg agtgaaggcg gactactgac ggaagaagaa gtaccgatgg caagacacgt
    13021 attatttgca gcagcactga cttatgttgc agcagcgctt tcgacattcc tgcagctgtt
    13081 gcgcttgatc ttattgttcg gcggcaaccg aagagattaa aataaagaag ttgtgaaatc
    13141 agcgctttga tctgagctaa taccgagagt tagctgattg aataccattt atttggaata
    13201 agaggatgtg acaagaattt tgtcacatcc tctttgtttt gttctcataa ccttttttca
    13261 tatacagttc atgaaccgct tttattaact tgccaaataa cactgtattg tgcgttgtat
    13321 ccagatagaa agtctccgtt tcatctgaaa ttttctcatg ctgatctgta atgatcaaat
    13381 cagcatgttc tgaatatgcg gtaattttaa cagcatcttt gcgaaataac tgctgcaaat
    13441 ggctttgaag atgataattt cctgtaaact gtttggttat ctgcaaatgg atgagtaact
    13501 gctctttcgt ttctgtttgt agtaagtgtt ggatcaattg actaaggtat tgactgattt
    13561 ggtttctttc cccatttttt gaaaaaattg tatcgatttt ttctcttaat ttcttttcct
    13621 gtataaaaga actagaatga ttggacgtgg cttctggaaa gcaaagtgta gaaaaactac
    13681 tgctggattc acccaacagt tcgaaaaaaa caactgccaa cacaagaaaa taaaaataaa
    13741 ttggtatttt attttctaaa tggttgagag gaaactgttg agttaagtaa gtcatcaatt
    13801 gaccgacttg tcgacaaata aggtttttgc ttgtggaaaa taaaaaacca atctcctgtt
    13861 tttgtcgaaa agagatttga tttggaaaaa aacaatagaa aaaataatta aagtaggctc
    13921 tctctgtttc tgtctcttca caagggcgac tcactgaaaa agaagtaaag tattctgaat
    13981 aatcatgtgt atgctgcaat aagtccatca attcagtgat ttcagaagaa gtttttggaa
    14041 aatatgcctg ttgtttgttt cgtaatatga ataaagttaa cagcaagtag atattttttt
    14101 cttgttttga aattttttct atttgctcat ctgtactgtt catttttagt gcatttaatt
    14161 caagtgagga aaatggccaa tcgttacctc caaatgtatc tgaaagaaaa aagtaaagat
    14221 agagacggat agataactca tttccttcaa gagtacagca attttttaca gataggatcc
    14281 gtaaatggaa ttttattaag gaatgattga gctgttttgt cagtttttcg agataggagg
    14341 gcgatatata aagttgttca agtaaatatt tttttggtac agcagtttgt ggataattga
    14401 ttagtaacat caaatagtta aaacgattgg attcttttag gtagcataat tttaacttag
    14461 aaaaaacagc taacttttta gtttgatcgg tttctttggt ttggatctct cctttatgat
    14521 tagaataaat aaattcatca atcaataaaa attgcttgag tgatacattt atttttcgga
    14581 tcagttcttt gattgtccgt tttggaatat gtaagtcatt ggataattct tgtaaagaac
    14641 aattattttt ttgaatgacc atttgtagaa caagtaactc tttttttctt gcttcaatta
    14701 gcataaatac ctccttttta ttctgttttt gtatatagtt agacaaaaac tgtgagtgga
    14761 aagtcatttc agtctgtgtt attctcacag agtgtcgcct tcttttttat ataacgttat
    14821 ttaatttttt tgttaccttg tttattctat tgtttatttc ctagggtatt ttgtcaagat
    14881 attcttgttt ttaaggcgga aaaattgatt tttaatggat ataactaaat atgttttgat
    14941 aattatcaat aatattttta gaattattta tgttatttat ttatgggtag atgaaaagag
    15001 acggaggtaa gaatgatggg gagagtaaca aattatagta aagaatattt gctgttcaaa
    15061 agtatggtat acgtggaaga atatggaatg aagtcaatta ctgcaagaga gttagcagag
    15121 ttctgcggtt gttctacata cccgatttat acacatttca aatcaatcag cgggttaaaa
    15181 gagaagatat tagaagaaat cactgtttat tttgaaaact acttagctga atgcagttcc
    15241 gatgatgttt taagcattgt ctacttattg aaagattttt tttctatcca tgaaagtatt
    15301 cgtgaaatca ttgcaaaatc tgatttagac atggattcgc tatttaagcg gccatttttc
    15361 tcatatgtca aaggtcacac gaagatcaaa gaagaaaagt cgcttgaatt ggtatggctt
    15421 aatgcattgg gaagtttgcg ctcagatgat tcttctcaag attttcttga aatgatgaac
    15481 gctgtttggg ataaaaacga ggtgacggat gaatacttgg aaaatatctt aaaggaacag
    15541 tcaccttagg cagcaacatg aaaaagtaga tcagccacct ttatttttcg taaaaaagat
    15601 tgtaaaatgt ctaacgaaac attggaatga aggaggaagc gacaacgtta ccacagcaat
    15661 ttatcaaaaa atatcgtctt cttttaggcg aagaagcatc cgattttttt tctgcgcttg
    15721 aacaaggaag cgtgaaaaaa ggatttcggt ggaatccact aaaaccggca ggattggaca
    15781 tggtccaaac ttaccatagc gaagaattac aacccgctcc ctacagcaat gagggatttc
    15841 ttggcactgt caatggaaag tctttcctcc atcaagctgg gtatgaatat agccaagaac
    15901 caagtgcaat gatcgttgga acagctgctg cagcaaaacc cggtgaaaaa gttttggatt
    15961 tgtgtgcggc tcccggtggt aaatcaaccc aattagctgc tcaaatgaag gggaaagggt
    16021 tattagtgac gaatgagatc tttccgaaac gtgcgaagat tttatcagaa aatatagaac
    16081 gatggggagt ttcgaacgca atagtgacaa atcatgcacc cgcagaactt gtccctcatt
    16141 tttcgggctt ttttgatcgg atcgtggttg atgctccttg ttcaggagaa ggaatgttcc
    16201 gaaaagatcc aaatgcaata aaagagtgga cagaagaatc tccgctatac tgtcaaaaac
    16261 gtcagcaaga gattttgtct tcagcaataa aaatgctaaa aaataaagga caactgattt
    16321 attcgacttg tacgtttgca ccagaagaaa atgaagagat cattagctgg cttgtagaaa
    16381 attatccagt aacgattgag gagattcctt tgactcaatc cgttagttca ggacgttctg
    16441 aatggggaag cgtagctggt ttggaaaaaa caatacgtat atggcctcat aaagatcagg
    16501 gtgaaggaca ttttgttgca aagctgactt ttcatggaca aaatcagatg cataaagaaa
    16561 agaagacacg aaaaaaaagt aaagtgcaaa tgacaaaaga gcaggaaaaa ctctggacgg
    16621 agttttcaaa tgattttcat tatgaagcta ctggaagact tttagtgttc aacgaccatc
    16681 tttgggaagt tcctgagtta gccccttctc tcgatggttt aaaagtcgta cgtacaggtt
    16741 tgcatttagg agatttcaaa aagaatcgat ttgaacctag ctatgcatta gcgcttgcta
    16801 caaaaaagat agagaatata ccttgcttgc cgatcactca aaaggaatgg caatcttaca
    16861 cagcaggaga aacatttcaa cgtgatggga atcaaggatg ggttttgcta gtattagata
    16921 agatacctgt cggatttggg aaacaagtaa aaggaacagt aaagaacttt ttccctaaag
    16981 gattgagatt tcactgagtg gattagttcg tttatttttt tgtctatgct atgatggaga
    17041 tagataaaaa ggagctgata cttatgaaaa catcaacaaa agtaacaatc ggattaagcg
    17101 tagcagccgc tgccagtgta gcgacagcag tcgttgtatc gggaaaagta atggaaaaga
    17161 tccatcatat gactactcgg acgaaagtaa aaaaattcgt ctatgacaaa tttgatggaa
    17221 atgaaaaatt actagaaatc gtagatggtc tttcagatag tgacttggat tctttgatgg
    17281 gaatgttagc aaaaatcaaa tcaggaaaaa agaaaatctc tgtttatggt gattcaatca
    17341 aagattcaac cgaagatgta aaagaccggc taatgcattt tgttgaaaaa atgatgtaag
    17401 agaaactagc ttttgaacac tattcattcg aaataagggg ttgtgacaaa attattgtca
    17461 caaccccttt aatctatctt taattatgtt catgaagcaa taaaagatag caaaacagta
    17521 aatcgttggc tttttttaga ttgaaaccag actgttcttg aaatttttcg attcgatatt
    17581 ttagagtatt acgatgaaga aacagtgttt ttgcagcaga gctgacattg cctaattcat
    17641 tccataattc ttgaatgatc acttccattt cattttgttt gatcagttct atgtaacttt
    17701 tgatcaactt actatctttt acagtatctt ttgtatagta atgtaaagcc acatcttgca
    17761 tagaaaaagt acggctgtga gtataaagat tgttttgctc tgttaaaaaa atttttcttt
    17821 cttcagcaaa caaagggacg aggtcggtgc caggtgaatg gaaagaacca acgaatacag
    17881 acgttgtcgt atcaaaatca gcatctaaag atagaaaaat accgttgatt tcagtagcat
    17941 caagataatc acggtttatt tgttcgacta agagcgcatc tgtgtctgta taaaaaaaga
    18001 aatcagcaga atagcgaaat aattccaaaa tagtatcttg ccattctttt tttaagaatt
    18061 ctccttttga ttcgacgtga agctggatca aacgaaagct tttttcttga acacaagaag
    18121 catctttaaa aagatagtga taccatgggt gttttttttg atcgttgatt acagggaata
    18181 acgctttcaa aaggctggtt tcttgttgag ataatgaatc ttgtttgatc cagataaaag
    18241 catcatcaat tgcaaaagaa aggatatccg cttctgatgc tggagagttt tttttctgtg
    18301 cttgtgggta aagagcaagt aatttatcca ttttcatctt gttcacctct tgtatttatt
    18361 ttaccataga aaaaaagtta gttacagcct ttttatcata tgctaaagta aaacaagaaa
    18421 aaacaaaggc agggaagggt aattggtaaa tacaaatctt gatgcagtag aaaaagaaaa
    18481 atggatgcgc cttgcattgg cagaagcaaa gaaagcagaa atgcttcatg aagttccgat
    18541 tggagcagtg gttgtattgg atggaaaagt gattggaaga ggatataacc ttagagaaac
    18601 cacgcaagat gctactacac atgcagaaat gcttgcaatc aaggaagcct gtgaaaaagt
    18661 gggaagctgg cgcttggaag atgctgcact ttttgttacc ttagagcctt gtcctatgtg
    18721 cagcggcgcg atgattttgt ctcgtgtagc agaagtttat ttcggcgcgt atgatccaaa
    18781 aggcggaaca gccggtacct tgatgaatct tttggaggac gagcggttta accaccaagc
    18841 atatgtagaa ggtggcattt tagaagacga atgtgggatg attttgactg actttttccg
    18901 aaagctccgc cagcgaaaaa agaaattaaa aaaagactgg tcaacgaatg aaaaataagg
    18961 tatactattc tttgccgaaa ggctaggaca atggtgggct tgtgaagcgt gtcaggtctg
    19021 gaaggaagca gcactaagca ggttccgcca tgtgtctgat tataatgaaa cgacaacaga
    19081 ctttcgggtc tgtttttttt atttttatag tactgtaaaa atgaatgttc ctgatttttt
    19141 agccagactg atttatcgaa tattgtactt atacaatttg cttcaaggca ggaaaaactc
    19201 tgaaatagag cttgtttcaa tggaagaaaa agctgcctat ggtatgataa agataaagag
    19261 aggcgagagg gtacatggaa aaaatagtga ttttacatac gaatgatctg cattctcatt
    19321 tagaaaattg gccaaggatc agaaggtttt tggatcaacg taaacgagaa aacgaaaaga
    19381 aagaaaaaac gacaacgatt gcagtagatc taggtgattt tgtggatcgt tggcatccat
    19441 tgtcagaagc aacgaatgga caggaaaata tcttgctgat gaatgaagtt ggttatgatg
    19501 cagcgacgat aggaaacaat gaaggcgtag gaaattccaa agaagagttg aatcatttat
    19561 acgatcatgc tgagtttgat atcatcctgg ataacttatt cgataaaaat acgctccagc
    19621 caccggcatg gacgaaacca tataaaatca ttacaacgaa acaccagacg aaaatcggac
    19681 taattgcatt tactgctcca tttccgctta cttataatcc gaatggctgg gacatacgga
    19741 atccttatga tattttgcca gaattagtcg aagatttacg ttcacaagtg gatgttttag
    19801 ttttgatgag tcatctcggg attcaggatg atttccaaat tgcaaaagaa ataccagcaa
    19861 ttgatgttat tttaggatcg cacacccatc atttattcag agaaggtcac gtaatcaatg
    19921 atgtccaaat agcagccgct ggaaagtacg ggcagtatgt cggtgaagtc catttaacca
    19981 tcgatgaaga gaaaaaaatc cttaagcatt cagccaaagc catccctacc gaaacaatga
    20041 cagcttttac agaagatgaa caagaaataa atagttgtct agaacgtggg catgaattat
    20101 taaaagaaaa agaagtagca gatttgccat tttctctgag tctagatatc ttcaatgagc
    20161 attcgtttat ccaagtagcc tttgaggcgg tgaaagaacg aggaaagacg gaagcagcaa
    20221 tattaaacag tggtctgttt ttaactgaaa ttccaaaagg gcgggtcaat caggatcagc
    20281 ttcacactgc gttgccgcat ccgatgcact tgttaaatgt cacattgggt ggaaatgatc
    20341 tgatccgctt agttttagaa atagaaaaaa atcgtaactt tttacgtaac tatcctatga
    20401 agggaatggg atttcgcggt aagatttttg ggcagatggt ctataatgga atcacatatg
    20461 attctattaa ccatcaagtc ttttggaaga acaagccaat cgatctgaat aaaaattata
    20521 cttttactac cgtagatcat ttgatgtttg ttccattttt tccaacgatt gagattgctg
    20581 gaaaaaatga atttttgttc cctgaattta tccgaagtgt ggtaggagat tacttgaaag
    20641 ctcactaccc aatcaaataa aaacaaggta taataaaaaa gataggtggt gaacgcatgt
    20701 ctgcaaaaca aaatgaacag cagaacgttg agcataaaac agaaattgct aagaaaaaat
    20761 catcagctga acaggaaaca acagtcaccg aagaactgac agctgctttg gaagaaatcg
    20821 ttgaggagcc taaagcagaa aaagtcaaag gcgagctagt gacactgtta gatgacagta
    20881 attttttgat aggtgaaaga cattatcggc ttgtgtccga ttatcgtgaa ggatttaatg
    20941 cagaaaagct tggagaacgt tacagtgatg tactagcccg atatgattat atcgtaggag
    21001 attggggtta tgaacaacta cgcctaaaag gatttttcca agcggataat cgacgggcgc
    21061 atccagatca gcgaatcgat tccttggaag actatcttta cgaatattgc aattttggtt
    21121 gtgcgtattt tgtcatcgag agaatcggcg gtaaaaaaga aaagacaact cagcgccgaa
    21181 aaaagaaaaa aaaccataat cgtgcgtata tcgatgagaa aaaaggacca gtcgcatcta
    21241 atcgcaaaaa accggttatt aaaaatcgaa aaacagagtc agcaaaaacc tctgaaaaag
    21301 taaaaaaaca agaacaacct aaacaagtag aaaaaacaaa aacagcattt acgatcagaa
    21361 aaagggaaga gtagtggatg aatggactat aaaggatatt taattgattt agacggaacg
    21421 atttatcgtg ggacacaacc cataccggcc ggtaaacgat ttgtggaaga attgcaaaaa
    21481 agaaaattac ctttcttgtt tgttaccaat aacacgacta agtcaccaga aacagtagct
    21541 aatcgtctag cagatgagtt tgacattcat gtagctccag agacagtcta cacagctact
    21601 ttggcaacaa tcgattttat gaaagcagat ggaaaaggga aaaaagtcta tgttatcgga
    21661 gaagcaggat tgattgactt gattttagct gccggattta catgggaaga agaggctcct
    21721 gactatgtag tggtaggctt agataattat ctgacctatg aaaaagtagt taaagcaaca
    21781 cttgctattc aaaaaggtgc tactttcatt ggaacgaacc cagataagaa tatacctact
    21841 gaacgcggcc ttcttcctgg agcagggtca gtcatctctt ttgtagagac agcaacccaa
    21901 acgccgccga tctacatcgg aaaaccagaa gccatcatta tggataaagc cgttgaagta
    21961 ttagggctgc aaaaagaaga agtaatcatg gttggagata actatgaaac agatatccaa
    22021 gctgggatac gcaataatat agatactttg ctcgttttat caggttttac aaaagaagaa
    22081 gatgtacctg ggttgcctat cccagctacc tatgtgaaac aatcactgga tgaatggagc
    22141 ttctaatatg aaaaattcga gatggcagtg gatggaatat gctggattgt tcagcctatt
    22201 tctgacactg atcagtctgg cagttggtgt gacgatcaac tttcgaccac tatacgtatt
    22261 tgatatcgga catttgcaaa ttttggatta tacttcttta gatcaagaga cgttactgaa
    22321 aaattttgat cacctaatga attatctcaa taatcccttc aaaaccatcc tcagcttgcc
    22381 agatttccca gtttctgcta gcggagccca tcacttttat gaagtgaaaa ttttgttttt
    22441 agtggattat gctgtgtttt tcatcacttt gatcccgagt attttgttta tcaaatattt
    22501 gcaaaagaat gatcggcttt ggcggttgat tcgtccgttt caaatcggca tgttactgcc
    22561 agttgttttc ggttttttca tgatgatagg atttgatcgc ttttttatcc tttttcatga
    22621 aacgtttttc aacaacgatg actggttatt cgatcccgtt actgatccaa tcatcaatgt
    22681 acttccagaa gaatttttca tgcatagctt tatcttattt tttgtatta
//
LOCUS       DADVXU010000042        22729 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.044, whole genome shotgun sequence.
ACCESSION   DADVXU010000042 DADVXU010000000
VERSION     DADVXU010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 22729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.044"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            133..3660
                     /locus_tag="KB953_001496"
     CDS             133..3660
                     /locus_tag="KB953_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HBB6866476.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="KB953_001497"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="KB953_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HBB6866477.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="KB953_001498"
     CDS             7448..7789
                     /locus_tag="KB953_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6866478.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="KB953_001499"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="KB953_001499"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBB6866479.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="KB953_001500"
     CDS             9146..11566
                     /locus_tag="KB953_001500"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBB6866480.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="KB953_001501"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="KB953_001501"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBB6866481.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="KB953_001502"
     CDS             13000..13737
                     /locus_tag="KB953_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBB6866482.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="KB953_001503"
     CDS             13751..14566
                     /locus_tag="KB953_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBB6866483.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="KB953_001504"
     CDS             14579..15226
                     /locus_tag="KB953_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBB6866484.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="KB953_001505"
     CDS             15242..15898
                     /locus_tag="KB953_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBB6866485.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="KB953_001506"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="KB953_001506"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBB6866486.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="KB953_001507"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="KB953_001507"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBB6866487.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="KB953_001508"
     CDS             18240..18758
                     /locus_tag="KB953_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBB6866488.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="KB953_001509"
     CDS             18865..19365
                     /locus_tag="KB953_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBB6866489.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19645..20565)
                     /locus_tag="KB953_001510"
     CDS             complement(19645..20565)
                     /locus_tag="KB953_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HBB6866490.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20730..21743
                     /locus_tag="KB953_001511"
     CDS             20730..21743
                     /locus_tag="KB953_001511"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HBB6866491.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="KB953_001512"
     CDS             complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="KB953_001512"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HBB6866492.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
BASE COUNT         7573 a         3957 c         4832 g         6367 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga
    19621 gattcgttgc atcgtttgtc caaattattt tccgacagca attggactgt tcggaatcgt
    19681 gaaaccagaa gctgtcaatt ctttgacata ggagctcaag aactcttctt ttaaggcata
    19741 ttgttttccg ttcaatgcgt agcagaccgt acgaatcgca aaattgctgt ttccaagatc
    19801 cactaatcca aagatagaag gttcagtttg gagctcttct tggtattttt cagctaatgt
    19861 acgatttact cgatcaatga tctcatagat tttgtcatat ccttcttcag gaacaatccg
    19921 aatatctaaa agtacttgca tatttgcacg agagagattg ctgatcgtgg tgatgtttcg
    19981 attcgggatg aaatggacag taccatccgt tgctttcagc tgaagggtac ggataccaac
    20041 agatacgacg gttccttcaa tcgttaagtt tgctagcttg atgtagtccc caacatccat
    20101 ctgttgttcc atgataataa aaaatccggt aataacatca ctcatgaacc cttgggcacc
    20161 aagaccgatt gccacacctg caattccggc accagccaaa agtgatccga tcggcacacc
    20221 gatagcagat aaaattgcat agataaaaaa gaaaccaagg gtataatgaa aaatagtatt
    20281 taaaagacta tgtaaagtct ttaagcggct ctcgtttaga atggcttttt tagaatactg
    20341 attaaaagac ttatcaatca gatattttcc cacacggtga agaatccaaa aaaggacgat
    20401 caaaaaaatc agatataaac ttttttggat aaaggcagcc aaaatggcgt cccaatcaat
    20461 cccgtcccaa aaccgctgaa acgcatttac tttgctgaca gcttgatctg tcaatgtctc
    20521 gccagaagag tctgtcgtac cggcagttgc taataatgaa aacataaaat cctcttttct
    20581 aaaatttgat tctttctgta ttgtaccaag aagttttcaa aaaaggaaat agcaaaaata
    20641 actataatga aggaaaaaag ctgaaaataa aatgacaaat aatatggata atgataaaat
    20701 gtggagaaaa taacgaaggt ggtaagtata tgcaaatcga ttggaacaat ctaggttttt
    20761 cttatatcaa aactccttgg cgttttattg ccaaatggaa agacggacaa tgggaagaag
    20821 gagaattgac agaagataat tacctgacga ttcatgaagg ctctccagcg cttcattatg
    20881 gtcagcaatg ttttgaaggt ctaaaagctt atcgtcgaaa agacggcaag gtcaatctct
    20941 ttcgacctga acaaaatagt cgacggatga atcaaagtgc tagacgattg ctgatgccaa
    21001 tggtccctga aaagatgttc gttgatgctg tcaagcaagt ggtaaaagca aatgaagatt
    21061 ttgtgccgcc ctatggaaca ggcgctacat tatacttgcg ccctttactg attggggtcg
    21121 gtgaaaacat tggtgtccat ccagctccag aatatttatt tgtcgtcttc tgtacgccag
    21181 taggagcgta tttcaaaggt ggactaaaac caacgaattt tattgtatct gattacgatc
    21241 gtgctgctcc taaggggact ggtgcagcaa aagtcggcgg aaattacgca gcgagtttac
    21301 ttccaggaac agaagcaaaa gaactggatt actcagattg tgtttatctt gatccagcca
    21361 cacatacgaa aatcgaagaa gtaggtgcag caaatttctt tggaattaca aaagacggaa
    21421 aattcatcac tccgcaatcg ccctctatct taccaagtat cacgaaatat tccttgttgc
    21481 agttagctga agaacgacta ggactgcaag caattgaagg agatattttc attgatgatc
    21541 tagatcaatt tgcagaagct ggtgcatgtg gtacagcggc tgttatttct ccaatcggtg
    21601 ggatttatca caataacaat cttcatgtat tttatagtga aacggaagta ggaccagtaa
    21661 caaaacggtt gtatgatgaa ttgacaggta ttcaatttgg agacatagaa gcaccagaag
    21721 gctggattga agtcgtggaa taatttcaaa gtaatgagtt gaaaaaaacg taaaaagaaa
    21781 aaatcaggaa gagactttaa aaagtttttt cctgattttt atatgtttta ctttaaagaa
    21841 tgcaacaaaa gtttatgaaa gatggacacc tttgtcaacg tagataatat caccgatgat
    21901 tccgctggaa agtgggctga ttaagaaagc acaagcatta ccgacttctt cgatcgtcac
    21961 accttgctga tcaggcgtac gagattcaga taactggatc agtttttgat agtctttaac
    22021 acctgtgaca gctaatgttt tgattgcccc agctgagatt ccattgaccc gaattccttt
    22081 aggcgagaac tcagctgcca aataacggat agcagcttca agagaagctt tcgcaatacc
    22141 catcatgtta taattcggga ttgcgcgttc tgaacctaga taagtcatgc taacgatccc
    22201 gctgtttggt gcaagaaacg gttgtgcgta ttttgataca gcaattaaag agtagctgct
    22261 gatatcttga gctaattgat agccgtctct ggaaatatca gacacatttc cggacaattc
    22321 ttctttgttg gcgtaagcaa ctgcatggac aaggccgtgg atctccccag catattcacc
    22381 gatggcattc attgcttttt cgatgctttc gtcgctagaa acatcacatt ctacagtgaa
    22441 tgcttcttcg ccgaccaatt tgactaatga ttttttcatt cgttcatttt ggtaagtata
    22501 gataatttct gccccttgtt tcaacattgc ttcagcacaa ccccaagcaa tgcttttttt
    22561 gttagcgacg cccataatga cgattttttt tccttctaga aatgacataa tgactctcct
    22621 ttgtttctga taaaaatttg atagtcgtaa aaaaatagag aataatagct aaaataactt
    22681 cttgatttct taattattag tcaaaatgtt ttgattgtca aactatttt
//
LOCUS       DADVXU010000043        22686 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.045, whole genome shotgun sequence.
ACCESSION   DADVXU010000043 DADVXU010000000
VERSION     DADVXU010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 22686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.045"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            67..3345
                     /locus_tag="KB953_001513"
     CDS             67..3345
                     /locus_tag="KB953_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HBB6866493.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3886..5793)
                     /locus_tag="KB953_001514"
     CDS             complement(3886..5793)
                     /locus_tag="KB953_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HBB6866494.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5923..6798
                     /locus_tag="KB953_001515"
     CDS             5923..6798
                     /locus_tag="KB953_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HBB6866495.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6878..7852
                     /locus_tag="KB953_001516"
     CDS             6878..7852
                     /locus_tag="KB953_001516"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBB6866496.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8032..9177
                     /locus_tag="KB953_001517"
     CDS             8032..9177
                     /locus_tag="KB953_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBB6866497.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9314..9658
                     /locus_tag="KB953_001518"
     CDS             9314..9658
                     /locus_tag="KB953_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HBB6866498.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            9929..11215
                     /gene="efmA"
                     /locus_tag="KB953_001519"
     CDS             9929..11215
                     /gene="efmA"
                     /locus_tag="KB953_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HBB6866499.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11353..11952)
                     /locus_tag="KB953_001520"
     CDS             complement(11353..11952)
                     /locus_tag="KB953_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HBB6866500.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12356..13477
                     /gene="mnmA"
                     /locus_tag="KB953_001521"
     CDS             12356..13477
                     /gene="mnmA"
                     /locus_tag="KB953_001521"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBB6866501.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13723..15144
                     /gene="arcD"
                     /locus_tag="KB953_001522"
     CDS             13723..15144
                     /gene="arcD"
                     /locus_tag="KB953_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HBB6866502.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15158..16276
                     /locus_tag="KB953_001523"
     CDS             15158..16276
                     /locus_tag="KB953_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HBB6866503.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16336..17628)
                     /locus_tag="KB953_001524"
     CDS             complement(16336..17628)
                     /locus_tag="KB953_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBB6866504.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17760..18338
                     /locus_tag="KB953_001525"
     CDS             17760..18338
                     /locus_tag="KB953_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBB6866505.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18467..18844
                     /locus_tag="KB953_001526"
     CDS             18467..18844
                     /locus_tag="KB953_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HBB6866506.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18777..20849
                     /locus_tag="KB953_001527"
     CDS             18777..20849
                     /locus_tag="KB953_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBB6866507.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21060..22007)
                     /locus_tag="KB953_001528"
     CDS             complement(21060..22007)
                     /locus_tag="KB953_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBB6866508.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22253..>22686
                     /locus_tag="KB953_001529"
     CDS             22253..>22686
                     /locus_tag="KB953_001529"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBB6866509.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQ"
BASE COUNT         7348 a         4142 c         4813 g         6383 t
ORIGIN      
        1 acagtaaaaa taaattaggt aatactttaa gtaaacgctt tctaatttgt tacgggagga
       61 atacacttgt taaaaaaacg tataaaatac agtgcgctaa tgatgaatct ccttatgctc
      121 agtaccccgg ttttaatttc tgtcgttgaa gttgctgcta acgaacaaca gtctgtgaac
      181 gaagaaaatg cacacgtgaa cattgaaggg atgtcaacaa tggaggaaat accaagaact
      241 agtgaagata tggatacgga agaaggaaat gatcctatga tgaaagaatc agaaaatgaa
      301 aagacaaaaa agcaatctga agaagcaaat gaagagtgga aaggggttgt tttcggagaa
      361 agcacaagtg ctagtaggta tgccattgaa ccattagaag acggcgtgcg gctctcttcc
      421 acgaataacg gcggaaaatt ccagtcaagc ggatcagatg gaatgaccta ttattatcaa
      481 gcaatcccta aagacatcaa ctttaggatg cgggcgacga tcgaaataga atcttggacc
      541 tatacgaatg cccaagaagg atttgcttta atggtaagag acgcagtacc aaaaaatcat
      601 ttgtttgggc aggcatttta ttcaaattca tttgccatac taggcagtcg gatcgaatat
      661 gtctgggact cggatagaca agaagtggtc aacacgagcg gcagcaaata tacgatgcga
      721 cttggattgg ggacacgggc aatcacagga atatcatctg aagatccgca agctgcgcca
      781 gctcctggga gtgtctcagt ggaaacaaca cctttagaga cttcagcagg gttcttggaa
      841 aaagaaacag gttcttataa tatattcgga aacggtcatg ggaatctgtt tccggccacg
      901 aactcgatta ctaaactgga cgtagagatg aaaaaaacga atactggttt agaggcatat
      961 tattacgatc ctgaaacagg agaagaaatg gcttctgaca tcatgtatga ctgggaaaaa
     1021 ttatacgctt cagatgaatc ggttatttat gcaggatttg ctgcagcaag gaatatgacg
     1081 atcaaggtag aaggaatcga agttgcttac tcagatcctg cgacagatcc tcctgggcaa
     1141 gaaagaccta ctcgcttgat tgatccgatt tacactgtga cctctagtaa tcattcagga
     1201 aaccaagatc atactatgtc ctttagagca aatgctgacg gattattgac aattaaaaat
     1261 aaagcaacag gagaggtgct taagaacgca aaaaacttgg ctatcactgc gaatcgggaa
     1321 tttgattatc agacaaaact gatggaagga acgaacgaat ttactttcag ttttacgcca
     1381 gataaaaact atccgccaga agaatacgta gagatgacta gttatgaaac aaaagagatt
     1441 cttcatacag tggactaccg taaacctaaa tattcgacag tttatgtaac accagaaggg
     1501 tcagatgcag gaaacggaag cagacaaaat ccattatctc tgacacaagc attgagatac
     1561 gcatatgctg ggcagacaat cgtcatggcg cctggaactt attccttcga aagaggattg
     1621 acgattccaa aaggcgttaa cgggacaaga gaaaacccga ttcagttgat tgcagagaaa
     1681 aatccagaaa acaagcgtcc tgtacttgat tttaaaggaa ctggaaatgg gatgacactg
     1741 ttcagtcact actggggttt acgagggttt gacattacaa atagtgcagc tatgcaaaaa
     1801 ggattacaga tttctggaaa tcataatttg atcgaagaga ttcatgctta tcggaatgga
     1861 aatacaggaa tccaaatcag cggtagctca aatgatcctt tcgaagcatg gcccgcgcat
     1921 aacttagtca agaactgtac atcttttgaa aatgccgatc caggatttga agatgccgat
     1981 ggatttgcgg ccaaactgac aataggagaa ggaaatgtat ttgacggttg tatcgcgtat
     2041 cataatgcag atgacggttg ggaccttttt gctaaaaatg aaacaggttc gatcggaaaa
     2101 gtaatcatca aaaactcagt tgcttatcga aacggctggg taccaggaat tgagggagaa
     2161 ggtaacggta atggattcaa aatgggcggt tcttctttaa cgggccctca tgaattgatc
     2221 aatagtttgt ctttcgaaaa tcttgcgaaa ggaatcgatt ccaatagcgg aacagatatt
     2281 atcgtcaata gcagtacaag ttttaataac ggttcatata atgtggctct ttatacctct
     2341 tcagcaggaa acacagacta ccatgcttct ggtatcttat ctttccgaac agagaatctt
     2401 gagatgagag agcagatccg cccattgaat caaaacgaag atcaaatcta ccactcgttg
     2461 aactactatt ggaatgaacg agagaaacaa tcagaaaata ctcttggaca taccatatca
     2521 aaagattgga tcgagtctct aaatacatgt tcgaaagaag gtcagcagcc tgttacgcgc
     2581 tatgaaaatg gaagtatcca tgttcatgga ctgatgcaga ttaaaccggg gaatcgtcaa
     2641 acagaaaagg aaagagtgga tggtcttcca ttatctgtag gtgctatttt tgacggagaa
     2701 acctcaccgt cttcggaata tccagagtat gcaatcgtac aagaaccaaa tcagacaaaa
     2761 ccaaatgaac aaaataatcc gattattata caagaagcaa atggtgtggc ggagaatgca
     2821 gaaggaaata tcattgaact gaagcctttt acagtttttg tgtcaacttt cacaccaaat
     2881 caaggaacgc ctgtctttga agatatgcgt tatttcccag aaaagatcca aatcgttgaa
     2941 acacagtcaa gtgctcaatt ttctaatcat accgctattt tttcagcacc ttcaaaagga
     3001 gaatatacgc tagaagtcac ttatcgttcg gaaatatttg atggggaaaa atggtctgct
     3061 catacagaat ctgttaccca gcaaaaagcg atcactgtgc gagaaactgc cgatgtgccc
     3121 ccgggaaatg acaaaccaga aagagaaaat ccaggaaatg aatcaccaaa tggaaaacca
     3181 aatggaaaac cttctcatcc ggataggaca gcaaaagaaa gtgaaaaaaa tggcactcaa
     3241 ggactcccaa aaacgggaga atttaataac ccgttgctag cattagctgg aggaattttg
     3301 ttaatcggtg tagttgttta tgtcatgaaa cagcgaaaaa aataagtgga atttctcctt
     3361 ttatgatgta aaacaattga ctgaggaaag taaaaataga aggaaatgag accaatagat
     3421 gtctcacttc cttctatctt tttatagatg aggttctcct tgctgctttt gattggctgt
     3481 tcttattact tgatgcagga cagctttttc acaatctctc cactaaacgg tgttcaatca
     3541 gctgaccctc ggtattagct caacaaacgg caaaatatga ggaactattt ttccgttttt
     3601 ttctctaata ctcaggtcag gacgctgatt tcacaacctc tcgataaacg gtgttcaaaa
     3661 gccagtttct cggtagtaag tcaaatttat gcaaaaatgt ggaaagccat tttcacaaaa
     3721 tttttcttac tacttgaaac taagcagctt tttcacaacc tctaatacat ggtattccag
     3781 aagtaacttc ttcgaaaaat aagccgaaat gtcacaaaaa tttgagaagc gattttccca
     3841 aattccttct tatttcttga agctgaccac ttctggcaca acctcttacc agattcgcac
     3901 tcttttttct ggcgctagat acatagcgtc ttgttccgtg ataccaaatg tttcatagaa
     3961 ttcgtcaagg ttttttggtt gcacgtttgc tcggagttta gcaggggcat ggacatcgat
     4021 ttgcagtaaa agttgttggt attctttttt tgcttttgta cgccaaatcg ttgcccaatt
     4081 gacaaagaat tcctcgatag aaagatctga ttccgttttt gctgcttcca atgcacagct
     4141 tagaccgcct gcatcagcaa tattttccga aacggttaat tttccgttta cttttccgtc
     4201 agcaaaaggc agaccatcga attcttcgat cattgcttgt gctttgtttt gaaattgtgt
     4261 aagatcttct tctgtccacc agttgttcaa gttgccatat tcgtcaaata aagcgccgtt
     4321 attatcaaat gcatgggaaa tctcatgagc gataacagct ccgattccac cataattagc
     4381 agagcttgat tgttctagac tgtaaaatgg tgcttgaagg atcgctgccg gaaagacgat
     4441 cacattccga aatggatgat aataagcatt gaccgtgtca gcactcattt cccattctga
     4501 acgatccact ggtttgttcc atttgctgaa acgatctttt aatgtgagtt tgctgaaaac
     4561 aagagtattt gaaagcaaag taccaccctg atctgcaggt actgttttga actggtcgaa
     4621 taatgctggg atcttatcgg ggtagcctac ttggatacct aagttattta attttatgac
     4681 ggctttttct tttgttgcat ccccaagcca gtcattttct tctagacgtt ttttataaac
     4741 ggcgatcatt tttttcacca tttgttctac gtctttttta gcagcttcgc caaaatattt
     4801 ttttccgtag taatcgccaa ctacttgatc gaattgtcca gaggcaaggt aatacgctgc
     4861 ttttttctga ggcattgctt cgtctgttcc agacaatacg cgagaataga ttccgctgac
     4921 ttggcggaat tcttctgata gataaccact aagagaacgt actgttttaa tgatcatcca
     4981 gcttttgaat aacggaaaat gagcttctgt caggatgtca ttcaaatgat cgaagtacac
     5041 tggatcagtt acgatcactt tatctggagt tgtcccaatc aaacccgtga caagtttttt
     5101 caagtccagt ttatctgtgt atttaataaa tgtatcaaaa gattgcggat tatacatttt
     5161 gctgtaatct gcgttttctt ctgcactttt tacatgagga gcaatcaatt tatcaaattg
     5221 cactgcttgt tccgtgattg cttctgcctg acttttttct ttgccggcaa gctctaataa
     5281 ctgaacagcc atatcaaaga aaacgcctat tagttgtgca ccgttgggat ggtctgatgc
     5341 ataatacgtc ttatctggaa ggaataaaga aggcggataa gcaaagaagg cgttcgtttg
     5401 cgcattcttc atatctgcat ctacatccag attaaatggc aatggtaatg tatccaacag
     5461 ccattctgga aattgcttat tcaattcgtc gaaagactgc aatgattcaa ctttttcgat
     5521 tataggttga agcggttgag catgcaactc atttcttcgg tcataatcgt ttgccagttg
     5581 ataatatgca aggaaatgtt tcattttttc tgaagacact ttttctggat gggcaagcag
     5641 atcgtctgtt tctcccatca aaagttggtc gatcccgtca actaaatctt ggaaaccgcc
     5701 tgtagctggt ttatctgcag ggatttttgc tgttttttgc cattcttcat tgactgcttc
     5761 atagaaatct tgtttcaatc gttctttgtt cattcatcga tcgctccttt tttgtttcgt
     5821 gtgcccatta tcttacaaaa tgaaggaatt aacaaataga acataaaaaa ttgaaaattc
     5881 gaacaatcaa actgatttgt ttttttcaat ccttcactta taatgaaaaa aaggaggcga
     5941 tcccaactga atcaagtagt ggataaacct ttatatttca aagtagataa aaaaattaaa
     6001 aaattagcca gcactcagca gctgcagtca agaaaaagta aattgctttt tttagctctg
     6061 gtttttgaag atcaaagtta tgtgattatc gatcaatcag gacatccggt agaatattct
     6121 ccagcaaaat atacttatca agagggtctt tcttgtaaaa aatggaagct gatcaatgaa
     6181 gagccaatcg agttatctag atggatcaac agaaaagaag atattcccgt tttgatcgaa
     6241 gaaaaaaggt caggaaagga actcgcaaat tgctgggtgg gattgccaga agaacgtttt
     6301 ttgaggtata agaaatgggc taccccctca ggctatcttt gtggtactta cgcggcagca
     6361 gttctccttg cctattatca agactatcgt aaggggtgga tgcttccaaa tgagattcgc
     6421 aagaaaaata cggctgattc gttggttttg acgaaggcat tgcgtagcca gatccaacca
     6481 ctaggacttc ctacgattcc ttttcaggtt agtacaggga tttctagttt tttaaagaag
     6541 aatggcaatc atgagcgtgc acgagcaact cttctaggtt catggcagcg agctacaaaa
     6601 cgtatacgtg aaggcaaacc tgtgatgatt ggtattttga aagtacttgg cagtacatat
     6661 ggcaatcatt gggtgacagc gtacgcttat tttgaaactg aaacaggaga acgttattac
     6721 aaagtacacg acaactgggg cgattatcat aaagtcattc ctgccagttg gagtaacgga
     6781 acagtttcac ttccttaaga atttgctgaa attaacagaa aatcttggaa aaatatgtta
     6841 aactatcgaa gaatagagaa gttttgggga ggactacatg agtaataagt atcaggatga
     6901 cacgttgaag atttttagtt tgaatggcaa ccgtccatta gctgaaaaaa tcgcgaaagt
     6961 gttcggaaca gaattaggta aaagcgtcgt gaagcaattt agtgatggcg agatttccat
     7021 caatatcgaa gaaagtattc gaggggatca tgtgtatatc gttcaatcaa cgaatgcacc
     7081 tgtcaatgat tactatatgg aactactgat catgatcgat gcaatgaagc gagcaagtgc
     7141 gaaaacgatc aatgtcgtat tgccttacta tgggtatgct aggcaagacc gcacggcgaa
     7201 acctcatgaa ccgatcactg cgaaattgat cgctaacttg atcgaagaag caggggcaac
     7261 tcgagtactg actttagatc tgcacacggt tcaagtacaa ggattctttg atattcctgt
     7321 agataattta tttacgatgc cgttgtttgc tcattattac cgtcaattag gtctgacggg
     7381 agatgacatc gtagttgttt cgccaaaaaa cagcggtgtt cagcgtgcac gtagtctttc
     7441 agaatatttg aacagcacat tagccatcgt agatcatgca gatgatgctt caactgaggg
     7501 tggttatgtg atcggtgatg ttcaaggaaa gacatgtatc atggtagatg atatcttgaa
     7561 cacaggagca acgcttgcac gagcagcaaa tgtattaaaa gaaaacggcg caaaagaagt
     7621 atatgcctgt gcgtcacatg gattattgtc cgatcatgcg aaaagtattt tagacaatgc
     7681 accaatcaaa gatatttgta tcactgattc tgttttcaca gaagaaaatc gtcatccaga
     7741 aaacttaaca atcgttacat gttcagaatt aatgggtgaa gcggtcaaac gcatccatga
     7801 aaatacaccg atgagtccgt tattccgtct ggaagaaaaa gaattcgaat aaacaaaaaa
     7861 aagagggaaa gaagttgtga cagaagtgag cagcttcaag gagctgcgac aagacttttg
     7921 tcacagctcc ttttttctat gcccatgaaa cgtcctctag acaaaaatga gctcttcttt
     7981 ggtttgttcg ctattttatg ctagaatgtg cctagcaata atttggaagg gatgaatgaa
     8041 gtgaaaacta tttatctgga tcatgcggca actaccccca tgcatccaca agtaattgaa
     8101 gcgatgacgg caatcatgca agacacgttt ggaaatcctt caagcataca tggatttggt
     8161 cgccacgcgc atgaaaaatt ggaaacagca agacaaatca ttgcagacag tctgcaagca
     8221 aaacctcacg agatcatctt taacagtggg ggaacagaag gggataatac agctatctta
     8281 gaaacagcgt tctcgcgtca aaaagaaggc agacatctaa tcactacagc tatcgaacat
     8341 cctgctgttc ttaataccat gaaatattta gagacaaagg ggtttgacgt tacatatctg
     8401 cctgtagatg aaaaagggaa tctgtcgatc gaagaggtca aaaaagcgtt acgagaagat
     8461 acgatcttgg tctctgtgat gtacgggaac aatgaaatcg gaaatttgat gccaatcaaa
     8521 gaaatcggtg aacttttagc ggatcatcca gcttatttcc atacagatgc ggtacaagcg
     8581 tacggaaacc aaacgatcca tccagaagaa ttaggaatcg atttattaag tatctctgct
     8641 cataaaatca atgggccaaa aggcgtcggt ttcttatata aacgagacgg agtaaatatc
     8701 cctgcattgc ttttaggcgg ggagcaggaa gaaaagcggc gtgcaggtac agaaaattta
     8761 gcagggatct ggggtatggc aaaagcagtt gaactgctgt cacctgaaga acgtgaagca
     8821 cgtaacaaca aatataaagg attccagcaa ttgattcttg atacattaga agcaaacgaa
     8881 atcccttatc aagtaaatgg tgatttaacg aataaactcc ctcatgtgtt gaatcttcgc
     8941 ttaattggcg aagcaaatga tttgctactg atgaagcttg atcttaatgg ggtcgctatt
     9001 tctactggtt cagcttgtac agcaggaaat atcgagcctt cacacgtatt agaagcaatg
     9061 tatggagaag atacaccgat actaaaagaa tccatccgta tcagctttgg tttgggaaat
     9121 accgaagacg acatccagta tttccttgat caattagttc gtgctgtcaa aaaataacta
     9181 gtgaagtcat tcttatatga acagatgtct gagagaacgc cttcattttt tagggaactt
     9241 aactgaaaaa cctagtaaaa atcaggataa acttttataa taaagagaaa ctaattttat
     9301 cgaggtgaaa atcatggcat ttgaaacaac agcaacggta ctgggcgcac caattagcta
     9361 tcgtttgcat cctgatgcaa aaagatatac attgcgcgat aatggattta cagaaacaaa
     9421 cggaggcaat tatcaattga tccgaccatt agatgccacg ccgcaaagca aagaaggatt
     9481 caaattaaaa atcactgtat caaaagatat tcaaacattg aaaatgtcga tcactacagc
     9541 aaatggattg aaagcagtca atattttcaa agatccaaaa cagaaaatga gccaagataa
     9601 attttatttt ctgatggatg ggatgattag ccgtgggtta ttcgaaaaag tcgaatgatc
     9661 tgtggatata aaaagacaac tagttttgaa acggcctatt gttagatgac taactttgta
     9721 aggacgacgc aaattagttg tcttttttat ttttaaattt aacacatgtt gttattttta
     9781 atattttgct gatttattgt ttctaagtaa agaaatttaa attgaaacga ttgttattta
     9841 gggaaaaagg tattttttga aaaaagaatc ccgtataatg attgttgtgc aaacgctata
     9901 caaaatgaaa aagaaaagag ggatgtttat ggaaaatgaa cagtcggtcg ttttaacgaa
     9961 ttggaagaga aattatttgt tctttttatc gggacagttt ttgtcaggaa tcactagtat
    10021 ggtcgtccaa tatgcgatca tctggtattt gacaagagaa accggttcgg cgacaatttt
    10081 gagttttgct actttactag gaatgattcc catggtttta ctaagcccat ttgtaggacc
    10141 gctagtggat cgctgggata aaaaagctct tttgattgtc acggatatta ttgtagcgat
    10201 tttcgcgctt attttagcag tagtcggaac gatttcagaa tccttcccga tttggctagt
    10261 ttttgtgtct ttgtttatgc gttcggtcgc acaaacattc caaatgccaa cgatccagtc
    10321 gattatgccg acaatcgttc catcttctca cataacaagg acgaatggac agttaggaat
    10381 ggtccaatca gcgaatttca tcattgcgcc agctctaggt gcggctttgt tttcagtcgt
    10441 tcctgtcaat taccttattt tattagatgt attgggggca gtttttggtg tcggcttatt
    10501 gatttttgtg aagatcccaa aagtttctcc tgaaatattg gaagttccgc ttactatttt
    10561 taaagatgca aaatttgggc tgcagcaatt gatggataat aaagggttat ggtacataac
    10621 gatcaatggt gcatttgtga tgctgttgtt catgccagca atcagtctgt atccattgat
    10681 gacgctagat tactttggtg gttccgttgg acaggcggga gcagtggaag tagtctacgc
    10741 agtcggaatg ctcttaggcg gtgcactgat cagttttata ggaacatgga aagaccgaat
    10801 gaaacctatt attatagcct acatcattat gggcctgacg atcggtgcaa gcggattggt
    10861 tccaaacgat agtcaagggt tcttgtactt tcttatcttg aatgcaggag caggttgtgc
    10921 aacgccatat ttcaatacac tgctgatggc aatgatccag cagagttatg aatcgaatgt
    10981 tttaggtcgt gtcttaggga acttcaactc tttgatgaac ctggcaggtc caattggatt
    11041 attatttgca ggaccattag ctgatcgttt aggggtagaa aaaatgttct tgttttccgg
    11101 aatcgggatt ttattatgcg gaatcgtttt atttttaacg tcagctgccc gaaattatga
    11161 taaagaatta cagaaaaaac tggtaaaaga acaccatgag caaaaagacg aatagtatgg
    11221 aatggagttg tgacagaagt cttgttacaa ctccatttct gatcctaatg ttcctgtggc
    11281 aataagccta ctcaaatgaa aaatatgagt aattatcttt atttgtttaa tacttgatat
    11341 agggcagttt tttcacgacc tatcgtactt cctctttgct tcatagattt ctttgatttg
    11401 ttgctcagat aacggagtgc caaatggtag tttttccttt aagagttctc tttccggtgc
    11461 gtctacgcct acattgacat cgttctcgat aggtacgctg taatgatgga tatgtccatg
    11521 aagattgcgg atagtgtcgt taggcccgag caacatcgga taatgagtca agtaatattg
    11581 ttgatggtcg aatttaacga tggcacctac atcataaaaa taaaatttgg gacttccgtc
    11641 agtaagccta ggatcatttt tttctaaata acggaaaaaa gcgcgactgt catggttccc
    11701 tttgatcaag tggattgttc cttgcagttg agtgaccagt tccaaaattt ctgcatttgc
    11761 tttttcatat tgcggatgca tagcaaggtc gcctagatga taaacatggt cggtttcttt
    11821 tattactgca ttccagctag tgatcaactg ttggttcatt tcatccacat ttgcaaagag
    11881 ccgcggagca aagtcgtttt ttcctaataa gcgttcatgg aaaaagtgca tatcactgat
    11941 aaagtaattc atgtatcttt tgcctccctt ttttcttaga gtaaacaaaa tcacttatct
    12001 gagcaactca taacatctaa aaagaaaagg ttgtaaatga gacgtgcagc tttaagcgat
    12061 aagaagcaat tttgaaaaat agtttttcac aatttataaa attattcctt attgccgaaa
    12121 ggctggccct ttttatgagt atgccaattt tttatatcga atgctcaaaa gatgagtgat
    12181 tttttggttg actatcatgt ttgtgtactg aataggtcca gtagaacagt atttttacga
    12241 cttgataatt aagggatgaa attttcagaa atatgacgta taatgtaaaa tactgaaaag
    12301 ttgcgacctt caactcgtag atgtttcaga atctaattga aatgatggtg atatcatgac
    12361 agacaacagc aaaacacgcg ttgtcgtagg gatgagcggc ggtgtcgact catctgtaac
    12421 agcacttttg ttaaaggaac aaggatacga cgtgatcgga atattcatga aaaattggga
    12481 tgacacggac gaaaacggtg tatgcacggc cacggaagat tataaggatg tagcgaaagt
    12541 agctgctcaa atcgggattc cttattactc tgtgaatttt gaaaaagaat attgggatcg
    12601 tgtatttgaa tatttcctag ccgaataccg cgcaggacgt acacctaacc cagacgtgat
    12661 gtgcaacaaa gaaatcaaat tcaaagcatt cttggattat gcaatggatc ttggcgccga
    12721 ttatgtagct actggacatt atgcgcaagt cactcgcgat gaaaacggcg tggtccacat
    12781 gcttcgtgga gtagacaata ataaagatca aacttacttc cttagccaat tatcacaaga
    12841 acagctttca aaaacgatgt tccctctagg cggcatggaa aaatcagaag tacgggcaat
    12901 tgctgaacgc gcaggccttg cgactgctaa gaaaaaagat tcaacgggaa tctgctttat
    12961 tggtgaaaag aacttcaaac aatttttgag caactatctg ccagcaaaaa aaggaaatat
    13021 ggtcactctt gatggtgaag tcaaaggaca acatgctggc ttgatgtatt atacgattgg
    13081 tcaacgtcaa ggactaggga tcggtggcgg aggagattcg caagaaccct ggtttgttgt
    13141 aggaaaagat ctagcgacaa acacattata tgtggggcaa gggttccatc atcctgcatt
    13201 gtatgccaca agcttggatg caagtgaaat ccactttacg acgaatgaac caatgccaaa
    13261 agaattcaag tgtacagcga aattccgtta tcgtcagcaa gacgttcctg tgacagttcg
    13321 cttattggat gataatcgtg cagaagtcgt attcgacgaa cctgtacgtg caatcactcc
    13381 tggacaagca gttgtatttt atgatggaat ggaatgtctg ggtggtggtt tgatcgacca
    13441 cgcatatcaa gaaacaaaag ttttacaata cgtttaagcg atattaaatg aatcaaaaca
    13501 gctatggcta acaaaagaaa caccttttct tttggatagc atagctgttt ttttgcaata
    13561 aaattaattt ttattcattt tatacttgaa atacttgtgt aaaaagttca taatatccaa
    13621 taatatgaat aaagttgctt tttgttaagc aagagcagtt gcaaaataga agggaatctt
    13681 ttgcaagtga tttgttcaat tttataagtg agggtgatgg aaatggaaaa gaaatcgacg
    13741 ggtattagca aatggggact ggttgctctg gttgtcagtt cgtcaatcgg aagcggtgtg
    13801 tttgggatca caagtgatct ggctagttct gcagctccag ggccagcgat cctttcatgg
    13861 ataatcgttg ggattggtat tttagctttg gtattatcgc tgaatcattt aggagaaaag
    13921 cgtccagatc tagatggtgg tattttcggt tacgcggaag cttcatttgg taaattaggt
    13981 ggcttcatca gtggttgggg ttactggttg tctgcgtggc tgggaaatgt tgcttttgca
    14041 acgatgctga tgagcgccat gggagaattt ttcccgatat tcaaaggtgg acaaaatgtt
    14101 ccttctattt tatttgcaag tatttttatt tggggtctga ctttattagt aaacaatgga
    14161 gttgaaggag cgagcgtcat caatacaatc gtgacgatct gtaaattagt gccattattc
    14221 ctatttttaa tatttgtatt tatcgcattt aaagtgaata tattcactgc tgatttttgg
    14281 ggtaacgtat ctgataatct agtgaatgga acagggcagc aaggaacgat ctggttgcag
    14341 attaaaggtt gtttgatggt tatgatgtgg gtctttgtag ggatcgaagg cgcaagtgtt
    14401 ttagccaacc gcgcgaagaa acgttcagaa gcccagcaag catcgatcat tggtctgctt
    14461 tgtttattgt ttatctatat ccttgcttcg atcttgccat acggcgtctt atctcaagaa
    14521 gagttagcaa ctatttcaca acctgctatg gcaaatattt taaaaggaat cgtgggaaat
    14581 tggggagcag cgctgatcaa tatcggcttg attatttcga tcattggttc ttggttgtct
    14641 tggacgatgc tcccagcaga aacaacgatg ttgatggctc gagacggtct gttgccgaaa
    14701 ggttggggaa aaacgaataa aaaaaatgcc ccaacttatt cgttagttgt tactgcaatc
    14761 ttgactaatc tattcttatt gactttctta gttactgatt atgcctatca atttgcctat
    14821 tctttatgta cagcggcaat tttgatttgt tatctacttg tcgggatcta tcaagtccag
    14881 ttttctttaa aagaaggaat caaaaaacaa gtagtgatcg gtgggattgc tgtagttttt
    14941 gaattgatgg gcatcatttt agcaggattt tcttatgtcc ttttatgtag tattgcctat
    15001 cttcctggtt tttgcttcta ctggatcgct tgtcgtgaaa ccaaacatca aattacggga
    15061 aaagaaaaag taatgattgg gctgatttgt gtggccgcac tgctttcaat cgggttgctt
    15121 gctgtaggct ggatcaagat ataaggggga aagaaacatg aaaatcgcaa atttcggtgt
    15181 agaagaatgg ctaaataaat gggaaaagaa agcggtatat gatattgcac aaagctcgat
    15241 cgaagcattg acattagacg aactcgttgg cttagatggg actagcgtaa gtaaattttt
    15301 cgaaaaacgc aaaaacgaac cattggacta tggctggatc gaaggatcag atgttttcaa
    15361 acaagaagtc gcttctcttt atcaaaccgt cgatccggaa aacatcttac aaacgaatgg
    15421 agcgactggt gctaatcttt tagctctata cgcgttagtg gaagcaggag accatgttgt
    15481 ttcgatgctt cctacttatc agcaattata tgacatcccc aaatctttag gtgccgctgt
    15541 tgactttgtt catttgaagg aagaagaaga ttggcagttt gatttagaac agctagaagc
    15601 cttagtcaaa cctgaaacaa aaatcatctg tttgaatagt gcaaacaatc ctacaggcac
    15661 attgctagat cggaaaactt tagaaaaaat cgcagaaatt gctcgtacag tagatgcata
    15721 tgtattgata gatgaagtat atgcgccttt aacggataaa ggtgaattct tgtcgatcgt
    15781 agatgtctat gaaaaaggta tagcaaccaa ttccctatcc aaaacctact ccattccagg
    15841 tattcgtatt ggctggacag ctactggacc tgagttggca gaagtttttc gaaagtatcg
    15901 agactacacg atgatttgcg gcggagtcct gtcagatgat cttgctgtac atgcattgaa
    15961 gaataaagaa aaaatattgg cgcgaaacaa aaagatcatt acagagaacc ttgtgatttt
    16021 aaaacaatgg gtagccaatg aaccgaaagt tgaacttgtc gctcccaatt acgtctctac
    16081 ttctttcatt aaattagcca tcgaagaaga tgaccagaca ttttgtatca atttattaga
    16141 agaaacagga gtactattag taccaggaag tgcctttgat ttaccgaagc atgcccgact
    16201 aggttactgt tgtaaaaaag aaaccttgga aaagggatta ttcctcttat ctgagttctt
    16261 gaaaaaacag gcctaaacaa aaacactgca aggacagtct gtgacaactg ttcaagcagt
    16321 gtttttcatt tacgcttatt gtctatgaga aggagagtgc cctgaagcta tggagactcc
    16381 tgaagctttt tcttctgcag tttcttgctg atggatctct tgtgtattgt gtcgtacaag
    16441 caagaagttg accaaaacaa gaagagaagt cgaaagaaag acgccgcgat aaccaaatgc
    16501 actggaaaca ctggagccga tcataggccc tactacgtta ccagttgctt ggaaagattg
    16561 gttataactg aagatccgtc cagcagcttg atgaggcgaa tattttgtga tcaatgcttg
    16621 gacagcaggg agcagacaag catcagagat accaatcaaa aaacgtaagg cagctaattg
    16681 ccaaacattc gtgacgaatg ccattgggat ataaaccaga atcgcaaaac ccaaaccaat
    16741 cgcaagtatt ctttcactcc caatcttgtc acctaagcga ccaaatcttg gagcggctat
    16801 caaagtggca atcccaggta tcgaagcaat gattccgctg atcaacgtaa cattcccatg
    16861 tccatgcagc aattggcgaa tgtacagact gatgattgga ctaatggaat tgttggatgc
    16921 ttggatgatc atcgttgtga cgaacatacc gatgacgacg tgtggatatt tcaagtcttt
    16981 gaaaatctgt ttggcagaaa ccatgttttc ttttttcact gggacaaatt tttcatggac
    17041 gaaaaacaga ctcaacaaga agacgaaaaa taagatgatc cctgtaatga aaaaggtagg
    17101 gcgatagcca aacgctgaag cagagatccc gccaagaagc ggtccaagta atgttcctgt
    17161 aacagaacca gttgctaagg ttccaagaac ttgaccactt ttttcttttg gcgtcccagt
    17221 tgcgacaagg gcagtggcat tactgatata accagaaaaa atcccttgaa gcagacgcaa
    17281 tgcgactaat tgatacacac tagtcacgca acccatcaaa gaaatgacga ttgccattcc
    17341 taatgaagca cgcaacagca tcaattttct tcctttttgg tcggctaatt ttccccacca
    17401 aggagagata agcgtagtca ccaagaaagt agatgaaaaa gtcaatccgc tccaaaagtt
    17461 caattgtgac gtactataat tacctaacgt atctatgtaa agtgacataa aaggcatgac
    17521 taaactaaat ccgataccag ccataaatgt tccaaaccat aagacaaaca agttttgttc
    17581 ccaagtttct cttttcctaa ataatttttc ctttattctt gcagccaatt agactccttc
    17641 tttctgaaaa ttttcatcta cagctcttta ttataaacta caatatgtag tttatcaatg
    17701 aaagtctctt ttttctcttt ctttttcgtg gtaaactgaa agagtgaagg atttatttta
    17761 tgagaggaaa catggaaatg aaaaaaagaa atctgtctca agagaaaatc atcgactgct
    17821 ttcgagaact agcagaagag atggatgttc agcaaataac ctttcagcat ttagccaaac
    17881 gcttggatat caaggctccc tcgctgtata accatttcaa aaatattcgt gatgtcaaaa
    17941 cagcgctaac agcgaaactt ctgcaggaac ttaatgacca gctgcgacgg gctcttgtgg
    18001 gaaaaagtgg tcaggaagca ctgcagatct atgcagaaac ttatcgatcc tttgcttttt
    18061 caaatcaagc agtgtatgag ttactgatca gtgtaccgca taccaatgaa gctgttttat
    18121 tagatggaat ccatgaaacg aatcagatca ttttgcagat atttgaggct tttcaattaa
    18181 cgaaagaaga aaaagttcat cgaagcaggg aacttcgtag tatgatccac ggttatcttt
    18241 ccttacgttt tttaggatac tttacaaaag aaccaacaat cgatccagaa gacagctatt
    18301 tttggatgat caataacttt atcgctactt tgccttaaac aatcagattc atttgtaaca
    18361 attttaagat gttcattaaa tgaccctttt ataagagaaa attcttgcaa ctttcttgaa
    18421 aagtgactaa gatgagtcta gaaaagctga aagaaggagc acgagtatgt atcaagtaat
    18481 taccatgttc ggggacaatg agccttggtg gttttttgaa gactgggaag aagatattga
    18541 agaagaagaa acattcgagt ctttcgaaga agcacgacaa gcgtacgaaa aacaatggaa
    18601 cgaaatccat caaaattatg aatatatcaa tgcaaaaagt aactttctca gcgccttttg
    18661 gaatgacgga gatgagcgct ggtgtgaaga gtgtgacgat gatttacagc aatacaaggg
    18721 acttgcgttg ttgaaaaatc atcagcccat cacagtagaa agcagaaagg aattttatga
    18781 aacaactaat tctagcggaa aaaccaagcg ttgcgaaaga cctaagcaag gtgcttggtg
    18841 ctaatcaaaa gcacaaaaat tattatgaag gacccaaggt gatcgttact tgggcccttg
    18901 gtcatttatt aggattgaaa atgccggaag atctgaataa ggaatggcag agctggcaaa
    18961 tggacacgtt gccaatgatc cctaaaaaac taggaatcaa accacttcct aaaacaggac
    19021 atcaattaaa agcaatcaag cagctggctc agcgaaaaga tgtctcagaa gcagtcattg
    19081 cgacagatgc cggaagagaa ggggaacttg ttgctcgctg gatcttagag tatgtgcatt
    19141 tcaataaacc agtgaagcgg ttatggatct cttctcaaac agataaagca atcaaaactg
    19201 gattcaagca actaagacct gcgaaagaat atgatgcatt gtatgaatct gctcttgccc
    19261 gggcaaaagc ggactggctg gtaggattga atgtgaccag agccttaact gtgaagtacg
    19321 cggataattt atcagcaggc cgtgtgcaga caccgacact agcattagtg agagaccaag
    19381 aaaagaaaat cgagcagttc cgtccacaaa cgtatttcac gatcacgttg caagctggaa
    19441 aagaacaagc caaaatgctt caaaaaaatc aatttgcttt aaaaacacaa gaagaagcac
    19501 aaaaacttgt tcaattgatc agtacaagca aaggaactgt cacagaagtc caagaaaaat
    19561 taaaaacgga atctgcgcca ttgccttatg atttaacaga gatccaaaga gaagccaatc
    19621 aacgctatgg ctattctgca aagaaaacat taagtcttgt acaaagtttg tatgagaccc
    19681 ataagatcgt tacttatccg cgtacagaca gcaaatatct gacaaatgac atgaaaagta
    19741 cgatgaaaga acgattgcaa gcagtcagcg actttgctcc tgaggtcaag cagtatttga
    19801 aaaacggagc catcgtccaa cagacaaaag tattccaaga cagtaaagtg accgatcacc
    19861 atgcactttt gccaactgaa aacagagcac gttatgaaaa attaagtaat gaagagcgaa
    19921 aaatctatca aatgattgtc tctcgttttt taggactgtt tgctcaaccc cataaagtca
    19981 gccaaacaaa agttacggtt gaatttggta aagaacgatt catttttcgg caaaatcgtg
    20041 tcgtccaagc aggatggaaa ggcgaaactg aaactgagac ggaaacagtc aaatgggaaa
    20101 aaggaatgag aatcaatccc gatttcgcca tcaaaaaaga actatctgca ccgcctaaac
    20161 cattgtcaga agcaagcttg cttggtcaaa tggaaaaata cagtctggga accccagcta
    20221 cgcgtgcaga gattatcgag aaactaatca aatctgaact gatggaacgt acccctcaag
    20281 gactgcaagt atctcctaaa ggtaagcaat tattagtatt ggtaaatccc tctttagtaa
    20341 caccagattt gacagaaagc tgggaaaaag accttgaagc tattgctgca ggaaagaaac
    20401 aagcaggaac cttcttgaat ggaatcgaaa aagaaacaaa acgcttggtg aatgagatca
    20461 aatcaagcaa acaagaatat caagatttct cgattaccca aaagaaatgc ccagaatgtg
    20521 gggccaatct gcgcgagaaa aacacacgtg acggcaaaat ctatgtctgc accaattcag
    20581 attgcaacta ccgcagaaga aaagatccaa aagtctctaa tcatcgatgc ccgcaatgtc
    20641 accggaaaat ggaaatcatc gaaggtaaaa acggtgctta tttccgttgt aaatacgatg
    20701 gaacgacaga aaaattgctt gataaaaaag agcagaagaa gaaaatgacc aaacatgaag
    20761 aacgacgctt gctgaaaaaa tatgcacaag cagaagaacc agaagaaagc ccattagcag
    20821 cagcactaaa agcagcaatg aagaagtaat aagatatgaa agaggttgtg atagaagtgg
    20881 tcagcttcaa gaaataagaa ggaatttacg aaaattgctt ctcaaatttt tgtgaaattt
    20941 cggcttattt ccgaagaagc tacttctgga acaccattta ttcggaaaaa aggactgtga
    21001 caagactttt gtcacagtcc tttttcattt aaaaaaatgt ttacttcatt tgacgtatat
    21061 tattggtgag gcttcaattc atttggcttc actttttctg tttctcttat gatataaaca
    21121 ggacgctgtt tcgtttctaa gaagattttc ccaatatatt tcccgatgat tccaagacat
    21181 aacaattgga ttccaccaat gaacaggaag atcgagacca tggaaggcca tcctgatgta
    21241 ggatcaccaa acaacaatgc ccgcaaaata atgaacaaaa ttgcaatacc agaaccgaca
    21301 caagacaaag cgccgataaa tgaagcaatc gttaacggtg catccgagaa gttcacgata
    21361 ccatcaatcg agtagttgaa taatttccag aaggaccaag atgtttcgcc agccacacgt
    21421 tcgcggtttt cataagaaat atattctgtt ttaaatccga cccagctaaa taatcctttt
    21481 gaaaatcggt tgtattctgt taattctagg acggcatcca ccatttgacg agtcatcaag
    21541 cggaaatcac gagcgccatc gaccatttct gtatcgctga ttttattgat caactggtag
    21601 aattttttcg caaagaaact gcgtatcgca ggctcgcctt cacgagtcga acgccgggtg
    21661 ccgacacaat cgatatcaga agtagtgatc cggttgatca tctcaggaag cagttcgggc
    21721 ggatcttgca gatcaacatc catcacggtc actaaatctc ctgtagaagc ttgcaatcca
    21781 gcgtacagag ccgcttcttt cccaaaattt ctggaaaatg agacaaagcg gacataagga
    21841 taagctgagg ctagcttctt gattgctgct aacgtaccgt cttttgatcc gtcgtcaata
    21901 aacaaatatt cgaactcatg ggcacctttg aatttttcaa cctcatcaaa aaaaagagga
    21961 acagtttttt cttcattaaa acatgggaca acaattgata ctttcatcgt ttctatcctt
    22021 tcaatcgtat gtttatatat ggtttacatt caaaatttaa taaaccttaa ataacacctt
    22081 taaaatttga ttttacgtaa aagaacaggt attctttttg taaactaacg tatttagttt
    22141 accatgtttc ctcggtttat caaactttta agaacaattt ttattagttg gcaagtagtt
    22201 ttggatagag aaaaaggagg aagttccagt ctattgacta ggagcaaaca aaatgaaaat
    22261 acacacacct atcggctttt tatggttaga tgcagatgaa tccggattga ctgctgtatc
    22321 ttttcagcca atttcagaaa gcgaatcagc gaatcagcga attttaacgc gcacacaaga
    22381 acagtttgaa gaatattttg cgggaaaaag aaaagaattt gatgttcctt tatctataaa
    22441 gagaggtacg ccttttcagc aaaatgtctg gaaagctttg tcgaccattc cttatggaga
    22501 aatccgaacg tataaagaag tagccgttgc tgttgatagt ccgaaagctg tgcgagcgat
    22561 tgggcaggca aatcgaacca atccattgcc aatcgttatt ccttgtcatc gagtgatcgg
    22621 gcaaaatgga aaactgacag gatatatggg aaacgcaata gatggggttg aaatcaaacg
    22681 ccaatt
//
LOCUS       DADVXU010000044        22463 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.046, whole genome shotgun sequence.
ACCESSION   DADVXU010000044 DADVXU010000000
VERSION     DADVXU010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 22463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.046"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            144..542
                     /locus_tag="KB953_001530"
     CDS             144..542
                     /locus_tag="KB953_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBB6866510.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            526..1220
                     /locus_tag="KB953_001531"
                     /pseudo
     CDS             526..1220
                     /locus_tag="KB953_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            complement(1814..2701)
                     /locus_tag="KB953_001532"
     CDS             complement(1814..2701)
                     /locus_tag="KB953_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBB6866511.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2886..3725
                     /locus_tag="KB953_001533"
     CDS             2886..3725
                     /locus_tag="KB953_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBB6866512.1"
                     /translation="MIRIIRLIGSVLIVFVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3921..4895
                     /gene="sgrA"
                     /locus_tag="KB953_001534"
     CDS             3921..4895
                     /gene="sgrA"
                     /locus_tag="KB953_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HBB6866513.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSEISTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5193..5549
                     /locus_tag="KB953_001535"
     CDS             5193..5549
                     /locus_tag="KB953_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866514.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5552..6709
                     /locus_tag="KB953_001536"
     CDS             5552..6709
                     /locus_tag="KB953_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6866515.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6735..7175)
                     /locus_tag="KB953_001537"
     CDS             complement(6735..7175)
                     /locus_tag="KB953_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HBB6866516.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7335..7841
                     /locus_tag="KB953_001538"
     CDS             7335..7841
                     /locus_tag="KB953_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBB6866517.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            8009..9922
                     /locus_tag="KB953_001539"
     CDS             8009..9922
                     /locus_tag="KB953_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HBB6866518.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            9927..10928
                     /locus_tag="KB953_001540"
     CDS             9927..10928
                     /locus_tag="KB953_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBB6866519.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(11130..11336)
                     /locus_tag="KB953_001541"
     CDS             complement(11130..11336)
                     /locus_tag="KB953_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HBB6866520.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11982..12830)
                     /locus_tag="KB953_001542"
     CDS             complement(11982..12830)
                     /locus_tag="KB953_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBB6866521.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12829..13896
                     /gene="gnd"
                     /locus_tag="KB953_001543"
     CDS             12829..13896
                     /gene="gnd"
                     /locus_tag="KB953_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HBB6866522.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            13898..14899
                     /locus_tag="KB953_001544"
     CDS             13898..14899
                     /locus_tag="KB953_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HBB6866523.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALIERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14896..15180
                     /locus_tag="KB953_001545"
     CDS             14896..15180
                     /locus_tag="KB953_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HBB6866524.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15213..16010
                     /locus_tag="KB953_001546"
     CDS             15213..16010
                     /locus_tag="KB953_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBB6866525.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            16024..16857
                     /locus_tag="KB953_001547"
     CDS             16024..16857
                     /locus_tag="KB953_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBB6866526.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16875..17732
                     /locus_tag="KB953_001548"
     CDS             16875..17732
                     /locus_tag="KB953_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBB6866527.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17754..18263
                     /locus_tag="KB953_001549"
     CDS             17754..18263
                     /locus_tag="KB953_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBB6866528.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18300..18701
                     /locus_tag="KB953_001550"
     CDS             18300..18701
                     /locus_tag="KB953_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866529.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18813..19166
                     /locus_tag="KB953_001551"
     CDS             18813..19166
                     /locus_tag="KB953_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866530.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19200..19643
                     /locus_tag="KB953_001552"
     CDS             19200..19643
                     /locus_tag="KB953_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBB6866531.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19660..20745
                     /gene="uxuA"
                     /locus_tag="KB953_001553"
     CDS             19660..20745
                     /gene="uxuA"
                     /locus_tag="KB953_001553"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBB6866532.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20738..21451
                     /locus_tag="KB953_001554"
     CDS             20738..21451
                     /locus_tag="KB953_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBB6866533.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(21658..21807)
                     /locus_tag="KB953_001555"
     CDS             complement(21658..21807)
                     /locus_tag="KB953_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866534.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            22203..22403
                     /locus_tag="KB953_001556"
     CDS             22203..22403
                     /locus_tag="KB953_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBB6866535.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         7746 a         3451 c         4641 g         6625 t
ORIGIN      
        1 tccatacatc cattgtactc tgagaaatca cacaatatcg aaacaaattc cgttaggaat
       61 gtgttattca ataaatttta taatctagaa acattttgaa taaaaactat tagacaaatg
      121 atattttacg aggaggaaca gagatgacga tctttgaaac ggttgtaaca gatgttggta
      181 tagaagcaga agtattcagg aaagaaaaaa taatgatttt atttggagat gaagcaccgg
      241 aaacattaac agattattgt tataagattg aattgaatca ggtaagaaaa gaaataactt
      301 gtgatatgaa gctctgtttt gataaacaaa tgtatgtaat cacagcagta ggagatgttg
      361 tccaaactaa tttgaatgaa ttaggacata tcacgataaa atttgatgga tctcatgagc
      421 cggaacttcc aggaacaatg tatgtagaag ataaggaaat gccgcattta agaaagggga
      481 caatcattaa aatcgatcaa gaggatcaga atgggggaaa gctatatgga aatcatgctt
      541 gatacagcaa atacaaaaag tatttattct tgtcaacagt ttttgaattt aacaggcgta
      601 acaacaaatc ctacaattct gaaaaaagaa ggagcgtcaa acttcttctc gcatatgaaa
      661 gaaatcaaaa aaataatcgg acagattcct atccatattc agacaatagg aagaacggaa
      721 gaagaaatga tagaggatgc tcgagttatc atagaagaac tgggaaaaga tacgtttatc
      781 aaggtgccgg tgaatcaagc aggccttcaa gcgatcaaaa agttgaaaaa agaaggttat
      841 ttgattaccg gtacagctat ttacacagaa ttacaagggt atttagctat taataatgga
      901 gcagattata ttgcacctta ttataacagg atgcaaaatc aaggcattga tgctatgaaa
      961 gtgattgatt cgttgcggac tgagatcgaa cgtacgcaaa gaccaacaaa aatattagca
     1021 gctagtttca aaaattgtga acaagtcact acagccattc gatctggagc tcagcgataa
     1081 cgttaggtaa agagctacta gatcaagtat tagagatgcc ctcgattgat aaagcagtga
     1141 ggggcttcca agaagattgg gtgcaaactt tccaagcaga aacgattggt gaactaaaaa
     1201 aggtcgctga ttacttctga atcaatttct ccataaaata aacgatagcc ttctcgaaaa
     1261 ctcgaggagg ctatctgacg attcaatatt cattaattta aagtttgggt gactattact
     1321 aactattact tatttctcat ttaaacgatg tattccctaa cctctaatac atccttgtaa
     1381 ctctgaagtt acgccagttt tgaataaatt tcataaaaac aaatgaaaag tgtcgcataa
     1441 aaatatcttt cgtgtttctc tagtataaca gaataagaag aacaagtcca gaagggatat
     1501 ttttagtctt ttttaaaata acttttcagt cgagtttgag tttatgatag ttgtttttga
     1561 agcgatacgg caagtgaaaa aatagggaag catgaaagaa tctacttaat ggcgcattga
     1621 ttcatgtgat agaaataaaa aactttttta aattatatcc tcatgattgt aacaaatcca
     1681 gtgatttgtt ttggtacaaa taaaaaaagg ccccgatgaa gttgtggcaa aagtggtttg
     1741 cctcaaaaat aagaaggaat ttccaaaaat cacttctcaa attttgccac atccccaatt
     1801 gggtcaaatt tgtttatttg aatatatttt ttacataagg atctttaata gttacattgt
     1861 cttttttcat cactttacgg attgttttat ccatgaattc cgtatcaaga gtttttgctt
     1921 cggtagcaat tttttctagc tcagatttgt atttgtcttt attagaacct ttatcctgtt
     1981 tttttaccat ttttactaga taatagcttt gctgataagt cgttggatct gttacaggaa
     2041 tgatatcaga aacttccccg ttttttagtt taaatgctgc tttttttact tcagtaggaa
     2101 tctctggatt ggttgaatca aaattcattt ttccatcagt ttcctttaat gttttatttt
     2161 ttccataagc ttgaacaagc ttggagaaat tttctccttc atttgcttct ttttttgctt
     2221 tatctgcttc ttctttagtc gaaaatagaa tgatctgtgt actaacttct ggatagaatt
     2281 ctttccaagc agtgtctaga tctttatctg ttaattttac attggctttc aaaccgtatt
     2341 ggaaagccag catctgttta aagagtttct tatatgtctc ttctgtttgg ccaactgctt
     2401 ttaattgttc cttgtagttt ggaccgagtt ttttgatttg ttcattatat tcttttgtta
     2461 cttcctttga agaaatctct tttccgtata catcatcaaa tgctttgaag acaatcattt
     2521 tttttaatag tgtttgatta tttgatttaa cttttgggtt atcataaaaa tcctgaactg
     2581 taagtgttcc acctttaaac gttgcaatcg tttcattgga cttgctacaa cctgagaata
     2641 gtaaaattgc acatgctgaa aggcacataa ttagtagttg ttttttcaaa aaatttccca
     2701 tcctttcctt attaaaaaca gtctaacaga agaattaggg agacagtctt tcattttcgt
     2761 aaggttgctg tagatattct tagttcgtaa aaatctattc tctctatgat ggagaacatt
     2821 tacatagttt tcacgagaca agaaagaaaa gggttgttaa acttaatata aaggagggat
     2881 gaaagatgat caggattatt cggttgattg gatctgtttt aatagtcttt gttggttttg
     2941 ttttaggaat tcttgtcaat aatgcacttg cggatatgtt tattggaaat agtgaatgga
     3001 ttggagcaat tgccggtacg gtcggacatt tagctgtttt tttactagct ttatttcttg
     3061 ctagtaaggt cgaaggaaaa agaatagaag attatggtgt ttctgttcga ggaaaagact
     3121 gggggtattt agcgggaggt ctcgttgttg gaattggagt gtttctgctt attactagtc
     3181 cattatattt gacgggggcg tatcggttag attcaggaaa cgcaaacatt gtgccgctga
     3241 taacgagttt tattttattt attgcagtgg gggcatcaga agagctttta ttccgtggct
     3301 tttttcagca tcagatgctt agatttggtc cgttaatagc aatgattgga tctgctgcat
     3361 tatttgctct tttgcacgga ttgaatccta acatgacatt tctggcagtt ttcaatatct
     3421 ttttagcagg ttgctttttc agcgcactta tctacagtac aggaagttta tttacggcta
     3481 ttggtgctca tattacttgg aattggacac aaggagctat tttgggtata ccagtcagcg
     3541 gaacgcaaga acaaggtttt ttctccactc tgatccaatc gcaaaataca ataattactg
     3601 ggggaaactt tggggctgaa gcatctatat caactacatt attcttgtta gtcttgattg
     3661 ttggtttgtt actctatgca tataaaacaa agcggatgaa gaaatacact gctgtttatc
     3721 aataaaagat gaagaaagca aggctgatat cggtcttgct tttttaagtc tgtcattgat
     3781 taaacgaaaa agtaaacaaa ttcaagcgaa tacacgacat cttttgttag attttcacaa
     3841 ctgactgtcg tgttctcatc attttcattt tatataattc ctttatcaaa tagaaggtaa
     3901 ttatatggag agtgatagaa atgaagaaaa cagcgaccgt attatttgtt attcttgggc
     3961 tgagtataat tgcagcaatg attggaatca gtacttctgc aaatgaacgg gcaaatgaga
     4021 atctttcatt tactgtgaaa actgatcgga ttgtttatga catgacacaa caagtcatta
     4081 ctattccagt caaaccaaac aaaagcgtga atgctagtga tgtacatgct gttctaacat
     4141 atggttggga tggtaatgga agttcagaaa aagttattgg ggaagtttat ttaaaagacg
     4201 tacaatggac cgctggtatt gagtatacaa taatgatttc tgcagaacta tcgattgatg
     4261 agatcaagag taaagataaa gttgatttga ttgtttttta tgatggacag atgacgatca
     4321 cggagaattt gaaaccaagc agttggacag ttgttggacc gccaagcagt acggatagca
     4381 caagtagtga aagcagtaca gaaaacacga gcggtgaaag cagtacagag agtacgagca
     4441 gtgaaattag cacagaaaac acgagcagcg aaagcagtac ggagagtacg agtagtgaaa
     4501 gtagcacagg aagcacgagc agcgaaagca gtacggagag tacgagtagt gaaagtagca
     4561 caggaagcat gagtagtgag agtagcacag gaagcacgag cagcgaaagc agcacaggaa
     4621 aagtaaataa tgaacttgat ttggtaccta tcataccaac taaggaacaa aagagaaatg
     4681 cagggagcaa cctaaaaagt gtgctaaaac agcctgattt tgtttctcgt ggaattgatc
     4741 atcaagaaaa agcgaaagaa acagataata gaaaaagcga tttaccagaa actggtagta
     4801 gaaccttgaa caagctaatt acatggatgg gtgtgttact cattttgatt gtaggagcaa
     4861 gctattttcg ttctctcttc catcgagtga aataaagaat aaaagcaaca gatctaaaat
     4921 tgaataaaac gatagatttt tgagtgaata tttcattcga gagtgctgta ttgcctggcg
     4981 aagttgtagc tttgccagat ctatttttgt ttactaacat atttgagaaa caggaaataa
     5041 caggaacaca aatggaaaat aagagcaaga ataattgaca atgcacactg cgcatggtag
     5101 tatctctgaa aaatcagtaa cgaatctatt tgtacgttat tcatgtagct gtcggaataa
     5161 gtctaaataa tagataaaaa ggagtatgta ctttgtttga agaatttatt gatatcaatg
     5221 aaaggcaagt atatcaattt ttgaactatt gttatgaaag agacgagaaa ttatatgtgg
     5281 tgaaggatat cgctttagat ctaaactaca ccctggctaa gatgaattcg gtgattcaac
     5341 aagcagaatc gttttgcgaa aggtatccag aatataaatt atcttttctt tcagaaaaca
     5401 agatgataaa agtcgagttt tcttctcagt ttttactgag taaagtttat tctatacttt
     5461 tagagggaac gattggttat attttactgg attcacttta caaaggtact tatcaatcct
     5521 tagaaaatct tagtcaaaaa attatataag tttgcgtacg attcaaagaa aattaagaga
     5581 acttaatact attttgaaaa actatcattt atcttgcagc ttgaaaaaag gtaatccgat
     5641 agaaggagaa gaataccgta tccgatactt ttttcacttg atgtattggc agtcattcga
     5701 cgaaaatcat tctgatatat tatcaagtat aaaaaataaa cgtcaactat tagaaaaatt
     5761 tgcgaaacat gcgccatata cgcgtagtat agatagaaag aaatggatca gcctactaag
     5821 aatttcgttg caacgaatga aacaaggcca ctcgattacg tatgtcccag aagagatgaa
     5881 caaatttgtg catccattga taacaaaaga agagtttacg cattacatac tagagccttt
     5941 ttttgaagca aacttctggt cgatgaatca catcactcca acagagcaag cttaccttta
     6001 ttttatgttt ggtgtaatga acacctattt ggaagaagat ttgttggata ctccagataa
     6061 agtacaagaa agttggggct ggattgaatc gtctattgta gaaaattttg aaaaaaactt
     6121 caagatttgt tttacaactt cagaaaaaaa ttatctatat gtcaatttgg caatgatcca
     6181 tttgtattca agggtttttg ggacaaaaaa gaaggtggat gcttttggaa aatcagcaga
     6241 agacagagat tttcagcata tatttccagt gatgtatcca atcatgcaac agttttttag
     6301 tggggtagct cgcagaattc cagagcttaa tacttattat gaaaagaaca gacggttgat
     6361 ttttcaatac tgtatgttag tcagggatat ttttataaaa cacgaacaac ctgtcatatt
     6421 ctatattcaa tcaaagtatg gtaaaacaca ggaattatgg gcaaagaaaa ggatattatc
     6481 tatgactgaa cgcccaataa aatatagtgc atccgctgat cagttacctg atattgtcat
     6541 tagcgattat cctatcgaca aaaaggcata tgatcaaacg aatacacaga tattttactg
     6601 gaatggacaa cctacaaaaa atgattgggc gcgtctgaaa gctgaaatca taaaaatcag
     6661 gaatgagaaa aatagatcca tattggaaaa attggatttc gttaattaag aaaagcagat
     6721 tgtttatgcc aaaattaaga cattgcaaaa agattatttt ctttaaaatc gacaagatga
     6781 ccagaaagca agagttcgtt gagctcttgc tttcttttag ttttagaaag gaacatcctg
     6841 attttatctt cgctaaatcc agtcaaaagt tttgcagatt ggtcgtagat aatcggtttt
     6901 ttcaataaag aaggggatgt acgaatctca atatatagtt cactcaaact gagttcatct
     6961 attctgtgaa caagattttt ataactaata gaatgagtag agagcatttc ggtcacgccg
     7021 ttgtttgtca attttagtaa gtggataaat tcatttttgg ttagtggttg acgaattaag
     7081 ttctgttcga taaacggtat atcatggacg ataagccaac gttttgcgcg tcttgaagac
     7141 gagcagccag aacctgtata caatttaatc atcatacatt cctccaatag ctaagtattt
     7201 atgaaataat aacatgaaga aatagtcgaa aagtgccatt tagaggtgta aaataagaaa
     7261 atgttgtcac gtataatgga actagctaga taagaaatac tattaaatca agaaaaaaga
     7321 aagaaggcac aagtatgtca aacacagccg acgcaatagt agtgatcgat ttacaaaaag
     7381 gggtatgttt tgattcttcg ccgatacacg aattagaaac agtgacagca ttagtgaata
     7441 aaagaatcaa aacatacgaa acgattggga aaccagttat tttcgttcaa cacgaagatg
     7501 ctcaattagt gccacaaaca aaaccatggg agctattgtc ggaattatct attcctgagc
     7561 acagttattt tgtgagaaaa acccatgcga attcatttta taagactaat ttgagtgttt
     7621 tattaactga attggactgt cattcgatcg aattatgtgg ggcacaagca gagtattgta
     7681 tagatacgac tgtgaagttt gctcatggat taggatacca gcttatgatg tgtagaggag
     7741 caacgacaac ttatgataat ccttttatga aagcaaagga aacaatcgct ttttacgaga
     7801 aaatttggga taaacgattt ctgacatttt tggagcaata agttgtttct tcgtttaaaa
     7861 aaagcagaat ggatactggt ttatccattc tgctttatta cgtaaaatga gaaaataatc
     7921 ctgttctatt tttatgggat aaaccattta ttttttgtat aaaaaatgtt tgcgtttaca
     7981 tataattctc ctagaaacat aaaaaggagt gaatagcttg agaacaaaac aagtattaaa
     8041 gggagcgttg gtcatcttat ttgctgtaag tacaagcgtc ccgcaatgga cagtatatgg
     8101 agagagcatt tcttccgaat cggaatctcg ggaggtagaa accacagaaa ctactgatgc
     8161 tggaaatgaa acgaaatcga gtacttcaca aacagaaaca aatactgaaa caacggttga
     8221 gacatctgtt actagcacag atcaagaaac gaccgctacc acagataacc aacaaacaga
     8281 agaagcaaca gctacaaaaa tgtcaaacga cgaatcctct tctgctaaag taagtgacaa
     8341 agagaatggg acagaaaatg atgatatcgg taaaggtgaa aatttcaatg gtaaagaata
     8401 tttgaattac ttcggtaaac agatgacaga aatacttaat ggaccaacag aagacatcaa
     8461 gtcaaaatta atagagttat tagtcactga gcttgctgtt aatgattatg gtgtagataa
     8521 agagattatc aaacatctga ttgctactta tctattgtac gatatcgctt ttgcaaaaaa
     8581 cagtgtgttg atgtcgatgc ttggaggtac agatgatggg acaacgccta cagaaaacac
     8641 aggcttctat ccaaatacaa aacaacttca tcaaactgta ctaaacaaac aagatggcaa
     8701 aatgatggat gtatatgcat attatgtcga tcaaggttca gataaaacag tcatgatcca
     8761 tggcggattt cgagggaatt ggaataacgg gatcgttaca gaagaataca atgattttta
     8821 taaggccggg tataatttat tatttgttga ttccagagcg actggtaata gcggaggcga
     8881 ttatgtgacc tatggacaat atgagtcaga tgacgtcctt tactggataa atcaagaagt
     8941 tcgagagcgg ccatctcaaa agattttact ttatggtggt tcgatgggag cagctacgat
     9001 gatgtctgta ttagcgaaag atattcctgt caatgtcaaa ggaattattg aaaattgcgg
     9061 atttgcatca attgatgaac aactacgttt tacgtacagc cagacagtag cccccgcact
     9121 accggatgcc ataaaaaatc agttagacat cattggggat caagagcatg aagatttatt
     9181 tatgggcctt ttgaaacaat actattttga tcaggagatg catttggata caaaagctgc
     9241 tctacctacg attggaatgt cagattcatt gcctaaattg attatccatg gaacagcaga
     9301 tgatgtagta cctgtgtcta acgcgcaaaa attatatgag ttatcaggag ggtataaaga
     9361 tcttttatta gtagaaggag cagggcatgg aaaggcacag gaagttgacc atgctgccta
     9421 cacgaaacat gtaacggact ttttgaaagt agtgttccat gatcagataa acgtaaagta
     9481 tgtggatgaa aacaataaaa gtctactcaa agaccaggac gaaatccagc tttacggagc
     9541 ttatggagaa aattatatga cagagcaaaa gacattcgaa ggatacgaat tagcaaatgt
     9601 tgaaggaccg actgaaggga tttttaatga gacgatacca actattattt tctattacaa
     9661 aaaaatccca gtagctccac caaaaaagca agatccgaca gagaatgcag acaataaggg
     9721 aaagaatgaa cctcgagagg atcaatcaag tgatgatacg caaccgattc agatgacagt
     9781 gaaagtacaa aaaaagacag atgcaaaaaa aagaatgttg ccgaaaactg gagaaaagaa
     9841 acattcgctt ctcatttatt caggtggatt tttatctgtt agcacgatta tcacatggat
     9901 ttggcgaaga aagagagggt aaatgaatga aatggttcaa gcgaatagtg attgcagtac
     9961 cagttatcat cgtagttatt ttagtcactg tatttttgat taatcaaatc acaccaaagc
    10021 cgattagctt gcttgttaga aaacaatttg acacaagtga aaaagaacaa gtatatgccc
    10081 gaccagatga tttccagcaa aaaatcgaac aaatcgatat aaaaaaagaa atatcctatc
    10141 cttcaaagta caaaaataat aatatggata tctacacacc gaaagtggaa aagaaaaaat
    10201 taccgattct tttctggatg catggaggcg catatgtagg aggggataaa aatgattgtc
    10261 gtgattattt ggaatatcta tgttcagaca cgcagcaaat catcgtaaat atagattatg
    10321 aacgatcacc ggaagcaaaa catcctacac cagttatcca attgaacgaa gccattcaag
    10381 cagctaaaaa agagataaaa gatcaagcag attggtcgaa tatttcgatt ggtggaaatt
    10441 cggcaggtgc acaaattagt ggagaatatt tgttagcact tcaaaatgaa gaaattaaaa
    10501 aagctgatca tcttgatccg actttggaga acaagcagat tacgaagttt atttctttgt
    10561 ctggtctgtt ggatccttct gcacttactc aagtaagtga taaaatctct agctttcttt
    10621 atgaaaaatg tggatgggct tactttgata ataaggactt tgaacaatca gagaaggtca
    10681 aaagtttagc cttggtcaga catgcggatc gttggacgca aaacacattc ttaacggatg
    10741 gaaatacaaa tacgtttacg aaacaaatgg aagaagtaac ttctgtattt gagaaagcgg
    10801 gggtgaaagt cacaaaagta gattatgcgg agaaaaatgc tgttttgaac catgaatatc
    10861 agtttgattt cagcaataaa caagcgcaag aaacatatca aaagttagta atgtttttca
    10921 aggagtaaag tcttatgatg caaaagaggt tgtaatctgt cacttagctc caagtcacaa
    10981 ggagcaattt caataaacaa gcttttcatg gtttatgaat attgtaactt gtgactgagg
    11041 agttagagat ggcaaggggc accttttatt cgaaatttgg gaatatcaca cgattattgt
    11101 cacgatctct tttacgtaaa ttttataagt cagatttgtt gttttgatcg tttaaaaaaa
    11161 gcaacaaatc cgaagcctgc ggaggcaaga agggacaaaa agccgagtgg taagcaaaag
    11221 aatgctggtt ccactaatat tccgttgtta ttgatataag gctgggaaca aagatgaata
    11281 gcaaataaaa tcaatgcact agcgaaaaaa agcaaagaaa tatggatagc tttcataagc
    11341 agcacctcct tttttataat tgtatgataa atagattaag aatctataaa tgtgacaagt
    11401 ttgttaggat gtgaataaaa tgatcatcta gcgaacttga aacaaagatg gctaatgata
    11461 agcaattaat gattcagcaa tcagataggt acaaagttta ccatcatacg tctagaccaa
    11521 taatagaaaa tatgggaata taatagaaaa gatttaggat acccccaaaa gaatttcccc
    11581 aaaaattcga tgtatcctaa acttttttat tttacataac aatcatatca actgtgaaat
    11641 aaaattacaa atgataacct aaaatgaggc caaaagccag tgttatcaag gaaatactta
    11701 attcaccaaa atttttttta attttttaga aaataagaaa caaaaataat aaaaaaaaga
    11761 aaagataaat tttttactac tgtatactaa ctttgttatc gcatctaaca agtaactcga
    11821 ataataccaa tacaaccttc tccaaagatg cgatgacata cgttctaacc caccgactat
    11881 tgatgttctg taccaccctt gcagaaccaa aaaagccact accaaatatt tggtagtggc
    11941 tttttttgtt ttatggattt ttcgcttttt ctgttatggt gctattcttt gatttcagaa
    12001 atagtatgtc gaactttagc gatagaacga cgtgattctt tttcgttgta aaacatcaaa
    12061 atcacatata gagagtccaa aatgcttagt tgagaaatac gggaagctaa agcttcagat
    12121 cggaattctg tttcttcaga aatagaaata aaaacgacat cgccaagttt agcaagggaa
    12181 gaattagctt ggctagtaat aacgattgtt tttgctccag tttttcttac ggtttcagca
    12241 atatggattg tttcttttga ttgccctgtg tgagaaatca aaacagcaca gtctttttcc
    12301 gtcaataaag aagcttccat caattgaata tggtaatccg tgctgtgaga agttgcgata
    12361 ggggaacgca aaaatttatg ataagcgtca gttgctacaa tctctgaacc gccaacaccg
    12421 aaaaaataga cacgtttaga ttcattgata atagtagccg ctttttttag atccttttgt
    12481 tgtaacaact tttttgtgtc atttaaagtt gaaatactag actcgaacac cttttgcgcc
    12541 atagtgagct cagtatctgt tttctgaata ttttcgtgga tagaaatagc agatagatca
    12601 ttttcttgga tcaacatggc cattttaaaa tctttaaatc cattataacc aagttttttt
    12661 gtaaattgaa aaaaagtaga gtcagcaatg cccagttcac ttgctaaatt gctaatagga
    12721 ctatgactta ctttgcttgg attttctaaa atataatcgg cgatagcctg ttcttttggg
    12781 ctgaagtcgt tgtacattgt tttgatttgt agtcttatgg agacgctcat gagaattcct
    12841 cattttcata ataagattat cattttttcc gttctcatta atgttaaaga tacaaggaga
    12901 gtaggaaaaa atcaaacaaa aaatattttt gtaaaatcta tttacaaaaa aatttttttg
    12961 tataatgagg gtataagaaa tggaggcgct atcatggaaa ttggtgtaat tggtttggga
    13021 aaaatgggat tgaatttagc tttaaactta aaagagcacc atcacaaagt acaaggactc
    13081 gacatttcaa gtacagctgt tagtcaggca agagaaaatg acattcctgc ttatcaagag
    13141 atctctgaat ttctagctac ttttgagcag aaaaaaatca tctggttaat gcttcctgct
    13201 ggagagataa ccgaaaacat gttaagaaaa ctttttccaa aactcggacc aggagacatt
    13261 attattgaag gcggaaactc ttattataaa gattcaatcc gtcgagcaga agagtttcag
    13321 aaaaatgaaa tcggttactt ggattgtggg acttctggag gaatcgaagg agcacgacat
    13381 caagcctgct taatgattgg aggagataaa gaaacttttc tatccgtgga acaattattc
    13441 aaagatgtag caattgaaag aggatatctg tatgctggtg catccggaag cggacacttc
    13501 ttgaaaatga tccataatgg aatcgaatat ggaatgatgc aagcaatcgg tgaaggattc
    13561 caacttgtac aaaaaagtga gtatgctttt gacttggaac aagtcgcacg tgtttggaat
    13621 catggttcgg tgattcgcgg ctggctgatg gaaatcatgg aagatcagtt aaacgaacat
    13681 cccaatttaa gtaattatcg gggaatcgtt tctgcatcag gtgaagcaaa atggacggtt
    13741 gaaacagcgc tagaaatggc tgttccagtt ccagtaattg caactagttt gttcatgagg
    13801 aatctttcac aggaagaaga tagtttttca gcaaaagtag tttcagcact aagaaatggg
    13861 tttggcggac atgaaatagt tgaaaagggt gagtaaaatg ccagattatc atatagcaat
    13921 cgttaattcc agcagtttcg gaagagtctt tccacaccac ttggaaagat taaagtcagt
    13981 tggagacgtt catttcttta ctgttgatca agagattcca ggaaaagaac tagcggagtt
    14041 acttaaagga ttcaatatca ttattgcaag tgttacgcca ttttttcgac gagatttttt
    14101 tgaaaacaaa gatgaactgc tattgatttc ccgacatggt attggttaca acaatattga
    14161 tttaaaagca gcaaaagaac accagacaat cgtatctatc atacctgctt taattgaacg
    14221 aaatgctgta gcagaaaata atataactaa tttgttgtcc atgcttcgac gcacgacaca
    14281 agcacaagca agagtcaagg acgatcgatg ggaagatcgg gcggagttcg tcggtaggac
    14341 gctgtttaac aaaacagttg gtgtgattgg tgcaggaaat accggaagcg gagtaatcga
    14401 aattttgcgt aacggttttc gctgtgatgt tctagcttat gatccttaca aatcagattt
    14461 atacatcgaa agctttggtg caaaaaaagt agaacttcaa gaattactgg aacaatcaga
    14521 tatcatttgt ttatgtgcga atttgacaga agagaactat cacatgattt ctgataaaga
    14581 agtcgatcag atgaaagaaa atgtttatct atctaatagt gcaagaggcg ctctagttga
    14641 tgaagaagcg atgattaatg gtctgaaaag cggaaagatc gcaggatatg ccacagatgt
    14701 tttagaagaa gaaccgggac gtcaagatca tccgtattta gcgtttgaac aagtagtgat
    14761 tacgccacat atatcggcct acacaaggga atgtttagaa gcaatgggtg aaaaatgtgt
    14821 cagagatgtc gaacagatca gtcagcacct tcttcctgaa cgttctgtcc agacaaagag
    14881 caactatata aaaggatgat gaagaaaatg gtgaacggtt taaaagtgaa aacaggccct
    14941 caattttatt tatatgaaga aggtggtatt tcaaaagttt ctgatctctt gaaatcatac
    15001 ggcgcgaaaa gagtactggt cacccacgga acggtatcat gggaaaaggc gctacctaaa
    15061 ttagtatttt tgaatgacga aacgattcaa tttttttatc atcgttattc tggagaatgt
    15121 agttatgctg aagccagacg aatagctaca atcatcaaaa aaatgaaatc gatttcgtag
    15181 ttggcgttgg cggaggaaaa ctgaccgatc tagtgggtta tagtgccaat cttgcaagaa
    15241 tcgatttcgg agtcatccct actttagcta gtaattgtgc tccatggaca cctcttgctg
    15301 tcatgtataa ggagaatgga caagcagaag ggaaaacaga acacttttac agacaagctg
    15361 ctttttttat aacagatccc caattagtga ttgattcccc tgttccatat ttcattgccg
    15421 ggttagcaga tacactagca aaatggtatg aatctgagct catcttagaa caagattttt
    15481 tgcaagatga atcatttttg aagctagcac aagatactgc gaagatctgc aaagaagaga
    15541 ttttgaatca ttctgctaaa gcaatccaag atatggcaca aagaaaactg acatcggagt
    15601 tcaaacagct ttcagagatt atctttgctg ttgctggttt agttggagga ttaggagata
    15661 aatacgcaag aaatgcggca gcacatgcta tgcatgacgc tatcagtaaa tatattccgg
    15721 aaagccatcg ttttctacat ggagaaaaag tagcgtatgg gattttctat caactggcac
    15781 tagaaggtcg ctgggaaaaa atagacgagt tatatccttt gtatcgggaa ttagaactgc
    15841 cggtttctct agaacaaatg ggattatttc cgaaagataa acagataata gataaaatgg
    15901 ctgaatttat tgattcgaaa gagaaagtgc atttgattcc tatagaggta acacagaaat
    15961 cactgataca gtcaatgatg gaactggaag gttatatcag aaagatctga aggaggagga
    16021 cacatggaaa cattaagtgt agtacaaagt ttgttgattg ctttatgggt agccgcggtt
    16081 atgtcccgct ggttaggtgg aggagccacg ttgaccttac gtttttctcc tttgatgact
    16141 ggattagtcg tcgggttgat catgggggaa gttgcacaag cgatgattgt tacagccgct
    16201 ttgcaaatga tttatatggg ggttttttct ccaggaggat caatgccagc tgaaccatct
    16261 attgcagcag ctatcgcagt accagtcgca ttattagggg atctgtcgcc agaagcagcg
    16321 attgccgttg cggtgccagt cggattgctc ggaagttact tgtatcagtt tcgttttttc
    16381 atcaatactt ttttaggtaa atatacagac aaagctgtag aagatttgaa tacaaaagct
    16441 atggcccgat caattattct ttatccaacg gttgcctcat ttttattatt cgttccgctt
    16501 gttttcattg ctttatattt aggggcgcca ttgatcgctg atgtaatcaa agctttagag
    16561 ggaacagtcg ttatccacat tcttgaagta gtaggtggag ggttagcggc aatcggtatc
    16621 gcgacaactg tatacgttat cggtcgaaaa gattatctgg tctttttctt ccttgcttat
    16681 tttatgagtg ttgttttgaa atctttagat attacgatgg ttacttatgc catttttggg
    16741 attattatcg ctttgatctt tgtccagtcg caaaaaggga aacaagtaca gcctgccaca
    16801 agcaataaca attcagcatc ttttgatgac gaagatgact atgatgacgg attttaattt
    16861 aggaggaatg ggaaatggct gaaataatgg ataatcaaac aggtgctcct gaagaaatca
    16921 cgaaaaaaga tgtaacaaaa gcctatctga gatggcattt tgcaaacgaa attcctcact
    16981 cttttgaacg ttacttggcg ccatcactac tatacggaat gatgccaatc ttgaaaaaac
    17041 tttataaaga tgaagactct ttaaaagcag catataaacg gcagttactg ttcttcaaca
    17101 ctcaattaag ctggggtggc ggtgtgatta ctggcctaat gtcttctatg gaacagcaaa
    17161 gagcagaaga agagtatgaa gaaaaagaga tcttgatgca ggatgacttg atgtataaca
    17221 cgaaagcggg attgatgggg gcactagcag gaatcggaga tgcaatcgat tcaggaaccg
    17281 tccaatatat ttttatcgca atcgcggtac cttgggcaca acagggaagt cctttaggcg
    17341 cactttttcc ttttatctgc tttgggttgt atcaggtttt actaggggta tttttcgcaa
    17401 gacaagcttt cactatgggc cgtaatgcta caggcctaat gcaaaacact ggtgttcaga
    17461 cagcgattga aatgctatca gttttagggt tgtttatgat gggggtgctt gcgggaaact
    17521 atgtaaaagt agaatcttca ttgaaatttg cgatatccgg acgtgagttt gttatccagg
    17581 aaattcttga tcaaatcgtg ccagggatat tacctttagc tgtcgtaatg ggtgtgtact
    17641 tgttctatac gaaaaaagga ttgaaagtca cacaagcatt attatggctg actggaatat
    17701 taattgtgct agcaggaatc ggtattttat aaaaagaagg gaacgtgaat aagatgggtg
    17761 tagtcaattt agcgcgtgtg gatgagcgtt tgatccatgg gcaagtaatg gtaacgttat
    17821 ctcaaaaaag tggagtgaat tccatttttg ttgtagacga agttgtagca aaagataaat
    17881 ttatgagaga tctttacaaa aatgcaggaa gccgtacagg acaaaaaaca attgtcatta
    17941 caccagagaa agctaaattc tattgggatg aatatcaatt taaagaatac aattgtattt
    18001 tgatcgcaaa aacagtccaa acgatttacg acttagtaaa acacggaatc cccatggaag
    18061 aattgaatat tggagggatt gctcaaaaaa atcctgatac agatctttta gtgacgaaat
    18121 ctgtttattt aaataaatca gatgctgaaa aattgaaaga aatgaatgaa caatatggcg
    18181 taaagaatat ttattttcaa gccactccat ctgcacctaa aacaggatta aaagatgtat
    18241 tgaacaagtt tgatttgtca taaacaaatg cttagaaaaa agataaagag ggcgaaaaaa
    18301 tgaagaagaa cgagctgttt cgtgattggg aatttcgtta tcgctatatt taccgtaagc
    18361 gccgtacaaa aaagagtaaa cagcgttttc tttcagcatt agtatcagat atttacagta
    18421 tgagaacaga tgtgacagtc attgcttacg atacgctagc ctaccgatca aaaaatattt
    18481 atgttggaga tatcgaaaaa gcagagaaag ttatctgtac ttattacgat acacccgttc
    18541 atgcattagg aagttatttc atgtttgatt ggaaagacca aaggaaaaaa acaatctact
    18601 ctatattatt gtccttcatt ttgttgttca gtttaggttg gtggggaatg atgatatata
    18661 acaaaaatcc tcatcacgta ttcgatctgc tatccgtata aacaggtatc actgtactag
    18721 catttgggag ctactttttc ttattgggaa aagcagctcg cggatggcct agcagacaaa
    18781 cttttattcg aaatacttca tccatattaa cgatgctaga aatgatccgt acgattgatg
    18841 atccgagtgt agcgtatgcg tttgttgatg aaggctgtta tggagaaaag ggcttagaca
    18901 gcgtgagatc gggtatgaaa aaagaggcta ttctttttta tctagactct gttggggcag
    18961 atacaccgct acagttttcg ggaaattatt tttcaaataa agaacagtgg ctgaaacaag
    19021 ttgataagtt gaaagaaaaa aacgtcaatt atatttttag tgcaagaaaa aagcaagcgc
    19081 agttcttcta tttgaccaaa acagatttac gagggaaaac atttaattgg caaaatgcca
    19141 atcagattat tgctcttttt cgataagtaa aaagtgcaaa acaaggagtg agaagactaa
    19201 tgttggaaat catgatcgtt acacatggta agttgagcga aggactgaaa gatgcggcgg
    19261 aagtaattgt cgggacatcg agccatattg aaacaatgtg cttagagtct ggggaggaca
    19321 tccaaacatt cggagagaag gtcaaagaaa aattaactat gatcaacaca gcagaaccca
    19381 tgctagtatt tgttgatcta attagcgcaa gtccgtacaa tcaagcgctc ttggcagtca
    19441 atgaactgac tgaagaaaag aaagcagcca cttatgtcat agggggcgtg aatctgccaa
    19501 tgttgttaga agcaatcaat catcagatgc ttcacacacc gatagaagaa gcggtggaac
    19561 aaattgtcaa acaaggaaaa gataatttaa ctgtttggca tgcttcagcg gaagaaaaaa
    19621 aattagatga ggacgatttt tagaaggaag gctgaaaaga tgaagtgggg atttagatgg
    19681 tatggtgaaa aaggggattc gatcccatta aaatatgttc gccaaatacc gggaataaat
    19741 gggataattg gaactttgct gactaaaatg cctggagatg tttgggagat ttcagaaatt
    19801 gaggagttga aagcatctgt tgagagccaa ggactggaac tgcttggaat tgaaagtgta
    19861 gcgatccacg atgcaatcaa ggcagggaca gatgaacgag attactatat tgataactat
    19921 atacgaacaa tcaaaaactt gtctcgatgt ggggttcgat tgatttgtta tagcttcaaa
    19981 ccaatttttg gatgggcgaa aactgatttg tttaatgaaa acaacgatgg aagctattct
    20041 cttttatacg atcagaaaat cgtcgatcag atggagccag gagaaatgta tcaattgata
    20101 cacagccagt caaagggatt caaattgccg ggttgggaag aagaacggtt gaaaaaattc
    20161 gctcagctgg aaaaactgta tgaaggaatg accgaagaag aattatttga aaatctgact
    20221 tattttttag aaaaagtgat tccagtgtgc gaaaaagcag atgtaaaaat ggctatccat
    20281 ccagatgatc cgccatggga actttttggt tttcctagag tgacgaaaaa tcttggcgac
    20341 ttgaagaaaa ttctaaatgc agtagattct ccatataatg gactaacgct gtgcacagga
    20401 tctttagggg caaatccgga aaacgattta gttgaaatca tccatgaggt aggttcgcgc
    20461 atccattttg tccacttcag aaacgtggag tttttaggag aaagaaagtt caaagaaacg
    20521 gcacatccct ctacagaagg ctcccttgat atgtatcata ttatgaaagc gttggtgcaa
    20581 gaagggttcg acggtcctat acgccctgat catggtagaa ccgtgtgggg tgaagtggct
    20641 atgcctggat atggcctgta tgatcgtgca atgggactca cttacatgca aggtctatat
    20701 gaggcgataa gtaaggaaaa accactggga gagcattatg aataaaatca agctcttcat
    20761 ctttgatatg gatggccttt tattagaaac tggacgttta gcttatcgtg cttatgtaaa
    20821 atcagcacaa aagtatgatt atgaaatgcg taaggaagtc tattatttac taactggcca
    20881 aacagaaatg gcgataagag aacaaatggg cattttgtac ggagaagatg ttccatatat
    20941 caaatggcga gaagcaatca atcaatataa agaaaaaatc gtgaaagaag ataagagagt
    21001 atatacgaaa aaaggagcag aagaaatttt atcttttgca aaagaaagag gaatccatac
    21061 aatcgtggct tcgtctaata cacgggtaaa agtagaaatg tatttaaaaa tggaaaatct
    21121 atatgccttg tttgatcaaa tcatttcagg ggatgatgta aaaaaaggaa agccggaacc
    21181 agagatcttt ttgaaagctt gttcaaaaat gaatattcct ccaagtgaag cacttgtatt
    21241 tgaagactcg attgcaggga tcgaagcagc aaggcgagcg ggcattttgt cttttctcat
    21301 caaagatcat ttgatagggt tgcccgatca taatgggaag cataagttga aagtagatgt
    21361 aagtttatat gaatccaata aagcagacta tgaattttat gacctgcatc aagcaaaacg
    21421 atttttagaa agtaaagggt tgtatctcta ataaagagga ctattttcta gtcctcttta
    21481 tttattgtcc tgcacgactg agggaacaga tgaaaaagaa gtaatttttt attttgaagt
    21541 atctacctta tttgtagtga acaatttaga acaaacacaa aacgcatcta ctgttatcta
    21601 ccagtagatg cgtttaaaga gaagaacgag aatcgtccat atgagggttt ccactcctta
    21661 tgcagatttt tttaattctt gcactgcttg ttcgatcgta acacctgtcg ctacatgatt
    21721 ggtattttct ttgtcaaaaa cgacaaatag attcaaatct gtatccaagg ttactcgata
    21781 ttgtatatct ttgttataga atgtcatagc cattctcctt tctttaaata cttgctgaaa
    21841 ggcaacgaac gcgttgataa cgaaattcca tttataagta taccaccaat tgaaaggttt
    21901 tcatagttaa gaaattttaa agtcaaaagt tgcagataaa agcaaataaa caagaaaaca
    21961 aaaagtgaat caatcgcggt tattcaactt tttcttcgat tcgaagacaa tagaagacaa
    22021 tgaattttat atttcattgc attatccttg tgttattaga aaaagcatga tatattaaat
    22081 gagtaatcta ttttgaaacg taatttgagc gatatatcta catagataaa actccttttg
    22141 cctatcaaat atttattgca acgaaaaaac gtacattgat acgtatcagg aggaatttat
    22201 ttatgaataa cggtacagta aaatggttca acgcagacaa aggttttggc tttatcactg
    22261 gtgaagatgg aaatgatgta tttgcacatt tttcagctat ccaaggtgac ggcttcaaaa
    22321 ctttagaaga aggccaagca gtgtcttacg atgttgaaga tggtcaacgt ggccctcaag
    22381 caacaaacat tgttaaagct taataaaaat tgacttttaa ccgtcttttc gaaagaaaag
    22441 acggtttttt tgtacgaaaa aat
//
LOCUS       DADVXU010000045        22204 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.047, whole genome shotgun sequence.
ACCESSION   DADVXU010000045 DADVXU010000000
VERSION     DADVXU010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 22204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22204
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.047"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            269..2104
                     /gene="tdc"
                     /locus_tag="KB953_001557"
     CDS             269..2104
                     /gene="tdc"
                     /locus_tag="KB953_001557"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HBB6866536.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2409..3887
                     /locus_tag="KB953_001558"
     CDS             2409..3887
                     /locus_tag="KB953_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBB6866537.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3853..5184
                     /locus_tag="KB953_001559"
     CDS             3853..5184
                     /locus_tag="KB953_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBB6866538.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5298..7850
                     /locus_tag="KB953_001560"
     CDS             5298..7850
                     /locus_tag="KB953_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HBB6866539.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7857..9296)
                     /locus_tag="KB953_001561"
     CDS             complement(7857..9296)
                     /locus_tag="KB953_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6866540.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9642..10004
                     /locus_tag="KB953_001562"
     CDS             9642..10004
                     /locus_tag="KB953_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBB6866541.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(10191..11048)
                     /locus_tag="KB953_001563"
     CDS             complement(10191..11048)
                     /locus_tag="KB953_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBB6866542.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            11168..12079
                     /locus_tag="KB953_001564"
     CDS             11168..12079
                     /locus_tag="KB953_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBB6866543.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            12086..13737
                     /gene="mdcA"
                     /locus_tag="KB953_001565"
                     /pseudo
     CDS             12086..13737
                     /gene="mdcA"
                     /locus_tag="KB953_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
     gene            13839..14138
                     /locus_tag="KB953_001566"
     CDS             13839..14138
                     /locus_tag="KB953_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HBB6866544.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            14128..15804
                     /gene="mdcD"
                     /locus_tag="KB953_001567"
     CDS             14128..15804
                     /gene="mdcD"
                     /locus_tag="KB953_001567"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HBB6866545.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            15808..16401
                     /locus_tag="KB953_001568"
     CDS             15808..16401
                     /locus_tag="KB953_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HBB6866546.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            16425..17501
                     /locus_tag="KB953_001569"
     CDS             16425..17501
                     /locus_tag="KB953_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBB6866547.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            17526..18395
                     /gene="mdcB"
                     /locus_tag="KB953_001570"
     CDS             17526..18395
                     /gene="mdcB"
                     /locus_tag="KB953_001570"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HBB6866548.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            18506..19270
                     /locus_tag="KB953_001571"
     CDS             18506..19270
                     /locus_tag="KB953_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HBB6866549.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            19759..21852
                     /locus_tag="KB953_001572"
     CDS             19759..21852
                     /locus_tag="KB953_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBB6866550.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            21877..22179
                     /locus_tag="KB953_001573"
     CDS             21877..22179
                     /locus_tag="KB953_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBB6866551.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
BASE COUNT         7188 a         3790 c         4731 g         6495 t
ORIGIN      
        1 tttagtgaat gaaggacagt cattttattt gtttgttcgc tttttttagg ttatctattt
       61 tagaagactg atttattgaa aaattgttcg cttttttgcg ttgaattatc agaaaaatcc
      121 gactttattt atttttgttt gtaaaaatct acgtggattt gatgttataa aaaaaaaatt
      181 ctcatttttg gcgctatagt gaagaggtgt tacagataaa cacatgtatc tgcaagacat
      241 tgaccaacaa aatatggagg gaagaaaaat gaaagatatg gatatcaagg ccgtctttat
      301 aggcgacaaa gctgaaaatg gaccagttta taaaatgttg ttgaacaaga tggtagatga
      361 acatcttgga tggagagaaa attatattcc ttcagatatg cctgcaatta gcgaaggaga
      421 taagctcact ccagattatc tagccacccg cgaccatatg atagaggtct tggatgaagt
      481 cagtcagcga ttacgcgcag gatcgatccc atggcattca gcaggccgtt actggggaca
      541 aatgaatgct gaaacattga tgcctgcttt attagcttac aactatgcga tgctgtggaa
      601 cccaaataat gttgcattgg aatcttctat ggcaacttcg caaatggaag cagaagtagg
      661 acaggatttt gcttcattat tcaatatgac agacggatgg ggacacattg cagcagatgg
      721 ttctattgct aatcttgaag gattatggta cgcccgttgc atcaaatcga ttcctttagc
      781 tgtcaaggaa gtgctgcctg aaaaagtgaa aaaaatgtct gagtgggaac ttttgaattt
      841 atctgttgaa gaaattttgg aaatgaccga aagctttacg gacgaagaaa tggatgaggt
      901 caaagcagct tcttcccgta gcggaaaaaa tatccaaaga ttagggaaat ggctggtccc
      961 tcaaacaaaa cactattctt ggatgaaggc gttagatatt tgcggagtcg gcttagatca
     1021 gatggttgct atcccagttc aagaggatta tcgaatggat attaatgctt tggaaaaaac
     1081 aattcgtgaa ttagctggac aaaaaatccc aattctaggt gtggttgctg tcgtaggaac
     1141 gacagaagaa ggtcaagtag acagtgtaga taaaatcgtc caattaagag aaaggttaaa
     1201 agatgaaggg atctatttct atttacatgt tgatgccgca tatggcggct atgcgcgttc
     1261 actgtttcta aacgaagcag gagaatttgt gccgtatgct tccttagctg aattctttga
     1321 agaacatcat gttttccacc actgcgtaac gattgacaaa gaggtatatg aagggttcag
     1381 agctatttca gaagcggatt ctgtcacgat agatcctcat aagatgggct atgtccctta
     1441 tgctgctgga ggaatcgtaa tcaaacataa aaatatgaga aatatcattt cttatttcgc
     1501 gccatatgtg tttgaaaaat cagtaaaggc tccagatatg ttaggagctt atattttgga
     1561 aggatcaaaa gctggagcaa ccgccgccgc agtctggaca gcacatcgtg tattgccatt
     1621 gaatgtgacc ggatatggtc aattgattgg ggcttcaatc gaggcagctc aaagattcag
     1681 agaatttctc gatcatctga cttttactgt taaaggaaaa acaatcgaag tctatccatt
     1741 gaatcatcct gattttaata tggtcaattg ggtattcaaa gaacaaggct gtacagattt
     1801 gaacgctatc aatgaattaa atgaaaaaat gttcgatcgg tcttcttata tggatggcga
     1861 tgtttatggt gaacggttta ttacttctca tactacattt acacaagaag attacggtga
     1921 ttctccgatt cgttttgttg aaagaatggg tctaacgaaa gaagaatgga aaaaagaaca
     1981 gaaaatcact ctgctacgtg cggctatcat gactccttat ttgaatgatg atcgaatttt
     2041 caatttttat acaaaaaaaa tcgccaaagc aatggaaaag aaactaaacg aaatcatcca
     2101 atagaactga tttttgtgtg aaagaataac aagagacata tggagaacag acaattattt
     2161 gcttcatcaa aacaattgta tttccccctg tttttcatat attcctcgcc aagatattct
     2221 tttttataaa acgttctata agttggatcg ttcgtttcat gtatcaaacc cagttcacta
     2281 agatcgttca atggatgttt cggtgatact gggttttgta ctgaatcgat gaaaccttgt
     2341 catttctgtt cttgaacaaa aataaaagaa aacaaaaaca tatgtcgcta ttgaaagaag
     2401 gaataaagat gaaagatact ggttcatcaa agcaaatcac gaccatgacc tttatcggaa
     2461 tgacctgcgc ccttgtagca agtgtacgaa atattccaga tgtagcagca acgagctgga
     2521 cgatgatttt ttacatgctg gttgcagtat tattgtacgc ttttccaatc tcattgatct
     2581 ctggtgaatt tgctggaatg tttccccaaa aaggtggtcc agaactttgg gtatcgaatg
     2641 ctctaggaaa aaaatggggg ttcgttgtct catggctgtt atgggtccaa atgtttcctg
     2701 ggatggtgat ggtcgcttca gcactcgctc cgttgtttgg taatattatc gataatgtgc
     2761 cgcttggttt gaacagcaaa tttacattag ttgtcatttt agtcgtttat tggatcatca
     2821 cttttttgaa tttgaaattt gatatggcaa aaatcggcgg aaaagtcggt gtttggttag
     2881 gattgtacgt gcctttgacg atgatgctct tattagggtt tgctgcgtgg atcaaagttg
     2941 ggatcgtacc aaccggaaca ttaggcagtt tttcatggga caaattaata ccagatacta
     3001 cgactgcatc ctcatttgtt tattttgcgc cgatcatgtt tatttttact ggtatcgaaa
     3061 tgtcctctgt gtatatcact cgattggaaa atccagttaa aacgtatatt cgcggagtat
     3121 ttgccgcgtt aatttttata tttttcgtca atattttgaa tgctttagtt gtagcaaatg
     3181 tcgtaccaaa aggacaaatg gaattgaata atatcgcgca atctatttcg atttattgcc
     3241 aaattcttgg tttgccacat cttattgtga atctgtttag tttacttgtc ttcattggtg
     3301 tagcagtcca attatctgct tgggcctcag gaccagcaaa gacagtgaca gaaagtgcta
     3361 gaaaaggtgc gtatccacca aaattcaatt tttggaagac gaatcaattt gacgtatcca
     3421 aatccgttat tttgactcag tctgtcatta tctcaatctt tgcacttttt tatttgctga
     3481 ttcctggcgt caatcaagca tttttgatgc tcgtaaattc aactactgtt atctactgta
     3541 tcgtctatgt catcatgggg atcgcagttt tgagattgcg ttatacacat gcaaagttga
     3601 atcgaccatt tagaattgga aaaaaagaga aaaagagcaa tcttggagtt tggactgtcg
     3661 ttattgtttt atttgcggca atcggatttt ctgtcggact gacgatgaaa gctggaacat
     3721 ggatcaatct aattgcagtc acagccatca gtgtcatttt gtttgtagta ccactctgta
     3781 ttgaaaaaat aaagaaacct tcttgggaac aggaagtcgc catgctgctt gaaaaagaaa
     3841 aaggtgaact tgatggaaaa accggcaatg aaacggactc taactaatcg aaatatccag
     3901 ttaatcgcat taggtggcgc aattggaaca ggattatttt taggttcagc aaaggcaatt
     3961 gcatcagcag ggccagctgt tattctcgtt tatttgatat gcggtttggc tatttatggt
     4021 ttgatgcggg ccatgggtga tttattttta gcaaatagta catttaactc tatcagtgat
     4081 ttcgttgact attatttagg caaaaagtgg gcattttttg tcaattggac ttattggctt
     4141 tgctggcttg gagctggact tgcggaatta acagcaatag gattgtatat ccatttctgg
     4201 tttgtacatg taccagcttt gataagcgga ggtagcgcac taatgatctt aatggcagtc
     4261 aaccttgttg cagtcaagtg gtttggtgaa ttggaatctt ttttctcaat catcaagatc
     4321 ttggggattt tcctttttat cttattgggt ataggattga gtgtctcaga ttttcatacc
     4381 cagtctatcg gaatcaaaga aaattttgga cagatcgatg aattactacc gtttggcttt
     4441 gctggaatgg tagtctcatt tcctatggtt ttattcagtt ttgctggaat tgaaatgatc
     4501 ggcttaaccg ttggcgagac aagtgatccg caaaagaatt tgaaaaaagc aatcaactgt
     4561 ctgccttggc gtattctctt tttctatata ggaacgattt tgtcgatttt atttgttgtt
     4621 ccatggcatt gtttagattt aaaagaaagt cctttagtga ctatgctgca agtgatccat
     4681 tgccaaggcg gagcagcaat tgtcaatcta atgattttgg ttgcttcttt gtcctcagca
     4741 aatagtgcag tctacagtac tagcaggatt ttatatcaaa cagcaaaaga aaaaagtttg
     4801 ccccattggt tttgtgcgct ttctaagaaa ggcgtacctt tacacggtat tttaattaca
     4861 ggaatgttat tttttgccgg tctttgtcta catttttttg tagctgatag tcgattgttt
     4921 tttcagcttt tggcaggcat gacaacgagt tgttttctat ttgtgtggtc ttcgatcttg
     4981 tgtgcacatg ttcaatttat taaagaaaag aaacggaaca ctacacaaag atataaagat
     5041 cttgctttgt tggtgttttt tacttcaatt gctttcagct tgttattcga aagcacgaca
     5101 cgctttattc cttttttatt ggttatctgg tttgtattct taaattttgt atataaaaga
     5161 ataagcaata gaaatatcag ttaggcccag atacattttg caaaacgaat tatttcagac
     5221 ccccctgaaa taatcgatac gcctgaaaaa acttccctgc gtataataac agtatcaatt
     5281 aaacaggagg ggataggatg aactggtaca gattgacggt agaagatgta ttgaaaaact
     5341 atcaaacatc aacagaaggc ttgtcgcaag aagaacgaca gaaacgcctg actgatcaag
     5401 gatataatga aataaaagca aggcagcaga caaaacgatg gaaaaaaata gcaaagcatt
     5461 ttactgatct gctgatgatc gtgttgatca ttgcctcttt tttgaaattt gcttctagtg
     5521 aatatattga aggaagtatt attttattcg tagtgatcgt caatggactc gttagttact
     5581 ggcaagaaag aaaagcggaa gaatcattga atggtttgaa gcagctgatg ggacaagaag
     5641 cgatagtact atcaaacggg atgcaagaaa aaatccctgc gaaagaactg gtaaaaggag
     5701 atgtcattgt cttacgtgcc ggagatgtcg ttccagcaga tatccgatta atcgaagcac
     5761 atgatttatt agtggaagaa tcgattttga cgggagaatc agaagcatct gaaaaaagtc
     5821 atgccgtttt gacagaagaa gaatcgatcg gcgaccagaa aaatatgggt ttttcaggaa
     5881 cgcttgtaca atctggttct gcagtcggtg tcgttgtaga aacaggaaac acgacagaaa
     5941 tcggtaaaat caatcaagca ttgcaatcgg tcgaacaaca aacgacgcct ctgattaaaa
     6001 aaatcaaccg attaaacaaa caaatttttc aaggcattct ttgtttgagc ctattcttag
     6061 ttatttttac gacatttcga tatggtatgg attggcatat ccttctttcg gctacgattg
     6121 ctttggtggt gtcaatggta cccgaaggat tgccagctgt tttgaccatg attctatccg
     6181 ttggagtaca tgaaatggca aaagaaaagg cgatcatcaa aggacttcct tcggtagaaa
     6241 cattaggttc tatgacagtg atctgttcag ataagacagg aacattgaca aaaaatgaaa
     6301 tgacagttgt ggatgtagct gcaaaagaag aagcctgcgt gttgtccatt atgaaaaact
     6361 gtcaggaatt aaaaacaaaa gaagaacaga aaacagaaaa tttaagtggc aatccgacag
     6421 aagttgcgct gattcgacac acagaaacag ccaatctacc tttgaaaaaa atagaagcaa
     6481 agattccgtt cagttctgaa tacaaatata tggcaacgat gcatgcggag gaaaaaggag
     6541 cggtgattta cgtcaaaggt gcaccagaag tattatttgc taaatcaact ctttcttctg
     6601 cagaacaaga agcatggagt cagacagctg cagaatttgc gagaaaaggt cagcgtgttt
     6661 taggatttgc ttataaaaaa gtagattcga aacaggaact cacgcatgag actttaaccc
     6721 aattaacttt tgcagggatc gccggattga tcgatccgcc aaaagaaagt gcagtcaaag
     6781 ctgtaaagga atgccaacaa gcagggatct cagtgaaaat gattacaggg gatcacaaag
     6841 acacagcaaa agcgattgcg gaacaaattg gcttaaaaca tactgccaaa gttttagaag
     6901 ggatcgattt ggatcttatg tctgatgaag agttgattca gcaagtgcca atagtcgatg
     6961 tctttgctcg aacgacacca gaacataaac tgcgaattgt taaagctttg cagaaaaatg
     7021 gagaaatcgt cggaatgacc ggagacggcg taaatgatgc acctgctttg aaaagatccg
     7081 atgtaggaat tgctatggga atcaaaggta gtgaagtaag caaacaggca gctgatatgg
     7141 ttttaggaga tgataatttc catacgattg caaaagccgt aaaagagggc cggcggattt
     7201 tggataattt gcaaaaaacc atcaatttct tcttgccaac tgcattagct caaggattga
     7261 tcttgatttg ggcacttatg ctgaatcgcc cgctaccttt gtccccagta cagattttat
     7321 gggtcaatat ggtaacgaca atcactttat cttatgcact agggttcgag tcgccagatc
     7381 cagaaatcat gaagcgccca ccaagagatc caaagcaagg aatcctatct ggttatcatt
     7441 tgtttcggat catctatgtc tccctactga tcatggttcc tgcctattta ttggctatgc
     7501 agtttgaagg gcaaagctta cagcaaacca ttcttctgca aaatatcgtc ttagctcaag
     7561 ctgtttacat gatcaattgc agagagttgt ccaagccgtc attgaaccaa ggtcttttgc
     7621 aaaacaaagc gctttttctt tcattaggta tccttgcttt attgcaaggg atggttattt
     7681 ttctaccagt cggccagcag ctgatcggta cagtaagttt aacgatgaca caacagttat
     7741 tgattggagc aaatgtgatt cttctattct tagttgtaga aatagaaaaa gggatcatga
     7801 acaaactgtt tcgcaaaaaa gaaaaaacag ctagagaata cagtcgctag ccgttactat
     7861 aaaaaagttt tgaaagtgag ttcagtcaaa cgattgagct catttttcgt tggaatagaa
     7921 gaaatgtata caatagggat atctagagaa tctagaggga aattggaaat caagatatct
     7981 gttttttcaa aagaaatagt ggaaagatcg gttagttcga taggtaagaa gcagacacgt
     8041 tttcctaagt aatcttttaa ttcctgctgc aagaacgcct tccatgaagg ttctccttgg
     8101 aaaataaaat agacattgaa caaattcggt ttgatagaaa aaatcgtttg ttggatcagt
     8161 agagacaaga gttggagaaa gtaatttggt tcattgattt ctatttcttt ttttagatcg
     8221 ttcagtaaat tctctttctg ttgatacaag tgtgcatatt gctgatcata tgtttctaaa
     8281 aaacgatggt attgacggtc caatgcttca atcaatatcg gcgattcatg atattttaat
     8341 atgaggagag ccaagttatc agtcagagat gtttttttta gttgatcaag agacagctct
     8401 gaagctagtt cctctacaaa cgtttcgagg acctctaaaa atggagaatg atgcaggcag
     8461 cgagcggttt cttgtccata gttattgttg taattccaac tttccagaaa gcagaaagaa
     8521 gcaaaagaaa gctcagtgtc tttaagataa ataccttccg ttaaatacaa ttttttcaat
     8581 gcagagtcat acagggagga gagtgtgctg ttggtatgga tagaagaatc taatcggcaa
     8641 ttcgctttta tacggatcgt attgataaag aaccaacatg ttccgaaaat ctcttctgtt
     8701 tcaaccgcta gttcagtttg actgaggttt cttaaaaatt gaaaataatg ggattcatgg
     8761 atcgaataat cctcgaaaaa atagttgtta tgagtgaaag gaatcagcaa tcgatgataa
     8821 aaagtacgaa tcgcacgctc ttttccaaga atacaagctg tatatttcgt caaatcgatc
     8881 tgcaagtcgt aacttgctaa acgctgattg attttagtca catgtctttt caatgtccca
     8941 taagaaagcc catggtcggc tagaaaatca gggacagcaa ctttttttgt aaaaagcaga
     9001 tttttcagca gttcgacact gactgattgt ttgaaaaaaa tctcccaaag gtcatttgtt
     9061 aataaatttt gctggttttg caaactgatt cctgcttgat cgctgatgat gtcccagcct
     9121 tcgggcagct gagagcgaat agattgaagg tcagaaaaaa tcgttcgttc ggttgtccct
     9181 atgatttcag ctaattcatt tacggttatc tttgtatgat tcagcaattg ttctactaaa
     9241 gaaacatgac gtcgaatatc tttttcggta atgatttttt tcattagtga atacatcaat
     9301 cagactccct tctattctaa gaatacggag aaaataaacg gtggtcaagt ttcaactgat
     9361 ttttcaatca ttgtttattt ttctggagca tttactgatg ataagaattt catcatgaac
     9421 ttgaaaaatg ataccactaa aatcaaaaaa taggtgggca aacaataata aacattttaa
     9481 tagtagatgg tagcaagacc aacacaggcg gattgaacat ttaagagttg ataaaaaaga
     9541 aaaatatcga agtatttgat tagcaactac ttgtattcat ttttctattc ttacgtggta
     9601 tctttgaata aagcgaatca ataagaaaga gatggaagca gatgctaacg aaaaaagaac
     9661 taatcgatca catcaatgaa acatacaatt tgtctccaga atatattttt aaaaaatttc
     9721 ctgattattg cgtattcaga catcaaaaaa gcaaaaaatg gtttggggtc ctgatgtctg
     9781 ttccagaaga aaaaatctat gatgatgcaa gtgaccatag gatcatcaat attcttgatt
     9841 tgaaggtcga tcctgaactt ggcggaattt tgcgaggaaa agaggggtat tttccagcct
     9901 atcacatgaa taaagaacat tggatctcta ttgatctacg taaaatagaa aagctagatg
     9961 aactagcaga tttgattgat ggaagtttta aatcaacgca atgaatgtct aggacaatag
    10021 agaagaggtt gtgacagaaa tgtttagctt caagaaataa gaaggaattt acgaaaattg
    10081 cttctcaaat ttttgggaaa tttcggctta ttttctaaga aattacttct ggaacaccgt
    10141 ttgttcgaaa aaagagctgc gacaagaatt ttgtcccagc tctttttttt ttattttcct
    10201 aaataaaatt cccgcatcgt ttttgcaatc attttgatca aaggatgatc ttctgtgaag
    10261 ctagaagata aataaattgt tcgtgtcaag ggcaaatctt tgatttcccg caagacgaaa
    10321 ggatcatttt tatcgtaaga ccaagaaact tttgggaaaa agctaacagc taatccttgc
    10381 ttgacaatag aacgtaatgt cgcaggatca tctgtcgtcg aactgatcgt caattcgata
    10441 cttttcgctt caagtgctgc atccagtgta tgacggaagg cgttgctctt tgacagcatc
    10501 aagatggggg tgcgttggat atcttctaat tgcagatgtt cttttttagc aagaggatgt
    10561 ttcttaggaa tggcgacata aacaggttct ttcatgatag gaatattggt catgccaggc
    10621 ttcatctccg aagtgatgta cagtacagga tcggtaatat catctggtaa atgatgttgc
    10681 atgatcgtta attggatatc aggatgcttt tccataaaaa ttcggaccaa gttcggaata
    10741 gaaacagagg ctacctctaa atacaatatg acggaagtaa cgatcttctc attgaccgaa
    10801 gacaataatt tgattccttc ttctaatgtg tctaatgtct tgactgcata tggataaaat
    10861 tcttctgcat aaacggtcgt ctcaatgttt ctgccgctat ggtaaaacaa agggacacct
    10921 aattcgtttt ccaaatgttt gatcgcacgg ctcaaggcag gttgagaaat aaataattct
    10981 ttggctgctt gggtaacatt ttttgtttcg aatacttttt taaaatactg cagatgaagc
    11041 aatttcataa ttatccatcc catccatacc tttttgttat ggttttttct ttttttggca
    11101 ttttttatta tagcagaaat tgactaagat agcagtggaa aaagtaagtg ctgaggaggc
    11161 gaggctcatg agtacaggtt tcttatttcc tggacaaggc agtcagaagt tagggatgtt
    11221 agcggatatc cctgaaatgt acatggaacg tttagaaaat gtgacggggt atcggttagt
    11281 tgataaagaa gaaaactatc aagatacggt ttttatccag ctagcattac tgacaaaagc
    11341 agtattttat ttagatgaaa tggaaaaaaa cggtattcag cctgatattg cagcaggaca
    11401 ttcgattggt gctttttgtg cagctgttgc ttgtcgatct ttgagatttg agaatgcagc
    11461 ggaactagtc tatcaccgcg cagtcttaat gaaagaagct tatccaagcg gttatgcaat
    11521 gggcgtagta gtaggtgtga cgagaagcga agctgaggaa attgtagcaa aaacatttga
    11581 tcctgaccat ccagtttatc tgtcaaatga aaattgtccg atgcaacata cgttatctgg
    11641 gagtatcgaa ggtttatccg ttacgttggc agaagccaaa cggcaccaag cgcaaacagc
    11701 gaagctgctg aaagtccctg tgccatctca ctgtctcttg atgaatgata cggtcaagaa
    11761 attcaagccg tatatgcagt ctgtccatct atctgatgct agatgtttgt atataaaaaa
    11821 tacggatgga agagccactc aggacgcaga agagattcgt aaagatttgt tggaaaacct
    11881 agctttccct gtgcagtgga accaaatgat ggacttggca aaagaattag gaatggatct
    11941 gacgattgaa tttccccccg gcaatacttt gacaaagctg attcatacaa aatttggtgt
    12001 agataaaggg atccgtacga tcaatctaga ccaacatggt attgatgacg ccacgtttct
    12061 atatcaaaaa tggagatgac taattatgga aaaaccaaac cgcaactggg cttctaaacg
    12121 cgaaaaaaaa gcgcagcgat tggaaaaaat cagcaacttg ctcaatggca agtatgtaga
    12181 aactgaaaaa atcgtagaag taatggaaaa actgattgct cccggcgaca aagttgtatt
    12241 agaaggcgac aatcaaaaac aagcaagttt tctttcagaa agtcttgaaa aagtagatgc
    12301 agaaaaagtg aatgatctgc atttgatcat gtctagtatc tcacgaccag aacatttgaa
    12361 tatttttgaa aaaggaatcg caagcaagat cgatttctct tatgctggtg cacaaagctt
    12421 gcgtgtcgcc caaatgattg aagacggcac aatgaaatta ggtgatatcc atacttattt
    12481 ggaactttat ggacgcttat tcatcgattt gattccaaat gttgttttag tagctgcaga
    12541 taaagcagat aaagacggca acttgtacac aggtttcaat acggaagaaa caccaacaat
    12601 catcgaagct gcagctttca aagatggtat cgtgatcgtt caagttaacg aagttgtcga
    12661 ttctttacca cgtgtagata tccctggtga ctgggtagac gttgtcgtga aagcagataa
    12721 accttatcaa ttagaagctt tattcacacg agatcctcaa aacatcacag aattgcaaat
    12781 tctaatggca atgatggcaa tcaaaggaat ctacgcagaa cacggcgtac aatctttgaa
    12841 tcacggtatt ggattcaata cagcagctat cgaattgtta ctgccaactt atggtgaatc
    12901 actagggtta aaagggaaaa tcgcgaagaa ctgggtattg aacccacatc caacgatgat
    12961 tccagccatc gaatcaggtt gggttgaaag catccatagt tttggtggcg aagttggtat
    13021 ggaaaaatat attgctgctc gcccagatgt cttttttgtc ggtaaagacg gcacgatgcg
    13081 cagtaaccga acattatcac aagctgcagg acaatatgcc atcgatatgt ttatcggttc
    13141 aaccttgcaa ttagattacg aaggaaactc ttctaccgta acaaaaggac gtttatctgg
    13201 ttttggcggt gcgcctaaca tgggacataa tcctggcggt cgtcgtcatt ctacaccagc
    13261 atggcaatca cttcgcgaca atgatgatcc attagggaaa ggccaaaaat tagttgttca
    13321 aatggttgaa acatatggtt caaataaaaa accagtcttt gtcgaagaat tagatgcact
    13381 agctgttcaa aaacaagcag gcttagccaa tgcaatgcac cagtgatgat ttatagcgaa
    13441 gatactacac atatcgtgac agaagaagga attgcttatt tgtacaaagc aaaaacaaaa
    13501 gaagaacgac aagcagcaat tgcagcaatt ggcggtgtta cacctcttgg aatgactagc
    13561 acgaaagaat cactagatag cttgcgtgca gccggcatcg tgaaattacc agaagatcta
    13621 ggcatcaaac gtagtgaagc gaaacgttca ttattagctg cacaaaccat cgatgatttg
    13681 atcgaatggt cagatggcct gtatgaacca ccaatgaaat tcagaacttg gtcataaaat
    13741 tggcgagaaa aaccttttgt cgccatttac ccagatggaa gaagaaagac gaataagcag
    13801 aacgtgtatg ctctgtttta caaaaagggg agagatccat ggaacaatta gcttataaat
    13861 atccaacgaa acagccgatc aataagaaaa tccatgtagg agtggtcggt tcaggagatt
    13921 tggaaatctt gatgtttcca acagaaaaaa cagaatctac tgtaaaaatt tgtacaggaa
    13981 gcgatggctt cggcgaagta tggaacaatg tattaacgag attttttgat cgttatccaa
    14041 tttcagcaga tatcgtgatc aatgacttcg gtgctacacc aggagttgtg tacctacgat
    14101 tgacgcaagc aatggaggaa ctttcagatg aaaaatagtt ttgtagaatt aaacgccaga
    14161 gaacgagtga acgctctact tgacaatgga cgtgaattga ttgatccatt cgatcaaatc
    14221 atcgcgccaa gtttaccagc acaaggaatc gtccctgaaa gtgatgacgg gatcgttgtc
    14281 agccgtggca cgatcaatgg caaacaagca gtagtaatcg ctatggaagg aaaattccaa
    14341 ggtggcggta tcggtgaagt ttccggtgca aaaatcgttg ctgcacttga acatgtgtta
    14401 gaagaaaata aaaaaggaaa tgagatcttt ccagtcattg tactagatac aggtggtgtt
    14461 cgtttgcaag aagcaaacta cggactgtta tctatttcag aaatcggaaa tgtaatcatt
    14521 gctctaaaac aatatgtacc agtcatcggt ttgattccag gacgtgtagg atcatttggc
    14581 ggtatgtcga ttacttcagc attaatgagt tatttaatcg caacaaaaaa agcgagagtc
    14641 ggtttgaatg gaccagaagt tattgaacaa gaagcaggcg ttcgagaatt tgattctagt
    14701 gacaaagact tgatttggaa tacattagga tctcgtcaac gggtagaatc aaaaatcatt
    14761 gatgaattag tgactgatag tgtagaagca atcaaagctg ctgtcatcgc agcaatggac
    14821 gaacgaaaag acacgcatcg cagtgaaagc tcagcatttt atttatcatt acttgattct
    14881 ttagatcttt caaaaccaat ggccattgac gattacaatg aagcaattgc taaccatcaa
    14941 gcaaaatcaa ttgacttacc agaagtagca gaaggaaatg aaccagctgc agcaagtgtt
    15001 ggttacgatt ggtttgcggc actaactggc atgaaaaatc caacttcttc tattagtact
    15061 gtgtattatg gtaaatcaag taaattcata gaagaagcca tcgtgacatc aatcgtacct
    15121 gatcctaaaa acccgatgta ccgtgtacga aacggtgaag ttggtttagt tgaaggtttc
    15181 cgcatgggac aaattttgga tcacgtttac gaagaagata aagacaaaaa agtgaaacgg
    15241 ccaatcatcg ttgtgatcga tgtaccaagc caagcatacg gctataacga agaattgatc
    15301 ggtatccatg tggctttggc taatagtgct gctgcttatg caaaactccg tcaagctgga
    15361 catccagtta ttggattgat tgcaggaaat gctatttcag gtgcattttt agcacatgga
    15421 ttacaatcaa gccgcttgat tgctttaaac aataaagaaa ttaccgtcca agcgatgtct
    15481 aaagaaagtg ctgcacgtgt gacaaaacgt tcggttgctg aaatcgaagc agctgctgaa
    15541 aaagttccag ctatcgctta cgatatcaat aattatacaa aactaggagc gatctatcat
    15601 ttcctagaag atatcaccga ccaagctgct tcagaacaaa atgtcagcaa agtcatcgaa
    15661 gcaatcaatg aagccattca agatgtccgc aatacaaatg acacgatgct gacaaatcga
    15721 tacacaaacg aaacagccaa aacattcgga cgtgtcgaaa caaataaagt ccgtgccaaa
    15781 atggacgaag aatgggatgc ataaaaaatg attcaagcac atgatattgt cctttttgat
    15841 aaacatgaaa tagaagggtt caccttgcct gaatgggtcg ttctaactga aaagatgttc
    15901 ggaacagttc gtcgtgcgtc gacggatcac gcagaaatac taccgattgg tttacgtgga
    15961 cctgcccgca atcagagatg gtcaacagaa atctcaaaac atgccgtcaa acaagtcatt
    16021 catccgtggg acatcatcga acaagaatcg tatagacaag agaaaatcat ggattatcct
    16081 gtttatcagc aatttcatgc tgctagaaac atattatcag gatgtaaatg gggaatcggc
    16141 ggcagcttag gatttgaatt atcgactggg attccggcag taaaagaaac cagtgatttt
    16201 gatttgctgc tgtacgcaaa ttcaccgatt gaattgccaa ttcaggtaat tcaatcgcat
    16261 cctgcttttt ttgagcaatt cgatacccaa gtagtgactc ggcaaggcgg tttttcacta
    16321 aaagagtatt tgagagagcc agagaaaaaa attctattga aaacagtcga aggacctaaa
    16381 ttaaccaaag aactttggta ataaagaaaa agagagagaa agagatgttt gaaaacaaaa
    16441 aaatcaaaat caacgtaagt gacttccaga aagtatcagc tgtaacggct gttatgatgc
    16501 aaacgatatt gagctttgct ttggcaaaca gccacagcgc aacaaataca gcttttcttg
    16561 gtacctttta cgtcatcgtt aaatacacag caccaatgtt tatttttgca attgtttata
    16621 atatggtaaa aacaagtcag catttgtctt attgggaatt tttgaaagaa aagttttttg
    16681 aactagtcgt tccgtatctt ctatggacga cggcttatct ttggctattt ccagcagtcc
    16741 aacaacaaac tccgtatacg gatactgcaa gctttttact gaaatatgtc acaggtgatg
    16801 gagcgcctca tttatggtat accgttatga tgctgcagat ccaattgttc atgccgtttt
    16861 ttgtttggct cggttacaaa gtatttgcta ataaaaaata tgtatggcca gtattgattg
    16921 gttcaacgat tttatatgtc gcatggtatc tgttctatca aacacaagta ttgactgggc
    16981 cttatcatga cagctggtac ttattagatc ggtttgttgc tagtttcatg atttatgggg
    17041 tagccgcatt tgtttatcac gaaaaagtct atcaatattt ggacagagta cgttatcttt
    17101 tcttacctgt tgccttagtt atcgcgttct tttctgtccg ttcattgctc gcacatccag
    17161 gagacttgag ctttgcgaat gcaccttact tgaatacgat ccaaagttta tacagtttag
    17221 tcatcatctt tgctgtcttc ataggcgctt ccaaaatgat tgtaaacgat tcgccaaaat
    17281 tgccattgtt caaatggttg tctgtttatg cttatcgtac gtatttagcc aatgtatttg
    17341 ttttccaagt actgttatta ttgtttaaag atagttggct gcagttacct tcaggagtca
    17401 tgattcttgt agcttatcta atgacggcaa gttgtgcatt tgctttaagc tggttacttc
    17461 atatcatttg ggtagctatc aaaaaaggtt tcagcaagta atagtaggaa gaaggagtga
    17521 gtgtcatgat aaatgacgaa cgaaaaaaag cagtgactca gcaactcgct gcgttggtag
    17581 aggaagcatt gatcgcagaa gtaacattaa gtccaaaacc tggattagtg gatgcactgg
    17641 atgcgggtgc acattccgat atggactatg ctttatttct taagtctgct aagacgctaa
    17701 ctcctttttt tgaagaaatg gctcaaatag catggcatca tgctattgat caggagttaa
    17761 gagaaagaat tgctgagatt gggcgaaaag cggaaaaggc aatgctgatt gctacaaatg
    17821 gcatcaatac ccataaagga gcgatttggg caatggggct attgatcagt gtcattgcac
    17881 aaaaaatcag tatggaacag cctttttctt tatcgaaaat acttgatgat gtcagcaagc
    17941 ttgctgcttt tcctgataca aaatatcgtc ataaaaaaac cactcatggt catagggtga
    18001 agcaaaaata tggcgtaaat ggtgcatatg aagaagcgat attagggtat ccgcatatca
    18061 ggcttgcaat tcaagcagct aaggagctag ataatgattc atatgtacag aaacaaatgc
    18121 atatgttgtt agttttgatg agttcgctag atgacacatg cgtattgcat cgcagtgatc
    18181 agaaaacgtt agtggaaatg caaaaacttg ctcatgctgc tagtcaaagt gctttaccaa
    18241 atcgtccatt tacagcactg ttgcactttt gtcaggaaaa ccaaatctct cctggaggaa
    18301 gcgcagatct cttgtctgca agcttgttca tgctgaagat agaggaatta tggactgccg
    18361 acacatacat ttgtccgatg ttactcaatg actaaataag acaaaattgc taaaaaaaca
    18421 gtttatgcat ctggttattg actcttcctt ttagcaaggc tatcataaaa atagtttttt
    18481 gtaataaaag ataacgagga agacaatgaa aaaaatcaat tatttagcaa tcattttgtt
    18541 agtgggccta tgtttgtttg ctctgccgac ccaaggattc gctgtcgcgg gtggtcatgg
    18601 tggaggtaca tctagcggtc gaagttcggg cagaggttca tttggtggtc gttcatatgg
    18661 gggcagttat tttggcagtc gttcttacgg cagagattct tatgggtatt atggtccagg
    18721 tacaggtcct tctagttatg agcaaacggg atttcttgca ttagctgttt tgtcagtagg
    18781 aggggtttct gctttttcta tcgtcaagaa taaaagacaa aagaaaaggc tgaaagcgga
    18841 gatattgtcc cgcatgtcag gaacggctaa agaaaagaaa gcacagtttc tggaaatcca
    18901 aaagatattc atgaagatcc aatccgcttg ggaagaagaa actcctgaaa aagccagaag
    18961 ctgttataca gacaagctgt ttgccgaaca tcagaaagtt ctgcaaaaaa acaaagcaga
    19021 cggtgtaaga aatcatacta aaaaaataac tattcaggaa gtaggaaatt atcgacagat
    19081 ccacgaaaat agttttgcca tccgaattga tttcagatgc tgtgattatt cagtcgatcg
    19141 aaaagatggg cgattgatct caggaagtaa gacacaacga cagtattttt ctcagatatg
    19201 gtattttgat ttttctgaga aagaaaaaag atggcaagcc gattttattc agccgattat
    19261 gctggattga caggaaatat ttatgaacgg agttgtgaca aaagccttgt cgcagctcct
    19321 ttttctgaat aaacggtgtg ccagagatag cctatcggca ataaggcacg attttgtaaa
    19381 atatgggaag cgatttttca aaatcgcttc ttatcgctca aggctgaaca tctctgtcac
    19441 aacctctcca ctaaatggtg tttaaaagct tgcatctgcg ataataagaa caaccaaacg
    19501 aaaaatagga agcactattt tagtttagtt gttcttatta cttgatgcag gacagctttt
    19561 tcacaacctc ttttttgtat agatgggcaa aatttttgta ataataggcg tatgtctttt
    19621 tcgttgagga gatgaaaaag catatggctt attttttgtt tgctttgctt actttctttc
    19681 cacttcaagt ggcagaaata tctacttttt attatgtaag cggaaccata tacttgaaat
    19741 cagaaggaga tcatttctat gaaaacaaga acaattgaaa tacttcacag aatcatgaca
    19801 gcccaatatc cagtaaaagc aaaacatcta gcaagtgagt tcaatgtcag tcagcggacg
    19861 atccgtcagg aagttcttga agcgaatatc tggttgagca gtaaaaagct tccggagatc
    19921 cgtacaattc gcaacaaagg ttttttgttg aagctggaaa aggaaaagca agctcgatta
    19981 gaaactgaaa taaaagatgt gaaagacggc tttttgaatc ggcgcgaacg tatttttgat
    20041 ctagttttag ccgttgctta cagccgaatc ccgatccatc taaacaaaaa agaagaagaa
    20101 tttcaaatct ctaaaagtac attagatgaa gatatgagac ggttacgagc aacactggtc
    20161 agttatggga tagagatcgt cagttatgga aagcaaggac tggtttataa aggttcagaa
    20221 cgatctatcc gtacgatgat ctatgatttt atcaatcaga atctgggaat gaccgacttc
    20281 tttatgaatc aagatgcaca attaacaccg gctcaaaaaa tctttctcca ttattttcct
    20341 ttggtggaaa tcaaaaaaat acaggagatc tattccaata agttgttgaa acaagaagat
    20401 gatatttata aaaaacaaat cctgcttttt acattgatct ggatccagcg aatcaggaaa
    20461 catgaattta tctctgccat taattggaaa aatacagaaa taaaagacag tagcttttct
    20521 gcatttattg accacatgat cgaggaattt caattggaca atgtcccgca agtagaaaga
    20581 aattatcttc gtttcactat tgagacgttc aatgctagag atatctccaa ttcaatcgag
    20641 tgggtacaag ctcaattact aacgatccaa ttgatccaat ttgtagagaa cgagacacac
    20701 gtacctttcc atctaaagga agaagcttta ttcgaaaatc tctataagca catggctgca
    20761 ctgattgtaa gaatcaagaa tcatatccaa gtcatgaatc cattgaaaga aaacatccga
    20821 acaaattatt tcccaatcta ccgcgcagtc actcattttg ttcctacgat cgaagaagta
    20881 attggtggca agatactgga ggacgagata gcttttcttg tcatccactt ttcgacgatc
    20941 gccagtgtaa tcaagcgaga cttgaattat gtttataaat cagtcgttgt ctgtaaccat
    21001 gggatggcta ctggaaatct gttagcagag aatctgaaag aaaagtttcc tcagatcgag
    21061 attactgcag ttttaagttc aaaagaaatc gaattagtag ataaattgga tgtcaatctg
    21121 gttttcagca cctttcaatt gaattatcga aataagccgc ttttagttgt cgatccgatt
    21181 ttaactgaga cgaatatctt ggttatagaa gactttttga aaagaaatga agaactacag
    21241 cggttagagc ctaacagtca tgaatcgacg gagttattca ctcaaataat agatgtcatg
    21301 aaagaaagtg gtggccgagt agatcgaatc atctatggca agttagaaaa attattcgaa
    21361 acaaacaatt tagaaatcaa caaaagggag atccagccta tgttaaaaga tattttgacg
    21421 gacagcaata tcatgatcaa agaaaaagcc actacttggg aggaatcgat tgaactagct
    21481 gctagaccat tggttaaaga aaacattgtc gaagagcgct atgtcgaagc gatgattcgt
    21541 gcagtgaatg attacggacc gtatatcgtg attggcaaac acatggcact agcccatgcc
    21601 cgaccagaag atggggtcaa taaattagga gtcagcgtag ctacgattga gcaaccaatc
    21661 gatttcggta atcctgaaat ggatccagta aagattattt tttgtttggc agcagtagac
    21721 tcctactcgc atctgaacat catgaaagaa ctaattgaat tgatcaacaa cgaagagaag
    21781 ttggatcgat tgattgaatg cacaaatatc gaagatttca aacaactgct tttttcagta
    21841 gttgagaaat aataaatgat acgggggaga acaaagatga acaaattgaa cattttattc
    21901 gtatgcggtg caggtttagg aagtagtttt gcagcacaga tgtctgctga ggatgttttg
    21961 aacaaattag gagtagaagc caaactggac cattgtgata tttcttccgc agtttccatg
    22021 aaaccagatg tgatcatcac agcagaaaat tttcgttcgc agttcgaaaa gttcgcagtt
    22081 gatcctaaaa cggcgatgat ctatttgaaa aatattgttt caaaaaatga aattgaagaa
    22141 aaaatcaccc cagttctgca agataaggga gttatctaaa tacaaaaaga aaagagggta
    22201 aaaa
//
LOCUS       DADVXU010000046        21386 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.048, whole genome shotgun sequence.
ACCESSION   DADVXU010000046 DADVXU010000000
VERSION     DADVXU010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 21386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.048"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            67..768
                     /locus_tag="KB953_001574"
     CDS             67..768
                     /locus_tag="KB953_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBB6866552.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1377..2393)
                     /gene="tsaD"
                     /locus_tag="KB953_001575"
     CDS             complement(1377..2393)
                     /gene="tsaD"
                     /locus_tag="KB953_001575"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBB6866553.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2390..2854)
                     /gene="rimI"
                     /locus_tag="KB953_001576"
     CDS             complement(2390..2854)
                     /gene="rimI"
                     /locus_tag="KB953_001576"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBB6866554.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2861..3403)
                     /gene="rimI"
                     /locus_tag="KB953_001577"
     CDS             complement(2861..3403)
                     /gene="rimI"
                     /locus_tag="KB953_001577"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBB6866555.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3387..4109)
                     /gene="tsaB"
                     /locus_tag="KB953_001578"
     CDS             complement(3387..4109)
                     /gene="tsaB"
                     /locus_tag="KB953_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBB6866556.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4300..5280)
                     /locus_tag="KB953_001579"
     CDS             complement(4300..5280)
                     /locus_tag="KB953_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBB6866557.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5304..6788)
                     /gene="galT"
                     /locus_tag="KB953_001580"
     CDS             complement(5304..6788)
                     /gene="galT"
                     /locus_tag="KB953_001580"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HBB6866558.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6800..7789)
                     /gene="galE"
                     /locus_tag="KB953_001581"
     CDS             complement(6800..7789)
                     /gene="galE"
                     /locus_tag="KB953_001581"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBB6866559.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            8037..8204
                     /locus_tag="KB953_001582"
     CDS             8037..8204
                     /locus_tag="KB953_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HBB6866560.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(8266..10077)
                     /locus_tag="KB953_001583"
     CDS             complement(8266..10077)
                     /locus_tag="KB953_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HBB6866561.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(10074..10439)
                     /locus_tag="KB953_001584"
     CDS             complement(10074..10439)
                     /locus_tag="KB953_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HBB6866562.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(10602..10997)
                     /locus_tag="KB953_001585"
     CDS             complement(10602..10997)
                     /locus_tag="KB953_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBB6866563.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(11015..11977)
                     /locus_tag="KB953_001586"
     CDS             complement(11015..11977)
                     /locus_tag="KB953_001586"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HBB6866564.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(11977..12189)
                     /locus_tag="KB953_001587"
     CDS             complement(11977..12189)
                     /locus_tag="KB953_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HBB6866565.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(12210..12908)
                     /locus_tag="KB953_001588"
     CDS             complement(12210..12908)
                     /locus_tag="KB953_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HBB6866566.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(12928..13470)
                     /locus_tag="KB953_001589"
     CDS             complement(12928..13470)
                     /locus_tag="KB953_001589"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBB6866567.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(13602..14606)
                     /locus_tag="KB953_001590"
     CDS             complement(13602..14606)
                     /locus_tag="KB953_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBB6866568.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(14603..15592)
                     /locus_tag="KB953_001591"
     CDS             complement(14603..15592)
                     /locus_tag="KB953_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBB6866569.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(15589..16395)
                     /locus_tag="KB953_001592"
     CDS             complement(15589..16395)
                     /locus_tag="KB953_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6866570.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            16561..17517
                     /locus_tag="KB953_001593"
     CDS             16561..17517
                     /locus_tag="KB953_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBB6866571.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(17594..18112)
                     /locus_tag="KB953_001594"
     CDS             complement(17594..18112)
                     /locus_tag="KB953_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBB6866572.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(18200..18349)
                     /gene="rpmG"
                     /locus_tag="KB953_001595"
     CDS             complement(18200..18349)
                     /gene="rpmG"
                     /locus_tag="KB953_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBB6866573.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            18577..19023
                     /locus_tag="KB953_001596"
     CDS             18577..19023
                     /locus_tag="KB953_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HBB6866574.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(19218..21113)
                     /gene="asnB"
                     /locus_tag="KB953_001597"
     CDS             complement(19218..21113)
                     /gene="asnB"
                     /locus_tag="KB953_001597"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBB6866575.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         6431 a         4546 c         3692 g         6717 t
ORIGIN      
        1 aaactttaca aattctttaa aaaaagtgac aataaatata gtgaacaata aggagaggac
       61 tttttaatgc gtgttaataa aatcgatttt cacttttcaa aactcagata tcaacctgat
      121 tttgaacatt tctcggatga agagttccaa cagatcaagg aacatactgt tcttcgttct
      181 tataaaaaag gacagatcct tttcgacgat ggtgatccca gacaacggtt gtatatcttg
      241 accaaaggac tcgttcgttt agaacgttat gatgaaagtg caacttatta ctactatgac
      301 tatgtgaata caaataacct ttttcctatg tcaggaatgt ttggagaaga gacctacact
      361 tatacagctc aagcgatgac tgatattgaa acattctacc ttcctgtttc ctactatgaa
      421 aaattagcac gaaaaaatcc cgaacagttg atctatatga tacaaaaaat ttctaaagta
      481 gttgaaatac atgaattgcg tatccaaatc ggattgacct ctagcgcctt tgaccgggta
      541 aaacaaaatt tattcatttt gaaagaagaa ttgggcgtac caacaaaaga tggtcatatc
      601 accattcctt atccgatcac cttaaaagaa ttagcagtga atagcggcac gacacgtgaa
      661 actgctggac aagtaatcaa acaactacgt gattccgggc tgttagatta tgacaaaaag
      721 cgttttactt tctgtaagac agctactact ccagctatga gagaataacg cgaacaaaaa
      781 taagcagtga ccctgataaa tatcagggtc actgcttatt ttcctcccta aaacttctga
      841 aagatccggc agttttagat cactggacaa gactcaaaga ttttctatgt gtgtttcagt
      901 tatgtaatat tcactgtttg caaaaaatac tgttagatta actagatgaa gcgtcttgtc
      961 ctcatccgat ctagattatt cgatattcct cgacaataaa cgattactca gtgagggcaa
     1021 tcagaggaac ggataatgat aaataagctg taaaaaatag caaaataaca ataatgatta
     1081 aaagtatgta aaaaataggg ctttgacaaa aacgtgtcac agccctattt cgattaaacg
     1141 gtgtgtacct gtcaactctt cggtcacaag tcataatgtt cataaacatg aggagctgtt
     1201 ttttgaaatt gttccttgtg aatcagagtt aaacgacagg cttacgaact cttgattaaa
     1261 cggcgttcaa aagcttgcat acgcggtaat aagaacagcc aggtcaaaaa catgagaagc
     1321 cgtttttcct gggctgttct tattacttga tgcagggcag ctttttcaca acctctttat
     1381 tcagataaaa ccaagcttgg atcagcattc aactgatagc tagcaaaatt tcttttttgc
     1441 atttccacat gtgctgctgc tccaatcatt gcagcattat ctccacaaag tcgaagaggg
     1501 ggaatcacta gttctacctc agggagttta gctgccaaag catcctgcaa tccttcacgc
     1561 aacccttgat tggccgctac acctccagct ataatcagtt gcttgactgg ataattttga
     1621 caagctcgaa gtgttttagt aatcaataca tcgatcacac ttgcttgaaa gctagctgct
     1681 aaatcatttt tgtctagatc ttctccacgc tgttgtgcat tatgcaccaa attgataaat
     1741 gcacttttta atccgctaaa actgaaatca taattatctt catggatcat cgctcgcgga
     1801 aaatggtaat tatcttttcc ctgatgagct agctgatcga tttcttttcc gcttgggtaa
     1861 gaaagaccta atactcttcc aactttgtca taagcttctc ctgctgcgtc atctcttgtc
     1921 tccccaatga tttcatatga tccatcttct tgcatataga ctaattctgt atgtcctccg
     1981 ctaacaagaa gtgccatcaa tggaaactgg aatggtttaa ccagacgggc tgcatagata
     2041 tgccctgcca tatggtttac aggaataagc ggcaattggt gtgcccaagc aaaagcctta
     2101 gctgcagaaa taccaatcag caacgagccg acaagtcctg gtccataagt gacagcgact
     2161 gccgataaat cttctgccga cacaccagct tctaccagtg catcttccaa gcataaggtg
     2221 atttgttcta cgtgatggcg gctagctact tcaggtacta cgccaccaaa acgtttatga
     2281 ctgtttattt gagaagcaac gatgttagat aagatctctg ttccatttct aacgaccgct
     2341 acactcgttt catcacaact tgattcaatc gccagaatca attcttcatt catttcatcg
     2401 tctccgtttt taactttctg ctcataatga tcgcatcttc cgtcggattg cgataatatg
     2461 atttccgttt acctaatata cgaaactttt ctgattcata aagttttctt gctgcttcat
     2521 tagatagacg tacttctaaa aaaacatgca gcgaatcttt tatttgttcc gtaatcagta
     2581 attgtaacaa atgccgtgca tgcccttttc cttgttctga aactgcaaca gcaatatttg
     2641 tgatctcgac ttcatctatt actttcgagt atccaataaa gccacagatc tggttatctt
     2701 cagctaaaag cagataatca gagtgaggct gttggatgtc tgccaaaaac tgcattttcg
     2761 tccaaggtga accatgtgga taagcatttt cgctaatcat ccatacgcat tctgccaagt
     2821 acgcttcatc aaaatctttt tttctgcaaa tcaactaatc tcactcattt tctaaaagtt
     2881 ttatcatatc caacgcatct tcttttgttt cagtgtaata tctttttttg atcgttgttg
     2941 cttgaaaccc caatttccga taaagtcttt gtgcatcttg attactcata cgcacttcta
     3001 atgacatcgt ctcacagcga ttcattatag caaagttctc gatttcgtca ataagcagcg
     3061 aaccgattcg tttacgctga tatgccggtc gaacggcaat attcgtgata tggcaatcta
     3121 aaccgaacgc tcttgaaccg ataaaaccaa tcattcttcc attatcaaag atacctagat
     3181 atagatgagg aagcggtgac ttcaattctg atataaaggc actttttgtc catggcaatt
     3241 cgccattata gacttctcgt tcgacatcca gcagctcttt gatatcttct gtagtcaatt
     3301 ctcttagtac gtaacgacga tcgatttgtt cgactgtttt ttctgtataa atcattttat
     3361 cttgaaaaaa gcttctggca tacgctctaa attttttcaa cataactttc gtctccaggt
     3421 gtgtgcgtct ccaaccattt ttcttctgct tctactcgtt ttaaatagcg aggaagaaaa
     3481 gcttgtttct ctgcaactgg ttcaactaaa gcacctaatt gagccacagt tacaccactt
     3541 ggaatatccc atgctggaac tgtattaatc atcgcactag gcaatatttc ctttatttca
     3601 gagaagaatt tgtgacaatc actcccgacg aaataaatag cttcattcag cttttgtagt
     3661 tgtgtgaaca aatcagaagc agaaatatgt acatcttcaa gtacagtttc taatctattt
     3721 tctttccagc ggtatgcccc agcgtataca ttttttcttc gagcatcaaa catgggcaca
     3781 ataatattat ccacaccgat gcaattagcc gcgatggttt tcagactaga tactcctact
     3841 agctctttat ctaatgtata agcaatcgtt tttgctgtcg ttacacctat acgaagccct
     3901 gtataagaac cgggaccatc agaaaccgcg attcggtcaa tgtcttttgg acttatttga
     3961 agatctgcaa acaattggtc gattgcaggc atcagtgtta cactgtggtt gcgtttgata
     4021 tttgtttgta tttgtccaag aatttgttcg tcttctacca caccgatcgc caaagtttgg
     4081 tttgcagtat ctattcctaa tgtaatcata tatttgatcc tttctgctat gacattctcg
     4141 ccttccattg tagcatactt ttttattctg aggatgaaaa taccaaaggc aaaactagta
     4201 caataataca tgattatctg ctggcgggca aattttgccc cttcctgaaa cacaaaaaag
     4261 ctgcggtatc actccatttt cccacagctt ttccatcttt tatttcgttg attctcgaat
     4321 aatcaactct gtttccacaa taatctttct tgaaaccggt gctttggaat tacttaattg
     4381 aagaatcgtc tccactgcta gctctcccat ccattcggta taaactttta cagtagaaag
     4441 cgagggcgtt acatatttcg ctacactgac atcattgaag cccatcacag aaatatcttc
     4501 tggtaccact aaaccttttt cttgaatcgc tttcagtgcg ccaatcgcta atgcatcatt
     4561 agctgcaaaa agcgcatcag gaacatgcgg attcgttttt aaatattgct tcactgcttg
     4621 atatcctgac tcgacagaaa aatctgcttc gataattgga ccttcgtcgt agcctttttc
     4681 tgccaaaact tgttgaaatg ccataattct gggatctttt agccgctgat ggtttttctt
     4741 cgtatattct gttccagcaa gcatagcgat atgttgcgct ttatgggaga caaagtgttc
     4801 aatgactttt tgaacacttg acttaaagtc gacaactaga gagtcgattc cttgagaagt
     4861 gccgtcagaa tctaccaaaa gcaaggcatt ttgggatcct ttcatttcta ataactgagc
     4921 ttcgtcgaat tttcctagtg cgatcgttcc atctgccgga agatcactaa tggaatccca
     4981 cgtttctttc aataaagtaa cgtttgcttc ttctgccttt ttttcgattc ctaggcgaat
     5041 agataaataa taaagatctt caagctcttc cgtatcgtcg taccactgga tcaggcgaag
     5101 cgtttgctta gtatttgctg catttttacg atgtttcgta taatttaagc gttctgctgc
     5161 ttcaaaaatt ttttgctttg tggctaagcc aacggaaaga tcaggatcat agttgagaac
     5221 tcttgaaaca gtagcaggag aaactcctgc caactgcgca atgtctttaa ttgttgccat
     5281 ttgtctctcc tcctttttta cgtttataaa atcgcagtaa atcgttcaaa cgctgcctgt
     5341 cctttttcat ctcgcttgaa tactccagcg tcttccagta cacgtgcaaa aacctgacct
     5401 acagattttt gtacgacatc ggtgaccgta tctttcgtca aatcaggatg ttcttttttt
     5461 aattgttctg cccacggacg atgatattca gccatctggt tttcttctcc taataagaaa
     5521 tgctctactt ctactagttc tggtttcaaa cgaggtggta aaacagcaag ccccattact
     5581 tcgatcaacc caatgttctc ctttttgata tgttggacat ctttatgtgg atgaaaaatc
     5641 ccatcaggat gttcttctga aacattgttg tctcgaagga caaggtccaa ctcaaagtaa
     5701 ttgtcccttc ttcgtgcaat tggcgtaatc gtatggtgtg gtgtaccatc tgctgaaaaa
     5761 gctctgatag atactgtttc atccgaatat gctttccatt tttccaatac tgagttagct
     5821 gcttcaataa gcaagttttt ctctgttgat tgcaaacgga tcacagacat cggccatttc
     5881 acgactcctg cgatgacttg cggataatct tctagtttga taagtttttc tatcggtgct
     5941 tttgccatag gaaattcatg acgtcctgcc tgataatgat catgagaaag aatcgatcct
     6001 ccaacaattg gtaagtcagc attcgaacca acaaagtaat gaggcaagac ttcagtaatc
     6061 cgaagcagtc tagcaaatgt ttcctttgtg attttcatcg ggcggtgttg ttctgataaa
     6121 atgatcgaat gttcattgta ataagcataa ggggaatatt ggaatcccca gctttcaccg
     6181 tctagattca ttcgaataat ccggtgattg gtgcgggctg gatagttcaa gcgacctcta
     6241 tatccttcgt tttccatgca aagcatgcag gtaggatagt ctacttttac cgcctcgcgt
     6301 tctgctgcga tttgttttgg atctttttct ggcttagata aattgattgt gatttccagt
     6361 tcaccatact ccgactctgc cggaaatttg atgttttttg cgatggctct tgttttgatg
     6421 tagtcatttt tcttacttaa attaaagaaa tactcagttg cttcctctgg gcttttcgaa
     6481 tagtgctgtg caaagaatgc attgacgact gaaggtggcg gtgtgagaaa atccattaat
     6541 tgcgcttcaa aaatctcttg ttctgctggc aattctgaaa gaacaccatt ttctcttgcc
     6601 ttagcgacaa gctgatccaa taagtctaca gattcttcct taactggtct tacttcaatg
     6661 ctttctagag catcttctcc taccatccct aaaagacgat tctgtaaata taagcggtcc
     6721 atttccatcc atccgccagc ttcgatcgca agtgtggcaa aatcacaaat cgtttgactg
     6781 attgacatat ctcattcccc tattcttcat aaccatttgg atggctgaca tgccaatccc
     6841 atgctgtttt gataatcgct ttgatatctg tgtattcagg tttccagccc agaatctctc
     6901 tcgctttttc acttgaagcg actaatcggc taggatctcc agctcttctt ggtgctattt
     6961 ctgctggaat ttcttttcct gtcacttctc tagctgcttc tagcatttcc ttgactgagt
     7021 atcctttgct gctgcctaaa ttgaagaagt tgctttcatt tccttctttc aagtattcca
     7081 aagctaaaat atgtgcagca atcaaatctt ctacttgaac ataatctctg atacatgttc
     7141 catctggtgt atcataatcg tctccataaa cagccaaggc ttttctttga ccaagagcta
     7201 cttgtaaaat gattgggaca agatgcgttt ctggtgtatg atcttctccg atagatgcat
     7261 ctgcttttgc tccggctaca ttgaagtagc gcaaagcgac atagcgcatg ccatatgctt
     7321 ggtcacacca cttcatcatt ttttccatca tcaatttact ttcaccatac ggatttttag
     7381 gattagctgg tgtatcttct gtgattggag actctttcgg ctctccataa gttgctgcag
     7441 tagaagagaa tacgatttta ttcacgttga actcgtgcat gacttcaagt aagatttgca
     7501 taccgtatac gttgttgttg aaatacatca atggtttttc gacagattct cctactaaag
     7561 agcttgcagc aaaatggatt actccttcaa tcggttcctt ttcaaatacg gaacgtagaa
     7621 attctttatc ccggatgtct ccttcataaa aatgagcatc tggatgtact gcttgtttgt
     7681 gacctgtgag caaattatct accacagcta cttgataccc cttttgaact agctggtcta
     7741 cagcgtgcga accgatataa ccggctccgc ctagtactaa aattgtcata aaaaacactc
     7801 ctttgattag taaaattgtg tttgttgatt ctatataatt taaaaaacga agtttacttt
     7861 tatggtgaaa acaaacaaag taaaatttcg taaattcata tggtaaattt ataataaaac
     7921 atttagtaaa cattttcaag ggttttttgc aaaggtttac aaacaaacgt aaaactagcc
     7981 tttttttctc ttttgttcca taatagatat aacaaataat aaatgaggtg gattcaatga
     8041 aaaaatttgt atctggaata ttagtaggaa gtctggcaac agccgctgcc gttgctggtt
     8101 tggttgcttc tgtgaaaaaa acagtgatcg atccaattga cgaaaaagaa gcaatgatcg
     8161 aagaaaaccg taaaaaagca atgcgtaaac gcgtttctcg ttaataatta tttaaaaata
     8221 cgcatgaaga ctttctaaaa aacagataag tcatcatgcg tatttttatt tgtcagtcaa
     8281 tataagttgt gctattttat ctgcctgtct cagattccat ggatctgtat aaggtctagc
     8341 tactgctgca gttagccaat ccacaccttc aacaggtatt ccatagcaat aaaagtcaga
     8401 ctggatttcc cctgaagcat caatcacgcg attcgtacct cgctcaacat ctatagcttc
     8461 tgaatgataa cttcctgtat tatctgaaaa tgaaaaaggg cgaacaagtt tgttttcctt
     8521 caaatttttt atgacaggat tacttgaacg agaaaagctt gtttgtggca atcgagcttc
     8581 aatcaaatag cgactgctga tttctttatc ttgtagagaa ataacatagc ggttgtgctt
     8641 ttgctgaata tcaaatggcg gttcgatcaa atgaaaaata cccgcatcta tcagtgctgc
     8701 taattctttc gtccgaatga ttggcggacc gatcgttaaa aagctattta gatgagtaaa
     8761 ccatccccat agtatttctt tgtattcctt agcagaaaaa acgtgccatt caatagcctg
     8821 ccggattgga tcacgccagt cttttaatgc atcaaatgtt gaagcaaatg ctcctgtgca
     8881 attgcctttt tccgcttctt ttatctgccc ttcaataaat attcggctag cttctgaaaa
     8941 gggctcattc tgataggcaa gaggagtttc taaatacgac caatcccatc tataagggat
     9001 tagtgctgga tgctttccga gacactcctc tgaagagagg gagagaaaat catgctcaaa
     9061 atttttcttt ggaatcggca agtgatattc ttcgatgatt ttcttgtaat aaaacaactc
     9121 aacgtctttt ttcagatagt caaaaaatgt ttcgccagtc aaaaatcccg actgatgcca
     9181 ttgctccagg ttttcttttg ttagtaaccg tggccatgcc attgctccgc cttgcttttg
     9241 atttcttcct cgagggtaat aagggagccc ccttcttgat ccactgtata caatcggttc
     9301 cttgcctgat ggatgataga ccagttttcc cttttcgttc tcgaagcgcc ctcctcttga
     9361 tactgtaaac aatccgacat aatcaaaaaa acttaagcct agacctctta aaaagacatt
     9421 ttctttaggc ggtatcttcg aaagatctac atctgcagga ttagaaggaa gctgatacag
     9481 taaaacattt ttctttgcgt agtctaccca cactctttct tcttctgtct catcattttc
     9541 ccagtgaccg cttgctaaaa caagctgatc gatagaatag ctttgctgat ctgtctgtaa
     9601 aagataacca tcttttcttt tgcttacttt ttttactaaa gaataaacaa actgaataga
     9661 agctggaaat tcttcttgca actttgagaa aaaccagcgt tgatagagtc cgtataaaca
     9721 tcgagtagat tgctgatctt tttccagacg attgatttct tctataatta gttgcttatt
     9781 tttggttacc gtctgttcga tataatctgt agcaggtcct ttactccatt gaaacaagtt
     9841 gggtccctgt gttaccaatc ctccactgga caatgtttca tctgtaaaaa gcgtcacttg
     9901 ttgagaaaca gaattcatta ataactccgg tggctgattc aaccgccaaa ctcgacctcc
     9961 aggaccattg ggatcgaaaa gaaaaagttc aagagaatgt ctgtcctgat tattatggag
    10021 aatcctttct gctacactta atcctctagg tccggcacca atgatcccta ttttcatttg
    10081 catgcctcca tttcgataaa ttttttcaat aaaacagcat ggttctgatg ttcatttttt
    10141 gcaccataaa caaaagtaat gactggatgt tctttcataa gttgactgag tttaaaaata
    10201 gcttgcttct tttcttcttc gaaaatcaat tcttgctggt atttctttgc aaatgaagca
    10261 aatcgttctg gtacatggtc aaaagttttg cgtgcttcat tactaggtgc aatttctttc
    10321 aaccacagat ctaactgcgc tttttctttt gaaattcctc gtggccagaa tctatccacg
    10381 agtattctat agccatcctc tttttcagca tcaaggtaaa cacgttttaa tttgatcata
    10441 gttgtttcct tctttctacc ttttcgctta gcttatatat gtagataata gcacaaaata
    10501 aaaaaaatga acggcacaac tatccattgc gccgttcatg tgatcttggt caaaatctaa
    10561 tgattgattc ggtttttaaa gctgtcatac aatcatctgt tttatacttt tttacttttg
    10621 atttttccag accatccgtc atatccaccc ttaagtaaat aaatctcttg ataacctgct
    10681 ttacgtaatt tatttgctgt acggatgctc aaacttttct tttgatcgta aatatagaca
    10741 ggctggtctt ttcgaataga tcctaatgaa tctttcaaaa cagtgtaagg gatgtttctt
    10801 gcacctaaaa tatgcccagc atcaaacaca tctttttcgc gaacatcgat tacttgtgct
    10861 tttctcattc cttctcgaaa ttcttcttct gtcaaagtcg ttgctgaacg tttggccatt
    10921 actcgtagat agacttccca aaggatgatt gcaagcacaa tcaatagcaa aatgatatta
    10981 attacccata aaaccatgta atgcctccgt ttttttaagc aaatttcaag gctaatgagg
    11041 cagcaccaat gacaccagct tcattaccaa gctctgctaa tttgattttt gttgattctc
    11101 ttacttgcgg gaaagtaaat tctttaaaat atttctctac acggctacgt aaaaattctc
    11161 ctgctgccga tactcctcca cctaacacga tacttgacgg atttaatgta ttccccaagt
    11221 ttccgcaagc taaacctaag tagaagcata cgcgatcaac aaccatcaat gcaaacggat
    11281 cgtcttcttg agccatttca aagatatctt tacttgttac ttcttctccg ttgtctagca
    11341 ttgctttcag ttttgaatcc cctgcgtatt cttctgccaa gaaacgacct aggcgtacca
    11401 cacctgtagc gctagataca gtttctaaac atccgcgttt accgcaagta cattcgaacc
    11461 cttcaggatc cacagtgata tgtccgattt ctcctccagc tccgctcacg ccgtgaatca
    11521 aatttccgcc agcaacgata cctccgccaa cacctgttcc tagagtaacg aatacaacgt
    11581 ctggatcgtt ttctccagca cctttccatc gttcacctaa agcagcaaca ttcgcgtcat
    11641 tatcgatagc gaatttgatt cctgttcctg cctcgatcag ttttttcaca aattgaactt
    11701 ctgtccagtt taagttatac gcaccaataa ccgttccagc ttcgctgtcc acgcttcctg
    11761 gggtacccat gccgattccg ataaagtctt cggctttcat attatataaa ttcaaacgat
    11821 gattgattga ttcaatgatt tcaggaacga tatgcttacc atcatccaaa atattggtat
    11881 cgatgctcca tttttgctga atctctcctt ctggtgttaa aattgcaaat tttgctgttg
    11941 ttccacctaa atcaatccca ataatcttct tgtccattac aaatttcctt tctttcggct
    12001 ttcttcaatt cggtgctctc tttttaatat gcgccacgct gtcatgtatg tatcatgatc
    12061 aatcaaacgg ctatcataca gattacgcaa ttcgatagtc attaattcaa tgtcatagat
    12121 gcgtgcaccc acatagataa agatgccaaa ttttttgaac agctgctgta catcatataa
    12181 gctctccatc tttaagcact tccttcatat tatacaagta aaccatattt ttttaaaccc
    12241 agtaaaatac atattacaaa aagaaataca aaaactagtg tagaaagaat cctttggtgt
    12301 atcgaatagc ttgtctgttg tttcggtaga cccaaaacat tccctaacac taatccgcca
    12361 attagcccac cgacatgtcc ccaaacatct acagaggtat ccagaagacc aaaaacaaat
    12421 gtcaacacaa taaataaaag aaaatgtctg actagctgct ggatcgctgg gttatgttta
    12481 aaatgaaacc ctaagataaa caacgcaccg aacaaaccga atatcgaagt acttgcacca
    12541 ccagacagaa cattcgcttc gttaaaagca aagcttgctg tatttcccat tatcccgctg
    12601 aacatataaa tcaagaaaaa acgccaatgg ccatagattg cctcgatttg ctgccccata
    12661 aaatacagta tcagtgagtt cattacgaaa tgcatcagtc caaaatggat gaagattggt
    12721 gtcactaaac gccaccattc attgaagtga acaagataag gaccatacat tccggcataa
    12781 taagggatct caagtcgcgg aaataattcc atcatgagga agaccgctgt ctgtatccct
    12841 aaaaaagcat aggtccataa cggtcgtctt aaccatgcac gcaattttat ttgctgttga
    12901 taattcatga aaacctccca tttcatttta gtcaataaac aattgttgga tcttctgatc
    12961 aaagctctcc ggttgccatt gttcattcaa ttgttcacga aaaacaaggc tacatgtttt
    13021 tttatgaaaa ttatttaaat accggtcgta ataaccaccg ccaaaaccga ttcgataacc
    13081 ggctctattg tagataatac ctggtacaat gaggagatcg atcatctccg gcaaatagag
    13141 cttctctgat tgtggctcaa gaacaccaaa actcgaacgt acaagaacgg actgttcatc
    13201 catttcataa aacgccatct gacgtttcgg caaagtttta ggtacaacta ttttcttttg
    13261 ttctttttct gctcgttgga taaccggctg tgtatccagt tcaaattcat tcgataaagt
    13321 cgtccctatc acttttgctg acttccagta ctcgctggcg aatagctgtt tatagattcg
    13381 ctgttctttt tcctttttct tgccactttt ggccagattt ttcagctttt cgattccttt
    13441 ccggcgtaat cttactttca attggtccaa aaactcaccc ttccttctct cataaaatgg
    13501 tataggaaaa aaagaaaaat agcaatcgaa ctttcaaata aatgcataaa tagaataaaa
    13561 agaagaccta gaaatgattg gccttccttt tttaatgaat ttcaactgtt cagcgcgtgt
    13621 ttttgtctga taaaaagtgc atacagaaaa tatggcgcac caaaaagggc aatcaaaatt
    13681 cctgtaggta cttccccact tggtagaatc actctagcaa ccatatctgc actaagcaga
    13741 aaaatggaac cgaacaaacc gctcagaaca aaggtccagt tattttcttt acgtacgact
    13801 agcttcgcag catgaggagc tagtagtcct acaaaagata aactacctgt caccgctaca
    13861 ctaccgcttg ccaaagcaac agctaacaac aaaaggattt tttgactttt agccacgtta
    13921 aggcctaatc cttgcgcgcg ttcttgacca aatgatagca atacaatcct ctgtgcataa
    13981 agtcctccta gcggaattaa gataaccgcc catggaagca atgcccaaac ataaggccag
    14041 ctcgcacccc agattgatcc cgctaaccat gcattcacaa atccataatt ttccttagat
    14101 aattggatcg tgcatagcaa tgttaatgca gaaaggccgg cattaacggc aataccggac
    14161 aataagattc gattcatacc caaaaagcca tttcgatcat aggccgcaag atagacacag
    14221 caagcagcta aaacaccacc tagaaaggct acaactagca atagaaacgg cagcgaatag
    14281 tgcgaaaaga aacctaaata aagcatcact aaaaatcctg ctccggcatt aatgcccaat
    14341 atactagaat ctgccaattc atttcttgtt actccttgca agatataacc agaaacgcca
    14401 agacaagctc ctgctaataa actgatcagt aatcgaggaa ctcgaaattc tctaacaatc
    14461 agcgccaaag aagatgattc tttccctatg aacgtggcaa aaagctgatc caggtaaata
    14521 aaaggcgtac caatcattaa actacctata gacaggatga caagtacaaa aagcaagaaa
    14581 tagaacgtcc aatatatttt tttcaccgtt gttccctcct tacttgtatc agtaaaaacg
    14641 gaacgccaat caatgcaatg actaatccaa aaggtgtttc aaaaggggga ttgatcatcc
    14701 tcgctgtaaa atccgccaac ataaccaatc caccgccaac taaaaaagag acaggtagaa
    14761 cataacgata gtccgttcca accattccgc gaacaactgg cggaaccaac agcccaataa
    14821 atgaaacagg tccgactaac gaaacggcaa tggctgctaa cactgcaact agacagaaaa
    14881 caatcaaccg cgttcgttta ggatgacttc caagagctaa tgcatgtgtt tcactcaaat
    14941 gaatcaaaga tattgctttt cctaatgaaa aagaaaccaa aacagcaagt atatataaag
    15001 gaaacaatac tgctaattgt ttccatgtga tattggcagc tccgcctaca aaccaaaaag
    15061 caagatcctg ctgcagatta aagaattggg taagtgcttg gctgatagca gtaaaaaaag
    15121 aactaatagc tacgccagct aaaatcagct gtaaaggatt catgcccatt ttcgaaaact
    15181 gcaaaaaacc aaatacgagc aaagttacag caaaagaacc tgccaatgac attagaaaca
    15241 cagaaaaatc agaagcttta ggccaaaaag caaaactaag ggccattgcc aacgaagcac
    15301 ccgcgttgat tcctagtaag cctgaatctg ctaaaggatt tcttgtcact ccttgcatca
    15361 atgcaccgct caatgcaaaa caactcccta caagaaatgc tcccgccaca cgaggcaaac
    15421 gaatatttcg tatgatttgg tgactttgtt ttgatctatc aaaatcaaac aacgcctgcc
    15481 aaaaatctgc tttttccaca gtcgctgcgc catttgaaag agcaacaaaa aaaataatca
    15541 aaagaacaaa acagaccaaa agcaggagtc tatttttctt catagtcctc ataattgatc
    15601 ctcctgctct tttggcaaca aatcataggt tagaaaaact ggtttatttg ttcttggaag
    15661 cgtgacaatc gttgcatcaa tctcaaatat ttttttcata cactcttttg tgatcacttc
    15721 ttcaggtgta ccttctgtga tcaacttacc gtttttcatt gctaataaat aatctgaaaa
    15781 acgtgaagca tgattaatgt catgaatcga catcaagatc gttgtttgtt tcatttgatt
    15841 gatttcagtc aataactgta ggatctctaa ttggtgtgcc ggatctaaaa atgttgtcgg
    15901 ttcatccaag atcaagatat ctgtttcctg agcaagcgcc atagcgatcc agacacgctg
    15961 ggtttgtcca ccagacagcg ttgcgatatc ccgcatacga aatccagcta gacctgtagc
    16021 atccaacgcc cactcaattg cttcatgatc ttgcgtgtcc atgccactga acagtttttg
    16081 ataagggtat cgtccgtaag atactacttc ttctactgtc atacctgtag ggtgttcact
    16141 agtttgtgct aataatgcaa ttttttttgc aagttctttt gaactgtatg tagagatatc
    16201 agagttattg acaatgatct ttcctgattt cacaggtagt atacgggtaa aacttttcaa
    16261 taaagtagat tttccacttc catttggccc aatcaaacta gtgattttcc cttcaggaat
    16321 cgtaacagac atgttttcaa tgattttttt gttttcgtag ccggctgcaa tgtcagctgc
    16381 tcttaatctc gtcatttcac ccatcctttc gctgaaaatc attctcaatt atgacgctgt
    16441 ttcaacattt cgtcaagcgc tactttcata tctatagcca aatgaagcgg ttgacagcgt
    16501 tcgaaatatc aggaataata accaatgaga aagattctca attatgttag gaggaaagaa
    16561 atgaaattaa aaacaaaagt attcacatta tctagcatat tgctgctggc tggagtactg
    16621 agcgcttgct ccccctcttc agataaagaa acagctacaa ctgccaattc gacgacttca
    16681 tccaatagta ctcatacagt cacagataca cttggacata aagtagagat tccaaacgcg
    16741 cccaaacgga tcatcggcag ttacttagaa gactatctga ttgcactagg ggaaaaacct
    16801 atcgcccagt ggactgttgg cagtggttcc attcaagatt atctgcaaga tgatttaaag
    16861 gatgtcccaa cgatttctta tgaccttcct tatgagaaag tgttaagctt tgagcctgat
    16921 ttgctgttga tttcttcttc cgctactgtc gaaggcggga aatacgaaca atatagcaaa
    16981 atcgcgccaa cttatgttgt aaaaaacgga acagatgtca catgggaaga acaactaaaa
    17041 gatgtcggca aagctttggc aaaagaaaaa gaggcagaaa aaatcatttc cgattatcaa
    17101 aaatccatca agaaaactcg agaagaacta gcagataaaa tcgagaacaa gacagctgca
    17161 gtgttgtggg tgaccaacaa ctcagccttt atggtcagca aaaatcggtc aagtggaaga
    17221 atcgtatacg acgatctgga atttggtgtt cctgatctag tggaagaggt ctccaaaaaa
    17281 gccacttctg actggtctgc tgtttcctct gaaaagctag ctgaattaga tgctgactac
    17341 atcatccttg tgaacagtga caaagatgct gccatgttca atgaaccaag ctggaagaat
    17401 ctaaaagcag tgacagacaa tcatgtactg caattcggac ctgaatctag ttggctatat
    17461 aacggaccta ttgccagtca ggaaatggtc aatgatatca aagaaaaact aaattgattt
    17521 taactaaaaa aagcatcttc acgattagtc gtgagatgct ttttttctta ttaacaaact
    17581 agacgcatag attttattct tcttttgaag caaccctatt ttttactctt tcccattttt
    17641 gaaaacgatg ggcatataga ttttcttgat ccgttatccc ctcatactcc tctataaggg
    17701 cgaagtctga aaaatcaata ttcccaatat acgtatcgcc ttcaaacgtg ctatcgatca
    17761 atgttcgcca gatccgacca gtgtaaggaa gaagttcttg aaaaatccga ctaccacccg
    17821 aaacatagat atcttctgag atcgtttgac tatacgacaa aagttcatcg atcgaatgca
    17881 ttacttccgc attttctttc gtgcgaaaat ttggttgcgt tgtcaacacg atgatatggc
    17941 gataaggaag tgaaagatcc cccattcctt cataggtctt tcttcccatc actaaaattc
    18001 gatccattgt atgttcgcga aagaaacgca tatcatttgg caatcgccat ggtaacaaac
    18061 catttttgcc gatcagacca tttttatctt gtgcccacat ggaaataaac atattatccg
    18121 ctccttatga ttttagatgt tttgagataa aaaaagtctc aattctttga ccagcaagga
    18181 attgagaact ttaaaaacat tattttgttt cacggtgcaa agtaactttg cgttcgcgtg
    18241 ggcaatattt ttgtttttcc aaacgatcag gattgttacg tttgtttttg cttgttagat
    18301 agttacgttc tttacaagaa gtacactcta aagtgatgtt tacgcgcatg tttttccctc
    18361 ccatttttac aagaatcctc taagatgact ctcagacttg attatcttac catgattttt
    18421 tctagattgc tagtgttttt cacgattttg ttaagtaaaa ctacttgacg ataaatacaa
    18481 cggttttact tctcaaacaa aatctttttc tttttgtata aataccctta cacttgttta
    18541 tatagattat gttttttaat aaagggggat actaatatgc catggaacat gaaggacttt
    18601 cctgcatcaa tgaaaaactt agataaattg acaagaaaaa aagcaatcga catcgcaaat
    18661 gcattattag atgaagggta ccctgataac cgtgcgatcc caattgccat tgatcaagca
    18721 aaaaaatggt atgaagatgc tagtgaatca gaaaaacgaa ctttcgaaaa agaaaaaaat
    18781 ccaagcaaga cggatgaaca cgatacgaat cctcgtgctg gaaaactgct tgattcagat
    18841 gtgatcgtag aatatgagga agaacaatgg atcgtgaaat ctaaaggggc aaaaaaggca
    18901 agcaaccatt ttgatacaaa aaaagaagca atcgaaaaag gaaagcaagt agcacaaaac
    18961 aaagaatcaa ctttgttcat ttataaaaaa gatgggacaa aagaaaaaga aatcgattat
    19021 tgaacatatg taaaggggtt gtgacgaaaa tcgtgtcaca acccccttat gttcgaaaca
    19081 atggtgttcc tctgccaact ccgaaccata agtcataata tctataaaac gggaagagct
    19141 gttttttgaa attgtttctt atcactcagt gttaaacggt aggagaaaac ctctttttta
    19201 ttgatatttg attttcatca aacagtcaca tagtcgattg cttctgcttg cggaatcgtt
    19261 tcatctacaa agtagacttt ataccatgtc aagaaactga agattgtcca gatttttctt
    19321 tgctcttctt tacgtccttc gtgatggtca tctagcaact tcatgatttt ttcttgatca
    19381 aaaaattctt ttgcaaaatc tgcttcgaat aatgctttga cttgttcgta gccgtgttct
    19441 tctttcaacc aagctttgat tggcacaggg aatcctaatt tcacacgatt cgaccattct
    19501 tccggaagat gtttgttcgc agcttgtctg aaaatatctt ttgtgttgtt ttgattcaat
    19561 aaaaacttac ttggtatttg ttgagccaat ttcatgactt cgatatccaa taaaggtact
    19621 cggacttcca aagaacttgc catagaaagt ttatctgcct ttaacaagat atcttttggc
    19681 atccattgat gaagatcgac atattgcatc ttattgactt catctttgat tccttctgtt
    19741 ttctcgtaat gaacagtcat gatttcatcc acagatggtg ctgtttgata ttttggtgtt
    19801 aacacttctg atgcttctga ttcttcgaac actcgagcat gaccgataaa atattctttt
    19861 gcaggagcaa gtgattcata taaatggatc cgtccatgga agtttttcat ttttccgatt
    19921 ttacgtccta aattatagcg tgttcctttt ggtaattttt tcaatccttg agcaatcaca
    19981 cggatgaatt ttgaattcgt atggaatcca tacgtttggt atcccgcaaa cagttcatcc
    20041 gccccttctc cagattggac tacccgcaca ctttgtgaag caagatttgc taaaaagtac
    20101 aaaggtacgc aagaaggatt tgaatcaggt tcatctaagt gatactggat caaagggaat
    20161 gctttgaacg acatgtctcc gtcgatcact gctgctgtat tatctaagtc caatagttcg
    20221 gttagttttc tggcttcaat cgcttcatta taggttttat catcaaaccc gattgagaaa
    20281 gaatgatctg gtttcaatac agaggttaca taactggaat caaccccgct ggataagaaa
    20341 gaaccaactt ctacatcact gaccgtatgt gcttcaatag aagcttgaac ggtttcgtcg
    20401 attttttcga tccattcttg tcgggaatgc gtttcttttg tttcaaaatc tgcatcccaa
    20461 tattgctgaa tgtctatttt tccatcttta tacgtataat aatggccttc aggcaaacga
    20521 tagacatctt tgaagaatgt ttcgccgttt aatggagaat attggaaagt catataaggt
    20581 ttcaacgctt ctttgttcaa ttctttgtta aaatcaggat gaggcagaaa acttttgatt
    20641 tctgaaccaa acataaatgt tccgttcatt tctgcataat aatatggttt gatcccaaag
    20701 tggtctcttg caccgaataa ctcattcttt tcattatccc aaatagcaaa agcaaacatt
    20761 ccgcggatct tttgcaataa ttctgttccc cattcttcgt aaccatgcaa aaggacttct
    20821 gtatctgcat gtgtttgaaa aacgtggcca gctgcaatca attcttctct caaaggctgg
    20881 taattataga tttcgccatt aaaaataatg atctttgtcc gatcttcatt gtaaataggc
    20941 tgagtaccgc cctctaagtc aataatactt aatcttctga atcctaaagc aacatgttta
    21001 tcgatatatt ctcctgaact attaggtcca cggtgaacga ttcgatccat cattgtatta
    21061 ataattgttt ttttgttgtc cttgtcgtta acaaaaccta caataccgca catttaactt
    21121 tcacatccta atataaaatt actgtttatt tcattcatat gcatcttatc atagaataaa
    21181 tagcggacga atattttctc tcttacaatt tcgtcacgtg aaaacaaaag atgaaagttt
    21241 ctgtttttac agaaaataaa gtcccaatat ctaatttacc atgttttctt ttttgaggct
    21301 accttattct cttcgtgtta aaaaaatatt agtggatgct atttccaaaa aaaccagcaa
    21361 aaaaagacac gacctaaaga tcgtgt
//
LOCUS       DADVXU010000047        21180 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.049, whole genome shotgun sequence.
ACCESSION   DADVXU010000047 DADVXU010000000
VERSION     DADVXU010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 21180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.049"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            56..1717
                     /locus_tag="KB953_001598"
     CDS             56..1717
                     /locus_tag="KB953_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBB6866576.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1787..2149)
                     /locus_tag="KB953_001599"
     CDS             complement(1787..2149)
                     /locus_tag="KB953_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HBB6866577.1"
                     /translation="MKEQSNEKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFL
                     WSLPFLIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSTLSSASYGMYSFWLII
                     AGILIALSAIFMLFSLLG"
     gene            2217..3158
                     /locus_tag="KB953_001600"
     CDS             2217..3158
                     /locus_tag="KB953_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6866578.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3159..4202
                     /locus_tag="KB953_001601"
     CDS             3159..4202
                     /locus_tag="KB953_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6866579.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4208..5278
                     /locus_tag="KB953_001602"
     CDS             4208..5278
                     /locus_tag="KB953_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6866580.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5268..6221
                     /locus_tag="KB953_001603"
     CDS             5268..6221
                     /locus_tag="KB953_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBB6866581.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6233..7243
                     /locus_tag="KB953_001604"
     CDS             6233..7243
                     /locus_tag="KB953_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBB6866582.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7528..8349)
                     /locus_tag="KB953_001605"
     CDS             complement(7528..8349)
                     /locus_tag="KB953_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBB6866583.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8446..8724)
                     /locus_tag="KB953_001606"
     CDS             complement(8446..8724)
                     /locus_tag="KB953_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866584.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            8992..11001
                     /gene="metG"
                     /locus_tag="KB953_001607"
     CDS             8992..11001
                     /gene="metG"
                     /locus_tag="KB953_001607"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBB6866585.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11480..12067)
                     /locus_tag="KB953_001608"
     CDS             complement(11480..12067)
                     /locus_tag="KB953_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866586.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12242..13564
                     /locus_tag="KB953_001609"
     CDS             12242..13564
                     /locus_tag="KB953_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBB6866587.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13719..14495
                     /locus_tag="KB953_001610"
     CDS             13719..14495
                     /locus_tag="KB953_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBB6866588.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14488..15057
                     /gene="rnmV"
                     /locus_tag="KB953_001611"
     CDS             14488..15057
                     /gene="rnmV"
                     /locus_tag="KB953_001611"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HBB6866589.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15050..15934
                     /gene="rsmA"
                     /locus_tag="KB953_001612"
     CDS             15050..15934
                     /gene="rsmA"
                     /locus_tag="KB953_001612"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBB6866590.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16385..17014
                     /locus_tag="KB953_001613"
     CDS             16385..17014
                     /locus_tag="KB953_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866591.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17138..18247)
                     /gene="ugpC"
                     /locus_tag="KB953_001614"
     CDS             complement(17138..18247)
                     /gene="ugpC"
                     /locus_tag="KB953_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HBB6866592.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18456..18878
                     /gene="mgsA"
                     /locus_tag="KB953_001615"
     CDS             18456..18878
                     /gene="mgsA"
                     /locus_tag="KB953_001615"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBB6866593.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19201..19725
                     /locus_tag="KB953_001616"
     CDS             19201..19725
                     /locus_tag="KB953_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HBB6866594.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKIWLFVK"
     gene            complement(19775..21076)
                     /locus_tag="KB953_001617"
     CDS             complement(19775..21076)
                     /locus_tag="KB953_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBB6866595.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT         6826 a         3821 c         4338 g         6195 t
ORIGIN      
        1 aacaacttca gaaaatatta attattctga aaattccaat aaaggggtac gtaaaatgaa
       61 aaagaaattt acatttggtt tggttgcggt atgcggcctt gtacttgctg gatgttatgg
      121 cggcagttca gccacaagca gtagttcagc aaaaagcggt tcagcagatg gaggaggcgt
      181 atttaactta gtcgtgcctc aagaaatgcc tacagctgac ttatcagttg caacagatac
      241 aatcagtttt acagcattga ataacgttta tgaaggaatt taccgtcttg atgaagacag
      301 caaaccacag ccggcaggag ctagcgagtt ggcagaagtc agtgaagacg gattaactta
      361 caaaatcaaa ttacgagaag acgcaaaatg gtcaaatggt gatcctgtta cagcagcgga
      421 ttacgtttat ggctggcaac gaacagtaga cccagctaca gcttctgaat atgcttacct
      481 gtatgcgcca gttgaaaatg cagaagacat tacagctgga aagaaagata aatcagaact
      541 agggatcaaa gcagttggtg actatgaatt agaaatcaaa ttgacgaaac aaacaccata
      601 tttccaatat ctgcttgctt tcccatcatt tttcccacaa aaacaatccg tagtggaaga
      661 aaatggggat gcctatgcat ctgcaagtga taaagctgtt tataatggac cattcacatt
      721 agaagatttt gatggaccag gaacagatac agaatggact tataagaaaa atgatcatta
      781 ctgggacaaa gatacagtaa aactttctga aatcaaagtc agtgtagtaa aagaatcttc
      841 tactgcattg aacttattca aagatggcca agcagatgac gtgatcttgt ctggtgagtt
      901 agcacagcaa aacgcaaacg atccagctta tacttctgtt aaagaagcgc ggacaagcta
      961 tatcgaattc aatcaacgtg aaaagaattc accattcaac aatgtgaacc taagaaaagc
     1021 tatttcttat tctatcgatc gtgaagcttt agtaaaacaa gtactaggtg acggatcagt
     1081 tgtctctaca ggattgatcc catcagatat ggtaaaaagt ccaacaacga acgaagactt
     1141 tgcaaaagaa gctggaaaat tagtttctta tgacaaagat aaagcaaaag aatattggga
     1201 aaaagcgaaa aaagaattgg gcgttgattc tttagaattt gatttgatgg cttctgatga
     1261 tgattcatcg aaaaaagtaa tcgaatatgt tcaaaactca atccaagaaa acttggatgg
     1321 cgtaacagtc aaaccaacac ctgttccatt ctctgtacgt cttgaccgct caacttcggg
     1381 tgattttgac gcagtactag gcggatgggc tgctgactat gcagacccaa gcagctttac
     1441 tgatttgttt gtcacaggta attcatacaa ccgtggtcgc tggagcaatg cagaatatga
     1501 cgaagcagtt aaagcagctt catcaacaga cgctggcgat gaacaagccc gttgggatga
     1561 tttccaaaaa gcaatcaaga tcatctctga tgatatgggt gtcgttccgg tgtaccaaaa
     1621 agcagaaggt cacctagtga atgaaaaaat caaaggtatc gttcaccatg cggcaggcgc
     1681 ttcatgggat tacaaatgga catacgttga agaataatcg ttaataccgt gagcatgaga
     1741 aaaaacgagg ctgcggcaaa tgtcgcaacc tcgttctcat attcacttat cctaacaaag
     1801 aaaaaagcat gaaaatagca gaaagagcaa ttagtattcc ggcaatgatc agccaaaagc
     1861 tgtacatacc gtagctggca gacgagagcg tggaaaattc tggttttttc tttcggttcc
     1921 ttgttcgtgc aagatggacc gatcgctgaa aatgatcgaa gaaacccgaa gaaaagaccc
     1981 ataaaaaacc gccgataatc agaaacggca atgaccataa gaatagatca ttagataatt
     2041 gaatgagtgt aaaggaatgg ttgatgagat tttttattaa aataaccaga atcagtagaa
     2101 aagctactgc gtaggggata gatttttttt catttgattg ctcctttatt tttggttcat
     2161 tatctaataa tgtagcgaaa catctgtcag aagtcaaaag ctagggggaa acattagtga
     2221 atagttatat caaatatgta ttaaaacgtg tctttttcat gatcatcacg ctttggttaa
     2281 ttgcaacaat cacctttttc ttgatgcaat tgctgccggg cacgccatat acgaatcagg
     2341 aacgtttgag tcctgaaaca atcgagatgc taaataaaca agtcggtttg gataaaccag
     2401 ttatcgtaca atatgggatt tatctttcta atttgcttca aggagatttt ggaatttcct
     2461 tccaatttaa aaaccagcca gtcgcacatt tgttagctgg aagaatcgga ccatccttgc
     2521 agttagggtt acaggcaatt atctttggaa cagtcctagg gacgatttta ggaacgatct
     2581 ctgccatgaa gcaaaataca tgggcagata catcttctac actcgtagcg atattaggtc
     2641 gttccattcc aaactttgta tttgctgttt tattacaata tatctttgca atcaaattac
     2701 aagttttgcc aatcgcaaaa tgggatggct ttgtgtacac aatcttacca acaattgcgt
     2761 tggccatgtc gccgcttgct gactctgctc ggtttatccg tacggaaatg gtggaagtac
     2821 tgcatagtga ttatgtggaa ttagcaaaag caaaaggatt gagccgttgg gaaatcgcct
     2881 ttaaacatgg tcttcgaaat agtttgattc cattgatgac tttattagga ccgttagctg
     2941 tagcattgat gaccggatca ctggtggtag aaaatatctt tgctattcct ggtatcgggg
     3001 aacaattcgt taagtcaatc acaacaaatg attatcctac gatcatggct gtaacgatcc
     3061 tttactcatt tatgctgatt tttgttattt tgatcgtgga tctattgtac ggtctagttg
     3121 atccaagaat tcgtgtttca gaagggagcc gaggataaat ggaaatgata ccaaagcgtt
     3181 atgaaacagt tgcagatatc cctgcggatg aatttcttcc attacaaaaa aataccgaag
     3241 aagaacgtga acagattact gcaccttctt tgaattttat ccaagattca tggcgccgtt
     3301 taaagaaaaa caaagctgcc gttatttcaa tggttttact attggtcatc attttgatct
     3361 ctgtgatcac aatctttgtt tctcctcatg atccaactgc ccaaaacgtg gcttatatca
     3421 atttgcctcc tcgtattccg ggaatcaata tcgatggatt gaatggaaaa gcaatggttg
     3481 gtggtgaact tgttgataaa tatgctcaag caaacgtccc ttcaaacgta aatttctttt
     3541 taggaacaga cggtttggga cgtgatgtat taagccgctt atttatggga atgagaatct
     3601 cgttgctgat cgcatttatt gctgcattac tagatgtaac gattggtgta gcttacgggt
     3661 tggtttctgg tttgttaggc ggacgtgtcg ataacgtgat gcaacgtttt ttggaaatct
     3721 tatcaggaat tccaaacctc gttgtcatga ttttgatgtt ggtcgttttt gaaccaggaa
     3781 ttttttcaat cgtggctgct atggccatca caaactggat accaatggca cgtatcgttc
     3841 gtgcacaaac aatgaaatta aaagatcaag aatatgtttt agctggattg acattaggtg
     3901 aatcaagaat gaaaattgca ttcaaacata ttttgccgaa tatctctagt gtcattatta
     3961 ttcaaatgat gtttagtatt ccatctgcta ttttcttcga agccttttta agctttatcg
     4021 gtttaggtct tacgcctccg tcagcttcat taggaacgat gttgagcgaa gggtacaaga
     4081 cattcctgta tctgccttat ctattatgga ttccggcagt aactttatcg gttatcatga
     4141 ttggctttaa cttacttgct gatggattgc gtgatgcctt tgatcctaaa atgaaagagt
     4201 gaagacgatg acgaaaattt tagaagtgaa agacttagaa atctcttttg atacgtatgc
     4261 tggaaaggta cgagcgattc gcggtgtcaa ttttcatttg aataaaggag aaacattggc
     4321 gatcgttggg gaatctggaa gtggaaaatc agtgacgacc agaagcatca tgcgcttgct
     4381 gtcaagcaac gccaatatag atgctggtca aatcctcttt aaaggtaacg atatcgttgg
     4441 aaaatcagaa agagagatgc aaaaaatccg tggtaaagaa atagcgatga ttttccaaga
     4501 tccaatgacc tctttagatc cgacgatgcc cattgggaag caagtcgctg agtcccttag
     4561 aaagcataat aaagtttcaa aaaaagaggg acaaaaagct gcattagaat tgcttaaatt
     4621 agttggaata cccgaagctg agaaacgtat caacagttat ccgcatcagt tctcaggagg
     4681 tcagcgtcaa cggatcgtga tcgcgattgc actggtatgt tatccggaaa tcctgattgc
     4741 tgatgaacct acaacagcgc ttgatgtgac tattcaagca cagattttgg agctgctgaa
     4801 agaaattcag acaaaaatcg atacatcaat tatctttatc actcatgacc ttggtgtagt
     4861 agcgaatgtc gctgatcgtg tcgctgtcat gtatggagga agaatcgttg aagttggaac
     4921 atcggaagaa attttctaca acccgcaaca tccctatact tggggactgc ttggttcaat
     4981 gccgacatta gatagtgcga acgatcgatt gtatgctatt cctggttctc caccggattt
     5041 attgaacccg ccaaaaggag acgcattcta tccaagaaat gaattcgcga tgaaaatcga
     5101 tgcagaacaa gaaccaccat tttttgaact atcaaaaaca cacaaggcag caacttggct
     5161 acttgctccg caggcaccca aagtgacacc gccagaagaa atccaacgtc gctgggctat
     5221 ctttaaagag aaacaaaaac atacttacgg tcaaggagga attgctgatg ccaaataaac
     5281 tacttgaagt taaaggattg aaacagtatt tcaatgtagg aaaaaagaat gaagtacatg
     5341 ctgtaaatga catcagcttt cacatttatg aaggagaaac attcggttta gttggagaat
     5401 ctggaagcgg aaaatccact actggccgta cgatcattcg cttgaatgaa cctactgatg
     5461 gtgagatatt attcgacggt caagatataa cgaagatcaa agataaaaaa ggtttgacga
     5521 aattccggca tgatgttcag atgatctttc aagatccata cgcttcgttg aatcctcgta
     5581 tgaaagtcag agatatcatt gccgaaggaa ttgatgttaa tggactagct aaatcatctg
     5641 aagagcgtgc aaaaaaagtc gatgatctat tgagaactgt tggattgaat ccaagccacg
     5701 gtactcgtta tcctcatgaa ttttcaggtg ggcaacgcca gcgtattggt attgcacgag
     5761 cattggcagt aaaaccgcgt tttatcattt gtgatgaacc aatttctgca ctagatgtgt
     5821 cgatccaagc ccaagtggtc aatttgttgc aagatctcca aaaagaacat caattgacct
     5881 atctatttat tgcacatgat ttgtcgatgg ttaaacatat tagtgaccga atcggtgtaa
     5941 tgcataacgg cttgttatta gagatgggaa caagtgatga aatctataat cacggtgttc
     6001 atccttatac agaaagtttg ttatctgcta ttcctctacc agatccagac catgagagaa
     6061 aacgcagaag aatcaaatat caacctgaac cagatgacgg acaagtacgt caacttcgtg
     6121 aaatcgcacc agaacatttt gtctatgcaa ctgagcagga agtcccatat tacgcaaaaa
     6181 aactaaaacg acaaaaggaa tctcttctgg ttgctaacta ggagggacat agatgctaaa
     6241 agcttttaaa ttccgtatgt atccgacaga agaacaaaaa cagcaattga ttcgtacttt
     6301 tggatgtgct cgatttacct ataaccattt gctgaaaaag cggcagaaaa gttggcaaca
     6361 aacaggagta gccaattttt cgctcacacc cgctacatta aaaaaagaat acccattctt
     6421 aaaagaagta gacagtcttg ctttagcaaa tgcacagctt aatttggatc gggcttttcg
     6481 caattatttc aagggacgtg ccagcttccc aaagcttaaa acgaaaaaaa gtatgtggca
     6541 atcttatacg accaacaacc aaacccgtac gatctatctg gaaaatggct acttaaagtt
     6601 accaaaacaa aaagaactaa tcaagataaa tcagcatcgg ccagttgaag gatcgattcg
     6661 ctccgctacg atttctgctc gatataatga agaattttat gtcgcactac tttgtgatgt
     6721 ttcgccagta aaaaaagaat ctttagctaa atggatcggg atcgcctatc atccgaagac
     6781 attgatccaa acatctcgac ctctagaagt aactttacca aaatttcacc agacagaaga
     6841 aaagctgcaa catgcacagc ggaaattgaa tgtgaaagtc cgctccgctc accatagaaa
     6901 gatcaggtta gatcaggcaa gcaattatca gaaacaaaag agaaaagtaa tggatcttta
     6961 tctgaaacaa aagaaccagc gagaagatta tcttgaacaa ttatctggga aattggtcaa
     7021 acaatatgac tacttatttg tagaatcttt tccaaaagaa gaggcacatg ctgatttttc
     7081 tattcacgac tggcataaat taataacaaa attgcgttat aagtcccaat ggtataataa
     7141 aaaatttctt ttgatcaata ccgacggagc agaagaaagc aattccgtaa gaaagagcca
     7201 agtagtagaa aaaatgggca gacattcggt tatcaaggga taaaaaatga atgaaactga
     7261 taggagcgtt agggttagcc ttgttaataa aaagtcttaa tgacttttgt ttctaggaat
     7321 ttttcttttg aagaaaagct cactaattat gacaaaccaa taaaacaata gcagaaaaag
     7381 ttcgtacgag ctttttctgc tattgttaat tttttattca gaaagtttac gaatacatca
     7441 aaattctgaa acaaaaaacg taactcacaa agagttgttt ttgcgttttt tcttgaatga
     7501 ttaggtctga atcttttgct cacaacctta ttcatggtgc cccggcttga gctgatagat
     7561 ttttgaagga cggccgattc ctacaggacg ttcaccgatc tcatcgaaat attggagcat
     7621 agctttctta aaattggaat gatcgatcgt tttataatcg atccctaaga atttagcaaa
     7681 tacttttcga gcttccgtaa tcgtgaaatc tttacctaaa acttgtagaa cttgcggctc
     7741 gtgttccatt ttattgacta cacggttgaa tgctttcaag atgatttgac tatggtcaaa
     7801 agccaaagta tctttgccag aagactctcc agtgactaaa tcaagcgaga tagccacttc
     7861 tccgctagat aaatggatct tgttactatg acgttctagt gtgaaccagc gtacttcctt
     7921 ggcatcatct ccggcgatta gcggttcttc tccaataaag gctaagtagc tgactgtgac
     7981 gacccagcct cgcggatctc gattaggcgt actgaatgta tgcagttgct caatattttc
     8041 ttttgaaata accacaccag tttcttcctt tgtttctctt aagacgcttt ctccagttga
     8101 ctcatccttt tggacaaagc caccaggcaa tgcccatgaa tttcgataag gatgcccttt
     8161 tctttggatc aacaaaactt tcagctgatc ttcttctttg ttatagcaca tcagtacgat
     8221 atcgacagtc aaagagggct tgtcatattc tggacgttcc tgtttttgat accagttaag
     8281 aaattcttct tcacttgctt tatgttcata atataatttt tcttcttctt tagaagcaaa
     8341 acggttcatc tgaccacctc cattgacagt aaaagtactt tttaccttat tgtagcagac
     8401 tctgaaaaaa aaccaaggat ttacttgaaa aatctaggtt atcatttaaa tatgaaaaat
     8461 aaatggaagt atgagattga ataaaaagaa aagcagataa aacggcaata ctttataccg
     8521 ctcttcacga atggcaaaga agccacaaat aacgatatat acgtcaatga taaaacgcaa
     8581 aatccaagct gctgtcataa aactgttcaa atgcccatgg aggttaaaat aaccatacat
     8641 acgaatataa aaatagctga atattaaatg tgctaatgaa agaaaagcaa tactaagtgt
     8701 aaaaaaaaga tgtttcttct gcatgattgt tccagtccct tactaagata gatagtcttt
     8761 attataccac gtgtgagggg agaaataaaa agccgttttt tagtagatat gaaacaaaac
     8821 aaaaacccgc atttattcaa cctattcatc cttttaaagg atagattgaa cgtagataga
     8881 agtcgtttat ccagtctttt tcattttgaa aaaaagctaa cagcatgctt tttttatggt
     8941 atactagcaa ggaaacgaat tcgcatcatg cgataaagga ggaagagccc cgtggcagaa
     9001 aaggaaactt tttatatcac tacgccaatc tattatccaa gcggcaaact tcatatcggc
     9061 aactcgtata ctacgattgc atgtgacgca attgctcgtt ataaacggtt gatgggcttt
     9121 gatgtgtttt atctcactgg agtggatgaa catggtcaaa aaatcgaaac aaaagcagaa
     9181 gaactaggtg taaaaccgca agaatatgta gataaaatgg cggctgatgt caagaaatta
     9241 tggaaaacgc ttgatatcag ttatgataaa ttcattcgca cgacagatga ttaccataaa
     9301 gcagctgttc aaaaaatatt tgatcgttta ttagaacagg gagatattta cttaggtgaa
     9361 tatgaaggct ggtactctgt ttctgacgaa gaattcttta cagaaacaca acttgcagaa
     9421 gtgtacaaag atgacgaagg taacgtgatc ggtggtaaag cgccaagtgg acacgaagta
     9481 gaattggtaa aagaagaatc atacttcttc cgcatgagca aatacgcaga ccgtctattg
     9541 gaatactatg aagaacatcc cgaattcatc cagccagaat ctcgtaaaaa cgaaatgatc
     9601 aacaacttta tcaaaccagg attagaggat cttgcagttt cacgaacaac ttttacttgg
     9661 ggagtaccag tcaagagtga tccgaaacat gtggtgtatg tatggatcga tgcattatcc
     9721 aattatatta ctgcactagg atatggctcc gaagatgaaa gtctattcga aaaatactgg
     9781 cctgcagatg ttcagatggt cggtaaagaa atcgttcgct ttcatacgat ctattggcca
     9841 atcatgctga tggcccttga tttgcctcta cctaaaaaag tatttgggca tggctggcta
     9901 ttgatgaaag acggaaaaat gtctaaatca aaaggaaatg tcgtttatcc tgaaatgctt
     9961 gtggaacgtt atggtttaga tgcactccgt tattatttgc ttcgtgctgt tccgtttggg
    10021 tcagatggtg tctttacgcc agaagatttt gtttcccgtg tcaattatga tctggcaaat
    10081 gatctaggga acttattgaa tcgaacgatt gctatgatca acaaatattg tgatggactc
    10141 gttcctgact atgcctcact tgtcactcca tttgatagtg aactgtctac gacggctgct
    10201 aacgttgtcg gacgttacca tgatgcaatg gaaaaaatgg aattcaatac agcattggct
    10261 gaaatctggg tgctgatttc tcgtgcaaac aaatacattg atgaaacaga accatgggta
    10321 ttagcaaaag acgaagaaaa gaaagctgaa ttgaatagtg tcatgatcca tttagctgaa
    10381 tctttgcgta tcgcagcgat attacttcag ccaatcatga cagaaacgcc agagaagatc
    10441 ttcaatcaac taggattgga ctctgaaaca atgaatcttg aaggattaca ctttggcgaa
    10501 tttccttcag gaacaaaagt agttgcaaaa ggtacaccaa tcttcccacg tttagatatg
    10561 gaagaagaag ttgcttttat tcaagaaaaa atgtctgaag gtactcaaac aaacgaagat
    10621 acagtcaaat gggatccaga agaaacagaa cttgtttcaa cgaaagaaaa acaaatcaaa
    10681 tttgatgtat ttgaaaaagt agaactaaaa gtagcagaag tcatcaactg tcaaaaagta
    10741 gaaggggcag ataagttgct gcaattccga ctagatgctg gagacagcca agaccgtcaa
    10801 atcttgtcag gaatcgctga attttatcct gatccaagcg aattgatcgg gaaaaaagtc
    10861 gtcattgtag caaatctgaa accacgaaag atgcgtggac aaatcagtca aggaatgatc
    10921 ctttctgcag aagcaccaga tggctcttta caagtaatcg aagcaccaaa atccatgcca
    10981 aatggctcag aaatcgcata gaaattcaaa gggggtatga cactagttgt gtcatacccc
    11041 ctttttccga aatttgagaa gcaattttcg taaattcctt cttattactt gaagccaatc
    11101 atataataca gaaacagaga actatccaaa ataaatccta gaaaaagaag tacgagcttc
    11161 gctgattttt gttaatttag aataatacta ataggaatcg taaaaacaga aaaaaagcat
    11221 aatgataaag ggtcgggcag attttaattg attcatctgg tactcttttt tattgtcctt
    11281 acattcataa agtttgaatg caatgacttt attacatcta tatttatatc taacagtagc
    11341 gttgatgtgt taattataag ttctatcact ttatgttcaa ttagtttgtg ctcttatatg
    11401 tgtgattgaa aatatagata aaaaaacaat catcggcttt gacttcgatg attgttttct
    11461 ttattttgta aaaatcctat cacttgattt tatggtaacc ttttattttt gtcttcaaac
    11521 ggcttggtaa gagagcagta agcagtgcaa atgggatcga aaagacatac cagaaaatcg
    11581 tacgtccgat accataatgt tgatagagct ttgtcaacat cacgtgtaac aagatcaacc
    11641 ctaaaagcaa ggagccagct ataaaccaat gattatccca atgcatgact tcactaagtg
    11701 ccatcactcc aaaaggatag aaataaaaat agatcccgac taaggtcatc cgccagttct
    11761 ttgtaaatgt tccgatattc aataaaaaga aaatcgctga aagcaaaata ccgataggcg
    11821 gaaaaagccc aaccaaaaag gaatacaaca tcccccacaa gatcatcaca ggacctttga
    11881 tcaatgcaac gattgcgatg aacaaagtca gcaatattag tttttgtgat aacgttgcaa
    11941 cagtcattaa tccaaataaa attaatgtga accaatgcca atgggactgc tgttgttctt
    12001 tacgggaaaa atcgacccgg ttggataaat aatctgctaa tttcggataa gaacgcttaa
    12061 aatccataaa ctgttcctcc ttcgtggaaa aggtaggttt agtatacgaa aaataaaaaa
    12121 gaaatacatc cgtcttaaga gggagttttt cactgaaagt ggagaaaaaa gaatggtaaa
    12181 ttaaaatatt ctcagttata ctgaaaatta taccaaattt caaagaagaa agaggatggg
    12241 aatgaaaggc aaactaaaac gagaaatcaa tctgtttggc gcattagcta ccgttatggg
    12301 aaccgtgatc ggtgcaggcg tcttcttcaa aactgctgct gttactgcta gtactcagtc
    12361 tacaagtttg acattgctag cttggctttt gggtggtttc ttaacgatct gtgctggatt
    12421 gacagtggct gaattagcta cagcaatccc ggaaactggt ggagctgtaa aatatattga
    12481 agctgcgtat ggaaaattgc ccagcttttt attaggatgg gcgcaaagct tgatttactt
    12541 tcctgctaat attgctgcat tatcgattat ctttgcgaca caattgacga acttattaca
    12601 attatctacc gattatcttt tgttgattgc agtgatcacc gctgtttctg tgacagggtt
    12661 gaatttgtta gggacaaaag tcggtgcttc tgtacaatca gtgacgttga ttgttaaatt
    12721 aataccaatt gctgtgattg tcatatgggg attattgacc ccaggacaag gaactgtcca
    12781 gttatttcca atcgaagctg gaaaagatgt tacttttgtc gaaggattga gcagtgcgtt
    12841 gttagcaacg ttgtttgctt atgacggctg gttaggtgtc ggtgcaatgg caggggaaat
    12901 gaaacggccg gaaaaagatt tgcctaaagc aattattctt ggtttgagtt ttgtaacagt
    12961 cgtttatcta ttgatcaatt ttgttttttt gaaaactttg ccgattgatc atcttgccgg
    13021 taatttgaat gcagcttctg aagcatcaga cgtcattttt ggaggaatcg gtggaaagct
    13081 ggtcactatt gggatcttga tctcggtata cggtgcattg aacggttata cactaactgg
    13141 gatccgcgtg ccttatgcga tggctttaga agacgagcta cctttcagta aacagctgac
    13201 aaagctttca aagaagttta cggttcctta tgttcctgcg gttttccagt tagcagtagc
    13261 ttgtattatg atgagccttg gttctttcga ctttttaaca gacatgttga tttttgtgat
    13321 gtggctattc agtttactga tttttatcgg tgtctttatt ttaagaaaaa aagcgccgga
    13381 attgccacgt ccatataaag taccacttta tccaatcgtt ccgattattg cgattctcgg
    13441 tgctattttc attttaggta tgacgatgat gacgcaaacg aaattagcat tgatcggtat
    13501 aggtgttacc ttgataggta tcccagtcta ttatcaaaaa aagaaaaaac gctcagaaga
    13561 gtaaaatatt tcaacaacag acagaaaaag tacgaactag gtcctctaga tcttcgtgct
    13621 ttttctattt caataaaaca agttcttcta ttttgatttt tgctacttct gcaagtagtg
    13681 cttatgtgct aaagtaagaa tgaaaaggag agataaacat gatatttgat tctcataccc
    13741 atttaaatgc tgaacagttc aatgaagata ttccagaaac gattcagcga gcacaagaaa
    13801 tgggggtaac gaaaatggcg gtcgtgggct tcgatacgcc aacgattgaa aagtctttgc
    13861 aattaagcca tgactatcca aatatataca gtattatcgg ctggcatccg acagaggctg
    13921 gcagttatac gaaagaaatc gaaaataaat tacaagaaca attgacttta ccaaaggttg
    13981 tagcattagg ggaaattggc ttggattatc attggatgga agacccgaaa gaagtccaag
    14041 ataaagtatt tcgcagacag attgctatcg caaaagaaat gaacctacca atcagtattc
    14101 atactagaga agcacttgaa gacacgtatc aaatcctaaa agaagaagat gttcgagata
    14161 tcggtggaat catgcatagt ttcagtggtg attatgaatg ggcaaaacga tttcttgact
    14221 tagggatgca tatttcattt agcggtgtcg tcactttcaa aaaagcatta gacgtccaag
    14281 aggctgcagc aaatgtacca atcgaacgat tacttgtgga aacagatgcc ccttatctgg
    14341 caccagttcc ttttagagga aagcgaaatg aaccaggcta tactcgttat acagtagaaa
    14401 agattgctga acttcggaat cttccgacag aagaagtggc tgcccaaaca tggacaaatg
    14461 cgcaccgatt atttggtcta agcgaacatg gataagatga agatccaaga gattatagtc
    14521 gttgaaggaa aagacgatac aagaaggctg cgggaagtag ttgatgcaga tacgatagaa
    14581 acaatagggt ctgcaatcaa tgaagagatc ctgatgcaaa ttgaacatgc ccaagagact
    14641 cgtggagtga tcgtttttac cgatcctgat ttttcaggag aaaaaataag aaaaatcatt
    14701 atggaagctg ttcctcaggc aaaacatgct tttttgccgc gcacacaagc taaaccaaag
    14761 aaaaagggaa gccttggtgt cgagcatgca agtgatgctg ctattttaga agcattgaaa
    14821 aaggtcgtga cgccagacat ttcccaaacg gctgtttctg aaatcactag acaagacctt
    14881 ttagactatg gtttaatagc tggggcacaa gcaaaagaaa aacgagaaaa actaggtgac
    14941 gagttgcgca tcggttacac gaacggcaaa caattggaaa aacgtttgaa gatgtttcgt
    15001 attacgccga aagaactcgc ccaagcaatg aaaatagtgg aggaacaaca tgagtgaata
    15061 tcgtgaaatt gcgacaccct ctagaacaaa agagatttta ttgaaacatg gtttttcatt
    15121 taaaaaaagt ttaggacaaa attttctgac tgaaccaaat attttaagga aaatcgtaga
    15181 aactgcgcaa atagatgaac atacgaacgt gatcgaagtt ggaccaggaa tcggtgcatt
    15241 gacggaacaa ttagccaagc atgcaaaaca agtcgtagca tttgagatcg atgatcgttt
    15301 gatccctgta ttggctgata cgatgcaacc ctatgacaat gtgactattg tacatcaaga
    15361 tattttaaag accgatttgt caacagctgt tagagaaaca tttcatgagg agcttccgtt
    15421 aaaagtcgta gcgaatttac cttactatat tacgacaccg atcatgatgc attttcttga
    15481 atcagatttg gttgtggatg aactagtcgt catgatgcaa aaagaagtag ctgatcgtat
    15541 ctctgctgaa ccaggaacga aagcttatgg ttcattatcg attgctgttc aatattatat
    15601 ggaagccagt ttagcattca tcgtaccaaa aacagtattt gtccctcagc caaatgtaga
    15661 ttcagcgatt ttgaaactga caagaagaga tataccggca gtcgaagtga cagacgaaaa
    15721 agcatttttc cgattgacaa aagcggcatt tcagcaaaga agaaaaacgc tttggaataa
    15781 cttgcagcat tcgtatggca aagatgacca gacaaaagca tggttagcaa aaagcttgga
    15841 aacagctgga atcgatccaa aacgtcgtgg ggagactttg tcattacaag aattcgccgc
    15901 attaagcaat gcgatgtcag aaaatatgca gtaaaaaaag aggttgtgac agaagttgtg
    15961 aaaaagctgt cctgcatcaa gtaataagaa cagccaaaca aaaatagttc ctcatgtttt
    16021 tcgtttgttt gggcttatta ccgcagatgc aagcttttga acaccgttta agaaagaggt
    16081 tgtgggcctg ccgtttaact ctaagtcaca aggaacaatt tcaaaaaaca gcttctcatg
    16141 tttttgagtt agttggtctt attacttgat gcaagctttt gaccactatt atgccgaaca
    16201 aggagatgtg acacaaattt tgtcacatct cctatttttg tttcttgatc aaaaaattct
    16261 tatacttgat actcacactc attttgtatg gatttagaaa agaatatgtg tcttgacagt
    16321 tattagtaag tgagttatac taagtcgaca tttatgtgtc aagacatatt aggagagaga
    16381 aaaaatgaga aaaaattcag ttcgaaaact aacgatttct gctttattga ttgggatggg
    16441 ggtcattatc ccaatggtca tgcctaaaat catgattcca ccagcttcct ttactttggc
    16501 gtctcatgtg ccattattta ttgcgatgtt ttttacacca ggagttgcag tggctgttgc
    16561 gctaggtacg acatttggtt tctttttaac aacgccggtt atcatcgcat tgcgagcatt
    16621 gtcacatctt gtgtttgcgt tgattggggc ttggtacttg caaaagcatc cgagtatcgt
    16681 tttaaaaaat ggacagttta cttttgctaa ttggcgtttc caagggttca attttgtgat
    16741 cggtgtgatt cattctttag cagaaatgct agtagtcagc atcttttact ttattggtaa
    16801 tatgcctgaa acatattatt ctcaaggata tttttataca gtattcatct tgatggggat
    16861 tggtgggctt atccacagtt tagttgatta taatattgct tattttatag caggaacact
    16921 cagcaaatat tttgatatcc ccgtatttac ggcagcaaaa aaagaaaatc ggaaagagtc
    16981 gattcaaggg tacgcgaaga aaacgacata ttagacatga tctctcaaaa aaaaaactct
    17041 catctagtaa tggataataa atcctctaag atgagagttt tttgtcctaa aaagagctgc
    17101 accaacatct tctggcacag ctctaacagg gggcaattta tggaagtttg atgacttgtt
    17161 ctgtttcttt atcaaagaag tggcctttgc tgatgttgaa ggcaaggtcg actatttcgc
    17221 ctggtttatg gaaatctctt gcatctactt ttgagatgaa ctcagtagaa ccagtttttg
    17281 tgtaaagcat cgtttcagct ccaagtaatt cggaaacgac aacttctgat ttcactacgg
    17341 cttcaggtga agcttcaagg gctacttgtt cactgtggat gtcttctgga cgaataccga
    17401 agataacttc ttttccttca taaccttttt caactaatac tttgtttttt ccttcaggaa
    17461 tacgaagctt aagcccatgt ccatctgtga taacaccatt tttcaatgtc acattaaaga
    17521 agttcatggc aggagatcca ataaagccgg caacgaacat attgacgggt gtatcataaa
    17581 cttctttcgg tgtaccgatt tgttggataa atccgtcttt catgataacg attcggtcag
    17641 ccatagtcat tgcttctgtc tgatcgtgag taacatagat ggtcgttgtt tcaagacgct
    17701 ggtgaagttt ggcaatctca gcacgcatgg ctacacgtaa tttagcatct aaatttgata
    17761 atggctcatc cattaagaag acttttgcat cccgaacgat tgcccgtccc aatgccacac
    17821 gttggcgttg tccaccagat aaagcagctg gttttcgttt caagtattct gtcagaccta
    17881 agatctctgc tgcattgtcc acacgttttt tgatgtctgc tttatcatat ttacgtaatt
    17941 tcaagccaaa agccatatta tcaaaaacag tcatatgagg atacaatgcg tagttttgga
    18001 aaaccatggc aatatcacgg tctttaggtg ccacgtcatt catgactttt ccgccgatat
    18061 ttagttctcc ttcagagata tcttccagtc ccgcgatcat tcgcaaagta gttgactttc
    18121 cacaaccgga aggaccaaca aaaacgataa attcacggtc tttgattgat agattaaaat
    18181 cagttacaga ataattttct gcattgtcat attttttata tacgtttttc agagccattt
    18241 ctaccataat acgttccaca ctttcttttt tagttttatt aactatttaa agtataaatg
    18301 aaagcgttaa ctaacttcaa tgtcatgttg aacaagaatt ttttcttttt tttgtcaatg
    18361 tgctagtttt aagctttaat aaatatccat aagaaataaa aactgataca ctggagtaaa
    18421 taagtatgtt aacaatatca aaaggagtgt ctcttatgaa aatcgctttg atcgctcatg
    18481 accgaaaaaa acctatgatg gtgaaattag caacagcgta taaaacaata ttggaaaaac
    18541 atgaactttt tgctaccggg acgactgggc agaggatcac ggaagcaacc ggcttgccga
    18601 tccatcggtt taaatccgga ccgttaggcg gggatcagca aattggagcc atgatttcgg
    18661 aggatcagtt agatttggtg atttttcttc gagacccgct ggctgcacag ccccatgaac
    18721 cagatgtaac agcacttatc cgtttatctg atgtctatga gattccgtta gctacgaata
    18781 tcggaacagc tgaggtgcta ctaagaggac tggaagccgg cttcctagat tttcgaagaa
    18841 tcgtccatga aatagacaag catccacttt cattttgatg gataagcgta tggaaaaata
    18901 acgtttaatg ttcgcctata tgtagctata aaagctaaac aaactgataa tatgcgatat
    18961 aatacgaacg tattggataa tataaacaat aatattagtt tttcgtattt gcagtaaatg
    19021 tgaaaatatg ctatagtttc tgtgggaaaa gatcccaaaa ataatacaga gtagaaaata
    19081 aagcgttaag tgctaagagg atgggatgtt gcctcttgga cgaaggacgt aatgttcgcg
    19141 gttttatttt cgcattcgct gcatgatacg atgtagcagc tcttaaagga gatgctaaaa
    19201 atgaagaaac accggttaga cgcacgtatg attgctatta tgggcttatt gattgcattg
    19261 atggtcactt tgtcccgact ggtagcaatc gagaccccat ttattaaaat cagtgtcaca
    19321 tttattccac aagtgatcat gggcattctt tttggtcctt tttggtcagg gataggcgca
    19381 gttttagctg atttagtagg aatggccctc ttctcgaaat ctgcattctt tattggattt
    19441 acattgaatg catttattga aggagcaatc tacggattct ttttctatcg aaaagagatc
    19501 acttggaaaa atgccattct ggctacgctt agtgtcacgc tgatcatcaa cttatttttg
    19561 acaccattat ggcttgcgtt gatgtaccat gtgccattgt tcagctgggt cgtttgggcg
    19621 ccccgcctgt taaaaacagt aatatggtta ccgattcaat caattgccat ttattacgta
    19681 ggacgtagta ttccttacaa aaaaatttgg ctctttgtca aataggactg atagagtgag
    19741 ataagaacat ctaaggtaga attccttttc tactttagat gttttttgat tcataggtaa
    19801 tcagattatt tagtaagaaa attctggtct tcattttccg aaagcttcga aagccataag
    19861 cgactcgttt caatgctttg atgtgggtat gcagattttc tattttggca tttgaataag
    19921 gcaattcgag ggctaaacga atgccggatt catagtttat aaaagctttt tttatagaat
    19981 gcagttcttt tggaacggaa ttgggcatcg ttcttacaag tttaaagaaa gtgtccgcct
    20041 ctttgtcgtg aaacgcttga agaatttctt gatagtaaaa ataggcgatt cgtaattcgt
    20101 cagagatcga aagcagacga tccaccactt cactttcggt gagatacgcc caaccaaaat
    20161 tgcgccattt ggtccatttg tcataaacaa gcttgtcctg tcgtttgatc agaagccgag
    20221 acaacgcctt taattgtcga taatatttgg cttgtgttgg atcgtttcga tcaaagcttt
    20281 tcatgattcg tacgcgaata tcctcaaatt tacgaatgag atgtttgaca atgtgaaaac
    20341 gatccacaac gagttttgcc tttggaaaac attcctttat cagagaggca taagaaacat
    20401 tcatgtccgt cacgatgacc tttaccttca atcgtgcgcg tcgttcaaat tgcataaaat
    20461 aagaaacgag agcctttttc ttccgtgaag gaagaatatc cagtaatttt ccagtatcgc
    20521 catccataca agaaaaggag tagggaccga cttgattttt cagtgaacga aattcatcca
    20581 ttaataaaca acgcggtaac caatttcttt ttacttttgt catggggatc agagaagcga
    20641 gtgtccgaga aacggtcgac caagaaacgc cgcaatgctt agcaattgtc gtcattgctt
    20701 gtttttcagt tagctcttct aaaattttat agcgcacttg tttagcgata aagcagtttc
    20761 tatcgacaat gtaggtattc gccgtaaaat aggtgtggca gccacgacag tggtagcgtt
    20821 gtttcgctaa agcaagataa gtcagttggt tcgcacattt attaagttga atgagcgatt
    20881 ttttctttcc gttcttaacg atcatgcctt catgattcat ctgtttacac aaaggacagc
    20941 gacgaatttc tttacttaac gtcgcagaaa caacaagact cttgatattc ttaatgctct
    21001 tcgattccac atttgtgatg aaaatgtttt tatctttgat tccaagtagt agtttgatat
    21061 gatgaagatc aggcatacag aacgctcctt tgattgtgtt gtggtgattc aattctaacg
    21121 gagcttctgt atgttttgtt ttttcatgct caaaagggac cgatagaatg attttctatc
//
LOCUS       DADVXU010000048        21068 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.050, whole genome shotgun sequence.
ACCESSION   DADVXU010000048 DADVXU010000000
VERSION     DADVXU010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 21068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.050"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(42..230)
                     /locus_tag="KB953_001618"
     CDS             complement(42..230)
                     /locus_tag="KB953_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBB6866596.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            500..862
                     /locus_tag="KB953_001619"
     CDS             500..862
                     /locus_tag="KB953_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBB6866597.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            914..2596
                     /locus_tag="KB953_001620"
     CDS             914..2596
                     /locus_tag="KB953_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HBB6866598.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2713..4749
                     /gene="recG"
                     /locus_tag="KB953_001621"
     CDS             2713..4749
                     /gene="recG"
                     /locus_tag="KB953_001621"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBB6866599.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4798..5799
                     /gene="plsX"
                     /locus_tag="KB953_001622"
     CDS             4798..5799
                     /gene="plsX"
                     /locus_tag="KB953_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBB6866600.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5921..6163
                     /gene="acpP"
                     /locus_tag="KB953_001623"
     CDS             5921..6163
                     /gene="acpP"
                     /locus_tag="KB953_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBB6866601.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            6431..7435
                     /locus_tag="KB953_001624"
     CDS             6431..7435
                     /locus_tag="KB953_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6866602.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            7436..8380
                     /locus_tag="KB953_001625"
     CDS             7436..8380
                     /locus_tag="KB953_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6866603.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            8380..9342
                     /locus_tag="KB953_001626"
     CDS             8380..9342
                     /locus_tag="KB953_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6866604.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            9369..10283
                     /locus_tag="KB953_001627"
     CDS             9369..10283
                     /locus_tag="KB953_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6866605.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            10309..12090
                     /locus_tag="KB953_001628"
     CDS             10309..12090
                     /locus_tag="KB953_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBB6866606.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            12438..13124
                     /locus_tag="KB953_001629"
     CDS             12438..13124
                     /locus_tag="KB953_001629"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBB6866607.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            13144..16725
                     /gene="smc"
                     /locus_tag="KB953_001630"
     CDS             13144..16725
                     /gene="smc"
                     /locus_tag="KB953_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HBB6866608.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            16734..17543
                     /locus_tag="KB953_001631"
     CDS             16734..17543
                     /locus_tag="KB953_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBB6866609.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            17554..18552
                     /gene="ftsY"
                     /locus_tag="KB953_001632"
     CDS             17554..18552
                     /gene="ftsY"
                     /locus_tag="KB953_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBB6866610.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            18739..21006
                     /gene="gshAB"
                     /locus_tag="KB953_001633"
     CDS             18739..21006
                     /gene="gshAB"
                     /locus_tag="KB953_001633"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HBB6866611.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
BASE COUNT         7150 a         3749 c         4667 g         5502 t
ORIGIN      
        1 aaaaaaccgt tcactatctg aacggtccaa gtatcattca attaaacgcg ttcaactttt
       61 cctgatttca aagcacgagt tgacacccaa acttttttag gttttccgtc gaccataacg
      121 cgaacttttt gtaagttagg tttaacagta cgttttgttg agttcatagc gtgtgaacgg
      181 ttgtttccac tttttgtctt gcgacctgta aagtaacaaa cttttgccat ggtaatttcc
      241 tcctttgcct tgatttggag caatagaata attcagctca taccagacta acatatcata
      301 tagaatggaa atttgcaagt acattttcat agatacaatt gacaattgag aagaataatt
      361 gtaaattaaa caaggttata cctgaaattt tgcaaggttt ttgagtactt tgggtaactg
      421 ccttttattt caagttgcgc tatgataaaa tatgatagaa gaaaaatggg agttttttac
      481 ccgttaagga ggctcattca tggctgtaaa aattcaaacg tcagctggaa ccattgaaat
      541 taccaatgat gtaattgcaa cagttgttgg tggcgccgcg acagatgtgt tcggtgttgt
      601 aggaatggcc agcaaaaatc aaattaaaga taatattact gagattttgc gtaaagaaaa
      661 ttactcaaga ggagtagttg tccgccaaga agataatggt attgctgtag atgtttatac
      721 gattatcagc tatggtacga aaatctctga agtttcccgc aacgtccaag aaaaagtgaa
      781 atacaatcta gaggcattat taggcgtaac tgccaattca gttaatgtct ttgtacaagg
      841 tgttcgtgta ctgccagact aggcaggaga ctggctggta actcagcagt cgatctgaag
      901 gaggattatc taggtgagtg taacagaaat aagtgcaagc cagttccagg aaatggtcca
      961 ggcaggtgcg aatcgtctgc aagtaaatgc agaatacgtc aattcattaa atgtattccc
     1021 tgtgccagat ggcgatacgg gaacaaatat gaatctatct atgactagtg gagctaaggc
     1081 agtagtagat tcaacctcag aaaaagtagg agaattagca gcaatgcttt caaaaggatt
     1141 gctgatgggt gcgcgcggaa attccggcgt tattttgtct caattgtttc gtggattttc
     1201 taagcaaatt ccagatgttg tctcattaaa tgcttccgat cttgctgcag ccttcactca
     1261 tggagttgaa actgcgtata aagcagtaat gaaaccagta gaaggaacga ttttgacagt
     1321 agctagagaa gcagcgaaag ctggagaaaa gaaagcaaaa agtactgatg atgtcgttga
     1381 agtaatgaca gctgttgtaa aaggggcaaa gcgtgcttta gctaaaactc cagacttgct
     1441 tccagtccta aaagaagtcg gtgtcgtaga cagtggaggg caaggactgc tgtttgttta
     1501 tgagggcttt ttaagtgcat tgaatggaga attccaagcg gatgaaacat atgaaccttc
     1561 tccagcagaa atggatgata tggtcaatgc agaacatcat cgcagtgtcc aaggtcaaat
     1621 ggcgacagaa gatatccatt ttggttactg tacagaaatc atggtaaaaa tcggagaagg
     1681 acctacagtc gatagtacat tcgattacga agaattccgt aactatttag atggtatagg
     1741 agattcttta cttgtggtca atgatgacga gatcatcaaa gtccacgtgc atacagaaaa
     1801 cccaggagaa gtcttgaatt atggacaaaa attcggttca ttagtcaaaa tcaaagtcga
     1861 caacatgcgc ttgcagcatg agacgatttt ggaacacgat gaagaagtga acgagtttgc
     1921 gaaaaaagaa acaccacgta tcccgtatgg tatcgtagcg atcgctgctg gtaaaggcgt
     1981 ccaagagctg tttgaaagtt taggcgctaa ttatgttatc agtggtgggc aaacaatgaa
     2041 tccaagtacg gaagacattg ttaaagcgat tcaagaagta aacgcagata aagtgattgt
     2101 cttgccaaac aataagaaca tcttcatggc agctgatcaa gcagcagaag tagcagatat
     2161 cccagtagca gttgttcctt caagaacgat ttcccaagga ttgacagcta tgctttcatt
     2221 caatgatcaa gcttctttag aagaaaacaa agaaacgatg acagaaatgt tgtctagcgt
     2281 tgtgagcggt caagtcactc acgcgatcag agatacctca attgacggtg tagagatcaa
     2341 agaaggagac tttttgggca tgatcgatgg aaaaatcctg atctctaacc cagatatcac
     2401 agcaacagcg ttaagtacac tagaacaaat gatcaatgag gacacagaga tcgtaacgat
     2461 cttggcaggt gatgaaggaa cagttgaaca agcaaatgaa tttgctgaga aactaacaga
     2521 aaaacatcct gatttggaaa tcgagatcca ccaaggagat cagcctgtct atccttatct
     2581 attttctgct gaataatttc tgtgtcagaa tatctttcta acacagtatt cattcataaa
     2641 aggccttcgg ttagtgatca ggtatgatcg ctgaccaagt cctttttttg ttaagggagg
     2701 gaggaaaaat ttatgcgttc tttagatgat ccaatcacta tgctggcagg agtcggagaa
     2761 aaaagagcag ctagtcttgc cagcttgggc atccaaacga ttgaagactt actgacttat
     2821 tatcctttcc gctacgaaga tattcaggaa agaaatctaa acgagatcca agatcaagaa
     2881 aaggttactt tgaaaggaat cgtagtctcg cctcctgtga tgaatcgatt tggttataaa
     2941 aaaagccgat tgcaatttcg tatgatgcaa gaacatgatg tgttcaacgt ttcttttttt
     3001 aatcaacctt atctaaaaga aaaagtcatt ttatcggagg aaatcgccgt atacggtaaa
     3061 tgggatgcaa agcgtaaagc attgaacggg atgaagattt taggttctca aacaatggat
     3121 gacttttcgc cgatatatca cgtcaacaaa tcaattcgtc aaacgacatt agttgatcta
     3181 atcaaaaaag ggtttcaaga atacggcaca ttgattgaag aaaatctgcc aaacaacctt
     3241 gtggagaaat atcgactgct tgaccgagct tctgctgtaa gaagtatgca ttttccaaaa
     3301 gaccacgaag aaaaccatca agccaaacga cgtgtcgttt ttgaagagtt ctttttattt
     3361 caaatgcgtc ttcaaggatt gaaaaaagca gaaaaagcag aaacgaatgg tttggagatc
     3421 ctttatgatg tccaaaaatt gaaagaattt actcaaaagc taccttttga actgacaaat
     3481 gcacaaaaac gtgtaacgaa tgaaatctgt cgggatcttc gcagtccgca gcatatgcag
     3541 cgacttttgc agggagatgt aggtagcgga aaaacagtgg ttgccgcgat tgcgttatac
     3601 gctaccgtta cagcaggttt ccaaggtgcg ttgatggtgc ctacagaaat cttggcacaa
     3661 caacatatgg aaagcttgca acagctgttt gatcccaatg aagtaaccat cgcattgctt
     3721 acggggtcaa cgaaagcaaa agaacgtcgt gagctccttg aaaaactaga gcaaggggaa
     3781 attaacatcg tgatcggcac acatgcattg atccaagatg gagtcgaatt tcagaattta
     3841 ggtctagtta ttacggatga gcagcaccgt tttggcgtga accagcggaa agtacttcgt
     3901 gaaaaaggct ggcgaccaga tgtcttattt atgacagcga ctccgattcc tagaacatta
     3961 gctattacag catatggtga gatggatgta tctatcattg atgaacttcc agctggacgt
     4021 atcccgatcg aaacacgctg ggtaagaaca cctcaattgg acagtgtact ggattggacg
     4081 aaaaaagaac tggcaagagg acatcaaatg tatgtcatct gtccgctgat tgaagaatca
     4141 gaagcattag atgtaaaaaa tgcagtggaa atctatgaaa aacttcgtga actatttgca
     4201 ccaaaatatg aagttggctt gttacatggt aaaatgaaga atcaggaaaa agaagtaatc
     4261 atggaagcct tcaaagacaa ccagatgcag atattagttt cgactactgt aattgaagta
     4321 ggggtaaacg tgccgaacgc gaccgtcatg ctgatcatgg atgcggatcg atttggattg
     4381 gcacagctgc atcaacttcg tggacgtgtt ggacgcggaa gtgatgcttc ctactgtatc
     4441 ttagttgcta atcctaaaaa tgaactaggt gtggaacgga tgaaaatcat gacagagacc
     4501 accaatggtt ttgtactaag cgaaaaagat ctggaattac gtggtcctgg tgaagtattt
     4561 ggcttccgtc aatctggtct gcctcaattt gcgattgctg acttggtgac ggatggaaat
     4621 gttttggagg tagccagaga agaggctgca gccctttggc agataaagga ttggcagatg
     4681 cttcctgagt atcagccttt agcgcaaagc ctccaaatag aaaatcagga taatcgttat
     4741 ttcgattagc agactgtgct atactttata ctgatgacgg aaaaaggagt ggaaacaatg
     4801 aaaattgccg tagatgcaat gggcggagat cacgcacctc aagcaattgt agaaggcgtg
     4861 gctttagcgc agcaagattt tccagaagta gaattcttac tttacggaaa agaagcagaa
     4921 atcaagaaat acttaacaaa tgaaaaaaac atcacgatta tccatacaga cgaaaaaatc
     4981 aatagtgatg atgagccagt aaaagcaatt cgacgaaaaa agactgcatc aatggtcctt
     5041 gctgcacaag cagtgaaaaa cggtgaagca gatgctgtat tttcagcagg gaatacaggc
     5101 gcgctactag cagcagggtt gttcatcgta ggacgtatca aaaatatcga acgccctggc
     5161 ttgatgtcta ccttgccagt agtagggaaa gaaggtgccg gtttcgatat gctggatctt
     5221 ggcgctaacg cagaaaacaa accagaacat ttgttgcaat acggtatttt aggttctttt
     5281 tatgcacgta aagtccgagg tatcggacgt cctcgtgtcg cactcttaaa caacggaaca
     5341 gaagcaacaa aaggtagtga agtgacgaaa aaagcctatg aactccttca gaatgaaaca
     5401 agcttgaatt ttattggaaa cgtagaagct cgtgagctac tcaatggtgt ggctgatgta
     5461 gtggtcacag atggatttac aggcaacgct gttttgaaat ctattgaagg aacagcaatg
     5521 aatatgatga atctattaaa atctgctatt ctaaatgaag ggatcaaagg aaaaatgggc
     5581 gctatgcttt taaaagacgg cttacgttct ttgaaagcag aaatggatta ttccaaacat
     5641 ggcggtgccg ttttgtttgg cttgaaagca ccagtcatta aaactcatgg agcaacaggc
     5701 cccgatgctg ttcgctatac gatccgccaa atccacacaa tgcttgagac agatgtagtt
     5761 ggacaattag tcgaacaatt tgaaaaaagc gaagaatagt atttggatat ctgaagcaat
     5821 ccgttccatt gaaaaaaagg tggacattcc tgaaaaatgc cagtaaaatt agccatggtg
     5881 taggtaattg acaagtgtat gattcttgga ggtgaaaaag ttgactcgcg aagaagtatt
     5941 taacaaagta gcgaaaatta tttcaaatca ttttgagata gataccgata aagtgacgga
     6001 tgaattgaac atcaaagatg atttaaaagc tgattcaatt agtattatgg aattcgtttt
     6061 agagcttgaa gatgaattcg gtacggaaat ttcagatgaa gacgctgaac aaattgaaac
     6121 agtcggtggc gcagtagatt atatcagcag ccatcttaaa taatttcaaa taaaagcaaa
     6181 gaaggataaa aagctgcata cagcttttta tccttctttt tttaatcttt ttgacacgta
     6241 aaagtcattt ttctgataat tctggcgata aacagaaatt atgttgcaaa gtaaagcgat
     6301 tttctatata attattaata attcattaca caaagataag aataaccttt gttataaaac
     6361 gctttattct gtgtaatgtc cttttttatt aagaaaaact tccatttagg aagcagagag
     6421 gggtattcga ttgtccgata accaattatt agaagttcag aacctacata caggattccg
     6481 actcaaagat gactattacg atgcagtaga tgacgtttca tttactttag acaaaaacga
     6541 gattttagca atcgttgggg aatctgggtg cgggaaaagt accttggcta ctacgattat
     6601 gggattactt gatcctaata acacaaaaat tacaggtgag attttatata acgatctgaa
     6661 tttaatcgac ctgaacgaaa cgctctataa taaaatccgc ggaaatgata tcggaatgat
     6721 tttccaagat ccgttgtctg cattaaatcc tctcatgaga atcgaagacc agatcaaaga
     6781 aggactttcc tatcacacaa agatgaatgc agaacagcgt caagctcgtg cactagaatt
     6841 gctagaacaa gtagggattc ctaatcctgc gagagttgga cgccaatatc cccatgaact
     6901 atcaggcggt atgagacaac gtgtcatcat cgcaatcgca atcgcctgca aacctccaat
     6961 cgtcattgca gatgaaccga cgacagccct agatgtaacc atccaagcac agattcttga
     7021 tttactgaaa gaccttcagg aagaaacaaa aacagggatc atcttgatca ctcatgactt
     7081 aggagtcgta gcagaaatgg ctgatcgagt ggcagtgatg tatggcggac agttcgttga
     7141 agtagcaggt gtaaaagaac tgtttgatca tcctaagcat ccttatacac gctctttact
     7201 gaactctata ccacaagaag gaaatcatga tgcagaactc catgtgatcg aaggtgtcgt
     7261 tccttctctg aaaaacatgc ctcgtaaagg ttgtcggttt gcaccacgta ttccatggat
     7321 accagcaagc gatcatgaag aagagcctat ccttcatgaa gtcgcaccga accacttggt
     7381 acgttgctcc tgctataagc atttccactt tagagacgaa aaaggagagg tgtagatgac
     7441 agaattaatc acaatcaaag acctgaaagt tcattaccct attcgcagtg gattcttcaa
     7501 tcgagtaacg gatcatgtat atgctgtaga tggtgtggat tttattatcg aaaaaggaaa
     7561 gacatatggt ttagtgggag agtctggctc tggaaaatct actacgggaa aagccgttgt
     7621 gggattagaa aaggtaacag ccggacaaat catgtatgaa ggaaaagatg taacgaagcg
     7681 aagcaaccgc aaaaaaatgg gttataacaa ggatgtgcag atgatcttcc aagattcaat
     7741 gtccagtttg aatcctaaaa agcgagtgtt ggatatcatt gctgaaccca tccgaaattt
     7801 tgaacggtta agcgatcaag aagaaaagaa aaaggtaaaa ggactgctag atatcgttgg
     7861 gatgccggaa gatgcgctat ataaatatcc tcatgaattt tcaggcggac aacgtcaacg
     7921 tctaggagta gcccgtgctg ttgctaccaa tccaaaactg atcgtagccg atgaacctgt
     7981 atctgctctg gatctatctg ttcaagcgca agtattgaat ttcatgaaac gaatccagca
     8041 agaatacggc ctcagctatt tattcatttc acatgattta ggtgtagtaa aacacatgtg
     8101 cgacaatatc gcaattatgt acaaaggtcg atttgtagaa atcggtacaa gagaagacat
     8161 ttataatgac ccgcgacata tctatacgaa acgtctgctt tcagctattc cgcaaatcga
     8221 tgtagaaaat agggaaagcc acaaagaaaa tcgacgaaga gtagaacaag agtatatcca
     8281 aaaccaaaaa gaatactatg atgcgactgg tcgagtctac gatttgcgta cgatcacaac
     8341 tacacacaaa gtggcgttga aagatggagg tgcaagctaa tgtggaaaac gattctgcga
     8401 cgtgttttat tgatgatccc gcaagttatt attttaagtg ttctgatctt cttattagcc
     8461 caagcgatgc cgggggatcc atttaccggt ttgatcaatc caaatcagga tccggcagtg
     8521 atcgaacaga tgcgacaagc agctggcttg aatgacccat ggtatgtcca gtacttccgt
     8581 tggatcggaa atgctttgca aggcgatttt ggtcaaagtt tcttatacaa acgctctgtt
     8641 gctgacttga tcggcgaacg gatcatcaac actttatatc tatcgatctt gacagttgtc
     8701 cttacttatt tgattgctgt gcctctgggg atgcttgctg gccggtatca aaattcaatc
     8761 ttggataaga ttgtcgtaat ctacaacttc tttagttttg ctgttccttt atttatcttc
     8821 ggcttgatca tgctatttgt ttttggttat cgtttaggct ggttcccaac aagtggatca
     8881 caaacactag gtttgcaagg tggatttatc actcaatgga tagaccgttt gaaatatatt
     8941 ctgttaccat caattaccca agcatttttg gcgactgccg tcacaatcca atatctgcga
     9001 agtgaagtaa tcgattcgaa atcgctcgat tttgtcagaa cagcccgttc caaaggggta
     9061 ccgacgaata aagtattcag tcgacatatt ttccgaaatg cagcgttgcc gatggcttct
     9121 caaatggggt atgaaatcac ttcgttgatt gctggttctg tagttatcga aaagattttt
     9181 ggttatccgg gagtcggaaa actatttatt gattccatcg gccaacggga ctataccgtg
     9241 atcacatcct tagtattgat tttaggaatc gcgacgctag tggggacatt actttcagat
     9301 atcatcatga gtatcgttga cccgcgtatt cggattcaat aaaaccaatc agaaaggaga
     9361 atggatcgat gagtgaaaaa aacaatacag cagttcaaga aagtattcct cctatgggga
     9421 tacgaatgat tgctcgagaa tttaaaaaag ataaaattgc tattttttca ttaggtttgc
     9481 tagtggtttt attattagtg atctttatcg gtgcgatgct gaccgatcaa gacaaagtga
     9541 tgtatgttag tatcttcgac aaatacgctg caccaggaac agtttcgaca caaggacaaa
     9601 agttcattct gggagcagat gaaggcggac gtgatgtatt aggacaattg atcataggtg
     9661 ctagaaactc tgttacgatc ggatttgcga tcacgatcct tacttcgatc atcggggttg
     9721 gattagggat catctctggc tactacggag ggatcattga caacatcttg atgcggatcg
     9781 ttgattttat catgattcta ccgatcatgc tgatcattat cgtcttcgta tcgattattt
     9841 ccagatacaa tgtctggtcc tttatattca tcatgagtgc attttattgg gtagctaaag
     9901 cccgattatt ccgaagcaag acattatctg aagcaagaag agattatgtc agtgcctcaa
     9961 aaacaatggg aaccaatgat tttaaaatca tgttccgcga aatcatgccg aatctaagtt
    10021 cattaatcat tactaatttg acgatgaact ttgcggcaaa catcggtatc gagacaactt
    10081 tgactttctt aggtttcggc ttgccggcaa atgtacctag tttaggtaca ttgatcggtt
    10141 atgcaagtaa cggagaagtc ttagtgaatc ggcaatggat ctggttaccc gcatcaatcc
    10201 ttattttagt tttgatgttg agtatcaatt atgttggaca agcattcaag cgttctgcag
    10261 atgcgcgcca acggttaggc tagtatagaa gcgaaggggg aaataggaat gaagaaaacg
    10321 atttttggat ttgcagcgct gatgtctgct gtcgttcttg cagcatgcgg gggcggaggt
    10381 acagatacct ctggaaacaa ctctggaggc aacggcaata acaaagagga attaactttt
    10441 ccaattgcaa caaaaaataa tgatgatgca atcgaagacg gcgaattaga agctgcggtc
    10501 gcaacggaca gtcagtttaa aggattgttc cagtgggagt tttaccaaga ttcatatgat
    10561 gcagctttta tggctccttc tcatgaaccg ctatttgcaa acgatgacga tttcagaatc
    10621 acagacgacg gagctgcaac gcttgatcta gatgaagata cgaaaaaagc gacaatcaaa
    10681 gtaaaagaca acgtcaaatg gtcagatggt gaagatgtaa cggcggacga tgtgattttc
    10741 ccttacgaaa tcattgggaa taaagattac actggtatcc gttatgacga cactttccgt
    10801 aacatcgtag gtatggaaga atatcatgat ggaagtgccg acacaatctc cggaatcaaa
    10861 aaaatagacg acaaaacagt tgaaatcgaa tataaagaag taaacccaag catgcttcaa
    10921 tcgggtggtg gtatctggtc atatgcagca ccaaaacata cgttagaagg tgtagcaatc
    10981 aaagatatgg aatcaagcga tcaagtacgt aaaaatccag tgacttttgg cccttattac
    11041 atgagtaata tcgttgcagg agaatcagta gaatacttgc caaacgagca ctactggaat
    11101 ggtacgccta aattgaaaaa gatcacgatg aaatctgtac cgatggcaag tgcaactgaa
    11161 gcattgaact ctaaattata tgatatgatt ttccaaatgc caacagatac ttacgatacc
    11221 tataaagaaa ttgctggcta tacaaactta ggccgtgaac aaacttctta tacttatcta
    11281 ggattcaagt taggtaaatg gaatgcagat acaaagagtg tggaatacaa tccagattca
    11341 aaaatggctg ataaatcatt acgtcaagca atgggctatg cattagataa taatgcagta
    11401 ggtgaacgtt tctatgcagg tttgagaagc aatgcgactt cattgattcc tccagtattc
    11461 cagtcatacc atgatgatga gatgaaagga ttcacacaag atctagacaa agcgaaaaaa
    11521 ctattagatg atgctggcta caaagatgtg gatgacgatg gtttaagaga agacaaagat
    11581 ggaaataaac taacaatcaa ctttgcttcg atgtccggtg gggaaacagc gcaaccattg
    11641 gcagattact atatccaaca atggaaagaa attggtttgg atgtccaata cacaacaggt
    11701 cgtctgatcg aattcaattc tttctatgat aaattagaaa acgatgatcc tgaaattgat
    11761 gtttaccaag ctgcttggaa tacaggaact gatccaaatc caagtggact atggggacca
    11821 aatgctgcgt tcaactacac tcgttatgaa tcagaagaaa atacaaaatt gatcaacgat
    11881 atcagttcaa gcaaagcatt tgatgaagag taccaaaaag aagcatatca aaaatggcaa
    11941 caatatgcat ttgaagaagc atttgcgatt ccaacgttgt tccgtaatga agtattgcca
    12001 gtcaacgacc gtgtcaaaga ctgggattgg tcttatgatg taccaaaccc atgggcaaat
    12061 gtttctgtta cagcagattc acgttcataa tcactaaaaa tgaccgaaaa agggcgatgt
    12121 cctttttcgg ttttttgata tttttctgtt tatttctcta atattcaggt caaggcgctg
    12181 atttcatgac ttctccaata aacggtgttc aaagtccagt ttctcggtat taagtcaaat
    12241 ttatgcaaaa atatggaaag ccattttcac aaaatttttt cttcatactc gaaactaaac
    12301 gaatttttca caacttctcg tcaatcacta ggattattaa agctttactt accttttgac
    12361 cgaaccattt caaatttgac aaaaaaccag tataataagg aagtatcgaa aaaatatcca
    12421 taaaaggaat gtggaaaatg gacaaccagc tgacaaaaga actaaaagaa aactataaca
    12481 tcgtttttca cgatctaaat ttgcttgaac aagcttttac ccattcatcc tatgtgaatg
    12541 agcatcgcaa tctgcaatta tcggataatg agcgacttga atttttagga gatgccgttt
    12601 tagaactgat ggtttctcag tatttattcc gtttatatcc tcatttgcca gaaggaaaat
    12661 tgacaaaaac acgagctgcg atcgttcgcg aagacagctt gtcgaaattt gccaaagaat
    12721 gtcactttga tcaatatgtt ttgttgggca agggagaaga gaattcagga ggacgtacgc
    12781 gaccagcatt gctttgcgac ctattcgaag cttttttagg cgcgttatat cttgaccagg
    12841 gattcgacac gacccatgca ttcatcgaaa aagtgatttt tcctaaagtc aaagcaggtg
    12901 ctttttcaca tgagatggat cataaaacaa aactacaaga agtcttgcaa aaatcaggtg
    12961 atgtatcaat cgaataccga ttgatcaatg aagaaggtcc agctcatgaa cgagtatttt
    13021 ggatcgaagt ttatgtagat gatcaattga ttggcactgg acaagggaaa tcgaaaaaat
    13081 tagcagaaca agctgcggcg gaaaatgcat tggcagcact gtaaaagtaa gtgagggaaa
    13141 gctttgtatt tgaaacgaat tgaaatagcc ggttttaaat catttgcaga caagacagtc
    13201 attgattttg aaaatagcgt cacagcagtg gttggaccaa atggcagcgg aaaaagcaat
    13261 attacagagg ctatccgctg ggtattagga gaacaatctg ctaaaagtct gcgaggcgga
    13321 aaaatgccgg atatcatctt tgccggttcc gatacccgta agcagctgaa tattgcagaa
    13381 gtcacagtga ttttagacaa tagtgatcat tatctgccat tggagtacaa tgagatcagt
    13441 gtaacccgcc gttatcgacg gactggggaa agcgagtttt tcatcaataa acaaagctgc
    13501 agattaaaag atatccaaga attgttctta gattctggat taggaaaaga atcgttctca
    13561 atcatttctc aaggaaaagt agaagcgatt ttcagtagta aaccggaaga ccgtcgtggg
    13621 atctttgaag aagcagctgg tgtcttgaaa tacaagcagc ggaaaaagaa agcagaacag
    13681 aagctttttg aaacagaaga caacctcagc cgcgtccaag acatcatcca cgaattggaa
    13741 gaacagttaa ctcctttggc cgcccagagt gaggcagcaa aggagttttt acggctaaaa
    13801 gaaacactga ctcaaaccga tgtctctttg atggttgctg aaatcaagac agcaaaaaaa
    13861 gattgggaca acaaacaagc acagcttgcg aaattcaatc tagaattagg caaactatct
    13921 gagtcgatcc aagaacaaga atccattttg gcgaagcagc gtaaagaaaa tgcgcaagcc
    13981 gatcgtctga tcgaaaaaaa tcagcaagta ttattagatc tttcggaaaa actgaagcag
    14041 acagaaggac aaaaagatgt tttgcaggag cggacaaagc atacacaaaa aagctcgcaa
    14101 gaatatcaaa cttcattagc agaagcacaa aaaaaggtta aacattttga aaagcttcaa
    14161 gaaagcttga tgaaagcagc agcagaaaaa gaaacagaga tccaaaaagc tgaagcaaat
    14221 ctcataaaaa cacagcaaga attagaaaaa taccaaaaat caacgaaaga attattggca
    14281 gaattaagag atcaatatgt tgatttgatg caagaacaag cagctgtagg aaacgaactg
    14341 aagtatctag aacgtcaata tatacaagaa acagcaaaaa gcaagcagac gttagccaag
    14401 caatcggaag tagaagcatc agtagaccga ttgatcttac aaaaagaaga attgacacaa
    14461 aagcaggcgc agctaaaatc atctttaact gaaacaaaag agaagttaga aatgatccaa
    14521 caaaacggga aaaagttcca agaagcatta gcaaaagaac agcctaaaat gtatcaatta
    14581 atgaatcaag tccagcagct acgtgctcgt aagaaaagcc ttcaagaaat ccaagaaaac
    14641 tattttggtt tttaccaagg ggttcgattg gtcttgcagc ataaacagca gctttcgggt
    14701 attgtcggag cagtggctga actgatcgat gttcctgcag atttcacgtt agctatcgaa
    14761 gcagctttag gtggggcagc ccagcatgtc atcgttgaaa atgaaaaaga tgctcggcaa
    14821 gccatcacgt atttgaaaca gcaaagagga ggaagagcaa cattccttcc actgacgaca
    14881 atcaaaccaa gacaattacc ggctcatata ttgacacaag cagcagctgt agaagggttt
    14941 atagggattg ccagtgaaca agtcagttat cccgatcaaa tccaaactgt ggttcaaaac
    15001 ctgctaggaa cgatcctttt agccaaagac ttaactagtg ccaatgcaat cgcgcaaacg
    15061 atccgctatc aatatcgagt ggtttcctta gaaggtgatg tgatgaatgc cggaggatcc
    15121 atgacaggtg gagcgaataa acgaggaaac caaggcagtt tgtttgtaca aaatcaagaa
    15181 ttgaaacagt tgactagtga atttgaagaa gcagataaac aactccaagc acaagaaaaa
    15241 aaagttcagg agctgcaaca agaaacagct cgtttagccg aagaacaaga agttttgcgt
    15301 acacgaggcg aacaattacg ttttgaagaa caagaagcga caaatcagct acaaaacatc
    15361 ataaatgaac ttgaacgttt tgaaaaagaa aagcaaatct caacttttga gacacgtgaa
    15421 ctgcagcagt ttatcgaaga ttacgaaaag caaacgaacg aattgaaaga caaacaaaca
    15481 gatcttgaat cgcaacgaca aaaaatcgac gaagaaatca aatcgttaag tcaagaaagc
    15541 gaccagatgg aagccagaag agcacaagta caaagtcaaa aagcacaaga acaagcagat
    15601 cttgctgtct tgaaagagca gtttaatcat ctgcagatcc agctccgcgg tgcccgagtc
    15661 caaaaagcag aggcaaccga acgtcaagaa gcgatagaaa agcagttggc gacattgaca
    15721 gcagactttt ctgatcatga agtcacagaa gaaagtttgg aaaaacaaat taacgaatta
    15781 tctgctcagc gagaaacatt aaaagccgag ttggcaaaag ccaaagagca gcgagataga
    15841 aggcaaaagg agatcgataa gcttgaagca gtcttggcag agcgtaatcg tgagcaaaaa
    15901 gcacgactgt cggaacaatc caaattagaa gtccaaaaag accgagctga aatgatttta
    15961 gataatcatt tgacctatct gcagtcagaa tatcagctga catttgagaa agcttcccaa
    16021 gactatcaag aaacaaccga tatcgaagac agtagaacaa aggtttcatc cttaaaagaa
    16081 cagattgaaa aactgggacc agtaaactta aattcaatcg aacaatacga acaagtcagt
    16141 gaaagacata cattcttagc aacacagcga gatgatctgt tagcagcgaa aaaccaattg
    16201 tttgaaacga tggatgagat ggatgatgaa gtgcggacaa gattcaaaga agtatttgaa
    16261 gctatccgtc aagaattcaa aattgtcttt ccaaatatgt ttggcggcgg taaagctgaa
    16321 ttagtactga cggaaccaac tgatttgctg aaaacaggta ttgagattga agtacaacct
    16381 cctggcaaaa aactgcaaag tctaagtttg ctttctggag gagaacgagc acttacagcc
    16441 atcgcgttgc tcttttccat tatccgtgtc tgtcctgttc cgttttgtat cttagatgag
    16501 gtagaagcag cgttagatga agccaacgtg aaacgttttg gtcgttactt gagcgatttc
    16561 caagatgaca cacaatttat cgtagtcaca caccgcaaag gaacgatggt cgcagcagat
    16621 gtactgtacg gagtgacgat gcaggaatcc ggtgtatcaa aaatcgtttc tgtacggatg
    16681 gaagatatca atgaagaggc gaaaatcaac gaaacgaaag tgtaggaacg aaaatgatca
    16741 aattagtagc aatcgatttg gacggtacat tattaaatga acaaaagctg atttcagatg
    16801 agaataaaca agcattagca caagcaaaac agcaaggggt caagatcgtt ctatgtacag
    16861 gacgcccctt agctgcgatg gcgcattatc tgcaagaatt aggactggta gatgaaggtg
    16921 actacagcat cactttcaac ggcggtttgg tacaaaaaaa tgatactggt gaaatcatgg
    16981 aaaagaaagt gatggaagta tcagatatcc aacgcttata caaactggct cagcaattgg
    17041 atctgccact agatgttttg tctgacaatg tggttttaca gctgcctagt gccccaagga
    17101 agcaatcctt gtacaatgtg ttgaataagc tgctgcaatt ccaaccagcg gatataagcg
    17161 atattacaga tgaatttgtg cttaataagg cagtaatcgc ttatgaccaa gaagaattag
    17221 atcctaaaat caaagaaata ccagcagaat tccatgaaat gtatgagatc atcaaaacaa
    17281 gaagtatgct tttggaattt atgccaaaag gtgttacaaa agcttatggt atttctttat
    17341 tggcaaaaga tcttggtttg aaacaagaag aaatcatggc gatcggtgat gaggaaaatg
    17401 atttaccgat gatccaatat gctggactag gcgtagcgat ggagaatgct gtgcctttcg
    17461 tcaaagaagc agcagatttc gtgacagcca gcaatgaaga aaatggcgta gccaaagtga
    17521 tccaaaaatt tgtattagag tagggggagt caaatgggat tttttgataa aatcaagcgt
    17581 gcttttactg gcgaagagaa aacagaagaa gttgaagtac aggagaagta cgacaagggt
    17641 ttagaaaaga cgcgtaaaac atttggtgaa cggatgaatg agttgtttgc caattttcga
    17701 acagtcgatg aagacttttt tgaagaatta gaagaaacac ttatcggtgc agatgtaggt
    17761 tttgaaacag cgatacgaat caccgaagca ttacgtcaag aagtgaaact cagaaatgcg
    17821 aaaaaatcgg cagaagtcca aaatgcgatc atcgagaaac ttgtggatct ctatgaagcg
    17881 gaaggaatca atgaagtaaa tgaactaaat atccaagaag acggactaac agttattttg
    17941 tttgtcggtg ttaatggtgt agggaaaaca acaagtattg gtaaacttgc ccatcagttc
    18001 aaaaacgaag gtaaaaaagt attgatggca gcagcagata ccttccgcgc tggagcaatc
    18061 gaccagttag ttgtctgggg cgaacgcgct ggtgttgaag ttgttcgtgg caatgcagga
    18121 ggcgatccag cagctgtcgt attcgatgct ttggaaagag caaagacaga gcaggcagat
    18181 gttttactag ttgatactgc aggccgactg caaaataaag tgaacttgat gaacgaatta
    18241 gaaaaaataa aacgagtaat ccagcgggaa ttaccagaag caccccaaga aacacttctt
    18301 gtattagatg caaccactgg tcaaaacgcg atggttcaag caaaacaatt caaagaaaca
    18361 acagatgtga ccggtcttgt tttgacaaaa ttagatggta cagcaaaggg cggtatcgta
    18421 cttgctatcc gtaatgaatt gcatttacca gtgaaattgg tcggtcttgg tgaaggaatc
    18481 gatgacttgg agccgtttga tccaaatgat tttgtcgttg gattgttcaa aggattacta
    18541 aaagaagaat aacccatgtc taaaaagcag gagctgtgac aaaagttgca gctccttgtt
    18601 tgaatgattg tttttaacat agctgtgttt ttgatgcctt catttttata cttatttgta
    18661 agcggttctc aaacaatttc gttctttttc ttagactttt tgattataat tagattcata
    18721 tgaattggag gtcacttaat gatgaatttt aagcaattat tattgcatgt caatgcgcgc
    18781 ccttttatcg accaagctcg atttggtata gaacgggaag gacagcgggt tgatcttgca
    18841 ggaaatctag caaaaaccga tcatccagca atctttggcg atcgatccta tcacccctat
    18901 atccaaacag attttagcga aacacaaaca gagatgatca cccctgttac cgattctatt
    18961 cccgaattat ttcagtatct cgctgctgtt tatgatgtga ctgctcgttc tataccgaaa
    19021 gaagagatga tctggccatt aagtatgcca cctgccttac cggaaaaaga cgaagagatc
    19081 attattgcaa aattaaaaaa tttcgaagat gtcttgtatc gacgttattt agcaaaaaaa
    19141 tacgggaagc gaaaacagat ggtaagcggt attcatttca attttgaatt tggcgatgag
    19201 ttgctaagaa cattgttcag ccatcaggaa gaatttcaag atttttccga attcaaaaca
    19261 gaactttatt tgaaaacagc gagaaacttt atgcgctatc gctggatgat cacttattta
    19321 ttcggtgctt caccaatgag tgagaagaac tactttttag atgaatcaca tccgcaagaa
    19381 cctgttcgca gtattcgcaa cagtgcgctg ggctatacga atcatccaaa tgtgaaagtt
    19441 tcctatgctt ctatgaaaca gtatttagca gatatcgagc gaatgatcga agaagggaaa
    19501 ctttcggagg agaaagaatt ttatacaccg cttcgtttcc gaggcggaaa gaaagtcgca
    19561 gatttagcaa caacaggtgt tcgttatatt gaattgcgga atatcgattt gaatccttat
    19621 gcaagattgg gtatcaatcc agagcaagtt cggttcttac aattattcct gatgtacatg
    19681 ttatggacag aagaaaaaga agactgtgac caatgggtag cagaaggtac gactcgaaat
    19741 aacaaagtag cactagaaca accatcagat caaacagaat ttcatcaaga aggtagagaa
    19801 attcttgaag gaatgaagca aatgctggtt gaattggatt ggttggattc tctttattta
    19861 gtagaggaag cattgactca aatggatcat cctgaacaaa cgttagcagc gaaactctat
    19921 caagaagcac agctatcgag tcagcaagaa gttgctgtgg cattgggaca tcaatattat
    19981 aaagaaagcc atgaacgtcc ctatcagttg gccggttttc gcgaaatgga actttctact
    20041 cagatattta tgttcgatgc catccaaaaa ggtgtccaag tcaaagtatt agatgagtcg
    20101 gatcaatttt tgcgtctgca attccaagac catgtagaat atgtgaaaaa tgcaaacatg
    20161 acaagtaagg acagctatat cgtcccactt atcatggaaa acaaaacagt aacgaaaaaa
    20221 gtcttaaaag aagctgggtt ccgggtacca ggaggcgcag aattttcatc catggaagaa
    20281 gcggtaaaag cttatccgag atttgccgag caggcatttg tgatcaaacc taaatcaacc
    20341 aattacgggt taggtatcac gattttcaag gaaggagcta gtctggaaga ttatcaagca
    20401 ggtttagcca ttgcttttcg tgaagacagt tcggttttag tagaagaatt tatgccggga
    20461 acggaatatc gtttctttgt gatcgacgga gaagtccaag ccatcatgtt gcgagtccct
    20521 gctaatgtta ttggtgacag tatccgaaca gtgaaagaac ttgttgaaga aaaaaatagt
    20581 gaccctttgc gtggaacgaa tcatcgtgca cctttagaat tgatccaatt aggtgaatta
    20641 gaacaattga tgttgaaaga acaagggttg acgatcgaat cagttcccca agccaatcaa
    20701 attgtttatc taagggagaa ctcaaatatc agtacaggtg gtgattcgat cgatatgact
    20761 gatgaatttt ctgaagctta caaaaaaatc gccgtttctg cggtagaagc actaggagct
    20821 aagattagtg gtatcgattt gattatacca gataaagaaa tcgatcctac aactgataaa
    20881 aaagcatatg ggatcatcga agcaaatttc aatccagcca tgcatatgca tgtttatcct
    20941 tttgcaggaa aaggcagaag attgacgatg aatgtattga aattgttata tcctgaagta
    21001 ttttaaaaga aaaagctttt gacagattgt cctatttgat gtcaatctga tcaaaagctt
    21061 tttttgca
//
LOCUS       DADVXU010000049        20742 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.051, whole genome shotgun sequence.
ACCESSION   DADVXU010000049 DADVXU010000000
VERSION     DADVXU010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 20742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.051"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            352..1041
                     /locus_tag="KB953_001634"
     CDS             352..1041
                     /locus_tag="KB953_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBB6866612.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="KB953_001635"
     CDS             1028..2236
                     /locus_tag="KB953_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBB6866613.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="KB953_001636"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="KB953_001636"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBB6866614.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="KB953_001637"
     CDS             complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="KB953_001637"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBB6866615.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6339..7034)
                     /locus_tag="KB953_001638"
     CDS             complement(6339..7034)
                     /locus_tag="KB953_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HBB6866616.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="KB953_001639"
     CDS             complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="KB953_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBB6866617.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8233..8424)
                     /locus_tag="KB953_001640"
     CDS             complement(8233..8424)
                     /locus_tag="KB953_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HBB6866618.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8445..9335)
                     /locus_tag="KB953_001641"
     CDS             complement(8445..9335)
                     /locus_tag="KB953_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBB6866619.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9322..10089)
                     /locus_tag="KB953_001642"
     CDS             complement(9322..10089)
                     /locus_tag="KB953_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBB6866620.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="KB953_001643"
     CDS             complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="KB953_001643"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBB6866621.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="KB953_001644"
     CDS             complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="KB953_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBB6866622.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="KB953_001645"
     CDS             complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="KB953_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBB6866623.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14660..15043
                     /locus_tag="KB953_001646"
     CDS             14660..15043
                     /locus_tag="KB953_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866624.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15211..15969)
                     /locus_tag="KB953_001647"
     CDS             complement(15211..15969)
                     /locus_tag="KB953_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HBB6866625.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15987..16805)
                     /locus_tag="KB953_001648"
     CDS             complement(15987..16805)
                     /locus_tag="KB953_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBB6866626.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="KB953_001649"
     CDS             complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="KB953_001649"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBB6866627.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="KB953_001650"
     CDS             complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="KB953_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBB6866628.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17933..19267
                     /gene="dnaA"
                     /locus_tag="KB953_001651"
     CDS             17933..19267
                     /gene="dnaA"
                     /locus_tag="KB953_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBB6866629.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19465..20595
                     /gene="dnaN"
                     /locus_tag="KB953_001652"
     CDS             19465..20595
                     /gene="dnaN"
                     /locus_tag="KB953_001652"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBB6866630.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
BASE COUNT         5941 a         4225 c         3805 g         6771 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     3841 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     3901 tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag
     3961 agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt
     4021 cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac
     4081 aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag
     4141 gtggcaaggg aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga
     4201 aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg
     4261 aaggagttgc ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
     4321 ttacttttgt cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct
     4381 tttgtgattt gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata
     4441 aattgcgctt catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa
     4501 cgcaaaccgc cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga
     4561 ccttcgattc cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg
     4621 tcttttgaac ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga
     4681 cgtccttgga agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct
     4741 agcatcactg catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc
     4801 cctccgtctg caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata
     4861 gctgtgagct gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt
     4921 ccgataccaa cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc
     4981 gtcgcaacgt ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt
     5041 tttcggatca cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct
     5101 gcttctaata aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct
     5161 acaagcaatc gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg
     5221 tctttgatcg tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg
     5281 attttatgtt tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc
     5341 actaagttgt cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc
     5401 atgtcgcggt ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc
     5461 acaccactga tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgtggc
     5521 gttaagaaga acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca
     5581 tccgcttgct ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc
     5641 atagcaatgg ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata
     5701 ttcaacttaa tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga
     5761 ctttctgctg gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt
     5821 tcccagttgg acatttttag taccactcct ctaatttagt ttatctatta aaataattga
     5881 ttttttcatt taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa
     5941 tataaagcgt ttacatctgt gtgtattaaa agagattgta aaaaagctgt cctgcatcaa
     6001 gtaataagaa cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta
     6061 ccgcagatgc aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt
     6121 tcgagtagta agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt
     6181 aataccgaga aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt
     6241 ttgtcacagt cccttactat tatttaacta tggataaaca aaatgaggtt ctttactacc
     6301 cctagatatc tagcaaaata gctgatcttt ttgctagttt atcttcttgg gacggataaa
     6361 cttccaataa tcccgtattt tttcttcaag tagtaacgga ttgctaaagc agttgccccg
     6421 ataatgataa ctactactgg atctaatact ggattgataa ctggtggcaa tagtgttgtc
     6481 gcagaaaaca gtacgaccca aagaagtgtc gttccaccta ggataagcaa tgtcttaccc
     6541 attcctggtt tcttgcttcg atctgctccg ggatattcat attgatagat gtatttgtac
     6601 atcatataaa agacccagcc accgattgca gaacctagaa cgagtgtaga aaagccataa
     6661 gctggcgttg ttccacgtga aaacaatccg gtaatcgcaa tcatgatact taatagacca
     6721 agcaacagca acgtattatc taaccacatc aataaagggg tggatgaatt ttttacttct
     6781 gctggcttat tgatgattgc ttccgttctt tcagagactg tcccaaacaa ttgacgggct
     6841 gttcttccgc ctttttgttc tgcaactaaa gtaggcagaa tgtcatgaag ggcttttact
     6901 ttttcttctt cagacaaatt cgctgcttct aaagattttt tcaaatcaaa aatgtactgt
     6961 tgatttcttt tggttaattt tgtttctaaa ctattgtttt cttttacgat tgctcgtaat
     7021 tgttctggtt ccattatcta atagtcctcc ttagccgttc cttttctcaa cggtcgtcac
     7081 aaaagacatt ctaccatatt ctacagctag tttgatagcc cttttacaca ttaaagcgga
     7141 ataacattac gtcgccgtct tgtacgacat aatcttttcc ttctaatcga acacgtccag
     7201 cttcttttgc tgcatgcatg tttccgtaat gatccaagtc ttcaaaagag acagtttctg
     7261 cacggataaa tccgcgttcg aaatctgaat ggatgattcc tgctgcttgt ggtgctttga
     7321 ttcctttttt gaatgtccaa gcacgtactt cttgttcccc agcagtaaag taagtagcta
     7381 agcctaacaa atcataagca gcacggatca actgatccaa acctgattct tcgattccta
     7441 aagcttctaa aaattccgct ttgtcttctt catctaattc tgcgatttct tcttctgcac
     7501 gagcactgac cacgatcact tctgcatttt cttctgcagc aaagttgcgc acttcttgta
     7561 cataatgatt cgcgtctgca tctgctacgt catcttctga gacattggct acataaagaa
     7621 ttggttttgt cgtcaataaa aatagtgatt taacgatttt ttgttcttct tcagtaaatt
     7681 cgatcgttcg ggcagatttt ccttcttcca gtacaggttt gattttatcc agtacagcta
     7741 gttctgccac tgcatcttta tctttggttt tagcaacttt tgctacacga gtatagcgtt
     7801 tgttgataga ttctaagtct gctaacacta gttccaagtt gatcgtatca atatcttcta
     7861 gtggatcaac acgtccttct acgtgagtaa tattatcatc atcaaaacag cgaacaacgt
     7921 gacaaatagc gtctacttgg cggatatggc ttaagaattg atttcccaaa ccttctcctt
     7981 tacttgcgcc ttttacgatt ccagcaatat ctgtaaattc aaaagtcgtc ggaacagttt
     8041 ttttcggatg tacaagttct gttaagcgtt gcaaacgcca gtcaggtact tctaccatcc
     8101 ctacgttggg gtcgatcgtc gcaaagggat agtttgctgc ttctgctcct gcttttgtga
     8161 tcgcattaaa caaagttgat tttcctacgt tgggcaaccc aacaattcca gcagtcaatg
     8221 ccataaattt attcactctt tctctgcatt tttttcaatt acttttttta atttcttttc
     8281 aaaatctcga cgggtcatca tgacgatatg tccgcaattc atacatctga ttttgatatc
     8341 tgcgcccatt cgaatgactt cccagcgatt cgtctggcaa gcgtgaggtt ttttcatttc
     8401 aacgatatcg tgcaagtcgt acataaaaat agcctccttt ttgtttattc ttcatcaaat
     8461 tgaatattca agatatcaag aattcgagtc aaatcttttt gagaaagata ttcaatctcg
     8521 atcttacctt tcccattttt ttcttggata gctacacttg taccaaattt atccatcaaa
     8581 cgatcttcgc tttctcggat ataatatggt ttttcctttg ctagccttgg gatttttttc
     8641 ttttcttttc catcattttg atttaactca gaaacaagct gttccagttg gcggacagtt
     8701 aaattttcag ttactgcacg attggctaat ttcaagatca atgttttatt ttttaaacct
     8761 aacagcgtac gggcttgtcc catagataaa cgctgatctt gtaccatttc cttgacaaga
     8821 tccggtaaag aaagcaagcg caaataattg gcgatataag gtctgctctt tcctaaacga
     8881 ctagctactt ctgcttgtgt tagtttcagg tttttcatca gcatctcata tgcttctgct
     8941 tcttctaacg gattcagatc ttctcgctgc aagttttcca gcactgctac ttgcatcatc
     9001 gcttcttcgt caaagtctct aacgatagca gggatcgttt ctttttcagc tagtttggac
     9061 gctcgaaaac gtcgctcccc tgcaataatc tcatatcctt ttactgcgga acggcgtacg
     9121 atgatgggtt gaaagacacc tgattgttca atagaattag ctagttcctg caaagatact
     9181 tcttcaaatg ttttacgggg ctgatacgga tttggtcgca gctcattcaa tggtagctgg
     9241 acaacggtat catcttttac atccaccgtc tctaagtttt ctaaatcttg aaacagtgca
     9301 tcgattcctc ggcctaatcc tttacttttc ttcacgactt accacttcct ttgccaatgc
     9361 ttggtagact tcagctcctc gagaacgtgg atcgtagtcg atgatcggtt ttccatgact
     9421 aggtgcttct gataaacgaa tatttcttgg aatgatcgtt tcgtaaactt tttcgcggaa
     9481 gtatttacgt acttcttcta cgacttcatt tcccagattt gttcgtgcat catacattgt
     9541 cagtaagact ccctcgatct ctaattctgg attaaagtgt ttttggacta aacgaaccgt
     9601 attcagtaat tggcttaatc cttccaatgc gtaatactca cactgaacag gtatcaaaat
     9661 cgaatcactt gctgtaaatg aattgattgt cagatggcca agagaaggcg ggcaatcgat
     9721 caaaatataa tcatactgac tgcttacttc tgctaaagat ccttttaatc ttgattctct
     9781 tgccatcatt gacgtcaatt cgatctctgc tcctgctaat tggagagtcg caggcacaat
     9841 actcaaattt tcgtgttcag tgatcaaagt tgcctcatca attggcaatt cattgactag
     9901 aacatcatag atgtctcttg tcacatcagg ttttctgatc ccgacaccac tggtagcatt
     9961 tccttgtgca tccatatcca ctagcaatac ttttttgcca aggctagcaa gacaagcacc
    10021 tagattcacc gtcgtcgttg ttttgccgac gccacctttt tggttcgcaa cagatattat
    10081 ttgtgccatc tcttctcctc ctatttgatt ggctgcttat taggcagtcc gggctttctt
    10141 gggtatttct ttggggttgg tttctttttt tgaataacga tgatatgccg ttgatcagag
    10201 acattcggca aggtaaccgc ctcttctttg aggaatttac cacctaataa agcaatcgct
    10261 tgttttgctt ctgtcaattc ctcttcactt tttgctgctt tcaatgcata aaaatagcct
    10321 tcttgtttag ccaagggcaa acacaattcc gctaacacat tcaaacgagc aaccgcacga
    10381 gcagtgacaa ggtcaaatga tgcacgaaag tgaggatttt gcccaaatgt ttctgcccgg
    10441 tcatgatata aggaagcatc gattcctaat aatcgggtta actcagataa gaaggttatt
    10501 cgtttattca gagaatcaac gatcgttatc tgcagttctg gaaaggcaat ttttaaaggt
    10561 atgctaggaa accctgcccc cgaaccaaca tcgcataaag ataaaggttg cgtaaagtct
    10621 acttcaaatg ctaatgtaag tgaatcgtaa aaatgcttca gatatacgtc tttttcatcc
    10681 gtaatagctg taaggttcat cttttcgttc cattcgacta atgtccgata ataaatagcg
    10741 aattgattca tttgatgatc ggtaagctct atcccttttt ctgccaagga ttggcgaaat
    10801 tcttctggta tcataaatat tcctttcatt gtgaaacagc aattgtttca cagattctct
    10861 ccctacttta acgcaaaatc tctagacatg caatccttcc atatggattc ttaaaaattt
    10921 gaaaagtaag aaaagccgat agactttttc tttctagtat gactcataaa atatacacag
    10981 aagagagaac tagctttgag caatttctgc aaaaactagt tctctcttct atttattatt
    11041 acgtattacg ctggttcact gactttcgca atcttccctt gttcgatata gaccattagg
    11101 atacttaggt ctgcagggtt tacgccactg attcggctag cttgggcaat tgtttctggt
    11161 tggattttct gcaatttttg ttttgcttct gttgctaatc cgttgattgc tgcgtagtcg
    11221 atgttttctg gaatcttttt cgcttccatg cgttttaatt tttctacttt ttccatggct
    11281 tttttgatgt agccttcata tttgatttgg atttctactt gttcgatttc ttttcgtgtc
    11341 aattcttcat tttctggaat aaaccgtaga agttctgcat aagtaatctc aggacgtctt
    11401 aaaaagtcgc ttgctagtac accatctttt aatggtgccg cattaatcgt tgctaaaaag
    11461 tcttggactt cttctgttgg tttgattcgt acattcatca aacgtttgat ttcttgttca
    11521 actgcttctt ttttcttcaa ataattcttg tattgatctt cttttactaa gcctacacgg
    11581 tgacccattt ctgttaatcg caaatctgca ttatcatgac gtaaaagcag acggtattca
    11641 gcacgtgaag tcagcaaacg ataaggttcg ttcgttcctt ttgttaccaa atcatcgatc
    11701 atgacaccga tgtaaccgtc acttcgtttg atcactaaag gttcttttcc ctgaactttc
    11761 aaagctgcat tgataccggc aatcagccct tgtccagccg cttcttcata accgcttgta
    11821 ccatttgttt gtccagctgt ataaagattt tcaacgactt tagtttctaa tgtcggacgg
    11881 agctggtgtg gctctactac gtcatattcg atagcatatc ctgtacgcat catttctact
    11941 ttttctaatc cagcaataga atgaagcatg ttgacttgaa catcctctgg taaagaagta
    12001 gataaacctt gaacatagac ttcttccgta tttaagcctt ctggttccaa aaataattga
    12061 tgacgtggtt tgtctgcaaa acgcacgatc ttatcttcga tggatggaca atagcgagcg
    12121 cctacccctt caacgatccc tgtaaacatt ggggcacgat gtaagttgtc tcgaataatt
    12181 tggtgagtcg tctcacctgt ataggtcagc cagcaagatt cctgatctag tttataggca
    12241 ctatctggtg tgctgaagct aaaatgattc ggctctttgt caccaggctg ctcttctgta
    12301 acagaatagt cgatcgtact tgattttact cgaggcggtg ttcctgtttt gaaccgttcg
    12361 atagcaaagc caagttcttt tagatgattc gctagtccaa tagaaggttg agagttattc
    12421 ggacctgatg aatatttcaa ttcaccgata atgatttctc cgcgaagtgc tgttccggct
    12481 gtaataacaa ctgctttggc ccggtagctc gctcctgtgg acgtcactac tccatgacaa
    12541 acaccatctt cgacgatcag ttcttcgacg attccttgtc ttagcgtcaa atttggttct
    12601 ttttcgatcg tatgtttcat ttctgttgaa taagcatgtt tgtctgcttg tgctcgcaat
    12661 gctcggacag ctgggccttt tcctgtgttc aacatacgca tttggatata ggttttatcg
    12721 atattcttac ccatttctcc gcctaacgca tcgatttctc gaacgacaac gcctttagct
    12781 ggtccgccta cagaaggatt acatggcatg aatgctacca tgtccaaatt tattgtcagt
    12841 aataatgttt ttgatcccat acgtgcagca gcaagtgcag cttctgaacc ggcgtgtcct
    12901 gcgccaacaa cgatcacgtc atacgcatct gcttcataat gttccaatcg tttgttcctc
    12961 cttttatttt cctaaacaaa attgactgaa taattgagtg attagctcat cttgtacact
    13021 gtcgccgaca acttctccta gatagtccca gcaacgcgtc atatctattt gaacgagatc
    13081 cacaggcatt cctgaatcaa tacctgcaat gacttcttca agtgagaggg aagctttttc
    13141 taacaacgcg atatggcggg tattagaaac ataagtagcg tctctttctc ctgtttctcc
    13201 gctgaagaat aaatcagaaa tagctgattc caaacgatct agtccgtcgt ttttagctac
    13261 ggaaattgaa aaaactggtt cattttcgat caatttcttc aatttttctt gttccagctg
    13321 tgctggcaaa tctgttttat ttagaagaat gatccgtttc aatccacttg tcgcttccaa
    13381 taattgttca tcttctgctg tcagtggttc gctttggttt agaacaagta agatcaagtc
    13441 tgcttctgct aatgcttttc ggcttctttc gaccccgatc cgttccacca catcttctgt
    13501 ttcccgaatt ccagctgtat cgattagctt gagcggtacc ccccgaacat tgacatactc
    13561 ttcgattaca tctcgagtcg ttcctgcaat atccgtaacg attgcttttt cttctcttaa
    13621 caaatggttt aacaaacttg atttgccgac atttggccgg ccgatgattg ccgtacttaa
    13681 cccttcccgc agaaccttcc cttgcttgga tgtagcaagt agcgcttgga ttctttgttg
    13741 gatcatctgc gctttttcaa gcaataactt cgtagtcaat tcttcgacat catcatattc
    13801 tggataatca atatttactt ctacttgtgc taatgtctct aaaatctctt gtctcaatga
    13861 gcggatcaaa gcagacagat tgccgtctaa ctgattaaga gctagtccca ttgctttgtc
    13921 tgtttttgcc cgaatcaaat ccatcacagc ttctgcctgt gaaaggtcaa cacgaccatt
    13981 caagaatgct cgttttgtga attcgcctgg ctcagctaat cttgcacctt ctcttaacaa
    14041 tagctgcaaa atttgattga ctacaacgat cccaccgtga caattgattt ctactacatc
    14101 ttcccgcgta aatgtctttg gtgctctcat cacggagacc atgacttcat cgacaagctg
    14161 attgctcttg ggatcaacga tatgtccata atggatcgta tgacttggta cttctgataa
    14221 tcgtttgttt ccagattgat aaactttatc tgctagttgc acagcttgat cgccacttaa
    14281 acgaacgata ctgattgcac cttctccagg tggggtagaa attgccgcaa tcgtatcaaa
    14341 ttctaaagta atttcagcca ttttttcact tcctttttca ttttgcgcac aaaaaaagtg
    14401 cccactccac cctttatgca caataaacgg tgaacttcgc actttgactg cttgttcttg
    14461 attaaattct ggtcatagat tatcacactt gctataagga atcaagtgtt taaaacaatt
    14521 agatgaagtt gagcctgttc tgcttccatt accttatatt taattgtcct tggatgtctc
    14581 tttgcgttct ttggtttatt cgaagagaag atactgtata aacaaggctt tatctttctt
    14641 aaaaaaaacg gtacactaaa tggtagcgct atctaaacta tacacttttg aaaggagaaa
    14701 atatgtgagc ccgttcagat ctgctgcatt ggaagaagaa agtgaccctc ggatcaacga
    14761 tgtcgtttta tctacaggga atatcaacca gaaatatatc gtccgcgata tcgtttttgt
    14821 tgtggaatgc ttagaagctg atttgtttga cccatctttt cccaaagatg accttttctt
    14881 aacaacgaag aggaaattaa agacgaaggt acttgaatat ggtgcgaatg cagtaatcaa
    14941 ctgtcatttt gagtatcagc aaaaaaaacg cgacggaaag tattttctgg aactttttgc
    15001 ttatggaaca gtgatccaat ttatccaaac aacgattggt taaatgaaac agacaaaaaa
    15061 agcggatcaa aactgaagac agtttcgatc cgcttttttc tatcaaagtt agtgactact
    15121 tcatcgtata ctctgataag aatgatttct agtaaaatat acttggtttg aaaaactttg
    15181 tcaacgtgat ttattcaaaa agtaatggaa tcaataatat tttttagcag gttctactac
    15241 taaataacga taaggttcct caccttccga atgtgttttt acatgttcat ctttgcttaa
    15301 tgcagcatgg atttgtttac gttcaaatgc aggcattggt tctaagaaaa caggctgccc
    15361 tgtccgtttt actttttcag ccgtgcgatc agctaaacgc tgaatgatct cttggcgctt
    15421 ttcacggtaa ttgcctacat tgactactac agacagctta tttcttgcta aacgatggat
    15481 gaagacttgt gccagatatt gcaatgcatt cagtgttttt ccatgtttcc caatcagtat
    15541 gccttgcttt tgtgtttcta aatggaaaac aaccatatta tttgattctc tttccatttt
    15601 gacaagagct ggagcatcaa gctgttttga aatttcagta agataaactg ccaattccgt
    15661 taaagctgct tcatcaccta gctcacttac ttcttctgtt ttttcttcaa gaatcgtttc
    15721 gttgactacc tcgatctcag ctgttggttc ttcttccaaa atttcttcta cagtctgttc
    15781 tactgtttca gaaacttttt cgctgatact tggctggatc gaaagccgtg cattctttcg
    15841 acccattcct aggaaacctt tttttccttc ttccagtatt tcgatttcga cttggtcttt
    15901 ggccaaaccc aaatcagcca gacctagttg gacagcttcg tctattgttg cagcttcata
    15961 aatcggcatt agcattacct cccctattat ttccgttttt tcttaggatt cattgctttc
    16021 ttaagagcac gttctctttc gcggattctc cgtgcttctt cttcacgctc ttttcgaatt
    16081 ttgaaagggt tgttgatcag cagcgtttgt acaacttgga aggcattgga aactacccag
    16141 tataatgaaa gaccgctggc taaattgaca cccatcacta aaatcattac aggcatcacg
    16201 aaattcatga tcttcattga tgcattcgat tcaatttgac tcatgctaga taaatacgta
    16261 cttgcaaaag taaagattgc tgctaaaatc ggcaaaataa atgttggatc cgcgttccct
    16321 aagttcaacc ataagaaatg cccttgtttc aactctggta cacgtgaaat agattgccat
    16381 aacgccatca agattggcat ttgtactaat aaaggaagac aaccagcata tggattgacg
    16441 ccattttctg catataaacg ctgctgctct tctcttaact tactttgtgt ttccggatct
    16501 cttgaactat attgttgctg cagtgctttt aatttcggtt gtaattcttg cgttttacgc
    16561 atgctctttg tttggaaatg catcaacggc aataggatca cgcggatgat cagtgtaaat
    16621 aaaatgatcc ctatccctgc attaccaaat gacaacgcct tgatcgcctc agcaaagtaa
    16681 taaacaatat aacgatccca aattcctgta ctctgtgcgc tgacttcccc agttccgcaa
    16741 ccggataaga cgatgaccaa actaacgata ccggccatca aaagtaaacg tttgtatttc
    16801 ttcacttact cttttcctct ctgtcgatta tttttgcaag ttttaagacg tggactaaat
    16861 tggagcgaac ctcatcataa gtcaacccct tcacacttgg tctagcaatc acaataaaat
    16921 ccaactccgg atcaatttct tcttttatag aataaactgc tgctcggatt tttcgtttga
    16981 cttcattgcg gtttaccgcg ttgccgacct tttttcctac agataaacca actcgaaaat
    17041 gaggctgtcc cggtttttcc aaacggtaaa cgacgaattt gcggttagca aaagaatctc
    17101 ccgtatggaa cacggcttga aactctcgtt ctttttttac tcgatacgct tttctcatta
    17161 gttttctttc cttttctatc tctcacttac gctcataatc ataccataat tacgattgtc
    17221 ctttcatcat ttccctaaaa aagagtataa aaaaaaccac tgaaacccca gtggcctaag
    17281 ctgaaagaac ttttcttcct ttgcgacgac ggctagctaa cacacgacgt ccatttttag
    17341 tactcatacg tttacggaat ccgtgaactt tttgacgttt acgtttattt ggttggtatg
    17401 ttcttttcat atattccacc tccaaaatgt tcttatctat tttataaaca cagacatact
    17461 ttgatagtat aacgacaaag tagtgatttt gtcaatataa aatagaagat cgattgttaa
    17521 aacttatcca caagattatt tttttctatg ggaatcctat gtggaaaaag ttatgcacag
    17581 ggattatcca cagataataa aaggctggtt ttacacagaa aacacacgtg taaaaaaagt
    17641 aatccacaat caggcgattt cctgtgtatt ccgtctccta actattggta tatctgtatt
    17701 ttttatccac agacgcaaga ggaaatctat tggtttttac gagttttcca cctgtgcatt
    17761 agtggtgtgg aaaacttttt ttgacaactg tgtttttctc ttttttcttt ttgtcgattg
    17821 tgtggataat tgtaattaaa aaattttttt cttcggtttc tgtggaaaac tattgaaaaa
    17881 aatgctaaat tagtactagt tattcacttt ttaatgaagg aggaggcctt atatggtatc
    17941 cctcgatgct ttatggaatg aattaaaagc aacataccaa aaggatttat cgcctgctag
    18001 ctataataca tggatcgaaa cagcgaaccc tcgaaccctt gatcaaaacc agctcgttgt
    18061 tgaggttcct agcaagattc ataaagaata ttgggaaaaa aacctggcga caaaaatcgt
    18121 tgaagtcggt tatatgttat caggaaatga gattatccct cgctttatta ctggcgaaga
    18181 agcagagcaa gaagaagtta tagaagaaaa aaatccaaaa gttgtggcac ctagtccgct
    18241 aaaaaaagcc atgctgaacc ctaaatatac ctttgatacg tttgtcattg gtaaaggaaa
    18301 ccagatggct catgcagcag cgctcgttgt agcagaagat ccaggttcta tttataaccc
    18361 gctcttcttt tatggaggcg tgggacttgg taagactcac ttgatgcatg caattggtca
    18421 tcaaatgctg caaagtcagc ctaatgccaa agtaaaatat gtaagcagtg aaacttttgc
    18481 taatgatttc atcaattcga tccaaaacaa aacagcagaa gagttccgtc aagaatatcg
    18541 aaatgttgat ttactgctag tggatgacat tcaatttttt gcagaaaaag aagctacgca
    18601 agaagagttt ttccatactt tcaatgcctt gtacaatgaa gggaaacaaa tcgtactaac
    18661 cagtgatcgg ttgccaaacg agattccgaa actgcaagaa cgccttgttt ctcgctttgc
    18721 atgggggctg tctgttgata ttacaccgcc ggatcttgaa acaagaacag cgattttacg
    18781 taaaaaagcg gcggctgaac gtctagagat cccagatgac acactaagct acatcgcagg
    18841 acaaattgat tctaacattc gtgaattaga aggtgcgctt gttcgagtgc aagcttttgc
    18901 tgcgatgaat agtgaagata tttcgactag tctagctgca gacgcactga aaacgttaaa
    18961 atcaggaaaa ggacatcctc aattgtcaat cctgcagatc caagaagaag tggcaaagta
    19021 ttatcacatc caattgaaag atctcaaagg aaagaaacgc gtcaaatcta ttgttgttcc
    19081 tagacaaatc gctatgtatt tggcacgtga gctcacagat aattctttac cgaaaatcgg
    19141 agctgaattc ggtggcaagg atcatacaac ggttatccat gctcatgaaa aaatccagca
    19201 gctgatggat tctagtgtca gtatgcaaaa tgaagtatca gaaatcaaaa actttttact
    19261 aaattaattt gtcgattcta tcaactgaaa atagcatgtg gataaaagaa aaaaacgttc
    19321 aaaagttata cacacttttt ccacagctgt tttttcgttt ctctcttacg ttttttgagt
    19381 tttccacaga atcaacatgc cctattactt ttattattct ttttaactaa taataaataa
    19441 acaaacattc aaaggagata catcatgaaa gttactttaa accgagctag ctttatgcag
    19501 gaattgcaaa ctgttcaacg agctatttca agcaaaacca cgatccctat tttgacaggt
    19561 gtaaaaatca cactgacaca agaaggtttg actttgacgg ggagcaacgc tgatatatca
    19621 attgaaactt ttttgtctgt tgaaaacgaa aaagcaaata tgcaaatcga atctactggt
    19681 tccattgttt tacaagcacg tttctttagc gaaatcattc ggagacttcc tgaagaaaca
    19741 tttactttag aagttttaga aaataaacaa gtagcgatca cttctggaaa agcgaatttt
    19801 atcgtaaatg gattagatgc agataactat cctcatcttc ctgttgtcga aagccataac
    19861 cagatgaaat tacctgtaca cgtattgact aaactaatca acgaaacagt ttttgctgtc
    19921 tcacaacatg agagtcgtcc aatcttgaca ggtgtgcatt tcattttatc tgataattct
    19981 ttattagctg tagctactga ttctcaccgt ctaagtcaac gcgtgattcc agtagaacaa
    20041 gcggctgatc attttgatat tgttattcct ggaaaaagtt tgatcgaatt atctcgctca
    20101 ttaacaaatg aagaagaaat cgtcgaaatc agcattatgg aaaaccaagt gttgttcaaa
    20161 acagaaacga tgtatttcta ttcccgtttg ctagaaggaa actatccaga taccaatcgt
    20221 ttgattcctt caagttttaa tacagaggtt gaattttctg ttcctagctt tttagcggcg
    20281 atcgaacggg cttctttact ttctcatgaa ggccgtaaca acatcgttcg tttatctatt
    20341 cgtccagatg cggttgtttt atacggaaat tcgccagaaa tcgggaaagt agaagaaagc
    20401 ttgagttata cagcaagcag tggcgatcca ttagatattt ctttcaatcc ggattatatg
    20461 aaagcagcgt tgcgtgcatt tggagatatg agcatcaaag tgaaatttat ctctgctatt
    20521 cgtccattta cattggaacc aacggaagat ggcgttcaat ttattcagct gattactcct
    20581 gtacgtacaa attaacaatg attttcaatg ttcttgaaaa tttaattgaa aagaaataga
    20641 aaaaataaaa ttttaaacag aaggccctaa aatctctttt aaggctttct gtttattttt
    20701 gtttaaatca ccttttttag attaaatcgt cttatatgga aa
//
LOCUS       DADVXU010000050        19633 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.052, whole genome shotgun sequence.
ACCESSION   DADVXU010000050 DADVXU010000000
VERSION     DADVXU010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.052"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="KB953_001653"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="KB953_001653"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HBB6866631.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="KB953_001654"
     CDS             complement(1172..1867)
                     /locus_tag="KB953_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HBB6866632.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="KB953_001655"
     CDS             complement(1880..2596)
                     /locus_tag="KB953_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HBB6866633.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="KB953_001656"
     CDS             2926..3534
                     /locus_tag="KB953_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HBB6866634.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="KB953_001657"
     CDS             3859..4182
                     /locus_tag="KB953_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HBB6866635.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="KB953_001658"
     CDS             4364..5734
                     /locus_tag="KB953_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBB6866636.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="KB953_001659"
     CDS             complement(5864..7165)
                     /locus_tag="KB953_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBB6866637.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9477)
                     /locus_tag="KB953_001660"
     CDS             complement(7519..9477)
                     /locus_tag="KB953_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HBB6866638.1"
                     /translation="MAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="KB953_001661"
     CDS             complement(9654..9920)
                     /locus_tag="KB953_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBB6866639.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="KB953_001662"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="KB953_001662"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBB6866640.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="KB953_001663"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="KB953_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBB6866641.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /locus_tag="KB953_001664"
     CDS             13264..13827
                     /locus_tag="KB953_001664"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBB6866642.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="KB953_001665"
     CDS             complement(14080..14601)
                     /locus_tag="KB953_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HBB6866643.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="KB953_001666"
     CDS             complement(14604..15446)
                     /locus_tag="KB953_001666"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HBB6866644.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="KB953_001667"
     CDS             complement(15439..16164)
                     /locus_tag="KB953_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBB6866645.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="KB953_001668"
     CDS             16340..16636
                     /locus_tag="KB953_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866646.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17204..18796
                     /locus_tag="KB953_001669"
     CDS             17204..18796
                     /locus_tag="KB953_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBB6866647.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="KB953_001670"
     CDS             18809..19549
                     /locus_tag="KB953_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBB6866648.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         5619 a         4028 c         3725 g         6261 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagccattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaagtaaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgagatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttct gaa
//
LOCUS       DADVXU010000051        19085 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.053, whole genome shotgun sequence.
ACCESSION   DADVXU010000051 DADVXU010000000
VERSION     DADVXU010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.053"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(387..1853)
                     /locus_tag="KB953_001671"
     CDS             complement(387..1853)
                     /locus_tag="KB953_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBB6866649.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(2144..4228)
                     /gene="cadA"
                     /locus_tag="KB953_001672"
     CDS             complement(2144..4228)
                     /gene="cadA"
                     /locus_tag="KB953_001672"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBB6866650.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
     gene            complement(4753..5112)
                     /locus_tag="KB953_001673"
     CDS             complement(4753..5112)
                     /locus_tag="KB953_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HBB6866651.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(5142..5483)
                     /locus_tag="KB953_001674"
     CDS             complement(5142..5483)
                     /locus_tag="KB953_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866652.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(5480..6154)
                     /locus_tag="KB953_001675"
     CDS             complement(5480..6154)
                     /locus_tag="KB953_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBB6866653.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(7330..8628)
                     /gene="asnS"
                     /locus_tag="KB953_001676"
     CDS             complement(7330..8628)
                     /gene="asnS"
                     /locus_tag="KB953_001676"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBB6866654.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(8668..9858)
                     /locus_tag="KB953_001677"
     CDS             complement(8668..9858)
                     /locus_tag="KB953_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBB6866655.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(9879..10406)
                     /locus_tag="KB953_001678"
     CDS             complement(9879..10406)
                     /locus_tag="KB953_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HBB6866656.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(10453..13242)
                     /locus_tag="KB953_001679"
     CDS             complement(10453..13242)
                     /locus_tag="KB953_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBB6866657.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(13389..13589)
                     /locus_tag="KB953_001680"
     CDS             complement(13389..13589)
                     /locus_tag="KB953_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBB6866658.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(14042..14362)
                     /locus_tag="KB953_001681"
     CDS             complement(14042..14362)
                     /locus_tag="KB953_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866659.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(14407..14577)
                     /locus_tag="KB953_001682"
     CDS             complement(14407..14577)
                     /locus_tag="KB953_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866660.1"
                     /translation="MTKIISQIFVKFRLISEEATSGTPFKKEVVTWSRPLFDDYDTKN
                     IIYEQNVNNGRS"
     gene            complement(14865..15983)
                     /locus_tag="KB953_001683"
     CDS             complement(14865..15983)
                     /locus_tag="KB953_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866661.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            16454..17761
                     /locus_tag="KB953_001684"
     CDS             16454..17761
                     /locus_tag="KB953_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBB6866662.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            17882..19081
                     /locus_tag="KB953_001685"
     CDS             17882..19081
                     /locus_tag="KB953_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBB6866663.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
BASE COUNT         5469 a         4026 c         3323 g         6267 t
ORIGIN      
        1 aataaatggt gttcaatcag ctgaccttca gcattagctc aaaaagcggc aaaatatgag
       61 gagctatttt tccgtttgtt tctctaatac tcaggtcaaa tcgctgattt tacaacctct
      121 tccttaaacg gtgttccaaa gcttgcatct gcggtaataa gcccagccaa acgaaaaata
      181 tgaggggcta tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac
      241 ctctgattca cggtgttcca gaagtagctt cttcgaaaaa caagccgaaa cttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttgt aacaagtatt tctattttaa ggctgaacca ctgtttctgg ttcagtgatt
      421 tctttgtttt tgtctcgacg agcaattgct ggcaagatca tacctaataa gatcaagacg
      481 attggtgtga tgatattcgt aaataattgg aaccaccatg ctttaggatc agcagtataa
      541 tctacttttg gcaccattcc taggatacat gcaaacgcag tgaaggcaaa gcaccaagca
      601 ccaaagataa aaccgatttt tggattttta acaaatttat attcagaatt gaatttctta
      661 taagctttat tcaacatcat atatgccaag aacacccata ggtaacgcat tggcatgaca
      721 actgagttca agttcgtcaa ccattttact aattcattca tatctttgat accaaaaatc
      781 ggcaacaaaa taatgattcc tactagtata cctgtcagaa gataaccatt caccaatgta
      841 cctttacgat tacgtttacg tagccagttt ggtacaaatt ctggatccgc atctgctaac
      901 aagattttta gtggtgcatc aattgaaaag gctaatgcgg agatttgtcc aatcatgttt
      961 gtcaatgcgt aaatgatcat caatgagtta ccgataccat agtaattacc taaaatttga
     1021 aaagcactgt atgccccatt tgccatcaag tctttaggaa tattatcact agcaaacagc
     1081 atacccattg cgactgagcc taatacagca caaatcccta ccattgctgc taagaagatc
     1141 atccctttag gaaattcctt tgcaggattt cttgtagagt tgacataagg ggatattttt
     1201 tccgcccctc caactgcaaa tactagcatc gaaacggttg tgaaataact gaaatcaaat
     1261 gttggaatat atgttttcac ttgattcata tgtggtgtgg caaattccat cgttggattg
     1321 atgaacggtg ccgttacagc taaaagaata aataagatcg acatgacgaa catcgctgtt
     1381 ccggccagtc cgccaatcac tttcaaagta gaaagtcctt ttgttgataa atataaaaag
     1441 gcaagaaata taataagtga aataactgct accatttgta ctgacatcgt atttacgata
     1501 tttccatttc cttggactgc ccaaccacca gcaatcagaa ttgcttgagg cttttgtgcc
     1561 aaataaggaa tatggacgac ccagtaagtc caagcggcat agtatgccag tcgtttatta
     1621 gatgtcgtat tttcgaccca tgaactgacg cctcctttac tatccttgaa ggctgatcct
     1681 aattgtccaa cgatcaatgc gtatggaata aaataaatgg ccaaaatcaa tagccaagag
     1741 gtgacaactg atactccttg ctgagcatag ttattaacta cattccccat gccccaaacc
     1801 atgttaaatg caattaacgc aacagtaaac cagcgtaatt gtttgttgtt cattcgttct
     1861 ctcctttatg ataaaaattt gttatccaga tgtaatcata ttcaattttt aacaaactta
     1921 caagacagtg ttattttatg tgtgtattct tttatttaat acacatcgtt ttttcatatt
     1981 ttttccatca aaaaaataag ttattttctt ttatttcatt gaccttattg aatttcaagc
     2041 tattttgaat tttctgcaaa attaaaaaca ttagttattt tataatccta tctattttct
     2101 ttacattttt cattaagaaa aggatataaa aaaaagagga cgttcattcg tcctcttttt
     2161 ttacatgcaa gcttcgcatg gcattcaaaa cagctaatac tgtcacccca acatctgcaa
     2221 atactgcatc tcccattgat gcaaaaccaa aagcacctaa ggcaagtacc aaaattttta
     2281 tcccaatagc aaagacaatg ttttgtttga cgattttcaa tgttttcttt gctaaatgga
     2341 ttgcttctgc aatcttcgat ggttgatcat tcatgatgac tacatctgct gcttcaattg
     2401 cagcatcgct tcccaatcca cccattgcga ttcctacatc tgctcttgct agcaccggtg
     2461 catcgttgat tccatctccg acaaatgcgg tctttttatt gtttctttgt aagatttcct
     2521 ctaaccgctg cactttatct tcaggaagca attcgctata tatcttgtcc acgcctactt
     2581 gcttcccgat atgatcagca atttttttcg agtcgcccgt caacatgact gtgttcttca
     2641 caccagcctg ttttaattga accaatgcgt ctacggcatc ttctttcaag gtatctgcaa
     2701 taacaagata accactgtaa ctttgatcga tcgctagata aagcaatgta ccgatttctt
     2761 gacttaaagg aaaagagatt tcaaatcttt ccatcaattt gtgattacct gctaatactt
     2821 gtttgccatc aacttcagct aagatccctt cacctgcaaa ttcctggata ttagttgttg
     2881 gtaaaagcgg acctgcgtag ccttctaaaa cagattgagc aatcggatgt gtagaaaact
     2941 gttctgcact cgcaatatat tgcatgaatt tctttggatc gatttttgta tcgatcgtct
     3001 ggatcgaaaa gtcacctttc gttaatgtgc cggttttatc gaataccact gtctcggttt
     3061 gcgcaagcaa ctccaagtaa tttgaacctt ttactaatac accgatttta cttgcgcctc
     3121 cgataccgcc aaagaaacta agtggcacgg aaatcactaa tgcacatgga catgagatca
     3181 ctaaaaatgt caatgctcga tagatccatt cagaaaacgc cgctccagaa acaacaagtg
     3241 gaggtaaaac agctaataaa acggccaatc ctacaacgac tggtgtgtaa tagcgcgcga
     3301 atttagtaat gaaattttca gctggtgctt ttttactgct ggcattttct actaattcta
     3361 ataatttgct gatagtagaa ttttcataag atttttctac ttgaactaaa aatggtttat
     3421 ttttattgat aaatccgctt aatactgttt caccaacata tacagattta gggactgatt
     3481 ctcctgtaag tgctgacgta tcgactaatg attctccttc gatgatcgtt ccatctaacg
     3541 gtacacgttc accaggcttc acaagtacaa actgaccgat ctgcacgtct tcagggttca
     3601 cggtcatcaa tccttccgtc gtctgcacgt gagctgtttc tggacggatc gccatcaatt
     3661 cgcgaatcga ttttcttgaa tgactgactg ccaatccttg gaaatactcg ccgatttgat
     3721 aaaatagcat cactgctacg gcttctggat attcaccgat caacatagcg ccaatcgtag
     3781 caacagacat aagaaaattc tcatcaaaga tttttccttt gacgatattc atcactgcct
     3841 tcttcactac atcatatcct atcagcagat aaatcaataa ataccctatc aagcgaattg
     3901 gttcattaaa atcaaataat agcaataaag cgatacctgc aagggaaaat aaaatccgga
     3961 acagcgtatt tctctctttt tcatttccat gttcagatac tttttcctta tctatttctg
     4021 ttactttgac atctggttct aattccttca ctactttttt tgtttcttgt tcaatcgtag
     4081 ccaaatgatc ttcctctgcg tcaatcgtta gttttccaga agtgaagttt accgttgctt
     4141 ccttgactcc attgatcttc tgaactcctt tttcgatttt cattgcacag ttcgcacaat
     4201 ctaaaccttc taatctgtaa cttttcattt taatgacctc ctcgcctcaa catatgaaca
     4261 tctcttcata actaattata aatgaatggt tattcatatg tcaaggtatc cgaaagaaaa
     4321 aatgaaaaac atctgcatcg gaaaatttct tccatacaga tgttttggct tattctttct
     4381 ttttctcttc gatcattgcc cctgctctta tccaacccgc acttgccttc ttgatcttga
     4441 caggatagca actaagtttg aagcctgttg ctggcaattt gtctaaattc attaattttt
     4501 cgataggaca atatcttttt tcaatccctg caaaatgtcc ttcccagata ttcgaagctc
     4561 cactacgctt tcaaaaatat agaaagagtt ctttaatgaa aattatttca actttccata
     4621 aacagtaatc ccatataaat aaaagacgta aaagtcacat gggagaactt atacaggaaa
     4681 acacatagag tcaggtgaaa tacatgtatc ccacctgact ctatgcgttt tctaaccttt
     4741 attattctct ttttatacaa tttctgcacc gaatggcatc aacgccaatc gagcaagttt
     4801 gaaagattgc ttggcaaaag gtatccctat gatcgtgata catagaagga tgccgctgac
     4861 gacatgtgcg agagcaattg gtaggcctga aacgatcagc cagatgatat taacgatcaa
     4921 tgacatgcca gactggtgat aaattacttc tttgccaaaa ggcgcaaaag aaagggaggc
     4981 gatcttaaaa cattgcatac cgattgggat acctatgatc gtgatacacc aaagtatccc
     5041 cgctgctaac cagctaagac caccgattag tccgccgaat ataaaccaga tgacatttcc
     5101 taaacaactc attgtatttc tccttctatt actgtttttg attaaaactg ggcaaaaatt
     5161 gattttattc ttcttttttt agcttccagc atcaagtatt ctgcaaattt tttttggatt
     5221 tcttccgtat tttcttttgt tactaatgtg tgttggatta gttttcctga tggtcgctga
     5281 tccacccgtt cttctgactg ttgcacgtcc gcttgcgcaa atccgataat ccgtttttct
     5341 tttgttacag caacgagcaa tagtttattc ttatcattta ggtcagctgc cgtttttgct
     5401 tttacgaatg attctggatg atcagtaatc agctgattga tttgaggtaa atcttctttt
     5461 actgcccacc gtatttccat catttctcac ttttctttcg ctcattttta tctccactaa
     5521 aaaaatcgac tgcggcaatt gcatctatcc agctttgtgc aatgatctca tgtccgccaa
     5581 tcgttgggtg gaccccatct tctcttgtat atttcgaaaa accataagca atccctaaag
     5641 cattgaatat gccatctaaa ggaaccagca atgcgccata ttccttagca agtttacgaa
     5701 caatttggat tttagggtct aagtctttac gccagccatg acggtcgtcg gttataggaa
     5761 gtacaaacgg ctccatcaag atgatgttgc agatttccgc tgtttgaagt tcatttagta
     5821 attttcgata aacggattca aattgctgcg cagatttctc agttccgaat atagcctgat
     5881 cgctagtatt atgccaggta tcattgattc cgatcaaaat cgatacatag tcaggttgta
     5941 aagctaaaca atcttcttgc cagcgtactt gcagatctgc aattttgtct cctcctatcc
     6001 cacggttcat gcattcaaat ttcatcgttg gatattggga gattagtttt ccggcaatca
     6061 aaagtggata acctttacct agatcagttg cttctgctcg gtctcttcct gcgtctgtaa
     6121 tgctgtcacc gataaataat atacgttttt tcatttgtca aaaactcctt tttgaataaa
     6181 acgatgattt tcttccttat attatcccat aagccctatc aagacgaaag agaaaatata
     6241 tgtataaaaa atgacaccag cctagtccct tcaatcaatt gaatgaacgc tcatcaagtg
     6301 tctagttttt tatacttttt ttcttaacgt gccggattcc ccttgattcc ttcgttgttt
     6361 ggtccagaga aatcgatcac acctaagctt ccgtcaatca gaccactgat gacgccatca
     6421 gaacatatat ttataggctc atctaagact tccagtaaat gttcttttac taatatctct
     6481 tctagattgc ttgggataga ttggtcataa ctttatctgg tccgatgatt tcaaggatga
     6541 attaaactta catatggatc attgcatgct gaaaatacgg acctactcca tcttcgaatg
     6601 ttactattag tttgttttca ggtactagat gctgctttat ttcaacagca gaagcatcat
     6661 caaatcagat ttccatttga ataaccttct ttgtatatgt ttgattgatt gcgatttcgt
     6721 tatagaacct tgtgaatcaa atagcaattt cttttgcttt cttctcttgt ctgctggaaa
     6781 cttcctttat cccgtatttt aaaaaaagaa ctgcgagagt tcgcagttct tttttttaaa
     6841 taaacgttgc tctccggcca actcctcggt cacaagtcac aatagtcatc aaacatgaaa
     6901 aactgttttt tgaaattact ccttgtgact cggagctgaa cggcaggcgc acaacctctt
     6961 gatataaacg gtgttcaatc agctgaccct cgatattagc taaaaaaacg gtaaaatatg
     7021 agaagctatt tttccgtttt gtttctctaa tactcaggtc aaggcactga tttcacaacc
     7081 tcttccttaa acggtgttca aaagccagtt tctcggtatt aagccaaatt tatgcaaaaa
     7141 tgtggaaagc catttccaca aaatttttct taatactcga aactaaacag ctttttcaca
     7201 acctctccac taaacggtat tcaaaagctt acatctgcgg taataagtcc cgccaattca
     7261 aaaacatgaa cagctgtttt tgcttggctg ttctaattac ttgatgcaga acagcttttt
     7321 catcacctct tatggataaa ttcggttcaa caaacgtggg aatggaatag cttcacgtac
     7381 atggtcgata ccagcaatcc aagtgactgt tcgttcaagg cctagaccaa aaccagagtg
     7441 cggaacagaa ccgtatttac gtagatcaag ataccatgaa tattcttttt catccaaacc
     7501 gtgattacgg atttgttcta gcaagaaatc atagtctgtt gcccgttcgc ttccgccaat
     7561 aatttcaccg taaccttctg gtgcgatcat atctgcacaa attaccacgt cttctcttgt
     7621 tggatgtggt ttcatgtaga atggcttgat ggcttttgga tagtttagga taaagacagg
     7681 ctgttcgaat gaattcgcaa tgaacgtttc atgaggtgaa ccaaagtctt cgccccattc
     7741 gatgtcatca aatccatttt tcttcagtaa ttcgattgca tcatcgtaag taatacgagg
     7801 gaatggcaat tttgtataag tttttaagac ttctttgtct cgtcctagta catctaatgc
     7861 atattcgcaa tgatctaaaa cattttgtac taagaatgct acatattgtt cctgtacttc
     7921 taaactttct tcttgatgag tgaaagccat ttctggttcg atcatccaga attcgatcaa
     7981 atgacgtcgt gtttttgatt tttcagcacg gaaagtcgga ccgaatgaaa agacttttcc
     8041 gaaagccata gcagcagctt ccatgtataa ttggccactt tgagataaat aagctttttg
     8101 atcaaagtaa tttgtttcga atagatccgt agtgccttct ggtgcagaac cagtcaagat
     8161 cggcggatcg attttgacaa aatgatgttg attaaagaat tcatacgttg cacgaatgat
     8221 ttcatttctg atctgcataa tcgcatgttg tcttgaagaa cgcagccata aatgacgatg
     8281 atccatcaag aaatctgttc cgtgttcttt tggtgtgatc ggataatcgt ggctctcgct
     8341 taccacttcc acatttgtaa ttccgatttc ataaccaaat tttgaacgac tgtcttcacg
     8401 gatttctcca gttactaaca cggatgtttc ttggtgtaga tttttagctg tttggaaaac
     8461 ttcttccgac acttcgcttt ttacaactac accttggaaa aaggctgttc cgtctcttaa
     8521 ttgtaaaaat gcgatttttc cgcttgatcg cttatttgcg acccaagcgc cgattgttac
     8581 tgtttctccc acatggtttt tcgaatcgat gatttgaatt tgttccacgt atgactctcc
     8641 ttttatattc cttgaatttc ttcaggatta attttgactt ttactctcaa taaattgctt
     8701 gattctgcgt accgcttctt ctaacgtagc gcgatcagtg gcataactca tgcggacatt
     8761 ttctggtgcc ccaaagcctt cgccagtcac aagcgctacc cctgtttctt ctaaaagatc
     8821 ttctacccat tgtgtgacat tttcatactt acacatgcgt agcgtttctt ttacattagg
     8881 aaaaagatag aaagctcctt gcggcttttc aagagaaaca cccggcagtt ctgccacaag
     8941 cggatataac gtattcaggc gttcttcaaa ggcgttccgc atttcttcta ctgtatcttg
     9001 ctcacctttc aatgcttcaa cagctgcata ttgactgact gctgtcggat tgctagttga
     9061 ttgggaagca acagcgatca ttccattgat gatcacagga tttcccaccg cataaccaat
     9121 gcgccagcct gtcatcgcat atgttttaga cacgccgttg atgatgatgg tctgattttt
     9181 gattgcttct gaaatcgttg caattggagt aaactcattg ccattataaa ctaaacggcc
     9241 ataaatatca tcagcaacta tcaaaatatc attttttact gcccattcac cgattgcctg
     9301 cagttcattc ttgctataaa tcattccagt cggattcgat ggagaattga tgattacggc
     9361 ttttgttttt tctgaacgtg cttgttctag ctgttcaaca gtaactttcc agctattgga
     9421 ttcttcccct ttcacaaaga caggaagtcc ttctgccagt tttacttgct caccgtaact
     9481 gacccagtaa ggcacaggaa tgatgacttc atcttgcggg tcaaggatcg tttggaaaag
     9541 ggtatacaaa gcaaatttcg ctccgtctgt tacgactgtt tgtgaaggtt catagacaag
     9601 tccatagttt ttcttcagat aatcaacgat tgcctgtcga agctctggga ttcctgctgt
     9661 tggtgtataa tagctagctt tcccattgcg gattgcttcg attgctgctt cttggatgtt
     9721 ttctggtgtc gcaaaatccg gttctcccac agtcaaactc agcacatctc tgccttttgc
     9781 ttttaatgcc ttggcctttg ccgcagctgc caaagtgaca gacggttcta atcgttttgt
     9841 tctttttgat aactccatcc cattcattcc tttacctttt aaattccctt gattgtcttg
     9901 acttcatctc cagattcaaa gccaatcagg taataattca gtttcccatt tgtttctttt
     9961 cctactactt cccacacagg ctgatcatca tacatcccta aagcagcttt ttcgatttgg
    10021 atttcaggat gggccgcagc aattgtctgt tttgcttcgt tttcagtcag tccctttgtt
    10081 tgatccaata cttttacttt ttcaccagat ttaggaatga taacggcgat ttcttttcct
    10141 ttatcatttg tacctgtcag tgaaaagtat gtcttctttc gagtaaacca ataaaaatga
    10201 tcggtttcct caagatttgc atatttcttt gcgatttcta ctgcttcttt cttcgcctgc
    10261 accgccggac gactggaacg caagtaaaaa atcgagctga tcacgatgac cgctaataaa
    10321 aatacgatca agccaagtaa cacgcgatta atcgtttttt cctttgtctg ttttttcttc
    10381 aagggtcgtc gctcctttat cttcattcct tttcattata gcagatttta attatgaaaa
    10441 aagacagctc ctctaacttc cttcttcatt tttctttaag aattcatgga tatcctgata
    10501 gatttcctcc atagctagct ctttgatcgg tacttttttc ggtaatgcat ggatgatccg
    10561 tgcgccatat tttgcagtga ctagtcttcg atccaaaata agcaatacgc cacgatctgt
    10621 ctccgtcctt atcaagcgac ctaatgcttg gcgtaacttc aaagctgctt taggtacgga
    10681 ttcctggtaa aaaggattga tcccctctga ttctaaataa gcgttcctag ctttgaccat
    10741 cgggcgaagc ggattatcga agggtaaacg tgtgactaca acgatctgca aagcatctcc
    10801 aggcaaatct acaccttccc aaaagctgtc agcgccaaac aatacgcttt tttctgacaa
    10861 aatgaagcgt ttcaaaagct tctcccggct tccaccaaac ccttgagcta gtatttctcg
    10921 tcctttttct agcatagaca gatgcatttt ctgatagacc ttctgcaaaa catcatgaga
    10981 agtgaataac acaagtattg gccgatcttc ttgttcgatc agacctgtaa gtgctgtctc
    11041 taaataatcg atatatgctt gattgctgac ttgatggacg gcaggtgcct cttttggcac
    11101 atagatcctt gcctgtttct cataatcata aggagaggca atcactttaa gaggtgctgc
    11161 ttcaatgcca agcctcttag agagatagtg tcgattaggg ccgatcttca gcgttcctcc
    11221 aatataaaga atacgatcat atcgaggata ccaaacagtc gacggcaaca tagccgcttg
    11281 aaaatcatgg agatgaaaaa tcatctgctg cgctgtgttc ccaggaacga accaatggat
    11341 caaatgatct cgccagtctt ccagccattg atccatcagc aagtattgct tgtctaattg
    11401 atcaaatagg ctatataaac ttgctaaatc gatccgcatc tggtttgtcc agctttcttg
    11461 gataatctca aaagtcagac gcatttgtga ctgtagctgc tggatctcct gataaaaaag
    11521 ctgcaggtga tcaatggctg tcccagattc cagtggcagc tgatccaaca tctcttttgt
    11581 catcatgact tcttctggat actgtccgtt aggttgcttg ccgctcacta aactcattaa
    11641 tccttctgcc atacaaatct gtgcttcgca tagcgcagaa agttcttctc gataaatcga
    11701 taaccagcgt aaggcttcat gttctttcgg taaatagctt tgtattcggt caaacagctg
    11761 atttggttct tctaattgct ggatttgttt atggaaatag ttaatactta atcgtttgtc
    11821 atttactttt tcagcgattt ccggcaaatg atgtacttca tcgatcaaca aaaatggact
    11881 agctggaagc aatggttctt ttcgctgtgt ttcttgtact aaaaacgcat gattgacaat
    11941 caaaaaatta ctttgcctga ttttcttttg caagaaacga acaaagtctt gttcgtaaaa
    12001 tgactgattt cttgctaaaa atccaatacc tctgtgcgcg atatctgcaa agaatgaatg
    12061 gttcaaattt gttaaatgta attcatccaa atcaccagtc ttcgtttgcg tcagccaaac
    12121 taagaccgcc atttgataca aagcatattg tttttgttct ggcggttcca caagtgtgcc
    12181 tttaaaacgc tgcaaatcaa tatagtggcg atagcttttg ataattacag gttgtatggg
    12241 ctgatctagc aattgattca gcaatggaat gtctttttct atgatctgct gctgcaaaac
    12301 tagcgaaacg gtactgatga cagcgggttt ttcaggtgta gccaaataac tcaatggcag
    12361 cagataaccg attgttttac ccattcctgt agctgcttcg atcatcaaat cttttgattc
    12421 atcttttgtg aaatggtcgt agaccgcatt catcagtctg ttctgttctt ttcgatacat
    12481 cagtgtttta ccaaacattt tttcttttgc ttgttttttt cttggatatg tccgctcgcc
    12541 ataatggacg gatgtgaata gttccacttc ttttttctgc aaagctagcc catcgacgat
    12601 ttctaatgag ggatcaagtg gctctggttt ttctttcatt tcttcaatca catgataaat
    12661 gaaacgactc gtatccatac ctgtgtgtct acttaatcgc gcaatctgct ctaacgtgat
    12721 gattggcaat tttctcatcc gttcttcgat cagcaaaaga agttgacctg tcacttcggc
    12781 atcactatct gcctgatgag gattctcatg aataaatcca aggctttctg acaaatcagc
    12841 taagcgaaag cttggttccg tcggcaagaa aatttgtgca agttctactg tatcgatgcc
    12901 tggtatcttc aaactaggaa gtccgcatcg catgagttca tggttcaaaa aattataatc
    12961 aaaataaata ttatgcgcga caaatatggt gtctgccaaa agattgtaaa tcgtttgggc
    13021 gacatcttcg aaataaggcg ctttttggac gcgttgattc gtgatccctg ttaaatgctg
    13081 gatttgtttt gaaatcttcc gccctggatg aatatcgatc gaaaaacgtg aagtgatccg
    13141 acctgattca atcatgacgc agccaaattg gatgatccga tcttcttttg gattcgtccc
    13201 agtcgtttcg atatcaacga ctgcataagt tcttggtttc aaaatatctt cctcttctcc
    13261 tgcctctaat gttcttttga aattatacta caaagtgcag aaaaatactc tcatcgcttt
    13321 ttaaagaaaa tagacaaaaa cgcccagaca cgcaaatgtc tgggcgttta ttttttagta
    13381 taggttaatt aagctttagt aacgtttgtt gcttgaggtc cgcgttggcc ttcttcaaca
    13441 tcgaaagtta ctgcttggcc ttcttctaaa gttttgaatc cgtcgccttg gatagctgag
    13501 aaatgaacga atacatcgtt tccgttttct gcagtgatga atccaaatcc tttttctgag
    13561 ttaaaccatt ttactgtacc tgtttccata ataaaaatcc tccttgtgta tcaacacata
    13621 ttttgcaatt acttgaatgg tgtatatctg gaagatgttt atttgaaaca agctaccaca
    13681 gattaccgaa caaaaactac tcctatagta tatcataccg tttacaaaaa agctagagtt
    13741 tttcgacttt ttttattaaa aaaagttttc tcagcgatta cataaagtga aaaaccgaac
    13801 ttttttttaa aataaacgac atattgttca gttggtgttt ttttgctctg atgttctttt
    13861 ttaaactaaa aaaattattc tttctgacta cttatcggtt ctgtttcaac ggtcgaggct
    13921 ggggaagatt ctttagtagg tctgatgttc tcatggtatt tctgatagcc tatgacagtt
    13981 accagaagaa caaggcaaac caatacgacc aacgagatta gtttttcttt tttgctcata
    14041 tctatacctc ataaataaca ggatgatgtc catcatattt ctgagtatga gtaaaagcaa
    14101 aagctgcagc tgtagaatgt aagaaatgcc agcaactttc ataatcacag gtcggacagt
    14161 accaaaagta accaaatctt ggctgcaaag accgttcttc tgtttcagga atcgcagaat
    14221 caagatctgg tgtctgtaca taaacatttt tgtacagacg tgtgcctgta ataggaaacc
    14281 gctgtgtttc ttcagcaaaa actggctgac ttgttaaact tagggataga gacagcaaac
    14341 accataacag tctttttttc ataaagacct ccttatcgat aagtttttcc caattaaaat
    14401 catatctcaa gaacgcccgt tattcacatt ttgttcataa atgatgtttt ttgtatcata
    14461 atcgtcaaaa agaggtcgtg accacgtcac aacctctttt ttaaacggtg ttccagaagt
    14521 agcttcttcg gaaataagcc gaaatttcac aaaaatttga gaaataattt ttgtcaatct
    14581 cttcttattt cttgaagctg accacttctg tcacaacctc ttccttaaac ggtgttcaaa
    14641 agcttgcatc tgcggtaata agcccaccaa acaaaaaata tgaagagcta tttttgcttg
    14701 ctgttcttat tacttgatgc agaacagctt tttcacaacc tctcatacac ggtgtttaat
    14761 cagctgacct tcggtattag ctcaaaaaac ggcaaaatat gtgaaactat ttttccgttt
    14821 gtttctctaa tactcaggtc aaaacactga tttcacaacc tcttttaata acgaagaatc
    14881 gctgtcagac tcttttcatc tggcgcatct ttttgatcca aaataaacac ttttccgcca
    14941 ttaatgatca catcatctgc taatgcattc aacacttgac gccgatcata ttctgtttca
    15001 ggatcttcac cgaaaccatc agctaagttt gacgtagcga taaataaatg tgacactttt
    15061 ccttctttcg cagctggaac gatatcaact aactgatcga tgaatttctt atctaagagt
    15121 ttattgtatt cttttgattc ttgagctgcg agttcacttg acagcttctt cgccccttct
    15181 tcgatatctc taaaggatag ctgcgcagga gaagcagaaa tcgcaacgtc tgcacaataa
    15241 tagggatttt tagcaatttt tttgaacatc gtctgatttt ctggtagagc aaataaataa
    15301 atcggccatt gttctggatt ttcgaaagaa tccttcaagt acgtatcaac tgcttggtaa
    15361 tagttgaccc aatcgatctc tacttcttca tcttttggat ttacgccatg gaaagttact
    15421 ccatctcggg ctccgcctcc tccattagaa gagaagttca aaccgccacc tgtcagttca
    15481 gatcccaatg cagtaacgac atcaaccggt gccccttctg gtagagatac ttcttttact
    15541 tgtttgttgc tgacttcata caatttcatg gagtcgcggt tcaatcccaa taaataataa
    15601 gaatagttga actgcccatt tttgataata gccagtaaat aaggaagatc acttacatag
    15661 tattgatcgt ctaccgggat actcaaacga tggatatagg tttggtcagc agttataatg
    15721 accgctacac tcgtagttgc actacgccaa aacgaaggat ctcctaacaa attttcaatt
    15781 ttttcttgga acggggacca gtcatgttcg gaatattttt tctcaaaacg tttctttgct
    15841 tctttcgcaa aatttttgaa tttgatctga tctttttcca catcttgatg ggcgacatgc
    15901 gtattcagca tgatcgtgac gaatggtcct ttagcgcctt ctgaagtcaa ttgtgacaat
    15961 aattctttat ccttttttgc cataaacgta cccctcctaa aaaaatcatt cctttgtttc
    16021 tatctaaagt ctaccacaac actatttttc catacaataa aacgtatcta aaaaaataaa
    16081 acggtttcta cggttttaaa tttagctatt tttttaagtt atcttcataa tttcctcaat
    16141 tatttagccc cagtcttcaa ctattcttca tgaaatgctg ataaactaag aaaaagaata
    16201 aaagaggtgg atcttatgca tttgattatg attttcagtt tattaagtat tatcagttta
    16261 tttgctttca gcgcttcccg cttattagat gcggaaacac agcctattcc tgtaaaagta
    16321 cgtaaaaacg accgctaacc ttccataacg tgcgttctta tcctaaagtt ttttcagaaa
    16381 cattgaaact ctctcccctc tctgataaaa ttttaatttg tagcgaaaca aattaattac
    16441 aaggagatcg gatatgtcca taaaaaaaac acgtctagtc ctattattgg ctagtctcat
    16501 tctttgcctt gcttcttttc taccagctgt tgctgtgcaa gctgaagata ctttcaaagt
    16561 aaatgcaaaa gcagcctttg ctgtagatgc gcaaagcggt aagattcttt atgatcaaga
    16621 tggagaaaaa accatgggga tcgcttcaat cacaaaaatc atcggtcttt acatcgtcct
    16681 tgatcaagta aaagaaggga aactatcatg ggatgataaa gtttcaattt cagattatgc
    16741 agaaaatttg agtgtcaccc cagatttatc aaatgttcct ttgcataaag aaaacaccta
    16801 caccgttaaa gaattattcg actctgcaat catccagtca gccaacgctt caatggttgc
    16861 cttggcagaa aaaatctctg gcagcgaagc aaaattcact gaacgaatga aagaacaatt
    16921 gaaagattgg ggtatcaaag atgcaacgat cgttaatgca tcaggactga ataactctta
    16981 tcttggcgaa aaccgaccag aaggtactgg agaaaacgac gaaaatcaaa tgtctgctca
    17041 agatgtggct atcgttgccc gtcatttgat cttggatttt ccagaaatac tggatgtatc
    17101 tagcactacg acacaaatgt ttggcgaaaa cacgcaatca ccagtagaaa tggtcaactg
    17161 gaactggatg ctcccaggat tcgtgaatta taaagaaggc gtggacggat taaaaactgg
    17221 tacgactgaa cttgcaggag cctgtttcgt cggtacgatc actaaaaacg atcaacgaat
    17281 catcactgtc gtattgaatg cagacggcca tgcggaaaac ccaagtgccc gtttcaatga
    17341 aacagctcgc ttaatggatt attgctacga caattggtca gaaaaagagc ttggaaaagc
    17401 caatgcaagt atccctagtc taaaaaccat tgatgtaaaa gacggaaaag aatcaacagt
    17461 gcccgttgtg cttaaaagtc ctgtaaaagt atgggtacgg aacgatatgg atactggaaa
    17521 attaacaatc acaccaactc ttgacaaaaa acaagtcact gacaatgaga tccaagcacc
    17581 tatggataaa ggaacaaaaa tcggtaccgc aacgatcacc ttggcagaag ataaattagg
    17641 ttatcttgaa gacaacaacg cgccatccac agaaatcatc acggataaaa ccgtagaaaa
    17701 agcaaatatt ttcgttattg gctggcgacg cgtcgcagaa tttttcggta acttgtttta
    17761 attgaacaaa aagaaaaaag catctttcca tgtggaagat gtttttttgc tttttctagt
    17821 ttaaataaca gcttttttca tctattttcg ttatactgta taagaaaaag gagttgaatt
    17881 tatgaatcta accagagatc tgattttttt ggttctctat cttattgggg tattctccat
    17941 cgtttattgg cgaaaacaga aaaaggatcc ttccttgttc ttttacagca gtacatggac
    18001 gtttgcggta atcttgactt attttgtcgt gttggaaatt ttttattcga ccttttattt
    18061 tttgattccc ttagtgtttt tcagtatctt tgccttttcc tatttttcag aaaagcgaag
    18121 gctgttgaat ggtcttttat tcaatatttt cctcattagt tttggtatct acttgtttgt
    18181 cttgctatac gaaacacaaa atatttttct tggcggactg attgccttga tcacgatccc
    18241 gttactgctt gtctttctat tcggcattta tggattgatc gtttttcttt tttggaacgg
    18301 tgtcacagtt ttacgtagag agtcgcattc attagctaat cttttaacgt tgatactagc
    18361 catttttctt acattgtttc ttgtttttga ctttttcttg ttaaaatatt taccacaatg
    18421 gataaatgct ttgtttttct gcatcccatt gatcttgatc tatctattta tcgtattcta
    18481 caactttttg actgtctcat tcttgtatca gttcaaccgc cctcgataca accaagactt
    18541 catcgttgtg ttaggcgccg ggttgatcaa tggagaaaca gtttctcctc ttcttgccaa
    18601 gcgaatcaat aaggcgattg ctttttattg ggcacaaagc cgtgcgacat taaatccgcc
    18661 tatcctatta atgtcgggtg gacagggagc agacgaaaaa gtaccagaag caatggcgat
    18721 gaaacaatat gccatggaac aaggaattcc tgaaagagat attcttgtcg aaaccaattc
    18781 gacaaccact ttggagaata tgcgctattc aaaggaaatc atggatcagc aaatgaaagg
    18841 tccctatcgc gcgatcttca gttccaacaa ttaccacatt ttcagagctg gtctctatgc
    18901 acgacaagcg aaactgaagg caaatggcat tggggcaaaa acagctttct attacttgcc
    18961 taacgcgttt ttaagagaat acatcgccat cttgatgatg aacaaaaaaa gacatatgat
    19021 catttgcgga ttgcttttcg caggttccgc attcttatca ctgatcactt tacttttcta
    19081 aaaaa
//
LOCUS       DADVXU010000052        18821 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.054, whole genome shotgun sequence.
ACCESSION   DADVXU010000052 DADVXU010000000
VERSION     DADVXU010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 18821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.054"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            18..1469
                     /locus_tag="KB953_001686"
     CDS             18..1469
                     /locus_tag="KB953_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HBB6866664.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1818..2450
                     /locus_tag="KB953_001687"
     CDS             1818..2450
                     /locus_tag="KB953_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HBB6866665.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            3038..4477
                     /locus_tag="KB953_001688"
     CDS             3038..4477
                     /locus_tag="KB953_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB6866666.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4772..4990
                     /locus_tag="KB953_001689"
                     /pseudo
     CDS             4772..4990
                     /locus_tag="KB953_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5239..5454
                     /locus_tag="KB953_001690"
     CDS             5239..5454
                     /locus_tag="KB953_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866667.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5671..5927)
                     /locus_tag="KB953_001691"
                     /pseudo
     CDS             complement(5671..5927)
                     /locus_tag="KB953_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6114..7025)
                     /locus_tag="KB953_001692"
     CDS             complement(6114..7025)
                     /locus_tag="KB953_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBB6866668.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(7044..8048)
                     /locus_tag="KB953_001693"
     CDS             complement(7044..8048)
                     /locus_tag="KB953_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBB6866669.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(8045..10168)
                     /locus_tag="KB953_001694"
     CDS             complement(8045..10168)
                     /locus_tag="KB953_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBB6866670.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10173..12620)
                     /locus_tag="KB953_001695"
     CDS             complement(10173..12620)
                     /locus_tag="KB953_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBB6866671.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12607..12996)
                     /locus_tag="KB953_001696"
     CDS             complement(12607..12996)
                     /locus_tag="KB953_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBB6866672.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(13056..13559)
                     /locus_tag="KB953_001697"
     CDS             complement(13056..13559)
                     /locus_tag="KB953_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBB6866673.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13572..13796)
                     /locus_tag="KB953_001698"
     CDS             complement(13572..13796)
                     /locus_tag="KB953_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866674.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(13900..15810)
                     /gene="ltrA"
                     /locus_tag="KB953_001699"
     CDS             complement(13900..15810)
                     /gene="ltrA"
                     /locus_tag="KB953_001699"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBB6866675.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(16556..16693)
                     /locus_tag="KB953_001700"
     CDS             complement(16556..16693)
                     /locus_tag="KB953_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBB6866676.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            complement(16690..17874)
                     /locus_tag="KB953_001701"
     CDS             complement(16690..17874)
                     /locus_tag="KB953_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBB6866677.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(18133..18423)
                     /locus_tag="KB953_001702"
     CDS             complement(18133..18423)
                     /locus_tag="KB953_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866678.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(18413..>18821)
                     /locus_tag="KB953_001703"
     CDS             complement(18413..>18821)
                     /locus_tag="KB953_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HBB6866679.1"
                     /translation="RVYEVTGTAPTLSTKQGGTKIMIRKNETEYQEVKPEDSVNLAFP
                     NSTSRRGRLGKQSVHTLLTGDQQAIVTDQYQIRKLTPRECWRLQGFPDWAFDRDSQVN
                     SDSQLYKQAGNSVTVPVIFDIARRLKELDVHGL"
BASE COUNT         5547 a         3823 c         3333 g         6118 t
ORIGIN      
        1 aaagagaggt aaatcaaatg aaaaaatctt taaaacttgc atcagcagtt atattaatgt
       61 cccaactcct tatagcaaca cctttccatg tgttggcaag cgaaaattca acatacaaaa
      121 ttgcaaaaga aagaaccgag acgactgaaa tgagtaattc cgatgtaaca gcagagagta
      181 gtgaactttt gacaacttca tcaagtgaaa gtagtacgac ttcaaatgcc gagtcttcaa
      241 caacttcatc aagtgaaagt agtacgactt caaatgccga gtcttcaaca acttcatcaa
      301 gtgaaagtag tacgacttca aatgccgagt cttcaacaac ttcgtcaagt gaaagtagta
      361 cgatttcaag taccgaatct tcaacaactt caagtagtac aagtgaaata acttcttcga
      421 aagtgacaaa accaaaccat atagaatctt caaaaaagga agcgccaaaa acggaaggac
      481 aaacaaaaac tttttcgaca ccacagcaaa tagaatcaac agaatctttc caatcacaac
      541 caatactatc tggtaatgaa gaaattcatt ttgacaaaaa tgaatctgta gaaaatttta
      601 ttatgaaaat tggtgagtct gcgagaaaaa ttggtcagaa atatgattta tatgcttctg
      661 ttatgattgc acaagcgatc ttagaatctg ccagtggtca aagccagtta gctcaggcac
      721 cgaattacaa tttatttggg ataaaaggaa cttataatgg aaactttgtc attatggtaa
      781 caaacgaaga tttgggtaat ggaacgcttt atacgaccca atcaaaattt agagtgtatg
      841 aaaactatga ggaaagcttt gaagattatg ccaaattatt aactaaggga attagtggaa
      901 ataaagattt ttatgcagga gcattaaaag ctaatagtaa aacatatcga gaagcaacta
      961 aattccttac aggacggtat gcaacagata cacaatacta tttgaagcta aatgaattaa
     1021 ttaaaaccta cgatttaacc aactatgata aagaagttaa aggtccacaa atgagtaaga
     1081 ctggatacca agtgcctatt cataattatt caataaccag tttgtttgga aatcgtggaa
     1141 atgaatttca ccgaggactt gatatggcag ctacacaggg tgagccgatt catgcagtta
     1201 aagctggtaa ggttgttaaa gcagagtttc actactcttg gggaaattac gtggtgatac
     1261 aacacgaaga tggatcaacc gcgttatatg ctcaccagca acaataccta gtaaaaactg
     1321 gagataatgt tttgcaagga caaataattg gctatgtagg aagtactggg aatagtactg
     1381 gcccacacct tcatttggaa atttgcaaag atagtagtct ttctcagagc atgttagttg
     1441 atccgaaaac cttattattc ggagactaaa aaaggtaatc aatctgacaa agtaacaaga
     1501 aaatggttgg attcttagac ctttgatcag gatattgaga ctggaattga tatctcgatt
     1561 acgatgggtt tgacaaataa aacaagtcca ttatcgaata tcgagtgtat tattgccgtc
     1621 tttatgctca tattttgagc aggtttcact agatgaaaac aatttatcta tcttgaggat
     1681 ttcgtgtacg tatcgttcag tcttgtattg taaattagtc ataaaatcag actaagaaaa
     1741 attggaaata tttttttctg atttattaaa agttaattga aattatatgg tacttctttc
     1801 ttgggagggt aggtgcagtg aaggaaagat atggtcacct tactgttgtt gaggagatta
     1861 ctcataatgg atataaaaaa tatcgttgcc tatgtgactg tgggaatgaa acgattgtat
     1921 atcgatcaaa cttactatca ggtagaacca aaagttgtgg atgtggaaat tataaaaata
     1981 ggcataaata taaaaatttg acaaataaaa cttttggtaa gttagttgca atcaagccaa
     2041 ctaaactgcg aagtgaagga actgttgttt gggaatgtca atgtctgtgt gggagaaaaa
     2101 agttagtatc ggcaaagcga cttctaaaag gtgatgtaaa aagctgtgga tgtttgaaat
     2161 caaaaaagca tcacattgaa aataaaaaat tcggattact tactgcaaag tatcctttgg
     2221 acaataactg ttcatggaaa acaaaatggg tttgtcattg tgagtgtggc agtatgtgtg
     2281 agataagtta tagtaactta ttttttggac atactcaaag ttgtggatgt ttaaaaaggc
     2341 aagaaacaag gacatgtgtt gaagggacag taatagagtg tctaacgtca aaattaccga
     2401 aaaataatac aagtggtgtt aagggagttt atcgttctaa agagaaatag attgcgtata
     2461 taactttaaa aaaaaagagg tactatttag gatcgtatga ttctattaag agtgcagctc
     2521 aagctagaaa agatgctgaa aataaattgt ttcaaccttt gattgacaaa tatcgaggta
     2581 atcattaatt attgtggata ataattaaca gtgatatata aacatctata atatattatt
     2641 catactattg atatttagta taatcctatg gaattgaata tcatagcaat gtatttgaaa
     2701 agcaaattgg aaaacaaata ggtgcaacac cataaatatg aaaaaattgg gaaaaagtag
     2761 aatggaaatt tgcttacgtc tcacgagata gctcacacta ttaatctgaa gaacaatgaa
     2821 gtcgactttt agaaattgct agaagaaagt aaagcgaatt atcactagtt actatagagt
     2881 aagttcaaat aaagcatctc caacttacat ggttacatga ggatatcaaa tcgaaattac
     2941 tcaagcttcc tatggctaga gtcgcgagaa gttgttgggc agtagacata ggttatattg
     3001 ttcttagcta cacgatcatt agaaaggaaa tcctattatg tatagttatc aagaagatgc
     3061 aaaagtccaa aaaaatcgct ggtggatttt gattgctata tctatgttta ccttcatgtc
     3121 cacattggat ggcagtattg tcaatattgc actacctacc atctcagatg atatgggcat
     3181 accaatgaat caagcggaat gggtagtgtc cgtttatctg atgattgtct gttcttgttt
     3241 gttgctgttt ggtaaaattg gcgatagccg tgggaaaatc aaagtatttc ggattggtac
     3301 gctgatcttt gtcttgggat cgctgttgtg tgggttcaat cattcgctag gtattttatt
     3361 gttcgcacgg gtggtacaag ccattggtgc tagtatgact atggctacaa atgccggaat
     3421 catcacggaa gtctttccca tacatgaacg tggtcgggca ttgggagcaa taggtgcgtt
     3481 tgtttcatta ggctcgattg ctggaccagg tcttggcggc ttgattttgt cccaattttc
     3541 ttgggcgtac attttttgga tcaatgtacc ggtgggaatc gtaacgatgt tgattgctga
     3601 aaaattctta ccaaaagata tcaccatgag cggccataca atcgatggag ggggctttgc
     3661 tgccattgcg gctacgatta tcagcttttt tggagcaatc tttttggggc aagaaagtgg
     3721 ctttttccat ccagtccccc ttgtactgtt cgtaatttca gtgattgcct ttgtcagctt
     3781 tttaaaagtt gaaaagaaag cagcctctcc attgatttct ttcggtattt ttaagaacaa
     3841 gatttttacc atgagcttga ttacagcctt gttgattttt tcttccaact tctttgtcaa
     3901 tgtagtgatt cctttttatc tgcaaaatgc tcgcgggttg agtcctagct atgctgggct
     3961 gttgatgatg gtctttcctt tgatgatggt cgtcggggcg cctttaagcg gctacctgac
     4021 cgaccgggtt ggcccggaga ttttggtatt agtaggcttg ggcttattgt cggtaaccca
     4081 ggtcatgtat atgtttttgt cagtggactc tcctttgtgg ctatacattg tcgctactgc
     4141 catcatgggt gccggcaatg ccttgttcca gtcccccaat aatacgatgg ttatgagctc
     4201 ggtcactatc gaaaacttgg gactggcagg ctcgatgaat tcctttgccc gaaatttggg
     4261 gatggtcagc gggatttcgc tttctacgac ggtcttatat caagcgatgt ctgctaagat
     4321 gggccagcaa gtaactaact atatcgcaaa tcggccggat gtctttttat atggaatgaa
     4381 gattaccttt ttaggcagct ttgttttgtg ttttgttgcc tttttcctga ctatctggcg
     4441 tatcaaaaaa caaggaggta ttcagaaaaa tgtctgaaaa aaccactgtt gaagaatttt
     4501 tggaggcctg cgtatgggtg agtgggattt ccatggatga tggaagatga agtgaacttc
     4561 taaaagccga aaaactcaag tattatcttc aaaatactag tgctcaatga gatgcttgat
     4621 ttgatcacaa gaagactgcg aaagccgctg gcaaccgcaa gctaggtatc ccaccgatgg
     4681 actacgtttt agaggctact ggttatgaat acggagccaa cgctctcatc tgcataagaa
     4741 tacatcaccc agattatgta aagattttcg atacttctgt caaaaaatca ttaagataga
     4801 taaatgattt ccgtctagtc aaattggttc agaatgtaga cacacagatg gtaagaagtc
     4861 actcacaatt cgagaatgga cttgtcctat ttgccgtata catcataatc gaaacgtcaa
     4921 cgatagtatc aatattttga ccaagggcta tgaatacaag cattgcctta gaaaacatca
     4981 agaaccttag gaacaacggg gatagcttgg ttaataagag ataccgctgt ttaggcttgc
     5041 aagtatactc ttttatcaag aattccgtgg ctttagtcat gagaggctta atactatgaa
     5101 aattttcttt agcatttttg atttaggttc cttttctaat tataataaga tgaccactac
     5161 agtaacctac tgaaatatat aaacagccac tgtagtacca ttttcaaagt agtctatgag
     5221 aggagaatct tcattcttat gaacgctgga attgcggata tgaatttaat aaaaaaaacc
     5281 cttaatgatt ttacttccaa ttcaatatca aaaggtactg gtatcaattt aagcacaata
     5341 aaaaagctga aaagtggtga aagatcagtg gaaaagctga atcttttaga tgctataaaa
     5401 ataacggaat ttgctatgaa aaatggaaaa gctgagattg aaatatggag ataattatcg
     5461 tctagtgaat tacttgccac ttagctgaat ttaacgatcc ttggtgcttg aagggggaga
     5521 ttcttgggaa tagagcatac ttgcaagcct atgtctttac taacaacgat atctcttatt
     5581 aaccaagcta tccccgtagt tctaaggctt ttacttttat ctaagcaata atcgtattca
     5641 aaggaaattt ttgaccctta gaaagtctca ttttatcaga cgtgagctta ggcattgctg
     5701 aattttgtta attcggtttt aggttgtgct ttgagcataa tatgaaagta ttctttatca
     5761 cgatttcatt catctaaggt aatgtgatgt gattctgaaa ttctttcaaa ggcagttttt
     5821 gcataagctg acattacatc atcaatgaca tgtttacaat gtttcactac taaaataagg
     5881 gggtaaccaa gaagaaaaac tgaatgatta tttgtatcta attccatggt atatcaacca
     5941 tttcctatat agactgatta tgacatggat taaaataaaa aggcactatg tcttaattgt
     6001 cggttttcga gttaccgaat ggcattcatc acccacctat agaggatggg cgacttctgc
     6061 ctgaaataag ttacactact tataacttct aataaaatta gattgagtca ttattattta
     6121 acatcaacga ttttccagtt tccatttttt tgtaatacta atttgtactg aaagtagcta
     6181 gtggtttttg atacattatc aaggtatttt atctttgtac tcactagaaa attttttccg
     6241 ttttgtagat aaactggatt ctctaactcc gaaaacttta agtcaatatt gattggtcgc
     6301 atcgcaccat tttcaacata atactccaat tctttttcag aagctgatgg ataaagttta
     6361 aaaaatgttt ctaaaaaatc ggtgatttct tccgttgttt tggcatcaat ttctgaatcg
     6421 ttttctactt gttttgcttc atattttgcc ttgtctggct ttccaatcat tgttggcaaa
     6481 ctagttacta aatgattcct gttttcatct tcaaagattg tcactcgata tgcagaagaa
     6541 attgattgcg tcttttttcc ctctgtaata tgttgctcca ctaaaaatga aacgacaaat
     6601 tcttccgagt gtttttcaac atctagaatt tttacagatt gaacttcgga actcgtggga
     6661 atatctgctc gaaccatatc ttggattaaa gcgagaccgt tttctgtaag gtactgttcg
     6721 agattactca ttctcttttc aattgcttct ttgttatttt tccatgaaaa atattctttc
     6781 gcaaaatcct ctgtaaaact ttcaacgcca gaagtattca ctaccttctc ttttataacc
     6841 actttttcat gagttgtatg ttgatcaatg gcggtgaaat ttttataaac gccaaaagca
     6901 agactacaag acaataaaag ccaaagggca agcaccattt ttcgatgctt acccacggat
     6961 acaactcgtt cttttttctt ttttgggctc ttttcctttt gattccgttc aatttttatc
     7021 ttcattcttt ttcctctttt ctcttatttg tatcggccag ctccgataag atgctgccgc
     7081 caatagtttt ccgttagatt cgtccacccc actggatctc ctgcatgaaa catgcgattc
     7141 tcccctgcgt aaatgcccac atgggtcaca taagtaccgg catcataggt tgaatgaaag
     7201 aaaactaaat cacctggttt ggcatctttt agatcaatat gagtcatggc atcatattga
     7261 tcctgagcca cccgaggaag tttcaggtca actttggcat aacaccattg ggtcaagcca
     7321 ctacaatcaa accccgtttc tggacttgag ccaccaaaca cataggccgt tccttcatac
     7381 ttaaatgcct catcaaaaat ttcttggact attttgtcat caaactgagt tgtagttagg
     7441 tattgcttca ctaataaaca gtaaaacata ttgccataat tataccgcca cccaccattt
     7501 tccttaatcg cgattgggtt ggggtaatcg actttttgac cacctgattt ttcctttgaa
     7561 aaagattcag ctaaagcaaa actatgcttc ttcccgtttt tcccgacatg atctagatac
     7621 ccgccaccat agttataggc ctgaataata ctatcttgat cacaccctag ttctttggaa
     7681 cgcttaacca aagacgcaaa atagagacag ccttgtttga tggatgcctc ggtacttaat
     7741 gaattaggtg gtaatccggc tgactcagaa ctttgcataa cgtcttccat tgtgccacca
     7801 gattccactt gaataatcgc caaaagaatt ggaatatact ctgttatatc cgattctttg
     7861 gcatactttt ccaccattgc acggtgtttc aatacttctt ctgagacact agttccgcca
     7921 ggagaagtgc cagaactacc atttccctca ctgtcttcac caaacaacaa acccacacac
     7981 atcaaaatac ccataaaaga gatggtcaga aaagttaatg aaataacacc cactttcttc
     8041 aagttcattt tttctcacct gaccttgatt ttggacgatg gttcattctt ttttgtactt
     8101 tttgattggt ctgctttggc agagtggacc tctgtttcac ttgttggaaa gaatttttct
     8161 tacttgctga ttggcttttc ccttttgaaa tgatttcttg actagttagt tttcgtttga
     8221 tttcgggatt cagatttgct ctcacaatcc gctttggtgc ttcttttttc agcatcatat
     8281 ctttctgagg ttgagctgct ttatctattg gaatattcgc ccttcttttt ccctgacgat
     8341 ttgttgcttc actcagtatc ttgcgttttt cgtccatttt cgcatttctt cgttgtcgtt
     8401 gtttttctcg caaatccact cgatccgctt tttcttggaa cagtccgcgc ttgaattctt
     8461 ctggtgcttt tttcgacttt tcgattcctt tatggagatt gtatttcaaa ttcgttggcg
     8521 tattccttac ctgatccttt acttgtttag tcttatcttt caccaaagct tgggtatcca
     8581 aaactttgcc gatttttctc ccagccaaat tcattcggga ttgtttctta ttatttgaaa
     8641 ctggattctc cttagttaag tcagatagta tttcgtgatc gttcggtaat cgtgaattct
     8701 ttcgtagaga agaaccggat ggcttaaatt tggattttga ttgttccttt gccaacttat
     8761 ttccgactgc tgcaccacca ccaaaagcca aagctcgtcc cacatttcga ttgagttgcc
     8821 gcaattttcg attcattaac atttgcggtt ttcgcatgac gcgtcttccc attgactggc
     8881 tatcattact ctggaggttg aacatactca taatgtcgcc caatttgaaa tagataccgg
     8941 caaaggtcac aatttgcaga aaagcaacca tgaaaaatgg ataggttgcc gaaatattga
     9001 aaaacatcgt ggagatgcta aatgcagtcg taatcaccaa agtaacaccc gctcgcatca
     9061 taatcgtatt gaataaacga ataatcgcct tctttcctaa actttcataa gtcggcaaca
     9121 tggacaataa gaaactaatt ggcagaaaca tcgcaaagat aatgaagaga atttgcgaga
     9181 acaacataat ccctgttaga agaaaaacaa agaaggaaat gccaatattg aacaatacca
     9241 aaaagaccac ggtccctaaa cggttcatgg tcttggtaat actgagattc gcattgtcat
     9301 tgtcttcaat ctctgccttg accgcttctt cccgatcttt gcctttgttt tcatctggac
     9361 tcacagatag aatcttgttg acccggtctt caccaatttc ttctatgttg gaatcatcaa
     9421 attgcagtag taaccaaggt tgttggactt gaatcgaaaa caaactatcc cgaatcaaat
     9481 ccacactatc ttttccttgg ctttctgaat tgggtacaac gatctcggtt ccaagggtaa
     9541 gggccgcttc actaacatct gagctaaagt cattgatttt tgtgatatag gtaggcgcat
     9601 aagcaatgaa cgaacccgat agtagaaaaa tcatcacaaa attaatcacg gctcgaactg
     9661 ctttcgtggt ttctcgtttt aataatccgg tatacgccac atacaccccc agagctaaaa
     9721 tcatcaataa tagaaaccca aaataaaatc cggaggtctg gagtccattt tcagtaatac
     9781 cagccaatgt ttggatattc tttccaatac tttcagccgt atccgaaatg aaatctaatt
     9841 tgtaagcctc ttgaaccaca taacccgtgg cgttcgacaa atacaaactc accgtccaaa
     9901 tgaaattagt aatggcataa agaccataca tcacgctttt ccctaagcca tcaccccaat
     9961 tccaaggcaa ccaatcccaa gagctatcca caaagaaatc tagttgatag ttgttcagtg
    10021 aatatttgga atatagattt cctgcttcaa ctgtatcatc aactagccct gtcgcctcgg
    10081 ctaccgtacc aactaaactc aatagaacta tcagagtgag tatgctaata aaaacgattc
    10141 ccacaatccg aaaaagtttc tttttcatcc cctcactcca ttctctcttg aacaggtgga
    10201 cgggtatcaa aggcatggaa gagttcttca aagactggat gtacctgaac cacgcctacc
    10261 cgaccataca aatcctgcat caagcattgg ccattttcca aatctcgtag gcgtttttga
    10321 ttgctttcat cttctcgatc caagccaaag aattccaagg ttttcttgat ttcatgaata
    10381 tcggtactgc gaaacgcaaa cttcaacccg atattattct tcaagcgttc atccagtaaa
    10441 tcatcactat tttgcgtgac aaagtaaacc ccggcattca tagcccgccc agcacgaacg
    10501 agcttattcg ataatgtttt cccttgcgct acttgaagaa aactccacgc ctcatctaag
    10561 tctacaattt tgaagatttc tcgattacta tggataaagt ccaaggcaaa agtggaaatg
    10621 acgattagca tggaaacact aagcaattcc atggtggtat attcttcaaa agtggtttct
    10681 gcatctggta aaaccaaatc cgctacttga atgatattta actgcctatc taaactaatc
    10741 gactgggtaa tcgaaccatc tgagaaaagc aaatgggcaa aatcataatc ggtaaaggac
    10801 tcgatgtgat ccgcgatatt ttccgcaact ggtgagcctt cttttcgaag ctcattaatt
    10861 acttgtaata atccccgttg attgctttgg gtaaccgtcc gaatggcctt acgcaacact
    10921 ggaaacttct ccccatctcg actagaaatt cctgttaaaa aggtcagaat atcaattgct
    10981 agactttcag aatccttctt tttctccata atcacataag gatcaagtaa tccttgattt
    11041 tttggcttac tagttaagtt gagaatattg atttcatccg caatttctgg caaggtttct
    11101 ttccagccac cccgttcccc ttttggatcg acaatcacag cttgtccacc aaaaaggacg
    11161 gcgtaataaa ctaagagatt gttactaaag gactttccgc caccgagtga accgaggaat
    11221 gccgctgcta gcgcattggt aactgaatcc ttgacccctt gtgaggccaa tgccggtttc
    11281 aggtaaacat tgcgccccgt atctacgtta taaccaaagt aaatgccttc caactcccct
    11341 aacatctgag tggcgccaaa accaagtccg gcgagaaaat cagacgtaac atactggata
    11401 taatcattca tgtatcgctt actagaaggt aagaattctt catgtagccc catcatatcg
    11461 ccaaaaggcc ggactagttt tacattggta tcatcataaa aatctagcac ttcatcacac
    11521 cggcgcttta gttcatctac tgattcggca cttactcgaa ccacatagct cagtttgtac
    11581 atggattctt tggactgatc caaggtagtt tccaattcat ctacggaatc caatgcatct
    11641 aagacattgg aatttgtttc attgtcagat tgataggcat ggttgtccaa gtcttttaat
    11701 tcctttttct tattgcgaac ggtagcaagt gcctttttgt tcgtcacaat ctccacattc
    11761 atactagtgt caattgggaa tgtaaattgt tgttgctggt aatagaaaag ttctgaggag
    11821 ggaaactcca tttcccccac aattgtatta attgttaagt acgctacata tgattcatga
    11881 ttctcatgtt ccatgcataa gtaccggggc ttttcttcaa tcaaacaacg actgggacgt
    11941 aataagtcgt aacgtttcac caaagtttcc gacttcagct ttttctttgg tagctgaaat
    12001 tcatattctt caaatggtgt acccttttcg ccatagatat gttcaatcaa atacatcaga
    12061 tcactcggtg tgactcgtct gactttaaat cttcgagcca atttactttc cattagtttt
    12121 tcaagcttgg tatatcggcg aatttcttca ttggataagg aaacaaaatc gcccatcaaa
    12181 tgatgattca ctccatagac aaattcttga aagccggaga agaaagattt cttgagattc
    12241 tttagattga cctcttcatc tgtcgcaatc agtttgaagc caataaaaaa gcgataatca
    12301 atctgactat cgccaatcat ggatactaat gcctctgtct gtaaatcaat tcgttgtttc
    12361 gctacttctg ttaatcgccc ggtaatttcc ttttttgatc gttcttgcgt ggcacgaata
    12421 ctactttctg tggcaatttg taaggcatga atcttgcctt cacgattttg cgcaatcaat
    12481 tgacgaaagt tatcgtggac ttgatatttc tgttcaggtg acaaaaagga gtaattgtaa
    12541 ggtattaact catagtaggc aaaacattct ccctcttgat taaagacaag attattttca
    12601 atatacttaa tcgggtacat agttcacact ccttaccaga gtaatgggtt cgtcccatcg
    12661 tttcttctgt tctttgactt tctttcctgc gaaagtgacc ttaaatcgta agtgatagct
    12721 aataaaagat cgtaaaaatc ccaatggttt cttaccgtca aacgtttttt gcgacatgaa
    12781 ccaagtaact gccaccggta tacctaagta ttttaaaaag gccccctcta tcatagatag
    12841 tggaggcaca tgggcaaata cgatgacaaa cagcaaggat aaaacaaacc aagccatctg
    12901 gttaaaagtc acaggaaaag gcagttgaaa gtcattaatt gcataaatga ccttttcaac
    12961 tgcccaaata ctggtgtagc tttttatttt cttcatcaaa tcattccttt aaaaagaagg
    13021 atagtttaga aatcaactac ccttcaataa ttttgttaat atggatactc aaaaacacct
    13081 cgactcgtta ccaaataatt gcctccaatc tccaagtctc taccacacgc atcaaaatca
    13141 atatacgtct tcaagtgttc tggtacttct ccaaaatcac cagcttctac aatcaagaaa
    13201 tatgctacat cactcatatc atcgcaatct ggataataga taatatcatc cacatgctct
    13261 accatctcct caaaagaatt gaagaaagca tgttgaatct cagaagcaac ttcaccaact
    13321 ggcgttcctt caagttcctc cgctaagcca cacaaccgat taatttcctc aattggcgta
    13381 tactcatcaa tctcaaaggg aagttcataa tcatgaattg catattcctc gtattcgtca
    13441 tttaacccaa ttcgttcttt cacttcatca aaatcgacag gtggggtaaa ccacgccccc
    13501 accaattcac cttcgttata tttcccgagg ttcgcaatat aaacacgcat ctgttccatc
    13561 gtgcttcccc cttacgcacc gacaattcgg ttaaacaaac tgagtaagac atccttcacc
    13621 ccagaagcat taaagaccaa tccgaccgca atcaaagcaa tcaccaaaaa gccaatcagt
    13681 ttggaaaact cccgcttaaa tcctaaaaac aaaccaatga ccacaattgc cagtaagact
    13741 aaggattggg cattggttaa aaaccattga tatatggata ggtaagttcc tgatattata
    13801 aatatctcaa acttttcccc ccattcacac ggagcgtgcg actttcaccg cactccgcgt
    13861 tccatcgtgt ttatattgaa aagtatttat gtagtagacc tatatcaagt ttaattcttg
    13921 attgccagct aatactctat atttatttag tcttatgagc tggactttat ttagcttcaa
    13981 tagagctata tatttttgta ttgtttcttc tgttttagcg tgtattagac ggtgtaccgc
    14041 ctcttttacc aaaataagat ttttataatc gtcatttcca ccagctccag ttggtacaat
    14101 atgatgacaa tgtatttcat tagcttgttg aaattcttcc agagttatag cacattttcc
    14161 atgttgagca gaaaatagcg aaattcgatt gtctgcgtat tctattgtgc gattttccaa
    14221 agataatttc attaactggt acatcagtaa tttatcaaca cttagattgg tgtggataaa
    14281 ttttcttccc tctagagtgt agcaattcgc taaatacacc tttgccattg gttttctgtg
    14341 ttggatatat ccaacaggga aaataggttc gccctcgcca gcaacatacc gaagcatttt
    14401 agatttacca tatcttgttg cctcctttgt cgtaagttgt ctgcctgttt ttctcaatct
    14461 gccccttttg tttgtaccta atctgttagt gaagatagcc attacagctc tattcagttt
    14521 catacaatcc agatttacat tcgttgcaat ctgataataa ttttgcatac ctaataccat
    14581 tgaattataa agtcttgttt cttctaattc cgttttccct tttgagggag aagcaatttt
    14641 ctttgcctgt gataccagct tgtctttttg gtttttgaat tgtttatcac ttatatgtga
    14701 tttaacaact aacttctttc ttctcggtat cattttaatc ttgaaaccaa gaaagtcgga
    14761 gtatcttttt ctaacattta caattcttgt tttttcttgt gatacttcca attttaatcg
    14821 ttgttcaatc cattgagtga ttgctatttt ggctttttca gcactttcct tgtagcggca
    14881 gaaaactcta aaatcatctg cataacgaac catatacatt tcctttaact ttgttttctt
    14941 catttctttg taagcgttgc ttttacatgg gctaccgctt ttattgatat gaaccacata
    15001 gttttttgta acaggatttt cctgccattg gctttctacc caatggtcta actcattaag
    15061 aacaatgttt gctagtagtg gggatattat tcccccttgc ggagttcctt tctctggata
    15121 tgttattgta ccgttagtca ttttgattgg agcttttaat atccttttta aaatgaatat
    15181 caagtgcttg tcccgtattc ccatagccca tatctgtttt atcagcttgg aatggttcac
    15241 gttgtcaaag aaacccttaa tatcaaattc aatcacataa tgtagctggc tgatttgtag
    15301 gcggttatag gttgctttta ttgcattttc aactgaccta ttaggtctaa aaccatagct
    15361 gttttcacta aatttagctt cgcagattgg ttctaatact tgttttatac attgttgtat
    15421 aagcctgtcc cacatacaag gaatacctag aggtctggtt ttaccatttg gttttggtat
    15481 ttcttttcgc ctcactggct taggtcgata cccatgtgga ctgcctgcaa caatgtatct
    15541 tacttttttg acaactgttt ctgctggcaa tttgccaata tctttaatta ttgtaccgtc
    15601 tgttcctgct gttttactgc ctgcatttgc tttgatattt cgatatgcca gtaatatatt
    15661 ttctctcgat aggataatat ccataagatg agtgaaaata tctccattct tacttttggc
    15721 atataaatca tcaaaggtgt tttgcatttc gtaatattca gcgtgtctta aatcttctac
    15781 acacaatgtt tcatttttat ttttaggcat aaggcttcac tcctttactt gaaattgagt
    15841 gagctttttt atcttactcg taatccttat tgaaacaaca taatactttt ctaatatata
    15901 cacgacttga gggcattcct ccattctcat tacaagaatt tcttaggttc gccctctgct
    15961 tttcagcatt agttcaactg cttattgttc ttcgtcagtt tattctcttg atgagaaccc
    16021 aatacctttc ctcgttccga taatcttatc tatacatata tatccttagg tcactagcta
    16081 taagcctgct ggcttgatac cgcctgtaac gttatatggc atttcataac ctatattctt
    16141 actttgccac accagctacg atttctcgta cctttgcatt tctacaaagt tcaaatttag
    16201 acccgtacat tcgctgtttc gtcagttcct tatagaacat tctaaccata gatattttat
    16261 agacccccga cctgttggat acgctaccca cctctggata gttttcgata gaagatatat
    16321 gttcatcaac tattacggta tctttcagcc gtctttaccg agcttcaaac attctgagtt
    16381 tcctcataca atgcttgtcg gaggctcagg gcaggcgttt cagagcgtta cttcatcatt
    16441 ccacctgtca gattatcagt tctcctaata accaaacttt ttcgttcttt tattagtgcc
    16501 ttagcttttc actaaggaac gagtcgcaca taaattttgt ccgaaattca tcttgtcact
    16561 ttcctttctt ttccattgct cgatccgctg ctgcacttgc attcatcaag cacatcacag
    16621 taacaccaag catactgcca ccaaatacca aaaaaatatc ttttacaatt cccatgacga
    16681 ttcctctttt catataatca aattttcaat tgccatactt tgttgctcca aaatcttttt
    16741 atgtcggtct gataagcgtg cattctgtac catatcctgg atataggttg tccgattcaa
    16801 tcgatccaac tccttagcca ttttccacgt aggggcgact tgtcggccta gccaattcaa
    16861 ggttcgttca atggtatacg gttttggctc ggaagttaat tgaatctctt tccgattccg
    16921 tccaataaaa cgcccccatt gttcattcaa gggccagttg gttcgccttt taccttctac
    16981 tttatccgca aatcgcaagt aacgattgat aatggaaaac gtggtgctct caatgctacg
    17041 accttttaac aaatcttgaa tcgcatgaaa agcacggtca tttttcagtc gaatctcaaa
    17101 acgatttttc gtctcagttt ggtctaatgg aatccctaac ttgatatatt gttcatagtc
    17161 tttttcatac aaacagaaat aaacctcact cttgagactg ccgatatata aggtattgcc
    17221 catgccgtcc ttttcatcag cctttaaaag ttccccggaa cgataacttt taaaggtacg
    17281 aaacaaggag atacattctt ccttctgaca ttttttcgtt agctcaggta tatccaatag
    17341 ccccactcga tcattgattg ccaagtccaa gcgtttcatc acgccaccgg cctttagaca
    17401 atcctcgaaa aagtcgtacc agctacgctt ttgagcgagt aaaaaagttt caaattgtcg
    17461 gcaacctcgc ccctttaatt ctaaaagcac acctttatct tcttcatgag acaacatcac
    17521 cacaatatca cccatgacat actgttcttg ataggagtaa aaggcataat cttcatggag
    17581 catatactca aatttcaaat ggagaatttc ttgaataatt tttcgtgcat ccgttgtggg
    17641 aaagcgaatc cttacatagt caaacagtaa cgtcaaaggc aaatcgggat taaagctttc
    17701 cagttggtca aaaattttcc tcatggtttt tatagatgcg gttacgccac cattttctat
    17761 ctgatttaag tacggccgac taagtccaca agccgtagcc aatttatttt gtgaaacgcc
    17821 gtattcgttg cgtcggtctt ttaatagccg ataatctaaa ttccgttgtg ccaagttctc
    17881 acctccaaat tcaaggtgtt aactttttca aatgagttaa cacctataca aattacttac
    17941 tatctttgat acaacaagca ttcttaatgg ttgcttactt attttttgtg tttttgtacc
    18001 cccctgttag atacgggggt gagacatcat ggtcggctaa cgccgtccat gaacaggcac
    18061 agcaccacgg aggatttctg cttcgcgaat cctccgcggt cagcgcctaa cagtcagttg
    18121 tgttggttta ggttactcat ctgtaatacc atttacttcc aactctgctt tcatttctaa
    18181 caagtattga cgaatatctg gttcttttgc ttggtccgca atccgctcaa attctgcaat
    18241 tacttgattt atttcaattc gagcatccgt ctcctcattc attaaatatg gatcgccagc
    18301 gactttgaca ccattcttgc gaatatcaaa ttttaaccgt cgcaattgtt cttctaattc
    18361 tgggtctgac tgaatctttc caatcaccca ataaatttct ccaatcaact catcataaac
    18421 catgtacatc caactctttt aatcttctgg caatatcaaa aatgaccgga actgttacag
    18481 agtttccggc ttgcttatac aactgactat ctgaattgac ttgagaatct cgatcaaaag
    18541 cccaatcggg aaagccctga agtcgccaac actctctagg tgttagcttc cgaatttgat
    18601 actgatccgt gacgatcgct tgttggtctc ctgttagtaa ggtatgaaca ctctgttttc
    18661 ccagacgacc tcggcgactt gtggaattag gaaaagccag attcacactg tcttctggct
    18721 tcacctcttg atattctgtt tcattttttc gaatcatgat ttttgtacca ccttgtttcg
    18781 ttgacaaggt gggggcggtt ccagtaactt cataaactcg a
//
LOCUS       DADVXU010000053        18599 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.055, whole genome shotgun sequence.
ACCESSION   DADVXU010000053 DADVXU010000000
VERSION     DADVXU010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 18599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.055"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            90..1790
                     /locus_tag="KB953_001704"
     CDS             90..1790
                     /locus_tag="KB953_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBB6866680.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            1760..1978
                     /locus_tag="KB953_001705"
     CDS             1760..1978
                     /locus_tag="KB953_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HBB6866681.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            1998..2888
                     /locus_tag="KB953_001706"
     CDS             1998..2888
                     /locus_tag="KB953_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBB6866682.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            2906..3190
                     /locus_tag="KB953_001707"
     CDS             2906..3190
                     /locus_tag="KB953_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866683.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            3677..4291
                     /gene="gmk"
                     /locus_tag="KB953_001708"
     CDS             3677..4291
                     /gene="gmk"
                     /locus_tag="KB953_001708"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBB6866684.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            4296..4601
                     /locus_tag="KB953_001709"
     CDS             4296..4601
                     /locus_tag="KB953_001709"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBB6866685.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            4854..7262
                     /gene="priA"
                     /locus_tag="KB953_001710"
     CDS             4854..7262
                     /gene="priA"
                     /locus_tag="KB953_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBB6866686.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            7277..7768
                     /gene="def"
                     /locus_tag="KB953_001711"
     CDS             7277..7768
                     /gene="def"
                     /locus_tag="KB953_001711"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBB6866687.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            7761..8699
                     /locus_tag="KB953_001712"
     CDS             7761..8699
                     /locus_tag="KB953_001712"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBB6866688.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            8699..10057
                     /gene="rsmB"
                     /locus_tag="KB953_001713"
     CDS             8699..10057
                     /gene="rsmB"
                     /locus_tag="KB953_001713"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBB6866689.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            10070..10810
                     /locus_tag="KB953_001714"
     CDS             10070..10810
                     /locus_tag="KB953_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HBB6866690.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            10807..12876
                     /gene="pknB"
                     /locus_tag="KB953_001715"
     CDS             10807..12876
                     /gene="pknB"
                     /locus_tag="KB953_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HBB6866691.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            13041..13940
                     /gene="rsgA"
                     /locus_tag="KB953_001716"
     CDS             13041..13940
                     /gene="rsgA"
                     /locus_tag="KB953_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBB6866692.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            13953..14603
                     /locus_tag="KB953_001717"
     CDS             13953..14603
                     /locus_tag="KB953_001717"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBB6866693.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            14604..15242
                     /locus_tag="KB953_001718"
     CDS             14604..15242
                     /locus_tag="KB953_001718"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HBB6866694.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            15397..16251
                     /gene="mreC"
                     /locus_tag="KB953_001719"
     CDS             15397..16251
                     /gene="mreC"
                     /locus_tag="KB953_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBB6866695.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            16255..16764
                     /gene="mreD"
                     /locus_tag="KB953_001720"
     CDS             16255..16764
                     /gene="mreD"
                     /locus_tag="KB953_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBB6866696.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            17019..18593
                     /locus_tag="KB953_001721"
     CDS             17019..18593
                     /locus_tag="KB953_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBB6866697.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
BASE COUNT         6301 a         3412 c         4030 g         4856 t
ORIGIN      
        1 ttgttttctt atggaatcta caaagtcgaa tacgagactc atgacctttt tatgacgtac
       61 tggtatactt gaagaaaaag gagagcgcga tggagataaa aaaagtttac ttttcacaga
      121 aaatgtcgct gtattcgcct atccagcaag caaaacaacc ccttccattt tttcaaagtc
      181 atcaagaaag tagagacgct ttttggcgtt ctgcaaaaaa gcaatgtgct tttaaagcag
      241 tatcgaaaaa atataccgtc tattttacta ttgctaaaag tagaggagat gttattttta
      301 ataatctgtt gattgaaggt gaaacttttg aatggaaaaa attttttcgg tatatggaag
      361 cttgcgctcg tttttttatt aaagaaaact gttgtttttt atttcaatct cctctatcag
      421 aagaatggtt gcgtattttt cataaaaacg gctatcaagg aaccacacaa ttaaataaaa
      481 agctggtcta tcatacagca cttgttttag gtggaggagg agcgcacggt gcttatcaga
      541 ttggtgtgtg gcaggcgctg aaggaacatg atatcaactt tgagattatc acaggtacct
      601 cggtaggtgc gttgaacggc gcattgattt tacaaggaga tatgaaaaaa gctgtgaatt
      661 tatggaaaaa gctgtcgaca agacaagtgc ttgcgttacc agaaatggct gcagttgaag
      721 accttcgtga acgcttctat cgagaaagac gccaaatgac gaaaacagca ctaattgaag
      781 gcggggtttc ttctgctcca ttggaaaaga tggtcaaaga caatttggat ttgtccttac
      841 tcaaacataa tcccaaaata cgtttatata cggtgtctac taaactgcca gaactagcgg
      901 aagtagtgac tgatatccaa cagacaaaaa cagaagaaat accagattgg atactcgcct
      961 ctgcttcatt ttatcccgct atggcttatc gaaagatcgg aaaaaataaa tatgcagacg
     1021 gtgggtatcg caacaaaata cctattgata ttgcgattaa caaaggagca acagaggcat
     1081 ttgtcgtaga tgtgcaaggg ccaggcccag cgaaaaaaat tcgtatgcca gacattttta
     1141 tccattggaa atgtcaaaca ttgtggacac taggcagctt tctgcttttt gatagccaaa
     1201 ggaaccaatt aaatttacaa ttaggctatc tggaaatgaa aaaacgatta ggctattact
     1261 atgggaactg gtatacattt cattctgtta aacctgcaag aacatactgg cgaggatttt
     1321 tgtcttactt aacaaaagaa attcagttag aattatcttt tttcaaaagc ataaaatttt
     1381 ggcaaaaact gcgtaattta tataagaaca gggtcgtacc agagacatgc ggattggcga
     1441 tgctagaact tttagcaaaa aaacattttt tactgcccaa tgaaatctat caagtagaca
     1501 tgatgattca aatgatctgc caacaagata taaaaagtat gccggatgat ttattggcac
     1561 aaattggtca attaagtact gaagaatggc gaaggtatcg aaaataccag caaaaaatac
     1621 aaaatgagcg cacgaaaact gtttattttg attatctgct acaaggaaaa cgcaaggatc
     1681 gtttgcagca tgaattgctt aagcaaccag ttgacacatt actgatccta tatttatatt
     1741 atctcaagga ggaacaacca tggcacaaga attttcatat gaaatcgtag aagaaatcgc
     1801 tatattgtca gaaaacaaca aggggtggcg taaagaactg aacctagtca gctggaatgg
     1861 tcgcccgcca aaattcgatc ttcgagattg ggcgccagac catgaaaaaa tggggaaagg
     1921 cctgacattg acgaacgaag agtttgaaca actgcaaaaa gcaatagaaa atatgtaatg
     1981 aggaaggacc ctgtcgaatg aaaagtatga ctggttttgg caaagccatc cgcgaaacaa
     2041 aagcgtatca gttagatatt gaaataaaaa gtgtcaacca gcgttttcta gatgttcaaa
     2101 tacgaagtcc taaaatcctg aactttgttg aaaacgattt tcgtcagctt attaaacagc
     2161 agttaagccg cggtcgaatc gaagtatttg tgaatctagc ttatattggc aaaaacgaaa
     2221 aacagatcgt catcaactgg gaattgattg attcacttat cactcaattg acagacggaa
     2281 ttcaacaacg atacggtagt caaaatcaag tgtcaattag tcggatggcg gaacgcttcg
     2341 tgctggacga aaacttcgta acggtagaag aaaaaccgga agagagcatg gaagaattgg
     2401 cacagttagc tctagataca ttgaaaagcg cacttgattc tattgaggcg agtcgagaaa
     2461 aagaaggtca agctttagct ttagtgttga aaaagaatac tgaagaattc aaagaagtac
     2521 taaaaaaact cgatagtttt gtagaaatct atgaaaagga atttcaaata aggtatcaaa
     2581 aaaagctgga ggaatttctt ggagcgaaag tcgatgagca acggttgttg acggagctag
     2641 ccatcttgtt ggaacgcgga gatattcacg aggaactgga tcgtctggcg atccatgtac
     2701 aaaaactaga tgaacttatc gaagcacaat gtcctgtagg tcgggaatta gatttcctga
     2761 tccaagaaat gaatcgggaa atcaacacga tcggttccaa atcaaatgca atcgaaatta
     2821 aaaatcaagt cgtccagtct aagacgatct tagaaaaaat cagagaacaa gtacaaaata
     2881 ttgaatgacg aaagaggtag agataatgaa atattatgac gtaacgttcc acgaattaag
     2941 tggaaaaact gtggttaaac gggatatccc ttctgaaaaa aacggctttg acgtatggaa
     3001 agatgcctgt gcagactata atgagaacga actgtttatt ttgatcaacg acggtgctta
     3061 cgtgacgatg aatcggaaat ttattgttcg aattgacaca gaagaggtag aagatccgac
     3121 agaaaaagca cgttctcgaa aagatgagat catgggtgta gtgaatactt tgtccaatat
     3181 gggcttttag caaggatgct gaacaaagaa atacaataaa taaacagaat tggatttctt
     3241 cgcttagctt ctttgctcta ggaatacatt taaaggttgt gacaaaattc gtgtcacaat
     3301 ctttttttat tttgaataag tgatgttcaa aaaccatttt ctcggtatta agccaaattt
     3361 atgcaaaaat gtgagaaact attttcacaa aatttttctt catactcgaa actaatcggc
     3421 ttcttcacga cctcttatac acggcgttca aaagcttgca tctgcgataa taaccccaac
     3481 caaacgaaaa acatgagaag ctgtttatgt ttggctgttc ttattacttg atgcaggaca
     3541 gcttttttac gacctcttgg tacagggtag cttttttacc aatttctagc tataattaaa
     3601 gataaccttg aaagttgcag aaaaaaagtg caaaataaac gataagtaca aaaagactag
     3661 ttgaaaggac gtcgtcatgt cagagcgtgg attattaatc gtgttgtctg gtccatccgg
     3721 tgtaggaaaa ggcacagtac gaaaagcaat ttttgagagt gaggataatg attttcagta
     3781 ttcgatctca atgactaccc gtaaaatgcg tgaaggggag gtcgaaggcg ttgattattt
     3841 cttccgaagc aaagaagagt ttgaatcaat gatcgaagct ggagagatgc tggaatacgc
     3901 agaatatgtc gggaattatt atggaactcc tttgtcatat gtcaacaaga cattggatga
     3961 aggaaaagat gttttcttgg agattgaagt ccaaggagca aaacaagtca aagaaaaagt
     4021 gcccgatggt gtattcatct ttttgactcc gccagattta gcggaattaa aatcacggat
     4081 cgtaggccga ggaacagatg cagatgaagt aatcgaaaaa cgaatgaaag ttgcaaaaga
     4141 agaaatcgaa atgatggctc tttacgacta tgctgttgta aatgatgaag tgccgctagc
     4201 agttgaacga atcaaaaaca ttattgccag tgaacatttt cgagtagatc gcgtaatcgg
     4261 taaatatatt aaaatgttaa aggagatgta gtcttatgat gttaaaacct tcaattgact
     4321 cactattaga tcgtgtaaat tcaaaatatt ctctagtgat tttagcaagt aaacgcgcgc
     4381 atgaattgga tgcaagtgca cagcctactc tagattcttt tgattcagta aaaagcgtcg
     4441 gtcaagcttt agaagaaatc gatgcaggaa atgtagtcaa tgatccacat cctgaattaa
     4501 aacgtgaacg tttgaagatg gaagaagagg aacgtcaagc acaaaaagag cgtgaacaac
     4561 acgaattgga atcacgtatc cgtgaagagc aaaaaatgta ataaagaaaa atagagcgaa
     4621 catctaaaaa gaaaagcaaa cccagctgtt tccttttcaa acattcgcca cttattgtct
     4681 ggtagaaaag gctgtgtctg aactttttga cacagttctt ttttttctta tctgctgtta
     4741 attttttgtt aggatttggc agaagataag acaaatccat cgatctctag taaaataggc
     4801 aaggaacttt ttgtcgcttg ataagaaaag acaaaaaaag gaggaaacga accatgacat
     4861 taacagctga agtcattgtg gacgtaccta ctatgcagac cgatcagcca ttcacttatt
     4921 tggttccctc tgaaatggaa acagctttgc aagtcgggat gcgagtagaa gtcccttttg
     4981 gtaatggcaa tcgtcatgtc caaggatttg ttatggctat ccaaaaatca gaagaatcag
     5041 ctaatccatc tttgaaggca gttattcgtt tgctagacct tgctccggtg gtcaatgaag
     5101 aattactttc attagctgat tatatgaaaa aaattactta cgcattcaaa atcacttgtc
     5161 tacaaacgat gcttccgagt gtgatgaaag cagaatatga taaattgatc tatcctttgg
     5221 cagacacacc agaagttcag gtattgtttg atcagcgaga agtgatttcc tggaaggaag
     5281 cagaagaaga aggcagcctt tctcaattga ttcgctggcg ccaagagcaa ttagtagata
     5341 tcaaatatga agtccataca cgaaataaag taaaaacgat tcgtttggtc cgttctttgt
     5401 taactgaaaa gcaaatcgaa gaagaatggg caaaattacg gcaaaatgcc aagaaacaaa
     5461 aagaacttct tttgtgttta tctgaactga gccaagaaga accaattgct tatttcaaaa
     5521 ataaaggaat cagtactgct gtcttgaatc aaggaaaaga aaagggctgg ctggagttta
     5581 tagaatcaga acgttatcgc gacccttata aagaccgagt atttgaccaa acgacggcat
     5641 tggaactgaa tgcagaacaa aaaaatgctg tcgaacaaat catcacagca ggtcagcagc
     5701 aaaaagatca ggtctttttg ctggagggaa tcactggcag cgggaaaaca gaagtatatc
     5761 ttcaggcaat cgctgatgtt ttgtcagaaa ataaaacggc tattatgtta gtgcctgaaa
     5821 tcgcattgac tccgcaaatg gtcgagcgat tcaaaggacg cttcggagaa tcagttgcgg
     5881 tcttacatag cggactttcc caaggtgaaa aatatgatga atggcgcaag attgagcggg
     5941 aagaagcgca agtagtagta ggcgcacgat ctgctatttt tgccccgctg aaaaatatcg
     6001 ggctgatcat tatcgatgaa gaacatgaat caagttacaa acaagatgag acaccgcgtt
     6061 atcatgcgag agatctagca atttggcgca gtaaatatca tcattgccca atagttctag
     6121 gaagcgcgac gccctcttta gaatcaagag cacgagctca aaaaggcgtt tatcagttat
     6181 tgcagttaaa ccaccgagcc aaagcagctg cacagcttcc agctattgag atcgtagata
     6241 tgcgtgaaga gtttcagaat catcggacct ctactttttc tgctaatttg caggaaaaaa
     6301 tacaaaatcg attagacaaa aaagaacaaa ctgtcctact gctcaatcgt cgaggatatt
     6361 cgtcttttgt gatgtgtagg gactgtggat ttgttcttcc atgtcctaat tgcgatatct
     6421 ctcttacact gcatatggat acacgttcaa tgcgctgcca ttattgcgga catgaagaac
     6481 ctattcccaa tcgctgtcct aattgtggcg gcaataaaat ccgctactat ggaacaggta
     6541 ctcaaaaagt agaagaggaa ctccgagaac tttatccgca agctaggatt ttgcgtatgg
     6601 atgtagatac cactcgaaga aaaggggcac acgaacagat tttacaaaaa ttcggtgcaa
     6661 aagaagcaga tatattatta gggacacaaa tgattgctaa aggtttggac tttccagaag
     6721 tgacattagt tggggtgttg aatgctgata cctccctgaa tttaccggat tttcgttcta
     6781 gtgagcatac attccaattg cttacccaag tttcaggacg agcagggcga gcggaaaaag
     6841 caggcgaagt cgtgatccaa acattcaatc ctcaacatta cgccatagaa ttggctaaaa
     6901 aacaaaatta tgagcagttt tatcagcagg aaatgcatgt acggcatcgt ggaggctatc
     6961 ctccttatta ctttactgtg aaaataaccg ccagccatcc tgaagaacaa gtagcggcaa
     7021 agaaaatctt tcagattgcc aatcagctaa aagaagtgtt aagccctcaa agcttgctat
     7081 tagggccaac gcccagcatg attttgagag tgaaaaaccg atattattat caactaatca
     7141 tcaaatataa acatgaaccg aatcttcctc atgttttgga tgaaattttg aatggaagcc
     7201 agaaagaaca gcgacaaggt ctttttgtgg caattgacaa cgaaccatta aactttattt
     7261 aagaaagggc caaattatgc gctatcctat attgatccac cctaatgata aattaaaaag
     7321 aactgctcag ccaatcgatg taatcacgga cgagacaatc gctttattag acaatttata
     7381 tgagacaatg attgcgaatg acggaatagg gatcgctgct cctcaagtag gacaaaacaa
     7441 gagaattgct gtgatcgaag tagatgaagg cgagaaattt gaactgatta acccagaaat
     7501 catcgaagca aaaggtgaaa gccttgacgt agaaggttgt ctcagtattc cgcatgtcta
     7561 tggaacggtc aagcgagcag atgaagtgac cgtgcgttat tatgatcgag acggagaaga
     7621 gatagaagtg acggcttttg gctatttagc ccgtgccttc cagcatgaaa tcgatcactt
     7681 agacggtatc ttgttcattg agaaaatgat acagcagatc ccggaagaag aacttgaaga
     7741 atatatggag gaacatcttg atgactaagc tagtatttat gggcacacct gctttttctg
     7801 tgccgattct tgaagggctg atagaatcag gttacgagat ccaggcagtg gtaacacagc
     7861 ccgaccgtcc agttgggcgg aaacgagtaa ttacgccgac accggtaaaa aaagcggctc
     7921 tgaagcacgg tatccgtgtg ctgcagccag agaaaatctc cggttcgcca gaaatggaag
     7981 aaatcattga attggtgcct gatgtgatcg tcactgcggc atttggccaa ttcttgcctg
     8041 aaaagttatt gcaagtacca aaattagggg caatcaatgt ccatgcttct ttacttccta
     8101 aatatcgggg aggtgcgcct gttcattatg ccatcatgaa tggggagaaa gaaactggtg
     8161 taacgatcat ggaaatgatc aaaaaaatgg atgcaggcgg aatctatgcg caagaaagca
     8221 taccaatcac taaacaagat gatgtaggga cgatgtttga aaagcttagc cttttaggaa
     8281 gaaagctatt gctagaaaca ttgcccaata ttttggatgg acaaaaacca gtacctcaag
     8341 acgagtcaga agcaactttt tcgccaaaca tcacacggga acaagaagca atcgattggc
     8401 ataaaactgc agaagaaatt gacaaccaag tacgcggaat gcgtccttgg ccaattgcat
     8461 tcacgaccta tgagggcata cgctggaaat tattatcggt tgaagtattg gcagaaacta
     8521 cagacaaagc acctggtacg atcatccgga aagacaaaaa aaatctatgg attgcttgcg
     8581 gtaaacagac cgtcttagcc atcaatgaat tacagccagc tggtaaagga aaacaggccg
     8641 tacatgattt ccttaacgga agcggtcagc aagttcagat tggtcagcag gtgaaataat
     8701 gaaaaaaatc cctaaaaaaa tgaaaaaatc agtacgatat atggcattac tgacattaga
     8761 gcgagtgaac aagggtggcg cctactcgaa tcttttgttg aatgagatga tcaaacaaag
     8821 cgagctaaat caaaaagatg tgggtttatt tacagaattg atctatggta cgattagcca
     8881 tcagcgacta ttggaatttt acgttgcacc attgatcgct aaagcaaaaa aagtggatga
     8941 ctgggtcaag atgcttcttt atctttctgt ataccaatta gaatttttag ataaagtccc
     9001 tgcacatgct attttaaacg aggcggtcga aatcgcaaaa gtaaaaggaa atcctgggac
     9061 agggaaattt gtaaatggtg tcttgaggaa ttatcagcgt caaggagcac cagatctgca
     9121 aggcattgct gatcctatcg aaagactttc tgtcaaaatc agcctgcctc tatggatgac
     9181 gaaacgactg gtggatcaaa tcggatacga agaaactgaa aagctcggtt tatcgctata
     9241 tcagcctagc catgctagtg cccgagtaga tacacgccga ctttccagag atcaggccat
     9301 cactgttttg caagaagaag agatcacagc tgaaaaaagc caagtcagtc catacggtat
     9361 agtagcgaaa aaagggcatt tagcttctag ctcattgttc cacgatggcg tcatgacgat
     9421 ccaagatgaa agttcgatgc ttgtagcgcc gtctatgcaa atcgagcctg aacatcatgt
     9481 cttagatgcc tgtgcagcac ccggaggtaa gacgacgcat attgcttctt ttttagatcc
     9541 gtcatgcggt ggagaagtgg tcgctttaga tgtacacgaa cacaaagtca aactcattga
     9601 agataatgca gcacgtctag gtgtctcttc tgctgtctac gctcaaaaga tggatgctag
     9661 agaagtcagt aaacagttcg aagcagagca gtttgatcgg attttagtag acgcgccttg
     9721 ttcaggtctt gggctgcttc ggcgaaaacc agatattcgt tataaaaaac agccggatga
     9781 attagcgaat ttaccaaaaa tccaattgga aattttagaa agctgcgcac caactttaaa
     9841 gtcttccggt attctgactt atagcacttg tacgatctta aaagaagaaa accaagacgt
     9901 tgtttctgct ttcttggaaa gacaccctga ttttgagagg attgatgttt tgaccaatcc
     9961 tatgctacaa tcatcaatcg aagagaaaat gttgactttg tatccgcatc agttttacac
    10021 agatggattt ttcatttgtt gtttgcgcaa aaagtaatga ggtgaaaaaa tgcagattga
    10081 atatcaatca gatgtaggaa gacgacgtaa cacaaatcaa gactatgcca gtgtatttac
    10141 cagccgttca ggtctcaagc ttgctgtatt agcagacggg atgggtggtc atcgtgccgg
    10201 agacattgcc agtcagatgg cggtaacgaa tctaggcgaa gcttgggaaa aacaagaagc
    10261 tgttgacgat gaaaaaatcg cccaatggtt cattcaatcg attcaagaag aaaatatgct
    10321 gatttatcag cgtggacaag aacagccgga atataacggt atgggaacaa caatcgtagc
    10381 ggccgctctg tcagatgact ggttcacgat tgccaatgtt ggggatagca gagcttattt
    10441 gattcgtgaa gataaaatca tgcaattgac ggaggatcat tctttagtca atgaactagt
    10501 caaatcagga gaaatcagcg aagaaatggc agtgaaccat ccaaggaaaa atattttaac
    10561 acgttctgtc ggcatgcctg gaacagtcga agtagatgtt tctactcatc tttggcaact
    10621 aaaagatcga ctgctgttat gttcagatgg cttgacgaac atgctgtctg aagaagaaat
    10681 aggaacgatc atcaatcaag aaggcccatt gtctcataaa gtaactgagc tgattgatcg
    10741 tgccaatgaa gcaggcggag cggataatat caccgttctt cttatcgaat ataaggagga
    10801 cagtgtatga tcgagttggg caaaaaatta aatggccgtt atcaaatcac tggaaatatc
    10861 ggtagcggtg gtatggcaaa tgtatttttg gcgcacgatt taatcttgga tcgtgatgta
    10921 gcagttaaag tcttacgatt tgactttcaa aatgaccaga ctgcgattcg acgatttcag
    10981 agagaagctt tagcagcaac agaactcgtt catccaaaca ttgttagtgt ttacgatgtt
    11041 ggtgaagaag acaacatgca gtatctggtg atggaatacg tcaaaggaat ggacttgaaa
    11101 cgatatatcc aaacacatta tcctgttcct tatgaaacag cagtaaatat tatgcagcag
    11161 atcctttctg ctatttcttt agctcataac catcaaatca tccaccgaga tttgaaaccc
    11221 caaaacgtac tgatcgataa tgaaggtgtt gtaaaaatca cggatttcgg gattgctatc
    11281 gctttatctg aaacatcgat cacgcagacg aatacgatgc taggatctgt tcattatctg
    11341 tcccctgagc aggccagagg aagtatggca acgaagcagt cagatattta tgctttaggg
    11401 atcatccttt atgagatgtt gacgggtagc gtaccgtttg atggtgaatc tgctgtaaca
    11461 attgctttaa aacattttca agatgatctt ccatctataa aagcacttga tccaaatgtg
    11521 ccacaagcac tggaaaacgt gattttacgg gcaacggcta aagaacctgc agaccgttat
    11581 aagtcagccg aggaaatgtc tgatgatctt tctacagtat tatcaccagc tcgtgcaaac
    11641 gaagaaaaat ggcagcctca tgtgatggat aatgaaacaa aagttatcac accccttacg
    11701 gaagatgcac caatgcctga ttcattcaaa tctatgcctc tgccaaaaga caagccagaa
    11761 aacgaaacct taccagagga ggaagaagtt ctgcctgatc ctaaaaagaa aggacagaaa
    11821 aaatggtggc tgatcctatt agctattctt gctgttttag gaatcggaac agctgtattc
    11881 tttgcttctg gcgggcgagg agaagttgtt gttcctgatg tttctgatct ttctgaagct
    11941 tctgctagag aaacactcgc aaaagcgaaa ttagaagtag caacaaaaac agaagaaatt
    12001 gcagatgaca aaatcgaaga aggtaatgta gtgaagacag acccggcagc gggaacgaca
    12061 gtcaagcaaa aacgcgaagt cactctgtat attagtagcg gcacgaaaaa aatcaaatta
    12121 gatgattaca gtggtaaaag ttatgaagat gccgaagcaa gtttgaaaga attagggttt
    12181 tctgctgact tgattcaaaa agaagaggaa ttcagttcag aggtagaaag cggaatgatc
    12241 atttcgcaat ctccatcaga agatacagaa gtcgatccta aaacagacac gattactttc
    12301 acagtcagca aaggaccaga aacagtgact ttgagtagtc atgtaggaga aaactacgaa
    12361 actgttcgta attggcttgt tacacaagga ttcaatatca ataatattag tgctacttat
    12421 gactatagcg acactgtacc atcaggttcg atcatcagtc aggatccagc ctcgggtgaa
    12481 gttgtagcag aagaaactta catcaatttt gtcgtaagcc aaggaacaga gcctaaattg
    12541 aatgatatct ccggctatac aaaaagcgaa gcacaaagtt atttagctag tgtcggtgca
    12601 gaatatatcg ggcatgagac ttccgaatat tcaaacacgg tggataaaga taaagtgatt
    12661 cgtacagtac caggggcagg aacgacaatt accaaaggaa tggttgtgaa cgtaatttac
    12721 tctaaaggac cagatccagc gtcttctagt tctgaaaaag actcttcaga cagttctagc
    12781 agctcaagta gctccagtag cacaagcaac tcttcaaaag aagaatcttc cagctccaca
    12841 agttcatcag ctagcacctc agaaagttca aaataagtgc tgaataaaat atatcagaag
    12901 ggaatgcgac attgatttgt tgcattctct tttttgtgtt tctatctctt ttgatcaatt
    12961 ttcaagccat atactttgag aagtttgcat ggaataacac ctactttcct cttcgtatgg
    13021 taaaataaac aaaaacggag gtggcactta tcaaaggaca aatcagaaaa gcaatcagcg
    13081 gtttttacta tgtatacgca aacggagaga cctatcaaac aagaggaaga ggaaatttcc
    13141 ggaatcggaa aatcacgccg cttgtcggag atcaggtgat ctttgagagt gaaaatcaaa
    13201 cagacggcta tcttctggaa atcttgccga gaaaaaatca attagttcgc ccaccagtca
    13261 caaatgttga tcttggaatc attgtcacaa gtatcgtaga gcctgcattt tcttataatc
    13321 tacttgaccg ctttctagtt accttggaat acgagtcgat cgaaccgatt atttttttga
    13381 caaagaccga cttgtcagac gacccagaaa aaatcgaaga gatcaagcaa acctatacta
    13441 aaataggcta tccggtgatt gtacccaatc atacgggtga tacgacagaa ctgatccgtt
    13501 acttcccaga acggctatcg gtctttatgg gtcagtctgg agcaggaaaa tcgacgctac
    13561 tgaatcaaat atcaccggaa ctgaatttag caacaggaga gatttctgat gcacttggaa
    13621 gaggacggca tacaacacgt catgtggaac tgattcctat ctatggcggg ttagtagcag
    13681 atacaccagg atttagctcg attgactttt tgacaattga agcaggagaa ctgccgagac
    13741 aatttcctga atttgtagaa gcttctgtcc attgcaaatt tagagagtgc atgcatgcta
    13801 aagaacctgg ctgcgaagta aaacatagag tagaaacagg tggtatcgca cagacacgtt
    13861 acgacaatta tttgcaattc ttacaagaaa tcgaaaaaag aaaaccaatg tatgcaaaaa
    13921 aagaaggtaa acgaaaatag gaggaaacaa gaatgaagat agcaccatcc attttgagtg
    13981 cagattttgc caatttacaa agagacattg agttagtgga aaaaggaggc gcagattaca
    14041 tccacgtaga tgtaatggat ggccgatttg tcccaaatat cacattaggg ccaaatattg
    14101 ttcaggcaat ccgtcctgtt actaaattac cgttagacgt ccatctgatg atcgttgatc
    14161 cagaaaacta cattccagca tttgcaaaag ctggagcaga tatcattact gtccacgtcg
    14221 aagcaactcc tcatattcat cgggcacttc aaatgacaaa aagcttagga gttaaagctg
    14281 gagtagttat caatccagga acaccagtct ccatgatcaa acatgttttg cctttggctg
    14341 atcaagtact agtcatgact gtcaatcctg ggttcggcgg acaaagcttt attgaagaaa
    14401 cagtagaaaa aattacggaa ctcgctgatc tacgtagaga gaacaattgg acttatgcaa
    14461 tcgaagtaga tggcggaatc gttccagaaa cagcaaaaat ctgtaaagat gcaggggcgg
    14521 atgtcttcgt tgcaggatca tatatctatg gagccgaaaa tccagtcgga caaatcgaac
    14581 gattaaaaga ggcattagac taaatgaaaa ttttattagc tgctggaggt aatccgcaaa
    14641 actggccgca gtctctgctg tccaaatacg atgcctatgt cgggatagac cgtggtgcgc
    14701 tgtacctgct tcaagcaggc tacccagtag atctggcagt gggtgatttt gattcattgt
    14761 cgaacgaaga gcagaaggaa gtatttgcaa aagccaaaga gtatacccgt tcccctgcag
    14821 aaaaagacga tacagataca cagttaggta tcttaaaaac atttgaacgt tacccgcaag
    14881 cggaagtcac tttgattgga gctacgggcg gaagattgga tcatctttta gccaatctct
    14941 ggttaggtat ggaacctcgc ttcaaaccct ttgcccatca gcttattatc agagattgtc
    15001 aaaattattt gtcttattat ttgcctggag agcatatagt aagacagata ccagatatga
    15061 agtatttagc ttattgctgc ttgactcccg tctcgaatct tacattaaaa ggcagcaagt
    15121 atgtattgga aaataaagaa gtagaatatc cgacatctta tgcaagtaac gaatttcttt
    15181 cagataccgc agaattttct ttttcagatg gtatgattgc agttattcaa agcaaagatt
    15241 gatccataaa caaataaaaa aatttgtctt tcaggctaaa aatgacagaa tctattattt
    15301 gctaaaaaaa agcttaagag gagcaactta ccttttgttt cacggcattt agtgataaaa
    15361 ttgaacacaa gagtctaatt aattcgaggt gtctttgtga agaagttcaa tcctaataaa
    15421 aatattatta ttaccttggt tattgttatc atcgtggtga cgatactaag tgtgaccgct
    15481 gcgcagcgag cgaatgaagg aaagcccaat ttcttccaat ccatcgttaa tgacagtgtt
    15541 gcaatggttg ataaaacaat ctctgcccca gtacggtggg tacgaaatgg agtagattct
    15601 gtccataatc ttttttcaac ttatgccgaa aatgagcggc taaaagaaaa aatcgatagc
    15661 tatgatgacg tttcacagca aaacaagaat tacgagagag aaatcgaagc gttgaaaaaa
    15721 gaacttgagt tgaatcagac gctgactagt tacgaaaaag taacggcaaa tgtcattact
    15781 cgttcgcctg acagttggca ggacatgctg atcgtagaca agggttctaa agacggtatc
    15841 gaagcaaata tggcagtcat gtcgcaaaaa ggattgattg gccgagtgat cgaggtcaat
    15901 acggcttcgt ctaaaatcga attactgtca tcctctaatg aaagctccaa tcattttcca
    15961 gtacgggtat cttcggctaa tggcgaagcg tttggtttgc ttaaaaacta tgatgaaaag
    16021 ctccatgcct tagtggtgac ccaattaact ggtgatacgg atatcaaaga aggggatgtt
    16081 gtccagacat ccggtcttgg agggaattct ccagctaact tgccgatcgg tacggttatt
    16141 aaaacgaaac cagatagtta tgggctggat cgggaagttt atgtgaaacc ttatgcagaa
    16201 atgtatgacg tgtcagttgt gacgattgtc caacggctag cggaggatta gtccatgttg
    16261 aagaaagaaa caatgaagta ctatctgcca atcgttttgt tctttttgat gttgatagat
    16321 ggtcatttaa caagaatgct aggggagtgg tcgaaaggca cctatatgtc aaatgcccac
    16381 tttctgatat tggcattatt atgttgcagt atggcgtttg aaaaacgtta tttactgatt
    16441 accacgattg ttctcggggc tatctatgac gcttactata ttggcgttat cggtatctat
    16501 gcagtagctc tccctttaat tgtatggttg atgtatgtaa tgaaagacgt tatccatgtc
    16561 aacatcttta ctgaattttt cagtatgatc atctttgtca cgggttatga attgtttacg
    16621 atggtggtcc agttgatttt taaattagca gtagtaaata acacgtattt tattacaagg
    16681 tttttaggac ctacactgct gttgaacatg attatatttg tattattcat ttttcccttt
    16741 aagaaattat tcagtgatga ataatttctt tttttatttt taggtcgaaa gcgaaaaaga
    16801 atcgttgtta tcttaagaaa tgctagactt aacgcggctt tttcatgatc attttctgaa
    16861 acgaacagtt ttgtcatttt taaaagttgt tttgaaacat ttttgtaaca taactattat
    16921 atcgttgaca cactgctatg gtacaatttt tatatcgtta agaaagactt aaatatattt
    16981 agatatttat taaattaagc cagtcggagg aatgaagagt gaaaaagagt ttaatatcag
    17041 cagtaatggt atgttcaatg accttgactg ccgtagcatc accgatcgct gccgcggcgg
    17101 acgattttga ttctcagata caacagcaag atcaaaagat tgcagacttg aaaaatcaac
    17161 aagcagatgc acaatcacaa atcgatgcct tagaaagcca agtttctgaa atcaacacac
    17221 aagcacaaga cctattagca aaacaagata cattgcgtca agaatctgca caattagtaa
    17281 aagacattgc agatttacaa gaacgtattg aaaaacgtga agatacaatc caaaaacaag
    17341 cacgcgaagc acaagtaagc aacacaagct caaactacat cgatgctgta ttgaatgctg
    17401 attcattagc agatgctatt ggacgcgttc aagcaatgac aacgatggta aaagcaaaca
    17461 acgacttgat ggaacaacaa aaacaagata aaaaagctgt agaagacaaa aaagctgaaa
    17521 acgatgctaa actaaaagaa ttagctgaaa accaagctgc tttggaatct caaaaaggcg
    17581 atcttttatc taaacaagct gatttgaacg tactgaaaac ttcattagct gctgaacaag
    17641 caactgctga agacaagaaa gctgacttga accgtcaaaa agcagaagct gaagcagagc
    17701 aagctcgtat tcgtgaacaa caacgtctag cagaacaagc tcgtcaacaa gctgcacaag
    17761 aaaaagctga aaaagaagca cgcgaacaag cagaggctga agcacaagct actcaagctt
    17821 catctactgc acaaagctca gcaacagaag aatcatcagc tacacaatct tcaatgactg
    17881 aagaatcatc atcagctaca caatcttcag caacagaaga atcaacaaca cctgaatctt
    17941 caacagaaga atcaacagcg cctgaatctt cagcaacaga agaatcaaca acagcgcctg
    18001 aatcttcagc aacagaagaa tcaacaacag tgcctgaatc ttcagcaaca gaagaatcaa
    18061 caacagtgcc tgaatcttca acaacagaag aatcaacaac acctgcgcct acaacaccat
    18121 caacagatca aagtgttgat acaggaaacg gcacaggaag tagtactccg gctccaacgc
    18181 caacaccaac acctgaacaa ccaaaacctg taacacctgc tccagctcct tctggaagtg
    18241 tgaatggtgc agcaatcgta gcagaagctt acaaatatat tggtactcca tatgtttggg
    18301 gcggtaaaga tccaagtggc tttgactgct cagggttcac acgctatgtt tacatgcaag
    18361 taactggtcg tgacatcggt ggttggacag ttcctcaaga atcagctgga acaaaaattt
    18421 cagtttctca agcaaaagct ggagacttgt tattctgggg gtcacaaggc ggaacttacc
    18481 acgtagcgat tgctttaggc ggaggacaat atatccatgc tcctcaacca ggcgaaagcg
    18541 taaaagttgg ttcagttcaa tggtttgccc ctgactttgc tgtcagcatg taagaatga
//
LOCUS       DADVXU010000054        17979 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.056, whole genome shotgun sequence.
ACCESSION   DADVXU010000054 DADVXU010000000
VERSION     DADVXU010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.056"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            76..384
                     /locus_tag="KB953_001722"
                     /pseudo
     CDS             76..384
                     /locus_tag="KB953_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318053.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            385..702
                     /locus_tag="KB953_001723"
     CDS             385..702
                     /locus_tag="KB953_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBB6866698.1"
                     /translation="MKEARPRDEIDKLYKTKRWRDLRQVVIARDFGMCQECKRRGRNT
                     RGTIIHHIVEAREDLSLFWSVDNLECICVACHNREHPERSGGKKKPKPKSHIVKMYST
                     PER"
     gene            complement(859..1023)
                     /locus_tag="KB953_001724"
     CDS             complement(859..1023)
                     /locus_tag="KB953_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HBB6866699.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGREVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            1126..1647
                     /locus_tag="KB953_001725"
     CDS             1126..1647
                     /locus_tag="KB953_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HBB6866700.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            1634..2107
                     /locus_tag="KB953_001726"
     CDS             1634..2107
                     /locus_tag="KB953_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003666010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HBB6866701.1"
                     /translation="MTTSNILDMSYTERVDYWQSYLEEQASWGGFLKCPYPELLTTWY
                     AERLIDGSIPASKENIQAAKRHMRDLQRQGTDDFPWIFDEEKGHRPIRYIEKKCKPTE
                     GDFGSFVLQPWQHFIIGSMYGWVHRDTGERRFREALIFVGRKNGKFCRLKIGQNR"
     gene            complement(2114..2272)
                     /locus_tag="KB953_001727"
     CDS             complement(2114..2272)
                     /locus_tag="KB953_001727"
                     /inference="COORDINATES: protein motif:HMM:NF024064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HBB6866702.1"
                     /translation="MDKYGLKNRIRISNAIDKDLYEGLKKMSEETMVPMSKLLDKAIE
                     LLLKESQK"
     gene            2365..3126
                     /locus_tag="KB953_001728"
     CDS             2365..3126
                     /locus_tag="KB953_001728"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBB6866703.1"
                     /translation="MKKYIIYKHLNKINGKIYIGVTNDIGRRWRSGGIEYKPPKNETQ
                     HHRSFWNAIQKYGWENYDHLIIEEDLTMKEAFEKEKFYIELYDSTNKKKGYNIAKGGN
                     GGIIYKVHPKGMLGKKQSKEFSSNHSKWAKNHKNNCMTNGDVVWGVTHEHPKGMLGKH
                     HSKESIMKKKAYSGENAVTSKPIVAIERDGTKREFHSAKLCMAYYSISTCVFYRLLKD
                     GAPYVINPKANYRNKEKILAIEGIMFKHKEDTEVS"
     gene            3346..4665
                     /locus_tag="KB953_001729"
     CDS             3346..4665
                     /locus_tag="KB953_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003666013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBB6866704.1"
                     /translation="MVAYDDEQGANVYVLANARDQASLLFDKAAEMVKQSPALFKKFG
                     KPKRSSINYAPAFSKMEPRASDSRKLDGLNTHFGIFDEIHEFTNYNLINVIKKSRGTR
                     KQPLIVYITTAGYVLDGPLMSYFEQGVDCLEHLEDDIDERTFYYLAKLDSAEEADDPR
                     LWIKANPNICLMNFVGMLDDYVKDKKDPKEYADWITKQFNLFSDIDELSFVDMPTIKR
                     NNKTIDIETLKGKKCVGGFDLSETEDFTAAVLEFPLETGEVFILQHTWIPQARFDRDN
                     NQERIKAWEKVGDLTIIPGDYVNYEYVLNWFVENSKIYDIVKINYDKAKALRLNKELE
                     NAGFETNEIRQGFLSLGGPMQNFKEMLLDGKVIFNNSKLYRWYLSNVKLVMDRNSNWM
                     PSKQSKSRKIDGFAASLNSHAEVLNMLVNPVGTGKVTYYSISDLMNM"
     gene            4681..5901
                     /locus_tag="KB953_001730"
     CDS             4681..5901
                     /locus_tag="KB953_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBB6866705.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDAEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALIKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGGNEGLKVKFASNVDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGSNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTNSSKSVAENG"
     gene            5936..6613
                     /locus_tag="KB953_001731"
     CDS             5936..6613
                     /locus_tag="KB953_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HBB6866706.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGIDCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAETPQEETLLQKKLKA
                     LNGGKNE"
     gene            6610..7806
                     /locus_tag="KB953_001732"
     CDS             6610..7806
                     /locus_tag="KB953_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBB6866707.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRQFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     INPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSNWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIINVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTSIANE
                     VPVTNG"
     gene            7821..8144
                     /locus_tag="KB953_001733"
     CDS             7821..8144
                     /locus_tag="KB953_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HBB6866708.1"
                     /translation="MILDPKMDLDEIKNALKIDTDDDDVEVRRAVQAAIAYIRGAIGN
                     DKPSFYKQENDTVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKARYL
                     LFVEE"
     gene            8148..8483
                     /locus_tag="KB953_001734"
     CDS             8148..8483
                     /locus_tag="KB953_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBB6866709.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVRTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            8480..8875
                     /locus_tag="KB953_001735"
     CDS             8480..8875
                     /locus_tag="KB953_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866710.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEKLQQNT
                     PYWDGTKSNGKRGSYMQEHAKDHVVTSSVKNGLIEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            8872..9243
                     /locus_tag="KB953_001736"
     CDS             8872..9243
                     /locus_tag="KB953_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866711.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EIFGNYEDFASNNPLTIQFNVQVDVWVSTLKEVDAFYFALDEIMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            9259..9819
                     /locus_tag="KB953_001737"
     CDS             9259..9819
                     /locus_tag="KB953_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBB6866712.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGVNVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLAKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDERGY
                     VYLTAVESEEMTLQKFKDLVNNKAGE"
     gene            9946..10257
                     /locus_tag="KB953_001738"
     CDS             9946..10257
                     /locus_tag="KB953_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866713.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     R"
     gene            10281..10472
                     /locus_tag="KB953_001739"
     CDS             10281..10472
                     /locus_tag="KB953_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866714.1"
                     /translation="MDQIYKDLIESGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            10472..14317
                     /locus_tag="KB953_001740"
     CDS             10472..14317
                     /locus_tag="KB953_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBB6866715.1"
                     /translation="MAGKGQPAGNIKLGISLDSTNFGNTLDEINAKVKQAESNMRANL
                     KAYDSAGRSYEALSQKTKDLSTVMEGQNAKVRELTKRRDEAISKYGEESKQVANLNTQ
                     INNATAKYNAYSRQLNDTKKELVYSKTAVNDLSNEIKENERQMNAEVKALKAAGDESG
                     AFEAKQKGLAKQTELSEKAIEEQRKVVKLMADEFGDSANETEDAKRALEKLERQSQIS
                     SRQLEALKSSSDQSGKKIEDFGDKSTRSARKLDGLKDKLGSLKSAFSFGAVAGLAHNA
                     ISSVVSGVQGLVGEAVNASDSLMKFSKTMEFANFGKSQIESSKKEMKDYADKTVYGLE
                     EILNTTAQLASNGIPNYTELTKAAGNLNAVAGGSSDTFKSVAMMLTQTAGAGKLTTEN
                     WNQLADAIPGASGLLQDAMLKNGAYTGNFRDAMAQGQITSDEFNQAIVQLGMNDGAVK
                     AATSTDTLSGSWEQMKSTVINGLQSIIDKVGVENITGFINRVTKGIENSIPKITQFIG
                     WLRDIGTWIVENREPLTWIVGIIGGITLAVKALNVASMLLAITGGTLAAPFVAIGVAL
                     GALAGALVIAYTKSETFRNIVNAAFTAVKNVVMSVINNLVEYYKMLWSVLQWLWEKIK
                     EWASWIGNKFIEMKNSVVNTVQNLWNGVKNFFSNGVGDTWNKVVGWVKNIFNKATELK
                     NKVSDAIGNLWNGIKDTFRRGIDTVFNWFSELPTKMKNAIIGGKNAIVDAFKSIFNAA
                     LKAIGKPVNAIIHGASWVLEKLGADKLEEWKVPQYAKGTPNGGHPGGPMMVNDGRGAE
                     AVITPNGQAFIPRGRNVVLNAPKGTHVLTAEETAYMTGNKAPRYRYAKGTGFFGNLWN
                     NVKGFAGDVGNKLKDVVGDVWDFVTDPGALARKVLNGLGVLEGLVKYPLDVGKGILSK
                     ATEALTNKITELFSSGSLDTSMGMQGVYKYLADVAVAVMKKFPGFVATSGYRPGDPYS
                     HGKRNAIDIALPGVTGGSPRYTEAANYAFEKFANKIGYVITNGKVRDRSGQSGTGIHN
                     DWRPWPDGDHYDHVHLNGVKDPQNTQISGDSVGGSGVERWRNVAIRALKMTGQYSTAN
                     LNALLNQMRTESNGNPNAVNNWDINAKNGTPSKGLLQVIDPTFRQYAMPGFNSNIFDP
                     LSNILASIRYALSRYGSLTNAYRGVGYANGGIVNQHQIAEIAEGNKPEIIIPLDKAKR
                     SRAMQLLSIAMDKLGVSPKTYGNATTVSSDYEILEAIEKQAATTNQLLSLLLAFFKGN
                     SRTDRDLALDIQKILVRRM"
     gene            14318..15190
                     /locus_tag="KB953_001741"
     CDS             14318..15190
                     /locus_tag="KB953_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HBB6866716.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSENVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            15187..16236
                     /locus_tag="KB953_001742"
     CDS             15187..16236
                     /locus_tag="KB953_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866717.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGADEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            16240..17064
                     /locus_tag="KB953_001743"
     CDS             16240..17064
                     /locus_tag="KB953_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866718.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVTSTGSTVSISKISDSYELTLS
                     KGEILWSGTALMHDTQTVQPSKKMWQTVSGFLLVWLPYENGQAIGDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            17082..>17979
                     /locus_tag="KB953_001744"
     CDS             17082..>17979
                     /locus_tag="KB953_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HBB6866719.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKTNDRIDQTNQQIGDLGKLKKMYSNSIDFGDYD
                     YSGNPNLSAKLNASSFSLGTGATVADDNDEIVFTLDGTNQLSKYASKAQVPVAEGKQY
                     TISCEIMLEDGFTGDPSGIRLQH"
BASE COUNT         6208 a         3092 c         4051 g         4628 t
ORIGIN      
        1 aagatatgtg caatagccaa acattataaa gttagcttag aatactttca ggatgattat
       61 ttggatagtg atgaacacga ggatattatt cctttgacag acgaaacaag agttaaatca
      121 gtgaaatcgc ttgaacaaag cctaaagtat ctgaataaca aacaagaacc taaccttcta
      181 gagatcaaac taaaagatac tgactcagta ccagaggttt attacaaagg tcagaagtta
      241 gagggattga tagatgtaag ctatcactac catacaacaa ctgatgtggt agctggttca
      301 acaaacgaca tcaatatgga gtacctaggt gaagtaacta agtatccaga tataaaaaca
      361 atcggacaca agagagatgt gtaaatgaaa gaagctagac ctagagacga gatagacaaa
      421 ctatacaaga ccaaacgatg gcgagaccta aggcaagtag taatagctag ggacttcggc
      481 atgtgccaag agtgcaagcg tcgagggcgg aacacaaggg gcacgatcat ccatcacata
      541 gtcgaggcga gggaagacct gtcactgttc tggtccgtag ataaccttga atgtatctgt
      601 gtagcttgtc acaacagaga gcatccagag agatcaggcg ggaagaagaa accaaaacct
      661 aaatcacata tcgttaaaat gtattcaact cctgaaagat aagtttgcag cgaaatgaag
      721 gtagcccccc tactctaaaa gattaaagag taaggcttga gaagaacggt gctgtccttc
      781 cttcgtaaaa agaccgcttt tcaagttttt tggagaaaaa ggaaaaagcc gaccaattta
      841 agccggcttt ggacgaagct atttcttagt ccatttgttt cctttttgag aagtaggagg
      901 taatcggtcg cctggatcaa tttttacttc tcgtccgcct tggacatttc caccacgagg
      961 tcccacttct ttataggttc cttttggttt attgtcttcg ccgggtttat agagttctcc
     1021 cataggaatc cctccctaaa aaaatttcgg cacagcactg ccgataactt aattataagg
     1081 attgtgataa cgatttcaat atatcttttg aaagaaggtg atattatgcc gcaaccagcg
     1141 aagagtgcaa aattacaatt attaaacgga aacccaaata agaagaatac cgaagaactc
     1201 cgcaagcgag cggccgcaga agacaaatta aaaatggcta ctgacaaaat caaaccgccg
     1261 ttatggctag attcgctagg aaaggatacc tttgagttta tcgctgatga attgctgtct
     1321 gtggatttaa tcagtaatcc ggacgtccat acaatggctc tctactccaa ttggtattcg
     1381 caatacgttt ctttagaaaa acagcttcga aaactacaac gagagtacaa gttgaactat
     1441 gcgcttgcga aaaaggaggc agaggcgaga ggtgagccgt ttaatgaacc taatgaatta
     1501 attggtaatc cgctctctcg gcagatggat acagcgtcgc ggaatctccg ttcttttggc
     1561 gctgatttag gactatcacc agcagccaga gctaagttag ctattaagat ggctgatgat
     1621 ggtggtgatg acgatgacga cttctaatat tttggatatg tcctacacag aacgtgtgga
     1681 ctattggcaa agctatcttg aggagcaagc ttcttggggt ggctttttaa aatgtccata
     1741 tccggaattg ttaactactt ggtatgcgga acgattaatc gatggaagca taccagccag
     1801 caaagaaaat attcaagctg ctaaacggca tatgcgtgat ttgcagcgcc aaggaacaga
     1861 tgattttcct tggatctttg acgaagaaaa aggtcaccgg cctattagat atatcgaaaa
     1921 aaaatgtaaa ccaactgaag gcgactttgg ttcgtttgtt ttgcaacctt ggcagcattt
     1981 cataattgga tccatgtacg gatgggtaca tcgtgataca ggagagcgtc gcttccgcga
     2041 ggctcttatt tttgttggac gtaaaaacgg taagttttgc cgtttaaaaa tcgggcaaaa
     2101 tcggtgaaaa cctttatttt tggctttctt ttagtaataa ttcgatagct ttatcaagca
     2161 atttagacat aggaaccata gtttcttccg acattttttt taatccttcg tataaatctt
     2221 tatcaattgc gttagaaatt ctaattctgt tttttagtcc gtatttgtcc atgtgtgaca
     2281 ctccttttta ttttactata acatccgatt ccaccgcttg caactaccat caattgatgg
     2341 tagtatataa aagaggtgag tagattgaaa aaatacatta tttacaaaca cttaaataaa
     2401 ataaatggca aaatttatat cggtgtcaca aatgatattg gcagaaggtg gaggagcggt
     2461 gggattgaat ataaaccacc aaagaacgaa acccaacacc acagaagttt ttggaatgca
     2521 atacaaaaat acgggtggga aaattatgat catttgatta ttgaagaaga cttaacgatg
     2581 aaagaagctt ttgagaaaga aaagttttat attgaacttt atgactcaac taataagaaa
     2641 aaaggttata acatcgcaaa aggcggaaat ggcggaataa tatataaagt tcaccccaaa
     2701 ggaatgctag ggaaaaaaca atctaaagaa ttttcttcaa atcacagtaa atgggcaaag
     2761 aaccataaga ataattgtat gacaaacggt gatgttgtat ggggtgttac tcacgaacac
     2821 ccaaaaggca tgttgggaaa gcaccacagc aaagaatcaa ttatgaaaaa gaaagcatat
     2881 agtggtgaaa atgctgtaac ctcaaaacca attgtcgcaa ttgaacggga tggaactaaa
     2941 agagaatttc acagcgctaa gctttgcatg gcttactatt cgattagtac atgtgttttt
     3001 taccgacttt taaaagatgg cgccccttat gtgataaatc ctaaagcgaa ctacagaaat
     3061 aaagaaaaaa tattagctat cgaaggaatt atgttcaaac ataaagaaga taccgaggta
     3121 agttagtaga ttgcgaaagg ttactaacta ccgtaacgcg taggagttga ataaatataa
     3181 tgctcccaag agtgtccgac agcagataat ttgctgaaaa tgtacgctaa actgggccag
     3241 aactgactga ccgatgaaaa tgagggtgac ctccagagtg cgagataaaa agctcgcaga
     3301 taataacaat cgaaaacatc actaatttca ggcctttcca catacatggt cgcttatgat
     3361 gatgaacaag gcgccaacgt ttacgtattg gcaaatgctc gtgatcaagc aagcttgttg
     3421 tttgataagg ccgcagaaat ggtcaaacaa tcgccggcgc tctttaagaa atttggtaag
     3481 cctaaacgat caagtattaa ttatgctccc gccttttcta aaatggaacc acgcgcctca
     3541 gatagccgga aattggatgg gctaaacact cactttggta tttttgacga gatccacgag
     3601 tttacgaatt acaatctgat caacgttatc aagaaatcaa gaggaaccag aaaacagcct
     3661 ctgatagttt atatcacaac tgctggatat gtattagatg gtccgttgat gtcttatttt
     3721 gagcaaggtg tggactgttt ggaacatttg gaagatgaca tcgatgaacg gactttctat
     3781 tatctggcaa aacttgacag tgcggaagag gctgatgacc caagattatg gatcaaagcc
     3841 aatccgaata tttgtctaat gaattttgtt ggcatgctag atgactatgt taaggataaa
     3901 aaagatccaa aagaatatgc tgactggatt accaagcaat ttaacttgtt ttccgatatc
     3961 gatgagctgt catttgtcga tatgcctacc attaaacgaa acaataaaac catcgatatt
     4021 gaaacgctca aaggtaagaa gtgtgtcggt ggttttgact tgtccgaaac ggaagacttt
     4081 accgcagccg ttttagaatt tccgcttgaa acaggcgagg tattcatttt gcaacacaca
     4141 tggatcccac aagctagatt tgatcgagat aacaatcaag agcgtatcaa agcgtgggag
     4201 aaggtgggag atctaacgat tattcctggt gattacgtca attatgaata cgtcttaaat
     4261 tggtttgtag aaaattcgaa aatctatgac attgtaaaaa tcaattatga caaggccaag
     4321 gcgctacgat taaataaaga actagaaaat gcaggatttg aaaccaacga gattcggcaa
     4381 gggtttctat cattaggtgg gccaatgcaa aacttcaagg aaatgctatt ggacggtaag
     4441 gtgattttca acaattccaa gctttaccga tggtatctat ccaacgtcaa gctggtgatg
     4501 gatcgcaact caaactggat gccgtctaag cagtccaaga gtagaaaaat agatggtttt
     4561 gcagcaagtt tgaacagcca cgccgaagtg ttgaatatgt tggttaatcc tgtcggaacc
     4621 gggaaagtaa cctattactc gatttccgat ttaatgaata tgtaagaaag gtgtggagga
     4681 atgagtattt tagatcgttt gcgttctttt ggccgagcga agccgaaagc gagcaaacaa
     4741 gagtattttt tgaatgaccc gggattgata ccgtatttag tcggaaaaga tgaaatatca
     4801 gaagggattt tttccgtaat tagccgtgta tcgaacgttt ttgcgtctct ccctctcaaa
     4861 atgatagatg cggagtttgg ccaaccggac gactgtcctg catacaactt gttgagcgaa
     4921 ggccctcgat attttacaaa gtttgatttt ttccgggacg tggaagtttt gagaaactac
     4981 caagggaatg cgtatgtgca gattttccga aatatcaatg gagaagtagc agatatggcg
     5041 ttaataaaac ctggtgcttg ccatccagtg attgatatgg atagcgggga gctttactac
     5101 caagtaactg cgactgacaa aggcagttat aagcaagtta tttatgtaca ttacatggaa
     5161 atgctccact ttaaacaacc gaggtttggt ggcttggaag gtacagaccc cacaaaagta
     5221 ttaacgaata ccctcggata tgatcgagaa gtccgaaaaa tctctttaag tcagcttaaa
     5281 ggaggtaatg aagggctaaa agttaagttt gctagcaatg tggatgaaga agctaaaaaa
     5341 gctacagtta aaaacattgc tgatttttat cgacaaaacg gtggactact tgtggaagaa
     5401 aacggtgtag aaatcgaacg tttacaacga gagctggtag acagcaagct tttagatact
     5461 gataaagtat ctcgctccag aatcgcgatg gtctacaacg tgccggaaca tttcattggg
     5521 agtaaccagt cgagttactc ttcacaggaa cagctcaata tggagttttt gacatacaat
     5581 ctagtaccga ccgttaatca atatgaagcg gaactaaata agaaaacact atcgagaacc
     5641 gaaaaagcaa aaggttatcg atataagttt aatgtcgcaa gtttgctaag agctgacact
     5701 caggcaaggg ggcaattcta tcaaatcatg cgacgaggcg gagcatactc agcgaatgat
     5761 gtccgaagat ttgaggactt gcaaccgata aataaaaccg gtatggatga ttatcatatt
     5821 tccggagacc tatatccaat cgatatggat ccaacattaa gaaaaacaaa ctcgtctaaa
     5881 agcgtagccg aaaacggtta ggctttttta gtttgcaccg aagggaggtg aaaggatgaa
     5941 aaaagtgacg ttaagcggcg atgtcgtgga taacgatacc gcgtggttat atgactggtt
     6001 tgggattgat tgtatctcac cagggaaaat ttctgccgct cttacagaag cagcggggga
     6061 tgaagtagaa cttgatatct catctaacgg tggggatgtc ctagcggcaa gcgaaatata
     6121 taccgctatc cgtgcctatc cgggaaaggt atccggaaat gttgtgagta ttgcagcaag
     6181 cgctgcaagt gtaatcgctt gtgcttgcga accgcttaga atctcgccaa cagcacacat
     6241 catgattcac aacgcatggg tgaccactag cggcaacgcc gaggaattaa aagccaatgc
     6301 ggaaatgtta agcagtgtgg atgagtctat cgttaatgct tacgagatca agacaggact
     6361 cgaccggaaa aagcttgctg atttaatggc aaaagatact tggttaaacg cacaaacagc
     6421 agtggcggaa ggttttgcgg atgaaattat gtttgcagaa gcaccagtaa cggtactcaa
     6481 tgcctctcaa ccggttattc caaaaaacgc ggtaactaag ttgaaaaatc taatactcaa
     6541 agcggaaaca ccgcaagaag aaacgctctt gcagaaaaaa ctaaaagcct taaatggagg
     6601 gaaaaacgaa tgaatttaga acaattaaaa aatgcgtggg tcgaggcggg aagtaaagtc
     6661 tctgatttaa acgcgcaact caatgcagca ttggttgacg atgaaaaaac agaagaagat
     6721 gtagtaagtt tgcaagcaca agtaaaagca gcacgggcta aacgggacgg attgaaagag
     6781 caagtggcaa atatggaagc cgaacaagtc ttaaacgtca aaaaagaacc attagataaa
     6841 aaagatgaaa acttgaaaaa caagtttatc aaagacttta aagcgatggt caatggtgat
     6901 cctgctatta tggctacttt gacatctaat acggatgaat ctggtaatgc tatcggattg
     6961 actattcctg tagatgtgca aacgactatt catactttgg ttcgtcagtt tgactctttg
     7021 caagaatacg taaacgttga aaaagtgacc actactagcg gttctcgggt ttatgaaaaa
     7081 tggtctgata ttaacccact gaccgctttg gatactgaag acggtgaaat cccagcaaat
     7141 gatgatcctg cactttactt gatcaaatac ttgatcaaac gctacgcagg tatttctaca
     7201 gtaactaaca gcttgctaaa agatactgcc gaaaacattt tggcatggtt gtctaactgg
     7261 atcgcgaaaa aagtagttgt tactcgcaat acaaaaatct tggcagctat tgatggaatc
     7321 aaagcagcgc aaaagaaaga tgttacagat gttgatggga ttaaagatat cataaacgtc
     7381 caacttgatc cagctatcga agctacatct atgtttatta caaaccaaga tggcttcaat
     7441 gttttagata aagtgaaacg tgctgatgga tcttacttgt tacaaaaaga cgtagcttct
     7501 gcaactggat ataccttctt aggtaaacca atcaagaaaa ttgcttctcg tttcttgcca
     7561 aacaaaggta cgcaagctac tcctaaatat ccactgtata ttggtgatct gaaagaagct
     7621 gttacattgt atgatcgcga aaacatgagc ttgctgacaa cgaatattgg cgggggggct
     7681 tttgaaacag acaccaccaa agtacgggtt atcgatcgat ttgatgtgca gttagtcgat
     7741 gatgaagcag ttgttttagc tacatttaca tctatcgcaa acgaagtgcc agtaacaaac
     7801 ggttaaggag ctgatttctt atgattcttg atcctaaaat ggatttagac gaaatcaaaa
     7861 acgcactaaa gattgatacc gatgatgacg atgtggaagt aagacgtgcg gtgcaagctg
     7921 caattgcata cattagaggg gctatcggga atgataagcc ctctttttat aagcaagaaa
     7981 atgacacggt tgatctgatt aatttagcta ttctgcaatt agcagatcat tattacaacg
     8041 ctcgatcagc aaccgtgagt gggaacttgc gagagtacga tttaggtttt acaagcctaa
     8101 tcttgcaact caaagcaaga tatttgcttt ttgtggagga gtagcgtatg ccccttattc
     8161 aaacaggaaa tttaaatcaa cgcatcaagt ttgtccgaga tacgacggtc aaggacgagg
     8221 acgggcaagt tgtccggact tctacaacca ttcttacttg ctgggcaagt gtgcagacac
     8281 aacgcctgaa cgatattaag acgtccattg gtacggcttt ggaaggaaca ctgacgttca
     8341 ttatccgcta tcaacaaaaa tcagagctaa ccaatgatat gaaagtgcgt tggaatggaa
     8401 aaacgtttga aatcattacg attacgaaag gcgagtttgc gaaggacttc acgacaatca
     8461 ttgcaaaaga ggtttcaaaa tgagtgtaga agtcgatgca accgaagtgt acaaagcgct
     8521 tagggaagta aaagcaaacg ttcaacgagt ggaaagccca gcacttagaa aagctgggga
     8581 gtacgctcaa gaaaagttac aacaaaacac accttactgg gatggaacga agtcaaacgg
     8641 taaacgtggt tcgtatatgc aagaacatgc taaggaccat gtggttacaa gctcggtaaa
     8701 aaacggattg atagaagtcg gctatgataa agatgtttct tggcggatgc actttatcga
     8761 gttcggaaca atcaaacaac gtccaaaagg tttcgtacaa aaaacacaaa agcaaatcga
     8821 aaaacaagta acacaaatca ttgctgacga agtaaaaagg aggctaggac tttgaaaacg
     8881 gcagtatcac aagtctattc aattctgaat agcaatgaaa aaacaaagaa cattgatttt
     8941 tacaccaata gtgttccgga atcggctcaa acagtaccta gccttccagt tggcagaatt
     9001 acagagatat tcggcaacta tgaagatttc gcaagcaaca atcctttgac cattcaattt
     9061 aacgtacagg tagatgtatg ggtatcgacc ttaaaagagg ttgatgcctt ttattttgcc
     9121 cttgatgaga ttatgagggg gaatggttgg caatgcgcat acacggaaca aacagatgat
     9181 gaggatttag aaggtgcaaa gcggattatc aaacgatatg tagcaaatat ttcactaaat
     9241 taaaaggaga gaaaatagat ggcaacagta ggatttgaga gcgtcatttt tggcgtaaaa
     9301 acaggtgcag gcggcactct aaaagaatta gtagcagata agtcgaaagg cggagcgatc
     9361 gaagctaaaa ttactggatt aggcgcaact tctaacacaa catacgcttc aaacgtacca
     9421 ttcttcattg caagtaaagg ggtttcgtcg ccaaaagtta cgcttgacgt ggcagactta
     9481 atggataacg gcatttacag cgaaatcatt ggcgctaaaa ccgtggatgg tgtaaatgta
     9541 attggttcag aaactgaagc gccttacgtg tcggtagtca tggttacagc gaacaaagaa
     9601 ggaaaacgct tattcatggg attggcaaaa ggaaaattca gtcatccaga tatcgacatg
     9661 aaaacagctg aagacaaagg agttgaattg caaaccgatt ctatcgaagg ggaattcatt
     9721 tctgatgaac gtggctatgt atacttaaca gccgtagaat cagaagaaat gaccttacaa
     9781 aaattcaagg acttggtaaa taacaaagcg ggggagtagt taaccctgca tctacaccaa
     9841 cgacagatac agggacacca aaagaaccag aaccaaaaat tgatacacaa ggttagccat
     9901 tttttggcta gccttatttt tttgtaaaaa caaggaggaa aacaaatgat tgaattgcaa
     9961 ttgaaacttg acggaaagaa aaaaacattc aaacaacaag atatttccgc acgtgcaatg
    10021 cgtgagtgta tcaaatttta cgagaaagcg gaaaaagcag acctaactga tttagaagca
    10081 attgattcaa tgattgcaat tacagcagat attttccaag atccagcagt tacatttgat
    10141 gctattttag acggtttgac tgcgagcgag ttagtaccgg cattagaaag tgtttttgaa
    10201 caaatcaatg aactgggaaa caatgaaaaa aagcagacgg cgagcaaaaa gagataagtt
    10261 tttctgaagc taggaaagca atggatcaaa tctacaaaga tttaatcgaa tcaggttgga
    10321 cgatgagaga tgtggacgaa gccgactatc attatttgtt acaccttttt ggagaagtgg
    10381 agagtggcga agaatatgta gatggtgctg atttcatcaa acaattttta tcggctgaag
    10441 acttggtaaa acttgaggaa ggaggtaaat aatggcagga aaaggacaac cggcaggaaa
    10501 tatcaagcta gggattagtt tagatagcac taattttggt aacacgctgg acgaaatcaa
    10561 tgcgaaagtc aaacaagctg agtcgaatat gcgtgccaat ctaaaggctt atgattcagc
    10621 aggacgttca tacgaagcac ttagtcaaaa gacgaaagac ttgtctacgg ttatggaagg
    10681 gcaaaacgcc aaagtaagag aattaacaaa gcgccgtgat gaagcgatta gcaagtatgg
    10741 cgaggaatcg aaacaagttg ctaaccttaa cacacagata aacaatgcta ccgcaaaata
    10801 taatgcttac agtcgccagt tgaatgacac aaaaaaagaa ttggtgtatt ccaaaacagc
    10861 cgtcaatgat ttatctaacg aaatcaaaga aaatgaacga caaatgaacg ccgaagtaaa
    10921 agcgttgaaa gccgctggtg atgaatctgg tgcgtttgaa gcaaaacaaa aagggctagc
    10981 caaacaaacg gaattatccg agaaagctat cgaagaacag cgcaaagttg tgaaactgat
    11041 ggctgatgag tttggcgatt cagcaaatga aaccgaagat gcaaaaaggg cattagaaaa
    11101 gttagaacga caaagccaaa tatctagcag gcaattagaa gcactcaaaa gctccagcga
    11161 tcaatcagga aaaaaaatag aagattttgg cgacaagtcc acaaggtcag ctaggaaact
    11221 ggatgggcta aaagacaaat taggctcgct aaaaagcgca ttttcgtttg gtgcagttgc
    11281 tggattagcg cataacgcta ttagcagtgt agtaagtggc gtgcaaggct tggttggcga
    11341 agcagtaaac gcatcggatt cattgatgaa gttttccaaa accatggagt ttgctaactt
    11401 tgggaagtca cagatagaaa gctcgaaaaa agaaatgaaa gactacgccg ataagacggt
    11461 ttatggttta gaagaaattc tgaacacaac cgcacaattg gcatctaacg ggattcctaa
    11521 ctatacagaa ctaaccaagg cggcaggtaa cttgaatgcc gttgcaggcg gttctagtga
    11581 tacattcaaa tccgttgcca tgatgctaac gcagacggca ggagctggga aactaacaac
    11641 tgaaaactgg aatcaattag cagatgcgat accgggcgct tcagggctgt tacaagacgc
    11701 tatgttgaaa aacggagctt atacaggaaa cttccgtgat gcaatggcgc aaggtcaaat
    11761 cacttctgat gagtttaacc aagcgattgt acagttaggt atgaatgacg gagcagttaa
    11821 agcagctact tccacagata cattgagtgg ttcttgggaa cagatgaaat ctactgtaat
    11881 aaatgggtta caaagtatca tagacaaagt aggcgttgaa aatatcactg gtttcatcaa
    11941 cagagtaaca aaagggattg aaaattctat tcctaaaatt actcaattta taggttggtt
    12001 gagagatatt ggaacgtgga tcgttgaaaa tagagagcca ctaacatgga ttgtcggaat
    12061 cataggcgga attacattag cagtaaaagc attgaacgta gcaagtatgt tgctggcaat
    12121 tactggcgga acattggcag ccccttttgt ggcgattggt gtagcattag gcgcactagc
    12181 aggtgctttg gtgatagctt atacaaaatc tgaaacattt agaaatatag tcaatgcggc
    12241 ttttacagct gtgaaaaacg tagttatgag cgttatcaat aatttggtgg aatactacaa
    12301 aatgttgtgg agcgtgttgc agtggctttg ggaaaaaata aaagaatggg cttcatggat
    12361 tggcaataaa ttcattgaaa tgaagaacag cgttgtgaat acggttcaga acctatggaa
    12421 tggtgtgaaa aacttcttca gtaatggcgt tggagacact tggaataagg tagttggctg
    12481 ggtaaaaaac attttcaaca aagcaactga attgaagaac aaagtttctg atgcaattgg
    12541 taacctgtgg aacggtatca aagacacatt ccgtagaggc attgatacgg tattcaattg
    12601 gttttcagaa ctaccgacga agatgaagaa tgccattatt ggcggtaaaa atgccattgt
    12661 tgatgcgttc aaaagtattt tcaacgcagc acttaaagcg ataggtaaac cagttaacgc
    12721 aatcatccat ggagcttcat gggtactaga aaaactgggt gctgacaaac tcgaagaatg
    12781 gaaagtgcca caatacgcaa aaggaacacc aaacggaggt catccgggcg ggcctatgat
    12841 ggtaaatgac ggtagaggtg ctgaagcggt aatcacacct aacggacaag catttatccc
    12901 acgagggcga aatgtagtgt tgaatgcacc aaaaggcaca cacgttctaa cagctgaaga
    12961 aacagcttat atgactggaa acaaagcacc aagatataga tacgccaaag gtacaggctt
    13021 tttcggaaat ctatggaaca acgtcaaagg atttgctgga gatgttggaa acaagctgaa
    13081 agatgtagtc ggcgatgtat gggattttgt aacagacccg ggagcgttgg ctaggaaagt
    13141 gttaaatggt cttggcgtac tggaagggct tgtcaaatat cctttagatg ttggtaaagg
    13201 tattctaagc aaggctaccg aagcattgac gaacaaaatc acagaactat tcagcagtgg
    13261 cagtttagac acttcaatgg gcatgcaagg cgtttacaaa tacttggcgg acgttgcagt
    13321 tgcagtaatg aagaagtttc caggctttgt ggcaactagt gggtatagac caggtgaccc
    13381 ctattcgcat ggtaaacgta atgccattga tattgcacta cctggtgtca caggaggctc
    13441 gccacgctac acagaagcag ccaattacgc atttgagaag tttgcaaaca aaatcggcta
    13501 tgttatcaca aatggcaagg ttcgtgaccg ttcaggacaa tcaggtacag gtattcacaa
    13561 tgattggaga ccatggcccg atggagatca ctatgatcat gtgcatttaa acggtgtgaa
    13621 agacccacaa aacactcaaa tttcaggaga tagcgtggga ggcagtgggg tagaaagatg
    13681 gcgcaatgta gcaattagag cgttgaaaat gaccggtcaa tacagtactg caaacttaaa
    13741 tgcattacta aatcaaatgc gtacagagtc aaatggtaat cctaatgcag ttaacaattg
    13801 ggatattaac gccaaaaatg gaacaccatc aaaagggttg ctccaagtga ttgacccaac
    13861 attcagacag tatgcaatgc caggattcaa cagcaatatt tttgacccac tatctaacat
    13921 cttagcttca atcagatacg cactatcaag atatggctca ctaacaaatg cctatcgtgg
    13981 agttggttac gcaaacggtg gaattgtaaa ccaacatcaa attgcggaaa tcgcagaagg
    14041 aaacaagcca gaaattatta ttcctttaga taaggctaaa cgatcaagag cgatgcaatt
    14101 attgtctatc gcaatggaca aactaggagt ttctcctaaa acttatggaa atgcgacaac
    14161 tgtttccagt gattacgaaa tcctagaagc cattgaaaaa caggcggcaa caacgaatca
    14221 gctgttatcg ttattgctgg catttttcaa aggaaatagc cgaactgata gagatttagc
    14281 tttagatatt cagaagatct tggttaggag gatgtagatg cgaactgtat tactgagaaa
    14341 tagaataaac gaagaaattg atttgtctac ggaggattat tttgctactg ggttgagtaa
    14401 tatggggttt gaagtaaaaa aagaacatgt gggacagtgg gggaatttca gagaaagcag
    14461 tgagaacgtt gaaatatctg aatttcagtc atctgtaatc atttctgtgc atgggtttcg
    14521 agaaaaagaa ctgtacaatt cacttgtgca gttcctatcg gaaggtccgt ttgaactgga
    14581 atttgctttt gacggcgaaa caatggtaag aagatgcagt ctaaaatctt taagtaagac
    14641 tgaaatcgat ccgaaaacat cgttactaac agacacttta gaactatatt tcacatcaaa
    14701 ctggtattcc gtaaaacgcg aaaaattaat tcagcgaccg aatgtagtga aaacacgtgg
    14761 taaggttttt ccgtataaaa gatcttatat ctacactcaa aacttgtggg agaaaaaagg
    14821 tgtattcaaa ttcaataata attctgtata cctaacgaat agcaaggaac ggatgtcccc
    14881 gctaaaaatt cgtgtgattg ggaagtgttc aaatccgtat tgggaagtaa tccaaaactc
    14941 acaaatcatc gcaacggatg gatacttcat agatatgact gaaacacaga ctctagaagt
    15001 atcaagcctt tttgaagata caacggcgat tttaaaagat attgcagggg tagaatcttc
    15061 tgtctaccaa caacaggatt atacaaaaac taattttgtt caggccccga ctggagaatt
    15121 cagcattgtg tttcatgttg ggggggcaga cgtagagatt gagttatacg aggagcgtga
    15181 tctgttttga ttttagcagt cacgcttttt catcgtgatt taatgcttta taacgaacat
    15241 ttattctcta acagttttga atttggtgcg gacgagatca acgaagaggc tagtagcttt
    15301 acgatggata agtacgttcc tgtaaaaaca ggcgattttc tactagcaaa atatattcct
    15361 agtggaaaat ttgcatattt tggtgtaatt acgtcgcagg aagacgaaaa aatcagttgt
    15421 aaaagcttac ttagtttagc tgatagtgag ataccgactg cacgtgtgtc aggagataac
    15481 tacgaagagc atatccggcg gttgattgaa tactatttgc tgaatgatcc aacgaaacaa
    15541 ctaaaagata ttttagacgt caaagcagaa agtgcgacct ctcattcgta tcaagctacc
    15601 gatacgaata aacacaagtt aagtgcgtat attctcaatg gtttcaaaaa atacaacgta
    15661 aaatggtatt tcaagggaat tcaaaacaga aaaatctata caggcatacg tgctgtaaat
    15721 gaatcaattt acattaaaga caattcttct gaatttagcg attgggatgt gtttgttcag
    15781 gcgccgggcg ctggaaacga gaacaagcta ttaatcgttg ataaagcaat gaaagatata
    15841 gagaaaccga taatactgtc aacatggtat ttggacgaag aaaacaattt gacacaagat
    15901 ggatcgaaag aaaatatcac gaaaccaact gtcaatttgg ttaatatcta cgatcaaacc
    15961 gcagaagata aagcatccta cgaagatgtg gcaaaatcag agttgaaagg caatacgtat
    16021 tcgcatgaaa tcaaagtgaa cgttgtaaga aatgcaaaaa atttgaatgt cgaaacgatt
    16081 gaaacaggga tgtttgccac gatttcttat aaaggaaaga tatacaagtc ggttttgaca
    16141 gcttggcgaa tatcaagtga taaggagttt gtggaattga ctttcggaaa cataagaagt
    16201 cgttttatgg attattttga agataatggg ggataaaaaa tggttagcaa tgtggatgga
    16261 tatcaatttg aaaacgtgaa agtaagcgca gaaaatgatg ctagacttta tcacgtttta
    16321 tataatcgga aaaatcaggt tattgatggt tacgatcagt ctatgaattt atcttcaagc
    16381 ggattaacag taaaagttgc tgcaggagca gcgattattc aaggtcgtat ggtcgttgtt
    16441 cgacaagaag aaagtataac agttccagca aactcaagtg gttatatagc attaacagtg
    16501 gatttgacac aagaagttat acctggatct attcttccag aatcggaaga atatgaatgg
    16561 actaataatc aagtcaagct agaatttata acaaaagtta taaaaggtaa tttaaacaat
    16621 ggtgataagg tctataattt accactatgc tcagttactt ctactggatc aactgtttca
    16681 atctcaaaga tatcggatag ttacgagctg actctctcta aaggagaaat tttgtggagt
    16741 gggactgcgt taatgcatga tactcaaact gtccaacctt caaaaaaaat gtggcagaca
    16801 gtttcaggtt ttttattagt atggctccca tacgaaaacg ggcaagcaat tggggataga
    16861 tatgtaacta cgccttttta taaagagcgt gtaactttta ctaatgtttt aggagaaatt
    16921 gtttcaggat ttgatgacta tcacaaaaag tggtttagta aacgaataaa ctataattcg
    16981 aatactaata tatttacagg tgcagcaagc aatgcaagtg gagataacgc aaatatggtg
    17041 cttaggtata tagtttcttt ttagttagga ggtatggaag agtggcaaat ttagaaatta
    17101 aattatctgc aaataagaga cagccttatc tacgtcaccg tgttgttggt agagttggtg
    17161 atggggggct cacaacaatc aatgtacaac ttcttgagga agatgaaatt acaccttttg
    17221 taattaatct aaacggtact ttgaaatttg tgggcgaagt ttcaaacggt aactataccg
    17281 agggagaacc agaaataatc gattcgacta atgggttaat tagttacacg ttcactaagt
    17341 caaatttcag cacgagtcat caattcaaac aagcatattt tgaatatgta gatcctaacg
    17401 gcaaaaaagt aacttttcag aacttcatca tagacgtgtt agaacgagta gatattaatt
    17461 cggagcaagc gaaatactat atttcttcat tggaaaaatt acagagtgaa atgcaaacca
    17521 ctttcaatca gttcattagt gataaacagg tccaatacga tcaaatctac tcgaaatata
    17581 acgaattagt aagattgata aatgaatcag ataagcaagt aaatgatcgt attgataaga
    17641 caaatgatcg tattgatcaa accaatcagc aaatcggcga tctcggcaag ctgaaaaaga
    17701 tgtacagtaa cagcatcgac ttcggggact atgattatag tgggaatccg aaccttagcg
    17761 ctaaacttaa tgcttcaagt ttttctttag gtactggcgc aacagtcgcg gatgataatg
    17821 atgagattgt gtttacctta gatggcacaa atcaactatc gaagtacgct agcaaagcgc
    17881 aagtgccggt agcagaaggg aaacaatata cgattagttg tgaaattatg ttagaagatg
    17941 ggttcactgg cgacccttca ggaatacgtc tgcaacacg
//
LOCUS       DADVXU010000055        17933 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.057, whole genome shotgun sequence.
ACCESSION   DADVXU010000055 DADVXU010000000
VERSION     DADVXU010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.057"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            80..910
                     /locus_tag="KB953_001745"
     CDS             80..910
                     /locus_tag="KB953_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBB6866720.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(1154..1472)
                     /locus_tag="KB953_001746"
                     /pseudo
     CDS             complement(1154..1472)
                     /locus_tag="KB953_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028215.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1594..1788)
                     /locus_tag="KB953_001747"
     CDS             complement(1594..1788)
                     /locus_tag="KB953_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866721.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(2293..2478)
                     /locus_tag="KB953_001748"
     CDS             complement(2293..2478)
                     /locus_tag="KB953_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866722.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(3328..3669)
                     /locus_tag="KB953_001749"
     CDS             complement(3328..3669)
                     /locus_tag="KB953_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBB6866723.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(3669..3899)
                     /locus_tag="KB953_001750"
     CDS             complement(3669..3899)
                     /locus_tag="KB953_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBB6866724.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            4186..4524
                     /locus_tag="KB953_001751"
     CDS             4186..4524
                     /locus_tag="KB953_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866725.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(4729..4968)
                     /locus_tag="KB953_001752"
     CDS             complement(4729..4968)
                     /locus_tag="KB953_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HBB6866726.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(4944..5291)
                     /locus_tag="KB953_001753"
     CDS             complement(4944..5291)
                     /locus_tag="KB953_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HBB6866727.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(6023..6604)
                     /locus_tag="KB953_001754"
     CDS             complement(6023..6604)
                     /locus_tag="KB953_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBB6866728.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(6787..7413)
                     /locus_tag="KB953_001755"
     CDS             complement(6787..7413)
                     /locus_tag="KB953_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBB6866729.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(7435..8766)
                     /locus_tag="KB953_001756"
     CDS             complement(7435..8766)
                     /locus_tag="KB953_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HBB6866730.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(8921..9322)
                     /locus_tag="KB953_001757"
     CDS             complement(8921..9322)
                     /locus_tag="KB953_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HBB6866731.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(9408..9614)
                     /locus_tag="KB953_001758"
     CDS             complement(9408..9614)
                     /locus_tag="KB953_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866732.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(9709..11211)
                     /locus_tag="KB953_001759"
     CDS             complement(9709..11211)
                     /locus_tag="KB953_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HBB6866733.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(11229..11684)
                     /gene="argR"
                     /locus_tag="KB953_001760"
     CDS             complement(11229..11684)
                     /gene="argR"
                     /locus_tag="KB953_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HBB6866734.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(11782..12399)
                     /locus_tag="KB953_001761"
     CDS             complement(11782..12399)
                     /locus_tag="KB953_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBB6866735.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            12620..13993
                     /locus_tag="KB953_001762"
     CDS             12620..13993
                     /locus_tag="KB953_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB6866736.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            14068..15180
                     /locus_tag="KB953_001763"
     CDS             14068..15180
                     /locus_tag="KB953_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBB6866737.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(15252..16889)
                     /locus_tag="KB953_001764"
     CDS             complement(15252..16889)
                     /locus_tag="KB953_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HBB6866738.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(17057..17665)
                     /locus_tag="KB953_001765"
     CDS             complement(17057..17665)
                     /locus_tag="KB953_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBB6866739.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         5086 a         3652 c         3132 g         6063 t
ORIGIN      
        1 tcgcgagcgc tttctttgaa ttttttaatc tttcgtagca gaatgagaat gtattaaaaa
       61 tagattagga ggaattttga tgtttagaca ccagaaagaa ttgcagtttg aagcaaaacc
      121 agatcgtccg aatccattaa tcgcgaaata tctgcaggag ctgatcggcg gccaatatgg
      181 cgagatgagt gtagctatgc agtacctgtt ccaaggctgg agctgccgag gagaagaaaa
      241 atataaagat atgctgatgg acattgcaac agaagaaatc ggccacgtgg aaatgttggc
      301 gacaatggtt gctcgtttat tagagggtgc gcctgttgaa gatcaagaag cagcgatgca
      361 aaaagatcca acaatcggtg ccattatctc tggaatgaat ccacagcacg cgatcgtagc
      421 tggcttagga cctcgtcctg ctgacagcgt gggcaatcca tggagcggtg cctatatcat
      481 tgcgagtggc aacttgttag cagactttag agctaattta aatgccgaat cacaaggaag
      541 acttcaagta gcacgtattt acgaaatgat cgatgatcgt ggcgtaaaag atatgctgtc
      601 tgttctcctt gcaagagata gctatcatca aaatctatgg gaatgtgcag tgaaagaatt
      661 agaagaaaaa gaaggttcaa tcttagtccc aagtactttc ccgcgtgaca aagaacgaaa
      721 agacattgct tatgacttct ataacttctc agagggagac caaagtagtg aaggttcttg
      781 ggctagtggc aaagcactgg atggcgaagg cgaatatcaa tggttgaaag aacctaagat
      841 cgaaggcgct gcaccaaaat tagaaccagt aacaccaaaa atgtatggga cacctccatt
      901 caagaaataa atatctaccc gtagaaacac ccgttttttg ttccacacgc tttgtgagca
      961 aaactgacta aagtcattaa tcataaccac ccttcaaaag ggtggttttc tctagagcta
     1021 taagctcaaa tcaccggcca gcgcctaaag acgctggctt tcatatttta tgttcaagcc
     1081 atattgcctt tgccagtttt gcccacctct taaaaggttt atgtactact ccttagccct
     1141 taaaaggatc gtcttactct cgtacactca gcttatctaa cgcactatca tgtttttctt
     1201 gttcttgaat gtactttttg atggtcgctt cattttgtcc tacttttgtc acatagtatc
     1261 cttccaccca aaagtgtcga tacccaaatt tatattttag atttgcacaa tctcttgatg
     1321 atctcctgaa tattggctcg atattgatta taaattattt ttcttctata ctttggtgtg
     1381 aatacaatgt ggtatttaca catctacttt gtacgggcta aactatttcc ctttattgct
     1441 ccacccgcat agcaagtggt tttgtatttc aatcgctcgg cgcgctcaat taggtctaaa
     1501 gacaataaat ctaaaaaccc gctgcttcac tgacagaaac aacgggtttt cagaaaaaaa
     1561 tgaaatgaaa aagatctctt acggcaatct attttatttt tttgattttt tataaatgga
     1621 aaatgcttca agaagtccaa gaaataaagc cgtataaaaa caagaaccta tgaaatcttt
     1681 atttattaga tattcaacag aaatcccgat aacactagcc aacaatatat ttatcagaag
     1741 tgtaaaataa ggatgctttt tatagaatag aactagtgtt gttttcatag actctccttt
     1801 cttcatattt ccaattaatc acaaattcac ggcattacta gaaaaattaa aggggcgcag
     1861 aagtggaaaa atactcttcg actttcccta caaaccaggc catcttgtgt ttaataacta
     1921 taccaaatct aaggtacctc taaatctaaa ttccatttgt ggcggtgacc tccaattttt
     1981 aactcttcta tctcccatcc agtatacata accttgatac ttcgaccact tatatatcga
     2041 acacacaaat acctatccat tgcaggagta ctacctaact tagttgctac ttgcatctaa
     2101 ataacagctg aaaagatagg acttactctt agtaaagtat ctgaactatt aatttttaaa
     2161 ctatcatttt tatcctcaac tacatgcaca tctgttgcaa cttcactttc tgtctcatcc
     2221 agataaatga ttaaggaatc gggaattgca atatcttcag ctgacgctgg aatagttgag
     2281 ctaaattagt catcatatct ttattataaa ataaccaaca gcaaaaaata caatgaaaaa
     2341 caaacttgga actaatgtat cattaataaa tgttgaaaaa ttcagcaact cattactgta
     2401 tgattttagc agcccaacca acatataccc tataaaagct aataagatgg ttaggaatcc
     2461 tttagaattt ttactcattc atagacactt ccttaaataa cttttattat agaaggtatt
     2521 gtaaataata atattgacaa aaagtgactc aaaataaaaa aaaccttaca ttatactgga
     2581 atgaactgta ttgatagtgt acctaaaagc tctactgaca taagatcgtg taatcggtaa
     2641 agtaatttta ttaattatac gggatgtaat caattagaga ataaactaat cataaaaggt
     2701 acttttacat tcataattgt cgctttgaga ttctactttg gttcactatc tgacccaatt
     2761 gcacattttc gatcattaca agtattggtg tcgttagaac aactaaataa aaactagcgg
     2821 tagatttttc agaatctatg ctaatttctg tatggatgtt ccagtgatgg caaatttctc
     2881 ttttcattgt ttttcttgtc gcatgatatg cctttttgtg ttattcagtt aaactataga
     2941 aattctttcc tagacagatc ttgatcccaa attttacgtt tttgagcatc gatatagttt
     3001 aaaatgacat tactcatatc taaagagttt agatttttta ggactaaaac tgtcagttgt
     3061 tctttttctt ctttctgtta aagtattgtc gcgacttttc gtatacgtta agatatgtcg
     3121 tgaaaaaaca agttttgtga cacaaaaaaa cgaatgactt gaggaaatca cctcaatcat
     3181 tcgtttaaaa tgctatatat tctttagttt aagatacgga aattaatgcc atcgaccaca
     3241 ctagtagaat gaacgacatt tacttggcct ttttttaatt tcgttatgtt tagcatataa
     3301 aaatattata gagtttactt acaatgctta aaacatatac tgtataatct gatcaatttt
     3361 agccatgtct tgcaatggta agttttctat ttttttgagt tttcgttcct taaaatcaag
     3421 agattttaat tgtgtaataa ttacttgtcc ttttatatct agctcttcag gtaatgagta
     3481 acgtgtcggt aaattcttta tagtggaagt aattggacat atgaccgaaa acattgtttt
     3541 acggttaaat tcataacgag agacgactag tcctggtcgt cttttttgaa tttcttttcc
     3601 tacggaggga tcaaaatcaa tccatacaat atcacctttt ttaggaatat aaattctttc
     3661 ttctgacact ataaaatttc tctcccttca ggagttaaat cttcccattc gtctttctca
     3721 taatactcgg cctcttcccc accactaaat ggatcttcaa tttttggaac taacgtaatt
     3781 gttccatcat cagaatacac aacgatatat tcttggtttt ctgaaggttt ctttccgtta
     3841 tcagaaggta aagttaccac tacagaactt ccttgtaatc tagattttgt aacaagcatt
     3901 atcatcactc ccttaataaa agtatactat ataacactaa gtaacgcaaa attccccttt
     3961 gtaaactatc tagaaaattg ttttgtttaa agtatatctt attggatata aatagaaata
     4021 aattgtagcc agttcatttt ttctttaact aaaaaaataa tccaagaaac attgaaccta
     4081 tggatccaaa agctacccaa atactatagc taaccccccg ctaccttcaa ttttgcagat
     4141 aaaacgcttt gggagaaaat ttcaaagaca acaggccatc ctatcatgcc cgattataaa
     4201 caaaaagctg gctattcctt ttgctctgac aaatttcaat tgggaaagtt cacgaaacta
     4261 gcaggtgaag aagtttcaac tgttcggatc aaagaatgcc cgcttcaaat agaaacattg
     4321 gttcaacgaa tttcaactag acaagacttt gctattgtag aacgtgagat cgtagctatt
     4381 tttgtagaag aagggatttt acatgatcca actcatatca atgtggataa atggcaacct
     4441 cttatctata aattcagaga atatacaact gccaaccagt cgttaacttt gaattttaga
     4501 ttccaagaat tcaaagagtc ataatgataa acatcgttag gtataaataa aaaagactcg
     4561 cagcacgagt aaaaaacaaa tagaagctat acattagaaa ggtcgactgt gaccctatag
     4621 atgcagtata tgcagcttat gagcatttct tctgttctct ttgtttttgg gatctgtgct
     4681 gaagtctttt tcgttttttg ccgctctttc tttgaaaaat cgattatgtt ataccttctc
     4741 cattactaat tcaggcatct gctgagtaat gatttttcgc gcaaaagaaa tatctgttac
     4801 ccaaggttcg atttgttctt cagtcaagac aagcggcatt cggtcatgga ttggtgcgac
     4861 tgtgcggtta ggttcagtag tcattatgat tgattctgcc attttttcat cctgatggct
     4921 acgataaaaa ccgccaatat acactatttt ttccttattt gaaaacataa atttgtcttt
     4981 ttcttcattc cactcattgg gaaaacacaa cgattttgca aaaatggttg atgaaaggtc
     5041 tttttttctt ctactgtctc tgcacgggca ttaatcatca attgtccttt tttaaatccg
     5101 gtaaatcccc acttcatgat ttccggagtg aggatgtttt cttcctcttt tcttaacgtc
     5161 acaacaagat ttgacggaaa aacttctcca gctgctaatt ctgctgcctg cttcggttgt
     5221 ttcgtctggg cttctcgata ataattctga aggagctctg actctagatc aaaaaaataa
     5281 cgaccacaca tcctaatccc tcccaatctt tttttctatc atacactgca acaagaagtt
     5341 cgcaccaaca aaacgctttc aatttttgta gaactaaaaa agttgtgaca agtgacaaac
     5401 tctccagtga atggtattct cctgccaact cctcggtcac aagtcacaat attcaacaaa
     5461 catgagaagc tgctttttga aattgttcct tgtgactcgg agtgaaacgg caggcttacg
     5521 acctctgggt aaacggtgtt caaaagccag tttctcggta ttaagccaaa tttatgcaaa
     5581 aatgtagaaa gctattttca caaaattttt cttaatactc gaaactaagc gactttttca
     5641 caacctcttc cttaaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaaccaaac
     5701 gaaaaatatg agcagctatt tttatttggc tgttcttatt acttgatgca gggcagcttt
     5761 ttcacaacct ctccactaaa cggtgttcaa tcagctaacc ttcggtatta gctcaaaaaa
     5821 cggcaaaata tgaggaacta ttttgccgtt tgtttctcta atactcaggt caaagcgctg
     5881 atttcacaac ctctaataca cggtgttcca gaagtagctt cttcgaaaaa taagccgaaa
     5941 tttcacaaaa atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca
     6001 cttctgtcac aacctctaat tattaaagaa ccttttgcga agttaacaat ctttctttga
     6061 tcaattgaaa attctgccta agtagtgata gatcctgtgt gactttcaac aataagatac
     6121 ttccctggat ttcagcaatc aacgctttgg cttgacggtc tgcttcttca tcggacagtt
     6181 tttcttcaga aagtgccagg atattttgtt tcgtatactt gacccattga ttgactaatt
     6241 ctagtagcaa cagacggtat tcttcatcca ctgtactcaa ctcaataatc aaattaccta
     6301 caggacagcc atgataagtg atctgtgtct catgatcagc gaaaatccag tccaacattt
     6361 cttctaatgc ttcttctggc tgttttccag catttaagat gtcttcgaac aattctgtct
     6421 gccatacgtc catatgcgca tggatcaccg ctagacaaac ttcttttttt gaagaaaagt
     6481 aataataaaa ttgacctttt cctgtatcag aagctgctag aatatctcgg attgacgttt
     6541 catgatagcc ttttttataa ataagatctt tggtcgcttc caagatcttt tcctttgttt
     6601 tcaatgatat tctccttttt catttctcat agtcctatat tatacagtaa attcatcatt
     6661 ttttcttctt aatcgtttaa aaaagactgt gccaaattat ctgacacagc cctcactata
     6721 aaactgttct catatagcta gatgttcatt gattcaagaa ctgcgaggac aaaagcaatt
     6781 cctatcttat gatagttgct ctaatacttc ctctgttgtc atcacttgat ctgcaataaa
     6841 gccaaagttc gtcaacgctg catccattgc ttcttggcca ggagcaccaa ttgcatctgt
     6901 cgctaccaca acattgaatc cactctctac caattcacgc atatgggaat cgctacacaa
     6961 gtttccgttc attccaccaa gaattactgt gtcgattcca ttttttctca attggagcgc
     7021 caaatcattg ctttctggtc caaagatttt atgaggagag ctgatgatcg tattttcatc
     7081 caaataaggt ttcaattctt cgataaaatc ggctcctgaa ccttcttcca ctgattcata
     7141 ttgacttttt ctagcaaaca tctgctgctc aagcatcata tgttctcctg caccgccaaa
     7201 ttgccattta tggtcatgtg ggtaaaaata atgaggagaa ataaagagtt tgtatccttt
     7261 ttcttttgct tgttttacca tttcaatcat attttcgatc gtattgtatt tttctaagat
     7321 atcttttgtc aaaccaaagc caccgcctgc tggtttcaag aactcgactt gcgggtcagt
     7381 gagaactact gctgtatttt tattattgaa catggtggat tctccattcg ttatttattt
     7441 taatacatca tttagagctt cactcattgt cggatgagaa tagatttgat ctctcaataa
     7501 cgtataagga agttttccat tcatagctag cgaaatcaag ttgatgactt catgagaatc
     7561 ttccgcatag atacttgccc ctagaatcaa gtcagtttca gggtcgacta aaactttgaa
     7621 tacaccttta ggatcttcta atacttgtgc tttagggaca ccgcttgtca tatacttgaa
     7681 taatttgtaa ttgacgcctt gtgctttggc ttgtttttct gttaaaccta cattggataa
     7741 agtcggagtg ataaatacac tgtaaggcac agctttccgg ttattcgttg ttcgtttatt
     7801 ctcgcccttc aactgatcca taatgattcg ataatcatct agagagatat aagtgaactg
     7861 caatccgcct ttgacgtctc caatcgccca aatgttgtct gcatctgtac gaaggtattc
     7921 atccacagcg attgctccgc gatctgttag tttaacatct gtattttcta atccaagtga
     7981 tgctgtactt ggttttcttc cagttgcagc caaaactttt gacgcttgga ttgttacttc
     8041 ttggccatct tctgtaaagg tgacggcagc tgttgattct tggtctgcta cttgatctac
     8101 agatacacct agatggaatt cgattcccgc atttgtcatg tcttctaaaa tcatttgcgc
     8161 gatatcttca tcttcacgtg gaagaaattc agaatgggca tcaagaacga caactttaga
     8221 tccgtattca ttgaacattg atgcaaattc caagccaata tatccagccc cgatgatcac
     8281 taatttttcc ggtaattgtt cctgatccat cgcttgcgta gaatcaatca agtagcgact
     8341 tgtattcaat cctttgattg gtaaaatgat tggttcagaa ccagtattga tgaaaatccg
     8401 ttcgccttca acaatcactt tttcaccatt attcgtcact tcgatttgat ggttagaaag
     8461 aaaacgtgct gtaccgtcca tcactgtccc agttgcctca tcgctgatca tatgataatt
     8521 tttggcgcga agcatgcccg tcaatttttc tttccgagca acagcctctg taaagtcgat
     8581 tcctctttct ccattgaaaa tcaatgattt agaaggaata cagccgatgt tgatacatgt
     8641 tccgccatac atacgtgttg acttttcaac gactaaaacc gattcacctt ttgaagctaa
     8701 cgccttcgct aaagtcttac cgccctttcc aaaaccgatc acaatatttt gatatttttc
     8761 catcattttt ccctcgcttc ttttttacct accgattggt acaaaaatat gataaccgat
     8821 aattgtctat tctccaaatc atctgcttta tttgtcaaaa aaaaccagac aaaaagcggt
     8881 cctttcacgt ttttagtctg gttcgatttc ctttattctt ctaaacaatc gttacttcta
     8941 catcgatatt tcctctagtt gctttggagt aagggcagaa ttcatgcgca tcttctgcaa
     9001 ttttttgggc ttcttcttcg ctgatgccat cgatatgcac ttctaatact acaccgatct
     9061 gatagccgcc atcatctaaa ttataaagag agacacgtgc ttttactgtg gactctgctg
     9121 tcattcctgc ttctttgatc atatgttgta ccgccccatt gaaacaagaa gcataacctg
     9181 ctgcaaataa ttgttctgga tttgttcctt ctgcatggat tcctggagga gtgattttca
     9241 tatgcatcga gccatttggt gcctcgactt ctccattgcg cccgccatgg ttgatcattg
     9301 ctgtttcgta tgcttttttc attgtactgc tcctttcgat tgaccatttg ctacttggct
     9361 ataattatag tgtatgacaa gtaaggcgct ttctacaaaa attctgctta ttcgaatacc
     9421 agaatgccgt cttctactat ttttccaaaa aacaaaaaaa tactactcgt gaacaacagc
     9481 gtcatatttg ttccaaaata aatagcaatc ttttctacat aatcttttat atgattgatt
     9541 ttaagataca tatataccag aaagttgacc ccataaacca atactgttaa aaaaataacc
     9601 atattcgtta ccattttttc ctccttttgc tggtctccct accagcactc gatttaacca
     9661 taccactttt tttgccgtcc aagcaactag cctttcaaaa agactggttt attctgctgt
     9721 taatgattca agatcagaaa aatgtccgaa taattttgct aattttacta acgccaactt
     9781 gttctttccg aattcatcat tttctatctg taatactagt aacggctcat gtaagcttaa
     9841 acggatcaaa aaccagccgt ttccgtatgg ctcttctaga tcatatcgaa taccttcttc
     9901 attttccggg tcgaagttca tccctttttc ttcttccaac agtcgcttca tttcttctaa
     9961 tatcttgttt ccttgttcgc gtggttcatc gctgaacaat tttaatcgga tttcttgtac
    10021 ttctacgggc tgtttcaact ctttgatttg ttcatctaat gtgatacctt ttctttttaa
    10081 ttctggcaaa agcatcagga tctttgcgat gacatatgca ccatcatcca gaaaatagtt
    10141 ttccttgaat gcagcgtgtc cgcttgtttc gatggctagc tgtgtatctt tcccaagacg
    10201 gttaagttct aatgctttat tgattacatt tcgatagccg gaaatataac gaacttggat
    10261 gcctcctttt tcttcaatga atctttgtag atgagaagaa gtaggggaat tcgtcacgat
    10321 tcttgttcct ggatgctctt ttaatacgat tgtacttaat actgcgatca aattatttcg
    10381 attgatcact gttccagaag cactgacaac tgcagaacga tccacatctg tatcgaaaat
    10441 gatcccaaga tctgcttcat ttgctaaaac agcctgcttg atactttcca tcgcttcttt
    10501 attatcagga tttgggatat gattaggaaa tcggccatct ggttccaaaa attgggaacc
    10561 tgttgaatcc gcacctaagc tattcaatac tttttcagca aagaatccac cagcaccatt
    10621 tccggcatca acaatgattt tccaaccatt tagtggtttg ctttctagtg ttccaatccc
    10681 ctttttaatt tttgtgacta aatcttgcgc ataaggtgtc aaaaggtctg ctttttctac
    10741 gcttccagtt tgttcgcttt ttattttttg ctgctgtgat aggataaaag caatatcttc
    10801 tttttctgct cctccttctg aggtaaagaa ttttattcca ttataataat agggaagatg
    10861 gctagcagtc agcataaccg cagcatcgca cgagaactgt tcaaattgag tggacataaa
    10921 catagcaggc gtagtagcta attcgaaatc gatgacatgt atcccttgtt ctttcagact
    10981 ttggatcaac gcctctttca gtgctggtcc gcttatccta ctgtcatgac caatcccaat
    11041 cgtccatttt ttatttggat cggttgattt cttctttttc tttatccagt ttgctagtcc
    11101 ccagccgatc ttttttacca tgtctgtcgt caagtttact tcatatcctt caactggtaa
    11161 tgcgattcct cgaatatccg atccattttg cagcgctttc atatctttca tataaaagtc
    11221 ctcctcattt attccctcat tttttccagc ttttctgcaa atcgctgtgc ttcttcttct
    11281 gagcggcaaa taataatgaa tgtatctaca ccggccatcg ttccgacaac ttcagggtaa
    11341 gccacttcat ctagccaatt cgttaccaca tcggcaccat ttcgttcagt caatacgata
    11401 accatgaact gaacagactg gatacgcaaa acctcgcgtc taattgctgt acgcaaacgt
    11461 tcttcattta aagaaacaga tggctggtta aataatgtat agcgtactat tttgttttca
    11521 ttttgacttt ttactaactt catttctcga atgtctcgag aaatcgttgc ttgagtcaca
    11581 tcggcgcctg cttcatgcag ccgcgcaagc agctcttcct gtgtcccaat aggttgttct
    11641 gtaatgatct ggcggatcag tgcttgtcgc tgtttctttt tcatattaga cacctctcaa
    11701 tgttgattat agcaaaagat aagtaatatt tcttcatatt ttggtatttt ttacttttat
    11761 tttttgtttt cattcaggtt gctaaccttt ttctatttta acacgcaaaa tcgtcttcat
    11821 tttttcaggt gctgattttc ttggatcgct gatgtaaatc tctttgtgtt ctttagaagc
    11881 tctttcatag ccttcttttg cgatgaatgc ttctaatttc tcaaacgttg ctggttcttc
    11941 ggcatagggc ccgacgtgta agatttgggc acaaagacct tcttccatct ttttgaatac
    12001 tacttgttct ttcaagtctt ccggaagttt gctagaacgc tgaatggctt cttgtgctat
    12061 cgcttctgta acaaagtcag gctgtcggat catcaattga taagaaaaat gttctttttt
    12121 catcactggt tcatgcaaat acttttcttg cagtccccat tgtccttcca gtggatagac
    12181 agtatacatc tgataatcag gaatcagcca atttttttta ggcgacatgc gtattgcata
    12241 gctgactgca tatagacagg ctactcttcg tttaaagtct tcctgattcg ggtcgccgat
    12301 ccctctgatg ctaaagaatg attttttagg aacctctagt atttgcgggc tttttttcat
    12361 actatagctc ttgtctttac gccattcaat tttttccatt tttcttcctc cgcgaaattc
    12421 catttagtta tttattatca tacctctttt tgagtgactt ggctaatcgt gttgacacta
    12481 tttttcagtt tgtgctagaa taagtgaagt aaataaatcg tacagataac acgtttcggc
    12541 gtgtcgagag agaggcggtt tctattttta taatagaaat cactctcttt ttttacgaaa
    12601 ttttttagag gtgatgtaaa tgacaaaaca aaaacagctg cttttagtac ctgcagtaat
    12661 tgcaacaggg atgatgtctt ttgcgggcgt attgatcgag acggccatga atgtaacttt
    12721 cccaactctt atcagacagt ttgatttaac gactgcacaa gtccaatggg taacaacgat
    12781 ttatcttttg atgatttcga tcattgttcc gctgtctact tatttgaatc gcaattttag
    12841 tctcaaaaaa ctctttttag cttcaaatct gcttttcttg gcaggtataa tgatcgattt
    12901 cttctctcct accttcatgg tcctattgtt tggtcgattg ctccaaggtg cagcgacagg
    12961 tatcgctctt ccgttgatgt tccatattat cttgacattt gcccccctca atcgacgagg
    13021 cgcgatgatg ggtgtcggta ctttgacaac ggccattgct ccagcaattg gccccactta
    13081 tggaggcatt atgacttcac attttacatg gaatcatatc ttcctgtttc ttgtgcccgt
    13141 tttgtttctt tctttgatta tcggctggtt tgctattccc gagatggcag tacaaaaaag
    13201 tggttatttg gattggataa gcgttttagg aatttgtctt ttgtttagtg gaagtctaac
    13261 atttttgagt acgttgggaa cagtttccag ctggattgct ttaatcatcg gcggtattgg
    13321 ctggttcctt ttttactatc gaagtaaaaa agtggagcat ccactgatcc gactgtctat
    13381 tctttcaaat aaaacattcc gcttgttttt gattggcttc ttagtttatc agtttttatt
    13441 acttggtgta tcttttgtct tgccgaattt ccttcagatc gttcaaggaa gtagtccttt
    13501 tgtcgctgga ttagcgatgc ttcccggcgc agcggttggt gctttgcttt ctcctttttc
    13561 cgggaaaatg ctggatcaat atggtccgaa gaagccaatt tttgctgggc tgattctttc
    13621 tttgattggt tggctagcat tattcctact cataggacac gcttcagtgg gtgtattgat
    13681 tgcttgtcat gtgttttata tgatcggtat cggattatct tacagcaata tgatgaccac
    13741 cggaatgaat gcactgaatg aagaactaca aggagacggg aacgcaatgt tcaatacctt
    13801 gcagcagttt tctggcgctg tggctacttc attagtagcg gtcattatca attacgtcca
    13861 gcatcatact tcccataact atgaagtgtc tactacatta ggatcaaaag cagcattagg
    13921 cgtcttgttg ctgttgcttc tcgtaagctt tgctcgattc gcctattatc tgttctttgc
    13981 taaaaaagcg tagattccat atacggcagg tgttgcatct gccgtttttt tgtgtgaaaa
    14041 tggttgtttg taaaaggagg cttttttatg gaaaaaattg ccattatcgg tggcgggatc
    14101 attggtatga ccctagccag ttacttagat acatcaaaat tcaatgttgc tttatttgat
    14161 gaagggacag gacaggcaac taaagctagc gctgggatta tctccccttg gttgtcgaaa
    14221 cgcagaaaca aacaatggta tcgtctggct aaagacggcg ctgctttttt ccctaaactt
    14281 ataaaagatt tccaacttga tcaaacgatt tacgcacaat cagggacact tttgcttcga
    14341 cctgaaaaag atttagctga attggctgtt cttgccgaga aacgaaaaga agaagcgcca
    14401 gaaattggaa acatcgaatg gctttcacct gaacagacat ctcaagcact tccgttattg
    14461 aaaccagcag tttcactgcg tatcagtggt ggtggtcgtc tggatggaaa agcatattta
    14521 gaacatcttg gtcagcgttt agcacaaaaa caaatggagc agatccaggc aaaagtcact
    14581 ttgcataaga agagcgattc ttggctcatc aagggaccgt ttggagaata cacagctgat
    14641 cgtgtaattt tgacacctgg cccggcactt aagcctttat tacaagaatt aggctatgct
    14701 gcagatatac gtccgcaaaa agggcagctc ttagcatttc agacatctta taagatgagt
    14761 caagactggc ctgttgctat gcttgacgga gaagctgatt tcattccttt catggatgga
    14821 aagatccttt taggtgctac ccatgaaaac gatcaaggct gggatttggc tcctactagc
    14881 gaagcttata agggattgac taagagtaca gaacagttct tgactgatcc agatcagata
    14941 aaaaactggc catacgagtt ccgtgtaggt acccgtgctt atacgtcaga tttttcccca
    15001 tttttcggcg tactgccaga tgatccaagc ttactggctg ctagcggtct aggctcttct
    15061 ggcttgacca caggtcctta tatcggctat ctattagcca acttcttaaa tactggtcat
    15121 tggcctacgg aagattatca aaaggacatc tccacctata ttacgaaaaa tccatcatag
    15181 aaaaacagct ttttcaagta aggatcccga gcgatattgc gcatggatta cttcttgaaa
    15241 aagctgttat tttagttcaa tttctgaatc aaattctcca aactttcttt tgcttcatct
    15301 ggcaagagat cttttcctat ctgagcttca atgaatcgta aacgaatatc aagtcgttgt
    15361 ttcaccagat ttttatagga cgtatcctca aaagaaacag gaaaatctgg tataggtaaa
    15421 tagtggatgt cttcaaatgg accaaacgac ttgaattctg ccgtttgttg cacgattctt
    15481 tcaacgattg acaatgtcgt ttcaggtttg atttttttat catcgaaata tccagtattc
    15541 aggacatagc aggctgtttg ctgtttcaaa aacaactctc gaaaagcttg atagtcttcc
    15601 ctaagaggat agacacggaa aggattagca aatggttcaa tgactagctc atctagctgt
    15661 ctttttgtag tgatattttc cgcgtctgat cgttttgttg ctaatgttgc accaaaagac
    15721 gctgctaaaa ctgggtcatt gatcttgata attggcggaa gcgaatcatc tttcatgatc
    15781 caaaaaacag catcgatctt ttcgctaaga tgatcgactc ggtcgggtgt cgcataacgt
    15841 gatttgactg tccgcccatt tccgtttcgt atatctcctg ttactaggac tttttctcct
    15901 ttacttgtac atgtcacgcc cacattctga caagtaagga aagaagaaat ggccggatca
    15961 tcagacggat agtcttgtgt tttatcaaag taagaaggtt ccaaggcagt agcaaaacca
    16021 ttttttcgag aaataacgaa agcatcgtca tgcaagatcg aaacagagtg accttccgta
    16081 tgttttgcta aggtaatcgt tgattttcca gaaccggaca acccaaatac agccatcgta
    16141 taggtctggt cattactagc atattgtttc atcccaccgt ggcaggcaat aaagccattg
    16201 cgatttccca atgcccaagc aagcgtcaaa gtcgcttttt ttaattctcc aaagtaacgt
    16261 aatcctagca cagccgctgt attatggaga gcatcaaaca aaatgagtcc atttggaaat
    16321 gtttcatgtg tccattcggg atcacaaaag atataaagat ctccctcatc aaaggaaaca
    16381 gacttttgat aagtctctaa gatcttcgaa ttcgctggct gaaaattcag caaataagaa
    16441 tacaaattgt tctcaaatcc ttctggaatc agcaaatgac tttgcacaga aaattcttca
    16501 gataggccaa cgattacttt tgtatggtaa aactggcgct tgcgactttg atacacagct
    16561 tctcttaaaa ttttctcgta tttgctttgt tccttgtcta agtcctcaaa gatccgccga
    16621 gcacttgctg ttcttcccac aatgcttcca tcattgaata ctagtgtcct aacatcagct
    16681 ggtaaatcta gttcttcagc atgtgagatt gttaaatctg tgatgatcgt tcctggactc
    16741 ccttttgcta gttgataagc tgtcttccta tcaaaaactt catgaacatt attaccgtaa
    16801 aatgtcgttt ctacaatcga tcgaatactg gataacagtg gatttccact tgtgatttcg
    16861 tttaattcga gactctcttt tgtactcatg gctcctcctc attccctaaa caaaaaaacg
    16921 ttttctttat tatacattat tcgagttttt atttgtactg ctgcgctgtg ggaagcaaac
    16981 gagttatttt tcacacaaaa aaaccgagaa cttgaataaa caagtttctc ggtttcatca
    17041 ttttcctttt atcggcttag taccagccgt ttgctaacca gaatgtttta gcagcatccc
    17101 atgaaccata acgagatgtt acatattgtt ctgctacacg ttcttgattg gcagctgaat
    17161 agtcgccgtt taagtatgaa gcatctaatt gataacggcc gatgtaacgt ccgttagtag
    17221 ctgtgtatga accgcctgat tctttttgtg cgatccattc ttttgcagag cttgaagttg
    17281 gcgctgttgc agcagctggg gcagcaggag ctacttcaac tgtttcaaca ggtgcaacgt
    17341 atgtatgttc ttgcgttgct gtttcagttg tagatgctgt attttctgtt gttttgtttt
    17401 ctgttgttgc tgaagaatca tttgaagttg ggattgttaa ttgttcacca acataaatgc
    17461 ggttgatgtt tgcgatcgaa tttttttcag caattgcatc gatcattgag ttgtctcctg
    17521 caaatttttg tgagatctta gataatgaat cacctgattt tactgtataa acttcatcag
    17581 cgtgagcatt tgtagttcct acaaacattg cagtagcacc agcagcaaaa gttgtaccta
    17641 aaacaatttt cttaaatgaa ttcatattaa actccttttt ttctccgttt tttattcttt
    17701 cgacgctatt aactttatca tttagaaata tttcataggt aaaagaaaca tgacatttaa
    17761 tgacaaaata acgaattatt taatatttgt aacatttaaa caaatatttt tttgatacaa
    17821 aaccataaaa gccttgttgt ataagcaaag taatcgtttt tctatgtttt tttatttttg
    17881 aatgaaaaac gcagattctg taacattcac tgcaataatc cataaaaaaa agc
//
LOCUS       DADVXU010000056        17728 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.058, whole genome shotgun sequence.
ACCESSION   DADVXU010000056 DADVXU010000000
VERSION     DADVXU010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.058"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(270..2267)
                     /gene="tkt"
                     /locus_tag="KB953_001766"
     CDS             complement(270..2267)
                     /gene="tkt"
                     /locus_tag="KB953_001766"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBB6866740.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2369..2620)
                     /locus_tag="KB953_001767"
     CDS             complement(2369..2620)
                     /locus_tag="KB953_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HBB6866741.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2767..3390
                     /gene="lexA"
                     /locus_tag="KB953_001768"
     CDS             2767..3390
                     /gene="lexA"
                     /locus_tag="KB953_001768"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HBB6866742.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3570..3788
                     /locus_tag="KB953_001769"
     CDS             3570..3788
                     /locus_tag="KB953_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866743.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(4077..4589)
                     /locus_tag="KB953_001770"
     CDS             complement(4077..4589)
                     /locus_tag="KB953_001770"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBB6866744.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4603..6900)
                     /gene="recJ"
                     /locus_tag="KB953_001771"
     CDS             complement(4603..6900)
                     /gene="recJ"
                     /locus_tag="KB953_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBB6866745.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(7042..7485)
                     /locus_tag="KB953_001772"
     CDS             complement(7042..7485)
                     /locus_tag="KB953_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HBB6866746.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7486..8274)
                     /locus_tag="KB953_001773"
     CDS             complement(7486..8274)
                     /locus_tag="KB953_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBB6866747.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8271..9212)
                     /locus_tag="KB953_001774"
     CDS             complement(8271..9212)
                     /locus_tag="KB953_001774"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HBB6866748.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9389..9976)
                     /locus_tag="KB953_001775"
     CDS             complement(9389..9976)
                     /locus_tag="KB953_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HBB6866749.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10197..11504)
                     /gene="obgE"
                     /locus_tag="KB953_001776"
     CDS             complement(10197..11504)
                     /gene="obgE"
                     /locus_tag="KB953_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HBB6866750.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11798..12808
                     /gene="gap"
                     /locus_tag="KB953_001777"
     CDS             11798..12808
                     /gene="gap"
                     /locus_tag="KB953_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBB6866751.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12893..13828)
                     /locus_tag="KB953_001778"
     CDS             complement(12893..13828)
                     /locus_tag="KB953_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBB6866752.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            14014..14973
                     /locus_tag="KB953_001779"
     CDS             14014..14973
                     /locus_tag="KB953_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HBB6866753.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(15059..16153)
                     /locus_tag="KB953_001780"
     CDS             complement(15059..16153)
                     /locus_tag="KB953_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBB6866754.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            16342..17103
                     /locus_tag="KB953_001781"
     CDS             16342..17103
                     /locus_tag="KB953_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBB6866755.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(17218..17658)
                     /gene="fabZ"
                     /locus_tag="KB953_001782"
     CDS             complement(17218..17658)
                     /gene="fabZ"
                     /locus_tag="KB953_001782"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBB6866756.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT         5107 a         3854 c         3100 g         5667 t
ORIGIN      
        1 aaagaaacca tatccctaga aaatcactaa attaccaaac accattagaa gtttttttga
       61 gttacgtaaa tggaaagttt tgtctcgctt aatttgacaa ataatattct caagaaacac
      121 aaactaatcc gaagaattct ttgttttgtc atttaaatgt aacatagccg aaaacgaaaa
      181 gtaatattga tttattcttc taacacataa agaacagctg tctccaactt gcagacagct
      241 gttcttatca gtcaaacaat tgcttggatc tatgatagtt gattgtacgt attcacgaca
      301 ttttctactg taaatccaaa ttcttctaaa attttagtgc caggggcaga tgcaccgaaa
      361 tgatcgattg taatcgttgc gccctctgtt cctacgtagc gttcccatcc aaatgatgca
      421 gcagcttcga tagccacacg tttcttcact gatttaggaa gtacactttc tttatattca
      481 gaagattgtt tttcaaagag gtcaaagctt ggcatagaaa caacagaaac atctttgcct
      541 ttttcagcca attctttttg agctttcaca gccaaatcaa cttcagaacc tgttgcaatc
      601 aaaatacctt ctggtgtttc tccttgtgat ggagaaagaa catacgcgcc ttttttcacc
      661 atttcatctg ctacttcttt tgtagaaggc aatactggaa ggttttgacg actcaataca
      721 aggatagtag gcgcatctgt tgtctccatc gctactttcc aagcagcacg tgtttcattt
      781 ccgtctgccg gacgaatcac ttgaacacca ggcatgcagc gaacagatgc tagttgttca
      841 atcggttcgt gcgttggtcc gtcttctccg acagcaaccg agtcatgtgt taagacgtaa
      901 acaacaggag tatgttggat tgctgctaaa cgaactgctg gtcttagata atcgacaaag
      961 acaaagaatg ttccaccgta gatacgagtt ccgccatgaa gttggatacc attcatagcc
     1021 gaagccattg caaattcgcg gacaccgaac cagatgttgc gtccttcata atgttcagga
     1081 gtgaaatctt tatctgcagc caccattgta ttattggaac ctgacaagtc cgctgaacca
     1141 ccccagaagc ttggtatagc tttcgataat tcttggatga cttctttact tgaaacacgg
     1201 cttgcttgac tgcttccaac ttcataagtt ggcaattctg catcccagtt ttctggtagt
     1261 ttgccatcaa aagcatcttc aaattgttgt gctaattcag gataagcttt tttataattg
     1321 gcaaacattt cattccatgc atcttcagct ttttgtcctt cttctatcat cgtttgatgg
     1381 aagcgggcag ctacttcttc tggaacagtg aaatctggat actcccaacc ataaacttct
     1441 ttagccatct tgatgccatc ttcaccaaga ggagcaccat gaacagcaga tgttccttct
     1501 ttaggagcac catagccgat gactgttttt acttcgatca atgttggttt gtctgtttca
     1561 gcttttgctt cttcaatcgc tttagagatt gcttctaggt catttccgtc tttcactaag
     1621 atatgttgcc agccatatgc ttcatagcga gcaccaacgt tttctgtgaa agcttttgaa
     1681 gttggaccgt ctagtgaaat atcgtttgaa tcgtataaaa cgatcaattt ccctagtttc
     1741 atatgacctg ccatcgaact tgcttcttga gatacccctt ccatcaagtc tccgtcaccg
     1801 catattgcat acgtatagtg atccatcaca ttaaagtttt ctttgttata cgttgctgca
     1861 aggtgcgctt ctgccattgc cataccgaca gccattgcaa tcccttgtcc taatggccct
     1921 gtagtggctt ctacaccatc tgtatgatgt acttcaggat gtcctggtgt tttagaatcc
     1981 cattgacgga attgtttcaa atcatcgatt gtcacttgat agccagctaa atgaagcaag
     2041 ctatacaaca ttgctgaccc atgtcctgcc gaaagaacga aacggtcgcg gtcagcccag
     2101 ttttttgatg tcgttggatt tactttcagg tgttttgtcc ataacgcata tgccataggt
     2161 gctgcgccca tcggcaatcc aggatgtcct gagtttgctt tttggactgc ttcaatactt
     2221 aatgtacgaa ttgtgttaac gccaagttgg tcaattttgt caaacaaaat aacccctcct
     2281 attaatttag gtttttttta cctttatttc ttacactttc attctataag atgtcacacg
     2341 aaaaagcaat ctttttcagt attttttact aaaagttgtc atttcggttg tgtaagcctt
     2401 ttttcttttg gatttctttt aatttttccg gggtcacgtc attgccctca ggatcgacga
     2461 ctttcattcc ttcgatatga tgacgcatac cggaacgaaa agcttccaaa tactctttgc
     2521 gcaagttgat ttgttcttgt tcttcggctt ttgagagacc attttccttc tttttacgtg
     2581 caagttcatt gatccgctct attttttcag gtgacagcat gctcagccct ccttttactc
     2641 tccttcatat tatataaaaa ctgaaaaaaa aacaactaga taaactaaat aaaatacgaa
     2701 cattcgtttg atttagcgct ttttatatgg tactatttat ttatcaagtg aaagaaggtg
     2761 taccttgtgg cccgacaaac tgaatcaaga caaatcgaag tcttacgata tatccatgaa
     2821 caagtaaccc aaaaaggata cccgccgact gtccgtgaaa tcggggaagc tgttcagctt
     2881 tcttctactt ctactgttca cggtcatctt gcacggcttg aaaaaaaagg gtttatccaa
     2941 agagatccga cgaaacctcg tgcaatcgag ttgacaaaac aaggtttaga aaagattggg
     3001 attcgtccaa caactattcc agtacttggc gtcgtaacag ctggagaacc cattttagca
     3061 gtagaggatg cttcagactt ctttccaatc cctcctaatc tttcttccga agaaggcagt
     3121 ctctttatgc tgacaattcg tggagatagt atgatcaatg ctgggatatt ggatggtgac
     3181 aacgtgatcg ttcggaagca agaaactgct caaaatggcg atattgtcat tgcaatgact
     3241 gccgaggacg aagcgacttg caaacgattc tataaagaaa aaaacttttt ccgtctgcag
     3301 cctgaaaacg acttgctcga accaatcatc ttagatcaag tatctatatt aggacgagta
     3361 gtaggacttt atcgtagtca tatctattaa ttgctactaa ttaaaagaaa actttttaaa
     3421 aagagtttga gacgttggtc tcaaactctt ttttcgcccg ggttcttctt ctttcaaatt
     3481 tttatcactt ccctctttac aaaccgaaca aatattcgta tactaaatat acgaacaatt
     3541 gttcgcataa attaaagagg tgagaattca tgaaatcaat gagacgtctt ggcttagtat
     3601 taactgtttt gtttttaggt attattattg gtaatttcgg catacgtcca gctttacttg
     3661 tgtttgttcc gttatttact ttgtggttca tgttgtggga tgaaaagaag taccgtcaat
     3721 ctgagcggcg cagaattcat gaagatcatt tctatcatga gccctactac agatccaggc
     3781 aatcataata aggattgact gctaattatt caaaagacaa gaagttaaac ttttacgggg
     3841 gtgtgacaaa aatcggtcac atcctttttt tcgaataaac ggtgttctaa agccagtttt
     3901 tcggtattaa gtcaaattgc ggaaaaagtg attttttaaa cttctactgg tctttgcttg
     3961 tttccattta taaaaaaata gcgaccctca aaagtcagat agagaatctg acttttgagg
     4021 gtcgctttaa aatcgcttct ttaagaataa catcaagaat agcttctttt ttctgcttaa
     4081 tattccatta atgtaatgat gtcatatcct ttgattttgt cgcgtccatg gagatccatc
     4141 aattcgatca aaaatgcgca gcctacgact acaccgccta attgttcgat cagttcgatc
     4201 gtcgctttga ttgttcctcc agtagctagc aaatcgtcac aaatcagcac acgttgacct
     4261 ggtttaatgg catccttatg caatgtcaat gtatctgttc catattctaa tccataagtc
     4321 acttcgatgg tttctcgagg taacttccct tttttgcgga ctggggcaaa accgactcct
     4381 aactcataag caacaggaca tcctacgatg aatccacggg cttctggccc aactaccata
     4441 tcaattcgtt tttcttttgc ataatttacg atttgtttag tagcttcccg ataagcgtca
     4501 ccatctgcca tcagtgggga aatatctcga aacacaattc cttttgacgg atagtcggga
     4561 atactggcaa tataatcttt taaatccact ctttgtttcc tcctaggttg ttaaccattt
     4621 ctttatggta ggaatatcgc tcaaaagcaa aaattcctca actttgattt tattgagccg
     4681 tcgctggtat aatctgctct cagtcaaagg atgactttct gggctagcga ctttttgtaa
     4741 aacaccgttc tctattgtaa caaatttcaa ttcaaaaaac acctgtatca taaaaattaa
     4801 taatttttca gggatacgca aatattgagc tattgctttt attttatgac gtatatcaat
     4861 tcgttcttga gaatggatca atttaaacaa ttttgcgtac tgttctcttg tgcctacccc
     4921 attcaaatag gcctcatcag gtgaaatccc catcagataa acacgattga atgaataaga
     4981 ccgaaaaact tctttcagat ccgctaaatc atctggacaa tccataacaa ctagagagtt
     5041 tggttttctt ttttcaatta ttgcctttac agaatctagt ccttcgaaaa caatcgcagc
     5101 ttcctggata atctcaggca cgaatttcaa cgtcgaagaa tcaaatgcta aataaaggct
     5161 accttccgac gttcggctct gttcacggaa acgttttgcc cgccaatcaa atacttgcag
     5221 accacccaca gaaaaatcag ataccatcag ctgcggtttc ttacgcccgt tccattcatt
     5281 gatcgaaagc tttcctacca catctacctg attattcagc agttcttctt cttgagtacc
     5341 aaaaccaaaa gcgaccgcat caagctgact cgatgcatcg gaaaggacaa atttcagatg
     5401 ctgttggttt gctccgattc ttttgatatt ttctgcttgg acttgtcgaa ataaaaactg
     5461 aggcaaagga ttatccgtac caaatggcgc caatagtttc agctcatcaa ttcgctctac
     5521 tgttacttca tttggcagca atacttcatc aattctcaac tcaggccctc tcatcaagtc
     5581 aatttgatga tcgactatat attggttcat cttctcctgt agtatagtga cattttcact
     5641 tggcatcgtt aaaccgactg ctgcatgatg tcctccgaag taagtaaata aatctcgcat
     5701 ctggtcaagc atttcaaaaa gattcaaggc ttcaatacta cgtccagatc ctttagctga
     5761 tccatcttct tttaaagtaa ggacaagcgt tggtttaccc gtttcattca ttattttccc
     5821 agcaacaatg cccaaaacgc cttcatgcca tcctcggttt accaatagat ggatatgatt
     5881 ctccttattg accatcgcta atgcttcttg tgttatctgc tctacgattg cttttctttc
     5941 ttcattgatc tcattcaatt tttttgcttg aacggatgct tcctcttcat caaacgtcgc
     6001 aagtaacgag acggcaggac gtggatcgcc catccgaccg attgcattta gtctgggagc
     6061 aatcgaaaaa ccgatcgttg tttcgtctgc gtccctcatt tttacgccgc tttcttcaaa
     6121 tagcgcctga agtcctatcc gttctgaatg atgaatggca tcgattccta atgaaacaag
     6181 gattcgattc tcatcagtca atgaaactaa atcggcgatc gttcctatcg ctactaaatc
     6241 caaaaattct gcaggcggtt cttccaataa tgcagaagct actttgaatg ctactccgac
     6301 tcctgccaaa tctggaaatg gatagtgccc cgctggatga cgagggtgaa caatcgcata
     6361 agcgtctggt aaattctcag gtagttcgtg atgatctgta atgatcacgt ctacaccagc
     6421 ttgctgagcg tatgctaccg catcatttcc tgctaccccg ttatcaacag tgatgatcaa
     6481 ctggattcct tcttcgatct tttcttggta taccgattgg tttggtccat acccatgctc
     6541 aaatcgattt ggcaggaata cttctacttc tgcaccaagt aattccagtg tttctttcat
     6601 taccgttgca ctggtgatcc catctgcgtc ataatcaccg taaacgagga ttttttctcc
     6661 actgattaca gcttcttgga ttcggctgat tgctttatcc atatcgtgta tcagatacgg
     6721 atcatgtaat tcttgttcct ttggatgtaa aaatcggttg atatcctttt cctcatggta
     6781 acctctttgc cacagtaact taccgacaaa tggagataat ttcagttcac ggatcatttg
     6841 gagaaaggat tcagaaggct ctgtctcgct taacttccaa tcaaaatttg cttgtctcac
     6901 tttctcactc ctaacttagt tattatagca aaggaaatca gcaaaaaaaa gcagaaagaa
     6961 agaataaaaa cagatcagat ccgctagaca gatgttcagc aggacctgat cttttgtacg
     7021 accatttatg aaatatccct attagtcaaa ataattgaag ctttgtttgg gcgattgagt
     7081 tttttgttct tccggcgctt tcaattgact gatttgtgcg gttttcgctt gtaactccgc
     7141 ctgataagca gcttccaact ccgcacgttc gtttctaaac tcgcgttgct gttcttcttt
     7201 tgcttcttca attttctttt gaagttctgt ttgatattct gacatttcct gagtcagctg
     7261 tttgatttgc tttttttgct gccatagtgt tgccgaagca gtcaaaaata caatcagtgc
     7321 accaatcagc gcagaaccga tgatcactaa gatcaatggc gcagaaaagg aagtgaagcc
     7381 aaaacttaca ggaacatttt tattgttcaa tacagcaaaa agaacaataa tcaaaactaa
     7441 aacaaatcca agaatcacat tgctttgctt tttcaattcc ttcacctact ttttgttaaa
     7501 aattccgctg gctaaataat caccaatcgt tggaaacagc gtataaaatt ttgatgcaac
     7561 ttctaaaata aacggttgat tgatttcccg ttttggatgt ctcatattgc ggacgatcat
     7621 ttttgccaac ttggcaggtt ctaaaacgat ttgtccgact ttttccaaat agcttccgct
     7681 tggatcagcc ttatcgaaga aatttgtctc gattggacct ggattcactg tcgtaacagc
     7741 tacgccaagt ggtttcaatt ctaatcgcaa agcgttggag aagccaagga ctgcaaattt
     7801 cgtggcagaa taaaccgttg attttgcagt tgccattttt ccagccatag aggcgatatt
     7861 aatgatatga ccttgtcttc tttctgccat atcgatcgca aatttttgtg tcaaaaccat
     7921 cattcctaag atgttgactt caaacatatc gtatgctttt ccaaggtcga atgtaaggaa
     7981 gtcttcgaaa atcccaaaac ccgcattgtt taccagaata tcgacgcgcc cgacttcttc
     8041 catcaccttt tcatataatt cgtccacgct ttcaggattg ctgacgtcta ccttgaaagc
     8101 ataagcctct ttgccgctca agcgtctgca ctcgtctctg acaccttcta caaatgccat
     8161 tcttcgtgcg caacttataa cgattgctcc ttgctttgct gcttcataac agatttgtgc
     8221 gcccagaccg cctgaactac ctgttactaa aacaacttta tcttctaaat tcatgcctct
     8281 tcctcctcgc gaaatggaat ctcaatgcta tcaaaatctt tcataatcgt cgtgttttca
     8341 aacacttctt gtgcttcttt ttcaagctct aaagctgctt ttcctaaata tctcgcactg
     8401 atatgagtaa gtagcagccg ttttgcctgc gcttcttttg caacttcagc agcttgatga
     8461 gtcgtggaat ggaaatagtt gtgcgccatt cttgcttcat ctttattaaa tgtgctttca
     8521 tgtaccagaa tatcgctatt ttccgcaagc acaacggaat tatgtgtttt tctcgtatcg
     8581 cctaaaatag tcacgattcg tcctttttta tctggaccta cgaaatcttt tccattgatc
     8641 gtccgcccat cttctagttg gatcgtttca ccttgcttca gtttgccgta cagtggtcca
     8701 gctggaatac ctaattcttt tagacgatcc acctgcaatt ctcctttatg atcatgttca
     8761 accacacgat aaccaaaact gtcgatccca tgatttagtt tttttgtata aacagaaaat
     8821 tgctgatcag taaaaatcgg ggctgtttca tttagctcaa tgaatttcag cggataactt
     8881 aaacgcgttt gcgaaatacc taaagagact tttatgtatt cctctatccc ttttggcccg
     8941 taaatctcta atggcgtatc cccaccttga aatgaccggc tgctgatgag cccgggaagt
     9001 ccaaaaatat gatcgccgtg taaatgtgta atgaaaattt taccgatttt tctcggacga
     9061 atagtcgttc gcaaaatctg catttgcgtg ccttcgccac agtcgaacaa ccaaacttca
     9121 ttgcgctcgt ctaaaagttt caatgctata cttgtcacat ttctatgttt tgcaggaaca
     9181 cctgcacctg ttcctaaaaa ttgtaattcc ataatctacc tgactttctt taagttcctc
     9241 tttaatttta gggtaaaaag aagggataat caaccgtaag tcgagaatgg gttcaaatta
     9301 aaaataacgt tctaaagcga tagcgttttc tttgaaaatt actataaaaa cagcagcgaa
     9361 cccgtttgag atcctctgct gttttttatt aatttgaaga tccagaactt tcattgtcgt
     9421 tggaagtttg cggatgtttg tccttccaag tcttttgtgc agcttctttc gcatgatttt
     9481 cagcttcttt cgcatcatgc acatcttttt cgacatcttc ataatccaag cgagtaattt
     9541 ctccgcctaa ttccatcatt accaattgat tcagtgggcg attgtcctct tcatcagcaa
     9601 tcaagatcag tcctgtagct ccttcaccga ttttatttcc tacaaagtcg aacacggtct
     9661 gggcatcttt tatttctttg gcatcttgtg tcgcaccgat cattcctccg gcaaaccatc
     9721 ctaataaaat ccctagcgga ccacctaata caccaaccaa cattccgatt aatccacccg
     9781 tagaagattt gttattaccg gtaaagtcga taaaatcttc tattttgaat tgatgtgtcc
     9841 cattaggatc atggctgaca actgccatct gttctccttt aatcgaacga acagcatgta
     9901 actttttgat ttctgaaaat gcctgatatg cctggctttc tacttgaaaa tttaaaatga
     9961 ttactcgttt gctcattaaa aacgcccctt tcataccttc ttgttattta ttttaccttt
    10021 aattgaaaaa taagaaaaga aaagcactct ttttttccaa taaaataggg ctgtgacaaa
    10081 agtcgtatca cagctccttt ttccgaataa acggcgttct tgaagctgat cacttctgtc
    10141 acaacctccc ttaaatctga atctatattt tttggtattt gtttttattt gcttgcctat
    10201 tcgacaaatt caaattcaaa ctcaccgata cgtacaaggt cgccatcttt cgcaccgcgt
    10261 gcccgaagag cttcatccac acccatacca cgtaattgac gagcaaaacg catcactgtt
    10321 tcatcatgat cgaaattcgt catttggaat aatttttcga tcttctcacc agataagatc
    10381 cacgttgcat ctgaatcacg atcgatttgg aactcaggac cttctggatt gaatccatag
    10441 tgtacagttt cttcttccag ttcttcttcg tacaatggga attctggcgt tacttctaac
    10501 aaatctgctg tagcattcaa taacgcgtcc aatccttgtc gagtcactcc agaaattgga
    10561 aatactggga tgtcatcagc aaattcatct tctttttcct tgttcagctg ttctttgaat
    10621 ttcactagat tttccggtgc atccggcata tccattttgt ttgccacgat gatttgcgga
    10681 cgctctagca agcgtaagtt ataagtagat aattctttgt tgatggcaag atagtcttca
    10741 taaggatctc gtccttccat cccactcata tcgatcacat gtaaaattac tcgtgttctt
    10801 tcgatatgac gaaggaattg tgtacccagc ccaacacctt gcgaagctcc ttcaatcaag
    10861 cctggcaagt ctgctgcggc aaaacttctg ccatcacttg tagtaaccat tcctagatta
    10921 ggtacgagcg tcgtgaaatg ataagcgccg atttttggac gagcagatga aataacggat
    10981 agtaaagtcg atttcccgac agatggaaaa cctacaagtc caacgtctgc caatactttc
    11041 aattctagtt cgatttttcg ctcttgtcct ggttcaccgt tttcagcaat ttccggtgca
    11101 ggattccgag gagaggcaaa acggatattc cctcgtccac cgcgtccacc ttttgccaca
    11161 actagtgttt gaccattttc aattaaatct cctaaaagag caccagtctc cgcatctcgg
    11221 acagtggttc cttgtggtac tttaacatag gtatcttctg aaccacgacc gtgcatccct
    11281 ttactcatcc cattttcacc tggctgcgct ttgaaatggc gattgaaccg aaaatccatc
    11341 aatgtacgta acccttcatc gacgatcaag atgacatcgc cgccacgacc accgtctccg
    11401 ccagccggtc cgccgtcagg aacgtatttt tcgcgacgaa atgcaaccat tccgtctccg
    11461 cccttgccag ctttcacatc gatcgttact tgatctaaaa acatggacat aattaagtcc
    11521 tcctattatt catttattta tttgactgcc tgaatttcca caataaacaa aagtcttact
    11581 tattttatcg gtgaaatctg attttgtcta tcatttcagc aaaaagattg tttaccttct
    11641 tcttttttta tcatttggta aggtttccaa ccttttttct gctaccaagt ataatttttt
    11701 gacaaaatat tctttgcgta gcagaataac agtgtattaa ttgtattaat tgaggatgcc
    11761 gtttcacagc acccctaata aaggaggaac ttttgatatg acagtaaaag taggtattaa
    11821 cggatttggg cgtattggcc gtctggcttt ccgccgcatc aaagaagtct cagatgatat
    11881 tgaagtagta gcaatcaacg acttaacaag tccaactatg ttagcacaat tattgcaatt
    11941 tgactctact catggtactt atcctggcac agttactgca acagaagact caatcgtagt
    12001 ggatggcgaa gcaactcgtg tttatgcaga acccgacgca agcaaaatcc cttgggcaaa
    12061 agaaaacggt gtagatatcg tactagaatg tactggtttt tatacatctg aagaaaaagc
    12121 aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt tctgccccag ctggtgctat
    12181 gaaaaccatc gtctataacg tcaatgatga tacattagat gaaaacgaca aaatcatctc
    12241 agcaggttca tgtacaacaa actgtttagc acctatggct tatttcttaa ataatgaatt
    12301 tgggatcgaa gttggtacaa tgacaacagt tcatgcctac acttccacac aaatgctctt
    12361 agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt tcagctgctg acaatactat
    12421 cccacattct actggtgctg cgaaagcaat cggcttagtt atccctgagc tacaaggaaa
    12481 attacaagga cacgcacaac gtgttccagt cgttgatggt tcattaactg aattggtttc
    12541 agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa gcaatcaaaa aacacacagt
    12601 tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt tctagcgatg tcattggaac
    12661 aactcaaggt tcgatctttg acccaacgca aacagaagta acaactgctg gtgatttcca
    12721 acttgttaaa actgttgcat ggtatgacaa tgagtatgga tttacttgcc aaatgattcg
    12781 cttactagaa aaattcgcta acttgtaatt tgatagatca atcgataaaa gaagcctttg
    12841 actggatcca gtcaaaggct tctttctttt catcccctac ttttaaactc gttcaatgaa
    12901 catattggct taaaaattgt tggactactt gctgatattt ttcaggatct gtcttgtatg
    12961 cttccgcatg ctcggctcct tttacaacat acttttcttt tggtcccttc gttgcacgat
    13021 agaccttatc taacatttca aatggtacaa atgtatcttc gtctccatgt ataaagagca
    13081 ttgggcgctg gtttttcttc aattgttcca ctgcatttgc ttcaccgaaa aagtagccag
    13141 cacggacttt tgtcactaga ctcgttactt gcataagcgg aaatgctgga agactaaaca
    13201 tatcgtctag ctgataagca agctcatcat gaacggaaga ataaccgcaa tcttcaatga
    13261 ttgctttcac attatctggt agtttttctc cgctagtcat cataactgtt gctgctccca
    13321 tgctgacacc gtatagaacg atttcttgcg attttccatt gttttctagc actttgttta
    13381 tccactgtac atagtcttta cgctctggcc atccaaatcc aatgtaatcc ccttcgcttt
    13441 ttccatgtcc tcttgcatca ggaacaagga cattgtaacc catatcatga aacatctttg
    13501 cgtaattggt catcgtttca gcgtttccca tatagccatg agccacaaga gctgtttttt
    13561 cacttttcgt ttcagcagga agataaatcg ctgataattt cagaccgtct tttgaagtga
    13621 tggtccaatc gcttcggttt tcttttgaac taaaccattt atgtgcatcc gtttctttgg
    13681 atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc tttctcagaa ggcacaacag
    13741 cataattata aaaataattc cctgcaaaca ctaacgcaac aatcagagct aaagcgacgc
    13801 ctaaaaggat tttcatccaa agtttcataa atcatccatc tcatttctct tttttcatat
    13861 aaccttgaat tagtataatc aaagaatctc tactatgcaa tgttcatttt tataacccgc
    13921 gaattgtaaa attaagttag aaaaataaaa aggcatttat ggtacactca aattgtattt
    13981 ccgacgaaag aaaacaaagg agtgtaacca taaatgacct acaaacatct taccatagac
    14041 gaactgacaa tgatagaatc atattatctt caacataata agccggttga aatcgctaac
    14101 cgaatgggac gtgctataca aactatttat aatgtagtca ataagttcaa gcaaggcaag
    14161 actgctcttg attattggca ccagtataaa gaaaataaga aaaaatgtgg tagaaaagtc
    14221 attcaattac ctgctcatga agtagattac attaaagaga aagtcactct tggttggacg
    14281 cctgacgtca ttatcgggcg aaaagaaagg tctgtttcat gcggtatgag aacactttat
    14341 cgtttatttt ctaaaggaat atttgatatt gacacactac cgatgaaagg taaaagaaaa
    14401 cccaatggcc atcaggaaaa acggggaaaa caacagtatc agcgctcaat ccatgatcga
    14461 cctgataatt atcctgattt caattctgag tttggtcacc ttgaaggtga tacgatcgtt
    14521 ggcattcatc ataaaagtgc cgtcattact ttagttgaaa gattatctaa agtcattatc
    14581 acgattaaac ccaacggccg taaggcatta gatattgaaa ctgcccttaa tcaatggttt
    14641 tctcgcttcc ctaaaaactt ctttaaatct attacgtttg actgtggaaa agaattttct
    14701 aactggaaag ccattagtaa ccaacatgat attgatatat attttgcgga ccctggaaca
    14761 ccttctcaac gcccattaaa cgagaattct aacgggattc tgcgtcgtaa tggactgccg
    14821 aaatcaatgg attttagaaa agtgaatcag acatttattt ccagtgtcag caatcaacgt
    14881 aatcatattc caagaaaatc attgaattac agaacaccaa tcgaaatatt tttgagctat
    14941 gtacaagaag cattttattc tagcttaatt tgacaaatca taacctataa aaaaacaaaa
    15001 taaaaaagca gaaataactg gctatttcta gcggattatt tctgcaataa ctaattatct
    15061 attttttatt ttcaattaat ttaaagcttg accatggttt caaaaagtca aggaggaata
    15121 attcgtcatc ggagattctt cctacaacat tgacacgtcc gtcgtttttc atatccttca
    15181 aggcgatttg tgtttctcct ttgtattgac cgtacccttc attgtcaatc agtacatccc
    15241 catgatgcat atctcgcgta ttatgaggcg ggaaatcttt atctttgtag tatacacgag
    15301 tcatcgttga acgaaggata tattctgagc gatcccctcg ataactatga aggttatcaa
    15361 acaagcagat tcgttcgttt tctgtaatac cttcttccgt atctactttc aatgttggat
    15421 aatccgcgtt aaatgcttct gccatttctt tcaattcttc ttcacttgcg tacgcatttc
    15481 caatagaaat atcatcaatc aatcctgtaa ggattaaatg cttcacttgt gtcgcaattt
    15541 caagatctcg gtggtcttcc agcgtgcaca agccatcttg cgtaggccaa gggccaaact
    15601 cagcagattg cgaattgacg aatgccatcg tattcaaatt atattttctg aatttttctg
    15661 aacaaaaaac aaagtgttcg tatcctaaac cagagtatcg atgaggataa aaattatgag
    15721 aacctaataa attatccgtg tttggcgaat aactcatgat gttgtcaacg taattcgtgc
    15781 ccgaactcat attgatctcg attttgatac caaaaggatt gcgcgtcatt tttgcttctt
    15841 ctgctcctgt gaatccgata tctaagcgga ccccgtaggc acccatctta tggaaaaaag
    15901 ataagtcatc gtaagaaatc tccaactgtt caaataacgc tggattgata tcaaccatta
    15961 cttccatgcc taggctattc gcgtaatcaa ctacttcttt aaattctgaa agaacttttt
    16021 ctttatcatc attgatctgc agcaaactgg taaaaacccg tttataccca tacttatgag
    16081 ctaaatccaa gtacgctttg tccttttcaa atgttgaccg ttctggataa atcgagattc
    16141 ctaattttcc catattacca tttcctcctg catcttttcg tttttgtatc cacttacatt
    16201 atagcaaaca caaagtaaat ggtctacacc ttttcttttt cttttaaaat aaatgataaa
    16261 ttccaataat ttaagtaggt attttccctc tattttgtta tcattaaaga taacaaaata
    16321 gtaaattgga aaggaacagt tatgaagaaa aataaagtta ttttaagtat tagtttagca
    16381 gcagcattgc tgtttgctgc tggttgttct gcaaacaaca cgacttcttc tagttcgtct
    16441 acagaaagtt caaaaagtac atctactgcg gaaagcacga ctgccagtag cgtcgatttg
    16501 aacgcattgg aattaccaca attgacgaaa gatgtcgccg aaaatgaaga tctggtcgaa
    16561 atggtgacga ataaaggcac aatcaaaatc aaattatttc ccgaacacgc accaaaagcg
    16621 gttgaaaact ttatgacaca tgcaaaagac ggttactaca acggactgac tttccatcgc
    16681 gtcatcaaag atttcatgat ccaaggtggc gatcctaaag gcgatggcac tggaggggaa
    16741 agcatttggg gagaaggatt tgaaacagag atcaacaacc acctttataa tataagaggt
    16801 gcgttatcta tggctcgctc acaagatcct aacagtaacg gcagccagtt ctttatcgtt
    16861 caaaatacag atgatatgca tgatggtctt ttaaaagacg actatcctca agcaatcatc
    16921 gacgcttata aaaacggcgg ttatccttca ctagatggcc attatactgt cttcggccaa
    16981 gtgattgaag gaatggatgt cgtggatgct attgcaaata tcgaaacaga tagttcagac
    17041 aaaccaaaag aagaagtaaa aatcgataaa atcaatatac tgcaagaagc aaaacaaaaa
    17101 taaaaaaata acgtctgaat cctgcataga tgacaacgta tgctaggatt caggcgtctt
    17161 ctttttacat aaaaagcaag taagcttcct tttgttttgc tatatcaggt tttatagtca
    17221 agcattttgg ttgacgatga acgtgaactc gcactcacag acttttttat catctacata
    17281 tgctgcagat aacgctgtac cgactgatcc cttcattttg gtgatttcaa aatgacattt
    17341 cagcacatct cctggaacaa ctttttgacg gaatttggcc ttattgatcc caccgatata
    17401 agctcttttt cctagaaact cttctgattt taggataagt atcgaacctg cttgtgccag
    17461 tgtttctaga atcaacgcac ccggcatcac tggattgtca ggaaagtgac cttggaaaaa
    17521 atcttcattg attgttacat tttttgtcgc aacgatcttt tttcctggtt ctaaagtatc
    17581 tacataatca atataacaaa taggataacg attaggaatc aattccatta cttcagtagc
    17641 agataatacg taagacataa ttaaactcct tctttcaggc aattgccatt ttcattactt
    17701 tgactatcaa agtatcatat tcctaaat
//
LOCUS       DADVXU010000057        17484 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.059, whole genome shotgun sequence.
ACCESSION   DADVXU010000057 DADVXU010000000
VERSION     DADVXU010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.059"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(170..715)
                     /locus_tag="KB953_001783"
     CDS             complement(170..715)
                     /locus_tag="KB953_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBB6866757.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(727..1419)
                     /locus_tag="KB953_001784"
     CDS             complement(727..1419)
                     /locus_tag="KB953_001784"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBB6866758.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1438..1722)
                     /locus_tag="KB953_001785"
     CDS             complement(1438..1722)
                     /locus_tag="KB953_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866759.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1735..2298)
                     /locus_tag="KB953_001786"
     CDS             complement(1735..2298)
                     /locus_tag="KB953_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBB6866760.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2526..3188
                     /locus_tag="KB953_001787"
     CDS             2526..3188
                     /locus_tag="KB953_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HBB6866761.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3312..4508
                     /locus_tag="KB953_001788"
     CDS             3312..4508
                     /locus_tag="KB953_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBB6866762.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4609..5985)
                     /locus_tag="KB953_001789"
     CDS             complement(4609..5985)
                     /locus_tag="KB953_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBB6866763.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(6022..6889)
                     /locus_tag="KB953_001790"
                     /pseudo
     CDS             complement(6022..6889)
                     /locus_tag="KB953_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
     gene            complement(6978..8003)
                     /locus_tag="KB953_001791"
     CDS             complement(6978..8003)
                     /locus_tag="KB953_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBB6866764.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(8021..8695)
                     /locus_tag="KB953_001792"
     CDS             complement(8021..8695)
                     /locus_tag="KB953_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBB6866765.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8879..9772)
                     /locus_tag="KB953_001793"
     CDS             complement(8879..9772)
                     /locus_tag="KB953_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBB6866766.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9846..11543)
                     /locus_tag="KB953_001794"
     CDS             complement(9846..11543)
                     /locus_tag="KB953_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBB6866767.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11536..12849)
                     /locus_tag="KB953_001795"
     CDS             complement(11536..12849)
                     /locus_tag="KB953_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBB6866768.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12849..16886)
                     /locus_tag="KB953_001796"
     CDS             complement(12849..16886)
                     /locus_tag="KB953_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBB6866769.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16899..17357)
                     /locus_tag="KB953_001797"
     CDS             complement(16899..17357)
                     /locus_tag="KB953_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBB6866770.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
BASE COUNT         4927 a         3907 c         3030 g         5620 t
ORIGIN      
        1 aaaaagaaaa aatataactt aagttgttga taataaagag atttattaac gtaaaaagaa
       61 taagtttttt taaaataacg tataaaaaag agcataaaac aaagtcgttt gttttatgct
      121 cttcttttat tattttgtgc tttttatttt atcaaatcac tttgtttttt tataaaattt
      181 ctgcgcctag tgtggactca aaatgtctca atgcccattc atgtccaacg ggatcgaaac
      241 ttgccacgga atctttgaaa atatggagtt tgtatcctag attatacgca tcgactgctg
      301 tatgcaacac acagatatct gtacatacgc cggtcaaata aatatctgtg atctggcgtt
      361 ctcgcaaacg gatgtctaga tctgttccac taaaagcgga ataatgccgt ttgtctatcc
      421 aataaacatt ttcctcttgc tcatagtctt ggtataaact ttttaaagag ccgtacaatt
      481 cccgtccacc cgttcctatc acattatgcg gaggaaataa gcggttctcc ggatgaaagg
      541 aatcttttgg atcatgcgca tcaatggcaa aaacgacaaa atccttttga tcaataaact
      601 tctttgtatg agcagtcagc tttttttcaa ttacttgtcc tgctgtccca gtagtcagct
      661 ttccatcctc tgcaacaaaa tcatatgtgt agtcaatcga gatcaatgcg ttcatcttag
      721 tcctccttat acgagatgtt tgacaaattc gatcaccatt tctgctgatc gtttgcctgc
      781 gtcttcgata aattcgtcaa agctttgtgt cgccgagtga tctgctgtgt cgctcattgc
      841 acgtacgatc aaaaatggga tattgaactg acgagctgtt tgtccaacag cggctccttc
      901 catttcgcaa gctaatgctt cagggaaatt cgttaagatt tctttgattt tatctggcga
      961 gtcgacaaat gtatcacctg taacgatcaa tccttctttt gcgttcaaat tcgttgcttt
     1021 tgctgcttta accatttcac tgcgcaaata tgtgcttgct tcataatata gaggcattcc
     1081 tggtaattgt cctgggttgt agccaaatcc tgttgcatct gcatcaaaat aagctacttt
     1141 gtctgatata acgatgtcac ctacttgtaa gccttcaccg attccgccag ctgaacctgt
     1201 attgatcacc atgttcacgc cgtattgttg gatcaacaga cttgtggtaa tagatgctaa
     1261 gacttttcct ataccggaac gtacgacgat cacttcgtgg ttacctaatg aacctgaaat
     1321 aaataaagcg cctgcgcgtt cccatgataa tggttcgctt aggttctctc tcaaaatttt
     1381 tacttcttct tccattgcgc cgataatacc gattttcatc tttttaattc cttctttcta
     1441 tataaatgcg attgctaaaa aaaccacaca taacattagg ataacaataa taagagattt
     1501 catcaaaaaa gagttccatt tggtcgtctt ttcccgttcg ctgatccttg ttttcgtgat
     1561 tggtttattc tttttgcttt ctttacgata aaagctggca atcttttttt cttcttgctg
     1621 ataagctgtt tctgcttttc gctgtttttt taatgactcg cttgcttgtg cttgttgtcg
     1681 cttgcgaagt tcgcttcgcg taataagcgg tcctttggcc ataagccgat ccctctactt
     1741 tcctttggtc aatagttccc agtattgaat cgcataaatc gtctttgcat cacaaatcaa
     1801 ctgatctttc atcgcttgtt gcgcctcttc aagagttaga tgatacaact ccagtacttc
     1861 gtcttcatct tgtgctaaag gattctcgac cttctctacc ccttgtgcat gataaatatg
     1921 aaggacttca tttgcaaaac ctggtgaaag atacatcgaa gtaagatggc tcagcgactt
     1981 cgcacgatag ccagtttctt cttctaattc tctagctgct gtcatttcgg gattttgtcc
     2041 ttctcctgga tcgatttttc cagctggaat ctccaaaatc actttttcta aaggttttcg
     2101 aaattgtttg acaagaagca gacgatcttg ttcgtcaaaa gcaatgatcc cgacaccgcc
     2161 aggatgaaat accagttctc tcttcgaagt tcctccatct ggcaaacgaa cttcgtctac
     2221 agcaacatca ataatttttc cgctatagat ttctttgcga ctgattgttt tttcttcaaa
     2281 ttgctcattg ttcagcatat cttctcctgc tttctttgta atcataactg cttttttttc
     2341 atttttcaat ttttctcaaa aactccttat tagtttacca tagataaacc ttagattttc
     2401 tctaaatata gaaaatcctt ctttagtccg atactctttc tagtagtccg tatgctaaaa
     2461 taagtatgta atcgaataac gaaaagaatt caatactcaa cgataaatga catggagggt
     2521 ttattatgat aaaacgaaat tggccgtggc ttctagcatt ggtcttgcta tttctctttg
     2581 tccgaaacaa tagtttttct tttgttctag gacttttatt gataggtgta gttttagttt
     2641 tgtggggact ctttggttcg cgcaggaaaa agggaaaaca agaagaaatt cctgcactga
     2701 ctgacgaact tgaaactcac tatgaaaaaa gcggtatgac agcgaatgaa atttcatttt
     2761 ttcgacaaac aatgaatcaa acaaaaaatg agatcaatca attgcaaatg aatatgcagc
     2821 agacagccaa acttaaagca atcgatctcc gccatgatcc agtcaaagca gcgaaagctt
     2881 tattcaaaga attggtgaaa gaaccaaacc gactgcatga agcaagtcag tttctttata
     2941 cgcatctgcc gaatctagtt gatttgacaa ataagtataa tgagatcaat gaccatgaga
     3001 tcaaaaacaa acagacctat gaaaaactag aagaaagcgc gcagatcatc gaccagctgt
     3061 cagctttaat cgcacaagat tatcaaaaat tcgttgctga ggatctagat gatatcgacg
     3121 tagagatatc tgttgcgaaa cagagcttaa agcgtgataa taaatatgaa ccagaacaaa
     3181 aggattaact cttttgttca taaaagaaga ctcggaacat tcagatgatg tctggctgtt
     3241 tcgagtactt tttctaaagt agagacattc atgtaagaat cgagaacaga ttcaacaagg
     3301 aggaattttt tatggatcat aaaccgactg aaacaaaaga tgttacacca gttaacgata
     3361 cacttgaaga tttattgaac aacccgtttt caactccaac agatacgctg accacttcac
     3421 aacaagcgga gattgatcag ctccaagagc aacagacagc tgctcgttta attgacaaac
     3481 taccggcgga acgccaagaa caagctaaac agctagcagc aaaaatcgat gctaatgatg
     3541 cacagtctgt catcagctac ggatcggctg cccaagcaaa attaagtgaa ttctcacaat
     3601 cgatgttgaa tcatgtgcaa gcacaagata ttggtcctgt aggagattcc ttaactgaac
     3661 tgatgtatcg tctccaagaa gccaatcctg acgaactgcg tgccggagaa gggaacatct
     3721 tccagcgtgt cttcgggaag gtcaaacaat cgatctatga agtgacagca aaatatcaaa
     3781 agattggtgc acaaattgac aagatttcag tgaaattaga taaagaaaaa gacggtctgc
     3841 taaaagataa tctgatgtta gaacagcttt accagaaaaa caaagactac tttgatgcac
     3901 tgaatattta tatcgctgct ggagaattga aaatggaaga acttcaaaca acgatcattc
     3961 cagaagcgat gaaaagagca gaagaaactg gcgatcagat ggatgttcag attgccaatg
     4021 attacacgca attcctagat cgtctggata aacgtacgca tgatcttcgc ttagctagac
     4081 agatcacgat ccagcaagca ccacaaatcc gtttgatcca aaacacaaat caggccttgg
     4141 ctgaaaagat ccaagcatct atcgctacag ccattccgct gtggaaaaac caagtcgtga
     4201 tcgcattgac cttgcttcgt caaaaagatg cagtaactgc tcaacgccaa gtctctgaaa
     4261 caacaaacga cttattgaag aaaaattcag aaatgctgaa aatctcagcc atcgaaacag
     4321 cgaaagaaaa tgaacgtgga atcgtggata tcgagaccct tcaaaccact caaaatgatt
     4381 tgatcgaaac aatccaagag acattgcgta tccaaaaaga aggaaaagaa aaacgtcgtc
     4441 atgcagaagt agagcttgga cacatggaag aagatctgaa acaaaaactg ttggatttaa
     4501 cccaatagat cacctttcgc ttgttactaa aacaaacgcc caacaacagt ttattacaac
     4561 tgttgatggg cgtttttctt atctttcttc taacttctcc attggagtct atttctcatt
     4621 catcttgctg tgtccatagc catagccaaa ataaatgatc agtccaataa ccattacgat
     4681 accgaacacg atccatgtca cagtttgcag acgaagcatc aatacaatac aagtcaaaat
     4741 ggagacgatt ggcaatagcg gaacgaatgg cactttgaat tctccagctt ttggtttgcc
     4801 aaacattttt ctcaacttca gtaagcctag actaacaagc atcaaatagg cgagcgccat
     4861 gatattcgtt agttccgcta atacattcaa tgggaaaaat ccggcaaaga atacagctgc
     4921 cacaccagcg gcataagtcg cttttttcgg tgtacgatgc ttttgatcga tttctgtcat
     4981 aaacttagga agtaagccat ccttgctgac agcaaacagt aaacgagcaa gtgaatacat
     5041 catcgagatc gttaccgtca gtagcgttaa aattgcccct acagcgatca ccgcgcccac
     5101 tgttccatgt cctacatacc tcattgcaaa agccactggg tcattgactc ctagttcagt
     5161 atacggaacg attcctgtca agatcagcgt cacgatgatg taaaggacag tagcaatcag
     5221 tatcgaacca atgattcctc ttggcacatc tttttgcggg tttttgactt cctcggctgc
     5281 cattgcaacg gcatcaaatc ccaagaaagc aaaaaacaca agagctgcac catctagcac
     5341 accggaaaaa ccaaaaggca tgaatggctg ccagttatca ggtttaacga aaaatattcc
     5401 tacaatgata aacaacgcga tgataccaaa tttgacatat accatggcat tattcaaacg
     5461 taatgctttc ttggcttcaa gcgacacgat gtaagtgacg aatatcacta cgagcaccgc
     5521 aatcaagtca atgtatgtac cattttccgg attgtacccg ccagttaatg cttgtggaag
     5581 ttcggcccct aaacttttca aaaaaccttg catatagcca gaccatccgg aagcaacaga
     5641 ggaaaccgct agtaaaaatt ccccgatcaa caaccaccct gtcagccagg cagcaaattc
     5701 accaaataca acatacagat aggcatatgg cccaccaatc actggcactc gcgaagcaaa
     5761 ttctgcgaaa cttagtccag acaaaatgac aacgattgca gctaagacaa atgaaagtga
     5821 cagtgctgga ccagcatttt gattggccgc aacccctgtc accacaaata ttcccgtacc
     5881 gatgatcgca ccaattccaa gcatgatcaa atcagacgtt tttagttctt ttttcatctc
     5941 actcgtatga ttgagattga tctctttctt acgaaacaaa tccattgaag ttcctcccca
     6001 ttttaggttg gaaaacagcg attaaactgt tttcatatac cctaaaaggg ataccaccta
     6061 aaatgactaa aaggcaacca acaataacat aaatgagctc ttttttcgtt tttctttcgc
     6121 caaggatgaa aatcccacct aaagtagaga taatgatccc catttgtgaa agtgagaagc
     6181 caacagctaa accaagtgat ttgactgtta gaagcatgca gatgtttcct aagccccata
     6241 ataatccaca gatcatgttt ttccacacaa actgatcgac tttaacttta cggaaagcaa
     6301 agaagcttgc gcctaagatc attccgatac tttgaggtaa gataattgac atcgcatcca
     6361 aattcgccca gttgacgatg atggtatata caccataacc aatcgtagag aagatcaatg
     6421 ctctaaaacc cttaccaaaa tcaagcattt tattatctgt atctattttt ccttcgtcat
     6481 cttgtcgggc agtcaaatac gcaccgctta caagaagagc taaagcaata ataccgtata
     6541 cataatcttt cgtttgtgtc cattcatgga agaaaacggc tcccgcaatc gtattcaaaa
     6601 ttaattgaaa accagtagac aatggtagcc ctacagaaac acccatgtac ttcattccat
     6661 ggaactgtcc attttgtcca acactccaag ctaagccgga taaaaatccg atcaagaaaa
     6721 tccaaggggt gattacagga ctaacaacga aaaagacaac taaagaaaac agaaatgcac
     6781 cgatcgtcat tcctagtgtt tgttgattgg catcgccacc aattttacca cttactaaac
     6841 caatactacc ccatgccaac atagggacta atgctattaa aatttccata accaatctcc
     6901 ttttatttca catgataaga aacttatcag cgaaaacgtt aacaacacaa gcactttttt
     6961 tcgtttctaa cgattttcta acgtttttca ttataaaaat ttaagattgg gaaaaataaa
     7021 gtcattgttg tcccttttcc ttcagttgat tcagccgtca aatggatacc taatttttcg
     7081 gccagatttt ttgctaaata aagtcctaat cctgttgaat gtgttttgct aagtcgacca
     7141 tttctcccag taaatccctt atcaaagatc cgtttcaagt cttccgctgg gataccgatc
     7201 cctgaatctt taactgaaag gctgactcct tgcgcttcct tttctatgct cacaatgatg
     7261 tttccgtgat ccggcgtata tttgatggca ttactaagca attggttgaa gataaaagcg
     7321 atccatttag catcggtcaa aacagtatga tcctcaccaa cgatctcgta gcgaatattt
     7381 ttttggataa aataatttgc ctgactgcgt atcacaggct gaatgattcc ttttagtcca
     7441 tattcttgga tgaggtagtc ccgcgagaaa ctgtcgagtc ttgcataata caaaacttgt
     7501 tctacgtatt catcgatttt tgataattca ttttctagta atgtatactt ttgatcgtca
     7561 atatcgaact cgatcgaacg aactagcaat ttagaagcgg aaagtggcac tttgatttca
     7621 tggacccatg agtcaatata atctttttgg tcttgttgat tactgattgc tgattgcatt
     7681 acttcttggt gttcacggat cagctgattg atatattctt ctaccagttt ttcttcttct
     7741 gtcctcccac cactcaaata attttgcaaa ggggaagttt tttgtatgtc ggctaatttt
     7801 ccccaccatc ttctcttgct caagtaacca cccagcagat aaaaaatcag aaagacgccc
     7861 tcaatcaaag ctaaataagc aattgtgctc aaatcaactt tgatgtttgg cgccagccac
     7921 atcacaaaga tcgtcaatgc aataaaaaac agccatccca agatcaatgt ccactgatct
     7981 ttcaaatatt taaaaaaatt catcttaacc gctcatcctt tcagggaacg atatatccct
     8041 gtcctacttt tgtttcgata tatccttgga tacctgcctg ttcgattttt cggcgtaacc
     8101 gattgatgtt gactgtcaat gtattgtcat ctacaaatcg ttcgtcatcc cacagtgctc
     8161 gcagcaattt ttcccgactt aagatctttc cattgtgctt catcaaaata aataatagac
     8221 gatattcatt tttgctcaaa tcaatgatct ctccgttgat ttccatactt ccgttgtcaa
     8281 cattcaacgt aatgccattg tggctcatca tttcactaga taattctgtg tagttgtaag
     8341 aacggcgaag taatgcattg atttttgcaa tcagtacatc gatttggaaa tgttttgtca
     8401 caaaatcatc tgcccccatg ttcatagcca tgatcatatc catattcgta ttcctgctgg
     8461 agataaaaat gatcggtact ttcgatactt cacgtatttt ttgacaccaa taatagccat
     8521 ccaaaacggg aagattaata tctagtaata caagctgtgg ttcttctctt tcaaagtctg
     8581 agaacacttg attgaaatcg gtcgttccaa aggtttcaaa ttgccatttc tcaagttcct
     8641 ccatgatcaa ttgtcgaatg atctcttcgt cttcgactac catgattttt gccataaaaa
     8701 atccctccat ctttcttctt gctactacaa tatcaaagaa gcccttgtga aacaagttgg
     8761 cttacctgga aaatgtcatt ttagcaaacg ttaaaaaaaa ctggaaatat gcgatttaat
     8821 cgcgtttgtc tcttccaagc agcttaaatt tattatgtta ttcttaataa ttggtctatt
     8881 atctgttatt tttctttcgt tgtttttgtc ttttaaattt tcgataacac caataaccga
     8941 aaagcgttag cactagcaat atacctagaa ggagtataag taatcgataa agatggtatt
     9001 tttttgtctg ttctatcttt gaagagactt cttctgtaaa gggaactcgt cttcctgtca
     9061 ctagtaatcg gtgagtgttc acggtgtaag gtgtacaagt gataagtgtg actagatcct
     9121 gcttttctct aattgctaat gtatttgttt ttgttggaag gacaacaatt ttctcaacga
     9181 cttcataagc gagcatccga ccatacactt caagatagaa tcggtcatct tgctccattt
     9241 cttcaagatc cgtaaagagt tttttctcag caataccgct atgggcggta atcacggaat
     9301 gtgtactctc tcctccagtt ggaaaagagg tgccttgcag gactgtcgct cctctatcta
     9361 acaaaagatc attcgtttca tcaaataaag gcaagctaac attgattttc ggaatataga
     9421 tggctccgat catatgttgt tcgtaatatt cttttcctgg attttggaca tttctaaccg
     9481 cttgctcaaa aggatctttg acttgtccca ttcctgggat tactgatgta tgagcaagcg
     9541 tctgattttt cttttcatgc actgctaatt gttgctcatg tttttcttct ttatcgcgtt
     9601 caagctgctt ttgataattc tcgatccttc tttgatccaa ataattgctg agagcatctg
     9661 ctaaaaatgg atatgtaaaa actgcagctc cggcaagaaa catcactgcc ataaataatt
     9721 gtaataaaag actgatttgg gtttgaggtc tattacctat tttctttttc attttcttca
     9781 ccttcttatt taaaaaaact gcagcaacct ttataaaacc gattgctgca gcttattttt
     9841 gttgcttata cttcagactt cattttagat ttacggtacc agctgtacgc tccaatcatc
     9901 aaacttattc cgattagtaa gaatgccaag agcccgttcc caccagtagc aggaagtgac
     9961 ccgcgtttca tattcggtac tctttctggc cctggatttt ctactcctac gatctgaatt
    10021 tcttcattat agcttccata agagatgata aattcagtaa aggctgcctc acctgttgga
    10081 agtacataac cttctggcgc catggtctct ttcatttgat aggttccata atctaaccct
    10141 tgaacctcaa agtatccatt tccatctgcg acaaattctg ttgcttgtcc gcttgttgcc
    10201 cactcaattc tatctgctcc tgattcataa acagctaaag gcgactcccc tttataaaat
    10261 attgcgtatt cagaacggtc ttcattccac aattcgaatc ttgcatcttt aagcaattca
    10321 ctgcttgatg catcatattt ttcaaacttt cttccacctg ttgtgacttt ctctggcggc
    10381 tctactcctt cttcataatc aaattcatca tttcgtccga tttcaataac cgctgtgttg
    10441 ttatggaaat catccgcttc tagttctgtg gaaaccttca aattatacgt gatcgttaaa
    10501 tctcctccac gtgcatctgc taatctacct aaagtatcta ctttggctac tccattttct
    10561 tctgtcaaaa tcgtcacgac aaacccgttg cttactactt ctactatata atcttgtcct
    10621 tttgttaaaa tcgtctcgcc ggctcgcact tctatttcct gatcataata agttagacca
    10681 actgtaggcg tatctgtgat tttgtaattt ttgataacag cgcttccgtt ttcaaggaca
    10741 gaatcgatga aataaggaat aggtgctgtg atcgtgtaag aagccgtttt tcctctttct
    10801 agatcaatat aagaaatcgt ttcttgttct ccagcttcat taataatcgt tacttgtcta
    10861 aggtcgcttt cctctacatt catctgttta tctgcttctc tgatttcgtt tttcggatac
    10921 aaatgcacat ctgtattgta tgtgtctttc catgtagaac catcccacac gccatccacc
    10981 atatcaggca tcatcactgg catcataaca accattggag acgcgattct ggaaatatga
    11041 gctggcgaat aagtttctaa gaaaagatag actttatctc tagaagaagg tgcttcttcc
    11101 gtcacttgga cggtagagaa taccgcttct ccagcttcat tcgttgtttg tttgtcaata
    11161 cttgtcagtc gatattttaa aaaccaactt tctgtgtgcc gtttttgaat ccattcaatc
    11221 gcttcggcag aattaagacc gtcttctcgt tccggtgtta aaggattatc tcgaatcaaa
    11281 tcgtaatata cttccgttac gtcaaacaaa tcaaaatcga ctcctggaat attttcttca
    11341 ccaaaatccg tagaaaccaa tccgctattc ggtcgttctt ccggaacaga tgagaaacct
    11401 tttttgtgta atgtaatcgt cacttcactt ggtttttgtg cgacttcttc ggctttgact
    11461 agttttccgt tcccgaaaat ccctattccc gcagtaacga gtaacagtac actcattccc
    11521 gtgacaatca tttttttatt catttttttc tccccgttct ttttttatta ataaaacagc
    11581 acctaacaat aaaagaattc ctagtaaaga aatcaacgtt cttgcttcat tcgtttgcgg
    11641 gaatacggac cattttcctg attgacctgg attgcttgga ccactcggct ctttcggttt
    11701 gtccgttgtt tcttttacgg tctcgttata aacacgtggt gttgcggatt cataggcaga
    11761 gactggaact ttcccggatt ctaattcagc catctcctgt ccgttaacta ctacatattg
    11821 tggttggcct tttgcatcca cccaaaattc agggatgatg acctcgattc tcttacttgc
    11881 ttcatccatc tcgtaacctt ccacaccttt taattcttca aaataatagg tgccagaagg
    11941 caagaaacgt tgtcctgtcg taacaaggcc ctctttatcc gaaatgaatt tgcttacatt
    12001 tttgtcattc aaaggatcat tgtctgctgg tttgatccat tgatttttca agtcattagc
    12061 aggagccata tctaaataat atttcccccc ttctatcatc tgatatagag caaaaacagc
    12121 accttctaat ggttttcctt tttcagttgt tcctctttga cgtccatact tgaagaagta
    12181 tggatctctt agataaccga catttttagg atagatatga atctcttgca gttcctcggt
    12241 tgattctgtc gggtgataaa ttggcaaaat agcagcaatt gggatagctg tctgatcgat
    12301 atcaacatcc aatccaactt ctggatcaat cgattcttcg aaaatcaaat aaactgcatc
    12361 ccgatcttgg tttttcttag gtgctttgat acgtgcaatc ccgtctccta atccgccaac
    12421 cgtatctttc ccagttgtta ccgaagtccc tatttggaca agcctttctt ctgtgattag
    12481 ctgacggata ttgtttctgt tcgtttcagc gatttgctgg acgaattctt taggagtcat
    12541 gttttcagca gctttttttt catgataata atcggtcatg tcgtaaatgg caaatgtcgc
    12601 tccatttagt ggcaccgtct gtgaaatgat atctgctgtt tcccctgctt cctcagagat
    12661 agccaagcca tcattttcat ggtattcaaa ttgatcgtta taatcgatat ctcgaatcat
    12721 ccgtttatgc aacacgaatt ccacgacatc ttcccctgat tcttctgccg acatcgcatg
    12781 tggaagaagg actgctgcaa gcggtagtaa aacaagtaat tgaagcatca aacacaacag
    12841 ctttttcatt accttgctcc ctccttttta gattgtctga caaacaaacc ataggctgct
    12901 cctgcaagcg ccatgaagaa tgcagcaagc aggatataga ataatcgtcc ggatcctcct
    12961 gtagcaggca acggattttc aggatcgttc ccgacactaa gctgtaatct gattcgattt
    13021 gtctccaaaa gttcatattc taactcttgc tgtagatcag gcgtctctgc ttcttcgttt
    13081 ggataatacg aaatctcaac ttttagtgta cccgcttctt ttcctggaac tacccgatca
    13141 ttttctatgt caggttcttc tcgataacta atttctaaaa taaagtaacc tggaagtttt
    13201 ctatagctgt caggtgactt cacttccttg atccgataga ccccttccaa caagtctgaa
    13261 gcattgaata gggaagaatc gtttggatct ggtgccaagc ctccctcgat gatttcatac
    13321 tctcctgcac tattttgttt ctcaattgaa aattctgcgc cttcaagacg tcggctattc
    13381 atactgtcaa ttttcaaaag ttccaaatcg attggcgcta gttcatcttc taaggttact
    13441 tgtagtacac catcgatgat ttcatagcct tccggcaagg gagcagtttc gtcttcgatg
    13501 accgtatctt caatgataaa cttaccttct tcattcagtg caaactggaa cacttgatca
    13561 gatagccgat acccatttgg tgcgacagat tccgagacct catattcacc aatagacaga
    13621 tgcagaggtt ggtcgtaatt taaatcgaca tacttttcta ctcctacggc acgagaatag
    13681 aagtatccga tcccaggcaa acgtttcgat tcttctggtt ctaacggatt accagtgtat
    13741 gctcgataca gcgggaagga agaatccccc actttcctga tatcgaaacc tgcaccatct
    13801 aatgtatggc cttcatggtc taattttcta actcggatct cccgataatc aaacgtattg
    13861 taagcagtga atgctaccca taaacgatcc tcttcatctg ttgtatacgt gagatattgc
    13921 caattttctt cacctgtttg cttgagttgg atagtcccta tagcattatc gtaaagtcgc
    13981 aagatccatg tgtcattttc atcaggttct ttgaaacctt ctgggttctg gctctcaatg
    14041 attttgtatt ctgcatagtt ggtagtttct tcactatcct tataaacaaa tgaatctggc
    14101 tctccgattg gatgaataac tttctcacca tccacgatta catatttccc gtcttcatct
    14161 actgaataga acgtgagtct ccctttctct tcctctactt cgttcacacc agaaagccct
    14221 ttcgctactg gatatccgat ttccagtccg ttaccatctg tttcataaag ttcaaactct
    14281 gcgccattga ttggcatatt actgccttca attagtttat tgatattcat gtggattggc
    14341 ttgaattcat ttcgcatttc cagtttgaca tacaactcat catactctgt gccttctatg
    14401 ttaggctctt gtacaacaga aagcttgtga tattctcctt cataatagga attcggatct
    14461 ttaccttccg gtccgccgac tctcgaacca gatgtatatt catctacaag ctcgtagtga
    14521 cctaacatct catcttcgtt ggctgcatat tttttgattt ggaattgata cgtctcatct
    14581 actaactgat aacgtggtgg tccagcgatc tctctgatcc aaaatatgtc gctgactgct
    14641 ccagtttcat ctcctaaaat cggcatcttc gtttcaaaga tcgctattcc gtccgtggta
    14701 gttttacttg tttcgattgg ttcggcttcc ccactctctg gattttggtc atataatgca
    14761 aattcgactc cagccaacgg ctcgccattt tgacccttct tgctgacttg gaaattacgg
    14821 cctttatgtt cgtaacgaat ggaagaagat gggaaataca tccgtccagt agtgatatca
    14881 ttctcataat cctccaggtt ctgaatatag gccatattgt tcactgtgca aaaatttccg
    14941 tcttgagctc ccgcatcaag ttttacttgg tacttgatcc tgaccatttc cccttcacct
    15001 aaagaaatcg gagaaaattt ataagcttgg ttttcccgtc cgacatacgt tgtcggagtc
    15061 gccactactt ctggtggggc ttgtccatga cgttcttcat cccatatttc tggctcttgg
    15121 tacttcatgc cgttttctcc tggatctaga tggtaaaact gaatatctgc cgatctgcca
    15181 gctccaaatg gtgagacatt aaaaccagtt tcatcataaa gtgtggcttg ctggatcgtt
    15241 ctcttgaagg tagaagagat atcctccaag gctgtaacaa tgtcattctt gttgttcgca
    15301 ttgtaatatc gcggtgttac gccatcttct tctaaatcag caatattttt caatacattt
    15361 cgtccaatag catctagtcg ttgtctcccg ctaacgtttc catcgattcc taaaccaatg
    15421 gagaagatac ttggcgggtt ctcttgatac ttgtttttca gccaatgatg ataagcaact
    15481 gtgttgatcg cagatgactt ttcttgcgaa gtaggactgc cattcgtatt gttattagaa
    15541 gaaccatcac caacccagtg agtcgcttta ttatctcctt cttttacctc tccataacga
    15601 tagtgtacat ttacttcatc ttctggacgg ttaaagtcag atgcataggt aactggatga
    15661 gtaaaccctg cattagaagt attcccagaa aactcctcaa aatttctgaa caaaacgtca
    15721 ttatcttcag caatcttatc ttccatcaca gtccagttgc tccaagctcc attagtgctc
    15781 gtacgataac ttgactgaat cggtgcataa ctgaaggtcg gtgtcccatc tcccacaacg
    15841 attaggattt tttctggatt tctattttct ccgccattgt ctttatacaa tgtttcatag
    15901 ccatttttaa gccctagtgt aagcggagtt cctccgattg gtgtacgatt taatgctgta
    15961 ttctcacttc ccgtcaaacg attgatatca tttgtcaatg gaaagatgtc cgaactgatt
    16021 tgctcatggt tattgatagc tgtactatta tggtaaaaat tcaccatacc gattcgaata
    16081 tccagactcg ggtcagagag cagatcttta ctcatttcgt ttactgcctc aatcaacgca
    16141 gcatttttag tttgactatt ggtcccagcg gttaattcac tcatactcgc tgacttatct
    16201 agaacaaaaa cgatatcgat tggactgatt tcctttttct cgcccccgat gacatctaga
    16261 tagacattgt aaagggttgt tgcatcttca ggatgtagat cagatgctgt gctgcttggc
    16321 agaacaggtt ctacccattt tttgatgtac gccccgttat aatccaagta actgttttca
    16381 aaattccgtt catttcccca aagctgaacg gtttgagctg gaacgtttct atcttcttcg
    16441 cctgcagttg atccattatt agtagcatag tcgtaatttc gccagttcgc tcccccgttt
    16501 gtataatcac tatcatcgat caagttctct ccgtcatatg gcgagccttt gtctccttga
    16561 tagcgttcat tgctttgtgt ccaatttcct aacacttttg tatagtcatt cgttgtaaac
    16621 ttcgggtagc gatgctgttc gttttcaact tctccgctag gatagatata ttcatagggg
    16681 tcttgcgtag ctgttggccc aagacgctcc aaaataccgt tgaataactg gcgttgaatg
    16741 cgctggagtg cttgaccgct gggtacgatt ggttcgtcca gcttttccat ctcattttct
    16801 tcgctacgtt gaatttcagc ggaatcttct gtatcagttt cattttcttg taattcttgg
    16861 ttatctattc tttcatcaac caccacttga agaggtagtt acccttcgtt cacatcgggg
    16921 atcttttctg tttcaaaaat caatgtgccg gtttctgctt cttttgaata aacttttccg
    16981 acataccatt cttgaccatc gatcgtctcg acttttaggt cttctccatc ttcatcatag
    17041 gaggatagac ctgttccacg aacgtttttt acggtaccaa tcccggcggc tgccgcatcg
    17101 attgctaaac gtattctacc ttcattggca ctttcctttt tttcaaaagc cagatgccat
    17161 tcaagggttt cttctttttc aatatacttc ccttcaattt ttgcatagtc ctcatctaag
    17221 aatacctcat cctcctttgt ttctgttgac tcagcagcta catccagggt cgtcatatga
    17281 tagactgact ggaaatatgg tccaaatagc aaactgacag ttactaactg caaaagcttc
    17341 aattttaacc ctttcacatc cttatactcc tttcaaaaat atttgtcttc tttcacaaca
    17401 atcaacaaaa caatgcccat ctaattataa tttaatttaa ttttaatttt aaatcaaatg
    17461 atatcctttt tagttaaaaa acac
//
LOCUS       DADVXU010000058        16762 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.061, whole genome shotgun sequence.
ACCESSION   DADVXU010000058 DADVXU010000000
VERSION     DADVXU010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.061"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(45..758)
                     /locus_tag="KB953_001798"
     CDS             complement(45..758)
                     /locus_tag="KB953_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBB6866771.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            933..2357
                     /locus_tag="KB953_001799"
     CDS             933..2357
                     /locus_tag="KB953_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBB6866772.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            2656..3363
                     /locus_tag="KB953_001800"
     CDS             2656..3363
                     /locus_tag="KB953_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBB6866773.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            3350..3673
                     /locus_tag="KB953_001801"
     CDS             3350..3673
                     /locus_tag="KB953_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBB6866774.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(3762..4799)
                     /locus_tag="KB953_001802"
     CDS             complement(3762..4799)
                     /locus_tag="KB953_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HBB6866775.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(5120..6103)
                     /locus_tag="KB953_001803"
     CDS             complement(5120..6103)
                     /locus_tag="KB953_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HBB6866776.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(6518..7288)
                     /locus_tag="KB953_001804"
     CDS             complement(6518..7288)
                     /locus_tag="KB953_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6866777.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(7281..8216)
                     /locus_tag="KB953_001805"
     CDS             complement(7281..8216)
                     /locus_tag="KB953_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6866778.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(8532..10181)
                     /locus_tag="KB953_001806"
     CDS             complement(8532..10181)
                     /locus_tag="KB953_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBB6866779.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(10298..12901)
                     /locus_tag="KB953_001807"
     CDS             complement(10298..12901)
                     /locus_tag="KB953_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBB6866780.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(12913..13962)
                     /locus_tag="KB953_001808"
     CDS             complement(12913..13962)
                     /locus_tag="KB953_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBB6866781.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(13988..14302)
                     /locus_tag="KB953_001809"
     CDS             complement(13988..14302)
                     /locus_tag="KB953_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBB6866782.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(14318..14764)
                     /locus_tag="KB953_001810"
     CDS             complement(14318..14764)
                     /locus_tag="KB953_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBB6866783.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(14765..16687)
                     /locus_tag="KB953_001811"
     CDS             complement(14765..16687)
                     /locus_tag="KB953_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBB6866784.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
BASE COUNT         4974 a         3408 c         2759 g         5621 t
ORIGIN      
        1 tttagtataa aaaaataagc tctgaaaaaa taattttccc gagcttattt ttcaacaaaa
       61 acagcttcaa aaacaaagtc ttgatataaa tgtctcgata ccgaatactc gaaaggttta
      121 ccagacgata gagcaacaac tttgtctatc tcaatcataa attcacctgg atctattttt
      181 aatatttttg cgtcaaattc attcgcagta tctcctttga tacgtatgtg cgaagtcttt
      241 atttccagac ccagttcatt tgtgatataa gcatatacag atttctttaa atgttttggt
      301 tccaagcctg gtatgactgc taatggcatg aatgtttgct caatcgagta tggttgacaa
      361 tcaataaaac ggcttcgtac aatattatat acatagtctt ttacactgat acttaattta
      421 tcagctactt ttttcgatgg aagttcaatt gagaaagtat ggatttctga atctacgtgt
      481 tcttcaccaa ccacgctaaa caaaccttcg tttggaccaa gaactcgtga gttatcaatt
      541 ggtgttctga cgacttcagt cccataccca cttctacttc gcaaaactcc ttcggaaaca
      601 agtaagtcta cgcctttctt tactgttaac ttgcttgtat gataaataac tgccaattca
      661 tctcctgtag gaagtttctc accaatttta tactctccat ttacaatttt tttctttata
      721 tctaagtaaa tttccatata cttcgcaatt tttttcaatg gtgaccctcc aatcaaaaac
      781 atatcttcat ctatacttta acataaataa accgataaaa tccacatcaa aattatacac
      841 acaataaaaa agatatatat atttaaacaa aactatttac tttaagttgt aagcggtgtt
      901 atcattaagt ggaaacagga ggtaaaaaaa ttatgacaac tatgaaaaag ctaacttttc
      961 cagatgattt ttggtggggt tccgcttggt cagctgagca agcagaagga aaaggagaaa
     1021 caggaaaagc agagactatc tgggatcgct ggtttaaaga acaaccttac cggttttatc
     1081 aacgaatcga ttcctctatt acaaccgatc acatccatca atataaagaa gatatagaac
     1141 taatgaaaaa aactgggcat aactcctttc gcgtttctat ttcttgggca cgaatgtttc
     1201 caaatggcgg taccggaaaa gtaaatccca aagccatttc tttctatcga aaattatttt
     1261 ccgaaatgaa taataacgga atcaaagtat ttgccaactt atatcatttt gacatgcctg
     1321 ctactcttca agataaagga ggatgggaat cacgtgaagt agttgatgct tatgttcatt
     1381 acgctgaaac ttgttttaaa gaatttggtg acttagtttg tcattggttt accttcaatg
     1441 aaccacttgg gcctatttta ggtacttatc ttgaagattt tcattatcca aacctcattg
     1501 actttaaacg cggagcgcaa gcagcattct tcactatact tgcacacgca aaagcaatac
     1561 aagtatttaa agaactagct cttcctagta aaataggcgt tattctaaat ttgagtccta
     1621 cttttcctcg tagtcaaaat cctgctgatg tgaaagcagc agaaatcgct gatttatttt
     1681 atacgcggag ctttttagat cctatggtta aagggaattt ttctaaaaag cttatcctgt
     1741 tattacaaga atataatcag cttcctgaag attatacgaa agaagatctt gtatgtatct
     1801 acgaaaatac cgctcaaatt cttggactaa actattacga gccacgtcgc gtcaaatctc
     1861 gttcttctat ggtggataca tctagtccat ttttaccaga gtggtttttc gaaccacata
     1921 tcatgcctgg acgacgaatg aacgaatacc ggggatggga aatttacgaa aaaggaattt
     1981 atgatttgtg catggatatc aagaaccatt atggaaacat cgaagcattt atctcggaga
     2041 acggaatggg cgttgcggat gaagaacgtt tcatggatga tacagggcaa ataattgatg
     2101 attatcggat tgagtatatc aaagatcatc ttgcttactt atggaaggca ataaatgaag
     2161 gatgtaacat taaggggtat catctttgga catttattga ttgctggtca tggatcaacg
     2221 cctataaaaa ccgatatggg ctggtttcac tcgaccttac tactcaaaaa agaacaatta
     2281 aaaaaagtgg cgaattttac aagcaattaa gtgatacaaa tggatttaga tacgatgtag
     2341 acttgttagt tgaataatat tctaaaattc cacttgaagt gaacacatta gaaaacaaga
     2401 ataatgagaa ataccaataa caaatacttt ttcttcatac ttcatttata cttgtcagta
     2461 taagagactg tgacaaatgt agtgatcctc agaagttagg tctggaaatc taacttgaga
     2521 agattttttt aaactacgtc cagtcttttt tatttgttta aaattgaagt tgacaagtct
     2581 tcattaaaaa tttagaatat tcacaagttt gacgacaatt atgaaaattt atgtcgtgct
     2641 aaaggagttt tgttattgga tgaaaagtta gatataaaaa ctgcgatcaa agacacgtta
     2701 cctaccgttt ttggttatat cggtattgga cttgcatttg gtatcgttgg gaaagctgcc
     2761 gggtttcatc cgctggtcgt cacgttgatg tccctgctga tctatgctgg ttctgcccaa
     2821 tttatcacgg tcagcatgct tgctagccat agtccactgc tctccatcgt tctctcgacc
     2881 tttctagtca attcccgaat gattctgatg agtatgacga ttgctcctta tttcaagaaa
     2941 aatcggctgc ttcaaaatct gctgattggc acattgctga cagatgaaag tttcgcactg
     3001 ggaatgaata aattgaacta tactggacaa aagttaaact ttcgttggat gaatacagct
     3061 aattggattt cttatttgac gtgggtgggt tcttctcttg ttggcgcact tttagggaat
     3121 tttattactg atccgaaaaa gtttggtttg gatttcgcca tcgtcgctat gtttatcggg
     3181 ttactttatc ttcaagtcat ttctgataga aacacctcta aacgattaca gttgatcctg
     3241 attggtctca ctttgattct ggtatatgtc ggactgattt ttatcccaag taacttagtg
     3301 attttagttg taacactgat tgggtgcgga ttgggggtgt ggatcaaaca tgccttcttt
     3361 tgagtttaca ttgctgacta ttttaggttg tactttagca acttggcttt ctaggatcct
     3421 accatttgtc ttactgaaaa aattcgattt gccgcaaccg ttgcttgagt atttaagttt
     3481 tgtcccgatc gtcattatgt ctgctttatg gttcagcagt ctatttaccc aaaatatcgg
     3541 acatctgccg cagatcaatc tcgaaaatgc tttggcttca gttcctacac ttttagctgc
     3601 tattctttca aaaagcttat tggtgatcgt cttggtagga attctttccc tttcgttgat
     3661 tcgcttatta taaataaaaa aacaagagct tatggcaaat tactgtcatg cgctcttgtt
     3721 tttatcaaag tcaaataaga aaaaaatcgg cgtattcttg cttatccgtt gaacggacca
     3781 ctaacaacga cttttcctaa tatatgtcct gtttctacgt ctttggttgc ttgtttcaag
     3841 ttcttcgcat tcatcccatc tgaatagacc ttgctcaatg tactgcggat aatcccttca
     3901 tttgctaaag cagcaatgtg cgctaaagct tctccttgcg tttcgatttc atagttatag
     3961 tctgttttag cgaacatata ctcccagtcg aagctgatag attggttttt ccaaacacct
     4021 atatctaacg gctctttgat ccctacgatc gttcccacat gtccaaatgg tcggacaagg
     4081 tgtttgattt gatcaaagta gcgggtaata tcgaataata cagcgacata ctctacttga
     4141 tcgattccta attcacgaaa acttttactt aagtcgtgat gataatcgat tggatagtct
     4201 acacctgttt ttttcagcca ttcaaaattc ttcggacttg cagtcgcgta gacttctagg
     4261 ccagcccaat gcgctagttg attcaaaatc gaaccgacgc ccccagctcc gttgatcact
     4321 aagatttttt tcccgcggtt ggcattttct tctggagtca gaccaaattt ttcaaataac
     4381 aattcataag cggtcaatga ggtcaacggc aaggctgcgg cttcttcatc tgaaagattt
     4441 tttggtgcaa gagcgacaat acgttcgtca accagttggt attcttgatt gcttcctgct
     4501 cttgtcgttg tacctgcgta aaaaacgcga tcgccgacag aaaatttttg cacttcatca
     4561 cctacagcaa caactttccc gactccatca aaccctaaga tacgtaaagc atttcttatc
     4621 ccgttatctt ggcgaagttt cgtatcgaca ggattaacag atactgcgtt gacttttacc
     4681 agaagatccc gtgaactggg tacaggtatc tgttcttttt catcaataaa gctttttgga
     4741 tcatcgatcg acaacccttc ataaaaaccg actaccttca ttaattcctt ttctgacata
     4801 cgaattcctc cttagcttca tcaagttgga gcatccatac agttttcatt tcaatatata
     4861 tagtatgcta tttgttccac ttttattcat cataattatt ctcttactta aagtaaacta
     4921 tttgcacttt tttggttaaa aaagaggttg cgacaagact tttgtcacaa cctcttttaa
     4981 acggtgttca atcaactgac cctcggtatt cgctcaaaaa acgacaaaat atgaggagct
     5041 atttttgttt ggctgttctt attacttgat gcaggacagc tttttcacga cctctaattc
     5101 acggtgttca agcaagaaat cattgttcac tgaaaagcgt tggatgttct ttttcaaagg
     5161 tgtctaagat ctctcgtact tcatcagtag atttcgtatt catcaactga actcgcaagt
     5221 catttgctcc tggaaatcct ttgatataga ttttaaagaa acgatgtagt cctgtgatgg
     5281 aacgcgggat gatttctgaa taatgatctt gcagatctag ttgcagacga agtagtccga
     5341 tcagttcttt ttctgtatgt tccctcggtt ctttttcaaa tgcataagga ttcttgaaaa
     5401 tcccccggcc aatcatgatg ccatctacgc catatttttc ggccaattct tctcctgtct
     5461 ggcgatctgg gatatctccg ttgatcgtga tcagtgtttg cggagcgatt tggtcacgta
     5521 aagcaatgat tttagggatg acttcccaat gtgcatctac tttactcatc tctttacgcg
     5581 tacgcaaatg gacagaaaga ttggctatat cttgatgaag taggtgactg atccattctt
     5641 ccatctcaga catttccgta tagccgatac gcgtcttgac gctgacaggc aaaccgccag
     5701 ctttagccgc ttcaatcaat tgtgccgcta cttctgggcg aaggatcaag ccactgcctt
     5761 ttccacgttc tgctacatta gggactgggc agcccatgtt gatatcgatt ccttggaagc
     5821 ccatttctgc catagcaatg ctcatttctc taaaaaattc tggcttgtct ccccaaatat
     5881 gggcaaccat cggctgttca tcttctgtaa aggtcaatct tcctcgtaca ctgtcttttc
     5941 catctggatg acagaaactg tctgagtttg taaactcagt aaagaagaca tccggtgctg
     6001 ctgcatgctt tacgacatgg cgaaatacaa catctgttac atcttccatt ggtgctaaaa
     6061 caaaaaaagg ctttggcagc tctttccaaa aattagtagt catttatttg ttactctcca
     6121 ttctatcaag cagtgaagtc gcaatctttc tcaaaaacat tgtcattata ctaggaaaca
     6181 aaaaaagaag caaagtgaac agcttaaaag atgtttctga ttgagcaaaa aaagactgtg
     6241 acaaaaatct tatcacaatt ccttttccgg aataaatggt gttcaaaagc ttgcatctgc
     6301 ggtaataagt ccaaccaaac gaaaaatagg aggagctatt tttgtttggc tgttcttatt
     6361 acttgatgca gaacagcttt ttcacaacct ctccactaaa cggtgttcaa tcagctgacc
     6421 ctcggtatta gctcaacaaa cgacaaaata tgaggaacta ttttcccgtt tgtttctcta
     6481 atactcaggt caaaacgctg atttcacaac ctcttttcta acttctcaat cctcttcctt
     6541 tattgatcaa ataccaagta acggcgtaca aaacaacgat gaatagcaca agtacgatca
     6601 tcgatgttgc tactggcaca tcgattgttc caaggaatcc gtaacggaaa ccggagatca
     6661 tataaacgat cggattgact ttcgaaacag cttgccaaaa tggcggcaac atagagatgg
     6721 catagaaaac gccacctaga taagttaacg gctgcaagac aaatgtaggc acgatcgata
     6781 catcgtcata agactgagca aaaatcccgt tgagcagtcc tgctaacgaa aacaagattg
     6841 ccgtcatcaa taatgtaatg atgaccatga accatgaata cacatgaagt ggtacaaaga
     6901 acaaggaaat gatcgttacg agagagccga cgagaatact gcgacccact ccgccaatca
     6961 caaagcccca aatgatcaca tgcgtaggaa caggcgctac caagatttct tcgatgtttt
     7021 tctggaattt ttgagagaag aaggacgaag aaacgttggc atacgaacta gtgatagcag
     7081 acatcataat caaaccaggc acgatgaatt ccatatatga aaaaccaccc atgtcaccga
     7141 tccgaccacc gatcattttt ccgaaaataa caaagtacaa ggatgtcgtg atcacaggcg
     7201 gaaccagtgt ttggacccag atacgcaaat agcggttcgt ttccttgata gctaaacttt
     7261 ttaatgcagt aaaataaaga ttaaacattt ttctcctccg tatgattatt ttcttctgtg
     7321 attttcaaaa acagttcttc taaacgattc gacttattcc tcatagaaag aactttgatt
     7381 ccttgtcttg tcagctgatc gaagattccg ttcacccctt gatttcgttc aacttcgaca
     7441 gcgattgtct gttcatcttc aaatgtacaa gcgtatcctt caatcacagg tttggtctca
     7501 tatggcgcta aatcaaagat aaatgtttca aattgcagtt tggatagaag ctctttcata
     7561 ctagtattct cgatcagttc acctgattgg atgatcccga tgttgcgaca aagcatttca
     7621 gcttcttcta aataatgcgt cgtcaaaatg atcgttgtac cattttcgtt caattctcgg
     7681 agaaactccc acatctcgcg tctcaattca atatcgactc ctgctgttgg ttcatccaag
     7741 atcagcaaac gtggttcatg catcaatgcc ctagcaatca tcagtcttcg cttcattccc
     7801 ccggaaagca tacgtgcacg gacatttctc ttttcccaaa gattggactg cttcaaatat
     7861 ttttcgctgc gtttcatcgc ttctttgcga gagacaccgt agtagcctgc ttgattcacg
     7921 acgatttgct ggaccgtttc aaagggattg aaattgaact cttgcgggac taatccaatc
     7981 tgttgctttg ctcggactag atccgtatcc agatcataac cgaagacatt cacttttcca
     8041 gatgttttat tgactaaaga agtaatgatt ccgatcgttg tagacttccc agctccattt
     8101 ggtcctaata gcgcatagaa atctccttct ttcacggaaa gatcgattcc tcgcaatgct
     8161 tcgaccccac ttccataaac ctttttcaaa ttatttattt caagcgcatt tgccatttat
     8221 ttcctatctc cttatagaca tgattcttta ttgtatcatg gaatacagca gatgaaaaaa
     8281 caacttggtt ccagcgaaaa atcaggttgc tgggctcagg aaaatctcct aatgaaagac
     8341 atcaatcgga cgaagtcaga caaccttttc agaaaaaaca aatcgaagat aaaggattac
     8401 tattcatatg tgcaacaaaa ggagcgtgag acaaaagtaa agaacacttt tgtctcacgc
     8461 tctaaacacg tataaacggc ggaagcaaaa gccaagattt ttgatccaat aaatactttc
     8521 gatcacttcg cctatcccaa tacatgccac tgccgctttt catcaaataa cgcggcttcc
     8581 gtttttgcgg tttctactaa agtctctggg taatccaaaa ctttcagcaa ggcaatcgca
     8641 tttcgggtcc ttgaaggtcc ttcttttaat ttgaaatcaa aactgattcc ttttccttct
     8701 gttacttgtt cttcaaagtg gacattctca caataattct ttaaaatctc tgtcaattcg
     8761 atatcatgtg tggcgacaaa tgctaaagat ggatactccg caagccaccg aacgactgaa
     8821 gcggaagaag caatccgttc gatcgtattt gtccctttta aaatctcatc tacgaagcag
     8881 tagcagcgtt ctttggaagc cacttggtcc aacaaacgtt tgatcgattt gatttctgaa
     8941 acaaaataac tgtcgccttc aaataaatca tcttcgattg ccatggaagt caacacatgt
     9001 ccaggttgca gggtaaactg ttctgcaatc gctgtttgga tcgtctgtgc tagaatacag
     9061 ctgatggcta tacttttgac atacgtagat tttcctgatg cattggaacc cgtgactaat
     9121 gtattcttct gccagttgac cggattcact accgcttctt ttaacaatgg atgatagata
     9181 tctttcgctg tcacaccgcc ctcagtaaac tctggtcggc aagtgatggg catataagta
     9241 cggtaattca atattgcagc ggctacttct aattctccca gcaattccca cagcttgatg
     9301 gcttcagttg aatgcttttc taatttagtg agaacgaaat gataggagat aaatggcagc
     9361 atgaacatga tgttgacata ttcaaaaatc aactctgctt cactcccact cttcatacga
     9421 aatgaaaaac ctaatttagg gatgtctctt aatggtttag tttgctgttt cagctcttct
     9481 tgaagtggtg tcggcagttt tgctagctga ttggcggctg aaatcgttga aaccaaataa
     9541 ctcatgctat tcagctctgt ttctagtgtt tgtttcttca ctagataata gaccgtattg
     9601 aatactaacc caccgattgc caaatagatt cctagtactt gtcctaaaag aagcaaaccc
     9661 aaacccacca gcggaagcaa gccaagaaaa ataaataaac ctacgcgtcc aatcttttta
     9721 ctttttccat ccgctagata ttgttttgca aaattatgat ctttttttcc taaacgagcg
     9781 aattgatatt ggattttttc tctcagttga ggattttcct gataaaaagc aatcagtttt
     9841 tctaactgct gatcttcacc aaaatcaaag ctgcgtaaac gttgatacaa cgcttctgaa
     9901 cctacactag aataggtact gttgattcct tcgaatactt cgaacatatc caggtcatac
     9961 caagtaagat catcgatttc gctatcccat gaacggaact tcttctccgt gagccatgct
    10021 tccttcaagc tttcttcttt atcaaatctc ggttgataag gaattttttc ccatttgcta
    10081 cgaactgttt cctttaattt caaacgtgaa aaaatgtcta aagcaactaa taaaataaca
    10141 gctgcaataa taattaaaac gataaattct ctttgaccca tatccctctc cgttccttta
    10201 tttgttagct ttagtatagc actgttgcgc tatttgaaaa gagggtaaaa aaacaccttt
    10261 caaaataaaa ggtgtttttt tcttgtatat tacatattta tctctcattt atcaaaattg
    10321 acaaataatc atagggagga attgttgtta attcttgttt attctccagt gcattgacaa
    10381 ctgtcccaag ggaaaggata gcctcaggta ctattttttc ttgttctgtt ggattagcta
    10441 agcgaagaac aaatccttgt tcatgaaaat aagaaggata aatcgatgat acaattaagt
    10501 cttcaggtaa ttcaatcaat gaaaatttac gaggaacatc aagttcattt tgtaatggcc
    10561 aaatcttgtt atctaacctg tgaataaata cattcagttt ctgcttttga taagaaatag
    10621 attgttctaa ccgtttttct gcctgttttg ccactttgaa ctcattaaat cttccttctt
    10681 ctacattaaa tccaaaagtg actttatact cacctatctc ttgagctagc ggtgtttcca
    10741 tcatgacatg cccttcattt gtcgtatctc ctgaagctcg cccaggtcgc caagcaagat
    10801 taggttttcc aagttcccct gttgttgaaa ataaggttat gattaattga ttttctatac
    10861 gttcatactc ttttaaacca tctgcataaa acgtaaaata atgatcattt tctgccacag
    10921 agacactctt atcgaatatt tctaaattaa caggtttctc taccatagtt tctgaccaat
    10981 tcggaggaat gacatcaggg gtattttcaa taaatccgtt ttgaatttgt gcaattgatt
    11041 tttcagattt atctaaaaca ttaattgcta agcgcatacg atgcgagtaa acagtattat
    11101 gaattttgag tgtgccttca acgatttgac tttctcccag actgaacgat atttcataag
    11161 atatttcttc catttcgggt ttttcagata aacgatcttt taaatcttta ggaagttgag
    11221 cttttccgta aacaactaat gtttcttgta aagaatcttt atatacttta gctgtttcta
    11281 attttagaga tagttctgta tctccttcta atggtgaata atcatacgta tcgccatcat
    11341 ttgctgaatc gatcaaatga acgaaatcaa gatattgtct atctccaact atcaaattca
    11401 attgcccatc tttaaaagaa agtttatatt tattgttact tatctcagtt ttgtctatct
    11461 cgattgctgt ttctaatttt tcattatttt tctcaaattc aacaacagtg tatcctagtg
    11521 ctggcacatt tacatttatt tttatgtcaa gttcataata agctggttca tcaaaatatt
    11581 cgaaacctgt agcaatcatc cgataagcat ttttccgttc tggaaaataa acttcttttt
    11641 caatgatatt atagggacta cctttgaagt gtattttctt tgaatcagct actacatgca
    11701 cgattttcga tccactataa ttctcagtcg ttgtgttgaa aacaaggact tcattcgatt
    11761 ttagctttaa tgcatcagcc atccgtttta caataaggtt ttcgattccg tcagctatct
    11821 cattagcttc tttgattcga tggaaaatat cttccgcaac attatctgaa acacagccac
    11881 caatactatc atgagcctgg ttttgcaaaa tctttttcca taaccgacga agtaggcctt
    11941 tactgatatg gataccattt ctttctgcaa tcagcatgag cggctcaact cgttttagaa
    12001 ttttttgttc caatttaaaa ttttctattt tcatttttgt tcttactgag ccaatagaac
    12061 gatgaacacg agagtaaacc ggctgacgaa gttcaccttt atattcaggt aattcttttt
    12121 tgtttctgat aacttctaca taagaaggat aatctgaaat aatattgtgg aatttactgt
    12181 atttattcaa cttctctacg acttgatcaa aatttaaaat cattgacttt tgatctacac
    12241 ccgaaggtac taaaatatct ttgttttttc cttgcatatg aagcatttca actgcttcat
    12301 ccaacttttc ttttgcaaat tcaggaatat ctttaattgc gtctaaggtc atcattccag
    12361 tcatataacc aaatggaaaa ttcatggcat aaacaaattt atctcccaat cctttccaga
    12421 taaaataagg agatggaacc atcgtttcaa agtttaatcc tcgccaacac atgaaattat
    12481 cgatttctac ttgatgcaaa agtgtaggca aattcgcatt gaacccaaac gtgtcaggca
    12541 aataccctac catcattgga tctccatatt taaggatcgt atcactgatt ccaatcatta
    12601 agtttcttaa aattgattca gcatccacta ataaggcatc tgtttgtgca taccacggtc
    12661 ccacgaatag ttgtcctgtt ttcatcagtc gttggataac cttaagcttt tgcggattga
    12721 tctccaaata ctcatcgacg attgaagttt gtccatctaa acaaaagttt actttagggt
    12781 tcctttccaa ttcatctaga acttcagtaa agacctgatc agacaatacg atggcatctt
    12841 catttgtaaa ataccattct ctgtcccagt gcgtgtgttg aattatatgt gcatttacca
    12901 tatcagattc tcctactttc cttcttctac tgaatttact tgttgttctt ttttatactt
    12961 gatggatttt agtgcattga ccattaacgc tgtaaccact cccccaataa caattgatac
    13021 taaatataaa ggtatattcg taacaaaagg aattgccata aaggttgaaa gcattgtttc
    13081 ttgagtaaca ccaaaaaatc catttagacc acatgctact gcagtgccca ccacgatgct
    13141 tggtattact cgtaaaggat ctttcacggc aaacgggatc gtcccttcag taatccccac
    13201 aaggcccata atcatagctg tctttgcatt atctcgttcc gattcatcat aaagtttttt
    13261 gtttaaaatt gttgctaaaa acattccaaa tggcggaata gcaattccta ataacatggc
    13321 tgtactaggt ccaaagcttc cgcttgaaaa agaagcaacc ccaaaagcat atgcgatttt
    13381 attgataggt cctcccatat caaacgccat cattcctcct aaaatagccg acatcaaagc
    13441 agcatttgct cctgacatgc cttctagcca attagtcagt cctgttgtca ttgcttgaat
    13501 aggcgtagct agtacatacc ataatatgat tccaacaact aatgtagtaa caaaaggaat
    13561 cagcataatt gattttagac taataacttg catcggtact tttacttttt tcaatgcatt
    13621 agctaaatag ccacaagcta atccagcaac cattcctcca ataaatccca taccaagatt
    13681 ttgacacatc atcccagtga taaaagctgg tgctagaccg ggacggtcag caattgaata
    13741 tgcgatatag gctgaaatca tcggtacgat aaactgtaat cccgtctgac caatcattcg
    13801 caggactccc cagaatgtat ccgtctggtc ccaaacacct gtgccaccaa aaatgactgc
    13861 aatagaagtt agtaaccctg ctgctacgac aagcgggatc atataggaaa ccccactttg
    13921 gaaatgattt agaatttctc ttccttgttt cttgaattcc attagtaact cccccacgat
    13981 atatttttta tttgttaatt aatgcaattg cttcatcaat cgtctcttca ccatctttta
    14041 ctacctctga ggtccctact gttaaaatcg gtttaccgtc aaagcgacct cgttctgaaa
    14101 catttgtatc tactgcaaat atgacaacat ctgcctcgga aacatccgaa attgaaagtt
    14161 tgttttctat ccccatagcc ccctgtgttt ctactttaat atctaaccct ttcttcttcg
    14221 caaacttttc taaattagct tttgccatat aagtatgggc aatccctgcg atacatgctg
    14281 ttactgctac tatttttgtc atatgaaatt cctcctttta aaatgataga gcttttacca
    14341 attctttttt atttctcgaa tcttttattc ttttaacaaa ctcgtcatcc atcaatcttc
    14401 cagataattc tgctaaaata cgtaaatgat tagtaccttt atcagtgtct tttattgcta
    14461 acaaaaagac gagatcaact ggttgatcat ctaatgcgcc ccattcaaca gaccgcttta
    14521 acttggctac agccactgtt gattcaatta ctgctaggct tttgccatga ggaatggcga
    14581 tatttttccc aattcctgtg gtcatatggt tttctctgtc aaatacgtct ttttgaaatt
    14641 gtttgtggtc agacacaaac ccattagtct ctaacaattt cgataatttt tcgactacac
    14701 tttttttatc agatgcattt aaatctatca ctatattatt tatattgatt ttttcactga
    14761 tcatttataa gccttctttc aatatataaa ctaatttttc aaacgacgaa ctcgttttta
    14821 aatttgctaa taactcatga ttcgaagcga tttcattgaa tgtttcataa attttattca
    14881 attcaggttt tactttttca ttaagtgcaa aaaagaaaac agaatgaaca agtgtcccat
    14941 cccaatcaat tccttctgga ttaacgtaga cacagattac tggctttttt ataaaatcta
    15001 cttcgatatg cggcatagca aaatcattca tcgctgttga agatagtttt tctcttttga
    15061 ttgctccctc tagtatccct tctttcgcgt aattttcttt taccaatttt ttggtaatta
    15121 tatttaaaac gttcacttga ttatccttta tttggtgggt aaaaaagcaa aatcgtttat
    15181 ctaataaatt tagaaaagca tcttctgttt gttcatttaa ctgtagatac tgttttactt
    15241 ttctgacatc tgcccctaac atcaaaggac taacaacaat aacaggaaaa tttgtatttt
    15301 caatcggaat cgtgcttata acaagttcat cagtgacttt tctatcttgt agttcattga
    15361 tgcttagtac atcttctatc tcaaccatat ctgcaaaacc gttatttatt ctttgctcta
    15421 ataattttga tgttccatat ccgctactac aaactaatat cactttcgtt ttgtcaggtt
    15481 tgtaacgatc caaaagtgat tgaatatgga gtgttacgta agcgatttca tcttcattaa
    15541 aatgtataca gaatctctct tcgatttcct ctaataaatc tacacttatc ataaatgacc
    15601 ttttaaaaga atatttgatt ttgtccgtat aagggttata gatatttact cccaatttga
    15661 gtcttttaac tgctgcattg agatgtaata ctagattatt gatcaattct tcatcaggat
    15721 aaaattcatt taatttaatt cttactaact cttgtagctc aacacccata tctaaatagg
    15781 aaagcttcgt ttgatctaag tcgtgattca tagcattttt ttcaatcgaa gtaatgtgaa
    15841 tgtcaacata agcttgttca tctattggca caataattga aaatgctttt tcaatcattt
    15901 tgattaaaat aagtgtattc tttgagattt cttcttcaga attattacta cgtgataaga
    15961 aattgcttgt ttcaatcctt tctatcgcaa tagcaaggtg gatagctaaa gtttgaaatt
    16021 cataatctgt aaatttcaat tgtgtcttat tcatgtatcg attaagcaat tcggctactt
    16081 ttttcaatat gtccttattt aagaaattat tcgcttcacc aatgaaattg atattcaagt
    16141 ctaagagatc tgttttactg ttagatgctg taaagccacc ccaataatag ttcacaactt
    16201 ctgacatcaa ccttctttta cttttttcat cagctttgat gcgcacgcca tttcgatctg
    16261 aaactaagga catcccatgc ttgtttagtc tttttctaac atctttcatt gtgctttcta
    16321 ccgtcgacct gctcacgtat aattcgtcag caagattttg aatctttaga gaatcattag
    16381 tattcaataa tttagcaaac acataagtta gacggtcctc cggagagtct gcgatattga
    16441 ttttatgact ggataataaa tttcgaagtg tttctttttt tccaataagc gaaacgcctt
    16501 ttttaggttg aaccaacatt tgtacatctg gcattttttg gattatttct ttcaaaatta
    16561 aaatatcttt gctaattgtt ttcgtagata catataattg ttctgataat tctttgtaag
    16621 ttgtgtgctc attttcaatc aaaaagtata gtattttcat ttgccgttcg ctaaattcga
    16681 tgcccattat cttcacctcc tgaatacagc ttaagtgtac aattattatc tctattttca
    16741 tacagaagaa agttcttgat ta
//
LOCUS       DADVXU010000059        16692 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.062, whole genome shotgun sequence.
ACCESSION   DADVXU010000059 DADVXU010000000
VERSION     DADVXU010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.062"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            127..270
                     /locus_tag="KB953_001812"
     CDS             127..270
                     /locus_tag="KB953_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HBB6866785.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            848..2431
                     /locus_tag="KB953_001813"
     CDS             848..2431
                     /locus_tag="KB953_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBB6866786.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIVLVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(2868..4052)
                     /locus_tag="KB953_001814"
     CDS             complement(2868..4052)
                     /locus_tag="KB953_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBB6866787.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(4077..5084)
                     /locus_tag="KB953_001815"
     CDS             complement(4077..5084)
                     /locus_tag="KB953_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBB6866788.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(5256..5615)
                     /locus_tag="KB953_001816"
     CDS             complement(5256..5615)
                     /locus_tag="KB953_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866789.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(5587..5958)
                     /locus_tag="KB953_001817"
     CDS             complement(5587..5958)
                     /locus_tag="KB953_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HBB6866790.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            complement(5975..6277)
                     /locus_tag="KB953_001818"
     CDS             complement(5975..6277)
                     /locus_tag="KB953_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866791.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            complement(6294..6764)
                     /locus_tag="KB953_001819"
     CDS             complement(6294..6764)
                     /locus_tag="KB953_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HBB6866792.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(6739..7017)
                     /locus_tag="KB953_001820"
     CDS             complement(6739..7017)
                     /locus_tag="KB953_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBB6866793.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(7014..8063)
                     /locus_tag="KB953_001821"
     CDS             complement(7014..8063)
                     /locus_tag="KB953_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HBB6866794.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(8020..8949)
                     /gene="tadA"
                     /locus_tag="KB953_001822"
     CDS             complement(8020..8949)
                     /gene="tadA"
                     /locus_tag="KB953_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HBB6866795.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            9081..10835
                     /gene="ade"
                     /locus_tag="KB953_001823"
     CDS             9081..10835
                     /gene="ade"
                     /locus_tag="KB953_001823"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBB6866796.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNNQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(10933..11595)
                     /gene="trmB"
                     /locus_tag="KB953_001824"
     CDS             complement(10933..11595)
                     /gene="trmB"
                     /locus_tag="KB953_001824"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBB6866797.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(11665..12447)
                     /locus_tag="KB953_001825"
     CDS             complement(11665..12447)
                     /locus_tag="KB953_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HBB6866798.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(12651..13862)
                     /locus_tag="KB953_001826"
     CDS             complement(12651..13862)
                     /locus_tag="KB953_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6866799.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(13855..14592)
                     /locus_tag="KB953_001827"
     CDS             complement(13855..14592)
                     /locus_tag="KB953_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6866800.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            14730..15164
                     /locus_tag="KB953_001828"
     CDS             14730..15164
                     /locus_tag="KB953_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HBB6866801.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            15157..15501
                     /locus_tag="KB953_001829"
     CDS             15157..15501
                     /locus_tag="KB953_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBB6866802.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            15681..16691
                     /locus_tag="KB953_001830"
     CDS             15681..16691
                     /locus_tag="KB953_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBB6866803.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
BASE COUNT         5323 a         3417 c         2940 g         5012 t
ORIGIN      
        1 tttaaaataa agggaaaaca gtcaatgagt tcaagtaaaa taaaaaaaag tgaacaccag
       61 ggcgattagt cgccctggtg tggtttaaaa cgacctaagc atgccaaagc ttattggttc
      121 gtcaatgtgg aagtttccga tgaatctgat acgaaaaaat atgaagtatc gatggcggat
      181 accgatttta tcgccaaact aaaattttat ttcgggttcg ggtaccgcga gaaggcaagt
      241 gctacattcg gtaaatcatt caagaattga tgaaaggcta ccgcttgcca ttgcaaccac
      301 aacgatactc ctgaacgcga tgttggtgag atttccaaag agaagagctg tttaatgcaa
      361 cggtattaag caaatggcaa aaggaagtca tgtagacaaa gttttttatc aaacaccata
      421 tgatttcgcc tgaacgctgg tttagctcag aatacaatga tgtggtccac gagacattac
      481 cctcagtcag ataaaaaaag ctctctgaca gaattacaca tcgataaata ttaagtaaat
      541 gtcagcgcta gttgaacttt ctgacacttt caggtacact ggaaaagaca actacacgaa
      601 ggagggaaaa tactatgatg cgcatgacta aaataacaag taatgaaaat taccaacgat
      661 accatatcgc atttagccat gcaattggtt ttacagttgc tgttgacggg acaagtttgt
      721 ccaaaacagt tgaattcacg taaaatcaat ggccatctca aggtattttt ctcatgataa
      781 tctgagttta aataacacag gccatggata accatggcct ttttgagtgg tctcaaggag
      841 agagatcatg atcatacaag caatttctat taaaaaaaca atcggcggga atccattatt
      901 tgaggattta tcctgtcaaa ttgaagctgg tgaaaaaatt gtcttagtcg gtatcaacgg
      961 aactggcaaa tcaacctttt tacaaatctt gaccggcaga gaaggtgtcg acagcggtgt
     1021 tatcagtcgc aaaaaaggct taaagatcgg gacggtcgaa caagaactta cagtttccga
     1081 agcgaccgtc aaccactact tgctccattc tgccgctgag atacaggacc tcaaacagca
     1141 aatgagtcgc tacgaagcat tgatgacaga accagataca aacttagaga agtgtctggc
     1201 ccgttacggt gagctgcagc atcgctttga agaactaggc ggttacgtat tagaagaccg
     1261 gattgcagcg atcttgcaag gcttagggat tccacaccgt caggaagcac ggttaacaga
     1321 acttagcgga ggcgaacgag ttaaagtcgc tttagccaaa attttagcag ccgatgccga
     1381 tttactttta ctcgatgaac cgaccaatca tttagatctt aacagtattc gctggttgga
     1441 aaactattta cagaatacaa aaaaatcggt tctggtcgtt tctcatgatc ggcaatttct
     1501 cgatcaagtc accacaaaaa catgggaact ggaagaaggt gcattgattg agtatcccgg
     1561 aaactacagc cgttttcgag tcttgaaaga agctcgcttg gctgagctga caaaaaatta
     1621 cgaactgcag caaaaagaag tccagcggtt gaaagtcatg attcgtcgtt ttcgtcagtg
     1681 ggcacatgaa ggagacaatg agtccttctt caaaaaagcc aaagaactgg aacgccgcct
     1741 ggcgaaactg acactcgtca aaccgccacc tcccccgaaa aatcggctgc aaagcttgtc
     1801 taatggtgga aaatctggca aagaagtttt catcattcaa aacttgcacc agcagtacgc
     1861 tgatcaagta ctattcaaag acagcagttt tgcagtctat cgtggagatc atttagccat
     1921 catcggcgac aatggtgcag gaaaatcaac cttactcaaa ttattactcg gtgaggaaaa
     1981 gccttacggt ggcaccatca aattaggtag tagcttgcaa atcggttatt tgccgcagca
     2041 gctgcagttt gccgatcccg atagccgttt gctggcttac gccatcacat tgactggaaa
     2101 cgaagaagtc gctcgacggc aattagccaa aagtggcttt tatcaaagcg atgtgggtaa
     2161 acggatcaaa gatttatccg gtggtgaaaa aatccgtttg gcgttgctga aactcttttt
     2221 agaaaaaatc aacgtcctga ttttggatga accaaccaat cacctcgaca gctatgctcg
     2281 agaagaaatt gaagaaatgc tgcaagacta tcaaggaacg ctcctagcag tcagccatga
     2341 ccgctacttt ttgcagcaac acttccaaga agcactcgtt attgatcagg agcagatcag
     2401 ccgccagccg ctcggccaac tgattgacta ggacgttttt tcgcaacaaa aaacttgatt
     2461 ttcatacctg tgctttcacg attcaggcaa agtacaggtg gaaaatcaag tttttattat
     2521 tcgatcaatg aatgtttcgg atcggttttc tagctaaaca taaagcgaag aataaagtcg
     2581 acagcccacg aggctgatct aaaaatgttt tttcttttta catatgtaat gtcccttata
     2641 aagatgaatg aaaataaaat attgggattt tcctgaaaat caataaataa aatgccagtc
     2701 ttttcccttt tgaccacttt tccaagcaaa attccctaag aagatcctgt aaaaaagaag
     2761 ggaaattgaa gttaaattac cattttgcgg tcttctccgc aaaaaaaaca catgcaagaa
     2821 ttccctcttg catgcgtttc atcttttatc ctaacgaatg ctttggctta gccgcgcaga
     2881 cgctccacat cacgggcaat cattagttcc tcatctgttg ggatcactaa tactcttact
     2941 tttgattctt ttgtagagat atctacttct tcaccacgca attcattcaa cgctggatcg
     3001 atttcacaac caaaccaaga catacctttg atgatttcac tacgtacatg tccgtcattc
     3061 tcgccgatac cagcagtaaa gacaattgca tcaacaccat tcatcgttgt cacataaccg
     3121 ccgatgtatt ttcggatgcg gtccacgaaa atgtcataag ctaaacgaat atgttctttt
     3181 tcatagttct tttcaagatc acgcatatca ctagacaatc ctgttaggcc tagcagacca
     3241 gatttcttgt tcaaaacatt gatcatttca tcaatatcaa tctctaattt ttccattaaa
     3301 tatgggagaa cagctggatc gatatcacct gaacgtgtac ccattgtcac cccagctaat
     3361 ggtgtgaatc ccatagaagt atccatggat ttacctccat cgactgctgt gatcgataca
     3421 ccatttccta agtggcaagt gatgattttt agttcttcga tcggtttgtt caataatttt
     3481 gccgcacgtt ccgacacata acgatgactt gtgccatgga agccatattt tcttactttg
     3541 aaatcttcat aataagacac cggtaagcta tataaataat tttgctttgg cattgttgag
     3601 tggaaagatg tatcgaatac agccacactc aaaatgtcag gtaaaatctt tttaaacgct
     3661 ttgatcccca ttaagtttgc aggattatgc aaaggagcaa attctgctaa ttcagcaatg
     3721 cggtccatta ctttctcatc gatcactact gaatccccat aagtttctcc tccgtgaaca
     3781 acacggtgcc ctactccggt gatttcatca taagagctta aaatttgcag ttcaatcaac
     3841 tgatctaaca gcatttttac tgctacttcg tggttttcga tatccatgat ttgttcgaat
     3901 ttttgattat cgccgtactt gatggtaaaa atagaatctt gtaaaccgat acgttcgacg
     3961 atccctttcg caactacggt ttcttctggc atttgatata attgccattt caaacttgaa
     4021 ctcccagcat tgattgcaat tgttttagac atatgactct ccttctcgtt gaatgattac
     4081 agagcagatg atttccagtt tttaaactcg ttgaaaaatt ccgtcacctt ctctggttct
     4141 tttaatgaag caagtttcac aagcaagact tcttttactt gttgtgcttg tggtcctttt
     4201 ttctgtaaga tcaaaatact tttttgcgat tgtttattac ggaacaattc atctggcaat
     4261 tgaatcattc cttgaagata accttcttct ttaaaccatt ttttgatttc ttcactttga
     4321 tccgtttcta aaaatccact cggcatcaaa aacaaaccaa aaccatccgg cttcacatat
     4381 ttcattgatt gctctagcaa taaatgatgt gcataactat ggccttcttc ggtacttgtt
     4441 aaaaactctt ttgctttttc gtcattcgga taataaccga ttggcaaatc gctgattgca
     4501 aaatcgacag gatctattaa taattcctgc aacccgtctt gatgaaagta ttgaacattt
     4561 gcttgtgtca aatcacttgt agaagctgcg acagccaaca atgtatcatc aatatccaca
     4621 ccaattcctt ctgtttggta ccccgcattc gataggttta aaagaactgt taataacaag
     4681 ttgcccattc ctgccgcaat atccagaatc ttaaccggtg cttttttatt tgtgtatagt
     4741 tgttcaataa gaaaaacaaa taaaaaaccg atactatcag gagtgagttg atggttcgcc
     4801 tgcaaatgtt ctgtctggct gcccttcaat aaaagtaatt gagacagtct gcgccattct
     4861 tctggctcga aagaaagttt cttcaactct tcataaagag ctgtgatccg ttgcgttgtc
     4921 tcttttgtcg gcactccatc caccacacgt acttgataat catctattaa attttctgca
     4981 ttttcgacat aggcttctaa aaaggaagtt cccaatgcat tctgtaaaag ctgaatagct
     5041 tccaggtttt gattaaaccc ttgttccatt ttttctggta acatggtgca cctcatttct
     5101 agatatatta taacaatttt ttcacctgtt taattctatc gaacaattga caaaatttca
     5161 atcgttttca ctgtttttta tttgtgaaac ggcaaacatt tttttattaa aaacagtgtt
     5221 ataacagaaa agagggcgtt ttcacgaaca ttccatcatt ctgtactagt atctgtatca
     5281 ttttttactt cttctgtgaa ccgaaagata aacggtgaga cttccacctc taagaatagg
     5341 gactgttgta cccactgata tgttacagat ccagttgtgt agtaaaaaac tccttccgaa
     5401 ctttcggcaa gcgtagactg ttccgataaa gccatctgct tcattatttt tgctatataa
     5461 taatttttga ttcgtatatt aagatccatt gacaaacggt agctttctaa tgctgccatc
     5521 aataaaaacg tacataaaag aaataaaagc aagacagaaa ataaaataga gcccttataa
     5581 cattttctac tgatcgtttt ttgtccatat ggcatatcca tcttctccat tttcaaagtg
     5641 tacataaaaa gaaattgtgt gttctttttc caataattga agctcagtga tacttgttaa
     5701 cataggttga tgtccgccgg aagttcttct tttacgtatc attgcctgat atctttcgat
     5761 gatatactgt ttatcctcga tcgtgtagtg cagttgctga tttttcacag agacattgtg
     5821 actgctcttg agttcttctt ctaattgtgc taaaaatatt agccactctt tttgatcttt
     5881 acgctgcaaa aattcactta cttggatggt ttgctgaatc aaaagattga atgtgagcaa
     5941 tagactgctc aatatcatca gtccaattag acattcaatc agcgtaaatg ctggaattac
     6001 ttgttttttc aatactgatc gcttctttct ccatattgac aaacactttt tcgattactc
     6061 cattattatc gacaatttgg atatcgtatg ggatcatttc ttcccttcgc tctggtcttt
     6121 tgtgatacat ccgctgcgtc ttgatatctt cataaatcat tcgaataaat agcagttcct
     6181 ccccactttt cttttctgcc tgaagcaaaa acagctggcc aaagctcact aagtagacac
     6241 agacagaagc gatcataagc gctgtaaggg actccaacaa aacaaatcct gttttattca
     6301 attctttttg tatagcgacc actccctatc tggaattgat acgtaatcgt ccgctctttt
     6361 tcttcccaat aaaattgata agcttttagt gaactttcat tcccggttct agcagcaaaa
     6421 gtaatagaag catggcgttt caaggtaatc tcatcaggaa tatctaatac ttcccaattt
     6481 attttatctg gattcggaac atgaaaaatg atttgattct cttcactatt ccaataaaag
     6541 cctgtttgga tctgattcac aatagcgatt ttttgcgtag aatagattcg tttttcaaat
     6601 tggccgaaaa attgatagac tgccaattca ttcttccatg cagaaaatgc gagaactgga
     6661 aaagaaagga tcgttgtaag taataaaatc agtaagagtg attcaaacaa cgtatatcct
     6721 gatgctaaaa ccttaccttc aaggccgctg atagatttct gcatgttctc gtttgatata
     6781 gccttcattc aacaattcct ccacagtagg aactcttcca ttattttgaa gcgcatagag
     6841 ctctttttgt gtgtctacca actggatgat cgcttcacga ctttcttggt ctacatgatt
     6901 cctataacgc gacaaattag ggacgaacaa caacactaat acactgatga tcagtaagac
     6961 gattagcatt tcgatcaatg taaaaccgct ataataagat atttttctct ttttcatggc
     7021 aaaacctcct ctatgcttcc gtaaacaggt aataaaacag cggcataaac taataaaatc
     7081 aaagccgcta cgcccaaaaa aatgattggc tgaataaaat gcatccagtg atgaagacgt
     7141 ttgaaaagct gttttcttgt ccattcgctg taaaaaatca gttctttacc taaacttccc
     7201 tttgcctctc cctgtaaaac aattttactg aattcttctg atagaaaacg ataattctgg
     7261 aactgctcag ccaatgacat acctgcctcc aatccttgaa tcaacttaga ggcaagatgc
     7321 tgaatcagac tttcttgttt cgtttccttt aagcagacta tgatttgcct taattcgaaa
     7381 ccttcatgaa aaagctttcc aatctctagt gacaaataag ccgattgata caaggaatat
     7441 atcggtccaa tcaataattg tctgcttatc cattcactct gatcaacagg agacaacttc
     7501 atcagacgat accttactag taaagccaat agagtaaccg ctaggatgaa tagtagaaca
     7561 taccaatgaa agacttgtat cagctgaata ccccaatgct cttgcatgac cattcctgaa
     7621 ccaataagct gaggcaaaac gaacaaccgc atactaaaca agatacctag caaaaaggtg
     7681 agcaatagca tcggatatgc aagcagcttt ttcaaatcat tgcgaaatcc ttcaacaagc
     7741 cgaaactgtt tggcaatttc caacaaagtt gtcggcagat tcccatgacg ttcagctaac
     7801 tcgatctgta ccagttgctc tgctccatat cctatttgcg aaagtacctc actaaacgct
     7861 tgcccttcct ttagctgatg ttggatattt gccaaaataa caggtggaaa cgaacggatc
     7921 gtgagtaata aataaaggga ttcttgcaga ctaaaaccag ataaaagcag ttctcccatg
     7981 gtttgtgcaa actggttttg ctggcttctt gttaatcctt tagaatagtt ctgactgcca
     8041 aatattttcc tgagcttctt cataactttc ctcccatgat ttttttactg attcttcctc
     8101 cataaattga taactccata aaacagaagg atggtgattg gcatcttcca aaagttcttg
     8161 ataaatgaca ccagctaaac attcagatag ctcttctttt ttctttgtca gttcatggat
     8221 tctgaccaat gtaccttcaa gattgcgtgc atgtacggtt gcaaaaaccc ggtgccctgt
     8281 tagtgctgct cttaccgcag catgtgcagt tgattcatct cttatctcgc caatgattag
     8341 caagtctgga cgatgccgta atgcgagtct caaaagtgta tcataagtct gtgctatttt
     8401 cggattgatc tgtagctgca gaaaaaacgg ttcttctatc tctacaggat cttcgatagt
     8461 aatcacttgc aatttttctt ccaaagccaa ccgatacatt agagaagtct tgcctgatcc
     8521 gactggtccg ctgaaaaggt ataatccacg tctgacggtt ttctgtaaaa tagttgcaaa
     8581 atctcgagaa ctaaagcaac gatggggtgt ttcaaaagta ccgtataata gccgaatcac
     8641 taaactctct cgttgaaggt aatctcctac agaagacaac cgcagacgaa cttcctgttt
     8701 ttctaaagaa taagtaaatg cacctaactg agcttttctt ttttctccga tatccatctg
     8761 tcccaaatac ttaaatcttg agatcagttg ctgtccatct gccgaagaga ctttcttcca
     8821 gaatgttcgt ttttttccat agcgaaaata gatgtaaaat acttctgaag ctggcaatat
     8881 atatacgtct tgggcatgtt tggcaaaact ttcacttatc atttgatccg ataatcgttt
     8941 tatttccatt attctcccct ccacaaaaaa catacgtctt tttgatcttt ttgtttcaaa
     9001 taaaatgaat ttacaaaaaa atattggcaa aaaccgaatt tatgttatac ttataaaaat
     9061 ttatcttggg aggcactcca ttggaaacaa cattaaggaa atatatcgaa gtagcaagta
     9121 aaaaaaagcc tgctgaccta gtgatcaagc atgctaagat cgtcaacgtc tttacgaaag
     9181 aaatcatgga aaaagatgta gcaatctgcg aaggaatgat cgtaggaatc ggtgaatacg
     9241 aaggaaaaag cgtacataat gctaatgggc aatatctagt gccaggattc atcgatggcc
     9301 atgtgcatat cgaaagctca cttttatcac caagcgaatt ttcaaaagta tccttgcttc
     9361 atggagttac tactgtagtg acagatcccc atgaaatagg aaacattgct gggagtacag
     9421 gtcttgagtt tatgatagaa gatgcaagac aaacactcat gaatatcttt tttatgctac
     9481 cttcttgtgt acctgtaacc ccttttgaga ccaatggtgc aaatttaaat gcaaaaaaat
     9541 tagccccttt tttacaaaaa cccgaagttt taggcttagc tgaagtcatg aactatcaag
     9601 cagtagcaaa taatgaaaca gacatcctag aaaagatcca attgatgcac cagcataaga
     9661 aaaaaattga cgggcatgca gctggtatcg gcatggaaga gttaaatgtt tatcctgcag
     9721 caggtattcg gacggatcat gaagctacaa ctgcaaaaga agctaaagaa cgtttggatc
     9781 ttggtatgta tttaatggtt cgagaaggga ccgttgcaaa agatctggca tcgctactac
     9841 cggcgatcac acctgaaaat tcaagaagat gcttctttgt caccgatgat aaactgatca
     9901 atgatttagt aaaagaagga tcaatcgatc atatcgtccg tcaagctatt gcacttggaa
     9961 tcgatccatt acaagccatt cagatggcaa cattgaatgc agcagaatgt tttgatttga
    10021 aaacactagg tgcgattgcc cctgggtatc aagctgattt cttcttaaca gacgatctta
    10081 ccaagctgcc aattcatgaa gttttctata agggaacatt agttgttcaa tctgggcaac
    10141 tccaagacca tttatttgct gatcattcaa attcatatac gtgttgtctg ccaaaactaa
    10201 acgctcaacc attaaatcca cattcgttag agctgcttct ttcttcttca caagcccatg
    10261 tgatcgaaat tatcccgaat agtctagtga ctaaagattt ggtagaaaaa gtggaccgac
    10321 aaggcgaacg attcattcct tcaatcgaaa aagaccaatt aaagattgcg gtgatcgagc
    10381 gtcaccatca aacgggaaac attggattag gcatagtgaa aggatttcag ctgaaacaag
    10441 gagcgatagc gacaacgatt gcccatgact cgcataatct agttgtcgtt gggacaaacg
    10501 acgaggatat gctgtatgct gctaatcttt tgattcaaaa aggtggcggg atgaccgttg
    10561 tcaacaatca gcaggaaata gcttgtgttt ctcttcctat aggcggaatc atgacacagg
    10621 aacctttttc gatcgtctat gaacagttga atgaattaat aactaaagca catcaactag
    10681 gagcagctaa aacctttgat ccttttttga cactttcttt cttaacatta tctgtcatac
    10741 ccgaattgaa gatcaccgac aaaggattat tttcattttc tcgcttccaa ttgattccac
    10801 cttgtggaaa aaccgatgaa ataaatacat tttaaatcaa ataaatccca gaaaatacat
    10861 tacatctgta atgttcattt tctgggattt atttatggat attaatgttt ttatgaaagc
    10921 agaaagttag tttcacagac ttttcttttt ggtcttatct tgaaattgag cttctactcg
    10981 ataaatacgt tgaccttttg aagaaaattt ttcttcatac tccgtcataa tattgccttc
    11041 aaattgactt tgatgtaaat ctaaccatac ctgtttgagg atcatgccat attgagaaaa
    11101 acttgccaga gaatactcaa acaatccttg attatctgtt ttgaaatgga tttctccatt
    11161 tggacgaaga atctgttcat ctacagcaag aaaagtctta tatgtcaatc gccgtttttc
    11221 atgacgtttt tttggccatg gatcggaaaa attcagatag atttgatcta cttcgctgtc
    11281 agcgaaatac tctgtcaacg ctgatccatc cacatgtaga agtttcaggt taggaagcgg
    11341 ttgttcgatc aacttgtcta gtgcaatcga aacaacactg acctgcattt ctactccaat
    11401 ataattgatt tcaggatggg cttttgccat tccagtgatg aactggcctt tccccatacc
    11461 aatctcaatg tggattggat gatcgttttc aaacaattca ttccattttc ccttccacaa
    11521 tgttggatca ctaattacaa aattcgggtg agccgctaac atttccgctg ctcccggacg
    11581 atttcggaca cgcattattt atctccattc ctttcctcta attaaaaagg tctggccaaa
    11641 tctccgcaga gtttgggcca aacctcagcc actgaaaatt cgttttaatt gtaaaatttc
    11701 ttcgttcatc cgtcgatgat cttcacgttt gcaatatctg gtgatttctt ggagcaaatt
    11761 catggccgca taccaatgaa atttttcaag catttcgtca gtcgggcgta tgccataact
    11821 gatcagccac tgattccagc tagacagtgg cacataattt cctaaaatcg ttgcaatatc
    11881 caccgcaggg tctgcaaaca tgattgaatc ccaatcgact agatataaat aatttttaca
    11941 tactaaccaa ttacgatggt ttacatctcc gtgtactact gataagtttt ccttatctat
    12001 tgacgggatc tgctcacgaa gaaaacgatg gacaatttgc agaaattgat ttttctggag
    12061 ttcttccggt aattcttttt catatttttg cagcatcttc tccggcgtca tgacacgacc
    12121 accgatacgg gtcagcatgg ttttaagaga atttgaatga tgtaaatggt acaaaacatc
    12181 aatcacgtcg tttctttgac caatctcatt aggagtcagc aagcgacctt ccagccactc
    12241 ttgtgctgtc aaagtatcgc cattccccgt tcgttttgtc caaacaagtt ttggtgtaat
    12301 tccttccctt gaaagagcag ccaacatcgg agtcgtgttt cgtttaataa agactttgtc
    12361 ctcatcacgc atgcctatgt atgtttttcc tgtgtcgcct ttgatgggac gtaaccgcca
    12421 ctcttggtct aactgaaact ccatcatgca ttctcccgtc ctcttactct ttgttttgca
    12481 gtacttatta ttctagtcgc ctgaacacta cacgtcaagc acatatacgt tctttttcat
    12541 ttaattgcca ttcaaaaaac cagcttttct caaaaaaaat agaacgaaca gcattcttac
    12601 tgtcaacgct ctatttttca ttctcatttt taccctacac agctttgttt tcagtctgac
    12661 atcttcttca aacgagttgg tacatatagt ttaacaaaga gcatagtaac aatcaaataa
    12721 gccgctagcg gaacccatcg ctgctgccca tctaatacga ctgcagcaat caaaccaaaa
    12781 ataaccgacg cgataattaa tacccatgtg agtagatact gaatcgcctg cttcttctga
    12841 ttcttatgga ttggataaag ctgaacgaga accatatagc ggaactggtt cgctaaaggt
    12901 aacagttgga aaccgatcaa atataaaaat aagaaaccga taatcagact aaaataaaag
    12961 tcgactactc cggcaatcaa taacccacct ataattacta atcggaaata aagaccacta
    13021 aaatcagaac ctcttgccaa tcttcttgca aacaagtaaa gataagtgtt ttttgattct
    13081 cttttaatat gagtcaatat aggatccaaa tagcgtcttc ttttgatctt tgccgtaatt
    13141 tcaggaacat ctgtgaataa attgataaat tgatagatct gatgcaacct atgttcttct
    13201 acatgcacca ttttctccca atccaatcct ttttcttgtt gcttccacaa aaaccagtaa
    13261 tatagacttg cctgtactaa tgcacctgct attcctgccc atattagcca ataaaatgat
    13321 agaaacaaaa agatcagaga agaaaaaaac cagcctaatt tccaataacg aataacttgt
    13381 tgatctagtc gaaacaattc tgctctcttg atttgcagat gactgaattt caaactccat
    13441 aatatgatca gatagacgaa aaacatcgaa aaggattgct ccgtactaac caccactaat
    13501 ggcatggcca atccgcatgc caatatcaaa aagatcaacg gaaaaatgca agaatagcga
    13561 aaagctcgtt ccaaataggt ttgcatttct ttttctttag gaagtaaaaa aacaatatct
    13621 gctgcctctg caaaagtagc catcttaccc acgaataaac agagcatcca aaatacagag
    13681 attatgagcc ctccaattgc aaacggtggc tgcagggttt tcaaccaatt agaataatat
    13741 aatcccacac cgccaatcaa gaaaagacaa actaaaacaa aatgatcgtt caatacataa
    13801 cgcatatatt tcatcatttt cttttgatgt cttgctaaac gtttttgaaa aaaactactc
    13861 atgtgcctgc tcctttgtta aagcaatata gatttcgtcc aaagaagaat ccggcagctg
    13921 aaattctttt ctcaacatct caagagaacc gtctgctcgt atctctccgt tatgtaagac
    13981 cacaaaacga tcacaatatt tttctgcagt agctaaaata tgcgtagaca tcaaaatacc
    14041 ggctccttct tttttcattt ctaccatcaa ttcaagcaat gcattgatag ctagtggatc
    14101 caagcctaaa aatggttcgt cgatgatata taaagaaggc tcgatcaaaa aggcgcataa
    14161 gatcatcact ttctgtttca ttccctttga aaaattagct gggaaccagt caagacgatt
    14221 agataaccga aaagtctcca acaacttttc tgctcttttc attgctatat ccataggtat
    14281 atcgtaggcc atcgcagtga cttcgatatg ttctctcaat gtcaattctt catataaaga
    14341 aggagtctct ggaatatacc caatttgttt gcgatattct tccgtatttt tctttaagga
    14401 tagatgattg atttctatcg ttccttttga agcttccagc agaccgataa tgtgcttgat
    14461 ggtcgtactt ttccctgctc cgttcagtcc aatcaaccct actatctctc caggctgtac
    14521 atcgaaactg atatctttta acacagggag atgtccgtac ccccctgtga catgatcgat
    14581 aattaaactc attctttttc ctccgccatt tatttttatt tattatacca cgaagaaaat
    14641 gacggtattt tgtttttttc cttacaatag ctttaactta tggtaaagtt aagctgaagt
    14701 atttattctt tatagaaagg atgttttata tggaaaactg tattttttgc aaaatcatca
    14761 atcaagaaat cccaagctat aaaatatatg aagacgacaa ggtctatgct tttttagaca
    14821 tctctcaagc aacgaaagga cacacattag ttgtgcctaa acgtcatgta gcagatattt
    14881 ttgaatatga tcccgaactt gcaggagagg ttttttcgcg tgtgcctaag attgctcaag
    14941 cattggaaaa agcttttcca gaaatgaaag gtcttaatat cttgaataac aaccgtgaac
    15001 tagcctatca atctgttttt cattcacata tccatttagt ccctcgctac agcaaagagg
    15061 atgatttttc tattcacttt gtcaatcatc aagatagtta tggatcggaa gaactaaaag
    15121 ctattcaaga aacaatcgta aagcaggtgt cctgcgatga ttaaaaactt tttcaaagga
    15181 ctgttatttg gagctgctgc tggcactgtc ggaggtctgt tattggcgcc caggagcggg
    15241 aatgaaacaa gaaagaaact gatcgaagaa ttggaagaag caacaactct gacaaatgat
    15301 ttaaataaca gcctgaatca ttttaaaagc gcattgatcg aaacaaaaga aacagctgaa
    15361 tctgtgattc ctgctttcca agaagcaatc aaaaaagata tcgaagattt taaattccaa
    15421 gccgaaccac gtatcacaca aatcaatgaa caagtagaaa aactaaaagc tgatctgcca
    15481 gagtatcctg aacaaatata ataatgatta ttttctcaac agatttaact atataagtga
    15541 gaaaagtttg cgtatttcct aggaatgtga tgaaattttg aattttgttt gaaactacca
    15601 cgacaacgtt tttaaaggaa atgggatatg gtatagttaa tatataaaaa taataactaa
    15661 ttacaggagt gcaaatttta atgaaaaaaa aatcaattat tttggcagct actagtgcgt
    15721 tggctgtttt aactctagca gcttgttcag gcgatactaa taaagatatc gcaacgatga
    15781 aaggcggaac gatcactgta tctgatttct atgatgaagc aaaacttgaa tcatcgaacc
    15841 aatccttagt acaacggatg atcatttata aagtattcaa taacaaatat ggcgataaag
    15901 taaccgataa gcaagtggat gcagaatacg acaaacaagc gaaatcatta ggtgatactt
    15961 ttgaatcaca attagaagct gctggatata cgaaagatac ttataaagaa tatattcgta
    16021 acaatttagc ttttgaagct ggcttgaaag ctcacgtaga tattacagat gacgatttga
    16081 aaactgcttg gaaatctttc catccagaag tagaagcaca aatcatcaaa ttatcaagtg
    16141 aagatgaagc aaaagatgtg aaaaaatcag cggatgatgg agatgatttc tctaaattag
    16201 ccaaagacaa atcaactgat acagagacaa aagaagatgg cggtaaagtt aaattcgact
    16261 caacgacaac aactattcct gcagaagtaa aagaagcagc tttcaaattg aaagacggtg
    16321 agatctccga tgttattact acaacaaatc ctacttctta tgcgacagaa tactacgttg
    16381 tcaaaatggt gaaaaaccaa aataaaggca atgacatgga taaatacaaa gatcaactaa
    16441 aagatatcgc tactgaaact aaactaagtg acaatgcatt cacaactaaa gtaatcggtg
    16501 aagaattaaa agatgcaaat gtaaaaatca aagatgatgc ctttgaaaat gtgttgagtg
    16561 cattcacaac aacttcatca agtacgaaag actcaagtga aactacagct tcaacaaaga
    16621 gttctgacac aaaatcaact gattcaacaa aagaatcttc aactgaagaa acaactgatt
    16681 catcaaaata aa
//
LOCUS       DADVXU010000060        16494 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.063, whole genome shotgun sequence.
ACCESSION   DADVXU010000060 DADVXU010000000
VERSION     DADVXU010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.063"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(51..1598)
                     /locus_tag="KB953_001831"
     CDS             complement(51..1598)
                     /locus_tag="KB953_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HBB6866804.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="KB953_001832"
     CDS             complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="KB953_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBB6866805.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(2043..2237)
                     /locus_tag="KB953_001833"
     CDS             complement(2043..2237)
                     /locus_tag="KB953_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866806.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
     gene            complement(2463..3665)
                     /locus_tag="KB953_001834"
     CDS             complement(2463..3665)
                     /locus_tag="KB953_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBB6866807.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(3668..3862)
                     /locus_tag="KB953_001835"
     CDS             complement(3668..3862)
                     /locus_tag="KB953_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HBB6866808.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(3909..4757)
                     /locus_tag="KB953_001836"
     CDS             complement(3909..4757)
                     /locus_tag="KB953_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HBB6866809.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(4962..5312)
                     /locus_tag="KB953_001837"
     CDS             complement(4962..5312)
                     /locus_tag="KB953_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866810.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            5907..6353
                     /locus_tag="KB953_001838"
     CDS             5907..6353
                     /locus_tag="KB953_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HBB6866811.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            6977..7969
                     /locus_tag="KB953_001839"
     CDS             6977..7969
                     /locus_tag="KB953_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBB6866812.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            7985..8869
                     /locus_tag="KB953_001840"
     CDS             7985..8869
                     /locus_tag="KB953_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBB6866813.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            8918..10525
                     /locus_tag="KB953_001841"
     CDS             8918..10525
                     /locus_tag="KB953_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBB6866814.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            10526..12010
                     /locus_tag="KB953_001842"
     CDS             10526..12010
                     /locus_tag="KB953_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HBB6866815.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            12187..13179
                     /locus_tag="KB953_001843"
     CDS             12187..13179
                     /locus_tag="KB953_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBB6866816.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            13231..14100
                     /locus_tag="KB953_001844"
     CDS             13231..14100
                     /locus_tag="KB953_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBB6866817.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(14520..16085)
                     /gene="guaA"
                     /locus_tag="KB953_001845"
     CDS             complement(14520..16085)
                     /gene="guaA"
                     /locus_tag="KB953_001845"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBB6866818.1"
                     /translation="MSSPTRFDEEPKIIVLDYGSQYNQLITRRIREFGVFSELMSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGK
                     VKPAKNREYGKAELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCP
                     IASIQNAERKMYGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGK
                     FGLNIIKVDAKERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(16140..>16494)
                     /locus_tag="KB953_001846"
     CDS             complement(16140..>16494)
                     /locus_tag="KB953_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBB6866819.1"
                     /translation="SQTEIVDELLSYNEELRTAYHFCQLLRYYFVKRGTEGFQETLKT
                     ISSTLPQSFKKKFTIFHRYQSGISNAFKVSHSNGVTEGLNNKIKLIKRIAFGYRNFYN
                     FRARIYLQQGLIFEN"
BASE COUNT         4989 a         2886 c         3341 g         5278 t
ORIGIN      
        1 tacgtcaaat aacccgacct atagaataga atcgggaatg tttggcgttt ttagttacga
       61 gaaaaatgtt gttgtacatc cttgttccaa ggcaacagtt gatctaaagt ttctgctgaa
      121 agtacgggaa gatttggtat cttatcaaat agaaactcta gatactttac aggattcaat
      181 ccgttaagtt ctgctgtttt atatacactc agaatgattc ctgaagttcg tgctccttca
      241 agacttgtgg aatgcaacca atttttccgt cctatcacca gctctttgat atttcgctcc
      301 gcacgattat ttgtgagttc taaacgacca tcttcaaaaa taggttcaaa accacttcgt
      361 agtttacacg catattgaac ggctgcatcc aacttggatt ttgcaacagg atctatcgtt
      421 gccatccact cataaaaagc aataaataat ggacgcagtt cttgttgccg acagcttaat
      481 cgcttctcag gagaaaagtc tttccatttc ttttctagga caaaccattt atccaattgt
      541 ttgactgctt gagctgcggg aatatccgtg tttttctttc cttttggaat tgcttcgaaa
      601 aatttccgcc gaacatgcgc gaaacaatag acaagtcgta cactttccac ggcattatac
      661 ccgctatagc cgtcacagtg taaataccct gtaaactcct tgagaaaagt tttcggaaca
      721 tctccagcac gactttcgtc gtggtgataa taaacgatgg gatgctgact gtcttttcct
      781 gtacaaaact gccagtaata ggtatctgtt ttggcacttt caatcacacg ataacttgtt
      841 tcatccccgt gtagaatctc ttcttgatta agatagactc ttaatcgttc tcctaaaggg
      901 tctaacgcgt tttgcactgc tttaatatgc caattggtga tattatctct cgaaatatcc
      961 aacccatgtg tttgtttcca atagttctct tgccgatagg caggaacttt ctgaccaaat
     1021 ttcatccgga tcgtttccgc cactaaacta ttggaaccta agctattaga aatcaaaggt
     1081 ttaggaacga tcgctttttt aaaagaagaa agtccttctt tttcgcagct tttacaacaa
     1141 taggtatgtt gataatgggt gagacaatcc aacatagcct gatggaaacg aatttcttct
     1201 cgtacttttg tccgtccgat atctttcatc tgtgaaccgc aatgttcaca aaattgttct
     1261 tccaaaggaa ggagatgttc tttctctgtt ataggtaagt gtttgatttt gtctgctttc
     1321 gttcctacaa ctcgttgatg tccttttact ggaataatgg tggcagctgc ttctggaaca
     1381 cttacagaaa tttcttcatc actaaaaaga ttcaattgcc cattcgtttc ctcaaagaga
     1441 gtctctttag agcgtccaaa taatttgttg gttaaataat tgaccttttc attcaatagt
     1501 tggatttcag ttgtaagatt ctgaatggtt tgttgttgtg cttgattggt tttatgggct
     1561 tcttgaagca actgaagcaa cgtatctgta gtttccaaaa tttttcacca ccttgaatcg
     1621 aactgatttt gttacagaat accacaagaa acgcacaaga aataggggtt caacacattt
     1681 tttgtgttaa ccccccagct taaaaaactc ctttttttcc tggcaaaata gctcgaggtt
     1741 gatcgatact taatccttct aacaaccatc gaacctgttg gttcgttaag gttcgaactt
     1801 cgtctttcgt ccgaggccat tttaacttgc cgttttctat ccgcttataa cagagaagaa
     1861 aaccatcacc gtcccaatag agaagtttga aacggtcttg acggttcccg caaaataaaa
     1921 aaacggcatc ttcgtaaagc tcgagttgat actcttgttg aataagactg gcaagaccat
     1981 caattccttt tcgcaaatct gtttttccgc acacaatata aatatgtttg gtttgaagta
     2041 aatcataaag cattggacaa cagttctttc aaaatagctt gaagaatata attgttaata
     2101 ccgttataga tcaaaacttc agaaccattc tctcttttga tgcgcatcgc taacttggca
     2161 gccgcgggtt tacctttttc tggtgcactc ttttcgtttc ctaattggac aggaacaatc
     2221 tcatttaatt tatacataga aaacctccag acttcttgat agctcaagta tatcttgagg
     2281 tgcatttgtt agtaaggtac tgttctatgg ggcgcttacg ttgaaaaacg ttgatttatc
     2341 ggcattcccc atccatatta tatttacgtc ttattcccac tcaatatgag tagcctcgta
     2401 aagaagctat tatatcagtg ctttttcagc ctattcctca ttttggtagg gaaatggtag
     2461 ggttagattt ttactgcttg atttccatta aagtttgcaa ctttagattt tgttgcacag
     2521 gtaaatttaa tgtttcctgt catttgttct gcaattcctt catcattacg gctccataga
     2581 tgagcgtagt gctttaaagt gatttcaggg ctagaatggc ctaaccgttg cgacacgacc
     2641 agaatgtctg cgttaaactc attgattagg tagctaacat gagaatgacg taatcctttt
     2701 ccttgtatta ggggtacctt agctagtttg gcataacgct gaataattcg ttgaacagtt
     2761 gaacgataga gtgggaggtc agtgtagcta agaataaatt gttctacacc atgatttttt
     2821 tgaataactt tccacttttt tagcacttca attgtatcat cgtccagaga aatgatccgc
     2881 aagccgcttt cagtttttgt gtaaggtttc cgaataaaat ctttttgatt tgtcatttcc
     2941 aaggtgtgat gaaccctcaa ctttttacgt ttgaagtcca cgtcattcca attaagtgct
     3001 aatccttcgc ttacacgaac accagtcatg taatatagcc agatcattaa aaaacacata
     3061 tgttcgtaga aatcatcttt ataaattttt gccaaaactt cttcaaattc ttcttttgtc
     3121 cagtaaggaa cgattgattt tccttttgga attgcttttg ttcttttgga aatattctca
     3181 ttcagaaatt gcagggtgac cgcatagtct aatgatttac gaaacatacc gtacataagt
     3241 gaactgtatg cttgtgagta tccgctatcg ttcaataacc aaattcgata attttcacaa
     3301 tctcgcacat tgatttcacg aagcttctta tcaccgaaaa attcagtaat ctgaatcaat
     3361 ccacactcac gagaactcca agtacttttt tgtacgctag ctttgtagtg tggaacgtaa
     3421 gactgctcca taaattcttt gtaagttaaa tcataattcg caaatccatt tacaaccatg
     3481 tattcatttt ttatacgcat gacttctttg tgtgcttcac gagctgatga gaatcgtttt
     3541 cctagagaat cttttgttcc tttcttctgg atcctctttc cagtcacttg atctgttcct
     3601 aactcaacta gataagaaaa gcgaccattt ttatcttgat acacacaagg gtattttgtt
     3661 ttcatagtta ttcctcctgt gaaagaggaa cacctataat ttcttcaatt acttgtcggg
     3721 gtactacccc aactctttta ttattataat atggatatcc ctgatttacc attcttgttt
     3781 ttgcttgtcg gattattgtg gtagcttgat gtgttttaaa tcctagagcc tctaaatctt
     3841 ttcgacaaat tgtatttttc attgtataaa ctcactttca aagaaaacac tatcattggg
     3901 taatagtgtt atttctttat ttttgtataa tgaacggtac tttcttgaaa atccatcact
     3961 tgttcaggac ttcccaactc atttaaagct aaagcattgt aaaaaaatgg acgtctaact
     4021 atatattcgc ttgtatatag tatatcttga ttcgtggaat catagggttt ctttaccatc
     4081 tttttggtga cagttggtag tttgagattc cgggacttga aaaaagcttt tttcttatgc
     4141 tctttcaatt ccagctcttt atcgaaatat ttgctgatat agcgcccgcg attttcgata
     4201 ctgtctacat cgatcttgtt gatcttaacg aaaccctgac cccaaatcgt agttaaccgg
     4261 ttaaatgaga taaaggggaa atcaaacaag ataatatggt agtggattgc accgcgttgc
     4321 tgcttttccc acgtggcgag atactttaaa tttgcttttt tagttttata gaggtcataa
     4381 ttcagtcgtt tgataaattt tttaaattca ttatttgcat actcaatgtc ttgaatgttt
     4441 tctttgaaag tgagagttaa aaatttcgtt tgattgtcaa aattcgtgtc tacaaggcgg
     4501 gcaatctcaa atcgcatact ttgataatgt ttctgtttgc gttttaaact gtcatattgt
     4561 ccttgagcgg ataattcatc atagtttttt cttttttcaa gagtaatatc attttcataa
     4621 atccaagcta atttttgttt tcgttccaga tttacttcgt tttcgatcgt tttacgtttg
     4681 ggagtaatga ttgcttcata atcataaatt tcaatatacg tgggagtttc aatgattttc
     4741 tgattataag cttgcaaaaa tcatcactcc ttgttgcgta ttcgattgtc aaagaaccag
     4801 tcattttctc ctgatgttga tctataaatc aagttaaggg ggaaggcctg cgtagcagcc
     4861 cttctagcct atggggtgtt gggcggtctt aacggacaga aacggctctg tgggcgctgc
     4921 cccgccgttt cgtcctaacc acccaacact ccatagtata gttagttttg tgtagtggta
     4981 ccttgttttc tgggaatcaa agttgccgta tcagcggttg ccgataaccc ataactgaca
     5041 aattgtccat tcgagcgtcc ccaaggtaca atcaccaaat tggtaaatgt aattatggct
     5101 aaaccttgtt gggtaagttc ttccggcgtg atcacaggat gttcagattt gactttgaca
     5161 ctgatttttt cgtagcgaag ttttggtagg atacattcgt agctccaacc tagaatttcg
     5221 ccctcctttt gccagcgagt gacattgacg acttggtatt ctttttcgag tgtttctttt
     5281 ggtaaactga gatcggtaat ttttattggc atcacaattc tcctttttta tacgaaagtt
     5341 tatacgattt gattaaaaaa tatagatggc catctacgac ttgtattata tacgagaact
     5401 tatacgtagt caagagattt tctaaaaaaa tatatacgat tttttatacg agtgttataa
     5461 tgaaaaaaat gataaggagg aatttgaata tgtttggttt agtacaacct aataaagtga
     5521 aggtgggcca acgaatcaaa gaaataaaag acggaatgtc tctctctttt acagaattag
     5581 ggaatcgatt aggtttaaaa aagccgacga tcagttccta cgttcaaggc tatgcgttgg
     5641 ctcctaagag tgtgatcaac caattatcta gtatcagcgg aaaacctgtc ggctggtttt
     5701 attttgggca tgtggaagaa tatatccgag agtatttgtt tctcattggt cagaaaaaaa
     5761 tagtggacga ttatcctgaa atcattgaga aaataaagca agaattttat acgggcgagt
     5821 tcaaaaatcc tgcttgggag aatgagctag gctatccttt ggaggaattt atccaagaat
     5881 gttttgtaga atattcgagt gaaatcatga aggactatgt cgtacagatt acgaaagagg
     5941 tacttgaaga atccaaaacg ctaaacaaac tatcatcaaa agcaaaagaa gaagctgtca
     6001 cgattctttc atctgggatc attgactata tcgagatgag tggtgagatc aagtatggag
     6061 agaaagatcg cattgcgaaa atggtgaaag aatcaataaa aacctttgat gtgaaacaag
     6121 acctgacatt taatgattcc tatttgattg gaaagttgat caatattctt ggtacagatg
     6181 aagatacgga gctgtttctt tcttctttat caatgatatt tactgataag catttttcca
     6241 ctcgatttgg tggggaagaa ttagtcgaaa tatttcaatc gatgcgacca gcgttaatca
     6301 aactctatcg ggagaaaaca ccagatgaat tttatgattg gtttgagaag taaaataaga
     6361 aaagtggctt agattacaag ttggtgaaaa aatttttttc agcataacat cagtaatcta
     6421 taaaggggtt gttgattata ctgtagaagt taagtactca agagattttt tgtacgtaaa
     6481 caaagcaaaa aagggtgcag tttcactaga aaatgtactc ttttttgtga tttttcttag
     6541 ttatagagtt tttttgaaac tttaaaaatg agaaaacata ttgattttaa agagtgtaga
     6601 catagcttct tttgctaaat agattaaact tactggtgag tggtcttgaa catactttta
     6661 aatcacattt tactgagttt tattggattt tataattcat tggaaacctc gtacttaatc
     6721 tgtttttatc tatcgactag aatctgtata tattccagct atcaatgagg ccgcagaaaa
     6781 gggtgcttaa aaataatctg ttgtttaaaa tattattatc agaatactta gatacaaaat
     6841 tctggcacaa agtaaaaaaa atattgactt aaaaaaatgg atatggtaat ctataaacga
     6901 aaatagtggt aacgtttcca aaatgcatct tcgttaaatt tttatttttt taaacgaaaa
     6961 tggaaacgtt tctaaaatgt attgggagga gattgaattg gaaaagacat tgggaccagg
     7021 tggtgagata agggcggtac aacgggataa aaaaaatatt tcgaagtggg aaaaattaaa
     7081 gaaacagttg cggcgtgata agtggatgta cttgttgctc ttaccaggta tcatcatgac
     7141 gtttatcttt aagtatgtgc caatgtacgg tgctgtgatt gcatttaaag actataatcc
     7201 tatgctagga attttggaaa gtccttgggt aggactagat cactttaggg aatttatcag
     7261 ttcaccaaat tttacgcttt tgctgagcaa cacattaaaa attagtattt atggtttatt
     7321 gttaggtttt tttccaccaa ttattttagc actgagcttg aatcaaatta tcaataaaaa
     7381 aataaaacag aaaatccagt tgattctata tgcgccgaat tttatctcag tagttattat
     7441 tgtggggatg ctgtttttgt tcctatccgc aaatggacct attaatgggt tagtagaaaa
     7501 ggttacgggg caaccaatta tgttcatgtc taatccagct tatttcagaa cgatttatat
     7561 tttatcggga atttggcaag gaatcggctg gtcatccatt ctttacaccg caacgctggc
     7621 gggagtcagt accgatttat atgaggcagc aaatatcgat ggggcaaata ttttacaaaa
     7681 gatttggcat atcgatttgc ctgcattgag gcctgtgatg gtgatcagct tcatcttggc
     7741 cgccggcggt attatgaatg taggatatga aaaagcctac ttgatgcaaa cttcaatgaa
     7801 catccctgcc tcagaaatca tttccactta tgtatacaaa gtcggcttgc aatcaggtga
     7861 ttatgcctac tctgctgctg ttggtctgtt caatatgatt attaatgtcg ttttattact
     7921 gattgtcaac aatataacca agcggctaaa cgatggtcaa ggcttgtaat gaaaggagaa
     7981 aattatgaat agtgctttat caaaaatcga ccgtcaaatc ttatggttga atcgagtgat
     8041 tgtaactttt ttagtcgtca tcaccattgt tccgctgatt tatgtactgg tagcttcttt
     8101 tatggaccca gctactttga tcaataaagg tataagcttc aatccgaaag attggacaat
     8161 acagggatat caacgggtat ttgcagatga ttccatttta cgcggttttg taaattcgtt
     8221 gttttactct tttagcttca gcttattgac tgtatttatt acgatgatca ctgcgtatcc
     8281 tttgtctcga cctaacttgg cggggaaaaa gattattatg actttttttg tcattacaat
     8341 gttcgtgggt ggtggcttga tacctaccta tttattggta aagaacttgg gcatgttaga
     8401 tagtgtatgg gcgattatcg taccaggtgc tataaatgtg tggaatatta tcttagcccg
     8461 tacctatttt agtgctttgc caaaagaact ttctgaagct gctactatcg atggcgccaa
     8521 tgacttgcaa atactgttca aagtcatgct tccattagcg aaaccaatta tgttcgtatt
     8581 gtttttatat gcctttgttg ggcaatggaa ctcatacttt gatgccatga tttacatcaa
     8641 agatcccgac ttacagccgt tgcaactcgt attaagaaat attttgatcc aaaaccaacc
     8701 ttcacaagac atggtgggag ccaatactgc catggcagaa atgaaacaaa ttgctgagtt
     8761 gatcaaatac gcaacgatcg tcatttctag tctgccatta atcataatgt atcctttctt
     8821 ccaaaaatat tttgataaag gtgttttagt tggttctgtt aaagggtaag tattgttcac
     8881 tatttgtatg aataaaaatt ataaaggaga ttttaagatg aaagcaaaat ggatgctgcc
     8941 aatcgttttt acttccgtac taattttggg ggcttgtgga ggctcaaaag aaaagagtaa
     9001 atcaagccct gattatgaac tagaaaatgt ctcttttcca ttgaaagaaa aagtatcttt
     9061 aaaaatgatg tctcaaagtg cccctttggc tcccaatgat cctaatgaca aattgatttt
     9121 ccaacgatta gaagaagaga ccaatctcca tattgattgg acaaactaca gtagtgattt
     9181 tgccgaaaaa agaaatttgg atatcgctag tggtgattta ccagatgcca tcttcaacgc
     9241 cgctgcaggt gactatgatt tattaaactg ggcggaaagt ggcgttattg taccgattga
     9301 agatttgatc gaagattata tgccaaatct gaagaaaatt tttgatgaaa accccgaata
     9361 ccgacaaatg tctactgcac ctgatggtca catttactct atgccttgga ttgaagaatt
     9421 aggcgaagga aaagagtcga ttcatacagt aaatgggatg gcttggatca acaaggagtg
     9481 gttggataat ttgggtcttg aaatgccgca aaccactgat cagttgatga ctgtgttgga
     9541 ggctttcaag acgcaagatc caaatggtaa tggtgaagca gatgaaattc cttttagctt
     9601 tattaacaat ggaggaaacg aagacttaaa atttttaatg ggggcattcg gtctaggtga
     9661 taatgatgac cacttagtcg ttgataacga tggtcaggta caatttactg ctaacaaaga
     9721 agggtataaa gaagccatca aatatttcaa tgaaatgaat aataaaggtc ttattgataa
     9781 agaggctttc gaacaagatt ggaatacgta tgttgccaaa gggaaagaac ataagtatgg
     9841 cgtgtacttt acttgggata aagcaaatat cacgggtgca gatgatagtt atgacgtctt
     9901 gccagtctta gcgggaccag atggaacgaa acatgttact cgtacgaatg gcatgggttt
     9961 ctcaagagat cgtttcgtaa ttactagtgc gaataaaaac ttggaactta cagctaggtg
    10021 gatagataaa atgtatgaac cgttacagtc tgtccaaaat aactggggaa cctacggtga
    10081 tgaagcacag gcgaatattt ttgaattaaa ggatggtatg ttaaaacatt tgcctttaga
    10141 ggggacagca ccgggggaac taagacaaaa aaccgaagct ggaggaccat tagcagttct
    10201 tgattcttat tacggcacag tcactactat gccagatgat gcgaagtggc gtttagattt
    10261 gatgcatgac acgtatttgc catatattac agaagacaat atctatccac gtgtcttttt
    10321 agaaaaagaa gaaacagatc gattagcaaa aatcgaaacg gatatgtggg attacatcta
    10381 tcgcaaaaga gcagagtgga tcaccggcgg taaaatcgat gaagaatggg aagaatacct
    10441 tgctgaattg gaacgtgtag gtttggaaga atggattcaa ataaaacaaa ctggctatga
    10501 taattttttg aagggagcac aataagtgtt agtagaagaa acggcgattt tggataaagc
    10561 aaatcgattt attgaaatga tgcgaggaaa agttaaccct cagtatcgac aaaactatca
    10621 tttttccgca cccatcgggt ggataaatga ccccaatggg tttatatatt acaaagggca
    10681 ttaccatctc ttctatcaat actatcctta cgatagtcga tgggggccca tgcattgggg
    10741 gcatgcgcgc agtagtgatt tggtccattg ggaagatcta ccagtagcgt tagccccaga
    10801 tcaaccttat gacagcggcg ggtgtttctc aggaagtgca attgaaagag acggaaaact
    10861 atatttattt tatacaggct gtcaagaaat tgatggtatt agccatcagc atcaatgttt
    10921 ggcggtctct gaagatggta ttcattttag caaatacagt ggtaatcctg tggtagcaga
    10981 aaaacagcta ggtgaaaatg ggctaataca agaatttcgt gatcctaaag taattgttca
    11041 caacgatggt tactatatgg tagtagctac taaatctaca cagtctactg gtagaatagt
    11101 gatgtttcaa tcgtcagatt tgtatacttg gcacttttac tctgtgctgc tagaagggac
    11161 ttcagagcaa ggaataatgt gggaatgtcc tgatttattc catttagacg gacgagatgt
    11221 cttaatcatg tctccgatac aaatcaagaa gaacggactg gaatatcata atatcagttc
    11281 taccatggct tgtattggtg aggtaaactg ggaaacaggt cagatgatag ttgataattt
    11341 ccatgaaatg gattttggaa tggattttta tgcaccacaa acaacaagag atcctaatgg
    11401 tggacgggtg atgattgctt ggatgcagat gtgggatcgt acattgccta cacatgagtt
    11461 aggccatctt tgggcaggct ctatgagttt gccccgagag ttaagagtga aaaaaaatcg
    11521 attggtacaa aaacccatct caactgtata tagtcattta gtttatcaac aaggcagtga
    11581 aaatattgca gtgactgaca catcagtcgt atttcgaaac gcgctgtctg attcttcata
    11641 tttacatcta gtcgcagatt tggagaaagc tgaagttttt acaattgaat tactgcggtc
    11701 taagacggat tgtctcaaac tagcctatga cactgctaca caggtttttg aattggatcg
    11761 tcagaaacta ggaagagagc ttacaggggc ggaagaagac cctctgaatg tacgaaaagt
    11821 tgaagtacct ttgattgata atcagctcgt tcttgagatt tttagagata cctcatcaat
    11881 tgaagtcttt attaatgggt tagaagttat gacggctact ttctatgagc tggaaaaagc
    11941 tcgagatatc ttgtttcatt cgatcggaga aagtcgtatt caagcttttg aaacagcgag
    12001 tgtgaaataa atgtttgatt gcttaggcga tgaatcaatt atttaatagg ttttgaagga
    12061 taatgttttt agaaaagaac agatagctag atttcccatg gatttgctat tagtattttg
    12121 aaaaaattag taatttatga caaaattcat tatactaaac ttgtgcagaa atgtgaggga
    12181 tcaactatga aagcaacaat gaaagatgtc gcaaaattag caggtgttgg agtaggaact
    12241 gtttcccggg tattgaataa cggttctgtt aaagcttcta ctagagaaaa agttgaagca
    12301 gcaatgacag agttaaacta tcaaccagat ttttatgccc gggggttaaa gatgaagcat
    12361 acgaacacgg ttgctttgat tctacccacg gtatggcatc cattttttgg cgaatttgcc
    12421 taccatgtag aaacagcatt agaaagacaa aactacaagc tttatttgtg taactccagt
    12481 ggtaatagtg aaaaagaaca agaatatatc cagatggtca gccaaaataa agtagacggc
    12541 atcattggaa ttacttattc agatatcgat gaatacattt cgagtaatct gccttttgtt
    12601 agtattgacc gttatttcac tgaagaggtg atttatgtga ccgctgacaa tgcggccggt
    12661 ggacgtttgg ctgcaaatga actttatcgc cgaggatgtc agaatgtagc atatatcggt
    12721 gggtaccaag aaacaccgaa tgaaacgaag agtcgtcgaa aatattttga aatcgagtgc
    12781 cgtcaactgg ggatttccta taaagcgtta gatatgttag aacctttaga ccatctctca
    12841 gaacgtgtaa caaatttttt gttagagaat cccgatatcg atgggatttt tacgatcaac
    12901 gatccaatgg cattagctgt cattgatatg cttgaaaaaa tagggaaaaa cgtaccagag
    12961 gatgtccaag ttatcggatt tgatggtcaa aagatgacag aaggagatcc ttatctagtc
    13021 tcaacgattg cccaacctac aaagcaaatg gcggaagtgg cagtggcaaa attaattgct
    13081 gtcattaatg gagagacagt agaaaaacgt accgtattgc cgattgaatt tggcgaaggt
    13141 gacacaacga aacctttagt gagaaacgat tttgcttagt gcaaatacgt ttcttttttt
    13201 gaaaatgagt gaatgaaagg tgtagataaa atgttatatg gtggaataga agccggtgga
    13261 acaaagtttg tctgtgggat tggtaatgaa caattacaag tgatagaacg gaaaagtttt
    13321 cctaccacaa caccagaaga aacgatggag caagtgtttg atttctttga taagtacaaa
    13381 ggaaatatcg catcaattgg tgtaggagca tttgggccta ttgatattca aatcgaatca
    13441 agaacttacg gatatatcac caacacaccg aagttagctt ggcaaaatta tcattttgta
    13501 ggagctttgg aaaacgcctt gtcggttccc atctattgga ctaccgatgt aaatgctgct
    13561 tgttatggag aatatatggc agggtatgga aaaggtaaaa atagtgttct gtattacacc
    13621 gttggtacag gtgttggtgg tggtgctgta gtggatggga aaatagtcgc aggttttagt
    13681 catcctgaaa tgggacatat gctcttacaa cgtcataatg aagatcaatt tgaaggaatc
    13741 tgtccttttc atcatgattg tttggaaggg ttagcatcag gacctgcgat tgaaaaacgc
    13801 tttggaagaa aagcccagac tatctcattt gatcatcctt attggacgat tgaggcagat
    13861 tatcttgctc agtgtgtgta taacacaacg ttactatatg caccggaagt aataatttta
    13921 ggcgggggtg taatgaaaca gccgcaacta ctcgagttga ttcgccaatc gtttaaagaa
    13981 aaaatgggtt cctacgtgga tacgcctact ctcgaagaat atctactaac tccaaggctt
    14041 gctgacaatg cgggcttgat tggctgccta gctttagcaa taaagaaact caatcattag
    14101 aatagtattt attagaacta ttcataacat accggaagtt taaaagggta ttctgaaact
    14161 taaatagtct caattcattc ttctttcttg agaatctgag tagagacttt tattctgttt
    14221 caaattaata taattacgaa atgatattga gtacaagaaa gttcttttta ccagatcttt
    14281 taaatcgaaa aatctgatat atatatttat agtactaaaa atctgattag ataaaaagct
    14341 aattcaatag gaaaataaag gtagtgaata aaagcaaagc ccttgttagt agttaatcta
    14401 acaagggctt tggtagggaa aatggtagga aaccatctta ttttttacga aaatcaggaa
    14461 ctttagtaat tgaataatgt tgatttatag gtattcctca tccatattat gttggctctt
    14521 tattcccact ccactgttgc aggtggtttg ctggtaatat catacacgat acggttaacg
    14581 tgtgcgactt catttacgat acggacactg attttttgca agacttccca agggatacgg
    14641 gcaaagtcag cggtcatgcc gtcaatagaa gtgacagcac ggatacctac agtgtagtca
    14701 tacgtacggc catctcccat aaccccaaca gagcgaatgc ctggtaggac ggtgaagtat
    14761 tgccagatgt cgcgatctaa accggcattg gcgatttctt cgcgcaagat agcgtctgat
    14821 tcacggacga tttctaattt ttcttctgtc aattcaccaa ggacacgaat acctaagcct
    14881 ggtccaggga atggttggcg ccaaacgatg ctgtcaggca tgcctaattg ggtacctaat
    14941 tcacgtactt cgtctttgaa caatgtgttc aatggctcga tcagcttgaa ttgcatgtct
    15001 tcaggtaatc cacctacatt gtgatgtgat ttgatggttt gggcagtttc agttcctgat
    15061 tcgatcacat cggtataaag ggttccttgt gcaaggaaag aaacgccttc ttcaccggca
    15121 agtttggttg cttcatcatc gaagacatat acaaattcat ttccaatgat tttacgtttt
    15181 tcttctggat cagacacgcc agcaagtttt gataagaaac gttctttggc atcgactttt
    15241 ataatgttca agccaaattt tccgccaaga ctttccataa cttgttctgc ttcaccttta
    15301 cgcaacaata cgtgatcaac gaagatacat gttaattgat cgccgatcgc tttttgtaaa
    15361 agaacaccaa caacagagga atctactccg cctgaaaggc ctaataaaac ttttttgtca
    15421 ccgacttgtt cacgaatttt tgcgatctcc atgtcgatga agttatccat tgtccagtca
    15481 ccagtacatt ggcagacatc tagtgtaaaa tggcgcaaca agtcatttcc atattcggaa
    15541 tggcgaactt ctgcatggaa ttggattccg tacattttgc gttctgcatt ttggatcgat
    15601 gcgatggggc aatctttgct ggtagcaacg acatcgaaat cagtcggtgc ttgggtaact
    15661 aggtcaccat gactcatcca aacggtttgt ttttcaggag tgttttggaa aagggccgct
    15721 tctgcagaag tgatttccaa ttctgctttt ccgtattcac ggtttttggc tggtttaact
    15781 tttccgccta atttataagt aatcaactgc ataccgtaac agattcctaa aactgggatc
    15841 cccaaatcaa aaatcttttc atcaatacca aaggccttct catcataaac actgtttggt
    15901 ccgccagaaa ggatgattcc tttagggttc attgccttta cttcttctgc ggtaatgcgg
    15961 tggctcatca actctgagaa aacaccgaac tcacgaatac ggcgagtgat cagttggtta
    16021 tactgactac cgtaatcaag tacgataatt tttggctctt cgtcaaatcg tgttggtgaa
    16081 gacaaatttg aataaaactt agtccgatat gcggcgcgtt ctgtgcgccg catatttttt
    16141 tagttttcaa agatcagacc ttgttgtaaa tagatccgtg cccgaaagtt ataaaagttg
    16201 cgataaccaa acgcaattcg tttaatcagc ttgatcttgt tattcagccc ctcagtgacg
    16261 ccgtttgagt gggacacctt aaaggcattt gaaatacccg attggtagcg atggaagatc
    16321 gtgaatttct ttttaaacga ctgaggtaac gtagatgaaa ttgttttaag ggtttcttga
    16381 aagccttccg tacctcgttt aacgaaataa tagcgtaata attgacaaaa atgatacgca
    16441 gttcgcaatt cttcgttgta gcttaacagc tcatcaacga tttctgtttg acta
//
LOCUS       DADVXU010000061        16323 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.064, whole genome shotgun sequence.
ACCESSION   DADVXU010000061 DADVXU010000000
VERSION     DADVXU010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.064"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            102..1529
                     /locus_tag="KB953_001847"
     CDS             102..1529
                     /locus_tag="KB953_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6866820.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1621..1986)
                     /locus_tag="KB953_001848"
     CDS             complement(1621..1986)
                     /locus_tag="KB953_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HBB6866821.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1983..2348)
                     /locus_tag="KB953_001849"
     CDS             complement(1983..2348)
                     /locus_tag="KB953_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HBB6866822.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2540..2788)
                     /locus_tag="KB953_001850"
     CDS             complement(2540..2788)
                     /locus_tag="KB953_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866823.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2787..2915
                     /locus_tag="KB953_001851"
     CDS             2787..2915
                     /locus_tag="KB953_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HBB6866824.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2972..4666
                     /locus_tag="KB953_001852"
     CDS             2972..4666
                     /locus_tag="KB953_001852"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HBB6866825.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4670..4789
                     /locus_tag="KB953_001853"
                     /pseudo
     CDS             4670..4789
                     /locus_tag="KB953_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            5317..6450
                     /locus_tag="KB953_001854"
     CDS             5317..6450
                     /locus_tag="KB953_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HBB6866826.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6481..6801
                     /locus_tag="KB953_001855"
     CDS             6481..6801
                     /locus_tag="KB953_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HBB6866827.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6830..7294)
                     /locus_tag="KB953_001856"
     CDS             complement(6830..7294)
                     /locus_tag="KB953_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB6866828.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7426..8679)
                     /locus_tag="KB953_001857"
     CDS             complement(7426..8679)
                     /locus_tag="KB953_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBB6866829.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8708..9025)
                     /locus_tag="KB953_001858"
     CDS             complement(8708..9025)
                     /locus_tag="KB953_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBB6866830.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9051..9371)
                     /locus_tag="KB953_001859"
     CDS             complement(9051..9371)
                     /locus_tag="KB953_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBB6866831.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9835..11250)
                     /locus_tag="KB953_001860"
     CDS             complement(9835..11250)
                     /locus_tag="KB953_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBB6866832.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11267..12583)
                     /locus_tag="KB953_001861"
     CDS             complement(11267..12583)
                     /locus_tag="KB953_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBB6866833.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12733..13431)
                     /locus_tag="KB953_001862"
     CDS             complement(12733..13431)
                     /locus_tag="KB953_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBB6866834.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13720..14676)
                     /locus_tag="KB953_001863"
     CDS             complement(13720..14676)
                     /locus_tag="KB953_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBB6866835.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14789..15622)
                     /gene="msrA"
                     /locus_tag="KB953_001864"
     CDS             complement(14789..15622)
                     /gene="msrA"
                     /locus_tag="KB953_001864"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBB6866836.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15797..>16323)
                     /locus_tag="KB953_001865"
     CDS             complement(15797..>16323)
                     /locus_tag="KB953_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866837.1"
                     /translation="FYLFMTVLLLITLTVSSKKNFNQKIISQPGHIVVNKETVEAYTT
                     KGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNLPYKTTGSSIIYRVTNEKSQVIT
                     LPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGVADIKVSTSYTVTTIISYLVTLT
                     AYLCLFFKIVFHKR"
BASE COUNT         5093 a         3283 c         2768 g         5179 t
ORIGIN      
        1 catttatatg ctttttcaga aattaactga tgatttcaaa aagaaaacat gtcataatat
       61 ttcccatatt gtgcaaaaaa atacaaggcg gaggctctac tatgattgaa gaatatattg
      121 agaaaaatat tttaagacag ctgtttttat gtgggcaatt ctatgtaaat aaagaagtca
      181 atctagaaaa gctctccaat ctcctgcatg tttgcaaaac cactttactc aatgatatca
      241 ataacataaa aaaagaattt gaagaacaga tcgcttatac acatcgggaa aaggaccgtt
      301 acactttata tttttcagaa catatacctc gatgcaagat catgcagcaa ttgtctcaaa
      361 attcgttgtt tttaaaaact tgtctgcttt atttggaaga agatgaacct gattatcttc
      421 aattaactga atgtgaattc atctctgtca gcaaagctta ttcattaaaa aaacaagtat
      481 tagcttattt taatgactgc gggatcgaaa tcgaccgcta ctcccctcga ttcactgaaa
      541 tggaaagacg tctgctgctt cttaatgtgt cgtatcgttt aggcggtttt aattcttggg
      601 agctacctga atcgtttttt gaacgtgccg atcgtttcat tgaatccgta acggaaaata
      661 gtggcagatt ttatgataaa gaaaataaag aaatcttatc gattggtttt gctatcagtt
      721 tcctacgcca gcaagtgtgt gctgtgacaa tagattcaaa atttatcgaa gaaatcaaaa
      781 aacgtcctat ttacaattat gtcgaatcag catgggaaaa cactgatttc caaacctatt
      841 ataaaaaaga agaattcgcc tttattttga cactattcaa tctttgtaat tatggtttcc
      901 attcttatca gttgattgcg gaagactttc aacagctaca ccaagtgttt attgacaata
      961 ctcccgaaat aaaagaacta gttgctacct ttgaatctca cttcaatcaa gaattattcg
     1021 gaaaccaacc gttcgaacgt gcgctgattc atctgatgcg ttcggcttgg gataactatc
     1081 aattattcat gccagaaaaa ttttatcttt taaatgagga acagacaaat ttatataaag
     1141 aagtccagac aattttttcc agctggtcca gccagttacc ttatgatcta cggttgaatc
     1201 ctaattgcat gcgggctttt gtgatcgaat tgagcggaat tctccgtctg acaaaagaac
     1261 atctgactat ctatatcgtc acaaattcgg atgttcatta tctgatttat cgggaagcct
     1321 tggaagctgt aactactttc gattttcaag ttgcaccaac aatttattca agtatatctg
     1381 atataaagaa gtatgcgcag cagtcctcaa accgggtatt atgtgaacgt acattatata
     1441 caccagatgc ggtccaatat gaaaatatta ttcctatttc gatcaatacg atcgatcgag
     1501 ctatcatttc agctgtgcaa aacaaataag gggctgtgac aaaaatcatg tcacagcctc
     1561 ttatttgttg aagatgccca tttctgtaac aatctcttga tacacgtcag gctcacaacc
     1621 tcacttattt tggacatcta cgggtccgat ctctttattt aaaaaataag aaatgatggt
     1681 tcggataact accaacgccg ctagttggaa aatatcctgc aaggtaggtt tgataatcga
     1741 ttcgatgatc ccagctgaaa caagaatttc caaacttaga agtatatagc ctcccaacat
     1801 ctttttaata gcattgttct cgctgactcg gtcgctttgc acttttgtat ggacaaataa
     1861 atatttcaaa aaatccttga ttgatagaca aacaccccaa accaataaac agatagcaaa
     1921 gatatcaagt cccagtacaa gtttttcgaa atatggaaga agaaactgca tcaactctgt
     1981 cgtcatccta cctctcctct tctttgtttt tctttgtctt ctatttcctg attcaagaaa
     2041 taagaaatga ctgttcgaat gataataata agtgcaagct tcaaaatatc tgtccaagtc
     2101 ggcttgataa ctgactcgat gatatcagca acgactagta cttccaagct taacaaaata
     2161 taacttgcta gcagttttct tgtatctgca ttacgagtat tacgttctgc atagtttttc
     2221 ttgacatgag accaatcaaa aagtttgaat agaacaagaa cgatcccgac aattaagata
     2281 ccgatagaac aaatatttaa aatagcaatg atccattcta gaaattcaat taaatttgag
     2341 cgaaacatat acgctaccta ctttctttta atttgttcct attcttttat tgtaaaagaa
     2401 agttaccttg ttcgtctatt taaaagttgt tctaatgata acggttcgct taaatttttc
     2461 tttttcactc tcccattttt ctccctccat ccataaatcg tggaagtatc caaaacaacg
     2521 tagcaagtta aaataaggct tagtccgtca tcagcttttc aaagaagcac tgtctcggtg
     2581 ctttttttgt acccaaattt ctaaaaatag aaccatacac ctcctcaaat cttttcttct
     2641 tacctaccct atttttattt acaggaaaat cagacacttc ttcttttttg tcaaaatttc
     2701 ctttttcttc tcgtcctatc atttggcatt ctaataaaaa cgaactatcc ttggatatag
     2761 aaagagaaaa caaggaggcg tttttcatga gtaaaaaatt aatcaaagtc ggcatcggtt
     2821 taggtttgct tgcccttggc gctgcatatc taggaaaaaa gacaggacta ttcgaagatg
     2881 acagtcattt atatgatgaa ttcgaatcaa tctagcatcc tatttagttg aatcactacg
     2941 gacatacttg gaagacattg gaggaatcac aatgagaaaa agtaaagcaa taatgattgg
     3001 tgccggtatt gccaatatgg cagcggctgt ttatttgatt caagaaggca aatggaaagg
     3061 ggatcagatc accttttatt cattggatga tcacggttca aatgatggtg caccgaccga
     3121 cactgtagtg gatgaatatt ggaataaaaa ccatccatta gaaaaccaaa aaggctatat
     3181 tgctcgaggc ggtcgcatgc ttaattaccg tacctatgta gatttgatgg atctgttaag
     3241 tcgaatacct tctgttactg agccagggat gactgctgaa gaagatacga gagattttga
     3301 tgcaaaacac cgaacctttg acaaagcacg tttgatggaa ggtggcaaag gaatcattga
     3361 tggcggaaaa ctgggattca ataataaaga ccgtattctt ctgactaaac tgattgctat
     3421 gcctgattca gaagaagaaa aactagacaa tatcactatc gaagaatatt tcaaagatga
     3481 tccacacttt tttgaaacaa acttctggtt tatgtgggaa acaacatttg ctttcagaac
     3541 acgtagttct gctcaagaat tgcgtcgcta tatgcatcaa atgatttatg aattcactca
     3601 aatcgaacat ctagtgggcg tcaatcgaac acgttacaat caatacgaaa gtattatgct
     3661 tcctttgatc gagtacttga aagaacaagg atgcaaaatc attttgaacc gccgtgtcac
     3721 tgactgggaa ttcaaagaaa caccgatgca agatgaaatc actgtgactg gtttgaaaat
     3781 taccaataca ctgactcaag aagaagaata cgttgtggtc gatgaagata cagcagttat
     3841 tttcactaat gggtccatta cagattcagc tactttagga gattacgaaa caccagctgt
     3901 agaaaatatg gattatggtg cagcttctag tctctggaaa aaagccagtg aacgtttcta
     3961 caacttggga aatcctgaca aattctttgc tgatcgagat gcaagcgaat gggtcagctt
     4021 taccctgacg acgaaaaacc atttgttgtt gaatgaaatc acccgtatca cgactcaaga
     4081 accaggaaat gccttgaatt catttatttc aaccacaccg atcacgccgt taggccaaaa
     4141 agatgtcaat atgtctattg tcgtgcatca tcaaccgcat tttacgtctc agaaaccaaa
     4201 tgaaacagtt atttggggtt atttccttta tccaagacga cgtggcgaat ttgtggataa
     4261 acagtatatt aaaatgaatg gaaaagaaat gctagaagaa ctgattggac aactttcaaa
     4321 agtagatcct ggaccagtga atattcgtga aaaagaaaca gaaattttcg acagcgtaat
     4381 aaataatatc cctgtctata tgccttatgc ttccgcgttg ttcaataatc gtgccaaaag
     4441 cgatcgacca aaagtcattc ctaaacattc gaccaatctg gcctttacag gagaatttgt
     4501 cgagcagcct tatcagatga tctttactga acaaagtgct gtacgttctg gagaaatcgc
     4561 tgctttccat tttgcgggta ttcctatgtc ccgtcttgtc aaaacaccgc gttacgacaa
     4621 agatattcct actctagctc gtgccgcaaa aaaaatgttt gaataatcaa gaggttgtga
     4681 aaaagctgcc ctgcatcaag taataagaac aaccaggcta aaacagcttc ttatgttttt
     4741 gatttggttg gacttattac cgcagatgca agcttttgaa caccgtttac caagaggtta
     4801 tgaaaaagct gccctgcatc aagtaataag agcagccaag ctaaaacagc ttcttatgtt
     4861 tttgacttgg ttagacttat taccgcagat gcaagctttt gaacaccgtt taccaagagg
     4921 ttatgaaaaa ggcgtttagt ttcgagtatg aagaaaaatt ttgtgaaaat ggttcctcac
     4981 gtttttgcat aaatttgact taataccgag aaactgatcc cttctgtcat aacctctttt
     5041 tatttatgtc tgtttctttt tgaaaacaac tattttttca acgtttactc tcttgccaat
     5101 ccaacatcat ctaacggata caactatttc aatgattacc attacactaa aaaatcggaa
     5161 acacacctct ccgaacacaa atacataaga ctatagaatg tttcatttaa gaaaaaaagc
     5221 gtttcattat caaaaaggta agatctcctt taaaatgaaa ttatctgtag tactaataat
     5281 gcagacaatt ttatgaatct gaaaggagca tctccaatga aagcagtcac ttggcaagga
     5341 aaacagaaaa tggaaatcag aaacgtagat gatccaaaaa tattagaacc aacagatgca
     5401 atcatccgta tcacagcaac agccatttgc ggctccgatc tgcatcttta ccatcatggt
     5461 gaagcagttt tggaaaaagg gtatgttgta ggacacgaac caatgggaat cgttgaagaa
     5521 gtgggatctc aagttcaaac attgaaaaaa gggcaacgtg tggttatccc ttttaatatt
     5581 agttgtggtc attgtcattt ctgcctgaat cacatggaaa gccaatgtga tgaatcaaat
     5641 ccagatcagt tatatggcgg gcttttcgga tttggcaagt tgaacggtaa tcattggggt
     5701 ggtcaagcgg aatatctaag agttccttat gcagactcta cttcatttgt tgtaccagat
     5761 aatgatcttc ctgatgaaaa agttttattt ctatcagata ttttgcccac agcttattgg
     5821 agcgttgaaa acgcaggagt aaaaaaaggc gatacagttg tgattctagg ttctggtcca
     5881 gtcggtctat tcgctcaaaa attcgctgtt atggctggag cagagcgcgt aatcgcagta
     5941 gatccagtac agcaccgtct agataaagcg gctaattaca atggggttga aacttatttc
     6001 ttagaagaca cggcacaagc tggaacagaa ttgtatgaac tggctaaagg tggcgcagat
     6061 gtcattatcg attgtgtagg aatggacgga ttagagcctg tcaaagaaaa agcgaaaaat
     6121 ttagtaagct tacaaagcgg cacaatctct ccattacaga tggcatcgca agctgtcaaa
     6181 aaatttggaa cagtccaagt aactggtgtt tatatgactc cagcttcttc ttacccgctg
     6241 caagaatttt ttatgcggga tatcgctgtc aagcatggac aggcaccaat tatccatcta
     6301 atgccaaaaa tttacgcaat gattgcagaa ggactattcg atcctacaca aatcattact
     6361 cattcgctgc cactatcaga agcagcacaa gcatatgaga tctttgacaa aaaagaagat
     6421 aaaaatataa aagttgtttt gaaaccttga attcagtaaa aaatacaggt gattttcttt
     6481 atgcccaaaa tcaaacgaaa tacccatgag atcgaaatag gaaaagaaga agatatcgaa
     6541 atacgaggac gtcgcttccg cttctatttc caaaatcgtt acacattgat ctcactggct
     6601 gtcgatttat taaccgggat tttctatatt tttggaagcc ttgcttcctt aactaaaatc
     6661 cccgatcgat atggcaccta tttttatttg gctggtgcta ttttcttgac gattcgtcct
     6721 attctcagaa tcgtaaggaa catttttatt tacgacgaaa aaaaagcaat gaagagaaca
     6781 agcaaaaaaa aagaggcatg agaatttttc aaatctcatg cctctttttt caccctatcg
     6841 tatcactttg atacagacga gcggtcgtcc cactttcttc cttgaccatt gtcaaaaatg
     6901 tccactcgta tcgttcataa aacgtggtat gatctgtaat taagtaaagt ttttcgtacc
     6961 caagttgatg catttctttt ctgatttccg ctaatatccg cttggctatc ccctgcttac
     7021 gcttttccgg ttcaacaaac agggcacaaa gatttggtgt cagatcaggg cggttatgga
     7081 agtcgttttc aatgacgccc gctcctccga cgatggtttc tttttcatct aagaccacat
     7141 accattgagg aatactttcc ttattagcga ttccagtacg aatactttcc tcatacacaa
     7201 cagcaggaac tccccatttt gatgaaaacc aattcgcgct ttgttttact aattctgggt
     7261 gttctcttaa tttgatccag ttatatgtaa ccaatgttta aacctccgac taaaataaaa
     7321 cgtttacaaa aattatacaa gtctttatga aaattagcaa tgacaacaat agtgatgtaa
     7381 aaaaaaaaac ggaaaacaat cacttgttct ccgttttttg attaatcaag catctaaagc
     7441 ttcttttctt ctttcttgga tcgtagctac cttgacgaac ggtagataga tcaaaataga
     7501 aagaaccaaa ttgacagcgg ctagtacacc acctgcaata ctttttgttg ccaatacacc
     7561 gccaataatt ggtggagtta cccatggagg cataacggta gctgctggca ctaaacctgt
     7621 agaagtcgca aagtaagcaa cactaactaa gaccattggt gtcaaaataa atggaatgaa
     7681 catgatcggg ttcaacacga taggtaaccc aaacatcgtt ggttcattaa tattgaagac
     7741 acctggcgca atcgacaagt ttgttacaac catatatggt ttgtttttac gtcctactaa
     7801 ataaatagct aacagtaaac ctaaagttgc acctgttcct cctaagttga caaagctgtc
     7861 aaagaaaggt ttgttcacga taaacggtgc gctatgtcca gctgcgattg cattaacgtt
     7921 tgcttcaatg gctggcgcat tgattgtttg catcaaagga tcgaccatat tcgctccgtg
     7981 taaaccaaag aaccataaga atggtgtgat aaatgcaagc agtaaggcag atggataaga
     8041 gtttgcaagt cccataaatg gttcttggac cactgtatag aaagaaccta cgatatcatc
     8101 aacaccgaat gcagcaaaaa ttgcagcaac taaagcaaac gcagagatag tgatcattgc
     8161 tggtagtaat gaagcaaatg cttttgcaac tgccggtggc acaccatcag gcattttgat
     8221 gatcaatttt tcatttccgc ttaaacgtac aaagatttct gttgaaataa tcccaacaat
     8281 aatagcaata aacaaaccgt ttgagctcat acctaatgct ccgcctaaag cgaagaaaga
     8341 gcctaaagaa atcacacctg cggaaacacc gtcttttcgg tagccgttag ctaaatgata
     8401 agcaagcaag aaagcaatca agacagaaag aatggcaaat gtaccgttcc aagcagcagc
     8461 cccgaatcct ttccatgatt caccaccaaa aattgagttc atcaattctt ggaacccggg
     8521 aatcggtaag ttattgatca ttactgctaa tgctcctaag atcattaacg gcatactcac
     8581 cataaatgag tcccgaatcg caactaaatg ccgttgtgca ccaatttttg atgctacagg
     8641 tataaaatgt ttctccataa atgcaacaaa tttatccata aatcttccct cttttcttta
     8701 ctgtagatta cgcgttcaat cgacgataca gatcaatgat ctcttttgct aaatctgtaa
     8761 aagctataga tgtcatcaga tgatcttgac tgtggaccgt caaaagcgtg acttcaacgt
     8821 gatttccttg tgcttcttgt gtcagcatct ctgtttgaga gtgatgtgct tggactaaag
     8881 attcctctgc atctttgatt ttttcatctg ctaaagtgaa atcacctttt ttcgcagcag
     8941 caatcgcttc cattgcatcg ctttttgcat ttccgccaaa catgatcaga cccatgatgg
     9001 cttctagatt tttttgatct tccatttatt gtatcctccg ataattttta ttatttgttt
     9061 attagttttt ctgcttggtc gagtactttt tcaccattca tcatgccata gtcagccata
     9121 ttgattacat ctaaaggaat gcctttaggt gctagctttt cagcaaattg attttgcata
     9181 aagcgtactt gcggtcctaa aagtaaaacg tctacatttt tagattctag ttgattatcc
     9241 gcatctgaag cagatacagc aaagatgtct gcttccattc cttttgcttc tgctgccttt
     9301 tgcatttttg tgactaggag acttgtactc attccagctg aacatacaag catgattgtt
     9361 ttcttttcca ttttaaacac gttcctttct atacttacac ttgtatataa gcaagtagcg
     9421 tgccaacttt aaaagcgctt taaaaagttg atatcatcac atttaaggtc tataccaagt
     9481 tgcatacaca ctaaaaaaga aagcgcacac taaacacaca aattaatttt tattaccttt
     9541 tttcttttaa cgtgcacaaa aaaagttgtg acacaaaact tgtcacaact tttttcaaat
     9601 aaacggtatt caaaagcttg catctgcggt aataagtcca accaagttaa aaacataaga
     9661 tgttgttttt actttgctat tcttattact tgatgcagga cagctttttc acaacctctc
     9721 aataaacggt gttcaatcag ctgaccctgg gtattagctc aaaacgctgg attcacaacc
     9781 tcttttattt caaataacca gtaaatacaa tgaacatcta caaaaaactt ctatttattt
     9841 atagatttcg tcaggcaaat ccaattctct cttatcaatc aaagtctgat accacgaagc
     9901 agattttttc aatgttctgc ttcgttcatc gttcaattcg attcgaacta atccatatcg
     9961 attcttaaat gcattcattg gtgacacaca gtcagtgaat gcccataaca tatagccttc
    10021 gcaatttgcg ccttcttctg ttgctttgat caagtaatgc aaatgctgtc cgataaattc
    10081 gatacggtaa tcatcttgca cgattccttt ctcatccata tactgttctt catattctct
    10141 tcccataccg ttttctgtta tcagccaagg gatattatga taattttctt ttagatacat
    10201 ggctatatca tacatgattt tcggatagat ttcccatccc ctggaattat tcattttctt
    10261 cccaggtaaa tcaaagactt cataatatcg ttcaggatgg aaaggtgtct cttcattcca
    10321 ttgataacga ggggcgcgaa cgcgtttagg ataatattga ttgattccaa ggaaatctac
    10381 tgtatgatgc tgaatcgttt ctaaatcctc tgttgcagga tcgaaataaa tcgattgttt
    10441 ttgacacaac tccaggaact cttctgtgta gtatcctttt accatcggat caaaaaatac
    10501 tcgattataa aacaaatcat acatataagc agctttttgg tcttctggag aattggatcg
    10561 agcatagacc atttcaggat ttaaaataca gccaatcctt ccaccataat ttccgctgcg
    10621 gtaaatctct actgctttag ccgttgctaa aactttatga taattccaca tcatccactt
    10681 ttttgtgttt tgttcatgcg gccagcgaac agcatccaaa tagcaacgcg tttggggaac
    10741 gattggttcg ttgaatgtaa accagttttt taccctatca ccgtaacgat caaaggcaat
    10801 ttgagcatac ttagcataaa aatcaactac ttttcttgaa gaccagccat catatttttc
    10861 caaaagaaaa gctgggactt cataatgttc caaacaaatc atcggttcta cgtctgcttc
    10921 gatcaaagag tcgatcattt cacttatatg ctgagcatat tcttcatcta ctatacagtt
    10981 ctcataatca gtaaaaaaac gagcccaatt gatagaagtc cgataatgtg tcagatggat
    11041 ggatttcatc agatccacat cctgctggta ttgttccata aaattagttg ctacggctgg
    11101 gccataccca ttatgccaaa caaaatgttc atttttatac cagctatcta taaacgaatc
    11161 ctgtccttct ttttttcctt tccaaccttc cgtttgccaa gcagaagatg cagcaccaag
    11221 aataaaattt tttggaatag taattttcat gatttcctcc tgttttttag atcccatcat
    11281 tttccacacg atttgctact tttacaaatg gcagatagat caaaatagaa acaataatac
    11341 aaacaagttg cgtaataact gcaccgatat ttcctgctgt tgccagataa gcattgacga
    11401 tcggcggcat tggccaaggg accattacaa ctgctttcgc cgcaaatcct aaagcggttg
    11461 caaaataacc aatcgttcca gtgataagag gcgttaagat aaacggaata gctaaaattg
    11521 gattcagcat gatcggcata ccaaaaataa ctggttcatt gatgttgaag atcccaggag
    11581 caagggataa cttggtgatt tgtttcatat cttcccgttt tccaactaac aatactgcaa
    11641 tcagtaaacc gatcgtgact ccagacccgc ccatactcat atacatatcc caaaatggca
    11701 tagtaataat gttcggtact tcttttccag cttcaaaagc ttccgtattt acagcaattg
    11761 ccgctaaaag caacggttcc cgtatcggct tcaccatttg atttccatgg ataccaatca
    11821 cccaaaagac ttgcgcaata aacatcaata gcaaaatacc cggtaaccct tgtacgacac
    11881 cttctaatgg gcgttgtacg acattgtaaa tgatatcata aaggtacatt cctgtaagtt
    11941 ctttgatgat aaatcccgca gccgcgatac ccgatacagt cagaatcgtc gggaataaag
    12001 cagaaaaact agcagaaaca ttcgttggca cggtatcagg cattctcagc tgtagtttct
    12061 tctgtttcct caaccagcaa taaagttcaa tagaaaggat ggagaccaac attcccaaaa
    12121 ataacccttt cgtatctgta tattgtttag ccaatacgtt ttctactata acagtctgat
    12181 tttcattcac taattcataa acagttggat taacagataa ataagacatc actgcgatga
    12241 taccagggaa tacaccatcc actttattca gcttggctaa ttcgatccct atcagaaaaa
    12301 cagcatagat ggtcaagaaa cttagtgtga catagctgat ggcagatgcg ataggcttta
    12361 attcttctaa aaaacgcaag aaacttatct gtgctaagcc attttccgag tcaaagacca
    12421 ttgctgaaaa caatgtgcca aaagcacccg taataattac cggaatcaat gcagcaaatg
    12481 cgttttttat caccatgata taccttaaat tattgattct cgttgccacg ttccctaaaa
    12541 cattggctaa tttatccata aaactcattc cagatcccct cattcctgct tcttctcctt
    12601 gacgtcatct ctttttgtct attagtcagc gcttacattt cgatgaacag aaactattta
    12661 ctcctctttt ttaccaaata taaaacaaaa attcatctga tgtatttatc ataaaataca
    12721 tcagatgaac tgtcaattag tttctttttc tcctattatt ttctgtaatt ctctccgatt
    12781 tattgccata tggacagtca ttgcatcaaa cgcagcaagt gcattccgcc tttcgatggc
    12841 ctgaacgatc tctgtatgaa tatcgatcgt ctcgattttc agatgctgga tatcataatt
    12901 tttattaaac aaagcaattg attcattaat gattgggata atatgcagca aggctacgtt
    12961 tccgctagct tctgcaatca tcgtatgaaa atcgatgtct aattgaacat gttcttgtgt
    13021 gccttctcga aaagaagttt caatcttttg cgttaatttt ttcatttgga cgatttgatt
    13081 cttcgttgca tattccgctg ccaaacctgc catcttaggc tccaataaat atctcagttc
    13141 aaataagtct cgtatcatcc gatcctgatc ttcaatcaaa gaaaaaccga gtgggtcatt
    13201 ggcaatccct cgcttatcac tgacaaatgt ccccgctcct tgccgaactt caaggatatt
    13261 cctggaagct agagcccgca cagcttctcg caccgtgctt cgccccacct ctaaataatc
    13321 ggacaattca tattcattcg gcagtttatc tcctacttgc aactgcattt cctttaaata
    13381 tctaaggatc ttgtcagtag ttacttctac tagggtttta ttttcttcca aaccacatac
    13441 ctccttttca tctgatgtat tttttcgtaa ttattataac acgtttgaat catctgatga
    13501 ttttttgtat acaaaattga ttaatccgtt catagacata caaacgaaga agatatttac
    13561 cataaaaaag actaggataa aattcactcc tagccttcga tcatttttat tttgaacgac
    13621 aaaaaatatg atgttcaata acagacgatc ttcattcgat tcttgatgtc atcttttgtt
    13681 ttttttaata tatggataaa aagtttgatt acatcgtttc tattctagaa tttggtcgat
    13741 tctcaattct tcttctggtg agaaatctaa attatccatc attcgaacac tttcttgtaa
    13801 ctgacttaac cgacttgctc cgaccagtac acttgtaaca gatttttgtc gcaaattcca
    13861 tgcaagggcc atttgcgcta atgattgtcc tcgtgatacg gcgattgttt gcagtgcttt
    13921 gattttttcc agagttgatc caacttgttc ttctgaaagg aatggtattt ccttacgatg
    13981 tgctctagaa tcttcaggaa taccatgtag ataacgattt gtcagcaacc cttgagcaag
    14041 cggactgaac gtgattgccc ctagcccttc ttcttctaat actggagata gatcgtcttc
    14101 tagtagtgca cgagaaaaca tattgtagcg catctgatga ataatgaacg gtgcttcctt
    14161 tctttttaaa atttcagtca ttttttttgt atcttctcca ttatagttgg atataccaat
    14221 atataatgct tttccttgtc ttacaagttg catcaaagct tctgctgttt cttccaaagg
    14281 tgtatcaggg tctgggcgat gatgatagaa gatgtcaaca taatctagcc ccattctttg
    14341 gagactttgg tcacaacttg aaatgatgga tttacgagag ccccattccc catatggccc
    14401 ttcccacata tgataacctg ctttgctaga aataatcagt tcatcacggt aggcttgaaa
    14461 atcttctcta aaaatacgcc caaagttttt ctctgcagct ccagcagggc ctccataatt
    14521 attcgcaagg tcgaaatggg taattcccat gtcgaatgcg cctcttaaaa tctctctttg
    14581 attatggata ggatcataat caccaaaatt ttgccataat ccgagagata aaatcggtaa
    14641 tttcaaacct gaatgaccgg atttacgata ttccattttt tcataacgtg cactatctgc
    14701 aatatacaat ttttctcctc ctcatcaaat ctttttaaaa cgctttcttt tttctatttt
    14761 tagtttacta cattttaaag aaaaggagtt aatcaaaatg attttttcgt atattttttt
    14821 gccatttgtt ttttactcgt tgatagatca gaaattgatt acgtgcttgc tggatttttt
    14881 tataacgttt cggttgtttt ttgtaaaatt gctgatgata gttttctgct ggccaaaacg
    14941 taccggctgg ttcgattttt gtgacgattg gttggtggaa agttcctgaa ctgtctagtt
    15001 tctgttttga ttgttccgct aattcttttt gtcgttcgac ttgataaaaa atcactggac
    15061 gatattgctt ccctctatct tgaaattgtc caaatgtatc tgtgggatca gtaatttgcc
    15121 aatagattgt cagcaactcg ctataactta tttctcttgt gtcataaatg atctccacag
    15181 cttccacatg acctgtgtaa ccaccactta cttgatcata atttggtttt tcaacatgtc
    15241 cgcccgtgta tcctgacaaa acagatagga tccctttttt cgattcaaac ggctcgacca
    15301 tacaccagaa acatccccct gcaaatactg cacgttcttg atagtcagca tcttgcatgg
    15361 catgcttgga aaagtcatat ttttgctcct tatcaaatcc ttccgtaatc aataaataaa
    15421 agtccattac atcaggcgtc agattcattc ggatagctaa tggacgtagt gttgcttcta
    15481 gttttgataa ctgttcttta acggatatat tttctcttat gtcattcttt gtctgaatca
    15541 atacttttcg ttcccaatct cgtgtattcg gattcaaaat cagattataa agtgtttgaa
    15601 tgaatatttg ttcctcatcc atagattctc ctcctttccg aagaagacaa gctagtgact
    15661 ttagctgtaa tttatcataa agtgaggaaa taagcgatta cttcatcccc ctacagattt
    15721 ttcgtataaa aaagagagaa tccttatcga cagattctcc tttttaatca atttatttta
    15781 taaatgaaaa gttaatttac cttttatgga aaactatttt aaaaaataaa cataaatatg
    15841 cagttaatgt aacaagataa gaaataatag tcgttactgt ataacttgtg cttactttaa
    15901 tatcagcaac tcctttgact ccgcttattt gtaccaatcc attagcattc acctgctcct
    15961 ttacaggttg atgatttatt tctacattcg ttcctaaata agcatataca ggaagtgtga
    16021 ttacttggct cttctcatta gttactctat atatgataga actaccagtt gttttataag
    16081 gaaggtttac ccattgactg ccaatctttg cttcgtgttt tattatatgc tccttattct
    16141 ccaatgaagc gataggagaa taatcatata aaaaataatc cattccttta gtggtatacg
    16201 cctctactgt ttctttgttt acaacaatat gtcccggctg agaaattatt ttttgattga
    16261 aatttttttt actagaaaca gttaaagtga ttaataacaa aacagtcata aataaataaa
    16321 ata
//
LOCUS       DADVXU010000062        16304 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.065, whole genome shotgun sequence.
ACCESSION   DADVXU010000062 DADVXU010000000
VERSION     DADVXU010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.065"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..632)
                     /locus_tag="KB953_001866"
     CDS             complement(<1..632)
                     /locus_tag="KB953_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866838.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDL"
     gene            complement(811..1530)
                     /locus_tag="KB953_001867"
     CDS             complement(811..1530)
                     /locus_tag="KB953_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBB6866839.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(1661..2812)
                     /locus_tag="KB953_001868"
     CDS             complement(1661..2812)
                     /locus_tag="KB953_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HBB6866840.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(3029..3769)
                     /locus_tag="KB953_001869"
     CDS             complement(3029..3769)
                     /locus_tag="KB953_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBB6866841.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(3773..4096)
                     /gene="rsfS"
                     /locus_tag="KB953_001870"
     CDS             complement(3773..4096)
                     /gene="rsfS"
                     /locus_tag="KB953_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBB6866842.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(4153..4746)
                     /gene="yqeK"
                     /locus_tag="KB953_001871"
     CDS             complement(4153..4746)
                     /gene="yqeK"
                     /locus_tag="KB953_001871"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HBB6866843.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(4730..5380)
                     /locus_tag="KB953_001872"
     CDS             complement(4730..5380)
                     /locus_tag="KB953_001872"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBB6866844.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(5390..5701)
                     /gene="yhbY"
                     /locus_tag="KB953_001873"
     CDS             complement(5390..5701)
                     /gene="yhbY"
                     /locus_tag="KB953_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HBB6866845.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(5717..6826)
                     /gene="yqeH"
                     /locus_tag="KB953_001874"
     CDS             complement(5717..6826)
                     /gene="yqeH"
                     /locus_tag="KB953_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HBB6866846.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(6823..7350)
                     /locus_tag="KB953_001875"
     CDS             complement(6823..7350)
                     /locus_tag="KB953_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HBB6866847.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(7530..8315)
                     /locus_tag="KB953_001876"
     CDS             complement(7530..8315)
                     /locus_tag="KB953_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HBB6866848.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(8308..9177)
                     /locus_tag="KB953_001877"
     CDS             complement(8308..9177)
                     /locus_tag="KB953_001877"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HBB6866849.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(9178..10557)
                     /locus_tag="KB953_001878"
     CDS             complement(9178..10557)
                     /locus_tag="KB953_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBB6866850.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(10565..10984)
                     /gene="fabZ"
                     /locus_tag="KB953_001879"
     CDS             complement(10565..10984)
                     /gene="fabZ"
                     /locus_tag="KB953_001879"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBB6866851.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(11009..11485)
                     /gene="accB"
                     /locus_tag="KB953_001880"
     CDS             complement(11009..11485)
                     /gene="accB"
                     /locus_tag="KB953_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBB6866852.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(11488..12723)
                     /gene="fabF"
                     /locus_tag="KB953_001881"
     CDS             complement(11488..12723)
                     /gene="fabF"
                     /locus_tag="KB953_001881"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBB6866853.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(12742..13479)
                     /gene="fabG"
                     /locus_tag="KB953_001882"
     CDS             complement(12742..13479)
                     /gene="fabG"
                     /locus_tag="KB953_001882"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HBB6866854.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(13491..14408)
                     /gene="fabD"
                     /locus_tag="KB953_001883"
     CDS             complement(13491..14408)
                     /gene="fabD"
                     /locus_tag="KB953_001883"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBB6866855.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(14515..14739)
                     /locus_tag="KB953_001884"
     CDS             complement(14515..14739)
                     /locus_tag="KB953_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBB6866856.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(14792..15754)
                     /locus_tag="KB953_001885"
     CDS             complement(14792..15754)
                     /locus_tag="KB953_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HBB6866857.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(15764..16204)
                     /locus_tag="KB953_001886"
     CDS             complement(15764..16204)
                     /locus_tag="KB953_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBB6866858.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         4296 a         3719 c         2789 g         5500 t
ORIGIN      
        1 tccaaatctg tttcaggata ttttaatttg acaagcgaaa taatcatttt cagttcttcc
       61 atgttttctg gatattctgg gtcaaatacc ttgctatgac ggccaatccc gccattgtcg
      121 atcaatcggt aaattcgatt ccattcaatg aattggtcaa ttgattggaa attgctgatt
      181 ggatagccgc cacaatccaa aatctctgat acacgaaatg ctaggtatgc actatcgatc
      241 actttatttt ttttcgtgtc tatcttctca tttctttcga taagaatttt ttcaggacga
      301 tgtttcaagc tccactctaa tagtagaaag aaagcctcca tccgatactc ttcttcaaat
      361 aaccgaaagg ctgaatcaga acgacgaaaa agcagcggaa tcagataatt ttgtctataa
      421 atcagcattg gtgcgcggtc caccatacac tctaaataac ggttcagata ccctgtaccg
      481 acatagtttc ctaatcgata gccttcaacg ccgaatcttg cgtatttttg cgctaatttt
      541 gaacaatcag ttacgttcac agctaactct tctactttgc ggtaaccgct taagatacgt
      601 tcaaaaggtt ctaaagtgat ttttgcttcc atattcttcc ccgtccatct tccacatact
      661 tcatatcatt tcacatgata cttatgctct catcatacca caggttgaat tagaaaagaa
      721 aataaatcac acaaaaaagc aagacaatct gttagtgaga ttgtcttgct tttcgaaaaa
      781 gactgaatac ttttcgttaa aactggttgc ttagtcttct aatgccacat tatggaacac
      841 atgctgtaca tcttctagat cttccagtgc atcaatcatt ttttcaaatg tttcttgatc
      901 ttctggagat agtgttacct cgttttgagg gatcatttgg atctcagcga ttgaaaaatc
      961 agtgattcct ttttctttta atgcttcttg tatactatgg aaatccgcta cttcaccgta
     1021 aacgatgatt tgtccttctt cttccattac atcgcgaaca tcgatgtctt tttccattag
     1081 gtattcaagg atctcgtcag cgtcttctcc tgcaaaagca aagattgctg tattgtcaaa
     1141 catgtaagaa accgctccgg aaacacccat gttcccgcca tttttcccaa atgctgcccg
     1201 tacgtctgct gctgttcgat tcacattgtt cgtcaatgta tcaacaatca ccattgagcc
     1261 gcttggtcca aatccttcat agcgcaattc agagtatgtt tcatctcctg caccttttgc
     1321 tttttcgatt gctcggtcaa taatatgttt tggtacatta taagttttag cgcgatcaat
     1381 gacgaaacgt agtttttggt ttgcatgcgg atcaggatca cctgattttg ctgccgcgta
     1441 gatttcaatc ccaaattttg cataaatccg gctattatta gcatctttag cagttttctt
     1501 tgctacgata tttgcccatt tacgtcccat attttcactt cgctttctga tttgatagtt
     1561 tcataacgca aaaacgtcct gttccattat acttgtgaat cacgattctg tgaagctttt
     1621 tttctttctt ccttcattct tctcattttt agtcaattat ctatactcgg attgggattt
     1681 gcccgaaatt ctgttccgga atattgcctc ccaattgata gatttgatca cttctaacta
     1741 acagttccgc ttcttgtgcc tctttttttc cgattcggga ataaatagaa atctcttttt
     1801 tttcttttaa gaatcttctt ccagcatcgg taaatccaag tagtcgaaga taatttttat
     1861 tttgttggac aggaatctct tgctttttga tattcagcaa gacataacaa gataatcgtt
     1921 ggatccgtgt ccatgtatat ctttttgttt ttacctttga cacaaaatcc tcaaaactat
     1981 cagcagttgc agctgctgtt ttcatgcgcg attctattcc ttcggtcatc tgatagatat
     2041 ctgtcagttc cagcaaagta gaagataaca accgataacg caaatacggc caataagatt
     2101 cccaagtctg cagcgaacaa gttgaaagat cttcttctgt ctgaggcggc acaagtccag
     2161 ctattctttt tccttcttct aacgcatggc gaatggctgt tgcactggct atcctctgat
     2221 ctgtgatgat tttttcatga tagcctgcac ttttacgtcg gatcggatgt aattccatag
     2281 ggtggacata cttggcattt tcctttgcat aacttaatcc caagatatga tttggcgaag
     2341 aaaaatcaag cgtaatatcg ggatacagac tgcgaaaaac ctcagtcatt ttttgcggat
     2401 aactatacgt ttgatcagac agttcttgaa atgcctcttc tatcctatct tgattctctt
     2461 ggacaaagcg tccgaaactg ctgtaatcaa acgaagtctc ttcttctgtt ccaaaacaca
     2521 aactgtcaca gtccagtgtt tgcaagattt ttactgcacc tttagcaaaa tagtcagctg
     2581 actgaaccgc ccattcgacg ggaagctcca ctactaaatc cacaccattt tgtaaagctt
     2641 cattcgcgcg atgccattta tcgatgatag ccggttcgcc tctttgcaaa aaattcccgc
     2701 tcattacagc aatgacgacc tctgctcctg ttatttttcg tgccatttcc acatgatagc
     2761 gatgtccatt gtgaaaagga ttgtactcta cgataatccc gcagcttttc atgcgcatcc
     2821 tccttcatag ttatcgataa aaaagaaata gagacaagta aaaaaaggca aaaatcggaa
     2881 aaaatacccg aactatataa actattctcg caaacacaat acttctgcga cgaaaagctg
     2941 cactgcaaga gcaatgccta tatcgttcgg atatataatt tatgttgaag atttattctt
     3001 ttcttgttaa ctcgagcagg caaaaccgct agtctttgta tgctacaaag aaccagcgct
     3061 ttgatttctc tgatgctgct tcgtctgtaa aatcagaaaa tgcttcgact ttcgaaaagc
     3121 cgctgttttc cagcattctt aaataacttt ccaatggata ggtacgctct tgatgtagtt
     3181 catcttcacg aataaatcga ccagaccctt cttgttcttc aacgaaaaag gtaagaaaat
     3241 gttcgatgct gtgtttctcg cttcctggat aactatccca caaaaaagca aaattatccc
     3301 cttgataatg atagctatat tctggaaaaa cctcatctac ttgataggtg gagtgtacgt
     3361 caaataaaaa tcggccattt tcttctaacg cttgatagac ttcatcaaac acttgctgta
     3421 cctcttgtct atttttcata tagcacagcg aatcagaaaa acaggtaatt gcctgatatt
     3481 gtccgatttc tgataaatcg agcatattgc cttccacaaa ttggatcgat gcgtcttctt
     3541 catatgctcg ctggctggca atcatcaaca tctcttctga aaggtctaac cctgtcactt
     3601 tatatccatc ttttgcaaag gcaaccgcta atgctccagt accgcatgcc agttccaaaa
     3661 tattttttgt gtcttcttgt aaatgacgtt tcgaaaaagc taaccattgc tgataaagtg
     3721 tttcgtccat cacttcatcg taaatgaaag caaatgtttc ataagccata tgttaatcca
     3781 accattcact aagatcgact agcggagcat ctgaccatag tttttccaga ttatagaaat
     3841 ttcgttctgg cgcatggaaa acatgtacga tcacatctcc aagatcgatc aatacccatt
     3901 ttccgccttc ttttccttca atacgtttta cttcatattt gttttcttct tctttatcaa
     3961 tgatttcttc tgtaattgcg ttgatttgac gttcgctatt tgctgaacag atcatgaaat
     4021 aatctgccaa caaggaaaca tttcgaacgt ctaaagcgat aatgtcttct gcccgttttg
     4081 aatctgctgc ttcaatcgca atttttaatt gttctttact atctatgatg atttcctcct
     4141 aatatgagca gcttattttt ttgctaccca gtaattatat gtttcgattg tttttggata
     4201 aatctgttgt tcttgcgcaa gcaaatgggc caatgtatgt tttgtttcaa atgctacagc
     4261 ttcgtctaga tcagcccatg caatggctcg tgcatcttgt accccaggaa aatctcgacc
     4321 tggttcaata tagtctgcta cataaatgat tttgtcaagg agactcatct tagcagcccc
     4381 agtcgtatgc acccggattg catgaagaat ctctgcatcg gtgattccta attcccgctc
     4441 tacaaacgaa gcgccaacaa gaccatgcca gattgcatta ttataattca ataactccgg
     4501 atcaaaaccg tctcttacga ttaccatttt gaactcctca tctggtcgtt cttttgcata
     4561 atcatgtgtc aatgcagcaa tactagcttt ttcaggagaa gcaccgtatt ttttggcaag
     4621 agccacggct gtttcttcta ctccaagcac atgcttgaag cgttgttcgc tcattctcat
     4681 ctgtattttt tgcatcaggt tttctctaga ataagcagta tattcattac tatagtccat
     4741 attcatacaa cccttcattt tgaatataat caattacttt atctggaaca aggtagcgga
     4801 tagaacagcc ttctttgatc ttttgacgta ttttcgtgga actgatgtcg atctcaggta
     4861 catctaccca aattacagga tatggagtat ccgttgtgta tcctggtcga cgtataccca
     4921 caaagttgac catagaagtc agctcatcga ttttatacca ttttggcaaa tattctacca
     4981 tatccccacc gataataaag taataatctg tatccggatt gttttgcgtc aattccttca
     5041 tagtatcata ggtgtaactt ttcccaccac gagctaattc aatcgtttca atctgcaaaa
     5101 aaggattgtc ttccaccgct aattccaaca tgttgagtcg atgttttgca tcaatcgtct
     5161 gtttctcatc aacatgaggt ggctgataag aaggcatcaa aaatacgcgg tcaagaccta
     5221 aattccggcc agcttgttca gccatcacta aatgtgcaag gtgaacggga ttgaatgttc
     5281 cgcccaagat ccctacttgc tttttcttct ctaagaattg aggcatttct tcaggaaata
     5341 attgtgaacg aacaaatgtc ttagcttgcg tattcatcat ggtcactcct tatattgctt
     5401 ttacttcttt tgatagcctt tgatactttt cttttgtgga aggtttgtat aatacaataa
     5461 cacgtccaat gatttggacg atttcgcaat ctatttcttt ttctaatact tgagctgctt
     5521 cttctgcaat ctcatctgta ttttgaagta acgatacttt gatcagttct cttttttcta
     5581 aagcttcttc aatttgaacg atcattgctg aattgatccc accttttccg atttgaaaaa
     5641 tcggctgaag atgatgcgct ttgctacgta aaaaacgctt ttgttttcct cttaaactca
     5701 taattttcct tcttccttaa atcaatgctt tgcgtttcaa tacatctaca ccctttggcg
     5761 cccagcccgc aattactact ggcgctgtta cagtgatcca gcctagaccg gcaaaaacga
     5821 tgtctgtttt ttctttcaca gaaaactcaa aacgaacaag ctccggaaat gcctcgactt
     5881 catcaggacg aggaggctgt aataatccac ctacatgttt ttgataaaat tcgtctgctt
     5941 tttccagttt cgttcgatga atatttacat catttgacac ataagcaata aatgaagcac
     6001 gattgccgct gataaaatca aatcttgcaa gtccgcccaa gaaaagtgtt tgcccttcat
     6061 tcagctgata aactttaggt ttgatttctt ttaacggtgc aatgatccgc aaatcttttt
     6121 tacctaagta gtgcgccatt tgatggcgat ggataattcc cggtgtatca atcaaaaaat
     6181 gcccgtcatc taaaggaatc tcgattttat ccaaggtcgt tcctggaaac tgcgaagttg
     6241 tgattacatc ttgtactccc gctgtatttt tgatgatttg attgataagc gtcgattttc
     6301 cgacatttgt tactccgacg atatagacat cccgtccatc gcggtattct tcgatttttt
     6361 ctaatagttc cgtcatctca tgagatttct tagcactagt taataataca tctactggac
     6421 gaattcctgc ttcgtaagca cgttccttca tccattgtgt taatttagtt tttttcaatg
     6481 atttaggtaa tatatccact ttgttcccaa ctaaaagtac tggattgtca ccgacaaaac
     6541 ggtgtagtcc tgggattagt gaaccattga agtcaaaaat atccaccaca ttgacgatca
     6601 atgcatcttt ttgccctaat tcattcagta agcgcaaaaa gtcgtcatcc gtcaatgaga
     6661 catcttgggt ctcattataa tggcgtaatc tgaaacaacg ctgacaatag acttccccgg
     6721 aagctaaacc tttttccaaa gccgtttgcg gcgtgtatcc taattcaccg ggtttgtttg
     6781 tttgtataac ggcaccacag ccgatacatt gtaattcttc tgtcattcga ttccacctcg
     6841 ccagcccata tcagggtgtt ttgctaatag ataggccata attctttttt cccaaaaacg
     6901 attaaattgt gtcttccaac tatctgttgc gataatgggt tttacaagga tgctacgtat
     6961 ccctgcacga ttggctccac gtatatcagt cataatctga tcgcctacca ttactatctc
     7021 atcatctgct aaatccaatt gtttctttgc tatgttgatc cctcttgcta aaggtttcat
     7081 tgctcgtgca acatacaaaa gatcaaattt ttcaatcgct cgcgcgacac gttttgaatt
     7141 attgtttgaa acaacaacaa cagggattcc tgcatttttc atttctaaaa tccaagtaag
     7201 caactcttct gttccatctg gattattcca tgcaatcaac gtattgtcta aatcggtcaa
     7261 tactgctttg atgcctagat tctttaattg ggcaggtgta atctgataga tggcttctac
     7321 catccatgtt ggtttgtaat ttgaaaacat gctgtctcct catctgcaaa aaatgaagga
     7381 gcacataaag tactcctcaa ccccgtatta tactgcatat cagcaaaaaa aactacctct
     7441 atactcgttc gtctttcaga aaaagcattt ctttacaaaa tgaccaaaaa taaagattga
     7501 tcacagaaaa atttagaaac aaaaaacaac taatattggc ggaacctctt gtatcgtttt
     7561 tccagtaatt tatgctgatc taatcgaaca agttccgtca atctttcaat caacgctttt
     7621 ttcataatgt gtgcgacttt atatcgtggc aactgctcgc cgttgaacgt ttcaggaata
     7681 actcgatcaa cgattcctaa ctcgcgtagt tctggcgcag taattttcat caactcagct
     7741 gcttctgcgg cgcgactacc gtctttccac aaaatcgacg caaatccttc tggagaaagc
     7801 acagcataaa tcgtttcttc cagcatccag acttcatccg ctaaacctag agcaagggca
     7861 ccgccacttc caccttcacc aatgatgatg gcgatgatcg gtacctttaa atcagacatt
     7921 tcgagcagat ttctggcaat agcctctcct tcgccgcgct cttctgctcc tacaccgcaa
     7981 tatgcacctg ctgtattgat caaggtaatc accggtctat tgaacttttc cgcttgtttc
     8041 atcagtcgta aagcttttcg atagccttca ggatggggag aaccaaaatt tctgcggata
     8101 ttttcctgca gatcccgtcc tttttgaata ccgatcactg ttaccggttt gccatctaat
     8161 cgagcgattc caccaacaat cgcttcgtca tctgcaaata aacgatcacc atggcattcc
     8221 tcgaaagaat cgaagatctc ttcaaaaaaa tccaagcttg tccatcgttc tttagctctt
     8281 gctaactgaa cgacatcatg tgctgtctta tccacgatct atccatcctt tctgtgtatg
     8341 caattcaaga agcgtatgga ttttctgttt caattccatg cggggaacaa tctgatcgac
     8401 aaaaccatga gataataaga attcagcctt ttggaaatct tctggaagtt cctgcttgat
     8461 cgtctgttcg atgacgcgtc ttccagcaaa accgatcaag ctttggggtt ctgctagaat
     8521 aatatcgcct tccattgcaa aactggcagt tactcctccg gtcgtcgggt ccgttaaaac
     8581 agttagataa aaaagacctt cattgctatg ccgtttgaca gcagcagaga tttttgccat
     8641 ctgcattaga gaaaagattc cttcttgcat ccgtgcgcca cctgaagctg tgaacaagac
     8701 gactggaaga ctttcctttg ttgctcgttc gaataatctg gtgatctttt ctccgacaat
     8761 cgttcccata cttcccatga taaaattagg atccatcact ccaataatga agggaatccc
     8821 ctgaatggtt gctttaccag tgaggactgc ttcatgaagt cctgttttat cttgcatttt
     8881 tttaattttt tccgtatatt cgggaaatgc tagcggatct ttggttacaa gatccgtatc
     8941 ccattcttca aaactttttt catcaataat caaagctaga cgctgccatg caccgattcg
     9001 aaaagcgtat ccgcaatgag ggcatacttt ttcttcaccg atgtctttcg tataaaggat
     9061 atgtttgcaa tttggacact ttgcccacat gttatcagga acagatggcg agttagcagg
     9121 tttgctctct ctattaggat tgatccgaat atatttcttt ttcttaaaca gagccatcta
     9181 tgcgcctcct atctccgttt ctggtgtcca attcggtaaa aattcttctt gtaaaaatgc
     9241 tgtgctgtaa tcacctgcta tgactgcagg atgactaatc aaatccatct gaaattctgc
     9301 gttcgtgatg attccttctg tgataaattc acttaaggct cgctgcatct tcattaatgc
     9361 ttcaaaacgc gtatttccat ggacgattat ttttgcaatc attgaatcat aatatggcgg
     9421 gatcgtatat cccgggtaaa ctgcactgtc tactcgcagt cccatcccac ctgctggaag
     9481 cagtaagttt tggatcttcc ctggagaagg agcaaaatgg aaagcaggat tttctgcatt
     9541 aatccggcat tcgattgcgt gtccttggaa tactacttct tcttgacaca cattcaaagg
     9601 ctcaccggct gctatctcca cctgtttttt tactaaatcg attcctgtga ccatttctgt
     9661 tactggatgt tccacttgga ttcgagtatt catttccatg aaataaaaat cgccttcttc
     9721 atccatcaga aactcgatcg ttccggcatt ttcataattg actgctttag ctgctcgaac
     9781 agcagcgtct cctagtgctt ctctttttgt ttgtgagatc actacagaag gagattcttc
     9841 cagtactttt tgattgttgc gttgtaaaga acaatctcgc tcgcctaaat gaatgacatg
     9901 accaaatcga tcacctaaga tctgaacttc tatatggcga gcaggataaa tgattttttc
     9961 caaatacata tcatcatttc caaatgccgc tgctgcttct tgctgagctg aagaaaaatg
    10021 ctgtgggagc tcttcttttg attgaacttt ccgaatgcct ttcccgccac cacctgcagc
    10081 tgctttcagc atcactggat aaccaattct gtctgctatt tccaatgctt cttctactgt
    10141 agataataca ccagtacttc ctggaatgac tgggacattt gcttcttgca tcaattgacg
    10201 cgcattgatc ttatttccca ttgcatcgat cgttgcactt ttaggaccaa taaatgtgat
    10261 attgcactct tcacacatgg aggcaaatcg gctattttca gataaaaaac caaatcctgg
    10321 atgaattgct tcggcttttg tcacgattgc agcgctcagt acttcctgaa cattcagata
    10381 tgaatcaacg gcttttgccg ggccaatgca gattgcttca tctgctaact gtgtatgaag
    10441 tgcttcggca tctgcttctg aatatatcgc tacggtttgg atccctagtt cgcggcatgc
    10501 acgaatgata cgtacagcga tttcaccgcg gttagctatc aatactttag aaaacatatt
    10561 tcacctatcc aatcataaag gttaattccg cttcggctac ttttttaccg tctactttag
    10621 cgattccttt gcctattccg gctgaagctt tcactttcaa tatctcaact tcaaggtata
    10681 aggtatctcc cggtgtaact ttcttacgga attttgcttt gtccaatccg ccgaaataag
    10741 ccgtctttcc cttgaattgt tcaagagaaa gtaatgcaac tgctcctgct tgagccattg
    10801 cttcaacaat caatacccct ggcataacag gttcttctgg aaaatgtcct tgaaaaaagg
    10861 gctcattgat cgttacattt ttcttagcaa cgatccgttc tccttcaacc atttcttcca
    10921 ctcgatcaat caaaagcatt ggataacgat gtggaatgat ttcttttatt tcttgtatgt
    10981 tcattgtttt ccctctttcc tgctttgctt atttttgctg aatcctaaag agtggctgtc
    11041 catattcgac aactgactcg ttttctacaa ggatttctgt gatcactccg tcggcctcac
    11101 tagttatctc attcatcagt ttcatcgctt caataatgca gataacttct cctttggcaa
    11161 cttgatcacc tacttttttg aaagctggtt tgtcgggcgc tggttgcaga taaacgattc
    11221 ccacgattgg cgaaacaacc tcgattccat caaatactgc cgattctttt tctttcatta
    11281 tttctggagc agttgttgct tgttcaggta tgacattgac tggctccgaa actggcaacg
    11341 tttcgtttgt ttgaaccata ttcatgcctc gacttgtttt atttttattc atatacaatt
    11401 caaattgacc ttcacgcaaa tcgaattccg ttagcgagga ttgatcaaat tgactgacta
    11461 attctctgat ttcattgata tccaaaatta ttcctcccag cgtttcatgc acagaaccgc
    11521 attatgtccg ccaaacccta atgaattact cattgcataa gtaaaacttg caggtttgct
    11581 ttcgtttttg acaactgtca agtcaatctc aggatcttgt ttttctacat tgatcgtagg
    11641 tgggataaat tgatgttcta atgccagcaa agttgccaag gcttcgatcc cgccggctgc
    11701 tcccaataaa tgtccagtca tcgatttcgt gctgctgacg taagtatctc tgaaattttc
    11761 acctaatgcg atttggattg cttttgcttc tgcctggtca ttggctggtg tacttgttcc
    11821 gtgagcattg atatatccga cttgttccgg ctgtacacca gcttcttcca tcgcaagctt
    11881 cattgcttta gatgcaccag aaccatctgg agtaggagct gtcatatggt aagcgtcaca
    11941 gtttgcaccg tatccaacga tttctccata gattttggca ccgcgagctt gtgcatgttc
    12001 taaagattca agaactagaa cgcctgctcc ttcacccatc acaaaaccat tccgattttc
    12061 gtcaaacgga atcgaaccac gattaggatc agttgattct gagagtgctg ttaaagctgc
    12121 aaatccagca atccccatct cacaaatcgt tgcttctgtt cctccagcaa gaatgacatc
    12181 agaatagcca tgtttgatgt tacggaatgc ttccccaatt gaattcgttc cgctagcaca
    12241 agctgttacg atagctgtac aaataccttt tgcacctaca cgcaaagcaa tatttccagc
    12301 tgccatgttg gcaatcgcca ttggtacgaa cattggagaa actcgttttg cacctttttc
    12361 atgtagacga ataacttggt tttcgattgt tggaagacca ccgatcccag atccgatgat
    12421 gacgccaaac cggtcttgat tcattttttc tttatctaaa ccgctcatct ccattgcttc
    12481 taaagcagcg tagattccat agatcgaaaa catatccatt cgtttgctat cttttttaat
    12541 aaaatattta tcgtaaggga aatctttgac ttcacccgct aaggtaatcc ctgtttctga
    12601 agcatcaaat ttagtgatcg gtgcgatgcc attcgtcccc tcttttaagc tattcaaaaa
    12661 gctgtctgcg tcatttccaa taggtgaagt gactccatag ccagtaatta ccactcgatt
    12721 catttgtttg ctctcctttt cttatccgtg catgaccaat ccgccatcga cattgatgac
    12781 ttgtccggtg atatatggat tttgtgctaa gaaaactgct gtttgtgcaa tatcttctac
    12841 ttgtccgaaa cgctgcatcg gaatttgctt ttccgcttgt tccttgactt tgtctgctaa
    12901 aacttccgtc atatcagttg tgatgaatcc tggcgcaatg gcattacatg taatgcctct
    12961 agttgcagct tctcgtgcca cagattttgt taatcctact acgccagctt tactagctgc
    13021 ataatttgct tgaccaatat ttccaatcaa cccagataca ctagagagat taatgatggc
    13081 cccttctcgc tgcttcatca tttttttcaa tacgtgctgc gtcatattga aggttccaat
    13141 caaattgata tccagacatt ttttgaaatc ttctgcatcc attcgcatca caagtttgtc
    13201 atttgtgatt ccggcattgt ttactaacac atggatcgat ccaagctttt cttctgcttc
    13261 tttgatcatt tgtcccgctt tctcataatc agagatgtca ccggaaactc ctacgcattt
    13321 tacaccgaat gcttcgattt cttctatctt ttcttttgga atctctccgc gaccattcaa
    13381 aatgatattt gctccagctt ttgcaaaagc taaggccatc gctttgccga ttcctcttgt
    13441 actacctgtg ataaatacat tttttccttt tacatccata acgttcctcc ttatagtatt
    13501 gctattgttt catcaaatgt ctgcttattc tcaacacgca gaactggtat tgttttatcg
    13561 atttttttca taaatccgct taatactttt ccggggccga cttcgatgac ttgttcgatg
    13621 ccgattgttt ttaacttatg gatactctca tagaaacgaa caggttgcat gacttgctgt
    13681 tcaagtagtc ccgcgattgt ttctttcttc attatttccg ttgttgtgtt gcttatgata
    13741 ggaaaagaag gttcagagaa ttgaatctga cttaggtcct tcgccagttt ttttgctgcc
    13801 ggttctaaaa tagctgtatg aaaaggcccg ctgacattta gagggatcat tcttttgaac
    13861 cctttttctt ttaatagtaa aacagcttcg tctactgctt tttcttcgcc accaatcacg
    13921 atctgctgag gtgtattata atttgcagga gatacgatgc cgtacttgct agcttcatga
    13981 cagctttcct cgatcgtttc aatcggcgca ttcatcactg caaccatttt cccgcttcca
    14041 gctggggcag cttctgtcat ataagcgcct ctttttgcta ctaacgcaac tgcttcagtg
    14101 aaggatagcg cattactagc tactaatgcc gaatattctc ctaaactcag tcctaaagct
    14161 gcatctggga tgattccatg ctccttcaat aagcgataat acgcgatgct gactgttaga
    14221 atagctggct gtgtatattg cgtctgatcc aaacgttcat tttctgtaaa gcaaagttca
    14281 gccatatcgt agccgagaat ctcacttgct tcatcaaatg tctgctttac aacggcttct
    14341 tcatataaat cttttcccat accttgatat tgtgcacctt ggccactaaa taagaatgct
    14401 gttttcatgt ttttctccaa tatatccaga atatatgttt gattcttcct attcaaaaag
    14461 gccgagacat tcctatttca ggaaatgtcc cagcccccaa cctaatttta atgattatgc
    14521 agtttgtttt tctacgtaag tcactaaatc ttgaaccgtt ttgattccgt cttctgtttc
    14581 gattttcacg tcaaattcat cttcaatttc attgatgatt tggaataaat ccaagctgtc
    14641 agcttctaag tcttcttgga tgtcagtagt caatttgatt tcttcagttt cttttcctaa
    14701 ttcttctgcg atgatttctt gaattttttc gaataccata ataatatatt cctccgatat
    14761 tgtttgcttt ttatttttat aatttaacag tctacaactc catgagaata cttccccatg
    14821 ttaaacctcc gccatagcct gtaaatatta ctttctgctg ttttcctaag gtgatctttc
    14881 cactagaaac tgcttcatcc aataagatag ggatgcttgc agcagatgta ttcccatatt
    14941 tatccatatt ggtcaggaac ttttcttgcg gcatctttac ttttcgggca attttctcaa
    15001 taattctgag gtttgcctga tgcaaaagca gataatcaac tggtttgtct ttcatgactt
    15061 cttggatatt gtttgcaacg tctcgtacgg caaaatcaaa aatatctctg ccagtcattt
    15121 gcaaataata cgaaccttct gaatcttctg aatagaacgg atttttattt tgctggtagc
    15181 cagcggtcaa tgagctactt ctggtaccgt cagctgctaa tttttctttc agcaattttt
    15241 tctgtggatt cgcttctaag agaatcccac ctgctccatc gccaaacaac acagcagtcg
    15301 tacgatcaga ccaatcgatt agcttagata gtgtttcacc accaatcacc agtcccttag
    15361 ttgagccgca gcggatcatt ttttcagcca tgcttaacgc ataaacaaat cctgagcacg
    15421 cagcactgat atcaaaagcg atagcatgag ttgctcccaa tttcccttgt acttgtgctg
    15481 ctacagatgg tgtcgtgtaa tcgggcgaca ttgttgctac taaaataaaa tccaaatctt
    15541 tagggtttag atttttctgt tttatcaatt tttccgccac taaaatgcat aaatcagaag
    15601 tctcctgatt tgttacacat cttcgttcac gaatacccgt ccttgacgaa atccattcat
    15661 cagaggtatc catcatcttg gaaagatcat tattagtaat aatcttttca ggaaggacgc
    15721 tggctgttgc agtaatcatt ccaaattgtt ccattaaata tcctcactta tagtcttgca
    15781 agaagtcgtg caagtttttc agtgcatcca gcaaggcatg ctgttcctcc gtagccattc
    15841 cgtctaagat gttcttgacc atttcacgat gaaaatgctg atggacccga aatagtaact
    15901 ttcctttttt cgttagtccc aactttacaa cgcggcggtc atcttcactg cggatacgtt
    15961 cgacatagcc tttttttact aacttgttaa tagcagtggt caaggtccca acagtaattg
    16021 atagttcttt ggctacttct gaagttgttt ttttcttgta catcccgatt gcctcgatcg
    16081 tgtgcatctc tgtgattgac agatctttga attgagattt tttcagctca gactcttcga
    16141 ttgtcaaaat atcattgaag acactaacaa gataatcatt tactgttttt aaatctggtt
    16201 ccatgttcca ccgtccacta attagtttga ttatcaaatc ttttgatgtt caaactatat
    16261 cttaactttt ttcaaaatgc aaccttaaaa gatcattttt ttac
//
LOCUS       DADVXU010000063        16140 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.066, whole genome shotgun sequence.
ACCESSION   DADVXU010000063 DADVXU010000000
VERSION     DADVXU010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.066"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(267..1994)
                     /locus_tag="KB953_001887"
     CDS             complement(267..1994)
                     /locus_tag="KB953_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HBB6866859.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(1987..3180)
                     /locus_tag="KB953_001888"
     CDS             complement(1987..3180)
                     /locus_tag="KB953_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HBB6866860.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3367..4011)
                     /locus_tag="KB953_001889"
     CDS             complement(3367..4011)
                     /locus_tag="KB953_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBB6866861.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4105..4239)
                     /locus_tag="KB953_001890"
     CDS             complement(4105..4239)
                     /locus_tag="KB953_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HBB6866862.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4241..6373)
                     /gene="feoB"
                     /locus_tag="KB953_001891"
     CDS             complement(4241..6373)
                     /gene="feoB"
                     /locus_tag="KB953_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBB6866863.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6370..6843)
                     /locus_tag="KB953_001892"
     CDS             complement(6370..6843)
                     /locus_tag="KB953_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBB6866864.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7244..7468
                     /gene="nrdH"
                     /locus_tag="KB953_001893"
     CDS             7244..7468
                     /gene="nrdH"
                     /locus_tag="KB953_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HBB6866865.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            7627..9786
                     /gene="nrdE"
                     /locus_tag="KB953_001894"
     CDS             7627..9786
                     /gene="nrdE"
                     /locus_tag="KB953_001894"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBB6866866.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            9836..10801
                     /gene="nrdF"
                     /locus_tag="KB953_001895"
     CDS             9836..10801
                     /gene="nrdF"
                     /locus_tag="KB953_001895"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBB6866867.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(10890..12029)
                     /locus_tag="KB953_001896"
     CDS             complement(10890..12029)
                     /locus_tag="KB953_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBB6866868.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12338..13051)
                     /locus_tag="KB953_001897"
     CDS             complement(12338..13051)
                     /locus_tag="KB953_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBB6866869.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13305..14006)
                     /gene="nagB"
                     /locus_tag="KB953_001898"
     CDS             complement(13305..14006)
                     /gene="nagB"
                     /locus_tag="KB953_001898"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBB6866870.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14240..15772
                     /locus_tag="KB953_001899"
     CDS             14240..15772
                     /locus_tag="KB953_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBB6866871.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(15963..>16140)
                     /locus_tag="KB953_001900"
     CDS             complement(15963..>16140)
                     /locus_tag="KB953_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB6866872.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         4996 a         3338 c         2827 g         4979 t
ORIGIN      
        1 ataaactatg tatattcttt aatatttttt gagaatgcac ttcgtacata ataaacttta
       61 atcacattag tctttcatac ctacgatcga tctctttgac tcgcacccat aaattcataa
      121 tagtttgtcg gcgtctttga acgtttcgaa gatgagctac gcattctctt tttttataga
      181 caaaaaagat agagacgaac atggagcttc caaatttgga tatttgatcc aattttggaa
      241 gctccattta tttcgtgtct gtctttttat tttttccagc tttcgactaa ttcttggttc
      301 tctttgatcc agtcttttgc tgcttgctgt ggatcttttc cattgttgat ttccaacata
      361 actttttcca tatctttttc tgaccaatgg aaattgtcta atactttgta agcttctggc
      421 tggtcttctt tcaatccttt tcgaaccatc gtatggatct gctcttcgct tcccattccg
      481 ttttcaggat cttctaagta tttcagatca tatttggcaa acatccaatg aggtgtccat
      541 cctgtaacta caattggttc gtgttttttg atcgcttgac ctagtgcaac ggtcattgct
      601 cctgaagaag atgtctctac tttccagtct ttcaagttgt catatttttg gatcgtgttt
      661 tcagcagccg taactacacc agctcctggc tcgattccta tgattttctt acctgcctga
      721 tcggttaact catcgatcga atttacatcc atgtaggctg gcactgctaa accaactttt
      781 gctcctgtca aattggcacc taggtcttcg acttgatctt tatattgtgc atattgtgaa
      841 ccatgagttt taggtagcca agctgaaacc atggcatctg actcgccatt tgaaacagat
      901 ttccacatga ttgaattatc taatggcgtc atcgttacat catagcccat ttgtttcaat
      961 acttcaccga ctacatttgt tgaagcaact tctgtatccc attcaacata agataagttg
     1021 atacgttttg tttctttggc agatgaaaaa ctccctatac caatcgcacc taagatcact
     1081 actactacag caccaatgat cagtccttga cgttttttac ttttctgtgt tgttgctcct
     1141 gcagcttttt tattgtttgg ctggttcaaa tgctgtgtaa aacggtcaat gatgattgct
     1201 aagataacta atgcaacccc gttgacaaat ccattcccga cttgtgctct ttgcaatgct
     1261 gataatacgc cgcgtcctaa gccgggtgca ccgatcatag aggcaataac gaccattgaa
     1321 agtgacagca ttgttgtttg attgatccct gccatgatcg tacttttagc taaaggcaat
     1381 tctagtttga atagtttttg acgacttgtg ctcccgaatg aatcagaagc ttctactaat
     1441 tctttaggaa cttgtcgaat ccctaaattc gtaaaacgaa cggttggtgg cagagcaaag
     1501 atgactgagg caaatacccc tggaaccatc ccgataccga aaaatgcaac tgctggaatc
     1561 aaataaacga atcctggcat tgtctgcata aagtcaagga tcggtttgat aatactttgg
     1621 gctttactgc tttttgccat caagatccct aatggtaccc caatgatgat cgagatcaca
     1681 ctagataaaa gaacaagtgt gacagtattc atcaaatcat tccatagacc ttggttataa
     1741 ataaaccata gaccgattaa tgtgaacaac gtaagtcctg gacgtttctt agaaataaag
     1801 aaagctgcta ccgtcaataa caagataaac aaaggagctg gaatcactaa cagtaaattt
     1861 gtaatccccg acatcactgc ctgtccaatc gtttgtaaaa atgaaaatag gccagaaaaa
     1921 gttgtcgtaa accattctgt gatattttct acccagtttg ccactggtaa ttgatagttc
     1981 aatagattat tcatgttcac tcacctcgct tgtttctgct aatgcttcaa tcacactacc
     2041 tcggatgacg acacctttta attttccatc ctctacaact gcaagtggag tcggtgagtc
     2101 ataaataata ttgaaaatat cacttaccac catgtctttc ccaatcaccg tcacattttc
     2161 atctaggaag tcgatcaatg gacgtttttg cttcctagct tctaacgctt gatctgcagt
     2221 gatgatccct ttcaggtgtc tctttctgtc aacagccatc agcatgctga cttcttcatt
     2281 acgcatcctt gtcaatgcaa cgtttggtcc atcagattcg atattcgtcg tcaatgcagg
     2341 taccatgatg ttttgagccg taagcacttt ggaacgatcg acttcttcca caaattcacg
     2401 tacataatca tttgctggat tggtcaatat ttcttcacct gttccgatct gcatgatttc
     2461 gccgtctttc atcaaggcga ttctgtcccc aatccgtaaa gcttcattca aatcatgggt
     2521 aatgaaaata atcgtttttt gaacattggc ttgcaggtcc agtaattcgt cttgcatttc
     2581 tcgccggatc agcggatcaa gagccgagaa cgcttcgtcc atcagcaaga tctcaggatc
     2641 atttgctaaa gctctggcta ggccaacccg ttgttgcatc ccaccagata actgactagg
     2701 atattggtct ttaaatgaaa gcaaactaga attttctaat gctttttctg ctttttcttg
     2761 tcgttcttct ttaggtacac cacggacttc taacccgtat tctgtatttt ccaagatcgt
     2821 tcgatgtgga aacagaccga agttttggaa aaccatattg attttatgac gacggacttc
     2881 tcgcaattct tctttgctta acttcgatac atcttctcca tcaatataga tactgcccga
     2941 agttggctca atcagacgat tcaatagacg gatcaatgtg gatttcccac tcccggaaag
     3001 ccccatgatc acgaagattt ctccttcttt tacgtcaaaa ttcacgtcat acacaccaac
     3061 cgttgcaccg gttttcttta aaatctccgt tttcggctgg tgatcattca tcatatctaa
     3121 agcggcttgt gtctttttac caaatatctt tgttaaatgt tttacttcta cttttaccaa
     3181 acaaattctc cttttttatg taaattcatt tttctttctg aaaaaagtga ccacactatg
     3241 ttaacatcat gtagtcactt tgtcaaaaat gaaagaaatt tgcttgtcca aactgaaaaa
     3301 aacttggcta aatctttgta aataaaaggt ttagccaagt taatattttt ttaatatttc
     3361 acacaatcat tctgcaagct gatgctccgg gtaaaaaaag tttgtcatac gctgtagtgt
     3421 atattcattt ccaataaaat gaacgatgtc ccctgccgta aatttagcat aggggccagg
     3481 tgaaacaagc agttcttcat tatgagaaat tgcgataact gtggcgccag tcgcctgcca
     3541 gatattcaac tcgctaattg aacggtccag atgcattgct ttttctgata aagataattc
     3601 aaacggaagt aaaggattca tcttatgtat tcgcttcgtt tgattgacca gctgatccaa
     3661 taattgagaa aaatgattca gctcatcttg ttgcttctcc acacttgcca tgatttctgc
     3721 ttttgtatct tggatggatt gtacatcttg atattgcaat aggaaatctt ttgctttttc
     3781 cttcgaagca acaaatgccc cacttccatg ccgtacttcc atgattccaa gatctaccag
     3841 cacattgata gccttgcggg tagtctcagg agaagcattg tacgtactgg caagtgttga
     3901 gcgggcatgg atcttctcac ccactttata tcggttttca acgatacgtt cggctaaatc
     3961 aacagcaatc tgctggtagc gcgggcggac gatttttttc tttccagaca ttttctttcc
     4021 tctttctttt tttactttcc ctcatcttac tatgctctag caaaataagc aaaaaagggg
     4081 agaatagcct cttgcaacga ctgatcatcg cttgatctgt tcctttttta ctgggcaagc
     4141 tgtttgacag tcatcacaat tcttaccgga ttttaaacga gtatagacaa ttgcgcctgc
     4201 acttccaaag attaacacac ttaataagaa tgtcgccatc ctattcctct ccttctaatg
     4261 acaatatagt ataaaactgg ttcttggatt ttggctgacg gaacaaaaca taaacgagtc
     4321 ctatcatcag taaaatagca agatacgtcc ctaacgctac ctgtccaccc tccaataagt
     4381 gtcccaactg ataaatcaca aaactaacta aataagctaa accgcattga tagcctatgg
     4441 cgatccaagt ccatttcttg gcattcattt cccgatggat agccccaatt gccgcaaaac
     4501 aaggcgcaca aagtaaattg aaggctaaca atgaataagc tgctgcggga gtatatgtgt
     4561 gttgtagcac ttgccagatt tctctgccat tttcggatac ttccgcatga ccaaacaaaa
     4621 taccaaatgt gccaataata ttttctttag cgaccaatcc tgtaatcgct gcgactgttc
     4681 cttgccagtt tccccaacca agcggctgaa aaacaactgc caggattcgc ccgaaaaagg
     4741 ctaagatact ttggtcttca gaaacaggtt gagcataaaa attataagaa gagctgaacc
     4801 agatcacgat actagaaaca aagataatcg ttcctgcttt tttaacaaaa gacttgctgc
     4861 ggtcataggt ttgtcttagg accgctgacc attgaggaaa atgatatgcg ggcaattcca
     4921 tgataaaagg agctgtatct ccagcaaaca aacgtgtttt tttcaagctg attccagaga
     4981 aaataattgc agtcatacca agaaaatagg cagaaggagc tacccaagat tgatgaggaa
     5041 aaaaggcccc tgctactaag gcaataatcg gcaactttgc agaacaaggc atgaaggttg
     5101 tgaccatgat cgtcaaacga cgatcttgct cgttttcaat cgtgcgactg gccatgacgc
     5161 ctggcactcc acatcctgta gcaatcagca taggaatgaa ggattttcct gaaagaccga
     5221 atttcctgaa aagtcgatcc atgacaaaag cgatcctcgc catatatccg cagtcttcca
     5281 gaaagctcaa gcataaaaac aatataatca attgcggtag aaatcctaac actgctccca
     5341 cgcctgcgat gatgccgttc aagatcagct cctgcatcca tcccgctact tgccattcag
     5401 ctaacaatct gccaacatgt tctggtaacc atgtaccaaa caatgtgtca ttgatccaat
     5461 ctgtccccat tgttcctact gtttgaatag ccagataata gatcagccac atcacaaatg
     5521 cgaagatcgg caaggctaac caacgattcg ttgcaatctg atcgattttg tcactcatcg
     5581 ataattggaa agaatcattt gacacaacac agagtgtaca tagttgggta ataaattcat
     5641 aacgttcatt gaccagaatc gtctcactat cgtcgtccaa tagtttctct gttaactgga
     5701 tcagttgctc gatctctttt tgctgagaaa ctgctatctc gtaatcctct cttaccttct
     5761 gatctcgttc aaaatatttc attgcatacc agcgtttctt gccgctttgt tctcctagta
     5821 catcttcaat ttcatgtaaa gctgcttcta aacgtggatc atatgtacag ggaacaggaa
     5881 tagaaggccg cttttttgca gactcctgaa tcttttcgat tagtttttcg attccttgct
     5941 ttttcaatac gctgattggt aaaactggta caccaagacc ataggaaagc ttttctagat
     6001 tgattttttt tccttctttt ttgatgacat ccatcatatt cagaccaatg atcataggaa
     6061 tgtctaaatc caataactgc gtagttaaat aaagatttcg ttctaaattg gtagcatcaa
     6121 taatgttgat gatcagttca ggctgttcct gaaccaaata atctcttgca atcacttctt
     6181 ctggtgtata aggagacaaa gaatagatgc cgggtaggtc ctgaatatga atgttcggat
     6241 tttttttcaa cgatccttct tttttttcta ctgttacgcc cggccaattc cctacatatt
     6301 gattcgaacc agtcaagaga ttgaacaagc tagttttccc gctgtttgga tttccgacta
     6361 acgcgatatt cattcttttt cctccctttg tatcaagatt agttcggcct ctgactttcg
     6421 taacgtcagt tcataccctc gcactgtcag ttcgattgga tctcccagag gggctagttt
     6481 tcggataact atttttacat ttcgcgtcaa tcccatatcc attaaccgtc gcttcactgc
     6541 gccttgtccg tggattgcta cgacgatccc agtctctcct gcttttaatt gatcaagaga
     6601 agtccagctt tcagaggaag cagtcttttt attgacaaag atttgaccta gtatttcccg
     6661 acttactgcc actcgatgac tttttaccag taaaatggca ttgttgcctg ataaattgac
     6721 gacgacgatt ttacttccgg caacgatccc gatattttgc aaatggcgtt ttgttttttc
     6781 ttctgtttcg attcgttcga cttgataaac gaaattctgc tttgcctgcg ctaaggtctg
     6841 catattgatt cctctcttgt gaaataaatc atgtattaaa atcattcatt atatgttcta
     6901 attttcttac gtgttacgca aataaatgag aatcattctc acatagaata ctctcggtaa
     6961 ttgagaatgt caattatact tgtgatatta tgagcgaatc tttagtattg atagataaaa
     7021 atgagaaaaa aatatttctt catagttttc cacaattttt tctctattaa atttgctatt
     7081 ttcccacacg atacttgttt tcagctcttt tttcaaaaac acaaaactta gtattccaca
     7141 gtttccacaa cacaatatgt agtgtttgtt gtttgacttt tttactaaga gttttatact
     7201 atctattata gataataaga atgtgttaga ggaggcactc accatgaacg taaaaatctt
     7261 ttctaaaaat aattgtatgc aatgtaagat ggtcaaacgg tttttaacag aaaatcaaat
     7321 tgcttttgaa gaagtcaata tcgatgaaca gcctgaagca gtagactggt tgaaagcaca
     7381 aggattccaa agtgttcctg tcattacttc tgatgcagaa acagttatcg gattccgtcc
     7441 tgatcagttg cgtaaacttg ccagctaaca tgactaaaag aatcaaaacg tgatacatgg
     7501 atttgaaggg cttgagacag caccagtttt tgtcgcaaac ttccaaatcc ttttttaaag
     7561 cttaaaaaca atgcaatgat ttgcaatcaa gcatcatcaa atacgaaatg aaggttgggc
     7621 gtacgtatga gtcttaaaaa tttacaagat gtcagttatt tcaagctgaa taatgaaatc
     7681 aatcggccag tcaatggtca aatccctttg aataaggata aagaagcttt agttgccttt
     7741 tttgaggaaa atgtcaaacc aaatacgatg acttttccat ctattatgga aaaaatcaat
     7801 tacttgatcg atcaagaata tatagaagca gaattcattc aattgtattc accatcattt
     7861 attgaaaagc tatatcaatt tttggacgag caacattttt ctttcaaatc ttttatggct
     7921 gcttacaaat tttattctca atatgcgttg aaaaccaacg acggttccgc ttatctggaa
     7981 acttatgaag atcgagtagc cttcaatgcg ctttactttg cgaacgggga tgaagaatta
     8041 gcacttgctc ttgcagacga aatgatccat caacgatatc agccagctac accttctttc
     8101 ttgaatgctg gacgtaaacg tcggggcgaa ttggtgtctt gtttcttgac tcaagtcact
     8161 gacgatatga actctatcgg gcgctcaatc aactctgctt tgcaattgtc ccgtattggt
     8221 ggcggcgtag ggattacttt atcaaatctt cgtgaagccg gcgctcctat caaaggatat
     8281 gagggtgctg caagtggtgt cgttccagtc atgaaattat tcgaagatag tttcagctat
     8341 tctaatcaat taggtcagcg tcaaggagct ggcgtggttt atttgaacgt tttccatcca
     8401 gacattattg ctttcctttc agcaaaaaaa gaaaatgcag atgaaaagat tcgcgtaaaa
     8461 actctttctc ttggcgtgat cgtacctgat aaattttatg aattgacacg taaaaacgaa
     8521 gatatgtatt tatttagtcc atatagtgtc gaaaaagaat atggtgtccc attctcttat
     8581 gtaaatatta cagctgagta tgatcatcta gttgcaaatc caaacattcg caaacaaaaa
     8641 atcaaagcgc gtgatcttga aaatgaaatc tcaaaattac aacaagaatc tggctatcct
     8701 tacgtaatca atattgatac agcgaaccgc gagaatccaa tcgatggcaa gatcatcatg
     8761 agtaatttat gttcagagat tttacaagtt caaacgcctt ctgtgattaa cggcaaacag
     8821 gaatatgaag tattaggaac agatatctca tgtaacttag gctcgacaaa tatcgtgaat
     8881 ttgatgcaaa gccctaactt tggtcaatct gtgcgtacga tgactcgtgc attgacattt
     8941 attacagatg catctgatat cgatgtcgta ccaccgatcc aaaacggaaa taagctgaat
     9001 catacgattg gattaggtgc catgggctta cacacttatc ttgcaaaaga acaaatggaa
     9061 tatggctcag aggattcatt agactttaca aatatttact tcatgctgtt gaattattgg
     9121 acacttgttg aaagcaacaa tatcgctcgt gaaagaaaac aagttttcca taattttgaa
     9181 aaatcagctt atgctgacgg cacttatttc gaaaaatacg taactggaga attccagcca
     9241 aaatcagaaa aagtcaaagc attgtttgaa ggcattttta tcccaactgc tgaagattgg
     9301 aatgctttga aacaagcagt aatgaaagat ggactgtatc atcaaaatcg cttggcagta
     9361 gcaccaaatg gctcaatttc atatatcaac gatacgagtg caagtattca tcctatcact
     9421 cgcatgatcg aagaacgcca agagaagaaa atcggtaaaa tttattaccc agcgccttat
     9481 ctatccaatg agacgattcc gtattacact tctgcttatg atatggacat gcgtaaagta
     9541 atcgatgttt atgcaacggc acaacaacac gtcgatcaag gcatgagttt gactttattc
     9601 atgcgttctg aaatcccaga aggcatttat gaatggaaga caacaacgaa gcaaacgaca
     9661 cgtgatttga atattttaag acattacgct ttccacaaag gaatcaaatc catttattac
     9721 gtacgtacct ttactgatga tgcagaagaa atcggaagca atcaatgtga aagctgcgtg
     9781 atctaatatc atagatagat tcgcttattc atattttaat taaaggagca ttctcatgtc
     9841 agaaacatat tatgaagcaa taaactggaa tgaaattgaa gatatcattg ataaatccac
     9901 ttgggagaag ctgacagagc aattttggct agatacacgt atcccacttt caaatgacct
     9961 agatgattgg cgtacattgt ctcaattgga aaaagataca gtaggacatg tatttggcgg
    10021 tttgacactg ctagatactg tccaatctga aagtggcatg gatcagttgc gtaaagatgt
    10081 ccgcactccc catgaagaag ccgttttgaa caatattcaa ttcatggaat ccgttcatgc
    10141 caaaagttat tcttctattt ttagtacact gaatacgaaa aaagaaatcg aagaaatttt
    10201 tgactggaca aatacgaatc cttatctgca aaaaaaagct gagcgtatca atgaaatcta
    10261 caaacacggt acaccgctag aaaagaaaat tgccagcgtc tttttagaaa cattcttgtt
    10321 ctattcaggt ttttatactc ctctttatta tttaggaaac aacaaattgg ctaacgtagc
    10381 agagatcatc aaactaatca tccgagatga atctgtacat ggaacctaca tcggttacaa
    10441 attccaatta ggattcaacg agttacctga agcagaacaa gaaacgttaa aagactggat
    10501 gtacaatctt ctttatgagc tttacgaaaa cgaagaacgt tatacagaag aattatacga
    10561 tccaatcggc tggacagaag aagtcaagac attcctacgc tataatgcca acaaagcatt
    10621 gatgaaccta gggatggatc cactctttcc agatactgcc aatgatgtca acccaatcgt
    10681 aatgaacggg atttcaacag gcacaagcaa ccatgacttc ttctcacaag tcggaaacgg
    10741 ctatttgtta ggtcatgtag aagcgatgaa agacgacgat tatttgattg gattggaata
    10801 aggatttatt ttaattcaaa aatttttcga aaaataaaaa gagtttagcg tacagatatt
    10861 ttaaatgtac actgaactct tttttatgct tattcacttg tttcttccag cacttttgga
    10921 ctgtctaact tttccgaaac atgttccatt cttctcaata aatagatttt ccggatattt
    10981 aatattatcg tcttaatcac tgcatagaca ggtatgcaaa tcagcatgcc taatattcct
    11041 gcgacacttc ctgctccaat caataccaag atgatcgtca aaggatggat attcattgat
    11101 ttcccaaata acaacggctt gatcaaattt ccatctaatt gttggatcac taaaatcgac
    11161 aacgccatat acaaagcttg aaatggagag ataaacaaac cgacaatgat tgctggtacc
    11221 gctccaataa aagggccgac atacgggata atattcgtga ttccgcaaat gaccgcaagc
    11281 aacaagctat aaggctgatc gaatattctc attgccaaaa agctcattac accaataatc
    11341 aaagcatcta agatcgtact gctgatatag gcagataatg tttcgtttaa ttcttttacc
    11401 gtttggcgta actctttacg aatcgctttc ggaaagaagt gagacaacgc atcaataaat
    11461 ttgtgcccat ccttgaacat aaatagcaaa ataaatggaa cggtaaataa caaaataaaa
    11521 aattgcatca taaatgaaat gatttttgag acacttgcag ataaactgac taatatgata
    11581 ttgaacaaat tactaagaga taaatttgct gatgctaatt gttcttccaa attgaaattt
    11641 ttaaactctt cccgctgcgc caattcattt aaccatttgc ttgtttcatc tgcatacatt
    11701 ggcaagctct gggtcaattg aattgtctgt tccattaact gaggcactac attcatgaca
    11761 gttaaaacaa ctaaaatgat tacgaccaaa aacgtcaata aaaaaccgaa cactctcggg
    11821 atccgatgct tttcgagaaa aacaacgatt ggatcgaaca tataatagat aaaaccagca
    11881 acaagcaatg gtaaaaaaat tgccgaaagc attttcaata ttggactaaa aatattcggc
    11941 atttgtaaaa taaagtacac gccgatgatc gtgagtacta gctccactgt ccagaaaaat
    12001 aattttgatt gtttaaaacg tgattgcata catctcctcc aaggtccttt gatacttcta
    12061 ttctatcatt taacaaagag ttacgcgctg agattttcat ttgcttaaaa aaaacaaaaa
    12121 aaataaacat attttaatga gtagacgagt acttaaacaa aaacacggtt tggagtagtg
    12181 acaaaaatta tactgcagtc cctttttcca aataaacggt gttcaaaagc ttgcatcttc
    12241 gtaataagcc cagccaaacg aaaaatataa ggaactattt ttatttggct gggcttatta
    12301 cttgatgtag gacatctatt ttacaacctc agatcattta ttgcgcaata ctgactaaat
    12361 gcaactttgt gatatttttg tgtacggaca actcctcgat cacatcggca taagtgagcc
    12421 cttttggaag atcaatcgta tagatatttt tgtacactcg gtagtcatca acgaatttta
    12481 cgtcaaaatt cacatcttcg attacgatat ttttctcttc aaaataatcg gtaagaaaat
    12541 ctttggtttc ttttctatgg ataaattgga tttctaactt tttagtcgta ggtacatgga
    12601 tcacgcgttt cactactgtg agtgcaatag caatcccgat aaaactgatg atcgcaatgg
    12661 aatagtaacc catgccgatg gcgatcccta atccagcaat tgcccataaa gaagcggcag
    12721 tcgtaagtcc cgtcaccgat tgtttcgtga cgatgatcgt tcccgcgcct aagaaaccga
    12781 caccactcac cacttgagca atcagccttg cttcatctga acgaatcgtt ccagcaagct
    12841 gtggatactc taaagcatct cttaatgcac cactagcgat ctcaacttgg atcaatgcaa
    12901 tgatacatgc tcccatacaa actaaaatat gcgtacgcat acctgctgga cgatttttat
    12961 attgtcgttc aaaaccgatt gctccgccaa atagcatagc gatccctaaa cgtaaaataa
    13021 tctctggaat tgtcaatacc atatcattca tctaaacacc ctccagtcca atatatcatg
    13081 tctgaaagga gcagacaaaa aaagtgcaat ttttacctct tacgaaaaaa ggactgtgac
    13141 aaaatttttg tcatagtcct ttttatgaat aaacggtgtt caatcagctg accctcggta
    13201 ttactcaaca aacggcaaaa tatggggagc tatttttcct ttgtcttctc taatactcag
    13261 gtcaaaacgc tgatttcaca acctctttag cctactattg aaaattacaa cttacttgct
    13321 gcttcttcat cgatgatcac aacaacattg tcgtgatttt gcaatacact tgctggcaaa
    13381 tgattcgtta caggtccttc aatcatccct ttgattgcat cagctttgct ttcgccaaat
    13441 gccatcaaga cgatttcttt acttttcata attgaaccga ttcccattga aacagctttt
    13501 gttggtacat cttctgcttt atcgaagaat cgtttgtttg cattgattgt tgattctgtt
    13561 aagtcaacta ctgaagtcgt tccgtcaaat gaagcaccag gttcattgaa accgatatgt
    13621 ccattttgcc cgattcctaa gatttggata tcgattggat gtgcatcgat gatttgatca
    13681 tactttttgc atgctgcttc cagatcttct gcttttccat ctggtacaaa cgtttctttg
    13741 aatggttttt tatcaaataa ttggacatcc atgaaatgac ggtagctttg ttcatctgaa
    13801 ccgcctagtc caacgtactc atctaagttt acagaagtca tttcactgaa atccaaatcg
    13861 cttgaagtca tttctttata taaagtaact ggtgtgcttc ctgttgccaa tcctaatact
    13921 tttgcacctt tgttcatacc ttctttgatg atttcaaatg ctttttttcc gccttcttca
    13981 gcgtttttta cacgaatgat ttccatagtg attgctcctt tatattttgg tatagcccaa
    14041 ttatagcatt ctttttttct ttttacaata attagtatag accaaattgc atttttttca
    14101 ttttcttgac aataaatagg aatccgtgtt aggtttcata agatggttca atcaaacaat
    14161 ccatgttgca tgaaaaccaa gcaagtgatt cactgtggac gctttatgtc tgcagttttt
    14221 ttatgaaaaa ggagttatta tgagtatact gacattaaac aacattaccc agcaatttgg
    14281 cgacaaaatc ctctatgaag gtgtcgatct gcaattaaat gctggcgaac atcttgggct
    14341 gattggacaa aacggagccg gtaaaagtac attgattaaa atcatcactg gtgagatctt
    14401 accagatgac ggacagatcc attggcaaaa aaatattcac aaaggttacc tcgatcaata
    14461 tgtggaagtc gatgaaacat taacaatcga agaatttcta aaaactgcgt acgcaaaaga
    14521 atacgaaaag gaagaaaaaa ttgcttttct ttaccaaaca tatagcgaga ccttcgatga
    14581 ttccttgctg gaaaaagctg ggaaactcca aacggaatta gaccaaggag tgttttatca
    14641 aatcgacacc ttagttgctg agatgagcag cgggttgggg atcgatgtct taggtttaga
    14701 ttcactgttg aaaaatctaa gtggtggaca acgttcaaag gtgattttag caaaactact
    14761 tttagaaaac ccagatgttc tgatcttaga tgaaccaacc aatcacttag atgatcagca
    14821 tatccaatgg ctgacgcagt ttcttcagga ttttgacggt gcgtatatcg tgatttccca
    14881 tgaccaagaa tttcttgatc agatcaccac ccatatcgca gacatcgagt ttggaaagat
    14941 tactaagtat actggccatc taaaacaagc attgaaacaa aaggaacaaa atcgcgaaag
    15001 ctatttgcgc caatatcatg ctcaacaaaa acaaatcgaa aagaccgaag cttatattcg
    15061 aaaatacaaa gccggttccc gatcaacgat ggcaaaaagc cgacaaaaac aattagataa
    15121 aatcgaacgg ctgacgcctc cagcttcagc aagtaaagct gtctttgatt ttccattctc
    15181 gcctattgtc acgacattag cagtggaaac aactgattta gtcatcggat atgatcgacc
    15241 gctacttgca cccatcaatt tgaccatccg ctttggtgaa aatattgcca ttcgaggctt
    15301 caacgggatt ggaaaatcta ctttattaaa aacactgatc ggtgagattg aaaaaataag
    15361 tggcgatttc cacttcccag ataacacaaa aatcaattac ttttcacaag atctgtactg
    15421 ggaaaatcca ttagaaacac cactacaata tttaagcagt caatttccaa aagcgactat
    15481 caaagaatta cgtcgccaac tagctaaagc agggttagtt aatcaattag caagtgaacc
    15541 tctcactaca ttaagtggtg gggaacaaac taaggtgaaa cttgcacagt tgacaatgaa
    15601 ccaaggtaat ttactgattc tcgatgaacc gacgaaccat atcgatcaag aaacgaaaga
    15661 aagcttacaa gacgggatac agaaatttcc aggaacagta atcatcgtct ctcatgaaca
    15721 agaattttat caagatcttg tgcaacgagt cattgagatt gaaccaaaat aaacgtttga
    15781 ttgaatagtt gaaaaaaagg agctgtgaca aaagttttgt cacaactcct tcttctgaat
    15841 aaatggtggc caattagctg accttcggta ttagctcaaa aaacggcaaa atatgaggag
    15901 ctatttttat ttggctattc ttattacttg atgcagaaca gcttttcaca acctcttttc
    15961 gtctattttt tattttcgag atttctcttt cctttaaaaa caactgtttg aaagaagaac
    16021 gcacatcctt gaaaaacaag cccgctaacc gttacaccta accagccata ctgctgccac
    16081 attgtcgtcc ctagcaatga accagccgca cctccaataa agtacataaa cataaataca
//
LOCUS       DADVXU010000064        15997 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.067, whole genome shotgun sequence.
ACCESSION   DADVXU010000064 DADVXU010000000
VERSION     DADVXU010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.067"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            226..894
                     /locus_tag="KB953_001901"
     CDS             226..894
                     /locus_tag="KB953_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBB6866873.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1324)
                     /locus_tag="KB953_001902"
     CDS             complement(1094..1324)
                     /locus_tag="KB953_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866874.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1321..1479)
                     /locus_tag="KB953_001903"
     CDS             complement(1321..1479)
                     /locus_tag="KB953_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866875.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKMKKKHA
                     NSLFLNNK"
     gene            complement(1451..2833)
                     /locus_tag="KB953_001904"
     CDS             complement(1451..2833)
                     /locus_tag="KB953_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBB6866876.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2989..4176)
                     /locus_tag="KB953_001905"
     CDS             complement(2989..4176)
                     /locus_tag="KB953_001905"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HBB6866877.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4183..5214)
                     /locus_tag="KB953_001906"
     CDS             complement(4183..5214)
                     /locus_tag="KB953_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBB6866878.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5260..6684)
                     /locus_tag="KB953_001907"
     CDS             complement(5260..6684)
                     /locus_tag="KB953_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB6866879.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            6859..7764
                     /locus_tag="KB953_001908"
     CDS             6859..7764
                     /locus_tag="KB953_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBB6866880.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7855..8532
                     /locus_tag="KB953_001909"
     CDS             7855..8532
                     /locus_tag="KB953_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBB6866881.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8529..8699
                     /locus_tag="KB953_001910"
     CDS             8529..8699
                     /locus_tag="KB953_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866882.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8813..9709
                     /locus_tag="KB953_001911"
     CDS             8813..9709
                     /locus_tag="KB953_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBB6866883.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9764..11134)
                     /locus_tag="KB953_001912"
     CDS             complement(9764..11134)
                     /locus_tag="KB953_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBB6866884.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11372..12118
                     /locus_tag="KB953_001913"
     CDS             11372..12118
                     /locus_tag="KB953_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBB6866885.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12115..12954
                     /locus_tag="KB953_001914"
     CDS             12115..12954
                     /locus_tag="KB953_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBB6866886.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12955..13905
                     /locus_tag="KB953_001915"
     CDS             12955..13905
                     /locus_tag="KB953_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HBB6866887.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14046..14798
                     /locus_tag="KB953_001916"
     CDS             14046..14798
                     /locus_tag="KB953_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866888.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14817..15968
                     /gene="queG"
                     /locus_tag="KB953_001917"
     CDS             14817..15968
                     /gene="queG"
                     /locus_tag="KB953_001917"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBB6866889.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5193 a         2996 c         3222 g         4586 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt tgataccgtt
     1381 gactagttct tccatcgcaa aagcagtccc ccattttaat ccaaatggcc gatctcgata
     1441 ctcgttatag tcaatgaatt tttttgctgt ccgtctcatc ttttccacct tctaatccac
     1501 tcataccacc tgcatgcccg ccaacgagag aactacgtgc gatggcgcgt cctccttgca
     1561 agagactatg cgcatggatg attgattgaa agccgtattt ttttctaatc gtatcgacaa
     1621 caaaatcaac tttcgcttca tttatctgct tttcaggatc ttcaaaaagg ttaagctgta
     1681 aacctgtcgc gtatacgagg tcggaacagt tcacgcctac acgccgaata tcctggtagt
     1741 ggtaatgctg gtggaaaatg gtcaataaag catctgcaag atctctgcta gcatttgtcg
     1801 cttcgatttt catttgctgg tgaaacccgg tcttgccatt tggatcaaga tagcctaaag
     1861 agtatcctac ccataagctg actacttgtg tttttgctgc agatcgtctc aagcgagtgc
     1921 cgacttggtc ggccatttct ttgataacga tctcgatttc ttctcgtctt gtataatctc
     1981 tatctaggat ttgactattt ccgatacttt ttgattttgg tgtatattct tgtcccaaaa
     2041 aacttcgatc gatgccccaa gcatgggcat aaagctgagt accgatgacc cctagcttgc
     2101 ttttcaagtg ataataatcc gcgtgagcta aatcataaac agaatgtata cccatttgtt
     2161 tcagacgttt tgctgttcgt ttgccgattc cccaaaagtc agtgatatct gagattttcc
     2221 ataacttttc tggtacatct tcataacgcc attcagcaaa catttgcttt ccgtcttttg
     2281 agccattgtc taaggctagt ttagcaagta gcgggttatc gccaattcct atagtcgtgt
     2341 agattccggt ctgctcaaac acatctttat ggatcagttt agccatgtcg tacgcatttg
     2401 ttttaccaaa gagtgccatg ctatttgtta catcaaggaa gctttcatcg acggaataga
     2461 catgatggtt cgcttcgtct gcatattttt tatagataga attgatttct ctgttctttt
     2521 ccatataata ggccatcctt ggggcaacaa tatataaatc acttggatag gggaagggaa
     2581 gatctctggc acggctgata ttggtgatgc cgtaagcttt ttttgctgca ggactggaag
     2641 ccaagattaa tccgctaccc cgtgtttctg tcgaatcgga cggataggac atgactacta
     2701 atttagttgt aaggggatca agatcctttt caacgcactc tacggaggcg taaaaagatt
     2761 tgcagtcaat acataaaata tctcttgaag gttctttttc ataaacaaat accggatttt
     2821 gattcgccca cataacaatc acctcttcaa cacaattaga acacacgttc tctttccgtg
     2881 tcaataaaaa acagcaattt tcaaatacga gcaaaaggct cacatttgga aattgctgag
     2941 aggttatttt tatctatttg tttttagctg agatcgttga tttttttatc aaacatgaac
     3001 agcttctgga tcgatctttg ttgtcccatt tccaatgtgt gcaacataaa attccggagc
     3061 atagccgaca actttcatgt attcatcata gacattgttt ttgaaggcag gtatattttc
     3121 ttctttgaca agagcaatcg cacagccacc aaatccagct cctgtcattc gtgcacctaa
     3181 gacaccttct tgtttttgag cagcatctac tagtgtatct aattcgatcc cagttacttc
     3241 ataatcatag cgaagagagt ggtgagaatc atttaacagt tgaccaaatg cttctaagtt
     3301 cccagcttct aattcagctt ttgccttcaa agtccgctga ttttcagaaa cggcatggcg
     3361 agctctgcgt atcaatgtat catcgccgat ttggtcaata gttgattcaa attcttcttc
     3421 atctaagtct cctaaagaag agataggcaa agttttttgc aaacgtgcca aggcttcttc
     3481 gcattcgcta cggcgttcat tgtattttga atctgccaat tcccgccgct tattcgtatt
     3541 catgatgacg attgcataac catctagttt cactggaacc atttcgtatt tcagcgtatt
     3601 tgtatctaat aagattgcat gatctagctc accgaagccg atggcaaact gatccatgat
     3661 acctgaattg acaccgatga attcattttc tactttttta cctgtttgga ctagttccaa
     3721 gcgatctgtt tttaaatcaa acagatcttc taatacaaca cctactaata attctaatga
     3781 agcgctactg gaaagtcctg caccattagg gattgttcct tccactaata actcaaaccc
     3841 atgatcgatt gagtgtccag cttcttttaa tttaaggacc attcctttta cgtaatttgc
     3901 ccagtttgct tttttatcat attctagctc atctaaagta aaactaatga caccgatatc
     3961 gtcaaagttt gtggaataaa ctaatatttt attatcgtct cgaggtgctg ctacaccttg
     4021 cgttccataa gtgatggaag cggggaagac atggcctcca ttgtaatctg tgtgttcacc
     4081 aatcaaattg atgcgtcctg gtgagaagta ttgtgtagtt tttttaggtc cgaatgtttc
     4141 ggcaaatttt gtgtttaagc ttcttttgat ttccatgatg agttattcct ttcttgtttt
     4201 gattttgaat tctgttactg tttcaaatac tgatccggct tttaaatgaa tcgaaccaaa
     4261 tgctgagaag cgctctgcac ctggagctgt ttgagtctca aatgtgatgc caccgtgatg
     4321 tgccagtttt cggttgcgca tttctggtgt ttctgtgccg aaatttgcag tgaaaataac
     4381 cacactagaa gcatctgtag caatatctac ttggattctt ttatcggggc tagtcaagcg
     4441 agcggcttct ttacccaagc cggtttcttt taagaaaaat ggatgatcga ttccgtcaac
     4501 aagatttttt tgatccaaat cactagcaaa aacggacgct aattttttag gtatttgaaa
     4561 atcgaaagca cttcctgcag cattttcttt tacccctata ggaatactat ctgttcgcaa
     4621 aggagcataa gcttcgctat tgagccaaag ctcatgttga tcgattgact gagaagcatc
     4681 tcccgtcaag ttgaagtaaa catgatttgt cggattgaat aaagtatctt ggtcactggt
     4741 gccgcgagag gtcactcgcc agatattatc ttttgtcaaa gtatagcgga tttccacatc
     4801 cattgtacct ggaaaaccat gttctccgtc gggacttgta gtagtaaaaa tgacagaagc
     4861 ttcattttcg ccattcaaaa tgacataaga ccattttttt aattcaaatc caggagcacc
     4921 accatgcaga ttatgtccag tttttggatc tgtggctaat tggtaagttt ttccattgag
     4981 agaaaagcgg ccattttcga ttctccctgc ggtgcgaccg atagaagcac cgatatacgg
     5041 atctttttcg atatattctt cagcggtgtc aaatcccaaa actaattctc gatcatttga
     5101 ttttaaactg acgattctag cacctaaatc agagatagaa agtgctagct tgtttttgtt
     5161 ctccaaggtt atcaagtgat aaccttggcc aaaatctttt tctgtaatct tcattttcaa
     5221 gatcctttct taatgggaag gaactactgc atcgtgaagt taatcttcca ttgtatcagc
     5281 tggagccaat ttgttttcca atttctcaac gatttgtgca tgttttttct ctgtcaatgt
     5341 aatcttccaa gcaaaaataa tagcggagaa aatcatcaaa gcagcaggag catagaatgc
     5401 ataagctttg aaagtcgtta cgccatgagc agtgatatca gcagcagtag cattaccagt
     5461 catcccagaa gcgatagcga caaaccctac gatcccgtta gacaaagcac cagcgatttt
     5521 atctagtaaa gggcgaattg atagagtaac agcttcgtta cggataccat ttttccattg
     5581 accatattca acagagtctg tgatcgtcat caaagcggat aagaaaatca attgttgagg
     5641 gaagtagaac aatactagcc cgatgataac gattggcaaa cttgaacctg cgatcgtaaa
     5701 gaaaaagtaa gaagaaatca tcattgcgat ccctccaagg taaacgtagc gacgagagat
     5761 gacgcgtgta agcattggga atagtggaac agctactaaa ccaagtaagg cagcaactag
     5821 accaacgatc caaaattcag ttggttggcc aagtacatat ttgaagaagt agaataatac
     5881 ggcagttgtt gctgcattgg caatcgcata agcgatataa gcaagggcaa gccacatcaa
     5941 ctgatcattt tgagcaactg ctttgaacac atctttgatc ccagcttttt cattttgctg
     6001 acggatcgca gtatcgactt cttttgtgcc caaggcagtg ataatcgcag taccgcctga
     6061 aataacagca gcaatcacac cgaaccagaa ccagcctgaa gcgccttcaa tatgtttatg
     6121 agtaacaaga taggtaaaga aagtaacgat agggacaacg actagagtag taccatttgc
     6181 acctagagaa gagccgaaac gagcaaacgt agcaaatttt tcacgttcct ttgtttccgt
     6241 actcaaagct ggaatcattg accaaaaagc gatatcctta aatgaataaa aactatccaa
     6301 taagatgaaa acgattgcaa ataatatgat aaataaagtc gtattatgag tagctaagcc
     6361 aaagaaattg gtaaagattg ctaccaagag tacggaacta acaacgccgc cgataactaa
     6421 ccatggtttg aatttaccgt attttgtacg agtattatcg atgatgccac caatcaaagg
     6481 atcaaaaata atttcaacta agcggatacc tacaactaag cttgtaactg tagcaatcat
     6541 ttttgcattc gttgccttat ctgttcctgc gaacatctgg cttgtcacga ataccataaa
     6601 ataagtgctt aatgtaacat agaatgtgtc gtgtccaaaa gcgcctaaag cgtaggacac
     6661 acgttggatc attttgcttt tcataatctg tattcctctc ctgttcaata tataatacta
     6721 agtaaaccgg ttacaacgaa taacaaagat cttttaatga cttaaactta acaagaaatc
     6781 gcttcctttt taatatattt ctgtgacttt tatttaactt tttgttgcat tatactaaag
     6841 gagtaaggag ggatagacgt ggagcaatcg atttttttaa aaaaggaaca tcaacaagta
     6901 gaaaaagaat tgtattttga tttttgtggt ttctctaaaa cgatgccgtt tcattctttt
     6961 ggtcctgcta ttcgcaatga ttacatcctg catgtcgtga tggaaggcaa aggatcttat
     7021 catgtgaaaa atcaacaatt ccaattgaaa aaaggtgatc tgttcttgat tcgtccagga
     7081 gactcgacgt tttacttatc agatggagaa gatccgtgga tgtattgctg gctttcattt
     7141 ggtgggagtg ctgcagaaga aatcattcgg cagtcgcttt tcagagaaga ccaatacacg
     7201 atggtctctt ctgacataca aaaatatatc gacattattt tagaatgcat gaaagatacg
     7261 gaagaaacct tggaaaatga attacagtta acagcattaa cctaccgttt tttatcagtc
     7321 cttttaaaag atggcggaaa ggtcagcggt ggtgtacaaa aggttcattc ctctttagct
     7381 gttgaaacag tcaaatacat cgaggcacat tatgctgaaa aattgtctgt tgaggaaatc
     7441 gcccagcatt tagcggtcaa tcggagccat ttgtcgcgag tattcaaaaa tcatatgggt
     7501 accagtatca aggaatattt gatcggggta cggatcaatc gaggggcgtt tcttctttct
     7561 ctgacggatg aatccgtgga aagtattgcc tatcaagtag ggttcaatag tctagtcgta
     7621 tttagccgaa tgttcaaaaa gagtaccgga gaaacggcta cgaactatcg taaacgtatg
     7681 aaaaacgaaa attttaagaa tattgctttg gataccttga aaaaacagtt ggaaaaacaa
     7741 accgcagttt cttggacaac ctgattagtc tggttaaaat gacaagcatc ggcaaaagaa
     7801 acttgtggcg gttatgctac actgtaaagc gaagactaaa aaggaggaaa agggatgcgt
     7861 acacttatca tcattagcca tccaacgatc gaagaatctt taaatcagca atttttcaaa
     7921 gaagcttctg ctggtttacc agctacttgg catcatttgg aatcaagtta tccagacgga
     7981 aaaatagata tagagactga actacagctc ttggcccagc atgatcggat cattttccag
     8041 tttccgtttt actggtatag ttctcctgct catttaaaaa tttggcaaga taatgtcttg
     8101 gaacgagcag cacatgtatt aaaagggaaa gaactaggaa tcgtattgac aacaggtgtg
     8161 gcggaaaaag aataccaagc tggaggaaaa gaagaatata cgatttccga atttttgagg
     8221 ccgtatcagc gaatcgcaaa taaattccat atgacctatc ttccgccatt cgttttagca
     8281 caatttatgt atttatccca ggaaaaacgt tgggagaagc taattgctta tcagcagtat
     8341 ctctcattgg aaggcaaacc atcgctgact cagcggattg actggtttat tcaaagagta
     8401 caggaaaatc agaaaatgca ggaagaagaa tctgaaaaac aagcatatat cgtagaagca
     8461 ctgactgagg caaaagagca gatcgaagac ttgaccttta cactgcaaga aatgaaagga
     8521 acttcattat gacaaatgat tggctgatcc aacaattaga tgaattaagc gaaaaatcag
     8581 aatatcaaga ccgtgcgctc ttatatgaaa taaagaaact gatggctgag atagacgaac
     8641 gaacaatgca gctgcaagga gaaatcgacg gacaatcttg gaatcatctc aactggtgaa
     8701 attttcttat aaataacaaa gataaaaatg tgcacagcga caattatttg ctacaataag
     8761 aatatgaagc gtttgataac gctattatac ataaaaattg gtggtgaaaa aaatgtatga
     8821 tgaaaatgag tggggagaaa atgttcggat gacccgaaga agacagcaaa aacaggcagc
     8881 tcgtaagcgg atgatcatga ttggggcagc agttgccgtg tttttgatca tagcaggagg
     8941 agttgcgggt ctatattttc atggaaaaaa ttcgtcagaa gcgaaaagta cgaccataac
     9001 ggaaacaacg acaaagaaaa cagagacgaa acaaacaagt gagtcatcaa ctaaaacaaa
     9061 agagacacaa acttctgaag aaaaaacagc aaatggtttg ccagagcaac ttcaagcaat
     9121 cgtaaatgat tattcgaaaa agatcgaaga aaagacgcct gcattgattg aagaatacca
     9181 gacagaaatt cagggaaatc aagagggaat agctggttta tcagcagttg ctaaccaaaa
     9241 agcacgtgag cttcaagcaa tctccgacga agggatcaga aaattacgtg cagcgtatca
     9301 ggcagcagaa aataaagacg gtgtagactt agatactttg atcaatcaat tatcggctaa
     9361 ttatacgaat catgtcgcta agatttccga tatttatttg cagactagcg cctctcttca
     9421 agcagagtca acaagcacac aagacacaac atcttctgat tctgagacag cagagtcggt
     9481 agaagcttct gaaatgcaag ctcgaacaac acaagacaca acagattcct ctgaaactaa
     9541 taccaacagc gcgacttcac aggctgcgac aaccgtagtt ttggaaggag aaggtccaaa
     9601 tcaaatagcc gaacgaactg gcgtaccagt agaaacgatc ttgtcgctga atgggatgag
     9661 catggagaat tatttcctga ctcccggtga gacgctaaaa ttgaattgat aaaagcaata
     9721 aaaaagaacg aagcttccac taaggtgaag cttcgttctt tttttatttt ccggttcttt
     9781 tttcgcgtac aaagtaatgg atcgtcatgt aaagcaagaa aacgagtcca ataatcaatg
     9841 agattctcgt ttcaggattg ataaacatga aaatcaatac gatagctaaa aaagcaagtg
     9901 tcacataatt cgtataagga gatagtggca ttttaaaagg atgatcatgc attttatctg
     9961 cgtgcttctt tttgaagcga atctcgctga tcaaaatcac tacccaagga accataccag
    10021 gcaaaatact tgagctgtat acgaaaacga aaatattttc tgactggttg atgaacattg
    10081 gtagaagata atttaagatc agaccaatta agattccacc tgagattgct acgacaggat
    10141 aaaaaggcgc accgtgacga gatagtttca gaaatttttt agacatcttt ccatttatgg
    10201 caagtgtata gaccatacga cttgcgctga aaatccctga attgcaacct gataatgccg
    10261 ctgtaatcac gacaaaatta atcagtcctg cagcaaatgt aatgccgact ttagaaaaag
    10321 tttgtacaaa aggtgaacca atctcactca attgattcca cggatagata ctcacaatga
    10381 taaaaatagc accaatataa aaaatcaaga ttcgaccgat agtggactgg acagcttcaa
    10441 tgatcgtctt tttaggattc tctgcttcac cagcagtcat tccgatcagt tcgattcctt
    10501 gataggaagc aacaacgatc gataatgcaa agaaaaatcc tttgacgcct ccagtgaaga
    10561 atccaccatt tttccaaaga tttgaaatcc ctactgcctg accatgatta ccaaatccaa
    10621 ataagattac cccaagacct gcaatgatca tcaaaatgat cgttatgact ttgatgatcg
    10681 aaaaccaaaa ttctaattcg ccaaacgcct tgacagaaat aagatttgcc agcataagaa
    10741 acaagacgac aatgattccg ggaataattg ccggcaaatc aggccaccaa taattcatat
    10801 aagatcctac agcaatcact tcacttattc ctacgacaat atattgaaaa atattactcc
    10861 aagcggtcaa gtagccagcc acaggatgga tgtactcgct cgcgtaattt gcaaaagaac
    10921 cagtcgaagg atcaacgtaa agcatttcgc ctaaagcacg cataatcaaa tacaaaaaga
    10981 atcctgcaat cgcataggct cccatcactg agggccctgt ccaacgtatg gttgaggagg
    11041 cacccataaa cagaccgaca ccgatcgttc cacctagtgc gatcatttgg atatgacggg
    11101 cactaagact tcgagttaac ttttcttctt ccatacaaaa actcctttcg ttgttgagtc
    11161 aacaagaaaa aataatcaaa cgtttcaaca catgtttttc attattaggt tagccttatt
    11221 gaactcttga tgtgattcta cagaaaattt tcataaaatg cgaggaaaat tcgaaaaaaa
    11281 ctttttcatc attctacttg aaaatgcttt ccattttagt taaactatat ttaaataaaa
    11341 agttaggtaa acctaaaaag gagtgtaaga tttggaacgt acggccattg taattcaaga
    11401 cctttcagcc gcttatcaag ggaaaactgt tttgaaaact atcaacttga cgataaatcc
    11461 acaaaaaatt accggtatta tcggaccaaa cggtgcagga aaatcaacat ttatgaaatc
    11521 tttgctagag ttgataccag ctactactgg taaagtcacc ttttctggac agtcggtaaa
    11581 ctcagtccgt aaaaaaatag cctacgtgga acagcgaagt gaattggatc tttcctttcc
    11641 aattgatgta ttaggcgttg tacttttagg aacatatcca tctttacgaa ttggacaaag
    11701 acctgggaaa aaagaaaaag aacgtgcaag acaagctttg aaaaaagtag ggatggaaga
    11761 atatgccaaa agacagatca gcgaactatc gggtggacag cttcagagag tttttattgc
    11821 aagagctcta gcccaaggag cagaatggat ctttttagat gaaccattcg tagggattga
    11881 tgcgttaagt gaacgaaaga tctttgacat cttgcaggaa ttgaagaatt caggaaaaac
    11941 gattttgatc gtccatcatg atcttcataa agtagaagaa tatttcgatg aggttattct
    12001 tttaaataaa cagctgatcg cttccggtcc agtacaagaa gtctttacat cgaagaacct
    12061 tcaattggcc tatggtgaga tcatcagaca tttagtaaaa ggaggagaga aaaaatgatc
    12121 caatccttta ttgatggatt aatgaattat caattcctgc aatacgcttt ggtcacttcc
    12181 atgcttgtag gacttgcttc tggagtcatc ggttctttta ttattttacg agggatgtct
    12241 ttgatgggag acgctatctc tcacgcagtt cttccaggtg tagccatttc ttatatgttt
    12301 ggttttagtt acatctttgg agccactgct tttgggatgc taacagcagc agtgattggt
    12361 ttcgtgactc agcggagccg attgaaaaat gatacagcga ttggaattgt ctttagctcc
    12421 ttttttgcat tagggattat cttgatttca tatgcccaga gtgcgacaga tttgtatcat
    12481 attttatttg ggaatgtctt agctgttagg gaaagcgatt tgttgctgac agcactcgtt
    12541 agtggaatcg tcttgatatt tgtctttttc ttttataaag aattgaagat cacatcattc
    12601 gatccgacaa tggcaaaagc ttatgggctg aacacatcgc tgatccatta tcttttgatg
    12661 ttctttttga cattagtggc tgtagtcagt ttacagacag taggaacaat cttggtgatt
    12721 gccatgttga tcacaccagc cgccacggct tacttgctaa cgaaccattt actgaaaatg
    12781 atcattacag ctgcaggaat cggtatgcta agtgcagttg tcggtgtgtt tttcagttat
    12841 agttacaatt ggccatcagg agctacgatc gtgttagcat gtaccgcatt ttttatcctt
    12901 gcttttttat tttctccaac aaaaggaatt ttatttcaga aaggaagcag atagatgaaa
    12961 acaaaaaaat cactcttttt aatcttggca gttagtttct tggtattagc cggttgcgga
    13021 aagcaggctt ctgaccaagc ggatgaagga agtaaagaaa agttgtcagt cgtggctacc
    13081 aattcgatct tggcggacat ggcaaaagag gtcggaacag atcaaataga tatccacagt
    13141 atcgtaccag tcggaacaga tccgcatgaa tatgaagtac taccagaaga catcaaaaag
    13201 gcaagcgatg cagatgttat tttatacaac ggtttgaatc ttgaaacagg taacagctgg
    13261 ttcgataact tgatggaaac ggctaaaaaa gaagaaggaa aagattattt tgcagttagc
    13321 aaaaatgtag aacctcttta tttaactagc ggtgaagaac atacaaaagc agatccccat
    13381 gcttggctag acctatctaa cggaataaaa tatgtggagg aaatcgcacg tatattctct
    13441 gaaaaagatg cagaaaatgc gacactctat aaaaaaaatg cagaagcata tgtggaaaaa
    13501 ctaaaagaat tagatacaca agcgaaggaa agttttgctt ctatcgaaga gaacaaaaaa
    13561 ttattagtaa caagtgaagg ggcattcaag tatttttcac gagcatatga tttgccagca
    13621 gcttatatat gggagatcaa tacagaaagt caaggtacgc ctgatcaaat gaaagcgatc
    13681 attgatcaga taagagcaaa agaagtaccg gttttattcg tggaaaccag tgtagactca
    13741 agaagcatgg aacgggtagc aaaagaaacg ggtttgaaaa tctatgataa actgttcact
    13801 gattccatag caaaagaagg agaacaagga gattcttact atcagatgat gaaatggaat
    13861 attgaaacga tccatgaagg tttgtcacaa acaaaagaaa gctgaaaagt gcaaggtcta
    13921 gcttgaggag cagaccttgc attttttata agagatatga aaaaaaatta tgctgttttt
    13981 cgtcgtacag ataccttttt tgaagaaatt ccgttatagt tagagtaagg aaggaaggtg
    14041 tggctatgaa ttttacacgt aaagataaat atacgattaa agaagaagtc tttcgattga
    14101 tcagccagca ggaaaatacc gacaaggatc aagaccttca atttcttgaa gcagaattag
    14161 ctaaattatt catagcaaat caaaatgaga caacagaacc agatgaagag ctgtacatag
    14221 gcaaatggtt gtttgatgaa tttcagcctt tagatgaaaa taccggtatc ggttattttg
    14281 tgttcctttc tccagtcggc ttcacacctg gcgtattagt taaacaaaac gacgaaataa
    14341 ccgtcaaagc atggcctgca ttattttcag tcatcaaagc agaaggagta atcaaaaaga
    14401 tccagtcggc ctattataaa agtcaattta ttgccaacag caaattaatg aatatgtttt
    14461 ataatcgtct agtagaaatt gctaggcctg ggaaaatccc gcaaacattc acgtacacac
    14521 agcaaaaaaa tctctatgaa gaagtattgt attcccgcga tctgtctgag ttttcgccat
    14581 gggaagaggt aaggctccat gaactgttaa gtacaaaacc agtttcattg aattttttta
    14641 atgcattaga taacaaaaga aaattaattt caacaaatta ttgggaagta tttgctgcaa
    14701 gaaaaaaatg gatggaagaa aagaagattg ccgatcagca gatccagttt gtccagctga
    14761 aaaaagaaaa actatattat gggttataca gccgttaaca gcaagcgagg aagaccatga
    14821 cattaaaaga agaaatcata caagaaagca aacgattagg aattgacaaa atcgggttca
    14881 ccacggcgga accttttgat tcattaaaag aatcgttgga agaacaaaaa gcagctggcc
    14941 atacttctgg ctttgaacat ccaaatatcg atgagcgtct ttatccagaa atgacatttg
    15001 atcagccgaa atcgatcatt tccattgcac ttgcgtatcc tactaggatc catgaagaag
    15061 tgcctaggga tgaaaaacgc gggcagtttg ctcgtgcttc ttgggggatc gattaccatg
    15121 atatactcag agaccgtttg aaccgattga tcgaattcat ccaaacaaga gcagaaaaat
    15181 ggcaaaaaga agaggaatgg cgtctagctc cgcaagtaga tactggagaa ctgatcgatg
    15241 tcgctgtagc acagcgagct ggtttaggat ttattggacg aaacggattg ttgatcactg
    15301 aagagttcgg ttcatttgtc tatcttggtg agatcgttac taatattgta tttgagcctg
    15361 ataaaccagg agaatttggt tgcggtgact gtatgcgctg cgtgactgct tgtccgacaa
    15421 aagcattgct tggtgacgga agaatgaatg cccagcgatg tttgtcttat caaactcaga
    15481 caaaagggat gatgccggaa gaatatcgta aaaaaatgcg aaatgtcatt tatggatgtg
    15541 atatctgcca gttagtctgc ccttacaatc aaggaaaaga ttttcatttt catgaagaga
    15601 tggaaccaaa agtagacgaa atatatccaa aattaaagcc aatgctttcg atgtctaata
    15661 aagacttcaa acagcaattt gggcatttag ctggttcatg gcgtgggaag aaacctttgc
    15721 agagaaacgc attgattgct ttagctaatc taggtgatcg aacggcattg cctgagattt
    15781 cagaatgttt aacagatgtc cgccctgtca tcagaggaac agcagcttgg gctattggac
    15841 gtctaggaac aaaagaaccg gaaaaatggc ttgatgtatt agatgaaata ttagctaaag
    15901 aaacagaaga agtcgttata aacgaattgg aacatgccca gaaattattg cggagaaagt
    15961 taaaataaaa aaatagttaa cattattcaa agttttt
//
LOCUS       DADVXU010000065        15909 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.068, whole genome shotgun sequence.
ACCESSION   DADVXU010000065 DADVXU010000000
VERSION     DADVXU010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.068"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..1002
                     /locus_tag="KB953_001918"
     CDS             <1..1002
                     /locus_tag="KB953_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBB6866890.1"
                     /translation="TFSAKTDLVDEHHQLTKELKQAILMELYENQSRKLIAKKYFVSD
                     GTVTRILREATKHYQSRMNFLPTVLCMDEFKSMKSVSGSMSFICVDGTTHQLFTILED
                     RRLYKLTQYFLHFPRKARLKVKYLVMDMNASYCQLLKTVFPNAEIVTDRFHIVQHINR
                     SFNQLRVQIMNRFRTSHSENQKKYRRLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQ
                     TEIVDELLSYNEELRTAYHFCQLLRYYFVKRGTEGFQETLKTISSTLPQSFKKKFTIF
                     HCYQSGISNAFKVSHSNGVTEGLNNKIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            complement(1385..2113)
                     /locus_tag="KB953_001919"
     CDS             complement(1385..2113)
                     /locus_tag="KB953_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HBB6866891.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(2091..3665)
                     /locus_tag="KB953_001920"
     CDS             complement(2091..3665)
                     /locus_tag="KB953_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBB6866892.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            3894..4832
                     /locus_tag="KB953_001921"
     CDS             3894..4832
                     /locus_tag="KB953_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBB6866893.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            4844..5755
                     /locus_tag="KB953_001922"
     CDS             4844..5755
                     /locus_tag="KB953_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBB6866894.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            5779..7251
                     /locus_tag="KB953_001923"
     CDS             5779..7251
                     /locus_tag="KB953_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBB6866895.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            7302..7913
                     /locus_tag="KB953_001924"
     CDS             7302..7913
                     /locus_tag="KB953_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBB6866896.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            7943..9856
                     /locus_tag="KB953_001925"
     CDS             7943..9856
                     /locus_tag="KB953_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HBB6866897.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            9984..12965
                     /locus_tag="KB953_001926"
     CDS             9984..12965
                     /locus_tag="KB953_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HBB6866898.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            12962..14623
                     /locus_tag="KB953_001927"
     CDS             12962..14623
                     /locus_tag="KB953_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HBB6866899.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            14663..15595
                     /locus_tag="KB953_001928"
     CDS             14663..15595
                     /locus_tag="KB953_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HBB6866900.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         5158 a         2571 c         3187 g         4993 t
ORIGIN      
        1 accttttctg cgaaaacgga cttagttgat gaacatcatc aactaacaaa ggaactgaag
       61 caggcaattc tcatggaact gtacgaaaat caatcacgta aactaatcgc taagaagtac
      121 ttcgtctcgg atgggacggt gacacggatc ttgcgtgaag caacgaagca ttaccagtca
      181 cgtatgaact tccttccgac ggtgttgtgt atggatgagt tcaaatcaat gaagtctgtt
      241 tctggttcca tgagttttat ttgtgttgat gggacaaccc atcagctatt tacgatcctt
      301 gaagatcgca gactttacaa actaactcaa tacttcctgc actttcctag aaaagcccgt
      361 ttaaaggtca agtacctagt gatggacatg aatgccagct attgtcagtt gcttaaaaca
      421 gtctttccaa atgcggagat cgtaactgac cgctttcata ttgtccaaca catcaatcgc
      481 agcttcaatc aattacgtgt ccagatcatg aatcgttttc gaacctcaca ctctgaaaat
      541 cagaagaagt accgtcggct caagcgatat tggaaactct tactcaaaga ttccacgaca
      601 tgggagccac tcaaacggca ttatcatcga ctgtttaaac gaccaattag tcaaacagaa
      661 atcgttgatg agctgttaag ctacaacgaa gaattgcgaa ctgcgtatca tttttgtcaa
      721 ttattacgct attatttcgt taaacgaggt acggaaggct ttcaagaaac ccttaaaaca
      781 atttcatcta cgttacctca gtcgtttaaa aagaaattca cgatcttcca ttgctaccaa
      841 tcgggtattt caaatgcctt taaggtgtcc cactcaaacg gcgtcactga ggggctgaat
      901 aacaagatca agctgattaa acgaattgcg tttggttatc gcaactttta taactttcgg
      961 gcacggatct atttacaaca aggtctgatc tttgaaaact aaaaaaatat gcggcgcaca
     1021 gaacgcgccg catatcggac taagttttat tcaaatttgt cttcaccaac acgatttgac
     1081 gaagagccta tttaaataac tacatccatt tattctatct tctaaaatag cctttttcat
     1141 ttcagttaaa tagttcagcg ccatttttgc actcgacttg catagtagtt ttttccatat
     1201 tcctcaacct cagcgggggt tgaagacata aggaaaatcc tatgcgttat gctcaactgg
     1261 atctgattaa atattctaat gtatttttct ctaccatcat taaatggtta atagccaaaa
     1321 atcaccactt ttcaatggct ccttgttgta taagtataac tagaagatga acacgtctga
     1381 gaaattatag aagcttttta cggtaattac tggggctgtg tccagtagtt tttttgaaga
     1441 gaacagaaaa atagctcacg tgatctattc ctactttttg actaatttca tcaatagata
     1501 agaatgtgtc tttcagtaga gcacttgctc ttttgattcg aaagtctagc aagtaatcat
     1561 gaaaggaata acctgtttct tttttgaagg cccgtcctaa ataagcggga tggtaatgga
     1621 attgtgtagc tacatctttg agcgtaagct tttcactata attgttttga acgtaatcaa
     1681 tcacttgttg aattgactca ttaaaagaat catcagttag agagttttga ttagctgctg
     1741 atttacgcat tttttcttct tgtactaact taactaaata aggaaaatca atgggtttcg
     1801 ttatgtaact tttaatagat aaatcaatcc ccttttttgc atactgaaaa ttttggtggg
     1861 cactaatgac aatactagta aagtagcgat tggatgtttg ttgcaaagat tcgattaatt
     1921 cgagtccaga aattttcccc atcgtaatat ctgtgataag taaatcaaag cgctggtttt
     1981 gcaggttttt aaatgcttcg tctccgtctg tgtagcagtg atcgataatg atgtcgggga
     2041 ctttcttcaa aagataatgc cttatgcctt ctacaattgt acaatcatcg tcaacaattg
     2101 caattctcaa catgcatatt cccccctttt tgtattttga tagtaatcgt tgttccgacg
     2161 tgtagttggc tttctataac aaaacgtgta tctccaggat agagatgctt tatacgtcga
     2221 aaagaatttg agatgccgat attctcacta taaggaatat catcattaag tttttgttga
     2281 atatgctcca gcttgtcttg gtccattcct ttcccatttt caacgatttt taacacataa
     2341 tggttggttg tttcagttgc atagacagtc aaacgatttt ccttttttga tgaatcgaag
     2401 ccgtgtttaa tataattttc aacaaatggc tgtaaagaaa gttttggtaa cattaattga
     2461 tcaatatgat caatcagctg atagtcaaag ttatgtcgat aacgacgttg atgtagttgc
     2521 aaggagaggt cgatattaat taattcctct ttcaaatcaa ttatgtcggg ctgatatgta
     2581 ttattgcgat acaacttgct caataaatat atactgtcgc tgatttcttc gtcatcattc
     2641 aatatggcag tcattcgaat aaattccaaa ttattaaata gaaaatgagg ctcgatttga
     2701 tattgaagtg ctgcatactt tgcttcttgc ttcaaccaat tattttcata aagtgttttt
     2761 tgatatgtag cattatctaa ataaaactga ttgatatgca tagcgatttc tttcaaatca
     2821 ccgactttgt tttcgatatt gattgacttg tagtctcctg tagatgaatg atttaaggaa
     2881 gtaatgattt cctgatactg ctggatatag ttacgaaaac ttctatttaa taattgatga
     2941 atagcaagaa taaacaaact cattaataag aaaatcatga ttgccagacc taataaggtt
     3001 tgtgacgaat aatttggatt ttcatatact tgtaatgtgt ggttaccgat agctatctgt
     3061 tgcgtttttt tccatttaga aggcgagatg tcttcagata aattgacgga atcaatacca
     3121 ttagacaaag aataattgta atgtgttgga aagagactct tttttagcgc gttgatcgaa
     3181 acgtctagaa tcaatgtagc atcgggcatg tcctcactaa tgaaacttaa tgaacgaata
     3241 aaagaaattt tttcgttttt tgtaggtgcg taggcactta agtatttttc gtaatctaat
     3301 gagatagata tatttttaac ttcatcatct tgcacaagtt tttttaagtt atctactaaa
     3361 taggtagggg agcgatcctt tgacaaaata tcttccatgt attcagcagg tgatgagagg
     3421 tagtagtcat taaggttttc ttggaaatca taattttgat agatctctgt cacgataccg
     3481 gagagaatac gattatatgt ttccatatag tgggtgattt gctgaatgtt ttgcaggttt
     3541 tgattttttt ctttttcaat cacattgata taggaaaaga acaatgtgaa acaggcaagt
     3601 aaagtaataa aaaaaatcaa tatgtatgta aaacgcataa taacttttga taaatcgttt
     3661 ttcattcaaa gtcctcattt tgataatctc taattttatg aatcattata gcacatatta
     3721 caagtgatgt aaacgctttg caaacagggc tacttatgat tgtgaataca gtttccgttt
     3781 aaaaactatt ctattgtaga ggtttaataa actaaaaata atgtataaaa aattaatgta
     3841 agcgattaac caagtgggta taatcaagga aacgctgaga gaggagagac aaaatgaagc
     3901 taaagaaagg gttattagga aatctaaaaa aaaaccgagc attgtttatt atgcttttac
     3961 caggtgcgtt atggttattt atattttgct atataccaat gtttggagtt gtaatcgctt
     4021 ttaaagattt cagaatttca tcaaggggat tctttacaag tttgttcaac agtccttggg
     4081 ttggctggga aaatttcaaa tatctctttg cttcgcaaga tgcttggatc attactcgca
     4141 atacggttgt ttataacgtt gtaggtattt tgctaagtac tttttgtaca gtgtcattag
     4201 caatcattct gagccaaatg gcgaacaaac ggttattgaa aaagatgcag acatttatga
     4261 tttttcctaa ttttttatca tgggtagtca tcagttactt tgtttacgca tttttgagta
     4321 cggataaagg attaattaat attttactaa gcaattttgg tgtggaaccc ataaattggt
     4381 atagtgaacc gaagtactgg ccttttatta ttgtttttac gagtttatgg aaaggcgtag
     4441 gctatggatc catcatttat tacgcgtcaa ttatggggat cgatactagt tattatgaag
     4501 ctgctttagt tgatggtgcc agtaaatggc aacagataag gaatgtagtc ttgccacatt
     4561 taagatcgat tattgtcatt atgttgattt tgtctgttgg tagtattttt cgagctgatt
     4621 ttggattgtt ttatcaattg ccaagaaatt cgggggctct ttatgatgtg acgaatgtat
     4681 tagatactta tgtctaccgt ggattaatga atagcggaga tatcggaatg tctacggcag
     4741 cgggattata tcagtcatca gttggctttg tcttgatttt gattactaat aagatagtca
     4801 acaagttaga tcctagttca gcactatttt aggaggaatc aaaatgatat caaaattaaa
     4861 acggcagatg tacttttcgc tgccagtcga acggtttttc acaatattac taatcttatt
     4921 tactcttagc tgtatttttc cctttatttt cgtaatcgtc atttcattaa cagatgaaaa
     4981 agcattacaa ctcaacggtt acgaattatt gccggctcaa tggagcactg atgcatatca
     5041 atacttaata caagatggcg gacagttact tcggtcatta ggcgttacaa ttatgattac
     5101 agtgattgga acgttgatta ctgtttttat gacgggaacc tatgcttatg ttttatcaag
     5161 agcctctttc ccttaccgga agttctttac tttttatctt ttttttacca tgttatttgc
     5221 gggagggatg gttccttctt acttagtgat gacgaagatg cttggcttaa aaaatacaat
     5281 ttgggctttg attctacctc ttgcttttag cccatacaat gtcattattt taaggacatt
     5341 ctttaagaag tcgattccag aatcgattat cgaatccgct aaaatggatg gctgtagtga
     5401 gtttcgagtt tttttccaaa tcgttttgcc tttagcgatc ccaggggttg caacaatagg
     5461 tttattttca agcttaggtt attggaatga ctggttcaac gcattgctgt atattgacac
     5521 caacaaattg attccattgc aatatttgtt aatgaagatt caaaattcaa tggaattctt
     5581 agcaaataat aatgacatta cattagcgca gcaacaagca attcaaaata gccttcctca
     5641 agaatcaaca cgaatggcaa tggtcgtagt agctacttta ccgattgcaa ttgtttatcc
     5701 gttcttccaa aaatactttg ttcaaggatt gacgatcggt ggagtgaaag aataaaaaag
     5761 aataggagat gttaaaagat gaaaaagaac atgcagaaat atttgattgg tggtgcttct
     5821 gtactaaccc ttggcctatt aggtgcttgt ggtagcagta atggcgaagc aaataatgaa
     5881 acaggaacca caaaagttac ttggtatcag gttggagaag aacccaaaga ttttgatgag
     5941 gtcatgacaa aagtcaacga aaaattaaag gaagaaatca atgtagagtt ggatatgcgg
     6001 ctgattccgg acggggatta tcagcaaaag cttggtgtga tgatcaattc aggagaagaa
     6061 tatgatattt gtttcgtcaa tggcacagat tatgtgaatt atgggaacaa aggtgctttc
     6121 attagtctca acgatcattt ggataaagag ttgaaagatt atgcagctga attgaatgaa
     6181 ggatttattg aaggtggggc aattaatggc gaaacttatg gattgccagt caataacgat
     6241 attgccacaa cggaatattg gacctttaat aaagatttgg tagatcaata ccaattggat
     6301 gtagagaatg ttgaaggtgt agaaacattg gctccagtat tagaaaagtt tgcagaaaaa
     6361 aatactgata cgaatattac gccaatcggt atgacccgta ccagtgtctg tattccttat
     6421 gacaatatta tcggtggtgg tgtaccgcta gcggttgcat ttgaaggcga tacacaaaca
     6481 gttgttaata cttatgagac tccagaaatg atgacttatt tgaaggctat gcatgagttt
     6541 tataaaaaag gatatatttt gaaagatgca gccactgtta cgacagatcc atttcgaatt
     6601 gatagtaaaa actggttttt aggtcgtaac tcaagtggtt atcaaccatt agcacaaaaa
     6661 aaattagaag ctctagcggg acatggatta attttaaaac cagctggaaa agcttttaaa
     6721 acgactggat ttgcaagagt ggcaatgcat gcggtatctg cttcaagtaa acatccggaa
     6781 gcagccttga aattgttgaa ttatgtgaat accaatcaag aagtagcgga tttaatggtt
     6841 tatggaattg aaggaaaaca ctatgatcgc aatgaagatg gttcgattac tttaacaaac
     6901 tctgatgatt attatcaagg cgcttgggca ttggtaaata cttatctagt ttcaaaaact
     6961 tcaccgatta cagaagaaag taaaaaacta gaagaagaaa gtaaaaagtt tcaaaatgaa
     7021 gcaccgatgt ctcctatttt aggtttctct tttgacgctt ctgattttcg aacagaaatt
     7081 gcaagtgtac aaaatatcgt gtcacaatat gaagtacttt taagctcggg aacagttgac
     7141 cctgaacaat atgtaccaga gttgttagaa aaactagaaa aagctggatt ggataaaatt
     7201 caggcatctg ctcaagaaca atacgatgaa tggcgaaaat ctaacaaata attttttact
     7261 aaatgaaaag aatagactaa gcagttagtg gggggattca aatgaacgaa aacaaatgga
     7321 ccaaaggatt taattgggtt tttcaattgt tgttactaaa ctttacttat tttatactaa
     7381 tttgtttagg atttggaatt ttaggttttt tccctgctac gatagctttg attgctggcg
     7441 ttgtgaatcg aaaaaaatat ggcttgcaag agtctgtgaa gttcatgagt aaaatctata
     7501 agaaaaattt tgttagatat aatctatatt gctggtcagc aactttgttg ttggccggca
     7561 atattactac gatctggctt tgttggcagc aaagcgcacc attgttttat ttattaagta
     7621 cattattatc ggtaattaca ctgttcctag tctttgcaat gttgaattgg gtcgtgatca
     7681 aaagtcttgg gttgtggatg atgggagacc gactgattga ctatttgctt ttcacagcaa
     7741 cgatgttgcc aacggtagtc atgcaaggct tggtgcttgg aatttatttc cgaatattga
     7801 tgattttgcc tgtgattttt ctgtttggcg gaacagctgt aacggtaata ttgattagta
     7861 aattaactga aagagcgtta gcaaagttgc cttgtcagat acaatcgcac taattttttc
     7921 acaaaaagga gtatttcttt ctatgaagtt aactgataaa caagtagcta atatagcctt
     7981 tatccagcaa cttgagattc cattgacaca aaaagatgaa gaacaaatag acttgatttt
     8041 tactgacaca aaggctaatg tgcgacaaat caagattact aaaaatgagc agcaacgaat
     8101 ttgcttgtct tattcacatg aagacttgct gtatcgagga ttgtttatct gccttccacg
     8161 ttttttagct caaggaggag agatgccata cattgaagaa acctcgatca ttgagaccag
     8221 ttattcatta gatgcttctc gtaatggcgt gatgaataaa gagactctaa aaaaattcat
     8281 ttcttactta gcgttgatgg ggtacaacac attgtatatt tacacagaag atacctatga
     8341 agtagaagac cttccatatt ttggttacct tcgcggagcg tatacaaaag aagaagtgaa
     8401 tgaaattgtt gaatttgctt ctggttttgg catagaagta gttccatcga tccagaccct
     8461 tgctcacttg acccaatttt tgaagtggta tgctcaacaa gaactgatgg atgatcaaaa
     8521 tactctactg atcggtgaag aaaaggttta tgcggcaatc gaaaagatga ttattgcctg
     8581 caaagaaatg tatcagacag agcgtattca tttgggaatg gatgaagcct atcaagcagg
     8641 tctgggacga tatttaagcc tgtatgggta ttgtgatcgg acaactttaa tgattcaaca
     8701 tatggaccgt gtgttatcaa ttgttgataa atataagatg aaaccaatga tttggagtga
     8761 tttcatttat aagctattag atgaaaccgg taaagatagg tattacaatc ctgattttaa
     8821 actcaaagca ttagcagcta aaggattacc agcaggatta acctatgttc attgggacta
     8881 tggttcagat gaagtttcct cctatgagaa agtaattgat aaccatttga aattttgtga
     8941 cattgatcat tacgttttag ctagtggtgc ccatatctgg gggagactag ccccgaatca
     9001 tgggaaaaca gaagcaattg tttctgcagc tgtaaaagct tctaaaaata aaggtgtacg
     9061 ttctgtgatg ttaactactt ggggagatga agggcaggaa acagagcatt ggcattcctt
     9121 aattggggca ttaaaattag cagattatgt ctattctgaa gaagagtgga atgattttga
     9181 ggaaatctac gatcgtttat taggaacagg tagttatcaa ttaatgtccg aattacgata
     9241 tttcgatgaa gtggatacca ttacaaataa caacttacaa atggcttccg tgagtaaact
     9301 gctactttgg caagatccgt tagttggtat atatgacttt catgtagccg ctcataacaa
     9361 aattgccact agaacaattg gtacttatta tcatgagtta gctcagaaat tagatatgaa
     9421 taaaaattgg aatccaaaac aaccaatttt aacaactatt cgtcaacgat atatactttt
     9481 agctgaaatt ttagcacaaa aggcagaact aggcatctat ttacaacact gctgtctggt
     9541 agaagacctt gcaggattaa tggaattagc gaatcggtgt gaagcactag ccaatcgagt
     9601 aaatgaattg gcatctattc atgaaataat ttggtatcaa acatacaaaa cctatggttg
     9661 ggaagtatta gaacaacgat atgctgcatt agctcatcgt ctgaaaaccg ttgcgcgtcg
     9721 tattaaaaat catcttcagg ggcaagatgc cctagaggag cttttggaag aacgattacc
     9781 gttttgtcaa tatcaagtgc cgcttgaaat ttctggcttt aattatcggc aaagctcggt
     9841 gtcaggctac aattaacgaa ggaatcttcc cgttgaacgg accacttgct catctaatgg
     9901 gaaagtggtt cgttttctat tcataagtag cttaccacta actcttttat taagaagaga
     9961 aaaaaagtag gaagtgagaa aatatgttat cggactatta tcaaaatctg tcagttataa
    10021 gtgtcaatac agaaccatta cgtaattatt atattcctta tgcaagtaaa gaagaagcaa
    10081 aacaagagtt agaaagggaa aaaacgagcc agtttatcga tctcaatggt acatgggatt
    10141 ttcagtatta tccttctgtc caacagttac cggaaaattt ttttcaagag gcctttactg
    10201 aaagctatga tagaatttct gttcctggag tttggcaaac agagggattt gacttacctc
    10261 agtatatcaa tgtatcttat ccaattcctt ttgatccgcc ttatgtacct aatgataccc
    10321 cttgtggtgt atatcggcgt cagtttcaaa ttgagcaaac agatgcacgc ttttacttaa
    10381 atctagaagg agcagatagt aattgttttg tttggataaa tagaaacttt gtaggctatg
    10441 accaagtgag tcatagtaca tctgaatttg atgtgaccaa gtatttacag gttggggtta
    10501 atgaaattca aattctagtg atgaagtggt gtgatggtac ctactttgaa gatcaggata
    10561 agtggcgaat gagcggtcta tttcgtccag tgtatcttct tcaacgacca actatgcatc
    10621 tgacaagtta tcaactacgg acgttgactg atcctaaaaa aggaagcgga acattaatgg
    10681 tttcttttca acgatttact cctattgaag tatcgttacc tatttcttac gaattattcc
    10741 atcctgatgg acgtctcata gcgaaagggg agtgttactc ggaacaacaa ataaatttgg
    10801 atgttgaaga ttgccttttt tggacagcag aaacgcctaa attatataca ttacttttaa
    10861 catgtaatgg tgaatatttt aaagaaaaag ttggctttcg agcactgacg atcaagaatc
    10921 gtaagttatt gctgaacgga acacctatta aattatatgg agtcaactat catgacgtga
    10981 atcagcaagg cacgccagtg atgacccgag agcagttacg tgcagatttg ttattaatga
    11041 aaaaattaaa tgtgaatact gtacgaacgg cacattaccc taaatcacca gatttttatg
    11101 aattggcgga tgagattggt ctgtatgtaa tcaatgaagc agatttggag tgtcatggtg
    11161 tggttaatct atatgatact gaagcgaatt atcaactaat tgctaatgat ccgacattcg
    11221 aaaagccgat tatggaccga gtgatccgtt ctattcttcc ttatcaaaat cgaagctcta
    11281 tcattatgtg gtctatgggg aatgaatctg gttatggagt aaattttgaa aaagctcaag
    11341 ctttagcacg gaaattggat gattcacggt tgattcataa tgaacgctcg attgaaccag
    11401 ttattgacca agtgaatgat tttagtaact tggatgttat tagtcaaatg tatcccccac
    11461 tggaagatgt tcatcgtcat tgtcaaagaa gtgatactga aaaacctttt tttatgtgtg
    11521 aatatagcca cgctatgggc aacggtcctg gagatcttaa aacgtacttc gatttgattc
    11581 aacagtatga ttcctttctt ggcggttgtg tctgggagtg gactgatcat gcaatcaata
    11641 gaggaacttt agaaacgcca tcttatttat acggtggtga ttttggtgaa aaatatcacg
    11701 acggcaattt ttgcgtagat ggtttattga ccccagatcg gcaattaaca gtaggtgcat
    11761 tggagtaccg taatattcat tcgcctattc aactgattgc tgtagatgag gagctgcaag
    11821 aagtaacctt gagcaatata actgctttta cagccggttc agcatttaac ctttctcttg
    11881 aatggtatat agatggaaaa aaaacagttt atgaagtaat gaatcctttc atacttgagc
    11941 ctggcaaaag ttgttcattg cccatcacag tacctacaac gattcatggt gctcatacgg
    12001 ttgctgtttc gctatctgtt tataaaaatg atgagcttta tggacagcaa cagctctttc
    12061 ttaaaaaaga aaaaatgaga ttgccaagaa ctgctcatca taatgatgca ccacaaattt
    12121 cagtaaaagg cacagagctg ataatcaaag gagaatcctt tatttatata tatcaattag
    12181 caaaagctgc gttcacttct gtaaagatca atcaaaagga acttttggaa cgtcctactc
    12241 aattttcaat ttggcgtgca ccaacggata atgatcgtaa cttagtaaac aaatggaaag
    12301 cagctggata tgctgaaatg tctttagaaa tcagaaaaaa agactggaaa ttcgtagatg
    12361 atcatctcct gatcgaaacg tcttatcgag gagttattcc aggattacag ccaattttta
    12421 caatgcacat acagttgaaa ataaatggtg caggcgtaat caaccaaaaa gtatcagtgg
    12481 agaaaaatcc gatttttcca gatttccctc gctttgggat ggactggccg ttgaatgata
    12541 ctttttcgaa ggtttcttat tgtggctatg ggccgtatga aagttatcct gataagcact
    12601 atgctgcgat gtttggtatt tatgagatgg agattgagga atttgatcag ccatatatca
    12661 aaccacaaga atatggtaac cgctggcaat gtgattgggt atcaatcaaa aatcatcaag
    12721 gatctaccat gacggtttat ggaaactcta atttttctgc tctgccttat agtcaagctc
    12781 aattaaccga aaagtcacac cattatgaat taataaaaga aaatagaact tggttcaatt
    12841 taagttataa acaaaatggt atcggctcac acagttgcgg tccttattta gaagatcgtt
    12901 tccgttttat ggaagatact tttgactgga atatagtgat tactttcaaa ggagaaaata
    12961 gatgaaagaa acaaagatca atcaagtcaa aaaaaaatta tctcgttatc aagaagttgt
    13021 ttatcgaaac cattgcttaa aatggtcaaa aaataagctg aatgttcgtt ttaatggatt
    13081 attttatcgt ttcgaacagt tagaagaagt ctggaaaccg gtaacgaatt tccaatcatg
    13141 tcctgtacag ctcataaatg atccaaaaga aacatacgat ttatgtataa cacaccttta
    13201 taaccagcat ttgaaaaatg acggatttta tttaaaggtt actaatgaca atcaacttac
    13261 gattgaatca gcaaaccttc gagggtttca atatgctatg gaagccttat tagagagttt
    13321 tttttcggta ggagatcaat tattattacc ggaattgatt ctgaaacatg aaccatcagt
    13381 gaaaattcga ggaatcattg aagggtttta tggtatcccc tggacacatg aaatgcgcct
    13441 ctctcttttt tcatatatga aagataacca attgaatact tttatgtatg cccctaaaga
    13501 tgatgaatta ttacgtaaaa aatggcgtga actctacgat gcgcaagaat tagacaaatt
    13561 taaggagctt ttatctgctg ctgaggctag caatatcgat ttttggtact taattagtcc
    13621 tggaaatgat attgatatta cgtgtgaaga agacattcaa gttcttctga aaaagttaga
    13681 acaactgatt gagcttggcg tatttcaatt tggtctatta atggatgata ttgactatca
    13741 attaaaaggt gctgctaaac gccgttttcg agagccagca ttcgctcatg cctatttagt
    13801 gaatcgagta gaggaatatc ttagcactcg gttgtccaat catgaattag tcatttgtcc
    13861 tacagaatat gataatcgtc atggttctcg ctatttagca acgttaagtc aagaaatacc
    13921 agaacaatta ccgttctttt ggactggccc aagcacgtta gcggcgtcaa tttctacaga
    13981 agagctgcag gaaatggctt ctgtctatca acggccgatg ctgatttggg ataatattcc
    14041 ggtaaatgat tatttagagg ataaagaact attgttcatg agtccatatg agaatcgaac
    14101 accgaatctt tctaaggaga gataccaggt tacaggagta gtttcgaatc caatggctca
    14161 attagaagct tcaaagttta cgatcaatag tatggctaat tacttatgga actgtgagcg
    14221 ttttgatcct ttggaaactt ggacgtcagt cgttgaaaaa gtagttggaa gtaaactaca
    14281 accagcttat cttaccttaa ccaatgcttt tccaaatcat tacacaagca gtctgctttc
    14341 agatgaagaa ttactgctag ccaaagaccc agaatgggtc acaaaagaaa tggctgcttt
    14401 agtccaagca gtaaaatgga tccagcaaga acaacataca tctagattga gaacagaatt
    14461 ggagccttgg ttacaagcaa tcacagaaag ttggactttc tggcaagaat ggcaattaaa
    14521 aaaagatcca aaagaagcag aagaatggtt ggctaagaaa aaaacacacc gcataggacg
    14581 tgatttagta actgcccatt tagagcaaat tatcaaaagt tagatatctt aaaaaataaa
    14641 aagcaagaaa aaggagaaca aaatggtcaa gagattaatt agtgcaaact acagtgatgt
    14701 caagaaaatg acgtcaaaag agttgaagca atccatcaaa gcgagtgaag gacgggtgat
    14761 tgtttgtgaa acggttgcta atactcttcc acaatctgga gaaattacga atgcagaagt
    14821 tgcgtcagca tttggtgcag atttgattct tttaaatggt ttcgattgtt atcgtcctgc
    14881 agtttttgga ctaccaaatg tgacgattga gaaggttttt gatctacagc aaacgattga
    14941 aaacccgatt cctaaattaa aagaactagt cggtcgtcct gtgggaatta atttagaacc
    15001 cattgcccct aaaacaaata tgatgagtga gaaagtagaa atcagccaag gaagagcaag
    15061 ctctgttgaa acgatacaaa aagcagaaga actaggagtc gattttattt gctttaccgg
    15121 taatccaggc actggagtaa ccaatttaga aattactaaa tccgtaaaaa tggcaaaaaa
    15181 gtatttttcg ggattgatta ttgctgggaa aatgcatagc gccggctcag aagagccagt
    15241 tgtgaataaa gaggcgatta tttcttatgc ggaagcaggc gcagatattt tactagttcc
    15301 ttcagtaggg tctatcccag gctttttaga gcaagaccta attgaagcgg ttcggttggc
    15361 aaaaaaatat gatttgttaa cgatgtctgc aatcggtact agtcaggaaa gtgcccgacc
    15421 agaaacattg cggcaattag ccattatgaa taaaagttgt ggtgttgatt tacaacatat
    15481 tggagatgct ggatacgggg gattggcacc agcagaaaat attttcgaaa cctcggttgc
    15541 aattagagga atgcgacata ccatcaactg cgttagccgc tcgatcaatc gctaacaaaa
    15601 ttaaataaaa aatggttaaa ttgattattt aagtgggaac atacttgaga tatcagctaa
    15661 cttctgtcac tttaaaaaag ggactgtgac agaagtgaaa atagacaaaa aatatccgaa
    15721 caagagacta ggatagcaga aaaactatcg ttttgcaacg aggaccaact ggaccgtagg
    15781 agcaattctc ttattgttcg gatatttcgc ttgttcaagg acttatgtct gtagtgccct
    15841 tttttggtag gaaaaatggt agggaaccat atcattttta atgaaaatca agcatttcaa
    15901 aagttgaaa
//
LOCUS       DADVXU010000066        15650 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.069, whole genome shotgun sequence.
ACCESSION   DADVXU010000066 DADVXU010000000
VERSION     DADVXU010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.069"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(94..1137)
                     /locus_tag="KB953_001929"
     CDS             complement(94..1137)
                     /locus_tag="KB953_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HBB6866901.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(1514..2479)
                     /locus_tag="KB953_001930"
     CDS             complement(1514..2479)
                     /locus_tag="KB953_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HBB6866902.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            2713..3558
                     /locus_tag="KB953_001931"
     CDS             2713..3558
                     /locus_tag="KB953_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBB6866903.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            3749..4936
                     /locus_tag="KB953_001932"
     CDS             3749..4936
                     /locus_tag="KB953_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBB6866904.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            4942..5298
                     /locus_tag="KB953_001933"
     CDS             4942..5298
                     /locus_tag="KB953_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HBB6866905.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            5300..5638
                     /locus_tag="KB953_001934"
     CDS             5300..5638
                     /locus_tag="KB953_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HBB6866906.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    5714..5970
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6033..7067
                     /locus_tag="KB953_001935"
     CDS             6033..7067
                     /locus_tag="KB953_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBB6866907.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            7060..7746
                     /locus_tag="KB953_001936"
     CDS             7060..7746
                     /locus_tag="KB953_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6866908.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            7770..8618
                     /locus_tag="KB953_001937"
     CDS             7770..8618
                     /locus_tag="KB953_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBB6866909.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            8815..9588
                     /gene="sufC"
                     /locus_tag="KB953_001938"
     CDS             8815..9588
                     /gene="sufC"
                     /locus_tag="KB953_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HBB6866910.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            9601..10887
                     /gene="sufD"
                     /locus_tag="KB953_001939"
     CDS             9601..10887
                     /gene="sufD"
                     /locus_tag="KB953_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HBB6866911.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            10884..12119
                     /locus_tag="KB953_001940"
     CDS             10884..12119
                     /locus_tag="KB953_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBB6866912.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            12106..12576
                     /locus_tag="KB953_001941"
     CDS             12106..12576
                     /locus_tag="KB953_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HBB6866913.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            12581..13972
                     /gene="sufB"
                     /locus_tag="KB953_001942"
     CDS             12581..13972
                     /gene="sufB"
                     /locus_tag="KB953_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HBB6866914.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(14070..15212)
                     /locus_tag="KB953_001943"
     CDS             complement(14070..15212)
                     /locus_tag="KB953_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBB6866915.1"
                     /translation="MATFFSYKLQNGEKYWGFETYLGKDPKTGKDIRTKRRRFKSKAE
                     AQTALNRLLVDFKEKQEFKINVTTFQELYELWIDNYRTRVKPSSVAVAQRFCKNHILP
                     AFGELRLDKISVSYCQKQVNEWHKTLKQYGFLRKTTAQIFKFGVAMEVCKDNPMSKTL
                     LPRKIEEEQPLKFYTKDQLQLFLQNTKENNSVKLYTFFRLLAYTGMRKSEALALQWED
                     IDYFNKTITIGKTIAQDEFNQVVLQVPKTKNSSRTIQLDDFTLKQLRIWQQEQMKIMI
                     LYGYNTNSPKQFLFTTNTNKLYYPQVVNDWLDWIYKKTPMEPQITPHGFRHTHCSLLF
                     ESGASIKEVQERLGHKDIKTTMNIYAHVTPQSVKKTGDRFAKFMGE"
     gene            complement(15263..>15650)
                     /locus_tag="KB953_001944"
     CDS             complement(15263..>15650)
                     /locus_tag="KB953_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBB6866916.1"
                     /translation="IYLNEILANNINPLIEEYANQRHSISKELFFKRANSKLQDKIRN
                     GDFKYGEKERMIDSLKQLLDEMDLAYKDISKYSSVEDFFYKITKNQFETEKFISDLSK
                     KYNFPYEKNSIIAKFLTDNHDKFKNN"
BASE COUNT         5105 a         2896 c         3312 g         4337 t
ORIGIN      
        1 tgttgttgtt agaaggatat ctgcaaaatt aaaaacagtt gtgattcgga cattgttcca
       61 tgcgagaatc acaactgttt tttcatgttc attctaaact ttgatcagcc aaataaatag
      121 taaagccatc actgtttgga gaagcccgac agctaatcca taagataaga agcgtgtacc
      181 ttctttcatg aattttgcaa agtctaaacg cagaccaatt gccgctaaag cagtgatctc
      241 aaaccagctg ctgataaaat gtgccgattg gtcaataact acaggtaaag aaaacaagct
      301 gttgaccaaa caaaagatca caaaccctaa tacataccaa ggaagcggta attttcgttt
      361 tttctgcgta tcagttggcg tttcaaacag agctggttgt ttcttgtgca tgaatttttc
      421 aaacagatag acaacaacaa cgagcatcat aatacgtaga atcttaaata gcatggcgaa
      481 ttcgacaaca gaattgtcaa gtaagcttgc cccagcgact acttgaccaa cagattggag
      541 tgtaccaccc aaaagtgcac ttttttcaat gagatttcca ccaaacaact gtaatccaag
      601 aaaagggaga gtcaacatca tcacagtacc cagtagattg actagtgtga tgatctgtcc
      661 tttttcttcc tctttcgcat ggatggaagg ggcgattgaa gcaatagcag aagaaccaca
      721 aacagcgttt cctccagcca tcatcaaaga agaagtatcg ctgaattgca gttttttacc
      781 aatcaagtaa gcaccgatga tagtgactga catttgcagt aacacaaaaa caattccttt
      841 tacaccaatt tgagcaatcg tttgagctgt gacagttgca cctagtaaaa aaacagagaa
      901 ttctaatagt ttgctttcag caaattttgt gccgattgct agaacaggct gtttaaaaaa
      961 agtatttcct aataaaatac ccaataaaat agcaatagtc gctccaccta actgtggcag
     1021 ataaatagca agaagcctgc tgaaggcagc gatggcaaaa gaaagcagta aaccgggtaa
     1081 gatttcgcgg accgctgaat cctttacaga ttttattgga tgtgacaatg atttcatttg
     1141 atcggttcct ttcgtcaata aatactaaaa gcatactcga acatagtagc actaaaccag
     1201 tattttgaca aaagaacaag aggtcgtgac aaaagtcttg tacgacctct tgtttcgaat
     1261 aaacggtgtt ccagaagtag cttctacgga aataagccga aatttcacaa aaatttgaga
     1321 agcaattttc gtaaattcct tcttatttct tgaagctgac cacttctttt acgacctctt
     1381 ggtaaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaaccagta caaaaacatg
     1441 aacagctgtt tttgtttggc cgttcttatt acttgatgca gaacagcttt ttcacaacct
     1501 attgaatcga ttattaagcg atttttttag aaacaaattt tccatgtttg ctatgttctt
     1561 taattaagaa acgtacgtaa tgagcaagaa cgtagaagac aactacactt gcaaacagac
     1621 attccgcaac ttttaagaaa tccaatacgc tactatagat acctaaactg ttgaagtaat
     1681 gaacagtagt aaatatagct gttgcagaga taacaagcgg gaatgtaaag gctgcatagc
     1741 ttggataaaa acgaagcttg aacaaacgtg gaagcataga cagcacaacg aaatacaata
     1801 cttgagataa agcgaacaat gctacgacaa gataaaaatt aggttcagca aagtttttaa
     1861 gataacctgt taggcaaaga gaaccaggtg ctgcaacgat cgtaatcagc ggacgtgtcg
     1921 gatgagccat atttcttacg aaaaagacac gtttgatgat gatcggcaat aagaacgcat
     1981 agcagatcaa tgaaatccaa aagaaaattt ttccaataag tgggaagaag ttcccggcag
     2041 ttacagtgat gaccccaaat ccaacgtaaa tgataaacca gcttggataa attgcttcaa
     2101 ttgaaagatc agctttgata acatggtgat aattgaaata aaagacaagg atcacttgga
     2161 agataacagc gcacagccaa atgaatttga caattggtgc aatactttca tatgaaacaa
     2221 aataagtaca aataaccatg agtgacatcg taaaagttgg tgaaacggag gcgatgattg
     2281 gatcatgcaa cgtttgtttc gtgtgttcga ataagataaa caatttaaca aaaatgaata
     2341 tcataagaat catagagata ccgccagtta gattgcctaa agcaacatgg tgatagtctt
     2401 taaacagatt tccaagggaa gctaacccaa ggatcaaccc gcagatcggg atcggaataa
     2461 ttttcaaaaa gttcttcata tataatattc tcctttttat ttaatagatt ttagtaccaa
     2521 gatgaacgaa tacattgtaa aatgaatagg aatgtatgat tacgagcaag cagacagctg
     2581 attgtcttca tctaagttac tagttggatc agcaactgtt ttgttgtaca tttctattat
     2641 agatggtttt ttgtatattt aaatcaaata gtttttatag taattatcat aaaaagttat
     2701 ggaagtggtg ccatgtttaa gttattaacg acttttcgag cagtatatga aacaagaaat
     2761 ttttccagag cagcagaagt actgttttta tctcaaccag cagtctcgaa tcaaatcaaa
     2821 cagttggagc gggaattgaa tatcgatctt tttcaacgta acggaagaaa agagatgctt
     2881 ccaactaagc aagccgactt attatatgaa cggacactgg ttttgttaga agaatgggaa
     2941 gaaactcagc aaaaattgct caatgaacaa aatgaagaag aagtatgtcg gatcgttgct
     3001 tcccacacct ttgctgtcta tcttttgcct cagttgatga aacatctgat caaaaggttt
     3061 cctaaagtgc attttgagat cgaaatcacg aattctcatg aatcattaga acaagtagcg
     3121 aaacatgaat ccgattttgg ttttatcgaa aagccattag agacttccgg cgtacaaaga
     3181 tactctttga tggaagacca gctagtcatc gctggggacc caaaaagcaa attgtggctt
     3241 gtaagagaag caaactctgg tgtttttcat tacacgcagc gttattttga agagatgaat
     3301 atccaagata agaagatgat cgtgaaaagt aacgatgtaa ttgttgcgtt gctgaaggaa
     3361 ggaatcggtc gctcgatttt gtctaagcgc tctgttccag aaaatgtgcc aatgactccg
     3421 ttgaatttcc atcgatattt ttatttcatc caacgaacca atctggtttc tgatcagctg
     3481 ctagagattg ccgaagagat ccttcgatac aatgaaagca cagaaacttc tcacagcgag
     3541 gagaatctca gctggtagat aattgcatgt atcaaaaaaa gttctatgat acactaaaga
     3601 taagcagtag gcttttgtac tgcatttgct caagcaactg cacaaacgga aagttaattt
     3661 ttcccaaatg tctatggaaa gactttccat tatctaactg gaccgctata ataatcagca
     3721 gtatggtttt taagaaaagg gatgaaacat ggaaaaaaaa ttaaaagctt cgaacgataa
     3781 tcgtattgat tatggcgtga ttttaccagt ctttctgtta tgtttgattg gtctgctctc
     3841 attatatgta gcacttagcc atgatccgaa caatccagca gtcttgaaag gcgtagggat
     3901 gcaagttgtc tggtatttag ttggagcggc agcaatcgtt gtcatcatgc atttgaattc
     3961 caaatggatc tggaaactga ctccttacct ttatggtttg gggttagtgg taatgggact
     4021 cttgctcaaa ttctatgatc agaatttagc tgcttcaaca gggtctaaaa actggtttcg
     4081 attcggtgcg tttactttcc agccagcaga gttgatgaag attgcgtaca tcttgatgat
     4141 ggcactgatc gtaactaaac ataatacgca agtaaaagaa cggacgatga aatcggactt
     4201 ttggctgatt gggaagctct tgatcgtgac aataccggtt ttagcattga tcatggccca
     4261 agatgacttt ggaacgatgc ttgtctttct tgcgatcttt ggcgggattt tcctgatgtc
     4321 tggaatctct tggcgcatca tcgcacctgt agtaatcttg gcagtagtcg ttggagcagg
     4381 aacgatcttc ttggttacga ctgaaggcgg aagagacttg ctttataaag ttggattcaa
     4441 atcttatcag tttgcacgta ttgattcatg gctggaccct ttccatgata cgtcaggaat
     4501 gagttatcag ccggcccaag gacttttggc aattggtacc ggtggcttgt ttggtaaagg
     4561 attcaacgta agcaatatct atgtgcctgt tcgtgagtcc gatatgattt ttaccgttat
     4621 cggtgaaaac ttcggcttta tcggtggtgc attcgtcatt ttcttgtatt ttattttaat
     4681 ctatcgtatg attcgggtgt gtttcgatac gaataatgaa ttttacgcct atatcgcaag
     4741 cggtttgatc atgatgctgc tgttccatgt ctttgagaat attggagcaa atatcggatt
     4801 acttccttta acagggatcc cattgccttt cattagtcag ggaggatcct cgattttagg
     4861 gaacatgatc ggtatcgggt tgatattatc gatgagatac caaaatgaag cacctgcaag
     4921 atcttcaagg aggtaagaaa attgtacacc ttttactggt acccgaaatg ttcaacatgc
     4981 aaaaaagcca aagcgtggtt agaccagcat ggcgttagtt atcaaaccgt tgatatgatc
     5041 aaagaagtac cgaaggcctc aacgattgag acttggctag aaacgagtca agtaccaatc
     5101 cgtcgcctgt tcaacacaag cgggatgaaa tatcgggaac ttggactgaa agatcaagta
     5161 ggcgagttta cggcttcaaa agcgagccaa gtcttatcga cagatggtat gctgatcaag
     5221 cgaccactga tcttacgaga ggaccagctt gttgcaattg gattcaatga aaaaatttat
     5281 gaaggagaac tgatataaaa tggcaggcaa gcatttgaag aaaaaggaca acctttggat
     5341 cttatttaat ggaaaagaat actgtgttgg tttgacaaat gaagcgcaag aagaacttgg
     5401 agacatcacg tttgccaatc tgccaaaagc aggacaatcg ctaaaaaaag atgaacctct
     5461 gatcgaagtt gaagcggaaa aagcggtcaa tgaatttgcc agcccgctaa cgggtacagt
     5521 ttcttctgtt aatgataaaa tcggtgaaga tatcaatatc ttgaatgatt cagatgaaat
     5581 gaatgcgtgg atcttaagtt tcaaagatgt tgatccaaaa gaatttgacg aattataaaa
     5641 tcagaaaatc acttgaaaaa agttgacact gattcttcca gttgctataa tcataagcaa
     5701 tcctatatga aacgagaaag ctttgaaaag aagagtacat gtcacggacg tttgaagaga
     5761 gccctgtttg ctgaaaatgg gtaacgaaaa tgagatggaa gatggtctta gagcagagta
     5821 agcgagcgta agcaaactta ctacgggagt gcccgttaca gcgcgacagt atacggaaag
     5881 aagctaagtc caagtactgg aagaggctgt tattgcgaag taacggtaaa tcaaggtggt
     5941 aacacggaag ttcaagcttt cgtccttttg cttaaggcaa gagggcgaag gcttttttgt
     6001 ttgaaaaatt ataagagaaa gatgtgaatg gcatgtcatt aatccaacta acgaatgtca
     6061 aaaagacatt caatgggaaa aacggaaaca tccaagccgt caatgacgtt tcactaaacg
     6121 ttgaaaaagg ggatatttat ggaatcgtcg gctattctgg cgcaggaaaa agtacattag
     6181 tccgtctcct gaatggactt gagcttccca cgagcggcga agtgatcgta aacgatcaag
     6241 atattacaaa attaaaaaac aaagaattac gtacattccg aaagaaaatc ggtatgatct
     6301 tccagcattt caacctgtta tggtcaagaa ctgtacttga aaatatccag ctgccgctgg
     6361 aattagctgg cgtaccaaag agcaaacgta aagaacgggc agaagaactg ttgcgtttag
     6421 tcgggttaga aggacgcgga caagcatatc cttcacaact ttccggcgga cagaaacaac
     6481 gcgtcggtat cgcacgagca ttggcaaatg atcctgagat cctgctttgt gatgaggcta
     6541 ctagcgcatt ggacccgcaa acaacagaag aagtattaga cttattgtta gcgatcaata
     6601 agaaattgaa tttgaccatc gtcttgatca cacatgaaat gaatgtgatc cgcaaaatct
     6661 gcaacaaagt cgctgtgatg gagttgggga aagtcgtaga agaaggagac gtattgactg
     6721 tcttcagaca accgaaaaaa gaagtaacca aacgctttgt acaacaagac atcgaaccag
     6781 aagctgattc gacagaacta ttagctgaac tcatcaaaga aaatccaagc ggccgtttgg
     6841 caactttgac ctttagcgaa agaaatgcca acgagccagt catttcccaa gcgattcgac
     6901 gctataacat cgatgtcaac gttgtgtatg gaaagatcaa acaagtgaaa gaaggttcct
     6961 ttggttcatt gactgtcttg atgactggtg aaaccaaaga attagatcaa gcagaagtgt
     7021 tcatcaaaga acaaggagta ggaattgagg tgatccatcg tggataaaag cttttcagaa
     7081 acatatctag attttaaaca aatcaacccg gaaacgatga atcaggctat ccttgataca
     7141 ctatatatga cattaatctc aatggtctta gtgacgatca tcggattagt tttgggattg
     7201 atcctttact ctattggacg caaaaacaaa ccgtatgctc gaatcattta tggaatcgta
     7261 tcgatcatca gtaatatttt ccggtcaacg ccatttatga tcctgatggt tttgatcatt
     7321 ccatttacta aagcaatcgt aggcactatg ctaggtgcgc aagcagccat tccagctctt
     7381 gttttatccg cagcaccatt ttatgcccgt ctagtagaga ttgcttttcg tgaagtcagc
     7441 cctggtgtac tggaagcagc agatgcaatg ggagcaagtt actgggaaat catctggaag
     7501 gtcttgatac ctgaaagtat tcccgcattg atttctggat taacagttac aacgatctcg
     7561 atgatcggat ttactgcgat ggccggcgcg atcggcgcag gtggacttgg aggtcttgct
     7621 tggcaggaag gctaccagcg tggtaatcta acagtgacat tcgttgcgac tttaattatt
     7681 ttactaatcg tatttatcgt tcaaggaatc ggtgatttct taactaagaa aacagacaaa
     7741 agataatagg atataggggg atgtaggata tgaaaaagaa aatttttgga tttgcagcag
     7801 cgcttttgct gactgtagga ttagcagcat gcggtaacaa cagcgacagc aaagattcag
     7861 caaataaagc agacgacacg acattgaaag tcggcgcttc accgacaccg cacgcagaga
     7921 ttttggaaca tgtgaaacca ttattgaaag atgaaggaat cgatcttgaa atcgtcaaat
     7981 tcgatgacta tgtattaccg aaccaatctt tggaagaagg cgacattgat gccaattact
     8041 tccagcatat tccttatttg aacaaagaaa tcaaagaaaa aggatatgac tttgtcaatg
     8101 ccggtgctgt ccacatcgaa ccaatgggct tgtactctaa aaaagtcaaa gatgtttccg
     8161 aattaaaaga tggtgcaaca atcattactt caaattctga atcagactgg ggacgcatca
     8221 tcacaattct acaagatgct ggcttagtca aagtcaaaga tggagtagat ctggaaacag
     8281 caacattcga tgatattgaa gaaaacccta agaacttgaa gttcaaccat acgatcgacc
     8341 catcactttt agcttctacg taccaaaatg atgaaggtga cctagttgca atcaacgcaa
     8401 acttcgctta cggcgcaggg ttgaatccat tagaagatgc cgtattgctt gaaaaggaca
     8461 actctcctta cgtgaatatt attgcgactc gtaaaggtga tgaagacagt gacaaaatca
     8521 aaaaactgat tgaagtgctt caccgcaaag aagtccaaga atggatcaca gacaaatggg
     8581 gcggctctgt taaaccagta aaggctgatg caaaataagg tatggaacaa aaaagatgag
     8641 aaataagtct gtaatggctt atttctcatc ttttttatac ctattctaat ttagaaatca
     8701 aaattgttta ataataaata aaagatattt gattttagta aggaaggtca ttgcgcttca
     8761 ttctcaatta gtctaaaatg agtgagaacg aaagagaatt tgggggtata atgcatgtct
     8821 gtcttagaaa taaaaaatct acatgtgtcg atcgaggaaa aagaaatcct taaaggcgta
     8881 aacctaacaa tgaaaaccgg agaaatccat gccatcatgg gacctaacgg cacaggaaaa
     8941 tctaccttat ctgccgctat catgggcaat ccaaactatg aagtaacaga aggcgaaatt
     9001 ttgtttgacg ggaaaaatgt tttagacttg gaagtagatg aacgtgcgcg acttggctta
     9061 tttttagcta tgcaatatcc aagtgagata ccaggtatca cgaatgcgga attcatgcgt
     9121 gcagcgatca atgcgaaacg tgatgaaaac gacaaaattt ctgttatggc atttttgaaa
     9181 aagttagatg aaaaaatggc tttactaaat atgccagaag aaatggctga acgttatctg
     9241 aacgaaggtt tttctggcgg agaaaagaaa cggaatgaga tcctgcaatt gttgatgtta
     9301 gaacctacat ttgcaatttt agacgaaatc gattcaggtc tggatatcga tgctttgaaa
     9361 gtcgttgcta aaggagtaaa tgaaatgcgc ggagacaatt tcggtgcatt gatcatcacc
     9421 cactatcaac gattattgaa ctatatcaca cctgatgtcg ttcacatcat gatggaaggc
     9481 cgagtagtca tgacagggaa tgctgaccta gctaaacgtc tggaagcaga aggctacgct
     9541 ggtatcagta aagaattagg aatcgactat aaagaagaag aagcgtaagg gggagcaaga
     9601 atgaaagaaa aaaactggct cgattatctt gacgcggtaa atgatttttc tttaagtaaa
     9661 ggcgaacctg attggatgag aaccttccgt caagacgcac tagcgaaagc agatgaactg
     9721 ccattgccgc atatcgatcg ggtgaaattc catcgctggt cgttatttga tgtgaaagaa
     9781 acacaaacca tcagtgaaac aggcacgatt ccggcatttg atgcaatgaa agacaatcct
     9841 gtgcttgtcc aacaaggttc atggacgatt tttgaacaac tgcctgtgga attagcagaa
     9901 aaaggagtca tttttaccga cttgttcact gcaatgatcg aatatccaga actagtccaa
     9961 gaatattata tgaaaaaagc agtaaacatg aacgaagacc aattgactgc actccatgta
    10021 gcttttatga acagcggtat tttcctgtat gtgcctaaaa atgtcgtgat cgatgaacca
    10081 ctcgagtcct tattcattca agatggcgca tccgatgagc acttcttcaa gcatgtgttg
    10141 atcgtggcag atgaacatag tgaattttct tatctggaac gtttccagac aaccaaagaa
    10201 caagtagcaa aatcatcagg taatatcatc gtggaagtca tcgctaaagc tggttcaaaa
    10261 atcaaatatt cagccgtaga tcaattagga gaaaatatta cttcttatat gaatcgccgt
    10321 gggcacattt tacgagatgc ttctgtcgac tgggcaattg gtgtcatgaa tgacggtcat
    10381 gtgatcgctg actttgactc agatttagca ggagaaggtg cacacgcaga agtcaaaatc
    10441 gtggcaatca gctcgggcag acaaatccaa ggaatcgata cacgagtaac caataaagcc
    10501 cctcatacga tcgggcatat tttgcaacac ggagtgatcc gagaaaaagg aacattgacc
    10561 tttaatggta tcggccacat cttgaaaggg gcaaaaggtg cagatgccca acaagaaagt
    10621 cgtgtcttga tgctttctga taaagcacgt ggtgatgcga atcctattct tttgatcgat
    10681 gaaaacgaag taactgctgg acatgctgcc agtgtaggac gagttgatcc agaagaaatg
    10741 tactatctga tgagtagagg attgcataaa gaagaagcag agcgcttagt gattcgagga
    10801 ttcctcggtt ctgtcttaac agcaatccct gtagaacaag ttcggaaaga attagtcgaa
    10861 gtaatcgaag ggaagttgaa tggatgatcg atgctaaaaa gctacgcaat gattttccta
    10921 tcttatttca agacgtcaat gatgaacctt tagtttatct ggataatgca gcgacgaccc
    10981 aaaagccaaa acaagtactc caggcattag cgtactatta cgaacacgat aacgccaatg
    11041 ttcatcgcgg tgtccatacg ttagcagaac gcgcgaccca tgattatgaa caagcaagag
    11101 aaaaagtacg ggcgttcatc catgcaaaag aaacagcaga agtgctgttt actcgtggta
    11161 cgacgacaag cttgaattgg gtagccagaa ccttcggcga ccgctatatc aaagcaggag
    11221 acgagatagt gctctcttac atggaacatc attctaatgt tatcccgtgg cagcagttgg
    11281 caaaacgaac aggtgctgtc ttgaaatata tcgaactaac agaagaaggc ttcctggata
    11341 tggaggatgc agcgaaaaag attacggaca aaaccaaaat cgtctccatt gctcatgtat
    11401 ccaatgtctt aggtgtcttc aatcctgttc aagaattagc tgaacttgtc catgaaaacg
    11461 gcggggtcat cgtagtagat ggtgctcaat ctgccccgca catggccatt gatgtccaag
    11521 cactagactg tgatttcttt gctttcagcg gacacaaaat gtgcggacca acaggtattg
    11581 gtgttttata tggtaaacga aaatggttag aagaaatgga acctgttgaa tttggtggcg
    11641 aaatgatcga ttttgtcgaa ttatacgaca gtacatggaa agaactacct tggaaattcg
    11701 aagcagggac accaaatatc gccggcgcta tcgcattagg ccatgcaatc gattatttgc
    11761 aaaaactcgg tatggacaat atccatcgat atgaagaaga actagccgct tatgtgttgc
    11821 caaaattaca agcaattgat ggattgactg tttatggtcc acaagacccg aattttcgga
    11881 caggagtcgt tgcattcaat ttggatgggt tacatcccca tgatgtcgcg acagctttag
    11941 acatggaagg ggtagccgtc agagcaggtc atcattgtgc gcaaccgcta ttgaagtacc
    12001 ttgaagtatc agcgactgct cgcgcgagct tttatttcta caacacaacg caagacgccg
    12061 accgtttagt tgaagcaatc atggcaacaa aggagttttt ccaacatggg actatctaaa
    12121 ttagacaatt tataccgtca agtgatcttg gatcattctt cccatccgca tcatcgtgga
    12181 agcttgactg actcgacaag caagatcgaa ttgaacaacc ccacatgtgg agatgtgatc
    12241 gaattgcaag tcgatgtaaa agacggaatc atcaaaaata tcgcctttga cggcagtggc
    12301 tgctccatca gtacggcaag tgcctcgatg atgacagacg ccgtcatcgg aaaatcaata
    12361 gaagaagcag aacatttaac tgtagacttt ctacagcttg tccaaggaaa agaagtcaaa
    12421 gatcccgacc aattaggcga tgctgctatg cttggtggtg tagctaaatt tcccgctcgg
    12481 atcaaatgtg cgacacttgc ctggaaagca ctaggaaaag caatggaaaa cgatggtacc
    12541 gcagctttcc atcattacca caaggaagag gagtgagaac atgggcgtac cagaattaga
    12601 agaatataaa ttcgggtttc acgatgatgt cgaaccggtt ttcagtacag gagaaggctt
    12661 gacagaagaa gtgatccgtg agatgtcaag aatcaaaggc gaaccagaat ggatgcttga
    12721 attccgtctg aaatctctag aaacatttaa caaaatgcca atgcaaacat ggggccccga
    12781 cctttcagac atcgatttcg atgccatcaa atattaccaa aaaccaagtg acaaacccgc
    12841 tcgtgactgg gaagacgtac cagaaaaaat caaagataca tttgaaaaga ttggtatacc
    12901 agaagctgaa cgagcttatc tagccggtgc ttccgcacaa tatgaatcag aagtcgtgta
    12961 ccataatatg aaagatgaat tcgaaaagtt aggaatcgtc tttactgaca ctgactctgc
    13021 tttgaaagaa taccctgaat tattcaaaga gtacttttca aaacttgtac cgccaaccga
    13081 caacaaatta gcagcgctga attcagccgt atggtcaggt gggaccttta tttacgtgcc
    13141 aaaaggcgtc aaagtcgatg ttccattaca aacgtacttc cggatcaatg cggaaaacac
    13201 ggggcaattc gaacgtacgc tgatcattgt tgacgaagga gcaagcatcc actatgtcga
    13261 aggttgtacg gcacctactt attcaagtaa cagcctgcat gccgccatcg tagaaatctt
    13321 tacaagaaaa gatgcgtatt gccgttacac aacgatccaa aactggtcag acaatgtgta
    13381 caacttagta accaaacgag caaaagcata cgaaggcgca accgttgaat ggatcgacgg
    13441 aaacctaggt gcaaaaacaa cgatgaaata cccaagtgtt tacttagatg gaaaaggtgc
    13501 acgaggcacc atgctctcca tcgcctttgc aggagcaaac caaatccaag atacgggtgc
    13561 aaaaatgatc cacaatgcac caaatacatc tagctctatc gtctctaaat caatttcaaa
    13621 agacggcgga gaagtaaact atcgaggaca agtaaccttt gccaaaaaca gtgcgggttc
    13681 gatctctcat atcgaatgtg acacgatcat catggacgaa tggtcgaaat ccgatacgat
    13741 cccattcaat gagatccaca acagccaagt ctctttagaa cacgaagcca aagtctcaaa
    13801 aatctcagaa gaacaactct actatctgat gagccgaggc ctgaccgaat ctgaagcaac
    13861 tgaaatgatc gtcatgggct ttgttgaacc attcaccaaa gaacttccaa tggaatatgc
    13921 agtcgaactc aatcgattga tcagttatga gatggaaggg tctgtaggtt aataactatt
    13981 tcattgctcg aaacgttgat attaaagcgt ttcgagcgtt tttttatttg tattttaatg
    14041 gttggctacg tttttggcta cgtttggaat tattcgccca taaattttgc aaatcggtca
    14101 ccagttttct taacagattg tggtgtgaca tgagcataga tattcatggt agttttaata
    14161 tctttatgac ctaatcgttc ctgtacttct tttatcgacg caccactttc aaacaataag
    14221 ctacagtggg tatgcctaaa tccatgtgga gtaatttgag gctccatcgg tgtctttttg
    14281 taaatccagt ctaaccaatc gtttactact tgggggtagt acaacttgtt tgtgtttgta
    14341 gtgaaaagaa actgttttgg tgaattcgtg ttatagccat ataagatcat tattttcatt
    14401 tgttcttgtt gccaaatacg cagttgttta agtgtaaaat catcaagctg aatcgttctt
    14461 gatgaatttt tagttttagg tacttgtaga actacttgat taaactcgtc ttgagcgata
    14521 gttttaccaa ttgttattgt tttattgaaa tagtctatat cttcccattg aagcgccaat
    14581 gcttcgcttt tacgcattcc tgtataagct aataaacgga aaaaagtgta cagtttaact
    14641 gaattatttt ctttagtatt ctgtaaaaac agttgtaatt gatctttcgt gtaaaacttc
    14701 aagggttgtt cttcttcaat ttttcttgga agcaatgttt tagacatagg attatcctta
    14761 cagacttcca ttgctactcc aaatttaaat atttgagcag ttgtttttct caagaaacca
    14821 tattgtttta atgttttgtg ccattcattc acctgttttt ggcaatagct aacacttatt
    14881 ttatccaatc gtaattcccc aaaagcaggt aatatatggt ttttgcaaaa acgttgagca
    14941 acggcaacag aagacggttt tactcttgtt ctgtagttat caatccataa ttcatacaac
    15001 tcttgaaaag ttgtaacatt gattttaaac tcttgttttt ctttaaagtc tacaagtagt
    15061 ctattaagtg ctgtttgcgc ctcagctttt gatttgaatc ttctacgctt tgtacgtata
    15121 tcttttccag tcttgggatc tttccctaaa taagtttcaa atccccaata tttttcgcca
    15181 ttttgtaatt tataactaaa aaatgttgcc atttgattct ctccttactt cattattgca
    15241 ggtagagaag ttatttgaag aatcaattat ttttaaattt atcgtgatta tcagttaaaa
    15301 attttgcgat tatactattt ttctcatatg ggaaattgta ttttttacta agatctgaaa
    15361 taaatttttc tgtttcaaat tggtttttag taattttata aaagaaatcc tcgacagatg
    15421 aatactttga aatatcttta taggctaagt ccatttcgtc taatagctgt tttaaagaat
    15481 caatcattct ttctttttca ccgtatttga aatcaccatt tcttatcttg tcttgaagtt
    15541 tactgttggc tcttttgaaa aatagctctt tagagataga gtgtctttgg ttagcgtact
    15601 cttcaatcag aggattgata ttattagcaa gtatctcatt tagataaatt
//
LOCUS       DADVXU010000067        15226 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.070, whole genome shotgun sequence.
ACCESSION   DADVXU010000067 DADVXU010000000
VERSION     DADVXU010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.070"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            278..787
                     /locus_tag="KB953_001945"
     CDS             278..787
                     /locus_tag="KB953_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBB6866917.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="KB953_001946"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="KB953_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBB6866918.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="KB953_001947"
     CDS             2322..3302
                     /locus_tag="KB953_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBB6866919.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="KB953_001948"
     CDS             3424..4842
                     /locus_tag="KB953_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HBB6866920.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="KB953_001949"
     CDS             4913..6382
                     /locus_tag="KB953_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBB6866921.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="KB953_001950"
     CDS             6392..6859
                     /locus_tag="KB953_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBB6866922.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="KB953_001951"
     CDS             6895..7767
                     /locus_tag="KB953_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBB6866923.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFIKSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7848..10382
                     /locus_tag="KB953_001952"
     CDS             7848..10382
                     /locus_tag="KB953_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBB6866924.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10538..11311
                     /locus_tag="KB953_001953"
     CDS             10538..11311
                     /locus_tag="KB953_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBB6866925.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11304..12095
                     /locus_tag="KB953_001954"
     CDS             11304..12095
                     /locus_tag="KB953_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBB6866926.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12105..12596
                     /locus_tag="KB953_001955"
     CDS             12105..12596
                     /locus_tag="KB953_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBB6866927.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12614..13024
                     /locus_tag="KB953_001956"
     CDS             12614..13024
                     /locus_tag="KB953_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBB6866928.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13028..14428
                     /locus_tag="KB953_001957"
     CDS             13028..14428
                     /locus_tag="KB953_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HBB6866929.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14451..14825
                     /locus_tag="KB953_001958"
     CDS             14451..14825
                     /locus_tag="KB953_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBB6866930.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5286 a         2133 c         3045 g         4762 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttaaatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttc aattagtaat tgaagggacg cctaaaagtg aaaaaaaaaa
     7861 tgaaagatga agtattagag tatatcaaaa ataatttaaa gtattacgat ttttctgctc
     7921 aagatattgc aatgaaattt tgtattaaaa gaaatgtcgc ttcccactat ttgaatcagt
     7981 tattttcaga tggaaaatta ctaaagaatg attctgttag accagtaatg ttcaagtata
     8041 atcaacaaaa gccaaaagac tgcttttcta agtttattgg agcagacata tctttgaaat
     8101 ccactataga taaatgcaaa gctactgtaa tgtatccccc taatggcctc ccattaataa
     8161 taaaaggaaa cagtggcgtt ggaaaaagtt ttttagcaag tttgatctat cagtatgctc
     8221 ttgatagaaa agtaattcat aatgacgcaa aatttgttgt tgtgaattgt gcagattatg
     8281 caaataatcc agagctattg tcagcagttt tatttggata taaaaaagga gcctttactg
     8341 gggctgaaaa agatactgca gggatgctat cttctgctaa tggaggatac ctttttttag
     8401 atgaggttca caatctaagt gcagaaaacc aagagaaatt gtttcttctt atggattctc
     8461 aaaagtatcg taaacttggt gaaagtgtgc aatgggagta tgcaaatgtt cgccttatct
     8521 tagccacaac agaagataaa aatacctctt tgttagcaac ttttagaaga cgcatcccag
     8581 ctgaaatcac tctgccagat tacaataata gaagtacatc tgaaaaaatt caattattat
     8641 ttgaatttct taaagatgaa gcaattacga ttgattcaaa aatattttgt tcgattgaac
     8701 tttttactga tcttttatcg aaaacatttg aaggaaatgt tggagaattg aggaatgaaa
     8761 tcaagcttct ttgtgcagaa ggttatttag gaaataagag aaagtcttca atttatttag
     8821 gggacaaact agacagcggt ttttggatag atccagaaat tagtatagat ttaaaaaaaa
     8881 tgtttcttaa atcactttct gaaattgatt tgattgaact ttttcaaaat cacatttcaa
     8941 ttgaagaaag catgagtcaa ttaagagata gaatacttaa agagtgtgca ggttcttctg
     9001 agtataatta tttagaaagt gacgaattta tagcattaag aaactatgtt gttaataaaa
     9061 tatcaccaat tattgattcg acaggtctat gtttattaga tgaattcttg gcggatatca
     9121 gtctatttat aatgtttatt gatgtaattg aagaaacatc aagaaatttt ttgctgtcta
     9181 agttcagaaa atttcgtatg aaaaatgata aacaaaaatt attggcaaat gagatttgga
     9241 gtagtttaga agtcgaagaa agtaaacaag aacttttgtt gaaatggata ctttttcttg
     9301 taaagaagtt ctatgtgaaa ataccagaaa cccattgtct gatagttatg cacggtaaaa
     9361 taacagcttc tgcgatcgca agggaaacaa ataagttgct gaatacatat gtttatgagg
     9421 ctttcgatat gccaattgaa ggtgagacta gtgacttaat taacctaatc aacaattttt
     9481 gtaaaagtat agatacgacg aatggactca ttctgctagt tgatatggga tcattggaac
     9541 aaatgtatga aaaaattgaa tccaatgtaa taggtgattt ggtaatttta aacaatgttt
     9601 ctacagcttt agcgattgaa tgtgggattc aagtttgcca aaaaaagcca attagtcatt
     9661 tttaccagat ggattttgat tcgtttcaag ttaaagttca gtattataaa ggattatcac
     9721 aaaagaaaaa tgtaatagta tcttgtctct ctggtgaggg gatttctgaa aaagttaagg
     9781 atattttaaa acggtatctc gataatcaag tggagatttt gatttttgat tttaacgcat
     9841 taaagaaaat agcaaaggaa aaagatacta tgatttttaa gaatacaatt tgtgttcttt
     9901 caaccactga attttacatt caaggaatcg attgtttaaa tttagaaaat ctaattaatg
     9961 ggaatcaaac cttagaaaaa ttaaataagt atatggactc ggatagctgt gagttctgct
    10021 taaatgaatt agttaaactc tttactattg aaggcgctag tactaagctt cgctttttag
    10081 attccaaaaa agtattcaat gagatagaaa aagtactata tttgtatgaa aaatattacg
    10141 atgtcaaaat cccaagtttt ctgagaatta atctatttct acatctttct ggaatgattg
    10201 aacgaattat gattggtgat ggaataacaa atgaacattt gaaagaggga gcgcaactat
    10261 ttgatacttt tttaaatgta tcaaaagatt ttttttcaga aatcaaagat atttactgta
    10321 ttgaaatacc caaaggagag tatgagttaa tctatacaat ttttgagcag actattttct
    10381 aaatagcata ctagcgtcta aaaaaatgaa agggtgtcca aagtatattt agacgctcat
    10441 ttaattaata tttactgtat tttcccgctt tattggataa tgttagttgg cacagtaatt
    10501 gcttatataa aagtataaga agaaaggaga gaacaaaatg gcgttttggc aaacattttt
    10561 agttgtgttg tatggatttt tcattaactg ggataaggaa gggcccaaaa tcggaacctc
    10621 ccaacctgtc gttgcaggtt tcttgaccgg acttattatg ggagatattc aaactggact
    10681 ttatatcggt gggactttgc aattaatgac actaggtatt actagttttg gtggtgcatc
    10741 agtaccggat tatcaaacag cagcattgat cggtacttat ttagcaatct caactaacca
    10801 ggatgcttct ataggaataa ctttagcaat accggtagca atgttaattg tacaactaga
    10861 tgttctaaag tggtcaacaa acattttttt tcaacaaaag gcagaaaaat tcgcacaaaa
    10921 agaagattat caaaaattag agtggatgca atatttggca tcaattaata caagctttgt
    10981 atctggaatt cctgttttgt taactgtgat attaggccca acgttagtgg gaagtgcaat
    11041 caattatatt cctgaatggg ttaccggtgg tttaaaggta gcagggggac tattacctgc
    11101 tgttgggata ggaatgctac ttaaatattt acctacaaaa gattactttg cctatttgtt
    11161 agttggtttc gttttggcag tttatttcca aataccaatt ttagggattt cgttacttgg
    11221 cgctgcaatc gcactaatcg tatataaaaa tagatcaaat aatactcaag tagcaccagc
    11281 tgcaacagga ggttttgacg aagatgaata agaaactgac aaaaatcgac aaacaagtaa
    11341 ttttccggtg gattctcaat ggtgcaactt cattaaatta tgaaaaaatg gaagggttgg
    11401 gttatgcgta ttctatgctt ccttttatca aggacacata taaagatgat cctgaaggaa
    11461 tgaaacaatc cgttttagct cacttacaat tttttaacac tacgccgtat acagcgcctt
    11521 acattattgg tatgaatgtt ggaattgaaa aagaagaaaa aaataagtct ttggaagcag
    11581 taagtgctct gaaaacaggt ctaatgggtc ctttagcggg aattggagat agtgtatttg
    11641 tagttattcc atggactatt tttggagcaa ttgcagctaa tatggcattg gaaggtagtt
    11701 ttgttggaat tattttgtgg atactggcat caattttatt aaggattact gcttatcctt
    11761 tataccttgc tgggaaagaa tcgggtgcga gcctactaac tcaaattgaa acaaaattaa
    11821 agtctattac tgatgcagta tcaattttag ggttaatggt ggtcggtgca ctcattgcca
    11881 cagttgtaaa agtgaatgtt ggctttgaat ttaaacaagg cgacttaaca atgaccggaa
    11941 atgaaatctt tgatcaagtt atgcctggtt tattacctgc atgtgtagta gcaattgtgt
    12001 attatttgtt aggacgaaag gttaaaccag tttatgtaat tctattaatc ttagtagttt
    12061 ctattgcgtt aagagcgtta aatattttga agtaggtgtt gaatatggat gaaaaaatga
    12121 aaggtattat acatgttcgt attgatgatc gaatgattca tggacaagta gcaacacaat
    12181 ggacaaatca attgagtgct aacagaatta tggttattaa taatgaagta gccaatgatg
    12241 acgtaaaaaa agctgtagtt cgcctcgctg caccacctaa tgttagaact tcaataatta
    12301 caagggaagt agcagttaaa aatattcttt ctggtaaata tgaaggacaa aaagttctta
    12361 ttgttgcagt ttctccaatc gatgttaaat ttttgattga taacggttta ccaataacaa
    12421 gtgtaaacgt aggaaattta tcaagaagga atggaacaga acgcatccgt ccgacgataa
    12481 atattactga agaagagaga gaggctttta aagaactaat tagtgatgga gttgaagtga
    12541 cagtgataca aactcctaga gattcaaaag aatttttaaa ggattttatt tattaagaac
    12601 agaggatgag tggatggagt ttataattac atctcatgga aatattgcca ttggatttgt
    12661 agatacatta aaaatgtttt ttggcgataa tttaaattgt catgtggtaa cacttggaga
    12721 tgcaggcatt gaagaattcc gtgaaaacat tgaaaaaata gtttctcaag taaaaaatca
    12781 agaagttatg gtatttgctg acttgattgg tggaacacct ttcaacgaat ttgctaaaag
    12841 aagtgaagta tttcaaaatg ggtttcaact tcttggtggc atgaatttag gaattttgat
    12901 tgaggctgtc aatttgagat tacaaggaaa aaaaagcgat gatatcatcg aaaatctaag
    12961 aaatatgaat acaattgcgt gtcttgcaga tatgcaaaga catactaacg aagaggatga
    13021 gtgataaatg caaaaaattt tttacaagcc taaagatttt tggtttgggg attgcatgcc
    13081 tttttataaa gatggaactt tttatctatt tcatcaacga gatacgagaa atcctaaacc
    13141 tttaactgat ccttttgggt ggtcactagt tacaaccact gattttgtga aatttgaaga
    13201 atacggggaa gtaattcatc gtggtggcaa cgatgacatt gatcaataca tttacgctgg
    13261 aagtattgtc caaggaatca atgagtattt agctttgtat actggacata atcgcaaagc
    13321 taagttggat ggaaaaactt cagaagtgtt aatgattgca agttctaaag atttaattca
    13381 ttggaaaaag gatggcgaag cagcaaaact tgttcctcaa gaaggatatg ataagaatga
    13441 ttggcgcgat ccttttgcaa tttttgatca agatcatgga aaatacgttt tgatactagg
    13501 agctagaaaa ccagcaagta agaaaaagcc gacggggaga ttggtctatt ttgaatccga
    13561 agatttaaaa gattggaatt tcaaaggaga ctactgggct aataatgaat ttaacatgat
    13621 tgaaatgcct gatatatttg aaattgatgg aacgtggtat ttactttttt cagagtattg
    13681 tgaagataaa aagactaaat atcgagtcgg agcaggatta ttttcagatt ggaaatcacc
    13741 agtagacgaa gctttcgacg gaaaagccta ttatgcagca agaacagtcg gaactaatga
    13801 aactggccgt tttctaactg gctgggtggc aacaaaagaa ggggagaacg atcaaaataa
    13861 ctgggattgg ggcggtgcac ttgttccaca tcaaattatt caaaagacag acaaaactct
    13921 tggtgttaaa ctgcctgaaa aaataattga gaattttaaa gaaatcactc cattttcaag
    13981 ttttaagtta gagaaagaat ttggaaaaaa agaaagagta attgttccta aaatcgaaga
    14041 ttattttcta tttgaaacaa ctttgactat tacatcgtta acaaaggagt ttgctttaaa
    14101 attatttgta gatagtgata ctcaagaagg atacgaattt cgtatttcat tgattaataa
    14161 tcagcttact ttcggaaaaa ctccgaacta tccttggcct caaatgtttg ataaggggct
    14221 gcaacgacca ctatttttgg aaataggcaa agaatatgaa ctaaagttaa ttgttgatga
    14281 tacgatagct gttttatata ttgataatgt tgctttaagt actagaatgt atgataaagt
    14341 cggaacagca ttaagtatga ctgttacgga gggagcaatc aaggtagata aactagacta
    14401 ttcaataaat tatgaatgtg gattttaaaa caattgaagg gatgatgtaa gtgaaattaa
    14461 atggtttaca acatattgga attccaacaa aagattttgg agcttcaaaa atcttttatg
    14521 aatccttagg gtttagtctg attaatgaag aaatgaatgt gggaagtaaa gtcggctttt
    14581 tcgagttgaa tggaacgata atagaaatat gggaagataa aactactgag tgtagaggag
    14641 ccattgatca tctagcatta gatacggatg acatagatga ggtattcaat tcaattcaag
    14701 atttaggata tgaattatta gataaaaata ttatggaatt accgttctgg gaaaatggaa
    14761 tccgttactt taatttctac ggaccaaatc atgagattat agagataagc caaatgaaga
    14821 aataaataga taacgaagtg ctctaagttg gtatgaacga aaagtacttc attttttggt
    14881 atcagcattt caatagttta atgagaattt actatttcaa attaattcaa agtgacagta
    14941 ttttttactt atttggtttc aaattttgta atccttccta tttattggaa tacactccac
    15001 attttgtata caagtcatat catgttttag caaagctctt accaagcaaa gttataatga
    15061 tgttttccaa agtttgatct ttcgatggag gctaacacat attttttgca ggacagccaa
    15121 aattagatgt atagaataga gtctgtgagg cgtatagaca gtttattgtc tgtatgcctt
    15181 tttttatttt gaaaaatttt tagtattttt cgaaaaatgc tcactc
//
LOCUS       DADVXU010000068        15055 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.071, whole genome shotgun sequence.
ACCESSION   DADVXU010000068 DADVXU010000000
VERSION     DADVXU010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.071"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(534..1100)
                     /locus_tag="KB953_001959"
     CDS             complement(534..1100)
                     /locus_tag="KB953_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBB6866931.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1100..2056)
                     /locus_tag="KB953_001960"
     CDS             complement(1100..2056)
                     /locus_tag="KB953_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBB6866932.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2056..2712)
                     /locus_tag="KB953_001961"
     CDS             complement(2056..2712)
                     /locus_tag="KB953_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBB6866933.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3082..5100
                     /locus_tag="KB953_001962"
     CDS             3082..5100
                     /locus_tag="KB953_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBB6866934.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5170..5376
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5527..7941
                     /locus_tag="KB953_001963"
     CDS             5527..7941
                     /locus_tag="KB953_001963"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBB6866935.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8289..9842
                     /locus_tag="KB953_001964"
     CDS             8289..9842
                     /locus_tag="KB953_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBB6866936.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10223..12007)
                     /locus_tag="KB953_001965"
     CDS             complement(10223..12007)
                     /locus_tag="KB953_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6866937.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12022..13743)
                     /locus_tag="KB953_001966"
     CDS             complement(12022..13743)
                     /locus_tag="KB953_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6866938.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13843..14604
                     /locus_tag="KB953_001967"
     CDS             13843..14604
                     /locus_tag="KB953_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBB6866939.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4905 a         2760 c         2969 g         4421 t
ORIGIN      
        1 attgaaacat ccatctggaa tttatctttt ttactgcttt gatcataaaa gtctgcatat
       61 tcatcactga tattttttct aataaattct tccatcataa aaatcacctc aaaacgatta
      121 tacgaactta cgttcttgtt gtaaagcgat atttgagggg caaaaaaggg gcataatttg
      181 actgatatta agttagctat tgatatatga atgacataca aaaatataaa atcttttaaa
      241 aatgcttata tatcaatgtt tttgccattc ttttatatat ctgttatatg tcaaaaaaat
      301 gtgttcaatt tcaatttgta tttgaataaa gaggttgtga aaaagctgtc ctgcatcaag
      361 taataagaac agccaatcaa aaacagcttc tcatgttttt gatttggttg gacttattac
      421 cgcagatata agcttttgag caccgtgaat aaagaggtta tgaaatcaac gctctaacct
      481 gagtattaga gaaaaaaacg caaaacatat catttatgtt ttgcgttttt tatttatctt
      541 tttttctcaa tacaatataa aataggcggc tggttctttt ggttgataaa ccgataattc
      601 agcacattat atgtttcctg tggcagtttg gaacaaaaag aatcaaccat atccagttct
      661 ttttctccac cttcatgacc atagtagacg acaaggatca atcgtccgcg aggcacaagt
      721 cgtttcaata tttcttccat agctgtgcgt gtagtctctg gcatagtgat gattgcttta
      781 tcactttttg ggagataccc tagattgaaa atccctgctt tgagattttg ttgttcagct
      841 atcacttctc ctaacgtttc gtggccttgt agaaacaatg agacacgttc ttctaactga
      901 cgctgttcta gcctttcacg agtagcatcg attgcttgtt gctggatatc aaaactatag
      961 acatgaccag tttttccgat gtgttctgct agaaaaaccg tatcgtggcc attgcccatc
     1021 gttgcatcta cgacgtgatc ccctggttgc aagatttctt gcagtaatgt atggctgaaa
     1081 tgcaaagcag tttgcaacat cagacgctca cctttttccg tacatcatat tttccttgga
     1141 agctatctct gcgaagaagt tcttcatcaa ttgcattcaa gacttcccat tttttcaagc
     1201 tccacattgg tccaatcaag gaatcccacg gcgcatcccc tgtcaaacga tggatgatga
     1261 tctcttttgg tatcatttcc agctggtcgc aaatgacatt gacatagtca ggacggctca
     1321 ttaattgaag gcgaccttca tgatagtccc gcaacattct cgttttgcgc atcagatgca
     1381 tcaaatggag cttgattcct tggatatctg aatctaaaat ggtgcgtctg acattttctt
     1441 tcatcatttc taaactttcg cctggtaatc cgttgatcaa atgcgtacaa acacggatat
     1501 tatgtttgcg taatttagcc acgccatcta aataagtttg ataatcatgt gctcggttga
     1561 tcagttttga agtttcttca aaagttgttt gcaatccaag ttctacccat aaatacatcc
     1621 gctcgttcag ttcagcaagg taatcaacta cttcatctgg cagacaatcc ggtcgtgtac
     1681 cgactgataa tcctacgaca cctggtaaat tgactacttg ctcaaatcgt tctttgatta
     1741 tttcaactgg cgcatgggtg tttgtaaaat tttggaaata aacgatgtat tgttggacat
     1801 gaggccattt ttgatgcatc atgtcgattt ctttctggaa ttggattgga agagggtctt
     1861 caggtgcaac gatcatgtct cccgaaccag atacactgca aaaagtacaa ccgccatggg
     1921 caacagtccc atctcgattc ggacagtcaa atcctccatc gatcggcacc ttgaaaattt
     1981 tcccgccaaa ctcatttcgt aaagcgtaat tccaagaatg atatctttta tttccttctg
     2041 aataaggaaa agtcattaaa agtccttctt tctgtaagag ttatctattt gttttctttt
     2101 ttctctatac actggataag tcaggcacag ccaagaaaga ctgagtaagt aaccgccgat
     2161 gatatcagta ggaaaatgaa cgcccagata aatgcggctt atcccgacac ttactataat
     2221 aaacatcatc agcaattgaa gcaatgtgcg taaggttctt ttttgaacaa aagctggaag
     2281 cagaaatagt aaagaaccgt aaagaatcat actgcctgct gcatgtccac tagggaaact
     2341 gtaacttgtt tcaactacta agtgcgccaa tgaaggacgc tctcgcatga aaaatagttt
     2401 gatcaccggg ttgatgattc cagcaattac aataaatcca gaaacgaacc aaaatgtttc
     2461 tgcccttttt ttcttatacc acatccataa agccgcacaa gcaaataaaa tgatgacggt
     2521 aagaggatta ccgaatttag tcataaagag ttggtagctg ttcagatcag gataagaagc
     2581 tctgacaatg cctgtaaacc actgatcgaa accagaaagc cacgacagat ggaactttac
     2641 gatccagccc aaaatgaaaa acaggacaag aaagaagcta ccagccacct gaaatgatgt
     2701 tttatatttc atacaattcc tcctttgaag caatgactta ttatagcatt tttggtccag
     2761 cgaaaaaaac ggtaaagaga taaaaccaat gtatgattcc tctttgaata accaatattc
     2821 ttataaaaaa aataactgta atcggaaaga cattttttgg taatgcaaat gaaatgttag
     2881 ggaaataaaa tcagtgtttt tcgaaatcct tttaaacaag aacgtagacg taggaagaac
     2941 ttgaaaatga acagaagttg ttcattacgg taaattacaa tatgattaca aaaaacaaca
     3001 aagctcttaa ataactttta ataaatatta agagaagtta taattattaa taatagataa
     3061 attgaagttg gaggatagaa gatggaaaac attgcaagaa aagaacggag acgtttagaa
     3121 cagaccaaac gttttcgaaa agcaaaacga ggggcagcaa tcgtgggaac tgcgatggta
     3181 ggctgttctg tcgctgcccc attagtacag ccggttcctg ttcaagcgga tgaaacacct
     3241 gttcagttcg gcgcaagaat caatacagcg gcatttatca cagaaattgc tacttatgct
     3301 caaccaatcg ctcaagccaa cgatctatat gcatcagtga tgatcgcaca agcagttgta
     3361 gaaagcggtt ggggcagtag tgcgttatct caagcacctt attataatct ttttgggata
     3421 aaaggcagct atcaaggaca aaccgtctac atggatacgc ttgaattttt aaataatcaa
     3481 tgggtaaata aaaaggagcc tttccgacaa tatccttctt ttgctgaatc tttcaatgac
     3541 aatgcctatg tacttagaaa tacttcattt ggcaacggtt attactatgc cggtacttgg
     3601 aagagcaaca cgaaatccta tacagatgcg acagcttgtt tgacagggcg ttatgcgact
     3661 gatcctggtt acgcaggcaa attaaataat atcatcgcaa cttacggatt gacccaatat
     3721 gatacaccag ctagcggaaa cgcaggcggt ggagcgacta caggtggcaa taatactgga
     3781 agtgcaggta gtactggcgg ttcaagcaat tctggtaata caaactcagg ttcagcaact
     3841 tcgaccactt atactgtcca atctggtgat tctgtttggg gaatctcaaa taaattcggt
     3901 atcacaatgg atcaattgat ccaatggaac aacatccaaa acaatttcat ttatccagga
     3961 caaaaactga caatcaaagg tagccaggaa aatggctctt cgacgaataa ttccggtaac
     4021 aataccaatt cttcaggaaa tgctggtaca tcaaacggag gacaaacaac tggtgcgaaa
     4081 tacactgttc aatctggtga ttctgtttgg aagatctcaa atgatcacgg aattacgatg
     4141 gctcaattga ttgaatggaa caatatcaag aacaatttcg tttatccagg acaacagcta
     4201 atcgtatcaa atggcggttc ttctaatgct ggagcaaata ctggctcaaa tactgggaat
     4261 acaagtggct ctagttcgtc cagctcaagt tcttctaata cagcttcagg aacgaaatat
     4321 acagttaaag ccggagactc tgtttggagc gtctcaaata aatacggaat tacgatggct
     4381 cagctaatcc aatggaacaa tatccaaaat aactttatct acccaggaca acaactgact
     4441 gtttcaaaca gtggatcaac aaacagcacg tcaggtaata caggttcgtc aaccaataca
     4501 aataacagca gttcagttaa tactggcagc agttcgtctc aaggcactta taccgtgaaa
     4561 gcaggtgaat ctgtttggag cgtggcaaat aaaaacggca tcacgatgga tcagttaatc
     4621 gaatggaata atatcaaaaa caacttcatt tatccaggac agcaattgat cgttaaaggt
     4681 ggaacttcgg caaatacaaa tacaggttca actacttctg cagcgaaacc aaatacaccg
     4741 aatacgtctg ctacgacaag tacatcatcc actggtaata cgatgtatac cgtgaaagcg
     4801 ggcgagtcag tttggagcgt agcgaacaaa catcatatca cgatggatca gttaatcgaa
     4861 tggaataata tcaaaaataa tttcatctat ccaggacagc aagtgattgt gaagaaaggt
     4921 tcatctcaat caacaaacca acaatcagtt actggttcga aaacctatac tgtgaaatct
     4981 ggagaatctg tttggagtgt tgctgacagt catggaatta caatggctca actaatcgaa
     5041 tggaacaata ttaagaacaa ctttatttat ccaggtcaaa cgttgattgt agctaaataa
     5101 aatagctgtt cgaagaaata acgattgaat tccacaaaaa tatttgttac tataaggcta
     5161 cttaaatatg acaacacttg aaaaggaagt agtaaaaaga ctgaagtttc agagagcgca
     5221 tggctggtga gaatgtgcac ggatgctttt gaactcgcct ttgagttgcc cttgctgaat
     5281 acccgagtaa gcaagcggcg gtgacggccg ttacaacgtt cgaggccttt tcactaaggt
     5341 aaaattaggt ggtaccgcgt agattacgtc ctataaaaag agcaatcttt ttataggatt
     5401 tttttatttc ttttgtgaag tttgagaaaa caaagcaaaa ggaaataaaa ataaacagat
     5461 tctgccggtt ttttcgttat atttataata gaaacaagtg ttttcaatag aacaggagga
     5521 cagcaaatga gttacaatca taaagaaatt gagaaaaaat ggcaaaaata ctgggcaaaa
     5581 acgaatactt tcaacaccca tgatgatcct gaaaagccaa aattttacgc actggatatg
     5641 tttccctatc catccggtca agggctacat gtagggcatc ctgaaggtta tacggcaacg
     5701 gatatccttt cacgtgtgaa aagaagtcaa ggatacaacg tgctgcatcc aatggggtgg
     5761 gatgcattcg gtctgcctgc tgaacaatat gcgctagata caggaaacga tccagctgag
     5821 tttacccaaa aaaatatcga aacattccga cgccaaatca attctttagg atttagttat
     5881 gactggaatc gcgaagtgaa tacaactgat ccggagtatt ataaatggac acaatggatc
     5941 tttacgaagc tttatgaaaa aggtctagct tatgaagcag aagtagcggt aaactgggta
     6001 cctgaattag gaacagttat ttcaaatgaa gaagtgatcg atgggaaaag tgaacgtggt
     6061 ggctatgatg tagtcagaaa accaatgcgc caatggatgc tgaaaatcac tgcttatgct
     6121 gatcgcttat tggatgatct agaattagtt gattggccag aaagcattaa agaaatgcaa
     6181 agaaattgga tcggtcgttc agtaggagca aatattgaat ttaaagtggc tggtacggat
     6241 aaatcctata ccgtatttac gacacgacca gatactctat tcggtgcaac ttactcggtt
     6301 cttgcacctg aactagatct tgttagagaa attacgaccc ctgaacaaaa agctgctgta
     6361 gaagcttata tcgaagagac agctaaaaaa tcagatctaa agagaacgga tcttgcaaaa
     6421 gaaaaaacag gagtatttac tggtgcttat gcaatcaatc cagttaatgg caaggaaatc
     6481 ccaatctgga tcgccgatta tgtattatct tcatacggca caggagccat catggctgtt
     6541 ccagcacatg atgaacgtga ctttgaattc gctcaaactt tcggactaga aattcttcca
     6601 gttatcgaag gtggagatgt tgaaaaagct gcttacacag aagatggtac gcacatcaac
     6661 tctgaatttc tagacggcat gaacaaacaa gaagcaatcg ataaaatgaa tgaatggcta
     6721 gaagaaaatg gcgtagggaa aaaagaagtc agctatcgct tacgtgactg gctattctcc
     6781 cgtcaacgtt actggggtga accaatccca atcatccatt gggaggacgg aacagtcaca
     6841 gctgtcccag aagaagagct accattacgg ttgcctaaga caaaaaacat caagccaagc
     6901 ggtactggag aatctccatt agcaaatatc gaggagtggg tcaatgtcgt tgatcctgta
     6961 acaggtaaaa aaggccgtcg cgaaacgaat acaatgccac aatgggcagg aagctcatgg
     7021 tattacctgc gttatatcga ccctcataac aaaaaagaac tagcagatta tgaaaagttg
     7081 aagcgttggc taccagttga catctacatt ggtggagcag aacatgcggt acttcattta
     7141 ctatatgcac gattctggca taaattctta tacgatatcg gtgttgttcc tacaaaagaa
     7201 ccattccaaa aactatacaa tcaaggaatg atcttaggcg aaaacaacga aaaaatgtca
     7261 aaatctcgtg gtaatgtagt caatcctgat gatgtggtcg aaacttacgg tgcagataca
     7321 ctgcgcatgt acgaaatgtt catggggcca ttagatgctt cgattgcttg gagcgaaaat
     7381 ggcttagaag gaagccgtaa attcttggat cgtgtatggc gtttgatcgt tgatgagaac
     7441 aataaaatgc gtgaccgtat cactacattg aatgatggca agcttgataa agtgtatcat
     7501 caaacagtga aaaaagtgac agaagactat gaaaatcttc atttcaatac agcaatctcc
     7561 caattgatgg tctttatcaa tgaagcctat aaagtggacg ctctgcctta tgaatatatc
     7621 gaaggatttg ttcaactact cgcaccgatt gctcctcaca tgggtgaaga actatgggca
     7681 atccttggaa atgatggtgg tatttcttac gcaccatggc caacatacga tgaagcagca
     7741 cttgttgaag acgaagtaga agttgttttc caagtaaacg gtaaagttcg tgcgaaaagc
     7801 aatgttcccc gagacttagg aaaagatgag ctagaaaaag cagcattagc caatgaaata
     7861 gtgcaagaat atattgaagg caaaacagtc agaaaagtta ttgcggtacc aaacaagtta
     7921 gtcaatatcg ttgctaatta attggctctt tgtcaataag gactgatgag ttgtgcaaaa
     7981 tcaaatctgg gccagaatga acttcattct gtctcagatt ttttgttatt tcaaattgat
     8041 taattgattg atcaaaaagg ttttgatttt catgttctca aacacgttca atgtcttttt
     8101 cttctgaatg gtcttgtaag agatctctgt aatcatcaaa tttttgtcta ttaatcttag
     8161 tattttttga tgtataaact acctgaaatt gagagtacat accaaattca taaatttttt
     8221 attagtggga ctagtgattg tatggatttt gacgcataat gaatgaagta tgagaaaagg
     8281 agagacaaat ggatcaagaa ctatttatta tcgaagtatt atcagctaaa caaaaaatta
     8341 tcgagaaagc gaaaaaaaga gcgaaaagaa taatgttcaa gcttttaagc ttagtatttt
     8401 tctctggttt gctttttttc ggagctgtat cgttcctata tcgccatatg agtgcagtag
     8461 tccgttcagc tgaagggatg caaaaattaa ataatttgta tcacttttcg tggggagttg
     8521 gcggaataac aattgttgtg cttattgttt ttcttctact tatttggctc cgttaccatc
     8581 aattgtcgcg tcatttcgat caatggctta gtcaaagagc acaacagttt ttacataata
     8641 aaccaacttc tcaagatgat gactatcttt attttgagaa gacttcgacg agtcaagctt
     8701 ttcggataca aaaaagtgat tgccagttat tgctgagatc acttgatgat ttacctatct
     8761 acttaggcga aagcaaatca aaattcggaa tgaacacatt ccatattttt ttactagaaa
     8821 acgaacccag atgggaaaaa agccagtctt cgcctggtgg atacctgaca actaagatga
     8881 gtacattagt cactggtttt ttcctcattc ttttagctgg aacaatctat tatgggagat
     8941 acactcagcc aaaaatgtct tctcatataa atgctgcttc tcacatgaca atgaactcaa
     9001 ctaacgatac gcatatacaa aagacaaatg aagggttgtt agctgatcct aagcaaaagg
     9061 taaatttgac aacacaaaag ataaatgatc tcaaacttga tacagagacg cactctctct
     9121 atatgacaac agatagtggt gctaattggg tatttgtgcc gattgattcg caatggttgc
     9181 gttttggcga atacaccctg acaacggggc tgattcctga aggttattgg atggacaaca
     9241 catatgatat ttccgccgat ttctcatggt ttatctattc ttctggggat gatgtacgtc
     9301 tgctgacttc aagagataat ggtaaaacat ggcaaacgtc tattatcacc caatcagttc
     9361 agccgatacg ttatcgaaaa gcggtatttt atggcaacga tggtctattt atctgtacat
     9421 cagccagtgg gatgtctgca gaaactttgt acatttacac tactatggat ggcggaatca
     9481 cttggataaa aagtggaagt acaatgatca atcagccagt acaaaatgtc agcttcgtta
     9541 caacttcttt aggttttgtt tcgacacgtg aaagtttgtt ttatacaaac aacggaggac
     9601 gctcattcaa agaatcagtt ctgtcgattc cggaaaacta tcagacaggg gggatagatc
     9661 tttttcaatc accaaatgaa gttactcaaa taagcgcgaa tacgttagag acaaaatttt
     9721 atttgttgaa aaaaggacca atcgatgcag ggaaaatgtt tgcctgcctc ttccgttcaa
     9781 ctgataacgg ggaaacatgg caattcgttc agcaactatc gcaagtagaa caaggagatt
     9841 aaagaagaga gattgtgagc ctgacgttca gcaccgagtc acaagaaaca aattcaaaaa
     9901 acagcttctc atatttgttg aatagtgtaa ctcgtgattc ggagttgaca agagcaacaa
     9961 gaagcgattt tgaagtatag cttctcgtat tttacaagat cgtgtcttat tgccgatagg
    10021 ttgtctcttg aacaccgttt attcgtaaaa agggattgtg acaagtcttt tgtcactact
    10081 taccgaacga aactgaagga gctatccaaa aagtatcatt agtattattc tttgtgtttc
    10141 tagtggcacc actagtacat ttaaaaaagc tatcgatcat ggactaatca tacatgatcg
    10201 atagcttttt agataatgtt tgttacgcta tctgaaattg actattataa agactggcat
    10261 aaagcgttgg tgattggagt agttcagaat gtgttccttt ttcgacgata tcgccattct
    10321 tcattacaag gatcatgtct gcattttcaa tcgttgacaa acggtgtgca atcacaaaac
    10381 ttgtcctatc ttttgtcaca gcttccattg cttgctgaat caatgcttct gttcgtgtat
    10441 ctacgctaga agttgcttca tctaaaataa cgacaggagg attcgctaga atgactcgag
    10501 caattgtcaa taattgttgc tgtccttgag ataacgtgcc gttttcgcta gaaatgatcg
    10561 tatcatatcc gtctggcagt gttcgaataa agtgatcaca ttgtgcgatt tcagccgctt
    10621 cgataatttg ttctcttgtg gcatccattt tcccgtaggc gatattatca gctaccgttc
    10681 cttcaaacag ccaggtattt tgtaaaacca tcccaaaaga acgacgtagt tctccacgtg
    10741 aaagcttggt aatatcaaga ccgtcgaatt cgatctttcc tttattcagt tcataaaaac
    10801 gcatcagcag attgactaaa gtcgtttttc ctgcaccagt tggaccaacg atcgctaccg
    10861 tttgttttgg ctttactgaa aaatcgactt gattcatcaa tattttatct gatgtgtaac
    10921 caaactgtac atttttgaaa gctatttttc ctttcggttg ttctaaaagt cttgcatctg
    10981 gaaggtctgg aatttcatct gtttcatcta atatctcaaa tatcctatcg attgctgcca
    11041 ttgcggattg gatcgagttg atcacataag aagcggtaga aattggttca gaaatctgat
    11101 tgatgtactg aagataagct tgtaagaagc cgatcgtcac accacctgat agaacaagca
    11161 tagcaccgaa taccgcactt atgatgaagg cgagttgatt aatgaaacga atggccggat
    11221 agatagcaca gttcataaat tgagcacgtt taaaagcgtt gtattgcttt ttatttactt
    11281 cgtgtatagc agcttctgca tgagcttgct gattgaatgt tttgaccact agattaccag
    11341 ctaagatttc ttccattcga ttatttaaaa cacccagttc atactgattc tgttcagaaa
    11401 tctgtttgtt tttgttcgca atcttagttg taacaattga actgccaacg atcagcaata
    11461 agacgagcaa agtaagcttg ctttcgatat aaaacagcat cagaccagca gcagctattg
    11521 ttacgacaga agtaaaaaat tgattgatac ctgtcaataa cacttgagaa agttgattga
    11581 gaccggtagt tgtccgacta atgatgtcac ctacttgatg attgtcaaaa aacgacatcg
    11641 gtaatgtttt gaattttttt gtgatttctt ttcgcaccct caatgtgatc ttttcgctta
    11701 acgctgccat tgtccgttcc tgaataaatg aagtgatgct gcttaagact gaacagccga
    11761 tcagcaataa gacagggaac aacaatgctt gtttgatatc actgaaagaa agctgtccaa
    11821 tacctgtttt tcttatttct gataagagat catcgatccc gattcccatg atcaagggca
    11881 tggctacgac taataggttc ccgaataaac tgcatagaaa caatccgtaa aaatatttat
    11941 gttcaggttt gatcattttc caaaaacgta tcaaagcacg cgtgttcaac gtttttttct
    12001 tttccatcgt atcccttctt tctatattcc ttgtgatttt gcaaactcct gataagtttg
    12061 gcaattagac agtagttctt catgagtacc ttgtccaacg atttttcctt catctaaaac
    12121 aatgatgtta tctgcttgtt ggattgtact gaggcgttga gcaacaatca cgagagtttt
    12181 ctccgacatg tgtttatgaa gggctgctct taatgctgca tctgttttat agtctaaagc
    12241 ggagaaacta tcatcgaata catagatctc tgctggtttg atcaacgctc gagcaataca
    12301 tatccgctgt ttctggcccc ctgaataatt agaaccgcct tgagcaacaa agctgtccaa
    12361 tccgtcagga agggtgtcca ctagttcttc taattgagcg atcttgatcg ctttagacaa
    12421 ctcttctgtt gaggcatatt catttcccat ctgcaagttg cttaaaattg tcccgctgaa
    12481 taagaaagcc ttctgaggga cataactgat gtgggagcgg atctgttctt gagtcatttg
    12541 agtaagagga ataccaccaa gggttacttt tcctatcgta gggtcactaa gtctcaagag
    12601 aagtttcgcc aaactacttt ttccagatcc ggtcccgcca acgatcgctg ttgtttttcc
    12661 tttaggtatt gtgaaatcaa tatcttctag aactggatca gagatttcag tatatttaaa
    12721 tgtgacatgt tcaaaagcag ctgcatttgt ctgatcaaag gttggttcag ctactgtttt
    12781 gatcgtctct tcctcaattt cgatctctgc ttgcagtatt tcttctatcc gtcgtaatga
    12841 ggcaaacgat ttagggataa tgaccaatac catagctgcg atcatcaagt aagcaagtgt
    12901 cagcatagag tattcgacta cagcagtcac gacaccgatt tgtaatgctc cttctgctac
    12961 aagatttccg ccaaaccaca aaatagaagc ataaacgatt cccatgagca aaaaagcaat
    13021 tggagtgacc caagcaaata ttcggttcac tttgatcatt tgattagcat agtcagcaaa
    13081 gctcttattc gttcgctttt cttcatatga ttgattatca aaggcacgga tcatattaat
    13141 cccagtaaag aattctctca atgtgatggt gatccgatcc atttttggct ggatactcaa
    13201 agataaaggt gcccctcgtt tcatcagtcg ataaacaatc catacataaa aaacaacaga
    13261 gatcaatgta acccatgtta gtaatagaga ataacgaaac atcatgagaa tggtgaaaac
    13321 gcaaataacc ggtgctggta agatcatttg gcaaaataaa acaatcattt gctggacatt
    13381 atcgacatca ttcgtcattc ttgtcaaaag agatccagaa ctaaaacgac tgatatcctt
    13441 cattgacagc tgttggaatt tacggaaata tttctcccgc gtttggaatc caaacctagc
    13501 ggaagccaat gctgaaaagt aactagaggc aactgcagcg attgttccta gaaacgctac
    13561 aagcagcatt tcaactccaa ttttttttat aactgaggct tcgccacttg ctatacctgt
    13621 gtcgatcagc tttgctacta attttggtac acctaatgta ccggcgattt ctaaacagat
    13681 catcacaaag gtaaggagag cgagtccttt gtttttctgt aagaatgttt ttaagataga
    13741 catattattc ctcctttttt cctgatactt ttcgattcta taccttcaag taacttgaag
    13801 gtcaatagaa aaaagaataa actgaaaaag aggtgagaaa aaatgaaaaa aatgtatacg
    13861 attggccaag tatcggaact atttgatttg ccaaaatcaa cgatccgcta ttgggatgaa
    13921 caaggattaa tccgttcaag tagacaagaa gagaatgatt atcgattgtt tgatatcgat
    13981 gatattttta tgctttatga tatcgttttt tatagaaaat tagatatacc gatcaaacag
    14041 atgaagaatt tatatggaaa aacattgacg gagttatatg agacacttga tgaaacagaa
    14101 agaagaatcc ataaagaact ggtggtgatg aaacaaaaac aaaaagagat aagggagcaa
    14161 aaaaaacagt tgaaattgat gatcgataca aacgaggaag aatttcctgt agaagagatc
    14221 ccttttgact gtatgatctc aacggagttt gaagatattg tggaaatcaa aaaatttcta
    14281 cctaactatt caagctttgg gatgatgagt atgccagctt ccagttcaca gacgatgtat
    14341 ggatttttta tcgaccctag tgaagtacat ttatttactc aagacgtgat ttgggagaaa
    14401 aaagatacgg ctgtttatcg tcgattctta ttgaaatccg agatgaacca tgcggaacgt
    14461 aataatattt tagaaatcag agaaagaatg tatgaaaaag gatggaaaac aggagaagtg
    14521 atcggccagt atctattgac gaatacggat gaaaacaata tcagaactga atattatcat
    14581 gcatggatag aaatgaaaaa ataggaaata tgtgtttaag ataactcgaa aatgatggtt
    14641 gttttagtta taaagaaaaa ttttctggtt cgattacggg tttaatgtgg gggattatta
    14701 gtcgtggata gatagaattt tagtttttta gaggaaatat gtctttaaaa atggaatggt
    14761 tgcaattctt agcgatatat acgatgtaat tgtcctctta aaaaagtatg gaaaaataat
    14821 tgtattgggt gttatgtata attcatggtg aactgagact aatgagaaga aaaattcata
    14881 ttgttatgag tgagattgta tcagatgaga atgataaatt aaataggtgc ataaaagcat
    14941 tacacttaaa atacattgtt gcttacatta ttacaatatg ttaataaggc aaaaataaat
    15001 agatggattg aataaacata ttttttaatc aaatagtccc tactgtgact ttcct
//
LOCUS       DADVXU010000069        14274 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.072, whole genome shotgun sequence.
ACCESSION   DADVXU010000069 DADVXU010000000
VERSION     DADVXU010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.072"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(41..3715)
                     /gene="rpoC"
                     /locus_tag="KB953_001968"
     CDS             complement(41..3715)
                     /gene="rpoC"
                     /locus_tag="KB953_001968"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBB6866940.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(3773..7390)
                     /gene="rpoB"
                     /locus_tag="KB953_001969"
     CDS             complement(3773..7390)
                     /gene="rpoB"
                     /locus_tag="KB953_001969"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBB6866941.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8118..9086
                     /locus_tag="KB953_001970"
     CDS             8118..9086
                     /locus_tag="KB953_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBB6866942.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9104..10009
                     /locus_tag="KB953_001971"
     CDS             9104..10009
                     /locus_tag="KB953_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6866943.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            10006..10812
                     /locus_tag="KB953_001972"
     CDS             10006..10812
                     /locus_tag="KB953_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBB6866944.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            10948..11937
                     /locus_tag="KB953_001973"
     CDS             10948..11937
                     /locus_tag="KB953_001973"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HBB6866945.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDVK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12254..12778)
                     /locus_tag="KB953_001974"
     CDS             complement(12254..12778)
                     /locus_tag="KB953_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HBB6866946.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13065..>14274
                     /gene="scm"
                     /locus_tag="KB953_001975"
     CDS             13065..>14274
                     /gene="scm"
                     /locus_tag="KB953_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HBB6866947.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTS"
BASE COUNT         4047 a         3087 c         2815 g         4325 t
ORIGIN      
        1 gttgtgacaa taatttgtca caacctcttt tttaaaatat ctattttgtt tgattgttca
       61 ttgcgtcttc tgcagccatt tgcgcttcaa tatctgaaat actgtagaca ttttcgcttg
      121 ctacaccgac ttcttttggt tccatgtcac gataacgttg cataccagta ccagctggga
      181 tgatcttacc tataataaca ttttctttca aaccaagaag aggatctttc ttaccgcgga
      241 tcgcagcatc cgtcaagaca cgagttgttt cttggaatga tgcagcagat aagaaactat
      301 ttgtttctaa tgatgctttt gtgataccta gtaagacagg tcgagaagtt gcaggaacgc
      361 ctcctgagac tagcgtattg tagttttgat ctttaaagtc tgcgatgtct aataatgtac
      421 ctggtaagat atctgtatca cctggatcca ttacacgaac tttacgcagc atttgacgaa
      481 ccattacttc gatatgcttg tcgccgattt ctaccccttg catacggtaa acacgttgta
      541 cttcacgaag caagtagttt tcaacagata atacatcgcg aacttgcaac aattgttttg
      601 gatcgattga tccttctgtc aatggtgcac cacggtggat catatcgcct tctgctactt
      661 tcatacgtgc tgtataagga acagtatacg tacgtgtatc tgtttttcct ttgattgtta
      721 cttctttggt acgtgttgca ggatcttctg agatatcgat gacatcccct gttacttctg
      781 tgatcactgc ttgccctttc ggattacgtg cttcaaagat ttcttggaca cgaggaagac
      841 cttgagtgat atcgtctccg gcaaccccac ctgtatggaa tgtacgcatc gtcaactgag
      901 taccaggttc accgattgat tgagcggcga tcgtaccaac tgcttctccg acttcaacgt
      961 cagagccagt tgcaaggttg cgtccgtaac aatgtttgca gacaccatgt tttgtattac
     1021 atgtgaagac agaacggatt gtcactgttt cgacacctgc atcaacgatt tgttttgcga
     1081 tgtcttcgga aatcaattgg tttcctgcaa tgatcaattc atttgtttca ggatgacgta
     1141 cttctttacg ggtgtaacgt cccactaaac gatcttctaa tgattcgatg atttcatttc
     1201 cctcgcggat ggcttggatt tcaagacctc ggtcagtacc gcaatcttct tcacggatga
     1261 ttacatcttg ggccacgtca accagacgac gagtcaagta acctgaatcg gcagtcttca
     1321 aggctgtatc ggtcatccct ttacgagccc cgtgagttga gataaacatt tccaagacgg
     1381 atagtccttc acggaagttc gaaatgattg gcaattccat gattcgacca ttaggagcgg
     1441 ccatcaaacc acgcattccg gcaagctgtg taaagttgga aatgttacca cgagctccag
     1501 aatcagacat catgaagatt ggatttttcg cttccaaacc ttccatcagt ttttgttgga
     1561 tgctgtcttt tgcagcattc caaacagcaa tgacacgttc ataacgttcg tcatctgtga
     1621 tcagaccacg acggaattgt tttgtaatag tttcaacttg tttatgggca ttttcaatga
     1681 tttcttgttt ttcattcagt acactgatgt cagcgatccc tactgtgata ccggcatgtg
     1741 tcgaatgttt gtaccctaag tctttcatac ggtcaagcat cttagaagtt tctgttactt
     1801 tgaatcgttt gaagacttct gcgatgatgt ttcctaagtt tttcttcttg aacggtaata
     1861 ctaattcttg ctctttgata tgtgcaggaa tatcagtacc agcttcaaca aagtatttgt
     1921 ccggtgtttg gacggtcaag ttgaagtctg ttggttcatt cagataaggg aattcaggag
     1981 gcatgatctc gttgaagatg atcttaccaa ctgttgtgat catcgtacgt tctttttgcc
     2041 attcagtaaa tggtttttca cctaatgagt tagggttcac accgatacgt gaatgtaaat
     2101 gaacatatcc gttgcgccat gcgataacgg cttcgttttg atctctgaag accattcctt
     2161 cgccttcgcg tccttcttct tccattgtca agtagtagtt acctagaacc atatcttggg
     2221 aaggagtaac aactggttta ccatctttag ggttcaagat gttttgagca gctaacatca
     2281 acatacgtgc ttctgcttgt gcttcttcat tcaatggaac gtggacagcc atttggtctc
     2341 catcgaaatc ggcattataa gcttcacata caagtggatg caaacggatt gcgcgtcctt
     2401 gtactaaaac tggttcgaat gcttggatac caagtcggtg aagcgtaggt gcccggttca
     2461 gcaatactgg gtgttccttg attacgtctt ctaaaacatc ccatacttcg tcttcttgac
     2521 gttcgatttt acgtttcgca tttttgatgt ttgaagctag ttcgcgttga acaagctcac
     2581 gcatcacgaa tggtttgaat aattcaatgg ccatttcttt tggaagtcca cattggtaca
     2641 tcttcaagaa aggtccaaca acgataacgg aacgacctga gtagtcgaca cgtttaccta
     2701 gcaagttttg acggaaacga ccttgtttcc ctttcaacat atgagaaaga gatttcaatg
     2761 ggcggttacc tggtccagta actggacgtc cgcgacgacc gttatcgatc aatgcatcta
     2821 ctgcttcttg cagcatacgt ttttcatttt gaacgatgat tccaggtgca ttcaagtcta
     2881 acaaacgttt caaacggtta ttacggttga ttacacgacg gtaaagatca ttcaagtcac
     2941 ttgttgcaaa acgtccacct tctaattgaa ccattgggcg taaatctggt ggaataacag
     3001 ggataacgtc cataaccatc cagcttggtt gatttcctga agcgcggaat gcttctaaaa
     3061 tatccaaacg gcggattgca cgtgtacgtt tttgtccttg tgcagatttc aattcttctt
     3121 tcaattcggc tgcttctgca tctagatcaa cattatcaag taattgttta atcgcttctg
     3181 cacccatagc agcttggaat tcttgtccat attgttcacg tttttcgcga tattcgcgtt
     3241 ctgtcaacaa ttgttttttc tctaaattcg tatttcctgg ttcgatgact acatatgaag
     3301 caaaataaat gatttcttcc aaagcgcgtg ggctcatgtc taatacaaga cccatacgtg
     3361 aaggaatccc tttaaagtac cagatatgtg aaacaggagc tgctaattca atatgtccca
     3421 tgcgttcacg gcgaactttc gaacgagtaa cttccactcc gcatcgatca caaacgattc
     3481 ctttataacg gatacgtttg tattttccgc aggcacattc ccagtctttt gtaggaccaa
     3541 aaatccgctc acagaacaac ccttcgcgtt caggttttaa ggtacgatag ttaatcgtct
     3601 caggtttttt tacttcaccg taagaccagc ttctgatttt ttcgggagaa gccaaaccta
     3661 tttgcatact ttcgaattta tttacatcga tcaaaagggg ttccctccat ttcatttaat
     3721 ggcaaaccgc aagacggctg atcttcgacc gccttgcagc tcatagaccg gattagtcta
     3781 aattatcttc tgcggtttca aagttttgga tgacatcgtc tttttcatct tccacttgtt
     3841 cagcagcttt cttttctaat tctttggcag tttgctgttc ggcaaatttt gtcaaagcat
     3901 caacagtgat caaatcgtcg tcttcatcgt ccatgtcgcg aagttcgatc tctgtttctt
     3961 cgatatccaa gacacgcatg tccagaccta gtgattgcaa ttctttcacc aatacgcgga
     4021 atgattccgg tacacctggt tttggaattg gttcaccctt gacgattgct tcgtatgttt
     4081 tcacacgtcc gactacgtca tctgatttgt atgtcaagat ttcttgtaaa gtataagccg
     4141 caccgtaagc ttccagtgcc catacttcca tttcaccaaa acgttgtcca ccaaattgag
     4201 ctttacctcc caatggttgt tgtgtaacca atgagtaagg tccgattgaa cgtgcatgca
     4261 atttgtcatc gaccatgtga gcaagtttga tcatgtacat cacaccgaca gacacacgtc
     4321 catcaaatgg ttcacctgta cgtccgtcgt acagaacagt tttcgcatcg ctggccatac
     4381 cggcttcacg aactgtttcc catacgtctt catcacttgc accatcaaat actggcgttg
     4441 ctacgtggat acctaactga cgtgcagcca tacccaagtg taattccaat acttgcccga
     4501 tgttcatacg tgaaggcacc cctaaaggat tcagcatgat atcgattggt gttccatcag
     4561 gtaggaatgg catatcttct tccggcataa tacgggatac aaccccttta tttccgtgac
     4621 gaccggccat tttatcccct tcatggattt tacgtttttg aacgatataa acacgaacta
     4681 acatgtttac gcctggtgac aattcatctc cagcttcacg agtaaagatt ttcacatcgt
     4741 gaacgatacc gccgccgcca tgtggtacac gtaatgatgt atcgcggact tcacgagctt
     4801 tttcgccaaa gatcgcatgc aatagacgtt cttctgcaga taattctgtt accccttttg
     4861 gcgtgacttt ccctacaagc aaatcgccat cttggacttc tgcaccaata cggatgatac
     4921 ccatttcgtc taaatctttt aatgcgtctt ccccgacatt agggatttcg cgtgtgattt
     4981 cttcaggtcc taattttgta tcgcgggctt ctgattcata ttcttcaata tggatagaag
     5041 tgtatacgtc atcttttacc aaacgacggc tcatgatgat cgcatcttca tagttgtacc
     5101 cttcccaagt catgaaacca actaaaacgt tttggcctag agccatttcg ccttgttcca
     5161 tagatggtcc gtctgccaga gtatcccctt tttcgacttt ctcacccaaa tgaacgattg
     5221 gacgttggtt gtagcttgtt ccggagtttg aacgacggaa ttttgttaca tcgtatttat
     5281 ctaatgcacc gttgtcgcgg cgtacgcgga tttcagaagc atcaacgtat tcaacgacac
     5341 cgtcatgttt acataacaaa gcagctcctg agtcatgcgc tgatttgtat tccataccag
     5401 taccaaccca tggtgcttga ggattgatca acggcactgc ttgacgctgc atgttggcac
     5461 ccatcaaggc acggttggag tcatcgtttt ccaagaaagg aatacatgct gtcgcaacgg
     5521 caactacttg tttaggtgat acgtccatgt agtctacttt atcaatagat acttctaagt
     5581 tttcactttg agcacgagcc ataacaacgt cttgagcaaa tgttccatct tcattcaatg
     5641 ggctgtttgc ttgggctacg atgtaatggt cttcgatatc agctgtcaag tagtcgattt
     5701 ggtcagtaac acgacctgtt tcacggtcta cacgacgata aggtgtttcg ataaatccga
     5761 atttatttac tttcgcataa ctggacaaac tattgatcaa cccgatgttt gggccttcag
     5821 gagtttcgat cggacacata cggccatagt gggagtagtg aacgtcacga acttcataac
     5881 cggcgcggtc acgagtcaat ccgccaggtc ctaaggctga aagacgacgt ttgtgtgtca
     5941 attcgccaag agggtttgtt tgatccatga actgtgacaa ctgagaagaa ccaaagaatt
     6001 ctttgatact tgccacaact ggacggatat tgatcaattg ttgaggtgtc aatgtttcag
     6061 tatcttggat cgacattctt tcacgaacca cacgttccat acgtgctagc ccgatacgga
     6121 attggttttg taaaagttca ccgactgaac ggatacgacg atttcctaag tggtcgatat
     6181 catcgacatt gccgatacct tccatcaagt tcaagaaata gctcattgaa gcaatgatgt
     6241 cggctggacg aaccgttttt acagctgctt ctggatagcc gtttccgatc acgtttactt
     6301 cacgttcagg gtctcttggt gaaaatactt tgatgacttg gacagtcatt ggatctgtga
     6361 ccacaccgtc ttctgatgga tagtaagtca ctgaatttag gccattatca atgaaaggag
     6421 ctagtgtttc catgacttgg tgagtcagaa ctgttccttt ttcaacgatg atctcgcctg
     6481 tttctggatc aactaatgtt tcagctaatg tcagattcaa tagacgtgtt ttcaaatcta
     6541 attttttgtt tactttgtaa cgaccaacgt ttgctaagtc gtaacgtttt ggatcgaaga
     6601 aacgagcatt cagcaagtta cgtgagctgt ctgctgtttt tggttcgcct ggacgcaaac
     6661 gttcgtaaac gtctttcaaa ccttcttctg ttcttgagtc gcttgcgttt ttatgcaagt
     6721 ctttttcgac tgtattgcgt aatgtttcgc tgtcgccgaa gatttcaaag atcgtatcat
     6781 cggaaccaaa tcctaacgca cgaactaata cggttaaagg aatcttacgt gtacggtcga
     6841 tacgtacata agagatgtct tttgcatcag tttccatttc tagccaagct ccacggttag
     6901 ggataacagt agaaccgaat ccttcttttc cgtttttgtc gacttttcca tggaaataga
     6961 cacctggtga acggactaac tgagaaacga ttactcgctc tgccccgttg atgatgaatg
     7021 ttccttgttc tgtcattaat gggaaatcac cgaagaatac ttcttgtgct ttgatttcgc
     7081 cagtctcgcg gtttgtcaaa cgcaatgtta catgtaatgg tgcagagtag tttgcatcat
     7141 gcgcacgagc ttctgctact gtatactttg gttctttcaa ttcataatct acaaattcca
     7201 aagatagatt gccattgaag tcatcgattg gcaaaatgtc ttcaaacatt tctctaagtc
     7261 cctcatctaa gaaccattgg taagagtctg tttgaatttc gatcaaattt ggtaattcca
     7321 acacttcact gattcttgcg aaacttctac gttcgcgatg ttttccgtat tttactacgt
     7381 gtccagccaa gctcttcacc cctctaaaat gtcattcaaa aagactaccc gatcaattgt
     7441 tacaataata tttcaaatat tatttttagg taacaacaga cattttttgc ggcttttagg
     7501 caaaaaaaaa ccaaaaatag aacattctct tgaaacctta cttcgtgagc ttctactttt
     7561 gtttccttat attaaagccg aaacggacaa tatttcgttt ctgccttttc ttcatcagat
     7621 agtttttgca acttcatata ctacacttat tttctataaa agtcaacact tcctggcgtt
     7681 tcttctgctt tttcgcaaca ttttcttaat gttttcaatt ttatgttttc aattatctga
     7741 caattcttaa ttgacagacc tttttatctc ggttatactt tagaaaattc attttgtgga
     7801 ggaacttcta tgcgaaccaa catcaaccaa atcgcaccaa cacaactgaa taacttttac
     7861 tttagctact ttagataggt ttgtattcat catgatggat acaaacgtta aacgacagtt
     7921 tgtatctatg atggctaaag aactttgtat tgcagcaaaa agccacgtag acaaaatgct
     7981 aagtggctga gcagtgaaaa tacaaagtaa aagtatcaag cattctactt tttcaattta
     8041 ttttccgact tttcaatcca tttggacatt ttgtctgaat ggattttttg tttcccaaaa
     8101 ttaaggagga aaaagaaatg aaaaaaaata taatcatcgg attaggcgct gccctgctac
     8161 tagctttagg aggttgcgca caaaaagaca attccgcaga ggcatcagat ggacaaaaaa
     8221 caattggcgt attgcaactt gtacaacata actcgttaga tgctgcctat aaaggattca
     8281 aagaaggatt agcagaagaa gggtataaag aaggcgataa tctaacaatc gattatcaaa
     8341 atgcccaaaa caatcaagac aacctgaaaa gtatgagtga gaaactcgtc aaagaagatc
     8401 ctgatttgct acttggaatc gccactccag cagcacaatc tttattaaat gaaacaacca
     8461 ctatcccgat gactgtcaca gcggtcacag acttggaaca agcccattta gtcacatcca
     8521 atgaaaaacc tggcggcaat gtgactggca cgacagatat ggtgccaatc gaaaaacaaa
     8581 tcggcttact gttaaatatc gtgccagacg ccaaaacaat cggcatcatg tataacaacg
     8641 gtgaagcaaa ctctaagatc caagctgatt tggcagaaaa agaattgaaa aaagcgggcg
     8701 tcaaagtcaa agtactgaca gcaaattcca caaatgatgt ccaacaagtg acgaaaagtc
     8761 tagctaaaga tgtagatgga atctatattc ctactgataa cacgttcgca agtgcggcaa
     8821 gtgttgtcgg tgaagtcgca aaagaaacaa aaacacctgt cgttgctggc gcagttgatc
     8881 aagtcaaaga cggcggtcta gcaacagtag gaatcgacta cgaaagcttg ggtaaacaaa
     8941 cagggatcat ggcagcaaaa atcttagacg gcaagaatcc aagtgaactt cctgtcgaaa
     9001 cagcgaatga tttggaactg tatgtcaata aagacatggc caaagcatta ggcatcaatc
     9061 cagacagcat caaagaagct gaataaaaca caggaggatt ctcatgacaa atttgctctc
     9121 tcttttctta tcatctgcat cgcaaggggt cttatgggcc ctacttgcga ttggtgtata
     9181 cgtcactttc cgtatcttgg acattgccga cttgactgct gaaggaagct ttcctttagg
     9241 tgcagggatc gcggcagtaa gtattacaaa tggctatcat ccattgacag cttgtcttct
     9301 aggcttttta gggggtgcgg cagcagggct tgtttccgga ctgcttcaca caaaattgaa
     9361 aattcccgcc cttcttgcag ggatcatcac catgactggt ttatattctg tcacatcccg
     9421 tgtgatgggt gcgccgaaca tctctttatt gggtcagcca acgatctttc attgggtcga
     9481 gtcattcggc ataacaaaag acaacgctgt actgatcgtc ggtctgcttt ttgctttgat
     9541 cgttgtatgt ttacttgttt tgttcttacg tacagaaaca ggacttgcta ttcgtgcaac
     9601 tggcgataat ctggcaatga gcgaagcaaa cgggatcaac acagataata tgaagatcat
     9661 cggttatatg atttctaatg gatgcattgc tctttctggc tccttgcttg cgcaaaacaa
     9721 cggctttgct gatttaaatt ctggtattgg aacgatcgta attggacttg cttctattat
     9781 catcgcagaa gttttgttac gtaaccagcc actatttctc cgtctcttga ctgtcgtttt
     9841 aggtgcggtc atttatcgtt ttattttagc gttagtcttc gaactgaatg ttgaaccaag
     9901 tgattcgaaa cttgcttctg ctttcgtgct agtgatctgt ctttctttcc cgcagatcca
     9961 gcaaaaattc catttacctc aaaaaaagaa aggcggtagt gtcaaatgaa tgacgtctta
    10021 acattggaaa atctccatca tacctttgaa aaaggaacga tcaacgaaaa tcatgtcctg
    10081 aaaggcatta atctttcctt agatcccggc gacttcgtga cgatcatcgg tggaaatggg
    10141 gctggtaaat ctactttgct gaacagcatc gccggtacat tttctgtcga tcagggtcaa
    10201 attttattga atggaaaaaa tatcacgaaa aaatctgttg tcgaacgttc gaaatccatc
    10261 agtcgcgtat tccaagatcc taaactaggg acagccgttc gtttaacggt agaagaaaat
    10321 cttgctcttg ctatgaaacg aggaaaaaaa cgcggcttct tccgtggtgt caaaccacaa
    10381 gaccgaacat ttttcaaaga ccagctggtt cggctaaatt tggggttgga aaatcgttta
    10441 tcctcagaaa tcggtttgct gtctggagga caacgtcaag ctattacgtt attgatggcc
    10501 acattacagc gtccagaact gatcttatta gatgaacaca ctgcagcgct tgaccctgct
    10561 acttccaaaa cggtgatgga attgacagaa aagctgatca ctgagcagaa tctcactgct
    10621 tttatggtca ctcatgacat ggaagacgcg atccgctatg ggagtcgact gatcatgctg
    10681 catcaaggaa ggatcgtcgt agatatccag caagaggaaa aacagcagct cacagtgccc
    10741 caattattag cgttgttcaa acaaaacagc ggcacggcat taaaagacga tcaagtactt
    10801 ttatcttcat aatcagaaag aggatgggac aaaaaatgaa cgcccaccct cttctttttt
    10861 ctctttcctt ctttcgtttt tgctttatgt atcaaccgtt gtttcttcga aaactatttt
    10921 gttctctcct tcttcctgat agaatggatg ggaaaggagc ttgatgccat gacaacgaaa
    10981 gacaaggtac ttcaattatt gaaaaagaca ccagattttc tttccggaga aaagttggca
    11041 cagcaactgg aagtttcccg gacaagtatt tggaaagcca tcaaagaatt ggaaaaggca
    11101 ggctatcgtt ttgagcacgg gcctacaggg tatcgttacc tgccctctga cgtactagat
    11161 gcaaaagaaa tccagcaaga gctacggtcg tttattcctg atttgaaagt acaaacgatg
    11221 ttgacttctg aatctacgat gaaagatgtc aaactggcag cagctcaagg agattctgtt
    11281 tcgaagttat ttgttgctga tacacaagaa caagctcgtg gacgttttgg acggcctttt
    11341 ttcgctgaac ctggacgagg aatctacatg agtctcttgc tccatccaga caaacggttt
    11401 gatgaattgc ctcaatatac gatcatcgca gccagtgcct gtgtgaaagc cattgaatct
    11461 gtaaccggtc gttctgcttc gatcaaatgg gtcaatgata tctacatgga tggaaaaaaa
    11521 gtctgtggga ttttatctga agcagtcagt gatatggaaa gcggcaggat cagtcatatc
    11581 atcattggga tcgggcttaa tttttctgtt cctcaaaatc attttccagc tgatctccaa
    11641 gaaaaagcca tctctgtctt tcccgacgga caagctagtg tcacaaggaa tcagctaatc
    11701 agcaaaatct ggcaagaatt gttcacttat ctttctcacc cagaaaaagc gttggacttc
    11761 tataaagaaa aatcattcgt attgggaaaa accgtgtcgt tcaatcaaaa aggaacgact
    11821 tatgtcggtc ttgcaaaaga aatcggtgat catggcgaac tgatcgtcca aacaaatgaa
    11881 cagaagatga gtttgtcttc tggcgaaatc agtctcacat ctatccgtac gcaataatta
    11941 tcagatttca caaaaggaca cctgatctca tacagtgtcc ttttttctga attctcttca
    12001 aaagactctc cttattctat cagctgacag tttctctatc ctctagaaaa aggtattcta
    12061 aaaatatcta taaagcatgg tacactttct ttaaatgaat caatgcaaaa aacaagagag
    12121 tcatcagttt acacgatcaa aagagatagc aggccgcttc ctttttcaat cttatagata
    12181 gaccatgggt aacgataaaa acaaaaaaga acttgcgact tattttgccg taagttcttt
    12241 tttagctagt gccttaatct cttaatccca acaaacgtct cgcatagatc acctgaccag
    12301 agtgcatagc agcatcatca ataatagaaa cgagacgcat tcctcgtgtg actggtggtg
    12361 tccagttctc gtcaattact tcatctagct cggaaggttc cagccttctt aagtaagaaa
    12421 tagcaaaatc agcagctcct cgcaagtatc ctagcagatc ttctatttga ttagcttgga
    12481 tctgctttgc ttgttccaga ctatgcttcc aatcttgttc gttatctgct atcgtcatcg
    12541 gaaaccggct ttcccagtct tgactatgcc aaattggaga agttcctgct aaatcagcaa
    12601 tctggaaatc taattctcga gcggtatgcc aagccagcca agtcattgat ttgatctgcg
    12661 gggctctatc ttccactgga aaacgattgg cctcctcaat agagacgcct tcaaacaaac
    12721 gttcaaaccg atcgtatgcc cgatccacac tctcgcatag catatctaaa taatccattt
    12781 attttcctcc tttttattta tcctacttac tttttcctaa ttctaggata cctacttata
    12841 tgttactaca cacccttatt ttttacactt taacagtcac catcatcaat agtttggaga
    12901 ttgaaagaaa aagatagtgt cgaactatca attgaacaag aagcaacaat atgaacgttt
    12961 cattaatatt ctttaaataa acaatgattt ttcaaacgaa cagtttaaga ctaggagaaa
    13021 aagagtataa taggaaaatg tttgaaagga aaggaagggg aaaaatgaaa aaaagggcaa
    13081 gtttactagt cctagttgct ttgttgttca atatcttttc gtatggtgtg gccgtttcag
    13141 cggaaacata cattcctaaa gaccctggga atatttataa tgatctaact ggtaactatg
    13201 gcttgttagg aattgctagt caattccacg tatttgctaa aaataagaca actgtgaatg
    13261 cacatacaga cggaaatata gcaacacatg aacttgatgc aaaaaataat tttggtacag
    13321 agcttcatga aggtttattg aaccaagaaa taaattatat acaaaaaact gattctttcg
    13381 ctaattcttc aggcatacct acaggtagca caagaatgaa taaatttgtc gttggcagta
    13441 acgtggatgt agcacttcaa gatggtcatc ctacagtcaa tggatccagg cttgatcact
    13501 taactccaga tgatttttat aaagatcgtg ctgggcatga atatattgat ttcgagcagg
    13561 aatttgctaa attgaatgta gcagcaaatg atcttgcaac gatcactcca gctaaaacat
    13621 atactgctgc agattttcca gacatgaaca atcggacaat cgatttaact gatttatcag
    13681 ataccgggtt tttattggta aatatcgatg cagaagtgtt aacgatgaat acgccacttc
    13741 aaatcattaa tccaaatgat caagtagttg tatttaatgt catcaacagt tcttctgcat
    13801 tgaacgtaca gtcaccaatc aaatacaatg atcgttcaaa tcatgaaaca gaagattttt
    13861 cggatgcgaa tatctcttgg aattttggaa atgagatgac tgatttgaca atcagtgctc
    13921 cttttcaagg aacaatcctt gcgcctaatg ctactatccg cattactcaa aaccaagacg
    13981 gaacgattat aggaacaaat gtgattttag atgcagctac caatcgttgg gatcctaacg
    14041 aaatatttat cagcgacaat ggtacagata cgacagacac aagcgacagt acagatacat
    14101 ctaccacaag tgatagctca gattcatcaa cttcaagcga cagtacagat actacctcta
    14161 ccacaagtga tagcacggat acatcagctt caagcgacag tacagatact acctctacca
    14221 caagtgatag cacggacaca tcagcttcaa gtgacagtac agatactacc tcta
//
LOCUS       DADVXU010000070        14221 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.073, whole genome shotgun sequence.
ACCESSION   DADVXU010000070 DADVXU010000000
VERSION     DADVXU010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.073"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            476..1339
                     /locus_tag="KB953_001976"
     CDS             476..1339
                     /locus_tag="KB953_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBB6866948.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="KB953_001977"
     CDS             complement(1468..3090)
                     /locus_tag="KB953_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBB6866949.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="KB953_001978"
     CDS             3339..3860
                     /locus_tag="KB953_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBB6866950.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="KB953_001979"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="KB953_001979"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HBB6866951.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="KB953_001980"
     CDS             5941..6759
                     /locus_tag="KB953_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HBB6866952.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="KB953_001981"
     CDS             complement(6862..7059)
                     /locus_tag="KB953_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866953.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="KB953_001982"
     CDS             7228..7806
                     /locus_tag="KB953_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HBB6866954.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="KB953_001983"
     CDS             7901..8038
                     /locus_tag="KB953_001983"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866955.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(8851..9354)
                     /locus_tag="KB953_001984"
     CDS             complement(8851..9354)
                     /locus_tag="KB953_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBB6866956.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9713..10738
                     /locus_tag="KB953_001985"
     CDS             9713..10738
                     /locus_tag="KB953_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBB6866957.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            11061..11939
                     /locus_tag="KB953_001986"
                     /pseudo
     CDS             11061..11939
                     /locus_tag="KB953_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
     gene            12415..12531
                     /locus_tag="KB953_001987"
     CDS             12415..12531
                     /locus_tag="KB953_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HBB6866958.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13459..13797
                     /locus_tag="KB953_001988"
     CDS             13459..13797
                     /locus_tag="KB953_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866959.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13891..14121
                     /locus_tag="KB953_001989"
     CDS             13891..14121
                     /locus_tag="KB953_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866960.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
BASE COUNT         4516 a         2375 c         2720 g         4610 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga ataaatatta
     8281 acgtgttttc aaacagtata aaacggtgtg gtttttggaa taaaaaaata acatatctca
     8341 ttttacactt ttgttatttt atacttaaaa aataacgaca cagaaaatac cgtgtctcag
     8401 atttctctac tacttctaaa aactactgtt tttttccttt gaaatgcctt caaaaagtag
     8461 gatcaggcag aatatactgg cagaatcaag gagaagggag cctaaataag gagaaaaacc
     8521 atagaaaata taaagtaatt acttataaaa caagcttttt atagaaaaat aatatacctg
     8581 agtagggtat aaattaagac ggaattcctt tctagattga gatattgact aggaagaatg
     8641 ttaagatttt aagcctttaa gctaaaaaaa aacccttttt gttaaataag gggaatatag
     8701 cctgtcagtt tcaaactgca agtcgtcctg ctgcaataat atagtagatt agagatgaaa
     8761 atagattttt ttaaaccaat gactgaataa aaagaccgtc atagatacac attggaatgt
     8821 gtctattacg gtcttttttt acgaacgatt ttattgaaga cggacagttc gggtcaaaaa
     8881 taaaaaagct acgatcagaa ataagaataa aatgtatccc aaaataatgg ctagacggtc
     8941 taacttattt gtcgatagat gtttgtttag aagttgatag gcccataaac ctatcagtcc
     9001 tcctgttgta ttagttatca aatcagtgat gtccgctacg ccaataccga ataggaattg
     9061 aatcaattct gccaagaagc ttacaaggaa aataatccct aattttttag aaaaagaaag
     9121 tctcttgaaa ttgacattca aaagcacccc aaaaggaaaa aagacaatca agttataagt
     9181 ggtctcacgt aataccgtcg tattttcaaa agaaaaagta gaaaaaggga cgagattgac
     9241 attgctgtaa gaataagtga gaaattgtgg aagattaaat gatagcttga ataaaatcaa
     9301 ccataataga aagaataaat aaatccctaa taaaaatttt gatacatacc gcatagacat
     9361 ctcctccatt taaaaagtat accatcgaaa accgctgaaa aatcataaaa aagtatgaag
     9421 tttaaggacc aacgtagtta tcgtacgaaa attagaagct tttttttgtt gggaatccgg
     9481 tttctttaac aaagctaaga tagcgagttg cttggaaaag ttgctatctt ttttctgtca
     9541 aaacgactcg gaatgttcgt tttttgttct aatttctcca agttgtttac tttttgatac
     9601 taagtttatg aaaaatcaag aaatagttgt tatactaggt aatgctgaca aacgggattg
     9661 gcatttcatc ttggttcagc tttttttcca attactagga aggagggata gattgttcaa
     9721 gacttatttt cgtttcaata aaatacaaga taatcctttt cgtatcatga atctgatgct
     9781 acttatcttt attgtattat ggttaggtta cataaatcat gattttttga tttcaagttt
     9841 ttcatcatta gggatcttta cttttttcta ttatcaaaac ataccgatga aatatttgct
     9901 gaaacgtatg tttctgatcg gttgcggact gttggttgct ttcgttttgg ggatcttatc
     9961 gacttatgtt ttatggcttg aaccatttgc cgtagctttg gttgcttttt ccagcagatt
    10021 cgtgttgcga ttattccata tttctaaacc aggtggcttg ttttttgcga tgctttctgc
    10081 catgggaacg agcatgcagc tgccaatcgc tcaattaccg atcgtctcac tttatttttt
    10141 tatgggtgtc gtttttgccc tgattgcggc agtgattaca aaattactgg attcacggcc
    10201 tgagcagaca attgaaaaag caactttaaa agaaagattt cacgaagagc ctttagtgat
    10261 cattgattct gttttctaca gcgctgcctt atttctttcc gtttatgtca gtcacggctt
    10321 gaatctgcat aatccttatt ggttgacctt gtcatgtgca tcgattttgc tagcagaaaa
    10381 cttggatgcg atgaagcatc gacaagtcca atatctgatt ggatcgatgg gtgggttatg
    10441 tgtgtcggca ttcttgtcat ttgtgccgtt tacgcagttg cagaccattt ttctgatcac
    10501 ttttctatat ggaatcgcac aatttctagt tgctagaaat tatgcagttg ctaatatttt
    10561 tcttaatccg atggcgttga tgctatctac acttattcgt ggagcatact tgatttcttt
    10621 gatcgaatat cgttttttag ggatcgtcat aggaagtttc atcggtttag gtgttgcgtg
    10681 ggtaatgacg gttggtttgc aacattattt aacggttgtt cgtcaaaaat tagaatagaa
    10741 ggacaaatat atgtgccaaa agaggttatg acaagatttt tgtcacaacc tctaattcaa
    10801 ggtgttcaaa agccagattc tcagcattaa gtcaaattca cacaaaaatg tggaaaggaa
    10861 ctttcaaaaa aattttatat ttattctatc aaaaaatttt gtttatgatg gtaactgaat
    10921 aaactttcct gagatggtca atgctttcct tgaaagtctt acagatattc tttcttcata
    10981 gaaagtttaa gttttatagt tagtgtacaa taaaagtatg tttataccat atggtgtccg
    11041 gataatcagt catttaaaca atgaggttca aagatatgaa aaggggaaat ttgatgaaga
    11101 aaataagcaa tgaaatccaa attctggcag aaactttaac tcttcccaaa gtcgttatag
    11161 agcaaatgat tgtacaagca aaaaacatcc ttgatgaggt gtttgagcaa tatagtcatg
    11221 aactaatgga tcctaataaa gcaaaggcga ctgcaaagaa aatagacaaa gtctatgaag
    11281 aggaaccttt tgcagcatta gctatttatc tatatgcagc ttctcaaagt tggaaaaaaa
    11341 tttatcaacc attgaagatt ccaagaacta tctatttggc aacaatgaat gcttttacta
    11401 gatttattca tgaacatttc caagtaacag gaagctatcg ctttgaccgt ggtttttgga
    11461 tttggcatta cactagtggc ttgatctttc ggattggtga attggagtac gaaatgacat
    11521 attttccgaa agggtacacg gatctaagat tagaaggaaa gcagtgcctt tctcttcata
    11581 ttccttccga tgcagattta tctcagaata agataagaga tagttacgaa agtgccattc
    11641 gtttttttga actttatttt cctgattacc attaccaagt gatgtacaca gatacttggt
    11701 tactatcacc caatcttact aaatagttga aaaaagagtc aaaaatctgt ctttttgcag
    11761 ccgattatcg attattatca gttgatgaac aggacgatag tggagtccct tggatctttg
    11821 gacgagtaga tgcccaaata catgattatc cagaaagtac gagtttacag cgacaagcaa
    11881 aagagcaatt gcttgcaggc gagcacattg gctctggctt aggtattctt acttcataag
    11941 tttagtactt cgaaatagtt taaaagagcg tgagacaaaa gtaaagaaca cttttgtctc
    12001 acgctctaaa cacgtataaa cggcggaagc agaagcaact ccttcggaaa taagctgaaa
    12061 ttcacaaaaa tttgaaaagc aattttcgtg aatttcttct tatttcttgg agttaaacgc
    12121 ttctgtccca gcctcttttt tagtgttcta tcgaacacac agtacgttca ataggtaaac
    12181 tagctataac aatagatatt aaagttaacg gacaagaggg ccttttttag gtagctaaaa
    12241 cgtaataatg aaaatttatt tccagagata ggagtcaatc agctagtctg atcgactgtt
    12301 ttaacaagtt gactttcatt ttaacttttg ataccattta aaacacaaat cgtaattgtt
    12361 tcgatttgtg ttttttagca tgactacaat caaagaagaa agtgggtcgt ccatatggaa
    12421 aaaacagata tctcaagtgc ataccgccgc ttgaaaagcc caaatatcaa aacacgaaaa
    12481 cgtgcgctga agatcatcaa agatgtgaaa cggaatagtg ggaaaaggta agaagaagga
    12541 aacttaatta aaaaaatagc cgaaaaacgt ttttcggcta ttttcaataa tcggtttttg
    12601 tgtaaggttt ccgaaagaac tgctgttgat tttccatttt taacatatga taaattggta
    12661 gaatctttct tttcaaattg ataccattcc ctaattccag ttatatagtt aactacagaa
    12721 ccacaaagca cgtacgttta taaaagtatt atctgtagtt atttttcaat tccctttcaa
    12781 cctcttattt tgaccagtag tattgatcac tttacctttt aacacctttg ttcgtttttg
    12841 aaacattttt agtcaagaat ggaaacgtaa ctacataatc aaaggttttg cgaaatattt
    12901 catgaaatat ttcatgtaga agcgagcaat acggttgcaa ataaccatta tcgtttaata
    12961 agcagttacg aaagctccca atatctctaa cgttcagctc actccatttc ttttcttcaa
    13021 aaaaatcagt gattcgtatc tctttctatt tgtcgaaaaa gttaacttta aaaagtaacg
    13081 ccgaaaaaat atgcaaaaaa ataaaaaagc agttactatt gctagaataa catcaaggaa
    13141 taataagttt tggttttcag ataatagtcg ataagttgtg tattggaatt ttagatacag
    13201 taataaagta tttttcatga aattttgtga gaatcactat tacaagtatc tttaataaaa
    13261 ttaatggtta aggaggaact taaagtagaa tgtaaatgaa tagttgtagt cagtcgattg
    13321 tgatactaga ctcaaagttg caggaataag tttttcaatt tgtgattgga agataaaaaa
    13381 tgaaaaagaa gggctttttt ttatactttg ttgattgtag tgtttatttt ccctctgttt
    13441 agccaagtaa cattttctat gactcaacca gtttcaagtg aggaacatga taaccttttt
    13501 cgtgctacag aagagtttaa gaaatatcag gaaaatattg ttccggagac tccattagct
    13561 agtgagccaa ttactgatat tgatggaaac caaataggat ggtatataca gtacgaagtt
    13621 atggtggtaa ataatgactc aatagtaaaa aatacagtag gcttagagct aacaccgatt
    13681 gaaaaggtga gttcattgtt aacgtatatt tatactgatg aaagtgacga gactgagatc
    13741 attttaacgg attactcaaa aattgctact gataaaaagt atatattgtg gatgtagaca
    13801 ctagtgaaat actttatgat atagaaattg atgaaaattt actagatgta gcacaagaaa
    13861 tcgatcatca aaagcaggaa gctattcgat atgctggtag aaaagggaaa agaaaatatt
    13921 tattacgtga atgtagcaaa agttagagaa gatgaaaatg agtggaaaga attcaagagt
    13981 cgctactcaa ttaactcgac tccgacattt acagtttaca gagaaggatc gatagaaaaa
    14041 actgtttttt ggactaaaga aagtggaatg tctttagctg aagtagaaga gtttttggat
    14101 tatgttagta tgcaacagta agggagttct tattatgaaa aattttgaca catattatgt
    14161 tattgttttt tcgatatctt tgctgttaat attactaact ttaatgcagt ataaagagta
    14221 t
//
LOCUS       DADVXU010000071        14130 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.074, whole genome shotgun sequence.
ACCESSION   DADVXU010000071 DADVXU010000000
VERSION     DADVXU010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.074"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..244)
                     /locus_tag="KB953_001990"
     CDS             complement(<1..244)
                     /locus_tag="KB953_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBB6866961.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYNELI"
     gene            complement(237..1829)
                     /locus_tag="KB953_001991"
     CDS             complement(237..1829)
                     /locus_tag="KB953_001991"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HBB6866962.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLANFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHE"
     gene            complement(2339..3064)
                     /locus_tag="KB953_001992"
     CDS             complement(2339..3064)
                     /locus_tag="KB953_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866963.1"
                     /translation="MKKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNII
                     EEIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAI
                     DNVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPP
                     TWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKK
                     DYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(3218..3544)
                     /locus_tag="KB953_001993"
     CDS             complement(3218..3544)
                     /locus_tag="KB953_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866964.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(3581..3946)
                     /locus_tag="KB953_001994"
     CDS             complement(3581..3946)
                     /locus_tag="KB953_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBB6866965.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(3983..4198)
                     /locus_tag="KB953_001995"
     CDS             complement(3983..4198)
                     /locus_tag="KB953_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6866966.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            4327..4662
                     /locus_tag="KB953_001996"
     CDS             4327..4662
                     /locus_tag="KB953_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6866967.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(4738..6735)
                     /locus_tag="KB953_001997"
     CDS             complement(4738..6735)
                     /locus_tag="KB953_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866968.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVRDNSVKNALIENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPIYQFTSVTRDDGRRVRNRRDPIILN"
     gene            complement(6833..7108)
                     /locus_tag="KB953_001998"
     CDS             complement(6833..7108)
                     /locus_tag="KB953_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6866969.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            7340..8074
                     /locus_tag="KB953_001999"
     CDS             7340..8074
                     /locus_tag="KB953_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6866970.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            8113..9255
                     /locus_tag="KB953_002000"
     CDS             8113..9255
                     /locus_tag="KB953_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBB6866971.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(9883..10368)
                     /locus_tag="KB953_002001"
     CDS             complement(9883..10368)
                     /locus_tag="KB953_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HBB6866972.1"
                     /translation="MVRRVFFSFHYADVTRAMVVRNSWTLRGTKEAGFVDKAEFEKVK
                     RNGDIAIKNWIDNQLIGTSVTVVLIGKETLDREYVKYEIQESYKRGNAILAVNIGKIK
                     DFNQMTTTSQSVIKIIGQKPDGEYLWFDEIIDGQYDYISDDGYNNLGTWIDSAAYKKG
                     K"
     gene            complement(10370..11134)
                     /locus_tag="KB953_002002"
                     /pseudo
     CDS             complement(10370..11134)
                     /locus_tag="KB953_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287699.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(11304..12497)
                     /locus_tag="KB953_002003"
     CDS             complement(11304..12497)
                     /locus_tag="KB953_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBB6866973.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            complement(12581..12781)
                     /locus_tag="KB953_002004"
     CDS             complement(12581..12781)
                     /locus_tag="KB953_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBB6866974.1"
                     /translation="MNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVIM
                     NGNRIQIKRKQFEKIIDTLDTI"
     gene            complement(13205..13438)
                     /locus_tag="KB953_002005"
     CDS             complement(13205..13438)
                     /locus_tag="KB953_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6866975.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(13445..13876)
                     /locus_tag="KB953_002006"
     CDS             complement(13445..13876)
                     /locus_tag="KB953_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBB6866976.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
BASE COUNT         4295 a         2895 c         2270 g         4670 t
ORIGIN      
        1 tgattaattc attatatttt ttttcagtaa ttttatatct tgtttcgtaa ttatcataaa
       61 tagcattttg ttgttcatag acaacaaaat tgctattaga tacaaagaat tctttcttta
      121 aggcactccc aaatggtccc ctccttactc catctttttt atcaagtaag cattcaaact
      181 tacgctgttc ccaagcttcc gtaaatcctg ggaaacgaat ttctggttgt atatcattac
      241 tcatgtttga acacctccag cgcaccttgt aaccacgctt cattttcagg tgttgtttgc
      301 agggaagaga tagcttctaa aagttctttt tctagcactt gctgttcttg tcgtatcttc
      361 ttgatgtcat ttccgacatg aaccatatca attggttctt cttcttcaaa cgtatctaca
      421 tagcgtggaa tatttaagtt atagtcattt tctttgattt catcaaagtt tgccaaatga
      481 gcgtatttct cgacatcttt tctttcagca taggtttcga ggattttttg aatgttttct
      541 tccgataatt tattctgatt ttttcctttg acaaattctc gactagcgtc aatgaataat
      601 acgtctcgag tttggcggtt ctttttcaat acaatcaccg tcgttggaat cgatgtcccg
      661 aagaacaagt tcgcaggcat accgatcaca gcataaatac tgccgtcttc tagtaatttt
      721 tgacgaatga ccccttctgc agcgccacgg aataaaactc cgtgtggcaa gacgatggcc
      781 atagtccctg tttccttcaa atgatagaaa ccatgaagaa gaaacgcaaa atctgctttg
      841 gattttggcg ctaattttcc ataacgatta aaccgagaat cgtctaagaa ggtcgtatct
      901 gctgaccaat tagccgaata aggtgggttc attactactg catcaaatgt gtaaggttca
      961 tctgttggcc aatccttatt caacgtatcc ccattgcgga gattcatttc ttcggcatct
     1021 accccatgta aaatcaagtt catttttgct agattgtatg tcgttgtatt taattcttgc
     1081 ccatgatatt taacgttatc tgggtgagtt aagtagttac gcacatttaa catcaatgaa
     1141 cctgaaccca tggttggatc aaaaacacta aagaatcgtc gttctttttg atccaacgtc
     1201 acgatttgtg ccatcatatc agaaaccata tgaggcgtgt aaaattctcc ggctttcttc
     1261 cctgcttctg aagcaaattg actgatcaga aattcataag catccccgat cacgtcccca
     1321 tcgtgaccta aaacttccac gtcattcaat ttcttaatga cttcggtaat cgtcacattt
     1381 cgttgttgct catctgttcc tagttttttt gattggagat ccacatcatc aaataaccca
     1441 ttaaattgat tgtaggtaga tgccaattgc acaaaggctt tgtttagatc attcaattgg
     1501 aacgttgctt gttttgcttg atccgctaag acgttgaaaa gatactctgg cgaaatcgca
     1561 taccccaaaa tatcaacgat tgttgcaatc agatcttctt ttgattcttc atctgaaagt
     1621 aattcgcgat acaacatcgt ttgtttgctt accgtatcat attcctctaa ggattcatct
     1681 gctaaaagaa ccacttgttc caacaaacgg tcggataaat atttatagaa aatcagtccc
     1741 aataaataat ttttatattc tgacgcatcc attttgctac ggagattatc tgccgcacta
     1801 aataattttt gatttaattc tgcacccaaa aagatacact cctatctctt atttaaagtt
     1861 gctatatacc ctcttatttt agcatactcc cttttagaaa aagcaaaaaa aaaaagaaag
     1921 aaacttattc ttgctaataa actacgcgaa aggagtctaa cgttgtttga tataacttag
     1981 actgatcgtt aacttatttt ttatatttct acatagttat ctttagaacc atttcagata
     2041 catatgaaac aattacatat gattttttca taaaaaaaca gtactgtcta gtagtataaa
     2101 atatacaaat ttcttaaaaa ttttttcact aaaatcacta cctgaaacag tctaaaacac
     2161 ttatatatca acgtttaatg aaactatctc cgtttcattt cagatacaaa taaatatagt
     2221 cgggctccaa atccgaaaaa aaataccccc agtagcttat cctaagctac tggagatgta
     2281 taagtacggt catataacaa cggggtaggg taaataaatg aaaaagatgg aagaatcatt
     2341 attcaataac ttttttaaat acttcgttgt aatcagttac ctgtccgttc ttaaatacca
     2401 cagaataatc ttttttccca ttttcaagta accgaagtgg aatatcttta ccaactttct
     2461 ctgtcactgc attactgata cctgttagat tagctaataa atctgataaa gattgagcat
     2521 ctgtagaaat ctctgtatca gtatttatcg tgattccttt atcttcattt acaagtttcc
     2581 aagtaggtgg tatagctgtg tatgtttcta caaatgaatt aagtttttca ttaagtgact
     2641 tctcttttat atttccatct aaaaactctg tatatatggc ttgagctata ctaaaacaaa
     2701 gctggccttg ttctaataat tttacatcat tagttagtaa agctgtgttc acgttatcaa
     2761 tcgcttcaag aagagtatct ttggtgtccc taacagcttt aggtgtgcct tctggaattt
     2821 ccctacttga tacctcgtcc attttatctt ccataaaccc cttaaaatca tatctttctc
     2881 ccaaatccat agaagttttt gagaaagctt tcatttctga gtctatttct tcaataatat
     2941 ttaacatgtc tgatacatat tgtttaggct gattcgtttt taatctatat gtggtaaata
     3001 tctcaattcc tccaattatc aataaaattg ccaaacaaat ccataataat tttttctttt
     3061 tcatttttcc tcctatacac tgctgttata acagtgacta atgtaaagat tttcccagca
     3121 atcatcagac tattatttac agggaatcaa aaaaactcct tagtaagctt acacctttag
     3181 gagttcctgt ttgaacggta tgcataatac atttaaatca atttaacatt aacacctcag
     3241 tatatggtat ctctacatat acacggtctt tttttggata gtactcgaca tttatattga
     3301 tagcacttcc attgtctaac gtagctttag cccggtacgt gttatcacca ttatcatgag
     3361 tgatcgtcac atcatcacat gtccgattta agttatactg ttcttgtaaa atctgagtta
     3421 ccaatggtac agatgacttc tctagtccag atgaacccgt cattcttgaa aaaattatac
     3481 ctataatagc caatacaacg atacctataa tgacaaactt cttattcata tttactttat
     3541 tcaacttttt atctcctatc tccaaaatga ttttctctca tcagtaactc attgacatcc
     3601 ccaatagtac accaaagatg gctccagcta cccctgctat catcaaaatc gtacccattt
     3661 gttcatcttt ctctcgatat aagtaaccca taataacagc aacagcacca aataccacag
     3721 gaataaatgc taatgatact accatagaaa tccaaccaat aatcaaatac agcttccctg
     3781 tctcttgtcc atgtacgatt tgttccttca tttcattaat tgtctgctca gtacttttct
     3841 ttactgtgtc tgttccggtt gtctcatctg tttcttgtaa tgctccgcaa aactggcaat
     3901 acttactttc atttgttatt tctttaccac attttctaca atacatgtct tctcctccta
     3961 aaatcattca attgcttatt agctaactgt ttactttatt aaatacatca gaaaacagct
     4021 catttattgg tacacttaat atctcagaaa gcattttcat ttgatagggt ttaggaatcg
     4081 ttctccctgt ttgccacgaa cttacacatt gctgtgtaac attcattttt ttggctaact
     4141 cagtttggga aagtcctttt tcttttctgt atcggctgag attacttact tttttcatga
     4201 ttctcccctc ctgttttatt atatacaaca atatgatgta tttcaactca taaaatatat
     4261 aaatacttca aatgtcatta caaatacaac aacttgttgt actctactga gaaggggggt
     4321 gtattaatga aacagaagca atatgcaaag gtcattagag aaaagcgaaa agcactcggg
     4381 tggacacaaa aagaattagc taaaaaaatc ttctctacac aacaggcagt agcaagatgg
     4441 gaaaattctg taacagaacc taacctcgat agcttaacag ctctttctag agccctagga
     4501 acccctgtaa gccattttct agacaatgtt gacatagatt atgaggaaga gtttttggct
     4561 ctctatcgct ctcttagtac tgaagatgca attcgaacaa ttgactatat gaagctatta
     4621 aaaaggcaag aaaatgaacg caatcagcta ttgaaagatt gaaaaataaa taacccaccg
     4681 aactttaacg gtgggtgtat tgagatgaaa taagattaag agctatggat aacctgtcta
     4741 gtttaatata attgggtctc ttctattcct tactctccgt ccatcatctc ttgttacact
     4801 tgtaaattga taaataggaa ctaattcaac tcctgataat atcaattctt ttattacttc
     4861 atctatccct gcccctttcg ctagatatac taaaaaacta ctgcttgctt tcacattgta
     4921 tagtttctta atatccttag aagctcgttc aagagtgaat ccttgtttaa caaagtagcc
     4981 tctgttttct attaaagcat ttttcacgct gttatccctg acatttcgta ggcgttcaca
     5041 aacagtgtta ataagttctt tttctgtcat tagatatgtc ccttgccacg ataagccctg
     5101 taaaacttca tgtgcttcat ctaaaatcaa ttgtttttcc ttgctaagtt ctgatcggga
     5161 attttgagga aatactcttt tagcctcttt ttcgttaaaa catatcttaa cccattcata
     5221 agaaaaatat tgatatgtaa aatgaaattt ttccagaagt ggaaggatat cttcattcaa
     5281 tttttgaatc acagaagttg cattaaaaac gtctaatcga tagacattgc ttctcttatg
     5341 tttaaagttt cctaacgctt tctcttcctt taacctattt aggtattcga gcatcttcaa
     5401 aggaacatct tcatcatcta atttttttat caatcctata aaagtcataa gtgatagtaa
     5461 cttattacat ttatctatgt cataagtttt accatagtaa gaactcattt ttttagctaa
     5521 ttgttccccc gacaaaatac aagtcacact ttccttacta tcatcaaaaa aattaacatc
     5581 agaaagcaag acatcataga cacgtcgcat ttcctctaga taattcctta ttgtcctgaa
     5641 aagtttgtct ttcattgatt caagagtgtc aaaaagtact tttgattcct ctttcaattt
     5701 tcgaaaatgt gtaggataca agtctaggtt ctctaatagc caattaaatg tttctacccg
     5761 actgtctttc tttaaaatta gcatcacaac tccaataaaa ttttcactga tatcacaatt
     5821 actggaatga caataaaaaa tggaatgccc agttttactg gtaaaaatac tagcagaggg
     5881 attatgatcg ttgtgaaaca gacatcttaa gtttttactt gacttaagta atttattcat
     5941 atcaatggtt aacaatcttt cataaatctc attaaaagac atgtcggagg aatcaaacag
     6001 gcagtcttta aaccactgtt tcatttcttc tttgtcgtca ttttttatca acttaacaaa
     6061 atcggtttca ttgactagtt catactctct ccctgaatca ctttgacaat ttttagaaca
     6121 tgtcatggta aaatcaatcg gaagctcagt aatatctaac atgttgtctt cattaattat
     6181 tatcactgca tctgacttat atcctccaaa aaacattcta ttcgaagcat ttacattgtc
     6241 atcacacccg gggatgttct ttttcaagta ttcgtaaaca gcaaccattt taccatagtc
     6301 agtaattgct tcttttaata gaaagacaat cttgtatttt tctagatttt cagagtaccg
     6361 aatacttttt tgtatcgcaa acgcagaatt atgaactaaa ttgatagata aaaccttttc
     6421 tatgcttaag tatctactat catctttact caaatctact accttcccct tctcatcttt
     6481 aacagtgtta tcgacatcca gaataattac tctggtttcc acaaaactct ccttgtcatt
     6541 gcctttattc tttgtcttgc ttggaataaa tgattgtgat gaatttagta tctctacaaa
     6601 ctcatctctt gtaacttctc tatagttatc taaacaccta tctcttacat cagttttttc
     6661 tttttttgta actggcttct ctaataattt ttctttatct acataaattg gaatattaat
     6721 cattgatgtt tccatttgtg tttcctcctt ataaaattct gttaaagact aaaacataca
     6781 gataataaaa acaaactctt tattactata tgttttagtc ttatttgttt actcaacctt
     6841 tataccgttc catgaaacta tcaatatcag attttttaaa tctaatgacc gtatcaattt
     6901 ttataacagg taacccgtat tgagaaacat attttctaat cgttcctact gatacaccta
     6961 gatattcact tgcctgctga tagtccatat actctagagt gactccagaa ataatgtctt
     7021 ctagcatagg ctttagcact gtaattatct ctttcacttg ttgttcaact tgtatagaaa
     7081 caatgctatc aatgatttgt agagacatat ttccccctcc tttcgtgatt gcttgaacaa
     7141 attttctaag aattatcttc gtattttttg ctcaaaataa aagtattttg ttattaaccg
     7201 attaatcaaa caaaaaatga ttaccagttc atcgacatac acataatacc tcgataaaaa
     7261 aggggtgtca atagttttgt aaaatttatt tacaacaccc cttttttgag gtatactaat
     7321 tttaggaggt gtggtcaata tgataaaacg tccaccaata aattatttag aacgaaaaaa
     7381 gatactcgga acaaaaataa aagctataag aaagagtaaa aaattaacac aaccagcatt
     7441 tggtcttatg attaacaacg gtcaattaat tgacaaaaag actatctacg aatgggagaa
     7501 gggaacctat ttaccaatac cagagcgatt atcccgaatt gctgatttgg gaaatatgag
     7561 tatagaggag ttagtttgtg gcaatgtaga agaatatatt ttgggaatta ttctttatag
     7621 agatagcatt gtactagatg gtataacatt tccagataaa aacctgttcc agcatcttcg
     7681 tcaacagttc ccaccagtac actctaattt agatacatgg ctggatagat attccaaatt
     7741 agaaccagag atgcaagaat ttattgctaa caagacttgt aataaagtta aaaatgaaaa
     7801 aattagtctt ttcaatatct taaaaataga agaattattc atcaatgcta ttgtagaaga
     7861 atttgacaat aacattcttt tcctcactag cagtatagaa gaactactag aaagaatggt
     7921 agatgaatgg ctacctattc aattgaaaga catgtcttat cccgaagaag ctgtgagaga
     7981 aattactgat aatataaaca agctagaaca aacgatttca tcaattggga aaaaatacac
     8041 taagaaaaaa atgaagggag gtgataccat ctagtggtaa gtactccttg taaactaaga
     8101 ggagatgatt tcatggcaaa aatatcaaag tacaaaaaga aaaacggtca agtggcgtgg
     8161 atgtataaag gacacattgc caccgatcca cgaactggtg aaaaaataaa tactacgaga
     8221 agaggtttta gtagcaaaca agaagctcaa aaagattatg acgagtacag acatcaaata
     8281 ttatacggtg ttaaaaagaa agcacctgat atgacatttg aagacctgta taatgaatgg
     8341 ataactcatc agcgtaccag tgttaaagct tcaacaatcg caatctcagt aagatacgca
     8401 aataatcaaa tacttccagc atttggtaag cttaaactat cgaatatctc tgtcccatat
     8461 tgtcaaaaag tggtagatga atggcacagt aaatatgaaa gttacgatta tatgagaaaa
     8521 cagactgcac aaattttacg ctacggggtt gcaatgcaat atattgacaa taatcctatg
     8581 gaaaaaacac ttcttccacg aaaaaaagag tatgagaaaa atcgtaaatt ttactcaaaa
     8641 gaggagctta ataatttact ggatgctttt aaagattttg gtaacatgaa acaatacgcc
     8701 ttcttcagat tattggcata cacaggtatg agaaagagtg aagtactcgc tttacagtgg
     8761 aaagacatag acacgtttaa taaagaacta catgtcaata aaactcttgc tgtagatgaa
     8821 tttggtaaag ttataattca atctccgaaa acaagagctt ctagacgagt tatctcttta
     8881 gatactgaga ctctttctat cctcaacaac tggaaattac agcaaaaaga agagtattta
     8941 aaacttggat acaatactag ctctaaagag caacatgttt ttacaacagt taaaaacact
     9001 ctatacattc caaacactgt taatgattgg ttaagatata ttttgaaaaa atataacttg
     9061 cctcgtatta cacctcatgg gtttagacat acacatgcta gtttattatt agaagcagga
     9121 gaatctgtca aggtagttca acaacgatta gggcatgaaa atagtaaagt gacattagac
     9181 atctatgcac atattacaaa taatgcacct aagaaaacag gtcaagactt tgctgatatg
     9241 atggctcatc agtaaaagag ctggtcaaaa tctggtcatt tctagtatac aggcaaaaca
     9301 aaaaagctag aacccttata tatcaagggt tctagctcct ataacaggtt tacccaacac
     9361 tgccttccaa tgactctttt gggaatcaga ggaaagaatc aggctttata gagatatttc
     9421 atctaacaaa ttactttaga ctgacagtat atatcagcag gtactgtcat cgggaacgta
     9481 gccaatatcg tatccaaaac agcaggtaga gaagctattt gaggattcaa gttattctat
     9541 ttcaaccatg aagttaagtt cattaaaaat aaagatagtt ggtatttcaa taatttttgt
     9601 atagagttaa tcactttatt gatgctaatt catcttgatt ggtattataa tcgtattcaa
     9661 gttgcgtggc gtgcttttgc tagaaaatat ataactgttc tttataaaga aatataagga
     9721 ttaaacattt tttatcatag agttttactt atctatagaa ttatgtctgt tcctaattca
     9781 gtttgatgaa ttaactgata aaaaaatatc tactatttaa agatacaggg ataagtaagt
     9841 aaaaaaataa catgtcatta gatagtagac agttccaact ctttatttac cttttttgta
     9901 tgctgcactg tcaatccaag ttcctaaatt gttgtatcca tcatcactta tataatcata
     9961 ttggccatca ataatttcgt caaaccacaa atattctcca tctggttttt gcccaattat
    10021 tttaattact gattgactag tagttgtcat ctggttaaaa tcttttattt tacctatatt
    10081 aacggccaaa atagcatttc ctcgcttata actctcctgg atttcatatt taacatattc
    10141 tcgatcaaga gtttctttac ctatcagcac cacagtcact gatgttccaa ttaattgatt
    10201 atctatccag ttttttatag caatatctcc attacgttta actttctcaa attcagcctt
    10261 atctacaaaa ccagcttcct ttgttcctcg caaagtccag ctgtttctaa ctaccattgc
    10321 tcttgttaca tctgcataat ggaaactaaa aaaaaccctt ctcaccatat taatcctcct
    10381 gaatactatt tattaaactg acaatttgtt caactttttt atctacttct ccttcagtat
    10441 ctaatttttc tagcgcttga aatttacttt ttaaggaatc atcaaaataa tcactgaaat
    10501 tctcttgaat cttatcaaac aaaaccttac ttgcaccttt ggtaaatcca atcggaacta
    10561 ctatattttc attttgaaca gataattcaa attcttcaat cattccatct gctgtaacta
    10621 tttcacctga ttcaagtttg tttccaaaaa caaaaatact aatcccagac tctgcaataa
    10681 tgttttctcg atatcttctc cactcatgag gcatatcaag gggaagtggt cttaaagaaa
    10741 gtacgtcttg tatgttattt tttcttaaat ttcttctctg ctttagaact cctgaaacaa
    10801 tactattacc aattccaagt ccgtaacctg atactattct ataaccggat tcagaaagcc
    10861 tatatgctaa tttctcgaca aattttaatc ctttcgattc agatgtaaat ggagtataat
    10921 caacagcact tcccgaaata aaaatagttt ttctcaaata cttttttcta atacttttca
    10981 aaatatcagt aatctcagaa taatcatcta cttcaacaac gtttatcgaa aatctggaca
    11041 aatcattgat ttgtagtctt ctttttgtca gttcataatt atatctctct tgatcaaaaa
    11101 aatcctcaga atcgacaaaa ttttccttta aaattatatg ggattttaga ttatcttttt
    11161 agaagttctt gaatgtcggt atagcccgtg tttccgggct tttccggcat ttttgatatt
    11221 gttagatact tacaaatgtt cttattacct ctcaccattt ccccctcaaa agtagtaaat
    11281 ggagtagtaa aaccggcctt gtgtcaggct gccagccgtt ccatttctgc ccttgcggaa
    11341 tcgaatgtgg cgtgtgcata atagttcagc gtcatggtga tattggaatg tcccataatg
    11401 tactgcaagg ctttggggtt cattcccgca ttggctaacc gggtgcagaa tgtatgacgc
    11461 atagcatggg gcgtcgtcgt tttcggcaac gcttcttcat ggctcttgtt gtacttcttc
    11521 acaagcctgc caaacatccc gccataatct accgccgtca tgggatagcc gttctgtttg
    11581 agaaacagga aattggcata cccgtcaata atgaacggct ttgcaccttt gcggtttttt
    11641 atcactcgct ggaacgcctg atacacttcc tcattcatgg ggatttttct gacgccgctt
    11701 tgggtcttgg gttcgtcaat atagtaaccg acgccggtat ttctcaaaag ctggtggctc
    11761 acgatgatta cccggttttc aaaatccaaa tctttgtccg tcagtccgca cagttccgaa
    11821 atgcgaagcc ctgtccccag cagaataacg attgcgtcat aatgcttttg gtaaacctta
    11881 tcaccttgaa taaaggacag aaaactttct tcctgtgctg gtgtaagagg cacctttggt
    11941 tccgtatcgt cgtccagaac gtcactaagc tggaagtcaa acgggttttt tcggatgcaa
    12001 tcgtcctgta tggcggtgta aaaagccgct ttcagggaac gcttgtcgtt attgatggtc
    12061 ttataagaaa ggcccttttc tttcatccga agcgcccatt cctttgcgtc ggacagcttc
    12121 acactgtcaa tcgggcagct ccccagccga tcttgttcca gaatacgcat cagccgttcc
    12181 cggcccagtt tggtgttgtg cttcacattg ccccggtgcc tgatctgctt ggcgtagagc
    12241 tggcagacgg tcattttctt gccgatggtg tctatcccgt cgtcaaggtc tttctggatt
    12301 tctttctcct tttcccggag tgaaatatcc tcccgtttac ctgccggggt cttatccgta
    12361 ggcactaact tccatgagta aacaaactgc ggttttccaa aggtatcaac atatttgtaa
    12421 gcatatcgtc cgtcttttct ctggctctct ccagaccgca aaacgcgatt tttgctgtct
    12481 cgtctttttt ctgacatctc gtcatgctcc tttccatgac ggaaagagcc gcatatatgc
    12541 ttataaatta ttataacata tctgcggccc tttttcacta ttagattgtg tccaaagtgt
    12601 caattatttt ctcaaactgc ttgcgtttga tctggatgcg gttgccattc ataatgaccc
    12661 agccagaggc cggattttcc tcggccagct tacgcaattt gttttctccg atacggaaat
    12721 atttagacgc ttcctcaatc gtcagcgtgt atttttccca aataggcaca tcatggttca
    12781 taatgcagcc ccctttcaca aattcaaagg tttgtgtgat ttaggggcat agacggacgg
    12841 ctgctggcac agctccacgg gactttcacc cccgcgtggt tctcacgcgg ccctacccat
    12901 tgcctgcgac gcttttaacg ctcggactgt ggctataagg gagtatcata gtcctgcctg
    12961 tacgtcgtcg cccgcaagcc gccacgctca cattgcggcg tggtgttcct cgctccgctt
    13021 ttcccgtagg gaaaagtatc atggcgcacc agccgtccgg ctcggcacag acagaatgtg
    13081 tccgtatgcc ctatgatgcg ccgccgttat cttgcgggca tatttatcaa ggtgcgatca
    13141 ggccaccggg aaaacaccga aacggggaaa gggtaagccg tttcatgctg ccggggccgg
    13201 ttcgtcagtt cgtgacgata gcgtggatga gcttgatttc cagacggcgc ttcatggcct
    13261 cgtccagaca cacatgcgga tagccgtttt cgtcatagag cgtccgggta cacagcttgt
    13321 tcatgtaacc gctgtaatac tgcaaaacgc gctctacggc tccggcatcc ccagcgcggg
    13381 cggcctcgat caccgaaagg ggcaaaagct caatgccctt gtagttcggg ttcttcatcc
    13441 gcgtctaacc tcccttcggc atcaacgcct ttagctgttt ccgcagctcc gataaggtat
    13501 ttgtcctgtg ccgctgaacg gcactccggg acattcccac aaggccgccg atctcaccat
    13561 ctgccatatc cagaacacag tgcaggatta aaatactctg ctccatgctg ggcagcgcgg
    13621 caaaggcttc ggcaacaagc tcattgttga tatgtaggtc atagccatgc gacgaaaacg
    13681 taaagctgtc gctggggtag tgatctactg tacagagctt gtccaaatct gcctgtgaca
    13741 aagagcttag tgacacttca cggtcacggc ggcgcttcat ttcagccaag tagcttttgg
    13801 cctcgttacg cagtaccgtc ttgcaaaaag catcgaacat gcaccgcgct tcatagtcgc
    13861 gggggatagc atccatcttc tcaccccctt tctgtggggg acttaggtgg ttctctccct
    13921 ttccgctaac aagactttga caaggccgcc agctacccgc taaatacctc ctttttgaaa
    13981 atttctcaaa aaattttttt ggggacagcc gggccgcaaa agacgacaaa aatcgcccgg
    14041 caggtcgcaa acctaccggg cgagaaaatg ctttgctatg ctgctgattt acatggtata
    14101 gtacatactc ataaccggaa tagccctgtg
//
LOCUS       DADVXU010000072        13930 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.075, whole genome shotgun sequence.
ACCESSION   DADVXU010000072 DADVXU010000000
VERSION     DADVXU010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.075"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..132)
                     /locus_tag="KB953_002007"
     CDS             complement(<1..132)
                     /locus_tag="KB953_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBB6866977.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
     gene            541..2913
                     /locus_tag="KB953_002008"
     CDS             541..2913
                     /locus_tag="KB953_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866978.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEEYVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESAFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     RPTDSMKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2974..4434
                     /locus_tag="KB953_002009"
     CDS             2974..4434
                     /locus_tag="KB953_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HBB6866979.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQIKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            4445..6649
                     /gene="topB"
                     /locus_tag="KB953_002010"
     CDS             4445..6649
                     /gene="topB"
                     /locus_tag="KB953_002010"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBB6866980.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6665..6814
                     /locus_tag="KB953_002011"
     CDS             6665..6814
                     /locus_tag="KB953_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866981.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6889..7482
                     /locus_tag="KB953_002012"
     CDS             6889..7482
                     /locus_tag="KB953_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HBB6866982.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            7495..8394
                     /locus_tag="KB953_002013"
     CDS             7495..8394
                     /locus_tag="KB953_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HBB6866983.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            8397..8894
                     /gene="ssb"
                     /locus_tag="KB953_002014"
     CDS             8397..8894
                     /gene="ssb"
                     /locus_tag="KB953_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBB6866984.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            9260..9520
                     /locus_tag="KB953_002015"
     CDS             9260..9520
                     /locus_tag="KB953_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866985.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9965..10171
                     /locus_tag="KB953_002016"
     CDS             9965..10171
                     /locus_tag="KB953_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HBB6866986.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            10219..10422
                     /locus_tag="KB953_002017"
     CDS             10219..10422
                     /locus_tag="KB953_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866987.1"
                     /translation="MVDKEVISEKVAEALLIPRYWTIETPLVEDAPTMLFEDLVKIAG
                     YELHYQENMETYENEYWLTKLEN"
     gene            10438..10875
                     /locus_tag="KB953_002018"
     CDS             10438..10875
                     /locus_tag="KB953_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866988.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            10868..11575
                     /locus_tag="KB953_002019"
     CDS             10868..11575
                     /locus_tag="KB953_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866989.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
     gene            11955..12134
                     /locus_tag="KB953_002020"
     CDS             11955..12134
                     /locus_tag="KB953_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6866990.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            12459..12722
                     /locus_tag="KB953_002021"
     CDS             12459..12722
                     /locus_tag="KB953_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HBB6866991.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            12716..13066
                     /locus_tag="KB953_002022"
     CDS             12716..13066
                     /locus_tag="KB953_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBB6866992.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(13090..13704)
                     /locus_tag="KB953_002023"
     CDS             complement(13090..13704)
                     /locus_tag="KB953_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBB6866993.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
BASE COUNT         5153 a         2301 c         2844 g         3632 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaaatgata ttttacgagg aggaaagaac
      541 atggcagaag aaaaagaacc actagacaat agtcgtttgg gaaaatccaa aagaaaacta
      601 gtacgattgc aaaatgaatt aaacgaacaa attgaaaaga tgtttgaaca ccaaaaaatg
      661 acgaacggtc aaccaatgaa cgacaagagg aatggccgtt catggtttaa ccagcaagaa
      721 cgaatcgaaa ataaagtcca aagtctgaga gaagagatca agcaacaaga agaatacgtg
      781 gaaaagcttg aaagacaaga agagctaaag gaaatgggct ataacaagta cggcggactg
      841 gatatgacga tcgataatat tccaagaatc aaagaagaaa tcgaacgctt tgaaaaaggt
      901 gaatcggctt tttcagccgc cacgatccga aaataccaac ggaagttgga aacattagaa
      961 cagctgaaag aaaaatcaga aaaaggaaaa gaaaatatcc tccctgaagt acaagcgatc
     1021 attgacagcg gacgagtgac gcaatggaaa aaaaatccaa ccatttattt tttgaaaggc
     1081 tatcgaaaag ttgctttaga actgaatgaa aacgggggat ttgaggaatc aaaaaaatac
     1141 caagccacgt cggaagagga aaaagcagct gttcgtgaat tacttgaaga ggctagacca
     1201 actgattcta tgaaggagaa agaaaacgat catttctatc gagtagaatt caacgaaaat
     1261 gatccagaaa aaggattgaa aagttatggt ggggagatcg taacattcga attgatcgat
     1321 caactcaaaa cattggataa ccaatacgcc gcagaagcag gttattataa attctatttt
     1381 gaggaagtga agaacggaga agtagtcgat cagatccgaa tggatctagg ggatggcgtc
     1441 aacagtaatc aaccaatcta tttagagtta gcaaaattgt gcgaacaagt acctgagaag
     1501 gaacaaaaac aatcggtcaa aaatgaacct gtttcgaaag aacaaatgca gtcgaaagaa
     1561 acaaaaacaa aacaaaaggg ctatcaaaaa cgaacggcag aagagattaa acaagaagtt
     1621 aaatcccttt cgcaacaggc actggatgcg gtgaaaaagc atacccattc tcctgaagac
     1681 gtcaaagaac ttttgaactt tatgtctcgt tttccagaac gatcctttag aaatcaatta
     1741 ttgattgagg aacaatttcc aggtgcaacg gcttgtttag gtcgtgccgc gttgaaaaaa
     1801 gaaggaatct atatcaaaaa aggggaacaa gggaacaaga tctttgtcag aaaaacggtc
     1861 aaagggtttt atgagaaagg acgtggcttt gttcgagaaa cagaagctac accagaagat
     1921 aaaaaacgaa tcgaaagtgg tcaaatcgaa gtgatcaaaa aaccttacta tacgattgaa
     1981 aaagtgtttg atatcacaca aacacagtta aaacctgaag actatccgaa aatctttccg
     2041 aaccgtgtct ttgattttca attggataaa aaaggacagt cagaacttca agctgggatt
     2101 caagcggtag ccaacaatat tggaatacaa attcgagata tgacagaaag tgaagtctat
     2161 caaagagaat taggacaagc acgtggggcg tatgttcgta acgaatttac tggcgaagaa
     2221 gaaatcgtca tgaatactcg aaatactcct acgcagcatt tagctacaag tattcatgaa
     2281 ttagcccatg caagaatgca caaattttca gaattagata ccgcaactaa agaattgcag
     2341 gcagaaatga cttcttatat tgtgtgcaaa cattttggaa tggacacgtc ggagaaagct
     2401 attccttata ttgcagcatg gacgaagaat ggtcagacgt tggatgataa ggaagctgcg
     2461 gaacgtggga aaatcatgaa tgatgtcagc cgtgtcgcaa atgaattcat tcaaaccatt
     2521 tctaatgaaa tcaatcaata cagagaaaga gaaccagaga tccaaccagc agaagtgaag
     2581 caggagcata aaaatacttt agaaacgcca gagaataaga atattgtgcc cgttggttca
     2641 ctttggatcg acaagaaaga cgggaaagta atggaagaag atacgggtcg aacgttacat
     2701 ttgaaggatg cggcgtttat tgaagatccg cagcataatc aagtaatttt aaaaggaaaa
     2761 agttatggaa aagagatcca aacggcgtta ccttcagaag aatttgcgct aggaaatcca
     2821 ttccgatttt cacttgagac agtgtcagaa gtggcaaaac gaaaaaatga aatctattat
     2881 gaacaacaat tgtcacaaaa agaacgtgta taaaacaagt aaaaaagact atctgaaaaa
     2941 cggatagtct tttttctttt gggaggtttt aaaatgaccg agaaaaaaat gagtcttatt
     3001 gatcgatgta aacagatcga tatagtggat tttgctcgaa acaatggaat ggctgtagtc
     3061 aataaaggac gagattatcg cttagaagac catgattcat ttgtttttga tcgaagaaaa
     3121 caacgttttt attggaacag tcaaaatatt agtggcgaca ttattgaatt agctaaactt
     3181 tttttcatag acaaagagat ccaagaccct aagcaacaat ttaaagcagc cactgatttt
     3241 attctaaaga atgaagacaa aacagaacga gtggaaaatc ttcattttga aacagaaaag
     3301 tacaaagatc accccgttga ttatcaacca ctcacagaaa aaggaaggaa ttatctgaag
     3361 gaagaacgaa aactaccaga gtggctgatt gattatgcag aaaaagaagg gttgattgcc
     3421 gaactaaagc caaaatatga acggcaaaat ttcttagttc gagacgatcg tttagatcat
     3481 gcggtcgcct ttttatggaa agatccacag acaaaagaga cagtaggggc gagttatcaa
     3541 ggaacgttca ttgattatga acgttttggt gagagaggca catataagca tatagataaa
     3601 aattcgacag cgaatcatgg ctttaaccta aagatcggtg accctaagca gataaaattt
     3661 tttgagagta gtattgattt acttagctat gctgcactga atcgtgatca gttaaacgat
     3721 acgtggttag tctctatgga agggttaaaa caccatgtga tcagtcatta ttttggtgag
     3781 gcagtatctg aattgagaaa gaaacaagcc tttcctcaat ccatcgagat ttgtgtggat
     3841 aatgaccgtg caggacatat tttctacgaa aaagaacaat taatgggcgc agtcgatcca
     3901 tttacgaatc agaaagtacg ttgtgaacga ggaatcgcta atgattggca agtgccaaaa
     3961 gaatacaaaa tcatttatga agaagtcgca aaagaagaaa aggtcacacc agaagctatt
     4021 atggccatac acaagacaga aaacaatctg caattaacga accaactggt ttccgctcac
     4081 aaagtaaatg cgttttttgg ccagcagctt tctgttaacg attcaataga agccattaat
     4141 ttgaaagata tttgtagaga agtcgcaaaa gaattaaaag tttgtgaacg cgtggatggt
     4201 acgtacgatt ttgatcgttt ttatcaagaa aaaggggata ttaatgcgca gattcttttc
     4261 tcttataaag cagaacaata ctacaaaggc tataaaaatc atgaacatga atttgtacct
     4321 gaagtaaaaa aggactggaa cgatcaattg aagcatgaga tccagcaaca agaaatcaga
     4381 aagcaaaaac gtgccatgtt gtttcaacaa ggcagacaac aagaaaggga gtgaaatcgt
     4441 catgatgaaa accgttattt tagcagaaaa gccgtcacaa gcgaaagctt atgccgatag
     4501 cttctctaaa gccactcgaa aagacgggta ttttgaaatc caagatcgat tgtttcctgg
     4561 tgaaactgtg atcacgtatg gttttggtca tttagtcgaa ttagattcgc ccgatatgta
     4621 cgatgaaaat tggaaacaat ggtcattaga acatttgcct attttcccta cacattatca
     4681 ctatcatgtt cctaaagata agaaaaagca attcaaggtg gtgaaacagc aacttcaatc
     4741 ggccgacacc attattattg cgacagatag cgatcgtgaa ggggaactga tcgcttggac
     4801 gatcattcaa caagtaggcg ccgatcaagg aaaaaccttt aaaagacttt ggatcaactc
     4861 cttagaaaaa gaagccattt atcagggatt ccagcagtta cgagacgcaa aagagaccta
     4921 tccaaaattt gaagaagcac aagcacgtca gatcgccgat tggttaattg ggatgaacgg
     4981 tagcccactg tatagtttac tcttgcagca aaaaggcata ccaggaagct tttccttagg
     5041 cagagtccaa accccaacgc tttacatgat ttatcaattg caggaaaaga ttagaaattt
     5101 caagaaagaa ccttattttg aaggaaaatc cgaagtaatc gctcaaaatg gcgcatttga
     5161 tgcgaagctt gatccgaatg aaacccaagc gacgcaagaa gcatttgaag attatttaaa
     5221 agaaaagggg gttcaattgg gaaaacagcc aggcatgatt cgccaagttg aaaccgagaa
     5281 aaaaagcgcg gcaagtccac gtcttttttc tctatccagc cttcagtcta aaatgaatca
     5341 gctcatgaaa gctagtgcca aagacacttt ggaagccatg caaggcttgt atgaagggaa
     5401 atatctaagc tatccgcgaa cggatacccc ttacattacg gaaggggaat atgcgtatct
     5461 gttggatcac ttagacgaat acaagcattt tttgagggct gaagaaattt cgactcctat
     5521 tcatacaccg aatagtaggt acgtgaacaa caagaaagtc caggaacatt acgcaatcat
     5581 tcctacaaaa acagttatga cagaagcgac gtatgaaagt ctgacacctt tacaacaagc
     5641 gatttacgag caagttttaa aaacgacagt ggcgatgttt gcggaaaaat atacctatga
     5701 agaaacaacg atcttgacgc aagtccagca gcttcagtta aaggcaatcg gaaaagtacc
     5761 gatagatcta ggttggaaga agttatttgg caaagagagc gacggaaaag agaaggaaga
     5821 agagccgcta cttcctaaag tgacaaaagg agaaatggtg acggtagatc tacaagtcct
     5881 tgaaaaagaa accaagccac cacaacctta taccgaaggt acgttgatca ctgcgatgaa
     5941 aacagctggg aaaaccgtgg atagtgaaga agcgcaatcc atattgaaag aagtggaagg
     6001 aatcggtaca gaagcgacac gggcgaatat tattgaaacc ttgaaacaaa aggaatatat
     6061 caaggtagag aaaaataagc ttgtggtgac gaataaaggg gttttgcttt gccaagctgt
     6121 ggaaaaagag ccgctgctta cgagtgccga aatgaccgcc aaatgggaaa gctacttgtt
     6181 aaagattgga gaacgaaaag gaacacaaac cacttttcta gccaatatcc aaaagtttgt
     6241 ctctcactta ttggaagtcg tcccaggaca aatccaatcc attgattttg gatcgacgtt
     6301 acaagaagtg aaagcagcca gtgaaaagca agaagccgcg cgtcatttag gaatttgtcc
     6361 aaaatgccag gaacaagaag tcttactcta tcaaaaggtg gcagcttgta cgtctgaagc
     6421 ctgtgatttt aaattatgga caacgattgc caaaaagaaa ctcacagcga cacagctgaa
     6481 ggagattatt caaaatagcc gaacaagcca accagtgaaa gggttaaaag gtcagaaggg
     6541 ttcgtttgaa gcaacgattg ttttaaaaga agacttcact acaggctttg aattttctga
     6601 aaagaaaaaa acgaacttca agaaaagaac acgacgcacg acaaaataat ggagggttaa
     6661 tcttatgcgt aatacaacac acaaacaaca agaaatcgaa aatcaaaaag ccaaaattcg
     6721 ttcagaacaa gagaaattac gtgccagtaa aagaagactt cagcaattaa agaaccagca
     6781 acggattcaa aaagaaatcg aattagatcg atagacatta accttgttac aaatacacta
     6841 tcatgatata tttaatgtag tgtatttgta acaaaagggg ggaagtaaat gcaagagaaa
     6901 ctgaaagaat tgttggctat tcgcgatgga aatttatcta tgaaggaagc cagaaaatta
     6961 ggaatcgcag caaccactgt acaaagattg gtgaatcaag gaaaattgat caaagttgat
     7021 cgtggttatt atgtagagga tggtcatgga atagatgatt tattttggct tcagcaacgt
     7081 ttttccaggg gaatcttctc tcatgaaacg actttagata tttaccagct ttctacgaat
     7141 atgccgaaat tcattcactt aacttttccc catggatata acgttgatcg ttcaattaca
     7201 aaagagcagc agttacaatt tcatttcgtg aagaaagaag tttatgaact aggaaaagta
     7261 gagggaactt cctttcaagg aaatcctatt actatgtatg ataaagagcg tacactttgt
     7321 gatatttgga atccgagata tgaaattgaa tatgaaatga aattagaagc attaaaagag
     7381 tacatggaag acccattgag agatccaagt aaattaagag attacatgac aaaactacat
     7441 gtagaaaaag aaatgaaata ccacatgcaa agcttatatt gatggaggaa aaagatgaca
     7501 ccgacacaat ttgcagcaaa aacaagaaat ttagcaaaga ggacaggatt aaatgcacag
     7561 ttagtacaac ggcattttat gatggatcgt ttaattgaac aaatttctga atctaagtat
     7621 aaaaatgatt tcattttgaa aggtggcttt ttattaggat caaaatatgg aatcgataga
     7681 aggacgacaa ttgatatcga cacgacattt agaaatagca aattaactga agaaaaattg
     7741 aagagtattt ttaatgattt aactaggaaa cccacgaaag aaggtattgt tttctctatt
     7801 caaggaatga aggaaacaag agaagccgat ttttatcctg gatttcaaat taaattggtg
     7861 gctaatttag agaaagtacg tgtgccattt aaaatggacg tgacaacagg agattctatt
     7921 tatcctgaag tagatacaca ctatcaccaa ttaatgtttg aagataaaaa ggtggagatt
     7981 ccagcttatc caacagcaca aattattgca gaaaagctaa gtgctacgtt cagctttggc
     8041 acagataatt cgagagcaaa agattactac gacttattta ccattccgaa actagaaaat
     8101 atcgatacga aagagttata caaaagtgtt cgaaatacat ttacaaaacg tggggataca
     8161 aaaccgttgt ccttttatta tgaaagagaa atggataaga tcataggaaa tgactatttg
     8221 gaaaagcagt gggaaaaata tcgctcagtt aacacgtttg cgaattccat ttcatttaca
     8281 gatacagtca acgaaattga tcacctaatg aaaagtgtga tcaaaattga aaatcaagaa
     8341 agaaaacggc agtttaaaag atcacaacaa atgaatcaag aaatggagag ataaagatga
     8401 tcaataatgt aactttagta ggacgcttaa ccaaagatcc agatttacgt tataccgcga
     8461 gtggtactgc agtagcgact tttaccctag ctgtcaaccg aaatttcacg aatcaaaacg
     8521 gcaatcgaga agctgatttt attaactgtg tgatttggcg caaaccagca gaaacaatgg
     8581 tgacattagc taaaaaaggt agcttgattg gtgtagttgg gcggattcaa acgcggactt
     8641 atgacaacca gcaaggacaa cgtgtgtatg tgacagaagt tgtggccgat aatttccaac
     8701 tattagaaag caaagcagcg acagaaagcc gtgcgcacgc tgatcaatcg tccaccagtc
     8761 caagcaccac cacttttgaa caacgggata cagcgacgcc taataacaat ggattgaacg
     8821 ctttacagaa tccatttggg aatccatttg gtggacaatc aatcgatatt agcgatgatg
     8881 atctaccgtt ctaagtagca ttttctattg gttgcatttg ctacggttta tgttataatt
     8941 tagtcaacaa aaagaatttt caatctataa ccaaaaacca ctagctggcg ggctagtggt
     9001 tttcttttac gtttctatta taagaattaa cgggtaggct tactgtcttt cttggcaaat
     9061 ttacaatcaa gccatttgca gatatagtgc gcggctactt ctgccatgat tgcaaaaaca
     9121 aaatcaaaaa gaattttcaa acctataacc tcctcccctt gccagattag gccggggtag
     9181 cagacagcaa gactattata gcataattga aatatccaaa tgattggatt tttgctataa
     9241 ttttagcgag gtgattgtga tgaacaaata tgttggtttg cttgataaaa tccgagtaat
     9301 aaaaacgcaa ccgttattgg ttcgctttac tttacaaacg attcatgaat cgattaattg
     9361 tgtagttgca gatattgaaa ttatcgataa attgctaatt atggatgatg gaaaatacaa
     9421 tattgccgtc acaggtcact tcaataaacg aaatcagcta gttattgcta gcatgtatgt
     9481 tagaaatccc gatcatttta cgcgatcgat gggcatataa aaaaagaaga gttgcaatcc
     9541 taatgtagga ctgcagctct ttttgtttta catagaaaca agaaaaatga aaaggccttt
     9601 ttaggaaggc gcgagcgcct tctcaattga aaaacctctc tcactccaac cgccaccgcc
     9661 gttcgagaag aaaggaagtg aacgttgcac gttcactaag ataaagggtc ttgcaaagca
     9721 agacaaaata gaaaaaactt cctctcacca aaaggttcgt tcctgttcgt tatctcttta
     9781 ttgatttgcc agaccggcaa caagttgcct tttatcacca aaaagcgaca aggacaaggg
     9841 taaatggcac gctgcgcgcc cttgtccttg tcgcttttcg ttgattcatt ctggccaaca
     9901 aatcaataaa gaaagaacta acgaaaggaa gtaaaacctt tcctaagagg aggcagaatc
     9961 aatcatgcga aagaaatttg attattgggg tgttcccttc agcgaattat tcccaaacta
    10021 tcatgcacca catactgtcg aatgcgattg tggacaaaga gcgaaatgta tcaaaagcta
    10081 tcgtttgtat caatgtccaa catgcggaaa aaaatacacc ttgtcttatg gcgattatat
    10141 tttgattgat gagaaaggaa agaaacgctg atgaaaacat aacataaatt attgactgta
    10201 aaagaagtcg ttcaaatttt ggtggacaaa gaagtcattt ctgaaaaagt agcagaagct
    10261 ttacttattc cgcggtactg gacgatagaa acccctttag tagaggatgc accgactatg
    10321 ctatttgaag acctagtgaa gattgcagga tatgaactgc attatcaaga aaacatggaa
    10381 acttatgaaa acgaatattg gttaaccaaa ttagagaact aaaaaggaga aaaaaatatg
    10441 gctcaaaatt actatgacga atttgtcaaa ttacctttgg ataaaatggc acaaaaaatg
    10501 gaggatatga cattcctata ccatgagaca cgtgttccaa aaaaacatta taaggaaaga
    10561 ttgtcggtag ctgtggaaga aatgattgaa tcaggtgtcg aaatgaatct cattgcgacg
    10621 tactatcgaa cactggaaga actgaaaaaa caaaatgcaa aatggttttt ccaagcctta
    10681 ctatgcttgg aagtaggtgt caaaccaagc actattaaac cttctgaata tcaagcttta
    10741 gaactaacgt atgggaaatt tgtcgaaacg aaaaaagcca agactgtttc aagtgaatgg
    10801 ttagattatt ttgaaaatat caataagtat ggcgcctatt acaccatgaa aaaggaggac
    10861 aacgaaaatg aataaggtga tttatcaact aacaacggaa gatgtgtcac actttttaac
    10921 aaacaaagaa catttgaatg aagtaatcag acggattcaa aaagacttta gttgtatgga
    10981 agaaatgaag tactttattc aaagagtcga agaacaattg tatttggaaa gtgaaacgag
    11041 tcaaacgaag gtcacagtca gcaataatta tttagacgtg tataaacatc gtcctgaaac
    11101 ggtaaaaaaa ggaacggagc ttctacgact agaaacagaa aaatacatca tgaatgttta
    11161 cctagaaagg agtgaactgg tcgaaaactt tgtcagctgc acgatttgca ggaaacacta
    11221 ttactcagaa tcagaatatt attcaatttt tgagagcatg gaattaatgg aagaagaaat
    11281 ggaagagcta aacagcaaaa atgcgacgat agacgattat tatcttgtat ttactagatt
    11341 tttgacgtgt ggaacggaat ctatcgtgtt aatgggaatg gattatgaac tcgctgtacc
    11401 cgaagcattt ttgagggaat atgtagaaga agtagataac ccaggcgacg actggtacac
    11461 gactttaact gattttttag atacctacac ttacgacgat acagaagaaa tccaaaatga
    11521 acttgaccgt aaacgaatgc catatcaacg atttgaaagg aaaaaaaggc aataatcatt
    11581 tataccgaaa agtgagaatg tgtgaaataa gaacaaaaac cttgaaatta agggaatggt
    11641 tcttatttcg caccaatcgc tccaaccgcc cccgccgctt aggtggtgcg aaataagcgg
    11701 gcaaagcccg ataacctaag agaaagaagg agatccatat gaataaaatc attcctctgt
    11761 tgttcgtact gtgtattggc gagatactta tgcgtcatgg tgcagctgta catttaaact
    11821 tcgtagtatt cttaggaata ctagtatttt gtttgatttt ttggcgcttt gtgttagccc
    11881 ttgtgagtct actattcaag ttgattgcag ccatattttt catagggtta tttcgatgaa
    11941 aggagagagt actaatgcca attgcaatag gaaataaacg gttacccgtg acattagatg
    12001 aaaagagaca aaaagaattg cagcaactaa agcagaagta cggaaaaagt gaatctaaga
    12061 ttatgtgtat tgcgttagat ctattgatcg cgcaagaaaa agcaggattt gatataccag
    12121 cactaaaaaa gtgacgtcac ttttcatttg aagaaacaaa agtgatagca cttaattgtt
    12181 cctactattt agaatatttt ctatgtcctt tttattccat tttgtatcaa taactatgaa
    12241 atctttaaag tatccagatc aatttttttc ttacctgatt ctaaagattt tgttttctta
    12301 aacatattat gatttccttt cgactatttt catataaatg ttaacatagt ttatggattc
    12361 atggaatgtt aatctaattt aatagaaaag gataattaag tatatacgtt ttttcttatg
    12421 tatgttatac tatgtatata cagaaagagg tgtctactat ggaaatgtta cctataaaaa
    12481 aatggggaaa tagtaatggc cttagattgc caaagtatat tatggaatat cttggaattc
    12541 atacagaaga caaagtgaag ataatacaag aagaagtaaa cggtcagaag cggctaatta
    12601 tcgaagcggc taatatagaa aatgaattaa caattgaaca gttatttgaa aattacactg
    12661 atgaaagaaa tcatgtgacg attcaaaatt taggtgaagc agtaggtaat gaaaaatggt
    12721 gagaaaacct agacaaggcg atattctatt attgaataca gcacctcgat ctggacacga
    12781 acagacaggt aaaagacctt atattgtttt aagtcatgat attattgcgg attatagtaa
    12841 cgttgttaca gttgctccaa tttctagtac aactagaaac tatccgctat atgtaaatat
    12901 aaactcagaa tatgaaatga agacaacagg aaaagtatta ttagatcaac taacgacaat
    12961 tgattatgaa gccagagagt gtattttttt ggaaaaagcg catgatagtt tagttgagga
    13021 gttattgatg aaagttagaa cagtttttca aaaagtaagc aaataaaata gaaacagcag
    13081 catgatcaac tattcgtgat tgaccatgct gtcttttttt attctatata cggtttgtct
    13141 cgtaacattg tagtcttttg caatcttagc gatcgcaaca ccttttttta gatcctcaac
    13201 gatgttttta taaacaagac gacgttgggg atcttttgca ttggcactgt atagcttagg
    13261 acgtccttta taaacaccgt tcgcttttgc aatcttaatg ccttgtgcct gacgtcgttt
    13321 agattcggta cgttcttgtt cagcgatcat ggccaaaatt tgaatgatca aatctttaat
    13381 gaatttatcc aggagaggat tgccaattgc ttcggccatg atcggtagac ttgtgatgat
    13441 cagctgcaca tttttcttct tcaagtaatt gactgaagcg atgatctcgt cgtaatttct
    13501 tccaagtcga tcaatagctt ccacaataaa aatatcttga tctcggacga aatccagtgc
    13561 ttcttgaaaa acaggtcgat gagtaatcgt tgcgccagat tgtttctcaa tgaaaattct
    13621 ctctgcaccg aattttttca tttcttcaat ctgccgttgt tcattttgat cgatggatga
    13681 gacacgtatg tatgcaactc tcaaaatttt tacctacttt ctgcaactat ttaaaaaaca
    13741 ccctaatcat acacgagaaa accagatttt tcaatgtcgc ggtcgtgtct agttagggta
    13801 taccttatat agacaaaaac taatgatgga ggaaatagta tgtgtcactt ttctttttaa
    13861 atggcaatat tttttgtaaa taaatgtcaa tcaagaaaat ttaaacgatt atgacattaa
    13921 aaatatattt
//
LOCUS       DADVXU010000073        13657 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.076, whole genome shotgun sequence.
ACCESSION   DADVXU010000073 DADVXU010000000
VERSION     DADVXU010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.076"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            59..301
                     /gene="yaaA"
                     /locus_tag="KB953_002024"
     CDS             59..301
                     /gene="yaaA"
                     /locus_tag="KB953_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HBB6866994.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            288..1412
                     /gene="recF"
                     /locus_tag="KB953_002025"
     CDS             288..1412
                     /gene="recF"
                     /locus_tag="KB953_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBB6866995.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1409..3355
                     /gene="gyrB"
                     /locus_tag="KB953_002026"
     CDS             1409..3355
                     /gene="gyrB"
                     /locus_tag="KB953_002026"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBB6866996.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3375..5846
                     /gene="gyrA"
                     /locus_tag="KB953_002027"
     CDS             3375..5846
                     /gene="gyrA"
                     /locus_tag="KB953_002027"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HBB6866997.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6082..6381
                     /locus_tag="KB953_002028"
     CDS             6082..6381
                     /locus_tag="KB953_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBB6866998.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6431..6943
                     /gene="ssb"
                     /locus_tag="KB953_002029"
     CDS             6431..6943
                     /gene="ssb"
                     /locus_tag="KB953_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBB6866999.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6969..7205
                     /locus_tag="KB953_002030"
     CDS             6969..7205
                     /locus_tag="KB953_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBB6867000.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7348..9321
                     /locus_tag="KB953_002031"
     CDS             7348..9321
                     /locus_tag="KB953_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HBB6867001.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9327..9779
                     /gene="rplI"
                     /locus_tag="KB953_002032"
     CDS             9327..9779
                     /gene="rplI"
                     /locus_tag="KB953_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBB6867002.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10053..11420
                     /gene="dnaB"
                     /locus_tag="KB953_002033"
     CDS             10053..11420
                     /gene="dnaB"
                     /locus_tag="KB953_002033"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBB6867003.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            12200..13492
                     /locus_tag="KB953_002034"
     CDS             12200..13492
                     /locus_tag="KB953_002034"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBB6867004.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         4555 a         2468 c         3009 g         3625 t
ORIGIN      
        1 ttgcgaaaaa aaaggtataa tagagtatac ttttaataat gaaaatgagg gatgggcatt
       61 gaaacaacaa gtcattctaa atacggaatt catgacgttg ggccaaatgt tgaaagaagt
      121 cacagttatc ggtagtggcg gacaagcaaa atggtatttg gcagaaaaca ctgtgttagt
      181 cgatggcgaa ccagaaaatc gtcgtggacg caaactttat cccggcatga tggttgaagt
      241 tccagaagta ggaacttttt ttatggtaaa aaaaggaaac gacgataatg aggctgaata
      301 agctgtattt aaaaaattat cgaaattacg aagagctgaa catcgaattt tccaagaacc
      361 tcgtcatctt tttaggagaa aatgcgcaag gaaaaacgaa tcttttggaa agtatctacg
      421 tattagctat gacgcgaagt catcggacaa gtagtgagca ggaattgatt ggctggactg
      481 acgatcaggc gatgattcaa ggagagatca caaaaggttc atccactctt ccattagaaa
      541 tcttattatc taaaaaaggc cgaaaaacca agatcaacca tatcgaacag aaaaagttaa
      601 gtacttacgt gggacaaatg aatgtcattt tatttgcacc agaagatctt tctttagtaa
      661 aaggcagccc gcagatcagg cggaagtttt tagatatgga attaggccag atcaatccaa
      721 tctatcttta tgatcttgtc caataccaag caattttgaa acaaagaaac cagtacttga
      781 agcaattgaa tgaaaaaaaa caaactgatt tgctttattt ggatattttg tccgaacaac
      841 tagcagcgtt tggcagcaaa gtattgaaag caagagcaca gttcgtcaag cgattggagt
      901 tttgggctaa ttctttgcat cagcaaatca cccatcaaaa agagcagctg gaaatcgagt
      961 atctcaccgc agtcgattct ttagaaacac atacgcaaga gcaaatccaa gaacaatttc
     1021 tcgcactttt gaaccaaaat aaaaagaaag acctttttcg gggaacaacg acagttggtc
     1081 ctcatcgtga cgatctttcc ttttttatta atcagaaaaa tgtgcagact tatggatcgc
     1141 aaggacagca aagaacaact gcactaagtg tcaaattagc agaaatcgat ttgattaaag
     1201 aagagacagg ggaatatcca atccttttgt tagatgatgt gatgagcgag ctggatgata
     1261 atcgtcaact ccatctgcta gaaacaatcg aaggaaaagt tcaaacgttc ttaacaacaa
     1321 cgacacttga ccatgtaaaa aataaaatga ctgttgaacc tgagattttt tatgttcaac
     1381 aagggaaact aaaaggagaa gaaactgaat gacagaagaa agaagtttag tagaacgcgc
     1441 taaagagtat gatgccagtc agattcaagt acttgaagga cttgaagccg ttcgtaaacg
     1501 acctggcatg tatatcggtt cgactagttc tgagggtctg catcaccttg tttgggaaat
     1561 tgtcgataat tcgatcgatg aagtattagc aggttttgca acgaaaatcc atgtgattat
     1621 tgaaaaagac aacagtatca ccgttattga cgacggtcgg gggatacctg tagatatcca
     1681 ggcaaaaact ggacgtcctg cagttgaaac ggtctttact gttttacatg ctggaggaaa
     1741 attcggcggt ggtgggtaca aagtttctgg tggacttcac ggagtaggat cttctgttgt
     1801 aaacgcgctt tctacacagc ttgatgtcaa agtatacaaa gaaggaaatg tgtactatca
     1861 agaatatcgt cgtggagctg tagtggacga cttaaagatt atcgaacaaa cggatcgcca
     1921 tggaacaacg gttcacttta cgccagaccc tgaaattttt acagagacaa ctgagtttga
     1981 tttttcaaaa ttggctaccc ggatccgtga acttgctttc ttgaaccgcg ggatgcgtat
     2041 atcgattgaa gacaaacgag aagaagaacc agtcatcaat gaataccatt atgacggcgg
     2101 gatcaaaagt tatgtcgaat acttgaatgc aaacaaaacc gtgatttttc cagaacctgt
     2161 atatttggaa ggtgaacagc aagatatcgc agttgaagtc tctatgcaat atacagacgg
     2221 ctaccattcc aatatcatga gttttgccaa caatatccat acgtatgaag gtggaacaca
     2281 tgaatcagga ttcaagactg ctttaactcg tgtgataaat gattatgccc gaaaacaaaa
     2341 gctgatgaaa gaaaatgacg acaacttgac cggtgaagac gtgcgagaag gattgacagc
     2401 tgttatttct atcaagcatc ctgaccctca attcgaagga caaacaaaaa caaaattggg
     2461 caattccgaa gtacggacag tcactgatcg gttatttagc gaacatttca tgaaattctt
     2521 gctggaaaac ccaagtgtgg gccggcaaat tgttgaaaaa ggattactag cttccagagc
     2581 tcgtctagca gcgaaacgtg cgcgtgaagt aacgagacgt aaaggtgctt tagagattag
     2641 taatctgcca ggaaaattag cggactgctc tagcaacgat cctgaaaaat gcgaattgtt
     2701 catcgtcgaa ggagattcgg ccggcggatc agctaagcaa ggtcgtaatc gagagtttca
     2761 agccatcttg cctattcgtg ggaaaatctt gaatgttgaa aaagcaagca tggacaagat
     2821 cttggcaaat gaagagatcc ggtctttatt tactgctatg ggtacagggt ttggagaaga
     2881 ttttgatgta tcgaaagctc gctaccacaa attagttatc atgactgatg ccgatgtcga
     2941 cggcgcgcat atccgtacat tgctgctgac tcttttttat cgttacatgc gtcctattgt
     3001 tgaagctgga tacgtctata ttgctcagcc acctttatac ggggtaaaac aagggaagaa
     3061 tattacgtat gtacaaccag gaaaaaatgc agaagaagag ttgaaacaag tcgttgcttc
     3121 acttcctgcc tcaccaaaac caagcgttca gcgttataaa ggacttggag aaatggatga
     3181 tcatcaacta tgggaaacaa ctatggaccc aagcaatcgg atgatggccc gcgtcagtgt
     3241 ggacgatgct attgcagcag atcagatatt tgaaatgttg atgggggatc gagtagaacc
     3301 ccgtcgtgca ttcatcgagg aaaatgcaca ctatgtgaag aacttagata tttaatcatg
     3361 aaggaggaaa aagaatgagt gaagaaatca gagaaaacat ccatgatgtc aatctaacca
     3421 gcgaaatgaa agactccttc atcgattatg cgatgagtgt tattgtcgca cgtgcacttc
     3481 ctgatgtcag agatggtctg aaacccgttc atcgccgtat tttatacggc atgaacgaat
     3541 taggtgtaac accggataaa gcccataaaa aatctgccag aatcgtcgga gatgtcatgg
     3601 gtaaatacca tcctcatgga gacattgcga tctatgagtc aatggtacgg atggcacagc
     3661 cattcagcta ccgttatatg ctagtggatg gtcatggaaa ctttggttct gttgacggtg
     3721 acggtgccgc cgctatgcgt tataccgaag cacggatgag caaaatcgct acagaaatgc
     3781 tgcgtgatat caataagaac actgttgatt tccaaagcaa ctatgatgat acagaaaaag
     3841 aaccagttgt actacctgct cgcttcccga atcttttagt gaacggaaca acggggatcg
     3901 ccgtaggtat ggccacgaat atcccacctc ataacttgtc tgaggtagta gcggctatcg
     3961 atttgctgat ggataatcct gatgtgacga ccaatgaatt gatggaagta ttacctggac
     4021 cagattttcc aacaggtggt ttggttatgg ggaaatcagg aatccgaaga gcgtatgaaa
     4081 caggtaaggg ttctattacc gtacgtgcaa aagttgagat tactgagatg ccaaatggta
     4141 aagaacggat catcgtgaca gaattgcctt atatggtcaa taaagcaaaa ttgatcgaac
     4201 ggatttctga actgcacaga gacaagcgta tcgaagggat cacagatttg cgggacgaat
     4261 cttcccgtga aggtatgcgg atcgtcattg acatccgtcg tgacgccagc gcatccgtta
     4321 tcttgaataa cttatataaa ttgacatcct tgcaaaattc atttggattc aatatgttgg
     4381 cgatcgaaaa aggtgtgccg aaagttttga gcttgaaaca aatccttgaa aactatatcg
     4441 aacatcaacg cgaagtcatc acacgacgga cggtctttga aaaagaaaaa gcagaagcaa
     4501 gagcacacat cttagaaggt ttgcgtattg ctttggatca tatcgatgaa atcattgcga
     4561 ttatccgcgg gtctaaatca gatgacgagg caaaagcaac aatgatcgaa cggttcgatt
     4621 tatccgatcg ccagtctcaa gctattttag atatgcgttt acgccgattg accggtttgg
     4681 aacgggaaaa aatcgaaaat gaataccaag aactcttaaa actgattgaa gatttaactg
     4741 acattctggc aagaccagaa cgtgttacag aaatcatcaa gaatgaatta gcagacttga
     4801 atcaacgatt tggtgataaa cgacgcacag aattactagt cggtgaggtc ttaagtctgg
     4861 aagatgaaga tttaatcgaa gaagaagaaa tcgttattac attgacaaac aatggttaca
     4921 tcaaacgttt ggcaaataat gaattcagag ctcaacgaag gggtggacgt ggtgtccaag
     4981 gaatgggtat ccacaatgac gacttcgtca aaaatctggt ttcttgttct acccatgata
     5041 cattgttatt ctttacgaat aacggaaaag tgtatcgggc aaaaggatac gaaattccag
     5101 aatacgggcg tacagccaaa ggaatcccaa tcatcaattt actaggtatc gattcgagtg
     5161 aaagcatcca ggcaatcatt gcggtgacgg gagaagcaga agaaggccac tatctcttct
     5221 ttacgacgcg tcaaggtaca gtaaaacgta cttctgtcac tgcttttgct aacatccgaa
     5281 gtaatggtct gatcgcaatt ggtttgaaag aagacgatga gctcgtcaat gtgctactga
     5341 cagatggtca atctaatatc attattggaa cacataacgg ctactctgtc actttcaaag
     5401 aagaagcagt ccgcgatatg ggacgtacag cttccggggt cagagggata cgcctgcgtg
     5461 aggaagatta tgtcgttggg gcagccttaa tgaaggaaaa tcaagaagta ttagtgatca
     5521 cagaaaaagg ttacggaaaa cggacaaaag tatcggaata tccagtgaaa ggccgcggag
     5581 gtaaagggat caaaacggcg aacatcactg aaaaaaatgg tccactagct ggattgacca
     5641 ctgtttctgg agatgaagac atcatgctga ttacagataa aggtgtcatt atccgtttca
     5701 acgtatcgac tgtttctcaa actggacgtt caacattagg cgtacgtttg atgaagatgg
     5761 aagctgatac gaaagttgtc acgatggcag cagtcgaacc agaagctgca gaacaaacag
     5821 ttgaagaaca aggatcagaa gaataaccat caagactaag tgtgtaagct agcaagaata
     5881 accgattaaa aaatctggtt attcctgctg gcttttttga ttagttgaat ttctcttaaa
     5941 atattcgaaa ctgttcattc tgtattgctt ttgaccaaaa tagagtgtat aattagagct
     6001 tgtgagtagt ctaattaatt agataactct ccttgctctt aggaacctaa gagccgaaag
     6061 tccacaggga ggtgaatatc aatggaaaac acgaaatatg aaatcatgta cattattcgt
     6121 ccaaacattg atgaagaagc aaaaactgct ttaatcgaac gtttcgatgc aatcttgaaa
     6181 gataacggag cagaagttat cgaatcaaaa gattgggaaa aacgccgttt cgcttatgaa
     6241 atgaacggtt accgtgaagg tatctatcac atcgttaaag tatcttctcc atcatcagca
     6301 aatgctgtca atgaatttga ccgtcttgct aaaatcaacg acgatatcat tcgtcacatg
     6361 attgttaaag aagaagctta attgttgttt catgtgaaac aatagaaaac gatgagagga
     6421 gatgactatt ttgattaata atgttgtatt agttggacgt ctgacgaaag atccagattt
     6481 gcgttacaca gcaagtggta cggcagtcgc aacatttact ttggctgtga atcgtaattt
     6541 tacaaaccaa agcggcaatc gcgaggctga ttttatcaac tgtgtgattt ggcgcaaatc
     6601 agcagaaact ttagcaaact atgctcgtaa aggaacactt ttaggtgtta ctggacgtat
     6661 ccaaactcgt tcttatgata atcagcaagg acaacgtgta tacgtgacag aagtagtagc
     6721 tgacaacttc caattgctgg aaagtcgttc tgcatctgaa aatcgccaac aaagcggcgg
     6781 attccaaagt tctggtcaat cagctggcgg atttggcgga aacaataact ctaatcaaac
     6841 atctcaatca tccaacggta tgcctgattt cgaccgtgat acttctgacc cattcggctc
     6901 aagttcaacg atcgacattt cagacgatga tttaccattc taaacgaatt tgataggagg
     6961 gaaatagaat ggcacaacaa agaagaggcg gacgtaaacg tcgtaaagta gactacattg
     7021 ctgctaacca tattgaatat atcgattata aagatatcga attactaaaa agatttattt
     7081 cagaacgcgg taaaatcttg ccacgtcgtg taactggtac aggcgctaaa aaccaacgta
     7141 aaattactgt tgcaatcaaa cgcgctcgta tcatgggctt gctacctttc gttagcgaag
     7201 actaataaat cctaggaatt gaatgactga tccttttgat cagctattca attccttttt
     7261 ttctgtaaaa ataacctcta ttcgtttagt cgctcgaatg acgaaagtta tggtaaaatc
     7321 aaattagtca tatatgggag gaaaattatg gaaaataaaa acaatcattt atccccgaaa
     7381 cctctcttcc ttatcctgtt tatattaatc gaagtattgg tagtcttaat tttaccattt
     7441 cgttttgtta gcgcttcggt cattctattg atgaatttat ggggtgttcg ctattggttg
     7501 aaaatgaaac gttggcttat tttaaataaa cgtgaaaaaa tccagcaggc ttcgactgta
     7561 gctgaaacca atctagctta tgtctcagaa gtcatgcctg taggagtgat tgcttatcag
     7621 ccatcaagcc aaaaaatcga atggctcaat ccattagcat tgaaaatcaa agatcaaagt
     7681 ggattaacag aggaagaatt gctagatact ttttttactg caaaagaaaa aaagaaaaac
     7741 caagtctatc tagcagataa aacatttcag tttgatatgg atacagtaaa gggtgttgtt
     7801 tattttaatg atattaccca aaaccagttg ttaaaactcc atcagacgaa cagtcagccg
     7861 gtgattggta ttttatctat tgataactat gcagatgcca ttgataaatt agatgacaaa
     7921 gagatttctt atttgaactc tttcgtcact acactagttt ctgactggat gaatgaatac
     7981 catgtctttt tcaaaagaat caacgcagaa cggtttttct tcgtagcaca gatgatcgat
     8041 ctgaaaaaga tgatggataa aaaatttgat ttgctggatc gctttcgagc agaagctgaa
     8101 aaacagagct cgcctatcac ccttagtatg gggattgctt atggctcaag aaccctcgat
     8161 gaaatcggtg gagaagctca gaataattta gatgttgctt tagtccgtgg aggcgaccaa
     8221 gttgtcgtga aagaggtaaa agaaggagca aaacctgttt attatggggg gaaatctgct
     8281 aagaatgtca aacgcacgcg tgttcgttct cgagcgatga gtaccgcttt gcgtcggatt
     8341 tttgatgaaa caggcgatgt gtttatcatg gggcacaagt ttccggatat ggatgcttta
     8401 ggctctgctt ttggtgtcag ttgtctggca cgcttcaacc agaaaaaagc ttacatcgtt
     8461 atcaatgaga acgagatcat tccagatgta gaacggtgtc ttgaagaaat ccataagcat
     8521 cctgaattag aaatgcaact aatctctcca gaacgtgcca tagagctgaa aaaagaagag
     8581 gacctcctcg tgatggtgga ttaccataaa ccttctctat cgatctctca gggcctatat
     8641 gagcaatttg ataagattgt gatcattgat caccatcgtc gtggggaaga gtttccaagt
     8701 aaacctctgc tgacttatat tgaatcttca gcttcttcag cttcagagct agtttctgag
     8761 ctgatccaat accaaagcag caaaacaaat cgcttagatc gttttgaggc cacattattg
     8821 cttgcaggga tggttgtaga tacaaatagt ttcaatgtcc gtacaactgc acgaactttt
     8881 gacgttgcca gctatttaag gtcttgtgga gcagacgcat ccttagtaca atatttattg
     8941 agctcggatt tagcgacata cttggagatc agcaatttga ttgctggaag cgactatgtg
     9001 acttctgata ttgttgtggc ggcagggaca gacgaaaaag agtataatag tgttatagca
     9061 gcaaaaacag cagatacatt gctttcgatg atcaatatca atgcagcttt cgtcattaca
     9121 aagcggacag acggcttgat tgggatcagt gccagaagta ccggcacgat caacgtccaa
     9181 atcatcatgg aatccttagg tggcggagga cacttcacaa acgcagctac tcaactggaa
     9241 aatacgacag ttgaggaaac aaaacagaag ctatttgctg caatcaaaga aaatatgaat
     9301 caaatatatg ataaggagta atccaaatga aagtaatttt tttacaagat gtaaaaggaa
     9361 aaggtaaaaa aggggacatc aaagaagttc cagcaggtta tgcacaaaat tatctaatca
     9421 aaaataactt ggcaaaagaa gcaacgaaaa gcagtatcag tcagttagca ggacaaaaga
     9481 aagcacaaga aaaacacgaa gctgaaatct tggaagaagc taaaaagtta aaagaattct
     9541 tggaaaaaga agatactgta gtcgaattga aggcgaaagc aggggaagac agccgtttgt
     9601 ttggttcgat cccttcaaaa caaattgctg aagggttaaa caaacaatac aaaatcaaat
     9661 tagataaacg aaaaatcgaa ttagaaaatc cgatccgttc actaggctac acaaacgtac
     9721 ctgttaagct tcatcatgag gtgacggcta cgatcaaagt tcatgtcgta caagaataac
     9781 aaatcttgat agcgatgttc agctggtaag tataaccaga aggaaaaaag acaaaccgaa
     9841 atttatctga gaaaacagct gcagcaatga aagcaaaagc tgcaaatcga tttcagattg
     9901 atttcggttt cttttttggg aacagcaaaa tagcataaag aaaacgagta taataaaaga
     9961 gaactatttt tttattacac tttcggtacg aatgatgact tttctctcat tttcttgtaa
    10021 aatgaagcaa ttgaagacaa ggagcgatac gtatgaatga agtgtggcag gatcgaatcc
    10081 caccgcaaaa tattgaagct gaacaagcgg ttttaggctc tgtttttctt gacgcagaaa
    10141 cgatcattga tgccatggag tacatagagc caaaagactt ttatcgtcgt ggccatcaaa
    10201 ttatttttga gacaatgatc gagttaaatg atcgtaatga agcgattgac gtggttactg
    10261 tcaaagaccg gctggaacaa gccaatttgt tagaagatgc cggtggttta agttatctct
    10321 cagacttggc attagcagtt ccgacagcag caaatatcgt ctactatgcg aaaatcgttg
    10381 aagaaaaatc attgctgcgt acattgatcc agacagcaac aggaatcgtt acgaaaggat
    10441 tcgagcaagg agaagaagtt caaacaattt tagatgatgc cgaacgcagt atcttggagg
    10501 tttcggaaaa aagaaatcga agcggctttt tatctattgc tgaagtactg aactccacga
    10561 ttgaaaatat cgatcgacta gctcaaaaca atgaagaaat cacaggtcta ccaactggct
    10621 atcaaggtct ggataaaatg acagcaggtt tccagcccga agaactaatc attcttgctg
    10681 cacgtccagc ggtaggtaaa acagcgtttg cattgaatat tgctcaaaat gtaggaacaa
    10741 aaacagatcg ttctgttgcg atatttagtt tggaaatggg ggcagaatct cttgtaaatc
    10801 ggatgctttg tgcagaaggt tcgattgagg ccagccattt acgtacaggt cagctttcgg
    10861 aagaagaatg gtcgaatctg atcgttgcga tggggagtct ttctcgagca aatattttta
    10921 tcgatgatac accagggatc aaaatctctg aaatccgtgc gaaatgtcgg aaactagcgc
    10981 aagaaaaagg caatctaggc ttgatattga tcgattattt gcagttgatt gaaggtaacg
    11041 gtaaagagaa ccgtcaacaa gaagtatcag atatctctcg ccaactgaaa aaactggcga
    11101 aagagctgaa agtacctgtc attgctcttt cacagttatc tcgtggagta gaacagcgac
    11161 aagacaaacg accagtgcta agtgatattc gtgaatccgg atcaattgaa caagatgcgg
    11221 acatcgtagc cttcttgtat cgagatgatt attatgaaag agaaggcgga gatgacgatg
    11281 atggaaacga gccaaaaaat gacaacgtca tcgaagtcat catcgagaag aaccgaagcg
    11341 gtgcacgagg aacagttgaa ctactgttta tcaaagaata caataagttt tcgtcgcttt
    11401 cgccaagaga agaattttaa agaagaggtt gtgagccttg agcaatcccg agagctctcg
    11461 taagaagatg caaggagtaa ccgcaggaac gactagagta cttaatttaa acagatctcc
    11521 aaagggattg tgacaaaagt cttgtcacag tccctttttc cgaataaacg gtgttcaatc
    11581 agctgaccct tggtattagc tcagcaaacg tcaaaatatg aggaactatt ttgacgtttg
    11641 tttctctaat actcaggtca aaacgctgat cttacgacct cttttcctta aacggtgttc
    11701 tcctgccaac tcctcggtca caagtcacaa tatcatcaaa catgagaagc agttttttga
    11761 aattgctcct tgtgactcag agttaaacgg caggctcaca acctctttct taaacggtgt
    11821 tcaaaagctt gcatctgcgg taatacgccc aatcaaacga aaaatatgag aagctatttt
    11881 tgtttggctg ttcttattac ttgatgcagg acagcttttt cacaacctct gattcacggt
    11941 gttccaaaag tagcttcttc gaaaaataag tcgaaatttc acaaaaattt gagaagcaat
    12001 tttcgtacat tccttcttat ttcttgaagc tgaccacttc tgttacaacc tcttttttaa
    12061 taaccattct aaaaagataa agttcgtttt ttaagcgttt ttataaaata aaaataaaaa
    12121 tcattcgatt ttatttgaaa tatgtatcta ttattgatac aatggttcag gattaaataa
    12181 tatagaacga ggtgttcaca tgtcatctgt agtagtagta ggtacgcaat ggggcgatga
    12241 agggaaagga aaaatcacag actttctcag tgaaaatgct gaagtgattg cacggtacca
    12301 aggtggcgac aatgcagggc acacaattaa gtttaacggt gtgacttata aactgcattt
    12361 gattccttct gggatttttt ataaagacaa gatcagtgtg atcggaaacg gcgttgtagt
    12421 caatcctaaa tcattggtta aagaattagc ttatttacat gaaaaaggtg tagaaacaag
    12481 taatttgcgt atttctgacc gcgcgcacgt aatcttgcct tatcatatca aattggacca
    12541 attacaagaa gatgctaagg gcgaaaataa gattggtaca acgattaaag gaatcggacc
    12601 tgcctatatg gataaagcag ctcgtgtagg tatccgtatt gcagacttat tagataaaga
    12661 aatttttgaa gaacgtcttc gtttgaatct agatgacaaa aaccgtcaat ttgtaaaaat
    12721 gtttgattca gaaccacttg attttgacga tatttttgaa gaatattatg aatacggaca
    12781 acaaatcaaa caatacgtca cagatacttc cgttatcttg aatgatgctt tggatgcagg
    12841 caaacgcgtg ttgtttgaag gcgcacaagg tgtaatgctg gatatcgacc agggaactta
    12901 tccttttgtt acttcttcta acccagtagc cggtggggta acaatcggaa gcggtgtagg
    12961 gccttcaaaa atcaataaag ttgtcggcgt ctgcaaagcc tatacgtcac gtgtcggtga
    13021 tggaccattt ccaactgaat tatttgatga aacaggagaa caaatccgcg aagtaggacg
    13081 agaatacggt acgacaactg gtcgtccgcg tcgtgtaggc tggtttgatt ctgtagttat
    13141 gcgccattct aaacgagttt caggtattac caacctatct ttaaactcga tcgatgtatt
    13201 aagtggattg gaaacagtaa aaatttgtac ggcttatgaa ttagatggcg aactaatcta
    13261 ccactatcca gcaagcttga aagagttgaa ccgctgcaaa ccaatctatg aagaattgcc
    13321 aggctggtca gaagacatta caggatgcaa aacacttgca gaactgccag aaaacgcacg
    13381 taactatgta cgtcgtatct cagagcttgt tggtgtacgt atctcaacat tttctgtcgg
    13441 acctgataga acacaaacaa acatcttaga aagcgtctgg gcgcaaatct aaggataaaa
    13501 acgacgactg aatcaggacg ttcttaccag agcgagtcga agtgtttatc tatacgataa
    13561 taataacttg aagaggtagt gaaagagacg tttagcctta ttgccgatag gctgtctctt
    13621 gaacactgtt tattcagaat aagggactgt gacaaaa
//
LOCUS       DADVXU010000074        13611 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.077, whole genome shotgun sequence.
ACCESSION   DADVXU010000074 DADVXU010000000
VERSION     DADVXU010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.077"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(229..921)
                     /locus_tag="KB953_002035"
     CDS             complement(229..921)
                     /locus_tag="KB953_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBB6867005.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            1006..1182
                     /locus_tag="KB953_002036"
                     /pseudo
     CDS             1006..1182
                     /locus_tag="KB953_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1358..1927)
                     /locus_tag="KB953_002037"
     CDS             complement(1358..1927)
                     /locus_tag="KB953_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBB6867006.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(2001..2324)
                     /locus_tag="KB953_002038"
     CDS             complement(2001..2324)
                     /locus_tag="KB953_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867007.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2478..3290)
                     /locus_tag="KB953_002039"
     CDS             complement(2478..3290)
                     /locus_tag="KB953_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867008.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3643..4491
                     /locus_tag="KB953_002040"
     CDS             3643..4491
                     /locus_tag="KB953_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBB6867009.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4636..5577
                     /locus_tag="KB953_002041"
     CDS             4636..5577
                     /locus_tag="KB953_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HBB6867010.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5567..7738
                     /locus_tag="KB953_002042"
                     /pseudo
     CDS             5567..7738
                     /locus_tag="KB953_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7850..10021)
                     /gene="copB"
                     /locus_tag="KB953_002043"
     CDS             complement(7850..10021)
                     /gene="copB"
                     /locus_tag="KB953_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HBB6867011.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(10041..12227)
                     /locus_tag="KB953_002044"
     CDS             complement(10041..12227)
                     /locus_tag="KB953_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HBB6867012.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12227..12436)
                     /gene="copZ"
                     /locus_tag="KB953_002045"
     CDS             complement(12227..12436)
                     /gene="copZ"
                     /locus_tag="KB953_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HBB6867013.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12449..12889)
                     /locus_tag="KB953_002046"
     CDS             complement(12449..12889)
                     /locus_tag="KB953_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HBB6867014.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12963..13502)
                     /locus_tag="KB953_002047"
     CDS             complement(12963..13502)
                     /locus_tag="KB953_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBB6867015.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3831 a         2843 c         2415 g         4522 t
ORIGIN      
        1 catcctaaaa tataagctat ttgtttactt ctgtagaata tatactttgt tgttagataa
       61 gttgcacatg tttatagaat ctaaataata aatttttatg taccgcccct caaagtgaac
      121 tgaaccccaa aagttgaact atttaatgga ctgtttccga tattctactg gagatagtcc
      181 attaaatagt tcaacttttg gggttcagtt caaagagggg gggatttttt atcgttgtgg
      241 aatatttaaa taccaacgct tgtcatgaaa atcttgtgct ccacccttag tatttccttc
      301 tggatcgttt gtcgcacgca tcatgacgta tactttctta ttagggaaat cacgcatatt
      361 gaaagataca tgatagccaa catttcctaa agtattataa gcttgattta cgtctggtct
      421 ataaatccca tcagctctta ctctagctag ttcttttcca gtattgtagt ccataatgaa
      481 aatatactcg tatttataat tagcaatgtg ccatccagct acatgcaagt ttgcgttttc
      541 gatttctccg aactgatcaa tgtgggcgta atttgttcca tctgtcagcg tagaatttgc
      601 agcacctgct cgagttggat caattactgg tttatcctcc gaagtagttg gattatcatc
      661 cgtaaaacca tgagccaaat cataggctag tttttcttta cttacaccca tttcagaaag
      721 ataaccgtaa ggatctgtat gatcgcccca aatattttgt gttacccata aatgcgattt
      781 gattcctggt tggttataag gagtgtctaa tgttaatgga ataccatatt tcattgctga
      841 atctcttgcc aattcaacgt atactttata gttcttttca aaagtttctt tatcatgtgt
      901 atgttgtaac tcgatttgca caggactttt ggctcacgag gttgtcctac tctacatctt
      961 gctgatagat atgctgaaag gaaaacacca acattatgtt ttgctttttc agctagaacg
     1021 agacaagcag gaataccttt agaagatatc aaaggttttt taggtcataa agatgtttcc
     1081 actacacaag tctatgccca tatttctccc gaagtgaaaa aaagatcaat gaatcaacta
     1141 gaaaactata tagaagagca aataaaaaag cactcaaatt gagtgctttt ttaagctcac
     1201 tatcactaaa actatcacta attttattta taagcgaaca tgtttctatc actgattttt
     1261 taaatctagc gaaaaaccct tatcttaaag gcgttcgttc aattctttgg caaggtcttc
     1321 aaagcctggc ttgcctagaa gtgcgaacat ggttttctta tagctcatgt agaaaaattt
     1381 tcttgatgat gattttatcc atcaattgag attgactgtt ttgctctcct atgatttcag
     1441 ctatgattgt ccgattttgc ggttttcttc ttaactggta gttgattttc ccagtttcca
     1501 tcaactcgct aagcatatcc acgactccgc caaatccggt ctttttcgta ataaaaccta
     1561 gtgctaccat tcgttcatct agattttttg ctgtaatcgt cacaccagta atgagcgagt
     1621 gctgtaatgt ttgggccagc tcttccatcc cttcagcatt ggatacgacg gaagctgttt
     1681 tgttaatagg ttcttcgttt atttcttttg gttctttcct ctccactttc actgtttgac
     1741 gaaggacatc tagttcttgc aagagcgttt cgtattgttc ttgggtcagc atcacgccag
     1801 ctactttttc acgattaaat atatatacgc cagttcccgc gtctcgcgct tgagcaaata
     1861 catccatcgg cgatcgtttc acttctgtga tcgaagatgt gggaacttct aatttcttta
     1921 attccaatgg atttcctcct ttactaatcg ctttttatta tagcacagac tgacttgttt
     1981 tgatcttttt tccaaaggtt ttatcggttt ttgcctaacc agtaattgat atcggtaaga
     2041 agtctttcga taccaaatga aggatggtac agatacgttt tttgatcttc gttatggtct
     2101 agtggaaaaa aatgatcgat gatcactaga tctgcatacc gaggcggctt atttcgagta
     2161 catacatgta tagagatccg ttttcgatgt cttagcgttt tgttcagtac cccagcaagt
     2221 cttcttgctt cttggtgttc atagcgaaaa aaaaagacga ttttcacttc aggagggtgg
     2281 acatctaaat accctcgttc ctggagtgtt ttcagtaaaa gcatccccca ctctactgcg
     2341 gggtgtgcaa gtgcttgttg gatcgctagc cgaaattgat atagtttatc tttggaattg
     2401 ggaaattttt ctaatgtttc ctgataataa cgaacttctt ctatcagccg gtcatggata
     2461 ccaattacgt aacgaagtca ttcttggtaa agtacttttg tcagctttga tttgctttcg
     2521 ctctcaaatt cgctattcag ccaaaacagc gaatcaagct tttcagtcac aatcgtcaat
     2581 ttccgatagt cttgtgtctc ttctatccgt ttccaccgct tttcagtaat ctggttacag
     2641 ctcaagcgca aaattgctag ataatttttt tcttttattg gcaaaatctg ctcctctcta
     2701 aatagcaatt caggaaacaa ttcaaagaat agtgttcgac tgtctcctaa aatctcgatt
     2761 tctgccggat cctttctttg cttcaataat tggatgaaca tctcagaaaa ggactgctct
     2821 ccatcatctg caaaaaaatg cggaaaatct ttcaaaaagc aaaacagcag atatcttact
     2881 tttctttctg aaccaaagat ctgtgctttt ttataagaaa aagaaaggtg gtactcgcca
     2941 aaccactttc tcaatcgctc ttgcgtagaa cgatcgtact tctgctcgta aggttcattc
     3001 aatagcagat ttttcagtaa acaaaacggg ggtgattgga tcagaaaaac aaacaagaga
     3061 taaggttcgc tatatccttg atcataaact aactgaacgg ttttcttatg aagaatcagc
     3121 tgaatgggac agtcgctttc cgccaaacgt ttcagatgcc gatccactgt tttaacatcg
     3181 attttcagtt cttcagctaa atcagctttt cgccagatca aatctttcat tagattgatt
     3241 agtgaaaata aattctgtgc attttttgat aatagttttt gccatctcat cgatcatttc
     3301 tcacctgatt tctaaacata ttgtttgatc aaaatgggtt cactcctaaa aagataaaaa
     3361 actccctttc tatttattta aatacgcaaa tcttcctcat tattttttat tttactcaat
     3421 ttaatcgtat tctcatttca aaacgaatgt actttaaaaa acataataag atcactttgc
     3481 ttgtaggatg tattcccttc cattacaatg aaataaatag cgattttatg agaaggtgat
     3541 gtgaaaatgg aagaaaaaat agaagaatcc aaacaaaaaa tcgatcagtt gatataagaa
     3601 ttagaaaaag cggtcatcgg caaacgttca actatccaat tgatgatcgt ggccctatta
     3661 gcaggtggtc atgtcttatt cgaagatatt cccggtgtag gtaaaacgtt actggtcaaa
     3721 gcactagcaa aagctgtaca gggaaccttc agcagagtcc aatgtactcc tgatctgctg
     3781 cctagtgata ttcttggttt ttccgtatac aattcacaat caaaagagtt tgaatttcgt
     3841 ccgggaccga tctttaccac gatcttgtta gcagatgaga tcaatcgtac gactcctcgt
     3901 actcagtcag cacttttaga agcaatggcc gagaatcatg caacgatcga caacaataca
     3961 taccctctag acaatcattt ttttgtctta gctacgcaaa atccaatcga gtacgaagga
     4021 acatatcctt tgccggaagc acagctcgat cgttttttat tccgactaca aatcggttat
     4081 ccttcttttg atgacgaact tttgctactt ttggataaat tcgaaaaagt gttggataac
     4141 ttaaatgaag tattgaacag ttatgaaata gaagaaataa aaacaaatgt ggcgaatgtc
     4201 tttgtcagtc ccgaagtggc atcttatgcc ttgcagttag tgtcagcaac aagaagtcat
     4261 gctgccatcc agcttggcat cagtccacga ggaagccttt cctttattca ggcagcaaaa
     4321 gcttatgctt taatccatgg gagaaattac gtcacgccca aagatttgca ggatttagtt
     4381 ccttacgtgt tttcccatcg cctgattttt tacgatcgtt cattgaagga agacgaaaag
     4441 aaacaaatca ttcatactct tgttgcacaa attcctattc ctgtgaggta gattgaggta
     4501 gacaatggaa aaaatcaaag attatttcat taattttgtg ttatttctgg tatatttcat
     4561 cattgtgctt tttgctcttg tattcaacaa ttctttaggc tggttcttgt tggccttttt
     4621 tactctttat gtatcatgga gcttgatttc attaagcagc tttctaaaga aactttcggt
     4681 tacttgtaca ttgcagccac tcgtttatcg caaaaaaagt atgcaggtta ttttttccat
     4741 agcaaaaaag agaacttttt ctttacctct ccctcgttta caaatcggct tcccagcaat
     4801 ctttgcagaa aaaaacaaga gattttgatt cttacaaata aaccgcaaga agtccaattg
     4861 ctttggcagc caaaagaaag aggatgccat gaaacattaa ctgtcatttt tacagcctat
     4921 gatagtctag gacttttcag aaaaagtgca aaaagagaga tctcttttcc tgtgactgtt
     4981 ctgcctgctt tttgtaagat tcaagcagaa aaattacaga gagttctgga aaaaaaacag
     5041 cagctgaacg cgtaccaaag aggtcttgat tttcgggaac atcgcgctta tcgtccaggt
     5101 gattctttca accgaatcga ttggaaattg tctgctcata ctcaagagtg gttataccgt
     5161 gaatacgatt atcaagaaga agaatgttct cctctttttt gcttttgggg ttcgcccagt
     5221 ccaaaatttg aacctctgct agacttgtat ttttctttat ggcatttgag aaaagaaaag
     5281 cagccggaac ttctgatatt aggcaaccgc gcctttcatg gcaaagaccc cggtcatacc
     5341 tattttgctt ctttggaagc tggcgatgag aaagcttttt tcgcacactt gaaaaaatat
     5401 gcaaaaaaac aaatcgtcct ctttgttcct gagaattctc ctgaagtctc agaagctatt
     5461 gaaacattgt caaaagatcg aacggtttat cttgtttatt tcgtagaaaa acaactcatg
     5521 gtccaagaaa aagacaagat atgttcattg ccaggaggtg aatggattga cgattaaacg
     5581 ggaaaaatgg ctgcgtggcg tttactcctt tgtgatacta agtgtaggtg ctccgatatt
     5641 tgtagtagct aatcaattgc aaaacagcta ccttcttatc ctaactattg gtgtcctatg
     5701 ccttattgtc tgctgtattc ctaaaaaatg ggtacgtttg ctgctttctt tgccagtaat
     5761 gataggttgt ttgtttttat attttccttc gaagacattt tccatattat ggatcttaca
     5821 gtttttttcg tcagtcacag aggaattctt ccatctatcc caaggtaaac tttattattt
     5881 gccagaaaag acagcttttt ttctcataat gttgctgatc tatttattta ttacattgac
     5941 cgtggattac gactattggt acgcaccctt tctttctttg atggcttact tcatgatgct
     6001 taccgttttt cggaaaaaag atttactgta cgaaatgatt gcggtagtaa ctgttttgtt
     6061 cgtgtatctg gcagcaaggc aatttttctc catccggttg ttgtcgggaa acactcgatt
     6121 ccagtcattg actacagttc tgcttatttg tttcttaact tttagcgcta tcttgccttt
     6181 atatcttccg aaagtacacc aatctctcga ggaaacgagt gaaccgatcc gagaatatct
     6241 taatgatgaa gggctttatg acacactcca tgagtatcaa ttaaccggaa gtgcaagaac
     6301 aggcttcagt gagaatgatg aacagcttgg tggaccttta tatgataatg gcgaagttgt
     6361 ttttacagcg aaccaaaaag gaaatggcta ttggaagatc gaaaataaaa atcattacac
     6421 cggttctgga tggatagaag atcaggacgc acccatccaa ccaatccaag aattccctgt
     6481 tgacatcacg agctaccaaa ccgttataga agacaaagaa aacattacat tgaccctaaa
     6541 aaaacaagtt ttcttacctt atccttatgg aaatactacc ttgactgatt tttcattagc
     6601 tagtggacaa acgctttact atttgcctga aagccagcga ttcattcctg tcccgaatga
     6661 atcgacacgt tctgcaactt tacaaacaga aaggttagtt tttacgagag aagaattaaa
     6721 aagcagtcag ttaaaagctg atgatttaga aaatcagcag acaaatcttc aattgccaaa
     6781 aagtttgcct aaacgtattg aggacttggc ggtcgaattg accaaaaatg aagcaacttt
     6841 aattcgtaaa gtcgaagctg tctaacagta tttaaaaaca agcggaaatt tccgctattc
     6901 caaaacggat accgggcata ctccaaaaga acacgattat gtggattatt tcctctttga
     6961 ttcaaaagtc ggctattgtg acaacttttc aacttctatg gtcgtccttc ttcgaacact
     7021 tggtatccca gctcgctgga caaaaggttt tacaaatggg ctcagaacag gcacagataa
     7081 tcaagggaat gaggaataca gcattctcaa tagtcatgct cattcttggc cagaggtata
     7141 ctttcctgga tttggctggc ttccatttga accaacccct tcatttgcca atccaatggg
     7201 ttcacaacca gttgctgaaa caaccgaaac cgtccagcct gctatcgaac aaccaaatga
     7261 aagcgaaaat atggcagaaa ctatttccag tgagagatct gaggtcattc catcagaaga
     7321 aacaacactc acaccagctt ataaaatcgt cttattcagc cttggagtgt gtttatccgt
     7381 cttgttgttc tttctattgt tattcagaaa atctctgcgc tggacaagtt atcttatcta
     7441 tcttcatttt tttgcttcta ctaatacgat tgcatatcaa aaacttctta accctttgga
     7501 aacattggta ccgcgcaaac ccgcagaatc tttgcaggaa tatagcctgc gaatagaaca
     7561 agaattcaag ctagatctgc gtttcattcg attaactact caatatgaag cagaaatata
     7621 tggacgacaa gcagatccgg taaaattcga taaaaaaacc tatttagaaa taacgcatat
     7681 aataagaaaa atggtataca aaaaaattct gacaaaaggg agccgcttca aaaaataaga
     7741 taaaatttct ggagaatcta cttctggaat gccgagaacc agctgattga gtatcgatta
     7801 ctcgaaaaaa aggttgtgac acgttttttg tcacaacctt ttttcattat tatctgattt
     7861 tcaatgtcaa agcatttaga gctacaacta ccgtacttaa cgacatcaag acagcaccaa
     7921 ctgctggact taatatgata ccaattggcg caagaattcc ggctgctagc ggaattgcaa
     7981 gaatattgta acctgccccc caccagaggt tctggatcat tttctttcgc gtttgcttgg
     8041 ctagatcaag aaaatgcaaa atatcttttg gatcgctgtt tgtcaatacg acatccgcag
     8101 aatcgattgc gatatctgtc cctgcaccga ttgccatccc gattgaagca cgggctaaac
     8161 ttggcgcatc attgatccca tcacccacca tgattacttt tttcccttgg tcaaggtatt
     8221 tctggacaac agcttcttta tcatctggta ataaaccgcc ataatattct tcgatcccta
     8281 aataatctgc tactgcttgg gcagcgtttt tattgtcacc agtaagcatg acaggagtga
     8341 tttttctttc tttcaatgtt cggataaatt cttttgcttc tgtttttact acgtcaccta
     8401 aagcaacgat tgcagctaga tgattttcta aaatcaggaa gctgatggta tttccttgtt
     8461 cttggtaagg tttcaataat gcttgttcta cgtttaatcc gagacgctca acttcttttt
     8521 cattaactat tttcacttgt tgttttttga ctgttgcctc tagcccgact cctgaaagat
     8581 tttttagatt ttcagcttga taaggcttga tttcatgttc tttcaaataa ttcataatgc
     8641 caacagccaa tggatgattg gcattcgctt ccaaggctcc tagatattga aggatttcat
     8701 ttttattgaa cgcatcatca agtacttcga cacccgttac agtaaatttt ccttctgtta
     8761 gtgttcctgt tttgtccagc atgatcacat ctagatcatt ggcttgctcc agtgcattgc
     8821 ggtttttcaa aagtaatccg tttttagctg ctattgatgt ggaacgagca acaactaatg
     8881 ggatagctaa acctagagca tgaggacagg cgatgataaa gactgttacc atacgttcta
     8941 atgcattagg aagatctgtc aagaacaacc aggcgatgaa tgccaggata cccacaatta
     9001 atgcgacata aaacagccat tttgccactt tgtcagataa tgactctagc ttggattttt
     9061 ctccttgtgc ttttcgcacc atttccataa ccttcgctag ataaccattt tcaccagttc
     9121 cagttacttc gatttcgatc gttccatccc cattgataga accgccaatc actttgtcat
     9181 tggcttgttt tttgattcct cttgattcgc cagtcacagc tgattcgtca acaattgttt
     9241 cacctttcaa gattacacca tctgttggca ttttatcacc agaccggacg atcaaacgat
     9301 caccttcgtt tacttctttc aatgaaactg tctcttcttt tccatctttc gtcaagcgtt
     9361 tcactgattc tggcagtaat tctgctagtt tttgtaaagc attgctggca ttcgacacag
     9421 cattcatttc aatccagtga cctagtagca taataacgat caaagtagcc aattcccaga
     9481 agaaatccat gacgtgtgta tgtggattga gcagatttgc gataaacgaa tagacactat
     9541 aaatatatgc aactgtaatc cccatcgcga tcagtgtcat catagcagga cttttttgtt
     9601 tcaattccat cttagctcca cttaaaaatg gctgaccgcc atagataaat agtatagttg
     9661 ctaagatcaa tacgagccaa tcagaaccag gaaacgttac ttgaaatgga aattccattc
     9721 ccatcattgg tgaaaaaaga atgattggga tagctaggat aagcgatagc caaaattttt
     9781 gtttgaaatt ccccatgtgc atggaatgat ccatgccgcc gtgttcgtga tccatgtgca
     9841 tcgaatggtc catttgtttg ctgtttgcta tatctccatg gttatgtccc atgtgcatgg
     9901 aatgatccat ctgactatgg tcatgtttca tctccatctg tttttcatga gattctgtac
     9961 tttctttgtt tactacttct tttttatgct catgtttttt ctccatatcg ttcccattca
    10021 tttttactcc atcccttctc ttactttttc atgtgtcgat ttaagcacag ggagttcaac
    10081 aatacactga cggaactaaa cgccatcgct cctcctgcaa tgattggatt caaaaagcct
    10141 agtgcggcaa aaggaatacc aatcgtatta tagacaaaag cccagaaaag attttgtttg
    10201 atttttctca atgtcaacct agacaactcg attgtttgtg caatggatgc caaactgcta
    10261 ttcatcagcg tcacatcggc tgtttccatc gcaatgtccg ttccacttcc cattgccata
    10321 ccgacatcag ctaatgctaa ggccggtgca tcattgattc catcgcctgc cataccgact
    10381 tttttgccat ctttctgaag tttttctacg taggctgctt tatcctctgg aagtacttca
    10441 gcaaagatat gctttggatc aataccaact tgtttaccaa tcgtttcggc tgccagtttg
    10501 ttgtctcctg tcagcataaa aacatctaat cctttattct ggagctgctt gattgcttgt
    10561 ttgacttcta gtttgatttg atcagccacg gcaatcaacc cgatcacttg cttttcatca
    10621 gctaaaaaca tgactgtttt tccctgctgt tctaattcta acgcgtgttc ttggtattca
    10681 tcaaatgata ggttcaattc aattaatcgt ttacgtgtac ccgcaaaata gcgtactccg
    10741 ttaatcgtac cgctgattcc agcacctggg tgggctgtaa aatcaatcat atcgtatgtt
    10801 gctgcttctt gcagttttcc gtattccacg attgctttac ctagtggatg ttcagaagct
    10861 tgttctaaag tataaaaaat agataggact tctttagaac cgactagatc tgtaactttg
    10921 ggctttcctt ctgtaatcgt tccagtttta tccaaaacaa tgctgtctaa gtgagcagct
    10981 gcttctaatg cttctccgcc tttaattaaa attccatttc gtgcaccaac acccgttcct
    11041 accataatag ctgttggagt cgctaaacct aatgcacatg gacacgcaat gactaataca
    11101 gagacactat ggatcaatgc gagttgccaa tcaccagtga tcaaacctgt agctatgagc
    11161 gtgataaacg cgatgactaa tacgatcgga acaaaaatcc ctgagatccg atctgcgatt
    11221 ttttggattg gggctttgct cccttgagca tcttccacca tttgaatgat ttgcgctaat
    11281 actgtctgct tgccgacttg tgtaacttcc atgtgaagta gaccattcgt attgattgtc
    11341 ccgccaaaga gcgtgtcatc cggattcttt tctacaggaa gattttcacc agtaagcata
    11401 ctttcatcta tcgcactgct tcctgaaata attcgaccat ctgctggaat ctgctcacca
    11461 ggacggatga ctagttgatc accaacgact acttcttcaa tcgctaacgt ttcttctttc
    11521 ccgtttctta tcacttgggc ggtttttgtt tgaagagaca tcaattgttt gattgcatta
    11581 cctgttttcg tttttgctgt atgttccaaa tatttgccga gcaaaatcag cgtgatgatc
    11641 atactgctgc tttcaaaata aagatcttgg gggtgtccac ggaaaaatcc gttgtaaatg
    11701 ctcaatgcaa aggcagccga tgttccaatt gctaccaata catccatatt tggtgctttc
    11761 gtcttgattg catggtacgc acctttataa aaacgagcac cgacataaaa ttggacagga
    11821 gcagacagca ctaactggac gattggaaaa tgaaaaaagt gaacaatcgc cgcatggctt
    11881 cctaacatca tagcaatcat tgacaacatc aacggcaaag tcaaaaccgt actcaatata
    11941 agatcgaata gcattttttt caaatacgct tgcttttctt ctgcaatttt ttgtttgtgg
    12001 gcttcatcgt acaaaattgc accatagccg atgttttcta cgctttggat taatttttca
    12061 gtggtcgtcc cttcatattt gacacttgct ttttcagtgg ccaagttgac tgtggcattg
    12121 accacaccag gttgtccgtt cagctctttt tcgatcctag cagaacaatt tgcacaagtc
    12181 atacctgtaa tgacgaacgt atccattttg gtttctgctg ttgccatcaa atcacctctg
    12241 ctttataacc caatccattg attgcttgac aaatcttttc ggattgaact tttgtttcat
    12301 caaacttgac cgtaccgttt gcttttttca aatttacttt cactttttgc acgccgtcaa
    12361 gtgcgcttac tgtttcttct actcttgctg cacaatgatt gcagctcata ccttcgattg
    12421 aaaattgttg tttcattttt tcttcctcct attgatgttg tttacattca cattgacctg
    12481 ggatacagtt acattcgatc gtatctactg gttgcttttg ttccagttgt ttcaaaatct
    12541 cattgatatc ttcttgagtc aaagtggctt cttctactag atccgcaatc gtctctccaa
    12601 tcctttttgc acagatatgg gaaaaaagat tttccgtcgc actgcgaact gtttccatct
    12661 ccgagacagc tggataataa atgaattttt ttccttcttg ttctgtccag atcacttctt
    12721 tttttaccaa acgacccagc aaagttttga tcgttgctgg tttccaatcc atcgaatcag
    12781 ctaagatctg cgtgatttct tgggctgtcg tctgtccaag tgtccaaata acacgcatga
    12841 tctcccactc tgaatcactg atctttactg gcgttttgac tttttccatt tatttcacac
    12901 ctttcgttta caaatgtaat cattatctta tgaatacaga ttacacttgt aaacaaaaac
    12961 tgtcaattat ttttgctctc tattttcaat ttagttaaaa attcttgttt ctctcgtctt
    13021 gttttaagga tgatcagttt atttcttgca ttagctactt ggatcagctg ctgtattttc
    13081 ggttcattgt tttcttcaaa atgccagata aaagataaaa attctaagta ttccctgttt
    13141 agtttctccg aaaatgattc aggcatatct ggccgagatt ttttgtttat ctttcttttt
    13201 agagaacgcc aaactactcg gtagatggcg agataacgcg gcactttaag ccaaatgatc
    13261 ttatctgctt ctttaatcct ttcctctaat gtagaagtat aatttccttc aacgatccat
    13321 gacggattag ataccataaa caattgctgt tcttgcaaaa accacttttt tgcctgaata
    13381 ctgtagtctg ttgtatgcca taaaaggtct agcggcagta gcggaaagtc ggtgatttct
    13441 ttgatcttcc ttgcgagggt cgattttccc gacccacttt gtccaatcac gatgatcttc
    13501 attcttctcc ctcacttctc tttggaattt ggttcgatca taccacttat aacgccgcaa
    13561 aaaaacaagg gactgtgaca aaagtcgtgt caaagtccct tgtttcgaat a
//
LOCUS       DADVXU010000075        13266 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.078, whole genome shotgun sequence.
ACCESSION   DADVXU010000075 DADVXU010000000
VERSION     DADVXU010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.078"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(198..270)
                     /locus_tag="KB953_002048"
     tRNA            complement(198..270)
                     /locus_tag="KB953_002048"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(235..237),aa:Lys,seq:ctt)
     gene            651..1220
                     /locus_tag="KB953_002049"
     CDS             651..1220
                     /locus_tag="KB953_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HBB6867016.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1207..2067
                     /locus_tag="KB953_002050"
     CDS             1207..2067
                     /locus_tag="KB953_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBB6867017.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2257..2961
                     /locus_tag="KB953_002051"
     CDS             2257..2961
                     /locus_tag="KB953_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBB6867018.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2966..4801
                     /gene="walK"
                     /locus_tag="KB953_002052"
     CDS             2966..4801
                     /gene="walK"
                     /locus_tag="KB953_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HBB6867019.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4798..6114
                     /locus_tag="KB953_002053"
     CDS             4798..6114
                     /locus_tag="KB953_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867020.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            6115..6984
                     /locus_tag="KB953_002054"
     CDS             6115..6984
                     /locus_tag="KB953_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HBB6867021.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            7039..7848
                     /locus_tag="KB953_002055"
     CDS             7039..7848
                     /locus_tag="KB953_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBB6867022.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7951..8580)
                     /locus_tag="KB953_002056"
     CDS             complement(7951..8580)
                     /locus_tag="KB953_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HBB6867023.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8750..10042
                     /locus_tag="KB953_002057"
     CDS             8750..10042
                     /locus_tag="KB953_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBB6867024.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(10118..10780)
                     /locus_tag="KB953_002058"
     CDS             complement(10118..10780)
                     /locus_tag="KB953_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBB6867025.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            11005..11289
                     /gene="groES"
                     /locus_tag="KB953_002059"
     CDS             11005..11289
                     /gene="groES"
                     /locus_tag="KB953_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBB6867026.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11340..12965
                     /gene="groL"
                     /locus_tag="KB953_002060"
     CDS             11340..12965
                     /gene="groL"
                     /locus_tag="KB953_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBB6867027.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4418 a         2286 c         2854 g         3708 t
ORIGIN      
        1 cccctcaaaa tggaggggaa gtacatactg tgataatcta caggtttatt gactgattag
       61 gatgttatta aaaaataaaa tactaaaatg catacccgaa tttcataccc gaaaaataaa
      121 aaatgccacc acgtacactc ttttcgaaac tacctaagta gccaagaatg tactggtgac
      181 gtgggtttgg cacccaatgg gccgtgaggg gctcgaaccc gcgacccgct gattaagagt
      241 cagctgctct accaactgag ctaacggccc gtacaaaaag tacttaataa tagtagcact
      301 aagaatttta aaatgcaaga ctacatttaa agaaaatcag aaaaatcttt acttccttat
      361 aggaaatcat tcatagatta ttcacatttg ctcgctatta tataagcata ccaacaaaca
      421 aagttcttgc aagagaacaa accctaacac ttttttcatt ttttactcct ttcccgccgt
      481 ctccccggcg ggtctcctgt tcttaacgat aaaagacaag aaggcttagc cttcttgttt
      541 tttgttttca tggaatcccg ctaatcaaat cacctagtta aagcaactag attgaatgtt
      601 tatcataagt ctgatatcat gtttatgaaa tgaaaaaagg agcggttatg atggaaaaaa
      661 tgcgtcagca attaatggag tgggggaagg aactagaaac attccgtctg cctcactggg
      721 aagaactccc tgacttagaa ctttatatgg accaagtcag aacgttagtt gatcgttatc
      781 tttcacctgt gatacaagga gaaaagcatc cattgcttac ctcttctatg gtaaacaatt
      841 acgttaaact aggtttgata ccagcaccag ttaaaaaaag atataacaaa gaacacgtag
      901 catttttact tgcaatcact acgttgaaac aagtattgac tattcctgaa atcaaagaag
      961 ggatattgtt ccaaggaaaa acagtaggaa ttcgtgaagc ctataatcta ttttgcgatg
     1021 aacaagaagc agctgtgtgg atggtcagcc aattagcaca aggtaaatct catccgcaaa
     1081 aattcgacca ggcaacccct gtagaatata ttgctgtaag agcggcaact ttatcgtttg
     1141 caatgaaact tcttgcggaa aaaacgattg ttctagaaac cgaatattta aaagaggaga
     1201 aaacaaatga aaaacaataa aatcgcaatt ttagtagatt caggtacgga tgtgccagaa
     1261 gaattgataa aaaaatacga gatgtatatg atcccattga aaattattta taaagatcag
     1321 gtatatacag ataaaattga cattacctct gaagagatct atcaacgttt gccagaggaa
     1381 atccctggta catctttgcc ggatggtgag tcgatcacag aaatctttga gcaaatcaaa
     1441 aaagacggtt atgaaaaggt acttgcggta acaatttcca gtggattgag cgggacatat
     1501 aatgtggttc gtttaattgc acaacagcaa gaagaattgg aagcatatgt actagatacg
     1561 aaaaatattg gtattggtgc aggtttttct gcgatccaag cagcaaaatg gctggaagaa
     1621 ggagtagaat gggatcaact gatcagtaat ctgaatgagt tagtcgaaag aacaaaagtt
     1681 ttctttaatg ttgcgacatt ggaatatctt caaaaaggtg gacggatcgg tttagttgct
     1741 tctattgtag gaaccgcttt aaagcttaac ccaatcatct catgtaatga agaagggatt
     1801 tattacacag taggaaaagc taggggacgt aaaaaaagtt tggataaaac ggttgcttac
     1861 gtcaaagaaa aaatcggtaa tgcaaaggtc ttcaacttag ctgttgccca tggggatgcg
     1921 aaagaagaag caattgaaat ggaagcgaga cttaaaagag agttcccaca agcaaaccaa
     1981 atctactttg gccagatttc tccggcatta gtagtccaca ctggtccggg attattgggt
     2041 gtcggtgtac aagttttaga aggataaatc aaaaaaagga aacactgagg tgaaaatcag
     2101 tgttttcctt ttttattgaa gttagaacaa aaaataataa agtattttgc actttcattt
     2161 taattttctc ttttgtttta taggcaaaga tgaaacaaaa gcagaaatac ggtataatga
     2221 gaaggtatga caataatata taagaggtgg ctctttatga aaaaagtttt agttgtcgat
     2281 gatgagaagc cgatttcgga tatcgtcaaa ttcaatttag ccaaagaagg ctatgatgtt
     2341 tatactgcat atgatgggga agaagccttg gaaaaagttg ctgaggtaga acctgatttg
     2401 attttacttg atttgatgct acctaaaatg gacggcttag aagttgctcg tgaggtacgc
     2461 aaaacttatg atatgccgat cattatggta acagccaaag attctgaaat tgataaagtc
     2521 ctaggcttag agttaggcgc cgatgattac gtgaccaagc cattttccaa ccgagaatta
     2581 gttgcacgtg tcaaagcaaa tcttcgtcgc ggtgccacgg ctgcgaagga accagaagag
     2641 gcagcacctg cggaattgac gattggtgat ttgacgatcc atccagaagc ttacatggtg
     2701 acgaaacgag gagaaacaat cgagttaaca catcgtgaat tcgaactact cttttacttg
     2761 gcgaagcacc ttggacaagt gatgacaaga gaacatttat tacaaacggt ttggggttat
     2821 gattattttg gagatgtacg aacagtggat gtaactgtac gtcgtttgcg tgaaaagatc
     2881 gaagataatc caagccatcc aaattattta gtgacacgtc gtggtgtcgg ttactatttg
     2941 cgtaacccag aacaggagta attggatgaa aagaaaagtc cgcttttttc gatcagtcaa
     3001 ttttaagatc gctattacct ttattctgat tttattgatc tcgatcgaaa ttattggtgc
     3061 ctactttata cgtggtttag aacgttccac gatcaataca tttattaaag atatgaatca
     3121 aacggtggaa tcattagcaa caacaatcag cccggaattg aatcgaaaag ataatgcaga
     3181 tgatgaagag gtaaatgcga atatcaaaag atttattgaa aacagtgcga ctagcgatat
     3241 catcgaaata cgggtcgttg acgaaaaagg gatcatccga ggaacaaccg atgtcaatga
     3301 acaaagcgct gtgggaaaga aaaatgatta tgttgatatc aatgatttca cgactaaacg
     3361 ttatgttgct ttagataacg acgataaacg ggtgaatatc aatgttcaac cgatcctgtc
     3421 cccgaccggc gatgccgtta tcggggctct ttacgtcaaa agtaatatcg aacaaaaata
     3481 ttcggaaatc aataacacag cagtcatttt ctttactgcc tcactaattg cggcatttat
     3541 ttcaatgatc gtatctgttt tagttgctcg ttcgattaca cagccgatcg gtgaaatgag
     3601 agaacaagcc attcgaatcg ctagaggaga ctacagtcgg aaagtcaaag tgcatggaca
     3661 agatgaactt ggacagctgg ctgatacctt caatcagtta gctgaacgta ttgaagaagc
     3721 tcaagataca atggaagctg aacgcaatcg tttggatagc gtgctatccc atatgacaga
     3781 cggcgtcatt gcgacagatc gcaggggtaa agtcattacg atcaatgata tggctgtttc
     3841 cttgctagat atcaaaaacg aggaagcaat cggacaatcg attttgacac ttttagatat
     3901 cgaagaagaa tatacgttgc ggaaattatt agaagatcca aacgaaatgg tacttgaccg
     3961 ttcaaccagt acattggaaa gtgatcagat gatcttgcga gtagactttt ctatgatccg
     4021 tcgcgaatca ggttttatca gcggattagt agctgtcttg catgatgtga cagaacaaga
     4081 aaaaacagaa cgggaacgtc gtgaattcgt ctctaatgtt tcccatgaac ttcgtacacc
     4141 gttgacaagt atgcgtagtt atatcgaagc attgagcgaa ggtgcgtgga aagacgagga
     4201 agtagcacca aattttttga aggtaacgct agatgaaaca gaccgaatga ttcgaatgat
     4261 caatgacctc ttaaatctat ctcgcatgga tacaggtaat acccagcttc aattagaata
     4321 tgtcaatttc aacgagttag tgaattttgt tcttgaccgg tttgatatga tggtgggaaa
     4381 tcaggaaaaa aattataaga tccgtcgtga atttacgcaa agagatcttt gggtcgaagt
     4441 agatacagat aaaatcatcc aagtagtcga taatatcatg aataacgcga tcaagtattc
     4501 accagatggg ggaacgatta cttgtcgctt attagaaacg cataataacg tggttttaag
     4561 tatcaccgat caaggattgg ggattcctaa aaaagatctg aaccgagtgt tcgagcggtt
     4621 ctatcgagta gataaagcac gtgcaagagc acaaggtggg acaggacttg gattagctat
     4681 ttctagagaa gtgattaagg cgcatcgagg cgctatttgg gcagagagca aggaaggaaa
     4741 agggtcgact ttttatatct cgctgccata tgaaccttat gaggaggaat ggtgggaatg
     4801 agaatctctg aaaaagtcgt tcgtatagga ctcattttga tggttgcgtt aagcatatac
     4861 ttctcttatg caatctggct gagcccagca ggaaaaagtt caatcaacgt agatgagaca
     4921 aacactcaaa tgatcgagtc tcaaagcaat cgaaaaactt ccgaagtctt tcttccgctt
     4981 catgttacat ggcaccattc gggaatgatc caagaaacca acagtgaaaa tctcgtgtac
     5041 cgattgcaat tgatcatcga taaagcaaga tttggaaagc tttctgaagt agtttcagga
     5101 gacaaatcac agtttgaaca ggtgaaaaat ctgaactctg gttttgagtt ggcctataat
     5161 gcgccttttt tgctaagaga atacaaggaa gcatttgatt tagatttaga tgtgtcgaac
     5221 ttggcaaaag aaagcgaaaa tatgtatttt actagaatac aattcgatca aggaaataaa
     5281 aaaatccgat ttttgaatta ttcgaaccat ctcgtatatg aagcaaatat ttcgttgaat
     5341 tggacagata tcaataaaga attaacagca aaagacacaa aatggacaga aatgacaaca
     5401 gaaccatcga cgatcgacac gcaatatgct acgaaacatt cgatcaaact gaaaaagtat
     5461 agttatatcc tgtcacagca accctataca ctttttagaa atgctttttt ccagaaacca
     5521 gatagcgtga aaaataatga tgaaagcaat gaattgattt tttacggtgg gaatgaaaca
     5581 atgtctatcc attcggaaac gcaagtagtt gattttcgtg gagagctgcc agagaaaaaa
     5641 gaaaatgcta gtgtattttc tgaaagtttc ccctatatta gcaaattagg cagttcttta
     5701 gggaacctca gatattttga ccgcagtgac aatacgatcg attatcggat attcgtggaa
     5761 ggcttcccgg tttttgggac agacagcaga ggacagattg gtgtggaagt tggcggtgaa
     5821 acgaatggtt ctgtgcaagt gaatatccaa accagtttga atacgattca agtgccgata
     5881 ccttcagacg aagaagtaga acttgcaagt acgaatgaaa tcatccagca gttaaaagct
     5941 aagggagcag atagcaacaa gattggttca gtgatcgttg gctatacatg gcaaaatgtc
     6001 aaagaaacga atcgtgtcgt tgatctctta cctgaatggt atgtgaaata taaagataac
     6061 tggtattcag ctaacgacct tctggaaaaa ttacctggat tggaggcgaa ttaattggat
     6121 ttcaaaaaaa tcgaatggat atttttcgtc gcttttttag ggttgaatgt gtttttattc
     6181 agcagttatc atgacgcggt ataccaagaa aacaatgtca ttcgctctaa tcagactgaa
     6241 tcaatccaac aacgtctggc tagcgacgat atcacttaca cggggaagat ttcgtctgag
     6301 aacaagcaag gctattatct tagcgcggag cagaccaatc ttagcacttc attagcgaat
     6361 tatcgcaagg ctggtaaaag cggcttgctg gcaagtggtg tgtttttaga cggtgatgtt
     6421 ttgacgaaat ctttatccag ttcagatgct gaaaagaacg tgatcgatcc agataaaatt
     6481 agtagttcct tggaagattt tttatctaac aaagacaata ttttattcgg taatgattat
     6541 gtgtacacga aaaacttttc tcatttagaa gaagaaccac tagaaatcca agcaagccaa
     6601 gagtatgaag gcatcccatt caatgatgat acagcaaaaa tcgttctttc tttagaaaaa
     6661 aacgataatg attatagcat cacgaaatac atgcagacgc atttatctga tatcgagcag
     6721 ctgcgcgaaa agacggaact gtatacggaa gaggaagcaa tcaatacatt gtacatcaat
     6781 aacaaaatac caagaggatc aaaaatttta tggcgtcagc tagcttattc ctgtattctg
     6841 aaagtccggg aaaaaaatgt gtatgttcct gtttggcatg tagcgatcga aacaagcgat
     6901 aaagtggtcc aaattgagag tgtcaacgct ttcagcaata cgatcgtgac aaacaatact
     6961 gtaccaaaag tggaagatca ataaaaaagc gagtataatg aaaaaagttt acaaagtaag
     7021 aagggaagta ggagtcaaat ggctgaagaa aaaccagctt ttaaaatcag tatcctagca
     7081 agtggaagtt cgggtaattc actttatatc gaaagtgaaa aaaaacggct tttggtcgat
     7141 gcaggactta gcggtaaaaa aatcacttcc ttgatggctc aaattgatcg gacaccggac
     7201 caattagatg gaatacttgt gacgcatgaa catcgagatc atatacatgg cgtgggtgtc
     7261 ttggctcgta agtatcattt ggatgttttt gccaatcagc agacttggga agcgatggaa
     7321 ccactgattg gaaatgtacc agtggaacaa aaacatctat ttgagatggg gaaagtcaga
     7381 acattcggcg atatcgatat tgagagtttc ggcgtctccc atgacgcagc ggctccgcaa
     7441 ttttatcgtt tccataaaga aaatcggtca ttcgtcatgt tgacggacac cggatattgc
     7501 agtgattata tgcgaggtgt cattgaaaat gccgatggtt atttgatgga aagtaatcat
     7561 gatcttgaga tgttacgcat gggaccttat ccgtggaatt taaaacagcg tattttagga
     7621 gatagaggac atctttccaa cgaagacggt gcacttgtca tgactgatgt gatcggtaat
     7681 cggaccaaac gaatctatct tggccatctt agcaaggaaa acaatatgaa agaacttgct
     7741 catttaacaa tggaaaatgt tttgaaagaa aaagattttg gcgttggaca tgattttgaa
     7801 atctatgata cagatccaga tgaagcaact gagctatttt cgatttaaac agttcaatca
     7861 ataaaaatat aaaaagcaga agataaaatc aatcagggtt ggttttatct tctattttta
     7921 tttttttagc atattaaaaa aataaccgta ttatcgactg actacactga tgtgctgctt
     7981 ttcatgtttc tcatccagta tctcatctac taatgcaatc gcgtaatctg ccatgctgat
     8041 cgtactgtcg ccagcagcgt ttctttgtaa ataatcatca cttaacaaat aagtacctgt
     8101 acgtttgcca tgtggatcaa ataaagaagc tggactgata taagtccaat gtagattttt
     8161 agattgtttc aattttaaca atccttcgta catattgaaa gctgttggat agaagggtgc
     8221 atttttaggt acttggtcaa tcatacgagt cgtcttttct gtatctaaga ataacgaaga
     8281 ggcaccccca acgacaatta agcgtgtatc ggttccttca agcatctcaa tcaaatggga
     8341 caaagaagtt tgatgcattt cttcttttcc tttgggagcg ttgaaagcat caacgacaac
     8401 atcaaaagat gcaagatcag tagttgtcaa ctcaaataaa tccttttgta taaacggaac
     8461 atctgatggc aatttttctg gatgacgcac gattgccgtc acgtctaaat ctctggacaa
     8521 tgcttcgtct aaaataaatg aacctgcatg gccagtagct ccaatgagtg caattttcat
     8581 aaaaaacctc cttttttatt cctgtccatt gtaagcagtt gctgaatatt cgtcaaagaa
     8641 tccgattttt gaaaagctca gtgcgcattc ctttcatggt tcgtgatcat tgggtatagt
     8701 taaattaaga aaactgattg ataaaaatag aagttgaagg agataagaaa tgaaagagtg
     8761 gttagatcgt gcctttttac caatgataac atctgttgtg aatcttagac ccttaactgc
     8821 tttgcgtaat ggaatgactt acacgatccc aataattatt gttggctcgc tgtttttctt
     8881 atttgctcat tttcctgtgg attacatcgc tcagtggata gacaaaacag gattttctat
     8941 gtattttaca caagtatacc aggctacatt tgatgtgata gccttttgga tcgtttttgc
     9001 cattgcatac gcttatgcaa aagaagaaga catcgatcgc ttaccagtcg gcataacagc
     9061 cttgtctgct tatctactgc ttattcagcc actaaatact cagatagata ctgtctggac
     9121 aggtgctgaa gggatactag gagcagttac aactgggctc atcatcggat ggggctatgc
     9181 acgtttagta agacttgcga aagggtggaa gcaaagagag aagatacctg aaaatgtttg
     9241 gcagtctttt acttcattga ttccaatcat tgttatagtc acttttacgc ttatcggttc
     9301 agcggttttt caacagttgt tcagaatgac tgttgttcaa ggagtatgga catatgtcca
     9361 gcttcccatt caatcgttca tagacacgtt gtttggtgtg cttttattag gtttttttgt
     9421 tccgttttta tggttatttg gtcttcatgg atctgcgatg gtcaatgggt tggttagtcc
     9481 cgtcttgcaa gcgaattcat tagcgaatgc agaaatctta gcctcaggac aagagttgac
     9541 ggtggcaaat ggcggacata tcgttactca gcaatttctc gatcagttca tgacggttac
     9601 gggtgctgga ttgacgttag gggctgtatt ttttatgacg gtttttgcga aatcgaagaa
     9661 atacagagaa ttaggaaatc ttgctctttt accagccttt ttcaacatca atgaatcaat
     9721 tattttttcc acaccgatcg tgatgaaccc gatgatggct gttcctttta ttttcgcacc
     9781 ggtcgtttca ggattgatta cctattccgc tttatatttt ggattcgttc ctttatttac
     9841 agcagtccaa gtcccttgga caacaccgcc gatcctctcg ggtttcttag taggcggagt
     9901 gcctgcagcg attttacaag gtatcgtgtt gtcaattagt tttttcattt atttcccatt
     9961 tttgaaaaaa atagattctg caaactgcaa aaaggaaaga cagacaaaaa atgatggagc
    10021 agcagaaaag atgaacgact aaaaaacaaa aatactcctg tcgctttaga ttgggaaaat
    10081 caccaatcta ggatgacagg agtatttata gtcatgttta tactagccct ttttggacag
    10141 ctaattgaac aagaacaact aatgtgttca ttcctacgtg tgtgatcatc gatgtccaga
    10201 tccgaccagt tgctttgtac tctaggctga agaagaaacc taggaaaaag tagattagca
    10261 gatgaccatc attatgagca aacgcaaaca gcaaagagct gatcactgct ccgacccaaa
    10321 aatttgaata tcgactgatg atccccagta tactgcggcg gaatacgaac tcttccatga
    10381 tgggtccagc aacagttgtc gccaaaataa aagcgggtgc ttccatgatc atctggatga
    10441 tattttgtgt attctcggaa ggggttgctt tgccaaacaa tacagattct aacatgacag
    10501 cgatgctttg cagaatcaat gcgacaaaga ttccaaccaa tccataaata acaatctttg
    10561 gagcagaagc tttttttacc gctgattcga tcgctaaggg ttcacgttgc ttaaaataaa
    10621 aaatagctag gacaacggcg cctagtatat acatgactgc agttgctgtg atcaattcag
    10681 acgtagattt tacaagtcct atcgaagcta ggatcaaggg tgagataaaa attagaccat
    10741 agcaaaaaat gctgaggaag ctatattttt ttaaagacat gtaattcctc cttttaaggg
    10801 ataacaagca tgcaaggcaa gcttatatcg attgatttca attatatcat aatttcaata
    10861 agcagctgtc agaataaaat ttcttttctt tgatttaaaa gcttgcaaaa aagagggaga
    10921 atgagtatta ttaaaaatgt aggttagcac tcgaaggtta agagtgctaa cagattaaaa
    10981 aaagagttat cggagggatt tatcgtgtta aaaccattag gtgatcgcgt cattatcgaa
    11041 gtcgcaaaag aagaagaaaa aactgtcgga ggaatcgttt tagcttctgc tgctaaagag
    11101 aaaccccaaa ctgggacagt tgttgcagtc ggtgaaggcc gtttgttaga aaacggtgaa
    11161 aaagttcctg ccgctgtaaa agcaggcgac caagttatgt tcgaaaaata cgccggtaca
    11221 gaagtaaaat acgaaggaaa agaatatctg atcgtcgcag gaaaagatat catggcgatt
    11281 gttgagtaaa tttggaagat aaaaaaataa tcaaaaataa tcaatgaggt gaatcaatca
    11341 tggcaaaaga attgaaattt gcagaagacg cacgcgcagc tatgttacgc ggggtagata
    11401 aattagcaga tacagtgaaa gtaacattag gacctaaagg ccgaaatgtc gtactagaaa
    11461 aatcttacgg ctctccattg atcacaaatg atggtgtgac gatcgcaaaa gaaatcgaat
    11521 tagaagatca ttttgaaaac atgggtgcaa aacttgtatc agaagtagca tcaaaaacaa
    11581 atgatattgc aggagacggt acaacgacag caactgttct gacacaagca atcgttcgcg
    11641 aaggtttaaa aaacgtaact gcaggtgcta atcctctagg cattcgtcgc gggatcgaat
    11701 tggcaacaaa agcagcagtc gaagaattgc ataatatctc aactgttgtt gattcaaaag
    11761 aagccattgc acaagtagca gctgtttctt cgggttctga taaagttgga catttgattg
    11821 ctgatgcaat ggaaaaagtt ggaaacgacg gtgtcatcac aatcgaagaa tcaaaaggga
    11881 tcgaaacaga actagatgtt gtagaaggta tgcagtttga tcgtggttac ttatcacaat
    11941 atatggtaac agataacgac aaaatggaag ctgttctaga aaacccatat atcttgatca
    12001 cagacaaaaa aatctctaac atccaagata ttctgccatt gttagaacaa atcttgcaac
    12061 aatcacgtcc attgttgatt attgctgacg atgtagatgg tgaagcattg ccaactcttg
    12121 tattgaacaa aatccgtgga acattcaacg tagtagctgt aaaagctcct ggtttcggcg
    12181 atcgtcgtaa agcaatgctt gaagatatcg ctattttgac aggtggtaca gtaattactg
    12241 acgatctagg tttagaattg aaagatgtaa caatcgaaaa cttaggaaat gcttctaaag
    12301 ttgttgtaga taaagacaat acaacgatcg tagaaggttc tggagaaaaa gaagcaatcg
    12361 aagctcgtgt gcaattgatc aaaaaccaaa ttgctgaaac aacttctgat tttgaccgcg
    12421 aaaaattaca agaacgctta gctaaattag caggtggtgt agctgttgtt aaagttggtg
    12481 cagctactga aacagaattg aaagaattaa aattacgcat tgaagatgca ttgaatgcta
    12541 cacgtgccgc agtagaagaa ggaatggtct caggtggtgg tacagctcta gtgaacgtta
    12601 tcagcaaagt ttctgctgta gaagcagaag gagacgtggc aacaggtatc aagatcgttg
    12661 ttcgtgcttt agaagaacca attcgtcaaa tcgctgaaaa cgctggttat gaaggatcag
    12721 tgattgttga taaattgaag aacgtggaac taggcactgg tttcaatgct gctactggag
    12781 aatgggtaaa catggttgaa gcaggtatcg tagacccaac taaagtaaca cgttctgcct
    12841 tgcaaaatgc cgcttccgtt tctgccttgt tattaacgac agaagcagtc gttgcagata
    12901 aaccagaacc agcagcgcca gcagctcctg caatggatcc atcaatgatg ggcggtatga
    12961 tgtaagaaaa agcaacttgc ttatctacaa ctgatcatta gtgaaagaca gataaaaaga
    13021 ggctggaaca gaagcgttta actccaagaa ataagaagaa attcacggaa attgcttttc
    13081 aaatttttgt gaatttcagc ttatttccga aggagttgct tctgcttccg ctgtttatac
    13141 gtgtttagag cgtgagacaa aagtgttctt tacttttgtc ccacgctctt ttttgtgtgt
    13201 aattataatg aaaatagtta tttttaatag ttataatatt ttatctatta aaagtaaata
    13261 tccatt
//
LOCUS       DADVXU010000076        12732 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.080, whole genome shotgun sequence.
ACCESSION   DADVXU010000076 DADVXU010000000
VERSION     DADVXU010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.080"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="KB953_002061"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="KB953_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBB6867028.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="KB953_002062"
     CDS             complement(322..891)
                     /locus_tag="KB953_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HBB6867029.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="KB953_002063"
     CDS             1077..1979
                     /locus_tag="KB953_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBB6867030.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="KB953_002064"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="KB953_002064"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBB6867031.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="KB953_002065"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="KB953_002065"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBB6867032.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="KB953_002066"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="KB953_002066"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBB6867033.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="KB953_002067"
     CDS             8547..8732
                     /locus_tag="KB953_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HBB6867034.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="KB953_002068"
     CDS             complement(8794..9990)
                     /locus_tag="KB953_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBB6867035.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="KB953_002069"
     CDS             complement(10160..11596)
                     /locus_tag="KB953_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBB6867036.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="KB953_002070"
     CDS             11889..12698
                     /locus_tag="KB953_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBB6867037.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3801 a         2672 c         2250 g         4009 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt ga
//
LOCUS       DADVXU010000077        12699 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.081, whole genome shotgun sequence.
ACCESSION   DADVXU010000077 DADVXU010000000
VERSION     DADVXU010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.081"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..799
                     /locus_tag="KB953_002071"
     CDS             <1..799
                     /locus_tag="KB953_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867038.1"
                     /translation="LFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNH
                     TPVKSLQFVWRLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSN
                     SIHANKDSLTLIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTH
                     FMSNKLTIELDNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGIN
                     KVVITYQYTKLAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(944..1684)
                     /gene="truA"
                     /locus_tag="KB953_002072"
     CDS             complement(944..1684)
                     /gene="truA"
                     /locus_tag="KB953_002072"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBB6867039.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(1875..2510)
                     /locus_tag="KB953_002073"
     CDS             complement(1875..2510)
                     /locus_tag="KB953_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HBB6867040.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(2657..3898)
                     /gene="sstT"
                     /locus_tag="KB953_002074"
     CDS             complement(2657..3898)
                     /gene="sstT"
                     /locus_tag="KB953_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HBB6867041.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            4096..5094
                     /locus_tag="KB953_002075"
     CDS             4096..5094
                     /locus_tag="KB953_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867042.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            5107..7125
                     /locus_tag="KB953_002076"
     CDS             5107..7125
                     /locus_tag="KB953_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HBB6867043.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(7185..7901)
                     /locus_tag="KB953_002077"
     CDS             complement(7185..7901)
                     /locus_tag="KB953_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HBB6867044.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(8128..8670)
                     /locus_tag="KB953_002078"
     CDS             complement(8128..8670)
                     /locus_tag="KB953_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBB6867045.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(8675..8902)
                     /locus_tag="KB953_002079"
     CDS             complement(8675..8902)
                     /locus_tag="KB953_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBB6867046.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(8981..10861)
                     /locus_tag="KB953_002080"
     CDS             complement(8981..10861)
                     /locus_tag="KB953_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBB6867047.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(11050..11664)
                     /locus_tag="KB953_002081"
     CDS             complement(11050..11664)
                     /locus_tag="KB953_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB6867048.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            11806..12366
                     /locus_tag="KB953_002082"
     CDS             11806..12366
                     /locus_tag="KB953_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBB6867049.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
BASE COUNT         3988 a         2499 c         2012 g         4200 t
ORIGIN      
        1 cttatttctc ggattgattg gtgcttttat ttttataaaa aaattaactg ctgatgattt
       61 atttattttc tttcttggtg tatttgttct tttttgttca accaatttat ttccttggca
      121 actttttaac catacaccag ttaagagtct acaatttgtc tggcggttaa atggtttttc
      181 ttctttattt atagcctata cgatgagtat agtgatctat tatatttttt ctactaaaaa
      241 taattcatgg aaaataatgg tctttacgtt tcttgcacta atattgcatc tctcaggagt
      301 aagcaattct atccatgcta acaaagattc attaacactt atagcacctg aagacgctgt
      361 tgctatagct gaaaattaca atcatactga ttatgcaaac aaagagtcaa tctatcatcc
      421 tgatatgatt aataatgatc aatatttact aggtaaccag gaaataaatc caacaactca
      481 ttttatgagt aacaaactta caattgaatt agataattcg aataataaaa atactgtctt
      541 gacgacacct atttatcgtt ataaagggca agtggcttct attaatggca aactagtaca
      601 aacaaaatta tctaagtttg gcacaactga attaactatt cctccaggta taaataaagt
      661 cgttattact tatcagtaca caaaactagc aattgcttca agatatttat ctatcgttac
      721 tttgatttta tttttacttt atcgatttac tttttctaaa caaaaacaac cgagaagaga
      781 agtacaacat tctcactaga tttttattta agaaagactt tttagctgta cacataaaca
      841 ggctaaaaag tcttttttta tatttcttta atttgcttca tacattattt aacatcgata
      901 tttatactgt gaaacatgcg tttcatccat tatcaaagta tttttatcga taataaactt
      961 catccaaaaa cagaccttgt gcagggacgg tttctcctgc atgttgccgc actttgcttt
     1021 caaatatttc atcgattgtt tctatcggca tggcacctgt gccgatttct agtaatgtcc
     1081 ccatgataat ccgcaccatt ttatataaga atccgtctcc gacaaaagta aaatgaagca
     1141 tttctccctt tcgttcgata gataattcat caatcgtacg cactgtggat ttttttgatt
     1201 ttttcaatgc agaaaaacca atgaaatcat gtgtacccac taatttttcg caagctgcat
     1261 tcagcttgtc catatccaac tcttgaggga aataaaagct gtggtttcgc tcaaatgctg
     1321 aaggaatcac gttgttccaa acataatagc tgtatttttt gcccaccgca ttatatcttg
     1381 aatggaagcg ctcaggcatt tcttcgattc ttttgatgac aatatcccgc ggaaggtaac
     1441 gattcataaa gtcgatcatt tctgccagtc caatttctgt gtttgtttta aaatttgcta
     1501 cttgtcctcg cgcatgggtg ccagcatctg ttcttcctga tccaataatt tctatctctt
     1561 catttgtcat ctgatgcaat actttttcga tttttccttg aatggtttta tcgtcatctc
     1621 caagtctttg ccatcctgaa tatctttttc cgtcatattc gattgtcaac ttgatgtttc
     1681 tcatatttta gtggctcctc tgtcttccat ttggtcttct ctctaccttt ttaagattgt
     1741 agcacaaaat atagctcctt gcttctctaa gacagaagct atacaagaaa actgtggaaa
     1801 tatatatttc agaaaaacaa aaaacagcca ccggatgaag tatccgctga ctgttcaatt
     1861 tgacggctca agttttactg tttgagatgc aagatactca accaggcaat gggcacataa
     1921 ttaaagaaaa aactgattct ttggcaaaaa cctattttat tcagtaatgg ccaagaattt
     1981 atagaaggaa ttcttgccag tccataaatc gccgcaaaaa gcaaactgat cacagacagt
     2041 ccaaggtata agtggcttac gtccatttgc ccattttttc gatagatcaa gaaaagaaga
     2101 actggaaaca ataaaaaacc actgtaacct aaacctgatc cgatattatg gacccatgtc
     2161 gagacattcc acgaagattc atgggtattg atactgaaca aacctgtaaa gatacaatca
     2221 cctataccat agaacgcgat ggctgctgta actagggtag ctaacacacg agatgtacta
     2281 acaaatgcag catacaatgc aggcaatgac aggatgaaaa agatccctga aaccacagac
     2341 caaataagaa acgcttttct tacaggactg gaaacctctc caaatacact gatcacgctc
     2401 ttcatttgat ctaattcagg ataatacaat cctaatacgt acggagtcaa aaaatcactc
     2461 agcacgccta ataataaaaa gaaaaaacca aatttacgta aaaaatccat gatacgcctc
     2521 caataacaat tacctatgct tttaattgtt attataaacg actttatctt gatttaacag
     2581 aaacctctat ttacacgaac atctatataa ctcattaaaa actcgaatca ttttaaaaat
     2641 actaaatatc taacgttcaa cgttcactta atccatctaa acaatctttc aatttgattt
     2701 tttccccact tcttttacgc gcgcctagct ctcctattgc agtaaacaac agatcacttg
     2761 acgaatttaa ggcagtttcc attgaatcct ggataacacc gataataaat ccaataccga
     2821 cgatttgcat cgcgacatca ttagagatac caaacaaact acatgctaat ggaatcaata
     2881 acagtgaacc gcccgcaaca cctgaagctc cacaagcagc taaagcagac aaaatacata
     2941 aaaggacttt caaaacaaaa ggtgcttcaa tccctaaaga atgtacagca gtcaacgtca
     3001 tcaaggaaat ggtaatcgcg gcaccgccca tattgatcgt agcacctaac gggatcgata
     3061 tggaataaga ctcttttgtc aatttcaatc tttcagccaa catcatgttt atcgggatat
     3121 ttgcggcaga acttcgggta aagaaagcgg gaagtgcgct ttctttcaaa caaaagaaaa
     3181 tcaatggata gggattttct ttaataaaca aaaacgtgag gaatgggtac acaactaatg
     3241 cgacaaacag catcgtaccg atcaaaacaa gtaaaagctg cagatactct gccaatccag
     3301 cgattccagt cgtagcaatt gattgataaa ccaatcctaa aatccctatc ggagcaaatg
     3361 caataatcac ttgtacgact tgtgacaacg ctatagatat ctgatccacc aactcttttg
     3421 tcgattctga acgtgctcga aaagctagtc ccaacaatac agcccagaat aagacgccaa
     3481 ggtaattcgc atcaataaga gattgaacag gattttgtgt gacattcgtc aacatagtag
     3541 ataaaacact ttccaaactt tgcggtgctt tttcagatac tgtttcttgt aaagtaatag
     3601 gaacagtaaa aattgaactt acaataactg ctacaagtgc agaaagcaat gttcccacta
     3661 aatataagat caaaataggc ttcacgaatg tcttattccc aattttatgc ttggcaattg
     3721 acgccatcgt caagaaaaaa accagaatag gtgcgattgc cttcaagcaa ctgacaaaca
     3781 acgttcccaa tattgaaata aatgtccatg tggggaaaaa gataccaaac aatattccga
     3841 ctaaaatacc aacaacaata cgttgaacta acgacatatc caaaaacttc tctaacaaat
     3901 gaccaccctt tcacaatttt aacaatcatt gtatcatttt atgcaaagaa actaaaggta
     3961 taattttcta aaaattctaa caatacaaac aaccatttct tgctaatttt gtactttttc
     4021 tgtctacacg ttgttttttg agatattcta agttaggatg ttattaatgg gcaacttatc
     4081 aggaaacgtt ttcttatgtt tttgtccaat ttttttgtga ggtgtttatt cttgaaaaaa
     4141 ataaagaagg agagttatgg cttcctgaca atctttgtcg cttgtataat gatggtttta
     4201 cagcgtatat tgatttcagc agtcactatt cctacatatg caacaaaccc tggtcgattc
     4261 tacatctata catttttaca aacttccgtt gttattggtg caaatttgat cccactttac
     4321 atgggttacc aatatcaaaa aatcgcccgt ttaaaagttt tacattattt aagtcgtttt
     4381 gcctttatat tcctaatagc cacactagtt tgcaatatct ttttttacgt acaagctggc
     4441 agcttgaata ttcgtgatta ttggcttatc ctaacaccta tcagtcagaa ttactttact
     4501 tatgctgttt cttgtacttt gcttttttgt agtattcctt atacagttgg atttttaaat
     4561 aaattatcga aaacaacaat taaatatcta ctattatttt taacaattgg attagttggc
     4621 tgctctactc tatttaataa agacccgttt gcattacaga atggacagag tattttatgg
     4681 attttttatt tatttttaat gggttatggt ttgaaagaat ggaactggaa agaaaaggtc
     4741 cgctataagc ttcctcaagt atttatcagc ttgattgttt tattcggttt gattattttg
     4801 atgacacaaa tttcactcat catcagagga aatacagata cagcgctgag gttttctaaa
     4861 ccatattctc tcttcgcaat gtattatact ttctcttcgt ttttattttt agaaatgcta
     4921 tctgataaat taggtataaa aggaagagga aattttcttg ctgtattttt gattgtcact
     4981 caagttatat gtaatttccc tttagtttct tatcgcgtat ctacatttat gaaacgtgaa
     5041 tttccaaact cagggaaggc atggctgata aatatcgggc agttttttat ttgatattta
     5101 ttagcagtgt ccctcctagt tatcttttta ttttatatac aaaaaatccg tttcattcat
     5161 cgtttcatcg aatacttcgc ttttgattcg attgaacaat taactcagcg ttggaaaaaa
     5221 agaattcatt ggctttttgt tcataaaagt tattttttag ttgcagcatt tttttactgc
     5281 tttaccttcg tgcagatttt cgttttagaa ccaaaagaag gttggaaaga aactatccag
     5341 gttgccttaa aaatatttac tcagcgacaa gcacccatga tcctgacaat catcattatt
     5401 atgggatttt tcttcttact attgttgatt tccaatcgtt tttggtacgc cttgactgct
     5461 accttgatca ttaacttact gctcactatt tcgacagtaa tcaaaatgga aatgcgggaa
     5521 gagcctgtct ttccttcaga tttaaaaatg ctgaccggat tgtcagaact attatcgatg
     5581 gttagcccag tcttattaat cgtgggcgga ttgatccttc tcctattatt gattactagt
     5641 attattgtac aacgtagact acaaaaacag tattccttga agattcattg gaaacgtcga
     5701 tttatcagta tcgctgtatt attaggatgt ttttcgggtg tctttttcat taatcacaaa
     5761 aattcgcctt ctttcttgct gtttaattta tttaaagtaa acaagacttt ctttaaccag
     5821 caaggcgccg taaaagataa cggaccagtc attcaattcc taaacaatat cgacattaaa
     5881 atcatggaag aacctgctgg ctattcgaaa gaaaaaatta atggaataat gaaaaagtac
     5941 aacacaaagg cagatgaaat caaccaaaca agaagtgact ggttaaacaa tcagacgatc
     6001 gttctcaatt taagtgaaag cttcagtgat cctgcacgtg tccctaatct gacagtacct
     6061 acagacccta ttccttacat caaaagcatt ataacagaag gaacaggagg attgatgtta
     6121 tccgtcggtt atggtggcgg aacagctaac atggaatggg aaggactgac aggcttgagt
     6181 atcagtaacc tgtctgcttc gttagttaca ccttataccc agctagtgga gcgtcagaaa
     6241 ataagtccaa atattaccga tctattcgat gaaaaaatag ccatccatcc ttttacagct
     6301 gctctttatc gcagaaaaca agtgtttgat aaatttggtt ttgacaggtt ctattattta
     6361 gacagtccgg ataaattaac ttatacggat aaaatagaac aaagcccacg catatcggat
     6421 gattcagcat acaaagaaac cttgaaagct ttacaaagca atacggaaac gtcacaattc
     6481 atccaattat ccactatgca aaaccatatg ccttatgatg attactatag tgaattgaac
     6541 tacactgctg aaggaactgc tgtcattcct aatagaagaa aagaattaca aacttatatg
     6601 caaggccttc actataccga tacagcagta aaaaaattca tccaagaaat cgacaaaatc
     6661 gataaaccga ttacctttgt tttttatggt gaccatttac ccgcaatcta tagtgggaat
     6721 gattcgaaaa aatatggatt agaacagcac cagacagatt atttcattta taacaacaag
     6781 tatagtcgtg aacaagcagc tacgttaaat aaagaagttg ttgctcctaa taactttggt
     6841 gctatggctc ttgagcatgc gaatatcaaa gtaactcctt actatgcgtt gttgacagaa
     6901 gtatctaatc atctacctgc attaacaatt gatcctacag aaagcttgtc taatcgtttt
     6961 aatggaaaac aggtctttgt tacagaggat agtcaaatta tccaagaaaa aaatttaact
     7021 gagaaacaaa aagaaattct agaagattat cgtctgatac aatatgatct tgttgcaggt
     7081 aaccagtaca gtgcaacatg ggcgacacaa aaaataaaag actaacaaat aaactgagca
     7141 aggggttgtg ataaaagaca tgtcacaacc ccttgtttat ctcattaatt tgcttgtcca
     7201 taaacgattt gattaaagcc atttttcaat tcattattcg ctgtctcgtt gaagtaaaga
     7261 taattatcgg gattcccctg attttgttgt gtcaccaata cttttggctg attttgctga
     7321 aacccgaata gatagacgtg ctttttcaga tatggttctg tctctgcatc ggaatagacg
     7381 gctgtgattt gatagtctgc ttgacctgtc ccactttctg accattctgc agaaacaggc
     7441 actccaccaa tagccatctt ccactccccg ttcaaaatag cttgagggac tttaagacca
     7501 tataagtcca cactcatttg attcgtataa cttttgtatt gttgtcccat tgtttggccc
     7561 catttggtca taaatgcctc taattgtgcg gatttgtctg cattccacag tgtttgttca
     7621 acttgttcgc tgctttctga agaggaagat gaagcctcta cagaagaact tgaagtagat
     7681 gtttgagttg tttcagtcgt tttggaagac tctgacgaag tagaggtatc tgctgtttct
     7741 gtacttgctg cagttgactt gctctctgtg ctagttgttg tagtagttgc aactgttgct
     7801 ttcgctgttt cttttgttga ttcttttttc gattcattcc cgttggtaca accagctaac
     7861 aaaaaagcta gaccaattgt tactaaaatc ctttttttca attaaaaaac tcctttcgat
     7921 tactcccact tctttattct atcaaataag caatgtgatt ttattgattt ttcgtatttt
     7981 ctcatcaatc tttcaaatct tctaaaaact gagaagcacc cgctgatgtt gtctgtaata
     8041 catcagcggg tgcttctttt attttaatct tcgtgatttt ccaatttacc tcacgcttta
     8101 tatttgtatt tgaagaaaca tagactttta gtcatcatcg tcttcctcat ccaaatcttg
     8161 tcggacagtt tttccttgga gtctttgtac ttggtagatg atttctttat tgaaacttaa
     8221 aagttcaaca agcaattgtt gcaagccata tttttcttct ttttcagcta atttgattgc
     8281 ccgtgtgata aagacatttt ggaaatcaaa atcttttgca aagttttcca acattgttga
     8341 ggctggtaaa tatttgtttt gaccatgttc gtaaattttt ccataacgca tgacttcttc
     8401 taaagtagac ggaacgattt cattttcaat cactaactca tgagagattt tattgaacca
     8461 tctcgcttct tgatcagcat attctgaaaa aagggtactt aattcttgcg aagatgtacc
     8521 caaaacatac aattgtgctt ggatcaattt catttcttct aatttaaaat ttgctaatac
     8581 atgccccacc atggcgccag cagttggttt atgatggtcg atatctgctt gttttgtttc
     8641 ttgaacaaat ttttcttcca ctatattcat tgatttacgc ctcttttact tttgctgatt
     8701 ggacttcata acctaatttt tccacaactg cagctaattg ttcagggtgg acttcatctg
     8761 gatcaaagtt tgctttgatt ttgctcgcat taaacaaaac tttcgtattg cttaccccag
     8821 gctgttgaga aaccgcatgt tcgatttttt gcatacagct tggacaagct agtgtatcta
     8881 attgcataat aatttttttc atcgtagttc ttccttcctt tttcttgata gtcttattct
     8941 agattacagc ttccataaaa aaattgattc ttatcaagtt ttacgatttt tcttctttgc
     9001 tttcgtgtcc aaaatacaat aaacgcatcg cgttaaagat aacgatcaag atactagctt
     9061 cgtgaacgaa catcccacta gccatgtaaa taaacccggc aaataagccg ttgagtaaaa
     9121 ataagacgac accaatggca atcactacat tttcacgggt atttaaaaca gttttcttag
     9181 ccaagcggta ggcatgaacc aacgattcaa aattagattg catcaagaca acgtcactcg
     9241 tttcgatagc aacatccgtt cctgatccca tagcaatccc gatatcagcc gcagccaaag
     9301 agggagaatc attgattcca tctccaataa aagcaacgcg caatccttgt tcttggtatt
     9361 ttttcacaaa ttgaaccttt tcgtctggta ataattcagc atggacttca tcaatgccta
     9421 acatatcagc tacataatca gcagtcatct gattatcacc tgtcagcatc actaactgtt
     9481 ttgctcctgc ttgtttcaac ttagctaatt gttcagcgac ttctggtcga atcacatctg
     9541 acacacctat gatttgagtg atttttttgt cgattgctac aataatgacc gagctgcctg
     9601 tttgttgtaa ttgatgtaaa tcttgtactt gctttggtgt gagagaaagt ttgtgagcgg
     9661 caatcaattt ggtgttgccc gcacaaactg tatgaccatc aatctcagct gtgattcctt
     9721 gtcccttgat tgtctgatta tctttgactg ctaattgttg aaaatcaatg tctttctcct
     9781 gagcataatg gatcacggcg cgccccagag gatgatcgga taacgtttct aatcgagcca
     9841 ctaaagctaa gctgtcccct tcatcctctg tatagttctt gatagtggat acagcagtag
     9901 ttcctttggt caatgtacca gttttatcaa agacaaaagt atcgattttg gaaaaagtat
     9961 ccattgcttc accgcctttg actaagatgc cccgtttcgc tccattaccg attcctgcta
    10021 cattcgaaac aggcgcacca atgactaaag cgccaggaca accaagaacc aaaacagtga
    10081 ttgctaaacg aaaatcacga ctaatcagac caacaaccaa cgcaataagt aaaacggttg
    10141 gagtataata ctgagaaaat cgatcaatga atttttccgc atgggatttg gtatcttgtg
    10201 cttcttctac taattcgata attttggcaa aggtcgtatc atctccgacc ttagttgctt
    10261 caatcgtcac gtagccatct gctaaaatcg ttccagagaa gacttcatct ccactgattt
    10321 tgacgacttg tcgtgattca ccagtcactg cggcttcatc cgcataccct ttcccgtcaa
    10381 tgactcgtcc gtccacagga atagctccac cagtttttac taagacgata tcgccttctt
    10441 ccacgtcatc gatttccact tcttcagtcg ttccatcttc ttggacaaca ctagcagtag
    10501 atggggccat ctcggtcaaa gccttgattg actcccgcgt tttggtcaat gttttttgtt
    10561 ccagatagct accaaacaaa aacaagaatg taacgatagc tgactccttg tattctccga
    10621 tcataaaggc accaagcaca gcaatagtaa cgagtaaatc aattgagata actttgactt
    10681 tcactgcctg atacgcatgg atcataatag gtatgaccgc aataatagaa gctatggcta
    10741 gagcaatgtt atagaacaag acaaaaccag taaattttcc gataaaacca aaaataatca
    10801 acacagctga taaggcggtg atatgatttc gatgctcttg gatccacttt tgtaatttca
    10861 tctttctccc tcttttcctt tcattcttac gagactaagt atagatactt atccatcata
    10921 aaaaattgac cactatcaag tttagtcaaa aaggcggttt tcttttggta gaatacacta
    10981 caaaaaatag ttagcaagaa gactcactaa aagtgatctt gccaactatt gtcgtttcta
    11041 tcgaaatttt cattgaatga ttttcctgta cagcatacat cggagatcta tttctttatc
    11101 taagattttt atcttgctaa ttttttcctt tagtctttca aatccacgtc gttcatagaa
    11161 ttgatacgta cagccactat ctgtaaaaag gaatatttct tttcctttct ctcttttttc
    11221 aaattctgtt agcaacttgg taccgatgcc ttttgttttc aaatcaggat ttgcagctaa
    11281 aaaaatgatc tctccatctg gcgaatgctt ctttaaatat gctgcaagca tttgttcatt
    11341 tgctgtctcg taagctcctt tactttcttt agcaaatata ttttgtaaaa aatcgaatat
    11401 cttaacatat atttgctgtc caaaagaacg atgtttcttt ttttctcctt ttatctccgc
    11461 cagcaaaaca cctgccaact cgtcctctgc atacaaagca agaatttgag ttgctcgatt
    11521 caactccata tacaaaaaat atcttccgta aagattcagc agaagttcat tttccatata
    11581 tctctcaaag ttcattcctt caacagcaaa atagatagct tttttgtaat ctttcttttc
    11641 taagggttta aaagttagtt tcatcatttc gcctcctcaa cttcaatgat tgtgttatac
    11701 ttacattata atagacacag tgtctactat ctataatcaa tcatctataa gtttgtcgat
    11761 taagcgacag ctttgctttg atatgtcgga taaggagata ggtaaatgaa aaagacagat
    11821 agacgcgtca aaaaaacaga aaaagctttg gctgaaacac tatccacatt attagtaaat
    11881 aaaaagattc aagcaatcac tatacgcgaa ctaacagaaa cggcagatgt acatcgttcg
    11941 actttttata cacattataa ggatatctat gatctatatg atcaattaga aagcaacttt
    12001 tttcgggatc taaatgaaat tctctcttat gatccagccc atagctatga ggaactatac
    12061 actcgcttga ttgactatgt ttacgcaaat acttttttat atcgcttatt tatcctcaac
    12121 catggtgata tttcgtttca gaagaaagtt aaacaagtta ttgaagaaaa ctatctgaac
    12181 atatggctat atgaagacaa gaaaacgatt atcacagaag aaatgagatt ctttactacg
    12241 taccacgtcc aaggttgtct atccgttctc aatcggtggg tagaaagcaa ttacaagtac
    12301 tccaaaaaag aattattaga actcttacag aaactgaatg atcacataga gaaaatcatg
    12361 ccttgaataa aaacatacta agcttagtta agaagaaccg taaccttctc gtataacaac
    12421 tgaatcttat ttagccaaat tgtttactga agattttttg agtaaaattt ctataattcc
    12481 tacttattga aaagtagttt gtagtgctgc ttctccctct ctaagtgata tatcttgttg
    12541 taccaatcaa attcacctca tcattaataa attactttta ggctaattta ttacaactta
    12601 taggttgaag ctgtaaccaa tttatagtca tattatattt ttatcatcca gtttattgaa
    12661 ttcttctcgt ttacctcaat tcattttcaa ggggaatca
//
LOCUS       DADVXU010000078        12605 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.082, whole genome shotgun sequence.
ACCESSION   DADVXU010000078 DADVXU010000000
VERSION     DADVXU010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.082"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(51..1580)
                     /gene="ahpF"
                     /locus_tag="KB953_002083"
     CDS             complement(51..1580)
                     /gene="ahpF"
                     /locus_tag="KB953_002083"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HBB6867050.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(1590..2153)
                     /gene="ahpC"
                     /locus_tag="KB953_002084"
     CDS             complement(1590..2153)
                     /gene="ahpC"
                     /locus_tag="KB953_002084"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HBB6867051.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(2297..2959)
                     /locus_tag="KB953_002085"
     CDS             complement(2297..2959)
                     /locus_tag="KB953_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBB6867052.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            3143..4789
                     /locus_tag="KB953_002086"
     CDS             3143..4789
                     /locus_tag="KB953_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBB6867053.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            4780..5862
                     /gene="uxuA"
                     /locus_tag="KB953_002087"
     CDS             4780..5862
                     /gene="uxuA"
                     /locus_tag="KB953_002087"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBB6867054.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(5970..7523)
                     /locus_tag="KB953_002088"
                     /pseudo
     CDS             complement(5970..7523)
                     /locus_tag="KB953_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            7671..8812
                     /locus_tag="KB953_002089"
                     /pseudo
     CDS             7671..8812
                     /locus_tag="KB953_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(8938..10017)
                     /locus_tag="KB953_002090"
     CDS             complement(8938..10017)
                     /locus_tag="KB953_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBB6867055.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(10120..10560)
                     /locus_tag="KB953_002091"
     CDS             complement(10120..10560)
                     /locus_tag="KB953_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBB6867056.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(10617..11027)
                     /locus_tag="KB953_002092"
     CDS             complement(10617..11027)
                     /locus_tag="KB953_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HBB6867057.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(11207..11641)
                     /locus_tag="KB953_002093"
     CDS             complement(11207..11641)
                     /locus_tag="KB953_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBB6867058.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(11741..12589)
                     /locus_tag="KB953_002094"
     CDS             complement(11741..12589)
                     /locus_tag="KB953_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBB6867059.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT         4212 a         2347 c         2243 g         3803 t
ORIGIN      
        1 cttgtcacca tccttttttc cgctctctga ctttttacaa tcttcatagc tcaagtgcgc
       61 atcagataat cgaatgctcc taaagctgca gtagctcctg aacccattga aacaatgatt
      121 tgtttgtatg gactatctgt acaatcacca gctgcgaaaa cgccggggat attcgttgcg
      181 ccatttttct cggtcactat ttcatttctt ggattcattt cgatcgttcc atctagccaa
      241 tccgtattag ggagtaagcc gactagaatg aatactcctg aaacagctag tgtatgcatc
      301 tccatcgtac ggcgatcgat ataggagaga gattctacct gattcgtacc gctgatttct
      361 ttcgtcgctg catgtttgat aacagtgaca ttcgcaagag agactagttt atcctgtaag
      421 atttgatctg cttttaattc tggtaagaat tctagaacat atacatgttt tgctaaccca
      481 gccaaatcga ttgctgcttc aacaccagaa ttaccaccac cgataacagc aatctctttt
      541 cctttaaata aagggccgtc acagtgtggg caataagcaa ttcctttatt tttaaactct
      601 ttttcgcctg gtacattgat agaacgccag tgcgcacctg tcgcgatgat ggcggttttt
      661 gctttaagtg atataccgtt agctaagcca atattaataa aatgttgctc tttatttaag
      721 gaaacggcct gttgattcat cataatgtcg atagggtaac tacgcacatg actttctact
      781 tgctgcatca attttggacc ttctgtgtaa gaggtaccaa tcatattttc aatccctagt
      841 gtttcaacga cttgtccgcc gaaagaatca gcgacaagtc ctgtacggat tcctttacgt
      901 gctgcataga tagctgcact actagctgct ggtccaccgc caatcactaa tacatcataa
      961 ggttcttttt caaacaactc ttcttcaatc aaagggccag tgattttttc aacaatttgt
     1021 tcgatcgtca tgcgtccgct actaaattct tctccattta aaaacacagt tggcactgcc
     1081 atgatttttt tatcatcgat ttctttttgg aacatcccac cttcaatcat cgtatgagta
     1141 atatttggat ttaatacagc taatatattc aatgcctgta caacgtcggg gcaattatga
     1201 caagtcaagc taacataact gtcaaaatgc aatgtttgtg ttattttttt gattttaaga
     1261 ctcaaagatt cactgatttt cggtgcccgt ccactaattt gtaacatagc cagtaaaaaa
     1321 gaggaaaatt catgaccgag ggggatacca gcaaaaacga ttccactttc tttgccaact
     1381 ggattgatag aaaaactagg tgtgcgcatc gaatctcttt tttcccaaga gattttagga
     1441 gacatttctg ctatttcctt cgtaaattca gctagttttt gactattttc actattatcc
     1501 aaactaacag aaaaaacaat aggagattca atcagtgcta aatattgttc gagattttgt
     1561 ttagtttctt tgtctaacat atccaccacc taaatctttc caacaagatc aaaacttggt
     1621 ttcaatgttt cgccactttc tttccatttt gccggacaaa cttcacctgg gtgggtacgt
     1681 acatattgag cagcgcgaat cttatcaatc agtgcactag cgtcacgacc gattccatct
     1741 gcattgattt ccatagcttg aacgattccg tctggatcaa taataaatgt tcctcgctga
     1801 gctaatcctt gctcttcatc caaaacatca aaaatacgag aaatctgatg agacggatca
     1861 ccgatcatca cgtattcgat tttaccgata gcatctgatg tatcatgcca tgctttgtga
     1921 gtaaagtggg tatcggtaga gcaagaaaag acttcaacac ctaaagattt caatgtactg
     1981 tattgttctt gtaagtcttc taattcagta ggacaaacaa aagtaaaatc agctggatag
     2041 aagcaaagaa tactccagtt ccccataaga tctttttcac tgacttctaa aaattctcct
     2101 tgatgataag catgtgcatt aaattcttcg atttttttcc caatcaatga catagtaaaa
     2161 cctccaataa taattattta tttataatta ttgtaattta gttggagttg caaagtcaat
     2221 ggatattttg ttatttaata aagattactc aaaaacgaag gttaattata aaaatagaag
     2281 aggtcgcaag aaaatgctac tgctggaaat actcgggata acgttgagta acagtttcct
     2341 gttctttcag catcatgtgt aagtgcaatc ccgtttgaaa tcgagcttct tctggttttt
     2401 tatcaactaa agccttgagt aaatcataat gttgttccat gatcgtttcc catggaagcc
     2461 cctgaatggt cactcgtaac cagcggaaac gttctaaatg ggtattatgg tcatctaacc
     2521 agttccaaac ttcttttctt cctgcaattt cgaaacaagc ttcatgaaat aaattatctg
     2581 cacggaaaaa agcgcgttcg tcatttattt tgactgcttt ttcttgttct tctaaaatcg
     2641 tttctagcac tattcgtgtt tgtgtgtcca atttggaaca acattcaatc atgatttgct
     2701 cttccaactg ttctcggaca aagcgagcat tttctgcaga aacaagatta attttggaaa
     2761 catacgtacc actttgaggt atcgtataca cgagttcttg ctgacgtagt tgaatcaatg
     2821 cttcgcgaat gggagtccgg ccgatagcaa gatgttcctc cagtgccttt tcagatattt
     2881 tccttccagg ttctaagtca gcatagataa tttgtttacg aatcgcttga taagcttgtg
     2941 attgcaagct gcttttcatc tttattcctc caaaatcatt tctatgaacg tagtataaca
     3001 gaagaggtct gaaaaacaat ctggtatgtc acattaacta gtaaatttgt ataaatagta
     3061 aaattttggt tgacatagcg atttttattg cataaaattc acttgaatac aaaaactaat
     3121 atgttagttt aggaggggaa aaatgattga tttaaacaac tggaggaact ttatccctga
     3181 attatctcaa aaagaagtag aaatacctca ttttgaccgt gcatcattaa gagaaaatgg
     3241 acaaaaaaat cctgtctgga tccatttcgg tggcggaaat ctttatcgag gatttcatgc
     3301 agaaatcgct caacgtttag cggatctagg cgaattgtcc actggtgtca ttgtttgtga
     3361 aacattcgat gaagaaataa ttgaaagggc ttaccaagat tttgatgatt tgattcttga
     3421 agtggtcatg ccaagcagtg gagagttcgt caaacgtata ttagctgcga cagctgacag
     3481 tctgtattgt catccagatt gttctgaaca ctttgataag atgattcgac actttgaaaa
     3541 tcctagttta caattagtta cttttgctat tactgaaaaa ggttatcgat tgaaagatac
     3601 aaataataaa tatataggac cgattgcagt cgatataaaa aatggtccaa agaagccatc
     3661 acatacaatg agtattcttg ctgtgctact ttataaacgt tttttatctg gagcttggcc
     3721 gattgcactt gtctctacag ataatttttc acaaaacggt agtcaattac gtacttcttt
     3781 gctaacgatc gcaaaagaat ggcaaaagga aggttatgta caaaatacat ttatccaata
     3841 cttatcgaat gaagaaattg ttgcgtttcc atggacgatg attgatcgta tcaccccgaa
     3901 tccttccttg tcagttagca aaatgttggc aaaagagggc ttttcaaata tgtcgatttt
     3961 ccaaaccgca aaaggaaata atattgctcc atttgtcaat acagaggaca tccattattt
     4021 aatcatggaa gatcattttc ccaatggacg tccgaaattc gaaaaagctg gggtattctt
     4081 gactgatcgt gcaacggtag ataaagcaga tacgatgaag gtgacgactt gccttaatcc
     4141 tttgcatacg tctttagcta ttatgggttg tttactgggg tataggagca tagctgagga
     4201 gatgaaagat ccagatttat cgctgctagt ttctgaaatc gggtacaatg aaggcctccc
     4261 agttgtggag tctccaggta tcattgatcc aaagatcttt ctggaagaag taattactgt
     4321 gcgcttttct aatccaaata ttcctaatat gcctcagcga atcgccacgg atacctctca
     4381 aaaggtggcg attcgttatg gtgagacgat taaaaaatat atggaaatag aagttaaaga
     4441 cccgcagaca cttgtattca taccattgac tctcgctgct tggcttcgat accttattgg
     4501 cattgatgat caaggaaaac cattcatccc gagtccagac ccactattga cagaccttca
     4561 gcataagctt tccgatattc aactgggtac gcaagataca tcacatattc atgagaaaat
     4621 ggctcctatt ttgtcaaata aagaaatttt tggtgtagat ctttaccaag cagagcttgg
     4681 cagaaaaata gaagaaatgt tcatcaagat ggtagcagga ccaaatgccg tacgaaaaac
     4741 attacaggat tacttacaag aacatggagg aaatttatca tggaaatgac ttttagatgg
     4801 tacggatcac aggaagaaaa gattacctta gatgaaatca gacagattcc tgcggtcaaa
     4861 gggattgtag gcacattgat ggatatacca gtaggagagg tatggcctag ggagaggatc
     4921 aaagaactaa aagaagaaat tgagtctcgt gggttaacac taaaagtaat tgaaagcgtc
     4981 aatgtgcatg atgccattaa aattggatta ccttcccgtg agcagtatat cgaaaactat
     5041 aagcagacaa tccgaaactt ggcagaatat ggaatagaag tcatctgcta taattttatg
     5101 cctgtatttg actgggtgaa atctgacctt gactaccgct tagaagacgg ctcatccacg
     5161 ttagctttta tatctgatga tattccagat gatccaagag aaatcgtcgc gcgcatcgaa
     5221 cattccagca atgaatttgg attgccggga tgggaacctg agcgcttagc tcctatcaaa
     5281 tccttgcttg aagcctatgc atcagtagac gaagaaaaac ttcgagaaaa ttttgcctat
     5341 tttctccaat caatcatccc aacatgtgaa gaggtagggg tcaaaatggc tgtacatcca
     5401 gatgatccgc catactcgat ttttggatta gctcgaatcg taaagaatcg agaagattta
     5461 gattggttgt gtcgcatagt agacagccct tataacggaa tcactctttg tacagggagt
     5521 attgcagaag atccagaaaa tgatgtatat gggattttag cagaattttg ccaaagagat
     5581 cgaatcccat ttgcacatgt tcgaaatatt aaatttttag ctaatcgaga tttctatgag
     5641 gcaccacata aatccgaata cggttcatta gacatgtacc gcatactaaa aacaatgtat
     5701 gacaacggat ttaacggata catccgtcct gatcatggaa gaatgatctg gaatgagaca
     5761 ggaagaccag gatacggctt atatgatcga gcattaggtg ctgtttatct aaatggatta
     5821 tgggaagcaa tcgaaaaagg aaacaaaagt aaagataatt agtgaaaaaa actgtaattt
     5881 tattatgtaa ataagaagga tgttcaaatt cttgatacat aaatccggag gttgttaata
     5941 agcattcgga tttatgtatc ttatttggtc taggaagtaa cttgattttc ttttttcgat
     6001 tgatccatga gtgcttcttc ttttaattca aagatatatt ctgagatcct cataatcact
     6061 tttttccagt cttctggtgt gatgattcct tttaaaatat agattttttt gatatgagaa
     6121 ggcaattgtt tcaaagggca gtcgctgata acgatgttgc agttctccat aaaatcattt
     6181 tgtattttga tcgattcatt tgaaaaaatt tgttctatct taacgatcag atctttactg
     6241 atataaaaat cacgcgtata gttcacacca atcacaattc gcggctgttt tttgtagcga
     6301 tcatacaaat gtactaggtc ttcaaccatc cattgcacat tttccttttc taaaataact
     6361 ggtaacaagt gttgattttg ttctctgaaa tccatataaa attgttcaat ggcggtttgg
     6421 acttctttca atacgctatg ttcttgataa agcatgaaag taggaagggg cgtatttggt
     6481 tgattgaagt taagaaatcc attataaagt ttatttcgtg cgaatgccaa tgagtaaaga
     6541 atcttttctt tttcaggtat aaacaaatca aatacagtac aaagggactc caccatttct
     6601 cttgaataaa gaacatgagg aacgtttgct ttttttaaaa gtttgtattg ctccaagctt
     6661 gtttcttccg attcgatttt cgtgatagaa agtcgagcta aaatattcac taagatgcgt
     6721 tgttcgcgac aaattggcag attaggtcgc aaaatatcta cagagagatc aataaaaaag
     6781 tcaatatatt catactctgt gaattctttt tgtacgtctt tcacagaagt tgcaggaaaa
     6841 ttctctttac atatttttaa tagaaaatat aatttatcta tcataccgct atttagtcgg
     6901 tcaagtactt cttttttaaa aaaagtattc atcataaaag ccactacttg cggatcttca
     6961 tcataaaatt ctgtagcagt acttgaaaag atgttccaat aaatatctaa tgctgcatat
     7021 tgaatttgta cctcttttcc agaaaaagac tttctgccat tattagaaaa ggagatagac
     7081 aaaccgtact atgctaaaaa tgcattaatc tttttcattt tcgtatacaa ataagaggca
     7141 ctatagtttg tttgctgact gaaactgata atcgaatttg tctgacagct gaacaaagaa
     7201 gtcaagatct gataactgac tgattcgttg cagtatcttc caaatagacg ataatagcag
     7261 taagccggat caattccttc aaaattcaac tttaaaaaag gtttttgctt atcaatatgc
     7321 agttctgtta gattgagacg ctcaaagtct tgttctaatt ctttgatgta tagtaaaaaa
     7381 tttgattcag acatatggaa atgatctgct aaatattgtg tagtaataaa gaaacgatgt
     7441 tcagatagga aaagaaaaat atctaatttt gtattgcttt ttttcttcaa gataaacgtt
     7501 ttaatcgctt cttgagtttg catgaaagag gctccttttt acttaaattg ttaaaacttg
     7561 aaaatattac tgtttttttc ataaatttag acaaaaaaac agtaaacata taggtagaat
     7621 ctctactatc ttatttcttg catacaaaaa aatcaaggag tgagagagtt atgagatatt
     7681 caaccatctt aatatcaaca tttgcagcct taagcttatc gcttactata aatcagacag
     7741 tccacacaga ggagacagaa acagccaaat ggacagaaac caagtgggcc aacgtcaatc
     7801 gaattacctt tcaggatgaa ttttcatcct tcaattcagg agtcgcaaaa ttaagaaaag
     7861 gacaaacagc gaccatcaaa gcaaaaattg actattcggg agacttagca cctatactac
     7921 aatcagctgt tacatttgag aatgttgatc ccactttgtc gattggctat gacgagaaca
     7981 ctttgactat ccaacacaag caagcgatat ttaccttcac cgttacatta aatcaggact
     8041 tgttaaaacc agcgcttttt actgtcaagg tatcagatgg actaccgaac gatcatcatt
     8101 taacgccgta tagtcagcgg cagacaattg agcaagattc agccatcgac tcaggaggag
     8161 atcttgttga agaaccaaca gataaaccag aaaatgaaaa taagccagaa gtaccgccta
     8221 cagaaaatcc agacggagaa cagaaaccgg aaatagaacc tggagaagaa cccgatacag
     8281 aaactcaacc agagccagac aataaatcaa aacctgaaat aacccctgga gaaaaaccag
     8341 atgtaactcc agaaccagat acggattctg gaaaccaaac agtaccggaa acaaatccag
     8401 acacagataa tgaaactgaa aatccagaaa aaccagaggt agatccagaa gaaaaaccag
     8461 atgtgactcc agaaccagat acggacgcaa gagaccaagg aataccagaa aaaataaaca
     8521 aaaaaacaat tcaagaggat ggaaaaaaag agagtaaaaa atcaaatcta gcaattctaa
     8581 aaataaatga agagcagctg aataaaaaat cacgaatatt tgatagcgcc caatcagctg
     8641 aaaccttaaa aagtagcaag gatacaacct ttgctgtcct gaaacgaaaa ataaacagct
     8701 accaaaatcc ggagagtcac aaaacaaagt tatactgtgg agcggaatca ttctattaag
     8761 tatagcaaca atgctttctg caaaaagatt taaacaaaac agatctctct aacatttgaa
     8821 taatgtctgt gaacgcattt ttcaaataaa aagaaatatc cagaaaatga ccccaaaata
     8881 ccagattgaa atctgatatt tggggtcgtt catactttat ctttcttcat atttatttta
     8941 agggatatct gtttcttttg gaagatggtt cgggatcttt ttcactttca atacatctaa
     9001 aaagaaggta aagatcggaa tgccgacaat caagccccaa acgccaaaga accgttcgct
     9061 aacaagtaag accacaaatg tataaaaaat cggcagttct gttttgctgg acatcaactt
     9121 cggattcagc acataagact caattagatg gacaatcaaa atcgtaatca ggatataaac
     9181 aacatctcgg atgcctcctt gggaataagc tatgaaagat agaggaatac aagaaatgat
     9241 aacacctgct actgggatca gacttaaaat gaaaatcatg atcgctaaac tcagcagctg
     9301 atgaaagcca aatacagcca aggcgattgt agtgagcaac gtatttaaaa gagcgacaac
     9361 aaattgagct tctaatacca aaccaaatgt attgacaaac ttatcggcaa aatagtaaat
     9421 atcttgaaag aaccaagaaa attcactttt taaaaacaat cttgagaata caattgtttt
     9481 tttcttttca atcataaaaa agaaacttaa gatgaatgac atagcaaacg ccactgccat
     9541 ttttcctata tcctgtaaat agccaagcgc aatcgaagcg ccattttgaa gctgttcaaa
     9601 aaaatcgttt cgctcaatat aagtatggat atattgtaaa acaggatctg catcttttgg
     9661 ctgatgctcg taaaaattaa taacggactg gatcatgtca aatgtttgat gaataagcat
     9721 cgggatatac tttgtgaatg ccagatagat aaggtaaacg atcagtgtat aaatcatgat
     9781 agctaaaaca cctgtaggga ttcggacaaa tcgctgaatg atttgaacaa gatgaatcat
     9841 caaataagta aagataaatg tgagcaaaat ggttgtaatc atacttctaa ctaagaaaag
     9901 aacaaaaacg acaaataata aaacagtgag acggcgtatt ttttcattcg ccaaaaaacg
     9961 ctgataaacg gtcaaatcga caacctccaa ataacaaaaa ataaacacgt tattcattat
    10021 atgatggaat atgaaaataa gaaagagcaa aaagaaaaac cgctaaatga cgttatctta
    10081 tgagttggtt ttcctcataa aatcgttcat ctagcagttt cagccctttt tctggtcttg
    10141 atgaaaatta tctagcattt ttcgggtcag atgttgcagt gtatccaatt cttcctgttc
    10201 aagtggaaga taagacttga tcttttgtgg aataccttct gcttgttttt gcaacgcagc
    10261 accttgagca gtcagcgtaa catttacgat tctttcatca cccgtttcac gctgacgatt
    10321 aatataattt ttttctgcta acttttttag taagggagtc agcgtaccag aatccagaca
    10381 caaatcattt cctagctctt tcactgaagt ctttttcttt tcccataaaa ctaacatcac
    10441 aagatactgg gtataggtaa gattcagcgg cttgaggagg ggagtgtagt gtctaatgat
    10501 ttctttagag gcggcatata aaggaaaaca tacttgatta actaaatata gatcttccat
    10561 ttttccacct ccgctgtttt actatagtgt atcatagcgg atttacacaa aaaagtctat
    10621 tcactaactg ctttcagagt aacttctata tttccagctg tagcttttga atacggacac
    10681 actttatgcg ctgcctctaa taattcttgc gtttcttcca gagaaagacc ttcgatagat
    10741 ccttcaatct ctacacctaa gacaaagctc gtttcacttt ctgaataaag ggaaacttgt
    10801 gcactgacag tagaagcccc agagatattt ttttgctgtt tgatcaattc taatgcactg
    10861 ttaaaacatg cactgaaacc agctgcaaac aattgttcag ggttggttgc attttcgacg
    10921 cgtttaccag gagctgcaat attcaattcg aatgtacgat cgggattatg cacttctcca
    10981 gagcgtccgc ctgtgttgat cattttcgta gaatacattt ttttcataaa gttaccacct
    11041 ttaattatat tttatacaat tgaattttac acaatataat tgtggaaagc aaagaaatgg
    11101 attggcataa gataaataag agaaagcaca tgaaacagac gcgctttctc gaatagcaaa
    11161 catatacaaa aaatatcaga gacaaaaaat aatctcatca taaacattac atatatttct
    11221 caaaaagttg ttttctttct tcaaaaatta cttcacgatg gacctgatga agcatccata
    11281 aataaccaaa aggatctctg aacattgagt tagtcacacc aaagtccatc atttctgtca
    11341 tttcttgaat ctctacacag ccatttcgta atgctttatt atgtgtgtcc tcgatatcag
    11401 agacgatgat attgaaccag atcgtatttg gatgttcagc agtaggagca gtcaaaccaa
    11461 gagacgtgtt ttcatccagc atatggaatc tcgtaccata caaggtaaaa atgacttcat
    11521 tattgcctct agggtaatct gttgcctcaa tcagttgtat ttcatcaaag attgtttgat
    11581 acagttctag tgctttcaaa ctatcattta atacaaaatc gatttccgtt cctttatgca
    11641 taaagatctc ctctcctttt cagtcgaaca gttactagat tcttttatta taccagctaa
    11701 agaaaacgca ttcaaaaaaa tagcggattt acttaccaac tcattcgatt ggatgataag
    11761 taaatccgcg tcccattact tctgctacgc tgctgacaat gacaaaggca cttggatcga
    11821 tttcttctat caatggtttc attcgggaaa attcatgatc agtcacaacg atcaataaaa
    11881 cctcttttga ctttcgatta tacccgcctt ttgcctctag cagggtaagg ccacgtccaa
    11941 tctcagcact taagcgaagg cggatgtctt ccaaataatg ttcactcatg accataacag
    12001 atttctttcg attgatacct gtttcgatat aagtcatgac aattgaagtg attgcaatag
    12061 ataaaatagc ataaagaaac tcttctattc caaaaataaa cagattaaga gaaacaacga
    12121 atgcgtctgt aaaaaataag ccaatcgaag tattcaagtg gaaatatttt ttaaaaatga
    12181 ggggagggat cgtcgtaccg ccgctagaag agttgttttt atataaaatc gcaactccta
    12241 aagcaaaaat cgcacttcca aaaatgacag acaacaaccg atctgaagta atcattgttt
    12301 ctggaactat tgcaatgatt aaaggaaaaa ccaagcttcc gaaaagtact ttaaaaaagg
    12361 gttttttacc taaaaataga agagctagta ccaacataac gatattcaac actaaaataa
    12421 ctatagctcg gtcaaagcct aaagcagctt ccagtaatat tcctagaccg ctgacacctc
    12481 cagcagcaat atgatgaggc cctaaaaaca tattgatagc gactcctaat atgaagaggg
    12541 caaaaataat cttaatgtat gtccatataa attgtctcgt ttctttcatt tattcacttc
    12601 cttaa
//
LOCUS       DADVXU010000079        12472 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.083, whole genome shotgun sequence.
ACCESSION   DADVXU010000079 DADVXU010000000
VERSION     DADVXU010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.083"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..191)
                     /locus_tag="KB953_002095"
     CDS             complement(<1..191)
                     /locus_tag="KB953_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867060.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGC"
     gene            complement(201..1016)
                     /locus_tag="KB953_002096"
     CDS             complement(201..1016)
                     /locus_tag="KB953_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBB6867061.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(1278..1721)
                     /locus_tag="KB953_002097"
     CDS             complement(1278..1721)
                     /locus_tag="KB953_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HBB6867062.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(1752..1865)
                     /locus_tag="KB953_002098"
     CDS             complement(1752..1865)
                     /locus_tag="KB953_002098"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBB6867063.1"
                     /translation="MEADNSNTLALLLEKLIRDYPSVQFHIHSGTSDDITE"
     gene            complement(1855..2193)
                     /locus_tag="KB953_002099"
     CDS             complement(1855..2193)
                     /locus_tag="KB953_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBB6867064.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(2181..2558)
                     /locus_tag="KB953_002100"
     CDS             complement(2181..2558)
                     /locus_tag="KB953_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBB6867065.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            2782..3975
                     /locus_tag="KB953_002101"
     CDS             2782..3975
                     /locus_tag="KB953_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB6867066.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(4141..4563)
                     /locus_tag="KB953_002102"
     CDS             complement(4141..4563)
                     /locus_tag="KB953_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6867067.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            4958..5152
                     /locus_tag="KB953_002103"
     CDS             4958..5152
                     /locus_tag="KB953_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867068.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            5149..5634
                     /locus_tag="KB953_002104"
     CDS             5149..5634
                     /locus_tag="KB953_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867069.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQEAI
                     AQQQAEQARQQSAAEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATL
                     S"
     gene            complement(5844..7016)
                     /locus_tag="KB953_002105"
     CDS             complement(5844..7016)
                     /locus_tag="KB953_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBB6867070.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(7222..8037)
                     /locus_tag="KB953_002106"
     CDS             complement(7222..8037)
                     /locus_tag="KB953_002106"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBB6867071.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(8187..8633)
                     /locus_tag="KB953_002107"
     CDS             complement(8187..8633)
                     /locus_tag="KB953_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HBB6867072.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(8630..9634)
                     /locus_tag="KB953_002108"
     CDS             complement(8630..9634)
                     /locus_tag="KB953_002108"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBB6867073.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(9674..10003)
                     /locus_tag="KB953_002109"
     CDS             complement(9674..10003)
                     /locus_tag="KB953_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HBB6867074.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(10129..12456)
                     /locus_tag="KB953_002110"
     CDS             complement(10129..12456)
                     /locus_tag="KB953_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBB6867075.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
BASE COUNT         3531 a         2440 c         2283 g         4218 t
ORIGIN      
        1 atgcaacctg gaccaataaa aagtaaagaa aaaataaagg aaaagacatt ccttccatct
       61 atcgacaaaa ggaacgcagc tgctatatac aagatattga cacaccaaaa acaaaggcta
      121 cttgccaatg attgatacaa ccaataaaag aattccatat aaacattttt gtcaaaacgc
      181 tgctctttca taatcacctc ctattccttc attcctgatg aagcgatccc ttctacaaaa
      241 tatttttgca tcgctaaaaa tataaaagca acaggaagaa cagaaacaac tgaggcagcc
      301 atgattaatg cgtactctgc atcaaatagc ccaataaaca ttttcaaccc taactggatt
      361 gttttatttt ccgtagaaga caaataaatg aatggcccca tataatcgtt ccatgtatta
      421 acgaatgtga tgatcgtcaa actggcaatc gccggttttg taagaggtaa aatgatcttg
      481 cgatagattc cccactctga taagccatcg atccgcgcac tctcacataa agaatctggg
      541 atactactgt agtattgctt caacaagaac acaccaaaag cattgaacgc ttgtaatagc
      601 accaacgacc acaacgtata tgttaaaccc acttttctca tcataatgaa ctgcgggatc
      661 atataagact gccaagggac agcaatcgtg ccgatatatg ctaaaaataa aatatcgcgc
      721 cctttaaaat ttagtttaga aaacccgtaa gcagcaaaac tagaagtaaa caactgaatc
      781 aacgtgatca caacgcttaa aaaagctgta ttcttaaaaa atgtcactaa aggaatacga
      841 gaccaaataa ccagataatt ctctaaatgc caagttttag ggatccattg aatcggaatc
      901 gtgaacactt cattatttgc tttgaatgaa gcagaaacca tccataaaaa aggaatgatc
      961 gtcaataaag ctaatacaat cagcagaatc atacccaacc attgaataaa gggcatgttt
     1021 tttgaacgaa tttttgcttt ttttctttca tttcgaacac ctccaaagtt ataatcttca
     1081 tttctttgta atgattacaa tccagaacaa cggttattat agctgattca tttccgttct
     1141 tcttatgttt gtaagcgctt atttaacaag ttatgtaaat ctctttattt tttttgattt
     1201 aaaaatgaaa aaatacaaaa gcaagactgt catacgtctc gcttttgtat tgaaatttct
     1261 tcgattagag atacttatta ctgttcaaaa gcttctttga aacggtgaat caattcattg
     1321 actacaggtg ataaaatccg attctttttc cagactagca tgcagttcgt ttcaatcatt
     1381 gggtcaagcg gaacgaatgt cactcgcttt gaaagatgac ttacagcacc ttctatgact
     1441 aaagcagcac ctacctgatt ctcaattagc gcaaatacat tgaaaatcaa attgtacgtt
     1501 cctgtgatca gcaactgctc cttttttatg cctaaccagt cagcaaacat tcgctgtatt
     1561 tctggacgct ttgagatcaa aagaggtaca ttcgcaaagt cttcttttgt tatcacgtct
     1621 ttttttgcta aaacagaatt ttctaaaatg agcaatcccc atttttcctt tcttggtaac
     1681 aaaatcgttt cgtatttctc tgtatctact ggatctagca aaatcgctaa atctaacagg
     1741 cttttttcta gtcactccgt gatatcatca cttgttccgc tgtggatatg aaattgaacg
     1801 ctgggataat cacggatcaa cttttctagt aatagtgcca atgtattcga attatcagct
     1861 tccacacagc cgatcgtaaa tcggccgctg aataattgtt tttttctgtt ctcgaattct
     1921 tgagtggttt gattcgttaa atctaaaatt tcttctgctc ggctttttta gaaaataacc
     1981 tgcttctgtc aagatcattt tgcggttttc gcgtacaaac agttctgttc ctagttcctc
     2041 ttctaactcc ttcagttgtc ggcttaaggt tggttgtgta agatgtagaa cttctgcggc
     2101 ttgagagatt gttccttctt ctgcaattgt ccaaaaatat tttagtaagc gtaactccat
     2161 agcgatgctc cttgattttt ttacttgata cattccattt tatttctaga taacacacga
     2221 tcatatttct tgatttttcc ttgtaaacgt tccaaacact cttgcatttc agcaatttct
     2281 ttcgtcaaaa gttcactttc ttcaatcaaa agcttacgcc gctcaaatac agttgcttct
     2341 ccttcgctag ataggcgaat atattcaatc aaagactcga tcgataatcc ggcatttcgc
     2401 atacagatcg taaagtcgac ccattttagg tcttcttctg tataatcacg gattccgcct
     2461 tttgtccgat taactggtgg aatcaacccg attcgttcat aatagcgcaa tgtatcggcc
     2521 gaaatgccct tttcttcact gacttgtttg atattcatct gatcactctt tctcttaatt
     2581 tcttgccact ttacttatgt attttattta ttctacaact ttaagtgtac tccaagtcaa
     2641 tactcatttc tattaaaact aagctggatt atctatatcg gttgtttgtg tattatttca
     2701 aataaaaaag tcttgttttt ttattctttt ttggatatac ttttaacaat ttagggaaat
     2761 tttaggaaaa ggggtataaa aatgacaacg tatcagcgaa aggtattatt atcgacatcg
     2821 gcgggaattg ctttagagaa tatggatatt atgtttttag ctttttcatt atcttcaatg
     2881 attacttctt ttcatattag tggtacgcaa gcgggtttca tttcaacgat cactaatttg
     2941 gggatgctca ttggaggtat tttttttggc atcatagctg ataaatatgg acgtgtgaaa
     3001 gtattttctc aaacggtcct tctgttttcg attgcttctt tattgatgta ttttgcttca
     3061 aacatctact tggtttatct ctttcgcttt atcgcaggtg taggagcggg aggagaatac
     3121 ggggcgtgta tgtccttgat ttccgaaagt ttttcaaaaa aacaaattgg acgagcttct
     3181 tcggttgctg gtattggtgc gcaggtcggt gcggctttgg ctgcgatttt agctgctgtg
     3241 atcattccaa cgtttggttg gaagatgttg tatgtcgtag gtgtactgcc ggtgttgatg
     3301 gtcttgttca ttcgtcgcgg cttgactgaa ccaaaggaat tcaaatctgt acaatctgcc
     3361 gaaaagaaaa ccaaacttag cagactgttt gaatcaaaac atcttgcttg gcaaacatgt
     3421 ggccttagta tcatggtaac tgtccaaatt gccggctatt ttgggttaat gaattggctg
     3481 ccttcaatta tgcaaaatcg attagggctt tcggtttctg ggtcttcatt gtggatggtc
     3541 agtactattt taggcatgtc tttggggatg atgacttttg gcataatcat ggataaattg
     3601 gggcctaaac tagccttttc tttgttttta atttgttcat ccttatctgt ctatctactt
     3661 gtgttagccc atagccagtg gagtttggta gcagccgctg tgatagtcgg ctactttatc
     3721 aatggcatgt atggtggata tggcgcagtg attagtagtt tgtatccaac agaaatccgc
     3781 gcgacggcga ataatttcat tatgaacctt ggacgagctg ttggtggttt ttcttctatc
     3841 gtcatcggtt tcttgatgga tcattatagt ttaactgccg taatcttgtt tttgagcggt
     3901 atttacctag tcagtctgct ggtcttgaca actcttactg gtgttacagc attaagggaa
     3961 agcttgcagc attgatagtt gagtctgtaa atgacaagaa agaggtgtgg caaaagcttg
     4021 catctgcggt aataagacca gccaaatcaa aaacatgaga cactgtttcc gtttggctgg
     4081 tcgtattact tgatgcaggg cagctttttc aactgctaat ttccttctat ctggcggcga
     4141 ctattctttg acgtccgtca tttcaaagta ctttactaat aacttagcat aacgaaaaac
     4201 tgtggataca ctcgtatatt gagcgtctgc ataatcttcc aaatttttct caggatgttg
     4261 atacatgaat tcaatcaaat gataattata tgattttctg tagaaatgaa gataaataat
     4321 gtagattggg aagttctcct gcttttccat agaaaagcgc cgattattct cattcttcaa
     4381 aatgacctta tcaggatatt tttggaacaa ttcttctaaa tctgttagat aattcaagat
     4441 agacttactt gataccttca attcgttttg tagttcttct attgaacacc aacgttctgt
     4501 ttcaagcagt aaatgaatca ttttaatttt cttttgagaa acttttgtta aaaaagtctg
     4561 catattttct cctcctgtat ctactttact atactacata caagaaaaaa aacggataca
     4621 aatatcatat tgtcacaccg tcaatagtgc ataaacctgt tattatttct ctttttttcc
     4681 taagtattct attgatatga atcatttaat cgtttattat ataaatgtca ataatctaat
     4741 aataaaataa cgtacatatt ttattattgc atacttaata acagaactat agcaaaaaat
     4801 cccgtgtatc aacacgggat tagattaagt ttcctcaatc actattcata acacaattat
     4861 tataacaagt aagtaaacaa ttgaaaagga catttattga aaacggttgt tttttactta
     4921 aggaggaaat ttagcgaaaa aattagttgc attgtttttg gtcgcaggaa gtttgttgag
     4981 tgggtgtggt gctactacgt ctactactca aagcacaagt tcagatcagg cttccagcag
     5041 cagtaccacg atctccgaaa gtacggcaga ttcatcgaaa gaacaagaac aaattgaaaa
     5101 gaaaaaagaa gagaaacaaa aagcggaaaa agctgcacta gaaaaaaagt gacagaagcg
     5161 gaatctgctt tgtcacaagc tgaagcaaat cctacaaaag aaaactatgc aactgcttca
     5221 gcagctatcc aagcgctacc tgaaaacaaa caagagttga caagccgtct tgctgcaatt
     5281 gattccacaa tcaaagcaaa agaagcagaa gaagaacgga aaaagcaaga agaaattgca
     5341 cgacaacaag cagaacaagc gcaacaagaa gcaatcgcac agcaacaagc agaacaggcg
     5401 aggcaagaag caatcgcaca gcaacaagca gaacaggcga ggcaagaagc aatcgcacag
     5461 caacaagcag aacaggcgcg acagcaatca gcagctgaac aggcctctca gcaggcacaa
     5521 gccgcagctc ctgagcaaaa taatgaacaa actgtctatg tcacaccgac cggttcaaaa
     5581 taccatacac ataaatgtgg aaatggtacg tatacccctg ccactttaag ctaagctcaa
     5641 gctcgtggtt taaccccttg ctccaaatgt tatggaaact aattcaaaag ttatactata
     5701 ataataaaat tcttctgaaa cataaaaaaa tagaagatgg cgttccagat agtaaaccaa
     5761 aaataacagt ttacacgtcc atttcgtcag cttctctctt ttttcattta gtctgttccc
     5821 tctgcttttt tggacatctt cattcattct ttctcaatca aatagtacac ctctttttct
     5881 tttcgcagtt tatatcttcc ttttttcaat ttcattgaaa actgtttcga atgtttatgt
     5941 tttacatgga ttttaagcag gagctcctca tgttgcaagc gatagttcct gccttttaaa
     6001 tcagaaaatc tcactagtcc gtattcatca gaagctttca caaatggaac tttttctcta
     6061 gtaatatagt gtttgccgtt gagcgttttt acagaaaaga ccattccttc gattggtcgt
     6121 ccgtttgtgt tctttacata aaaagataaa ggatagtttc gcttagcgat ggccaaagaa
     6181 tctaaataag cgatgagtag ccaaactaag ccgattgatc caagtagtat accgataaac
     6241 caaataaaaa atttccattt tttccattga tcaattcctt ggatcgcttt ttttgcctct
     6301 tttgcttgat aaggaatacg atgccccgtc acaagcagtc gatgtgtatt gaccatataa
     6361 ggagtacagg taagcaaagt cacgagatct ctgccttttt ggatacccaa agaatctact
     6421 tcatcaggta agaccacttg gattttttct acttgatacg ccaatgtctt tccattgatc
     6481 tggatgaaaa actggtctcc ttttttcaac ttaggcaaat ctgtaaataa ttttgcttct
     6541 gggagtccac gatgcccaga aagaacggca tgggtgtttt tcccgccagt cggataagaa
     6601 ctttcttcca ataatgacgt tcctttttgc aaaaatattt ccgttgtctg atcgaatata
     6661 ggtaagctga cattgatttt tgggatttcg atgactccaa gcatatgttc cataaaaaag
     6721 gcattactag gtaaatcttt taatgctttt gcatcaactg cttggttgaa actgcctatc
     6781 cctggtgata cgttttgctc tgccaactgt tggtttttct ttgtcatttt ttcctgctgt
     6841 ttttttattt cctctgaatt cttattcgat gcttgctttt gataatggat gatcatttgc
     6901 tgggcaagaa aatcattcag tgaatcttct acaaaaggat aagaaaagat cccgatccca
     6961 acgactaaaa tgattgccat gaagatgtcc agtattctta ttctgctttt attcatcatg
     7021 tccacctatt ttctaatatg tatatgaaga ggttgtgaaa tcagcgcctt gacctgagta
     7081 ttagcgaagc aaacggcaaa atagttcctc ctattttgcc gtttgttgag cgaataccaa
     7141 gagaccgacc acttgtcaca acctttctcc ccatttgaat cactatcgtt tatttatcgt
     7201 atgatttttg tttccaatag tttaagcttc tgtttcttga tggcgttttt tgaaatagat
     7261 cactgctcct ccaactaaag cagtacctac caagatgaat acgatgatcc ccataccacc
     7321 agttgaaggc aagattcctt taggttggtt atcgatcgtt gtttcatcag aagttgttgg
     7381 atcatcatct actgtaaatt cagtgtacgc agcttcattc ttaggtaatg cgtagccttc
     7441 tggtgctttt gtttcttgaa ggcagtatgc cccttgtttc agcccttcga ttgaaaattt
     7501 cccttctgaa tcagaaatca atttcacttc ttcccagtca gaacctgtga cttcatctga
     7561 ccaagcatat gggtattctc ctgaggcaag gtttgctatt tttttcatta atttaacagt
     7621 tggatctttt gctcttagaa tagaaaattc tgcatcggct aatttttctt gcgtgttcac
     7681 atcttgtttg atgaagtgga aatcaccgta gttttggaca ttttctacag taatcgtgtt
     7741 atccgtagtt gtattttccc gattgactac accgtttgga ttcccaagat tggcttcatc
     7801 tgcgccatgg atgataccgg ttgtttgttg caattttccg ttttttacta taaaagtagt
     7861 ttctttttgt ggattggtta attgataacc atctggtgct tttgtttcaa ctaaagtata
     7921 attgccgtct attaatccat tgacactgat ggatccttca gtatcagttg tgaatgtcaa
     7981 cgctgactca gcaggatcat agagagcgac accagtgttt tcatcaaaac cggtcacgaa
     8041 ttgattctct gaatttttga tctggaattg cgcccctgct aacttatcta cccctgaaaa
     8101 gttcccagct ttctggattt tgatattgct ttctttgatg acatttttag catataggtg
     8161 aatcgtgtct aaaggtttgt cagggtctag ggtaagctta acacgatatt tggcgacgtt
     8221 tcgacaccgg gctttggtgt ttctacaaag acgtatacag catctttccc atcagattta
     8281 cctggaagag tcaggttagc taatccttca ccagaagtcg ttacacttgc gaagggtgta
     8341 gcgtctgctg gaactgctgt gtctttactt ggatagtacg tagcagtgat gtcatatacg
     8401 ttgaattcaa cgccgttcaa cggttcgcta ttcggaaatg tcaattccgt tccgttgttt
     8461 atttgatttt ctggtgctga agtgaatttc aatttatgca aattaaccgt aacattttct
     8521 ggtgtaggtg atacggctgc agaaacctct gcagttccta ggccgagaac gaaaggaagc
     8581 agcatgatag cagttcctaa aaacgtccat atttttgttt tgatgttttt cattttattt
     8641 ccctcctatt gtttcctttt tgttgttttt tataaaacca agctgtccct gttgaaagga
     8701 ctagcaataa ccctaaaatt gagaaacctc caagcatctc ttcgcttgtt tttggaaagc
     8761 ttcctccatt tttttcattt gatggaccat ttgtctgcat ctgaactggc gtttttccat
     8821 tattcgtcgg atttgctttt ggttcgttga tgatttccaa tggtatagga tgaagcggat
     8881 cataactgcc tttttcaact tcaaagagaa tggttgattg actcaatata tatcctttag
     8941 gcggtttaat ctccactagt tcgtaacttc caaaagacaa tccatggaca gaaaatgttc
     9001 catctttact ggaagtaaaa atcgttgctc ttgaatcatc tttggctacc caatgattta
     9061 tcccgttttc ttgcgctaag tattggtcat tttttttgac tagaaattga gcatctgcta
     9121 gtttttgttt cgtttgccga tccactttga caaattgttt gcctcctgtt tggatggcaa
     9181 gatgtgtttc tactgtttga ttgtcagtcg tcaaacgtac atcgttatca aatgtcgttt
     9241 tttcactgtt ttctttcaat gtcatttggt aacggatcgt taattttttt ccagcaaacg
     9301 aactaagatc ttttccagaa tctgataaga caaaaccatg ttttattttt tgtgtgtgaa
     9361 atgtatgaat cttgtctcca ccaatcaaaa aatcgatcgt ttctggttct agccataact
     9421 gaggatcagc tgtatctttg atcgtatagg ttcctatctc gttgatgttc tcaggaattg
     9481 ttacggtgat gagataaggg actttatccc catctgcaaa atctgtctgc ttattctcta
     9541 ctgtttttgt taatgtgagt gcagcaggta ttttttgttc gctctttgga tacaagtgga
     9601 tggtcgtcaa tttatgatta gatgaatctg tcacaggaag cacaacgaca aatggtgctg
     9661 atttttcttt tacttaatct gaagtcttta tttccgtaaa tcggtaaaca gcgtcacgac
     9721 cttgctcatc tttgtccgat gcactaaaag aagcaatgcc ctcttcctcg tctactgttt
     9781 ttgtcacatt ttctgccagt attttttcac tatcactttt ttgtgctagt tttctttgtg
     9841 cgtcttccac actacttcct tctgaccgca gcttgtaaaa ttcatccgag acatcatata
     9901 tagcgaaagt aacgccgttc aaccctttta ctccttgaaa gttatccggg atatttattc
     9961 cgtcgttttg actgatactt ggcatcttat cgctttcgaa cattcgatta tgcaaagtaa
    10021 atgtaatagg tgattctcta gctgacacag tcggagaacc tataaaaaag accagtagga
    10081 taaaaaagag aacagcaaat atatccacac gtacattttt tgtttttttc atcgctgttc
    10141 acctgctttt ttcttacgat ggataaaata aaagaagcca gaaaaaataa agatcagtcc
    10201 taaaaagaca aagggcagta gacctatccc acctgtcgaa ggaagcaaga gagtcggatt
    10261 gatcgtcaac ttagcggtat ctgttgcgat aatcgtataa ttattgttta cacttccttc
    10321 gcctaaacta atcgttacta tttgtgatcc aatatctgtc cggctgttaa ctgttagggt
    10381 ataatccaat gattcgccta agacaacacc ggtaacgtgg agatttccaa ccgaaatcga
    10441 gtgactttgg gcatcataat taaaggcagc gtcatcgaca gtaatcactg cgttagcttt
    10501 atagatggta taagttcctg atgtgttggc tatatcagta aatgacaact tgggatttgc
    10561 tgctttgact ttgtctattc cgctattcgt tagtttaatt gtatagctgc cagcattaac
    10621 catatttttt gctgattgtg catctgctgt gtaatccata ctgctaagtg ccacttgtac
    10681 tggagtaagc gaactagtga caccctctgc tagcttgaca tggattgacg ggacacctgc
    10741 ttttccatca tatgtcttac tgccactgag ctctaccgca ccgctgattt gcttaatcgt
    10801 atagacatca gatgtctgca cgatagtaaa taaatagttt gttctggcag ctagtttagc
    10861 tagtccgctt tttgtcaatt gccaagacgt ataggttcca atttcagtag gcgcagttac
    10921 ttcaactccc ttttcattca tcgctttgaa atcagttgga ttccatgtga tttgatcaat
    10981 actgagatca ttgtttaaaa gggatgttga ttctgtttta tcattaaagg catatttaac
    11041 gatcggtaca acagaaagac catcatagta cttactgcca tttgcattga ttgatactgt
    11101 tgcatttgtc ttataatata ggatgacttc agaaatctgt tttccatttg cgtcctttac
    11161 gattacattt ccatttgtat ctaaactagc taaaacactc attgctgatt ggttctttgt
    11221 ttctatcgct gaaacagctg taaaggtata tccgttgata gtaggcgtat tagtcgtcag
    11281 gtcttgtgca gcacttccgt aagtaagatt cattatttta gaaggagcaa tcatctgtcc
    11341 aaaatagtct tgatacttaa tagtgattgg tgtttgtttg cctgtatagg tcaccgtatc
    11401 gacgttactt tctagggctg tgaaagaacc gtttgtttgt gcaaaagaat agcccgttac
    11461 tgctgttgga gtgataacca ctttgtcacc cacatggatt ccagataatg tagtcccgta
    11521 cgttccgatt tttgctgctc caccattgac tgtataagaa aaagttcctg tccgattcgc
    11581 attgacctga accgttgctg cttgataggt tggaaaattt gaaacatttc ccttaccatc
    11641 aacgatctgc ccatttttga tcgagtaccc cgtgaatgga atatcatttg ctaactgtat
    11701 accgattgaa aaagtactca tacctcctcc ggcaggaagc cctccccatg ttgagggtgt
    11761 aaaagaataa cttttatccg cattgggtgt aactgaatcg atcgtatttc cagcggtagc
    11821 cacccccata tagatacttt ttgggaatgt gatagtagta ccatttactg tggagccaat
    11881 aaacagatta tggatatctg gacggttatc aataaaagcg attgctttgg ttacgccttg
    11941 taacgcagtt ttatccgcac tcatcgatcc gttaaccgca acaatgactc catctttggc
    12001 atatatcact gtatatccat ctggtatggt atgtccgttg aaattcgcag tggccgcaac
    12061 agtactaaat gtctggcttc tacctgtttc tgaataagct ttctcccctg ctgcttttgc
    12121 tgctgtgatt tctttttctg tcggatcaat cagttgagtc gaggatgtgg ttgaggttgt
    12181 ctcgttgttg ctagactgat tggtgctttc tatggactgt atttcagaat agggtggact
    12241 atttgtctcc tctgtcgttt ggttagcaaa atttgacgtt tctgtataaa cgtttacata
    12301 aatacttgat aagctcccta agaaagtaaa aatcaagatg atcgaaatca taatgggatg
    12361 taagtatttt cttttagata cagctttgag tctagaaaca aaatgttttt gacatatacg
    12421 ttggatttta ttttgagttt tcttaatcat tttcatccct cctctcaata aa
//
LOCUS       DADVXU010000080        12080 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.084, whole genome shotgun sequence.
ACCESSION   DADVXU010000080 DADVXU010000000
VERSION     DADVXU010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.084"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(38..934)
                     /locus_tag="KB953_002111"
     CDS             complement(38..934)
                     /locus_tag="KB953_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBB6867076.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(927..1607)
                     /locus_tag="KB953_002112"
     CDS             complement(927..1607)
                     /locus_tag="KB953_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBB6867077.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(1608..2390)
                     /locus_tag="KB953_002113"
     CDS             complement(1608..2390)
                     /locus_tag="KB953_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6867078.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(2383..3300)
                     /locus_tag="KB953_002114"
     CDS             complement(2383..3300)
                     /locus_tag="KB953_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBB6867079.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(3464..3787)
                     /locus_tag="KB953_002115"
     CDS             complement(3464..3787)
                     /locus_tag="KB953_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867080.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            4575..7802
                     /gene="fss3"
                     /locus_tag="KB953_002116"
     CDS             4575..7802
                     /gene="fss3"
                     /locus_tag="KB953_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HBB6867081.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            7915..8229
                     /locus_tag="KB953_002117"
     CDS             7915..8229
                     /locus_tag="KB953_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBB6867082.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            8242..8616
                     /locus_tag="KB953_002118"
     CDS             8242..8616
                     /locus_tag="KB953_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBB6867083.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            8626..8961
                     /locus_tag="KB953_002119"
     CDS             8626..8961
                     /locus_tag="KB953_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HBB6867084.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            9044..10384
                     /locus_tag="KB953_002120"
     CDS             9044..10384
                     /locus_tag="KB953_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HBB6867085.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            10462..11136
                     /locus_tag="KB953_002121"
     CDS             10462..11136
                     /locus_tag="KB953_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HBB6867086.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            11129..11293
                     /locus_tag="KB953_002122"
     CDS             11129..11293
                     /locus_tag="KB953_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867087.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            11369..11803
                     /gene="dcm"
                     /locus_tag="KB953_002123"
     CDS             11369..11803
                     /gene="dcm"
                     /locus_tag="KB953_002123"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HBB6867088.1"
                     /translation="MRFLDLFAGIGGFRLGMEQASHHCIGFCEIDKFARRSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQRFSDTRGTLFFEIARF
                     TTLLRPKLLFLENVRGLLNHEGGATFETILRTLDGLGYDVEW"
     gene            11804..>12080
                     /locus_tag="KB953_002124"
     CDS             11804..>12080
                     /locus_tag="KB953_002124"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HBB6867089.1"
                     /translation="MLNSKAYIPQSRERIFLIGHSRDACTEQVFPIIGPSSTSDQNIR
                     NLVNINPSNRGMGGQVYGSDGVAPTLTGDEGIKIALPARDGISVAGLL"
BASE COUNT         3846 a         2247 c         2467 g         3520 t
ORIGIN      
        1 atacattttt ctactagttc tcactaatct tgtataatta aaccctccgc aacttgacgg
       61 agatggagaa gatgcccccg tctaatctag cggtcatctc tcctcccatt ccttccacca
      121 atttttttga caggtaaagc ccgattccgc tagattccgt atttgtgcgg ctggtatttt
      181 cggtataaaa acgctggaag actttttcaa cagaggtttg cgttcctggt ttgataggat
      241 tggccactgt taatcgaatg aatggatcct cttttgaaaa tgtcaccttt gcttgtccat
      301 ctgcataacg caataaattc tgtatgatat tttgcagcac tcgcagcaac atgagcggat
      361 ctgctaatac agttgtattt atcgagtgtt cagcgaacgc tacctcgata tttttctcag
      421 aaaattgttc ataaaaagag aaaaatactt cttccacctc tttagaaaaa gaaagcgtat
      481 ggagatccat ttctttttgc ttcgtctcta ttcgagcaag atcataaaac tgatccgtca
      541 tactgagtaa tcttgcaaca gattgtttga tgatcgttaa atacttctcc cgtttctccg
      601 tatcctctac ctgttccagt aattgtacat aaccgttaat agcagtcaaa ggagttctga
      661 gatcatgaga aaggccgacg atcgatagtc tgatgttttc ttccatttct tgaaatgctt
      721 gtcctttttg ttcattttca tccaaccatg tattcagtgc atctactagg tccattagat
      781 tcttttctcg aaattcgact gaaagacgac caccatattc ttgtcgttca gaaagagtgg
      841 cgattttctt agttaatctc ttgatttccc tttgcaggaa gaaaataaaa cttcccataa
      901 agataacaac gagaagcaac agccatttaa tcaaatctaa accccactcc ccacaccgta
      961 tgaatataca cagcatgtgc ctttgctagc ttccctcgaa gattggaaat atgcatattc
     1021 actgttttct cactatcaaa atatggttct tgccaaactg tttcataaag atttgctcgc
     1081 gaaaatactt ttttcggatg ttctaaaaaa agcttcagca gatcgaattc tcttcctgtc
     1141 aaatggacag ccacaccatt cacatttaca tccctcgact ctttgtccag taagatttcc
     1201 ttgtaataga tgcgttgcga cagatctttt acttggacag aagtctgatg acgaagttgg
     1261 atgccgattc gtaacaaaag ttcttcgata tcaaatggtt tcgtcacata gtcatccgct
     1321 cctgctgcca acaaggaaac tttatctctt tgatcgcttt ttgctgaaag aacgatgatc
     1381 ggaacattcg atgtctgtcg gatttctgct aacaatgctt cacctgaaag ccctggaagc
     1441 atgagatcta gaaggatcaa atcgacagga tatgtgctta acaataattt tccttccgta
     1501 cctgaataag catcaagaag tttgtatctc tgttcaagaa cctcctccag taaggaatga
     1561 acagcttcat cgtcttcgat aatcaaaata gtttccagca ggaccaccta cttaatgtca
     1621 gatttttgga aaataaacat tcctagagct gatgatacca cgattgttac tagactgacc
     1681 agaatcattt tcggtgcttg cgaataagtt gcttgttgcg caaagtccgt atacatgagc
     1741 cacgtttccg taaaccactc cgttagaaat tccaagcctt tgatttttgc gaaaacaccg
     1801 ggaatgaagg ccgttcccat gatataaaga aaagaaagaa gcaaactgac actggtactt
     1861 ctagtgatga atgtcaaagc cactggaaca gataatgtcc ctaatgtcag cagaagcaat
     1921 acaggaaata cttttagaac ataatttcca tagtttttga ttccctcaac ttcaccaaat
     1981 ccgaaagcaa gtgtgtaagc aagcatggct agtcccgtga tcaaaaccac gccaagtgtt
     2041 gtgatcaaaa gcgtaacgag aagtttcgac acataataag tcattcgatt ccgtcctaaa
     2101 gataacgtat tgcgaatcgt accgttttga aattcttccc caatataaaa gcacgcaaat
     2161 gctgcgaaag ccagaagaat ccatgcagcc attttttcta ccattaaaat ccctgttgca
     2221 ccgttgatga ctgtatccgt tgcttggttt aattcattgc cgtattttgt atgcatatac
     2281 cattcgttcc aagaataaaa aagcggaagt acgaccatca gtcctagtga gacccacatt
     2341 ttacggctct tccatacttt tattttttca gcagttaata gattaaacat gtggattgcc
     2401 tccagttaat tgtaagaaat aatcttccag tttttgccca actaggtgaa taccatccac
     2461 aggaactttt gcttctacca atgcttgatt gatggctgct acttggtcaa ttccttcaaa
     2521 aagctggata acacggctag attgtacaaa ataatcccta taaccaagag agtcgagaac
     2581 cgttactgct tgttccgtcg ccgaagtgtc aatttttata aattgacgag attcttgtgc
     2641 caattcttct ttcgataatt ctttgatcaa gtgtccttca tgcaaaatac catagtgagt
     2701 ggcgatctgt gacaattcat ctagcaagtg gctagaaagc aagacggtga tgccccgttc
     2761 ttttaccaaa cgttggatga tttctcgcat ttctgccatc cctgctggat ccatcccatt
     2821 cgtcggttca tccaaaatca aaaattctgg attcgtgatc agtgcatttg caatagctaa
     2881 acgctggcgc atacctaaag agaaattctt tgctttcttt ttccctgtat tttccaatcg
     2941 catcattttc agcaggtctt cgatttctct atccgacaca ccgccgttcg ctgcttggat
     3001 acgtaggtta tctcttgcag ttaattctgg ataaagagca ggggtttcaa tcgattgacc
     3061 catacgtcgt cttgctgcct gcaaatccgt cgttccaaat aattcgatcg aaccttcatc
     3121 aatcgagatc aaacccataa tagttctcat aaaggtagac ttacccgctc cattttcccc
     3181 aatcaatcca tagatcattc cacgtttgat ttcgattgat actttatcta atgctttggc
     3241 agcaccgtat tttttggtta taccagttgc tttcaagacc gtttcatttc caaaatccat
     3301 acttatcctt ctttcttatt gatgagtgaa gtatagttga cgggtataaa gaaagcagcc
     3361 agaaatgtat aaagaaagtg taaattttcc aattgaatgt caaaaaaaac aatcggacaa
     3421 tacgtgtctt tcctattatc aggcagtttg ttatacgctt gtatcagttt cctgattctt
     3481 tcttttcgac caaagtcgtt tctctgtatt cctcttcact gaaaattttt gcttctttca
     3541 gatcaagctt gagtaatttt accagaccgg ttataaggat aactatagca agactccctc
     3601 ccagaaaaat tagactgtct cgcagacgtg gttgcgttgt cttccaatca aagagcgcac
     3661 caaacagtgc taatccaagt ggactggcca ccatggtaat cgttgttatc gttgacataa
     3721 gtcgtccttg tatatgcgtt ggaacttctc gctgaagcca gaccatataa aaagcaaaag
     3781 aaaacattaa tccttaaaaa cggacttatg aaaaataata catgccgttt gggatttaca
     3841 attagctacc aaataagagg ttatgacaaa agacttttca caatcccttc ttccgaataa
     3901 acggtgttct cctgccaact cctcggtcac aagtcacaat atttatcaaa catgagaagc
     3961 aatttttgta aattccttct tatttcttga agctgaccac ttctgtcaca acctcagaag
     4021 cggataaata atattgtttg atgatacttt atctttttcc cgtttgttta tagatcgaat
     4081 gatattatag accaagaatc ctacctatta acatagctaa aaacacaaaa aaggcacttt
     4141 ccaaattgtg gaaagtgcca gaaaagttag gattaaggta actgcgctca gcattgcttc
     4201 gcccagtacg attctcgtaa ctcgtgaact cgaacgataa tcgcaattaa cgttttgaga
     4261 actgtgaagc tttacgggct ttcttaagac ctggtttttt cttcgtatca ttgaataagt
     4321 ttgaaaaacc ttgatttata aaggttttcc cgtaaatata ttttattata tttgattaaa
     4381 tttgaaatgt ttgtgttcta aaatgtgttc taatttaaag gcagtatatt tcgtatttta
     4441 gattaaattt ttaggctcat tcattctcaa atccattcta ttcagtcaat aaaaatttta
     4501 aaaagacttt ataaacaaag atgttcaagc gagcatctta tttttttgtg cttaatttga
     4561 aaggagaaca tttcatgtat aaaaaacaaa aaaagaaatt atgtgtatta actattttga
     4621 gtttggctgt tcaagcaatt gttggagcaa ttgctcctac gattactttt gctgatgaaa
     4681 ttacccatcc acaaacggta accgtagaac tagatttagc acatcaatat gctgttgaag
     4741 gaacttttag tgatggtcgt ccaatgtcag aagtcactgt tccgcattat gcagtgtaca
     4801 acggctttaa acaagatatt ttctgtattg aaccaggagt cccgatttac aatgagttca
     4861 ctcctggcta tgagaaaaac ccgttaccag atatgtccga taaagccaaa ttagtttccg
     4921 ttttatggaa aaaagtaggt acagatgcag atactcatat tgtggcacag aagatgatct
     4981 ggcaagaagt taatgggtat acccttcact caataaaacg atcagatggt agtgccgtaa
     5041 atatcgcagc cattgaggca aaaataaatc aagccattga cgattatcag aagaaaccaa
     5101 ccttccataa tagtacggct aaaacagtat tagggcaatc gactactgtg acggatacga
     5161 ataatctgaa tttgtcagag ttcgatgagg ttgtggaaaa tgccgcaaac attgattatc
     5221 gtgtgaatgg taatcagttg ataatcaccc caaatgccaa ttcaaaagaa agtggtgtgc
     5281 tgacgcttaa gaaatctgct ggtaccggaa cacctgtcgc ctataaaaag gctggccaac
     5341 agactttaat ggccggggcg attgataaac ctaataccta cacaatcaaa attgatgtgg
     5401 aaacggaggg ttctttaaag atcaaaaaag tcgataaaga atcgggtgat gttgtaccag
     5461 gaacggtttt ccatttagac tttggaaaaa ctttacctgc aaaagacgtg actactgaca
     5521 aagaaggcat tgctacattg gatgagattc ctcatggtac aaaagtaacc attaccgaaa
     5581 aatcagtacc ggcgccttat acgattgata ccacccccat gactaccacc attaaagcag
     5641 gcgaaaccat ttacgtgact tcaaaaaacg cacgggaaaa agggcaaatc attctggata
     5701 aaagcggagt tgaaacaggg agtgatcttt ggaatgacaa ttattcctta gctggtaata
     5761 cgtttgccat tcgcaaagac agtccaaccg gtgaaatcgt tcaagaaatg accacggatg
     5821 aaaacggtca tgcggaaaca ccaaaagaga ttgctaatgc gttggaattg ggaacctatt
     5881 atgtgaccga aactaaagct agtcatgggt tcgtgaatac gttcaaacct gtaaaagtcg
     5941 aattgaagta tgccaatcaa accgtagccc ctgttaccag taacgtaaaa gggcaaaatc
     6001 aagaagtcac tggcgaaacc actttgacga aagaagacaa agatactggg gataaagcac
     6061 aaggtaaggc agtctttgag ggtgctgaat acaccttgtt tactgctaag gatggaaaag
     6121 ctgttaaatg gagtgaggct tttaaaccag agttagcgaa gggaacgaaa ggttctgatg
     6181 aaacggtcac tttgacttta gacgaaaaga accaagttgc cgttaaacat ttagctatca
     6241 acgagtatta ttggcaagaa acaaaagcgc ccgaaggata tagccttgat gaaacaaagt
     6301 atcctgtatc catcaaaaag gtggatgata gcgaaaaaaa tgccgtgatt acccgaaatg
     6361 ttacggcaaa agaacaagtg attcgctttg gctttgattt ctttaaattt gctggatcag
     6421 ctgcgggcac taccgaaact ggcttcaatg atctgacctt taaagtgtcg ccattggaag
     6481 ggaccaatga aatcacaggt gccgaagatg aagcgaccac agcttataac gagcaattag
     6541 ggtttgatgg ttatggcaag tttgaaaatc ttccttatgg ggattattta cttgaagaag
     6601 tagaagcccc agaaggtttt caaaagatta cgccattaga aatccgttct acttttaagg
     6661 aaaacaaaga agactttgtg aagagtgagt atgtctttac cattaccgaa caagaccaaa
     6721 aacaaccaat taaaacggtg accgttcctt acgagaagct gacgaacaaa gcattttctg
     6781 ttagtttgaa ccgtttaatg ctctatgatt tgcctgagga agaagatagt ttaacttctc
     6841 ttgcgacttg gaaagacgga aacaaagaat taacttctct ggattctacc gagctagttg
     6901 ataaattgag ttacaatcta catgaaatca aagaagactg gtatgtggta gcgcaagcca
     6961 ttgatgtgga tgcgacaaaa gctgcccaag aaaaagatga aaaagccaaa ccagtggtga
     7021 ttgcagaaac aagcgcaacg ttggcgaaca aagagaaaac aggaacttgg aaaattcagc
     7081 ataaattaac cgccgaacaa gttttaaata aaactattgt cttattcaac tatgtgtatg
     7141 aaaataagga agcttttgaa gcaggcgata agccagtagc gaaggatgtt agcttgaata
     7201 accaagccca aactgtcagt tgtacagtgg aacaccatgt ttctatccaa acaaaggccc
     7261 acctagaaaa cggttctcag acctttactc atggtgatgt ggtggatatg tttgatgatg
     7321 tgtcgattac tcatgatgta ctggatggct caaaagaagc tttcgaaaca attctgtatg
     7381 ctttattacc ggatggtaca aacaaagaaa tttggaaatc tggcaagatt gattatgaag
     7441 taaatgataa agaattcacc aaaactgtgc ttgcgaaaaa agtagatacc ggaaaatatc
     7501 cagaaggaac caagtttact tttgcggaaa tcaattacga taaagatggg aatatcaatg
     7561 gaaaacacaa tgaagatttg aaagaaaaat ctcaaacctt aacaccaaaa gaagttccaa
     7621 ccatactaag tacgccgaaa caaccggaaa caccaactgt tccaagtggc tctcaagaat
     7681 ctagtcccac agtgaagacc ttccctcaaa ccggggagaa aaattccaat gttttactgt
     7741 tcattggttt tactttgatt tttgcgacgg cgggctatta tttctggaat cgccggaact
     7801 aaggtgatga gatgatacaa gtaaatccta agaaccaacg tgcgcgaccg cctcctaaaa
     7861 aagtagtcgc gctatttttg tatccaaaac taacaatgaa aagaggaaaa gaagatggaa
     7921 ttgaaatttg tcgtgccaga tatggctgaa acctttggaa agttaagtta tgccggtgaa
     7981 ggagaaatta taacagaagg atatggacgg aatactacgg tgattggtcg cagttatcat
     8041 ttgtattcca gtaagcaacg ggccgatgat attgaggtgg tagtagctgc ggaagccggt
     8101 gaaaaggatt ttgatcaaga ccaaccgcta aaagccgtga atccccattt ggttgccaaa
     8161 ggctatgaga ttgagaatcg tgggtttact gattacgtgt tgtatgttga tgatttagtg
     8221 aaagcatagg aggaaaagaa gatgagatta acagaaggta ttgtcgtaga ttcaggttta
     8281 acatttggga agttgcgttt ttccgcatta cgccgagaag tacggaagca aaatgaggat
     8341 ggaacaattg gtaatgaggt aaaagaacga acctataatt taaaatcctc tgcacaaggg
     8401 cgaatgattc aagttagtat cccagctaat gtacccttac gcgagtttgc ttatgatgca
     8461 gaggtagagt tggtcaatcc aatcgtggat accgttgcca attatgtttt tcgagaagga
     8521 actaccgtta attggttcat taaggcggat gatttagtct tgaagcggca gccgaatcaa
     8581 ggaaatccta cgaaccaaaa tgaaagaaag aaataggtga agattatgga aattaatgtt
     8641 gaacatattt tagattgtct cgatcaatat ggaaaaggcg agctgactga ggaacaactc
     8701 gttaaagcat taacctctga tgaaaaaatg tttctgatta tacatcaagg tttacttgga
     8761 gaaaatagcg acagtgaaga agactctgat gtactagact ggttaggaga agaagagtct
     8821 ttctttatag ttgtcgaagt aaatgaaaac ttatgtcggc aggcagaatc cattttggaa
     8881 gaaattggtg tggagatgcc ggatgctatt gaaggctttt taaatcaatt ggttgaaacg
     8941 aaacaactcc cctcctcgta gcagtaaatg actagcataa ctttttgaaa ttgcctaatg
     9001 aaatcctatt gggctttttc gattggaggt ggaaaccatc aaaatgtaca aaggacatcg
     9061 catacgcgca ggagatcagc acttggttta tcactttgtt cttggctggc tactcgctct
     9121 gttcatcggt tggatgagcg tcttttattt tcaagagttt agacaatttg atatttctaa
     9181 gctatcactt tctactattg aaatcgtttg gtctataaaa gatttagtat gtctgcttgg
     9241 aagtctcgct ttttcaggag ctatgatatt gctttatatt cacttttttc ttgatcattg
     9301 gcgaagcttg tggcatcgac aaaagctagc tcggatgatt ctagagaacc attggtatga
     9361 agtgaaacaa actcaaagtg aaggcttttt caaagatttg aatagtagcc gaaccagaga
     9421 gactatcagt tacttcccca aaatctgtta tcgaatgaaa gatggattac tgtctattcg
     9481 cgttcaaatt tcacttggga aataccaaga tcagctttta aaattggaaa agaagttaga
     9541 aagcggactg tattgtgagt tagtagaaaa ggaactcaaa gactcctatg tggagtatac
     9601 tttactgtat gatatgatcg ccaatcgaat tgggatagac gaagtagtgg cagaaaatgg
     9661 gactctgcga ttgatgaaaa atcaagtatg ggcttatgat tctttacccc atatgttaat
     9721 cgctggtggc acgggtggag ggaagaccta tttcctcctc accattatcg aagcactatt
     9781 gaagtcagat gcagaattgt ttatcctcga tcccaaaaat gcagatttag ctgatttagg
     9841 tacggtgatg cctcatgttt attcacaaaa ggaagaaatt tctgcttgtg tggaagattt
     9901 ctatgaacgt atgatggctc gtagcaaggc aatgaaagaa atgtctaact acaaaacagg
     9961 agagaattat gcctatcttg gacttccacc caacttttta atctttgatg aatacgtggc
    10021 ttatatggag atgttaacga cgaaagaaag tgcggtgatt ttgaataagc tcaaacaaat
    10081 cgtgatgttg ggtcgtcaat ctggtttctt tctgattttg gcttgccaaa ggccggatgc
    10141 gaaatattta ggagacggaa tccgcgatca attcaacttt cgcgtggctt tgggtcgtat
    10201 gagtgaactc ggatattcca tgatgtttgg ggaggtcgaa aaaaacttct ttatgaagca
    10261 catcaaaggt cgaggttatg tggatacagg tggcagtgtg attagtgagt tttatacgcc
    10321 acttgtacca aaagggtatg atttcttaga ttcaattagt aaattagaaa agatgaatga
    10381 ttgagtgatc ttcaatactg actttatact tcaagtaacg tataatactt ttatatagta
    10441 ggaagaaagg ggataattaa tatggaaagt aatgattctt atttagagta tgcaaaagca
    10501 aataaggata agtttatttc ttatattatc aaagggaaat cccccgaagt tggacgagac
    10561 gcagttttta tggcagggag tcctggggct ggaaaatcag aggttgccat tggattagct
    10621 gagaattatg ataaccatgt gattattgat gcggattact ttcgcaccca gtttcctgat
    10681 tataatggaa aaaactctag tctctttcaa aaagcatctt cttggttggt agagcaggca
    10741 tttagatatg tcttagatca tggctattct tttattctgg atgcaacttt tgcaatatta
    10801 agtgctgaaa aaaatatctc gcgggcgttg aaaaaacatt ttcgagtgac gattttctat
    10861 gtttatcaag accctaaaat cgcttggaaa tttacacaag aacgtgaaat tgcagaggga
    10921 cgtcatgtgc cgaaaaaggc atttattaac gcttttttca aatcaagaga gaacattgca
    10981 aaagtaaaga gtaaacatcc agatgtattg cttcatatca taataaaaga ttatacgaat
    11041 aatatttccg aagttcatta tgctacggat aatctagaat tatttttacc actagggtat
    11101 agtaggaaag atttggaggt tcaattacat gactgagaaa gaacaagtaa cgaaaattgt
    11161 aaagaaatac aataagagca ttgcggattt atcggaaaat gctactgcaa aagagttcaa
    11221 aacggtgatt aaatatgtag ccgatcaggc caatgaaaaa caacggaaat tagttggatt
    11281 aaataaaaaa tagtaggaca aattaataat gtatgagcca agagcaaaat gttcttggct
    11341 tttttctttg caatgaatga ggtaatgtat gagattttta gatttattcg ccggtattgg
    11401 tggctttcgt ttaggtatgg aacaggcgag ccatcattgt atcggttttt gtgaaattga
    11461 taaatttgct agacgtagtt ataaagcaat tcatgataca agtaaggagg tggaaatgca
    11521 tgacatcaca agtgtatcag atgagtttat tcaatccctc ggaccagtgg acatcctttg
    11581 cggcggattt ccgtgccaag ctttttcaat tgcgggaaag cggcaaagat tttccgatac
    11641 tcgaggtact ttattctttg aaatcgctcg gttcaccact cttctccgac ctaagctttt
    11701 attccttgag aacgtccgag gattgctcaa tcacgaagga ggggctacgt tcgagacgat
    11761 cctccgaacg ctggatggat tggggtatga cgtggagtgg taggtgctta actcaaaggc
    11821 ctacattccc caaagccggg agcggatatt ccttatcgga cattctcgag acgcatgtac
    11881 cgaacaagta tttcctatca ttggaccgtc ttcaacatct gatcaaaaca tcagaaattt
    11941 ggtaaatatc aatccttcca atcgaggaat gggtggacaa gtctatggtt cagatggcgt
    12001 agcgccaacc ttaacaggtg atgaaggaat caaaatcgca ctgccagcaa gggatggaat
    12061 ctctgtagca ggactattac
//
LOCUS       DADVXU010000081        11831 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.085, whole genome shotgun sequence.
ACCESSION   DADVXU010000081 DADVXU010000000
VERSION     DADVXU010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.085"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(19..225)
                     /locus_tag="KB953_002125"
     CDS             complement(19..225)
                     /locus_tag="KB953_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867090.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(241..855)
                     /locus_tag="KB953_002126"
     CDS             complement(241..855)
                     /locus_tag="KB953_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBB6867091.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(1002..1685)
                     /locus_tag="KB953_002127"
     CDS             complement(1002..1685)
                     /locus_tag="KB953_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBB6867092.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1794..2252)
                     /locus_tag="KB953_002128"
     CDS             complement(1794..2252)
                     /locus_tag="KB953_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HBB6867093.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2336..4099)
                     /locus_tag="KB953_002129"
     CDS             complement(2336..4099)
                     /locus_tag="KB953_002129"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBB6867094.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4320..5780)
                     /locus_tag="KB953_002130"
     CDS             complement(4320..5780)
                     /locus_tag="KB953_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867095.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(5988..6914)
                     /gene="glsA"
                     /locus_tag="KB953_002131"
     CDS             complement(5988..6914)
                     /gene="glsA"
                     /locus_tag="KB953_002131"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBB6867096.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6937..8364)
                     /locus_tag="KB953_002132"
     CDS             complement(6937..8364)
                     /locus_tag="KB953_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBB6867097.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8395..9609)
                     /locus_tag="KB953_002133"
     CDS             complement(8395..9609)
                     /locus_tag="KB953_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HBB6867098.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(9950..10192)
                     /locus_tag="KB953_002134"
     CDS             complement(9950..10192)
                     /locus_tag="KB953_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBB6867099.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(10224..10781)
                     /locus_tag="KB953_002135"
     CDS             complement(10224..10781)
                     /locus_tag="KB953_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBB6867100.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(10794..10982)
                     /locus_tag="KB953_002136"
     CDS             complement(10794..10982)
                     /locus_tag="KB953_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HBB6867101.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(10995..11561)
                     /gene="amaP"
                     /locus_tag="KB953_002137"
     CDS             complement(10995..11561)
                     /gene="amaP"
                     /locus_tag="KB953_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HBB6867102.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         3472 a         2449 c         1936 g         3974 t
ORIGIN      
        1 acttttcgga ataaatattt atttgttaaa gtaattaaac aatccgctaa tcgtttgatc
       61 tggcatttct atttccttac catctaaagt aacagtagta acgacaaaat ggtcttgcaa
      121 tggcggaact tctaagtact gcccttgcag aaaatcattc actgtttcat attctttttt
      181 catttctttt gaatctgtcg atggtttata gcttattttt aacataacta gtgctccttt
      241 ttattcggga cgtgcaatcg tgaaaatttc tcctattgct gcataatcat ttcctgccaa
      301 acggcttaca gcattcaatt ttcttggatc gatccttcct gtcttatcat catagactgt
      361 ttcatttaaa tgatatttct gtacttctag caaaaataaa tcagctgtta ctttattttt
      421 attccgtatc tcgacatgtt catataagga tgtttcaaaa cgaacttttg cttctgtcaa
      481 tgctggtact gaaatgatta ctgaatcagt taaagtcaag tcagtcaggt acagttcgct
      541 ttcctcatta ggcagattgg ctgctgtttt atttacttct tctacgttgt cctcatcaag
      601 gatatggata acagcttctc ttgtctcaag aatattcttt gctgtgtctt ttactgcatc
      661 ttgttttctc tggatcgcca aggataagat gggtcgattc gaactgacga tattgaagta
      721 actgaagggt gcgatattca ctatatcttt tgctgattta gtcgttacta ctgctactgg
      781 tctagggatg actgaaccaa tcagcaactt gtaattttca cgttctgtca attctgaagg
      841 actccgttca aacatggttt cgccttcttt ctataaaatt cgttaaaaag cagatcttga
      901 agacaaaatg aaaagtaatg cttttttcat cttcttagaa tctgctttaa actatctatc
      961 aatttttctt ttttgtcaag aatctgtgcc gtgcgtgttt tttagttatt tgctttactt
     1021 gcggcttcga catatctttt agtgatcaat tgcggacggg tgataataga tccaactacg
     1081 actgaaaaag cacctaattc atatgctttc tctacttgtt caggagagtc agtatgtcct
     1141 tccataatta ctgggatatc gacttgttct atgatttgat tcaataaatc aaaatcaggt
     1201 gtctgcgtct ttcgtgaata ttctgtatag cctgctaaag tcgtagacaa aagatcaaaa
     1261 ccatcttctt gcgcttgaag cgcttcctca aaagtcgaaa tatctgccat agctaaccga
     1321 ccgtgttcgt gaatcatttg gacaagttca gcagttgttt tttctgtcgg acgtttacga
     1381 gaagttgcat ccagtgcaat gatttctgct tgcgtctcca aaagttcttc cacttctttt
     1441 tctgtcgcag taataaacac ttctgaccca tcgtaatctc ttttgataat ccctattacc
     1501 ggtaaatcaa cgattcgctt gatttcattg atatcttctg tcgtatttgc tcgaatacca
     1561 actgctccgc cttcttttgc agccaaagcc attctcccca taataaaaga actgtgtagc
     1621 ggctcgtttt ccaaagcttg acaagaaacg acgactccgc ctttgatttg ttccagtgta
     1681 tccacaatat ctcctccagt aatgttatat aaaaatacag cttcatataa aaatgtaacc
     1741 cttttctatt caggagacaa taatgagcta tttctttcag cagacctttc ttactaaaag
     1801 aaaattactt caaattcttc gtatacgacc agcttctctt ctgtaaattc tatgttcaac
     1861 gcttctttat aagcatgttt aaaaaactca gcatggactt tccgccaata agctaatgtc
     1921 cgatcccctt ctccttcttt ccaagcatgc tctttcgtga cttcactaaa aggcaagatg
     1981 gtcacattta tatttttgat gatacaaact ggttcatttt ttccattcag gaccacgtct
     2041 aattgaccaa cttttggtaa aggctcattc tcttgtttat acagatcgta accacttgat
     2101 gtaccagtct ttatcttttt tacaactagt tcgctcaact cgtctgccat tttttctgta
     2161 tccccaaatg aaaagaaacc taatttttct gattgataac taggatattt ttcttggaac
     2221 tttttgacat attcttctac ttgctgatac ataacatcca cccctttgct ttttataatg
     2281 aaaaaaccat ccagatacta gtataaacaa ttatctggat ggtgagcata atgaattatt
     2341 gaatattcaa gacacgcaga ctatcttcaa cgatttgttt tggtgttaaa tgataacgtt
     2401 cataaagctg ccctacagga acgctatccg tgaattcttt ctctgcacca aaatttaata
     2461 cgttcattga agaatcacca taataacgac tgatattttc gccaaatcct cctgacaaac
     2521 taccatcttc taatgtgatg actaattcgt gtgtttcttt taatttatct aatgtttcta
     2581 cgtcattttc tgtgatcgtt cctggattga tcaacgttgc ttcgattcct ttttttctaa
     2641 aagcttttac tgtttctttt cctaattcaa gaaaaccgcc taaagctaaa attgctactt
     2701 ttcctccatt atttactgtt tgataggctg gaatactata atcatttgcg atagctgtac
     2761 catgttctac accatgtgta ggaattcgga ttgctacagg ctgcgctgtt tgttcgaacg
     2821 cccaatctag catagagatc atatcctctt ttgttgctgg agcaagatat ttgatgttcg
     2881 ggatacttga taagtaagga atgtcaaatg ttccttggtg tgttcggtca ctagctgaga
     2941 tcgttccgcc accgactaaa ataatcactg gattctcgtt gatcgctaaa tcatgtgata
     3001 attgatcata tgcccgttgc aagaaagtac ttgattggaa gacgactgga cgtactccct
     3061 gagaagccaa agcagaagcg aaagtgatag agtgctgttc agcgatcccc acgtcaaaat
     3121 aatgatctgg atgtttttct tggaatgttt tcagtccaaa ggcgcctggt atcgctgcat
     3181 tgactgcggc aataggagct gtacttgttt gcattttctt atccaaataa tccatgatga
     3241 tcatattaaa tgtttctcca gaagttactt gctttgcctc cccggtggac agatcaaatg
     3301 gtacatgcca atgatatttc attttattat cgattgccgg ctgataaccg cgaccttttt
     3361 ccgtatggac gtgaagtaca atcggatgat caatatcttt tacttcttca aataaacgga
     3421 tcaccgtttc cagatcgttt ccattatcca cgtaacgata atcaaatccc attgctttaa
     3481 ataggttatt gctgctttta ccgttggttt gacgaagctc agctaagttt ttatataaac
     3541 tgccatgatt ctcatcaata gacatctcat tatcgtttac taatacgatt agattggatt
     3601 gtaaaacacc accattgttc aatccttcaa aagctaagcc accacttagg gagccgtcac
     3661 cgataaatgc taccacattt cctgattctt tcttcagatc tctcgcttta gctaaacctg
     3721 ttgctaagct gatagatgta gatgtgtgcc ccactgtaaa atagtcatgt ttactttcat
     3781 gttggttgga atatccgctc acttcacggt aatgtccttt ttcaaaacca ttttttcgac
     3841 cagttaaaag cttatgacca taagactgat gggaaacatc ccaaacgatt ttatcctttg
     3901 gtgaatcaaa tacataatga aacgcaatcg ttaattctac tacacctaga ttcggaccaa
     3961 cgtgcccacc tacatcgctg tccttttgca agattttttg gcgcatctct tctgccaact
     4021 gtttcatttc ctcaacagat aattttttga catctgcggg gctattgatt gcatccaata
     4081 catgtaatac tgtactcata atacattctc tcctccgtac tatcttcttt taatgaaaat
     4141 agatttgtta cactttatct attctactac ttagagttga ctctaagtca atagcatttt
     4201 aaaaaaattt cagagaaagc ttgttatata agtagatctt cgcacgaatc aggactcaaa
     4261 taaaaacctt ctacagacaa ttaaaaagct ttgtagaagg tctcctaatt aaggtctcat
     4321 caagaaatat gtttttttaa taggtgaagt tcctgagcaa tatgatacaa atggatgttt
     4381 gcacctttta atgagattgg atagatcttt gtcttttcag tttctaatcc attttgatag
     4441 ctagcagcat cgcacaaaac cactcttttt ttcgcacata gacattcatc aggcaattct
     4501 tctaatgaac gaaataaaat aggatttatt tcagcgatgc cttcttccgc atacaaatga
     4561 aattgggttc gataaaaaaa ttgtttgact gaactttctg aaacaacaaa aatttctatt
     4621 tcctgtctcc ccgcttgttc actgttgatc aaagaaagac ttttgacgaa ttttcgaatg
     4681 aaattttcat tccatcttag ttggatttga tttttctttt tccactcttc caatatttct
     4741 acaatttttc cgtacagaag ttgttctttt tctgatagaa gatagatcct ttccggtaaa
     4801 aatacttgtc catctgccca caacgtacgg acaaattgta aaaaggattg cttgaaaata
     4861 aaattttctg catcgtcgat ttcagcgata ctaataatct gtaaaatcaa ttcattcagt
     4921 gataggtcat tgagaacaaa atttttataa acaacttcga aatcttttct taacagctcc
     4981 atgttctcat tggtataatt tctactgttg aacaaagaaa aaatataata gacatcttct
     5041 tcttgtaagt ctagaattcc tttttgctct tccaatcctt gacagactaa ttgaaataac
     5101 ggcttcgttt ctgcgctttt cttatcttct tctttcagtc caaggggaaa tgcctttctt
     5161 ccgattgcga tctctattcc tcgtatgata aaggactttt catcttccga atatcttctt
     5221 ttaaaaaaat gagtctctac ataatcaatc aactcgttac aagcttcttc tacatacgga
     5281 tttttatttt catagccttc ccaattgaca taacgtacta aagctagcaa gatgttccgc
     5341 gagtcaaatt ctgggataat gatttcttta tttttcaaat aatcattttc tttaaaaaaa
     5401 tccaatacga tttttttgat tgtatatact ttgctcaaag aaataaattc aagatcagcc
     5461 agttctgtac tcgtgaatat accattaaaa taataactta aaaactttaa aaaataagat
     5521 tgaccgtaca gaaactgtgt gagctcagaa agagcaatac catgtttgaa tacgagttta
     5581 tacttatgct tctctcttac ttgttctttt atacaatatt ctagacactc ggctagactt
     5641 tctagatcgt gaaggatcgt tggtgtagtc acccttagag aaatagctgt ttgttcgatg
     5701 tctatttctt ggaattcaaa taattgttca cacagataca cttttctaaa tatatttcgc
     5761 tcgatgtaat tttccagcat tcctgataca cgcctccagt ttttcttatg attttattta
     5821 atcatatctg taaatcgtat ttttgtttcc tttttattta gaaaagccct ttcaaaaacc
     5881 aataaaaaaa gaaactggga caaaagtcaa acaagctcac aatttcagcc tcattgtagg
     5941 tagctttgag ccgtttggac ttttacccca gcctctttca agaatggtta ttcaaagatg
     6001 tcagcgtcta gttctttgac tacatctttc aacaattgga taccagctgc gctgttacca
     6061 aatggatcta gtgccgggct gaagacacct atgccgtaac gatttggcac agctgccatc
     6121 aacccgccgc ctacaccgct ttttgcaggt acaccaacat ggacggaaaa ctctcctgat
     6181 tcatcataca aaccagcagt tgtcataatt gatttgacga ttgtagcact ttcttcagta
     6241 atgatttgtt tgccattcca aggagcaatt cctttatttg ccagcacagc agctaatttt
     6301 gctatgcctg tagcggtgac attgagggag cattgtctga aataagcatc gagtatctcc
     6361 ggtacgtctc cttcgatcat ttgattacct ttcatatagt aagcaagaga gcgattgata
     6421 tcacctgttc ttgattcaga atgatagatt tcttcattca atgtgatatt aggatcatca
     6481 cagatctctt tcataaatga caagattcgc tcaaattttt catctgcatc tttaccggca
     6541 atcaacgagg ttgttgcaat cgctcctgca ttaacaaaag gattcattgg atgtttgcga
     6601 tgattgattt ccatattcat gatagaattg aaggcaaaac cggttggttc cgtgttgatt
     6661 gtttgaaaga ctttttctat tccgttgtct ttgattgcta ataacaatac gggaactttt
     6721 gacatgcttt cgatggcaaa gcgcacatcg gcgtctcctc cctcaatcag ttgttttgta
     6781 tccaaatcat aaatcgccat acctaattgt ttgggatcca cttcagctaa tgctggaatg
     6841 taatccgcca cttgtcctgt ttgataatag tggaaatttt ttccaatcaa ttgttctaat
     6901 tcttttactt ccatttgaat aaccagcttt cattttttat ttttctgcat gctggaattt
     6961 atgacggcat gcgtagatcg taaatggaat aactaagacg ataagataag aaacaatcag
     7021 aatcgtttca taaatcccat gctgtccagc tgcgatacta gcaggtggaa caaatgagat
     7081 acaaaaagca aaaagtgaag tcaaaagacc taaacaagca acgattactt ttacaacttt
     7141 tccgcctgga acctgatagg ctctttcaag actgtcatct ttaaaaacca gcttcaaata
     7201 gcccgcgaaa aacataaagt acaacatcag gtagattaca acagtcaatg acatcgctgc
     7261 aagaaatgaa agattattcc ccccgccgct taatgtaaag acggctgccc agattgttgc
     7321 aacgatccca tttgcgatca tcaaatgagc aggaacaccg tgtgtgtttt cttttgcaaa
     7381 atattcagga agtaatcctt cttctgctgc tacgtgaagt gcttttgaag gagaggttac
     7441 ccaagaagca atttctccga tgacacctaa acaaacaaga atcgcaagaa ttttcgcaat
     7501 ccatgcaaga tttggatcaa tgtgtcttaa taggaattct aacgattgaa tcacccctgt
     7561 attaagagac aaaccatgtt gaggtactgt agtagcgacg gtcaatccgc caaaagtatc
     7621 taaaatgatt gccagaataa ccaatacaaa cattgctaat ggataatcct ttttagggtt
     7681 ttccatttca tttgcatgac tggcagatgt ttctacgccc atatatgcta aaataaagga
     7741 aacaaaaaca accagcgtag gcaatttcga aaaatcagga atcaatgttt tcatagacag
     7801 tatcgtttgt agtggagcgc ctgaagcgac ataatggatc cctaagccaa acaagactaa
     7861 tgctggcaac aagataccag ctacaaaacc gatccgagca atcaatgaag tgtatttggt
     7921 tccttttaac tgtgagagta aaatagccca gaaaataatc aataggattg ccaatttcag
     7981 atatttattg ttttgtatag caccaatacc cgttgcatag gataatgcac cagttaagaa
     8041 atacagcatg gtcaagtagc caactgttat ctcaaaccat tggaaaaaga ttgcggcaaa
     8101 accgaatcgt tttcccaatg tgcgactgac ccaagtatag acaccgcctt tttcccatcc
     8161 tttcaccgtt gccatctctg ctgcgcacaa tgccacaggg ataaaccata gcaaaccgcc
     8221 taataacaag aaaaatacta agctgaagcc tgaggtcgcg aatgtgggat attcatataa
     8281 tgaaacgacc attgagatgg tcatagccaa aaagccaaag aacgttaatt tttttgcagg
     8341 aattttcgtt ttgctttgtt gcatgaatct tcctcttttc tatttgaatt tgtttcaatt
     8401 gagtttgttg ccaaagaaga aaaagtccaa tccttcttct tgttcttctt ttgtagtagt
     8461 gatcgttgta aagtgcatcg ccgattttaa aatcactgtt gtcagcacat aggcatacac
     8521 ggcagtaatg atgacaccaa tcacttgaat ccctaagaag taccagctgc ttggattcgt
     8581 tagtaaatgg ctgttagatg caaagatccc tatcagaatc gtacctgtga atccgcccat
     8641 gccatgaaca ccccacacat caagtgcatc atcccattgt ttctttttac gaaaatcaac
     8701 agcagcatga cagacgatac ccgcaatgat cccaatcagc atggcacttt ttgcttctac
     8761 ataaccggca caaggcgtaa tggctgccaa tccagcaacg gatcctgtta gtacgtcgac
     8821 aaaatcaaag ccatttcctt ttacttttgc aatgatcagc caagtaatca tcgcaaatgc
     8881 aagagcgatg aatgttgaac caaatgcatt gactgcttgt tcatctgctc gaagtgcacc
     8941 accggaatta aaaccgaacc agccaaacca aagcagtcct gtaccgatag cagctgccat
     9001 aagactgcta tgctgacttc tttctcctaa aggaatcttt ctttgaccaa gtgtgaggat
     9061 acatgctaaa gagccgaatc cagctgtcgt gtggatgact gctccaccag cgaaatctct
     9121 aaatcccatc tgatccaaaa atccgccacc ccatatccag tggcaaacag ggaaataaat
     9181 cagcagattc caaaaaataa caagtataat atagcctttt aaattcaatc tccctgcaaa
     9241 agctcctgtc atcaaaggaa cggtaatcac gataaacatc agttggtaaa gaaagaacat
     9301 taaaaatgga atcgttgcac catgaaacat gttggggaag cttgtaacat gtctcatcaa
     9361 aaagaaatct gccgggtttc caataacacc gccgatatcc cgtccaaagg ccaatccgaa
     9421 tccaccaaat acccacataa tcgttgttat cccaacggag agaaaagatt gagacatgat
     9481 cgttagtata ttattcttcc gtgctaatcc gccgtaaaaa aaagctagac caggagtcat
     9541 caggcaaacc attgctgtac ataaaatcat gaatgctgta tctcctgtat tgataggtaa
     9601 tgtattcatt tttttcgcct cctttctacg attacgagta taaaagaagg tgtagttgtt
     9661 tttttcttct gtttttttga attaatactt gcatttataa aagacagaca attaataaaa
     9721 gcttgatgtg ttgtgctctt tttcgttttt cacacaaata aagacaaaaa ataaacggtc
     9781 ttctttttat agaaaaccgt ttattttcgt ctttttttat ttttaaagat attttttcat
     9841 aaggaataaa gaatacttca tataaaatat ataaaaaaga accgcagcga gtgtcgctac
     9901 ggttctcaaa tttgttcttc atttcaacat aagtttttct tcaatttgct tataatttac
     9961 gtgcaaagaa tgaaacgact aatactaaaa tgattgcacc gatcaatgat ggaatgatag
    10021 ccattcctgc aattgttggt ccccaagttc ctagtaaagc ttgtccaatt gctgaaccta
    10081 ctaaaccagc aatgatgttt gcgatgatcc ccattgatga tcctttattc gtgattgctc
    10141 ctgcaattgc tccaatgata ccgccgacga ttaatgccca aataaatccc attttaaatt
    10201 cctcctaact gacttcttac ttcttattgt acacgtggtt cgttattttc tttgactttt
    10261 tgtgcacctt tgcctacagc agattttgct ttatctgttt ggcgtgaagc aaattgtcct
    10321 gtggattttg ctgcttctgt tactttgtct tgtactgttt cttgatcttt ttggtattct
    10381 tcttgtgttt tgatgtctgc aacattcaca ttcacttcga taacatctaa atgtgtcata
    10441 ttgtttactt cagtactgat caattctttg atttggttgt aaatatcttc gatgtcttta
    10501 ccgtattcta cgatcacatc tagatccaca gctacttgtt tcttacctac ttctgtattg
    10561 atacctgctg ttacgttatc tgtgttgact aatttttcag ccatatttga gaaaaatccg
    10621 ccatctaatg tcaatagacc atctacatct ttcaatgcat aaccgatgat tttttggatg
    10681 actttgtctt cataagtcaa ttcaccattc atcatgcggt tttctccttt ttcatttgcc
    10741 aaaactgatc catttgttgt attcatgttt ttgttttcca tctttgtttc ctcctatgat
    10801 ttaaaaaatt gatcaactat tcctgactgt ttcaaataaa aaccgattcc tgcgccaatt
    10861 gctgtcatga ttatgatcaa caatgtcttg aaaaatccaa tcgttaacca taaaacagct
    10921 agcagtagtc caagaccccc aaagataata gggagcttgt atgctgaaaa tagttctttc
    10981 attatttggc cctcctattc cactcgagag tgcttgtagt ctcttgtatc ttcttgttct
    11041 tcataaccgc tgtatgtgac tgccactttg accgtttctt ttgttccgag tactttagtt
    11101 acttcttctt cgatatcttg catcaataca cctgttttcc ctactaatga agaagttcgt
    11161 ttcaagtctc cgttgacatg gatatggatt cgatttttcg ttgaacgtat ccgtacttta
    11221 ggtgaatgaa tgaactcttc ttcgtgtaaa tgggaacgta ccaacccttc gatcgctttt
    11281 ttgtcgattg ttagtttgcc atcttcccgt ttcataacga aagttcctct tgactttgga
    11341 tagaaaagaa cgactagcaa gatgataata gcaagaacgg caaatactac agctgcccag
    11401 aaaatatatt gttgcatgta gtaattcgtt aaagtaaaga agcgaaatcg ttccaattgt
    11461 tcaggcataa tatacggcgc attgatcaac gcaatcgcta gaaatactga aagtagcagt
    11521 aaactcatga taacaccgat tatttttaac cccttattca ttttccacct cctataaact
    11581 tatttttacc ctgtaaataa ctctaccgac aatcacagta tagatagcga acagttagca
    11641 tagcatcagt aaataaagcg ctaccaaaca ttgctggatc ataacggcgt tttattgcca
    11701 gtctgatact gaaataacaa ttgcttactt attctctatt tacttttgat tgctggctat
    11761 acttaaagga tattattcaa gattaaaaat tgcaaagatt atgctcagtt attattacaa
    11821 gattctattt a
//
LOCUS       DADVXU010000082        11814 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.086, whole genome shotgun sequence.
ACCESSION   DADVXU010000082 DADVXU010000000
VERSION     DADVXU010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11814
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.086"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(144..521)
                     /locus_tag="KB953_002138"
     CDS             complement(144..521)
                     /locus_tag="KB953_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867103.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(821..1702)
                     /gene="galU"
                     /locus_tag="KB953_002139"
     CDS             complement(821..1702)
                     /gene="galU"
                     /locus_tag="KB953_002139"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBB6867104.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(1745..2764)
                     /locus_tag="KB953_002140"
     CDS             complement(1745..2764)
                     /locus_tag="KB953_002140"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBB6867105.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(3397..4401)
                     /locus_tag="KB953_002141"
     CDS             complement(3397..4401)
                     /locus_tag="KB953_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBB6867106.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(4414..5463)
                     /locus_tag="KB953_002142"
     CDS             complement(4414..5463)
                     /locus_tag="KB953_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBB6867107.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(5480..6301)
                     /locus_tag="KB953_002143"
     CDS             complement(5480..6301)
                     /locus_tag="KB953_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBB6867108.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(6288..7067)
                     /locus_tag="KB953_002144"
     CDS             complement(6288..7067)
                     /locus_tag="KB953_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBB6867109.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(7084..7554)
                     /locus_tag="KB953_002145"
     CDS             complement(7084..7554)
                     /locus_tag="KB953_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBB6867110.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(7566..7985)
                     /locus_tag="KB953_002146"
     CDS             complement(7566..7985)
                     /locus_tag="KB953_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HBB6867111.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(8066..10843)
                     /locus_tag="KB953_002147"
     CDS             complement(8066..10843)
                     /locus_tag="KB953_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBB6867112.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            11034..11258
                     /locus_tag="KB953_002148"
     CDS             11034..11258
                     /locus_tag="KB953_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867113.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT         3423 a         2485 c         1912 g         3994 t
ORIGIN      
        1 ggcttatccc gatacaacaa gcattccttt aaaaaagatt cttttatata agtgaaaaaa
       61 gcttttttcc accaattaaa taaatctctc atctcaatag tttatccatt attttatctt
      121 tttttgcgta tacctactaa atattaccgt cttcttctcg ttcttctact ttgttcgaga
      181 gaatctgtac tagaaaaagt agttgtatgt ctaccttttc tgcctatccg agactttctt
      241 ttcatatatg ggatataaat gattagatac agtacagcaa aaaataaagc aaatacgcaa
      301 gcttcccaac tacctaacgc caattcaacc atcaaggtag caaccccaac ggatgctgca
      361 taccagataa aaatcagaaa cgtttctttt tttctgacca tgcgaaacat gataaataga
      421 ctaataagca atagtggcaa cacaaatatc caattgcttc cttccatcag ccacttgagt
      481 atatcaagca gcgactgtgc aacagcgact aattttccca tagtgaacac acaccttttt
      541 ttataattaa aaaattcgcc tttttattct actatgaatc ttctggcttg gctttcattt
      601 ctcttatgta atcattttct tttatacaac ttaaaaaaaa attaaaccat tagcataata
      661 ttttctgaat ctattcacga aagtcttttt ctatctttcg ttttccacga ctcaactgat
      721 ctagagtcaa aaaactaaaa cgacagttgc gcaaatgacg cacaagctgc cgctttaata
      781 acaaatagcc tttcattctc agcaggccac ttatttccag ttaactttct gttcttagct
      841 ttttactcaa ctggataata tatgcactta atgaagtccc aatttccgaa tgttgaagtc
      901 catattcaat atttgtcttc atatatccta acttatcgcc tacgtcgtat ctttttcctt
      961 taaactctct cgcatagacc gcctctgtct ggttaagagt ctgaagtgca tccgttagct
     1021 gtatctcgtt tcctgctcct ggttcttgag tttctaatat ctcaaatatt ttaggagtca
     1081 gtagataacg tccgataatg gctaaattac ttggtgcttt tcccatttca ggtttttcta
     1141 caaacccttt tactctgcaa cgaccctttt cgctatctgt tttaggatca ataattccgt
     1201 atttagatgt ttcattttct ggtacacgca tgacggcaac agcggaagct tgtgtttctt
     1261 cataatccat catcaattgt tgagtcaatg ggatatcatc tttcatcaaa tcatccccca
     1321 gcatgacgac aaacggttca tttcctataa atgcttttgc ttgaagcacg gcatccccca
     1381 gtcctcttgg atgagattga cggatgaagt gaagattgac ttgagtcgtt tcttcaacta
     1441 aagcgagtaa ttctgttttt tccttttccc gcaaattgtt ttccaattcg atatttgcat
     1501 caaagtgatc ttcaatgggt cttttttctt ttcccgttac gatcaagatg tcttcgattc
     1561 ccgaatccaa tgcttcttcc acaataaact ggatcgttgg tttatccacg attggaagca
     1621 tttcttttgc catggctttt gttgccggca aaaaacgtgt tcctagccca gcggcaggaa
     1681 tgactgcttt tttgaccttc acttgctgtt cctcctaaac gacgattaac tattgatcat
     1741 ctatttaacg aagagaaaat tcattctcca tttttccgtc acgtaacatg atttcttttg
     1801 ctgctttttt gatatcttct ccattataca aaacattata gattgtttga gtaattggca
     1861 tctcaacacc tagctgttga gctagctcta ctgctgcttt tgtcgttgcc acaccctcaa
     1921 cgaccattcc catgttttct agaacttctt ccagcttatg tccctgtcct aacaagttac
     1981 cggcacgcca attgcgagaa tgaacacttg tacaagtaac gatcaagtcc cccacaccac
     2041 tcaaaccgat aaatgtcaga ggattggcac ccatcgcgac accaagacgg ctgatttctg
     2101 ccaatcctcg agtcatgatt gctgcttttg catcatctcc gaatcctaat ccatggatag
     2161 cgccggcacc aattgcaatg atatttttca gtgctgcacc agtttctacg ccaatcacat
     2221 cattatttgt ataaatcctg aaataatcat tcatgaacag tttttgtaca taggctgctg
     2281 ctttttgatc aatacttgca gctgtaattg ttgtgatatc atgtactgcc acttcctcag
     2341 catggctagg accagataat acaacaattg cctgtctttt ttcttctgga atttcttcag
     2401 ccaaaacttc tgagattcgt ttatgcgtat cttgttctag tcctttactt gcatgtataa
     2461 tgattggctt agtattcatt ttaccaataa gttcctgtgc gactgaacga atcgcttttg
     2521 ttggaacaac aaataaaata gcatctgcgt tatccactgc ttctgccaat tctttatatc
     2581 cgataatcga ttctgggatc gtcaaatcag gtaaaaaatg ctgatttgta tgatacttat
     2641 tgatttcatc aatctgttca ggtacatttc cccaaatacg gacatcatgc ccgttttctg
     2701 ccaatgtttg tgctaaagca gtgccccaag atccaggacc aagaactgcg attttttgtt
     2761 tcatggatat tcctccaaat aaataattga taatttccat tatagtatac atttcttaaa
     2821 gttgggacta atttccattg tttttaagtg tttgtttact tcttttatcg tttttacttg
     2881 aaataatcaa tgatatgtta caatagtcta cattttattt ttacactcgt tatgctttgc
     2941 ttgttcctaa gaggaagcag ttttgacaat gttactacca gcattactat taggaggtta
     3001 ggaaacttct cctagtgagt attgatttaa aatacataag agacagattg aacatttcta
     3061 aatctgtcag gtcctgcaac ggacagattg tctctgttga cggttgacat aggaactttt
     3121 gttttagtca acattctgga tacaaaacgc tatgtaaact ggcgcaactc gttgtacttg
     3181 gacttttact acggatccat gcaacaacta aatagtacta ttgcataaca aaaaatggtt
     3241 ggcacccaca tgtcccatta aacagattat aagttgaact gtagataatg caaaaaatgt
     3301 gttttataac gtaattgatg ccaatatttt aaaaaaataa agggagcagt tatcgctgct
     3361 ctctttattt tttatattct ttttatccag tctatcctaa taatcaaact gacgatagta
     3421 gcggcgtatc tctaatggat ggcagttcaa ctgctccaca tgagcatcga tgcgttgtgt
     3481 cactacatgc atcaataaga aagacaaaac acgtccgcga tatttttcac tgataccttc
     3541 tactggatag tcttttgtgt ctatcaatgt ataattgctg caaatttttg gcaaaagttt
     3601 agccacacgt tcagataaca ctctctgttc atcttcgcct aaaaataatg tgactggggt
     3661 tgtttcttct acgatctcta acgtcccgtg aagaaactcg gcagcatgga tagatttcga
     3721 tggaagccag ctttgttctt cccagtaaca catggcatat gaataaacag ctccccattg
     3781 attccctgct ccaataaaat aatgcatact atcacgtcga tgtttttctg caaatgataa
     3841 gccaaatgca tctgcttttt tctctgcttc cactaaccct gttggtaaat aacgttcgag
     3901 ctgttcatag tattctgaat aagcttcgaa ttctccattc aaatacatca atcgatctgc
     3961 tgccataaaa aatttgattt gttctgttcc aggagctgga taagtgacca caaaatcgca
     4021 tagctgagac aacttactgt tcgctttatc gataaatcca atcagtgtcg ctcctacttt
     4081 cttgatttgt tctactgctt tcactacttc ttgagttgta ccggtaacag aggacaaaat
     4141 aacgatagaa tcttttgtta atcttttgtt tccagtagtg taatattctg cagcatgctg
     4201 tacaaagaca ggcaagttgc ttttcccatt catataagtg acagcttgca tcgaggaagc
     4261 atacgttcca ccgattccta gataatagac agcgtcaaat ttttccgcat aaagtttatc
     4321 gatgatttct tctacttgcg gacgtaaagc tagtgcgcct tttattgctt tgatttgttc
     4381 ttcttcattg aatttcagca tgatttccac ctcttataat ttacttttca agactgtatc
     4441 aaagtcttca aaaatctttt tgttcaagtc aataccttta gctactgcgg ttttataagc
     4501 gaatagttga aacggaacga caagcgctaa tacagacaga tattcatcac attctattgc
     4561 taatccaagt gttttctcgt ttcttgcttt ttttgtggta atggtgagag tctctccaac
     4621 gtaccgactc atatatcttt gcaacttttg cgaacgttcc ttatttacac tttccccttc
     4681 aataaagaat agcaaatgcg aagcatctgc ctccaggtat gggccgtgca tataagcttc
     4741 taactcaaat ccttgtgacg gaacgcggat tgtttcagtc aatttcgtct ctgcttcttt
     4801 agctgttccc cagtttggac catatccaat ggtgacgaaa cgctgtgcta aacgaaataa
     4861 actttgatat ccatcaaaaa attcttccgt tttttgaata atcgctggta aatggttgat
     4921 tattttgcgc aactgttgca tgtagtcttg ttctattttt ttgctgattt tatttttact
     4981 tattccaatt cctatcccta agagtagtaa ctgtaaaaca gtcgcagaat agccttttgt
     5041 aacgaagcct actgattcta ccccggcacc taaatcaata atttgtgcag cttctaaagc
     5101 aagtggactt tgcacgtcat ttgtgatagc aacaacaggt aacccttgtt tttgtatcat
     5161 ttttactgct tcaacagtag aagcactttt cccgctttgt gacacagcaa tcacagtatc
     5221 gattgaagga gaaagttttc cataattgaa aaaataaaaa ggttcttgaa tatcgacagt
     5281 aagatcacca taagattcca atgctggctt cgctgctaaa caagcgttat aagatgaacc
     5341 cgttgctaag atcagtaaat tcactgtttt tttcatatgt tctcttgtcg aaaaatcatt
     5401 ctttttcaag atcgtactta gtacttcctt ttcttcatag atataatctt gcatcgtaaa
     5461 cattttctct gctccttttt taaaaaatcc ctatccacga accaaaaatt ccaaagaaag
     5521 ctagtccgat caagatcgtt aatggtttca cttctttctt caatagataa aacactatgc
     5581 caaatgctcc aagacataag atatttggaa cgatatcatc aaaaatactt tgaacagtca
     5641 cagcattttc tcctgaaccg atttttaaag gaatattgat ggtgatcatc gtggcagtca
     5701 ttgctccaac gaccatcagt ccaataatag acgcgcctaa tgtcaaactt tccatcatac
     5761 cgtttgcctg gatctttttc aaaaaacccg ttcctaagcg atatccccaa cctgttgcta
     5821 gatagcgaaa cagaatgtgc ggaatattga aaatcaaaat gaacaagata gggcctaaaa
     5881 tatttccttg catggctaaa gaggtaccca cacctgtggc aatcaatcgc aaagttcccc
     5941 aaaagaaaga atctcctaac cctgcaagag gtcccatcaa agatgttttg atactatcga
     6001 tcgcagtcac atcaaattca ggatcatcct tattttcttc ctccattgca gccgtgattc
     6061 ctaaaatcag tgtaacgata tgtggggtcg tgttaaaaaa ctctagatgc cgtttcaaag
     6121 ctgctgccat ttcttctttc gactgatata actttttcaa aatcggaatc attgcataaa
     6181 cataagccat attcatctga cgttcataat tccaagaaaa ctccatttga aaagaacgcc
     6241 aaaatacgtg atttagctct tttttcgtta ttttgctgtt tttgatctca gaattcttca
     6301 tcttcttcta cctcccctgc agttgtttga agtggtagtt gacggacatt cataatattg
     6361 actaccacaa ctgctaaaat cgcaccaaaa atagctactc cggtcacagg aatctctaag
     6421 taagaagcta tagcaaaacc taagaaaaaa tatggtgcaa cttgtttgtt gatcagcaaa
     6481 cgagcaagca tcgcaaaacc aagagctgga atcaaccctg tagctactga caaccccttt
     6541 tgaacgaagt caggaatcat atttaataga tttccgatcg tattgcttcc caccatataa
     6601 gaaaccatta ccacaacacc aagcatcaag gaaagaccga taccggcaag caagtgcata
     6661 tgctcgactc ctttataatt tccttcttct gcataattgt ctgctttatg gttcataatt
     6721 ggaatcaaaa gccctaagta aatatttttc aatatcaatg tcaacgtagc aatcggcaaa
     6781 cctagtaaaa gcgcggtttc tgtaccggct ccagctgtaa tagcaaaagc cgtccctaaa
     6841 atcccaccgg tcacaacatc aggtggaatc gaagcaccta ctgagaacga tccgataaat
     6901 gctaactcta aagttgcgcc cataatgatc ccagttttta catcacctaa cacgattcct
     6961 gtaaacagcc ccgtcacaat cggacgagaa agcaacgatg tccctagtgc atattctgat
     7021 tgtgcgacaa atgcaacaat tcctaataag actgcttgta caatcattga ggttcttcct
     7081 ttcttacaaa acttctctta catccacttt tttatctgct ggtacttgac ggatttctac
     7141 ttctatttgt ttgtcaatta atcgtttcaa gcgttcctcc tcttctggta aaatatttac
     7201 cgcttttccg atgctgcgag taccaggttt tgccttgatc cctcctaagt tgatggtttt
     7261 gatttctgga tacgcttctg ccagcttata tgcatcctcg atcgattcca ctacgatgaa
     7321 taacttatat ttatctgtca ccccgctttt caacgcggca atcgagtcat caattgattt
     7381 gatcactagt ttgactcctt gtggcttggc taatttgatc gttgtctttc gtatctcatt
     7441 cgtaggtaca tcatcattag caatcaaaat acagtctgcg cctattgttt gtgtccatga
     7501 aaaagctact tgtccgtgta ataaccgatg gtcgactctt gtcaatacaa tcatttttct
     7561 tcctcctaaa attcgtcttc ttctattttc tgattgagcc gctcgttaca aaactggatc
     7621 attttttttg aatcagttag cacctgctgg atcaaaacag ttgtctctac ttcagcgtcc
     7681 aaccgagata ctagctcaat caatagtggc aaattcaatc cggcaacgag atagacatga
     7741 tcccgattga tatattgtaa gaattcgttg ttgacacttc cgccaaaaag atccgtcaga
     7801 acaatgattt ctttatcttt gtacctcata aaaagctgcc ttactgtttc cgctaaatca
     7861 aaattctcat cagtatagca atcgaataca gtaatagaat gatttttccc atagatcagc
     7921 tctaatgaat ctaatatccc tgaagcaaat tttccatggg aagctaagat aatttctcta
     7981 tccacttttt catctcctct tttcacttac acatactaaa aaagcaagtt gcatgccaaa
     8041 attagtggca acaacttgct tatgttcaaa attcttcttc tatcgagaga aactctgtat
     8101 cttcactcaa aatatcatag atataggcaa tctcagaatt ggggatcatg acactataat
     8161 gctcttcaat gacactaaac gcagatttaa tagcagataa cccttctttt tgacactgct
     8221 tccatttctc taatccttga taggtttcga ttggcatttg tcggatcaat cgttcaatga
     8281 gacagctcac atggacatac aacgctaatt ttttgggatt gctgatgctt cgattcaact
     8341 gcacctctaa ttctctcata aaatgatcga tttctcccat caccttctct gtatctagaa
     8401 tagtcacgga atccagtact ttttccaatg aaaaattacg aatgatgcgc tgattgaata
     8461 tttccttctc tgaagcattc atagtagtgt ccaaccattc agctagtaca gacatctttt
     8521 cccccgagat gaggtcttct aatgaaagat agtcgatatt ggtgatatca ggatctgtgg
     8581 tgccaatcat tcctacgact tcgtatatgg aaaataatgg gtcactgttt ttcttatctt
     8641 ttaattgctg atattcatat gggatgattt tcaactgaca agccgtaggt aagctttttt
     8701 ctagcagttc acttaagtgg accgccgtac cgattccagt gaagcaagta gtaatgagtg
     8761 ctttgggttt gttttcctct ggatagagca gtcgccattc tggctggatc tgtcgaacag
     8821 tctcttctgc tatctcttct aatgatacat ttttttgcag gcattcccct actgtgattg
     8881 ctaaaggtgt cgtcacatta ttcaaaatca aaagcggtac ggataactgt tttttaaaaa
     8941 actgatggat ttctttcaga gatcccatgt ctactaatat gatcaatcca tttgaaacat
     9001 cgctgttttc gcaataatca aggatttctg aagcaatctg ctgtggtgtg acatctagtg
     9061 gcatatcaaa tgattcaaat agatcctctt gtaataaacg atttactaca tttgccatac
     9121 tgctagcagt tgcataaccg tgagcagcaa tcatcgcttt tggcagtcgt ttttcttttg
     9181 accatttcgc tttttttaga tagatcgtca gtaaaatccg atccatactt gtgtcttcaa
     9241 tatctaattt gatcttactt aattccagaa ttcgttggac atagtgataa ctagatggat
     9301 aagactgaga aacttgttgt tctaattctt tgattagttg gataatatca tgttcttccg
     9361 gtaaccattt gatagtgctt cgctgataga gatagtaact caaggcatag acactgtttc
     9421 catcgaattt gatttgatag gcagtttcca tttgtcgaaa ggtctctcgg atagattgtg
     9481 tccaataaac cagcagctcg tgtttttctt gtctactggt ctcaaacagc aaaaaatcaa
     9541 ataattgaat cacttcttgc tttaattttc cttcgcaaga atatagcaga cagttatttt
     9601 tcttgaaatc aattaggatt tgctcaaaac ttttaacgat tcgctgctgt tcgggctgtt
     9661 gcttctccat taattgctct aaggtctggt cttgcgtaat atagattcct tgctgtgctg
     9721 tatttttagt aataggctgc gcaagtagtt ctttttgcaa atgatggatc gtgacatgga
     9781 tttcaggctg ttcacgctgt tcagcatatg cttttgctgc tgaaactttg acgatatttt
     9841 tcagctctcc tatgtttccg gataatttct gacttgttaa aagtgacaaa gctggtccac
     9901 tgactgacaa agggctcccc atttttcttt gttcttcgag aaaaaaagag taaatcagtt
     9961 ctagccgttc attacgacta cgctgattaa gtgcaggtag ttcgattcgg ataggtattc
    10021 ttcgaatgaa agttgttaag aaatgactag taatctcttc agtcgtagca aaaaagagac
    10081 gtgttttgat ccggcgtgac aaattaggtt ctcccatacg ataaatcaca ccttgatcaa
    10141 gataggtgaa cagtttttcc tgaccttctg gtgttaagcg atgtacctcg tctaaaaaca
    10201 agatcccacc atctgctgct tcaaaagccc cttttttatt ttcttctgca ccagtgtacg
    10261 ctcccttaac atgtccaaac aaattgctag tcaaaagttc aggattatct gcatattgtg
    10321 cacaattaaa agtgatgaat ggtgcagaat cttctagcag atcatgaagc agacaatact
    10381 gatagaccaa attaactaaa taacttttac ctgtgccact ttcgcctgta atcaaaagtg
    10441 gtaatccgcc atctggatag taaagagctg ttttgatttg ttcgatactt tctttcaaac
    10501 tggcgtcgta tccgatcata aaagagaata agtcttttgg ttgcgcgaaa aaaggctgtt
    10561 cctctttgag atcatcgata gaaaaataaa aattcgtgcg taacttaaaa aattgttttt
    10621 caaaagcttc tttgtggaaa taaatcactg gtcgagaatt gattttgact agtagttgct
    10681 catcatttaa ctggttcaaa taatgactga ctgtgtttct ttttacccca aaatgatcag
    10741 ctaacctttg tgctgtaaag acctcgttca tatgttctaa atcgaaaaag gctgtctggt
    10801 tctctaagta acataaaatt tccttcttca acaacttatt cacccctcct ccatcataga
    10861 aaacggattc attttgtcaa taaaatcttt aataaagaat tgaaaaatat atcttattat
    10921 agacagcagc ctacttatcc cttataattt ttattctaaa aaacgtacag ttccttccag
    10981 tttttatact ggaatttcaa catatgaaag aataaggagg ctatgactat tcaatgaaat
    11041 cggataaata taaaaacagg aaacatactt cctggaaaac ttttcgagaa atcatcggat
    11101 cttatctcgc tgtagttgta tttttgcctt tgctttatat ggctatgaca gaacaaactc
    11161 gttttattgg cgtagatacg caatatatag ccgaaacagg atttttattc atctttatct
    11221 tgatttttgc gttctataaa tccttccata caaaataaga ttcacagatt tcaactttcc
    11281 tgtctgtttt tagctattac aaatccttat atgtggtata acaacaattt attggcataa
    11341 tgaggaaaaa cgagttatga tgctttcaga gagtaaagag gtactgttgt caccatcctt
    11401 tttattgatt acgtacacca ctcttagttt tttcccattg attacgaaaa caaggtagaa
    11461 acattgatgt tcctaccttg ttttcttttt ttattttcag atcaaaatct caagtggctt
    11521 ttgacttgat gtcgggaggt tgtcttcgtt ccatcaaacc tgatagaatc tgcgccaaac
    11581 catagttttc tccactatta tttattcctc taagatagta cccacaataa acgaataaat
    11641 atttttctca ttttcatact ccttctttga aaatcaatta gatttctctc aacccacttt
    11701 tttacaaaaa ttacgtagca aaaaagattg caagaacaaa ttaaaaaaga agtaaataga
    11761 gtttcatcta taaacattta cttgatatat tttttatgca gaattcattt atag
//
LOCUS       DADVXU010000083        11639 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.087, whole genome shotgun sequence.
ACCESSION   DADVXU010000083 DADVXU010000000
VERSION     DADVXU010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.087"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            374..643
                     /gene="rpsN"
                     /locus_tag="KB953_002149"
     CDS             374..643
                     /gene="rpsN"
                     /locus_tag="KB953_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBB6867114.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            658..837
                     /gene="rpmF"
                     /locus_tag="KB953_002150"
     CDS             658..837
                     /gene="rpmF"
                     /locus_tag="KB953_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBB6867115.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            869..1246
                     /locus_tag="KB953_002151"
     CDS             869..1246
                     /locus_tag="KB953_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867116.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1267..1416
                     /gene="rpmG"
                     /locus_tag="KB953_002152"
     CDS             1267..1416
                     /gene="rpmG"
                     /locus_tag="KB953_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBB6867117.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1440..2423
                     /locus_tag="KB953_002153"
     CDS             1440..2423
                     /locus_tag="KB953_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBB6867118.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2453..2575
                     /locus_tag="KB953_002154"
     CDS             2453..2575
                     /locus_tag="KB953_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HBB6867119.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2593..4116
                     /locus_tag="KB953_002155"
     CDS             2593..4116
                     /locus_tag="KB953_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HBB6867120.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            4140..4379
                     /locus_tag="KB953_002156"
     CDS             4140..4379
                     /locus_tag="KB953_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6867121.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4572..5033)
                     /locus_tag="KB953_002157"
     CDS             complement(4572..5033)
                     /locus_tag="KB953_002157"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBB6867122.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5228..5446)
                     /locus_tag="KB953_002158"
     CDS             complement(5228..5446)
                     /locus_tag="KB953_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6867123.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5564..7180
                     /locus_tag="KB953_002159"
     CDS             5564..7180
                     /locus_tag="KB953_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBB6867124.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKKIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7421..8761)
                     /locus_tag="KB953_002160"
                     /pseudo
     CDS             complement(7421..8761)
                     /locus_tag="KB953_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9037..9192)
                     /locus_tag="KB953_002161"
     CDS             complement(9037..9192)
                     /locus_tag="KB953_002161"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HBB6867125.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(9488..9820)
                     /locus_tag="KB953_002162"
     CDS             complement(9488..9820)
                     /locus_tag="KB953_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBB6867126.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(10334..10525)
                     /locus_tag="KB953_002163"
     CDS             complement(10334..10525)
                     /locus_tag="KB953_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6867127.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(10619..11005)
                     /locus_tag="KB953_002164"
     CDS             complement(10619..11005)
                     /locus_tag="KB953_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBB6867128.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
BASE COUNT         3882 a         1817 c         2231 g         3709 t
ORIGIN      
        1 ataaggaata atcaaagttt taaaaaattg gcgataaacc agttaaacat aaaagattga
       61 ataaaaagtt atagtttctt tcatattgcc gttttaagta gaggaaaagc tacttaaaac
      121 ggtatttgtg tttgattgga ctaataaaag tgaattctta tttttcagag cctaaactgc
      181 atattctttt aaatgatttg attatctcta tgtaccgata aattcgtttt caaatagtta
      241 tctattcata tgagttttat ttcatgttat ttattgttga caagagcaac tcttgttggt
      301 aaagtatcag tgtcaaaacg taacgattac gatttgaggt gtctaaatca aaataaaagg
      361 agtataaagg tatatggcaa aaaagtcgaa aattgctaaa gcaaagaaac aaatggcgat
      421 gattgaaaag tatgcagata aacgtcagga attaaaagcc gcaggagatc gcaccgctct
      481 cgctaaattg ccaagagatt caaatccgaa tcgtctgagg cttcgtgatc agacggatgg
      541 tcgtccaaga gggtatatgc gaaagttcgg tatgtcccgt attaagttta gagaacttgc
      601 ccatcaaggg ttgattcctg gagtaaaaaa agcaagttgg taaaaggagg aaatatcatg
      661 gcagtaccag caagaaaaac atctaaggcg aagaaacggt taagaagaac tcatcaaact
      721 gtagtaaaac cagaaatttc atttgacgaa gcgaatggtg attatagacg tagccatcat
      781 gtatctttaa aagggtacta taatggaaag aaagtaatta aaggagcttc aaaatagttt
      841 aatatataac ttttaggagg gaatcaaaat ggaaattatt tacccacctt tagttgaaga
      901 aggattaaag tattatcttg aaacgaccca gcaatcgcta gataaatgca ctttttatcg
      961 ttcgatggta gagagaggaa ttattactga aacgggttta ccgacccaac aggcaattga
     1021 aaatggatta gtcaaagatt actatgagga ccaaggctta tcatttgatg attttttaag
     1081 aatctatcca atttttgaag agtatgacga ggagttattt caatgtatcg atggttattg
     1141 ggagatacct gttgatatga aagaaaattt agtttcacaa ttggaatctg gcgagttaac
     1201 ttttgaagat gcacaacaaa ttcaagcata tcttgaagat cgataagtat tgaaggagga
     1261 agatttatgc gtcaaaatat tattttggaa tgtgtcgaaa ctggagaacg tctgtatctc
     1321 accagcaaaa ataaacggaa taaccctgaa cggttagaat taaaaaagta ttcgccaaaa
     1381 ttgcgcagac gggcaatttt taaagaagtg aaatagctaa gtggtaaaaa ggtgacaaaa
     1441 tgggcatacc agttacaatc gtttctgggt ttttaggggc tggaaaaaca acgctaatca
     1501 atcaagcctt gcaagtctca ccctacccca gggaagaaat tattataatt gaaaatgagt
     1561 ttggtgaagt tgggattgat cacaaattat tagttcatag cgaggaacgg gtgttacaac
     1621 taaataatgg atgtatgtgt tgcagtcttc gcagtgattt attagcaata ttgatttctt
     1681 tactggaaac aatagaagaa catcaacaac caatttcaca aatcatcatt gaaactacag
     1741 gtatagcaga tccccaacca atcattcaaa cacttttaac agcgcctcaa ctaagaggaa
     1801 gagtatatat cgatagttta ttgactgtta ttgatagtga cagttttgaa cttattgaaa
     1861 gagaaccaca agcccttaag caacttgtca tggcagatcg catttttgtg tctagaaaac
     1921 aaaacttgaa tttatctgca ctaaaaaaat tacagagaga aatcaaaagt attaaccctt
     1981 tatccgatat tcgaatgttc acacatagtg aaacgattca aaaaaaggat ttttataatt
     2041 tgaaaaaatt taatcatccc ttagtttttg aaagtgattc atcagaaaat cactataatg
     2101 gcaaatacta tcatcaacac catcaagtac atgagtttaa atcaattctc ttaacttcag
     2161 atcaagacat aaaaaaagat tttttactcc aatggattga ctggctgatt ttaaccaacc
     2221 aagggagcct gtatcgtttt aaaggattga ttgctttaca agagcaggat tttatagttg
     2281 ccatgcaaga ggtaaacgaa caggttaatt ttcaatacac cactcgacaa aaagaccaaa
     2341 ccactattat tctaatcgga aaaatgttgg atgaagaaag gatcaaacag agttttgata
     2401 aattactggc ggaaagcaaa taatgataag tagtgattgg gggtaatttc taatggagaa
     2461 aacggatata tctagtgcat atcgacgact taaaagcccg aatattaaaa ctagaaaaag
     2521 ggcattaaaa attatcaaag aacataaacg aaataaaatg aagaaattag cataattaag
     2581 gaaggaaaaa gaatgaagaa aatcagtgta ggacttattg ggatagcagc tctaggatta
     2641 ttgggagctt gctccagcac taatgatgca aaagtatcga atgataaaga tggaaaatta
     2701 gaaattgtaa caacattcta tccaatgtat gatttcacca aaaacatcgt cggcgatgaa
     2761 gcgaacgtgg atttaatggt tccagcggga tcagaacctc atgactatga gccctcagct
     2821 aaggatatgg caaaagctca tgatgctgat gtttttgtat atcataacga aaacatggaa
     2881 tcatgggtac caaaagctaa agaaagttgg aagaaagctg ggcctaatgt agtagaagga
     2941 acaaaagaca tgattttatt gccaggtagt gaagaagaag atcatgatca cggagaagaa
     3001 gatcaccatc atgaactaga tcctcatacg tgggtttcac ccaaaatggc aattaaggaa
     3061 gtttcaaata tcaaggatca gttagttaaa ttgtatccga aaaaagcaaa agtatttgag
     3121 acaaatgctg aaaagtattt gactaaactg aaacggttgg atgctgacta taccactagt
     3181 cttaaagaag cgaaacaaaa aagttttgtg acgcaacacg cagcctttgg atatttagct
     3241 ttggattatg gattgaaaca agttcctatt gctggactaa gtcctgaaga agagccatct
     3301 tctggtcgat tagctgaatt aaaagagtat gttaaaaaaa ataagatcaa ttatatttac
     3361 tttgaaaaga atgctaatga taaaattgca aaaactttgg ccaacgaagc tgggataaaa
     3421 ttggaagtac tgaatcctct tgaaagtctt acaaaagaac aaatggataa tggtgaagat
     3481 tatgtttcag taatggaaga caatctaaaa gcgttagaaa aaacaactat ggttgctggt
     3541 gaagaggtag ttccagaaaa agaagcgaag gatgaaaaga cagttgctaa tgggtacttt
     3601 aaagatgccg atgtgaagga tcgcgaattg agtgattata caggcgaatg gcaatctgtt
     3661 tatccgttgt taaaagatgg aattttggat gaagttttcg attacaaagc aaaaatcaat
     3721 aaagacatga cagctgccga gtacaaagat tattatacta ctggctataa gacagatatt
     3781 gatactatca acattaaaga caacaccatt gattttgtag tcaacggtga acatcatcaa
     3841 tacacataca aatatgttgg atacaaaatt ttgaattatg aaaaaggaaa ccgtggtgtt
     3901 cgttttaatt ttgaaactga tgatgcaggt gctggtcgtt ttaaatatat tcaatttagt
     3961 gatcatggaa ttgcaccaag taaggcagag catttccata ttttctttgg tggagaaagt
     4021 caagaaaaac tctataatga attgcataat tggcctactt tctatccagc ttcattgtct
     4081 aaacatgaaa ttgctcaaga aatgatggct cattaaaatt caatttttgg aggaaataca
     4141 tggtaagaaa agtattattg tctatggatt ccaagaatat tgatgcttat cggcgttatc
     4201 gatttgcaaa gcaaatgaga ttaaatggga gtacattagc tgatattgct gcaactttac
     4261 atgttagtat tgacacagtt catagagtat taaaacggga tcctgattgt tgttttatca
     4321 aggtcaatac tggaagaagt agacgtttag caaaacggca taatagcttg gccaattaat
     4381 tgataatacc agagtagatc atttttccct tatttggatt tgatctgctt tttattatgt
     4441 cttgaattgt caaatcaagc gcaacacttt atcaaagaag acttcataag gtgttctcca
     4501 atctaaacac tttctaggtc tgttattcag tttttcaatc atctgattta tcgttgagtt
     4561 atctaccgta gctatctctt tcgattttgg caagtattct cgaatgagtc tattcgtatt
     4621 ttaatttgtt cattgttgcc aagggcatga ggatctggaa aataaaaggg catattattc
     4681 aatgcttctg taactgcgaa atgattggag aattcttttc ctctatctgg agtgatcgtt
     4741 ttgaccgcct catttggaat actgcttaaa agttcaatca ttttctcgct cacataagcg
     4801 gctttctttc tggggacctt ctttcctgct aaaaggaagc atgattttct gtctgtcaaa
     4861 gtcactaaac aagtaccacc agttttgcca gctacaatat cggctttcca atgaccaaaa
     4921 tcacttctat catctgcgga ttttggacgg ccatggatcg aatgagtaag gcaaatcttt
     4981 ccacgagact cgacatgatt ttttgtatgt cttgtttttc ctcgatggtg caatgaacgg
     5041 atcaggcctt tattgccgtg tgagaggggt tctgtatcga aaagatcatt gcaaattgca
     5101 cgttagatcg tgataaaact aattgaaaag tccatatgtt catgcttcaa acgattcgag
     5161 atttcctctg gcgaccactg aaaatcgaca aataaatgac gaacaattga ccaaactcac
     5221 ggatcattta gaatgtgata acgtccgcat ttttttcttc gataagcata atttttttcc
     5281 gctttggaag gtgaatagtt tttttcttgt ttcttctcag ttcatgtaaa atagtaaacg
     5341 ggctatgctg aatgtcgttg ccaattatac taatagaaaa tccttcttcg tgaagcagaa
     5401 agattctttc tcgttcgagt atagtaagat gacgatactg gctcataatt attttccttc
     5461 taggagtgtt ctcgacaaac ttatactagt tggatatcga ttatgactca tttttatgtg
     5521 ttgcacttaa agtgtaaatt caaggtatag aaagaggttc cctatgaaaa aaatacctat
     5581 tacaatctta actggctatc ttggttcagg aaaaacgata cttctaaatc atattctgta
     5641 cggtgatcac ggaaagaaaa ttgctgtcat tgtaaatgac ataggggaag taaatgttga
     5701 tggggtatta gtcgacaaga aaggttttaa aagaactgga gaaaaacttg tttcgatgcc
     5761 gaatggatgc atttgttgta cattacgcga agatttgatt gttgaactag atcaattagt
     5821 gcaattttct gacttgaatc aaattgttat tgaagcttca ggaatcagca aaccaattcc
     5881 aattattcaa gcaattatat tagctaaaaa ccctttagac attgatttga cccaacgatt
     5941 agagattggt gccattatta tagtagtgga tgtaaaacgt ttttgggata atttcgaaac
     6001 gggtgaaaag attcaggaaa gaaaagctga aggtaatgta gttgaagaag aacaggatat
     6061 tcgtggcttg ttaattgatc aattcgagtg ttgtaacata ttacttttaa ataatagtga
     6121 cttagtgtca accaagacga tttacaaaat taacggtcta attgctaagt tacagtccaa
     6181 tgccaaaata attaggacaa ttaaaggtga agtagactta tcgaaaattc taaatacaaa
     6241 tctttttaat atagatgaga cttcctattc agtgggctgg ttcaaagagt tagagcttgg
     6301 ctatcaaaat catattcctg aaacagaaga atatggtatc tcaagtttta tctatcgaaa
     6361 tcggttactc cttgacgcac aaaaatttgc ccatcttacg gataaaaatt ttcctggcaa
     6421 tattacaaga gcaaaagggt ttctttggtt tgcccaacat agtgattttt cgacgatctt
     6481 tgaaacatcg ggtagctata aaagtattga atcattaaat tattgggtag ctagtttacc
     6541 taaaattgag tgtcaaatga ttatacaaaa aaatcccgaa ttgaaggaag cctggcatcc
     6601 gttgtttggt gaacgcatta ataaactagt ttttattggt atgaagatgg atcaacaatg
     6661 tattaaggaa caacttgagg gttgtttaac tgattctctt aatgcagata atttccgaaa
     6721 aatttcacca tttccatgga aaaagtctaa tatgtttagc cagatatcgt taatgaaatt
     6781 tttaagaaaa gctggtgtca agataggcga tgtcataatt attggcattt tgattattac
     6841 ctcttttatt cccttatttg tattctctat agaaaaggag aatgaaacaa ctgaagatgc
     6901 tatcaaatat gccatcgttc gaattgatgg caaggaaatg gatcgcttcg atttggataa
     6961 gatcaatcat aaactcgtga attatcatcc tgcgaaggga caatataata ttgttgagat
     7021 taaagatgga agaattcgag ttaaagaaga taatagtcct gatcaaatcg cggttagaac
     7081 tggatggatc tcaaaacccg gtcaaacgag tatttgttta ccccataagt tagttattag
     7141 tattgagaaa aaagaaagca cagattacta catttactaa tctggaaata gatgaggagg
     7201 tttttaataa gaaaaagata ttattattaa taatagcagg ggctttatta agtgtgttaa
     7261 gcgcttgtgg acaagaaaaa gaagcggtga caacaggcac aagtatcttt aacgggacct
     7321 gcaatggttt agttaacagt cggttagatt acttattttg gctcttcgtc aatgagtgtt
     7381 ggtgaagaaa ttcaatagaa taatcgagat actcacacgg ctagacagct atcttgtcta
     7441 gccgtgttgt tgttctggct ttagtcagtt gggtagtatt cttagttttc ttaaaatatt
     7501 ttcctcgata taaaaagatt cgttctcggt agttttgaaa gtttctatag ccataggcaa
     7561 ttcgttttag tactttgata tggttattca aacattctat agggccgttg gaatagggaa
     7621 cgcgcaacgc tcgtttgatt tctgtttggt atttcttgaa ggtcgtgaac actggttggt
     7681 agtgctctgg caactccttg tagtcttctt ttaggagcgc gacgaattca ggaacatctt
     7741 gtcttctgat aacggaaaga aaatcctggt agacttcgta gcctcgcttt aaggaatcat
     7801 cataagataa tagccgatcg actatctctg cttctgtcag atgatctcga aatgacggac
     7861 gccagtagcg atgctgacct ttcaattccc acgcattctt ttgaagtagt ttccagtatt
     7921 tctttaagtg cttcgaccga cgatcccctt ttttaaaggt cttcattact tgaattcggt
     7981 ggttggtaaa agcccgacca atgtgttggc taatatgaaa gcgatcggcg acgattcggg
     8041 cattcggaaa acactctttc accaatgtgc gatagggtgt atagaaatct gaaaccacta
     8101 gacgcacttg ttcacggact tttatagggt agcgcaagaa atagtcgcgt aaaggattga
     8161 gttgccgatt ttccacaata tcaatcagtt gatgggtctt gccgtccatc atgacaaagc
     8221 tcatggctcc cgagacattt ttcactgatt tgaattcatc gaaacacacc acttctggta
     8281 acagctggct caaagaaatg gttttaggcc gaaggctgcg aagtattcga atgacagtgg
     8341 tcggagaaat gtgcttcata cgtgcgatca aagacataga aagaggttcg cttaatagct
     8401 caagaatggc ttgtttgact ctgcgagcaa tggagcagcg acgatcacta actgaatctt
     8461 ctgctaaaaa agtctttcca cagtgtttac atttgaagcg ctgtttgtta atccgcagaa
     8521 tggtcttgta gttactgaca tcattcaata agatggatac ttttttccag ccctatttaa
     8581 tgatcgtttg ttcattttga tagtggcgat gaaagcattc ctccggttga taggtcaatg
     8641 tgccatcaaa aacaaaacat gtttcgtcat tgattcgctc tttgcgaaag gcatcttcat
     8701 gaaagataag atttaggtct agaatatcta gtaactcttt ggtaaactct gaataggaca
     8761 tcttgaaaca ctcctttgaa tggttttcgt cgactttatt ctaaaggatt ttaaggtgtc
     8821 tgtttgcgtt tattagaaaa aagaaaaagt attggcaaca gaaggcgtca ccaacactag
     8881 atattataga tcccttattt tcttcgcttc aaattgtcaa ggagtcatca taaattttgt
     8941 atggattctt ttgtttgggt agtagtcttc taaataatct tggattaaca ccttcatctc
     9001 tattttactt ttatatttgt tggagtcgat taattctcat ttgattgatc gatgaaacgc
     9061 ttcaattact gcattgtcaa caggagtccc agtttggctc actgaatgtg tgatgtggtg
     9121 gtcgttcaac agctcttgat aaataaaaga ccgatgctgg ctacctctat cggaatgaat
     9181 taccgtaggc aattctcctt aataaatttg taatggacgg acgacaatct cttttttcat
     9241 gtgtgaatct accatatagt aacgtagaat acgagttgct aagtcaatat aggtggacaa
     9301 atagaggcga ccgtcattac atggaatgta agtaatatcc atcacccaaa cagcatcaat
     9361 ttggttctgt tcaaattatc gattcaacat ttttttacca tttctttgat tgttttttct
     9421 ctaccatgag aagatttgcg tcgatgaaat cctcttgtgc ataaattcat ttcacgcata
     9481 agaacagcta caacacgttt attgatttcg attccttcat cgtaaagttt ttgagtgatc
     9541 cctggagatc cgtatcgatc tttgtgttcg tagaacactc gtcttcctct tttagtcgtt
     9601 tattttcctg ttcaagaatg tagaaatctg ctttatcagg taatcttttt ccttgtccag
     9661 caaaagcatt tttcccgtat cgacgatatt ctgaaaccca tcgggaaatt gttggacgag
     9721 agatattgta ttcttcagca attttcttgg tagttacttt tttctcaagg atatcctgtg
     9781 aaatctttag tttaaattta cgatcatatg tttttctcaa tagagttcat tccttccggt
     9841 aacgtaactg atagtcaaga aaagtgtatc atagcccaac ttagtatcag ggtttgtcaa
     9901 ctaaactgtg gaagttgata gataagagtt tttaacaacc aaaattctct tgactatttt
     9961 tgaaatcaga caaattttga ttggacacat agttgaataa atctaccgtc gttacggaag
    10021 gtaaatcgca tgcttttcaa ttctagagga gaagtcctcg ttgaagtacc atagcccagt
    10081 tctggattcg acctcctgcc cacttcatac gtaaataaag taattttagg agtgagactg
    10141 tgacaaaagt tatttttaag ctaaaaacga attaaaaaac gttgataatt aaaatcattc
    10201 gttttagatt ctatgaatca ttatagaacg ctgtaaaatc gatactttat aattataaag
    10261 aagatagttt caaatttatt gttcgaaatt tgaaactatc tttttactta tgtcacagac
    10321 tcccttagtt gagttattct ttaatttcga atttcgagat agctagatat attgcaagca
    10381 tacattcttg ataacaatct tgatcatatt ttccagttct ccaggattgc ttatgaatca
    10441 aagagctaaa tcttttgaga attgcctcaa cagcttcttc atttccagcc tttgctaaaa
    10501 tgaaatcttc ttttaaagaa ttcataaatc taccttcttc ccatggactt ataatctatt
    10561 tctttgggaa ggaggagaac gagcaaagaa gcatacaaaa ttagttatta tgcagatatt
    10621 aaaccagcat tttttgaatt tttttgtaaa gtcgatgttt taaatttgtc gcaccctgtc
    10681 ttgtaatccc cagaaccttt ccaatttctt tatcagtttt atcaaagata aatttttcga
    10741 taagaaaaat ttgttcaggt tctttcaaca aaggaaggat ttttgctaat tttatatctt
    10801 caacaaggag agtcacattt aaaacattaa tcggttgttt aaattttata gtatctttag
    10861 taaacaaata tggtgggagt tgatcaagtt gttcatgttt ttgaatttct tcttgttttt
    10921 tataataagt attagcggtg ttcaaaatga ccttttcaaa gaatcgaatc aactgggcat
    10981 ttacgcgttc gtcgttatct gacatattgc acacctcctc cattaagatt taaagggaaa
    11041 agaggcaaaa aacacgaaaa gaagctaatg caattatgct gcgctgcgaa taacttctat
    11101 aagctcacat gcattgatag gactgctgat gtttattttc aaaaatgttt ttttagttga
    11161 taatggatag ttatctttat acttagaaaa gaaaattcat atatcttctt aaatatcaaa
    11221 catgtcctta tggctaagaa aatgtcttga gctttttacg tataataaat cttttctaaa
    11281 acctcctttt ggataatatt cttattcgtg tatagattgt attttttatt tcttccccct
    11341 tcataattaa caactagaaa agagttcgaa tagacacgta tttgaacgaa aatttttatt
    11401 ggattcccaa attggaatat ttagaaacga aaaattccta ttatctgtat agtgcttaga
    11461 taataacgga tcataataat tattgtcttt gaactagata tatgtaaata gtattcatca
    11521 tgatgaaaaa tgatgagttc tcggtgataa aatgtacttt ttacggtgga atatacatga
    11581 aagcgataag tggatatttt ataatgaagt aagttagctt attttgacag aggaatagt
//
LOCUS       DADVXU010000084        11618 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.088, whole genome shotgun sequence.
ACCESSION   DADVXU010000084 DADVXU010000000
VERSION     DADVXU010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.088"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(241..726)
                     /locus_tag="KB953_002165"
     CDS             complement(241..726)
                     /locus_tag="KB953_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBB6867129.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    816..1161
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1206..1847
                     /locus_tag="KB953_002166"
     CDS             1206..1847
                     /locus_tag="KB953_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBB6867130.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            1866..2501
                     /locus_tag="KB953_002167"
     CDS             1866..2501
                     /locus_tag="KB953_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBB6867131.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2519..3334
                     /locus_tag="KB953_002168"
     CDS             2519..3334
                     /locus_tag="KB953_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBB6867132.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3653..5089
                     /locus_tag="KB953_002169"
     CDS             3653..5089
                     /locus_tag="KB953_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBB6867133.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKSSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5278..7710
                     /locus_tag="KB953_002170"
     CDS             5278..7710
                     /locus_tag="KB953_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBB6867134.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQLSFVPI
                     DSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQDYELPT
                     VDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEVQPAVG
                     VKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIEAQPNH
                     PDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRAKPHEV
                     KLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAATGVRNI
                     SGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMARAAGI
                     HMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGDMLFLP
                     MGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQPQDELF
                     EEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKVFIEQE
                     ETSETLPDDNDM"
     gene            7940..9007
                     /gene="rlmN"
                     /locus_tag="KB953_002171"
     CDS             7940..9007
                     /gene="rlmN"
                     /locus_tag="KB953_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBB6867135.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(9200..10303)
                     /locus_tag="KB953_002172"
     CDS             complement(9200..10303)
                     /locus_tag="KB953_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBB6867136.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10855..11403
                     /gene="aac(6')"
                     /locus_tag="KB953_002173"
     CDS             10855..11403
                     /gene="aac(6')"
                     /locus_tag="KB953_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HBB6867137.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         3860 a         1956 c         2501 g         3301 t
ORIGIN      
        1 tgaaatcaaa agaataaaat tccgatttat aatcgaaaaa agaagcattt ttatatatgc
       61 ttcttttttc gtagaatcaa taaatatgga tttggctaac tatggaagaa tactcgtatt
      121 agttttcagc gttcatacta gttgttacat tgtttgattg gccaaaagaa tttctgatat
      181 attttgtgct aaataaagaa aaagacgctt gaattgggaa ataagaaaac tactctatca
      241 ttacaaattt tcataaactg gcagatgttc taagtaaaca agtatctttt caaagtcgga
      301 aaaatccata atcaacgtag tggtattgat atttggatga aagcctacgg tatcttgacg
      361 atcaatagca gcatcgatca cgacttggat ttttttctct ttgtcgtgca tcaatgctaa
      421 tggagtcact gtacctgctg gaacgcctag gaggtcagga agctgttctt cagaaacaaa
      481 agataagcgc ttttctttta attgttcagc tagttgtttt aaatcggcgt gcttttcatc
      541 aggcaagatg acaagataga tttgttttcc ttttcttgat ttcaataata gattttttac
      601 ttgcggacca ggtagggaaa aagggacatt tttgactgac gtaatcgcag gatgatcgac
      661 tttttgaaaa gagataccca gtttttttag tagttcataa gcttcttgtt cggttgcacg
      721 actcatcgcg catcaccctc ctttttttat tagtgtatca taaaaaagag ttgacaaatg
      781 aaaaataatc atgtaaatta cacgtaaagt taaaacaaat gacatcgaaa agaagagtag
      841 ccgttaggtg tttttagaga gtctctggtt ggtggaaaga ggcagcagtt aaccgtgaaa
      901 cacatcttgg agtcgatttt ctgaagcgta tagtaggaaa atccggggag cccgttacag
      961 ctgcggtcgt tgctagatga ccagataagg ctggcttttc cgtgaggagt cggtaaaaaa
     1021 aggtggaacc acgtgattaa cgtcctttga tcttcggatc aaaggacttt ttgttttcat
     1081 ctgaacggca acgactgtgt gaggtttttt ctgcaaggaa aaaataaaca gaggtggaac
     1141 cacgtataat acgtcctctt ggcatttgcc aagaggattt ttttatttta aaggaggaaa
     1201 agaagatgga gtatttgatt gaagttatgc cgcaattatt agtaggagca ctcacaacat
     1261 tgaaattatt tttatttacc ttgattggct cattaccatt agggattgtt ttagcattag
     1321 gattgtcttt gcattttaag ccgctacgct acttgcttca aacatatgta tgggttatgc
     1381 ggggaacacc gctgttgttg caactgattt ttatttttta tgggttgccg acgattggga
     1441 tcgtttttga tcgtttcgaa gcagcactca ttgcctttat cttaaactat gcagcttatt
     1501 ttgcagagat ctttcgtgga gggatacaat cgattcctca aggtcaatat gaagcagcgc
     1561 aagttttgca attaagtccc tttcaaacgg tgacgaaaat cgtcatccca caagtgacaa
     1621 agattgtttt accttctgta gggaatgaag ttatcaactt ggtcaaagat tcttccttga
     1681 tctatatatt aggtttaggt gacgtaatgc gtgctgggaa aatcgcgatg gaacgcgacg
     1741 cgactttact gcctttgcta ggcgtggcaa tcatctatct tacattgact ggtattctaa
     1801 caatcgtaat gaaacaagtt gaaggaaaat taaattatta ccgctaactg ataggaggaa
     1861 gaaatatgat tgaattgaag aaagtgaaca aagcatttgg tgaaaaaaaa ataatcaaag
     1921 atttgaatct aatcattcca gatgggaaaa cattagctat cgtaggtcct tccggtggcg
     1981 gtaaaaccac gcttttacgt atcttggctg gcttagaaag ttctgacagc ggagaatttt
     2041 atttagacgg tgtaccgttc aacccattcg agcttaaaat gaaagaacaa gttgtaggag
     2101 tcgtattcca agacttccag ctatttccac atctgaatat ttttgaaaac attaccttag
     2161 caccaaaaat ggtattgaaa cagacaaaag aagaatacac caaaaaagca gaagaattag
     2221 cggcttcatt agggattagt gatttattga atatctatcc ttatcagctt tctggcggac
     2281 aaaaacagcg ggtagcgatt gccagagcat tggctatgaa tccacgggta ttggcttatg
     2341 atgaaccaac aagtgcttta gatcctgaat tacgccagca agtcgagcgt ttgatcctat
     2401 cattgaaaga agatggggta acccaaatta ttgtcaccca tgatttagta tttgcagaaa
     2461 atgtcggtga tcaattacta aaagtttccc caattcatta agaaaggaga agatagagat
     2521 gaaaaaaacc agttttctat tcgctttgat cgcaggtctt cttctttttg caggctgttc
     2581 gaacaaaaaa acttcagcag ataattgggc gactatcaac gaagagaaaa aaatcgttat
     2641 aggtttagac gatacatttg ttcctatggg atttagagat aaagacggga agctcacagg
     2701 atttgatatt gatttagcca aagcagtctt tgaacaatat gggattacgg tagactttca
     2761 gcccattgac tggtcaatga aggaatttga actgaacaat ggaacgatcg atttgatatg
     2821 gaacggctat tccaaaacgc cggcaagaga aaagaaagtg caatttacaa aaccttatat
     2881 ggaaaacgat caagtgctga tcacacctaa agaatcagga atcacaaact ttggacaaat
     2941 gaataataag agattaggtg cgcaaaatgg gtcatctggt tatgatgtat ttacgaaaca
     3001 accagaagta ttaaaagaga tagtcaaaaa ccaagatgct gttttgtatg atagttttaa
     3061 tgaagcattg atcgatctga aaagcagacg ggtcgatggt ctgctgatgg ataaagtata
     3121 cgcagactat tatttgaaac aaagaaacga actagaagac ttcaacataa caaaaggttc
     3181 ttatgagagt gaggattttg cagttggtgc acgcaagtca gatagtacat tagtgaaaaa
     3241 aatcaatgaa gccctagatc aactagagaa aacaggtgaa ttccaaaaaa tatccgataa
     3301 atggtttggc gaagatgtta cgccgagaaa ttaacaaata ggggactgtg acaaaagacg
     3361 tgtcacagtc cctttttccg aataaacagt gttctcctgc caactccttg tgactcggag
     3421 ctgaatggca ggctcacaac ctcttggtaa acggtgttca aaagccagtt tttcggtatt
     3481 aagccaaatt tatgcaaaaa tgtagaaagc cattttcaca aaatttttct tactactcga
     3541 aactaagcga ctttttcaca acctccttta cataatttta ttttgttgtg gaaggggttt
     3601 cgtgttattc taaaagagga acgattgaag gttataggaa aggaagagat gaatggaaca
     3661 caaagaactt acctcatttc caaaagattt tctgtggggc tctgcttctg cagcgtatca
     3721 agtagaagga gcttggcaag aagatgggaa aggagaatca gtctgggatc gatttgtacg
     3781 gatacctgga aagacattta aaggaacgaa cggtgatttg gcagtagacc actatcatcg
     3841 ttataaagaa gatattgctt taatgaaaga acaaggtctg aaagcttacc gcttctctgt
     3901 agcttggaca cggatttttc ctaatggacg aggagaagtc aatcaagctg ggctcgcttt
     3961 ttatgagaga ttgatcgacg aattgattga aaacgatatt gaacctatgt tgactttata
     4021 tcattgggat cttccacaag cattgcaaga tgaatataat ggttgggaat cacgtcagat
     4081 cattgaagat ttcactcatt atgcagaaac attatttgag gcattccgcg ggaaagtcca
     4141 ttactggatt agtttgaatg aacaaaatat cttcacttca ttaggttatt tgttagcagc
     4201 acatccgcca ggtgtgacag acccgaaacg aatgtatgaa gttaaccata ttgctaattt
     4261 agcaaatgca tctgtcatca ataaatttca cgagttagaa atgcctggaa aaatcggacc
     4321 aagctttgcg tatacgccaa attatccaat tgacagtaat cctgaaaatg ttctggctgc
     4381 tgaaaatgca gaagacctga tggccaatta ttggatggat gtctatatgt ggggaaaata
     4441 tccgattgct gccatgaggt tcttagaaga aaaaggatgg gcaccaacga ttgaagctgg
     4501 cgatgcagaa ttgctagaat ctgctaaacc agattttctt gggatcaatt actatcaaac
     4561 ggcgacgaat gcattcaatc cattaaacgg agttggtgct ggaaaaatga atacgacagg
     4621 aaaaaaaagt tcatcagaag aaactggagt tcctggaatg tataaaaaag ttgagaatcc
     4681 atttgtcgaa cgaacaaatt gggactggga gattgaccca gaaggattaa ggatcggact
     4741 gcgtcgtatc acaagccgtt accgcatgcc ggtactgatt acggaaaatg gcttaggaga
     4801 gtatgacaag ttgacagaag ataaacaaat ccatgacgac tatcgaatca attacttaca
     4861 aagccacgtc aaagccatca aagaagcaat ttcggatgga gcagaagtcc taggctattg
     4921 cacctggtct tatacagatt tgcttagctg gctgaacggt tatcaaaagc gatatggatt
     4981 tgtatatgtt gatcaagacg aaacacaaga aggatctttg gaacgatata agaaagacag
     5041 ttattactgg taccaaaaac tgattgaaga aaatggagag aacctttagt tcacatactt
     5101 aaaacaagaa ggtacggctg gatatggccg tgccttttct tgataaaatc atttagaaat
     5161 taagaaaaac tgtaaaataa gctgaaaaat gaaagaaagc gtcagttctt tcaaacaaaa
     5221 ctttagttcc cttacttttt tttgtataat gaaacttgat gaaatgaggg ataataaatg
     5281 gcacaaaaga aacggcctac taaaaaaagg aaaaagacga aaaaaacgaa aaagcaaaaa
     5341 cagcagcagg aacaaatgct gacgattggt gttgggtttg ccttgatttt atttgctgtc
     5401 ttcggctttt taaaattagg ttttttagga atattgattg caaacggctt tcggatcatt
     5461 gcaggaaata cttaccaaat cctctgtctt ttattagccg tattaggatt ttggattgtc
     5521 attaagaata cagagttttc aattggtaaa aaccgacgct ggtttggcgg catactcttt
     5581 tatttcggta tcttattatt gttacatgcc catttatttg gcaaactcca tactggggaa
     5641 ccaaatatca tggggacgac atgggatttg ttagccagtg atataaaaca aagtcaagta
     5701 gacaataacg ttggtggtgg gatgattgga gcaatccttt atcattttac ttacttcttg
     5761 attgcacagc ctggaagtta tttagcagca gtgctattac tggcaggagg agcattcctg
     5821 ttcagtaatc tggaaggcta tcagctgtta aatggtatcc aatcaattgg agaacgagta
     5881 caggaattat tggaaggtga tcctgccaag caagctcgaa agcaagcggc aaaggaagag
     5941 agaatgaaac agcgcgcaga agcgaaagaa gcaaggcgct tagctgcaaa agaagctgca
     6001 gaatatgaga agaaaaaagc ggtgagccag caaagggaaa atactgctga tgaatggcag
     6061 gaaccagaaa atacagagcc ggagcagcta agttttgttc caatcgacag ttttcaagaa
     6121 aatatccatc ctgcgaatct agagaaacca gtaccagata caccaaaaca gaccaataca
     6181 gcagaaggtt tttcagatga gttgccagag gatgacggta cctcattaga atttgagatt
     6241 gaagcagaac aggaaaatca agactatgaa ttaccgacag ttgatttgct tgattctata
     6301 ccaactgtgg atcaaagtga tgagtataaa aaaattgaaa aaaatatcgg cgtattagaa
     6361 caaactttcc aaagtttcgg tgtcgatgcc aaggtcgtca aagccagctt aggaccatcc
     6421 gtcacaaaat tcgaagtcca gccggctgta ggggtaaaag taagtaaaat tgtgaactta
     6481 acagatgata tcgcgcttgc tttagcggcg aaagatgtcc ggatggaagc accgattcca
     6541 ggtaaatcac tgattggtat tgaagtacca aatggaaaga tcagtatggt ttcctttaga
     6601 gaaatcatcg aagcacaacc aaaccaccca gataaattac tagaagtacc tttaggacgt
     6661 gatgtttctg gacgggtaca aacggctgat ctttcgaaga tgcctcactt actcgtggct
     6721 ggttcgactg gtagtgggaa gtcagtagca attaatggga ttatcacaag tatattaatg
     6781 cgtgctaagc cccatgaagt aaaactgatg atgatcgatc cgaaaatggt tgaattgaat
     6841 atgtacaacg gcatacctca tctgttgacg ccggtcgtga cgaaccctag aaaagcagcg
     6901 caagccttac aaaaagtagt acaagaaatg gaagaacgat atgaaaaatt cgcggcaacc
     6961 ggagtaagaa atatttctgg ttacaatgaa tttgttcaac agaaaaactt ggaaaacgga
     7021 acgaaacatc caaccttacc attcatcgta gtgatcgtag atgaattagc tgatttgatg
     7081 atggtagcca gcaatgaagt tgaagatgcg attattcgtt tagcgcaaat ggctcgggca
     7141 gcggggattc atatgatcct agctactcaa cgtccaagtg tggatgttat cacaggaatc
     7201 atcaaagcaa atgtaccttc cagaatggca tttgcggtat caagcggtac agattctcgg
     7261 acgatcattg attctaatgg ggcagaaaag ctcttagggc gaggagatat gctattcttg
     7321 ccaatgggtg agaataaacc gatccgtgtg caaggagcat tcatttctga ccacgaagta
     7381 gaacgggtcg ttcaatttgt tacagatcag caagaagctc attatgaaga gaaaatgatg
     7441 ccgacagatg aagttgaaac gacaggtgct ccagaacagc cacaagatga gctgtttgaa
     7501 gaagcaaaag cacttgtcgt tgaaatgcaa acggcaagta tttcccttct ccaaagaaga
     7561 tttcggatcg gttacaaccg agcagcacgt ttagtagatg aactggaagc tcatggagtt
     7621 gttggcccgt cagaaggaag caagcctcgg aaagtattca ttgagcagga agaaacatca
     7681 gaaacgttgc cggacgataa tgacatgtaa aacttgtgtg atgaagggtt gaaaaacgaa
     7741 taaccaataa aaagacaagg catgatgata ccaaaagaaa aaatggtttt tttccatctt
     7801 ttctaatacg gtatgatgtc ttgtcttttt agtttttgtg agattaaagt tctattcgat
     7861 ttatttgtga agtattgcat taaggaaatg aatcatgtat agtatgtagc gaaactttga
     7921 gaaaaagagg gaaaatactt tggaaaaaca gtccatttat ggcttgacga atgaagaatt
     7981 aatcaattgg tttatagaaa acggcgagaa aaaattccga gctgcccaag tttgggaatg
     8041 gttgtatcaa aaacgtgtaa gtaatttcac agaaatgacg aatctatcga aacaattgat
     8101 cgaaaaatta tcggagcatt ttatcatcaa tcctctaaaa cagatggtcg tccaagaagc
     8161 ttcggatggt acagtaaaat atttgtttga actccctgat aaaaacatga ttgagactgt
     8221 actgatgaga caagaatatg gcttgtcagt ttgtgtgacg acacaagtag gatgtaatat
     8281 cggctgtacg ttttgtgcaa gcgggctgct caaaaagaac cgtgatctga ctgctggaga
     8341 aattgttgca cagatcatga tggtccaaca ttattttgat gagcgcaatc ttggcgaacg
     8401 cgtttcacat gtcgttgtaa tggggatcgg tgagccattt gataattacg acaatgtaat
     8461 ggactttttg catatcatca atgatgccaa aggattggca attggtgctc ggcacattac
     8521 agtttccact agtggattag cacacaaaat caaggaattc gctaataacg gcttgcaagt
     8581 caatctggct atttccttgc acgcgccgaa taatgaggtg cgaacatcga tcatgcggat
     8641 caaccgaagt ttcccaattg agaaattgat ggaagctgtg gatgagtatt tagagaaaac
     8701 aaaccgtcga atcacgtttg aatatatcat gttgaatcaa gtaaatgata gaccagagca
     8761 tgcgcaacaa ttagcagatc tattaaaaga taagaaaaaa ttagcatatg tcaacttgat
     8821 tccatataat ccagtcagtg aacatgatca atattctcgc agtccaaaag ctgatgtatt
     8881 gagattttat gatgtattga agaaaaacgg cgtaaactgt gtgatacgta aagaacatgg
     8941 tacagatatt gatgcagctt gcggacaatt gcgaagcaag caaatgaaaa aaacagttgc
     9001 caaataatcg tatcctactc gttttttcaa ttaccataaa aggagcagtt ttcttaatta
     9061 aggaactgtt caacttaaat aagagaaaag attctatagc gaaagctata aagctttttt
     9121 tgacaagaaa aaccctcatc aagcaaagaa atgtcctcaa tttgatgaca gcctgcttga
     9181 tgggggtttt agcaattatt taactgattt tcttatatcc ttgctggctt tcttgtgtag
     9241 catgataagt ttctacgtca agaatggcct gtttaaattt ttctcgggtg atttcataag
     9301 gcacatgttt aatatcattt gttgccatgg ctttatctaa gattgcttct aaatcggtcg
     9361 tgacattgag attcatgtcg gttaacgata ctggaagttg gttctttttc ataaacacat
     9421 agattttatc aaacaaatcg aactgctgat ccatcatcaa taaggtcagt acaccataac
     9481 tgacagatgc tccgtgaagg tatccctcca ttttttctag aacagtcgta ccgtaataga
     9541 atgagtgtgc catagcagaa ttatagttat tgttaactaa aacagaaacc aatccggtag
     9601 taacaatgat atccgaaacg acttgttcaa ttgcatatga gggacgatcg tcggcactat
     9661 ctttcaatgc ttgcgtacca taagtcaaga gacgatcaat gcacattttt ccgatttgga
     9721 ttcctaatct atccgtgtag cctagatcat cgccacgagc agaaaaagta gattcgactt
     9781 ctttggataa agcatcgcca atacctgccc ataagtattt atcaggtgct tcggcaataa
     9841 ttgctgtatc aatgaaggtg tgtacaggtg gttttgaagg aaagaagagt ccgtccatta
     9901 cgccttcttc gttatagatg acacataccg cagtgacggg cgaacagttc gaaccaatag
     9961 tgggaaaggt gaagactggt ttagctagtt tttcaccaat gacttttaca gtatcgcaag
    10021 cacggccacc accgactgca aacaggacgt ctgctttttg gacactttca tgctggatca
    10081 actgttcaat atttccataa gttgaattac ccccgtacca gagactggcg acaacctcta
    10141 tttcctgtgt caaagctact tttattttgt tttctgcctt tgaaagtgca gtcttccctc
    10201 caatcagcac tacttttgtc ccatattctt tgacgattcc atttgctttt tcatacgcat
    10261 ctgttccaat ggtataattt ggtaagaaaa cactttgact catattcatt tccttctttc
    10321 ttcgttgaaa cgggttaaaa ttttaaagag tgatcgatct gactgcggct gtagatggag
    10381 tagaggtgaa cctaagacag gataagtgat gaataaggtg gcatgaacac cttgaaatca
    10441 tcaataagta tcctaaaaac gaacaagtac aaaaacattg aaaacgacaa taatgaattt
    10501 ttgttcctct tagtatttct accgattccc tttgcttttt tgtaattatc taaaaattct
    10561 aaaaattaag attattatat gaaaacaggt tttatttgtc aattcataat tggttttttt
    10621 taaagcaaaa gataatctat atcatgatga acaaacagag ttttttcctc caattgatgg
    10681 ctttcctgat ctttcctatt ttgaataaag tgtttctgta taatttttta ttttataagt
    10741 aagataaata atgaaagaat agagatactg attggtagct aaagattttt gagcgagaac
    10801 atttcgtttt ttcaaaagga ctgttagaat aagcaagaac aagggggaag acgtatgata
    10861 atcagtgaat ttgaccgtaa taatccagta ttgaaagatc agctttctga tttactgaga
    10921 ctgacttggc cggaagaata tggagacagc tcggcagaag aagtagaaga aatgatgaat
    10981 ccagaacgaa tcgcggtagc agcggtagac caagatgagt tagtaggatt tattggtgca
    11041 atccctcaat acggtatcac aggttgggaa ttgcatccat tagttgtaga aagctcccga
    11101 cgaaagaacc aaataggtac tcgattagtc aattacttag aaaaagaagt agcttccaga
    11161 ggaggaatca cgatttattt aggtacggat gatttagacc atggaacaac gttaagtcaa
    11221 acggacctgt atgagcatac atttgataaa gtggcttcca tccagaacct tcgtgaacat
    11281 ccgtatgaat tctatgaaaa attaggttat aaaatcgtag gtgtcttacc aaatgcaaat
    11341 ggctgggaca aaccggatat ttggatggca aaaacgatta ttcctcgacc aaattctcaa
    11401 taataaaaag aggttgtgac agaagtaatc agcttcaaga aataagaagg aatttacgaa
    11461 aattacttct tgaatttttg tgaaattcca acttatttcc gaagaagcta cttttggaac
    11521 accgtgtacc agaggttgtg aaaaagtcgc ttagtttcga gtagtaagaa aaattttgtg
    11581 aaaatggctt tctacatttt tgcataaatt tggcttaa
//
LOCUS       DADVXU010000085        11611 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.089, whole genome shotgun sequence.
ACCESSION   DADVXU010000085 DADVXU010000000
VERSION     DADVXU010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.089"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(105..629)
                     /locus_tag="KB953_002174"
     CDS             complement(105..629)
                     /locus_tag="KB953_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB6867138.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="KB953_002175"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="KB953_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBB6867139.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="KB953_002176"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="KB953_002176"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HBB6867140.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="KB953_002177"
     CDS             complement(2351..3031)
                     /locus_tag="KB953_002177"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBB6867141.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="KB953_002178"
     CDS             3157..4026
                     /locus_tag="KB953_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBB6867142.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="KB953_002179"
     CDS             4052..4567
                     /locus_tag="KB953_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867143.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5207)
                     /locus_tag="KB953_002180"
     CDS             complement(4614..5207)
                     /locus_tag="KB953_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB6867144.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="KB953_002181"
     CDS             complement(5232..5411)
                     /locus_tag="KB953_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867145.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="KB953_002182"
     CDS             complement(5488..5997)
                     /locus_tag="KB953_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867146.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="KB953_002183"
     CDS             complement(6093..6473)
                     /locus_tag="KB953_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBB6867147.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /locus_tag="KB953_002184"
     CDS             6803..7552
                     /locus_tag="KB953_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HBB6867148.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7736..9013)
                     /locus_tag="KB953_002185"
     CDS             complement(7736..9013)
                     /locus_tag="KB953_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867149.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="KB953_002186"
     CDS             complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="KB953_002186"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBB6867150.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9935..10879)
                     /locus_tag="KB953_002187"
     CDS             complement(9935..10879)
                     /locus_tag="KB953_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBB6867151.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10881..11567)
                     /locus_tag="KB953_002188"
     CDS             complement(10881..11567)
                     /locus_tag="KB953_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBB6867152.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
BASE COUNT         3307 a         2462 c         2044 g         3798 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaataacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct attttttata
     7741 gtaattcctt cagatacttt cttgcctcat cattgaaacg cagtgattct tctaacttct
     7801 gataatattc caacgcttgt ttctgagctt cagtaaaacg ataatgtttt cctttccatt
     7861 tgtcgattgt ctcttgcaca tttccagatt ggatatcttt cactactagt tgccattttt
     7921 cttttgagac tgtacggtct ttcttatcag cagattgtat agcttctcgt tctttacgca
     7981 atacttttaa aaaaccattt ctgactatcc gaggaatgct gtcgaagaac ccttctgtaa
     8041 aaccatagcg acaatattgc ttccaaataa atagatagat ttttggttgg atgaatcgtt
     8101 ggtttttcgc aaaatattcc atctgagcag tgacttctgg tgttcgttga cgttttgtct
     8161 ttctcaacac ggaaataaat gtttcaaaat aagcattagg atgtactggc acttcttcat
     8221 atacgatgga ttgccaatct ttacgtaaaa atgcgcgata gttgatcaag aattcttcta
     8281 catcctttac taattcttct gcaaagtcgt caaatagttc tttttttatc tgtcgctttt
     8341 ttgactgccc attgatgtgt tcacttattt tcttgatttc attttccaat gcttgataat
     8401 ccgctatgta aatcggcatt tctacttggc gaaattctgc gagccgctga gctaatttta
     8461 tatccggtaa atatggctgt ttcatccgac gattttggta caagacaaaa cagtattttt
     8521 gccatagtcc ttcaggaaaa tcgatattct tctgcttaag ccataataat tcgtctaagg
     8581 ccagatcaac ttttgtcatt ccctgatgga tcgaagctgc cactgtcgga gagacatgca
     8641 ggtgatcggc aaagtggttg atgcctagtt ttaggatctt tccggttttt ggcgatttaa
     8701 cgatgtattg cacttttaat tctctaccac actcacaata caatttttgc tggcttcgtt
     8761 ttgttcgaaa aaacggattg atcttgaagt cagaaaacac ccaatctgtt gcagctaaat
     8821 aatgttcttc cataaaacga gagctgatta gatatcggcg gtgcttatcg actagttccc
     8881 gctgtttttt cgttaatttt gcatagatct cccaacgttg actgcttgtg tattgtttag
     8941 aaattttgat gggatagctt ttgctgattt cttttggaaa ccattcttca aagacggatt
     9001 gccattcttc cacggctgtt tctttccttt ctaattacaa aaagctggct actaacataa
     9061 cgttagcaac cagccagtgt ttcttgctaa attagtgagt tgttccggca tttccggaaa
     9121 tatcttcttt attttctact gctgcttcaa ctgcataagt caaagaagaa gcaatgtctt
     9181 ctaaagacat agaaggcgta cccactggac gatcgatcac ttgatcgggt gagaatggca
     9241 cgtggatgaa cccacctcga attgccggga attgtgtttc gatcaaatga agcaaacgat
     9301 acatgatatc attgcagaca aatgttcctg ctgtataaga gatgtacgct ggtagaccat
     9361 gatcttgtac attttttgtc attgctttga ttggcaatga ggtaaagtaa gcggctggac
     9421 cgttttcttc taaagccgta tcgaatggct gatttccttc attatcaggg attcttgctt
     9481 ctgctagatt gattgcgaca cgttcgaacg agactgctga ccgcccacct gcttgtccga
     9541 cacagatcac gacatctggt tggtgctgtt ggatggcttc tttgactact tgactgcttt
     9601 tttcgaatac ggtcggtact tccactttga tgatttcagc accgctgatt tcatctggca
     9661 ttttttttac tgcttcatag gctggattca ctttgtcccc accaaatgga tcaaaacctg
     9721 ttactagtac tttcatctgt tgttcctccg tccgattgat ttaacatatg gttatctttt
     9781 atcataccat tttccttgaa cagcgtaaat aaaggaaaca atagtttaaa taacataaca
     9841 gatcaaactt aatatattct tataaaaaaa tagtgttcca ctacaatgtt agaagaggtt
     9901 gcgacaatta ttctgtcaca acccctctct tctattattt aaatagaatc atataagcaa
     9961 tttggaaaac tagcataaat aacgcaataa acaactggtt tttgatcact ccgtatttgt
    10021 ctttcatctc cagcatcgct acaggcacga tattgaagtt agcagccatc ggtgtcagca
    10081 gggtcccaca aaaaccgcaa gtcaatgcaa ccattcctat taacgctgga ttcgctccat
    10141 gactaaatac gaatggtgca ccgatcccaa ccgtcatcac ggtgatggcc gcaaatgcat
    10201 tccccatgat taccgtaaat atcaccatgc caagagcgta aatgatgatg ccgatgttca
    10261 catttccttc tggaacgatc gtacctactg cattggaaat cactgtccca actccagcgc
    10321 ttgtaaatac cgcacctaga gaagctaaaa gcattggcaa catgcttaac ggccctactg
    10381 tgcccagcat atctgctgca tcatctaaaa atgtacttgg tcgattatct cgagaaaaga
    10441 acatcaagat tagcatggct gcaaaaacac cgacgcccac gccgactaaa gcacctaaat
    10501 tggtaaatag agcaaacaag atcgcaaaga caccgatact caaagctgga ataaaaattt
    10561 tcattcctag tttgtctgac attttttcca tatattcttt tgatggaagc cggctttctc
    10621 ctttgctgac tttcttagca attgctggta tggtcatcgc aaagatcaag ccaccgttca
    10681 cgattgccgg aagccatctt cctcctgcaa taactacccc taagaccgtc cagaaaaagg
    10741 ctgacccata acgatgagga ttggttttat ctgttaaatt tcttactcca gtgtaaatca
    10801 atacgagtcc catcacgata taaaggatct ccagtaattt atcgcctaat aaaatatcag
    10861 gatttgtaaa aaagtccatc ttattgcccc tttcctggat aatatttctt cgctaatttc
    10921 ttttctttaa gatagaagta gacacttgcc acaactaaag ccgtgatagc cactggaact
    10981 tccactaaag caagctgtcc caatgttacg tcatatccta gatctttcag tgttgattgg
    11041 actagcaacg cccctgctcc tccgatgaat agtacttggc caaagaacca gcagacattt
    11101 tccatagctg aagccatacc ttttagttct tcttcatgct taggatggac ttctaaccct
    11161 tttgattcga tggtccccaa tgccatcggt aaaatgattg gacgaacaaa tccagcaact
    11221 ccaccaaaac tcacgttaaa agccgcaaag atcattcgaa agatcccata aatatctaca
    11281 atcataccag cagatacttt cttgaatcgc ttgatcaagg aagccgctga ttctttcaga
    11341 ccatttcttt caagcgttcc tgttgccaac ataatgatga taaaaatcgc catcccgcga
    11401 ttgtttacaa aacttgtacc taaaagttct aacattcctt gaatgccaag accgccagtc
    11461 aaagctgtaa caactaaagc aaccattacg atcaatatcg agtctaattt aaatgcaaac
    11521 ccgagaataa caatcaagac gcctaaaagg ctaagataat tttccatata ttttcctcca
    11581 taaaatgaca atattttttt aattataaag c
//
LOCUS       DADVXU010000086        11381 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.090, whole genome shotgun sequence.
ACCESSION   DADVXU010000086 DADVXU010000000
VERSION     DADVXU010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.090"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(25..477)
                     /locus_tag="KB953_002189"
     CDS             complement(25..477)
                     /locus_tag="KB953_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HBB6867153.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            627..1577
                     /locus_tag="KB953_002190"
     CDS             627..1577
                     /locus_tag="KB953_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBB6867154.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            1570..2529
                     /locus_tag="KB953_002191"
     CDS             1570..2529
                     /locus_tag="KB953_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBB6867155.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            2526..3281
                     /locus_tag="KB953_002192"
     CDS             2526..3281
                     /locus_tag="KB953_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBB6867156.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            3304..4257
                     /locus_tag="KB953_002193"
     CDS             3304..4257
                     /locus_tag="KB953_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HBB6867157.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(4901..5197)
                     /locus_tag="KB953_002194"
     CDS             complement(4901..5197)
                     /locus_tag="KB953_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HBB6867158.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            5552..5908
                     /locus_tag="KB953_002195"
     CDS             5552..5908
                     /locus_tag="KB953_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBB6867159.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            5918..6817
                     /locus_tag="KB953_002196"
     CDS             5918..6817
                     /locus_tag="KB953_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HBB6867160.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            7334..8065
                     /locus_tag="KB953_002197"
     CDS             7334..8065
                     /locus_tag="KB953_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBB6867161.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            8210..10237
                     /locus_tag="KB953_002198"
     CDS             8210..10237
                     /locus_tag="KB953_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HBB6867162.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            10345..>11381
                     /locus_tag="KB953_002199"
     CDS             10345..>11381
                     /locus_tag="KB953_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HBB6867163.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFL"
BASE COUNT         3670 a         1915 c         2314 g         3482 t
ORIGIN      
        1 taaaaattta tgtgtttttt cgttttaaaa taaacgatcc cagaagcttt ttttcttttt
       61 tggtttttct ttttcagctg gcgaagagtt ttcttctggg tatttttttt cgatatcaat
      121 cacaggttca tttactgctt cttttgctgt taagagcaat ccgaattcct caggatttgc
      181 agtgacgtaa tcgttgacta cagtgaaacg gctattgttt ttcgttgcta gctggatata
      241 ttggttttgg acagaaaggg gtagagcccc attcaagagt atggaagcat ctggatgttt
      301 tttgagttct tttaaaaagt tttctttgtt ttttggttgg atcatttcag caacagtcat
      361 actaagatag cagcgttccc tgaatgtccc taaatattta tgctgttctt cagggttcac
      421 taaaggtgta ccataaagtc ccttatccaa atggcttttt acatcatctt gtgccataaa
      481 aaataccctc cttcttctct ttgtctttct tacgattata gcatattccc aataatacct
      541 aaaacatatg aaaaagcggt tgcttttgtt caacgattca cttaaaataa gaatgaaaat
      601 cattctcaat tagaaaggtg gattggatgc gctacggacg actagtagca ttattagtta
      661 ttttaagtag tatttcattg ttcatcggag tatcgaatgt acaattgtca gaactgctcc
      721 agttagatgg gcaaagctta caaatcttgc tagtgactcg tttgccacga acgatttgtt
      781 tgatccttgt gggagcgacc agtagtattt gtgggttgat catgcaacat ttgacccaaa
      841 ataaatttgt ttcaccaacg actgcgggca cgatggatag tgcgcgttta ggtattttag
      901 tggctatgat tttcctacct ggtgcatcct tgattgtccg atccttgaca gcattttgct
      961 ttgcttttgc aggaacattg ctttttttat cccttacgcg attgttcctc cagaaaaatc
     1021 aattgatgct tccgttgatt ggagtcatgt tagggaatat cattggttca gttgctactt
     1081 tttttgctta tcaattccag ttggtacaaa atatgacttc atggcttcaa gggaattttg
     1141 cgactgtgat acgaggaaat tatgagttat tgtatgctgt tgttccattg ttgctcttgg
     1201 tttggctatt tgcttaccga tttacgatca ttggtctggg cgaatcgttt gcagtaaatc
     1261 tgggaataga ttaccagaag atgcagtttt taggcattgg cttagtatca ttggctagtg
     1321 cagtcatgtt gatcatggta ggaagcattc cttttatcgg agtgatcatt ccgaatcttg
     1381 tctctagaat atatggagat caagtgcaca aaacaattgg cgtaactgct ttagcaggta
     1441 gtttgttctt agtattttgc gatcttgtcg ctcgcgtgtt gatttttcct tatgaaatac
     1501 cagtcagtgt gatcgttgga atcatcggtg gggctatctt cctttatctt ctgataaggg
     1561 gacgacgata tgcttaaaaa aaagccatct atttttattg gtatgcttgt tcttgtttta
     1621 tttgttacag gaatgtactt gacgatccat acatatggaa actggcattt tgcatgtgca
     1681 ttgaggagta agaaaatttt tgcatttatc cttgtctcgc tagctgtcag cagtgccaca
     1741 atcagctttc agacaattac gcataaccag tttttgactc cttcagtatt gggacttgag
     1801 tcgttatatg tccttgtcca aacggtcctt ttcttcttta tcggaggagt gacgatgctt
     1861 cagcagacag gtacagtttt tttcttattg aacgttgggc tgatggtcgg gctgagtcta
     1921 ttgttatttt tctttctttt gaaaaaaatg agagcgaatc tttttttatt actgatgtca
     1981 gggatgattt taggtacgct ttttggtagt gtcagtacct ttttacaagt catcatggat
     2041 ccgaatgaat atgatttgct tcaaggcaga ttatttgcaa gctttggcaa tatccagagt
     2101 cagtatcttt tgcctgcttc tttgatcatt atcggctgct ggtacctgtt atttcgatat
     2161 cgacatgtgc tagacgtttt ccatctcgga aatgaacaag cagtaaacct cgggatagac
     2221 acagatcgtt ttgaattttt gattttatgt tgtgtcagtc ttttgacagg gacatcaacg
     2281 gctcttgtgg gtccagttac ttttttaggg tttattgtcg caaatatcag ttatcgtgtc
     2341 ttttctactt atcgtcatgg tcagctattt atcggaagct ttctggtagc tatgctgttt
     2401 ctgacaggag gacagctcgc agtagagcag attttcggct ggaatacaac agtgagtgtc
     2461 gtcgttgaat ttgtcggagg aatttatttt gtcacaaaat tgattcgcga aaggaagagt
     2521 atcctatgat tgatttacaa aaggtcaata aactgtacca tggaaaatca gtggttaaag
     2581 atgtacagtt aacgattcct gaaaagaagt tgactgcttt tatcggtcca aatggtgctg
     2641 gaaagtcaac ggtgttgtcg atgatcagtc gtttgatacc aaaagataca ggtgaaatct
     2701 atttagacca taacgaagta aaggcttgga aatccaatga attagccaaa aaattatcga
     2761 tcctacgaca atcgaatgaa ataaatgtcc aaatcaccgt gaaagaatta gtagaatttg
     2821 gacgatttcc ttatacgaaa ggaaaattga cgacaaaaga cgaggaaatc gttcaggaat
     2881 cactggaaca ccttgggtta gaagaaatag cggatcaatc gattgacact ctttcaggcg
     2941 gtcaactcca acgtgcgtat attgcaatgg tgctagcgca agacacggat tatattttac
     3001 tggatgaacc tttgaacaat ctagatatga attttgcggt tcaaatcatg cagattttaa
     3061 aaaatttagt acaagaaatc gggaaaacaa ttgttattgt tcttcatgat atcaactttg
     3121 ctgcaagtta tgcagacgaa attgttgcaa tgaaagatgg acagttattt acgcaaggaa
     3181 cgacccaaga aatgataaaa aaagatgtat tggatgaatt atacgaaatg gacatacgga
     3241 tttgtgagat tgaaggccgc agattctgtc tttatttcta aaactaacat taggagagaa
     3301 gagatgaaaa aattagtagt aggtatcttg attctagcat catttggttt agcagcatgt
     3361 ggaaatagca acaacaccag tcaggcagac acaaaaaaaa gcagcacaca aactgaaact
     3421 acattgacga taacagatag taatggagac caaatcgagg tgccaaataa tccagaaaaa
     3481 gtcgtcgtat tcgacaatgg ttctttagat acgatggatg ctcttggtgt tggagataag
     3541 gtagttggag cagctacaag cagccttcct gagtatcttt catcctataa aaaagtagaa
     3601 tcagcaggtg gaataaaaga accagactta gaaaaaatta atcagcttca gccagatcta
     3661 atcatcattt ctggccgaca aagagatttt caaaaagatc taagtcaaat tgctccgacc
     3721 attttccttt ctttagatgc gaaaaatcca tgggaatctt tccagcaaaa tgtcactgct
     3781 ttaggggaga tctttggtaa gcaagaagaa gctaaaactc aattagagga attatctagt
     3841 gcaatcgatc agacgaaaaa gaaagcagaa gctactgaca aaaaagcact agtcacactt
     3901 gtgaatgaag ggcaattatc agcctacgga tctggttcta gattcggttt catccatgat
     3961 ttatttggct ttgaacaagc agatgatcaa atcgaagcat ccactcacgg tcaaagtgtt
     4021 tcttatgaat atgtgctgga aaaaaatccg gatatcttat ttgtagtaga cagaacaaaa
     4081 gctattggtg gagacgacag caaagatgat atttctgcca atgaactggt agcacaaacg
     4141 aatgctggta aaaatcagca gatcatctcc cttgaaccag atgtctggta tttaagcggg
     4201 ggcggtttag aatcaatgaa actgatgatc gaagatgtga atcaagcatt taaatagcat
     4261 aaataacaga ggattaaact ctcatactat gtaacaataa tgtttcttac attcttaaat
     4321 agcaagagcc tatcctcaaa gaagttatca aaaaccatcc gtaaaatcgt attgatgatt
     4381 ttacggatgg tttttattta tttgagaaat cttttttcaa cgtgaattca ttatctacat
     4441 catacgggtt gattctagaa tggggagtgg caaaaaatag tgtgatccat tttttcagca
     4501 tcaagatcgc ctcctttgat ttgaatcaaa ggtacaaagc gaaacttaga taaaacttaa
     4561 atttagatat ttttattttg taccgatctc ttctttaaac gatgttccat ctgctgcttc
     4621 tcggcaataa gtccactaga ctagaaaata tgagaagcta ttttctgtcc agcgttctta
     4681 ttactcaaaa caaatcgcag ttgtcacaat ctcttctaaa cgatgttcca tctgctgctt
     4741 ctcggcaata agcccactag actagaaaat atgagaagct attttctgtc cagcgttctt
     4801 attactcaaa gcaaatcgca gttgtcacaa tctcttctaa acaatgttca atcagctgac
     4861 cttcggtatt agctcaaagc actgatttca caacctctat ttaggcatgt gtctcagcaa
     4921 gcggttgata gttgatgatc tgcatttttc gataaaaatc ggcgggaagc ttactccacc
     4981 gttttagtaa ttgatagaaa taactctctt ctgctttgat tttatactcg cttaatatca
     5041 atccaaaact attcgttttt tttgaagcta attttttata aatatccaat gctttggttt
     5101 tatcttcatt gacaaagagt gtttttactt caatataaga accctcgata ttttgtgttt
     5161 cttcgataaa atagatgttt tttatatatt ccttcatagt gattcctcca aagaagtctt
     5221 ttatttgata agtttactct accgaagagt tgttaataat ccgtgaacaa aaagtaattc
     5281 tttttccaat agttgttaaa ggttttttat tttttatagt attttttgtt atttaagaaa
     5341 ggaataaggt taaacaaata acaggttgct ttttgggtct ttttccgttc aagaaactga
     5401 acgaagaaaa gggaaagttg tctcttttgt gataagagaa atcataataa acgaatgact
     5461 tactttccaa taaattgaga acagtcagaa ccagacaaat tagttgcatc tgatgaataa
     5521 agtgattatt ctatgacatg aggtgataca gatgaaatta gaagtgacaa aagaagcaca
     5581 agaagtattg aagaataaat tagaagcaga cgatcaactt ttattagata tcgaaaacgg
     5641 tgacggtcca tttgcagaaa gccaagtcag ttgccaattg gatacggcat ttcgattgat
     5701 cattgtcaaa aaagatacac aaacagatct aagtctttat tctgtagtgg ctgatacacc
     5761 agttggacca atcaaaatca aagacagtgc aatactttac atggatgatc catccacact
     5821 tgctttagaa ccaacttata atagtttaca attaaaagga ccaagcggct tgagaaaagg
     5881 caacttgcaa gttgttcgaa aagactagag ggataaaatg ggaaaattgg cgatcatcag
     5941 cgacctacat gtggacatca ataaattcgg cgaatcagag ctgatgcagc tttggcaagt
     6001 tttgcaaaag cacaagatca ctcggcttca tctagccgga gatacggcta ataaagtaga
     6061 ccgttgccta gctgttgtcg aatttttcgt caataaagga ttatctacga catttcattt
     6121 cggcaatcac gagttggcag atgtgcccaa tgaacagatg atggaccatt ttccagaccc
     6181 acatttttta aatgaacggt atattgcttt aaatgagcag accgttcttt tagggatgaa
     6241 tggctggtac gactatcagt tttccgaatt gaaagattac gaggcaatcg tacgaatgaa
     6301 gcgtttgtat tggtatgatc ggttcatcca gcgtgggaaa acagatctcg aggtcaatgc
     6361 agaaatgctt gcagctgcaa aaaatttgct agaccagctg gaagcaaaaa aacttgaggt
     6421 gattttatcc actcatttcg ttcctaaacg ggcatttatc atgtatcaat ctgctcctta
     6481 tgagcgttgg aacaaattaa atgcattttt aggatcagaa tcattcggag aattacttga
     6541 ccgttattca aatatcaaac aagtagtttt tggccatacg catcgtcgct ttgaagataa
     6601 atccatcggt cacacgattt acagctgcag accttttggt tattattatg aatggcagct
     6661 gacccgagat tttgtcttga aagaacagtt gctagacagc tatgatccta tgaaattacg
     6721 tgtcctcttg cgtgcccatc acccagcttt tcaggcctat caaacacaac atttgcaaga
     6781 ggaatttgaa caagcgatga caatcgttcc gtattgagac agcattagta atgtgtaaaa
     6841 gaaaatacct tgtaaaacat aaggataagt aagagcagta gatccattta tcgtggatat
     6901 gctgcttttt ttgcggaaaa taatcagcga atgtttattt gatcggatga tttgtaactc
     6961 gcagagtact agatgcaagc gtgtattctt tttttgaacg ataatcacaa aaaaaaatat
     7021 aatatacatt ttttatttaa ttataattaa tattgattga agtctaggca gtcaagtcac
     7081 attttgatag ataggagggg gacttacgaa ttattttaaa ggctatgaca gctgtatttc
     7141 tagattgtgt ttgcatcaag ttgcaataaa aactagaaat caaatcgggc tgataatcat
     7201 ttataactaa tcgatacata ggaccaaata taggtctatg ttcttttatt tcataagtat
     7261 cgtaaaacag aattttgcta tggattaatc caatcgactg atatgtttag taaataaaga
     7321 aagaggaatg tcaatgatga aaaaagaaat cgtattaaca gcagcaatga tgttattcag
     7381 catggtagca agtacgactt ttgtttctgc aactgaagta tacccaaagg aatataacac
     7441 agaaggaacg attacatttg aagcaggaga tgagggtgta actcctcctg ttgatccaga
     7501 gaatcctgat cccaataaac ctgtcgatcc atcagatcct ccaagccctg gaacaggagg
     7561 ggcattatcg attgattatg gatcaaaatt caaatttggg actcaaaaaa tttctactgc
     7621 ggataaaacg tattatgcag ctgctgatgt gatgaacgat gggtctagaa aaccgacata
     7681 tgtccaagtg acagatagga gaagtacctt atctgggtgg aaattaagtg tctctcaacc
     7741 agaacagttt aaaactgcca gtggggatga actagttgga gcacagctga aatttacgaa
     7801 agggcaggct gtttctttgg ttgatccaac atatacgccg caaacggtga attctgaatt
     7861 aacgttgaca ccgggaggaa acaatacgtt agcaattaat gctaaatcag gaacaggtgt
     7921 ggggacttgg gtctatcgat ttggtgcaaa tgaaaatgaa aatcaagatg cggtacagct
     7981 ttcggtaccc ggtaaatctg tcaagttagc gcaacaatat tccactaaat tagtttggac
     8041 attagaagat acacctaaca actagtttac tgaaaagaac tgttaaaaat aaatacgtag
     8101 tcatattgtc ttgcgaggta atcgagcaaa aattgtcaaa ggctaggcta aatttgtttg
     8161 gatacgaata gttcatctag gaatttaacg atcaggagga aaagatatat tgaaaaagag
     8221 gaggatcgtg ctcattttaa gcagtctctt agtgactaat atctttccga tcatagggta
     8281 ctctttggaa ttgaatcagt tggagcatgg aaatagaaga gacgaagaaa gcaaactcac
     8341 ttcgacattc gacagtttaa tagaatccaa ccagacacaa gaaagcagtg aggatttaag
     8401 tatttcgagt agtggagaga ccactgacaa cgtagaagct gagggttcgg acaaacaagg
     8461 actagaagat gcaccaattt ctgcggttga gctgcctgac gatcccaata ttatttctat
     8521 cgataaaatt tttcaaacac ctatcggtac tagcacgtcc atattggaaa atgggaagtt
     8581 actgcagttg aacccagcag aaaggtctca aagaggagct atttggtcaa ataagaagat
     8641 tagtttactc tcggatttta catttaaatc ttatttatac ttaggaaatc aacgagatag
     8701 agcgggagat ggcatgacct ttacattgac tggcgatcct agaatggcta gtaatcccag
     8761 tcaagttatt ggatcgccag gtatgggaat tggctcatat tctactcgtt ctggccaacc
     8821 ttacattgcg aatgcgctct cgattgagtt tgatacgtat aaaaatagtg gttcttctga
     8881 tcgaatggat cgagagataa ctgctgataa tagagggcat ggacatgtcg cttttgtcac
     8941 gcctaaacca aataataata attattctgg agagcatact ggcgtgacgg tagctcctac
     9001 atttctttct aatggcacat ggcgcttgtt aataattaaa tgggacgcgg agcaaagaag
     9061 tcttacttat gatttggaag gtgtcggtcg ctcttctcgg attatacctg atctcattag
     9121 gcagtttggt tctacagaag taaattgggg gtttacttct tcaacaggta ctttttttca
     9181 agaaaatgct gtcgctgtca cgcgacttcc atcgaatgtc acatcaacag ctgatgtaaa
     9241 agtcaacgat agtgattatg gggccatcgc tgaagctgga ggaaacgata agataactat
     9301 cagaaataca ttgaaggtcg agggggattt tcttgaatat aagaaaatgc agactagtat
     9361 cgacctccca caagaattaa ctattcccga gcatatcttg atcaatggaa atcaaattga
     9421 tgccaaagat atcttgataa ccggaaatcg agtcacgcta aatctgacag attattttat
     9481 tgctggagat acgttaataa tcgaattgga gacaactttg caggaagtaa ttcctgaaaa
     9541 agtactcact atgcattttg aataccttga aaacgggaat ttgattgagc aatcgaatga
     9601 aataaaccta aagatcgcaa agctgaagga gatagatatc catgtttatt ataaagataa
     9661 agacagtcag attgatattg ctgaaatgaa aacaatcact gggaaaatag gagagaagta
     9721 taaagaaact cctattgata ttgaagggta tgtttttgac tcggattcag cgaatacaga
     9781 aggcatcttc tccgaaaata cagaggatat ctatttttat tacagaaaag gcaaacttta
     9841 tctctcaact gctcctcaat atgtcaattt tggaaaacat aaaatatcca gtagaccatt
     9901 aagtgaattc ggacagtttt caggtgggtt gaaaattata gatgaacggg cttttggaag
     9961 ttggaaatta caattgaaac aagagaaagt tcttacaaat ggagattttg agcttccaga
    10021 agctctttcc tttgttacct ctgaaaatac aacggttata gggacatccg ctgttacgat
    10081 tttcgaaagt gatcaaaagg gtgaaagcga tttgtcctct cttttagatt ctacagctca
    10141 tcgtggaatc agaataaata ttcctgtaga aaaccaaaga atcggaacct ttgaggggaa
    10201 actatcttgg atacttgcag atgtcccagg aaattaacag gaagagaaga atataacaaa
    10261 attattgagt ttcaagaagg gcacaggtac cataggctct tgtgtcccac agagacaaga
    10321 ttttgttata gtcttctctt acatatgatt aaaggagcga ttttcttgag accaaaacaa
    10381 caattattta taacactcct agttatttta ggggcacttt ttaacaatat atcagtgtca
    10441 gctagtgcat ttaatttttc agtgacgccg ataacttcag agaatcaaat cgacaagaga
    10501 aaaacttact tcgatcttca acttgatccg gatcaggaag tagaagtaaa agcggaattg
    10561 agaaatgata cagaaaaaga agtgaaaatc gatatttctg tgaatagcgc aaccaccaat
    10621 tcaaatgtga tggttgaata cggaaaaaac gagattgaaa aagacatgag cctgatcttt
    10681 gatctggtag attatgtgag ctatccacag atagtgacgc taaaacctaa aagtgtccaa
    10741 accgttgctt ttcatgtcag gatgccgaat gagcgatttg atggtgtatt ggctggtggg
    10801 atcacattcc aagaaatcca gacggaaaag gaccaaacag aaactaagga tcaaagctta
    10861 tcgattgaga atgaatacac ctatattatt gctttgttga tgcaacagac actgaacgaa
    10921 gttgctccga accttactct tcatgaagtt aaaccagatc aaatcaatgc aagaaatgtc
    10981 atcttagcaa acgttcaaaa tgatcaaaaa acctatatca atcaagtagt tattgaaaca
    11041 aaaattacaa aaaaaggaca ttctgaagtt ttatatcaag aggaaaaaga aggattgcag
    11101 attgcgccga atacaaattt ttcttttcca actgctttaa atggtcaacc gctaacccca
    11161 ggtgaatatc accttacaat gactgtttta ggaaatgaaa atgaaaatgg aaaattttcg
    11221 agaaaaaaag gaaatactac gatcaactat accaatcaat gggtatttga aaaagatttt
    11281 acaatagacg gaaaagtagc caaggaattg aacacgacgg atgttacgat taaaaaagac
    11341 aataattgga tttatatctt agtagcttca cttttcttat t
//
LOCUS       DADVXU010000087        11245 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.091, whole genome shotgun sequence.
ACCESSION   DADVXU010000087 DADVXU010000000
VERSION     DADVXU010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.091"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(125..1612)
                     /locus_tag="KB953_002200"
     CDS             complement(125..1612)
                     /locus_tag="KB953_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867164.1"
                     /translation="MQVKKKIILSILLAIIAGLYSINYFRNVHTISTSGDIAFHFARV
                     KGLSSIFSGPINFTTFNHYGSGVNYFYPYLTFFPAVIFYWISNNLIVSYILYVWLLNV
                     CTIMLMFHYGLKFLKRIDAAFIFSCLYTFYGYRTIDIYHRSAIAEAIALTVIPIVMYY
                     AYALIYEKKPCAIPLAVSMSLLIYTHVLSTFMSVIAVGLLVLGGFVVSETRKKESIRL
                     IVELSKAVGMTILLTAYFWYPMFRQIMVQSINEPDKTTLQHQALNVSDSLIGAMNNDL
                     TTFTVGIIGIISLICPLFFFKGFNRKEKVIYLSAVVSWLLSTKFFPWSLLQNTPIQMI
                     QFPWRILGFQVLFGSLILSFVFMKSTSAKQRKVVNITAIVLLLLAVSAATKANYEHKV
                     VTQRDHLVMNQENLIRYTTLLPGGLYDYAPTEALQYKEHLQNHEVRIGKEWHTMPYQV
                     MDSKIVYTVHESKGQKITLPVFDYWGTVAKVNGQKREIDNRTSVD"
     gene            complement(2051..2920)
                     /locus_tag="KB953_002201"
     CDS             complement(2051..2920)
                     /locus_tag="KB953_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBB6867165.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(2940..4061)
                     /locus_tag="KB953_002202"
     CDS             complement(2940..4061)
                     /locus_tag="KB953_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HBB6867166.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(4063..4458)
                     /locus_tag="KB953_002203"
     CDS             complement(4063..4458)
                     /locus_tag="KB953_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBB6867167.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(4477..5283)
                     /locus_tag="KB953_002204"
     CDS             complement(4477..5283)
                     /locus_tag="KB953_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBB6867168.1"
                     /translation="MNKMSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALS
                     PILRKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKV
                     GTMGPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEF
                     GYKQGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQP
                     MLDKILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(5276..6064)
                     /locus_tag="KB953_002205"
     CDS             complement(5276..6064)
                     /locus_tag="KB953_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBB6867169.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(6078..6554)
                     /locus_tag="KB953_002206"
     CDS             complement(6078..6554)
                     /locus_tag="KB953_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBB6867170.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(6626..9283)
                     /locus_tag="KB953_002207"
     CDS             complement(6626..9283)
                     /locus_tag="KB953_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBB6867171.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKR
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNISKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDA
                     IYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFK
                     QSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
     gene            complement(9302..9706)
                     /locus_tag="KB953_002208"
     CDS             complement(9302..9706)
                     /locus_tag="KB953_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HBB6867172.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFLASHIYGKILSAKFE"
     gene            complement(9938..10195)
                     /locus_tag="KB953_002209"
     CDS             complement(9938..10195)
                     /locus_tag="KB953_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HBB6867173.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
     gene            complement(10320..10652)
                     /locus_tag="KB953_002210"
     CDS             complement(10320..10652)
                     /locus_tag="KB953_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867174.1"
                     /translation="MPLDLSTGRKIRFSEIQYEGIGFTLIDLKNSTFEIKEGLDLKNL
                     LCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDLLIRWQVITNFLMELKKY
                     SNTQTKFS"
BASE COUNT         3588 a         2226 c         1625 g         3806 t
ORIGIN      
        1 taacttcctg ataagaaaac taattaaaag aaagaggtaa ctacataaac taattaaaaa
       61 agctaacttc atagttggag gataaatgga agaaacttca atgattgttt ctccttgtgt
      121 tccttcaatc gacactagtc ctattatcaa tctctctttt ttgtccattt acttttgcga
      181 ctgttcccca ataatcaaat acaggaagcg ttattttttg tcctttagat tcatgtactg
      241 tatagactat tttcgaatcc atcacttgat aaggcatagt atgccattcc ttgccaattc
      301 ttacttcatg attctgcaaa tgttctttgt attgtaatgc ttccgtgggt gcatagtcat
      361 aaagtcctcc gggaaggagt gtcgtgtaac gaataagatt ttcttgattc attaccaagt
      421 gatctctctg agtgacaact ttatgttcat agtttgcttt tgttgcagca gatacagcca
      481 acagtaataa aacaattgcc gttatattaa ctacttttct ttgcttagct gatgtgctct
      541 tcatgaaaac gaacgataaa atcaacgagc caaacagtac ttgaaagcct aagattcgcc
      601 aaggaaattg aatcatttga atgggtgtat tttgtaataa agaccatgga aaaaatttgg
      661 tggataagag ccaagagact accgcactaa ggtaaatcac tttttctttg cgattaaatc
      721 ctttaaaaaa gaagagtgga cagatcaaag aaataatacc aatgattcct accgtaaagg
      781 ttgtcaggtc attattcatc gcaccaatca aactatccga gacatttaat gcttggtgtt
      841 gtaaggtcgt tttatctggc tcattaatcg attgaaccat gatttgtcgg aacatcggat
      901 accaaaagta tgctgttaaa agaatcgtca ttcctactgc ctttgacaac tccacaatca
      961 accggatgct ttcttttttt cttgtttcac ttactacaaa gccgcctaag actagtaagc
     1021 caacggctat tacactcata aaagtggaaa gaacatgtgt ataaatgagt aagctcatac
     1081 tgactgctaa tggaattgca catggctttt tctcataaat cagtgcgtaa gcataataca
     1141 taactatcgg tataaccgta agtgcaatcg cttcagcaat agcagaacga tgataaatat
     1201 caatcgtacg gtacccataa aatgtatata aacagctaaa tataaaagcc gcatcgattc
     1261 tttttaaaaa ttttagtcca taatgaaaca tcagcatgat agtacaaaca ttcaatagcc
     1321 aaacatataa gatataacta acgattaaat tattacttat ccaataaaaa atgactgccg
     1381 gaaaaaaggt caagtaggga taaaaataat tcactccgct cccataatga ttaaacgtcg
     1441 taaaattgat tggaccagaa aaaattgaag ataacccttt gactctagca aaatggaagg
     1501 caatatcccc cgatgtagaa atagtatgaa catttctaaa ataattgatt gaataaagtc
     1561 cagcaataat tgctaggaga atggacaata ttattttttt ctttacttgc attacttaat
     1621 tatatactcc tttcatggat tgctttatct agtataactt aaattttaaa taaatcgtta
     1681 tttattttta ctctcactta aaaaagcact aaaattataa gagagggagg taatggtctg
     1741 aacaccaaga attgttccaa catcgaattg cttaactaat gagttcaaag cttaattatg
     1801 tggctcttat atttaccgag cgtggattgc gatctcttta gcgaaacaaa catctttcat
     1861 aaaaaaaaca gccatatccg ctttcttgga tatgactatt tactactact ttactatctt
     1921 tcattcttct tgtctcactt atgataaggc gctgggtatc atgagtgaat gatattgctg
     1981 aatacaaata gtccgaccac attaagttga tcggacttca taagcatttt attgactaat
     2041 acttttttgc ttaagaattg attctaacat cttttgctaa agttaagcat cctatagttc
     2101 ctgcattatc tgctaactta ggtgtgacaa tgtattcttc gatatttggc gtatcgacat
     2161 agccgttaat tagttctaaa aaacgtcttt gtactttctt tttcagatgt tcttgttgca
     2221 tgacaccacc accaaaaatt ataatatctg gtgaaagcat cagggtcgta ttacaggcac
     2281 attgtgcaat ataatccgcc tcgatttccc agaacggatc ttctatagaa agatcttgtc
     2341 ccttggttcc cgtacgcatc tcgatagcgg gtccagatgc caacccttcg agacaatcat
     2401 gatgaaaagg acaagtacca gagaaagtat ctcctgaatg tctcttaact aacgtatgcc
     2461 ccatttctgg atggctaaac ccttcaacaa aaatcccatc ttgtatagct cctccaccaa
     2521 ttccagttcc aacagtgtaa taaacaacac tggataaacc ttttccatga cctgaaacat
     2581 actccccgta acaagcagca ttcacatcgg ttgtccaggc aattggaatt tcgaaatgct
     2641 tattcaacgt tccaacaaaa tcaaaatttt tccaggcagt ttttggagta gaggtgatat
     2701 gtccataggt ctttgattca cgatggatat caatcggtcc aaatgaccct actccaatgc
     2761 tttctaattg ttctttatag tgattaaaga aatcaattac caaactcatt gtttcattcg
     2821 gtgtagttgt aggaaaactc actctttcta aaacctttaa atcatcattg ccaatggcac
     2881 aaacaaattt agtcccacct gcttctattg agccatacat ttcttactcc accgtccttt
     2941 tatctaggaa ttcgttcttc cctgtctaat agtttttcga aaaactcatg tggttccttt
     3001 tggtaccaat aggcaacaga agaaatatcg tcttgacgct cataaagctt gatgtcatca
     3061 tttcctaggg tttggaaagt aacacgtaaa ttttctttga aactaatggg gtcgggtaaa
     3121 tgccagcggt acaacccatg catcggtaaa gcatcatcgc caaaaccatg aactggattt
     3181 ggattaggtt ttccagcact aaaataatcc cgggtgtgat ctcttttaga ttgaaaagga
     3241 tagcccataa atagcgtatt aaaattttga gcatatgccc tttctccaaa gttcttttta
     3301 tggaaagccc aagcgccgcc aaaataatct tctgctcctg tagatgttat tgtagggaaa
     3361 tcttcatctc catctatata aaacttaaat tctccttccc cccaccagta tctttccaat
     3421 gcgcacaaag caaagtaggt tcctacataa tacccagtgc ctttgattcc atcaataagg
     3481 gtgtaatctt ttccttttgt cgtgatgggt tcacggttcc atttagcatg aaaatacatc
     3541 gcatcatccg gtacttcagt caatgcataa ttaaatgtat aaaaaatgtg cttaataaac
     3601 tcaggatgtt cattcgttaa agtaatctta gctttttttc gaaatggcat ctcaaaatag
     3661 caattcatcc ctgatgttgg attcactacg attggtaagg aattcacttc gcatcttgtc
     3721 ccaaacccat tacaaaaaaa atctcctaat ggactttcta cagaaggagt ggattcatca
     3781 tcccaataaa tacgcaaaat taaatcacgt aagacgaaac tcccagcgat tgttttgtct
     3841 gtcactgtca tccaaaaatg acgtaaaatt ccagggcctt ccatttctgc taaagtaatt
     3901 gtctctccag catttatcgg aacagaaggt gacccttttc tcccaattcc tagatgactc
     3961 gctgtcattg ccgctttccc tttttcacca tttttatttt ctggtgaaat agttctactt
     4021 tcctctccct tgtggagcca tatattattt aaacaattca ttttattcgt cctctatcct
     4081 ttcttcactt ttttgattat tcatatagat gatttgttgt tgtgcctccg caataattgc
     4141 tccaatatct aaattatcat ttcctaactg caaagcgatt gacattgcca agggaagatt
     4201 cattcctgca attacgtgca ctctgtcaga aatataggta aaaaactttt gatttacact
     4261 tcctccagcc atgtcagtta aaacaattat ttcgtctgta gtatccacta actctttaat
     4321 ttgttcatct atatcatcaa tttcttcgac atatgcacat aagctactaa tttgattatg
     4381 catacctgtt aaaaactcta gtgtctcttt aaaaccctca gctagtattg aatggctagc
     4441 aatgatcaat cttctcataa ttcaaatcct tttcctttac gctaatattc caaatgcagc
     4501 cataaccatc gcaaaaacaa taacgagtag aatcaaaatg gtcatactga tttttttctt
     4561 actgagtaga taatatgctg cagcagtaat aattactgga attaatgttg gtaaaatttt
     4621 gtccaacatt ggttggattt tcatactaac ttcaccaaaa gtgaatttaa gtggcgttga
     4681 aacactgata actgaagcaa ttaatgatcc aactactgtt agccccaata ctgaagcagc
     4741 ttcagttaaa taattaatct tttcaccgta ttcagtaaca atcttcattc cctgcttata
     4801 accaaattca aagcctctca ttcggaaaaa gaagattacc atgattaaaa tgaaccataa
     4861 aaagattcct acaggattat tttctaaagc catataagcc gcaattgatc ccatgatggt
     4921 tgggatcatc gtcatcaaaa gagagtcccc aataccagca attggaccca tcgtaccaac
     4981 tttaaagtca ttaatcgcat cttttgcttc taatccttgc ttttcctcca ttgcaacaca
     5041 agcacctaaa ataatggctg ccagccaagg ctgtgtattg taatactgaa agtgattatt
     5101 taaagcttcg atgtattcat catcatcttt gtataatttt cttaaaatag gtgacaatgc
     5161 gtaagtcaca gatgcacctt gctgtgtttg ataattaaag gtacaaactg acatcaacca
     5221 acgatagttc gctttcctaa gatccttttt cgttacttga tatttttcgc tcattttatt
     5281 catcctcccc aataaaccca acatcagaag ctcccgcaga agcagtgatt tgtgcatctt
     5341 tttcagctct atgatacaca ttaaatgcta aagcgaatcc tatgatcgcc gctccaataa
     5401 taggtacctt caaaaaagcg gctaagaaaa atccaacaag taaataacta atgtatcgac
     5461 ctgtaggcat aaagcgtaac aacatcgcaa taccaacagc aggcaacatt tttccggcta
     5521 atgttagccc atccataaac caattaggta aaacatccaa aacgtaatta acagcagact
     5581 gtcctaaagt aacggcaata aatactggaa ttgctgcaga taaaccaaag aaaattggcc
     5641 ccaaccacaa aacactattc attttcttaa attctcgttt attcgcatat tcctgtgatt
     5701 tgcgaacaac aaaaccattt aagattttga caattacatc tagttgcacc cctaacatac
     5761 ctacaggtag tcctattgct aatccggctt ctactccaag ctggctacca aatgccgttg
     5821 caataattgc gcacgttcca tagtccggta cagatgagcc accaaaaccg gcaactccta
     5881 atgacatcaa ttgaactgta ccgccaacta tcaaacctgt ttttatatca cccatcaaaa
     5941 ttcctgtgaa taatccccaa aaaactgcgt aatttacaaa gatcatcgga atcccataat
     6001 aatcaacatg tttaataaat gctaatatag tcagcataat aatttgaaaa atagtaaaat
     6061 gcattatcct tactccttta tcctaatttt gattgaattt cctgtgcctt atccgccgga
     6121 acaaattgtg taacaatttc tacaccagaa tccaacaatt ttttatacgt gctgatattt
     6181 tcatctgatg cataggctct agaagatagc tgtaatgcat tatctttaac aacagtaccg
     6241 ccaacaatta gtcggtcaat tctaataccg ttatctacta aatgaaggat tgtttccggc
     6301 tctttcacga tgacaaatac tttttcctga tcgtatttcc cctttttaaa attcgcaatc
     6361 gctgtttctt cagtgataat tgatacagcc attccagcag gtctagctaa acgcatactt
     6421 gatttttgga tgtcgttatt tgcgacagaa tcatcgatca ccatcactct gtttgcttta
     6481 gacattggtg cccattgcgt gacaataatc ccatgcaata aacggttatc aactctcgac
     6541 ataacaatac tcataaatac ttcctccttt atctttcatt ttcttacaag caattaccat
     6601 gccaaaacaa aaagaacttg ataaatcaag ctctttttac ataatcataa acgtatttaa
     6661 cctcatgaat cgggatgtcg acactataat aatgttcaac gacactgaaa gattgcttaa
     6721 aatgtgtctg aaatttttga aattcctcac tcgtttcatt gaaataaatt tcatcttcca
     6781 aagtattttg ttttactaat cgttcaatta agcaactaat gtgaacatat aatccgaaac
     6841 acgtattatt atcaaatgaa attcctagat cattttgtaa gatgtatata gcatctgaaa
     6901 cccgttctaa taatttagtc ggatttaata tggtcaattg ctccattaaa ttcgttagac
     6961 taaaattctt cagaatatta ttgttcaaaa ctttcagttc attatcatta aagtgtctat
     7021 tcaattgatt ggagaattta cttatattca ttcctaaaat catatcttca attgaaaaga
     7081 attctacatc ttctagatgt ggatttagtg ttccaacaat aaaaagaata ttgttctctt
     7141 tacgaagctc cgataaatag ttttcattca ctaaagagta atagtcacac gtttctatga
     7201 caagatcagt atcccttgga aagctatctt caagtatcct ttttaattta aacgaagttc
     7261 ctaatccgct tgcacaagaa cagacaatca tatccttctt aatatttgat gattcataaa
     7321 tataatccac attttgattt tcttttactt gttcaagaat ctgtttaagc ggtaaatcta
     7381 ataatatctg atttgcaaca tcaatagcca ttcttgttga aacattgttt agtagtccga
     7441 attttttttg agcgtctagt tccaaattta gataaatttt ctccagtgaa cccatatcaa
     7501 caagtaaaat aatttcttca tatccggtac actcttgtaa gtatctatta attttgtctg
     7561 caatctcact acttgaagtc attagtggca tatcaatggc gtcgtatata tatctctcaa
     7621 cgatcctatt agcagtactt gctatacagg ttgccgttag atctccatga gccaaaatca
     7681 gtgctaaacg tttttgctga aattctttac tacacattaa cttaaataaa ataaccaaaa
     7741 catttttttg aatttttgtt aaataaatat cccctatttg aataaaacga ttgactaatt
     7801 gaacttctcg actacaccaa ctattaatat cattgatgaa ggaatataat ttatcgtccg
     7861 atgttaattg gatttgataa atccaagaat tcaaaaagat agacgttatt tgaagataat
     7921 aatagtcaaa ttctatttga aaatctctct ctatttcctt aacatactgt ctaaatcgaa
     7981 tctcttgata ggtgggatat gttaaagctg aattattttg gatatcaaca ttcttttgtc
     8041 taaaatatct atcatatttc tcaaaaagct gctgcttatc agtgtctttc ttccacgttt
     8101 gataaagata ctctataagt tcgttgatat taaaaacttc gtctactttt tccaaatcat
     8161 ccatttgaat atattttaat tcattctgtc ttttcataac cttcatgccg ttgaaattca
     8221 cccattgtgg taaatgatac atctgaatat aaagttcttc caaatcatct aagaaataaa
     8281 aggcattggc acaggtcaat tgaatgatat ttttcaaatt gccaacattt tcactaaagc
     8341 gatagtttaa taaaaaatct aataattgtt gagatatcag tattttcttt gctagtcttt
     8401 cttcttctga tttaaaaaat atcttgatta actgttttct ttcataaaga gaacgactgt
     8461 ctaatgttgg aagtgttagc tttacaggta ttcttctcaa caaagttgat aatagtttat
     8521 cctcaggtgg ttctgatgtt gcaaatataa tccagcaact tgatgtgtac cagttttcat
     8581 tatcacccat ccgatgaaat ttcctcgtat ccataaactg aaaaagtttc tcctgacatt
     8641 cattgctcaa tgcatgaatt tcatctaaaa ataagactcc attattagct aattctgcta
     8701 aaccctttct tccatcttcg gctcctgtat atgcaccttt tacatgccca aaaaaattgg
     8761 acaagaaaag ttctggatta ttggcatatt cactacaatt aactgaaatg aaagatgcct
     8821 tctcttctat ataattattg gccaacaaat ccttatacac ataatttgct aaatagcttt
     8881 ttccagttcc agtctcgcca tgaagtaaaa tgggtaagcc atttggcgga tagtagattg
     8941 ctgctttaat ttgagcaatt acatttcgca gacttccttg acttccgata accctttcta
     9001 ttccaaccct aaaagagcta tcataccatt tttccgcttc ctctgaagag ctaaaaattt
     9061 gttgatttgg aactttctcc ggtaaagaaa agaagattac tggacgagta ttgatttttc
     9121 caaattctcc atcttttacg aattcattca aatattgaga gacaatatta cgactaacat
     9181 ttaatttttc agatattttc tgtgctgtat tgttccccca tttattttta atctcagagt
     9241 tctctatgac ataattgaca atgcgttctt ttaattcttc catcacagca cctcctcgac
     9301 actattcgaa ttttgccgac agtatttttc cataaatatg acttgctaaa aaggctttgt
     9361 ttagatcatt caattggaac gttgcttgtt ttgcttgatc cgctaagacg ttgaaaagat
     9421 actctggcga aatcgcatac cccaaaatat caacgattgt tgcaatcaga tcttcttttg
     9481 attcttcatc tgaaagtaat tcacgataca acatcgtttg tttgcttact gtgtcatatt
     9541 cctctaagga ttcatctgct aaaagaacca cttgttccaa caaacggtcg gataaatatt
     9601 tatagaaaat cagccccaat aaataatttt tatattctga cgcatccatt ttactacgga
     9661 gattatctgc cgcactaaat aacttctgat ttaattctgc acccaaaaag atacactcct
     9721 atctcttatt taaagttgct atataccctc ttattttagc atactcagtt ttagaaaaag
     9781 caaaaaaata aagaaactta ttcttgataa caagctacgt gacgtatatc cctaacacaa
     9841 aaatagccat atcctttggt ttggatacgg ctatttgata ttactcattc aattatcaaa
     9901 tcacagtagg tgtgaatctc atcgggatcg gcaatatcta cttctgatta ggatcaaagg
     9961 tgattttctc aataaaggtg tgatacaaag ctttaacttg cttcttctca ctctggataa
    10021 gaaagcaatc caaactagtc aatatctgtt ggacatcttt gacttgaatc ggttcccctt
    10081 gttgttgaaa gatccctaag atttcattct ctcgaatttg taactgtctt cgtttttcag
    10141 tcaattcatt taaacgatct tttaacatcg gaaacaattc gggactatct tccaaagcag
    10201 cttcctgttt ttgctctaat agttagattt tgcgtaccat tttcttcttt tattttcctt
    10261 cgattgctga ttttaatata gtctaaagga aatttatatt tattgtattc tttataactt
    10321 caagagaact tcgtttgagt attactgtat ttttttaatt ccataagaaa attagttatt
    10381 acctgccacc taatcagcaa atctgaacct gagtcaatat aattaatatt attttccatc
    10441 tccaacattg taagtactgt tggtgttaat atagggactt tgtacaattg tatttttttg
    10501 attttatcat ctgcacaaag gagatttttt aaatctaatc cttcttttat ttcaaaagtt
    10561 gaatttttta aatctataag agtaaaacca attccttcat actgaatttc tgaaaatcga
    10621 atttttctcc cagttgatag atcaaggggc agaaaacctt ttattacatt gtgagtgtca
    10681 ccatcattta caatgtcatt aggaattttg attttagaat tctgtggagc acctattgga
    10741 agataataat tcattataat tttgtagtct tcatgaggaa ataaagtact tttaggattg
    10801 ctaagatatt ctcgtaccga atctaagaat ggcttatcca ttgaatcttc atccccattt
    10861 aaactaaaaa acattcctac aattgctttt gcaactctaa gtgaattcat tttctttatt
    10921 gaatgtgtta aatcagactt accattcttt aaacttgtat tgttataaag gaaattttta
    10981 ttgtcttctg caaatctaat atattcctca acatactcac taccaaaaaa actattacat
    11041 ttactacata acgtattgga atggcaacag tttttttgac aaattttata aggtgcagaa
    11101 cttctttccg tatgctattc cgattgtcct tgacaatgag cctcctcgga tgtgacgatc
    11161 ttcggcagga gctaacagtt aaagttagac tgcctcgcta gcgttagcac atccgagctc
    11221 attatcaagg attcgctgcg ccaat
//
LOCUS       DADVXU010000088        11234 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.092, whole genome shotgun sequence.
ACCESSION   DADVXU010000088 DADVXU010000000
VERSION     DADVXU010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.092"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            151..426
                     /gene="rpsP"
                     /locus_tag="KB953_002211"
     CDS             151..426
                     /gene="rpsP"
                     /locus_tag="KB953_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBB6867175.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            438..689
                     /locus_tag="KB953_002212"
     CDS             438..689
                     /locus_tag="KB953_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HBB6867176.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            814..1605
                     /locus_tag="KB953_002213"
     CDS             814..1605
                     /locus_tag="KB953_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867177.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            1775..2296
                     /gene="rimM"
                     /locus_tag="KB953_002214"
     CDS             1775..2296
                     /gene="rimM"
                     /locus_tag="KB953_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HBB6867178.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            2286..3047
                     /gene="trmD"
                     /locus_tag="KB953_002215"
     CDS             2286..3047
                     /gene="trmD"
                     /locus_tag="KB953_002215"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBB6867179.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            3183..3530
                     /gene="rplS"
                     /locus_tag="KB953_002216"
     CDS             3183..3530
                     /gene="rplS"
                     /locus_tag="KB953_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBB6867180.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            3903..4565
                     /locus_tag="KB953_002217"
     CDS             3903..4565
                     /locus_tag="KB953_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867181.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            4644..7871
                     /gene="fss3"
                     /locus_tag="KB953_002218"
     CDS             4644..7871
                     /gene="fss3"
                     /locus_tag="KB953_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HBB6867182.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITGAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            7985..8299
                     /locus_tag="KB953_002219"
     CDS             7985..8299
                     /locus_tag="KB953_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBB6867183.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            8312..8686
                     /locus_tag="KB953_002220"
     CDS             8312..8686
                     /locus_tag="KB953_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBB6867184.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            8696..9040
                     /locus_tag="KB953_002221"
     CDS             8696..9040
                     /locus_tag="KB953_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HBB6867185.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDVEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            9111..10463
                     /locus_tag="KB953_002222"
     CDS             9111..10463
                     /locus_tag="KB953_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HBB6867186.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            10523..>11234
                     /locus_tag="KB953_002223"
     CDS             10523..>11234
                     /locus_tag="KB953_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HBB6867187.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGML"
BASE COUNT         3834 a         1950 c         2420 g         3030 t
ORIGIN      
        1 acttattttt aacaaaaaag aagtctaaaa tcttctattt tctaaaaaaa atcgaattta
       61 atcaagctgt aaagaaaaaa agctttacag ggtaaaaaaa aactgttaca ctacatcttg
      121 tgaataaaac aatggaggtg gaaataaata atggcagtta aaatccgttt aaaacgtatg
      181 ggttctaaaa agagtccgtt ttaccgtatc gtcgtagctg attcacgttc tcctcgtgat
      241 ggacgtttca tcgaaactgt aggtacttac aatcctttga aagaccctgc agaagtagtt
      301 ttaaaagaag acttagttct tgactggtta tcaaaaggtg cacaaccttc tgacactgta
      361 cgtaacatcc tttcaaaaga aggcgttatg caaaaacacc acgaagctaa attctcaaag
      421 aaataaggtg accggatatg aaagatttaa gcgacttagt cttaacaatc gttcgtccgc
      481 tagtcactta tcctgatcaa gtcaagttag acgtcgtaga gtcgaaagac ttttacgagt
      541 acaatctaac agtggctcct gaagatattg gccgtatcat tggaaaacaa gggcgtgttg
      601 ctaaagcaat ccgcacaatc gtttacggtg ttcggatcaa tgcaccgaaa aaagtgcgtt
      661 tgaatatcat cgatgataag gaaaagtaaa taaacgtgct gtactcgaat tttcgagtgc
      721 agcttttttg ttttttttat taattgaaga aatgattatt tggcaaaaca agcaagaaag
      781 cgtatcctta taggaggagg gagtgaacag ccaatgagaa agaaaatgtt atttattcca
      841 tttgctctat tgttaagcgc ttgtagttcg gttactcaag aatcagacaa tacacatcta
      901 tcaagcagta tatctgaaac tgttaccagt ccagaaaaaa gtagtaccaa aacggcttcc
      961 accactgaac aaacaatggc tacctcaaat aatgagaaaa agactgcttt agaccagttg
     1021 aaagaacaac agccaaatgt tcccatgcct ctagatgtac cagtctcttc aggctatctc
     1081 aatatcgcag cgacacatac aaagcaaggt tattctattt tatattacag aacagatcgt
     1141 cccctcgggt tgaatgctga cgaattgaat caagaaacgc ctattgcaac ctatctatat
     1201 caatatgggt ttgcttctag tcaagaaaca atccaagttt tgcagccatt tgagatcgac
     1261 acaaatggtc agcaagtgga cttaggttct cgaatcacag ggtatcagca aggtgcagca
     1321 ggttcaagtt ttcttgaatg gcaagaagga aactggcgta ttcgaatccg tgggaataat
     1381 atagaaggcc aagatccctt actattagct aaagaaatcg tagcttattt ggaagaaaac
     1441 agtctaccag cacctgaaca atttggtaaa attactgttg atatgggtga tacgacgaat
     1501 cgagccgtag aagtaagctg gcaagaaccg aaaaatgcct atacgatcac tcatcaagat
     1561 cctatgtctg cgttgaagat ggcagtttct atgaagaggt tgtgaaatca gcgcgctgat
     1621 ctgagcccat catttaactc caagtcacag gaagtaatca aaaatagcac gccgcatttt
     1681 tgcataattt tggcttaata ccttaaaaga aaactagaat tgcctcttca cacatgctaa
     1741 aataaaacag acaaaaaaga aatggggata taagttgact gaatacttga atgtaggaaa
     1801 aatcgtaaac acacaaggta tcaaaggcga agtccgtgtt atctcaacaa cggatttccc
     1861 agaagaacgt tacaaaaaag gaacggtgct aacactattc caagacggaa aagcacctgt
     1921 ggaattaaca gtcaaaagtc atcgcaaaca taaaaatttt gacttattga gttttgaagg
     1981 acatccttct atcaatgatg tggaaaaata tcgggatggc atattaaaag tgtcaaaaga
     2041 caactctgta gatctagctg aaaatgaatt ctattaccat gagatcatcg gtctgcatgt
     2101 attagaagat ggaactgagt taggtaaagt aaaagaaatc ttgtcaccag gtgccaatga
     2161 tgtttgggtc gttcaacgtc cgaaaaaagc tgatatattg cttccatata tcgcttctgt
     2221 cgtcaaagaa gtcgatttag aagctggagt ggtccgtgta gaaattccag aaggactgat
     2281 tgacgatgaa gattgatgta ttgactttat ttccaagaat gtttgaaggt cctatggggg
     2341 aatccatcat cggtaaggct gtagataaag gattattaga tatcaatatc tctaacttcc
     2401 gcgattattc agataacaaa catcagacag tagatgatta tccatacggc ggtggagcag
     2461 gtatgctgct caaagtccag cccatctatg ataacatcca agccattgaa aaagctgcac
     2521 cggatgtcaa aaaaagagta attcttcttg acccagctgg taaacgattc gatcagaaaa
     2581 tggcagaaga attttcacaa gaagaacact tagtattcat ttgtggtcat tatgagggat
     2641 atgatgaacg tatccgcaca ttagtgacag atgaagtttc cttaggagat tatgtcctta
     2701 ctgggggaga attaggtgcg atggtcatga tcgatgcaac agttcggctt ttgccagatg
     2761 tgttagggaa tcagacttca gctcaaacgg attcttattc aaccggttta ttggaacatc
     2821 cccaatatac tcgcccggca gaatataaag ggatgaaagt accagaagta ttgacaaatg
     2881 gaaatcataa attgattgaa gaatggcaat taaaagaatc actaagaagg acctatcaac
     2941 gtcggcctga tcttttggaa acacttgaat ggacacctca aatgacgaaa tttttagaag
     3001 aaatcaagaa agaagaacaa gaaaaaacag cagatgaagc caagtgactt tttctaaaat
     3061 cttctttaca gtaggttgat aatatgttag tattttatgg tgagtgcaaa agcacttaac
     3121 tattacgata ttccgctgtg agaaaagctc ataagaatat ttggaataag gagaagaaaa
     3181 ccatgaatcc attaatcgaa gaattaacaa aagaacaatt acgttctgac atcccagcgt
     3241 tccgccctgg tgacactgtg cgtgttcacg caaaagttgt tgaaggtact cgtgaacgta
     3301 tccagttatt tgaaggtgtt gttatcaaac gccgcggtgc tggaatcagc gaaacttata
     3361 cagtacgtaa agtttctaac ggagtgggcg ttgaacgtac attcccattg catacaccac
     3421 gtgttgcaaa aatcgaagta gttcgctacg gtaaagtacg tcgtgcaaaa ttgtactacc
     3481 tacgtgcatt acacggaaaa gctgctcgta tcaaagaaat cagaagataa acacggaaaa
     3541 cttccttgga aatctgaaat ttgtgaaaag tccttgtata tcaagggctt tttctttttt
     3601 ctctatcaca gttttaagcc atttttggct gttttggacc atttttcaat catattccct
     3661 taaaaattgt cctagattgt ccgacattga ctatcagtgt cgtcaaaata gggcaaaatt
     3721 tagtagtcag cttgacgaca aatataatac gaaaaatacc aaaacattta ccgaatttag
     3781 caacttttga acccaagttt ctcaaagggt cagctattag caaaaaagac aaaacatgta
     3841 cttttctact aaaaagttga tgtgataggc ttctatctaa ataactatta ggagttttta
     3901 tcgtgaagca aaatgatgga cgtatcattt ggaaaaatca aagtgagtta aaactgattt
     3961 taacaattaa tgaattcatc gaaaaacacg gaattacatc tagccgtcaa taccagaaaa
     4021 agctatcaga aaatccaaat tctgcaccaa gtatgtggtt cattaacaaa aaatatggat
     4081 catgggagaa tttattgatt agtataggac gagaaaatac tgggtatgga aaatgggcta
     4141 gaatgtcaga acaagaatta ttggaaatag tagaagcctt tattaagtgc gagaagataa
     4201 cctctcaacg aatgtacgaa caaaaatcag tagggaagaa tattccttca cttagtacca
     4261 ttaaaaagat gttaggagat atacgtccac tttttaaaga aaaaaatgat ggatctcgat
     4321 ttactgattt cgaactgctg ttagaactaa aaaatgaaat aatccgatta aaactacagg
     4381 atgatttatc aatgacaaag tttcgaaaac tagttcaatc cccaaaacta ccatcagttg
     4441 atacaattat gaaaagaacg aacaaaaatt gggaggaatt gatggcagag attggatttg
     4501 attatcgaag aatcaaaatt tataagcaga gaaacaacct atccaagaca aagaaaacta
     4561 aatagtatca ctctaaatca gacgttcaaa attaaaattt ggacgtcttt tttattaccc
     4621 aaaaacgaaa ggagatttat tttatgaaaa agccaagatt taagaattgg cgtttgctcg
     4681 caacactgag tctattatgc caaacaattg gtggatcgct tggcccaacg attgcctttg
     4741 ctgatgaaat tactcatcca caaacggtaa ctgtggaatt ggatttggct caccagtatg
     4801 ctgtcgaagg tacatttagc gatggtcgtc ccatgtcaga agttaccgtt ccgcactatg
     4861 cagtttacaa tggtgtgaag caagatattt tctgtattga gccaggggtg ccgatttaca
     4921 atgagttcac tcctggttat gagaaaaatc cattgcctga tatgtcggag aaagcgaaat
     4981 tagtttccgt tttatggaag gaagctggta cggatgtgga tacacatatt gtggcccaga
     5041 agatgatttg gcaagaagtt aatggctaca cgcttcactc aattaaacgt tctgatggta
     5101 gtgcagtaaa tatcgcagcc attgagtcaa aaatcaatca agccattgcg gattatcaaa
     5161 agaaaccaag cttccataac tccacagtga aaaccgtgtt aggtcaatcg actactgtga
     5221 cagatacgaa cggcttgaat ttatcggagt ttgatgaagt tgtcgagaat atcgcaaata
     5281 ttgattatcg tgtgaacggc aatcaactag tgattactcc aaatgccaat tcaaaagaga
     5341 gtggtgaatt aaccctaaag aagtcagcta atacaggaac accggtagct tataaaatgg
     5401 ctggtcaaca aactttgatg gcgggggcaa ttgataagcc caatacctac acaatcaaaa
     5461 ttgatgtgga aactgagggt tctttgaaga ttaaaaaagt cgataaagaa acaggaaatg
     5521 ttgtaccggg aaccgttttc catttagact ttggaaaaac attacctgca aaagacgtga
     5581 ccaccgataa agaaggcatt gcgacattgg atgagattcc tcatggcgcg aaagtaacca
     5641 ttactgaaaa atcagtgcca gctccttata cgattgacac tacaccaatc accgcaacca
     5701 ttaaatctgg tgagactatt tctgtgactt caaaaaatac tcgcgaaaaa ggtcaaatca
     5761 ttttgaataa aatgggggtg gaaacaggaa ctgatctttg gaacgacaaa tactcattag
     5821 ccggaaatac gtttgccatt cgcaaagaca gtccaaccgg tgaaattgtc caagaaatga
     5881 ccacggatga aaaaggtcat gcggaaacac caaaagagat tgccaatgcg ttggaattgg
     5941 gaacctatta tgtgaccgaa accaaagcta gtaatggctt tgtgaatacc tttaaaccag
     6001 taaaagtcga attgaactat gtcaatcaaa ccgtggctct tgttaccagt aacgtaaaag
     6061 ggcaaaacca agaaatcact ggtgaaacca ctttgacaaa agaagacaaa gataccggta
     6121 atgaaactca aggtaaagct gagtttaaag gagctgaata tactctcttt actgcaaaag
     6181 atggtcaagc ggtcaaatgg agtgaggctt ttaaaccaga gttagtgaag ggaacgaaag
     6241 cttctgatga aacggtgact ttgactttag atgaaaagaa tcaagttgct gttaaacact
     6301 tagcgattaa tgagtattac tggcaagaaa ccaaagcacc tgaaggatat accttggatg
     6361 aaacaaagta ccctgtttcc atcaaaaagg tggatgataa cgaaaaaaat gccgtgatta
     6421 cccgagatgt tacggcaaaa gaacaagtca ttcgctttgg ctttgatttc tttaaatttg
     6481 ctggatcagc tgctgggact gccgaaactg gcttcaatga tctgaccttt aaagtatcgc
     6541 cattggaagg aaccagtgag attacaggtg ccgaagatga agcaattaca gcttataacg
     6601 agcaactagg ttttgatggc tatggcaagt ttgaaaatct tccttatggg gattatttac
     6661 ttgaagaagt agaggctcca gaaggatttc aaaaaattac gccattagaa atccgttcta
     6721 cttttaaaga aaataaagcg gattacacta agagtgaata catttttacg attaccgaaa
     6781 aaggacaaaa acaaccaatt aaaacggtga ccgttcctta cgagaaactg acgaataaag
     6841 cattttctgt tagcttgaat cgtttgatgc tctatgattt acctgaagaa gaagatagct
     6901 taacttctct tgggacttgg aaagacggag gcaaagaatt gacagacctt gattctaccg
     6961 agctagttga taaattgagc tataacttgc atgaaatcaa agaagactgg tatgtggtag
     7021 cgcaagccat tgatgtggaa gcgacaaaag ctgcccaaga aaaagatgaa aaagccaaac
     7081 cagtggtaat tgccgaaaca accgcaacgt tggcgaataa agagaaaaca ggaacttgga
     7141 aaattttgca taaattaacc gcagaacaag ttttggataa aaccatcgtc ttattcaact
     7201 atgtgtatga aaacaagaaa ccctatgaag caggtgatga tccagtagcg aaagatgcta
     7261 gcttgaacaa tcaagcccaa actgtcaatt gtacggtgga acgccatgtt tccatccaaa
     7321 caaaagccca cctagaagat ggttcgcaaa cctttactca tggggatgtg gtggatatgt
     7381 tcgatgatgt gtcgattacc catgatgtac tggatggctc aaaagaagct tttgaaacaa
     7441 ttctgtatgc attacttccg gataatacga ccaaagaaat ttggaaatct ggcaaaattg
     7501 attatgaagt gaatgacaaa gaattcacta aaaccgtact tgcggaaaaa gtggacacaa
     7561 gtaaatatcc agaaggtaca agttttacct tcaaagaaat taactatggc aaagacggaa
     7621 acatcaatgg gaaacacaat gaggatctaa aagaaaaaac tcaaacttta acacctaaaa
     7681 aagttccgac aacaccaagt gcaccagaac aaccggaaat accaactgtt ccaagtgact
     7741 ctcaagaatc tagtcctaca gtaaagacat tcccacaaac aggggagaaa aattctaatg
     7801 ttctgctgtt tattgggttt accttgatct ttgcgactgc tgggtattat ttctggaatc
     7861 gccgaaacta aggtgatgtg atgaaacaac taaataggaa aaaccaacgt gcgcgaccgc
     7921 ctcctaaaaa aagtggtcgc gctatttttg tatccaaaac taaaaatgaa aagaggaaaa
     7981 gaagatggaa ttgaaatttg ttgtgccaga tatggccgaa acctttggaa agataaggta
     8041 tgccggcgaa ggcgaagttt taactgaagg atatgggcgg aatactacag taattggtcg
     8101 tagttatcat ttgtattcca gtaagcaacg agccgatgat attgaagtgg tggtagctgc
     8161 ggaagccggc gaaaaggatt ttgatcaaga tcaaccacta aaagccgtga atccccattt
     8221 ggttgccaaa ggctatgaga ttgagaatcg tgggtttacc gactacgtgt tgtatgtcga
     8281 tgatttagtg aaagcatagg aggaaaagaa gatgagatta acagaaggta ttgttgtaga
     8341 ttcaggacta acgtttggga aattacgatt ttccgcatta cgccgagaag tacggaagca
     8401 aaatgaggat ggaacggtta gtaacgaggt aaaagaacgg acttataatt tgaaatcctc
     8461 tgcgcaaggt cgaatgatcc aagttagtat cccagcaaat gtacccttac gcgagtttgc
     8521 ctatgacgca gaggtagagt tggtcaatcc aatcatggat acggtcgcca attatgtttt
     8581 ccgagaagga actactgtca attggttcat taaggccgat gatttagtct tgaaacgaca
     8641 accagatcag ggaaattcta caaaccaaaa tgaaggaaag aaataggtga agcgtatgga
     8701 aattaatgtt gaacatattt tagattgtct cgatcaatat ggaaaaggtg agctaacgga
     8761 ggaacaactc actaaagcat taacctatga tgaaaaaatg tttctgatta tgcatcaagg
     8821 gttactggaa gtaggtaacg atgtcgaaga agattttgat gtactgaatt ggttagggga
     8881 agaagagtcc ttctttatgg ttatcgaagt aaatgaaagt ctatgtcgag aagccgaaac
     8941 cgtattggaa gaaattggtg tggaaatgcc ggatgccatt gaagtttttt taaagcaact
     9001 agtggaaacg aaacaactcc ctatagcagt aaatgactag cataactgct tgaaattgga
     9061 tcaatgaaat cctatgaagc ttttttcgat tggaggtgga aaccataaaa atgtacaaag
     9121 ggcatcgtat acgggcagga gatcagcact tggtttatca ctttgttctt ggctggctac
     9181 tcgctctgtt catcggttgg atgagcgtct tttattttca agagcttaga caatttgata
     9241 tttctaagct atcactttct actatagaaa tcgttcgttc tataaaagat ttaatctatt
     9301 tgctaggaag tctcgttctt tcaggaagca cgatgttgct ttatatacac ttttttcagg
     9361 atcattggcg cagtttgtgg catcgacaaa agctagcccg gatgattcta gaaaatcatt
     9421 ggtacgaagt aaaacaaact cagagtgaag gatttttcaa agacctcaat agtagtcgaa
     9481 ccaaagagac aatcagttac ttccccaaaa tctattaccg tatgaaggat ggtttacttt
     9541 ctattcgcgt tcaaatttca ctgggaaaat atcaagatca gcttttaaag ctggaaaaga
     9601 agttagaaag tggtctatat tgtgaactag tagaaaagga actcaaagac tcctatgtgg
     9661 agtacacttt actgtatgat ataattgcca atcgaattgg gatagacgaa gtagtggcag
     9721 aaagtggggc tttgcgattg atgaaaaatc aagtatgggc ttatgattct ttacctcata
     9781 tgttaatcgc tggcggcaca ggtggtggga agacttactt tttactcact atcatcgagg
     9841 ctttattaaa atctgatgcc gagctttttg ttcttgatcc caaaaatgcg gacttagccg
     9901 atttaggtac ggtaatgcct catgtctatt cccaaaagga ggaaatttct gcttgtgtgg
     9961 aagatttcta tgagcgcatg atgactcgta gcaaggcaat gaaagaaatg cccaactaca
    10021 aaacaggaga gaattatgcg tttcttggac ttccaccaaa ctttttaatc tttgatgaat
    10081 acgtggttta tatggaaatg ttaacgacga aggaaagtgc ggtgattttg aataagctca
    10141 aacaaatcgt gatgttaggt cgtcaatctg gtttctttct gattctagct tgtcaaagac
    10201 cggatgcgaa atatttagga gacgggattc gcgatcaatt taatttccgg gtggctttgg
    10261 gtcgtatgag tgaactggga tattccatga tgtttggcga ggttgataaa aaattcttta
    10321 tgaagcgtat caaaggtcga ggctatgtag atacgggagg gagtgtgatt agtgagtttt
    10381 atacgccact tgtaccaaaa ggatatgatt tcttaaggga aattagcgac attggtggat
    10441 taaacgttaa tacggaagga taaaataata gtatgtagcc taggagtgtc aagaatcctg
    10501 ggcttttttt acgaggtgat ttatgacatt tttagattta ttcgctggga ttggtggctt
    10561 ttgcttaggt atggaacaag cgggtcacca gtgtatcggc ttttgtgaaa ttgatgattt
    10621 tgctcgccag agttataagg caatccatga tacaagtaag gaggtggaaa tgcatgacat
    10681 cacaagtgta tcagacgagt ttattcaatc tctcggacca gtggatatcc tttgcggtgg
    10741 atttccgtgc caagcttttt caattgcggg aaagcggcaa ggattttccg atactcgagg
    10801 tactttattc tttgaaatcg ctcggttcgc cgctattctc caacctaagt ttttattcct
    10861 tgagaacgtc cggggattac tcaatcacga aggaggggct acgttcgaga cgatcctccg
    10921 aacgctggat ggattggggt atgatgtgga atggcaagtg cttaactcaa aggcctacgt
    10981 tccccaaaac cgtgagcgga tcttccttat cggacattct cgagacgcat gtaccgaaca
    11041 agtatttcct atcattggat cgtctccaac atctgatcaa aacatcagaa acttattaaa
    11101 tatcaaccct tccaatcgag gaatgggggg acaagtctat gggtcagatg acgtagcgcc
    11161 taccttgacc ggtgatgaag gaatcaaaat cgcattgccg gtagatgatg gaatttctgt
    11221 agcgggtatg ttac
//
LOCUS       DADVXU010000089        11138 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.093, whole genome shotgun sequence.
ACCESSION   DADVXU010000089 DADVXU010000000
VERSION     DADVXU010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11138
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.093"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            100..1359
                     /gene="lysA"
                     /locus_tag="KB953_002224"
     CDS             100..1359
                     /gene="lysA"
                     /locus_tag="KB953_002224"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBB6867188.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1346..2146
                     /gene="dapF"
                     /locus_tag="KB953_002225"
     CDS             1346..2146
                     /gene="dapF"
                     /locus_tag="KB953_002225"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBB6867189.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2149..3030
                     /locus_tag="KB953_002226"
     CDS             2149..3030
                     /locus_tag="KB953_002226"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBB6867190.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3027..3743
                     /locus_tag="KB953_002227"
     CDS             3027..3743
                     /locus_tag="KB953_002227"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBB6867191.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3749..4444
                     /gene="dapD"
                     /locus_tag="KB953_002228"
     CDS             3749..4444
                     /gene="dapD"
                     /locus_tag="KB953_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HBB6867192.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4460..5461
                     /locus_tag="KB953_002229"
     CDS             4460..5461
                     /locus_tag="KB953_002229"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBB6867193.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5458..6819
                     /locus_tag="KB953_002230"
     CDS             5458..6819
                     /locus_tag="KB953_002230"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBB6867194.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6779..7930
                     /locus_tag="KB953_002231"
     CDS             6779..7930
                     /locus_tag="KB953_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBB6867195.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7905..9074
                     /locus_tag="KB953_002232"
     CDS             7905..9074
                     /locus_tag="KB953_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBB6867196.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9249..10628
                     /locus_tag="KB953_002233"
     CDS             9249..10628
                     /locus_tag="KB953_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBB6867197.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10909..11138
                     /locus_tag="KB953_002234"
                     /pseudo
     CDS             10909..11138
                     /locus_tag="KB953_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3707 a         1946 c         2495 g         2990 t
ORIGIN      
        1 ttcagaaaat tttgtttaat attattccaa taaaattcaa atctttatca aatgaaattc
       61 caataaaaac ggaaaaaaag tgaaaagagg aatgaaaaaa tgacagaatt gataattggc
      121 ggagtcacag caagcacatt ggcagaaaag tataagacac ctctgtatgt gtatgatgaa
      181 gaaaagatga gacaaacgat gctgtcattc aagcgaggct ttgtatcgaa acgttttgat
      241 acaaaagtgc tttacgcctc taaagcattc caaacgattg agatgctgca tttagttcgt
      301 gaatatggat tcgggctaga tgttgtcagt ggtggggaaa tctataccgc tttgcaagct
      361 gattttccta gagatactat ctacttccat ggtaacaaca agacgccaga ggaactgatc
      421 tatgcacttg aaaatgattt gttgcatatt gttgccgata atttgatgga agttgaatta
      481 ttagcagaat taagccaacg ctatttaaaa aaaatctgta tcatgcttcg gttgaatgtc
      541 ggagtagaag cacatactca tgaatatatc gtcactgcgc atattgattc aaaattcggc
      601 atgagctatg aaagtaaaga gtgccaacaa gcccttcagt tgataaaaga aagcagctat
      661 ctagagttag aaggatttca tgctcatatc gggtcacaaa tttttgatgt tactgcttgg
      721 ttagctgaaa tcgacaaatt gacgaattac ttaaccgatt ttgaccaacc tctttccttg
      781 aatcttggcg gagggtttgg gattcgttat acacaaaatg atcagccgat cccagtcgaa
      841 gaggcactaa aaaaactgat tgaatatacg gaagaagctt tggaaaaacg aaaactgacc
      901 attcgtcaat tactgatcga accgggtaga agtattgttg gagaagctgg tacaacacta
      961 tacacgattg gtttcatcaa aaagacacca cataaaaaat attactttgt agatggcggg
     1021 atgacagata atatccgtcc agctctgtat caggcagaat acgattgtga tttagcagca
     1081 aaactgtcgg aagaaaaaac agagaaagtg acgattgcag gaaaaatgtg tgaatcaggc
     1141 gatgtggtaa ttaaagaagc ttatttgccg aaagcagaat cgggtgatct tttagctgtt
     1201 tattccacgg gagcttatgg atactcgatg agcagcaact acaatcgagc gacacgacca
     1261 gcagttgttt ttgtcaataa tggccaatca agactgattg ttcgcagaca agcattttct
     1321 gatctactaa ggggggaagt ttcctatgat ccttcataaa tataatggct gcggaaatga
     1381 ttttatctta ttagattacg cagaagacac agattatagt caattagccg cttctttatg
     1441 cacaaaagaa aaatatgata tagacggatt gatcgccgta aaaatcgatc cgttggaaat
     1501 gatctattac aacaaagatg gcagccgcgc gccgatgtgc ggaaatggca ttcgctgttt
     1561 tgctcgatac gtaaatgatc agaacatgat gaatgaacag gaattcaacg tagagacatt
     1621 agcaggcaca agaaaaatta cggtgttgga tccctcacct ttttattgca gcgtagatat
     1681 gggagtgcca gatgattctc cggaaaaaat caaagctaat caaacttatc caataaaaaa
     1741 ccaggaaata gagatcaatg gtgaaaaagt aacgatcact agtttatttt taggaacgat
     1801 tcataccgtt gtttttgttg aaaatgcgcg tgaggaagtc tggagaaaaa gaggtgaact
     1861 gatctgtcat catcctttgt tcaaagaaaa aaccaatgtc aactttgtag aagtagtcaa
     1921 tgaacaagaa ttgatcgtcc gtacctatga aagaggagta ggttggacac ttgcttgcgg
     1981 tacaggttgc tgtgcctctt atgtggttgc taaggaaact gggaaaattt cagcagatca
     2041 ggtagttgtt cacttggaac aaggagactt gatgatctct ggtgaggatg cgatacaaat
     2101 ggctggccca gcagttcatg aatggacaag aatattggag gaatgaagat gataaaaggt
     2161 tcgatcgtgg cgctgatcac gccgatgaat gaagagggat cagtagatta tgctggtttg
     2221 gaaaaattga tccaattcca tttagatgaa caaacagatg gattattagt tttaggtaca
     2281 acaggagaat cttctacgct cacacaatct gaggaggagc agatccttca actgacagta
     2341 aagaaagtgg ctggccgtgt gccggtgatt gcaggagctg gaacgaataa tacgaaagaa
     2401 acgattgaaa aagcgaagca ttttgcttct ttgggggcag acgcactttt ggtcatcacc
     2461 ccttactaca acaaaacaag tgatgcagga ctagctgctc attttacagc aattgcagaa
     2521 gcctcaccga ttccgcttat tttgtataat gttccttcta gaacaggcat gtcgattcca
     2581 atccatgtat tagtaaattt agcagaacat cctaatatca ttggtttgaa agaagcctca
     2641 ggagatatgg cttatgtgat ggatgccgct cggttgattg gagaagaatt cttcctttac
     2701 agtggaaatg atgatttgat cctaccagta atgagtgtag gaggaagcgg tgtaataagt
     2761 gtatgggcga atatccaacc taaaatagtc catgaattag taaaagatac gcaggacgga
     2821 agatggcagc aagcaaaaga aaagcaatta aatgcacttg aactgatcca tgccttgttc
     2881 agtgagacca atcctattcc tgtcaaagca gccatgtcat tattagactt accttccgga
     2941 cctctacgcc tgcctttagt ttctctttca aaagagaaaa agaaacaatt agctcagctt
     3001 ttgttaaaag aaaggacggt tgaaaaatga aaatcggact gattggcagt ggacagatgg
     3061 gaaatagagt cgaagaagtt gcacaagaaa gaggagatac ggtccttctt ttacggacgg
     3121 cacctaaaga agcaactacg cttgctttta cggaactgcc agaactgctt attgattttt
     3181 ctcatccaga taatttagag aaaatccttt cgtatagctt atcatatcaa cttccgttag
     3241 tcattggcac gacaggttat acacaatcac agttccaaga aataaaagaa catgccaaac
     3301 atgtacctgt gatgtatagt gctaattttt cgttcggcat catggtgatg aatcaactaa
     3361 tcaagcaagc agctgcaatg ttgcaaggct ggcagatcga actgattgaa aaacatcata
     3421 gtcgtaaaaa agatgcacca tctggtactg caaatatgct gcttcaaaca atccaagaag
     3481 tgcaaaagct gcagcccgta tacaactggc aagggaaaaa aagagaagaa aaccaaattg
     3541 gtgtacacag tattcgtgca ggttctttac ctggtgaaca tgatgtattg tttgctgcta
     3601 ctgatgaaat atttacgatc aaacatgaat ctttttctaa tcgaatcttt gctgcaggcg
     3661 cagtagaagc agcggactgg ttgaaagatc agtcaccagg ttattacaaa ttagaagatt
     3721 tattaatgga caaaggggtg taaaaaacgt ggaagctcaa gaaatcatcc gctacattca
     3781 aacggcatct aaaaagacgc ctgtgaaagt ctatctgaat gtaaccgagc caatcgaatt
     3841 taaagaaagt aaagtttttg gtgaaggaag cagcttcgtt gtttttggtg actatgagac
     3901 gattgctccg gttttagaag ctgaatcaga aaaaatcatt gaaatcgaaa tcgaaactgc
     3961 tgcaagatac agcgcagttc ctttgctaga tattaaaaaa attaatgctc gtatcgaacc
     4021 aggtgctatt atcagagatc aagtgtcaat cggcaacaat gccgtgatca tgatgggagc
     4081 gattatcaat attggtgcag taatcggtga aaacacgatg attgatatgg gtgctgtctt
     4141 aggtggacga gcgacagtgg gcaaaaactg ccatattggt gcaggagctg tcttagcagg
     4201 tgtgatcgaa ccagcatcgg ccaaaccggt tatcgtagaa gacggcgtac tggtaggtgc
     4261 gaatgcagtg attgttgaag gtgtccatat cggaaaagat gcagtcgtgg cagcaggagc
     4321 agtggtttta gaagatgtgg cagcagaaac tgttgtcggt ggaatacctg cacgtgtatt
     4381 aaaaataagt gatgatcaga caaaagaaaa tacagcattg attgctgcgt tacgcgaatt
     4441 atagaggaga tggagaaaaa tgcgcgaaat agatacctgt attgtggggg caaccggatt
     4501 agtcggccaa acgatgctca aagtgttaga agaatatgat tttccagtag gacgattgaa
     4561 attattagct tcaaaacgtt cagccggtaa agtttgtgta ttcaagaaaa agaaacacac
     4621 tgtacaggaa ttgacagaaa cttcatttga aggatatgat ttagcgctat tttctgcagg
     4681 tgcttctgta tccaaagagt ttgcgccaat cgcacgaagt cagggactat ttgtcataga
     4741 taattcctca gcatggcggg aagattcgga tattccatta atcgttccag aggtcaatct
     4801 ggaagactat acattgaatc ggctgattgc aaatccaaat tgttcaacca ttcaagcggt
     4861 acttccgctt aaagctttac aagaagcctt tggtatccag cgagtcaatt attcgaccta
     4921 tcaagcagtt tctggttccg ggcaaaaagg cattgaagat cttcatcgta cgcaaaatga
     4981 cgaggaacca gttttgtatc cttataatat tagccaaacg gtgattccgg aaatcgatca
     5041 gcctttggaa aatgggtata caaaagaaga acagaaaatg ataaatgaga cccgtaagat
     5101 tctgcatcag cctgctttgc cagtatcagc aacctgcgta cgtgtgccaa tcgaaaatgg
     5161 acatggagtc tctgtggcag ttcaactgca aaaagagttt acgacggaag aaattagaga
     5221 gtgtctcagc cagtttccag ggattcaatt agaagatgat ctagcagcac ataaatatcc
     5281 gacgagcatt cttgctagag gaacagatat ggtttatgtt gggaggatca gaaaagacac
     5341 ttctttagag aacggattgt tgttctatac gactgccgat aacatacgca aaggagccgc
     5401 tgcgaatgcc gtacagattg ctgcagcttt aatgcagaaa atagcggagg tggcaagatg
     5461 agtaatacgc tgaaagtagc gaaattcggc ggaacatcga tgtctcatga agagcaattc
     5521 aaaaaagtcc gtacaattgt tctttcagat cccaatcgcc aagtgatcgt tgtttctgct
     5581 ttaggcaaac gtacgtcaga tgatgacaaa gtaacagact tactttattt gatttatgct
     5641 taccttcagc atcatgttga ttggacaccc ctttggaaaa ggatttgtga tcgtttcatt
     5701 caagtaagag atgttcttgc cctgcagtta cctattgaag atgaactagc acgcatacag
     5761 aaacaactgg aagacgaaaa gataactgaa gattatttag taagtagagg tgaatatctg
     5821 actgctcaac tgatggctga gtatcttggt taccagttta ttgacgcaaa agacctgatt
     5881 ttctttgact atgatgggaa aatcgatcag gaaaaaacag cacaggaaat cacaaaagca
     5941 tttaaaagat atccgaaaat ggttgttcca gggttttacg gggcgaaccc tgcaggaaaa
     6001 gaaaaattat tgggccgtgg gggaagcgac attaccggcg cgatactggc gaagatgttg
     6061 caagcggaaa gttatgagaa ttggacggat gtttcaggaa tcatgatggc tgatccgcga
     6121 atcatcgatc atcctaaaat catcgaggaa atctctttta gagaactgag agagatggct
     6181 tatatggggg ctaacgttct tcatgaagca gctgttttcc cagttcaaga ggctgatatc
     6241 cccatccaaa taaaaaatac caatgatcct ttcgctcaag ggacaaaaat atcgaatcac
     6301 gaaatagaaa aggaaaatgg actgacagga atagctggac gcaaggattt tctttccatc
     6361 actttattca aacgtcatat gtccgatgaa atcgggttta tctggaaagc aatgagcatt
     6421 tttgctaaac atgagatatc aatcgaacat attccttcag ggatcgacaa tatcggagtg
     6481 atcgtctcag cagaagcgat tgccgataag ttatttctca tcacgaaaga attaaaagaa
     6541 acacttggcg tggaagaaat cgtcgtgatc gaagacttag ctttgatctc tgtcgttggg
     6601 ggtccgcata aagaattgat tggattatct gggaaagtgt tatcgatatt gaacaaatta
     6661 gaaattcgca catcgatctt atcacaagga gcacaggagt tgaatctgat catcggtgta
     6721 ccgaataatc aatatgaaac agtagtgaag ggtatctatg aagggatggt gaatacggat
     6781 gctgaaagaa tatcgaatgg cactccatca aattcctgaa atcgggtttc atgaattcaa
     6841 gacaaaaaaa tatttatatg aacaagtaaa aaacagcgag gccgttatcc atgaaatcgg
     6901 cgaaacagga ctactccttt attttgataa tcagcaagaa gaaacgattg cttttcgaac
     6961 agatatcgat gcactgccag tcaaagaaga aacgaatctc tcttttgctt ctaagcatga
     7021 aggttatatg catgcatgtg gtcatgacgg acatatggcg atgcttttag ggttgactga
     7081 ctttataact aaacacattg aggatcttat ttataatgta gtactgatct atcagccatc
     7141 agaagaaatt tccggtggcg cacaaagcgt tattcagtca ggattgttgg ataaatatga
     7201 agtacaagca atatttggct ttcatctatg gcctggacta gctaagggag aagttttctc
     7261 tcgaccagga cctttgatgg cacaaagcag tgaaacagac attatcatta aaggccagtc
     7321 tgcacatatt gcttctagtg aaaaaggaat cgatagtctg gaggcagctg tacgattttt
     7381 gaagaatgtt tatgattttg accggcaatt accggcagag tggaatcatc taatgaaatt
     7441 tggccaaatc caaggtggaa cgatccgaaa tgttttagct ggagaagtta ttgtatccgg
     7501 aagtatccgt tcttacagtt cagatatgca aaattatttg aagaggggat tagaccattt
     7561 agcagaacag tttcactcta cagctactat ttcttttcgc tataatgacg gctatccagc
     7621 tgtaatcaac catagaggct tatatgcagc actgttagat tcaaacaaac tacatgaatt
     7681 agatgctcca gttcttcaag ccgaagattt tggcatatat acgcaacaat atccatgtgt
     7741 atttttcttt ttaggtgtcg gtgatacccc tgctttacat gatccgacgt tcgattttga
     7801 tatggatgtt ttagaaaatg gtgtagaatg gtacaaaacg attcttttta cagaaaatct
     7861 tcttgtaaaa atcgatagca gttcaaaaga ggagcgagta aataatgatg aatttatcca
     7921 atcgatttag tcctaggctt tctagaatag aagtatcaaa aatacgacaa ttcgatcagc
     7981 aaatatcgtc gataccagat gtgatcaaac tcaccctggg agaaccggat tttccaactc
     8041 ctgaacatgt caagcaagca ggaatagcag cgattgaaga agacttttct cactataccg
     8101 gaatgagagg attagaggaa ctgcgggaag cggcttgtat atttcagcaa cagcgttatg
     8161 gattaactta tgatccgcaa acggaagttt tgacgacagt tggtgcaacg gaagcgattg
     8221 cctctgcgtt gttatctgtt ttggaagaag gagacaaggt tcttattcca gcgcctgctt
     8281 attcaggata tcagccatta gtggaactag ctggtgctga attgatcccg atagatactt
     8341 ctgacacagg gtttgtctgt cagccggaac aatttgaacg agcatttgaa caatatggat
     8401 cagctgtcaa agcagtcatt ttaaactatc ctaacaatcc tactgggacg acattgtctg
     8461 caaaacaatt gaaagcaatc gctgaggttt taaaaaaata tccggtattt gttataagtg
     8521 atgaagtata tgctgaattg acttatagtg gaacgcatat gtccatagct acttatcttc
     8581 cagaacaaac aatcgtgata agcgggttat ccaagtcaca tgcgatgact ggttggcgag
     8641 tgggctttat ttttgctcaa aaagccctta ttgacgaact aattaaagtc catcaatatc
     8701 tggtgactgc cgctacaacg atgtcgcaaa aagctgcggc tgaagcgcta ataaatgggg
     8761 tgaaagatag cgagaatatg aaagagcaat atcaaacacg aagagactat ctggtccagc
     8821 aattggcgcc tttaggattt gaagtgaccc agccaaacgg tgcattttac ttgttttgca
     8881 aactcccagc tgccattcaa actgactctt ggcagttttg tgtagaatta gcggaacagg
     8941 caaaagttgc gtgtatccca ggaattgcat ttggcccaga aggagaaggt tatatccgga
     9001 tttcttatgc tagcagcatg gaaaaattac atgaggcctg caaacggatc aaagactttt
     9061 taacagaaca gtgattcata gattttatca aaaaaacaat cgttaatttt gcggtatatt
     9121 ctgcaaggag acgattgttt tttaaattta gttcaaatga acgcgtttct tttgattcat
     9181 aactaatgta atgttaagtt taaattgttt agaatgattt taaaagaata ttcaaggagg
     9241 tttcatcaat ggaaaaaatc aaagttgtca ttgtaggtgc atctcatgga gggcatcaat
     9301 cgatcttgga actattgaca cgatacggtg aaaatgtaga tatcacgcta tttgaagcag
     9361 gagactatat ttccttcatg tcatgtggta tggagctgta tttagaagac aaggtaacag
     9421 atgtaaatga tgtacgtaat ttcagaccag aaaactttcc gcaatccaat gttcatattt
     9481 taaataatca cgaagtaaaa acgatcgatg ccgataaaaa aacagtaacg gttactagcg
     9541 catcagataa cgaaacaaaa gaatatcctt atgacaaatt gattttgagt tctggtgtca
     9601 aaccaaacaa tctgccagta ccaggagcag atctagaaaa tgtctacttg atgcgtggat
     9661 acaattgggc aaccaaaatc aaagaaaaat taaatgatcc ggctattaaa aaaatcgctg
     9721 tcgtaggtgc gggttacatt gggatcgaag cagcagaagc aagccgaaaa gctgggaaag
     9781 aagtcgtatt gcttgatgtg atcgatagac ctttaggcac ttatttagat gctgagatga
     9841 cagatatttt agaacaacac ctaaatgaaa acggtattga agttttgaca aatgcaaaaa
     9901 tcgaagcttt tactggtaac ggaaaagttg aggcaatcaa aacaagtgaa aaagaaatcc
     9961 cagcagatct agtcattcaa gctgccggtg tgaaggcaaa tacagaatgg ttaaaaggga
    10021 tcgtcgactt agatgaacgt ggctggatcc aaacaaatga atacttgcaa acgaatcttc
    10081 cagatgtgta tgcagtagga gatgctacac ttgcttattc tatcccagca cgcaaaaaaa
    10141 tgccgatcgc tttagcgact gttgcacgta gagaagcacg ttatgtagta aaacatcttt
    10201 ttgaagaagt accaaacaaa ccgttcggcg gagtcgtagg ttcctctgca ttaagtgtct
    10261 ttgactatca ttttgcagca agcggattga atagctttac agcagcaaaa gcaggagtag
    10321 atgtacgagt gtcttattat gaagatacgt tacgaccaaa atatgtacca gaagaacaag
    10381 gaaatccaaa agtgtccatc caattattct cggatccaat gacccatcaa ttgctaggag
    10441 gagcagtatt atctacctat gatgttacag gtcaaggaaa tgtattggca ctagcgatcc
    10501 aaaacaaact gactttagaa gaactagcag aagctgactt cttcttccag ccaggctttg
    10561 accgacaatg gagcatgttg aatctagccg ctcaacaagc gttaggagaa gaaccatttg
    10621 tagaataaga aaacgaaata tttgaaaaga ggttgtgaaa aagctgcctt gcatcaagta
    10681 ataagaacag ccaagtaaaa acagcttctt atgtttttaa cttggctgga cttattaccg
    10741 cagatgcaag cttttgaaca ccgtttattt tcaaaaaagg gactgtgaca cgatttgtgt
    10801 accgaccccc aaaagttaga ccaaaaaatc taacttttgg aggtcggtat tttttatggt
    10861 taaatatact tttgaactta aattaaagat cgttcatgat tatttggatg gcaaaggcga
    10921 ttggaccgta aaaatccaga tcaagagatt gaagcggaaa tgcttaaaat tcgggagaag
    10981 cacaaggatt atggctgttt acggatgacc aatgaactaa gaaaccgcgg tttctctatc
    11041 aataaaaaga aagttcagcg cttgatcaag aagctaggaa taaaagtaac ggcatacacg
    11101 cgtaaatcac gtcgctacaa ttcttatcga ggaaaagt
//
LOCUS       DADVXU010000090        11020 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.094, whole genome shotgun sequence.
ACCESSION   DADVXU010000090 DADVXU010000000
VERSION     DADVXU010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.094"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            138..1769
                     /locus_tag="KB953_002235"
     CDS             138..1769
                     /locus_tag="KB953_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBB6867198.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            1772..2851
                     /gene="uxuA"
                     /locus_tag="KB953_002236"
     CDS             1772..2851
                     /gene="uxuA"
                     /locus_tag="KB953_002236"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBB6867199.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            2925..4352
                     /gene="uxaC"
                     /locus_tag="KB953_002237"
     CDS             2925..4352
                     /gene="uxaC"
                     /locus_tag="KB953_002237"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBB6867200.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            4436..5083
                     /locus_tag="KB953_002238"
     CDS             4436..5083
                     /locus_tag="KB953_002238"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HBB6867201.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            5203..6579
                     /locus_tag="KB953_002239"
     CDS             5203..6579
                     /locus_tag="KB953_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB6867202.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            6589..7383
                     /locus_tag="KB953_002240"
     CDS             6589..7383
                     /locus_tag="KB953_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBB6867203.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            7396..9162
                     /locus_tag="KB953_002241"
     CDS             7396..9162
                     /locus_tag="KB953_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HBB6867204.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            9185..10993
                     /gene="uidA"
                     /locus_tag="KB953_002242"
     CDS             9185..10993
                     /gene="uidA"
                     /locus_tag="KB953_002242"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HBB6867205.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
BASE COUNT         3868 a         1414 c         2250 g         3488 t
ORIGIN      
        1 ttctgaataa aaaatacaat cattctgata tggtagaaaa aagcaagttt attccctttt
       61 tatattgaca acggttacaa tgggttttaa aatataaaat gtatcaaaac taacatatca
      121 gtttgaggag gttaattatg gtttcgatta aaaattggca aaattctatc gaagaattca
      181 atgaaatgca gattctactg ccgtgttttg atgtcaaaga agttaagcaa gaaggccagc
      241 aacatccagt atggcttcat tttggtggag gaaatcttta tcgaggattt catgcagaaa
      301 ttgctcaatg cttagcagat caaaatgaat tagaaacagg agtagttgtg tgtgaaacat
      361 ttgatgaaga agtagtggaa aaagcttatc atgactataa caatgacatt ttggaaattg
      421 ttatgagtgg agatggtgaa ctcaaaaaaa gacttttggc agtaacggct gatagtttat
      481 attgcaatcc gaagaatata gcggactatc aaagagtggt taaatatttt gaagatccta
      541 aattacagtt tacaacgttt actattactg aaaaaggtta cagtttaaaa gattctaaag
      601 ggcaattctt ttcggtagtc aaagctgata tcaaatctgg acctagcaat cctcaacata
      661 cgatgagtat tgtaacatcc ttgttatata gcagatttaa tgctggagag ttgccgattg
      721 cgatggtttc tactgataat ttctcacaga atggtcaacg ttttcaagat agtattttta
      781 cgattgcaga agcttggcaa aaagaaggat atgtttctga aaaatttatt gcttatttaa
      841 aggataaaga aaaagtgact tttccttgga gtatgattga tcgaattacg ccaaatcctt
      901 cagagaatgt aaaggaagat ctattgatga tggggctcga ggatttagat atcattcaca
      961 caaaaaaaca taccaatatt gctgctttca ctaatactga ggtagttcat tatttagtta
     1021 ttgaagatag ttttccaaat ggccggccta aattggaaaa ggctggtgtg attatgacag
     1081 atagagagac ggttgataaa gcagacatca tgaaggttac gacaagcttg aatccacttc
     1141 atactgcttt ggcagtaaca ggttgtctgc taggatatca atcaatttca gcagagatgc
     1201 aggatgatga tttggttggt ttaatcaaag gaataggtta tttagaaggt ttaccagtag
     1261 ttgaaaatcc aggcattatt gatccaaaac aatttattga tgaagtaatt aataaacgtt
     1321 tgcctaatcc ctatattcca gatacaccac aacgaattgc aacagatact tctcaaaaag
     1381 tagcgattcg ctatggtgaa acaattaaaa agtatgtcga aaaagacgga aatgcagaaa
     1441 atctaacatt tattcctatc gctattgctg cttggttacg ttatttactg gcagtcgatg
     1501 atcaagggga aaattttgtg ccaagtcctg atccgttatt ggtagagttg caggataaat
     1561 tatcagtatt gaaattaggt gaacaagatc cttcaaagat tcattgtgcg gtacaggaaa
     1621 ttttagaaaa taaagaaatc tttggtttag atttatatca agttggggta ggaaagaaaa
     1681 ctgaaaaaat cttttgcgaa atgctagtag gtcctcaagc agttcgtaaa acaatcaata
     1741 aatatataac agaagttgga ggaaagtaac tatggaaatg acttttagat ggtatggggc
     1801 aaatgaagaa aaaatctcac tacaagaaat tcgtcagatc ccagcaatga aaggaattgt
     1861 tggaacatta atggatatac cagccggaga ggtttggccg agagaacgaa ttaaatcttt
     1921 aaaagatgaa gtcgaagcca atggacttac cttgaaagtt attgagagtg ttaatgttca
     1981 tgatgatatc aaaattggtt taccaacacg agatcaatac attgagaatt ataaacagac
     2041 gattcgcaat ttggcagaat ttgggattga ggttatctgt tataatttta tgccagtatt
     2101 tgattgggta aaatcggatt tggattatcg tttagatgat gggtcttcta cgttagccta
     2161 tatttcaaaa aatatcccta ctgatccgga ggaaattatt agagaagtag aaaattctac
     2221 taataatttt gaattgccgg gttgggaacc tcatcggtta tcacaaatta agcaattatt
     2281 agaagcctat aaagaagttg atgaagagaa attacgagag aactttgcat attttctcaa
     2341 agcaattatc ccgacatgtg aagaagttgg gattaaaatg gcagttcacc cagatgatcc
     2401 accatattcc attttcggat tacctcgtat tgttaaaaat cgtgacgatt tagattggtt
     2461 gtgtaatgta gtggattctc cttctaatgg tattacatta tgtactggaa gtattgcaga
     2521 acgaccagat aatgatgtct atgaaattct atctgaattt tgtcaacggg atcggattcc
     2581 ttttgcacat gttcgtaata tcaaatattt aagtgacaat ggactcgatt tttacgaagc
     2641 gccgcataaa tcagaatatg gttctctaga tatgtataag atattaaaaa caatgtacga
     2701 taatggattt aatggctata ttcgtccaga tcatggtcgg atgatttggg gagaaaccgg
     2761 tagacctggt tatggattat atgaccgagc tctaggtgca gtttatttaa atggtatttg
     2821 ggaagcgctt gaaaaggggg cgagtaaata attatataag ggagtttatt tagaggaaaa
     2881 attttctctt ctaaaaacta acggaaggaa aggagtgttt aaatatgttt ttagataaga
     2941 actttttatt agagaatgaa atagctaaaa gactttttca taaatttgca aaacaccaac
     3001 caattatcga ctatcattgt catttagacc cagcatattt gctagaaaat aagcaataca
     3061 agaatttgac tcaggtatgg ttaaatgata aaggagctgg agatcattat aaatggcgat
     3121 tgatgcgagc taatggtata gaagaagagt tgatcagtgg tccaggtgat gattatgaaa
     3181 aatttttagc ttttgtaaaa acagtggaga aggcgccagg taatcctatt tttgaatggt
     3241 ctcatttgga attacaacgt tactttgata ttgatttgac aatttctgag aagaatgcac
     3301 cagaaatttg gcgacgtgct aacgaaaaaa ttcaaacgga ttcctatcgc ccgaaagaat
     3361 tgttggagat gatggatgtg aaagccatct gtacaacgga tgatcctaca gatgacttgt
     3421 ggtatcatac tgagctttct aaacaggata attctttcaa ggtattacct actttccgtc
     3481 cagataaggt ctggagtatc aaatcatcag gcttttcgaa ctatatttta aaacttagtc
     3541 aaacagcggc tataccaatc aaagacttta atgggttgaa gcaagcattg agccaacgag
     3601 tggatcattt tcatcaaatt ggctgtcgat tagcagatca cggtttgaat acttattttt
     3661 atgaaatagc agatgaaaaa atactcgaca atctgttaat aaaagcttta gcaggaaaaa
     3721 cggatttcca tttatcagaa attgcacaat ttgttacagg tattcaatta catttgatgt
     3781 ctgagtatac aagatatggt tggacgatgc aaatgcattt aaatgcttta agaaataatt
     3841 caagtatatt aaaggaacaa attggagtag atgtcggtgg tgattcgatg ggggatcaag
     3901 ctagtttgac acaaaatgtt gtagcattgt tcgataccgc tgaaaagagt gggtgcttac
     3961 caaaaacaat tttatattca ttaaatccaa ctgattggct tccactagca acagcaatgc
     4021 aaagcttcca agggggtatg attcaaaaaa tccaattggg atgtgcttgg tggttcaatg
     4081 atacagctga aggaatgcgt caacagttga ctacgatggc acaacaaagt ttgttgtcga
     4141 attttacagg tatgttgact gattctcgaa gttttctaag ctatcctcgc catgagtatt
     4201 ttcgtcgagt attatgtcaa ttactaagcg agtgggttaa agcaggccga attccagatg
     4261 atgaaatgta tcttagtcag atagtggaga atatttgtta tcgtaatgcg tataattatt
     4321 ttaatttttt tgaaatcgga agtagaaaat aatacttgaa ataaaagctt aaatttattc
     4381 tttgtaagtt agtacagcta gacaaagtat gtatccataa aggaaggaga tagatatgaa
     4441 aaaagtagaa attttaagta agttagaagc ggctggagtc attgcggttg tacgtgggaa
     4501 aaataaggat gaggccatta aagcaataaa ttcaattatt gcgggtggaa ttaaagggat
     4561 tgaactaact tttacagttc ctaatgctga agaaataatc aaagaaattg ctaaaaaata
     4621 tgctgatcaa aaagatgtaa taatcggagc cggtacggtt ttagatgcgt ttactgctcg
     4681 tatcgcaatt atggctgggg cagaatatat cgttagtcca tcttttgatc gacaaacagc
     4741 tgagatttgt aatttatatc aaattcctta tttaccaggt tgtatgacaa ttactgagat
     4801 aaaggaggca cttactgctg gcgtagatat cattaaacta ttcccaggtt ctgcttttgg
     4861 tccatccatt gtaaaagcct atcaagctcc attaccacac gtaaatataa tgcctactgg
     4921 cggagtaagt atagagaact tagaagattg gtttgaagta ggagttgttg cagttggtgt
     4981 aggtgggaat ttattggctt ctgttgaaaa aggtgatttt gaagaagtaa gtcgagtagc
     5041 tagtaaatat atagataagt tttatgagat aaagaattat taggagaaaa aacaaataat
     5101 actctgaaat gtaaaaaata aatactgact ctttacgctt cagagtatag tacgtacttt
     5161 catcagaatg aaaacgctta acaacagaag ggagagaatt gtatggaaag aaaatttggt
     5221 ataagagaca aaataggata tatgtttgga gattttggaa acgattttac tttcattttt
     5281 gctagttctt ttttaatgat cttttataca aaagtattgg ggataagcgg agctgccgtt
     5341 ggaacattat ttttagtagc aagatttatc gatgcagtta ctgatgtaac tatggggcgt
     5401 attgttgata gatcgaaagc aactaagcag ggtaagttta aaccgtggat tttaagaatg
     5461 agtggtccag tcgctttagc aagtttttta atgtatcaaa cttctatgat ttcagcttct
     5521 atgagcgtta aggtagtata catgtatgtt acgtatatat tatgggggag tattttttac
     5581 acatctataa atattcctta tggatcaatg gcatcagcta ttacttctat tacagaagaa
     5641 agaacaatgc tatcgacatt ccgaaatgtt ggagctactt tagcaagttt ggtcattggt
     5701 gtattaacac cattatttat ttatacaaga gatgattcag gcgctcaaat agttcgtggt
     5761 ggatcaactt ttacaattgt tgcgggaata ttttcgatat gtgcacttat ttgttattta
     5821 atatgttata aactaactac tgaacgagta agaattgacc aatctcaata caaagtttca
     5881 ttgttaaaat ctttaaaagg gttagttaaa aatagggcat tactttctat aattggtgcg
     5941 gctattttct tactccttgc tcaattatta attatgagta tgaataatta tgtatttcct
     6001 gattactata atgatgcaca gggaatagtt ttaatgaatt ttatcaatcc tattttggtt
     6061 ttaatagttg tttcgccttt aactttgttt ttaagtaaaa aatatggaaa aaaagaactg
     6121 gcatcagtag gaatgttctt ttcagcaata gtatatttga tcttattttt gataaaagct
     6181 agtaatatgt atgtattctt aattcttagt tcggttggct atatgggttt gggtgtgttt
     6241 aatactgtta tttgggcaaa tattactgat gttattgatg atcaagaagt aaagtctgat
     6301 caaagagaag atgggaccgt atatgcggta tattcttttg ctagaaaaat aggtcaagct
     6361 ctagctggag gcgtcggagg ttggactctt tctattatag gctatgactc tttagcaaat
     6421 gtacaaacgg gagatgtttt agaaaagtta tataacgctt caacattaat tccatcaatt
     6481 tgttttctga ttgtaggatt aattttattt ttcctttatc cattatctaa agataaagtc
     6541 ttagaaaatt gcgagctttt aaaacaaaaa cataaatagg agagaaatat gaataattgg
     6601 atattttgta ttgattcaga tggctgtgtt atggatacga tgacttataa acacaatctc
     6661 tttttcggac caattgctgc agagattttt gatgttcatc atagagaaaa attcctacag
     6721 gattggaata atattaattt atattcagag actcgtggaa taaacagatt tttaggttta
     6781 gttaaaacat tagaaaaaaa tgatgtaaca tcgattaatc gattaaagca ctgggtatct
     6841 ctaacaaaag aattgtctaa tccatctttg aagagggaaa tagaatattc tcagtcagag
     6901 gatttattaa aagctttaaa atggtcggaa gaagtcaatc aacagattag tcaagcaaaa
     6961 ggattagata aacccttttt aagtgctaga gatactataa aagctctgaa aaaatatggg
     7021 aagataatta ttgtaagctc agctaataag gaagctgttc aagaagaatg ggaaagacat
     7081 gaattattat cattagttga tgaactgtgt tgtcaagata aaggaaaaaa agaagatatt
     7141 attcgaagtg taattgaaaa tggttgtgat ttggataaga tattaatgat tggtgattct
     7201 cctggtgatc tagaagctgc aaataaaaat aaagttttct tttatcctat tttggtcaat
     7261 aaagaaaaag aatcatggga aaacttaaga actgatgttt tatttaaatg tttatcggga
     7321 gattatgtag atattgaaca aaaatatata gatacttttt ggaaaaatct tactaataag
     7381 tagaggagaa agaaaatgac tataaaagta gatttacgaa aaaaaccata taatttagat
     7441 gaagatgcta tttcttggat tgaagagacg attgattcta tgactttgga agaaaaaata
     7501 ggtcaattgt ttgttaatat gggatctagt cgtgataaaa attacttgag taatatggtc
     7561 aatcagtacc atattggagc tgtacgatat aatccaggaa ctgctgatga ggtatatgat
     7621 caaaactata tcttacaaac agaaagtaaa attcctctat taattgctgc taatacggaa
     7681 gcaggtggta acggagcttg tacagatggt actgaagttg gtgtagaagt aaaaattggt
     7741 gcaacaaatg atgcgaaata tgcttatgaa ttagggagag tttctgggat tgaagcatcg
     7801 gctattggat gtaactggag ttttgctcct attgtagata ttaatagaaa ttggagaaat
     7861 cctatcattt ctagtcgatc ttttggacaa aaccctgata aagttttaga acttagttta
     7921 gcttatatga aaggtattca ggagagtgga attttgcctg cagctaaaca tttccctggt
     7981 gacggtattg acgaacgaga tcagcatcta tcttttagta ttaatagttt aggtacggaa
     8041 gaatgggata attcttttgg aaaagtatac tcaggcttaa tagaagctgg attaccttca
     8101 attatggcag gtcatatcca tatgccagct tattcaaaac attttaatcc tgatctgaaa
     8161 gatgaagatt tacttcctgc aactttatca aaagaattaa tcactgactt acttagagga
     8221 aaactcggat ttaatggatt agtagttact gatgctagtc atatgttagg aatgactggg
     8281 gcaatgaaac gatcagaatt attgcccact tctattgcag ctggatgtga tctatttcta
     8341 ttttttaatg atccagatga agactttaat tatatgatgg aaggctatcg taaaggaatt
     8401 atcactgatg atcggttatt agatgcctta aaaagaattt tagggttaaa agctaaattg
     8461 ggattacaca aaaaagcaaa agaacaaatt ttattaccta agcaagaagc tctggaaaaa
     8521 attggtttaa ttgaaaataa atcaattttt aaagaggtgg ctgaccaagc aattacatta
     8581 gttaaagata aacaagatat ttggcctatt gtaccagaaa aatataaaag gattttacta
     8641 gtagatgtac atggggtaaa aggtgggttt ggtgaagtag ttgctggaaa tagacctcaa
     8701 gccattgatt tattgaaaca gattttgtca gcaaaaggat ttgaagttga aaaatacatt
     8761 tcacctgaag aagaattgaa aggattatcg caagaagaaa aaattaaaaa tatagctgga
     8821 atatatgctg ctaagagacc aattaaaaat ttaactaaaa aatatgactt gatcattaat
     8881 atagctaatg ttaatccagg cacagttcaa cgaattgtct ggccagcaag taaaggaact
     8941 ccggatatac cgttttacat tcatgaaatt ccaacaattt ttgtatctgt acagtgtccg
     9001 tttcatttgg ctgatgtacc tcaagtaaag acttatatta atacttatga tggaaaacaa
     9061 gaaactttag aaatattggt ggacaaacta atgggtcttt ctgaatttaa gggagtaagt
     9121 ccaatagatg cttattgtgg tttaaaggat acatttattt aatatttaga taggagtggt
     9181 ttatatgttg tatcccatta tgacagagac gaggcaagta attgacttaa gcggtctctg
     9241 gagatttaaa ttagatacaa atgatgactt acaggaagaa cttgctttag aaccaataaa
     9301 tgaaaaagag agttatccga tttcagttcc ctcttcttat aatgatttat ttgaaacaga
     9361 gaacattaga aatcatgttg gatgggtgtg gtatgaaaaa gaagtgactc ttcctaaaag
     9421 attattagaa gaaagattag ttcttcgttt tggatctgca acacatgaag caaaagtttt
     9481 tttgaatgga aagtttatta ctcaacacaa aggtgggttt acaccatttg agtctgaaat
     9541 taatcggttt gtaaaagacg ggaataatcg aattacagtt gctgtaaaca atattattga
     9601 cgatagtact ttacctgtgg ggattatgaa ggaaacagta caggaaaatg gggatataaa
     9661 aaaggaaaac ttagttaatt ttgatttctt caattatgca ggtattcata gacctgttaa
     9721 gttatataca actccgaaag actatataag tgatattact attaatacaa gaataactga
     9781 tttggatgca agtgtagaat tcaatatcga tacagaatgg accggaaaaa tcttaatagc
     9841 gatatatgac gaagaggctc aacttgtagc tgaagctgaa ggaaagaaag gaatgattca
     9901 gcttaagaat ccaatttttt gggaacctct agacgcttat ttatatagta tgaaaattag
     9961 tttaattgat gataataaag tgatcgatac ctatacagaa gaatttggta ttcgcagtgt
    10021 tcaggttacc aatggcaaat ttttgattaa taataaacct ttttatttta aaggatttgg
    10081 taagcatgag gattcgtatg ttaatggacg tggtttgaat gaggctgtaa atattaaaga
    10141 ttttaattta atgaaatgga taggtgctaa ttcttttaga acatcacatt atccttactc
    10201 agaagaaatt atgcgattag cagatcgaga aggtattgta gttattgatg aagtacctgc
    10261 agtagggtta catttaaatt tcatggccac tttattagat gataatgtgg aaaaacataa
    10321 tacgtggaaa gagatacgaa cagaaaattc tcataaaacg gtgatcaagg aattgataga
    10381 acgcgacaag aaccacccaa gtgttgtaat gtggtctatt gcaaatgaac cagattcgga
    10441 ttccgaaggt gctaaagaat attttgaacc tttagttgaa ttaactaaag aacttgatcc
    10501 gcagaaaaga cctgtaacaa tcgttactta tctaaaatca acgccagatg tttgtaaggt
    10561 aggagatatt gtcgatgttt tatgtttaaa tcgttattat ggttggtatg tttcgggagg
    10621 ggatctggca catgcaaaat atctgttgaa aaaagagcta gatggatgga tggaaagatg
    10681 tcctgatacg ccaataatgt ttacagaata tggagcagat acagtagctg gatttcatga
    10741 tacagtacca acaatgttta cagaagagta tcaagtggct tattacgaag caaatcacga
    10801 agtaatagat acatgtgaaa attttgttgg tgaacaagtg tggaattttg cagattttgc
    10861 aacaagtcaa gggattttaa gagtgcaagg aaataagaaa ggaatcttta ctagagatag
    10921 aaaacctaaa atgattgctt actatctaca gaaacgatgg tcatcaatac ccaatttcag
    10981 ttataaaaaa taatttggat tttttaagaa tgttcaaaaa
//
LOCUS       DADVXU010000091        10851 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.095, whole genome shotgun sequence.
ACCESSION   DADVXU010000091 DADVXU010000000
VERSION     DADVXU010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.095"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            12..84
                     /locus_tag="KB953_002243"
     tRNA            12..84
                     /locus_tag="KB953_002243"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="KB953_002244"
     tRNA            88..160
                     /locus_tag="KB953_002244"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="KB953_002245"
     tRNA            168..249
                     /locus_tag="KB953_002245"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="KB953_002246"
     tRNA            267..339
                     /locus_tag="KB953_002246"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="KB953_002247"
     tRNA            351..422
                     /locus_tag="KB953_002247"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="KB953_002248"
     tRNA            439..524
                     /locus_tag="KB953_002248"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="KB953_002249"
     tRNA            535..608
                     /locus_tag="KB953_002249"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="KB953_002250"
     tRNA            618..691
                     /locus_tag="KB953_002250"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="KB953_002251"
     tRNA            711..783
                     /locus_tag="KB953_002251"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="KB953_002252"
     tRNA            811..884
                     /locus_tag="KB953_002252"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="KB953_002253"
     tRNA            898..971
                     /locus_tag="KB953_002253"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="KB953_002254"
     tRNA            995..1084
                     /locus_tag="KB953_002254"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="KB953_002255"
     tRNA            1097..1170
                     /locus_tag="KB953_002255"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="KB953_002256"
     tRNA            1189..1264
                     /locus_tag="KB953_002256"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="KB953_002257"
     tRNA            1266..1336
                     /locus_tag="KB953_002257"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="KB953_002258"
     tRNA            1350..1423
                     /locus_tag="KB953_002258"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="KB953_002259"
     tRNA            1448..1535
                     /locus_tag="KB953_002259"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="KB953_002260"
     tRNA            1557..1628
                     /locus_tag="KB953_002260"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="KB953_002261"
     tRNA            1651..1740
                     /locus_tag="KB953_002261"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="KB953_002262"
     tRNA            1753..1826
                     /locus_tag="KB953_002262"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="KB953_002263"
     tRNA            1845..1917
                     /locus_tag="KB953_002263"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="KB953_002264"
     tRNA            1921..1993
                     /locus_tag="KB953_002264"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="KB953_002265"
     tRNA            1998..2068
                     /locus_tag="KB953_002265"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="KB953_002266"
     tRNA            2081..2154
                     /locus_tag="KB953_002266"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="KB953_002267"
     tRNA            2166..2237
                     /locus_tag="KB953_002267"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     regulatory      2426..2505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2503..3696
                     /locus_tag="KB953_002268"
     CDS             2503..3696
                     /locus_tag="KB953_002268"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBB6867206.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="KB953_002269"
     CDS             3826..5298
                     /locus_tag="KB953_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB6867207.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="KB953_002270"
     CDS             5381..6169
                     /locus_tag="KB953_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBB6867208.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="KB953_002271"
     CDS             complement(6236..6904)
                     /locus_tag="KB953_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HBB6867209.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="KB953_002272"
     CDS             7019..8731
                     /locus_tag="KB953_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6867210.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10506
                     /locus_tag="KB953_002273"
     CDS             8734..10506
                     /locus_tag="KB953_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6867211.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(10588..>10851)
                     /locus_tag="KB953_002274"
     CDS             complement(10588..>10851)
                     /locus_tag="KB953_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBB6867212.1"
                     /translation="KNGNPISIQAYNQSLRAVSKELNLDKKVSSHMLRHSHISLLTEL
                     GIPLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT         3111 a         1960 c         2501 g         3279 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 agttaaatta aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt
    10561 ttttataagg ggcagaaaag gggcaattta aatttctatt tttcctagtt tttctactag
    10621 ttgattttgc atatttttag ttgtatgagt ataaatcttt aatgttgttt ttgaatcttc
    10681 atgtcctaca cgatccataa tggcttttaa tgggattcct aattctgtaa gtagagaaat
    10741 gtgggaatgc cttaacatgt gagaagaaac ttttttatct aggtttaatt ctttggatac
    10801 tgctcttaaa gattgattat atgcttgaat agatatcggg ttaccatttt t
//
LOCUS       DADVXU010000092        10684 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.096, whole genome shotgun sequence.
ACCESSION   DADVXU010000092 DADVXU010000000
VERSION     DADVXU010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.096"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(150..557)
                     /locus_tag="KB953_002275"
     CDS             complement(150..557)
                     /locus_tag="KB953_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867213.1"
                     /translation="MSFDNTITISIILALVALISPSITAVINNKHVESMKDKEIELQK
                     HDSKTQTIQTTFSTFLNNVGICIGSNTDKNISAVKASGYAVLPYIQNEDIEVMKIFLS
                     RFGYGNTDAEQKSLETYLIDKVLPILNKSLEKL"
     gene            718..942
                     /locus_tag="KB953_002276"
     CDS             718..942
                     /locus_tag="KB953_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867214.1"
                     /translation="MISVKGLGDEIFEAMMSKAQQDIKEKIISAAAQGQTSCTVRSRG
                     TTPSFLLALEDEGILNIKRRDGSVRLFWQF"
     gene            952..1146
                     /locus_tag="KB953_002277"
     CDS             952..1146
                     /locus_tag="KB953_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867215.1"
                     /translation="MKDFDSLGARQQPPNEASPVGVDWQENPLYPGDTCYLTEEGYVP
                     VDAILEYVQQHYPKIELGGI"
     gene            1149..2171
                     /locus_tag="KB953_002278"
     CDS             1149..2171
                     /locus_tag="KB953_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HBB6867216.1"
                     /translation="MANDLTQTTQRSLDEQVISNLGRLQEQGLEMPPGYSPQNALKSA
                     FFELTNNTGGNLLQMAANNQEMKTSISNALLDMVIQGLSPAKKQCYFIKYGNKVQLMR
                     SYFGTMAVLDRVTGGADITPVVVRQGDEFEVAMDGPNMVVKKHETKFENLDNEIIAAY
                     VVIKLTNGKETTTVMTKKQIDQSWAKSKMKDSGPQKEFPEEMAKRTVINRAAKTLINT
                     SNDNDLLVQAAKDTLENEFDNDRKDITPQTEKVATLEQKFFSNKKITEPIQKEPDPIV
                     IPDDIQNEVTRVADVPAHPEIEQAHLIENEDINPIQEELIDIPDFGREEGVEDVSEFE
                     DDEYPF"
     gene            2134..2949
                     /locus_tag="KB953_002279"
     CDS             2134..2949
                     /locus_tag="KB953_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HBB6867217.1"
                     /translation="MSQNLKTTSTLSDENYYSNEADWQYMSTSQYKSFLKCEAAALAK
                     LKGDWAPTSDPKALLVGNYVHSYFESKEVHEAFKEENKSKMFSSRKPYGLLKDFQIAE
                     QMIERLKQEEAFMNIYQGEKETIVTGELFGTTWKGKIDCLNVEDEYFVDIKTTKDMHE
                     RKWNENYGSRETFIVNFGYVLQMAIYQELLLQQYGKMFMPIIAAVSKQTPSEAKLITI
                     DQDKMSYELIMLKENIERIVKVKNGEERPIPCGTCEYCRGNLSITGFTSMDDL"
     gene            2967..3749
                     /locus_tag="KB953_002280"
     CDS             2967..3749
                     /locus_tag="KB953_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867218.1"
                     /translation="MNLGYIKLYRKVTSSFVWTNSDMFKLWILCLMKASHEDRKFLFN
                     GQEVRLTSGQFVTGAHAIAKEYNEGVPSDKAIAWRTLWRWLKKFENEELLTIQSNARY
                     SVITIINWSDYQSSDKPVTSDGQSSDKPVTTNKNDKNDKNDKNNNPRNSRKTREYADD
                     DPNKKLAILLLKLIRKNRSLKEPDLNKWANTIRLTIEADKRTGKEVQDMIVWATNHDF
                     WSTVISSPTSLRKNFDTMVGQKNKRKQQNISNDELPETGEDW"
     gene            3749..4597
                     /locus_tag="KB953_002281"
     CDS             3749..4597
                     /locus_tag="KB953_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBB6867219.1"
                     /translation="MNKKLSAMAAPYGGLKTADHNCPKCGDPLYIWKTKNKDGTDRCG
                     PTCINKICGYREMVTKNQKEAIQKANEAMKKDAINRMLNSSMITDDAIWTFDFDGYKV
                     VDQETAQIKAMAQEWAKKIVNGSTIHAVITGRTGAGKTHLGAAVIKEVMRASNYKIAC
                     SFISYRELLEQLKFAMNDPEARKAVTGSLMAEIKKSDFVVIDDLGAELGRMEENNQAT
                     PYDVDVLTSLTEARLNKATIFTTNLSSKQLKHAYGERVFSRVMNGTKGNIAVFKTTTD
                     KRRNPV"
     gene            4594..4836
                     /locus_tag="KB953_002282"
     CDS             4594..4836
                     /locus_tag="KB953_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867220.1"
                     /translation="MTFVVKKMCYLDSRGRGEASVEFAKHHTTKEEADLVASVCGGEV
                     VEVIKPKRRFSKPKTKPVKKECCCPKSNQAWMRGAK"
     gene            4979..5347
                     /locus_tag="KB953_002283"
     CDS             4979..5347
                     /locus_tag="KB953_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HBB6867221.1"
                     /translation="MATRSKYGNKKHEVDGITFDSITEAEYYDLALWQAQANGWKVKL
                     QERFELMPKFELEGKKYRKIEYIPDFTFYKSGKLVKVVDVKGMQTKDFKIKAKLFCHQ
                     YQVPLILAKKYRNTFKEERF"
     gene            5362..5523
                     /locus_tag="KB953_002284"
     CDS             5362..5523
                     /locus_tag="KB953_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBB6867222.1"
                     /translation="MTTEEVIQMRIRSLRREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            5520..5846
                     /locus_tag="KB953_002285"
     CDS             5520..5846
                     /locus_tag="KB953_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867223.1"
                     /translation="MSVDKEAAWRKLMLLIQDENWQEDEAVVAEVQRLGKIANGRIRK
                     KPDKRKQRKGKIVVVLHEGKILMQGTASELSAETGYTRGTIRTYAWRNHTDRKGHEYK
                     YLEEEK"
     gene            5843..6658
                     /locus_tag="KB953_002286"
     CDS             5843..6658
                     /locus_tag="KB953_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HBB6867224.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            6642..6785
                     /locus_tag="KB953_002287"
     CDS             6642..6785
                     /locus_tag="KB953_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867225.1"
                     /translation="MKQANNMGFFMAKILLFLGLVGAAYLVYALLSQTDDKEDDNNDE
                     MKF"
     gene            6797..7108
                     /locus_tag="KB953_002288"
     CDS             6797..7108
                     /locus_tag="KB953_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBB6867226.1"
                     /translation="MDELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEGS
                     K"
     gene            7132..7350
                     /locus_tag="KB953_002289"
     CDS             7132..7350
                     /locus_tag="KB953_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867227.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            7347..7763
                     /locus_tag="KB953_002290"
     CDS             7347..7763
                     /locus_tag="KB953_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HBB6867228.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIYENSELLEVE"
     gene            7760..8098
                     /locus_tag="KB953_002291"
     CDS             7760..8098
                     /locus_tag="KB953_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867229.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDMYGNSESAIKLFGAIEIYADDF
                     PNLWLKEEAE"
     gene            8095..8325
                     /locus_tag="KB953_002292"
     CDS             8095..8325
                     /locus_tag="KB953_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867230.1"
                     /translation="MKYEIPLSEADVQSIINGREVNKKLPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSINDTFRLGS"
     gene            8332..8535
                     /locus_tag="KB953_002293"
     CDS             8332..8535
                     /locus_tag="KB953_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HBB6867231.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASDAIRKFGISILSDDRKWEEKE"
     gene            8532..8828
                     /locus_tag="KB953_002294"
     CDS             8532..8828
                     /locus_tag="KB953_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867232.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EEFIYPFRGYVEHVYNHSAIIRIENTMECDKWTAKSKENLAVVRLVDMELINDK"
     gene            8905..9318
                     /locus_tag="KB953_002295"
     CDS             8905..9318
                     /locus_tag="KB953_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HBB6867233.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDALIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            9331..9717
                     /locus_tag="KB953_002296"
     CDS             9331..9717
                     /locus_tag="KB953_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867234.1"
                     /translation="MWKDYVSLKELKKDLVFKRIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHTSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKDKLCIVTDA"
     gene            10268..10495
                     /locus_tag="KB953_002297"
     CDS             10268..10495
                     /locus_tag="KB953_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867235.1"
                     /translation="MRTYWYVSLNNKYPKPMKGQHRRVVMSVQMKAKYSIVEMLREAT
                     PKEIDQYKLVLIGIGWFKDEHIQTNMKRWLR"
     gene            10492..>10684
                     /locus_tag="KB953_002298"
     CDS             10492..>10684
                     /locus_tag="KB953_002298"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867236.1"
                     /translation="MKLYYVEKANCWVVEGCLSKYATDIISTQFSELGLKVIFCGEFV
                     SVKEAVLPMTAKSRRSRGSS"
BASE COUNT         3855 a         1651 c         2424 g         2754 t
ORIGIN      
        1 tacagagcga aaaagcccac cgagggggct gactaaagtg tggttctgtt tattaggtgt
       61 ttatctcgtg gccgttttag taaataacta catgagaaaa cgtggcgaat actggtatac
      121 atcctatgcg gttctagtat gtctaatgct tacaattttt ctaatgattt attcaaaata
      181 ggtaatactt tgtcaatcaa ataagtttca agagattttt gttctgcatc agtgttgccg
      241 taaccaaaac ggcttaagaa aattttcata acctctatat cttcattttg aatatatggc
      301 aggactgcat aacctgatgc ttttacagca gatatatttt tatcagtgtt actaccaata
      361 caaataccca cattgttgag aaatgttgag aaagttgttt gtattgtttg ggtttttgaa
      421 tcgtgttttt gtaattcaat ttctttatcc ttcatcgatt caacatgctt attatttata
      481 accgctgtaa tcgaagggga tataagagct actaaagcta ggataatcga aattgtgatc
      541 gtgttatcga aactcatttt ttcaccatcc agtttttaaa ccattatatc aaaaaggaga
      601 gaagaaataa tgcaagaatt agtaattttg aaaaataaaa aaagacacga acgccagcaa
      661 gcatgatcgt gtccaaaaga aaaatctttt aaaccattat aacacaggag gaaataaatg
      721 atctcagtta aagggttagg cgatgaaata ttcgaagcaa tgatgagcaa agcacaacag
      781 gacatcaaag aaaaaatcat ttctgctgca gcgcaaggac aaactagctg cactgttcga
      841 tcgagaggaa cgacaccaag ttttcttttg gcgctagaag atgaaggcat tttgaatatc
      901 aaacgaagag atggcagcgt cagattgttt tggcaatttt aggaggccgt gatgaaagat
      961 tttgattctt taggcgccag acagcaacca ccaaatgaag ctagtcctgt tggagttgat
     1021 tggcaagaaa atccgttata tccaggtgat acttgctatc taacagaaga aggctatgta
     1081 ccagtagacg caattctaga gtacgtccag caacattatc caaagattga attaggagga
     1141 atttaggaat ggcaaatgat ttaacacaaa caacacaacg ttcattagat gagcaggtaa
     1201 tcagtaatct tggacggtta caagaacaag gtttagaaat gccaccagga tatagtccac
     1261 aaaatgcgtt aaaaagtgct tttttcgaac tcactaacaa tacaggagga aacttgttgc
     1321 agatggctgc aaacaatcaa gaaatgaaaa cctccatttc taatgcactt ttagacatgg
     1381 ttattcaagg gttatctcca gcaaaaaaac aatgctattt cattaaatac ggaaacaagg
     1441 ttcagctaat gcgttcatat tttggaacaa tggcggtcct tgatcgtgta actggagggg
     1501 cagatattac gcctgtagtc gttcgacaag gtgatgagtt tgaagttgca atggatggac
     1561 caaacatggt tgtcaaaaag catgaaacaa aattcgagaa tttagacaat gaaatcattg
     1621 cagcctatgt agtcatcaag ttaacaaatg gtaaagagac cacaacagtc atgacgaaaa
     1681 agcaaatcga tcaaagttgg gctaagtcaa aaatgaaaga ttctggaccg caaaaggaat
     1741 ttccagaaga aatggctaaa cggacagtaa tcaatcgggc agctaaaaca ttgatcaaca
     1801 caagcaacga caacgattta ttagttcaag ctgcaaaaga tacgttagaa aatgaatttg
     1861 acaatgatcg gaaagatata acgccacaaa cagaaaaagt ggctactctc gaacaaaaat
     1921 tcttttcaaa caaaaagatt actgaaccaa tacaaaaaga accagatccg attgtgattc
     1981 cagatgatat ccaaaatgaa gtaactcgag ttgctgatgt acctgcccat ccagaaatag
     2041 agcaagcaca tttgatcgaa aatgaggata tcaatccaat tcaagaagaa ttgatagata
     2101 ttccagactt tggacgcgag gaaggtgtag aagatgtctc agaatttgaa gacgacgagt
     2161 accctttctg atgagaatta ttactccaat gaagctgact ggcagtacat gtcaacatca
     2221 caatataagt cctttttaaa atgtgaagcc gcagccttag caaagttgaa aggcgattgg
     2281 gcaccgacgt cggatcccaa agccttactc gtaggaaact acgtacattc ttattttgaa
     2341 tcaaaagaag tacatgaagc gttcaaagaa gaaaataaat ccaaaatgtt ttctagtagg
     2401 aagccgtatg gattgttgaa agatttccaa attgctgaac aaatgattga acgacttaag
     2461 caagaagaag cattcatgaa catttatcaa ggtgagaaag aaacgatcgt gactggggaa
     2521 ctatttggaa cgacatggaa aggcaagatt gattgcttga atgttgagga tgagtatttt
     2581 gttgatatca aaacaaccaa agacatgcat gagcggaaat ggaacgaaaa ctacggatca
     2641 agagaaacgt tcattgtcaa ttttggttac gtacttcaaa tggcaatcta tcaagagctt
     2701 cttttacagc aatacggaaa gatgttcatg ccaatcatcg ctgcggtatc gaagcaaacg
     2761 cctagtgagg cgaagttgat aacgattgat caagataaga tgagctacga gctaatcatg
     2821 ctaaaagaaa atattgaaag aattgtcaaa gtgaaaaatg gcgaagaaag gccgatacct
     2881 tgcggtacgt gcgagtattg tcgaggtaac ctttcaatta ctggattcac tagcatggac
     2941 gatctttaaa aggaggtgct ttgcgaatga atctaggata catcaagtta taccgtaaag
     3001 tgactagttc attcgtttgg accaattccg atatgttcaa gctatggata ttgtgtttaa
     3061 tgaaggctag tcatgaagat agaaaatttt tatttaacgg tcaagaagta cgtttgacaa
     3121 gcggacaatt cgtcacagga gcccatgcaa tagcaaaaga gtacaacgaa ggagtcccga
     3181 gtgacaaagc aattgcatgg cgaacgctat ggagatggct taagaaattt gaaaatgaag
     3241 aattattgac aatccaatcg aacgctagat atagcgttat aacaataata aattggtctg
     3301 actatcaatc gagtgacaag ccagtgacaa gcgacggaca atcgagtgac aagccagtga
     3361 caacaaacaa gaatgataag aatgataaga atgataagaa taataatcct cgcaactctc
     3421 gaaaaacacg agagtatgca gatgacgatc caaataaaaa attggccatt cttttattaa
     3481 aactcattcg aaagaatcgt agtctcaaag aacccgattt gaacaaatgg gccaatacga
     3541 ttcgtttgac gattgaagct gacaaacgaa ctggaaaaga agttcaagac atgattgttt
     3601 gggctactaa ccatgatttt tggtccacag tcatttcatc gccaactagc ttaagaaaaa
     3661 atttcgatac aatggtgggt caaaaaaata aaagaaagca acaaaatatt tctaatgatg
     3721 agttaccaga aacaggtgag gattggtaat gaataaaaaa ctaagtgcaa tggctgcacc
     3781 ttacggcgga ttaaaaacag cagatcataa ttgcccgaaa tgtggtgatc cattgtatat
     3841 ctggaaaaca aaaaataaag atggtactga tcgatgcggt cctacatgta tcaataagat
     3901 ttgtggttat cgagaaatgg taactaaaaa tcaaaaagaa gctatccaaa aagcgaatga
     3961 agcaatgaaa aaggacgcta tcaatcgaat gcttaacagt tcaatgatta cagatgatgc
     4021 catatggacc ttcgattttg atggatacaa agtagttgat caggaaacag cacaaataaa
     4081 agcgatggct caagaatggg ctaaaaaaat tgtaaatggt agcacgattc atgcagtgat
     4141 aaccggtaga acaggagccg gcaaaaccca tctaggcgct gcagtgatta aagaagtcat
     4201 gagggcatct aattataaaa ttgcttgttc atttatcagt tatcgagaac ttttagaaca
     4261 gttgaaattt gcaatgaatg atccagaagc aagaaaagcc gtaacaggtt cgctgatggc
     4321 tgagataaag aaatcagatt tcgtggtaat cgacgatttg ggtgcagaac taggaagaat
     4381 ggaagaaaac aaccaggcaa cgccttatga tgttgatgtt ctcacatcgc tcacagaagc
     4441 tcgtctaaac aaagctacga tattcacaac caatttatca tcgaagcaat taaaacacgc
     4501 gtacggtgag cgagtgttct ctcgtgtaat gaatggaacg aaagggaaca tagctgtatt
     4561 caaaacaacg acagacaaaa ggaggaatcc agtttgacct ttgtagtgaa aaaaatgtgc
     4621 tacttagata gccgagggcg aggagaagct agtgtcgagt ttgctaaaca ccacacgact
     4681 aaagaagagg ctgaccttgt tgcaagtgtt tgtggcggcg aagtagtcga agttattaaa
     4741 cccaaaagac gattttcaaa acctaagaca aagccagtga aaaaagagtg ttgttgtccg
     4801 aaaagtaacc aagcatggat gagaggtgca aaatgacttg tttaaaatgc aacgatgaaa
     4861 cagttatttg gtataagaca ttgcttggat ggtcaacttg tgaaccttgt ccaatatgca
     4921 atgaaaatgg acgacgttcg aaagaacgac tcgaaagact aaagaaggag tatagcaaat
     4981 ggcaacaaga agcaaatacg gaaacaaaaa gcacgaagta gacggcatca cttttgattc
     5041 tatcacggaa gcagagtatt acgatctagc cttgtggcaa gcacaggcga atggttggaa
     5101 agtgaagctc caggaaaggt ttgagctgat gccgaagttt gaactagagg gcaagaagta
     5161 tcgcaagatc gagtatattc ccgacttcac attttacaaa agcggcaagc tggtcaaagt
     5221 cgtagatgtt aaggggatgc agacaaaaga ctttaagatc aaggcaaagt tgttctgtca
     5281 tcaatatcaa gtgccgttga tattagccaa aaaatatcgg aatacgttca aggaagagcg
     5341 tttttaacga ggtggtccat catgacaaca gaagaagtga ttcaaatgcg aattcgaagc
     5401 cttcggcgtg agattgacga tctggaacga acaaaggcag tgatggtcaa tgaaacggct
     5461 agaaaggcaa tcgatttgca catagagaat ttaagaaggg aaatccgtag attggaggaa
     5521 tgagcgtgga taaggaagcg gcttggcgaa aattaatgtt gctgattcaa gatgagaact
     5581 ggcaagaaga tgaagcagtg gttgctgaag ttcagcgtct aggaaagatt gctaacggac
     5641 gtatacgaaa aaagccagac aaaagaaaac agcgcaaagg gaaaatcgtc gttgttttac
     5701 acgaaggcaa aattttgatg caaggaacag ctagtgagct gtctgcagaa actggatata
     5761 cgcgtgggac tattcgaacg tacgcttggc gaaatcacac cgatcgaaaa gggcacgaat
     5821 ataagtattt ggaggaagaa aaatgaacga aaacaaatta atcaaattgg gtgtagcagg
     5881 agcagtaatc gtaggtattg gagttatcgg aggatttaag ttcttcgaaa aaatcgataa
     5941 tggatatgtg ggtgtgcgct attcaatgaa cggcggtatc aaagatgaag cactgacgca
     6001 aggtgtgaaa tttgtaggga ttgacaaagt gatccaatat ccaattcgct tgcaaactat
     6061 ccaatcaaaa aatatttcag tatctacaag cgacggcaaa aagacaacga ttgatatcaa
     6121 atatgactac aaagttgatt caactaaagc agcaaaaatg tacaaggaat ttgggaatat
     6181 cacttcggaa gatatcgaaa gtggatggtt aaaatctaag cttcaaaagg tcgctcgtga
     6241 agtttatgcg aaatatagtc tgcttgatgt cctttcagga gattcctcta aagttgaagc
     6301 tgaggtatta acgaactttg ctaaatcagt tgaatctaaa gggtttgaag tcgaagacgt
     6361 aacacttggt gttccagatg tcgataaaga aacacaaaaa tcaatcgatg cgatcattcg
     6421 agctggtcaa gaaaatgaaa aagcgaagct agacgcagaa actgcaaaaa ctcaagctga
     6481 tagtgaagct tacaagaaaa caaaagctgc agaagcagag gcagaatcta atcgcaaagt
     6541 cgccgaatca gtaacagaca atttgattcg ttatgaagaa gctcaagctc gcaaaaagca
     6601 tggatgggta acagtaaatg gagcagatac tgtagttacg gatgaagcag gcaaataata
     6661 tgggattctt tatggctaaa attctcttgt tcttaggttt agtaggagca gcatatctcg
     6721 tgtatgcgct cctttcccaa actgatgaca aagaagatga caacaacgat gaaatgaaat
     6781 tttaggagga gaaataatgg acgaactaat cacaaaagta gagcagtggg ctaaagacaa
     6841 gggactggat caagcagatc caaaagcaca gtttttgaaa gtagctgagg aattcgggga
     6901 aatagcttcg gcgatggcaa gaagtaatga tgaactattt aaagatagcg taggagacgt
     6961 tatcgtcacg ctgattatcc tttccatgca aaaagggaca aacgtacaag agtgtttaga
     7021 aatggcatac aacgaaatca aaggacgcac agggaaaatg gtagatggtg tattcgtgaa
     7081 gtcgagtgat ttggagggca gcaaatgata aaaaagctca ttcaattcag catggattta
     7141 tatgatatcg aatcaggagc aacactatct gtggaatcgg accatctaat cataaatttt
     7201 ggtggaaagc gccagattat tttgtgggta gttgatgatg tactgtttcc agaaattgtt
     7261 catgatttcg aagaatcaaa agcggttgag tttgaaatag tgaaaaaagt aatggaattg
     7321 attgaaaaat acgaggagga cagcgaatga taccgaagtt tagagcgtgg gataaacgaa
     7381 agaacgtaat gagagatgta gccgtcttgc attttactaa aaacggcaaa acaaacttta
     7441 ttgaatattg gataaatcct accgaattga aatcatatca cgtgcgaaac atcgacctca
     7501 tgcaatccac aggaatgaaa gataagaatg gtgtggatat atttgaaggg gatatagtat
     7561 tagtcagcgt gcgaaatggc ttcgattact tagataataa agtttgtatt gtcaaaaatt
     7621 caatagatta ttccggatta gtttgtgcca ctgttgatga agacttagag tatcaaattt
     7681 ttaacacaga gctgtttgaa gaatacacgt atgaagtcat cggaaatata tacgagaata
     7741 gcgagttatt ggaggtagag tgattatctt agctactgac tacatcgatt ctttaaaaga
     7801 tgaaaatggg aatgtcccag cattgctaaa ttgtggaatt aaaagcaaga atagaatcat
     7861 tttgttacgg atatcggctg atttagcaaa atatctttat ctgagcaaga cagcagtata
     7921 ttacactgcg gctacacgtt atcagtacaa gggaaaaaca atcagtcagg attattatga
     7981 tcgtttaatc agtcctgata tgtatgggaa ttcagaatca gcaattaagc tgtttggcgc
     8041 aatagagata tatgccgatg attttccaaa tttatggttg aaggaggaag cggaatgaaa
     8101 tacgaaatac cactaagtga agcagacgtc cagtcaatta ttaacggtcg ggaggttaat
     8161 aaaaaacttc ctgatggtac tgaattagtc atcagacaaa gttatttgaa agatatggca
     8221 gctccagtat taattgatcg ttttaacgtg actgattctg tggtagagaa ccacttgaaa
     8281 gaatttcgat caagtataaa cgacactttc agattaggga gttgattgac aatgaacacc
     8341 agacaccgca gagtagcaaa actaagaaaa caggaactga atgtactaaa gacaaagttt
     8401 gaaaaagaat atggaatttc agcagaagaa acatataaag tggcaagtca gtgtgttgct
     8461 gatgcaagtg atgctattcg taagtttgga atttcgatat taagtgatga tcgtaaatgg
     8521 gaggaaaaag aatgaaacta aaagacggat tttacgctag cagtcacggt atcggcggtt
     8581 taatgctaga tatgccgaca aagaatccta aaacacgtaa gaaaccaaaa gtcaaagtcg
     8641 gtgacatggt ccgctgtgaa gcagaggagt tcatttatcc atttcgtgga tatgtagaac
     8701 acgtctataa tcactcagcg atcattcgca ttgaaaacac gatggaatgt gataaatgga
     8761 cagctaaaag caaagagaat ttagcagtag tgagattggt ggatatggaa ctaatcaatg
     8821 acaaataaaa aagccggatc gctccgacta acataataaa acagacaagt ttattatatc
     8881 acataaaagg agcagtttga attgatgcaa ttgttacgag aggtagattt caaacagaca
     8941 agatgtaatg cgagagatgt gctgaagaac tttcggcgtt tggagcggat ggcaggtcgc
     9001 tctttgatag atattaagtc gccgattatt acggatatgc cgaaggcacc gaagcacggc
     9061 aataaggcag aggatgcatt gattcagatg atggatatag aagcggagag agacgcgatt
     9121 ttagcggctt tgatggctct tagtctgatt agtcgtcaga tactctacta cagtttttgt
     9181 gtgccagaca gcttctcaaa ctacagaatt agccgtgaag tgggttattc agaaagaagt
     9241 atacaacgga tgaaatcgga agctctaata gagtttgcag aagcatataa acacggaaga
     9301 ataattgctt ataaataggg ggaccatata atgtggaaag attatgtatc gttgaaagag
     9361 ttgaaaaaag atcttgtttt caaaagaatt gtagaatggt cagagagtga attaatttta
     9421 gaagatggaa ccaaaatgga agttgtatgt agcgaatcag attgctgcgc atgggccgaa
     9481 ggtgaattca aaaatgtaaa gcttgatgca gtgattacag atataaagat ttttgataaa
     9541 ggtaaccgtc tctataatgg tgacggacat acctcttatg ctgaagtcgt tgtttatcat
     9601 aatcgtaatg agatcgctaa agcagaatgc acagcgaatg atgggaatgg cggctactat
     9661 tatagtgttt gtgctttaaa ggtcaaagac aaactttgta tagtgactga cgcataaaaa
     9721 aatggcggtt ttttggcgga atgatggcgg tttttagcca tttatcagtg atattatggt
     9781 agtgtcgaaa gattagtgat aggtctgaga caaaataata ataaaaggaa catcgtttta
     9841 ttattgtttc acaattaagc ttcgatagac agcaacggaa atattaagaa taaggatgtg
     9901 aattttaact ccttctaaat tgttcttatt atctatcatc cgttgctgtt tattgttaca
     9961 tacagatcgc ttaggcggtc tttttatttt ggccgaaaac ctacattttc gatggccgat
    10021 tattggagga ggaatagcat gttcaaacta tctgaaatca ttaagaagtc cgacgttgag
    10081 aaattagaga tgttaaaaaa gaagttgaag aaatagcagt ctcttcgtga ggctgtttta
    10141 ttttgctcac aaaaatagac cactatcggg taatagtggt caggaattaa atgaaaaaga
    10201 tgttagggtt gttagctaag tataacatca taacgcttac aaaacaatgc aagaaggata
    10261 aattattatg agaacttact ggtatgtgtc attaaacaat aaatatccga agccaatgaa
    10321 aggacagcat aggcgtgtag tcatgtctgt tcaaatgaag gcgaagtatt cgattgtaga
    10381 aatgctaaga gaagctacac caaaagagat tgatcagtat aagttggttc ttattggcat
    10441 tggttggttt aaggatgaac atattcaaac aaacatgaag aggtggttga gatgaagtta
    10501 tattatgttg aaaaagcaaa ttgttgggtg gttgaaggct gtctgtcaaa gtacgctaca
    10561 gatattatat ctactcaatt ttcagagttg ggattgaagg ttatcttttg tggtgagttt
    10621 gtcagtgtga aagaagcggt attgccaatg acagcaaaga gcagacgctc gcgtggttca
    10681 agtt
//
LOCUS       DADVXU010000093        10650 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.097, whole genome shotgun sequence.
ACCESSION   DADVXU010000093 DADVXU010000000
VERSION     DADVXU010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.097"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            23..646
                     /locus_tag="KB953_002299"
     CDS             23..646
                     /locus_tag="KB953_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867237.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /locus_tag="KB953_002300"
     CDS             1096..2145
                     /locus_tag="KB953_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBB6867238.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="KB953_002301"
     CDS             2142..3023
                     /locus_tag="KB953_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6867239.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="KB953_002302"
     CDS             3026..3796
                     /locus_tag="KB953_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBB6867240.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="KB953_002303"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="KB953_002303"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBB6867241.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="KB953_002304"
     CDS             5107..6270
                     /locus_tag="KB953_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBB6867242.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="KB953_002305"
     CDS             6387..6797
                     /locus_tag="KB953_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBB6867243.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9199
                     /gene="topB"
                     /locus_tag="KB953_002306"
     CDS             6971..9199
                     /gene="topB"
                     /locus_tag="KB953_002306"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBB6867244.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9370..9654
                     /locus_tag="KB953_002307"
     CDS             9370..9654
                     /locus_tag="KB953_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867245.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9814..10050
                     /locus_tag="KB953_002308"
     CDS             9814..10050
                     /locus_tag="KB953_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBB6867246.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10047..10445
                     /locus_tag="KB953_002309"
     CDS             10047..10445
                     /locus_tag="KB953_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HBB6867247.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3717 a         1773 c         2253 g         2907 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg
     9121 gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa
     9181 tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg
     9241 agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta
     9301 ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta
     9361 aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct
     9421 gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg
     9481 attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca
     9541 agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc
     9601 attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac
     9661 gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa
     9721 aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact
     9781 atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt
     9841 agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt
     9901 gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc
     9961 aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa
    10021 gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa
    10081 tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa
    10141 atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa gaactctatc
    10201 ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc
    10261 ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt
    10321 gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt
    10381 cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa
    10441 aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa ggaatatgct
    10501 gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga aaatatggat
    10561 taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg aaatagtggt
    10621 ttagaactag tgggctgatt ttttatatat
//
LOCUS       DADVXU010000094        10372 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.098, whole genome shotgun sequence.
ACCESSION   DADVXU010000094 DADVXU010000000
VERSION     DADVXU010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.098"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            6..305
                     /locus_tag="KB953_002310"
     CDS             6..305
                     /locus_tag="KB953_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBB6867248.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="KB953_002311"
     CDS             378..1739
                     /locus_tag="KB953_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBB6867249.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="KB953_002312"
     CDS             1761..2021
                     /locus_tag="KB953_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867250.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="KB953_002313"
     CDS             2035..2787
                     /locus_tag="KB953_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBB6867251.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="KB953_002314"
     CDS             complement(2881..3486)
                     /locus_tag="KB953_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBB6867252.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="KB953_002315"
     CDS             complement(3539..4513)
                     /locus_tag="KB953_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HBB6867253.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="KB953_002316"
     CDS             complement(4891..5610)
                     /locus_tag="KB953_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBB6867254.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="KB953_002317"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="KB953_002317"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBB6867255.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVYEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="KB953_002318"
     CDS             7161..8558
                     /locus_tag="KB953_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBB6867256.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="KB953_002319"
     CDS             8639..9079
                     /locus_tag="KB953_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBB6867257.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="KB953_002320"
     CDS             9227..9553
                     /locus_tag="KB953_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBB6867258.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="KB953_002321"
     CDS             9554..10303
                     /locus_tag="KB953_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867259.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
BASE COUNT         3289 a         1830 c         2150 g         3103 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atatacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tc
//
LOCUS       DADVXU010000095        10083 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.099, whole genome shotgun sequence.
ACCESSION   DADVXU010000095 DADVXU010000000
VERSION     DADVXU010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.099"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(109..2229)
                     /locus_tag="KB953_002322"
     CDS             complement(109..2229)
                     /locus_tag="KB953_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBB6867260.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2459..3637
                     /locus_tag="KB953_002323"
     CDS             2459..3637
                     /locus_tag="KB953_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HBB6867261.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3653..4411
                     /gene="aroD"
                     /locus_tag="KB953_002324"
     CDS             3653..4411
                     /gene="aroD"
                     /locus_tag="KB953_002324"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBB6867262.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4434..4919
                     /gene="ybaK"
                     /locus_tag="KB953_002325"
     CDS             4434..4919
                     /gene="ybaK"
                     /locus_tag="KB953_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HBB6867263.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4990..6243)
                     /locus_tag="KB953_002326"
     CDS             complement(4990..6243)
                     /locus_tag="KB953_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBB6867264.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6360..7709
                     /gene="gdhA"
                     /locus_tag="KB953_002327"
     CDS             6360..7709
                     /gene="gdhA"
                     /locus_tag="KB953_002327"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HBB6867265.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFISGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7821..9167
                     /locus_tag="KB953_002328"
     CDS             7821..9167
                     /locus_tag="KB953_002328"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBB6867266.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9475..9861)
                     /locus_tag="KB953_002329"
     CDS             complement(9475..9861)
                     /locus_tag="KB953_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBB6867267.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(9910..>10083)
                     /locus_tag="KB953_002330"
     CDS             complement(9910..>10083)
                     /locus_tag="KB953_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HBB6867268.1"
                     /translation="TVETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQ
                     RRFEGTLRYSLGY"
BASE COUNT         3215 a         1839 c         2053 g         2976 t
ORIGIN      
        1 ctgagccaga atgaggttca ttctgaccca gatttgattt tgtacaaccc atcagtcctt
       61 attgacaaag agcctaattt tgtcgctgct ccttttaaat ttattttatc attcggaaga
      121 acttgtggtc gttccgtgaa gttcttggta cgtttgcgtt ttataaagat caacagttga
      181 ttgtccgttg tgttcactaa agacactagt tgacttgtca ccaagagatt tttcagcttc
      241 ttttaatttc tctaagccat tcttgtagtc ataatcactg gtatcaattg ggtctaatcc
      301 actattcgta tagaaccgaa gaagatcacc attattgatt tggtcagaaa cagctaactg
      361 tttatcaact ttttctttca gtgaatcaat ttcattctgt acttcctcag taggttcagt
      421 aatagcttca ccagtttcgt tactatagat cgtaccaccg tagtaagtat attttggtgt
      481 aacaaaatca ccatcacgga aagaaacaag ctgttcatga tctttcgaga atagatcttg
      541 tcctaactga atataatttt ttgtatctac acctagtaag tgaagtaatg taggcaaggc
      601 atcaacttgt ccgccgtaag tgtgattgat tccgccctgt gtttgtccag ggatatggat
      661 catataaggg acacgctgca tttgtgcatt gtcgtagttt gtccaagtag aacttgtttt
      721 tccaactaat tcagctagat ttttatttct tgaattcgag acaccgtaat ggtcaccgta
      781 aaggacgatg actgaattat catacaagcc gctttctttc aaataattga agaattcttg
      841 tacggcggta tctaaataat tggctgtcgc aaagtaaccg ttgatcgttt catcatttgt
      901 attggctaac gggaatcctg cttcgtcatt ggtgaatttt gaataaggat aatggttaga
      961 tacagcaatg aattttgaat aaaatggttg ttgcagacgt tccaagtatt gtacggattg
     1021 ttggaaaaat ggtttatcat gcaacccgta ttggaacgaa ttgtcatcat taacatcata
     1081 ataagaagaa tcaaagaaat aatcatatcc taaattcttg tatgtctcgt ttcgattcca
     1141 gaatgtacca gcgttcccat ggaataccgc actagtgtaa ccttgtgttt gacttaaaat
     1201 atcaggtgca gcttggaaag tattttttcc gcctaattgt gtgaacaagg agccttgatt
     1261 caagccaaac aatgaatttt ccatcagcgt ttccgcatcg cttgtttttc ctgcttttac
     1321 ttgatggaaa aagttgtcaa aactgaacgt gctgttgctg tggtaaagac tattgatgaa
     1381 aggtgtaact tcatgctgga gaccattctc atctgtcaat ttataatcga tcaggaattg
     1441 ctggaagctt tctaaatgga tataaatcac attttttcct ctagcgagcc cgaacaaatc
     1501 gtcatttggt gctgcataat gttcttttac ataattttct acttctttca tatcattggg
     1561 acttgcttct gcccgcactt ggtttgtctg gtaagtttga acaccgtcat aaaccatgaa
     1621 agcattcaag ccaagatatt tgaccaaata atcacgagag aatgttcgag tcagcaattc
     1681 tggacgatca gcttccgcca taaaaagatt tcctgagaaa atcataactg ctaatgtaga
     1741 gatcgcaaaa gccattcttg ctcggatcgg gcgagggtcc aacttgattt tttttgcaat
     1801 cagcagaaca gtcaagataa taaaatcgat ccaatagatc acatcatatg ggcgaaacag
     1861 tcgaatggca ctttctccca gaccactcgc tactttccca gcacctaaaa tcgtgttgat
     1921 cgtaatgaag tccgtgaatt ctcgataata agtgacattc gaaaaaacaa gcaatgtcaa
     1981 aagaaaataa atgaccatca tcgtgatata agcaactcta gggcgtctga tataaagagc
     2041 aattccaagt aataacaatg tagttgcaat agggttgata aacaagataa agtattgaat
     2101 gccactttcg attccgagat ggaaatcaac taaataggca aaaatcgttt tcaaccaaag
     2161 taaaacgact agtaaagaaa aaaatccgag cctcttattc aaaaaggcag gtgtttttat
     2221 acatttcaag ataatcttcc tttctgcact tcttaatcac agaagtgtcc tgaaaccatg
     2281 ttacacagct cacttattct catttatttt actagtatga caatatagtg tcaattgaaa
     2341 ccagaagttt atgctgtttc attaaggttt tctttaagct tcatgaaaaa atgagcagta
     2401 agacaagtcc tatgtttttt gttatacttg ggaagagaaa gctaaagcag gtgaaggaat
     2461 gaaagtaaaa gtaaaaaaaa gagcaagcaa tcgattgaaa aaaggatatc cactgatcca
     2521 aaaagaagac cttgctcatc cccaaaaaga aaccacgcaa tgggtggaat tagttgattc
     2581 gcaaaaccaa tttatcgcaa aaggatatct aggaaagcag aataagggaa tcggttgggt
     2641 attgagccag cagaacatcc catttgatca atcttttttt gaagaatttt ttgtgaagtc
     2701 aaaagaaaaa agaactttct ttttttcaga tgattctact accgcttttc gtatattcaa
     2761 tggagaagga gacgggatcg gcggtctgat tgttgattat tatgcaggtt atgctgtttt
     2821 ttcttggtat aacgaaacgt tatatgcaca tcgtgaatta ttgattcaag cttttcgtga
     2881 aatctacccg gaaatcatag gaggctatga gaaaattcga tttgaatcag taaatctgcc
     2941 tgagagccag ttcatttacg gaaaagaggc accggaaccc ttgatcgtaa aagaaaacgg
     3001 agtaaaattt gccgtttatt taaatgaagg attaatgact gggatctttt tagaccaaaa
     3061 agaagtacgg ggatcattag cagaaggttt cgcttctggg aaaacagttt tgaacatgtt
     3121 tagttatacc ggcgcctttt ctgttgctgc cgcaatggga ggggcaatcg aaacaacaag
     3181 tgtcgatcta gcaaaacgca gtttaaaaaa aacacaggaa cagtttgaag tgaatggttt
     3241 ggatgtcgat cagcaaaaaa tcattgttat ggacgtattc gagtatttca aatacgccaa
     3301 aagaaaacaa tttttatatg acatgattgt attggatcca ccaagttttg ccagaaataa
     3361 gaaaaaagtc tttcgcgtag caaaagatta tggggaactg gtcaaggata gtctggatat
     3421 tttagcagaa gacgggatac ttattgcgtc tacaaatgct gcgaatgtct cgatagaaca
     3481 attccaaaca atgattgaag aggaattcca tgccaaaaat gtttcttttg aacaacttgc
     3541 tttatatcgt ctgccaaaag atttccagac gacggatacc tttttagaag gcaattatct
     3601 gaaagtgttt gtttatcaaa ttaaaaataa ccattagaag gagcgatgca gaatggttcg
     3661 ggtaaaggga ttgaatatag gaagcggtag accaaagatt tgtgcgcctc ttatcggggc
     3721 cgtccgagag gaagtattac gtgaagcagt tcttgcacgt gaagcagggg ccgatttagt
     3781 cgaatggcga gtcgatcatt atcgtgaagt tttttctttt gatgaagtag cagaaacatt
     3841 ggttcttatc catgagatgc ttgatggact gccgttatta tttacgtttc gtaccttagc
     3901 tgaaggcgga gaacaagaca tatcgatccg tcagtatcgc gacctttatg aacatgtgat
     3961 acacacagga ttagtagata tggtcgatat cgagttgttc aaagtagaaa gtctaggaaa
     4021 aagactgata gaggaaatca agtctttaaa cattccttta attatcagta gtcatgattt
     4081 taaagaaaca ccagcagacc ctgtcttgct ttaccgattg aatgttatgg aacattttgg
     4141 agccgatatc ggaaaactgg cagtaactcc caataatgaa cgagatgttt tgcggttgat
     4201 ggagttgact agacgggcaa atgcttttgt ctccatgcct attattacga tgtccatggg
     4261 aaatcttggt aaaatttctc gtctggttgg cggaacgacg ggttccgtta tgacatttgg
     4321 ttccttaagc agcgattcta cttctgctcc aggacaatta ccggtcaaag cattgaagca
     4381 aattattcaa ttattaaatg aaccgttgta gaacaagttt gaggtgatat tttttggcaa
     4441 agaaaaaaat agtaaaaacg aatgcaatcc gtatggtcga acaaaaaaag attccctata
     4501 cagagcacga atatgagtgg gacgaaagcc acctaagtgc gtcatcggtc gctgaacagc
     4561 ttccagaaag ccaatctcgt atatttaaaa cactggttgc tgtgggcaat gtgacggggc
     4621 ctcttgttgc tgtgataccg ggagaagccg aattgaatct aaaaaaatta gcaaaagttt
     4681 cagggaataa aaaagtagaa atgctccatt taaaagattt ggaagcaacg accggataca
     4741 tacgtggtgg ctgctcgcct ataggtatga aaaaactatt tcctacttat ttagatcaaa
     4801 ttgcagaaag ctatgaacaa atcattgtct cggctggcag aagagggcta cagatggagt
     4861 tagcacctca agatatctgt gcattaacga gcggacaatt tgcagatata aaacaataaa
     4921 tcatacgtat aaaagaaaga aggaggccgg gatgttgttt gtccagtctc cttctttctt
     4981 ttttatacat tatgctcttt gcccggcatt cattcctgct acatgccctg tagcgaaagc
     5041 cgctgtgata ttaaagccgc cagtataacc attgacatcc agtaattctc ccgcaaaata
     5101 cagaccatca atgattttac tctccaatgt tttaggataa atttccttta gagaaacccc
     5161 tccgccagtt acaaatgatt tctcaatagg aaaagttttg ttgattgata gcttaaactc
     5221 tttgcatagt tgaacaaaat cttgaatttg tttttgagag gcttgttgtc ctgtcagatg
     5281 atccatttca agccgctcaa gaaagaaaac taacaatcgc tcaggcaata taccatgcca
     5341 agcattgacc aagttctttt tggttgcttt acttttttct gtcaatatat gaataagctc
     5401 ttgtttggtt tgtgtaggga aacaatccag agaaagtgtc actggttctc ccgttttctc
     5461 taattcttta ttgacaaaac ttgaacaacg cagtgcagcc ggaccagaaa taccgaaatg
     5521 ggtgaaaagt aaatccatcg tatgttcagt caatacacga ccttttttat ttaaaacgcg
     5581 taaagtgatg tcttgtaaag aaagcccttg aagtgttttc tcctgaatat acggttcatc
     5641 cgatatcaag ggggactctg tcgcataaag aggcgtcacc gtgtgaccca ctcgcttcac
     5701 gatcttgtac ccatctccag tagaaccagt agatggataa gtccgtccac cagttgttaa
     5761 gataatggca ggagcatgga actcttcgaa ttccgtgcgt ataccgtaaa ttttatgttc
     5821 tttgtggatc aatttctcta ctcgagtaga gaatagaagc gtcacatcta gttctttcag
     5881 acgattgagc aatgcttcga taatcgtttt tgatttattt gttacaggaa acatccgtcc
     5941 atgatcctct tcttttaaat gtacgccttg tgattcaaag aagttcatga tatcgaaatt
     6001 attccattgc gcaaacgtac tatataaaaa ctttccattt ccaggaatat gtgcaatcaa
     6061 atcatcaaca ggacgattgt tcgtaacatt gcaccgacca ccacctgtca tcagtagttt
     6121 ttttcctgct tttttgtttt tttcgatcaa gagtacgcgt gcgccttgtt cagctgcact
     6181 gatcgctgcc atcatcccgc ttgttccagc gccgactacg atcacatcat acagtttttc
     6241 cattccctca cctcaaggaa agtgtagcat atctgaagaa agaaaacaga aaaaaatgtt
     6301 ttgagccaaa agagaaaatg agctaaaatg agggaagata agcaaaggag cgtggaacga
     6361 tgacaaacgc aacagaatat gttcaagcaa ttcaagaaaa gctacatcaa aaggatcaag
     6421 ggcaagaaga atttttacaa gcaatcgatg aattcatgcc cacggttatg tcttttttgg
     6481 acactcatcc cgaatatatc gaaaagaata tcttagaact tttaacggaa ccagaaagag
     6541 ttattcaatt tcgagtgcct tggcaagatg atgaaggaaa ctggcgagta aatagagggt
     6601 accgtatcca atacaactca gctatcggcc cttataaagg tggattgcgc ttccatccta
     6661 gtgtcaatct aagtatcttg aaatttttgg cttttgaaca gattttcaag aatagtttaa
     6721 ccggtctgcc aatcggtgga ggaaaaggcg gaagtgactt cgatcctaaa ggaaaatctg
     6781 ataatgagat catgcgtttt tgtcaaagtt tcatgctgga attgtcaaaa catatcggcc
     6841 catcgattga tgttcctgcg ggagatatcg gtgtaggagc tagagaaatc ggctatttgt
     6901 tcggcgaata taaacgattg aaaaaatatg atactggtgt gttgacaggt aaacctttgg
     6961 atttttgggg aagtaagata cgtacggaag ctactggtta tggattagtg tattatgtca
     7021 aacatctatt gaacgaagaa aaagattcat ttaatcaaaa aacagttttt atttccggaa
     7081 gcgggaatgt agccatttat gcaatcgaaa aagtgcaaga gctaggcggg aaagttgtta
     7141 catgttcaga ttctagcggg tacatctatg atccagaagg aatagatgtg aaactattga
     7201 aagaagtcaa agaaatcaat agacaacgtt tgacggaata cgcaaaccaa cgtccttctg
     7261 ctatttatca tgctggagaa tctgtttgga cattgaaaga aaaagcggat atcgctttgc
     7321 catgtgctac tcaaaatgaa atagacgaaa gcttggccga aatcttagta gaaaatggga
     7381 tcaaaatcgt agcagaaggc gctaatatgc catgtacagt aaaagctgtt tctgttcttc
     7441 atgaagcagg cgtgtggtat tgtccaggaa aagctgctaa tgctggagga gtagcagtat
     7501 ctgctttaga aatgagtcag aacgtccagc gattgacttg ggaaagagag caagtggatg
     7561 agcagctgga tcaaatcatg gaaacgattt accatacgtg tcgccaaaca gcagccgagt
     7621 acggcgacga aaaaaatctg cttcttggag caaatgttgc tggatttgaa aaagttgcca
     7681 aagcaatggc agcagaaggt ttagtttgaa aaatgaaact ttcttggaaa tattgtcagc
     7741 attttgttta tttgcttgaa aaataataaa aaaaaacgta aagtattacc tgtagagtaa
     7801 acctataagg aggagaacaa atggcacaca ttcattttga ctattcaaaa gtagcaccgt
     7861 ttgtcaacga acaagagtta gggtacatgc aaagccaagt aacagcagcc cacaacgaat
     7921 tacgggaagg tactggcgca ggaagcgact tccgcggctg gatcgattta ccaacgaact
     7981 atgacaaaga agaatttgca cgtatcaaag aagcagctaa aaaaatccaa tctgattctg
     8041 aagtattagt tgttatcggt atcggtggtt cttatttagg cgctcgtgca gcgatcgact
     8101 tcttgaatca cacattcttc aatttattag atagcaacac aagaaaagca ccacaaatct
     8161 tctttgccgg aaattcaatc agctcaacat atattgctga cttgatcgaa gtaatcggtg
     8221 atcgcgactt ttcagtaaac gttatttcaa aatctggaac aactactgaa ccagcgattg
     8281 cgttccgtgt attcaaagag cttcttgtta agaaatatgg acaagaagaa gcaaacaaac
     8341 gtatctacgc tacaacagat aaagcaaaag gtgcagttaa agtagaagca gatgcagaag
     8401 gttgggaaac tttcgtgatt cctgatgaca tcggcggacg tttctctgta ttaactgctg
     8461 ttggtttgtt accaatcgct gttagtggtg cagatatcga tgcattgatg caaggtgccg
     8521 ctgatgcaag caaagcatac tcaagcgaca aactagaaga aaacgaagct taccaatacg
     8581 cagcaatgcg caacattctt taccgtaaag gcaaagtaac tgagttgttg atcaactatg
     8641 aaccaggaat gcaatacttc tcagaatggt ggaaacaatt gtatggtgaa tctgaaggaa
     8701 aagaccaaaa aggcatctat ccttcaagcg caaacttctc aactgatttg cactctttag
     8761 gacaattcat ccaagaagga cgtcgcaata tttttgaaac agttgtgaag gttgaaaaac
     8821 ctcgtaaatc aatcacaatt cctgaacaag cagaagattt agacggtcta ggctacttgc
     8881 aaggaaaaga agtggacttc gtaaatacaa aagcatttga aggaacattg cttgctcata
     8941 cagacggtga cgtgcctaac ttgctagtca aaattccaga aatggatgct tatacattgg
     9001 gttatacaat gtacttcttt gaaattgctg taggtatttc aggttacttg aatggaatca
     9061 acccattcga ccaaccaggt gtagaagctt acaagaaaaa catgttcgca cttctaggca
     9121 aaccaggctt tgaagatctt gctaaagaat tgaacgaacg cctgtaagat aagggttttt
     9181 cgctagattt aaataatcag tgatagaaac atgttcgctt taaaatgaaa actggtaccc
     9241 atttaggtac tcaaacatac tgcatcgttt attcagaata aggggctgtg acaagacgtt
     9301 tgtcacagtc ccttgtttgt atagaaatcg attgattgcc ttgctcttta gtttgtttta
     9361 ggactttaag aacagtgagg ctgacttcga aggttaattc tgtttttttg ttttctagaa
     9421 tcagtaagca aaaaagcact cacttcgagt gcttttttgc gatatattct tagcttaaga
     9481 taggcctaat ttttctttta cagatgaggg taccttatcg ctggtaatta cttttttctc
     9541 tatgatccgc ttttcgcttg ctttcaactg caagatcgtt ccttcggggt aagcagaacc
     9601 attggcagag tcaccagaag taatgacatc gaaatctaac tgtctttttt caccgttttc
     9661 agcataagca tctacgttgt atttaaaacc tcttccaatt acttccccat tatctgcttt
     9721 gatatcagtt tctgcaggca ttggagcttt gactaccgca taataatctt tacctacata
     9781 agtagactga tagtagtccc aacctttcca tcctataaaa gcaatcaata tgataacgat
     9841 caaccctaaa atttttttca tgaaagatct ccttttttat ttttattata cccgtggtgc
     9901 tagttaattt taatatccta aagaatatct caaagttcct tcaaaacgtc gttgtgctcg
     9961 acttaataga acactgtaag ccagtaatat gctttcaaat ctactctcta gccctttaac
    10021 agaacgtgag ttgaaatttt gacaacctaa tttttctaag gaagaaaaaa cagtctcaac
    10081 tgt
//
LOCUS       DADVXU010000096         9940 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.100, whole genome shotgun sequence.
ACCESSION   DADVXU010000096 DADVXU010000000
VERSION     DADVXU010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.100"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            32..388
                     /locus_tag="KB953_002331"
     CDS             32..388
                     /locus_tag="KB953_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-rich SFCGS family protein"
                     /protein_id="HBB6867269.1"
                     /translation="MIKVVIADRMGKGQNVAKGVEAAGGKAVVVPGMGADMRLGDVMQ
                     QEHADMGISFCGSGGAGALTAATKYGYPERHGMRSIEEGITAIKDGKTVLGFGFMDQE
                     ELGKRLTEEFIKKHGK"
     gene            404..754
                     /locus_tag="KB953_002332"
     CDS             404..754
                     /locus_tag="KB953_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4312 family protein"
                     /protein_id="HBB6867270.1"
                     /translation="MSVQKTIQQKVIVSGKGETKQHAFASALAEIQNKIMEQSNVLLR
                     IEPVNVDILKAVENKYTERFLFFFFPRQRISYEVELSVTVAVTEIKLADISFSQQMTE
                     DPNGIAIPFVTKKI"
     gene            783..1562
                     /locus_tag="KB953_002333"
     CDS             783..1562
                     /locus_tag="KB953_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4311 domain-containing protein"
                     /protein_id="HBB6867271.1"
                     /translation="MDFVIILVKSVIIGGLLGFAASVGAARMFHAPNTQGLGAFRTLG
                     EMNACMGDPASHFSFGLGFFFNAWASSVGAGAFTQDVTHRIIPNWAAAALLAKNKDVT
                     QTMHDPKKMGIIGAVIGAVVVAFLNTTAAAIPEALQVTAVSVLVPAATILINTVMPVI
                     FWLAALDAGKRTGFWGTLFGGIAQLIMGNAVPGVVLGILIGKGVDESGWNRVTKSMMI
                     AIVLLFIFSGFFRGFDMEMIKSFQLQVPNWLDNLHNIFSVK"
     gene            1573..2235
                     /locus_tag="KB953_002334"
     CDS             1573..2235
                     /locus_tag="KB953_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4310 family protein"
                     /protein_id="HBB6867272.1"
                     /translation="METTEEQAKVLEAKRQKSFWFADWSFPLIVGIMSAAVFAGTHMY
                     VMYGVGAFNEVSIVAMLKAGLDGGSYGAAAAFGASFLFARILEGSLVGILDLGGSILT
                     GIGIGVPAILLSMKIAAPIDNFALSLLTGLVLGLIVGGIIVLIRKFTINQSNSTFGAD
                     VMMGAGNSSGRFLGPLIILSACAASIPIGIGATLGALGFYAWKKPIAGGAILGAIFPA
                     VN"
     gene            2254..3360
                     /locus_tag="KB953_002335"
     CDS             2254..3360
                     /locus_tag="KB953_002335"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HBB6867273.1"
                     /translation="MFDTLIKNGRLVDGSKIEVAIEKGMIKAVAAEINQPAQEVVDLE
                     GKYYLSAGWIDDHVHCYEKMNLYYDYPDQIGVEKGVTTVIDAGTTGAENIREFYQLTK
                     SVKTNVYALMNISKWGIVKQDELADLTKIQEDLVSTALAELPEFIVGIKARMSKTVVG
                     ENGIKPLELAKKIQTENNDLPLMVHIGSAPPELEEVLSRMERGDVLTHCFNGKPNGIL
                     DPKTDQIKNFVWDAYNNGVVFDIGHGTDSFNFHVAETALKAGMKATSISTDIYIRNRT
                     NGPVFDLATTLEKLRVVGYSWEEIIEKVTAVPARNFHLKNKGQLAVNYDADLTIFEIV
                     ENEKELKDSNGFTRIAKEQILPIKTIIGGVIYDN"
     gene            3350..4441
                     /locus_tag="KB953_002336"
     CDS             3350..4441
                     /locus_tag="KB953_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HBB6867274.1"
                     /translation="MTISYGKFDLKEVINASGKMTILGVSKVSESVLAAQRFGGEHFF
                     EMSDLAIKTGAYLSQLLKVEDAQIVSSASAGIAQSVAALIGQGDMYHVYHPYTEKIAR
                     REIIIPKGHNVDYGTPVEVMVAQGGGKVIEAGYANMCTPEHVSMMITEKTAALLYIKS
                     HHAVQKSMLTVAEMVEVAKAHRLPLIVDAAAEEDLFKYSEMGVDLVIYSGAKAIEGPS
                     AGLVIGKKKYIQWIRLQSKGIGRAMKIGKDNILGFTQALEDYLKNGSESGDSMKQRLA
                     PFVNAINQLPDLSAKIVQDGAGRDIYRASVTINGDKSAKEVIQELKARNPAVYTREYQ
                     ANNGIIEFDIRSVSQNEMEKIIKRLREIM"
     gene            4454..5194
                     /locus_tag="KB953_002337"
     CDS             4454..5194
                     /locus_tag="KB953_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HBB6867275.1"
                     /translation="MSVIPNYYKNRVCLNVLAHSVENAKACYEAAEGHVVLGVLSKNY
                     ASDEAAIEDMKRYQDATNNALSVGLGAGDPNQSQMVSRLSGVLQPQHVNQVFTGVGTS
                     RALLGQNDTVINGLVSPTGKIGYVNIATGPLSSQAPAAEVPIETAIQMLQDMGGTSIK
                     YFPMKGLAHREEFKAVAEACAKYEFYLEPTGGIDLSNFEEILKIAIDAGVKKVIPHVY
                     SSIIDKETGDTRIGDVKILLSMVKKIIH"
     gene            5482..7422
                     /locus_tag="KB953_002338"
     CDS             5482..7422
                     /locus_tag="KB953_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HBB6867276.1"
                     /translation="MKKIIAITSCPVGIAHTYMAAENLEKAGKAVGAEVKVETHGSIG
                     IENELTARDIEEAAGVIIAADTKIDKSRFGGKPLITVGVQEGIHHADELVRDILAGKA
                     PVYKSTEAIISSENKSESENLGKKIYKSLMNGVSYMVPFVVTGGLLIAISLTLGGTAT
                     PEGIKIPEGTIWATMNSIGSIAMGLMVPILSAFIAQSIADRPGLVPGFVGGMLAANGA
                     LYGSDANAGFLGGIITGFLAGFIALGIKKVRVPKALQSIMPIIVIPILGSLAVGFVFI
                     YVIGEPVALLFSSLTHFLAGMQGGSEIVLAMILGAMIAFDMGGPFNKVAFLFGSGLIA
                     EGNYSIMGPIAVAICIPPLALGLASLILKTKFTKTEREAGKASLAMGLFGITEGAIPF
                     ASTDPLRVIPSIVIGAMTGSVIAMLSGVTDHVAHGGPIVAVLGAVDHVAMFFVAVIAG
                     IAVTVLMLRILKPTLQQTAVAAANGVTVADLSETETSDVYTKTETFDSITDIMSDEAI
                     VLGETATTKDGVIEDLVQRLEKTAAITDKTRFKEVIYDREHESTTGIGMGIAIPHGKS
                     AYVLKPTVAFARSQSGVDWHSLDGKPAHMIFMIAVPENSQGDMHLKILQRLSRKLMDD
                     DFRQALMEAPDKTTVHQLLSEM"
     gene            7509..9431
                     /locus_tag="KB953_002339"
     CDS             7509..9431
                     /locus_tag="KB953_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBB6867277.1"
                     /translation="MNQRQQSLIYQLSENNNYITTKKLAEHFHVSERTIHSDLNTIET
                     WLRDKHLPLMFERKKGTGILLNGLPHEREQLKRALNEETQTEMDKKQLRQLLIFHLLV
                     AKDGFSLEELSEKLYVGKRTIRKELTELQSFFEYHHLQLVSKTKLGTFLKGDEQEKRQ
                     LLVKTLRNMQKEDPQSPSLKEFFAKDTLKVIQHTLQDVFYENHLEQPNGLASVEIHIY
                     FMLERMKQYQKVKLSKDENEVVEHTQAQKLSSQILAKLATIYPIEFSPDEINYLALRI
                     ANLFTNSQAATRFQKESSALADHLIEQVEQFLGYSLKEDQLLKQNLRSHLSSTYFRLH
                     YGLQISNPLTKNVFSTYTQLFLVLQLILEDYFEKENFYVPQEETAYLTIHFQAAIERH
                     KHQKSRRYQTVLVSEYSKAMATFLEARLNRELPELTIMDLVEYKPELKRQDFPSVDFI
                     LATLPFKHISIPVIEISPMITETDLAYLTKYMLEHVPIKKKKTFDLASFTHPFLIFPQ
                     LERTDPADILNFMGNVLVEHHYVEPEFIDSVLERDSHSSTRVAPFVTIPHGNPLYVKH
                     SMISIATMKEPVLWHGEQIRIVLLLAIKKNDLKHAEFKKIFSVIHYLEKQPEQMAQLM
                     QSNHPLEILTLLSSYE"
     gene            complement(9711..>9940)
                     /locus_tag="KB953_002340"
     CDS             complement(9711..>9940)
                     /locus_tag="KB953_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HBB6867278.1"
                     /translation="IDRDYFTQADLIITLCGDAKDKCPAVPNNHEHLHWDLKDPAQAV
                     GSEEEILTEFRKVREGIKRKVLDLNQRTSIV"
BASE COUNT         3245 a         1650 c         2207 g         2838 t
ORIGIN      
        1 acaaatgata ttttacgagg aggaaacaat catgattaaa gtagttattg cagaccgtat
       61 gggtaaagga caaaatgtgg ccaaaggggt agaagcagct ggtggaaaag cagttgtcgt
      121 accaggaatg ggggcagata tgcgtttggg cgatgtgatg caacaagagc atgcagatat
      181 gggtatctct ttttgtggta gtggtggtgc aggtgcatta acagcagcaa caaaatatgg
      241 ctatccagaa cgacatggta tgcgttcgat tgaagaaggg atcacagcta tcaaggatgg
      301 caaaactgtt ctaggtttcg gctttatgga tcaagaggaa ttagggaaac gtctgacaga
      361 agaatttatc aaaaagcatg gaaaatagaa agagggacat gaaatgagcg ttcaaaagac
      421 aattcagcaa aaagttatcg tctccggtaa aggggaaaca aagcaacatg cttttgcatc
      481 agcgttagca gaaattcaaa acaaaataat ggaacaatca aacgtattat tgcgaatcga
      541 accagtcaat gtggatattt taaaggcggt cgaaaacaaa tacacagaac gctttctatt
      601 tttcttcttc ccacggcaac gtattagcta tgaagtcgaa ctctctgtta ctgtggctgt
      661 aacagaaata aaattggcag acatatcgtt cagccagcaa atgactgaag atccaaatgg
      721 gattgccatt ccatttgtaa ctaaaaaaat ctaattttga atcataaggg ggaaagaata
      781 acgtggattt cgtaatcatt ctagtcaaat cggtcattat cgggggatta cttggttttg
      841 cagcatctgt cggggcagct cgaatgtttc atgcacctaa tacacaaggt ttaggagctt
      901 ttagaacatt aggcgaaatg aatgcctgca tgggtgatcc agcttctcat ttttcatttg
      961 gtttaggctt tttctttaat gcatgggctt cttcagttgg tgctggtgca ttcacacaag
     1021 atgtgacgca tcgaatcatt cccaattggg cagcggcagc acttctagca aaaaacaaag
     1081 atgtcaccca gaccatgcat gatccgaaaa aaatggggat cattggagcg gttatcgggg
     1141 cagtggtggt agccttctta aacacaacag ctgcagcaat tccagaggcg ttacaagtga
     1201 cggcggtttc cgttttggta ccagcagcga cgattctgat caatacagta atgccagtta
     1261 ttttttggtt ggcagcttta gacgcaggga aacgaactgg tttttggggc actctttttg
     1321 gtggtatcgc gcaattgatt atgggaaatg cggtacctgg tgttgttcta ggaatcttga
     1381 ttggtaaagg cgtcgatgag agcggttgga atcgcgtcac gaagagcatg atgatcgcta
     1441 ttgttttatt atttattttc agtggatttt tccgtggatt tgatatggaa atgatcaaga
     1501 gcttccagct tcaagtgcct aattggcttg ataacttgca caacatcttt agtgtgaaat
     1561 aggaggagaa tcatggagac aacagaagaa caagcaaaag tattagaagc aaagcggcaa
     1621 aagagctttt ggtttgcaga ctggtctttt ccgctaatcg taggaattat gtccgctgct
     1681 gtatttgcag gaacacacat gtatgtgatg tatggtgtgg gtgcattcaa tgaagtttcg
     1741 atcgtggcga tgttaaaagc cgggctggat ggcggatcct atggcgcagc ggcagcgttt
     1801 ggtgctagtt tcttatttgc cagaatttta gaaggctctt tagttgggat cttggactta
     1861 ggcggttcga tcctaactgg gatcggaatt ggggtaccag caattctctt aagtatgaaa
     1921 atagcggcac caatcgataa ttttgcactc tctttattga ctggtttggt tctaggtttg
     1981 attgttggtg gcatcattgt cttgattcgt aaatttacga tcaatcaatc aaattccact
     2041 tttggtgctg acgtcatgat gggggcagga aattcatctg gacgtttttt gggtccatta
     2101 attatattaa gcgcgtgtgc agcatctatt ccaatcggca ttggcgctac attaggagca
     2161 ctaggattct atgcttggaa aaaaccaatt gctggaggtg ctattttagg agcaatcttc
     2221 ccggcagtaa attaatagaa agagggtcat taaatgtttg atactttgat taaaaatgga
     2281 aggttagttg atggatctaa aatagaggtc gctatagaaa aaggcatgat taaagcagtt
     2341 gcagcagaaa taaatcagcc ggcgcaagaa gtagttgact tggagggaaa gtattattta
     2401 tccgctgggt ggatcgatga tcatgtccac tgctatgaaa agatgaatct ttattatgac
     2461 tatcctgatc agataggggt cgaaaaaggt gtgacgacgg tcattgatgc tggaactacg
     2521 ggagcagaaa acatccgtga attttatcaa ttgacgaaat cggtcaagac aaatgtgtat
     2581 gcgctaatga atatctctaa atggggcatt gtcaaacaag atgaacttgc tgatctaaca
     2641 aagattcaag aagatctagt aagtactgcg ttagcagaac tgccggaatt tattgtcggg
     2701 attaaagcga gaatgagtaa gacagttgtt ggtgaaaatg gcattaagcc attagaatta
     2761 gctaaaaaaa tccagacaga aaacaatgat ctgccattga tggttcatat cggttctgca
     2821 cctcctgagt tagaagaagt attgtcaaga atggaaagag gcgatgtatt gacccattgt
     2881 tttaatggaa aaccaaacgg tattttagat ccaaagacag atcaaattaa aaactttgtc
     2941 tgggatgctt acaataatgg tgtcgtcttt gatattggac atgggacgga tagctttaat
     3001 ttccatgtgg cagaaaccgc actaaaagca ggaatgaaag ctacctctat tagtacggat
     3061 atctacatcc gcaatcgtac aaatggccca gtttttgatc tagcaactac tttagaaaaa
     3121 ttgagggtag tcggctattc ttgggaagaa atcattgaaa aagttacagc agttcctgca
     3181 cggaattttc atttgaagaa taagggccag ttagctgtca attatgatgc agatttgacg
     3241 atatttgaga ttgttgaaaa tgaaaaggaa ttgaaagatt ccaacggatt tacacgtata
     3301 gctaaagaac aaattttacc gattaaaaca attattggag gcgtcatata tgacaattag
     3361 ctatggaaag tttgatttaa aagaagtcat taatgcatcg ggaaaaatga cgatcctagg
     3421 ggtttctaag gtatctgaaa gtgtcctagc agcacaacga tttggaggcg agcatttttt
     3481 tgagatgagt gatcttgcca tcaaaaccgg cgcttatctt tctcaattac tgaaagtgga
     3541 agatgcgcaa atcgtttctt ctgcttcagc agggattgct caatctgttg ctgctttaat
     3601 cggacagggc gacatgtacc atgtctatca tccctataca gaaaaaatcg ctagacggga
     3661 aatcatcata cctaagggac ataatgtaga ttatgggaca ccggttgaag taatggttgc
     3721 tcaaggtgga ggaaaagtca tagaagctgg ctatgcaaat atgtgtacac cggaacatgt
     3781 ttccatgatg attacggaaa aaactgctgc gcttctatat attaaaagtc atcatgcagt
     3841 tcagaaaagt atgctgacgg tggcagaaat ggtagaagtt gccaaagctc atcgtttgcc
     3901 attgatcgtt gatgcagctg cggaagaaga tttattcaaa tatagcgaaa tgggcgttga
     3961 cttagtgatt tactctgggg caaaagcaat cgaaggtccg agtgccggac tggtcatagg
     4021 aaaaaagaag tatattcaat ggattcggct acagtctaaa ggtattggcc gagcaatgaa
     4081 aatcggtaaa gataatattt taggctttac ccaggcacta gaagattatt taaaaaatgg
     4141 gagcgaatcc ggtgactcca tgaagcaacg attagcgcct tttgttaacg caatcaatca
     4201 acttcctgac ctttcagcga aaatagttca agatggtgcc ggcagagata tttatcgagc
     4261 aagtgtaacg attaatggcg ataaaagtgc gaaagaagtc attcaggaat taaaagcacg
     4321 aaatccagca gtctacaccc gagaatatca agcaaataac ggaatcattg agttcgacat
     4381 tcgttccgtc agccaaaacg aaatggaaaa aattataaaa agattgagag aaatcatgta
     4441 ggaggctttt aaattgtcag ttatcccaaa ttattataaa aatcgtgtat gtttaaatgt
     4501 attagctcat tcagttgaaa atgcaaaagc atgctatgaa gcagcggaag ggcatgtcgt
     4561 actaggtgtg ctatcgaaaa attatgctag cgatgaagca gcaatcgaag atatgaaaag
     4621 gtaccaagac gcaaccaata atgcgttgtc agttggatta ggcgctggag atccaaacca
     4681 aagtcagatg gtttcgcgtt tatctggggt tttacaacca caacatgtca atcaagtatt
     4741 tactggcgtg gggacttctc gtgctttatt gggccaaaat gatacagtca tcaatggatt
     4801 ggtttcacca acaggtaaga tcggctatgt caatattgct acaggtccgt taagttcaca
     4861 agcgccagca gctgaagtgc ctattgaaac tgccattcaa atgctgcaag acatgggagg
     4921 tacttcaatc aagtatttcc cgatgaaagg cttagcacat agggaagaat ttaaagcagt
     4981 tgcggaagct tgcgcaaaat atgaattcta tttagagcca acaggaggaa ttgatctttc
     5041 aaactttgaa gaaattctga aaattgcaat cgatgcaggt gtaaaaaaag tgattcctca
     5101 tgtttatagt tcaattatag ataaagaaac aggagatacg cgtataggag acgtaaagat
     5161 actattaagc atggtaaaaa aaattattca ctaactcatt agaaaataag aagcttattc
     5221 cagaatgaaa ttaaatggaa taagcttttt ataattaatt acggaaattg ttaatgtaga
     5281 taataattta gatgtacgta agcagaagta cagaaatgta agattttcca atattatttt
     5341 attcaacata ataaatcgta tatcaatttt tttagcgaaa tttctattta ttccaagaat
     5401 ttcagttatt acggaatcat tttgtgctta ataaaaagaa accgttttta tatactataa
     5461 atgtagaaag gcggtaggaa aatgaaaaaa attattgcaa tcacatcctg tccagttggt
     5521 attgcccata catacatggc agcagaaaac ttagagaaag cggggaaagc agttggcgcc
     5581 gaggtaaaag tcgaaacaca tggttccatc ggtattgaaa atgaattgac tgcaagagat
     5641 atcgaagaag cagctggtgt aatcatcgca gcagatacta aaatagataa atcacgattc
     5701 ggcggtaaac ctttgataac cgtaggtgtg caagaaggaa tccaccatgc agatgagttg
     5761 gtgagagata ttttagcagg aaaagcgcca gtctataaaa gtacggaggc aattatttct
     5821 tccgaaaata aaagtgaatc agaaaattta ggtaaaaaaa tctataagag tttgatgaat
     5881 ggcgtttcgt acatggtacc tttcgtagtt acaggagggt tattgatcgc gatttcttta
     5941 acattaggag gaacagctac acctgaaggg atcaagatac cagaaggaac gatttgggca
     6001 acgatgaata gtatcggaag tatcgctatg gggttgatgg ttccaattct atctgcattt
     6061 attgcgcaaa gtattgcaga tcgtccagga cttgttcctg gatttgttgg tgggatgtta
     6121 gcggcaaacg gagcattata tggtagtgat gcgaatgcgg gattcttagg cgggatcatc
     6181 actggatttt tagcaggatt cattgcttta ggaatcaaga aagtgcgagt accgaaagct
     6241 ttacaatcga tcatgccaat tattgttatc ccaattttag gatcattggc agttggtttt
     6301 gtgtttatct atgttattgg agaaccagta gctcttctat ttagttcatt gactcacttt
     6361 ttagcaggga tgcaaggagg aagtgaaatt gttctagcta tgatcttagg agcgatgatt
     6421 gcttttgata tgggggggcc gttcaataaa gtagcctttt tgttcggatc tggcttgatt
     6481 gcagaaggca attattccat tatggggcca attgctgtag ctatctgtat ccctccatta
     6541 gcattggggc tggcatcatt gattttaaaa accaaattta cgaaaacaga acgagaagct
     6601 ggtaaggcgt ccttagcaat gggactgttt gggattacag aaggcgctat tccgtttgct
     6661 tcaacagatc cactacgggt gattccaagc attgtaatcg gggcaatgac aggaagtgtg
     6721 atcgcgatgc tttcaggagt gacagatcat gtagctcatg gcggtccgat cgtagcggtt
     6781 ttaggagcag tggatcacgt agccatgttt tttgtagcag tgattgctgg tattgctgta
     6841 accgtgctaa tgttaagaat ccttaaaccg acattacaac aaactgcggt tgctgcagca
     6901 aatggtgtga cagtagcaga tctatcagag actgaaacaa gtgatgttta tacgaaaact
     6961 gaaacttttg attctatcac ggatattatg tcagatgaag caatcgtttt aggtgaaaca
     7021 gcaacaacaa aagacggagt aatcgaagat ttagtccaac gtttagaaaa aacagcggcg
     7081 atcacagata aaaccagatt taaagaagtc atctatgata gagaacatga aagtacaacg
     7141 ggaatcggta tggggattgc tattccacat ggaaaatcag cttatgttct taaaccaaca
     7201 gtggcatttg ccagaagtca atctggggta gattggcatt ctttagatgg aaaacctgca
     7261 catatgatct ttatgattgc tgtacctgaa aacagccaag gtgatatgca cttgaaaata
     7321 ctgcaacgtc tatcacgtaa attgatggat gatgacttcc gtcaagcact gatggaagcg
     7381 ccagataaaa caacagttca ccaattatta agcgaaatgt aacataagat gaaaaatcga
     7441 ccgtgatgag acataataga tgtgttatca tgatcgattt tttatttaga agggagtttc
     7501 tgcacattat gaatcagcgt cagcaatctt tgatctatca attaagtgag aacaataatt
     7561 atataacaac aaaaaagcta gcagagcatt tccatgtgtc agaaagaacg attcatagcg
     7621 atttgaatac cattgaaact tggttgagag acaaacatct gcctttaatg tttgaaagaa
     7681 aaaaaggtac aggtatcttg ttgaatggtc tgcctcacga aagggaacaa ctgaaacgag
     7741 cgcttaatga agagactcaa acagaaatgg ataaaaaaca gcttcgtcag ttattgattt
     7801 tccatctgct agtagcaaaa gacggattta gtcttgaaga actttcagaa aaactatatg
     7861 ttggaaaacg tacgattcgt aaagaattga cagagttgca aagctttttt gagtatcacc
     7921 atttacagct agtatccaaa acaaaactag gaacttttct taaaggcgat gaacaagaaa
     7981 aaagacagtt gcttgttaag acattacgaa atatgcagaa agaagatcca caaagtccgt
     8041 cgttgaaaga attcttcgct aaagatacat taaaagttat ccaacataca ttacaagatg
     8101 ttttttatga aaaccattta gaacagccga atggactggc tagtgtggag attcacatct
     8161 actttatgct cgagcgaatg aaacagtacc agaaagtgaa attaagtaaa gacgaaaacg
     8221 aagtcgtgga acacacccaa gcacaaaagt taagtagtca aatattagca aaactagcca
     8281 ccatctatcc aattgaattt tctcctgatg aaatcaatta tctggctttg cgtatcgcca
     8341 atctttttac aaattcacaa gcagccacac gttttcaaaa ggaatctagc gcacttgctg
     8401 atcatttgat cgaacaagtg gaacagtttc taggctattc attaaaagaa gatcagctac
     8461 taaaacaaaa tttaaggtct catttatctt caacttattt tcgactccat tatggtttgc
     8521 agatttctaa tccattgacg aaaaatgtgt ttagtacgta cacacaactt ttcttagttt
     8581 tacagttaat tttagaagat tattttgaaa aggaaaattt ttatgttcca caagaggaga
     8641 cagcttattt aacgatccat ttccaagcag cgatcgagcg acacaaacat cagaaaagta
     8701 gaagatatca aacggtttta gttagtgaat attcaaaagc tatggctact tttctcgaag
     8761 cacggctgaa cagagaactt ccagaattaa cgattatgga tctagttgaa tataagccag
     8821 aattaaagag acaagatttt ccctctgttg attttatctt ggctacgtta ccatttaaac
     8881 atataagcat tccagtaatc gagatctcac caatgatcac agaaactgat ctagcctatt
     8941 tgacaaaata tatgctggag catgtgccta tcaagaagaa aaaaacattt gatctagcaa
     9001 gctttactca tccatttctg atctttcctc aacttgaacg gacagaccca gccgatattt
     9061 taaactttat gggaaacgtc ttagtggaac atcattatgt ggagccggaa ttcatcgatt
     9121 ctgtcttgga aagagacagt cactccagta ctagagtagc accatttgta acaatccctc
     9181 acggtaatcc gctgtatgtg aagcattcga tgatttctat tgctacaatg aaagaaccag
     9241 tcctatggca cggggaacag atacgtatcg tactgctatt agcaataaag aaaaatgatt
     9301 tgaaacatgc agagttcaaa aaaatatttt ctgttattca ctatctagaa aagcagccag
     9361 aacaaatggc tcagttgatg caatccaatc atccgctaga aatcttaacg ttgttatcta
     9421 gttatgaata agtagcgatt aaaaaactgc ctttatattt ataataattc tatctattta
     9481 tttggaagta gaaaactaaa tacaaaaaaa gaggatgtgg ccaaagttgt ggcacatcct
     9541 tttattttga gtttcttata attttttata gttgcttacg atgtcttgac tactggtaaa
     9601 cggtgttcaa gagacaattt gtcggcaata aggcacaatt ttgtaaaata tggaaagcta
     9661 tttttttaac attgtttctt agtgcttgaa aaatcatttt gaatacttct ttatacgatc
     9721 gatgtacgtt gatttaagtc taaaactttt cttttgattc cttctctgac ttttctgaat
     9781 tccgttaata tttcttcttc gctcccgaca gcttgtgcag gatcttttag gtcccagtga
     9841 aggtgttcgt gattgtttgg aacggcaggg cacttgtctt tggcatcacc acaaagtgta
     9901 atgatcaagt ctgcctgagt aaagtaatct ctatcaatca
//
LOCUS       DADVXU010000097         9554 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.101, whole genome shotgun sequence.
ACCESSION   DADVXU010000097 DADVXU010000000
VERSION     DADVXU010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9554
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.101"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            118..1107
                     /locus_tag="KB953_002341"
     CDS             118..1107
                     /locus_tag="KB953_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBB6867279.1"
                     /translation="MRDKEMRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAG
                     QYLFDKELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGA
                     MTICLSNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWET
                     CLNEFDKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFME
                     MQWINSNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVL
                     DQADFDLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1089..1550
                     /locus_tag="KB953_002342"
     CDS             1089..1550
                     /locus_tag="KB953_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBB6867280.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1600..2064
                     /locus_tag="KB953_002343"
     CDS             1600..2064
                     /locus_tag="KB953_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBB6867281.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2078..2827
                     /locus_tag="KB953_002344"
     CDS             2078..2827
                     /locus_tag="KB953_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBB6867282.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2827..3657
                     /locus_tag="KB953_002345"
     CDS             2827..3657
                     /locus_tag="KB953_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBB6867283.1"
                     /translation="MENSTEKKRLITKKDLNKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTSFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3657..4304
                     /locus_tag="KB953_002346"
     CDS             3657..4304
                     /locus_tag="KB953_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBB6867284.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4402..5178)
                     /locus_tag="KB953_002347"
     CDS             complement(4402..5178)
                     /locus_tag="KB953_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBB6867285.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLAESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            5322..6281
                     /locus_tag="KB953_002348"
     CDS             5322..6281
                     /locus_tag="KB953_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBB6867286.1"
                     /translation="MTYTHLTTDELVIIESYFNIEKPVALVAQSLNRSRQTIYNVYSF
                     LKQGKKVLDYYRQYKQNKSRCGRRSTVLSDEQSTYVQEKVAQGWSPDVIIGRGEFPID
                     CSVRTLYRMFKKGLFDTQSLPMKGKRKPNNHQEKRGKQAFRRTIHERNSLYPHLTKEF
                     GHLEVDTIVGVKHKSAVITLVECLSKVIITLKPTGRRAEDIENRLNDWFKSMPKHLFK
                     SMTFDCGKEFSNWRSISNSNDIAIFFADPGTPSQRGLNEHSNGLLRRDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            6398..6706
                     /locus_tag="KB953_002349"
     CDS             6398..6706
                     /locus_tag="KB953_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HBB6867287.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQFHLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEARKEYLISPKLRLKSVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            6733..7032
                     /locus_tag="KB953_002350"
     CDS             6733..7032
                     /locus_tag="KB953_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HBB6867288.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGMLPEDVT
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTNE"
     gene            7206..7757
                     /locus_tag="KB953_002351"
     CDS             7206..7757
                     /locus_tag="KB953_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HBB6867289.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFPRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            7903..8196
                     /locus_tag="KB953_002352"
     CDS             7903..8196
                     /locus_tag="KB953_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867290.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            8189..8485
                     /locus_tag="KB953_002353"
     CDS             8189..8485
                     /locus_tag="KB953_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867291.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHAGYRHSWERIN"
     gene            8556..>9554
                     /locus_tag="KB953_002354"
     CDS             8556..>9554
                     /locus_tag="KB953_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HBB6867292.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKETVETDTTIEAKKSKTQS
                     TEQTTTEMKQSVEASTSESSIIESSSEEVKNTLWDSEKSTQLAIFVPQWGKTLGQEYK
                     SYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVET
                     GEYLGQHVYFFGFQNGQPKVYLTQQNQGNDKNYMYFNETQNQQLKQGFIDIVNGQTPQ
                     TPVVEESTQQATTTTTQSTSSSAISSWEAAKETVLNNKALWLYDSPDKNASNTNLIST
                     NEYMKDLMQDENGSYYSINIVTDDNGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMI"
BASE COUNT         3378 a         1369 c         1790 g         3017 t
ORIGIN      
        1 attttattat ataaaattgt tataaaaaaa tttttttgaa aaatagtatt gcaaaataaa
       61 gcaatttcat agataatagg tattgtaaag ttatatgact ttataatact tattagagtg
      121 agggataaag aaatgagaca actacacaaa gaacagattg aaacagcaat ggcagcgtta
      181 aaggaaaaga caattgatca tgtatatttt acagcatgtg ggggttcaca agccgtttta
      241 atggctggtc aatatctatt tgataaagaa ttaactgtac cttgtcatgt ttatactgct
      301 aacgaattta actatgctat accaaaagga ttatctgaaa attctttagt tatcacttgt
      361 tcccattctg gcacaacacc agaaactgta gaagcaacaa aaaaagcaag agaatttgga
      421 gcaatgacta tttgtttatc taatgaagaa ggatctcctc tatgggaagc agctgaatat
      481 cctgttcact atgattgggg taaagatgtt gatgctagtg atttaaataa aggaattctt
      541 tatggtttat tatttaatat tttacttgtt ttagatggct ctaaaaaatg ggaaacttgc
      601 ttgaatgaat ttgataagtt aaatgatctt gttattgctg aaaagagcaa atttgaaata
      661 actgctaaag aatggggtaa aaaccaaaaa cgagataaaa ttatttacac aattggtagc
      721 ggtatcaatt acggagaaat ttactccaca gcagcttgct ggttcatgga aatgcaatgg
      781 attaattcta atgctattca ttctggggaa tttttccatg gtccttttga aatcactgat
      841 tatgatgtac cattcatctt agtgaaaagt attggagata caagattttt agatcaacgg
      901 gtagaagact tcactaaaaa attcagtaat aaattaatgg ttttagacca agctgatttt
      961 gacttagaat ctatagatag tgtagcgaaa gaatatattg cagctgtact tagtggcgtt
     1021 gttattcgcc aaatggtaga agcgattgct tttgaaagag gccattcctt agatgttaga
     1081 cgctatatgt ggcaaatgaa atattaatta ataggaggtt gcaaaagatg gcaaatcaat
     1141 tatttcacat tgtgattaca acacattcta ccctttgtga gggttattta aaggcaacaa
     1201 aattaatttt aatgaatgac gccacaggta ttagtgctgt tccatttaaa gaaaatacta
     1261 gtccagatga ttttgaggag agtatgaaag aaataattag tcagcatcat ttgcaacctc
     1321 taattatttt aacggattta attggaggaa cacctaataa tgtttctatt aaacagttga
     1381 acaacaatag aattcaaatt attgctggga ttaatttacc tcttttactg gagttattaa
     1441 tggcccagtc agctggtaaa tctatagaag aaatagattt taaggaatta ctgaataact
     1501 ctcaaaaaag tatggttcat attaattcgc ttttaaaaga tgtcagttaa ggtggcaatt
     1561 tctattctat aaattttaaa aattgaagga ggaaatagaa tgattaaatt agtgcgtgta
     1621 gaccacagat tacttcatgg acaagtaata ttttcttgga ctaaacagta tgatattaac
     1681 catattattg tggctgataa caaagttcca ggagatccaa tgtctgtgat ggctttgaca
     1741 attgctaaac ctgctgattg caaattagac attgttaaaa ttagtgatgt taaagggatt
     1801 atggaaacat ccccaaatga taattacatg attttaatta aaggacctaa ggaagctttt
     1861 gaattagttc aaaatttacc agaaattaaa gaaattaatt ttggcggtgt gtctaagaaa
     1921 agcgagtcca aacaatatgg aaaggcagtc tttttaactc ctgaagaatt agaagccaca
     1981 aaaaaaattc ttgatcaaga agtgaaaata tttgtgcaac aagtcccatc ttcaagtatt
     2041 gaaaatgtag attttagtaa gtaagggggg aaaataaatg ttattaaaag ccattttatt
     2101 aggtttagtt ggtgtggtag ctgttattga ctccaggtta attggtcgac aaaatattgg
     2161 tagaccacta attttgagta cacttgccgg acttgtgcta ggtgacttgc atacagggat
     2221 tgttcttggg gcatctttag aattaatttc tatgggattc gtctcaattg gggcggctgg
     2281 acctcctaat atgcaacttg gtagtattat tgccactgct tttgcaattt taactaatag
     2341 tagtacagaa acagccttaa caatagctat cccagtggct gtagctggtg aatttatgag
     2401 tattatcatg cgcatgataa ttgctcaatt tggacatgct gcagataaag ccattgaaaa
     2461 taataaattt agaaaagctc aaattattca tatttattgg tcatttattt ttaatgcttt
     2521 tgtttacttt attccaatct ttttaacagt atattttggt gctgacgttg tggcagattt
     2581 agtagataaa attccagaaa tgattacaga cgccttgact gtctctggaa atatgctttc
     2641 cgcattaggg tttgcaatgc tattaagcac aatgttgagt aaaaaattat atccttactt
     2701 tatttttgga ttctttattg ttgcctattc aggtctttca ctaattggcg tgacaatttt
     2761 tgcagctctg attgcctttg taatggatca agttaaatat ggaaaaagag aagaggcgca
     2821 tggttaatgg aaaatagtac agaaaaaaaa cgtttaatca caaaaaaaga tcttaataag
     2881 atgtgtcttc gttcatggac aatcatgtgt tcgtggaatt atgaaagaca aatgcatatg
     2941 ggatttatgt atgggatggc gccaatttta aataaagttt ataaaaatga tgaagagaaa
     3001 aaaagagaag cttatactcg tcatatggca ttttttaact gtacgcctca aatgacttca
     3061 tttattatgg gactttgtgc ttctatggaa gaacaaaatg ctcaagcaga agaaggaaac
     3121 tttaatactg aatctatctc catgattaaa accagtttaa tgggtccttt tgctggaatt
     3181 ggtgattcat ttttccaagg tactttaaga attattacat ttgggattgg attgtctttt
     3241 gctaagcaag gtagtatttt aggaccgatt ttagctgttg gcctttttgc tataccttct
     3301 attttagctg ttttttacgg cggtaaatta ggatatgagt ctggcaataa ataccttact
     3361 aaattgtatc aagaaggtct tatggatcga gtaatgcaat ttgcttctat cattggtttg
     3421 gctgtagtag gtggaatggt ggctagcatg gtgccaattg tcaccccttt aaaattcact
     3481 acagggggaa cagaacttgt tttacaagac ttacttgatt ccattattcc tggattatta
     3541 ccacttgtat gtactttggg aatatataaa ttgattaaga aaaatgttag tacaaatatg
     3601 ctcttattag gttgtatttt ggcagggttt gttttaagta taataggtat cttataatga
     3661 ttaaggctct aatttttgat atggacggag ttttaattga tagcgagatt atgtatcaaa
     3721 agtgggtatt taatttttta aaacaagaaa atttaagcta tcctttgtat tcttatcaaa
     3781 aaaagattgg aactacagca agtatttttg atgatataga aaagtacaat gagggatgta
     3841 acagtttaga attgaaggga aaatttacta aatattggaa atcgcaaaag attgattact
     3901 tggatatttt tcctaatata ctaattgaac agcttgaatg gttaaaaaag aaaaaaataa
     3961 aaatggcaat tgcttcttcc agtccaatgg atactataaa tgaagttgtt accacttgta
     4021 aaatagagtc atattttgat tgtttaatta gcggaagaga tttgccagaa agtaaaccta
     4081 atcctactat attccttaaa gcttcagaac agctcagaat tccagttgaa gaatgtctgg
     4141 tgatagaaga ttcttataat ggtataaaag ctggtaaaag agcaaatatg aaggttcttg
     4201 caattaaaga taaacgtttt tctcaagatg taagtctagc agatgatgtt atttacgata
     4261 ttaaatatct gcgacaagaa gtacaggtga aatttggaat ataaataagt tttttgatgt
     4321 atttagagaa tattagtaaa aataaaaatc atgcccaaat ctaatcaaaa ttgattgctt
     4381 tgggtatgat ttcttttaat atcaatctat tactttagga tctgaatcat ttttatgcac
     4441 cgtatctaaa gtgatatttt ctgtgacttc aataacataa ctaattctat cacaacgaac
     4501 tgtggattga gaataatgaa atggttgatt atttttatct ttgccaacct tttctaaaat
     4561 aaatagcgga tctcctacta tacattgcaa taagtcggaa agctcaggtg ttgcagataa
     4621 accgtttatt tccataatag agctcgtagc tttgacatca tagtcatctt ctagaagttt
     4681 atataatgaa gtatattggc tacctttttc aaataaagtt ggaaattttt cttctgatgc
     4741 gaaaaaatta tcaattccca tcggggcacc gtcttcccac attaaacgtt taatattgta
     4801 aactctatca gttacattga tttgtaattt attagctaaa aactcatcag cctttataat
     4861 tttagaatgt aaaacagtat atctaatttt atgtcctcgg gtagaaagag aatcgctaaa
     4921 tccatgaact ccagctaatt ttaaactttc ttttttttga gttacaaagg tccctttccc
     4981 ccttaatcgt tcaactgttc cactttctgc taaaagttta aaagcttttc gaatggtaat
     5041 cttacttact ccataaattt tacataattc ttcttctgta ggtaattgct ctcctggaag
     5101 atattttttt tcacgaatat cgttacgtat agaagcttct acttgtaaat ataatggtct
     5161 attatcttga aaattcatag tacactccct aaaaagttat acttcattat atcacaattt
     5221 attaaaacgt cgattgtaaa attaagctag acaactaaaa agatcatttg tgctacactc
     5281 aaaatagttc cgaccaaaga attaataagg agtgagtaca aatgacttac acccatctta
     5341 caacagacga actagtaatc atagagtctt atttcaatat cgagaaacct gtcgctcttg
     5401 tcgcacaaag tttaaatcgc tctagacaaa ccatttataa tgtttattcg tttctcaaac
     5461 aaggaaaaaa agtgctagat tattaccgtc aatacaaaca aaataaatct cgttgtggac
     5521 gacgttcaac cgttttatct gatgaacaat caacatatgt tcaagaaaag gtagctcaag
     5581 gatggtcacc cgatgtgatt atcggccgtg gcgagtttcc aattgattgt tctgttcgta
     5641 cactctatcg gatgtttaaa aaaggccttt ttgacacaca gtcgttacct atgaagggga
     5701 aacgtaaacc gaataatcat caagaaaagc gtggaaaaca ggcctttcgc agaacgattc
     5761 atgaacggaa cagtctctat ccacacctga ctaaagaatt tggtcatctc gaagtcgaca
     5821 ccattgtggg agttaaacac aagagcgctg ttattacatt ggttgagtgt ttatccaagg
     5881 ttattattac tttgaaaccg acaggcagac gagccgaaga tatcgaaaac aggttaaatg
     5941 attggttcaa atccatgcct aaacacttgt ttaaatccat gacatttgat tgtggaaaag
     6001 aattttccaa ctggcgatct ataagtaact cgaacgatat tgccatcttt ttcgcagatc
     6061 caggaacacc ttcgcaaaga gggctaaatg agcactcaaa cggcttgctt agacgagatg
     6121 gcttacctaa aacaatggac tttagggaca cggatgaaac ctttattcaa tccgttgcct
     6181 cgaaaagaaa ccatattcca agaaaatcat taaattatcg aacaccattg gaagtatttt
     6241 tgagctgcat agataaagac atattgtcta gcttaatttg acaaatcaga tagtataaaa
     6301 tgaatcaaat tgaatatcaa atatatatta tatccatgaa gcccaatctt ctttaataga
     6361 agattgggct atttttatgg caaaggaggg aaaagaaatg tttcaaaata aaggacaacg
     6421 ttatgtcaca aagggagtaa tggacagttt gccggtggaa ttacaagctt tatgttggaa
     6481 tctaatcgat caaaacgttc aaaagcaatt tcatttagac tatcttcaaa tctttgaatt
     6541 ttctacggaa aaaggaaatc aaaagttggt tcatcgtcaa gaagaaccag aagcaagaaa
     6601 agagtatctg atttcaccta agcttcgttt gaaatcagtg tctcaaaaaa tatgggtcat
     6661 tgattcgggt gagtatcaaa cgatgttatt accagaagaa tactgatatc taaaagaaag
     6721 gaacgaaagg acttgttaga aacggctatc gaagtactgg aaaagtgtgc acagctagtc
     6781 actactcctg aagagtgggg ctatgaatcc gttacgatgg aaaaagagga aattgaaatg
     6841 ggtatgcttc ctgaagatgt tactctacca cgtttagtga tgacacatct ttatatttat
     6901 tgcgcaccag aagatggaaa agactatgta gtttatttca ttacagatat taccagccag
     6961 cgagaatttg ttcgtgggtt actggtagaa ggacgattgg tttggagtca gattggagga
     7021 acgaatgaat aagatcgatt atttgagaat gattgcgaaa tgtttagaag atatccttac
     7081 agcgattcat caaattctga agaaaaaagg agaaagaata tgaaatattt tcaactagta
     7141 ttgacactta taacggcaat ggtgattgaa ttgcttgaac aaaaattagc ataggagaaa
     7201 aagagatgat tgaaaaagaa ttagtgacca cacaaacaga agtttggcga gatcctcatc
     7261 atacccatcg ctatgttttc aaacgacaat gggcgatcaa aggaaaagaa gcggaagata
     7321 aattagcggt agtcattact attcgtccaa cagatacaga accatttacg aatgatttga
     7381 gtatgttatt aatcgaaaag aatatccgac aattaggatt tactggtttt attgctgtga
     7441 atcttttttc gtatacaaaa gcgaaaagtc ctcgggtatt tcctcgtgga aatgatgaac
     7501 aatctttgga aatattaaca actgttttca cagaaaagaa aatcaacacc attatttttg
     7561 cttgtggttc gattacagcg acgagtcaag tggcttatga acaagcgaag accatttacg
     7621 atcaattgag tgcgaaacaa aaaaagatga ccaaagtatt gataaatgca gaaggtaagt
     7681 tatcccaccc actaagtatt catgttcgta atgaatggca attggcagat agtcaattat
     7741 tatttcaaga aaaataacat tttattttct tatgttactt ataagtagtt ttacatgctt
     7801 tttgacttta aaaatacatg tgatgataca tgttatatga attatcaatg tacccagtaa
     7861 tatgctattt tcattatgta aaaaataaca gggggtgtgt agatggagat tgatgaaact
     7921 tgtttgagta actcttataa aagtatgaat gacttttttg aggaaatgga actggattca
     7981 gaattgctct atcaatttta tcttgctttt cgagggcaac agatttcttt tccaatgaaa
     8041 atgtacgatc gtgagttagt tagaaaaaga atcgaaaaca tgatagaaca ggaaaaaact
     8101 gtagatatcc gtcaattgac agaagtttat ggtttctctg caagatggat tcaaaaagta
     8161 attaaacaaa aagaaaggag acgagtacat ggctaagctg attgtattag gtggtttaat
     8221 ttacggtgga tattggttat ataaatatat gcttgcgcat ccgaatatag ataaaggaaa
     8281 agtaattaag acagttgggg taatcatagt tggactcata gctctaacta agaaaaataa
     8341 tactaatagc ccaaaaataa tgagaactta tcgctgtaga ggatgtggaa gggaaatttc
     8401 tactagtttt aatccatctg aaagccctca aggaagagat cgttgtcatg ctggctatcg
     8461 acattcttgg gaaaggatta actaaatgaa caccattgag attaattgac tgataaaata
     8521 agtacgcgag ggagaaattg aaagggggaa ataaaatgaa aaaatggttg ttattactaa
     8581 ttggaatggg gattttattc actggttgta catctgagaa tacgaggact aaagagactg
     8641 tagaaactga tacaacaatt gaggctaaaa aaagtaaaac gcaatcaact gaacaaacta
     8701 caacagaaat gaaacaatct gtggaagcaa gtacaagtga aagttcaatc attgaatctt
     8761 cttctgaaga ggttaaaaat actttgtggg atagtgagaa atctactcaa ctagcaattt
     8821 ttgtcccaca atggggaaag acattaggtc aagaatataa aagctacaat cctcagaaca
     8881 atgtaagtct gtatggtacg cctttaccaa gtgcagttat tgatggaaat tggaaaatgg
     8941 caatcaatga agcaccagtt accgtacaat ggtcagaaga tggaactggt catgcagatt
     9001 tcaatttagt agcagtttat tctgatgtag aaactggaga atatttaggg cagcatgtat
     9061 atttctttgg ctttcaaaat ggacaaccta aagtttattt aacgcaacaa aatcaaggca
     9121 atgataaaaa ctacatgtac ttcaatgaaa ctcagaacca acagttaaaa caaggtttca
     9181 ttgatattgt gaatggacaa actcctcaaa ctccagtagt agaagaatct actcaacaag
     9241 caacaactac aacgacacaa tctacaagta gctcagcaat ttcaagttgg gaagctgcta
     9301 aagaaactgt actaaataat aaagctttat ggttgtatga ttctcctgat aaaaatgcat
     9361 caaatactaa tttaattagc acaaatgaat atatgaaaga tctaatgcaa gatgaaaatg
     9421 gaagctacta ttcaattaat attgtaacgg atgataatgg aaatacaaca atggctggtg
     9481 gaaaacaatt ccgtgtttat acagatggaa gaatttctca acgtattgga atgactaaca
     9541 attttcaaat gatt
//
LOCUS       DADVXU010000098         9438 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.102, whole genome shotgun sequence.
ACCESSION   DADVXU010000098 DADVXU010000000
VERSION     DADVXU010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.102"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(130..777)
                     /locus_tag="KB953_002355"
     CDS             complement(130..777)
                     /locus_tag="KB953_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HBB6867293.1"
                     /translation="MEQTIKPYQYFFIKREVDQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILEHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(1056..1559)
                     /locus_tag="KB953_002356"
     CDS             complement(1056..1559)
                     /locus_tag="KB953_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBB6867294.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(1682..2446)
                     /locus_tag="KB953_002357"
     CDS             complement(1682..2446)
                     /locus_tag="KB953_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HBB6867295.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     NAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLADVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            2553..2828
                     /locus_tag="KB953_002358"
     CDS             2553..2828
                     /locus_tag="KB953_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HBB6867296.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            3505..4455
                     /gene="yidC"
                     /locus_tag="KB953_002359"
     CDS             3505..4455
                     /gene="yidC"
                     /locus_tag="KB953_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBB6867297.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(4634..5608)
                     /gene="lacD"
                     /locus_tag="KB953_002360"
     CDS             complement(4634..5608)
                     /gene="lacD"
                     /locus_tag="KB953_002360"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HBB6867298.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDTKIPGR
                     LPDLLNIWSVKRLKEAGADACKFLLYYDVDEPAEINVQKQIVIERIGSECEAEGIPFF
                     LEILSYDATIGDPSSLAYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QETCYSQAEASAYFYEQSKVTEQPFIFLSAGVNATLFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATPVYVDR"
     gene            complement(5605..6063)
                     /locus_tag="KB953_002361"
     CDS             complement(5605..6063)
                     /locus_tag="KB953_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBB6867299.1"
                     /translation="MTIIHEENIFLQQPLDSQEEAFKFLAKQTFCLGIALDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSMIIATLNKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRKAKSKTEIATILTKEIGEDL"
     gene            complement(6060..7523)
                     /locus_tag="KB953_002362"
     CDS             complement(6060..7523)
                     /locus_tag="KB953_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBB6867300.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGIKIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFAGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAMSGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSFDPDHAQYNATAAMVKAVGDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLTILPKQLDGLKAIFLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEHSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLAQGN
                     TTFMAGVSAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKMDNKAANRTVIEKGRAGDLS"
     gene            complement(7505..8455)
                     /gene="pfkB"
                     /locus_tag="KB953_002363"
     CDS             complement(7505..8455)
                     /gene="pfkB"
                     /locus_tag="KB953_002363"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBB6867301.1"
                     /translation="MSIYTCTMNLAIDLFIETEEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPKIPQTAVHNFLNQIRALQAEDYLCVSGSLPRGLSPKVLIEISRICQEKGI
                     HLIIDSSDQEAMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVELGAENV
                     LLSLGEKGAILFTKDRVFRGNSPKGKVVNTACAGDTMLGAFLAGYMNRRPLDETLRKS
                     IAAGSSTAFRKGLTDFLDVEELSKQIKIKEERFETWENIN"
     gene            8598..9347
                     /locus_tag="KB953_002364"
     CDS             8598..9347
                     /locus_tag="KB953_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBB6867302.1"
                     /translation="MDHKLSETEQYLWNFIEHHILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGETKELVTAAKNCYDAEIGSILVTANHSSTLKDYCEINLVGFKSEGS
                     YFPDYEVRSRLPLQIIARILLDAYVLRMGEN"
BASE COUNT         2805 a         1998 c         1639 g         2996 t
ORIGIN      
        1 aaagaaaggg gctatcccca gactttagta ggcgtcatta gctatctttc gttagcattt
       61 aaaggcgaac accatcacct attcacttcc attaaaagat agcatatttt tctgtaaaaa
      121 acaataaaat tatcgttctt tctttacacg ttcagcaaat tcatagaaag gagccaattt
      181 cgttaactgt tgattacatt ggtcactatc atgacaaata taattcccct ttttcgtata
      241 cgtgccatca gaacctgctt tcgttgtaga aagaaaaagc gacacattcg atgttttctg
      301 acagattgca cagattccct tatttatcgt tggtgataaa gtaccgctaa tcccttttaa
      361 tttttcatct tcgtaataga ccatgaattt tttctgcgta cctggatcat tccagccgat
      421 gtacgtatgt tcttgcaagt cgatttcatt ccaagctggt tgtttgagtt ttttcacttt
      481 tcggaataat ttctcgattt gtggtttgct aggctgcata aacggctgga cgatttgctt
      541 cagctcctcc aatgctcttt ctgcttgcgc ttttgtcagt tttacgtcta aaactgtcgt
      601 taaaaatgta tcgatttctg gcagttcgtg ttctaatatg tctcgaatct tttcagctgc
      661 tagtgcctga actgtttgta ccgtttttgg atcattgaca gatgagtacg catttaataa
      721 ctgatctact tctcttttta taaaaaaata ttggtatggt tttattgttt gttccatgat
      781 tttaaagtcc tttcttctaa ccaaagtgca tactttgtag aaaaaagtga tcttctgcaa
      841 agtatgtgtt acctgaagat cccggactct tttaaataag atacgttttg catcttatca
      901 tcaccccctt taatgttcat tcttctatgt acatgattca ttcgataaat aaaagtaaac
      961 cgtatcttga acagatagtc aactgcaaac agaaaaaacc gagaaaatca gccagatttt
     1021 ctcggaatat agtctattat tattggtttt acctattaaa tcgaggattc tttcccaaag
     1081 taacgttgcc atctcttacg catacttgca gacatgggct gagccgcttc tttgactgcg
     1141 cagtatttgt ccacgaatgt cacaatatat ccttctttat acttaggcgg tgcgatcgtt
     1201 gcaccccaca tgtgcttcaa aatgatatct cgctcaagat ccgacaaatc ggtgattttt
     1261 tctgcattct ttacagcaat tcttggatgg atatacgcat gtgtcccctc gtcaaatttc
     1321 gtcgtacgcc aatcatagta gaacagatca tgcaaaagtc cagcacgcgc tgttgcttta
     1381 gcattgccac cccatttttt tgcgagcaga taactatagt aagaaacact gatggaatgc
     1441 tccaaacgag tggaatgaac atgctgtgtg taattcgcta attttttcac tgcttctgtt
     1501 tctaaaagat cttgtacata agacatatat tcttcgtctt cacgccattt ttctgtcata
     1561 attatcgatc tgccaccttt ttgaatttca ttttcttctg aaattgatct aagtatacca
     1621 ccagtgttcg gattattcta gtcgctctag ataaaatagc taaagcttaa tagttcctta
     1681 atcataaaga aaaatgataa agaacgatcc ctgctgcgac tccgacattc aatgattccg
     1741 catttccttt cattgggata tatacgttct gatcagtcaa agacaagatt tcctgactta
     1801 ctccctgacc ttcgtttccc atgattatag cgaaatcctc tgttttttct actttgtcta
     1861 atgcatgtgc ttcttcattt aattctgtac cgtacaccgt aaaccctttg cctttcatta
     1921 cagggattac atctgccaaa tctttttgta acacaggcaa atgataattg cttccctgca
     1981 tgcttcgcag cacctttgta ctataaatat ctgccgttcc tttacctaat aaaacaccag
     2041 ccataccaaa cgcatctgcc gtacggatca tcgttcccac atttccagga tcctgtacat
     2101 tatctagtaa aagccagcct cctgaaaaat ctggcgtatc ttcagatgtt aacgtcatca
     2161 ctgctaagat cccttgcggt gtaggtagat ctgacatagc ggtcatgacc tcatctgaaa
     2221 ctagaaaagt agaaatctct ttgtttacag ccagccagtc tccccattct ttcatacctc
     2281 tctgtgtcac aaaaagttct tttatttctg cattagacga cgcagcttct tcgatcagat
     2341 ggaacccttc caaaagatat tctgcctttt cttctctgta tttcttttta tgtagttttt
     2401 ttaattcttt gattcttgga ttttttgctg attggatctc tttcacattc ctcacctcaa
     2461 ctgattatat catggaatag gtgcttgagc ttgtttcatc ctaaaaaaat cggtatcatt
     2521 gatttataaa acaggaaaga aaggtgacga acatgcgcaa agttaaaatg aatgtccaag
     2581 gacgtgtcca gggcgtggga tttcgctata tgaccaagat ggttgctgat cagttaggcg
     2641 tgaccggaac ggtaaaaaac gaagacgacg gctcagtcac aatcgaagca atcggaaatg
     2701 acgacatcat ccaaaaattt atcgaagaag tcaagaagtc tccgagccca agtggacgag
     2761 tgcagtatgt ggatgtccaa gaagatccga tgattgagga acgaaagaaa tttgatgtag
     2821 tgggataagg ttgtgaaaag gtcgcttagt tttgagtatt aaggaaaatt ttgtgaaaat
     2881 ggctttccac atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg
     2941 tcgataaggt tgtttttgaa gcgctttgcc tcgagtatta aggagaaaca ttttcaaaat
     3001 agcttctcat attttgaaat gtaatgactt aataccgaga ggcagctgat aaaacaccgt
     3061 caataaggtt gtgaaaaagc tggtctgcat caagcattaa ggactggttg tgataaatag
     3121 ctctccatat ttttcaacag gctggactta atgccgtaga tgcaagcttt tgaacaccgt
     3181 cgataaggtt gtttttgagg cgctttgcct caagtattaa agacaggtag taaaaaagct
     3241 gatctataac ttgcaatgaa tttgcttaac ccatattttc cagacagaac aaaaaagttt
     3301 ttagtttttt aaataagaaa gaaacacttg gaatcactct tttctttcta agtatttctt
     3361 tcttgatttt ttgtcttttt tcaaaatttt tcgctacgaa aaagaacgaa tatttatatg
     3421 aaatttctat gaagttgatt gaattccatg cgctttttta gtatagttaa gtttgtgtaa
     3481 aaattataga agaggaagta ttctatgaat aaatggaaaa actggttatt gggatccgga
     3541 cttgtcagta tcctgctctt tttatctggc tgtgtgcgga tggattcaaa cggaaaccct
     3601 gacacttcag gaatcgttta tcgagtactc gtccatccta tgggccaagc gattacatat
     3661 ctagttgaga atttcaattg gtcttacggc tgggcagtta ttgcaatgac tgtgatcgtt
     3721 cgtatcatca tcttgccact aggtatcagt caatcgaaaa aaacaatgat ccaatctgaa
     3781 aaaatgcaag cgttaaaacc acaagttgaa gcagcacagc aaaagctaaa agctgcaaca
     3841 acacgtgaag aacaaatggc agcacaagca gaaatgcagc aagtatatcg cgaaaatgga
     3901 ttgagtatga ccggcggaat cggctgtctg cctctattga tccaaatgcc gatcttctct
     3961 gccttgtatt ttacggcacg ctacacagaa ggtatccggg aatcatcttt ttacggcatc
     4021 gatctaggct caccaagtat ggtgcttgtg gccatcgcag gggtcgctta tctgcttcaa
     4081 ggatatattt caacaatcgg tattcctgaa gaacagaaaa agacgatgcg tacgatgctg
     4141 atcgtctctc ctgctatgat cgtcttcatg tcaatcagtg cgccagccgg tgttacactt
     4201 tactgggtag tcggtggtgt gttcagctgt ctgcaaacat tcatcacaaa cgttatgatg
     4261 aaaccacgtt tgaaagcaca agtggcagaa gagttgaaac aaaacccgcc aaaacaagtc
     4321 gtcactccaa gaaaagacgt gacagaatca gcaaagccag ctaacaacaa acaagcaaca
     4381 aaacaaatca aacaaccaaa aaacagtaca aacaacaaag gccgcaacgc aggaaagcag
     4441 cagaagcgga agtagatagt aaataagagt gtaataaaaa ttcattaaca gttttagcaa
     4501 ttaaaaatat cgaccaagat ttttgaaaaa gaaatctggt cgatattttt atttaagtga
     4561 atgtttaatt tgaatcatcg attgatcgct atctaattgc agcagcatct ttttatgaag
     4621 aaacaacttt ttcttatcta tcaacgtaga caggtgttgc tgtttcttct aatagtgtac
     4681 ttaattcttt aatattttgt tttccttctt ctaacatcca actactcgct ttttcttcgc
     4741 cttcggtaac aaaaggtgcc actcccttcg cccatgttgc tcgcccacat aagacaccat
     4801 tgaatgtcga acctgattgt tttgcaaaac gtaatgtatc ttgaaatagc gtagcattga
     4861 ctcccgcact taaaaaaata aacggttgtt cagtcacttt cgattgttca tagaaatacg
     4921 cggatgcctc tgcttgacta taacaagtct cttgcccata tccttccaca aatctcatat
     4981 tcactggcac ttctactttc aatacatcca cttgataacg ttcttttgaa aattctcgca
     5041 tggcttcaat aactttatgt ggttttactt ttgcataggc aagagaagac ggatcgccaa
     5101 tagttgcatc ataagaaaga atctctaaaa agaaaggtat gccttctgct tcgcattctg
     5161 aaccaatccg ttctataact atttgttttt gcacattaat ctctgctggt tcatcaacgt
     5221 catagtacaa taaaaattta caagcatccg caccagcttc ttttaacctt ttcaccgacc
     5281 aaatattcaa taaatcaggt aatcttcccg gtattttcgt atcatagccg gttttttcat
     5341 aagctagcag taagccacat tctttctctc gaagcgctgc tgcaggcagg ccgtattctg
     5401 gatcaagtaa aatagctgag gcatagggag tcaggtattg agaaaccatc tgtttaaatt
     5461 ggatgatgtc ttctgatgta gcttctttat attggttgat cattttcttc aaagcaccac
     5521 gctggtcgat tgctaatgcg ctaataattc cagcttcatc cgtcaatcgc ttcaaacgtt
     5581 cctgtttttc tatactcatc ttcttcatag atcttcacct atttctttag ttaatattgt
     5641 ggctatttct gtttttgatt ttgcttttct caactgttct gtaacctctt ttcgcatcaa
     5701 tgatcgtgca acgagagaca ataatttcaa atgagtcgtt cctgcttctg aatctggaat
     5761 aaataacgca aaaataaaat caactgactg tctatctaaa ctgttccatt caattccttt
     5821 gttcagtgtg gcgataatca tactaggttc gttgattgtt tgagacttcg catgagggat
     5881 agcaaaacct gacattattc ctgtagtccc ttctttttct ctttctagaa gtttttgata
     5941 tacttctttt tcgtcaagag cgatccctaa acagaatgtt tgctttgcta aaaacttaaa
     6001 tgcttcttct tgactatcca atggctgttg taaaaaaatg ttctcttcat gaatgattgt
     6061 catgataagt ctcctgctct tcctttttcg ataactgtac gatttgctgc tttattatcc
     6121 atcttcttgt ctttatcagt tactcttttc ttttggatta atcctagtat cactccacca
     6181 acgatcgaac caataagaat cgctgtaacc cattggattt ttcccgtcac tacaggtaac
     6241 actaaaaaac caccatgtgg tgctggaact tggacgccaa acaaataagt taagacagca
     6301 gcaattgatg agcctaacat catcgtgggt aacactcgga tcgggtcttt cgcagcaaac
     6361 ggaatagctc cttccgtaat atgagttgat cctaaaataa aattaaccaa acctgcatta
     6421 cgatcattgg tatcaaaata tttaccaaac aataaagtag caaaaccagt aatcaacggt
     6481 ggtgcaatac aagctgctga cacaccagcc ataaaagtcg tgtttccttg tgccaaaaga
     6541 gctgttcctg ttacatatgc cgccttatta accggtcctc ccatatcaaa cgcacacata
     6601 cagccaacaa tcacgccaag aacaagtgga ttcgaatgtt caaatccagc taaaaagctc
     6661 atcattcctt catttatagc cttcattgga cctgccagca aagacatcac gatgccaata
     6721 aatgctacac caataactgg atataagaag attgccttca agccatctag ttgtttaggt
     6781 aaaattgtca gtagcttttg caaaaataag ataaaataac ctgctaagaa cccagctaaa
     6841 atacctccta aaaatccagt accgccattc atggctacga ttccaccaac aaatccaacg
     6901 actaaccctg gacgtttcgc gatcccttct gcaatataag ctgctaaaac tggaaccatc
     6961 attcccattg ctgcatcgcc aactgctttt accattgccg ctgtagcatt gtattgtgca
     7021 tgatctggat caaaagaata gattccccaa acggcaaatg aaatagcaat caatactccg
     7081 ccagctacca cgaaaggcaa catatgagag acaccgttca ttaaattttt atagatgcta
     7141 cgaccaaaat tagcaccact catcgcttct ggttctaaag tatcagcagc ttcattatct
     7201 gctaagacag taccttttcc tgccaatgct tcgtcaatca gccgttgtgc atccttgatt
     7261 cctgtactga cagaaacatc aatgactcgt tttcccgcaa atctttctga atggacatct
     7321 ttatctgcgg caataatgac agcatctgcc tgtttgattt cttcagccgt taattcattt
     7381 tcgatcccaa tttgtccatg ggtctccact ttgatcttga tgcctttttt cctagctgct
     7441 tgttctaatg cctcttgcgc catgtaagta tgcgcaattc ctgtcggaca accggttgct
     7501 gcaattagtt gatattttcc catgtttcaa acctctcttc cttgattttg atttgtttac
     7561 ttaattcctc cacatccaaa aaatcagtca gtcctttacg aaaagcggtt gagctgcctg
     7621 ctgctatgct tttacgtaac gtttcatcaa gcggtcgtcg attcatatat cctgctaaaa
     7681 aagcgcctaa catggtatca cctgcacatg ctgtattgac aacttttcct tttggcgagt
     7741 ttcctcgaaa caccctgtct ttcgtaaaaa gaatcgctcc tttctcaccg agggataaca
     7801 aaacattttc tgcacctaac tctactagtc gttgtccata aacaaagtaa tcttcatctg
     7861 taaccatttt tcgtccgaac caagaagaaa gttcttcctc gttcggtttc agcaaaaacg
     7921 gtcgataggg taaacaatcc atggcttctt gatcactact atcaataatc aaatgaatcc
     7981 ccttttcctg acaaatccga ctgatttcta ttaaaacctt tggagacagc cctcttggta
     8041 aactcccaga aacacaaaga taatcttctg cttgtaatgc tcttatttga tttaaaaaat
     8101 tatggacagc tgtctgcggt attttaggtc cttgattcac taatttgtat tcttctcccg
     8161 tttgattgac ttgtgtaaat acattgatac gcgtcatacc aggcacttca ataaactctg
     8221 tttggatttg tttctgctgt aaatagtctt ctatatactt tcctgtaaac ccagcagaaa
     8281 aacctaatgc cgtacttggg aaatctagca tttttaatac tagtgacaca ttcacccctt
     8341 ttccgtttgc ctgaatgtca tcctcttttg tgcggtttac cataaaagga tgcatctcct
     8401 ctgtttcaat aaatagatca attgccaaat tcatcgtgca tgtgtagata gacataacca
     8461 acgacctcct tgactctatt atgaaatacg cgaaagagaa aatgttttct taatcgtccc
     8521 aacacaaaga aaacctttac atttataaac tcgtcgtgct attttttaac taacttttgg
     8581 tgaaagaagg aaagaatatg gatcacaaat taagtgaaac agaacagtac ctctggaact
     8641 ttatcgaaca ccacatccta gagatcccta actactccat cgtgaaacta agtgaacaag
     8701 caaatgtctc aacagccacc attgtgcgga caatgaaaaa gaaaggctat gaaggattta
     8761 cttcgtttaa gcaccatcta aaagaaggag aaaacaagaa catcaatttt tctttcttag
     8821 ataaagtaga caaaggtatc cgtcgagcca ttctaaaaaa tgaacaagaa gtggtacgta
     8881 cgatcaatat gttggaaatc ggaaatattg aggatgcaat acaaagaatc aaatttgctg
     8941 accgggtttt tgtctttgca cgaggatttt cagaaatgat tggtcaagag atgctcgtaa
     9001 aacttcaatt aacagggaaa aattgtcaaa tgcatactga tccagaaatc atcaaaagca
     9061 tcagtcggaa actaactaag aaagacagcg ttatttttgt ttctttgaac ggcgagacaa
     9121 aagaactcgt caccgccgca aagaattgct atgatgcaga gattggttcg attctagtta
     9181 cagccaatca ttcctctaca ctgaaagact attgcgagat caatctcgta ggtttcaaat
     9241 cagaaggttc ttatttccct gattatgaag tacggtcacg tttgcctctc cagattatag
     9301 cgagaatcct gcttgacgcc tatgtattgc gtatggggga gaattgaaaa gcagtttatt
     9361 atggttggtt acagtttaaa acatcacatt ttttattctt tatattgtat ggactcacgg
     9421 aaacttttta tgtataaa
//
LOCUS       DADVXU010000099         9408 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.103, whole genome shotgun sequence.
ACCESSION   DADVXU010000099 DADVXU010000000
VERSION     DADVXU010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.103"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            112..306
                     /locus_tag="KB953_002365"
     CDS             112..306
                     /locus_tag="KB953_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867303.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            575..874
                     /locus_tag="KB953_002366"
     CDS             575..874
                     /locus_tag="KB953_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867304.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(971..1405)
                     /locus_tag="KB953_002367"
     CDS             complement(971..1405)
                     /locus_tag="KB953_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB6867305.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1637..2347)
                     /locus_tag="KB953_002368"
     CDS             complement(1637..2347)
                     /locus_tag="KB953_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBB6867306.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2337..3197)
                     /locus_tag="KB953_002369"
     CDS             complement(2337..3197)
                     /locus_tag="KB953_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HBB6867307.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3202..3840)
                     /locus_tag="KB953_002370"
     CDS             complement(3202..3840)
                     /locus_tag="KB953_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HBB6867308.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3855..4154)
                     /locus_tag="KB953_002371"
     CDS             complement(3855..4154)
                     /locus_tag="KB953_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBB6867309.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4197..5681)
                     /locus_tag="KB953_002372"
     CDS             complement(4197..5681)
                     /locus_tag="KB953_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBB6867310.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5701..6162)
                     /locus_tag="KB953_002373"
     CDS             complement(5701..6162)
                     /locus_tag="KB953_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBB6867311.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6180..7247)
                     /gene="ulaG"
                     /locus_tag="KB953_002374"
     CDS             complement(6180..7247)
                     /gene="ulaG"
                     /locus_tag="KB953_002374"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HBB6867312.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7465..8235
                     /locus_tag="KB953_002375"
     CDS             7465..8235
                     /locus_tag="KB953_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBB6867313.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8508..9335)
                     /locus_tag="KB953_002376"
     CDS             complement(8508..9335)
                     /locus_tag="KB953_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6867314.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2925 a         1932 c         1476 g         3075 t
ORIGIN      
        1 aaaactttca aattgattct ttttatttat cataaagata tagatacata atcattttca
       61 cttacatata tttgaatatt aactaaaaac agcaagaaag aagaggatca aatgtattat
      121 gtagaagtaa aaacaaaagg cgtcaaaaac aaacaatatg tgaaaggtat cagtaacgaa
      181 tatcctttac ttggttcatg gaaagaagct gctccttttt ccaaaccatg tgccataaaa
      241 ataaagaacg agctagaaaa agaactgact tgtggtaaag ctgttgtaga tatcatcgaa
      301 aagtaaatcc aatgtataga aaggaagatc acacatgaaa agcgacgtac aaattgctaa
      361 gattatagaa ttagaagaag aagacgcagt gttgacaatt gaaaaaaaat aaatgtttgt
      421 ataccatatg attatttcaa tatgtatcct ggaattggag atctcgtaga gctatatcaa
      481 gatgaatcaa atatacttgt cattcatcat gaaatataga gacttataaa attgtttatt
      541 tttataatgt atcagtatag aaaggaaata aaacatggat tatactgttg aatttgacag
      601 tgaactactt aagcaatttc cacaagaaga acatttcatg tataaagaag caatttccca
      661 caaagtgaca gagtatttgg agcataattt ttttcgtata aagccagtgc gtactcgttc
      721 ggcaaaagga atatacgaga tgaaagtcaa aattaataaa aaaaattatc gcatagcatt
      781 tgctttaaaa aaaaatcatg tgttcatttt ttatattagt tcgaatttgc aaaaaattgt
      841 atttgataaa gaggtgagta aaaaaataaa atagctgagc attggaggat agattgagtg
      901 atactaagtc tatcctcttg tttttctaaa gaactaagct agaatttttt cttttgtacg
      961 ttattatatg ttatttgtca ttaccaaaaa gcaccttttt tcttctagta tctatgacat
     1021 aagaaatacc acccatttcc tttacttgaa caaatcctac tgattcataa aatttttttg
     1081 ttttctcagt atcatctgtc aataattcga tttggactgg tccataacta gtgatcttct
     1141 ccaaagcttc attcaaaagc actttgccta ttccttggcg ctgatacctc ggatgaatga
     1201 ggatatcttg aataaatgcg atcgtcgcaa aatcagtgat ccaacgaacg attccgataa
     1261 tcttcccttc cccattacgc tttatcagag attcagattg ttcaaatgct ctagctaacc
     1321 ttgcagtgtc ttttgtgtaa ttcgtccaac caacgctctt atataattca gaaatctcat
     1381 cgatcataag ggaatcaata ttcatttttt tcttcctttc ttatacaatt ctccctctaa
     1441 taatacagtt ctttgactat ttaatctatc acatataact ctcctgtaaa gattctctcg
     1501 attccttaaa attcctcttt ccttttagaa tgcatacttt ttgataaata aaaaggttgt
     1561 gataaatttt cttcacaacc tctgtttctc tctatcaagt gttatctttt aattttctac
     1621 ccttacccta gcatctctat tgcccgtagt atgcatactt tccatgtttg cgaaaataat
     1681 gtttatctag taaatattgc gggatttcac atatattggg gttgatcagc tcgttttctt
     1741 tggccatcag acaaatttca tctaagatca aactgttttc tactgctttc ataggtgaat
     1801 ccccccatgt gaacggtcca tgtccataga caagtactcc agggacttcg tttggatcta
     1861 attttctttc cttaaacgtc tcgacaatca cattccctgt atgaacttcg taagcttctc
     1921 ttacttcttc tttcgttaat tggcgtgtac aaggaacctt accataaaaa gcatcagcat
     1981 gagtcgtgcc ataagctggc aaatcgcgtc ctgcttgcgc ccacatgacg gagtgcgtgg
     2041 aatgagtatg agtaatagca gttatatctt caaaagtttg atacaaaaca acatgtgtgg
     2101 ggagatctga agatggtttt aagctatctt cttctattat atttcccgac aaatctgtta
     2161 ccaccatttg atctgcttgc atgcattcat atctgacacc gcttggctta atgacgatga
     2221 ctcctagctc acggtccact tcactcacat tgccccaagt caatttgact aatccgtatc
     2281 gcggcaatgc cagatttgct tcaaggacac gttgcttcat tgctgcaatt ttttcttcac
     2341 aagacatcat agccagcctc cttcaattta gaacttaagt agtcttttgc ctgctggatt
     2401 tctcttcgaa agtcttggct tttttcactc cacatttcaa tcagaaatgt tccatcataa
     2461 tctagacgtt tcaaatgtct taataatcca agaaaatcaa cacaaccgct accaaaagga
     2521 acatcacgaa actggcctga atattctttt gttacagggt aagtatcttt taagtggata
     2581 gcagtgatat aatcgatccc tttctcaagt tctcgagccg gattattttc cggccatgcg
     2641 gacaaattgc ctaggtctgg atatacttgt agaaaaggtg aatgaatctg ttctttgatt
     2701 tctaaaaatt ttgaaatgga gttcataaat ggatcatcca tgatttccac ggaaagaatg
     2761 atgccatatt tagaagcttc ctttacgctt tcttttattc cttcgataaa gtcttctctg
     2821 gaattcattg atttctcttc ataataaaca tcgtatccag ctatttggat tactttgacg
     2881 cttagattat gcgccaaatg gattgctttt ttcattagtt gtttcgctgc ttttttcttt
     2941 tctggatcag cagaaccaaa cggaaatcgc cgatgcgcac ttaaacaaat cgaatggata
     3001 cgtacaccgg agctgaacat ctcttctctc agctcagcta tcttttcttc agaccaatct
     3061 aatcgtgcta accgctcatc cgtctcatca atcgacatct cgataaaatc gaagtccatt
     3121 tcttttacaa gggaaaaacg ttctttccat gaaatatctt tgggtaacgc tttttcataa
     3181 atcccaattt gagccatttc cttaccccca gatacgaatg atctcatctt tgaagttttg
     3241 tgcggccttc atcggatcat ttgctgctgt aataccacga ccagtgataa aggtatacac
     3301 atcgatccct tcaaataagt gtaatgtgtg tggatttaat ccaccagtta cagaaacatt
     3361 aaagcctagt tcaatcagtt ttttgatctt cgataaatct ttttcacccc aagtttcacc
     3421 agataacagc gcatcacggc tttgatggta gattacctgc cggataccga tgttatgcca
     3481 atccatggct tgttcaaaag tccaattgcc gtagagttct acttgcaatt cacctatttc
     3541 tttttgtgct gcctccatag ttggaatcgt tgcacaacaa atgactgtca tccagtctgc
     3601 tcctgctttt gcaacattac tcgccactgt tcctccagcg tctgcacatt tcgtatcagc
     3661 gactagtttt ttatctggga acatactgcg aatacagcga atcgcttttt gtccttcctg
     3721 taagcaaaga attgtgccca cttcaataat atctactaca tcaccaactt tcataacatc
     3781 tcctaatgca tgttctaagt cattgtgatc taacgcaatt tgcaaattcg gcctgctcat
     3841 cttagttcct tcctttactt gtttttttgg taccactttg tttcagcaaa tttttcactg
     3901 atttctttga ctgacatcac atttttgata ccgataactg taactccttt tttctctgcc
     3961 tgagtaaaca tattcaaaaa attgactggc gtaaaaacta catcgtattg gccagctgaa
     4021 ctttttccct ctgaaatggc acaatgatgg attttggtaa ttggtacgtt tagttctttc
     4081 attgcttttt ccacacttct catcatcatc aagcttgttc ctgatccatt tgcacacgat
     4141 actaaaattc tcatcttaat ccctccatta tgagtgttgt ctaatcagat tttcttttat
     4201 actctctgct tttctgcata tgtgtcatag tcttctacaa tcaaaaaata accatcagga
     4261 tttttccgat attgtaactg cggaatagcc aataaaccaa tgacaactac ggcaattcca
     4321 gcataaccta ggactttcat gattactgta aacaatggcc aagctgttgc ccagtcgaac
     4381 atcccgatat aaccaccgta tgttgacatc ccaatccatg tagcgaataa tgctgaaccg
     4441 cctacttgta tgattcctga aagaaacggg aacaggcaag cggctttgaa tccacctcgg
     4501 ttattggcat aaactgctat aacggcatta tcaaagaaca acggaatgaa accggctatg
     4561 acgatcgttg gtgatttcaa taaaatcagt aaaccaatca tcaaaaactg tcccaaagca
     4621 ccaaacaaga acccaatcgt taccgcattt ggcgaaccaa agccaaaagt agcagctacg
     4681 tcaattcctg gaacagctcc tggtaaaatc gtgtttgaga ttccttggaa agattgagtt
     4741 agttcatcga cgaatgtccg aacacctaat tgcaagatcg ccagatatac cgcaaaattt
     4801 aaagaagtcg tcataatgta gaataagaaa ctctgccctt cttgcatgaa ttgcgcttga
     4861 atcaaataat ctttgcctag tacaatcaga atgatcccaa agaaaaatag catgagtaac
     4921 gaagtagcaa ccatattctc attgaaaatc gacaagaatc ctggtaattt gatatcttca
     4981 atttttttgt cttgcttgat tgatctattt ttcttttcat cccgcttttt catccactca
     5041 gctaattttg caaatataaa gattccgaac atttgctgat gtgccactgc aaatccggca
     5101 ccttctgtca attcctgcgt gattcctact gttaagttag aagctactgt ccaataacaa
     5161 cctaaaatca gtcccataaa aatcaatgct tgtatcctac ctagatcagg aaagcaaaac
     5221 agtaaaatcc aaaaagcggt agctgcttgt tgaacctgaa cgtttccggt agtgaaaacg
     5281 gaacgcagtt tcgtatattt ctgaaaacgg actaataaga tatttaaaac aaatgcaatc
     5341 agtaataaaa tcatcacatc tccaaaggta cggccaaatg tctccattaa tccagcatcc
     5401 acagcatttt gtccaaaata aggatctgta accatggcat ccaaattgaa ccgttccttt
     5461 agtccaacca agattggccg aaaattattg accaaaccac cagagccaac agtcaaaata
     5521 aagtaaccga ccgttgcctt taagaatcca gcgatgcatt catgaagcgg gcgtttcagc
     5581 aaaatatagc ctaataacac gataaaacca atcaggtatg ctggctgcgt caagatattt
     5641 gttgcaaaat acgtccaaat gcttaacaaa aattccccca tcttttttct cctctatcaa
     5701 ttaattatat tctgccatca caacttggta atcatcgatg gagttcgtag agatgagacg
     5761 ctcaaccaat ccatctgtca ttaataattc ggataatttt tggatatttt ctagatgttc
     5821 ttctggatct tttgctgcga gtgtaaaaaa caaactagcc gttttctcct cattctcccc
     5881 ttcactttca aaacggattg gctgtttcaa cttggtaaat gaaatagctg ttccaaaaac
     5941 accatcactt ttttctgaag aatgcggcat tgctacttga ggtgcgatca caatataagg
     6001 accgtatttt tctacacaat ggatgatctc ctccacatac gtttcatcaa taatttcctt
     6061 atcgatcaat acaagacagc tttcggcgat agcttcacgc caatccgtga tttctttatc
     6121 tgggtaatga atcaattgat ggtcaaaaaa gtatttcagc aagaaactcc ccttctttct
     6181 tataaaaacg acggaaacgg tgtatcattt tcaatcgaga agacatcttc aaatcctcga
     6241 taaaaattaa actcaagatc atctttgttt gtcggataga tgaatttccc gcctacttgc
     6301 cagatataag gtttaaatcc atacttcagg cgatcctttt tgaatttcca aatttcagtg
     6361 atctcttttg gatcagccat aaaattagac caaatatcgt agtggacagg aatcactact
     6421 tttgcattca atgactccgc catgcgcagc atatcaacag aagtaacttt gtctgttatc
     6481 cctcgtgggt tttcaccata ggcccctaaa cagacatcga ttttgtgttc attaccatgt
     6541 ttggcaaata aattggagta atgtgaatct cctgcatgat aaagattccc tcctgatgtt
     6601 tcgaataaat agtttactgc aatttgatcc atctcctgag gcatttttcc ttttaatacc
     6661 tcttctggat cttcacaagt gaccaacgct gtccgatcaa acgcttctaa agctactaca
     6721 ttgatgtctt tgattttgac tgcatcacct ggctttactg taatcgtttt ttcttcagga
     6781 attccccatt tttgccacgt gcgaaccact tcttttggcc caatgaatgt tgcttcagga
     6841 caattttgat gaactgctgc ggctgtatgg atatctagat gatcggaatg gatatgtgta
     6901 acgattaaag cgtctacatt ttttatcgca aaaggatcga ttacgaaagg ctgagtacgt
     6961 aaattaggct gcatattttg aacgccactc atacgcatca tctgatggcc ttttttcatc
     7021 atgccattcc catgactacg ttttcccgtc ccacaccata gatcacataa aatattcgtt
     7081 ccttcatgac ttttcagcca aattcccgta cagcccagcc accacatagc aaaagttcca
     7141 ggatccacta cttctgtttc gatttcttca ttcaagtatg tgccccactc aggaaatgtt
     7201 tctactaacc acttttcttt tgttacttca ttgacagttt tactcatttg cttgctcccc
     7261 ccatatcgtt gttccaactt acaagtaaat attaatgtgt atcagcatcc atgaaaaccc
     7321 ttttatatga ccgtttgttt tttatatgat tttctttcag taatcaatga ttgtttcagt
     7381 ataataaaga cagatatgaa tgtttaaatg aacaactagt caaataaatc catggatcaa
     7441 gatgtccctc aggaggcaaa agtcatgaaa aactcacttg tcaatattga aaaacgtcaa
     7501 aaagaaattt tggaactgct tcagcagact gaacaactat caactttcga attagctgaa
     7561 tcccttgatg tatctattag tacgatacga agagatttaa atttattaga agaaaaaaac
     7621 gagatcatac gcaattacgg ttattgttct tataattaca aaaaccagac tgattttgat
     7681 caatctggtc ctgaaaggat taaacaagcg attgccagag aagctagtaa atacattaac
     7741 gattatgata cgctgtttat caattctagc tcgactgcgt tgaaaacact ggattttctt
     7801 caatctaacc atttgacagt cattacaaac aacgtgaaaa tcaattactc tccgcacaaa
     7861 gataattgta gttatatttt aactggtgga gagatgcgct ttccaaaaga agcactagtg
     7921 ggcgacatcg cattaaatac gatttcttca gtcaatgcag acatttgtat cattggctgt
     7981 agcggagtcg atgtagtaca tggggtcaca acaaagatat taaatgaatc aacgatcaac
     8041 gaaatgatgg tcaacaagac aatcaaatgt aagattttag tcgctgatca tcgaaaaatc
     8101 ggactgacat caaagttcaa gattgctgat atttcagtct ttgattattt gatcactgat
     8161 aaattttgtt ctgaaaaagt attagaggag atcagaaata cggggataaa aaccattcaa
     8221 gtagattcat tttaagaacc aggacgtaaa tatgtaaata ggattatcaa aaaaatgaat
     8281 aacaaattta tcgataaatg aaaaacacgc tacgctcaaa ttgataaagg aatcccaaga
     8341 tgtatccatc caatcgatac catcataatc tattgcttct tttataaccg ttatatgttg
     8401 cctaaatagg aatcaaacaa tatcgaaaaa aggaggtatg acaaaagtca cacctccttt
     8461 tttcgaataa acggtattca aatcagaaca ctgatttcac aaccacttta tttcaggtcg
     8521 ttttcaatca cctttaccgc gtaatcattt ccgttgaaac gcgctatttt ttttgcgtcc
     8581 atatatcttt ctaaataatc atcttctcct aattgcttat ggcaagaagc aatttcataa
     8641 agcaagtatt ccaatgcata agtcgattta tgtttcttat tatactcaat acccttctca
     8701 cataattgga ttgctttatc ataatcctcg attttacggt aataggtggc gatatcataa
     8761 tatacaatgc agaccatcag attatcaaaa tcagtctcac tcaatatttt aagtgtacgc
     8821 tccacataaa ctttcgcaaa atctaattct ccttttgaca agtagatcat tgccacgttt
     8881 gcagctgctt gtaagtgatg gatatctgtt tcggtcgttt gttccaacac ttgattaaaa
     8941 taaaataatg ctgcatctgg ttttttcgca taaagatgat caagcagtcc ttgataaaat
     9001 aaaatttgat ttaaaagatc agaattactt actttttcct gatcgatcgt ttgtaaaatt
     9061 ttataagatt cttggtattt tgatacagaa ttcaatgatt gaatctcatt tatttttttc
     9121 ctgattttgt cttcttctgt ttctactaat atatcggaga tctcgacatt cagctttttg
     9181 caaagcgcac ttaagatatt gatgcctggc gacatattct tgttttcgat attgctgaca
     9241 gttgcttgtt cacaaatgcc ttctgctaat tgtacttgtg ttaacccctt tttctttcgg
     9301 tattctttta gtaatgctcc atctacttta atcaaaacaa tttccttccc tttaaaaaat
     9361 tatttctcct ttatatcata cacaaaaaaa agttctaaac aaccaaaa
//
LOCUS       DADVXU010000100         9349 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.104, whole genome shotgun sequence.
ACCESSION   DADVXU010000100 DADVXU010000000
VERSION     DADVXU010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.104"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            34..1188
                     /locus_tag="KB953_002377"
     CDS             34..1188
                     /locus_tag="KB953_002377"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HBB6867315.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1210..1962)
                     /locus_tag="KB953_002378"
     CDS             complement(1210..1962)
                     /locus_tag="KB953_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBB6867316.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2326..4992)
                     /locus_tag="KB953_002379"
     CDS             complement(2326..4992)
                     /locus_tag="KB953_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBB6867317.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5333..6784
                     /gene="cls"
                     /locus_tag="KB953_002380"
     CDS             5333..6784
                     /gene="cls"
                     /locus_tag="KB953_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBB6867318.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            complement(7113..7928)
                     /gene="thiD"
                     /locus_tag="KB953_002381"
     CDS             complement(7113..7928)
                     /gene="thiD"
                     /locus_tag="KB953_002381"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBB6867319.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            8222..8497
                     /locus_tag="KB953_002382"
     CDS             8222..8497
                     /locus_tag="KB953_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867320.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            8924..>9349
                     /locus_tag="KB953_002383"
     CDS             8924..>9349
                     /locus_tag="KB953_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HBB6867321.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDL"
BASE COUNT         2820 a         1902 c         1634 g         2993 t
ORIGIN      
        1 taagtttttt atcataagta aagaggtgtt gaaatgaaaa tagggattga tcgtctttcc
       61 ttttttattc ctaatttata tttagatatg acagagttgg cagaaagccg tggggatgat
      121 cctgcaaaat accatatagg gattggtcaa gaccaaatgg cagtcaatcg tgcaaatgaa
      181 gacatcatta cactaggagc aaatgctgcc agcaaaatcg taacagaaaa agaccgtgag
      241 ctaatcgaca tggtcatcgt cggaacagaa tccgggattg atcattcaaa agcgagtgcg
      301 gtaattatcc accatttact gaaaatccaa tcttttgctc gttcttttga agtaaaagag
      361 gcttgctacg gtggcaccgc agctttgcac atggcgaaag aatatgtcaa aaatcatcca
      421 gaacgaaaag tactagtcat agcaagtgat attgctcgtt acagcttggc aagcggtggt
      481 gaagtgacgc aaggtgtcgg tgctgttgcg atgatgatca ctcaaaaccc gcgtatttta
      541 tcgattgaag acgacagcgt atttctgaca gaagatatct atgatttctg gcgtccagat
      601 tatagcgaat ttcctgttgt tgatggtcct ttatctaatt ctacgtatat cgaatcattc
      661 caaaaagttt ggaatcgaca taaagaattg tcgggtcgag gactcgaaga ttatcaggcg
      721 attgctttcc atattccgta tactaagatg ggaaaaaagg cattgcaaag cgtattagac
      781 caaacagacg aagaaaatca ggaacgtctt atggctcgct atgaagaaag catccgttac
      841 agccgacgaa tcggtaatct ttacactggt tcattatacc tggggctaac ttctctactg
      901 gaaaattcga aatcactaca gccaggagat cgcatcggtc tattcagcta cggttctggt
      961 gcagtaagtg aattttttac aggttatctg gaagaaaact atcaagaata tctcttcgca
     1021 caatctcatc aagagatgct agattctcga acacggatca ctgttgacga atatgaaacg
     1081 attttcagtg agacgcttcc tgaacacgga gaatgtgcag aatatacttc agatgtacct
     1141 ttttcgatta caaagatcga aaatgatatt cgttactaca aaatataata aaatcataaa
     1201 aagaaccctt cacgagtcgt gaaaggttct ttttttatct gcggaccaaa ggagcagtct
     1261 ataggtcagc ttagtcgtac taaggatttc tcgtttttca ctgaatagtt tgcgaatgta
     1321 cctggtcaca acaggatctt ttgcgtacgc gtcatagtat agcttcatca attcttcagt
     1381 aattgattcc tgtgtaaaat aagtaaaatc ttttttcttc actttgaatc cttcatcaat
     1441 taagtatgtt tcaaattgat ccattacaac tggcgatgta ttaggatctt cttctatcat
     1501 tcctagttgc ttttcgatag gataaaaaac atcatcgatt gattccacta ctctgattat
     1561 cacgcaccat ttgcttgact ttttcatcaa tttttctaat tgaataagat gtaccaatgc
     1621 tggatttgct gctgtaaaaa ccatttcata tgtctcattt gattggataa attcttctaa
     1681 cgactgtttg ataaaggaaa catttggcaa ctcatttttt tctgcttctt tttttgcatt
     1741 ttctagcatt cctgaagaaa aatcgatcaa atcatagcga ttaatttctt ttgctaagga
     1801 aagaaatctg ccagatcctc cagcaacgtc tgctgctgtt tcagcaggca ggactttctc
     1861 cgttttcaga taatttttta catcttcagc aatagttact tgcgattctt gctggatttc
     1921 ataatattct tcagcaaatt catcccattc tttttcattc atttgatttc ctctaatttt
     1981 ttgttatatg ttttcacttt tcggaataaa tcattctcct cttttatcta ttctatctct
     2041 tttgatgaat aataaaatga ttggctttcc gtctatcata aataaaaaaa gggactgtga
     2101 ccaaagtcgt gtcaaagtcc ctctttccga ataaacagtg tccaatcaga tgaccctcag
     2161 tattagctca aaatgctgat tttacaactt cttccttaaa cggtgttctc ctgccaactc
     2221 ttcggtcaca agtcacaata ttcaacaaac atgagaagct gtttgttgaa attgcacctt
     2281 gtgactcaga attaaacggc aggctcacaa cctcgttgat tcgttttatt tatttccttg
     2341 tactcgtaca atgatcaatt tagtgatttc catcaaaaca attgtcacaa gtgcgattcc
     2401 catacatgta aagacttctg tcaatccaaa tgctgatggg atagagaaga cttctttcag
     2461 tcctgggatc attgttaagc agtaaagcgc gctacataca actacagcta gtaagacact
     2521 tctgttagag aagaaccctg cttggatagc tgtttgtgtg tttgaacgtg ctgggaatgt
     2581 ttgcaatgtc cgacaccaaa tcaatgttga aaatgacatg gctacaccga attcaggtga
     2641 atgctgcatg ccaagccatt gagcaaagat aacagcaatc ccaatcagca caccacgata
     2701 cgttacagaa atcaatgttt tgtccgtaaa gattcctgaa tttggatctc ttggcgggcg
     2761 gttcatcgta gcaggttcag ctttttctgt acctaatgca attgctggca gtgaatcatt
     2821 gactaaattg atgaacaaca gttgtaatgc tgtaaatgga ttggaccagt cagcaactaa
     2881 ggcaaagatg atcgcaatga tcgcacctaa atttcctgca aatagataag aaatagcttt
     2941 cttgatatta tcaaacacat tacgtcctac ttctactgcg ctgacaatag aagcaaagtt
     3001 atcatccgtc aataccatcg ctgctgcatc ttttgccaca tctgtacccg ttcccatagc
     3061 aattccgatg tctgcttgtt tcaatgctgg tgcgtcattt acaccatcac ctgtcatagc
     3121 tgagatatga cctttatttt gccaagcacg aacgatacga attttattct caggtgaaac
     3181 acgtgcataa acagtgactt tttctaaaac ttcgtttagt tgatcttctg tcaattcatc
     3241 tagttccgtc ccggtcaaag ccaggtttcc ttcttcaaaa atcccgattt gttttgcgat
     3301 agcaacagct gttgttttgt ggtcaccagt aatcatgatt gttttgattc ctgcacttct
     3361 tgcttctgtt accgcctgag tcacttcagc acgtggagga tcgatcatag caagcaaacc
     3421 aacaaggatc aaatcgtctt catcttcaaa tgataaatca aaattatcaa gtggacggta
     3481 agcaaaagct agtacacgta atgcgcgtgt tgaaaaagct tcattttgct cttggaattt
     3541 tttcatcaat tcatccgtca tcggaacgac ttcaccattt agcaatactt ttttactgcg
     3601 ttggaagacg atatctggtc cgcctttaac aaacaagtaa tttttgttat caattgtgtg
     3661 tccagtagac attaattttc gatctgagtc aaaaggaagt tctgcttgtc ttgggtagtt
     3721 gtttcggatt tcctgataag gctggtttaa tttttcacta aagtcgataa acgctacttc
     3781 cgtagggtcg cccaatttag taccgtcttc actgatcgat gcatcattcg ctaaaacact
     3841 gatttcgatc aagcgacgtt cgtcagctgt ccattttgaa gggtcatttg tgaaatcccc
     3901 agtatcgcca ttacctaaaa agtagtcaac aactgtcatt ttgttttgtg tcaatgtacc
     3961 agtcttatct gtacagatga tacttgtcga accaagtgtt tcaactgctg gaagcttccg
     4021 aataatggca tgacgttttg ccattttatt cgttcccaaa gataacacga ttgttacgat
     4081 agactgtaat gcttctggaa tagcagctac ggctacggct acggcaaaca tcactgcgct
     4141 gacgatatct gcagtcatat ctcctgtacc ttctccaaga taaatacgaa acagttggat
     4201 acctaaaatc aataaagaca atgccaaaat agccaaactt agttttttac tgaatttatc
     4261 cagacctcgt tgtaacggag tgacttgctc agttgttgat tccaaaaggc cagcaacttg
     4321 tccgatctcc gtattattcc cagttgaagt cactaagaat ttaccgcgtc cgtaagttac
     4381 gatcgttcca ctgaatacca tatttgtacg atctccgatt ggtaccgtac ttgtgatatc
     4441 cgttacttct ttttctgcag aagtcgactc tcctgtcagc ataccttcat ctacttttaa
     4501 tgatcccgca tctagtaaac gtccatcagc aggtacataa tcgcctgcgt ctaacaacac
     4561 gatatctcct accacaagtt cgtttgccgg aatcgttttt tccttaccat cacgaatcac
     4621 tcgagcatcg ggcgctgaaa ggtttttcaa cgcttctaaa gaaccttctg ctttttttgt
     4681 ttggacaaca ctgattatag aattcaacat caatactgca aaaataacta agaattcgat
     4741 gtagtcaccc atgaccatct gaatcagtgc gacaatcagc aatacgataa ccattgcatc
     4801 tttgaaagtt tctaagaaca atttccaagt tggcttcttc ggcttttctt ttagctggtt
     4861 caagccatct ctttctaatt tttccttcgc ttgcgtggtg ctcaatccat cggttgagct
     4921 ctgcgtttgc tgcatcaacg agtcgatcgt ttctttatag accgcttgtt gtgcttgttc
     4981 cttgttttcc aaatttcatc ccccattttt tgatcttggc cctagtcctg tctgaaaaaa
     5041 aagagaccta agacaaatac gcatttgtct taggtctcac catttcatac aataccagaa
     5101 aaccatttgg ttaacttact gttgatatta tcgcaacaaa cgttgccggt tactccccta
     5161 aggagatatt caactgtact aatatcctac cttattaacc tacaaaaagc aactacttta
     5221 gcccgattta cctacatttt catgaaagga gtgaaaagta tattcacctt ggtaggcttc
     5281 tttcttctat catgctatac taatgttatt gtatgtaagg aggaacattt ttgtggtatc
     5341 tagtattata accgcccttt atctattaaa tgcacttatt gctttggtgg ctattttgat
     5401 caaaccccga gatgtagcag ccatttgggc atggctctta gtattgtttg cccttcctgg
     5461 ggtgggcttt gtattatatt tatttttcgg acgtggatta acggataaga aaaaattcta
     5521 tctccgacaa agtgacttga aagaattaga aaactttcag tcttttagag gagataccat
     5581 tgaacattac gatcctgaca tgggcgataa agacaaacag caatttgttg acttcttctc
     5641 ttcattaaat cgtatgccgc tgacaagaat gaattctgtc actcttctca cagacggaca
     5701 agagaaattg gattcactgc ttcaagatct aaaaaaagcc aaacattcga tccatatcga
     5761 atattacgca tttgtgacag ataatatcgg ccagcaagtc ttacatgttt tagaagaaaa
     5821 agccgcagaa ggcgtggaag ttcgaatatt atatgatgca tttggctctc atggcacaaa
     5881 agcaaaagat ttcaatcgtc taatcaaaaa tggtggacat gtccatacat ttgttacctc
     5941 acaaagggca ttacttcgtt tccgattgaa ttaccatgat caccgaaaaa tcgttgtgat
     6001 cgatggaaag attagttata ccggtggttt caatattgcc aatcaatatg taaatacaac
     6061 aaaaaaattc ggctattggc gcgatacgca tatacggatt ttcggtgccg cttcttcttt
     6121 gctccagctt cgcttcttaa cagactggaa cgtctcggta cctgaagaaa aaaaggtcgg
     6181 ctatcatttg aattatttct ttaaaaaagc agatcgagat gaatctaagc ttgctgatac
     6241 atccatccag cttgtttcaa gcggaccgaa taacgaaagg gaacaaatca agctttcatt
     6301 tatcaaattg attacttctg ctaaaaaacg tgtttggata cagacacctt accttgttcc
     6361 tgatgaaagt gtcattgctg ctttaaaaat cgcaactgcc tctggtgtag atgtgaaaat
     6421 catgattccc aacaaaccgg atcatccttt tatttatcga gcaacacaat attatgctcg
     6481 gcagctgatc aaggaaaatg tacaaatcct tgtctatgag aacggcttcc tccatgcaaa
     6541 aacattgata atggatgatg aaatctgcat ggtaggttca gcaaatcaag atattcgtag
     6601 ctaccgattg aattttgaaa caagtgctgt catttacgat cctgagtttt tagaagaact
     6661 tgctactcag ttcaaagaag atgagacaca ttgttcatcc atgacaactg aaacagtcaa
     6721 ggaaatgtct aactggctat tattcaagca acaaatttct cgattatttt ctccaatcct
     6781 ataatttttt tattcaagta gagaaaaata tactaagatt aaagggactg tgacaaaaat
     6841 cttgtcacag tcccttcttc cgaacaaacg gtgttctcct gccaactcct cggtcacaag
     6901 tcacaatatt caacaaacat gagaagctgt tttttgaact tgttccttgt gactcggagc
     6961 tggacggcag gctcacgacc tctttcttaa acggtgttca aaagcttgca tctgcggtaa
     7021 taagtccaac caagttaaaa acataagaag ctgtttttac ttggctgttc ttattacttg
     7081 atgcagggca actttttcac aacttctttc ttttactcta attcaccagc tgcccaatga
     7141 ttgatggggc catatttgtg ccctacattt atttcttcac gaattgcacg gtttacatag
     7201 tcttttgcca atcggatcgc ttgttcaact gtttttcctt tcgctaactc agctgcaata
     7261 catgcagata gcgaatcgcc agttccgttg atccggtcgg taggataaaa tggtgcattt
     7321 aaccaaaatg attttcctga ttcgaacaat acgtagtcat tgactgtttc ttgctgcgca
     7381 tttttagcat gatgtccttt gatcataacg ttttttgcac ccatatcttg cagttctttt
     7441 gctgcttgca caaaatcttc ttcagctgct aatgtccgtc cagtcagctt ttctgcttca
     7501 taaaaattag gtgtcagtac ttcggccaaa gggacaagct tttctttcaa gctttgcaaa
     7561 gcagattctt ctagtaaaag atttccatgt ttcgttacga ttacgggatc cacgactaat
     7621 ggtccaaaat catatttttg gtaatttttt acaactgtct caatcaactt tgaatctgct
     7681 agcatacctg ttttagctgc acgtatttga aagtccttcg caatcagttc aaattctttg
     7741 tcgataaaat ctgttggcaa tgtcacactt gctccgattc cgtaagaatt acctgcaaca
     7801 catgcggtca tgacagaaac accgtaaaca tttcttgtaa agaatgtatg catatctgct
     7861 tgcatccctg cgcttccatc gctatctgat cctgcgatcg tcattgcttg tacatactga
     7921 tcttccatca aatcgattcc tccattttta taaaaagatt tttcataata ctgttactgt
     7981 ttctatcata tgtttttcaa agtatccctc agtaaatata ccactacctt ttcttggtaa
     8041 tcaaatttat ttgaaaaaac atttgcaata aatcagaaaa tagtggtcgt ttatttgtat
     8101 tcgctattct aataataaat aaccactatt tgatttatat atatttcagt atccttcttg
     8161 ttttcatata accaaaggcg ctatgatttc catatgaaaa aaataaagtc aggtgaaaaa
     8221 aatgaaactt gaggataaaa aagtcatttt gaagaaaatc aaacgactac cagaccaaat
     8281 agatggctat acgatctgtt ctttcaaaaa agacaggagt ataaaagctt atacgcaaaa
     8341 cacagaaaaa ggaataatct atttcctaca tgaagaaggt tacgataccc aaacctttat
     8401 gtttactgaa aaaaaagaat ttcataaaca gatgaaaaaa ctgattgaaa aagaatttcc
     8461 aagaagtcat aaactctatg tgaatcagca attttaactt ctaactgttc tttattaatt
     8521 aaaaatgttc tgtaataaaa taaaacttta taacaactat aacaaaaccg aatgcaaaac
     8581 agaagttcta taagttataa tcatagttgt taggtgattc atctagtaaa aacaccagat
     8641 ttcaaatttc acacaattta aactagatca atcatcaacc ttcttcaaat tgttagaacc
     8701 ctttcccgag gagctgcagc ataagcaaaa aaacaaacag gaagccaaat ggctaagaca
     8761 ttttttctat tagtctagtt gcttctcgtc gcaagtttaa gactttagaa agttatttct
     8821 aatgatgttt gggttgtttt gatcaatgct tatgcacagc tccttaccta ttatttatct
     8881 caacagatct aacaaattaa tggactattt aaggagtttt tttatgctta cgatctattc
     8941 ttctcgcaca catagttacc atctgatggc aaactggctg aaagaccatc aaatgcctta
     9001 cagagaacga attttcacac ctaaacagcc tttaacaagg aaagagatat tttatttatt
     9061 gtcattaaca gaaaacggat ttgacgatct attagctaaa cgctcgattg attataaaaa
     9121 tttgaaagac caaatcgacc aatgtacgac ctcagagctt gtcgatctga tcgtctcgca
     9181 ttttactttg ttacaacgtc ccatcattac tgacggaaag aaattattag tcggattcaa
     9241 tgaagatagc atgcgtcttt ttatccctgt acaatggcgc agaagtcaac tgaaatctgt
     9301 ctataacgat tttacatggg taagcaatct agaaaattct acggattta
//
LOCUS       DADVXU010000101         9293 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.105, whole genome shotgun sequence.
ACCESSION   DADVXU010000101 DADVXU010000000
VERSION     DADVXU010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.105"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(24..692)
                     /locus_tag="KB953_002384"
     CDS             complement(24..692)
                     /locus_tag="KB953_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBB6867322.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            652..847
                     /locus_tag="KB953_002385"
                     /pseudo
     CDS             652..847
                     /locus_tag="KB953_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309529.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            826..1113
                     /locus_tag="KB953_002386"
     CDS             826..1113
                     /locus_tag="KB953_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867323.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1529..3067
                     /locus_tag="KB953_002387"
     CDS             1529..3067
                     /locus_tag="KB953_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HBB6867324.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3064..4029
                     /locus_tag="KB953_002388"
     CDS             3064..4029
                     /locus_tag="KB953_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6867325.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4016..5338
                     /locus_tag="KB953_002389"
     CDS             4016..5338
                     /locus_tag="KB953_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBB6867326.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5401..5505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5579..6322
                     /locus_tag="KB953_002390"
     CDS             5579..6322
                     /locus_tag="KB953_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6867327.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6337..7329
                     /locus_tag="KB953_002391"
     CDS             6337..7329
                     /locus_tag="KB953_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBB6867328.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7341..8078
                     /locus_tag="KB953_002392"
     CDS             7341..8078
                     /locus_tag="KB953_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6867329.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8068..8790
                     /locus_tag="KB953_002393"
     CDS             8068..8790
                     /locus_tag="KB953_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBB6867330.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8874..>9293)
                     /locus_tag="KB953_002394"
     CDS             complement(8874..>9293)
                     /locus_tag="KB953_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HBB6867331.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3044 a         1603 c         1965 g         2681 t
ORIGIN      
        1 aataaagtag tgaaaaataa gaatcagtgc tctaactgtt caagaggatc aagtggttca
       61 aatgtttctg tagtctgttt tgcttccaat tggggcaaaa cagatccaat ttccagatac
      121 ttcactttat tcaatgtata agatagaata gaacagctaa gtaatgtata cagaaaatgt
      181 gtgagatcca aataaagcaa ggagacaact aaaataccaa aagtaatcaa gaaaagactt
      241 ttgccaatct taatatgaaa atatttttca atggcttgat aaatgatatc tgttccgcct
      301 gtgcttccgc cgaatcgaaa gacgagaccg caaccaatgc cagaaagaat acctgtcatc
      361 aaactattga tcagtagact atctacagca aaatgtatat gaaggctctc ccataaatgt
      421 aaaaagaaaa ctagcgcaaa tgttccgacg atcgtccgga gaaaagcgtg ttttccaaaa
      481 agaaacaatg agataagcaa taaaggtaaa ttaaaaagca gtgaagtgac agaaggagcg
      541 aatccaaaaa gattatgcaa aagcaaagca ataccagtaa ccccaccatc tgccagctta
      601 gatggaatat tgattcctgt cacagctaat ccgtagattc ctgcgcctaa aatgattccg
      661 cacaattctg taaataaatt taattttttc atttctagcc tcttttcttt tgaaaatttt
      721 cagcaacttc tttattatac tgacttatac tcttacttca agaggaaaat gaaaaaaaat
      781 tatttttggc tgtttctttt tatttatcaa ataaggagtg gaaaaatgcg agcagcagac
      841 ttattgacaa ttcttacaaa atcacccaaa aaaacagagt atcaaggagt ttttttggct
      901 caagaacaaa aactggtgcc tgtgacaatg gtccagataa ataaagcaaa tcaatttatc
      961 tttttatttg aacacggcaa agcaccactt gctatgaaag acatctttct ttttttgatg
     1021 aagaaccgtg agaaagctgt ttattatcag tcaaaagaag aactcattcc tctttatgga
     1081 gtgaaagaga tagaaaataa agtggtgata taagtaaaaa taagaaaaca agacgtttat
     1141 gatgtcaaaa caaccatttt ttacattttt ggcaaaaaag tgtcatgata cagtataata
     1201 ttcgttgaac aggccaataa aggtgttctc ccccacgaga tttctacttt attggctgtt
     1261 tctttttatt aaaataaaag ttgtgtgtgt aaaaaagata tagctgattc caagtgacaa
     1321 tttcgtatag atttttatgg tattcgtagt gaaaaaacga gaaccaaaaa gttgatccga
     1381 ctttgttgct tggattcttt ttttgtcagc atatttagag gtattttttt cttttttttg
     1441 ttcgtgctgt aaggttaagg caagacaagt tagttgggaa aaaggaaaga aatgtttata
     1501 cttaaagaat aacagaaagg aaggtcgcat gaataatttt atacaaacat taagtgaaag
     1561 aaaaggcgag ttgctggaag ctgcatttca acatctgtcc atttctctca tttctttgct
     1621 gattgcagca ctgattgcta ttcctttagc gatttgggca gccaatcata aaaaaatggc
     1681 agaagtctta ttgcagatca cgggtgtgct ccaaacgatt ccttcacttg ccttattagg
     1741 tttacttatc ccatttgtag ggatcggaac tgttccagca ttgattgcac ttgttattta
     1801 cgctttgctt ccgattttcc aaaacacgta tattggtcta gctgaaattg acccgtctat
     1861 tgaagaagca gcggtagcat ttggtatgtc cagaatgaga cgcttgctga aagtagagct
     1921 tcccatcgca ttacctgtca ttatctcagg tatccgaact gccttagttt taatcatcgg
     1981 tacggctact ttggcagcat tgatcggagc aggtggatta gggacattta ttttgttagg
     2041 tattgaccga aatacacctg ttttgacatt gattggtgcg atcagttctg ccttgcttgc
     2101 aattatattc agcggactga ttcgctggtt gcagcataag aaacctcgat attccatcat
     2161 tactttagca gcagtggtgt gtttatttgg cggcgtttct ttatatcagt ctgccttgtt
     2221 taaaaatgaa accatcacga ttgctggaaa attaggagcg gaacctgaaa tcctgatcca
     2281 aatgtataaa gaattgattg aagacaacac aaaaacagaa gttgaattaa aaccgaattt
     2341 tggcaagacg agttttttgt tcaatgcact ggatcaaggt caaatcgata tctatcctga
     2401 gttcacaggt actgtcctag aaagcttggt gaaggtacct gaagcaatga aaagtgctga
     2461 tttgacaaaa gaaaaaacat atgacgaagc agaagaatta ttgaataagc aattcgatat
     2521 gacattatta aaaccaatgg cttaccaaaa tacgtatgcc ttggcagtca agagatcttt
     2581 tgcgaacgaa catcaactta aaaagatttc cgatttggca gctatctctg atcagatcaa
     2641 agcgggattc acactggaat ttattgatcg ttcagatgga tataaaggaa tccaagaagc
     2701 atatggcttg aatttttcaa gtgtacaaag catggagcca agtttgcgtt accaagcaat
     2761 caataatgga gatgtcaata ttgtggatgc ctattccaca gatagccagc tcaaggagta
     2821 cgatctagtg actctagaag atgatcgagg gttattccca gcttatcaag gcgcaccgct
     2881 gatgaaaaaa gattttgcta aagaacatcc tgaaatcgtt aaagcactga ataaattaag
     2941 cggaaaaata acagaggatc aaatgattga tatgaattac caagtcaatg tcgaaaagaa
     3001 aaagccgaca gaagtagcac gaatcttcct gaaaaatgaa ggattagtaa aggaggaaga
     3061 tcagtgacta aattaatcga gttcaaacat gtgcaaaaaa agtatgatgg aaagtatgtc
     3121 atcgatgacc taaatctggc aatcaaaaaa ggagaaatct ttgttttggt tggtccttcc
     3181 ggtagtggaa aaacaactac tttgaaaatg atcaatggat tatctaaacc atcagcagga
     3241 gacatctact tcaaaggaaa atcgttaaat gaatataatc tgcagaaaat gcggtggaat
     3301 atgggatatg tgctgcagca aatcgctttg ttcccgacca tgacagtcaa gcaaaatatt
     3361 gaagtcatcc ctgaaatgtt aggttgggaa aagcagaaaa gagcagaccg tgtagatgaa
     3421 cttcttcaaa aagttggctt atctcctgat atttatcgag atcgtatgcc tagagagttg
     3481 tctggaggag aacagcaacg gatcgggatc atccgagcta ttgctgcaag cccagatgtg
     3541 attttgatgg acgagccatt tagcgcatta gatccgattt caagaaactc attacaagaa
     3601 ttggttttgt ccttacacga agaattgggc acgaccatcg ttttcgttac gcacaatatg
     3661 gaagaagcaa tcaagctagg tgatcgtatt gcatttatga aggatggaga aattatccaa
     3721 tgtgacacac cagagcagtt attgatgaat cctaaaaacg actacgttcg gcatttcttt
     3781 gatgaaccga aacagacgaa agagtggcga gtagaagatt tagtcgtcaa tggatatttc
     3841 ttaaatgaaa taccagaaaa tgctcgtcaa caagtttgct ttgatacgcc tatgaaagaa
     3901 gtatattcgc ttctttcggt gtatccaagc attgtgatag tagaaaagca aagaagtatt
     3961 gggtcgctga caagtaaaga aatctttgct tttttaagtc gagaggagga aggaaatgaa
     4021 aacatatgat gcaatcatca tcggttcagg tgtgagcggc ttaagtgctg cttatggatt
     4081 gaaagaagca ggtaaaacag tactagtggt cgaagaagat ctatggggcg ggacttgtcc
     4141 taaccgtgga tgtgatccta aaaaagtctt gttaagtgca gtagaggcac gtaatcgggt
     4201 aaaacaattg agtggaaaag gttttaacga gatcccaact gccaactggg aagaacttca
     4261 aaaattcaaa cgaacattta cggatccagt cccagaaagc cgaaaaaaac aattagcaga
     4321 agctgaaatc gatcatcttt caggaaccgc tcgttttttg gatgattcat ctattgaagt
     4381 caacgaagaa gtcttccatg cggattactt agttttggca accgggcaaa gacctacgat
     4441 cttaccagtg gagggaaagg aatacttgaa aactagtgca gactttttat cattgcctgt
     4501 actgcctaaa gaaatcattt ttatcggtgg tggttatatt gcttttgagc tagctacgat
     4561 tgccaatgct gcagggagca aagtaaccat tgtccatcat aatcaacggc cattaaaaga
     4621 atttgaggcg tctttggtag aggaagccgt ccatcaaatg gaagcatctg gcattcagtt
     4681 tgcttttggt gttgaaaccc aaaagatcat ttcagaaggt actcgctatc gtttagtcgg
     4741 aaaagagacg gaattagtag cagatatgat tttttgtgcg actgggagac agccgaatac
     4801 agaatcttta gcacttgagc aagcgaatat cgtctttgat aaacacggaa ttgcagtcaa
     4861 tgattatctg caaacgagca atccaaaaat ttttgcgtgt ggagatattg tttcaaggaa
     4921 aaccccaaaa ttgacaccag tcgccacttt tgaaggaaat tatgtagcta agcggataac
     4981 ggatgcaaca agcgaaccta tcaaatatcc gattatacct acgatcgtct acgccagtcc
     5041 taaacttgct gaagtaggag ttacaaaaag tcacgcctct tcgtcagatc aagtggtaga
     5101 gatggatctt accagttggt tcacctatca tcgtgtcaat gaaccagttg ctaaggctga
     5161 attgaccttt gaccagcaaa attatttgat tggtgctgca gtgattagcg agcaagcaga
     5221 cgaattgatc gatgatttga cactcgtcat caatcaaaaa ttgactaaaa aagagttgga
     5281 tagctatatc atggggtatc cgacattggc aagtgatctc tcctatttat tgaaataatc
     5341 agcatgataa ctaaagcgct tgttgctttt tccttcttgt gctacacttt aaaaaatgaa
     5401 tacctgtttc tggggatgtc gtagacgaca tgagagtaag ttgtgaactt agacccattg
     5461 aacctgtcag ttaatactgg tgaagggaga aacatagcag acgcttgaag cgggttacta
     5521 tgtcgtgccc atttcaagcg ttttttgtta ttcatattag aagaaaggag gtaacaaaat
     5581 gaagaaaaat ctttatccag cagtcagtat ggcattcatt ttaattattt ggcagattgt
     5641 tgtcatagtt tggaaaatac ctgcctttat tttacccgga cctgctgctg tattttctgc
     5701 attagcggca gatgcacaag tattactttc ccattctgtc acgacattaa atgaagcagt
     5761 gcttggtttg gtaatagcta gcgttctttc ttttattact gccttggcaa tggattattg
     5821 gaagttttta gaaatgtctt tttatccgtt actagtgatt tcacaaatgt tgccaatcat
     5881 ggtgttaggg ccattactta ccctatggtt tggtttcggt atgcttccaa aagtgatcct
     5941 tgttgtcttg ataagctatt ttccgatcgt agtttctttt tcggatacat tgaggaaagt
     6001 atcaaaagag caaatcatct ttttgaaaac gatgggggca gatacaggga agatttatcg
     6061 aatatataaa ataccagcgg gtttatccgg cttcttttcc ggtttaaaag tggctgctac
     6121 ttattgtgtc agcggagcag tcgttggaga atggctaagc gctcaagcag gcttagggta
     6181 ttacatgata cgcgtaaaaa acagctacca gatcgataaa gtttttgctg cgatcatttg
     6241 tgtcattcta ttgagtttat tgctgaatgg cgcttgttca ttattgaaaa aagggtatta
     6301 tcgtatgctt tatatttttt aagagaaggg gatcaaatga aaaaattatg gcttttatta
     6361 ccgctactgt tgcttttatc cgcttgtggc actgcaaaag aaacaagtag caaacaagaa
     6421 ataaaggatt tgaaaaaagt gacacttgtt ttggattatg taccgaatac taatcatact
     6481 ggcatctacc tagcaaaaga aaaaggctat tataaagagg ctggtttaaa tgtccaaatc
     6541 atcgaaccag gagataattc gacaagtatt ggtctagtag gtgcggacaa agcacaattt
     6601 ggtgtaagtt atcaagaaga tgtcacttat gctcacgcag atggacagaa tataccagtc
     6661 aaagcaattg ctacagtgat caaacataat acttctggat tcgccaccct atcagacagt
     6721 aacatccatt caccaaaaga ttttgaagga aaaacatatg caggttggca aagtccaagt
     6781 gaagaagctg tcttaaaagc tgtgatggaa aaagacggcg gggacttttc aaaactgaca
     6841 atggtaggaa gcaatggaga agggccagaa agcttaggaa aatcctcaga tatccaatgg
     6901 tactttgaag gttgggatat gatcaaagca aaagaagcgg gtattgaact aaactatatc
     6961 ccattgaaag aattagatga acggttagat tactatactc cggtaattat tacgaatgat
     7021 caactgatca aaagtgatcc agaattggta cagtctttta tggatgccac gaaaaaaggc
     7081 tatcaggaag ctattaagga tccaaatgac agtgctaaac tgttgcaaaa atatgcaaaa
     7141 gaaaatgatc ggacgttttt agaagaatca caagcattct tgtcaaaaaa ttatacggat
     7201 gatcctgaga attggggatt gatggaagaa aaagtttggc gcaattatac agcttttatg
     7261 caggaaaacg gattgatcaa gcaagatgta ccttcgcaag aactattcac taatcagttt
     7321 ataaaatagg agtgatagat atgtcaatcc aaatcagcca cttgaatgta gtcattgaaa
     7381 acctgaaaat attagaagac atccatttca ctatccctaa agataccttt acatcgattg
     7441 tcgcgccaag cggagccggt aagtcaacat tgctgaaaac tttaacagga gtccagccta
     7501 tgacttctgg aacgatcaaa gtagacgatc aaaaagtaac tggtctccat acagcattta
     7561 gttatatgcc gcaagaagat atgttacttc cttggctgag cgtctatcaa aacgttacgt
     7621 tatatcaaaa aatcaatcat ctaacaatca atgaagaaca agtaagggat tatctggata
     7681 tctttgggtt aaacggctat gaacattttt tgccggaaca attatctggc gggatgaagc
     7741 agcgaacggc cttgttacga acaatcatga atccttcttc ttacttgctt cttgatgaac
     7801 catttggagc gttagatgct atgaccagag ggcagatgca agactggatg ttgaagctgc
     7861 ctaaaaaagc aaagcggact actttacttg tgacgcatga tatcgaagaa gcaatttatt
     7921 tatcagacag gattcttgtg ctttccgccc gtccggcaca tgtgattgct gagattcaag
     7981 tgcctgaaaa acgacggtca cgtgaatggc tactgcagca atcagagttg aagcaaacga
     8041 tttatcagtt attggcaggt gaattagatg ttaagtgatg ctcattgtca tttagatggg
     8101 agtcagagtt tagcactcct tcagcaggaa cacacgattt tgacgatcat caattgtgat
     8161 tcgccagaag aatggaagga aaacagacag cttgcagcaa gtaagactca agccttaagt
     8221 tacggtatcc atccttggaa agcagatagc tatacgtttg aacaagttga gccttttttg
     8281 aaaaaagcaa ggatcatcgg tgaaataggg ttagataata tttggacgaa tgtaccgatg
     8341 actacacaaa aaaaagtatt tgagcgacag cttgcttttg cagccataaa tgagaaacct
     8401 gttgtactgc acacaaaagg atgcgaaaaa gagatcttaa attatatcca agcttaccct
     8461 aatcactatt tgatccattg gtattcttct ttggactatc aacaagacta tattgattta
     8521 ggctgttatt tcagcattgg tcttgatttg aaaaagaatc cagcagtctg gcaactagct
     8581 aaagcagtcc caatcgacca cttacttatt gaaacggatg gactacatgc agcagagtgg
     8641 gctttagggg ggattttttc agaaaaagat tatgtctcac tgcttgaaga acagttaaag
     8701 acaatcgctg agatcaaaga gctatcagtt gaagaagtta aaaaaaatac ttatcaaaac
     8761 ttggaacgtt tcattagatt aggtaaataa aaaagagatg tgacacaact agtgtcgcac
     8821 ctctttttgg ctttctttgt ataaaagtaa actgtttgat taataaatga atcttagtac
     8881 caaccgtttg cttcccagaa tgctttcgcc gcttcccaag aaccataacg tgaagtaaca
     8941 tattgttctg ctactctttc ttggtttgca gcagagtagt caccatttaa gtaagaagaa
     9001 tctaattggt aacgtccgat gtaacgtccg ttcgttgctg tgtaagaacc gcttgattct
     9061 ttttgtgcga tccattcttt tgcagagctt gtgcttgctg gagtagcagc agtttctgtt
     9121 acagcaggag caggttgtgt ttcagcaaca ggcgctgctt gtttttccgt ttgaaccgtt
     9181 tcttgtacaa caggttgttc agcttggact ggtgcttctt gaacggctgc ttgaggttca
     9241 gcagctgctt gtgcagagcc ttctgttggg attgttaatt gttgtcctga gta
//
LOCUS       DADVXU010000102         9070 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.106, whole genome shotgun sequence.
ACCESSION   DADVXU010000102 DADVXU010000000
VERSION     DADVXU010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.106"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..371)
                     /locus_tag="KB953_002395"
     CDS             complement(<1..371)
                     /locus_tag="KB953_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867332.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLS"
     gene            complement(668..3049)
                     /locus_tag="KB953_002396"
     CDS             complement(668..3049)
                     /locus_tag="KB953_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HBB6867333.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(3039..3686)
                     /gene="recU"
                     /locus_tag="KB953_002397"
     CDS             complement(3039..3686)
                     /gene="recU"
                     /locus_tag="KB953_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HBB6867334.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3750..4289
                     /locus_tag="KB953_002398"
     CDS             3750..4289
                     /locus_tag="KB953_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HBB6867335.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4379..4798
                     /gene="gpsB"
                     /locus_tag="KB953_002399"
     CDS             4379..4798
                     /gene="gpsB"
                     /locus_tag="KB953_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HBB6867336.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4820..5187
                     /gene="rnpB"
                     /locus_tag="KB953_002400"
     ncRNA           4820..5187
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="KB953_002400"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5396..6562
                     /locus_tag="KB953_002401"
     CDS             5396..6562
                     /locus_tag="KB953_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HBB6867337.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6608..8104
                     /locus_tag="KB953_002402"
     CDS             6608..8104
                     /locus_tag="KB953_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HBB6867338.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            8185..8868
                     /locus_tag="KB953_002403"
     CDS             8185..8868
                     /locus_tag="KB953_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBB6867339.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         2896 a         1619 c         1898 g         2657 t
ORIGIN      
        1 gataataaaa aaataccatt tataactaaa cataaactaa tggatttggg cggtttcagg
       61 ctcatcagaa aaatatagat tctggtgaac aacaaccata aaaaagtgag aatcattgca
      121 tgagagcttc tttccataaa ttttccttta agagtagtgc ttaaaagtac agaaaaaata
      181 ataaatgcaa ggataggagc aattaaagta aatttattta ataataaaca aaatttttca
      241 gcttttttat tcttggaata acgaggtatt gttttcgata aaaaaagatg gtgcagacaa
      301 attatgataa ttaagcatac tatcagataa ctcaaaatga agccttcttt caaaaaatta
      361 ttcaaaacca tatattgtat tttttatttg ataataacaa tgggattatt aagaagtggc
      421 actaaataaa agactagtgt gtatttagcc gatttaatta taaacaagta aatggataaa
      481 gtatatgtaa tgataaaaga aaaatggatt ttctaatttt taagctaaat gttagatcac
      541 acatgtatat gctggatagt cttataaaaa atcatgtaat acagcttggt acatgatttt
      601 tatttgtagc tgttttttac atagacacaa aaaagaaacc acgcttgtag acgtggtttc
      661 ttttttacta tctgtttcca gaagctgagg tgctgctgga tggtgtatgg tcatttgcag
      721 catccgctcc gctagttgct ggctcactgg aagaagcagg aggagcagat gaagacggtg
      781 tcgagctttc ttcggatgaa gaagaaggaa ccgttgttga agacgctggc tcgcttgttt
      841 ctgctgtcgt gctttctttt gaggactcag cagttgattc gctttgacta gtagagctag
      901 agctttgagt cgttgtctct gttgttgagc ttccaggagt ctggacatag ctggaagatg
      961 gaatagttgt tgatggtgta attgcattcg atctagctgt atattgatct ttgtagtaca
     1021 gttcaccgcc cacacgaatt aatccgcttg gcatctccca gtctgtattt gtcacgttag
     1081 ctgaaacata ctgcataagc tcgcgataaa catcagaggc tacgtgtgac gattctgaag
     1141 tgacaggcgt cattttttta ttgtaacctg tccacacgga aatcgaataa tttggcgtat
     1201 agcccgcaaa taggatatct ggatacaccc cagaagaaat accaagtttt gcatactcgt
     1261 catctgtgta attggatgtt ccggtcttac cggcttgata taacccagcg atttgcgcat
     1321 tcgtaccagt tccttcagtg atcgtatctt ttaaaatgtc tgtgatcata taagctgttt
     1381 ctggagacat ggctgttttt ccatctggct cataagtttc ttctgttccg tcttggaaga
     1441 cgattttatt aacatattga ggtttgtaat aagtccctcc attggcgaaa gccgcatagg
     1501 ctgctgccat ttttaaggaa gaagctccgt attttgtccc atcttgttct tccgtattac
     1561 tagagatcgc attcgattga tggatcgtac tgtattcgat cccaagattt tttaagaaag
     1621 aagccacttt atctgaaccg acttcattga ataatttgac tgctggaaca ttacgggata
     1681 agtaaagtgc ttggcgtaaa gtaatggtgc ccatatattg attatcccag tttcctacag
     1741 gagtcgaagt cccttcatag ttatacggtg cgtcagtaat agtttttcca gtagaatatt
     1801 tcaaatattc aaaagctggt ccgtaatctg tgacaggttt catcgtagaa ccaaaatcac
     1861 gggaagtatt cactgctaag ttgttgccta aagttacgtc ttctgcgatg tgacgtccgc
     1921 caatctgggc ttttactttc ccggtattgg tgtcgataag ggtggaggca acttgcatct
     1981 cgtcgtctgg gtaagagaca tactgatccg tgttgacgat atcatatagt ttcttttggg
     2041 cgtccagatc caaattcgta taaatctcta aaccgtctgt gtagacattc ttatctgttt
     2101 tttcttgtac ttcattgatg acttctttga cataattatc gacgatctta gtattgtcat
     2161 cggattgagt tagttcttgc agaccatccg ttaccggtac attaacagct tgatcatatt
     2221 ctgtttgaga gattttttcg ttttgaagca tcgtatacaa aacagtatct cgtcttttct
     2281 tcgcctgatc tggataaaca taaggatcat aggcggaagg ggcttgaggc atcccagcaa
     2341 gcaatgctgt ttgcggtaaa gaaagttctg ataatttctt accaaagtac atctcagaag
     2401 ctgtctccat cccgtacaag ccgtttgaca tatacacttt atttacgtag taggtcaaga
     2461 tctcttgttt cgattttttt tgttccaatc ttacagccat ccacgcttct tgggccttgc
     2521 gtttcagagt ctgatcctcg gcactggtgg agaagaagga aagtttgatc aattgctgtg
     2581 tcaaagtact tccgccttgc agtccgccag aggtaaagtt agagagggca gaaccgatga
     2641 tccgaatagg gtcaaccccg atgtgtttat aaaaacggcg atcttctacc gacacgatcg
     2701 catcttctaa tgttttcggt aattcattcg cagaaatttt ttcacgcttt tctgcgccta
     2761 aatcttcgaa tagttcgcca tcttgtgtat atagtttgga agatacggta gcgtctaatt
     2821 ttttatccgt caattcgggc gcatcttttg cgtaatacca gaaaagtcct acacctgcta
     2881 aaaagaggat acagaaaaat gagagtattc ctaaaaggat cttgataaat aatccttttt
     2941 tatgtgttcc agaggacttt ccagaacctt tgccagaatt cttcttcaca ctctttttag
     3001 gagtaggctg tttctgtcgt ctagagcttc tagtttgttc atttgccatt cttgctttct
     3061 ccttcaatag ataatgttga taaatagaag tcaaccgcgt ccaaataagg aatccgcgga
     3121 gaaataccag ttttgatctc tattcctagt tcttcgatca aagataaagg tagtgatttg
     3181 cgtccttttt ccttttgggc gtcccagtaa gcaatcaaat gaacactatt caaataaaaa
     3241 cagcgcttca aagacgaaaa ccataacaag acaaagcaga ccccattttg ttcgaggcat
     3301 gctttcatat gttcgatttg atgggcatgg aagtttttaa agggaaaaga tgttttgttc
     3361 ttggtttctt ttgcttcaaa gtccaagtaa aagcctcgat agacgccatt atagtctgta
     3421 gttgaggctt gggtaaagta ggcttccttg atcacagcag cacttctctt aggatagtct
     3481 actttcacga tttggatagg ggtaggcttt ttatggataa ccgccaaccc gcgggaaaga
     3541 taatatttat tactttcatt gattgcttct tcaaagcgca tcccccgatt gccgaattca
     3601 gtgatctgat ttcttttttc ttgaattttt tcgacgggag aggcattctt ataataaggt
     3661 tggccatttg gatagcgcaa taccatcttt aatcacactc ctaggccaca ttatagcaaa
     3721 aaagcggata gaaagaaaag gaatctgaga tggataagtc aaaagtaatc tatgtgtctg
     3781 ggtatcgaag tttcgaactc ggtgttttta aagaaaatga tcctaaaatc caagttgtta
     3841 aaaatgtttt aaaaaaagaa ctccatcagt tagcagaaga agggctggaa tgggtacttg
     3901 tcagtgggaa tttgggcgtc gaactatggg ctgtgcaaat agtagcggaa ttgaaagaag
     3961 aatatccgga actatctctt ggtattttgt atcctttttc tgattttggt aataactgga
     4021 atgaagggaa ccaagaaaaa aaacaattag cagaacaatt agcagattat gtcgaagctg
     4081 ttagccatca accctaccaa tcacctagtc agctaaagaa tcacactcgt tttatacttg
     4141 agcatacagt gggctgtctg ctgatctatg atgaagaatt ccctggaaaa acgaagtatt
     4201 tttttgaaga tgcacagaat taccaagcag atcatcccta cgaaattcgg ctgatcagta
     4261 tggacgatct tcaaaatttt agtgaataat cgatattgtg tttgatttat caactttttt
     4321 cctatacaat gaaaaaggta gaaatgttat tacgtaaatg aaaactagag gtgtaaatat
     4381 ggcgaatttg gtttatagtc ctaaagacat tttacaacaa gaattcaaaa caaaaatgcg
     4441 cggctatgat cccgttgaag ttgatgaatt cttagacaat atcatcaaag attatgaaac
     4501 ttacagcaaa gaactccttg ctttacaaga agaaaacgat cgattgagtg caaaagtagc
     4561 tcaattatct aaaactcaag gagcagctca aacacgcgtg caacaaacag aggcaccaaa
     4621 gagcgcagca gtgaccaatt ttgatatctt aaaacgcttg tcaaatcttg aacgtgaagt
     4681 atttggtaaa aagcttgatc aacaagcaag tgcagtcaaa ccagcacaac ctaatccaaa
     4741 taactacaca aatgcagaca caagcttaga tgataatgaa aaaacacgtc aattttaatt
     4801 tttgattaat gaatttgcgg ttttcgggta atcgcggtct gacttgatca ggctgaggaa
     4861 agtccatgct cgcacaggct gtgatgcctg tagtgttcgt gcttagcgaa accataagct
     4921 aaggtactct ttggagtaac ggcaggaaaa caagctaagg cttcggctat gcttaagtat
     4981 ccttgaaagt gccacagtga cgaagcggct ggggaaaccc atccggtgga acgcggtaaa
     5041 cccctcgagc gagcaaccca aacaatggta ggggcgcttt ttgtctggaa gaagaacagg
     5101 acaaaaaggc agtataactg cagatagatg gttaccgctt tgttttttct ccctggaaaa
     5161 gcaaagtaca gaacatggct tatagaaaac cgcaaacatc agggaattag gtcgtgacaa
     5221 aagtaatgcg ttataagaaa caagaaaaac atttgaaagc tatttttcaa tacttcagat
     5281 gaatcgttct tattacaaaa caaacttttg tatcaccggt tattcggaat ttgagtaaaa
     5341 gtgtggggta ggactgatgt cccacctcct ttttttataa ataaggagat tgtgtatgga
     5401 aaaaaacagg aagttcaacc tcgtcgcaac tgcggcaagc gggctagaag cactggttgg
     5461 taaagaatta agagatcttg gaatcgaatg ccaagttgag aacggacgtg cacgttttga
     5521 aggaacaatg gaaacgatcg ctacagctaa tttatggtta cgtacagcag atcgagtaaa
     5581 aatcgtggtt ggggaattcg atgcgtatac gtttgatgaa ttgtttgaaa acgtaaaagc
     5641 attgccttgg gaagattttc ttccactaga tgcagctttt cctgtagctg gaaaatcgat
     5701 caagtcgaaa ctatatagca cacctgattg ccaagcgatc acaaaaaaag caatcgttga
     5761 acgtctccgt acctattatc accgtccagc ttctgtacca ctgacggaaa ctggcgcaca
     5821 ttttcagttg gaagtagctt tgctgaaaga ccatgtgatg gtaactctgg atacgacagg
     5881 accaagttta ttcaaacgtg gttatcgctt agaaaaaggt ggagcaccat tgaaagaaaa
     5941 catggcagca gcacttatta tgttgacaaa ttggcgaaaa gaccgtccat tttatgatcc
     6001 tgtatgtggt tcaggtacgc tttgtattga agcagcctta ataggccata atatcgcacc
     6061 aggattcaat cgtgagtttg cttgcgaatc ttgggattgg ttcagtcaag aaatcatgga
     6121 aaatgttcga tcagcagccg aagaaaaagc agattatgat attgaattag atattactgg
     6181 ttcagatatc aatggacgga tgattgaaat tgccaaagcc aatgcagaag aaatcggatt
     6241 aggctcttcg attaccttta agcaacaagc agtcaaagac ttcaaaacgg ataaagaata
     6301 cggggtcatc gtggctaatc cgccttacgg agaacgtcta ggagaagaag aaactgttcg
     6361 gaaattatat aaagaaatgg gcgatgtatt ccgtccatta aaaacatgga gtaaatatat
     6421 cttgactagc gacttagcgt ttgaggaatt ttacggacaa aaagcaacca aaaaaagaaa
     6481 attgtacaat ggtgcattgc gtacggatct attccagtat tggggaactc gtccgcctag
     6541 aaaaaaagag aaagacgaat aatcttacta attgattcat tcaattggat gtttggagga
     6601 atagggaatg aaagaaaaag agtttttaca agaagtaaaa gaaatcaatc ttctacaaca
     6661 agctttagga atcctcgatt gggatacaca aacaggtatg cctgaaaaag ctagtgcata
     6721 ccgaagtgaa gtagacagct atctttacag catttatttt tcaaaaaaaa tcggtccaaa
     6781 aatcaaagaa gccattcgct atttttctga acatccggaa gaattgtcgg aagttggaac
     6841 tgccgttttt gccaaagtaa aagaagagta tgatttggaa catcgcgtac cggaagatct
     6901 tatgagaaaa tatacagctg ccgtttcttc agcacacgga caatggcaaa aagctagaga
     6961 aacacaacaa ttttctgatt ttcaacacgc attgaatcaa aatattgaat taacaaaaca
     7021 gttgatacct tactggagaa aagatgaaaa gacgaattat gatgttttgc tgaatcaata
     7081 cgaacccggg atgactgtcg aaatccttga taaggtattt gatcagctta aagaaggaat
     7141 tctttcgatc cgccgtacgc tccaagaaaa agggactgaa cctgaaacag actttttaaa
     7201 tcggagaatg acgaaaaaac aacagaagag atttgtagtg aaagtaatcg aacaacttgg
     7261 ttatgatttt agtcgtggtc gtttagatga cactgtccac ccatttatga ctggaattaa
     7321 tccgaatgat gcacgaatca cgacacgatg gaacgaaaca gatttcaaaa tggctgtttt
     7381 tggagtgatc cacgaggctg gtcatggtat gtatgaacaa gacatcgatg ataaatatgc
     7441 gtatacaccg gtttatgaag gtacatcaat gggtatccat gaatcacaat cgttgtttaa
     7501 cgagatcatt attggaagta gtcgcagctt ctggaaaaag caatatcctt tctttcaaga
     7561 atgtgcagaa ggaacattcg atgatattcc ttttgacaaa ttttatcgtt cattgaacca
     7621 tacaaaatca agtttggttc gtatcgaggc agacagtttg acttaccctc tccatatcat
     7681 cattcgttat gaaatcgaaa aaatgatttt caatgaaggg gtagatgttg ctgaactacc
     7741 tgaaatatgg aatcaaaaat atgaagagta tctgggaata cgaccaacaa atgatgtgga
     7801 aggcatccta caagacgttc attggtcagg tgcaagcttt ggttattttc cctcttatgc
     7861 attagggtac atgtatgcgg cacaattact tcattctttg cagcaagaaa tagatgtgga
     7921 aaatgttcta gcgtcagacg attattctcc aatcaaaaac tggatgtcag agcatatcca
     7981 tcaatatggt gcatctagaa aaccaaatca attaatcatg gatgcaacac atgaaccgct
     8041 taatccgcaa tacctgatcg actatatgaa agacctgtat tttaatgtat atcaggtaaa
     8101 ataaaataca atttacaaag atagctgaaa ttcatctaat ctatagatgt tttcagctat
     8161 ttacttaaga aagaagtgga acgaatggat aaattacaag tgatcgctga ttatagtatg
     8221 gaccaattag cgcatgatca aacaggacat gggagcgatc ataccaaaag agttgtcaag
     8281 ttagctgaaa aaattttgga gacggaaccg caggcagacc gctttgtgac gcttgcagca
     8341 gcttacctac atgatacgat tgatgataaa gtcgttaaaa atgagaacga ggcaaagcaa
     8401 cagcttcgtg tattccttcg ctctttgcca atagtagagg aacagatctc gatgattttt
     8461 tccattatcg aaaatatgtc cttttctaaa aatctctctg aagcggccga actatctctg
     8521 gaaggtaaga tcgttcaaga cgctgatcgt atcgaagcat taggagcaat cggcattttg
     8581 cgtactgctt atttcggcgg tggacatggg cacccgattt ttgatcctga actgtatccg
     8641 caaacattca aggacaaaga aaattaccga aaaggcacga cagtgatcaa tcatttttat
     8701 gaaaaactgt tcttgctgtc tgataaaatg aatactgatt acggatatga agaagccaaa
     8761 cgaagagaaa ccttcatgcg tgaattctta gaagaatttt ttctggaatg gtctattgac
     8821 gatgaaaatt ttactccaga caattggatc aaaaataaca aaaaatgatg gaggttgtga
     8881 cagaagtggt cagctccaag atatttcgga tcattttccg taaaagttac ttctggaaca
     8941 ctgtttacca agaggtcgtg agcctgccgt tcagctccga gtcacaagga acaatttcaa
     9001 aaaatagctt ctcgtgtttg atgaatattg taacttgtga ttcggaattg gcaggagaac
     9061 accgtttatt
//
LOCUS       DADVXU010000103         8977 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.107, whole genome shotgun sequence.
ACCESSION   DADVXU010000103 DADVXU010000000
VERSION     DADVXU010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.107"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            12..85
                     /locus_tag="KB953_002404"
     tRNA            12..85
                     /locus_tag="KB953_002404"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46..48,aa:Ile,seq:cat)
     gene            complement(470..967)
                     /locus_tag="KB953_002405"
     CDS             complement(470..967)
                     /locus_tag="KB953_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBB6867340.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(1007..1816)
                     /locus_tag="KB953_002406"
     CDS             complement(1007..1816)
                     /locus_tag="KB953_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBB6867341.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            2150..3007
                     /locus_tag="KB953_002407"
     CDS             2150..3007
                     /locus_tag="KB953_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBB6867342.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            3000..3902
                     /locus_tag="KB953_002408"
     CDS             3000..3902
                     /locus_tag="KB953_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBB6867343.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            3935..4285
                     /locus_tag="KB953_002409"
     CDS             3935..4285
                     /locus_tag="KB953_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBB6867344.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            4334..5257
                     /locus_tag="KB953_002410"
     CDS             4334..5257
                     /locus_tag="KB953_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBB6867345.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            5335..6228
                     /locus_tag="KB953_002411"
     CDS             5335..6228
                     /locus_tag="KB953_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBB6867346.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            6266..6829
                     /gene="amaP"
                     /locus_tag="KB953_002412"
     CDS             6266..6829
                     /gene="amaP"
                     /locus_tag="KB953_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HBB6867347.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            6843..7031
                     /locus_tag="KB953_002413"
     CDS             6843..7031
                     /locus_tag="KB953_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HBB6867348.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            7044..7946
                     /locus_tag="KB953_002414"
     CDS             7044..7946
                     /locus_tag="KB953_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBB6867349.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            7978..8220
                     /locus_tag="KB953_002415"
     CDS             7978..8220
                     /locus_tag="KB953_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBB6867350.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(8476..8847)
                     /locus_tag="KB953_002416"
     CDS             complement(8476..8847)
                     /locus_tag="KB953_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867351.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         3121 a         1553 c         1741 g         2562 t
ORIGIN      
        1 ttatttatga tgtaccttta gctcagttgg ttagagcaga cggctcataa ccgtccggtc
       61 gtaggttcga gtcctacaag gtacataaga ggttgtgaaa aagctgccct gcatcaagta
      121 ataagaacag ccaagtaaaa acagcttctt atgtttttac ttggctgttc ttattactgc
      181 agatgcaagc ttttgaatac cgtttaagga agaggttgtg aaatcagcgt tttgacctga
      241 gtattagctt aacaaacgga aaaatagttc ctcatatttt gccgtttgtt aagctaatac
      301 cgagggtcag ctgattgaac accatttaag aaagaggtcg tgagcctgcc gtttcactct
      361 gtgtcataaa aaagccaatc tactttgata gaaaaagcac gtaggactaa aacagttctg
      421 cgtgcttttt ttattgtgtg ttaaatatcg taaatgaaga tagatatttt tatgattcga
      481 cgatagagac gttgaaatct ttcaacagct ctaaagcgga ttctccgtgt tttctactgg
      541 agactaacat tggatcaaca aaaaagtctg cttcaggaaa agcactctgc aggcctatca
      601 gattagagag tacacacaag tttgtttcaa tcccgactac ttcgatctct ttttgttctt
      661 ctttgctttc aaataaagtt tcctggattt cagctaaagt ttcaggtgga atcgtaaagt
      721 aatacttttt aatcactcgt tcattttctt gtggagaaag aagagggatc aattgcaaat
      781 cttctcgttc ttcttctttg tccttcagtt caactggtat atcccgtgtg aagagaatat
      841 attcattatt ttggcgtgct ttagctaatc tttgagcaat acgttctgcc aattgatctg
      901 actctggaat ataaaattcg ctgtttggat caagaatatg attttgcata tcaataataa
      961 ctaacatttt ctttcctcct acattcatat cgtcatttta ttctttttaa tagaagtttt
     1021 ttaaaatata agcaacacca gcttcttcat tcgtcagtgt tacttggttt gcaatttctt
     1081 ttacttgtcg ctgtgcattc cccatcgcaa tactcaaccc gctgtaattc aacatagcaa
     1141 tgtcgttttc ttgatcgcca aaagccatga tgtcactcga ttcgattcca aggtaatccg
     1201 ccagaaaaac aagtgaatga gctttagtga tacctgtagg cgtgatttct aagaaatgcg
     1261 gatcagagaa tactgattgg ataccatctg cttgcagatc tctttgtagc ttctgtagtt
     1321 tttcttcact cttatctgtt aatgctaatt taagatagct aagagaagga tcttccaaca
     1381 tatttaaaat ttgatcaaaa gaatggttct gtaaattgaa atgttcataa taagggtcat
     1441 ctttgactag atttgattca tcgaatgtca gctgatcatc ttgggtttct cggctagcaa
     1501 tgatggatat tttcgcttct tgagcataga agaaggcttt tctcagtgta tcgctatcta
     1561 tttttgaacg tctgagaact ttcgccattt ttccatcctt catttcagca atcagtgaac
     1621 cattggaaga aatcaaatag ccaccgattc ctaattcgga aaaaatatct ttagcagaaa
     1681 tgctgttcct tccggtagcg acggttacga ttccgccatt ttcatcaaat ttccgaagtg
     1741 cttggatatt ttcttctggt atctttccat ttcgatcaag caaggtaccg tctaaatcaa
     1801 tcgctgctag tttcatttta tcttcctact ttctttttac atatctattt tcctgttacc
     1861 ttaagattaa atgagaattt aaaaaaatga aactaacttg ctttgtcatt atgaattttt
     1921 tcaaaaaaca ttcttaagaa aaaggtggct atatatagta gtttttggtc aatacctttg
     1981 aataaaaagc catgaaaaac gaatagttag cgcttaccga tgcgttttgt gataaaaaaa
     2041 ttttaaaata ttcaatgaaa acaaagcata tattttgctt taaaaccaaa tgaggaatat
     2101 tctagatgta gaacaggtta gaaaaagatt agaaaaagga gtgattttga tggagaagag
     2161 atacggcgta gttcctgtgg taactgcttc agacgaaaat tatgctcctt acttgagcgt
     2221 gatgatcgca actgcattag aaaattgcaa taaaacaaga cgcataaaat tttatgtgat
     2281 tgacgatggt ctatcagaat atagtaaaga aggattggaa gaaacggtaa ataaatattc
     2341 aagtaatgca agtatccaat tcctgacagt ggaaaaagat atctacgaag acttcctagt
     2401 cagtgaccat atcacaacta ccgcttactt aagaatttct ttaccaaact tattagctaa
     2461 agaggattat aagaaagtcc tttacttaga ttcagatgtg ttagtactag acgatatcgt
     2521 aaaactatat gatgagccgt taaatggaaa aacaatcggc gctattatcg atccaggcca
     2581 agtgaaagct ttggaaagat taggaatcga ttccgatgac ttgtacttca attcaggagt
     2641 gatggtgatt gacattgatc aatggaataa aaaagaaatt acagaaaaaa ctatccatta
     2701 cctcagtgaa aatggcgatc gaatcatcta tcacgaccaa gatgctttga atgctgtact
     2761 ttatgaagat tgggaacaac ttcatccaaa atggaacatg cagacttcgc tgatttttga
     2821 acgtcatcca gcaccaaatg aaaaatatga acgtcagtat aaagaaggaa atgaaaaacc
     2881 ttcgatcgtt cactttacag gacatgataa accttggaat acgttgaaag atcatccgta
     2941 taccaattta tacttaaaaa aattagcaca cagtactcta acgaaagtag gcgagatcaa
     3001 tgaataaaaa agaaatagca gtagtcgcaa gttgtaacac aaaattcgta ccacatttag
     3061 ctgcattatt tgtctcagta ttagataatt gcaacccgtc taaatttgtt cggttctatg
     3121 tgattgacga tgatatcgat tttgaaagta aacagctttt acgcttctca gtgaaaaacg
     3181 cacgaatgaa ctcagatgtg gaattcttga aaatcaataa agaattcttt acaaatgtgg
     3241 taatcagcga tcgtattcca gaaacagctt attatcgtat tgcgattccg gaattgttcc
     3301 gcggcacaga agttgaacgt atcttatata tggattgcga catgatcgct ttacaagata
     3361 tctctaaatt atggcgtctt gacttcggtg attcaatcgt agcagctgtt gaagacgctg
     3421 ggttccatca acgactggaa aaaatgaaaa tttcagctaa atcaatgcgt tatttcaact
     3481 caggtttgat gctgatcaat gtgaaaaaat ggttagatga aaatattacg caaaaagtat
     3541 tagatttcat cgaacataat ccagaaaaat tacgattcca tgaccaagat gcattaaatg
     3601 ctatcttaca tgaccgttgg ttaccacttc atccgcgttg gaatgcacaa ggctatatca
     3661 tggcaaaagc aaaaaaacat ccaacagcag caggtgaacg tgaatacgaa gaaactcgca
     3721 acaatcctta cattatccat ttttcaggac atgtgaaacc gtggagcaag gattttgaag
     3781 ggccaacgaa gaaatattac gaaaaatatg cagggatgac ggctttccgt tgtgtagcta
     3841 aattccctaa atatccaaaa tatgcaaaag ttcaaccaaa aacagatgta gttgttaaat
     3901 aatagaagac cacaactgaa gagggggagt aggaatggaa attacaatgt ctaaaaatgc
     3961 agtagagacg ttaattgaaa aggttgggga aaatacaaaa atcgcactcg cgcttatcaa
     4021 tgattccgat ccatttctaa gggacaaagg tgcttttgca aaaggcagct tttttcaaat
     4081 cattcctttt gtttcggaat ttggagaata tgcgactaaa atcgagcatc cattattgga
     4141 catctataca tctaaattag agcaaaatta tttcggtaaa cgattgaaca tggacttcaa
     4201 caaacagctt gacagctttt cattagaaaa tgaaatcgca gtactcgatt ataatatcaa
     4261 attaaagaac tgcttcttca gctaatagtt tcgagaaagt ccaaaaagga ggtatacatt
     4321 gagatactac tttgtggatg aagaaaagaa tgtaatagaa acagatgaaa aaaaatataa
     4381 ttggctcgta cttgatagtg caaagcccga agagattgag caagcaatcg aagaattcga
     4441 gcttcctgat gatattttcg taggaaccga atatccggaa gaagtttctc ggatggagcg
     4501 cctttatgag acgaaactag agcatccatt ttctttggta gtgattaatc tagatcagtc
     4561 agataatcgt atcgaaaaaa aattaacgcc aatatctttt gtactttccg ataatctctt
     4621 gattacttgt atcgatagaa aaaccgaatt tgtggatcgg ctgctgacaa agcatggaaa
     4681 tcatttaaac tcgtttgaaa agatcatcac gtatacatta ttagatattt acacccatta
     4741 cgtgaaagaa ctaagagaga tgaaaaagcg aattgacgct ttggataaag cagctcgtaa
     4801 aacaacggaa aatgaagaac tgtataaaca ggcagatctt gaaagagaca tcgtatatat
     4861 cgatcatacc ttaagagatc aacgggaaac aatggatcaa ttatggaatt ctgaaacttt
     4921 catcaaacgt ttagacgatg aacagctact ctatgatgtc catttgagac agcgacaaac
     4981 agaaaaaatg atcgagatct atcgagattt attagagagt attggtgatc tattcaacgg
     5041 gatgatggac aataatctaa atcatatcat gaaatatcta gattcagctg cattaatcat
     5101 ttccgttccg gcgttgttcg cagggatttg gggaatgaat acaggaggat taccgggtaa
     5161 aagttctgct ctaggcttta tactcgtagt ctttggctct ttgatagcag cagtagtttt
     5221 aggttaccat ttataccgga aagattatac aaattaaaga atctatttaa aacaggtatc
     5281 aaacaaagaa atttgttttg gcaatacaat ttaaaaacta ggaggaatac agcaatgaca
     5341 gaaccaaaat taatcgaccc gcgaaacttg taccatacgg agaaatttcc taaacaagat
     5401 caggatacac cagctttaca gcagaatatg attcctgttc ctgattgtgg agaagaatcc
     5461 tatgaaggca ataatcaact agaaaaccgc cgtgtactaa tcactggtgg agattcaggg
     5521 atcggtcgtg cagcagcgat tgcatttgct agagaagggg cagatatcgc tcttcaattc
     5581 ttcccaggag aagaaaaaga tgcagaggaa gtagcaaaat acgtgaaaga tgcaggacgt
     5641 aagattgtct tactaccttt tgatcttcgt gacgaacaag cacctaaaga aatcatagat
     5701 aaagccgtga gtgaacttgg cgggctagat acgatggtac taaacgcagg acagcaaatt
     5761 tcttgtcagt ctattgctga gttaccactt gagcaagtca aagacacatt tatgataaac
     5821 atcatcgcta tgtttgcttt agtgaaagaa gctgttccgc atatcccagc aggaggagca
     5881 atcgtaacaa caacttctgt tcaagcattc aatccaagcg aacatttatt agattacgca
     5941 gcaacaaaag cttcaatcgc taactttacg atcggtttag caaaacagct tgcacctaaa
     6001 ggaatccgtg taaatggtgt agcacctggt ccaatttgga caccattaca actagacgaa
     6061 ggacaacctt ctgattcgat ccctgaattt ggtcagcaaa gtttactaga acgtgcaggt
     6121 cagccaggag aattagcacc agtttatgtt ttcttagcat cgaataaagc aagttatgtt
     6181 acagcccaaa tttacggagt aactggtgga gaagcgatca acttataaga agttgatcgt
     6241 ttcaccccta tgaggaggtg cgaaaatgaa taaaggtgta aaaacgatcg gagtgatcgg
     6301 aacattactt ttgctatctg tgcttttgtt tgcgatacta ttaagtgcaa cctatccatt
     6361 gccttttcag ctagagaagt ttcgattctt ttccatcact aattcatacg tgcaacaata
     6421 tgtattttgg cttgccgttg catttgctat tttgacaatt atcacgttac ttgttttcct
     6481 gttttatcca aaaacaatcg gaaactttct actgaaacat ggtgatgggg agttgacgtt
     6541 ggataaaaaa gcaattgaag gaatggttcg ttctcacttg catgaagatg aattcatcca
     6601 ttcaccaaaa gtaaatgtca aagcaacgaa aaacaagatc gccgtcaatg taaaagggga
     6661 gttgaaacga acttcagcac tcattggtaa aacaggaaca ttgatgaaag aaatcgaaaa
     6721 tgaagtacaa cacatattgg gaacaaaaga gccagtgaaa gtatcggtga aatacacagg
     6781 ctttgaagaa ccagctaaac gaagcaatga ccgcaaacga gtagaatagg gggggaccaa
     6841 aaatgaaaga actattttcg acatacaagc tccctatcat ctttggggta cttggattga
     6901 tattagccgt tttatggatc accgtgggct ttttcaagac cttattgatc ttgatcatgg
     6961 cagcgatcgg cgtcgctatc ggtttttatc tgaaacaaac gaaaatctta gatcactatt
     7021 ttaaatctta ggaggaaaca tccatgaata acaataacaa aaataataac aataaaatga
     7081 acaaccaaaa accagtgtct gcacatccag acccaacgac accaaaccaa tcaaatggtg
     7141 tgaacgatga aaagaacaaa acagcaccag tatctgcaca tccagatcca gtagtcccta
     7201 acgttcaaca aggagtagaa gagaagaaac atgatgcaga aaagcatgca cctcatcatc
     7261 caggaactcc agaatctaaa gttccagaac aacatgatgc aatcgatcct tctaaacctg
     7321 tggaagagca aaaacatcct tctgaccatg cgaaagcaca agaaaaactt agttctgacc
     7381 acacaaacgg acaaggaaaa cttggttctg atcatacaaa taaccaagga aagcatgatt
     7441 ctgaacagga agattcaatg cgcggtgagc tgaattatga agataaagtt gtgcaaaaaa
     7501 tcatcggaat cgcaattgaa caagtagacg gtttattatc tgcaaacggt ggtttcttct
     7561 ctaatgtggc aggaaaactt gtgaatacag ataatgtaac ggctgggatc gaaacagaag
     7621 tcggtaaaaa acaagtagca gttgatatgg atgtcattgt ggaatatggc aaagatatcg
     7681 aaaaaatctt tgaagaaatg caagaagtaa tcggcaaaga agtaaaaaat atgacacatt
     7741 tagaagtgat cgaagtcaac gctaacgtag tggacatcaa aacaaaagaa gaatttgaaa
     7801 aagatcaaga aacggtgcaa gacaaagtaa ctgacgcagc gaaaaaaacc ggtgaatttg
     7861 cttcgaatca aacagataaa gtaaaatcag cagctggaag cggcgcacaa aaagtaaaag
     7921 aaaatacaga accacgggtt cagtaataag gaagtaaaac attaggagga acgaaacatg
     7981 ggatttattt ggtcacttat cgtcggagga attatcggag caattgcagg agcaatcacg
     8041 aataaaggtt cttcgatggg gattattgca aatgtcgtag caggtttagt tggatcagca
     8101 cttggtcaag cattattagg ttcttgggga ccaagtattg caggaatggc aatcgtgcca
     8161 tcattgattg gtgccatcat tttagtacta atcgtatcat tctttgctcg aaaactgtaa
     8221 aaagactttt aaaaagaggt cgtgaaaaag ctgttcttca tcaagtaata agaacagcca
     8281 aacaaaaata gcttctcata tttttcattt ggttgggcat attatcgaag atgcaagctt
     8341 ttgaacactg tttatacgga ataagaggct gtgacaagac ttttgtcaca gcctcttatt
     8401 tatgcgggca aattcccaat tagattttta aaagtttttc cttaaatgtg tctttattga
     8461 tacagagaag cttctttaat aaaacaatgc ctccctaaat tttcttgaga taggaaaggt
     8521 gaacatacaa aataatacga ccactttgaa aacccagatg atccacaaat ggatcgtatg
     8581 atactgatga agtaaaaagc aatggatcag aaactcaaag agattgaaaa agacaaggaa
     8641 gaaataatat ctctttttta ctcttgtctg ttttagccaa taatagctcg taaagtaatc
     8701 tagacaaaca aatagaagta aacaaagttt cgtaagagca gcaatcgtat catgttgctc
     8761 tgctagacga ttgacgagca ggaaaaatgt tggtaatgga atatagatca cgagtaatga
     8821 aagaaaaaag taaacaataa atggcatact attcctccta attgtgtgag ttcgccctca
     8881 ttttaaaata acgatatttg tttgtgaagc tttttcgtgt cgtaacaaat gttatctaat
     8941 gtatttttaa ataaataatc attattatca aaataat
//
LOCUS       DADVXU010000104         8850 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.108, whole genome shotgun sequence.
ACCESSION   DADVXU010000104 DADVXU010000000
VERSION     DADVXU010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8850
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.108"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            63..1277
                     /locus_tag="KB953_002417"
                     /pseudo
     CDS             63..1277
                     /locus_tag="KB953_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1456..1785)
                     /locus_tag="KB953_002418"
     CDS             complement(1456..1785)
                     /locus_tag="KB953_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBB6867352.1"
                     /translation="MNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSKYH
                     TKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQRL
                     EERMEEL"
     gene            complement(1775..2047)
                     /locus_tag="KB953_002419"
     CDS             complement(1775..2047)
                     /locus_tag="KB953_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HBB6867353.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2498..3301)
                     /locus_tag="KB953_002420"
     CDS             complement(2498..3301)
                     /locus_tag="KB953_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBB6867354.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3279..4403)
                     /locus_tag="KB953_002421"
     CDS             complement(3279..4403)
                     /locus_tag="KB953_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6867355.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4419..5234)
                     /locus_tag="KB953_002422"
     CDS             complement(4419..5234)
                     /locus_tag="KB953_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBB6867356.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5224..6096)
                     /locus_tag="KB953_002423"
     CDS             complement(5224..6096)
                     /locus_tag="KB953_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBB6867357.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6181..7470)
                     /locus_tag="KB953_002424"
     CDS             complement(6181..7470)
                     /locus_tag="KB953_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBB6867358.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7698..8342)
                     /locus_tag="KB953_002425"
                     /pseudo
     CDS             complement(7698..8342)
                     /locus_tag="KB953_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
BASE COUNT         2742 a         1883 c         1409 g         2816 t
ORIGIN      
        1 caaaatcaat tctaccttag atgttcttat atcactctat cagtcctatt tgacaaagag
       61 ccaagaaaaa gacgttggtc gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa
      121 gttgtggttc tactgtggta gatggaaacg ggaaagcaat tatagtgaaa aatgggaaga
      181 aggaaacgat tgtccgtttt gaacaataca atcatatgcc tttggttacg cgcctaaaaa
      241 agcaacgcta tacctgtaaa aactgtcgta cccattggac ggctcaaagt tattttgtcc
      301 gactcagcca ttcaatcgca gatcatgttc gatataaaat tgcttcttta ctgactgaaa
      361 aagtatcttt gtcttttatt gcgaaaagtt gtcaggtatc tttgactacc gttactcgta
      421 cattgaaaga gtttaaaagc tatttaccaa agccttctaa gaagattctc cccagagtat
      481 tgatggttga tgaatttcgt tcgcacgctt ccatagaaga taaaatgagc tttatttgcg
      541 cagatggtga aacaggaaac ttaattgctg tcttgcctac gcgtaaatta cctcgattaa
      601 caagctattt cttaggctgc accaatccag aagaagtaga attcttagtc acagacatga
      661 acgccgctta cttccagctt accaaacgtg ttctgccaaa tgcgaaagta gtgattgatc
      721 ggtttcatat tgtcaaacac atgaatcagg cactcaatga gttgcgtatc cgtgaaatga
      781 atgagcttcg taaagcagga cagaaaagtc aggcagaaaa actgaaaaag aactggcgct
      841 ttctgctaaa aaatcgtgca agcatcaacc attatgagta caaaacatgg aaaagtttcc
      901 gagcaccaaa atacccattt ctcactgaag caatgatgat tgatcgattg cttgagtttt
      961 ctgcgcctct aaaggaggcg tatccctttt ttcatgaatt agtcgaagcc tttcgagaca
     1021 aagaccctga cttatttttc tccttattgg cagaacttcc cgaaacgtta gatgacggct
     1081 ttcgggagaa gcttcaaaac cttctgacct atgaagaagg tatcaccagc gcaatgatct
     1141 atccttattc caatggaaaa attgaagcga agaataccca cataaagaca atgaaacgag
     1201 tatcctacgg atttaaatca ttcgagaaca tgagaattag aatctttttg atcaatcaat
     1261 taatcaatgt aagataacaa aaaatctgaa ccagaatgtg gttcattctg acccggattt
     1321 gattttgcac aactcatcag tccttattga caaagagcca aaaatctgag ccagaatgag
     1381 gttcattctg acccagattt gattttgtac aacccatcag tccttattga caaagagccc
     1441 ttctttttag tattattata attcttccat gcgttcttcc agtcgctgta aatcgcttgg
     1501 acttaattcc ccaatttttt tccatttgac ctttttgttg tttttgggta attcgtttag
     1561 agttcctacg tcaatccaag attctttttt tagtcctgcc tgtttccaat cccttatttc
     1621 aacgtatttc agctttattt tatcggattt agtatgatat tttgaagtaa ttgacagaaa
     1681 cgaaattgtg tccagtccat tttttagaat atagacaggc cggcgtttac cgccctctgc
     1741 agctgtgaaa gcaacgtaga cggttgctat atcattagta ttcatcttca ttgaaccatt
     1801 cttcaatttt ttcgtcagtg tctagttttt ggacaggcaa tttttccgct aatctttgaa
     1861 tattatggat tcgtttactt ttttccgaca attcaataga aaatgggatt tttccttgct
     1921 caacaatttg cttataaaaa acggtgacgg ctgttttggg gctcatgcct agttcatcaa
     1981 taatgctttc tgcttcattt ttcaattttt cgtccataga aatttgtaac ttagccattt
     2041 tctccacctc tttctgacaa acacattata tcacggaaag agttcaaatg gaattaatac
     2101 aactactttt gaactccttg aaatgcagtg tatttatcta aatataagga aaggatatag
     2161 tgctaaatgg ccttgataga tagaaaagtt tcaaaatccg ccacagcgat aattgaagcg
     2221 aacactcaac gacacacaga acaggaaaag actcctaaaa gaaccaaatt aactaggacg
     2281 tgtctgaaaa ctccacaaag atgtggtatg ctctggtaaa aaacgaatgg cacaaccacg
     2341 aagaccgata tggttcttgg aaaactttct ccaccctttt tgtaattgga gggatagtgg
     2401 tttattgata gcgattttcc aagttcttca agtggaacca gacattgagc accacggcta
     2461 aaaagtgaat gaagtcttta tttttaatca ataattttta atgattacca gtaattggtt
     2521 ttttcatgcg ccctatttct atttgatgtg gagttaaata atgctgataa gtaaaaattt
     2581 ttccgtcaat taaactgcca ctagtgaatc ctgttctatt ggtaaaaaga gaatagtctg
     2641 ggtattcatc tacaccaaat gataaagatt cagccataaa ctgtttttta ccagcaaatt
     2701 cagcactgct accttgatgg atatgtccta caaaaatacc taaaatatca ctttcggcaa
     2761 tcaattcagc aaaaccttcc ggaacttcag ttacaataga tggatcttcc cacgacaatg
     2821 gatgatgaac caataaaatc gttccctttt cagcaggtgt acccaaccac tcttgtaatt
     2881 gaatcatctg tcctatcgat atttttcctt cgtgctgggt atcttgagca gaatccaagg
     2941 aaataattcg taacccattt tgattcgtac aatggatata ctctcccgtt tcattgcttt
     3001 caacactcat tccttcaaag aaaacactgc gacgatcatg attcccacga caaaaatagt
     3061 aaggtgtggt tgggaaatat tgatccaaca ttttccgtaa gacttgataa tcagaaatcc
     3121 ccccttcatg tactaaatca cctgttaata gaataaaatc gtacactgtt aacgagagtt
     3181 cttgtaacaa tttttccaat gccaactgtg gatttttaat tgttggaaat agttcagcag
     3241 taaacaagtt agtttgatag ttttctctaa aatggatatc agagagatgc aaaaagcgca
     3301 tggctattcc ctctttcttc aataaaataa tcaggatatc ctaaattttc ttgcgtctga
     3361 taatcaaaaa aatgcattct ttcaggttct atgcggagat tgactgcatc accaatctgc
     3421 caagtaggtt tttcattaag gacaatcatt tctataccat cgactttaac ggtgtacgca
     3481 taactttgac ctaatagctc acgatacttc acttgaccgt gtaaatcgct tgtttctttt
     3541 gaaaaggtta cgtgttctgg tcttatgcct aaatacaatg agccactaac ctgaagaagt
     3601 tgtttttgcc aagctggatt caatgttaaa aattgactgg ctacctttaa tgttccttga
     3661 tcataagtaa ctggcataat attcatgcct ggcgaaccaa taaaatttgc agtaaaagta
     3721 tttttaggtt tcgtataaac atttttcggc gtatcaatca tctgaattct accttcattc
     3781 aataaaacaa tccggtcagc taacgtcatc gcttcaattt gatcatgagt gacgtagaca
     3841 aaagtttgtt tataacgttg atgaattttt accaattctt ttcgtgcatc caatcgtaaa
     3901 cgcacatcca aatttgataa cggttcatct aataaaaaat aattactccg tttaaccacc
     3961 gcacgagcca atgctacacg ctgccgttgc cctcctgaca aatctttagg aagtcgatct
     4021 tggtatggcg ttaaacctaa catcttcaaa gcttcttcca aacggtgctg aatgtcagct
     4081 gtcttaattt tattcgccct taatgcaaaa ataatattct cctttacagt catgtgagga
     4141 tataaagcat aattttgaaa aaccattgcg atctctcgtt ctccacaagg tagcttattt
     4201 gctagttgtg tacctaaaaa aagctctcca ctcgtaattt tttccaaacc agcaatcatt
     4261 ctcagcaaag tagattttcc tgaaccagat ggtcccagta acacaattcg ttctccagcg
     4321 gccacagcta aatcaatttc ttttaaaatg gtatgttctc catatttttt tccgatattt
     4381 cttaattcaa gcgtttctcc catgatttgt cctccaattt atttaatacc tgcagaaaca
     4441 aaagtcccga taatatattt ttgaaaaatt gcatacaaga ctaacggtac aaacattgta
     4501 attaaactaa tcgccattgc tacggtaaaa tttgtcccac cttcagaact aatataggtc
     4561 tgcatcgcca acggcaaagt ataattatct actgtccgta aaattaatac cggccaaaca
     4621 tactcattcc aagaggtaat aaaaaaccaa ataccggtag aagtaatcgc atgtctagta
     4681 agaggaaaaa caattttact catgacggtt gtttgtttga tgttatccaa tgccgccaca
     4741 tctagaaggc tcactggaat ttcccgcatt gtttgcgtca ataaaaaaat gcccatcgca
     4801 tcagcaaatt gtggtattat cacaccccaa gtagtatcaa ttaaattaag ttttgcaatc
     4861 aacaaataat taggaatcat cgcaacagta aaaggaatga acatcgtacc aatgatgata
     4921 aagtaccaga ttttcttccc tttgattcgg caataagtaa aactatatgc cgcaaaaaaa
     4981 gctacgccta acttaacaac cgttataatt gtagcaataa aaaaagtatt tctcgtaatt
     5041 ttgataattg gaagattttc gaacaaatag caaaaatttt ccaaggtaaa cggctgtggt
     5101 atcaaactag taatcgtctg ataggcatct ttcaatggct taaaagaatt ggccataata
     5161 aacaataatg gatagataat aatgattatg atgaacataa ataagagatg tggcaaccag
     5221 cgtttaattt tcatagtaaa cccccttttc cacaaattta aatattaccc ctaagaaaac
     5281 agcaaatatt aataaagtta aaactgaaat tgctgaagat tctccgactt tatacatttc
     5341 aaaagccttt tgataagcat aatagatcac gtttgcacta ccattattcg gtccaccacg
     5401 agtgatgatc ttaatcggag taaacacata ctgcagacct tgaacaatgg tcatcaccaa
     5461 taaatatagt cctgtcgaac tactcatagg caacacaatt gtaaaaaaaa tgcgccagat
     5521 gggtacacga tccaacttag ctgcttcgat catctcttga tcaattccca caattgctgc
     5581 aaaaatgaca atgaaattat aaccaaaaac tttccaagca gtaatacctg caatcacagc
     5641 aattacccaa ccttgtaaat tcatccacat aggcagtctt aaaccaaaat ttcccaacaa
     5701 gacagcaatt ggtcccgata ccggatttaa tatccatgta aaaaccatcc ccgccactac
     5761 caaagaaatc acagctggaa taaaaaataa tggtttatac aaattctgcc ctttttctat
     5821 aactattcct aataaaaagg atgtaatata tgggaacaca cagctcagca agacaaatag
     5881 aacggtatag gacaaagtat tcttcaacac tttatgaaag actggatcag tcagcacctt
     5941 tacatagttg gcaaagccaa caaattcttt agtgggggcc accatattcc agtcaaaaaa
     6001 actcaaatag atcgtataaa tcaatggata aaaaacaaac acgcccaaca aaataattgc
     6061 tggcgccaga tacaaataac ctacatagcg cttcatttcc gacagcctcc tcatccttat
     6121 gtcaaaagca gacgaccttg tttttccttt cttacaggcg ctgtcgtctg cttgctttgc
     6181 ttatttgagt aactcattaa tttccttttg agtagctttc atcgtttcat tgacggaatt
     6241 acctgataaa attttatccc gcatatccaa caatttttgt tcagcttcta atccagcatc
     6301 tccaggaaat gaactccaac tgaccatctt atccatctga tcaattgctg gtatcatcat
     6361 ctgattttca gctaagaaag cttttaatcc attttgggcg tctgcaacat ctttacgtgg
     6421 tggtaaataa ccagtacctt ctgtccattt agctaaagat tcaacactat aaagatattt
     6481 taagaaatcc caagttgctt cctttttctt atccgtttgt gatgtaacag ctaacattgc
     6541 gccaccagca ggtagtttaa cttttttccc ttcccaactt ggtgactcaa ctgctttagc
     6601 agtaaaagta ctattttttt gaatgttgtc cctttgagca atagtggtat agaccatcgc
     6661 gacgttacca tcaataaagc tttgcacacc ttgttcccac gtagtgtgta atgccgtctg
     6721 gtcttttacc atcctttgat ataattcata agcagcgata ccatctttag aagcaaaggt
     6781 agctttgcca tcttttatta cacttgcccc attactttct aaaattgctt gttgtgccca
     6841 attatcagca ggttcttgaa tatacacgcc atactttcca gttttttctt ttattatctt
     6901 agaaaaagtt tgaacttctt cccaagtttt tggaccattt tcatccaagc ccgctgcttt
     6961 taatatatct tgatttaaat ataaaaccgg attactaatg gagtaaggaa tacccacttg
     7021 tttaccatct ttttcagaca gtgccatgat attgtctaaa aaatgatctt taaaataatc
     7081 agcattatcc ggatccactt cttctactaa agattgagga ttagtatatt tgaaattttc
     7141 tgcaaaataa tctttaaaag cccatcccac ttgtactacg tccggtattt ctttggaggc
     7201 aacagaagct tgtaaatttt gcatcaagcc tttatacata tcaggattat attttgctac
     7261 aaccttcact ttgtcttgag attgattgta tttatctact aattcattca ctgtttttcc
     7321 acctagggta tccgcattaa catgccaata ctctatcgtt acacgaccct ccgatttttt
     7381 tgttgtcttg gtattcccac taccacaagc tgccaaagag atgacactta aaacagacac
     7441 aacccctata acaagatttt tctttaacat aacattaact tcctcctaat ttctttatgg
     7501 ccatttttct aatcaagaaa tattatgaac taccaatgta aatttctagt taattttctg
     7561 taaataattc gaaatttgtg catttcgggg tacagggtca ctggacatat ccatcaagct
     7621 aagaataaag cccccatatg gtcatgtatc ccatctccaa atctcttaac tcctctgttt
     7681 tgattataaa atagtttatt ctgtgctcct ttcttccata aataggccca tgcacaacgt
     7741 gaggttatat tataaacacc acagcgcgca aggtactcaa tcgtttctag ttcattgact
     7801 gtccatacag aaaatgtcaa tcctagctgc tgccccactt cacgggccaa cttagttaac
     7861 catccttcat gaatattgac gatttgaacg ctttgatagg ctaatttctg taaggtatcg
     7921 atcaaatctg cttcggtaat atccccaagt tttttccctt cgaactgatt ttcaatatta
     7981 taaaatatat gctttcccca aatcgcagcc caagataatt gtacatagcc actaagaaca
     8041 atttgtggcc agatttgata ggtttgtgcc agcgaaaaaa cggccatttc caatccctct
     8101 tcttttaaat cacagttaat ggtcatggtt gccttctcat tggccactgc ttgaaacacc
     8161 tgctcaaggg ttaccgaatc tgggacaact tcttacctgt catgggctag tattagcgta
     8221 ccatctgttt gctgacgcac atctacttca aaacaaagct ggcgatccaa acaaaagcga
     8281 agaaatgccc agctattctc ctccgtattg tcactaccag agtgtgcagt gatcatggtc
     8341 attttgaccc ctcctttaat tccgtgattt tctcccacac gtccactata ccgatgaagc
     8401 gttaatttct tgttaatttc ttgtaaattc cctgcgtttt ttcagtaaac tagttgtaaa
     8461 tcttgcatgc tccttcctac gaactcatga atcgtcgatt ttgcccttct agctagtttc
     8521 accaattaag tagttctaca ttccccgctt ttaacctact acaccttttt ttgttttcag
     8581 ctattccttc ttcttgctga aagtccttga ggttctttct tacaggatac catcaaggtg
     8641 ttcaatggct tattcccatt gaatgaccga gacttactat cagatttctg ctcgttgaac
     8701 caagtctttg atacaggatt tcttccgtca tcttctcaca aataccacct tgcgtttctc
     8761 tagcgattgg tcgatgtgca ccctcccagt ggactgtcac tacccctttt tgctatgcct
     8821 agcccacaaa aaaagctgtc ctcacagaca
//
LOCUS       DADVXU010000105         8817 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.109, whole genome shotgun sequence.
ACCESSION   DADVXU010000105 DADVXU010000000
VERSION     DADVXU010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.109"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            38..1099
                     /locus_tag="KB953_002426"
     CDS             38..1099
                     /locus_tag="KB953_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBB6867359.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            1102..1998
                     /gene="murQ"
                     /locus_tag="KB953_002427"
     CDS             1102..1998
                     /gene="murQ"
                     /locus_tag="KB953_002427"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBB6867360.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            2012..3994
                     /locus_tag="KB953_002428"
     CDS             2012..3994
                     /locus_tag="KB953_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBB6867361.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(4059..4505)
                     /gene="nrdI"
                     /locus_tag="KB953_002429"
     CDS             complement(4059..4505)
                     /gene="nrdI"
                     /locus_tag="KB953_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBB6867362.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            4716..6140
                     /locus_tag="KB953_002430"
     CDS             4716..6140
                     /locus_tag="KB953_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBB6867363.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(6321..7094)
                     /locus_tag="KB953_002431"
     CDS             complement(6321..7094)
                     /locus_tag="KB953_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBB6867364.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(7091..7996)
                     /locus_tag="KB953_002432"
     CDS             complement(7091..7996)
                     /locus_tag="KB953_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBB6867365.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(8150..8470)
                     /locus_tag="KB953_002433"
     CDS             complement(8150..8470)
                     /locus_tag="KB953_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HBB6867366.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         2777 a         1583 c         1876 g         2581 t
ORIGIN      
        1 atagaaatga agacggatac agaaaaagag ggataatatg tttggctttt caatatttat
       61 gaacgattct ttgtcagaag agaagaaaca gtatatccgc caaatggcat ccaatggctt
      121 tgtcggtatt ttcacttcca tgcatatacc agaagatgat gtttcggcat atcaaaagcg
      181 attgatcgaa ctaggaagcg aagcgaaaaa gaataaacta gaattgatgg tcgatatttc
      241 aggagaggca ttggaacgag cgggtttttc attagaagat ctaacgcctt tgaaagcaat
      301 tggtgtaaca ggtttaagaa tggattatca tatttccaat cagcagattg ccgactggtc
      361 acatcaattg aaaatcagtt tgaatgctag cacgatcacg cctaaagata tcgatgaatt
      421 gaaagaagca gaagctgatt tctcacagat tgaagcttgg cataattatt atccacgtcc
      481 cgaaacaggg ctggacaaag aatggtatca gaaaaaaaat caatggctca aaattcaagg
      541 tctccttgtc caaggttttg taccaggaga tacagagttg agaggcccac tatatcaagg
      601 attgccaact ttggaagaac atcgagggat ccatcctctt gcaggcgctc tagatctatt
      661 agcaagtaac acggatattg tttacatcgg agatgcaggt ctgtcagaaa acgttcagga
      721 gcaatttgct tctttccaaa aagaacagac agtcttgctc catacggaac ctgtagatga
      781 agaattttac gaatatatat tagggaagca tacgaatcgg caagacgatg caagagatgt
      841 catacgaagc gtagatgcac gttttcgaga gattccgcct atcccagcaa gaaatacagc
      901 tacgcgtatg aaagggtcta ttactttaga taacgaaaag tatttgcgct atatgggtga
      961 gatccaatta accaaatatg atttgccagc ggatgaaaaa gtgaatgtag tagctaaagt
     1021 tatcaaagaa gagctaccat tgatcaacca aatcaaagct ggtatgaact atcaatttat
     1081 aagaaaagaa ggaagataaa aatgagcaaa atcaatttag agaacctaac aacggaacgc
     1141 agaaaccaag aaacttttgg attagatgag atgagtattt ctcaagccgt tcaactaatg
     1201 aacaaagaag atcagaaagt ggccgctgcg gttgaaaaag aactgacaag tatcgaacca
     1261 gtgattgcta acacgattga gtcctttaaa aaaggtggac gtctgattta tatgggagcc
     1321 ggaacaagtg gaagactagg tgtgttagat gctgctgaat gcgtaccgac attcggtgta
     1381 gaaccggaaa tggtcatagg tctaattgct ggtggagaag aagcaatgac tgtagctgtc
     1441 gaaggggccg aagattctta tgagctaggg caagaagatc taatcaagct gaatttgaca
     1501 aaaaatgata tagttattgg tatcgcagct agcggtcgta caccatatgt gatcggtgga
     1561 ttagattacg caaaatcgat cggtgctgtc acaggaacga tttcttgcaa taaacaagcc
     1621 gaaatcagta aacacgcgga tttcccaatc gaagtcgatg caggtccaga atttttgaca
     1681 ggctctactc gattgaagtc aggaacagca caaaaactta tcttgaatat gatttctact
     1741 atcagtatga ttggtatagg taaagtattc aataacttga tggtcgatgt caaaccaaca
     1801 aatgaaaaac taatcgaacg ctcaaaacga atcatcatgc aagcgacaga agctgattac
     1861 gaaacagctg aaaagtactt tgaagaagca gatcaaaatg ttaagctggc tatcgtcatg
     1921 attttgacaa atagtgataa agaggaagct caagcaaaat tagctgaagc aaacggattt
     1981 gtaaaaggga ccttatgagg gggaaaagac catggcaaaa aacgaactga ctttggaaga
     2041 actggcaaaa caaatttata aaggggcagg cggcatgggc aatgtagaaa gcattgtcca
     2101 ctgcatgact cgtgtgcgta tgaagatcat caatgatgat cttgtaaatg tctctgaatt
     2161 aaaagcgatt ccaggagtgc ttggtgtagt agaagatgaa cagcttcaag tcatcatcgg
     2221 acctgggaaa gtgaacaaag tagcacagca aatggttgat ttggcaggcg tgaaattagg
     2281 agaaaaactt tcttctgtaa gtacagctac agcaaattca tcagcatctg gaaaagaccg
     2341 tgtaaacgaa cgggcacaag aaatgaaagc ggcacaaaaa tcgaaacaaa aaccatctaa
     2401 aatcaaatcg gtattaaaag acatttctaa tatctttgtg ccaatgattc ctgcatttgt
     2461 cggtactggg atcgtggcag ggattgccgc agttttagca aacttagtca cagccggaac
     2521 acttgatgca gcaacatggc aacaatatat cgatgtgatg aacatcttga aaaacgggat
     2581 gtttagctac ctagttatct atacaggtat caatgctgca caagtttttg gtgcaacacc
     2641 aactcttggt ggagttatcg gggccgttgt gatgctgaca gggatgaatc cggaagcacc
     2701 actgaaaaac ttatttactg gtgaagctct tgcagctgga caaggtggga tcctaggtgt
     2761 catcttcgct gtttggctat tgtcaatcgt tgagaaaaaa ctgcataaag tagtacctga
     2821 tgcggtagat atcatcgtta caccaactct ttcattgatt gccatcggtc tgattgaaat
     2881 tttcttcatc atgcctttag caggtttcat ttctgatggc atggtcggtg ggatcaactg
     2941 ggtacttggc gttggtggag catttgccgg cttcgttcta ggcacattat tcttgccgat
     3001 ggttatgttc ggactgcatc aaattttgac tccaatccat gtgcaaatga ttgatgaaac
     3061 tggtcgtacg ctattgcttc caattcttgc gatggctggt gcaggacaag tgggtgcagc
     3121 attggcatta tggattcgct gcaaaaaaga taaagaatta acagaaatga tcaaaggagc
     3181 tttaccagtc gggatcctag gaatcggcga accattgatt tacggagtaa cattacctct
     3241 aggacgccca tttattactg cttgtatcgg tggaggtatc ggcggggcag tgatcggaat
     3301 gatcggaaat gtcggggcta tcgctattgg accttcagga gccgctttga tcccattgat
     3361 ctctgacggt aaatggttag gctacatcct tggattattg gcagcttatg ctggtggctt
     3421 cgttgctaca ttcttcttcg gtattcctaa agaccgttta gaaaaagaag aattagcgac
     3481 tgaaacagcc acagtaaatg aagtgcctga aaaagtagta cctcaaactt cagctgaaag
     3541 tgtgactatc ctctcatcag ttgctgacgg aacagttgtt tctttagata aagcaagtga
     3601 tccagttttt gcacaaaaga tgatgggtga aggctacttt gtagaaccag aaaacggaca
     3661 tatctattct ccagttactg gggtagtcag ctctgtcttc ccaacgaaac acgctattgg
     3721 gatcacaacg ccaaatggtt tagaaatctt gcttcatatg ggaatcaaca cagttgactt
     3781 aggcggtacg ccatttgatg tgaaagtaac agaagggcag caagtgacac ctgatacgct
     3841 tgtagcagaa gctgacttag aagcgatcaa agcagcaggc aaagaaacat ccatgatcgt
     3901 cttggtaacc aatatggatc gtgtaaaaaa ctttgtcttg gaaaaaacag gtaaagcaaa
     3961 agcaaaagca cctgtgatgg atgtcaaagc ttaatatgat agaaacaata gaaacgaaag
     4021 aagaaactga aatctatttt tcagcttctt cttttttatt atttattgtt ttctgctaaa
     4081 atctgataaa ttcgtgtcac atcagatggt gttccccgta attcataatc tgccaagaaa
     4141 ggaaagccaa atgtttgtgc atattgtttg gctgttagac aatactgatt attgaagttt
     4201 ttatttccgc taccgactac accaagacaa agtttatgat tgtcttcatg ttccagatat
     4261 tcacgcatcg tttcggtcaa gatttctgta tcaccgttat ctaaaccatt tccgccgtct
     4321 aaataagtag gaacgaaagt aaaaaatggt tctgtttctt tcgtaaaatc actattttca
     4381 tggatttctt ttaaagtaac ttcaggattg acaggatctt ctgcatgcat tttttcagca
     4441 tattctgcta gatgtttggc aaaggcacgc gtatttcctg aaatcgatat atataaaatg
     4501 ttcatggatc atcctccaat tctttccttt tagtatagca tcttttttaa gttttttggt
     4561 ggtgtaattt ggaaagggac taggcgcttt gcttgtataa tgagaaaaaa gccggtagaa
     4621 ttatatttct attaaagttt ttattaatta agtaaaaata aaaaaggttt gttatacttt
     4681 ttgataaaag cggttttatt ttggaggatg cttacatgga tacagagcaa cagaaaaaag
     4741 gaattggttt aattccactt gctgccttag taattggttc agcgattgga ggtggcgtat
     4801 ttggtattat gacggacatc tccggatcgg ctggcggccc agctattttc agttgggtac
     4861 tcgtaggaac agcaatgttg atgctctcct tgtcgatcaa taatatcaat aaaaaacgtc
     4921 cagatttaaa aggcgggatc tttagttacg cagaagaagg ttttggccgt ttcgctggtt
     4981 ttatcagcgg ttggggttac tggctcacat gctggttagg caacgtagct tttgcaacat
     5041 tgttgatgag cgcattagga tatttcttcc cagtatttga aggtgggcaa aatctggcat
     5101 cgatcgtgat gagtacagtc tttttatggg gatatgcatg gctagtgaat cggggagtcg
     5161 aaaatgctag tttcgtcaat gctatcgtca cattctgtaa attagtgcca ttatttttat
     5221 ttattgtagt agctatcagc acgttttcga tccaacaatt cattgataac ttttctagcg
     5281 acattatatt gaatgcgaat ggacagccga tcccgttttc cagtcaagta ttgagttgca
     5341 tcatggtgat gctttgggta tttgttggta ttgaaggagc ctctgtcgta ggttcacgag
     5401 caaaaaaaca atcagatgtt gggaaagcaa cgattctagg gatattcggc ttgattatta
     5461 tttacgtctt ggtttccatg cttccatatg ggacaatgac gatgaatcag ctgcaaacga
     5521 tcaaggcgca gcctgctatg ggctatgtgt ttgaaatgat ggttggtaaa tggggagcag
     5581 tcgtgatcaa cggcggtttg atcatttctt tagttggtgt ttggctctca tggacgatct
     5641 taccgattga aacaatggga aacatggctg aagacggact tttgcctaaa gcatggggaa
     5701 aactcaacaa aaatggggca ccaacttatg ctatcgtatt gacgacgtta tgtacgaatg
     5761 tctttttact ttcattatta ttcactgaca gtgcttataa ctttgcttac acacttggta
     5821 cagcagctat tttcttcacg tggcttttct taggattgta ccaaatgaaa ctttcttatc
     5881 agcggaaaga atgggtccaa tttttagtag ggcttttagc aagtgccttt caagtatggg
     5941 ctatgctagt cgtagccttt aatgaagtaa tgcttgttct tgttcttttc ttgccaggca
     6001 tttattttta cctcaaagca cgtaaggagc aaaacgagcc tgtacaacat atccacttag
     6061 ataagatttt acttataggt gtgacgatgg tcggagtgat tgcaatggtt ttatttgcca
     6121 ctgggatgct tcacgtatag aagtataaaa gaagtataaa gagaggttgt gagcctgtcg
     6181 tttattcaaa aacatactag gaaaggagct gtgacacaaa tgttgtcaca gctccttttt
     6241 cagaataaac ggtgtttaag agacagccaa tcggcaataa ggctgaacgt ctctttcaca
     6301 gcctcttttt tgatgagtca tcatacattt tttcgcttaa atacgagata tcctaatgct
     6361 aagaacaaaa cgatgtaagc taaattaccg aatagcattt gattagtaga aagatgagtc
     6421 aattgcttca tgatctcttc gtttccaatt tggttttgca gattcaacat actgatcgga
     6481 ttccatttga tccactccca tttttggatg agtgcaataa ggataccaga tatgattgac
     6541 gaagcaaaat aaaagacgat tcctgctgag atggatgcgc cggaactatt gataaagcaa
     6601 gcaatcatca acactaaact taaaatcagc cataaaccga tataactacc caaagtataa
     6661 gtgaacaagc tttggatcag tgtttgacca gtagaaacct tctccgtgaa ggaaatcgaa
     6721 gggaaaagaa tcagtttcat caacacagtg acaataatag ctaaaaggta taaataaaca
     6781 gaataaataa ctaatgtgat ccatttgctt actaatattt ctcctcgaga atactttcga
     6841 taaaggagat ttttcaatgt accatattgt gcttccatcg aaatgatcgt gcttgcagct
     6901 gcaatcatga taaagacaat ccaagatgtg gcactaaaaa gctgagcagt catttcaact
     6961 ggatcaatga tcgtcgtgta ttttttggct agaagtgctg ttcctatgag caaaacaact
     7021 aatagaacag ataaaacagc agttgacttt ttcttgatca atttaaataa ttcttgtttt
     7081 acaagcaatg tcatttttct tcttccccct ttactgaatc cagaatttcc aatagagaag
     7141 attccaaatc attcgtcaga tgctggacgt ctgtcacatg gatattttgt gcagacaata
     7201 gctggatcac ggttcctaat aattcttcgt tttcttttaa aagcttgatt ttatcaggat
     7261 cttccaaagt atatccagct tctgtcaaga tcgttcgagc agctagatca tttgttgtag
     7321 agataacgat gaaatcttgc gctgtttggt tcaattcagc cattgtacat tgtttgatga
     7381 tctttccttg attgatcagg atcagctcat cgaccatttt ttcaagctcg ctgagtaagt
     7441 gactggagat caagaaagag gtgccctgag tagccaagtc ggtaatgatt tttcgcacat
     7501 ctcttgttgc ttgtggatcc aaaccgttca tcggctcatc taaaataacg atagacggat
     7561 ggttcagtaa ggcaagagca attcccaatt tttgtttcat tcccaacgag taggatttcg
     7621 cttttttgtg aatgtacttt tccatcttga gctgattcac gatagtgtcg atagcagata
     7681 catcgttggt ttcagagaat aacttcagat gatcatagcc tgttaagaat ggatagatac
     7741 ccgggtattc gatcaatgct cctacttgtt ccaacccttt atgactagag ggagaaatct
     7801 cattttcacc aatacagatt gttccatctg agtaatggat cagtcctaaa atagctttca
     7861 tgatcgttgt ttttccagcg ccattaggac ctactaatcc aacgattttg ccaggggata
     7921 agctaaaact gacatcgctg agggcttgtt tgtgaccaaa gttcttggat aattcttgga
     7981 ccgaaagact tttcaaaaat attcactcct tttctataga tagtacaagc atacacctga
     8041 tgagtggaga agtcgctagg tcattggact tatttcttat tttttcataa aaaaattgaa
     8101 tattaaataa aaaggcttat tttttctgat cagtacttat tccaagtgtc tagtggccgc
     8161 tagtaatctt gatgccaatg atactgatga gtagcatccc cacaaagaac caagtggaaa
     8221 gattcagctt atcatgaaac aagaccacac caattatgac cgaaccaact gcgccaatcc
     8281 ctgtccagat aggataagag agactcatcg gcaaactttt gattgccttt gacaaaaagt
     8341 aaaaactagc aatcatgcca atcactgtgt aaaaagaata attgatttta gaaaaaccat
     8401 cactccattt catcatcgtt gaccagaata cttccaaaat tcctgctatg attaattcca
     8461 tccatgccat acttacattc ctccttaact ataaaaaaat acacaaaaag aggaaaatca
     8521 tcccttcaaa gacctaatgg aatgatcatc ctctttatcc tacccggtgg cagattgtat
     8581 atctttttct gtagtatagc acacctatcc agatatcggc aatctcatac tttttagtaa
     8641 agtgtaccaa gcactgacgg atatatcgtt agcggaaaag aagatgaatg ctttgcaatt
     8701 tcatagaaag aagttgttat ttattaatca tttaaaaaaa acagtaacta gaccttattt
     8761 ctatatatta tcgggcaata aattaaaaaa agctttacct tacaataagt aagataa
//
LOCUS       DADVXU010000106         8555 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.110, whole genome shotgun sequence.
ACCESSION   DADVXU010000106 DADVXU010000000
VERSION     DADVXU010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.110"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(132..1085)
                     /gene="menA"
                     /locus_tag="KB953_002434"
     CDS             complement(132..1085)
                     /gene="menA"
                     /locus_tag="KB953_002434"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HBB6867367.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1098..2201)
                     /locus_tag="KB953_002435"
     CDS             complement(1098..2201)
                     /locus_tag="KB953_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBB6867368.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2291..3247)
                     /locus_tag="KB953_002436"
     CDS             complement(2291..3247)
                     /locus_tag="KB953_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HBB6867369.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3528..5462
                     /locus_tag="KB953_002437"
     CDS             3528..5462
                     /locus_tag="KB953_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBB6867370.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5919..6335
                     /locus_tag="KB953_002438"
     CDS             5919..6335
                     /locus_tag="KB953_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBB6867371.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            6349..6915
                     /locus_tag="KB953_002439"
     CDS             6349..6915
                     /locus_tag="KB953_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HBB6867372.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6938..7924
                     /locus_tag="KB953_002440"
     CDS             6938..7924
                     /locus_tag="KB953_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBB6867373.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(8079..>8555)
                     /gene="scm"
                     /locus_tag="KB953_002441"
     CDS             complement(8079..>8555)
                     /gene="scm"
                     /locus_tag="KB953_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HBB6867374.1"
                     /translation="STDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSST
                     TTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNR
                     TNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         2516 a         1650 c         1739 g         2650 t
ORIGIN      
        1 tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat
       61 aaggagctat ttttatttgg ctgttcttat tacttgatgc agggcagttt tttcacaacc
      121 tcttcatgct gttaaatggc ttgcataatg attgttagta atagccccaa tacataactg
      181 gcattgaata agaccatatt tttgatggaa tagctgaaac tgataggctg gggtaacgtt
      241 tgcttgaatt gatccaaatg cttcttcaca attggcaagg tcaagaaggt gaccaagatc
      301 ggccattcat agataccgct caacaacccc attaaaatca cgaaatagca agcatacatc
      361 aatagctgga ataagactgt tcccgcagga cgtccgatat aaaaaactag tgtatagcga
      421 tgattttcaa tatcacggtc aagatcccgc aaattattag ccaacatgat attggcaatc
      481 gtaaacacca agggtaagga agctaatacg attgctgcta tagcccaaag atttcctacc
      541 aaagcaaatt gtccagatga aaagttgact gtcagatcat agacatctga accgaatgta
      601 ttgacataaa cgaccatggc aaaaattcca aggcccatag tcaccccact gaaaatttcc
      661 cctaaaggca ttcgagataa aggaatcggt ccgaatgtat agaatactcc aataaaacaa
      721 cagataaatc ccatcaaaag taaaagccaa cccgtattga tcgttagaat aacgccgata
      781 acaaaaacaa atgcaatcat agctaaaatc atgtttcgaa tcaatacagg agatagattt
      841 ctgcggccaa tgacattttc ttcatactta tatacttcag aatgagcctt ttcataatcc
      901 atgtaattat tgatagcggt agtcgctaaa tcaaaaacta gcattccaat aaaaaataaa
      961 aaagtgttta gccaatgtat ctcatgaaaa aaagcgatag agaataatac ccctaccgca
     1021 aaaggaaata cactagccac ctttgttcgt atttcaacta cttctaaaaa gatttttagt
     1081 gtcatggttc gtactcctta ttttagttca gaacggtcac ccatcgtgta atgggagtcc
     1141 tccgataatt tgaaggtgtc tttgattgga tcggtcacat atactttatc gtcttttgtc
     1201 acgaagatcg cttccgtccc gttattcaat tcgctttcga cgtattccag tccttttttc
     1261 acacccattg aaaagactgc tgtagacaac ccgtcaccat caattgattt atctgttatg
     1321 atcgtgacac ttgcgatatc attgtcgaac ggataacccg tttttggatc aaacaaatga
     1381 tggtattttt tgccatctac ttctaaataa cgctcgtaaa ttccagaggt aactaatgtt
     1441 ttgttccgtt ctttaataga acctaaaaca gatccgcgag ccatgttcgg atcttggatg
     1501 ccgactgtcc agtcttggtt ttcgcctctt gggctgtggc ctaagacata gacattaccg
     1561 cccaagtcaa cgatggcagt ggtcactcct tgttttttca agactttcac tacttcgtcc
     1621 gtaatgtaac ctttggcgat tgcacctaaa tcgatgatca ttcctttttc ttttagataa
     1681 acagtttttt ctttgtcatt tagttcgatc ttatgataat ccactaattt caatgcctga
     1741 tcgatttctt cttgggaagg ttttctggcg tcatcaaaac cgattcgcca taattgtgtg
     1801 attgcaccaa tcgccatatc gaatccgcct tgagaatctt ggctatattc gtaggcccgc
     1861 ttgactaacg tgtagacgtc atcagaaact tttactggtt tgatcccagc ttgctcattc
     1921 acttcatcga tctcagaacc tttttgattg atcgtgattt tatcaccaag ttcttttact
     1981 cgatcgaagg caggtttcaa tgctgattct ttgccttcat catacacacg gatacgaacg
     2041 taagtcccta acaagaactg ttcatcagaa tatggatctt tcaatatctt ggtagctgct
     2101 gaactttcag tggttgtttt atttgctgag ttgctgcagc cagttagcac cataaaagag
     2161 atgcccagtg tgattaagga aaatagtagt tttttccgca tctttttcct ctcttcattt
     2221 tgaaaggtga gacgactgcc taatctggct atcgtcccac cctccaactt ttattagtat
     2281 gattatgtga ttatttttct gttacgatat tatctacttc gattgtttga gtgtcgcctt
     2341 tttcagctgc attcactaat tgttgtgcgt acatgatgaa tgaatgtgag ctgtgtgtag
     2401 cacctgtaac aacttctaca ttagcaggtg agccatcttc ttcgcccatt gctttgacga
     2461 attcgtcgtt cagagtaggg atgtaagttt ttggttctgt accgcttttt gctttcatat
     2521 tttcattata ttctgtatca tcttgttttg atttaccgtc tttgtcgata taatcaaagt
     2581 ttgattcagt tactttgccg ccagcaactg tcatttcgaa ttgcacacgg tagccgttag
     2641 agtagttctt ttcttcaagc ttgtaagtgc cgtctttcaa atcagcacca ttgtcgattt
     2701 cgattgtatc tgtgttacca gcttgagcag cttggatcaa ttgttgtgcg tagttttgga
     2761 agctttcaga tgagtgagta gctccagtta cgacttccac accgcttgcg ctttgtgctt
     2821 ttaagaattg gtcattcaac tcaggaatgt atttttctgg gtttgtgcca gattttgctt
     2881 ccatgttttt gttgtattct gcatcttttg tttttgattc gccgttttca ttgacgttat
     2941 catattttga ttcagtgatt ttgccgtctt ttacaaccat ttcaaagact gcacggtaac
     3001 catttgaata atttttttct tctaatttat atgtgccgtc ttgcaaatct ccgccagcta
     3061 caacttttgc agatgattct gttgtgcttt ttgcagtcga agattgtgct gtagatgatt
     3121 cagaagaaga gtttgagctt ccgttgtcat ctgcgccgca agcgcctaac actagagctg
     3181 aaaatgctaa tacggcaaat ccagttacaa aacgtttttt cttcatttta ataccccacc
     3241 attccatttc tagtccacaa tttttctttg tgattcatta tacattctaa tctttttagc
     3301 ctataagtca actatttttg ataaataacc catgtaatat ttcaaacgtt ttctttcagc
     3361 aaatttcgtg aaaaaacgct ttattttaaa atttgaaaaa aatccaaaag gagttagaca
     3421 aaagaacaga acatctatat aatatatgga gattgtgtaa taagtatcaa actaaaagaa
     3481 ttatcatata ttcattatat agatagtcta taaggagcgg atgatcaatg gcaaagcaaa
     3541 atattgtcgt tgtaggagca ggatatgccg gtgtttcagc tacaaaatac ttggcaaaga
     3601 aactgaaaaa agaagatgta gcaattactc tgatcgatcg tcattcgtac catactatga
     3661 tgaccgagtt gcatgaagta gctggcggac gtgtagaacc agaagcaatc caatacgatc
     3721 tgcagcattt gtttgctcgt caaaagaatg tacaacttgt cacagacact gtaatcggta
     3781 tcgataaaga acgaaaaatc gtcaagacca aattaggttc ctatcctttt gatcagctga
     3841 tccttggaat gggcggagag ccgaatgact ttggtacacc tggtgtcaaa gcacacggat
     3901 tcaccctttg gtcatttgaa gattctgtaa ggattcggga acatattgag aagacagtag
     3961 caaaagctgc attggaacca gatgatgaac tgcgtaaagc catgctgact ttcgttgtct
     4021 gtggttctgg atttaccggt attgaaatga ttggggaact gatcgactgg aaaaatcgcc
     4081 tagccaaaga ttataaatta gatccaaacg aaatcacact gatggtcgta gaggcaatgc
     4141 cgacgatctt gaatatgttg gatcgaaatg atgctgcaaa agctgaacgc tatttgcgta
     4201 agcacaacgt ggaattatta ttgaatgcac caatcgtaga agtggctgct gatcatatca
     4261 aattgaaaga cggacgcaac attccgactc acacactgat ttggacagct ggtgtcaaag
     4321 gaacttctga cgctgctgac tttggattag aatcagcacg cggacagcgg ttgatcgcca
     4381 atgaatatat gcaggcaaaa ggctacgaag ataaaaacat ctatatcatt ggtgatttag
     4441 tcttttatga agaattccca ggaacaccga caccgcagat cgttcaagct gctgaacaaa
     4501 caggacacac agccgcagcg aatatcgtgg cttcgatcaa aggcggagaa aagcataaat
     4561 ttaaaggaaa ctatcaagga tttatggttt ctattggtgc caaatggggg gtagctaacc
     4621 tatttgataa gatccattta agcggatttt tagctatcgt aatgaaacat atcgtgaatt
     4681 tgaaatactt ctttgatatt cgttctggtt attatatgtt ccaatattta atgcatgagt
     4741 ttttccatat caaagatgat cgcaacgtga ctcgaggaca ttcttctcgt tacggcaatg
     4801 tcttatggag tgtgccactt cgtattttct atggtttagt ttggttcgtt gaagcttcca
     4861 aaaaaatcat tgggaatggt gaagtgttca aaccaagtac ttggtttggt gaaggatcat
     4921 ggttcactgg aaacagtgtg ttcccttgga catgggaata caatgtaaca gatgttgcca
     4981 gtggtgcttc tgctgctggt gatgcaacca cagctgccag cggagcaggg gcaagtgccg
     5041 gagaagcagc gactcaagct gcacattttg gtttgagtta tgcatacggt gaagaaccaa
     5101 tggctgtgat tgagaagtca ccagactggt tcacaaagat catgcaagtg atcatgccaa
     5161 acaatgatgt agcaatgttc ttccaaaaat tcatggtatt ctttgaaatt gccttggcat
     5221 tagcattgat tgctggatta ttcacatggc tatgcagtgc gacaacgatt gtattggtga
     5281 tcacgttctg cttatcaggc atgttttact gggtaaacat ttggttcatt tttgttgctt
     5341 tcgcattgat gaacggttcc ggacgtgcga ttgggttgga tcgctgggta attccatggc
     5401 tacaacgtac actaggtaat tggtggtacg gtagaccaag ttcccagtac ggcaagaaat
     5461 aaatagataa agaacgttat ttgaatgcaa gatttcaata gaaaggggct gagacaaacc
     5521 gtcacagcct cctttttatg taaaattaaa ggaataagat gcccgagata agcctccatg
     5581 agcaagacac aggtgtcaca acctctgtta caacctcttg ttataaacgg tgttccagaa
     5641 gtagcttctt cgaaaaataa gccgaaattt cacaaaaact tgaaaagtaa ttttcgtaaa
     5701 ttccttctta tttcttgaag ttgcccactt ctgtcacaac ctcttgttat aaacggtgtt
     5761 ccacctgctg cttctcggta ataagcccaa agaattagaa aacatggaaa gctgttttct
     5821 attcttcgtt cttattactc aaagcaagac acaggtgtca caacctcttg tttttatgct
     5881 aaaattgggt gagttaatct ggaaaaagga gaacaaaaat gagccttaaa gaatttgctg
     5941 aaaaaagtct gctcaaacct tgggatgttt ttattatttt gcttttgact gttctttctt
     6001 ttttaccagt catgattttt ggtcttcagc aaaacaccgc aacagcagaa aaagaagcgg
     6061 tactgcgtgt ggatggcaca gaaatcaaaa cattcccttt gatggctgga gaagagagca
     6121 acacatatac ttacaaagat gaacacggag attataactt gatcgagata gatggcgatc
     6181 gagtacggat aaaagaagca gattgcgatg accagatctg cgtacgtcga ggctgggcaa
     6241 cgaaaaatgg cgaaaccatc gtctgtttgc cacataaatt agtgatcgaa gtccgttcaa
     6301 cagatggagg tgacgaagac agtctgattt attagacctt cacacaccat gagtaaatta
     6361 caaaaaatga tttatatttc gctacttgtt gcacaaggag tgatcattgg tttattggaa
     6421 aatatgatcc cttatccttt ttcttttgcg cctggtgcaa aactaggctt agccaatcta
     6481 atcacgatcg ttgccatttt taccatgaaa aaacgagaca gcttcttact actttggcta
     6541 cgactattcc tgactacttt gctaggcgga acgatttcca cattccttta tagtatgagt
     6601 ggtgctttgc ttagttattt tggaatgtta ttggtcaagc aattaggacc aaaacgtgtc
     6661 agtatcatcg gaattagtgc ggccggcggt ttcatgcata atgtcggaca gcttttgatg
     6721 gcttctttca ttgcacaatc atggacagtc atgttgtact tgccagtcct ttcagtttta
     6781 gggattttat cagggattgc aatcgggatc gcagcgaatt atcttttacg acatgtgcag
     6841 acacttcaac gattccaaaa agattatgag cagggaacca aacaaaaatg gagcccaatg
     6901 tttaccaaaa aataatcgtc acacaaggag tttttttatg aagctacatc caatgtggga
     6961 aaactatcct gctttaagta aagaattgac agaaacacta aaacggatgg aagaaactgt
     7021 ccgcctgaaa aacaaacctg tcgaacaagc aatcaaagaa acgatccatg ccggcggaaa
     7081 gctgttgcgt cctgcctatc agctattgtt ctcacaattt ggaccagagc aagatcgaga
     7141 caaagccatc gctttggcag cttctatcga aatgctccat acggccacac tgatccatga
     7201 tgatatcgtt gatgaagcgg atctgcgacg tggtcagcct aatatccgta gtcagtttgg
     7261 taatgtcgtt tctgtctatg caggagacta tttatttgtc tgctgtttta aactattgtc
     7321 tgattactcc acttccttga aaagtctcca attgaattct cgcagtatgg aaaaagtatt
     7381 gaatggcgaa ttagggcaga tggatgatcg ctataattat gaactttctg taaaagaata
     7441 tttggaaaat atttcaggta agacagctga actgttccaa ttaagttgtt ctgttggcgc
     7501 ttttgaaagt ggaacaagcg agcgcttcgc aaaaaaagca ggagatatcg gactttctat
     7561 cgggatggcc tttcagatta tcgatgatat tttagattat acaaaagaga gccaggaaat
     7621 cggcaaacct gtactagaag acatgcgtca aggggtctat tctttaccgt tgatctactc
     7681 tttgcaaaaa aacaaaccaa agctactacc ttatttagag aagaaagcgg caatgacaga
     7741 agaagatgtc gacgccgtac gcaaaatcgt tgaacataca ggaggcgtgg aagaagctag
     7801 gaagttagcg gcctcttaca cagaaaaggc tttgaaggaa ataaagaaat tacctgccac
     7861 ctctcttcga acaaaagaga atctcttttc ccttacccaa ctgattttgg atcgcaaaga
     7921 ctgaataggg aaaggttcga tacgtaaata aatttctcag aaggaaatct acgtgttttt
     7981 tgacaaattg aattgtagtt cttgtattta tgataaaaag caaaaataaa gagcaacgtc
     8041 cgttagcgac gttgctcttt tttatcttga atattttttt accttttcga tttcttataa
     8101 ctcataaaag tgattcttaa accgattaca agtaatataa cgcctgcgag tgttatccaa
     8161 ttattcgatt gacttccagt tttaggaagt gctctttttg atttgtttcc attgtcattg
     8221 cttgttttat tagttaagtt aaagttcgtc ctgttatccg aacttgtttt tgagaaggag
     8281 acacttgcat gacgtgtatc gcttgtggta gacgacgtgt ctgtactatc gcttgaagtt
     8341 gatgaatctg agctatcact tgtggtagag gatgtatctg tactgtcact tgaagctgac
     8401 gtgtccgtgc tatcacttgt agtggtagat gaatctgagc tatcacttgt ggtagaggta
     8461 gtatctgtac tgtcacttga agctgacgtg tccgtgctat cacttgttgt ggtagatgta
     8521 tccgtactgt cgcttgaagc tgacgtgtcc gtgct
//
LOCUS       DADVXU010000107         8494 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.111, whole genome shotgun sequence.
ACCESSION   DADVXU010000107 DADVXU010000000
VERSION     DADVXU010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.111"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..173)
                     /locus_tag="KB953_002442"
     CDS             complement(<1..173)
                     /locus_tag="KB953_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBB6867375.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="KB953_002443"
     CDS             complement(593..1714)
                     /locus_tag="KB953_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HBB6867376.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="KB953_002444"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="KB953_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HBB6867377.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="KB953_002445"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="KB953_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HBB6867378.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="KB953_002446"
     CDS             complement(4304..5215)
                     /locus_tag="KB953_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBB6867379.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="KB953_002447"
     CDS             complement(5232..6239)
                     /locus_tag="KB953_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBB6867380.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="KB953_002448"
     CDS             complement(6236..8365)
                     /locus_tag="KB953_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBB6867381.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..>8494)
                     /locus_tag="KB953_002449"
     CDS             complement(8370..>8494)
                     /locus_tag="KB953_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBB6867382.1"
                     /translation="NGQCLMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1486 g         2681 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DADVXU010000108         8138 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.112, whole genome shotgun sequence.
ACCESSION   DADVXU010000108 DADVXU010000000
VERSION     DADVXU010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8138
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.112"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(101..949)
                     /locus_tag="KB953_002450"
     CDS             complement(101..949)
                     /locus_tag="KB953_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBB6867383.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1173..3113)
                     /gene="thrS"
                     /locus_tag="KB953_002451"
     CDS             complement(1173..3113)
                     /gene="thrS"
                     /locus_tag="KB953_002451"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBB6867384.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3450..3629)
                     /locus_tag="KB953_002452"
     CDS             complement(3450..3629)
                     /locus_tag="KB953_002452"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HBB6867385.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3676..5499)
                     /gene="cadA"
                     /locus_tag="KB953_002453"
     CDS             complement(3676..5499)
                     /gene="cadA"
                     /locus_tag="KB953_002453"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBB6867386.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5777..8056
                     /locus_tag="KB953_002454"
     CDS             5777..8056
                     /locus_tag="KB953_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBB6867387.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
BASE COUNT         2390 a         1719 c         1468 g         2561 t
ORIGIN      
        1 ttatgttttc ttaagtcgtg aaacactttg atggatcatt tatttgtgga aaactacgtt
       61 ttgttattct aaaaaactta tataaaaaat agtaaggttt ttatttcgaa tccataaaat
      121 attcttggat tgcttctcgt gtttttttca ctttttcgct aagcttttcg tttttagcca
      181 tatagaaata aaaaatgata tcgatcgtag caaattgggc caaaagtgaa ttagttgccg
      241 cacttcgatt cgtgatttct tttggtctag atgtttgtac gagtacatcg gctatcttgc
      301 taagcgggtt atttccaaat tgcgtcaacg caatgatttc gatattcatg cttttcgcca
      361 gctcggctaa tgcgactaca tctgcacttc tacctgagtt tgaaacaagc caaagcactt
      421 tctttttctc attgctgaca agttgcggga gcaagacgtg gatatctttt tcaaaaatga
      481 ttggcttacc gacacgagac catttttgca gaatatcttc tactactaaa gaagaggcgc
      541 caaccccata taaaaaaaca gtctctgccg attccaaaca ctctacgact ttttggatcg
      601 tttcttcttc tagaatatct actgtttctt ttatcgctac ttgtgcattg ttaccaagtt
      661 tgtttttgat agagtaaaag gactcatttg cttctatatc actaaaacct tcatcgattc
      721 cagtctgtag ttctgttgaa atcgtgattt tgaagtccgt cagactttga aaaccgattt
      781 ttttggaaaa acgtacaact gtcggcgcac tcaacccgga agcttctgct aattcattag
      841 ctgtcattgt agggaccttc tcaatattac cgataatgta gttggccaac tttttttctg
      901 ctgaagaata atcttccatc atactttcaa tacgactaat tacgctcata agttcctcca
      961 cccttttaat gatagtctat gtaagtatac catatcagac ccttattcac ggtcgtatac
     1021 tggataaaaa acaaaaaaac aatccgccca acagaatatc ttatcccatc aatcccacag
     1081 attctattga gcatctgtgt ctttgaggaa tacctgttat ctattgttcg gattgctttc
     1141 tatctactac ttttttacag gaaatttacc tattagtttt ctctgctgta atttttgact
     1201 tctgcttcca tagcatcaat aaagatattc aaatcttcta cagctgtttc tttacttcca
     1261 taacgacgta cgttcactgt tgcatcttcc aattccttgt ctccaacaac taattggtaa
     1321 gggatctttt gtgtttgaga ggcacgtatc ttatagccca ttttttcatt acgatcatca
     1381 acttctacac gcaacccctt catttgcaaa cgttctttga tttcataagc ataatcgcca
     1441 tgtgcatcaa cagaaacagg gatgattgtt gcttgtatag gtgccaacca tgttggaaaa
     1501 gcgcctttgt atacttctgt taaataagcg acaaaacgtt ccattgtaga aacaattccg
     1561 cggtggataa ctactggacg atgcgtattt tccccgtctt ctccaacata tgtcaaatca
     1621 aagcgctctg gcaataagaa gtctaattgt atagttgata atgtttcttc tgtaccaaga
     1681 gctgttttga cttgaacatc tagttttggt ccgtaaaagg ctgcctcgcc ttctgcttca
     1741 aagtaatcta gttctaattc atccatagcg gctttcagca ttgcttgggc tttttcccac
     1801 attgcgtcat catcaaaata tttatctgta ttgtttggat cgcgatagct caagcggaaa
     1861 cgataatctg taatattgaa gtcagcatat actgccacca tcaattcaag cgtacgttta
     1921 aattcatctt tgatttgatc aggacgaaca aatgtatgac catcattcaa cgtcatttct
     1981 cgtacacgtt gtaaacctga caaagcacct gatttttcat agcgatgcat cattcctaat
     2041 tcagcgatac gaattggtaa ttcacgataa gaatggatcg tgtttttgta gaccatcatg
     2101 tgatgagggc agttcattgg gcgaagaaca agcatttcgc catctcccat atccattggc
     2161 gggaacatgt cttcttgata atgatcccag tgtccagatg ttttgtacaa ctcaacatct
     2221 cccattattg gtgtatatac atgttggtaa cctagactaa cttctttgtc tacgatataa
     2281 cgttcgatcg tgcgtcggat tgttgcccct tttggaagcc agaatggcaa accagaacct
     2341 acttctgggg aaaccatgaa caagtcaagt tcttttccta gtttacgatg gtctctttct
     2401 tttgcttctt cacggagacg gatatattct ttcaaagctt tcttgtcaaa aaaggctgtc
     2461 ccgtatacac gttgcatcat ttgattgttt gagtttccac gccaataagc acctgccact
     2521 gataataatt taaatacttg tatacggcca gtggatggaa catgtggtcc acgacacaaa
     2581 tcgatgaatt cgccttgttg ataagcagta atcacttctt cttctggcaa ttcactaatc
     2641 agttctactt tgtacggatc acttgcaaaa atttctaatg cttcctgttt tgaaagaaca
     2701 cggcgttcga tcggcagatt ttctttgacg attttcatca tttctgcttc gattgccggc
     2761 aaatcttctg cagttacttg gttttgtcca ttatctgtat cgtagtaaaa ccctgaatcg
     2821 atagctggtc caacgccaaa gtggatattt ggatagaggc gacgcatggc ttgggccatc
     2881 aaatgagctg ctgaatgacg gactaagcca agtgcatctt catggtctgg tgtgatgatc
     2941 tcaatacttg catcttcctc gattggacga gtcaaatcaa cgagttttcc gttcacttta
     3001 ccagctaaag ctttttttgc taagcttttt gaaatgcttt cagcaattgc caatgttgtt
     3061 gttccagact caaattcttt gactgcacca tctggaaaag taatttttat cataatagtt
     3121 tcctccttga aatgttttga ttgatcagat catcaagctg aaaaacaaaa aaatcctagc
     3181 atctcttgaa aagagatact aggacgaaaa gttcgtggtt ccacctaatg ttcaaacgca
     3241 aatgcgttcc tcaagcgttg taacggtccg accgcccttg cttcaacaag ggtttcgaaa
     3301 gtggtcatcc tttggttttc tcctggaaaa gtttcagcct agcttttcct ctcttatcga
     3361 agactctcaa attcagttgg ctttctcatc aatcaattat gtgtttattt aaccactaat
     3421 aaatagaaaa atcaagtggg aaactaaact tattttttcc attccccatt tacaccgtat
     3481 gttccttttt tcatctcatt gatgatgaca tggatgtgtt cttttggtgc ctgcgtgttt
     3541 ttatgaacag cttccgtgat atctttcatc atattttcta attgttccgg actgcgacct
     3601 tctactaatt cgacatgtac gaatggcata atgctctctc ctctttctga tcgttctttt
     3661 ttcaactcga ttctatcata ctatgtttgt cttgggtact gaatcaggaa aaatggctgg
     3721 atcttttttt gctctcaagg cactcaaaat agagaccgta tccacgactt cttgcaaaac
     3781 agctccgaat aatgcgggga ttagtcccgt acttgcaatt aacatcaaaa aaatacaaat
     3841 aaagatgcca atcagtacag actgtttcgc tattctcatc gtatctcttg aaatcactac
     3901 tgcttttgct acacgcaaga gatcatcttt caagatgacc gcatctgcac tttcactagc
     3961 agctgtagaa ccatgagccc ccattgcaat accgatatct gcagcagcaa gtgctggtgc
     4021 gtcattcacg ccgtctccta ccatcgcaac aggtctttga tctttaggga tactttctaa
     4081 aatggtcagt ttatcttgag gcaagctacg ggcatgtaca gaatctaatt ggactttttc
     4141 tgcaatcgtt tgagcaatgg cagatccatc cccggtgatc atcatcaaat ttttcacatc
     4201 aagttttctt agctccttga ttgtctttgc tgcttctggg cgaatctgat ccgaaaatag
     4261 gatcgttcct gcaaaagatc gatcaattgt tacatagatt gctgtattgg acgtttctat
     4321 ttcttcagct gaaacaaaag aggcacgtcc aatctttact tcatgattat tgactactgc
     4381 ttggattcct tgaccggtca cttctttcag ttgactaaca ggatacagtt ggctgttttt
     4441 cacgttgttc actaacgaac gagctaagat atggctagat tcctgttctg cactagctgc
     4501 atacagaagt agctcttctt tttttattcc attgactggc tgaatcgcct ctacttccaa
     4561 tatccctttg gtaagcgttc ctgttttatc aaaagcaatc gtttttgtac gtgcgagctt
     4621 ttcaatcgtt gttccagttt ttacaacgat cccgttttta cttgaacggc tcattcctgc
     4681 aactaaagca atgggcgcag ctagaatcag tggacaaggt gatgccacta ccagtacctc
     4741 agcaaatcgt aaagggtctt ttgagaacca ccaagaaata ccaccgatca aataagcaat
     4801 caacgtgaaa gggactgcat aacgatcagc taaacggaca aatcgggcag gcttttcttt
     4861 tgattcttcg actagtttaa taatcatttg atactggctg tctgcggcac gtttttcagc
     4921 gatcatttgg atcgatccgt ctccatttaa agaccctgac ataatctgat ctccaggttt
     4981 cttggtttcc gggcgggact cacctgtcaa agaagattca tcgaccgaag attctccttt
     5041 ggtcactcga ccatctgcgg gtacaagttc gccaggcttg actagtaact ggtcatcaat
     5101 atttatttca tcgattacta tatcctgcaa ttttccttct ttgaatcgat gggcaattct
     5161 tggggtgtta tctaacaaag actgcagttc tttacccgct cgtttattcg cgtaatcctc
     5221 tagactatct ccgcccgtaa gcatgatcaa tatcatcaaa ctagcccagt attctttgac
     5281 ggctaatgtt gcaaggattg cggtaatcgc taatacatct acgccatatc ttcctgaacg
     5341 taacgtcttg atcatttcca aaaacatcaa gaaggcgaga atccctccag taaatgcgac
     5401 aatcgcaaat gcaagggccg gctggtgaaa aacaaattca cttaataaag ctatcccacc
     5461 aataagcaaa gtaagtaaaa attttttgaa atgtgtcatt gttttttctc ctcttttatg
     5521 tgacttagag aggttagtaa taaatttcca gctgcctctt tcatcttaag catagtcctt
     5581 gactagcact ttttaaaaca ataggcctta attgaaaaca actatcacta tcagcgtttc
     5641 caaaattcat cagttctaca aacaaaaata ttcagcaagg attacttttt cgattcatca
     5701 gttaaaaagt accttttttc gaaaaactat gctatacttt taattcgtta tttacggcgg
     5761 aaggagcaaa catgcgatga atgagaaaca actagaacaa acgcacgtgg atgaaacgat
     5821 cgaactaatc cagatcgaac aaaatatgct ggatacaaaa caacaagaat tgactcagga
     5881 aatgaatcat tcgacaaaag acacagctaa tcacaagatt cgagctggtt ccaatgaagc
     5941 tttttatgaa tctgctgttg aatatcgcca acatgagcag gaattgcttt tgaaatacca
     6001 tactcttgaa gcacagcaaa aacgcctgca aacgttgtct gtcatgaaag atagtcctta
     6061 ttttgcccgt atcgacttta aagaagaccg tgaaacagaa acattatatc taggtatcgc
     6121 ttctttaaga gatctggcag aagaaacaat cgttattgac tggcgcgcac caattgccaa
     6181 tctttattat gaaggtgaga ttggtcccgc attttatgaa actgaaacag atagaataga
     6241 tgtcgatctg cagttgaaac gacaatttaa aatccaagaa ggacgtattt tatctatggt
     6301 agatacgtcg gaagtaatca atgatgactt tttgctagag atattggatg aatcatcaag
     6361 caaccatatg aaaaacatcg tctctacgat acaaaaagca caaaatgcaa tcattcggga
     6421 cacaacaagt aaagtaatgc tgattgaagg aatcgccgga agtggaaaga catccgcttt
     6481 gcttcaacgt attgcttttc tgttgtatca taatcgcaag tggctcgagg ctgaaaacgt
     6541 tcttctattt tcacccaacc atttgttttc tgattacatc tcgactgtat tgccttcatt
     6601 aggtgaaagc ggcgtgccta cacagacatt caaaaattat ttggtgcagc tgctgccaga
     6661 gttttcttta ctagatgaac agcaacaaga aagtggtttt ttgctagggg aaaaagatcc
     6721 gatacaagtg atgaaaagcg gattgacctt agtcgatcag attgatcgct atatccaagg
     6781 catcacaaac tacggtccct tgttccgtga tatgaaaata aatggccgta cgatcctatc
     6841 aaaggaatcg attcgccaat ggtataaaga aacaaatgaa caattacctt tacatcatag
     6901 actttctcta ttacaaacga agctcctgaa aaaattaggc ggtctgcaaa aagacgaaac
     6961 acgtcagcaa tgggtcaaag atctggcgga agaacagctc caagaactat acgcaacaga
     7021 tccaaatctt gaatatacag aacaaaagga gcgttcatta cgtaaaaaac tggccaacca
     7081 aatcgtacgt aaaagattcc gtcgaatcca tcgagggatc atgaattatc aatttgtcaa
     7141 tataacgaaa caatatctcc actttctaca aactgtgcca ctttctcttc taacacgaca
     7201 taaaatcagc gagaccaggt ggcaggaaca tttgacaact gttcgtacac acttgcgtca
     7261 aagagaattg cgtcaagagg atgctgtact attcttttta ctgatgcgcc gaacatatcc
     7321 agtgacggtt acacacaaag ctcgctatat ctttattgat gaaatgcagg atttcgcacc
     7381 atcacaagtg gcattgctgc aagccttgtt cccaaaagca aatttgactt tctgtggaga
     7441 tctaaatcaa aatgtctttg gaaatgaaac gatcgttgga tcattggaag accttttcgt
     7501 ggaccgtgaa gtcactcgtt tccaattaac gacaagctat cgctccacaa aagaaatcac
     7561 tgattttgct aatcatttta ttgtgaaaga acatcaagta gaaacaacag caagaaaagg
     7621 agaccttcct ttgctcatcc aaggaacaac agatgatatc aaattgaatt ggttagcaga
     7681 taccattcga acgactcagg gaaaagcccg gtattggcgt acagcaatca tagctaaaac
     7741 acaagaagaa tgcgaagctc tttatgctca actgccagac gagttgaaac aacaagtcca
     7801 gctgatttta aatgagagtg actttatgaa gcgttccatt attttacttc cagccttttt
     7861 agcaaaaggt ctggagtttg accgagtttt cttgtgggat gtagatgcag aacatttttc
     7921 ttctcagcaa gacaaattga tcctttacac catgtgtaca cgtgcgatgc atgaattgac
     7981 cctttttacc tccaataatg aaagtccttt gttaaacaat atggatattt catcttatga
     8041 gaaaaaagta ctgtaagtaa aaagacaact gctatcatta atgttagcag ttgtcttttt
     8101 atttacaata ataattgcag aaaaagtaat tcaatcat
//
LOCUS       DADVXU010000109         8078 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.113, whole genome shotgun sequence.
ACCESSION   DADVXU010000109 DADVXU010000000
VERSION     DADVXU010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.113"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(80..262)
                     /locus_tag="KB953_002455"
                     /pseudo
     CDS             complement(80..262)
                     /locus_tag="KB953_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            279..446
                     /locus_tag="KB953_002456"
     CDS             279..446
                     /locus_tag="KB953_002456"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867388.1"
                     /translation="MENTFELYNKTTVDKLIPILVPTDLNGHDVLLRFNGFLGEDYRY
                     LKISDPQPLTE"
     gene            complement(489..1064)
                     /locus_tag="KB953_002457"
     CDS             complement(489..1064)
                     /locus_tag="KB953_002457"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HBB6867389.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1219..1745)
                     /locus_tag="KB953_002458"
                     /pseudo
     CDS             complement(1219..1745)
                     /locus_tag="KB953_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2202..3713
                     /gene="gadC"
                     /locus_tag="KB953_002459"
     CDS             2202..3713
                     /gene="gadC"
                     /locus_tag="KB953_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HBB6867390.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            3726..5126
                     /locus_tag="KB953_002460"
     CDS             3726..5126
                     /locus_tag="KB953_002460"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBB6867391.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(5291..6726)
                     /locus_tag="KB953_002461"
                     /pseudo
     CDS             complement(5291..6726)
                     /locus_tag="KB953_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            7119..7712
                     /locus_tag="KB953_002462"
     CDS             7119..7712
                     /locus_tag="KB953_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HBB6867392.1"
                     /translation="MNKDITFPVMDVRLVPITKIQANDYNPNRVASPEMNLLELSIIE
                     DGFTQPLVCYYKEKEEKYILVDVLHRYRVAKERLNLKVVPVTVIDKPIEERMASTIRH
                     NRARGTHEIRGMEIVIQRLVKNGWSDKRISKELGMDIEEIFRFKQVSGGMATVFLTNF
                     IRCRTSFRMLFRLSLTMSLLGCDDLRQELTVKVRLPR"
     gene            complement(7696..>8078)
                     /locus_tag="KB953_002463"
     CDS             complement(7696..>8078)
                     /locus_tag="KB953_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBB6867393.1"
                     /translation="FSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKG
                     CPFDNAVAESTYKSLKVEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVG
                     YRNQRLAQRILDNELGCANASEAV"
BASE COUNT         2515 a         1389 c         1477 g         2697 t
ORIGIN      
        1 ttctcaatct ttttaccact ccaaacaggg agcgtcaata attttgtgta aataactcgt
       61 ccttctgcaa aataatgggt tactcagtaa acattgaagc taatgtatcc gtcacttgtt
      121 ggaagccttt atggcttcta tttaagaatt tttgattgta tgtatcaaag atggatacta
      181 ggaaacgctc tagtgattct tcattttgaa actgctcttt tctgcggctg tatttcttga
      241 tttgtttatt gaaagattcg attgtagaac cagaaggaat ggagaacact tttgaacttt
      301 acaacaaaac tactgttgat aaattaattc caattttagt tccaactgat ttaaatggtc
      361 atgatgtttt acttcgtttt aacggatttt taggtgagga ttatagatac ctaaaaattt
      421 ctgatcctca acctttaaca gaataataaa aaacgtgtaa tcaattattt gattacacgt
      481 tttttatttc aatcttcgtt gtagtagtgg ttgcgacata ccgaaaggta atcttcatta
      541 cctcctacta caatctgttc acctgaataa actggaatac catcaacaat acgagcattc
      601 attacagcct ttttattgca aaaatggcaa atagttttaa tttcctcaat tttatcagca
      661 aatgtcaata atgcttcgga tcccttgaag agattatttt gaaaatcatt cttcaatcca
      721 tagcaaatta ctggtacttc ctcattcatt acgataagtg atagatcaat aatattctct
      781 ctacttaaaa attgagactc atcgaccaaa atacaatcaa cttttccgta ctgatcaata
      841 atctccacta attctccgta cacatccatt tcagaagtaa ttgcaatagc tggtttcgaa
      901 acacctatac gactctcaac ttttccaaat cctgatcgag tatctattgc gcttgtcatt
      961 atgataactc tcttcccttg ttcttcataa ttatgagcca ctttcagtag ctcaatactc
     1021 tttccactat tcattgttcc atatctgtaa aataattgtg ccattaaatt tattcccttc
     1081 ttttacaatt caattattcc ttaattccat ctgcttttat gcagctgctc gtttattcct
     1141 tgatatgtct ataacatcta ctatccctaa aaccaattta taagccatta gagatggtat
     1201 tcctgaacat ctctcatatt gttttttgag tggtatatca tctgaaaaac tgaaggaatc
     1261 atgaaaatag cgacatactt atcatttaga gttctaactt taaatgcatt tacttcttca
     1321 gtcagcactt tagtcatgtt ggacatggtt tggggcgtgt agtgatgccc atacattttc
     1381 tcaatcaagt ctgcgatttc cgacatggta ataccttttc ggaagaggtg aatgacggtc
     1441 tcctctaatg tgtcattcgt ccgtctataa gcaggaacag tctgttgctt gaactcgcca
     1501 ttgcggtcgc gcggaatctg gagatgaagt tccccatact cggtcttgac cgtacggtca
     1561 taggagccgt tacgagaatt acccgtgtta aaaccaatgc gatcgtactt ttcgtaatcg
     1621 aggaaagccg ttaactccgt tttgagaagc gtattgacag ctttttccaa atggacacgg
     1681 aataatccat tcaaatcgcc tttgttcgct agagtcttta ggatttctgt agtaaaatcg
     1741 ttcatatgga agtcctcttt tctgtgaatg tgttgtggta actctattct acagaaggga
     1801 cttccttttt tctatttaca caaaatattt tacactctca acaaaagtat cttcgattaa
     1861 tacttgctca agttatgtgc agctacgaaa aagtaggtac cgaggtactt tctttttcgc
     1921 agcttttttt ggacttatga gaagtacatt cgctggaaga atacgatata aaacgagctt
     1981 tttcactaaa gatattaata gagtgaaaga aacaagcttt tgttgtcagt tttttcactt
     2041 ttctaaaaat gaaaataaaa aattctggaa gtggagaaga attttgcttg ctatactttg
     2101 tcttgtagaa aagaggtaga caaaataatg caagctcaaa taagcgattt tccataacct
     2161 tggtaggaat gacaaattaa ataggaaagg gaagtaataa tatgaataaa aaaaagttat
     2221 ctctattcgg tttttttgcc ctgacagctt ccatggtttt gactgtttat gagtatccga
     2281 cctttgcaac atcacaatta catttggttt tctttcttct tttgggaggt attttatggt
     2341 ttctaccagt tgctttatgt gctgcagaaa tggcaacagt tgaaggatgg aaagatggtg
     2401 ggattttcag ttgggttagt caaactttag gagaacgttt tggttttgca gctatttttt
     2461 tccaatggtt tcaaattaca gtaggtttta ttacaatgat ctacttcata ctaggagcgc
     2521 tgtcgtatgt aattaatttt ccagagctta atacagatcc atttatcaag tttattggtg
     2581 tattagtcat tttctgggta ctgacttttt cacagttggg cgggaccaat cgtaccgcta
     2641 aaattgctaa agcaggattt gtcattggaa ttgttattcc ttcgatcatt ctctttggtt
     2701 tagcagcagc ctactttatc ggcggtaatc cgattcaaat ggctattaat gcgaaagctt
     2761 ttattcctga cttttctcag ctttcaactt tagtagtatt tgtatctttt atattggctt
     2821 atatgggcgt agaagcttca gcatcacata ttaatgagct ggaaaatccc caaagaaact
     2881 acccattggc aatgttttta ctagtgattt tggccatcgc cttagatgca atcggcggtt
     2941 tctcagtagc agcggttatt ccacaacatg acttgtctct cagtgctggt gtaatccaaa
     3001 catttgaaac gcttatttta cattttaaca gtcatttaca ttggttggta aaagtgattg
     3061 ctctcatgat tgcctgtggc gttatgggtg aagttagttc ttgggtagtt ggtccatcac
     3121 gagggatgtt tgcagctgct cagcgaggtc tgttaccagt tttcttccgg aaaactaata
     3181 aacaagacgt tccagttccg ttgattatga tacaaggaat tatcgttact atttgggcag
     3241 cagtcttaac ttttggtgga ggaggcaaca atttatcatt cttagtagca atctccttaa
     3301 ctgttgtcat ttatctcgta ggatatttac tatttttcct aggttacttt gtattagttt
     3361 ttaagaaaaa agacttaaaa cgaacttaca atgtaccagg aaaaactgtt ggcaaaacga
     3421 ttatcggtgg aattggtttt attttatcag tgttcgcatt gcttatctct tttgtaccac
     3481 cagcttcaat tgcagctagt gagcaccgaa cataccaaat catactactt attagctttg
     3541 tagtgactgt tcttttacct tttatcattt atgaagtaca tgataaaagt gctcacacaa
     3601 ctattgagga acctactcac gtgaaagctg aagacgttaa tcccgctgtt tatccagctg
     3661 ctagaggaga gcaccacgtt acaaaaaatg atgaacatat tttaaaacat taggaggaat
     3721 tcattatgtt atacggaaaa gataatcaag aagaaaaaaa ctatttggaa ccaatttttg
     3781 gctctgcaag tgaggatgtt gacttgccta aatataagtt aaacaaagaa tccattgaac
     3841 cacgaattgc ttatcaatta gtacaagacg agatgttgga tgaaggaaat gcgcgattaa
     3901 acttagctac tttttgtcaa acgtatatgg aacctgaagc agtgaaattg atgacccaaa
     3961 cgttagaaaa aaatgcaatt gataaatcag aatacccacg aacaacggaa attgaaaacc
     4021 gctgtgtaaa tatgattgct gatttatggc atgctccaaa taatgaaaaa ttcatgggaa
     4081 cttcaacgat cggctcttca gaagcctgca tgctgggtgg tatggccatg aaatttgctt
     4141 ggcgtaaacg tgctgaaaaa ttaggtcttg atattcaagc aaaaaaacct aacctggtga
     4201 tctcttctgg ttaccaagtt tgttgggaaa aattctgtgt atattgggat gtggaactga
     4261 gagaagtccc aatggatgaa aaacatatgt caattaatct agatactgtc atggattatg
     4321 tggatgagta cacaattggt attgtaggta ttatgggtat tacttacact ggtcgttatg
     4381 atgatatcaa gggtctgaat gatttagttg aagctcacaa taaacaaact gactataaag
     4441 tatacattca tgttgacgct gcatcgggtg gcttttatgc accatttact gaacctgatc
     4501 tagtttggga ttttcaattg aaaaatgtta tctcaattaa ttcttcaggt cacaaatatg
     4561 gtttggtata tccaggtgtg ggttgggtct tatggcgtga ccaacaatac ttaccagaag
     4621 aattagtatt taaagttagt tacttaggtg gagaaatgcc aactatggct atcaacttct
     4681 ctcatagtgc agcacaacta attgggcaat actacaactt tgtacgctat ggctttgatg
     4741 gttatcgtga tattcaccaa agaactcatg atgttgctgt ttatttagcc aaagagatcg
     4801 aaaaaactgg tatttttgaa atcattaatg atggatcaga attgccagtt gtgtgctata
     4861 agctgaaaga agatcccaat cgcgaatgga cactatatga tttatctgat cgtctgttaa
     4921 tgaagggatg gcaagtccca gcctacccat tacctaaaga cttggatcaa ttaattattc
     4981 aacgcttagt tgttcgagca gactttggaa tgaacatggc tggtgattat gtacaagata
     5041 tgaaccaagc aattgaagag ttgaataaag ctcatattgt ttatcataaa aaacaggatg
     5101 tgaaaacgta cggctttact cactaaaatt cttcagagaa atataaaaga agttaattaa
     5161 ctaataaaag gagttgtgat taccacacag ctccttaaat ctatttgaat aaatatatga
     5221 tattcatttg ttacactctc aatcttagct gttgttctat tcatcattac tcttattgta
     5281 agtagttcag tcaaatttgc ttgttcaatt gatcgataac agtatgaatt gtttgaaaag
     5341 taatcggata tataattgtt ttctcactac ctaagccttc ttggtataaa gaactatcgc
     5401 aaagtataat ccgctgagta caaaagaaag tgtcgtctac tatttctcta agttgtttac
     5461 aaatgatatt actaatagaa aagtgttctc ccaaaatgtc ttctaactgt tgtcgatagt
     5521 ataaatacct aaagtctgac ggagctacta tgaacacttc aattggagat ttccgtttat
     5581 gctcatttaa aagacttaaa ctaatggtta gtttacgaat agaattctca ttccaacgca
     5641 gaaataaatt gttttgtttt cgccattgtt caagaatttc atgaacttct aagtacaaag
     5701 cgcgttgctc ttcatttaac aagtaagtct tatcaggttg aaatatctga atatcagccc
     5761 aaagtgagcg gataaacggg agaagtgctt tatcaaaaag tatctgattt tcttcagata
     5821 tattcagaga taagataagt ttttgactga gctcaatcac caaagggtta tctgctacat
     5881 ggttttgata aacaacgttc aagtcttttt gtaacaattc aaagttatcg catagataat
     5941 ttctagagtt gaatagaaag tagatataaa ggcattcatc ttcgtcatga cataaagaga
     6001 aagaaagttg ctctaatcct ttctgtatta gctgaaacaa gggagtatgt cgggcaactc
     6061 ttttttcttc ctgactaaaa tatattgggt tagtcttaga tctctgcaaa ccgatagcga
     6121 tacctaataa aataaattgt ttttcaactg tagggtaact tcttgaaaaa aatgtttttc
     6181 tacataatca atcaattgtt gaccgctttg aataattgat ttatttagct ggatcaactc
     6241 tgtccgattt gtctcataga ttatcgttag taacaaactt cgataatcct tttcaggaat
     6301 ttcaaaatgg ccatcagcat ttaagtactg catttgttta aaaaaattaa tcaactcatt
     6361 tcgtaagttg taaactttgc ttaacgaaat gaaggtagta tcagctatat tactccaatt
     6421 gtgttcccct gataaataat gtcctagtac attgaaaaag tcagagcgct gatagatgag
     6481 ttgagtgagt gccaacctcg aaaacgaaga gtcgaatgcg actgtaaagc tacttctaga
     6541 tttctccact gaaataattt gctgatggag tatgtcggta atgcattcca agtcgttagt
     6601 gacagtgagt gggcaaacgt tcaattgatc tgctatttcg cgaatctgaa tggtcccttt
     6661 ttcgtgtaat tgctcacaaa tgtaaacttg acgaaaaata cttttctcca gataggtttc
     6721 caacatagta ctcctcctag tcattttata cataactatt ataactctac caatcggaat
     6781 agtcctgtaa tatattgaat caaaaatata aattataaaa taattagatg gggtatttgg
     6841 tttaagtatc ccttatatag taataaattt ataggtgtga ttctcgaatt aatacccgaa
     6901 aatgtgagag aatttttatg gattttttag gatacaaatt aattattttt gcttttatcc
     6961 tgattaccag tccagtactg tcggtatccc attttgcagc aaaatcatgt tgataaagtg
     7021 acaagaagac tgatgagata tattatccaa attgagaaga taattaatcg gattcttgag
     7081 tataccctac tttttaagaa agtagggtat acaagcgtat gaataaagac ataactttcc
     7141 ctgtgatgga cgttcggctt gttccaatta ccaaaataca ggcgaacgat tataatccca
     7201 atcgagtagc gtctccggaa atgaacttat tggagctttc tatcattgaa gatggcttta
     7261 ctcaaccatt agtttgttat tacaaggaaa aagaagagaa gtatatcttg gttgatgtgc
     7321 ttcataggta tcgtgttgct aaggaaagac tcaacttaaa agttgtaccg gtaacagtta
     7381 ttgataaacc catagaagaa cgtatggctt caacaattcg acacaatcgt gcaagaggga
     7441 cccatgaaat aagaggaatg gaaattgtca tccaaagatt agtaaaaaat ggatggtcag
     7501 acaagagaat atccaaagaa ttaggcatgg acattgaaga aatttttcgc tttaagcaag
     7561 tgtctggtgg aatggcaaca gtttttttga caaattttat aaggtgcaga acttctttcc
     7621 gtatgctatt ccgattgtcc ttgacaatga gcctcctcgg atgtgacgat cttcggcagg
     7681 agctaacagt taaagttaga ctgcctcgct agcgttagca catccgagct cattatcaag
     7741 gattcgctgc gccaatctct ggttacggta accaaccggt gtctcgtagc caagtgtacc
     7801 gtgcaaccga aggtggttcc accaatttac atagtcaaat aactccaaat ccaattgttg
     7861 taaggtttca aatgtgtatt gatagacaaa ttctactttc aacgacttat aagttgattc
     7921 agctacggca ttatcaaaag gacagccttt atgactcaat gatcgattga tgtcaaaagt
     7981 tgttaataat tcatcaatag cttggttatc aaactctttt ccacgatcag tatgaaaaat
     8041 ctcaacctct gtcagaggtt gtttgatacg gctaaatg
//
LOCUS       DADVXU010000110         7992 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.114, whole genome shotgun sequence.
ACCESSION   DADVXU010000110 DADVXU010000000
VERSION     DADVXU010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.114"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            267..2033
                     /locus_tag="KB953_002464"
     CDS             267..2033
                     /locus_tag="KB953_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HBB6867394.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2135..3394
                     /locus_tag="KB953_002465"
     CDS             2135..3394
                     /locus_tag="KB953_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBB6867395.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3541..4842
                     /locus_tag="KB953_002466"
     CDS             3541..4842
                     /locus_tag="KB953_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBB6867396.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4845..5696
                     /locus_tag="KB953_002467"
     CDS             4845..5696
                     /locus_tag="KB953_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBB6867397.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5709..6524
                     /locus_tag="KB953_002468"
     CDS             5709..6524
                     /locus_tag="KB953_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HBB6867398.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6675..7841
                     /locus_tag="KB953_002469"
     CDS             6675..7841
                     /locus_tag="KB953_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBB6867399.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
BASE COUNT         2609 a         1378 c         1591 g         2414 t
ORIGIN      
        1 gaatatactt ctacgccaaa aactgttaaa aaacttttac tttagaaaat tggagaaatt
       61 gatcgcttaa gataaattca ggacactaaa tagtacagga agtagactaa aacttttaaa
      121 agttttaagt ctatctattt ggcaagttta aaaaataaaa attcggtatc aaaaaactgt
      181 taccgataac atttttgaaa gcgttgtctt ttgcttctct atcacttata gtgatgacgt
      241 catattaaaa cacaaggagg aaatcaatgg atacagctgc aatttatcat cgaccggaaa
      301 gtgaatatgc atttctatat acgaatacaa gatttcgtat tcggcttcgt actaggaaaa
      361 atgatatcaa aaaagtatat gtgctatgtg gagatccata taccataaca actgaaaaat
      421 ggtatcaaaa acaacgacca atgaaaaaat gtctaagtac caatgttcat gattattggg
      481 aaattgaagt gacgagcgaa acaagtcgct tacagtatgc gttccatgtt gttggggaag
      541 ataacacgga ctgtttttac ggtgatcaag gaatttttcc ttatcgagaa aatgtgatta
      601 ctgaaccaaa tttttatttt cgaatacctt attttcatca aatcgatcga ttcactattc
      661 cagaatgggt aaaagaaaca gtatggtatc aaatttttcc cgaaagattt gcaaacgggg
      721 acaaagcaaa tgaccccgaa cacactttac cttggggaag taaagatcca gaccgagaag
      781 actttttcgg gggagatttg caaggtgtga tggatcattt ggattacttg acggaccttg
      841 gcatcaatgg catctatttt tgtcccatat ttaaagccac atccaatcat aaatacgata
      901 cgattgatta ttatgaggtg gatcccgctt ttggcgataa aaaactttta aaaaacttga
      961 ttgatgaagc acataaaaga gggatacgta tcatgttcga tgctgtattc aatcatatgg
     1021 gtgtccattc accacagtgg caagacgttc tcaaaaacgg agaaaaatcg atctataaag
     1081 actggttcca catccgtttt ttcccagtag attcttatca gatgactgac ttaccagaaa
     1141 ctgctgaaaa tatcccatat gatacttttg cttttactcc attcatgccg aaactaaata
     1201 cagcaaatcc agaagtgcaa aaatatttgc tggatatcgc aacttattgg ataaaagaat
     1261 tcgatattga tggatggcgt cttgatgttg ctaatgaagt cgatcatcat ttttggaaaa
     1321 aattcagaga agcagttaca gaaatcaaac cagacatcta tatccttgga gagatttggc
     1381 attcctctca agcttggctg caaggggatg aattccatgc agtgatgaat tatgcattta
     1441 cagattcgat taaagattat tttgcaaaaa agaaaatcac agcttcacaa atggtcagtg
     1501 gaatgaatca tcagcaaatg ctttacagag atcaggtgaa cgaaggaaca ttcaacttat
     1561 tagattctca tgatactgcc aggatcttga ccctttgcca aggtaacaaa gaattgatga
     1621 agtctgtgat ggcctttatg tttcttcaaa aagggtctcc ttgtatctat tacgggacag
     1681 aaatagggat gactggtgaa gatgacccgg attgccgcaa atgtatgata tgggagaagg
     1741 aggaacaaga tctagagcta ttcggtttta taaaagaact tgtttctcta aggaaacagt
     1801 tgtctaagat tatttctgaa ggttctactc agtggctaat agtaaatgat cgtgaagata
     1861 agctctattt cacacggaaa ctagagggac agatcatcta tgtttacttt aatcagtcaa
     1921 aagaaccttg ggtagtagaa caggaaaatg aggtgattct tagtcaaaat tgtcagttgt
     1981 tagaagacgg gaaagcagag atacgacaat acggattttt gatcatgtgt tgaaaactgt
     2041 taccggtaac aacaagaaag cgtttgcaaa tgaagatttg tgcccattat aataagggtg
     2101 taacagcacg ttgaatacaa aggaggaaat gaagatgaaa aaaagaagtg tgaaacagtt
     2161 gggtttaggg ttaatctctt taggaatctt aggcggatta gcggcttgtg gcaatggtaa
     2221 ctcttcagca gaaggggaag ccgatgaagg aaaaacgtta acagtttccg ttgattctgg
     2281 atataaagat tatataaacg aaataaaaaa cactttcgaa aaagaaaacg atgtgaaaat
     2341 caagctagta gaaaaggaca tgttcgatca gttggaatca ttagcgttag atgggcctgc
     2401 tggaaaggga cctgacgtta tgatggcagc atatgaccgg atcggtgcgt taggacagca
     2461 aggacatctg gcggaagtaa aactgggaaa tgaagcagct tacgatgaaa cagacaaagc
     2521 ccaagtgact tatgatggaa aaatctacgg agaacctgca gtaatcgaga cccttgtttt
     2581 gtactataac aaagatttag tcgatacagc accatcaacg tttaaagatt tggagaatct
     2641 atccaaagac agtcgttttg cttttgagtc agaagcagga aaaaatacag gatttttagc
     2701 taaatggact gacttttatt attcctatgg tttaattgca ggttacggtg gctatgtatt
     2761 tggtgatgat ggcacaaatc cttcagatat cggtttgaat aatgcaggtg ctgtagaagg
     2821 aatctcttat gcaacagatt ggttcaaaaa tgtttggcca aagggaatgc aagacataaa
     2881 aagtgcagga gacttcgcca gccagcaatt catgtccaat aaaactgcag caatcattga
     2941 cggtccatgg caagcgcaaa catacaaaga aaacaatatc aattatggtg tagccaaaat
     3001 tcctactcta aataatggac agccttatca accgtttggc ggaggaaaag gatgggtagt
     3061 cagcaattat gctaaaaaca aagacctctc tcaaaaatgg cttgattatg taacgaatca
     3121 ggaaaatcaa gaaaaattct ttgaaatggt aaatgaaatc cctgcaaacc aacaagctag
     3181 ggaaacagca aaggctaaaa acgatgaatt aacaacagct gtgattgaac aatatgaaac
     3241 agctcaagcg atgccaaaca tcccagaaat gggcgaagtt tggacaggtg cagaaaactt
     3301 gatgttcgat gcagcatcag gttcaaaaac accaaaagaa tccgcagatg aagcagtaaa
     3361 aacaatcagt gaagccatag aacaaaaata ctgattaaaa tgataaccgt ccatttttat
     3421 aaaaaatgga acaggtgctt cagataaaag tggcaagagc gagatggaaa attatcttgc
     3481 tcttgttgcc attatcttaa aagcagtttg cggtttagca agtttagagg gggcattcag
     3541 atgcagcaag ataaaacaag caatatcaac gaaaaaaaaa tcacgctttg ttcacttatt
     3601 ccagggatgg ggcagtttgt aaatggccaa gcttttaaag gtatcgtttt tttcgccata
     3661 ttcgtcctat tcattggtca gatgcttctg ggcggatttc atgcattggt aggattggtt
     3721 actttgggaa gtataccaat ggaagatcat tcattattta ttttgattga aggcactttg
     3781 caattcatca ttacaatcat tttcttgatt ttttacggtt tgaatctttt agacgcttat
     3841 cgagtagcga agatgcgtca agagggcaaa aaaatcaatc ggacgatgaa ggaagtcata
     3901 cataatatag gtgatgaagg ttttccttat ctattgacac ttcccgctta tcttctcatg
     3961 atttttacga tcattttccc agtcctagtt acattgttca ctgcatttac taattatgac
     4021 ttcaatcata ttccaccagc cagtttaatt gattgggtag gggctaaaaa cttttttagt
     4081 atcttctttt taagctcata tcgtaatact ttttttgcag tcttttcgtg gaccgtgatt
     4141 tggactttat gtgcaacaac tttgcaaatc acccttggta ttctcacagc ggttgttgct
     4201 caccagaaat ttattaaatt caaacgtatt ttcggtatca ttttcttatt accatgggcg
     4261 gtcccagctt ttattactat catgagtttt tcgaatatat tcaacgatag cgtaggtgcc
     4321 atcaatactc aagtgattcc tttcataaac cagcttcctt ttgtagatat cagtgcaatc
     4381 tcttggaaaa cggacccgaa ctggacgaaa gtagcaatta tcttagtgca gggctggtta
     4441 ggatttccct atatctatgt gatggttaca ggaatcttac aatcgatttc agaagactta
     4501 tatgaagcag caaaaatcga cggagcaaat gcatggcagc gcttttcaaa catcacgctt
     4561 cctgctgtct tcctagtggc agcgccaaca tttattacac agtatacggg gaatttcaat
     4621 aatttctcca tgatctatct gtttaacaat ggcggtcccg gaagcgtagg cggaggagct
     4681 ggttctactg atattcttat ttcctggatc tataaactta cgactggaac ttctccgcag
     4741 tattctatgg cttcagcaat cacgttgatt atctccgtca tcgttatctg tgtgtcattg
     4801 ctggtattta aaaagacaaa tgcgttcaat atgggggatt aacaatgaaa aaatttcatt
     4861 cgagtgctcg attccaaaaa cgactttctc acttttttac ttactttttt cttttaatac
     4921 tgtcagtcat tatcatctat ccgcttttga tcacagcaag tactgccttc aagtcaggaa
     4981 atattgcagc ttttagttta gatctttcaa gtgactggac tttaggaaac tttactcggc
     5041 tattcagtga aacactttac gggacttggt ataaaaatac attgatcatt tctgtcctaa
     5101 caatgatcgt acaagtgact gtagtaactt tggcgggcta tacttatagt cgctatcgtt
     5161 ttatcggccg caaatcaagt ctgatgttct ttttagtgat tcagatggta ccgactatgg
     5221 cagcattgac tgccttttat gtcatggctt tcttattaga agcgctcgat aagtactggt
     5281 tcttgacaat gatctatata ggcggtggaa ttccaatgaa tacttggctg atgaaaggat
     5341 actttgacac tgtaccaata gatttagatg aatctgctaa acttgatggg gcagggcatt
     5401 tcaggatttt tttccagatt gtattaccgc ttgtcaaacc gatgctcgcc gttcaagcat
     5461 tatgggcttt tatgggacct tttggggatt tcatgttagc taagttcctg cttcgaacac
     5521 ctgaaaatca gactgttgct gtcggcttac aaacatttat ttctgatgtg acaaaccaaa
     5581 gagtctctct ttttgctgct ggggcaattt tgattgcact accgatttgt ctattattct
     5641 tctttttaca aaagaacttt gttaccggcc tgacatctgg cggaacaaaa ggataggagc
     5701 agagtatcat gcaaacattt ccgttaaact atttttccgc attacgtacg ccaactcaag
     5761 tgtttgcggg acgcaaacta ttatcatggc ctaaattttt cttgatattt gtatttttag
     5821 tttccttgat ggtcatgcct gtgactcttt tttatgccaa tcagatccaa gcgattccat
     5881 tagaacaatt tctatccgtt cattcgctga tcgatgaaca aggaacccaa aaattttctg
     5941 aacttgaatt atctgaaact gggttacaag catcgcaaca aacgatagct gtgacccctg
     6001 aaattttggt aggtgtgtct ttatctgaga aacaacaatc cgatcatgga acttttattg
     6061 attttgagaa ggaacaatgg gtgatccaac aaaaggataa atctggtata cgccgctata
     6121 cgatgaatta tagcccatct ttccaaccag attctgtacg tacgcccgaa gatttccaac
     6181 gatttttaga aagagaattt tatgccagta atcgtccaac aatcatttta tcttatagtt
     6241 taagtcttgg tcttgtccta tttgtcatga cgagtctcat tttattcggg gcttcctttt
     6301 tcttgtggat gacaagaaaa agccaacttt catccattca tacattcaaa gagagtgcaa
     6361 atttgatgct gaatgttatg ggaataggtt ccatggcagc agcagttgta gggtttatcc
     6421 actttgactt tatcttgatg ctaagtgtcc aaacaacgat aactgtcttg ctgcttttat
     6481 gggtttttgt caaaacaaga ttcaaagata agcgtgtcga atgacaagtt taagcgattt
     6541 atgcgacaat gaatgtgaaa aaaatgaaaa ataaagtagt ttttgaaaaa tttctgaaaa
     6601 aattaaactt cttagttgat tcttttcttt tttagtatta acgtaatagg gtagtttttt
     6661 taaggaggaa aatcatgtta gagaaaactt tatacaatca attattttca cgttctttct
     6721 cgctccctgt cgaagtaaat tattgggacg gtacaacgaa acaatacgga gatacggaca
     6781 ctcctcccca aatcaaaatc aatatccatg aagaaatccc aatgaaagag attacaagta
     6841 acgcatcttt ggcactagga gaagcctata tggatcatcg aatcgagatc gagggaagta
     6901 tccaagcatt gatcaacgat gcttatcagc aagcaaacag ctttttacga ggaaaagatt
     6961 atctaaaatg gctgccaaag aaagaaaaac atacgaaaca aaaaaacaaa gaagatatcc
     7021 atgcgcatta tgacttaggt aacgactttt ataaactttg gctagatcca actttgactt
     7081 attcttgtgc ttatttcaat acacctgaag atacgttgga acaagcacaa atcaataaag
     7141 tccatcatat cttagacaaa ctctttatca aagaaggcga aacgttgctt gatatcggtt
     7201 gtggctgggg aacattgatg ttcactgcta ccaaagagta taatgtcaag gcaactggga
     7261 tcacactaag tgaagaacaa tacgatttga ttaccaaaag aataaaagaa gaacatttag
     7321 aagataaatg ccgtgtatta ttaatggatt accgcgaact aaaaggcgaa acgtttgatc
     7381 atatcacaag tgtaggtatg ttcgaacatg taggttctga aaatcttgaa ggttatttca
     7441 aagtggtaaa agatttattg aaaccaaaag gaacagcttt gatccatgga atcagccgac
     7501 aacaaggcgg tgcgacaaat gcttggatca ataaatatat tttccctggc ggttatgtcc
     7561 ctggtgttac tgaattagtt gataatatta caaaaaacga tttgcaactg attgatttgg
     7621 aaagcttacg tcgtgactac caattgacat tagaacattg gacaaagaat tttcatcaag
     7681 tccaagatca agtcacgaaa gaaatgggtg attccttcta tcgaatgtgg gatctatatt
     7741 tacaagcttg tgcagcttca tttgaatcaa gtaatatcga tgtgattcaa tatttgcttg
     7801 ttcaacctgg aaacaatgac atcccaatgc atcgtgcata attgaggcaa aagtactgtt
     7861 taaagactat ttaaaaagaa gctaagtttt atgatgctcc agaagtgaac ttttttgtct
     7921 aacttctagg attgtctcta aagcttagtt tctttttctt tatattttca taataaaagt
     7981 ttggttcttt aa
//
LOCUS       DADVXU010000111         7875 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.116, whole genome shotgun sequence.
ACCESSION   DADVXU010000111 DADVXU010000000
VERSION     DADVXU010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.116"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..199)
                     /locus_tag="KB953_002470"
     CDS             complement(<1..199)
                     /locus_tag="KB953_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867400.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
     gene            complement(362..1996)
                     /locus_tag="KB953_002471"
     CDS             complement(362..1996)
                     /locus_tag="KB953_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBB6867401.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTSLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            complement(2000..3373)
                     /locus_tag="KB953_002472"
     CDS             complement(2000..3373)
                     /locus_tag="KB953_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBB6867402.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            complement(3389..3628)
                     /locus_tag="KB953_002473"
     CDS             complement(3389..3628)
                     /locus_tag="KB953_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867403.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            complement(3810..6485)
                     /locus_tag="KB953_002474"
     CDS             complement(3810..6485)
                     /locus_tag="KB953_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HBB6867404.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            complement(6668..7591)
                     /locus_tag="KB953_002475"
     CDS             complement(6668..7591)
                     /locus_tag="KB953_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867405.1"
                     /translation="MATNYAKKINFDVDKLYKSLIVVPKNKSEDGNYELEVLQFKIEQ
                     KSSERFMSNQLVKIKKWLPMIDQLVSNNKEAKNLCIFVLLLHGRVINLVNRKSTTKFA
                     NSFWFLKNINKTVEKNGVYKGDFHFQNAETIEREHHYFDLLVNTVVELAKWAKSNETY
                     SQVLKRIMSQETSFIYELSKSNLKYTYNEDELEILDNPENSQWIKELKREDFIETLRI
                     LKSLFDLKLAKATIMGIERDSYYWEEIGLYAPQIEGEDKEKRIDNIINYINSLSEPLK
                     CRGLDFIGTLGWTETYYEKIYKEIIEQYFPK"
BASE COUNT         2303 a         1557 c         1195 g         2820 t
ORIGIN      
        1 tataataaac atttccttga ggtttccttt taatcccctt cggaaaatca ccttttgtaa
       61 aatcgctctt aaggaaatca tctaaattac cagttctggt ctttttacct ccagtattcc
      121 ctgatttttt aggtggtaca tgttctttag taagttcagt gtatttttca cataaaatac
      181 acttacccat ctttttcata tctatcccca ctctttttaa ttcttatata caattatatt
      241 tcaaatattc attaataaat atccctattt atgataagga cctccctaac tacttctcta
      301 acgatttcaa atcgctagag aagcgttagc gcccctctaa cgttgtttga tataacctag
      361 actagatcgt taacgtaaag ggtgttttta acacaaaaat agccgtatcc tttgttttgg
      421 atacggctat ttgatattgc tcattcaatt gttgtaccac ggcatgcgtg aatttcatcg
      481 taatttccaa catctcctta tggttaggat caaaggttat tttctctata aaggtacgat
      541 acaaggcttt gatttgcttc ttctcactct gggcaagaaa acgatccaaa ctagtcaata
      601 tccgttggac atctttgact tgaatcggct ctccttgttg aaagatccct aagatttcat
      661 tttctcgaat gtgcaactgt cgtcgttttt cagtcaattc gtctaagcgg tcttttaaca
      721 tcggaaacaa ttcaggacta tcttccaaag cagcttccca tttttcaatt ttagtcttca
      781 attcttcctt tcgttccagt aacaccacga gttcttgttc caatggttgg ctttgtcgca
      841 ctatttcttc atttaacgct gccaccaatc ggggaagaac ttctggtagc tgaacgatct
      901 cctttaatcg gtcggcgaca aatgtttccg cttgttcggc acgaatcgaa ttggcatgac
      961 acacacttgc tcctttattc cgaaagactg aacaagaata atatcgaatc cgtttctttg
     1021 tgccatcctt tagggtattt gtgacattac tcgctgccat cggtgcgcca cattctggac
     1081 aacgtaacaa gcctgtcaat acattttcac ctgtatggtt ccatttgggt tgttttcctt
     1141 ctaaagcaag gcgttcttgt actttttgat aatcttcttc cgaaataatc ggttcatggg
     1201 ttcctttcac taaaatcggt ctcggattct tccccttccg tcgtttggtt tcccaattga
     1261 tgtagcgcgc atattctaaa taccctgcat aaattttatt gcgtaagata tctttgactg
     1321 ccgtggtaga aaaagcattc tttttgactg tttgatatcc ttgacgattc aaaaaattgg
     1381 caatcgctcg ataccctgct ccgcctaaat attttgcata gatcagacga acaattttcg
     1441 cctcttctgg ttcaatgacc aattgttttt gaccattcgt gtcaatacca ctgacatagc
     1501 ctaaacgtcg gcctcctgca taacgctttt tatctttcac taagctttgc attgacattt
     1561 gaacattctc cgcaatttgg ttgcgttcaa attcaccgaa actcccaagg agttgtaaca
     1621 tcaatttacc tgtcgaagtg gtcacgtcaa attgttccga gatcgaatgt aatcctacgt
     1681 catatcgcaa taattcctcg actgtttgta agacatctaa catattgcgt ccgagacggg
     1741 tcaatttcca aatcactagt agatcaaaac gatggttccg agcatcgttc atcattcgtt
     1801 gataggcgaa ccgatttttt gtggatttcc cactgattcc ttcatcggca taaacttcta
     1861 ccacttcata gcccatccgt tcggcaaact gtcgtccatt ttgttcttgt gcttgaatac
     1921 tatgtccttc taaagcttgt tcttgtgtac tcacgcgaat atacaatgcg actcgtttgg
     1981 tttgtatcat ttccatagat cattcgttcc tttctaaaat attttgtgtc agttgtcgga
     2041 agtaaatccc acaaactgtt ttatcctttg ttacctcaat ccgacaaaca tagtcacata
     2101 ataattgtcg actagagagg tcttttagtt gaagccattc tttcactttt gcttcacact
     2161 gttggagtga aagaaacgaa gaagtagaca tcggcttttg aagagcttgc agttgttgct
     2221 tgagttcttc cagtgaacaa ttgccctctt caaatgcctt aatccattgt gcttcttgtt
     2281 tcttttgttg gattactttg cgtttttcat cttttcgctt ttcggctaat tgttgcgtta
     2341 attgggcaat caaggcttga tagacgcctt cgtattgaaa aaaagcctgt atcttttcca
     2401 atacttctga ctcaaccctt tctgcccgaa gaaaaattcc ttggcagctt cggtcggtac
     2461 aatgataata atgaagcgtc ttcgtcttct ttgtattcca ttgatacttc gaactcagat
     2521 gacgaccaca gaagggacac cgaatttttt gacgtaataa gtacagatac gtatcttttt
     2581 tagctgtaca ccttgattgg cgaatctctt gggcttgtaa aaaggttgct ttcgagagaa
     2641 tcggttcaaa ggttcctaga tacttcccca aagatcctcc tttgatttcg ccatagtaca
     2701 atggattttt taggatacta taaatatgat agggctgaaa aggacgttcg cggaagaaaa
     2761 gcttttgttg cgtcaatcgt tgacaaagct ttttgtagcc atattttttg gtcaaataaa
     2821 gttgaaaaat atagcgaaca atccttgctt cgtgtacttc ttgttggatt ttcccattca
     2881 ctaaacgata cccatatggt accgcagaag acaaatattg ccgttgttgc tttttatagg
     2941 aaagtccggc acgaacattc tcaataatga cgtttcgttg ttgttctgca aaagtgaaca
     3001 ataattgcac aaatagttta ttcgttgacg cgtgtgagaa agaagcagga agaggttcag
     3061 aaatcgaata cacttgaacc tgatgttgtt ggcacaactc aataaattct aaggctattt
     3121 tggcttgacg tgccaatcga tcataacgcc aaacatagat caccccctct tcttctgacg
     3181 cacaaaccca agctctaagc tgtcgaaaag ctggacgctc cgctttttcc cctgattctc
     3241 ctttgtctga aaatcgttgg ggaatttgtt catatttctc taaaacatac tctttcactg
     3301 catcatattg agcgtcatta ctaattttcg cttcttcata aacagacgtt cgatcataac
     3361 aagccacacg catgcttgat tcccctctct atgaatgaac taaataccat tttttcacga
     3421 ctcttctatt gaacgaacgt tgcacaaagt gattttctaa atatccttcg ttcttcaacg
     3481 cttgaagagt acccccgata gatccagcag atacccctct tatttcgaaa tccaagtaaa
     3541 ttggttttgt aaaaaaggct tgatttgaaa aatgttcttg aatatacaga agaacctctg
     3601 cttttagtgt cattttattt ttagtcatta gaattttcct ttctttcatg tttgattgaa
     3661 tgaagtaaat gaagcataga tcaagcttca tttacttccg tttgggtttg ctcgctatca
     3721 tacagataag taatcagttg ttttgctaaa tcaataagaa atatttccat aacatggtgc
     3781 ctcactttca tactttggtt tattctaaat tacaatcgaa aatacgattt gagtgaagaa
     3841 ggtaatagaa tctcgtatgt tggaagaact tgacgattca tgtgaatacg tttcgttctt
     3901 ctcccttttt ctgcacacat attacccgaa tcaagcaatt gcctaaaaag ataggttaga
     3961 tcctttactt gaaattctcg ccttaacaag tcctcaaacg ggataggaaa aatatttacc
     4021 ttaatatccc catttttttg aattgatact tttccccaaa tcgtactggg cgtatgtttg
     4081 ttgtcactgt ttgttaaaaa tttatggcta ttcatcacca acaatgtttt cactcggtca
     4141 aataccgttt gctcacttcc taaactagta tctacaatta actgttcgta cgaattaata
     4201 agcaccgtct gtatatgctc taatcgtaaa ggatagccca gtatatcttg aattaggagg
     4261 gctccactat aaatcaaagc tagattatta ccaatgcgtt gcttcaaaga atgttcaggt
     4321 aaaacagccc atagtttttt ttgagcttcg acaaaagttg cttcaatttt tgtaatccct
     4381 tgttcaaaga gtttcaaaag aaataacttt ataccccaac caaaagtatt agaagaaaga
     4441 cttttaactt tctctgcgtg attcgcatcc tttgtaaact tgatatcaga aaattcgata
     4501 tatcgaactt tcaaaccagt taatttctca ttgaaataat tttccaaacg ctcttcagaa
     4561 gaggtaaggg tcaaaacaga aaaagtctct tgtggcataa gctgacaatt agaatctaat
     4621 cttagccttt cttctcctga agctaattga tagatcaacg agctaaagtt attttgagtt
     4681 gctgctccta actcatctag tattagtgga acacctccct gattgtttga tgctagcttt
     4741 ataatggctt tattggtagc attcatattt ctaagaaccg tagttggatt tccaaataaa
     4801 gaggcaataa agtgtaacgt agtggtttta cccgttgtgc tcttgccctg taggttaatt
     4861 agtaaactca aaaaagtatt agccttcgta acgtttaaat agcctaacac aatagaagca
     4921 ataccaatcg accaagcaag ttctaagttc gttcctaaaa catacttatg aaccaatgcg
     4981 tcaaattccg cttctgtacc attagttgtt aattggtagc atgagttttt agttaataca
     5041 gaaggaattt cacgtccaaa agaatcttgt aactgaaaaa tcattgcttc attttctttt
     5101 ttccagccaa gagattcatg ttcaaaaaag attggagcat tctcccgttg aatctgtaaa
     5161 aagtcaacga cttcagcttc tcttctagct ttaatgatta agccatgttt tgctaattgt
     5221 ttaaaattat tagtttgtaa acatccacct tctatttcta tttcatccca acaatgatcg
     5281 acataatagg caagaacaag tgtaatgact ttttgttcga tatttttgcg tatttgctta
     5341 ataaacatcg gacaagcaac tttttcttct tgaccttctt tcaccaaaaa tactccttct
     5401 tgattcagtt taaactggtc aaaataatat tcttgattgg attctaatga ttctacagaa
     5461 ggatttacag gttttggcaa cggatatttt tttacaaagt cttgaatatc tttattttta
     5521 tccgaagcat gcagacaatg aaatcgaatc ttattatcag ccgtaacaga caaacaagaa
     5581 ccattagaat tattcgtgtg ttttcttaat ggacattcgt caaatataag taaggtaact
     5641 cctttatgat cgccctcttt tatggtataa gttaattcag gatagtgagc taaccattgt
     5701 ttatagtctg cttgaataaa aggtaacact tctctaaaag atggatggct ttttactcta
     5761 gaggttgaca tcatggaatt ttcttcaact atctgttcaa gaattttaaa acaattactt
     5821 ctatctatgt tatcgggaat agaaaggaaa ctagataaac gatgcggacg ctcttttgtg
     5881 gactttcctt tgaccgctgg tgtaccaact aatttgcata atcttgaagg attataaaca
     5941 gaagtatcaa tatcaacttc atcttctact ccaaatcttc ggtgaagtaa ttgaagaaaa
     6001 ttctgaatag taagaatcgt ctcgtgatta tttggttgtt catttatggg aagaaaaata
     6061 tggtagccat tcccactatc aacttttaca aatgattcta ttctcttttc tttcaaataa
     6121 cgttctactt gttccataac attttctgcc tttttctttt cgctatctgt tgcacttccc
     6181 tctttccttt tagggtcaat atcaataaat acaaaccgaa ttttttcaat atcattgtct
     6241 ctcacagaat gctttttttg tgtaatcacc tcttgtttaa taggattata agttatgttt
     6301 actgtacctt cagacaattt ctgtttttct aaatagttaa taattgcttg agaatctgca
     6361 gtagaaaaaa tatgagaaac ggttttcttg tttaatgtag aaaaagttct caaattaaca
     6421 taagaaatcc ccagtgtatt taacagttgt aaatgcttct gaattgaagt gtgattcatt
     6481 ttcattaatt accactcact ttctaaaaaa tttttatttg tatgactaca ttacctccaa
     6541 cctctcataa tttcatgatc ttcagttcta caaaaatata tcccactcaa aatcttgaca
     6601 aaagaaaaaa gttgtgattt tggaaatcac aacttttttc ttttacgata aatcaatcct
     6661 tattaaatta tttaggaaaa tattgttcaa ttatttcttt atatattttc tcatagtatg
     6721 tttctgtcca tcctaatgtt cctataaaat caagtcctct acattttaaa ggttcactta
     6781 aactattaat atagttaata atgttgtcta tccttttctc tttatcttca ccttcaatct
     6841 ggggagcata tagtccaatt tcctcccaat aatatgaatc tctttcaatt cccatgatgg
     6901 tagctttggc taatttcaaa tcaaataacg attttagaat tcttagtgtc tcaataaagt
     6961 cttctctctt caactcttta atccattgtg aattttctgg attatctaaa atttcaagtt
     7021 catcttcatt ataagtatat ttcaaatttg acttgcttaa ctcatagata aatgaagttt
     7081 cttgactcat aattctttta agtacttgac tataagtttc gtttgacttt gcccatttcg
     7141 ctaattctac cactgtattc actaataaat cgaaataatg atgttctcgt tcaatagtct
     7201 cagcattttg aaaatgaaaa tcacctttat aaactccatt cttttccact gttttattaa
     7261 tattttttaa aaaccagaat gagtttgcaa atttagtcgt actttttcga ttaactaagt
     7321 taataactct tccatgtaat aataagacaa aaatacataa atttttagct tccttattat
     7381 tagatactaa ttgatcaatc atagggagcc attttttgat tttcactaat tgattgctca
     7441 taaatctttc actggacttt tgttcaatct taaattgcaa aacttctaac tcataattcc
     7501 catcttctga tttatttttg ggaacaacta tcaaactttt ataaagttta tccacatcaa
     7561 aatttatctt ttttgcataa tttgtagcca ttaatacatc tccttattca taataaattt
     7621 catatgacgc tcctctcaaa atttaccggt agactagaaa aaagaataaa agatacgtca
     7681 attcacactt tattcttaaa aaaaaactgg tctctctacc ggtaatatcg attgagtccg
     7741 cctttatcga ctttatcctt tttttatatt aaattttttc accggtagac agggagagaa
     7801 agaataaact aaaaagccct taattcttaa tgttttcacc tccttaccgt tcctaccgct
     7861 aaaatttaat ataac
//
LOCUS       DADVXU010000112         7811 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.117, whole genome shotgun sequence.
ACCESSION   DADVXU010000112 DADVXU010000000
VERSION     DADVXU010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.117"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(31..1383)
                     /gene="celB"
                     /locus_tag="KB953_002476"
     CDS             complement(31..1383)
                     /gene="celB"
                     /locus_tag="KB953_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBB6867406.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(1383..1901)
                     /locus_tag="KB953_002477"
     CDS             complement(1383..1901)
                     /locus_tag="KB953_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867407.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(1917..2243)
                     /locus_tag="KB953_002478"
     CDS             complement(1917..2243)
                     /locus_tag="KB953_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HBB6867408.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(2256..4235)
                     /locus_tag="KB953_002479"
     CDS             complement(2256..4235)
                     /locus_tag="KB953_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBB6867409.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(4256..4570)
                     /locus_tag="KB953_002480"
     CDS             complement(4256..4570)
                     /locus_tag="KB953_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HBB6867410.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4738..5031
                     /locus_tag="KB953_002481"
     CDS             4738..5031
                     /locus_tag="KB953_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBB6867411.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(5109..6221)
                     /locus_tag="KB953_002482"
     CDS             complement(5109..6221)
                     /locus_tag="KB953_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBB6867412.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            6422..6598
                     /locus_tag="KB953_002483"
     CDS             6422..6598
                     /locus_tag="KB953_002483"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867413.1"
                     /translation="MITEVSYETLQKKKTLIVAAVLFPAPCVCRNCGSAVVDGNRKLM
                     VVKNKKKETIVHFE"
     gene            6608..6787
                     /locus_tag="KB953_002484"
     CDS             6608..6787
                     /locus_tag="KB953_002484"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867414.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            6981..7151
                     /locus_tag="KB953_002485"
     CDS             6981..7151
                     /locus_tag="KB953_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBB6867415.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(7575..>7811)
                     /locus_tag="KB953_002486"
     CDS             complement(7575..>7811)
                     /locus_tag="KB953_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBB6867416.1"
                     /translation="DATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKH
                     LPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         2495 a         1554 c         1262 g         2500 t
ORIGIN      
        1 tttttgtcac agttcctttt ttcttagaaa tcattttaac ttttcactag ctaattctgc
       61 tgccttttct ctttcttcat ttaataattt attatcatag aatttgataa atgggaacca
      121 gataatgaat gcgatgattg cacaaatgac tgctaacaca gcagctttcc aatcaccacc
      181 actaccaaca aatgctccga tcccgactgg tgaaggccac ggcatctgtg caagcatcgg
      241 tttgacgatt tccagccgaa tggcaaaata agcgagtgta gcagaagcca tcggagctaa
      301 gaaaaacgga accgctaaat agggattgta gacgattggc atcccgaaaa tgattggttc
      361 attgatattg aataatcctg gtacaagtga ggcttttcct aacactttca gctgatcaga
      421 tttcgcgagg aatgcaataa agatgatcaa tcctaaagtc gcacctgatc caccaacggt
      481 tacataagaa ttgttgaatt ctcctgccaa cggataattt gcgccttgtg cgttagctgc
      541 catattagct aatacgatcg gggtcaagaa agaagtgatg atattcgctc catggatccc
      601 tacgatccac aatgcatgga tcaagaaata aatcaccatg acaccaagcc atgtattcgt
      661 caactgcgta acaaacccaa atgggatagc aatcattttg aagatatccg ttcctagtgc
      721 aactaataat ccattgatga tcatcaccac aaacgcaatg acaaatgttg ggatcagagc
      781 agtgaaagaa cgagaaactc ctggcggaac aacatctggc atttttatga cccaatttct
      841 cttgacacac atacaataca attcgaccgc aataatcgac ataataatag ctgtaaagat
      901 tcctgaagtt cctaaacgtt cgacaaaaga gcccatccta ataccattta ctacagtttc
      961 tgtctcattg atagaagcaa tcaagctgat tttaccatca gtcatgatca attcagggat
     1021 acacataaag aatgcaaaaa cagatagtaa tgcccccgtg agcggattaa catttaattt
     1081 ttgttcatct cgttcaattg acgttaattc ataagcaaac acaatggcaa agtaaataga
     1141 taaaatcccc atcgtcattg tattgacgat catgtacaag tcactaactt tgaaaaaagt
     1201 tgcattaaaa aatccttcta aaaatgggaa cggtaatggc aaaatattta aaaccaaaaa
     1261 catcgagcct acaatcgtga aaggaataga agccatcccc gctgccatga cagcccgaac
     1321 gattttaagt tgcgcaattt tccccatagg tcccatcagg tatttctgca aaaaatcaaa
     1381 cattagttat tctcctttcc taaatataac gctttttcat attgtttaat acggatatac
     1441 tgttggtctt ggttgagtga aattttcttt aaacgatact gtataaatgc actggcaaaa
     1501 ataccgctta caacaagaat gaaaacaaat aactgtcctc cattgttatt tgccataaaa
     1561 ggatacaaga atgtaaatcc tgaggtaaac aacactggaa tcactaataa attaaagaca
     1621 ctctggaaca aaaaatacca ctttgtccat ggaacgttcg tttggtgttt tcgataaagc
     1681 tttacttgtt ctcctactgc aggcaaaata gctaaaatca gaatcatcgg taaactactt
     1741 cctaaacttt tagctgatag aagcagaatc atccattgca tgttcatgaa aaagaaaaag
     1801 gcagtcaggt aacgtacgat tagataacga ttgaaataca tgtttttcaa actaagttgc
     1861 tttttttcta attcgatttt cctttttact gtatcctgca caatgatcct tcctttctat
     1921 ttattttttt gatacaaata attcatttcc tgtgcaactt ccaataatgt catcgtcgtc
     1981 atcagatgat cttgtgcatg gaccatgata atttccattt ctatttttgt cccgctggca
     2041 tattctttca gaagttgagt ttgtgactga tgcgcatgaa gaatctcttc atttgcttgt
     2101 cgcaacttct cttctacttc ttcaaacatc tctttacgca tcgagcgaaa tgcttcatgt
     2161 atcagtgtcc gtgcatttcc gctatggaaa atgatatcaa aagaagtcac ttgtagtgtt
     2221 tcactgttta ttttttcagc catctgtttt cctccttatt cgatcagttt gataaattta
     2281 ctgctgaaat tctcaaaaat aggttcagct aagatttcct gctgtacttt tagctggtca
     2341 actaacctaa cgattgcttt agtcacgacc gtaattcctt cattttcgac ataagaaggc
     2401 gaaactaaaa aaacaaaatg aatttcttga tattgatccc actgcatccc agaaggaatc
     2461 aatgcaacgg ctacctttgt ttcgattcca actggaacag cgggatgtgg gactgccact
     2521 gtatcactga atatgatttg tcccatattt tcacgttgct ggatctgctt tcgcatttct
     2581 cttatatatc cttcttcttc atttatgctc agcttttgca gcaaatcatg aagaatttct
     2641 tcctttttcg gttcgtacgt ataagtcttg aaaaattttt cagacaactg ttcggtgtaa
     2701 atctgctttt tttgctgtag agaaaatgct ggtatcggtc tatctgagga atgatagggt
     2761 atagattctc caactacctt tcgtatttta cgaacatcat cttcattcaa aaaaatactg
     2821 acatgtaaaa ctggaatttt gaaaaacatt gtcgaaagat caatagaaga aataatcagg
     2881 tcgattcctt ttaaagttgt ttcattgatc tcataatagc cttttacact taccactgta
     2941 atatttttac cgaattcact gactacgcga ttctttaata gttgggcact gccgtaaccc
     3001 gttgcacaga taatcaatgc atgtagttta tgcgcagctc tttctttttc caatgccgcc
     3061 atgagatgca aagccaaata ggcccattca tcttcattga tggtatagtt tttcaaagcc
     3121 ggcatgttgc tgaaatgcct ttttactgcg tcaaagactt tgggattttc tttctttatc
     3181 tcctcagtca atgggttctc aatgcgaata cctcggctca atcgaatcat catcggtgac
     3241 aaatgatcga gtagtccatt gaaaagctgg agatcctcta ctaatgaaaa atgaaattcc
     3301 tgagacaatc ttttcagaac tttcgctaat tcctttgata gttcctgatc attttgattt
     3361 tctttgtgat tcgacttagc catgagatgt aatgtcaaat aagcaatttc ttcttcggga
     3421 aacgatacac ctgcgattac ctcaatacgt tgaacgatcc gctgcgccac ttgatactct
     3481 actcgcttag aaacttcttg ttcgatgcct aattccttga tttcaaaccc ttctcggagc
     3541 cgtttgatcc ccaaagccaa gtgtaaagtc agattctgta tgataaaatc cgaaactttt
     3601 aacttagctt ctcttgtttc atcgagaatg ataatgacta gctcttcaaa actgatgtct
     3661 gggaaaaaac ggctttgccc taaatattct ttcaatgaat ttccataatg gtttccaaaa
     3721 aaagtatcca tgataaaatg ccgtcgattt ctttcttccc cttcgatcca tgtcccttga
     3781 ccatgcttag aaacgatttt tagtgaatat ggaatcaatc gctccttgat ttcttgcatg
     3841 tcctttgtca aactggaacg actgatataa agctcttctg ccaaatcatc cataaaaaga
     3901 atggcatctt ccaaaagcaa ttggttcaat atatactttt gtcgatcttc ggactcataa
     3961 aatccttgcg gttctctagc atatccaggt actatttctt gccgactcaa aaacaagtca
     4021 aatgtcagtc tttgtaaaat atgcagttgg tagccataac ctggtttagc aatgatctta
     4081 cctccgtttt tttctactaa ctctttgatc cgctgcaagt aattacggat tgtccgatca
     4141 gataataaca attcagaagc taaacgttga ctggtgacat actccttctt gtgatcaatc
     4201 aaatatctca acagcaattt ttccttctgt ttcattatca tccactccta ttccatcata
     4261 tgttctcttc gatcacctct gctaacttct ggatgcctgt agggattggg acatacgctt
     4321 ggaaagggat actaacaaca ggctttccaa cagattctcc taattttttg aatttatcca
     4381 aaaacatcgt cgtttgcggg ctgatcagaa ataaatcaaa atcactattc ttaatcatat
     4441 tatcgccttc tgtagctgaa actgcatcta cgagtacctc ttctcttttt tgcttaaaga
     4501 aatccgttgt ctttgaggcc atcatcgagg atgacattcc tgccgcacaa ataatcaatg
     4561 cttttttcat aaatcaatca ctccttttat ttttataaac caagcatatc ttgtaagcgt
     4621 tttctattca aaatatatat ttccgtagca ttgcggaaaa aatgtgtatc atatcttgaa
     4681 taataatttc tggcaccgtg atatactagt gatagaattt tttcaggagg ggatttagtg
     4741 agtatcacag tgaatatttt gtatacaagt acaaatggac aagccagaaa atttgcagaa
     4801 gaaatggtcg aaagaaacat tgtcaaagcg attcgtgcag aagaaggaaa cgagaaatat
     4861 gactattact ttccactaga agatccagaa tctcttttac tgatcgaccg ttggaaagat
     4921 gaagaagcaa tcgaaaagca tcacaaatcc gaaatgatgg ctcaaataaa cgaactccgt
     4981 aaaaagtata aattaaaaat gaaagtcgaa cgtttcactg atttaccgta aaaagataca
     5041 catggaatga tagcaattta tcatccatgt gtatcttcac tttttcctta ttttttattc
     5101 gatctcatct aatgattcaa accgtgtttt tctcttattt ttcggataag tatttcagtc
     5161 accccaatat acatgattcc taaacatacc ccaaggatat tctcaaaaat gcggatcgtt
     5221 actgctcctg gaaccccgaa taaactggca gtgattgcca aagcaccaaa agaattaaaa
     5281 actgttttat atctataggt agaacaaaca cctaaagcta gaccacctaa tataccgacc
     5341 cagttaaaag gaataaattg gctgatacca ataaataaaa cacatccaat caatgagcca
     5401 atgattcggt ctactgctcg ttctttgaaa ccaatcgaca tcaatccata gctagataca
     5461 atagaagaaa atgcaaaccc tgcccacatc aatcgctgaa aagggacata ctctcctgca
     5521 acgatcaaaa gactaatgcc aaatgcatag atgagcatcc aaattttttc ctgcgaatac
     5581 atgcctttca aaaaaatctc tttaaacaag cttttgtctt tgttcttttc tcgatgtttt
     5641 ctgtaaagga gaactgaaaa ccaacaaaaa aacaaaaata ataagctact tgtttgtgta
     5701 aagaaattct tgttcaacga atctttaggt aacgaataaa caataaagag atatgaaaag
     5761 ccgtacaaag aagcattccc cattttggga tcactagcgg ttgccattaa tatgctgctt
     5821 agtaaaataa aatgaatcaa catgcccata actgaccatt tgatcagctg gacatacggt
     5881 gctactaata gtgagaacat gacgattgct aaacctatga tcgaatggga aatcttataa
     5941 ccaaagtcaa caaaacgaat actcatcaac atacagaata aaaccacagc gtatggttta
     6001 tgcttcgatt caaataatgt tgtaattata atgataaaac aaatggcaaa caatacgatg
     6061 aataatgatc gtaaaaacaa tgaacaaata tacatgcact tttctttttt agaagctgct
     6121 tctcgtattt tttgttttaa aataaacgga tctagttgta gagactgata aaaatccacc
     6181 tgttgtcgtc ctttcttttt acttttattt tttacagtca tctatgacag gttgtaaata
     6241 ctatggaaca tcatatggcg ttgtcatttt cttgtcaact atttttccta ttcatgtttt
     6301 tataaaaaaa catatcgttt tcttgtatga ttaaatcacc acaaaataaa caactgagag
     6361 aacgatatgc tttatgaatg gttctatcaa aaaaattctg agatcaatag acaaaaattt
     6421 gatgattaca gaggtctctt acgaaacctt acagaagaaa aaaacattga tcgttgctgc
     6481 agttctcttc cctgctccat gtgtctgtag aaattgtggt tctgctgtgg tagatggaaa
     6541 taggaaatta atggtggtga aaaacaagaa gaaggaaacc attgtccact ttgaataata
     6601 taatcatatg ccaatggtta tacgcctaaa aaagcagcgc tatacctgta aaaactgtcg
     6661 aagccattgg aacgctcaaa gttattttat ccgtccaagg cactcgattt caaatcatgt
     6721 cagacataaa atcacttctt tactgactga aaaagtgtcg ttatttttta tttcgaaaag
     6781 ttgttaggta tctttgacaa ccgttattcg cacattgaaa gagttgagaa gctatttacc
     6841 aaagtcttcc aaaagaattc tgtcttgagt gttgagggtt gtgaatttcg ttctcacgtt
     6901 tccgttgagg ataaactgag ttttatttcc gcggatggcg agacagaaaa attaatcgag
     6961 aaaatttacc tacacaaaaa attcctcgat taacaaacta tttccttagc tgcactaatc
     7021 ctaaagaaat agaattctta gttacagaca tgaacgccac ctactttcag ctcaccaaac
     7081 atgttctacc aaatgcgaaa ttggtgattg atcgattact tggtttttcc acgcctttca
     7141 aggaggcgta acctttattc acgagttggt tgaagctttc cgagacaaaa gacccgactt
     7201 gtttttctcc ttattgacaa agagccatta aaactagaga aaaaattaca actgatcgct
     7261 gattatttgt agaaagaaaa tcaaagtatt acccaataaa cttgagtcat taggcaaaaa
     7321 acactgcgaa actcaagaat gattttcgca gtgtttttgt atatttttta ttaactagta
     7381 ttactacaaa ggtttatttt tcacttctca acgcagcaca aaattgtctt tacttattta
     7441 ctggttgggt tcaaccttct ctactccgat actaatattt gtttgtatac cccttctaaa
     7501 atcggtagtt tccattcaat tgatgggtac tagttgattt taaaaactat gcttattcaa
     7561 ttttttattt tagctcatat atttcgtttc tttctaaatt ttatactgat gaaaacaagt
     7621 agcacaccca atattgacaa tataaactgg ctaatgatgg tttctccagt ttttggaaga
     7681 tgcttatttc cactgcttgt tggattgttt cctcttatat ctgagctatc agcaactttt
     7741 ctattctctt ttgttaaaaa gatcgccact tcttgttctt tttcgatctt gaaagttact
     7801 ggtgtcgcat c
//
LOCUS       DADVXU010000113         7738 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.118, whole genome shotgun sequence.
ACCESSION   DADVXU010000113 DADVXU010000000
VERSION     DADVXU010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.118"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..107
                     /locus_tag="KB953_002487"
     CDS             <1..107
                     /locus_tag="KB953_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HBB6867417.1"
                     /translation="NESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(176..904)
                     /locus_tag="KB953_002488"
     CDS             complement(176..904)
                     /locus_tag="KB953_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBB6867418.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(917..2197)
                     /locus_tag="KB953_002489"
     CDS             complement(917..2197)
                     /locus_tag="KB953_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBB6867419.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2338..2940)
                     /locus_tag="KB953_002490"
     CDS             complement(2338..2940)
                     /locus_tag="KB953_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBB6867420.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3185..3391
                     /locus_tag="KB953_002491"
     CDS             3185..3391
                     /locus_tag="KB953_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867421.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3703..4422
                     /locus_tag="KB953_002492"
     CDS             3703..4422
                     /locus_tag="KB953_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBB6867422.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(4503..5186)
                     /locus_tag="KB953_002493"
     CDS             complement(4503..5186)
                     /locus_tag="KB953_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HBB6867423.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5405..7591
                     /locus_tag="KB953_002494"
     CDS             5405..7591
                     /locus_tag="KB953_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBB6867424.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2776 a         1302 c         1463 g         2197 t
ORIGIN      
        1 gaaatgaaag tcaagaagta ttgctgaaag aaatggagaa ttggccaaca ttttatccag
       61 atacaatgga tggacgtgaa attgcacagg aaatgctttt gcactgatag gcaaaaaaga
      121 accgaagggt ttggatcggc tggtcattta gtcggtccaa actattcggt tctttttatg
      181 aaatcaattt ctttaatcca cggatagagg ctgggatggt ctcgccgttt gccaacgtga
      241 cttctctggt ttttttatta atcgaccgca cattttgtgt attgataaca taggatttat
      301 gacaacggat aaaattccga tgcattttct cgatttcttt gatcttctca tagaattgta
      361 attgggcatg agtcaaatgg acttccagtt tatgtggcgc tcctagcgaa gtagaaaaat
      421 attggatgtc tttgatcggt aaaaatttga tctcatcatt aaactctatt ttgaagatat
      481 ctttttggat ttgggacatt tgaaaatctt cgaaaatttt gctcaggata tgctcgacat
      541 tttcttttaa atcataaaga tcttctttga cgatataatc agtcggttcg atgttgcttt
      601 ttaagatttt catcgacatt tccgtatggg aagtcacata gattattttt cctaaaggat
      661 ctaattcacg gatcaattta ccgatcgcta caccatccat ttcactgtct tctagttcga
      721 tatctaaaaa gtaaatacca aaagaagaag ttctgatctt ggctgcattg agaagttctt
      781 ccggatcaga ggtagaaata tggacataac tgtctaagtt ttcaatcatg attctatttt
      841 tgatcaccgt tgataaaact tctaattgtt ttggatcgtc ttcacaaata taaatgggaa
      901 tcatatcatt actcctttat tttatttgta tataaatcgt ttgagaaaaa cgctggtttt
      961 caattgatgt ttcaaggaaa agataaggat tggtctttgt caattcatcc aagataaaca
     1021 agccaaggcc ttcgtttttt ggatttgttt ttgttgagaa ccctttttgt ttcaactggt
     1081 ttaacggtaa tgtatttgtt gttgtattga tcacacgaat catcacggaa tgattcttct
     1141 tgagtatggt caattcgatt tttttcttct ttgcagcact agctccttca atcgcattgt
     1201 ccaagataat accaagcata cgtacaaggt ttaccgtatg tttcgtatcc agttcgattg
     1261 gctcaggaaa atgaagctgg acgatcaacc ctttttcctg tgccatcatg agttttaatg
     1321 acagtaaact acggatctca ggaactttca tattatccaa catacttaga ttggccggaa
     1381 aaaggttcat catctggcgt gtaggtaata tcgtttgatc ataataagac cgtaatcctt
     1441 ctaagtcgcc ttctgaaata tagctgtgaa gagaaaaaag cagattttgg taatcatgtt
     1501 tgaattctct caagtcttga tagttttttt ctagatcttt gatataatcc tgttgcatcc
     1561 tgacttgttg ctctaataac tgatcagcta atttcttatt agagattttc caataataaa
     1621 ccaagatgat caggccaaca cacagatata gaatgaaaaa actcgtattc aaagctaaga
     1681 catcaggtgt ttcaccagaa aaacgggtat aaaaaataat gatatagtat gtaaataata
     1741 tcaatgtccc gatcgtaccg atcatacggc ggtccacagt gtgaaaccaa gtctctaacg
     1801 atttttttaa acaaaaagct aataaagcag ataaaaaagc ggataagaca atgtgaaaat
     1861 actgcaatga atctcctggt tcaataaccg aagatttcaa aacaaaaaaa tcgccgatgc
     1921 ttgttaagtg atctcctaaa agcgacgtca ataacccaaa aggaagcgcg taaaccaatt
     1981 gttgacgcga agtcgaaaag aagaaaaaag taagcatcag cattcctata gcaattagag
     2041 cagtaaaaat ccctaaaatc gaatagaccc atccaatcaa aatacaagaa atcagaatga
     2101 ctcccgcaaa cagatatttg tatcggatgt gatggtcaat caataagata caaattgtgt
     2161 aaatcaaata ttgcaacgct atggaccata aaaacataaa gtacagctcc cattgatttt
     2221 taccaaatta tagcacaatt ggcgtattat ttttttaatt tttcagggat tttgaattca
     2281 gaactcatac caaagcagca gccagataca aaaaagtcag ataagttagt taattgttta
     2341 atgattgttt ttttcatgaa agattcctcc aaaattcatc aagtagtttg taataggaag
     2401 caacgtagct atttctaaaa caatcccaag tagcaaatag gtagaaaagt aagaagctga
     2461 gaaaaataac gtaatgagag aaaaaattag aaacatgctg atcgccttct ttaataatgg
     2521 gggacgtttc tcgggttcaa tgggattgat tgctgtacct cttgatccat agcgaagcag
     2581 taacaaaaag cagctgatta acaaaaagac catcattggg aaggagatag tcaattgcag
     2641 atgtttcatc accaagggaa cgccccataa ataaagtagt gtccacagca gacaaacgaa
     2701 ttcagagtcg gaatgcaatc cataagcata tctgcggatg cagatataag aaagaaaggt
     2761 gattcctgtt tccgcccatg agcctgtcac cagagaaaag actagaacga ccgctagttt
     2821 tccaagatta ttcaataaaa cttctaagac aaattttact tggacataca ccatatcttc
     2881 ttccgatttg tctccgctga ataaggcttc taataatttg ttcacaactt tatctttcat
     2941 ctcatacgta cctccaccac aaattttatg ctaaaaaaaa taaaaaacgt cttttgtttc
     3001 ataaaaagtg gttttgacat caaaaccgtc atgatctcct aaaaaaatct tcgttttcca
     3061 agaaccttac caaaaaaagt gacagttttg atacgaaaag tactttttat gacaagtttg
     3121 tgctttttcg agtgatttat gttaaaaaat tactgacctt ataaaaggtg atggagggat
     3181 tcttatggaa aatgaacaat tgtttgaagt cggcgaaaca tatgcattta aatctgattt
     3241 atttgaatca ccaataaaag gaaaaataga aaaaatctat gaaaatagtg tgatgatacg
     3301 tgtcatgaaa tgcgcagtag aagacaatga taccgctcat aatctgcact ggcagttagt
     3361 tgtacgcaaa tcaagtacaa tagaagaata aaaacagata aaatgttgag agcataaaga
     3421 ggttgtgagt ctcccgttta gctctgagtt acaaggaata atgttaaaaa acagcttttc
     3481 atgttttatg aatattgcct tgtgaccgag gtgttggcag gagaatactg tttatttgaa
     3541 aaaagggctg cgacaagact tttgtcacag cctttatttt catgaatagg agttactttt
     3601 ggctactttt agagaagtag cttttaagga gcagataaag tcaaggcttt tatttctata
     3661 aatgaagtag taaaatgaag aacagatttt attacaggaa ggatgccgat aattgtgatc
     3721 attgttgcag gaacgatcgg tgcagggaaa agcacactaa ctgaaatgct tgcccaagat
     3781 ttagaaacaa aaccttttta tgagaacgtg gaagataatg aagtattgcc gctattttat
     3841 tctaatccag aaaagtatac ttttttatta cagattttct ttttgaacaa acgctttttg
     3901 gcaatcaaag atgctttcag ccatgatgac aacgtactag accgctccat ctacgaagat
     3961 agtatgctgt ttcatttgaa tgcagattta gggcgtgttt ctgaagtaga agtaaagcag
     4021 tatgaaggtc ttttagaaac gatgctgaag gagttagaag aaatttcgcc acaaaagaaa
     4081 ccagatctac tggtttatat tcgtgtatcc tttgaaacta tgttagcgag aatcaaaaaa
     4141 agaggccggg aatatgaaca gctagaacaa gatcctgaac tctattccta ctataaagaa
     4201 ctcaatcgac gatacgaaga gtggtatgaa cagtttgata tatgtcccaa aatagtgatc
     4261 gatggggaca aatatgattt cgtagccgat ccggcatgtg gagaacaaat cgtacaagaa
     4321 atcaagatgc gggcaaagaa aatgacagaa gaggcagatg cccagtcgat ccgctacact
     4381 acaaataacc cgaaagcaaa gggaatcttt acaactaaat aatataaaaa ctaagcaaat
     4441 tgctcactcg cagatatttc tctgtgagca gcatcttgct tagttttttt gattcattga
     4501 gatcaaatag tgaaccgatt cgtatgataa aattgccata aatgaaatgt ctgagtgagg
     4561 gccatgatca aaaataaaat ggaaacgatc aaaatcgaat gggcgccaaa cagatgatga
     4621 cagaaaacaa ggacaatagc tcctgcgata aaggcaagaa cgacacttag atagtggaaa
     4681 aatcgatcaa aatctgcttt ttctttagaa atacaataat catataagtt cgttgctacc
     4741 ccttttaaat ttccagttgt aaataaatta gtgtaggcac gcccgttgat tttatcaaaa
     4801 gctacccact ggatcgatgc agtaaaagca atcaaaatcg ttactaatgc gggatgctca
     4861 tggacgggtg tcaaactaac aaagaaaaaa atcactgctt cataatatag aacaaacaag
     4921 cgccagaaat gtttttcttt ttttctaaaa taatcaatta gatactttgt tagaagaatc
     4981 cctaagaaaa agaaaagggt agacaatact tttttaccaa cacttagcca ttctccgtca
     5041 aatgcttgga tgatcgatag aatgatattg ccagtttgag cagaggcaaa agcaccatat
     5101 tggatataag tataactgtc catggctcct ccgataaatg tcagcaaaag tcctattgta
     5161 cgcccctcgt gaaaggaagt ctgcattttt ttcatcacct tttatgaata tttttgtctt
     5221 ccttatctta gcataaacga ttgaaaaaaa aggaaaaatt ttcagaaaac taaaatcata
     5281 tcgcttccca ttttcatttt tagaaaggtc tgttagtata aagaaaaaat agacaacgaa
     5341 actttaaata accaacttga aataaacaaa ttcaaagaag aaagatggga aggaaatcaa
     5401 tcagttgaat gaaaaagaaa ttctccgatt acaaaaagaa ttgaaacgct gggagaacaa
     5461 gtataaaaag cttcaacaag tctttcggct acaagaagaa atcattgcta cctatgaagc
     5521 ggcttatggc gaagcacccg gacttattga ggaagaagta caagtaaaag aaagtgaagg
     5581 aaacagctct ttctttaaaa tgatggagaa acgttcctat aatcgcttca atcaagaaca
     5641 aaaagcagct atcacatatg atatgaagaa acatcttcga atcatagcag gagctgggag
     5701 cggcaaaacg caaacaatct gtgcaaaagc tgtttatctg atgacgcaaa aacaagtaga
     5761 cgaagaacgc atactaatga tcacttttac aaggaacgct gcaaatgaac taaaaaaacg
     5821 agtagataat ttcagccaga gaaaaaccgc tgtccatatc ggaacattcc atagtatctt
     5881 ttttaggata tacaatgaaa tttgtcgaaa atttcctgaa gtagcaatgc aaggaataca
     5941 aggagacttt tcaaaggata ctgcacaaaa agtgaatgct gttttgcaac agctgatacg
     6001 taaatataat ctttatatat ttgatcaata tggagaaaaa acgattgctt ctcggttaga
     6061 ctattggcag aatatgaacc tttcagtaga agaaatggtt cagttagtta aagaaaaata
     6121 tgattccatt gataagaatg cacgacagcc gatatcagaa cgactgtatg gattgctcac
     6181 ggaactccaa gaacaaaaaa gaagccagca attgctagag ttcaatgatg tactccaaaa
     6241 tctgaaaagc gctttggaga atgaggagat ccagcattat atcggccaaa aatatgatta
     6301 tctattcatt gatgaatttc aagatactaa tccacttcaa tgggaaatcg tgaaacggat
     6361 gacaaaggaa aacaggatca agttgattgt tgttggagat gatgatcaga gtatctatgg
     6421 attcagagga tcagagccag catacatcaa gaactttgaa aaagaatatc ctaccaaaac
     6481 tcttttctta ttaactaatt atcgttctcg ggcagctatt gtccaaggag caaatagatt
     6541 gatcagctat aataaaaatg atcggatacc aaaggcgatg attccagcac aaaaagaagc
     6601 aggtgttatg gaagcctatt tgttttctga tactaaagca gaaagtcagt ggctaatcaa
     6661 acaaattcag acatttattc aaaaaaatgg gaaatataag gaatcaatca tattataccg
     6721 atcagcttca caaacgcagc agcttgtcca atctctgctg aaaacgaata ttccttttgt
     6781 actggaagca gatagtccat atgaaggtat ctttggtatc aagtctttcc aacatttcta
     6841 tcaaaaaatt gttcattggc aaacagcact gaatctgcaa aagaaaaatc aggcctacca
     6901 gcaattgctc cggcagctga tggcagactg ctacctgaaa aaaagtgaat gcgatcaata
     6961 cttttctcct aaatatcaaa atatagccgt tgcagattat attttgtcga taagccccaa
     7021 tttaaaatcg aaatctgcag aaatccgaga gtttgaggaa gcgttgcgta aaacagacca
     7081 gcatcaagaa atctattcgc ttgttcaaag ctatcttcat ctgccacgta tagctaagga
     7141 aatcgaccag agtgatcaag aatggatacg agaagaagta aagcagcatg ctactttcag
     7201 aagactccaa gccttatcgc aagaaaccca agcgacagct aaagaattaa aaacccgctt
     7261 gattgcttac agacaaggaa aacttgatgc actatgtatc cagagcattc acaaaagtaa
     7321 aggtcttagt taccgaaatg tctttttgat tggttgtaac gaaggaagtt tgccttataa
     7381 cggagcgaca gaaaagaaag taatcgactt taatgaaata aaagcagaac cagtaacgac
     7441 gatagaagaa gaacgaagac tattttatgt tgcgatgact agagcaagaa atcgtctgta
     7501 tctatgtgta cctcaaatga aaaattcaag aaaattgaaa gtatcgagat ttatcaaaga
     7561 aaccgggagt cgtgtgaaaa aaggaaagtg atagaagaga gggtgtgaca gaagtggtca
     7621 gtttcaagaa agaagaagga atgtacgaaa attgcttctc aaatttttgt gaaatttcag
     7681 cttattttcg aagaagctat ttctggaaca cagtttattc ggaaaaggga ggtgtgac
//
LOCUS       DADVXU010000114         7601 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.119, whole genome shotgun sequence.
ACCESSION   DADVXU010000114 DADVXU010000000
VERSION     DADVXU010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.119"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(943..1356)
                     /locus_tag="KB953_002495"
     CDS             complement(943..1356)
                     /locus_tag="KB953_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HBB6867425.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="KB953_002496"
     CDS             complement(1812..2297)
                     /locus_tag="KB953_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867426.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="KB953_002497"
     CDS             complement(2436..2651)
                     /locus_tag="KB953_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HBB6867427.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2875..3675
                     /locus_tag="KB953_002498"
     CDS             2875..3675
                     /locus_tag="KB953_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBB6867428.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3694..5562
                     /locus_tag="KB953_002499"
     CDS             3694..5562
                     /locus_tag="KB953_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBB6867429.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5555..6046
                     /locus_tag="KB953_002500"
     CDS             5555..6046
                     /locus_tag="KB953_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HBB6867430.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6034..6588
                     /gene="srlA"
                     /locus_tag="KB953_002501"
     CDS             6034..6588
                     /gene="srlA"
                     /locus_tag="KB953_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBB6867431.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6606..7601
                     /locus_tag="KB953_002502"
     CDS             6606..7601
                     /locus_tag="KB953_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBB6867432.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2708 a         1213 c         1443 g         2237 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2701 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     2761 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     2821 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     2881 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     2941 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     3001 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     3061 gacatagaag aagggatata taaagtaatg aaacatttca agacgatcga tggtctagtc
     3121 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     3181 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     3241 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     3301 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     3361 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     3421 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     3481 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     3541 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     3601 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     3661 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     3721 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     3781 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     3841 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     3901 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     3961 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     4021 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     4081 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     4141 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     4201 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     4261 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     4321 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     4381 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     4441 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     4501 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     4561 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     4621 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     4681 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     4741 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     4801 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     4861 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     4921 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     4981 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     5041 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     5101 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     5161 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     5221 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     5281 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     5341 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     5401 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     5461 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     5521 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     5581 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     5641 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     5701 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     5761 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     5821 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     5881 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     5941 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     6001 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     6061 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     6121 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     6181 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     6241 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     6301 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     6361 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     6421 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     6481 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     6541 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     6601 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     6661 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     6721 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     6781 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     6841 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgtttccaca ggaaaaagtg
     6901 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     6961 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     7021 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     7081 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     7141 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     7201 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     7261 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     7321 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     7381 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     7441 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     7501 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     7561 ttatcattgc ttgggtagca agttttggat tatatagctg a
//
LOCUS       DADVXU010000115         7482 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.120, whole genome shotgun sequence.
ACCESSION   DADVXU010000115 DADVXU010000000
VERSION     DADVXU010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.120"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            82..549
                     /locus_tag="KB953_002503"
     CDS             82..549
                     /locus_tag="KB953_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HBB6867433.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            556..3048
                     /locus_tag="KB953_002504"
     CDS             556..3048
                     /locus_tag="KB953_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBB6867434.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            3269..4192
                     /locus_tag="KB953_002505"
     CDS             3269..4192
                     /locus_tag="KB953_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBB6867435.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            4198..5451
                     /locus_tag="KB953_002506"
     CDS             4198..5451
                     /locus_tag="KB953_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBB6867436.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            5585..6931
                     /gene="gor"
                     /locus_tag="KB953_002507"
     CDS             5585..6931
                     /gene="gor"
                     /locus_tag="KB953_002507"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HBB6867437.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGIGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
BASE COUNT         2600 a         1204 c         1628 g         2050 t
ORIGIN      
        1 ttaacaaaaa gcgttcttct ttctttctat tatcaaattc gttataatta aggtcaatat
       61 tagtcaaagg gaatgaataa gatggcacat cagaatacct cggatttaat tgaatcatat
      121 ataaagaaaa ttctggaaaa tagtgaaatg atcgagattc gaaggattga aatggcagac
      181 ctctttaact gtgttccttc acaaatcaac tatgtgatta atacccgttt taccatccaa
      241 cgtggttatc tagtagaaag caaacgtggt ggtggtggtt atatacgaat tgcaaaagta
      301 aggatttcag ataaaaaaca attacttgag cagataaatc agttatttga tgatacaatc
      361 agtgagaaaa atgcttttgc tattattcaa aaactttatg aagaccaaat aattacaaaa
      421 aaagaaggaa acttgatgtt gagtgcaatt gcaaaaaaca cattgaattt caatgaatat
      481 gaagatcata cacgtgctcg gattctccga gcatttctgg aacgtttaag ttatgaagat
      541 gggaagtgag attagatgga agaacttttt actgaaagag cccaagcagt attagaaatt
      601 gcacaagaag aagcaaaacg gttgaaacat caatcagtta gttcagaaca tgttttacta
      661 gccttagtgg tcgagcaaaa tggaattgct ggaaaagtat tacgtgaaat ggatttgaat
      721 gaaacggaaa tttatgaaga aattgaacat ctgattggtt atggtggtat ccgttcttac
      781 cctaaaggag tctttcttcc atattcacca agaatgaaac aagtttttgc tttagctagc
      841 agcgaagtga agaaaactgg ttctcaaaaa gttggaacag aacatatttt gatgagctta
      901 ttaaaagatg aaagtatcat ggctactcga atcatgatca acttaggtat cagcctttca
      961 aaagcccgtc aagttctgaa acaaaaaatg gggctagctg gtgattctgc tggaaaagga
     1021 atgaatcgtc gtagaaatgt aaatgtacaa gataaacggc aaacaccgca aggaacacct
     1081 acgctagatt cattagcacg tgatttgacg aaattagcaa aagaaaacaa gctggaccca
     1141 gttgttggtc gttcgagaga agtgaaacgg cttatccaga ttcttagccg acggacaaag
     1201 aataaccctg tactggtggg tgaacctggt gtaggcaaaa cagccattgc tgaagggtta
     1261 gctcaaaaaa tcattttagg tgaagtgcca gaagacatgc aagaaaagcg cctgatgatg
     1321 cttgatatgg gatctttggt tgctggtacg aaatatcgcg gtgaatttga agatcgaatg
     1381 aaaaaggtca tcgacgaaat ctataatgat ggtcaagtaa tcttattcat tgatgaattg
     1441 cataccttaa ttggtgcagg tggagcagaa ggagcaattg atgcttcgaa tatcttgaaa
     1501 ccggcgttag ctcgaggaga attacaaacg atcggagcaa cgaccctaga tgaatatcaa
     1561 aaatatatcg aaaaagattc tgcattagag cgacgttttg ctcgcattca agtagatgag
     1621 ccaactcctg aagaagcaga agtgattctt caaggattac gtacaagata tgaagagcac
     1681 catggagtgg agatcacaga tgaagctgta cgagcagcag tcaatctttc tgttcgttat
     1741 atcacttctc gtcaactacc cgataaagca attgatttga tcgatgaatc agcagcgaaa
     1801 gtccgtttgg atcaaaccga tcacttaaca aaatcaactg tgatcaaact agaaatcgat
     1861 gaactagtac aagaaaagga agcagcgatc caaaaacaag actttgaaaa tgctgctcaa
     1921 ttacgcagac aagaaaaagc actacgtaaa aagctgcaaa aagtttctgc aattgaagca
     1981 aaacaggagc aaggttattc tgatcgtgta acagaagaag atgttgctac agttgtatcg
     2041 gagtggactg gtgtccctct acagcagttg gagaaaaaag aaagcgaacg cttgttagaa
     2101 ctggaaggac ttctgcatga acgtgtcgtt ggtcaagatg aggctgtcaa agcagtttca
     2161 cgtgcgatcc gccgtgcacg aagcggattg aaagatcctg atcgtccaat tggttcattc
     2221 atgttccttg gaccaactgg tgtcggaaaa acagaattgg ccaaagcact ttcagaagtc
     2281 atgtttggaa gtgaagatgc cctgatccgt gttgatatgt cagaatttat ggaaaaatac
     2341 agtacaagcc gattaatcgg gtctcctcca ggatacgtag ggtatgatga aggtggacag
     2401 ttgacagaaa aaattcgtca aaaaccatat tcagtgattc tcttagatga agtggagaaa
     2461 gcacatccag acgttttcaa tttacttctg caagtattgg atgatggtca tttaacagat
     2521 tctaaaggta gaaaagtgga ctttagaaat acaatcatga ttatgacttc aaatatcgga
     2581 gcgacacaaa ttcgagaaga aaaaaatgtc ggattcaatg tccaagatgt tactaaagat
     2641 cacaaagcca tgcaaaaacg tatcttagaa gagttaaaga aagctttccg acctgaattc
     2701 ttgaatcgaa tcgatgagac tgtcgtcttc cattcattga gtaaagatga aatccataca
     2761 atcgtccaaa tcatgagcaa agcgattatc aaacgattga aagaacaaga tattcaagtg
     2821 aaaatcacac catctgcaat cgatgtgatc gggaaagctg gatttgatcc tgaatatggt
     2881 gcacgtccaa tccgtcgcgc acttcaaaaa gaaattgaag atcgattgag cgaggctttg
     2941 cttggcggag aaattcgttt aggtgaccac gtaacagtag gtgcttcaaa aggtaagatc
     3001 acattgaatg ttcgcggacc taaaaaggaa acagtaggtt ccctatagtt acttggcctg
     3061 tgtagctgaa tttttataat agtaagactt gattaagagg ttgtgacaag atttttgtca
     3121 caatctcttt ttatatttgg acagaatata tcgaacttta ttttgatttt ttgtgaaata
     3181 attttcaaaa atccgacttt aaatagaaga agtattggaa agtttttaga gctatgctac
     3241 tattatgaag ttgtaggagg agaaaattat gatcgaactc ataaccacag tagaaactat
     3301 tgaacaagca gaacagttgc ttgctgcagg tgcagataca ctatatttcg gtgaagagca
     3361 atttggattg cgtctgcctg cttcatttac acgagaagaa cagcgacaat tggtagaatt
     3421 agcacacaag tatggtaaaa aagcgaatat tgctgtaaat gggatcattc atcctgaaaa
     3481 aatgaaaaat gtacctgaat atttggcttt tttaaaagaa ataaacgtag atgtgatcac
     3541 agttggtgat acaggtatta tttatacgat gaaaaaaaat cctgaattgc gtataccgta
     3601 tatttatgat gctgaaacat tagtaacgag cgcacgtcaa atcaatttct gggcgaaaaa
     3661 aggcgcagtt ggagcagtac ttgctcgtga agttcctttt gaagagatga aggcgatgga
     3721 agaaaaactc gatattcctg tagaaaccct tgtgtatggt gctacatgca tccatcaatc
     3781 caaacgtcca ctgctacaga attactataa ctatacgaaa caagacgaac aaaaagatcg
     3841 tgaaagaggt ctttttatct cggaacctaa aaaagaagag acacattatt ctatttatga
     3901 agatagtcat ggcacacata tttttgccag caatgatctg aatctctcga atgaattgaa
     3961 tgaactttat cagcatcatt actgtacatg gaaattggac ggattattta ctccaggcga
     4021 aaaattcgta aacatcactc gactattcgc cgaagcaaaa aaacaaattt tagaaggtag
     4081 ctgggatcaa gaaaaagcgg aaaaaatgac tagcgaaatc agtaaattgc atcctgaaaa
     4141 ccgtgaatta gatacaggat tcttttatat tgatccttct gctatcaaat aaggagtatg
     4201 atgaaagaaa tgacagtaaa aagaaaattg aaacgaccag aaatcctagc gccagcagga
     4261 acactggaaa agttaaaaac cgcgatccat tatggagcag atgcagtata tattggcggg
     4321 aatgcatatg gtttaagaag ccgtgcaggt aattttactt atgaagaaat ggctgaagga
     4381 gttgcttttg caaaagaaca tgatgcgaag gtatacgttg ctgctaatat ggtcacccat
     4441 gaaggaaatg aagatggagc aggggaattt ttccgcagtt tgcgtgatgt cggtatttct
     4501 gcagtgattg tttctgatcc agctttgatc gaaatttgtg cgacagaagc cccaggtctg
     4561 ccgatccatt tatccactca agcttcagca actaattttg agacattgga attctggaaa
     4621 gaagaagggt tggaacgtgt cgttttagct cgtgaagttt caatggagga agtggcagag
     4681 attcgtaaaa atacggatac cgaaatcgaa gcatttatcc atggtgccat gtgtatctct
     4741 tattctggtc gatgcacatt gtccaaccat atgtcgatgc gggatgcaaa ccgtggcgga
     4801 tgttctcaat cttgtcgctg gaagtatgga ttgttcgata tgccttttgg tcaagaacga
     4861 aacagtttaa ctgatacagg cgaagtggaa gaagaattct cgatgagtgc agtggatatg
     4921 tcaatgatcg aacatattcc agaattgatt gaaaacggcg tagacagctt caagatcgaa
     4981 ggaagaatga aatcgatcca ttatgtatct acggtgtcaa atgtctataa agcggctgta
     5041 gacagttata tggaagatcc tgaaaactat gtctgcaagc aagaatggat cgacgagtta
     5101 tggaaagtag cacaacgaga attagctaca ggtttttact acaatacacc aactgaaaac
     5161 gaacaattat tcggtgaacg tcgtaaaatc ccagtatata aatttgtcgg tgaagtatta
     5221 tcttatgatg aggcaacaca gattgctacg atacgtcaga gaaacttgtt ttctgtagga
     5281 gatgaaatcg aattttatgg tccaggattc agtcatttca gtcaaaccgt tgaagttatg
     5341 cataatgaag aaggcgaatc cattgaccga gcaccaaatc caatgatgat tttaactatg
     5401 ccagtcgcac agccggtgaa acctggagat atgatccgaa aacgtaaata aggaaaaagc
     5461 tgatatatcc ctgttttaag catcttcgca tacaaaattc aagaaaagtg ttacacttgt
     5521 tttctgtaga aaaaggatcg ttaaataaag gttttgattg atctcttcaa taaggaggaa
     5581 caaaatgcaa atatatgatt acatcgtaat tggtggcggc tctggcggta ttgcttcagc
     5641 aaacagagca gggatgcatg gcgcaaaagt tttgctgatt gaagcgaatg aattgggcgg
     5701 aacatgtgtc aacgtcggtt gtgtacctaa aaaagttatg tggcaagcaa gtacgatttt
     5761 ggaaacaatt gaacgagatg cctcaagtta cggtatccaa gcagatttaa aaaatgttga
     5821 tttcgccggt ctagtagaaa gacgagaaaa atatattgat tttctacacg gtgcttatca
     5881 aagaggatta gatagcaatc atgtcgaggc tataaaaggt tacgctactt ttatcgatag
     5941 tcaaacgatc gaagtaaacg gtgaaaccta tcgtgctcct aaaatcttga tcgctactgg
     6001 aggccgtgca agtaaaatga ccattcctgg tggagaatac gcaattgatt caaatggatt
     6061 ttttgaatta acagagttac ctaagagagc aatcgtttta ggtgctggat acattgcggc
     6121 tgaactttca ggtgtatttc gtggtttagg tagtgaagtc atgtgggcat atcgaaaaga
     6181 acgaccactt agaacgtttg ataaaatgct ttcggacaat ttgatccaaa tgtaccaaga
     6241 agctggaata aaaacttacg cacatcatat tgctaaagaa attactaaaa acaatgacga
     6301 atatacggtg ctatttgaaa atggtgaaac cttaactggt gattgtgtac tttttgccgg
     6361 tggacgtgta ccgaataccg aaaaattagg tctggaaaat acaaatgtgg aattggacaa
     6421 aaaagggttt atcaaagtag ataaatacca aaatacaacg gatgaacata tttttgctgt
     6481 tggtgatgtg atcggtaaat tagatctgac tcctgttgca atcgcagctg gtagacgttt
     6541 atcagaacgt ttatttaatg gaaagaaaga ttcttatctt gactataaac ttgttccgac
     6601 agtcgtcttt acgcatccac caattgcaac aattggtttg acagaagaag aagcattgga
     6661 aaaatatggc gaaaatgaat tgaaagttta tcgttcacgc tttaccccaa tgtactttgc
     6721 attaaatgat tatcgccaaa aatgcgaaat gaaattgatc tgtgtcggaa aagaagaaag
     6781 aatcgtcggc ttacatgcta ttggtattgg tgtcgatgag atgctgcaag gttttgccgt
     6841 ggctatcaag atgggtgcaa ctaaagaaga ctttgacaat acagtagcca tccatccaac
     6901 aggagcggaa gaattcgtaa cgatgcgata atttaatatt aacagttcca acagcttttt
     6961 attgaacaaa ccatgtaaaa ccagaaaatc agtatactga ttttctggtt ttttgttttt
     7021 tagttattta tgtaagattg cttcgataat tctcaaaacg aaataagtag ggaagttgga
     7081 gattttgatt atcttcgtaa ctgacgaaga gtaaatgaat ccatttggta tgcactctta
     7141 agttcaaaat attggtatcg atgtttaaaa gaaatagaaa tgtatcgaag aacagatgtt
     7201 taaaaaagtg tgactttgta taaataaatt agaagaagca aacgaggatg ttaaaagaat
     7261 accatatagt taccaaacca agaaagttct cgtcaagttc gctggtacga atcaaatcaa
     7321 gtgaaaaaag ggactgtgaa aaaaattgtg tcatagttag tcactttttc cgaacaaata
     7381 gtgttcaatc agctgtcctt cggtattagc tcaaaaaacg gcaaaatatg aggaactatt
     7441 tttccatttg tttctctaat actcaggaca aaacactgat tt
//
LOCUS       DADVXU010000116         7391 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.121, whole genome shotgun sequence.
ACCESSION   DADVXU010000116 DADVXU010000000
VERSION     DADVXU010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.121"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(204..1652)
                     /gene="cls"
                     /locus_tag="KB953_002508"
     CDS             complement(204..1652)
                     /gene="cls"
                     /locus_tag="KB953_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBB6867438.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGEIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            complement(1804..2697)
                     /locus_tag="KB953_002509"
     CDS             complement(1804..2697)
                     /locus_tag="KB953_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6867439.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            complement(2681..3367)
                     /gene="ftsE"
                     /locus_tag="KB953_002510"
     CDS             complement(2681..3367)
                     /gene="ftsE"
                     /locus_tag="KB953_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HBB6867440.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(3472..4573)
                     /gene="prfB"
                     /locus_tag="KB953_002511"
     CDS             complement(join(3472..4500,4502..4573))
                     /gene="prfB"
                     /locus_tag="KB953_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBB6867441.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(4699..7233)
                     /gene="secA"
                     /locus_tag="KB953_002512"
     CDS             complement(4699..7233)
                     /gene="secA"
                     /locus_tag="KB953_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBB6867442.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
BASE COUNT         1976 a         1639 c         1289 g         2487 t
ORIGIN      
        1 gctatactgg ctttgaaatg tgtctctttt aatagcatat attagcttat ttcttgcttt
       61 ccccaaaaaa caagagatac cccaaagtta atttcgctca tgcgagacgc tttctccatt
      121 tctgacgtac tctcagaaat ggccccgtta aaaaaggaca cgagatctcc atctgttgat
      181 ggttctcatg tcctttttat gttttaaagg agcggcgaaa tcaatcttgc taatccttct
      241 ttcatcttga tgattattcc tcggttttca tacatttctt ttgtgagatg cgtagaaatt
      301 ttcgagtctt caaaaaaagc ttttcttaca cgtgaagcca ttcgttcatc ataaaccagt
      361 gtattgacct caaagtccaa gcgaaatgaa cgaacatcga tatttgctga gccaactgaa
      421 acaatgccac catcgataat catcgtcttt gcatgaataa atccattctc atatgtttcg
      481 atccttgctc catactcgat cagctctgca gcgaaagaat acgttgccca gtaaacgaga
      541 agatgatctg gtttgttagg tatttggata tgaactttta cgcctgaaag aagagccaac
      601 ttcagtgctt catgaatcga tccatccggt atgtaataag gcgtttggat caatatctcc
      661 cgtttggcta acgaaatcat tttcaaataa gtcattttga tttgttcatg ttcggtatct
      721 ggtccgcttg tcacgatttg aacagctata tcaccagtag tgtgaatgga cggaaaaaat
      781 ttgggagaat agcctacttc ttttgtatgc tgagaattcc agtccatcaa aaaacggttt
      841 tgcaaggtat acaccgcttc tccgtaaata cgcaaatgat tgtctcgcca ataaccaaat
      901 ttctttacca atcctagata ttcatttcca acattgaacc cacctgtata tccgatttcc
      961 ccatcaataa ctacgatttt acgatgattg cgataattga tccgaggatt aatatacgga
     1021 acaaatagag gaaagaaaaa tgcgacttct ccgcctgctt ttttcaaatt ctgaaaaaag
     1081 ctggaagaaa cttgtgtgga accccatgca tcaagaagga ctctgacttt tacccctcgt
     1141 cttgccgcct ctgtcaatgc atctcgaact tcttcaccta atgcatcact gcgataaata
     1201 taatactgga agtggatatg gtcttctgcc tgcttgatat cttcaatcag cgcatcgaat
     1261 ttttcacgac catctgtaaa taaagtgatt tcatttttag tcgtatagag cgtactttca
     1321 aataccgtga gcatatagat caattgtttc acatctactt gtccagtagg cggatgagga
     1381 taaagccctc gcatcaatgc ctgtttgtct tcttcaattt ccaggttctt accgattttc
     1441 ccttggatct tcaaatcaaa gattttctct ttggaaatcc ctcttcctaa aaaaatatat
     1501 aaaataaatc ctacaactgg aatgaacatc aaaacgagca gccatgccca tgtttgagca
     1561 gtttcctttc tttctcgaaa aacgatgata aaggaaagca aaatattcat aatcaaaagg
     1621 atcgtaaaaa aattgtcaac gataattttc acggcttcac ctcttcttct accaacttaa
     1681 gaatacccaa gttgtttttt aaagtaaagg gaaaaaagcc gcttccttct attctcctag
     1741 ccaataaaaa aagaaagctg gaacgaaact gtccaacttt ctcattttat ttatattgtg
     1801 gtattagaat ttcaagaaac gtctcataga aatgatcgag ccaagtgaac caatgatcac
     1861 accaatgccg accattaaaa gattgatttt ccaaacgata ttttgtggat gaatcaatga
     1921 ataatgggaa cgtaataatg atggattgat caattggaaa aaccatgcat aaccaaatgt
     1981 gatgatcaaa attggaagaa ttgcaccgat cagaccgatc cagccacctt caaggaaaaa
     2041 cggccagcga atatatccat tttttgcacc gactaaacgc atgatttgaa tttcacgctg
     2101 tctagacaaa atcgtgatcc gaatcgtgtt cgaaatgaga aacatcgcta cgaacagcag
     2161 caatgccgct gcaactaatc cccatgtccg aactgcacca gcgatttgga aaatccgatc
     2221 tgaggaagct ccaccgtaat cagaacgaga aacattcttc aaatctgctg cttcttctgc
     2281 tacttgtttt gtataagaag gatcagtagc acttactaca tacacatcat ataacgggtt
     2341 attgtcacct tcaaacaatt tccaggaatc acccatttcg ttttggattt ttttcaattc
     2401 ctgttctttg cttgaaaatt caactttttt aacatgatct aagtcattca attctttttc
     2461 tagctttttc atctcttcgt cttttgtacc gatatctacg aatacagata catcaacgtt
     2521 tccttctata tcctgagcta atttagttgc attcatgata actgccatga atacaccgac
     2581 taaagtcaac gtgatcgtta ccgcactgac tgaagcaact gtcatccagc cgttacgttt
     2641 caaactcttg atactttcta ataaatggcg gaaaaaggtt ctaatcatcg tatccatatt
     2701 ccccttccgc ttgatctcga ataatgcggc cgttttcgat agcaattact cggtgccgaa
     2761 tcgtatttac gatcgtactg ttatgtgtag ccatcacgac agtagttcct tgggcattga
     2821 tccgatctaa taatttcatg atttcccatg agttttctgg atccagattt cctgtcggtt
     2881 catccgcgat aagtacttta ggcgtattca cgattgcccg agcaatagag acacgttgct
     2941 gttccccacc ggataattcg ctaggaaaga ctcgaacttt gtgtttcaaa ccaacaaggt
     3001 ccagcacttc cattactcgt tttttgatat ctcgaggttt tctgccgata acttgcatag
     3061 cgtacgcaac attttcatat actgtttttc ttggtaataa tttatagtct tggaacacca
     3121 cgccgatttc tctgcgtaga taaggaactt cgcggttttt gattgctatt agatcatgac
     3181 ctgcgacgtt caaagttcct tttgaagctt tttcttctcg atacatcaat ttgatgaatg
     3241 tagatttacc ggctccagac ggaccaacca catagacaaa ctctccttgt tcgatgacaa
     3301 tcgagagatt gcggatagcg gtcgtaccat ttgagtactt cttcattaca tccttcattt
     3361 caatcatggc gttctctcca tatctttttc agtctttagc tattatagca tggcaatgtt
     3421 tcaacatgct tgcaaaatct tacaatttta tttcaattta acaatgggtt cttattgtag
     3481 atgcattttt aaatagccgt caataaataa atcaaggtca ccgtccataa ctgcttggac
     3541 atttcctgtc tctacgtttg tccgatggtc ttttaccata gaatagggat ggaaaacata
     3601 agaacggatc tgtgaacccc agccgatttc tttttgttct cctcgaagtg cagctgcttc
     3661 ttgttctttc ttttccactt ctaattgata aagcttagct tttaacatcc ccatcgcttg
     3721 ctcacggttt ttcaattgtg agcgttgtgc ctgactagct actactgttc ctgtaggcaa
     3781 gtgagtgatc cgtacagcag attctgtttt attgatgtgc tgaccacctg caccacttgc
     3841 tcggtacgta tcgattttca aatcatctgg attgatttct atatcgatcg tttcatcaag
     3901 ctctggcatc acatctaccg aacaaaaaga agtatgacgt cgtttcgctg agtcaaaagg
     3961 cgaaatccgt acaaggcgat gaacaccttt ttctgatttt agataaccaa aagcgttatg
     4021 tcctttaatc agcagggtca cactcttgat ccctgcttca tcacctgctt gataatctag
     4081 tgtttctact tgatagccat gctgctctgc ccaacgtgta tacatacgta aaagcatgct
     4141 gccccagtct tgggactccg ttccgccagc ccctggatgc aattcgacga tcgcattgtt
     4201 tcgatcataa ggtccgtcta acagcataga taattcatac gtcttcattt tttcatctaa
     4261 tgcattgata cgtgtttctg cttcttcttg cattgctgca tcaggttctt cttgcagcat
     4321 ctccaccatc aattcgattt cttctagttc ttcggcaaga gaatggaatt ggtcataggt
     4381 ttctttatta gcgttatttt cgctgatcac tttttgtgcg gcttcgctgt catcccaaaa
     4441 accaggttca gccatttttg cttctgcttc cgcgatgtct tcttccagct gatctaagtc
     4501 aaagagacct cctgaaactt gtgacctttt cctgcatttc tgtcaggaga tttcgaattt
     4561 cggatatttc catgtttttt ccttctttca ctatactttt ttcattatat ctagtctaac
     4621 agaatcagca aataacagaa agaaggtgag acgaattctt aaaaaaagaa tttgtctcac
     4681 caatcctgct gtcagaaatt agtttgcatt ttttccatga cagttcttat attttttccc
     4741 gcttccacaa ggacaaggat catttcttcc gaccttattc acatggattg gctgtttttt
     4801 cgcgctagta ttactatttt ctttggcatc tccgtcttct gagtgagaag ctgttccttg
     4861 agaaacctgt tcacgctgta cgttttgacg gatctcagct ttcataaaga ttcgagtgac
     4921 ttcatactcg atggcgccta ccatatcttc aaacatctta tagccttctg tttgatattc
     4981 gactaatgga ttgttctgtc cataagcacg taagccgatt gactgacgta attgatccat
     5041 tgcatcaata tggtctgtcc atttagagtc cacaacacgc aagatcacga ctttttcaaa
     5101 ttccaacagt tgctctggac catttagttg agccgctttg gtattaaata cttcttgtgc
     5161 tcgttcgttt agatactctt tgatctcctg agctgttttt ccttcgatat ctgcaagagt
     5221 aatgctatct tcatggacaa gagtactgcc ggcaaaatcg atgatacttt ccaaattcca
     5281 attttctttc tctaattgtg tatgcgcatc tactacacga ctgattgtcc gtttgaccat
     5341 gttcattagt ggcttggata agcttttttc ttccatgatt acttcttgtc gctgtttata
     5401 gattacttca cgttgttcac gcatcacatc atcgtattgt aagacgtttt tacgtgtgtc
     5461 atagttattt ccttctacac gtttttgtgc agattctact tgtttcgtca gcatcttgct
     5521 ttggataaca gcatcttcct caccgatctt catccgatct aagaaagctt tgattcgttc
     5581 tgaaccgaat cgtttcatca aatcatcttc caatgacaag tagaactgtg acataccagg
     5641 gtctccttga cgtcctgcac gtcctcgaag ctggttatcg atacgacgag attcatgtcg
     5701 ttctgtcccg ataactgcta gaccaccaag ttctcttacc ccaagtccta acttgatatc
     5761 tgttccccga ccagccatgt tggtagcaat cgtcacagca cctttttgac cagcattcat
     5821 gatgatctct gcttctttaa agtgattttt cgcattcagc acttcatgag gaatacgttc
     5881 ttgattcagc atatctgata ataactcaga tgtttcaacc gcaacagttc cgactaacac
     5941 aggttgacct ttgcggtagc gatctttgat atcttgcacg acagcgtgga acttgctttg
     6001 taatgttggg taaagcaaat ctggacgatc atcacggata accggacgat ttgtcggaat
     6061 ctggtatact tggatgttat aaatttctct gaattcttct tcttctgttt tagctgtacc
     6121 agtcattccc gcaagtttct tgtacatacg gaaatagttc tggaaagtga tcgtcgccat
     6181 ggttttcgtt tcgtcttcga tctctacgcc ttctttagct tcgattgctt ggtgaagtcc
     6241 atctgaataa cggcgtccat ccatgatccg accagtgaac tggtcaacaa tcagtacttt
     6301 tccgtcttgg acaacataat cgatatctaa aagcatgata tagttggctc tcaatgcttg
     6361 atccaagtga tgggtcaatg cagtattttc aatgtcgtat aagttatcca aaccgaatgt
     6421 ttcttctgct ttttcgatcc ctgcttcggt taaactgatt gtttttgatt gtacatcgat
     6481 tttataatct tcttcttctt tcaaacgttt cacgaagttg tctgctcttg tatataaagc
     6541 tgtggatttt tctgcttgcc ctgaaatgat cagcggcgtt ctcgcttcat caatcaagat
     6601 tgagtcaact tcatcgacaa tggcataatt cagcggacgt tgtaccattt gatggcggta
     6661 aacgaccata ttgtctctca agtagtcaaa tcctaattca ttattcgtac tataagtgat
     6721 atcacaatta taagcctcgc gtttttcatc cgaagatttt gagttgatat tcaaccccac
     6781 tgttaatcct aagaaattat aaagttcgcc catttccgtc gagtcacggg ttgctaaata
     6841 ttcattgacg gtaactacgt gtaccccttc gccagtcaga gcatttagat atactggcat
     6901 cgttgcggtc aaggttttac cttcaccagt acgcatttca gggatatttc catcgtgaag
     6961 cacgattcca cccatcaatt gtacgtggta tggatataaa cctaatacac gttttgctgc
     7021 ttctcggacg actgcaaaag cttccggaag taattgatct aatgtttctc ctttttggta
     7081 tctgcccttg aattcagcag tttttgcctg taattgttca tcagttaatg ctgccatttg
     7141 gtcagcgtat gcttcaactt tttttgcaat gctatctaaa cgttttagtt ctttcttatc
     7201 gttttcgata agtgaacgaa tcaaattggc catgcgttgt gtaatctcct tttccttctg
     7261 tgtattatcc agactatgta gactttactt ttcattttat cattagttat agataattga
     7321 aatatcttaa gcgtattaaa gtaagaactt cataatttat tcatatttca tctaaaagag
     7381 gaggaaactc a
//
LOCUS       DADVXU010000117         7088 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.122, whole genome shotgun sequence.
ACCESSION   DADVXU010000117 DADVXU010000000
VERSION     DADVXU010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.122"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            142..1989
                     /locus_tag="KB953_002513"
     CDS             142..1989
                     /locus_tag="KB953_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBB6867443.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            1992..2888
                     /locus_tag="KB953_002514"
     CDS             1992..2888
                     /locus_tag="KB953_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBB6867444.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            2938..3327
                     /locus_tag="KB953_002515"
     CDS             2938..3327
                     /locus_tag="KB953_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBB6867445.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3314..5659
                     /locus_tag="KB953_002516"
     CDS             3314..5659
                     /locus_tag="KB953_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBB6867446.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLENGITSI"
     gene            5752..6939
                     /locus_tag="KB953_002517"
     CDS             5752..6939
                     /locus_tag="KB953_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HBB6867447.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT         2405 a         1093 c         1490 g         2100 t
ORIGIN      
        1 gggaattatt tggttacgag tcgaggggtt tttgaactaa tgtaaatcat ttgttagaat
       61 tgaccagaaa tttctatcgt acttttactt ttctatttga tatactgaaa tagaaaatat
      121 ttgttgatag gggtgttact aatggttggc aataagtggt acaaatgtga tcttcatatt
      181 cattcaactg cgagtcagtg ctttcaggat aaaaaggtta ctgctaagga atgggtagaa
      241 gaatgtttaa acaaaggact cgattgtgtt gcattaactg atcacaatac aggggaaaat
      301 attgatgaat ataagaaagt ggctatcgaa aatggcttag atttttttcc tggagtggaa
      361 ttaacgtgtg gagaaaatgg aactcattta ctagtcctat ttgataaaag tgcatgtaca
      421 aatgatatct ctgatttctt aaccaagatg ggtataaaaa gagaggattg ggccagcgct
      481 gcagcttgct caatgaaatc agttttagaa gttattgatt ttgctgagca cgagggcttg
      541 ctcgttattc ctgcacatat agatgagttt aatggaattt cttcagcaaa atttggaaat
      601 ctaggaaaga tatttgaatc agaaaatata tatgcagtac agacggttca aaaagaattt
      661 tttgaaaatc gctcacaaat gattccctca tctaagcgtg acactatcta tgatagtgtg
      721 aatgccagat atgatggtag agttggaaaa gatactttgg aatcttggta taaatcagtt
      781 gttgaggcag aaaaaaataa tatgacattt cttagctttt ctgataaccc acactcgaaa
      841 ggaaattcaa aacatggact ttggggaata ggcacaagat attcctatat aaaaatgaag
      901 gacgaacctg acttgggatc acttcgcgat gctttaatgc taggcgaaac tcgagttcat
      961 tctgattttt ctaatttctc cattaatgaa aatgaagttt tattggaaaa attaagcttt
     1021 tctgggacca ctcttagcac aaaagaagtt gtagtcgaat tcagcgataa tttaacttca
     1081 attattggag gaagaggaac aggcaaatct tgtattacaa gattcttggt ctatgtcctt
     1141 ggaaaggaag cagaacttga tcaattttct gaaatacaaa gtgattatca gaactttgca
     1201 caaattgaac ataatggctc tggaatattt ttgaaggata caattgtgaa actgaatatt
     1261 ttctataagg ggacaaaata tcaaataata agaacacagg atagacattc tatttatgaa
     1321 tttacacaag agaatggtct ggttgaaaaa gaaacagaac ggttgagaat gatttccgat
     1381 aaggtgagta tatactctca aaaacaaatt tatgaaattt caaaaaatca aactagtata
     1441 ctagaattac tagatggata taactctgat ttgatttcag agtataaaaa tgagattgaa
     1501 acatgtgtaa acgaaataag aaaattaaat tgggatattg tttctgtgaa aaaagaaata
     1561 caagataagg cgaaggtaga gcttgaaatt gaagatttaa aaaaacaagt agaaaagcta
     1621 tctcataaga gttataaaga tgtttatgat gagtattcaa aagagacaga tatttatagg
     1681 gaactgaaac gtgatgtgga ggcacttaag gaaattccca aaaatctaac tgatagtgtt
     1741 gacaaaattg actttggcaa tggtataaaa gttacagacg aaattgatga acaccgcgga
     1801 gaattgatta agaatttagt gaccaatcgg gctaagattc aaacaataat taatgaaatg
     1861 tcagatgaaa ttgatagtta tagagcagag ctgaataaat cagattggaa agttaactat
     1921 aatgaggttt caaagaggta tcaaagaggt atcaaaaact tagtgaagag ttatctcaag
     1981 atgaattaaa aatgttacaa aaccttgatt ctttatatca atctttatca aaaactgaag
     2041 agcgattgga agaaattatt caacaagaaa taaagcttaa agattaccaa gttgaggttg
     2101 actcagcaat gtcaagaatg aataaatcat atcaaaatct ctattctgag agaagtagtt
     2161 cctcgcgcga aattttttct gatattacag gaatagaggc tactattcaa ccaaataggg
     2221 atttcaataa ttatgttaaa gagctcagag cttgtattaa taaggaggac acttttgaca
     2281 gtgagtttgg atctcttatt gataagctga caaaaaattc cattactaat caagatatat
     2341 ataatgacat acttgatata agagttagtg gggaaagtag tattttttca gataaaaaat
     2401 taatcaattc tataagaaaa ttgaatgatg cacaagttct tgaattacaa acccttaagc
     2461 caaatgattt agtagttatc cgacttgaac taaatggaaa aaaagttagt ttatcgaatg
     2521 ctagtgctgg tcaaaagaca tctgcaattc tttctatgat tttggcgtat ggaaattcac
     2581 cactcatatt ggatcagcct gaagatgatt tggatagtca attaattaat aacttaatcg
     2641 taagaagtat cattacaaaa aaggaaaaca gacaaatttt aattgtaact cataatgcaa
     2701 atatcccagt taatggtgat tcagaatggt tgattagcat gggagattca aaagaaatat
     2761 cggctgatca gtgcgggtcg gtagatgaac ccaatatcaa gccaagaata tgtactgtga
     2821 tggaaggtgg cgaggatgct tttacaaata gagcgaaacg ctatggtttt aaacaaattg
     2881 tagaataaca ctagagggtg agacatatta ttatgtccgc cctttttagg aggaaaaatg
     2941 aaaaaaataa aaagctatac cagtatctgg gcagttgaaa aagtcattta cgcaatcaat
     3001 gattttcagt tgccttttcc tgtgactttt aaccaaatgg cttggttcgt tttatcttta
     3061 ctgtttgtca tcgtatttgc ccatgtgcct ccactatcta tgatagagga ggtcttttta
     3121 aaatacttag gcataccggt ggctgtcact tggtttatgt cgcaaaagac gtttgatggc
     3181 aagaaaccac tgggattttt acgatccttt atcagctatc acttgcgatt taaggtgact
     3241 tttgcaggaa agaaagtcaa agaacagaag aaacgatggg atgaacccag tactcttgta
     3301 aggagtgtga actatgtatc cgattaagta tattgaaaat aatctcgtct ttaaccaaga
     3361 aggcgagtgt tttgcctact atgaattagt gccttacaat tactcctttt tgtcacccga
     3421 acagaaattt caagtgcatg ataactttcg ccaactaatt gcgcaaaatc gtgaaggcaa
     3481 gatccatgcg ttacaaattg ccacagaaag tagtattcgc gccacacaag aacgatcaaa
     3541 aaaagaaatt accggtagac tcaaagaagt agcgaaacaa cgaattgata tacagacaga
     3601 tgcattagta tccatgattg gcgatagtca gattgattat cgctttttta ttggctttaa
     3661 actaattgcg acagatgaag aagtcaatct aaagagtctc aagaaatctt tcttttctgg
     3721 gcttcaagaa tttgtctatg gagtgaatca tcatttgatg ggcgattttg tttccttatc
     3781 caatgaagaa attcgctgct attctaagct tgaaaaatta atggaaagca aattggctcg
     3841 aagatttaaa gtcaggcgag tcacaccgag tgacctgacg tatttgattg aacatattta
     3901 tggcgaaaaa gggataccgt ttgaagaata cgaatttcag ctaccaaaga aaaagctaaa
     3961 atcagaaaca ctggtgaaac gttatgactt attacgtcca aatcgttgtt taattgaaga
     4021 aaaaccaagg tgcttacgga tggaacatga gaatcatgag tcgtacgtag cgtatttaac
     4081 aattaataca attgtggggg agatggaatt tccttcctca gaacttttct attaccagca
     4141 acaacaattt acattcccaa tcgacacaag tatgaatgta gagattgtga cgaataaaaa
     4201 ggctttagct accgttcgta ataagaaaaa agaactgaaa gatttagaca atcatgctta
     4261 ccaatctgac aatgaaacga attccaatgt cttagatgct ttggattccg tggatgaatt
     4321 ggaaaccacc ttggatcagt caaaagaatc tatgtacaaa ctgagttatg tggttcgagt
     4381 gagtgccgaa tcagtggatg aactaaagcg acgctgtgat gaagtgctcg atttttatga
     4441 tgataccaat gtaaaactag tccggccctt tggcgatatg atggggctac atgaagaatt
     4501 cttaccctca agtaaacgat atatgaacga ctatattcag tatgttacgt ctgatttttt
     4561 ggcggggctt ggttttgggg ctactcagat gctaggagaa ttagaaggga tttactttgg
     4621 ctacaatgtg gatacaggtc gtaatgttta cctgaaaccg gcattggctt cacaaggagt
     4681 caagggttca gtaaccaatg cgctggccgc cgctttcctg ggttctcttg gtggaggtaa
     4741 atcctttagt aacaatctct tagtctacta tgctgttttg ttcggtggtc aagctgtgat
     4801 tgtcgatcca aaaggggagc gaggagggtg gaaagaaacc ttgccagaaa ttgcggatga
     4861 aatcagtatc ctcaacttga cgagtaagtc agaaaatcaa ggtctgcttg atccttatgt
     4921 gattatgaag aaaaagaaag actcggaaag tttagcaatt gatattctga cttttttaac
     4981 aggaatttca agtcgagacg gggagaagtt tcctgtgtta cgtaaagcca ttcgaacggt
     5041 gactcaaagt gatcaacggg ggttattaca agttattaac gaattgcgaa aagacgggtc
     5101 accaatcgcg gaaaatatcg ccgatcatat cgagtccttt accgattatg attttgctca
     5161 tttgcttttc tcagacggct cgattaccca atcgattagt ttagataaac agctaaatat
     5221 cattcaagtg gcggatttgg ttttaccgga tgcagaaacg acttttgaag aatataccac
     5281 catggaattg cttagtgttt cgatgctaat cgtcatttcc acttttgcct tggactttat
     5341 ccatagtaac cgagaaatct tcaaaattgt cgatttggat gaggcttgga gttttcttca
     5401 agtggctcaa ggaaaaacgt tatcgaataa gcttgttcgt gctggtcggg ccatgaatgc
     5461 tggggtctat ttcgtcactc aaaatagcga cgatttatta gatgaacgtt taaaaaataa
     5521 tatcgggttg aagtttgctt ttcgcagtac cgatattcat gaaatcaaga aaaccttgga
     5581 attctttggt ttggatcagg aggatcaaag caatcaaaaa cgcctgcgag atttggaaaa
     5641 cgggattacg tccatataat tgtgtaaaag tgaagcatca aaaaagccat tgatctcagt
     5701 taggagtaca atgttttttg cagaaaacat actcgaatag gactgatatc aatgactcaa
     5761 gtacatttta cttttgaaag cgaagaaatt caagcaatta taaacgaaag cggtgcgaat
     5821 gataccgcaa aaacacttat gactatcatg tttaatcagc tgatggaaga acaacgaaat
     5881 caatatattc aggcaaatgc ctatgaacgt tcagaagaac ggcaatcaca aagaaatggt
     5941 tactacgatc gttcgtttac aactcgaatt ggtacgttag aacttcatgt tccacgaacc
     6001 agagacggta agtttagtcc tactatcttt gaaagatatc agcgaagcga aaaagctttg
     6061 atcgctgcca tgattgaaat ggtcatttct ggtgtctcca ctcgcaaagt aactaaaaca
     6121 gttgaactgc taacggatgg tgcgactgtt tctaaatcat tcgtttcaaa tctgatgaaa
     6181 cagttggatc catttgtttt tgagtggaga aaccggagcc tagaaggctc ggaatatcca
     6241 tttttcatgt gtgatgccct gtatatgaaa gtaagggaaa atcatcgtat tgtctcaaaa
     6301 ggtgtatata ttggtatcgg cattgattct gatggacgac gtacgattct tggttttgat
     6361 gttcaagacg gcgaatcgga agataattgg gataccgttt ttcaatcgtt cgttcaacga
     6421 ggtttgttcg gtgtaaaact ggtcatttct gatgcacaca aagggttagt taaggctgtt
     6481 cgcaagaact ttttaggtgc gagctggcaa agatgccaag cccacttttt gagaaatatt
     6541 tttgataagc tgccaaagaa agtttcttct gatgtaaaag acgagttgaa gagtatcttt
     6601 aaagcctctg aacttgaatt gacacgtgaa agaaaagagc acttcttgga aaaatatggt
     6661 tgtgattcaa aattaagtgc tgcttgcgac attttagaaa acggctttga agatgctatt
     6721 caaattttgt cttttcctga aaatatccgc cgaaggattc gtacaaccaa tgtgttggaa
     6781 cggttgaacg aagagattcg ccggagagaa cgagtgattc ggatttttcc aaatatcaat
     6841 tccatcactc ggataattgg aacacttttg atggaaaaag acactgagtg gctggcttct
     6901 ccacgaaaat atttagaatt taattcgaat aacatttagc accatgaacc tgtgcgagtc
     6961 tcttctttct gaggagagac atcaagagtc gctgcgctac tgctcctgac ctctctcctc
     7021 agaaagaaag gcatggcagt taggttcaca acgcaaaaca ttgtgctttt gatgaggttg
     7081 aactttta
//
LOCUS       DADVXU010000118         6836 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.123, whole genome shotgun sequence.
ACCESSION   DADVXU010000118 DADVXU010000000
VERSION     DADVXU010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.123"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            69..959
                     /locus_tag="KB953_002518"
     CDS             69..959
                     /locus_tag="KB953_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HBB6867448.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(1032..1856)
                     /gene="nadE"
                     /locus_tag="KB953_002519"
     CDS             complement(1032..1856)
                     /gene="nadE"
                     /locus_tag="KB953_002519"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HBB6867449.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1868..3340)
                     /locus_tag="KB953_002520"
     CDS             complement(1868..3340)
                     /locus_tag="KB953_002520"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBB6867450.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(3977..4339)
                     /locus_tag="KB953_002521"
     CDS             complement(3977..4339)
                     /locus_tag="KB953_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HBB6867451.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            4562..5314
                     /locus_tag="KB953_002522"
     CDS             4562..5314
                     /locus_tag="KB953_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBB6867452.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(5454..6377)
                     /locus_tag="KB953_002523"
     CDS             complement(5454..6377)
                     /locus_tag="KB953_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBB6867453.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(6392..6664)
                     /locus_tag="KB953_002524"
     CDS             complement(6392..6664)
                     /locus_tag="KB953_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867454.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         2096 a         1354 c         1163 g         2223 t
ORIGIN      
        1 taatttaata taacttttat taacaaatat gttaatatat aagaaaaacc ttttaagagg
       61 tgatatatat gcaactacaa ataaacgaag aatccttacc ggtatatgaa gcattagcta
      121 gtaaaacaag gatcaagatc atccagctac tttccaaaaa aaagatgaat gtcaaagatt
      181 tggcaaaaga acttggagtg agcagtgcta tcacgacgat gcacgtgaaa aagctagagg
      241 aagcaaacat catcaaaaca gaaaaagtag gacaacaaaa aatttcgagc cttcgtgtcg
      301 ataaaatcga tatcagtttt ccagaaaaaa tattcaatgc ttttgatacg aaagaaacat
      361 cgatacctat tggtcactat acaaattatg caattgaacc aacatgcggt ttagctacga
      421 tccatgattt tatcgggaaa gtggatgaac cacgctattt tatggatcct cgaaggatgg
      481 atgcccgcat tctctggttt accagcggat ttgtcgagta tcaggcacct aattttttaa
      541 atacagagga tacattggag atgttggaag tttctgtgga aatttcttct gaatttcctt
      601 tttcaaatga caattggcct tcagatatca ctttcagcct aaatggtgtg gagctaggaa
      661 catggacgag ccctggagat tttgctgata tccgcggtaa atatacgcct gattggtatc
      721 cagataatct taatcaatac ggcttgctga agacgatccg aattaccaaa cacttgacga
      781 atatgaatgg agaacccttg tcaaacatca cgatcaacga catccctaaa gagcaagaca
      841 cttggcattt gcgaatcgaa gtgaaagacg atgccaaaca tgtggggggc tgtactttat
      901 ttggtaaagg attcggtaat tacgatcagg atatcaaatt aaaagtttac tacagctgat
      961 atttttcttt tctattttat aaaacaaaaa cagccaatcc ctatgaaggg acggctgttt
     1021 ttgctttatt gttatttcca gaaatcatcc aaaacggaaa ttggcaaatg tcgtttatgt
     1081 tgtgtcttgt tccaccatgc ttcgattttt tcttgtgctt gagcgggaac ttcttttcct
     1141 tctagataat catcaatttc attatatgtg acacctagtg cgacttcatc tgcaatcatt
     1201 ggtttcccat cttcaagatc tgcagtcgga atttttgtgt ataatttttc cggtgcattc
     1261 aatgcttgaa gcaattgttt cccttgtcgt ttgtccaaac ggaataatgg caaaatgtcg
     1321 gctccaccat caccaaattt cgtgaagaat ccagtaatgt tttctgcagc atgatctgtc
     1381 cctaatacgg ctcctgctct ttctccagct acggcatatt gcgtaatcat acgttgacgt
     1441 gctttgatat ttcctttgtt aaaatcgctg attcccacac ctgcttcgct taatacttgt
     1501 acttgtgcat caacagctgg tttgatattt actcgcagac taacatcggg ctggataaag
     1561 tcaagagcag cttttgcatc ttcttcatct gcttgttcac cataaggaag acgaacagcg
     1621 ataaattgat agttttcatc ttttgtttct tcacgcattt cttccatcgt tagttgcgcc
     1681 aaacggcctg ctaaagtaga atcttggccg ccgctgatac ctagtacaaa agttttaaga
     1741 aacggatatt ttttcaaata tgctttcata aagtcaatac ttttacgtat ttcttctttt
     1801 ggatcaattg ttggtttgac acctaattca ttgataattt ctgtctgcag gctcatttat
     1861 tctcctccta tgcttctgta tgaagttctg ctacgctttt tttcattcgt tccatgatag
     1921 ccattttatg gttccagcat tcagtagaca gatcgactgg gtatttttgc ggattcaagt
     1981 ctcgtttata ctcttcccat agtgaatcta gattttcttt tgtatattct ttgatttcgt
     2041 ttagtgcagg taattcgtaa actctttttc cttcgataaa gatatcttgt aaaactgggc
     2101 gagctgtaaa gtctcgaaca gttttattga taaacgtatg cactggatgg aacatgaaga
     2161 tagcttcttc ttctcgaggg tcttcatccc ataatgtcac gtagtctcct tcagatttcc
     2221 catcaaaatt tcgagtgatg cgccacactt gttttttccc tggagtagtt actttttctg
     2281 cattgcttga taacttgatc gtatccatca tttttccttc atcatcttcg atcgatacta
     2341 gcttgaatac tgcaccaagg gctggctgat cataggctgt gatcaatttt gtcccaacac
     2401 cccatacatc aattttagct ttttgcatct ttaaattcaa aatcgttgct tcatccaagt
     2461 cattcgaagc atagattttt gcttccgtaa atccggctgc atctaattgt tcccgaacac
     2521 gcttagatat ataggccata tctccactgt cgatgcgtac gccttggaag ttgattttgt
     2581 cacctaattc tttcgccacg cgaatcgcac ttggcacacc tgatttcaac gtatcataag
     2641 tgtcgactaa gaacacgcag tctttatggg ttttagcgta ggccataaat gcttcataat
     2701 catttccata agattggact aacgaatgag catgggtccc gctagctggt atcccaaata
     2761 tcttcccagc acgtacattg cttgttgcat ctgctccgcc gatatatgcc gctctagttc
     2821 cccagactgc tgcatccagt tcttgtgccc gtctagttcc aaattccaat agtggatctt
     2881 ctccgatgac agatttgata cgagctgctt ttgtcgcgat caacgtttgg aaattgacca
     2941 tattcaacag tgctgtttct actaattggc agtgcgctaa aggcccttcc acttggatta
     3001 atggttcatt tgcaaaaact agttctcctt cacgagcaga acgaactgtt gctttaaatt
     3061 caaaattttg taaatactcc aaaaaacctt caggatagac ttctaatcca cgcaagtaag
     3121 caatatcgga atcattgaac gtcagattct ctaaataatt gactaaccgt tccaatcctg
     3181 caaaaaccgc gtatccgtgg ttaaaaggca tttctcgaaa gtaacattca aaaacagcat
     3241 gtaaatcagc tcgacctaat tcccagtatg tctgcatcat attgatttga tatagatccg
     3301 tatgcagcgt caaactgtcg tctgggtaag tattattcat cttgtattac tcctcttggc
     3361 aagttttcat ttcctaccca ttataacaaa aaaaattaat aggaaaagaa atcttccatt
     3421 ttttctgaaa aagcacatgt tttacggata tttcttgcta aaaatgaact ccatctgcta
     3481 aaactatcct ttcttattgg tatttaacct aaacgagaaa gaatacttat tttattcaat
     3541 gtatccgaga cacgaagaaa ttagcgtttt ctatttttct tacttgataa aataagtaga
     3601 ttttcctact acaatcgtag ccgttaagat accgatgatt gaacaaatat aaccaaatgt
     3661 ctctacaaca taatcaatgg caaaagtttt tgaagcaata atacgtcttt ccttttttgc
     3721 ttcttatccc caaagaaacg gatataactt attatgtatt gaattttttt ggaaaggcga
     3781 attatgacaa gatatacctt gcgttgaaaa aactcgcgga aaaaatatca cacgttttta
     3841 aaaagataca aagtaagaaa gcagctgtca atagaatgga aacaaaaatg gcgagacatc
     3901 aatcagtctc gccttgtttt ttattgtata cattagatat ggagcaggtt accataaaga
     3961 tgattattag tcgttattaa atagaatcct tcgtattttt ttgttctatc actacaatat
     4021 ctttatttgg acgtttgaat tgtattccgg cacgttctag ctcctgtttc actcctacat
     4081 ggactttgac atcttctcgt gtcattttta aaacgtgatg aatcgcatag tcgatttctt
     4141 gccttgtgat ttttttcttc gtattgatca tcacgacttg ctttttatca tatgtgtcta
     4201 ccaatagcag ttgtgttcga tctaacgaaa ataatttgta ataattaata gccgtattcc
     4261 caaaaaagaa ttccactact ttaggataaa atgtcttaat atttaaatta ctattcacag
     4321 gtgtttcgat tagcttcatc tcattcctct tttctatata gtcgatttag atccaatctt
     4381 aagttatgtt accatctttc ctagcattaa ggaatcggtt acaattgaac ctttaaagac
     4441 aaaaggattt accttttatc acttaatagt ataacataat ttcgtgagaa aaaaatacaa
     4501 ttttttcttg accatctcgt tacgtaaagg attatgctta catttgtcag gaggaaaaat
     4561 aatggagtac acaataaaga aaatgtcaga gatatcagga gtcagtccga gaaccttgcg
     4621 cttttatgat gagattggac tgctaaaacc agctaggatc aattcttcag gttatcggat
     4681 ttatggcaaa aaagaagtcg atcgtctgca acacatctta ttttatcgaa caatggcatt
     4741 caagttggat gatatccaag aggtcttaga caatccatct tttgaccacc agaaagcttt
     4801 aatcaagcat cagcaaatgt tgctggaaaa acgggcacaa atcgataccc tcttgacaac
     4861 tgtccaacag acacttgata tgtacgaagg aggaagaaaa atgagtgaca aagaaaaatt
     4921 tgaagggttt aaacaacaaa agttgaaaga aaatgaagaa tcctatggac aagagattcg
     4981 acaaaaatac ggaaaaaaaa aggttacagc atccaatgaa aaatggatga atatggataa
     5041 agaaacgtat gaagaaatgc aaactgttga aaagcagatg ttatttgatc tcactcttta
     5101 cttaaaagaa ccagaagatg aaagcttagc agaccgtatc ttccaagctc ataagaagtg
     5161 gttctcttac agctggcctg aatatcagcc aaaagcgcat aaaggactag gaatgatgta
     5221 cgaagccgat gaacggttca ctgcatatta tgacgagcga agtggtcatg gtgcagcaaa
     5281 agctttaaat gaaattatcc aaaaaaaggc gtaatctttt acatgatcca acaaaaaagt
     5341 acacgtaaaa tgacctaata acaaaacagc agacacccaa gttagatttt tatgctaact
     5401 tttggtgtct gctccattct ttcacaagtt ttttcatcct actttaaata tattcatcgt
     5461 tgaataaacg tataaggtat tttcacttta tccttttttt ctgtaagaaa caattgaaat
     5521 gccatttctc cgcaagcttc cagatggtgg tcgatggttg ggaaaccaag aagctcgctg
     5581 atcagcaaat tatcgcgtcc aatcaccact ggtgtcttat cttgcagata gctttgcaag
     5641 atgcctgctg caaccatatc cgaatttgta aagacaccat caactccaag ctctttaaaa
     5701 aagaagcctg cttgttttcc tttctctgca tctatacaat cccaaaaaac aagtgattca
     5761 tcaaaagatg gaaaaagttg atgacatagc tgcaaagtga tttttgagtt ataacttagc
     5821 ctgccacttc gccccaacgt gatgcccaat tgctttatac cttgctgctt caaataagta
     5881 agaccttctg taattgattg ttctcgatca ataaagacac agcttgcctg ttttcccttg
     5941 atttcttcac aaaaaactag aggtccatat ttttgatatg ccaataaatc atctagtgta
     6001 ttggcacgag acgtaatgat caatccatcg aaagctttag ctgcaaattc ttcaagatac
     6061 cttcgctctt tttctttatg atagcccgtc ggtaaaagtg tcactttgta atcataatca
     6121 aacgctgctt caatgatccc actgatcaac tgatcaaaat acggatgatt ggtataaggc
     6181 agaatcactc caatgctttt cgtttttcca taacttaact gtctggcatt tgcattaggg
     6241 atataatcga cttctttcat gaccgcaagt atttctttcc ttttttcctc atctacatat
     6301 ggatgtccat tgattacacg tgaaatagta gaaactgaat aacctgtgag ctttgcgata
     6361 tctcgaatgt ttgccactgc tgcttcgcca cctatacaaa aagattttct tgtaaatagt
     6421 ctaccacagc tggaccgcat tcaaaaaact gatacatttt tccttcatgc gaaaaactga
     6481 tccgctgccc gccccaatat atatcttttg tcaaattttc gatttcattc attggtattt
     6541 cttttactat attcctattc aacccttgaa aactgtacaa cgttaatacg gatcccttga
     6601 actcgacaaa cgcataaaaa taattgttcg ttgtgccatc tgttatctct acataagtat
     6661 tcattgcaag ccctcctttt gatactttga gtatagtgta tgaaacgcgt ctcatagcaa
     6721 ggagtttgtt ttgccaaaga aaagttttat tcctctttca ttattaccaa agtctcttat
     6781 tttaaattga atacattata atgatactgt aaatatatct ttattattag tgaatg
//
LOCUS       DADVXU010000119         6789 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.124, whole genome shotgun sequence.
ACCESSION   DADVXU010000119 DADVXU010000000
VERSION     DADVXU010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.124"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(57..1793)
                     /locus_tag="KB953_002525"
     CDS             complement(57..1793)
                     /locus_tag="KB953_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBB6867455.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(1790..2494)
                     /locus_tag="KB953_002526"
     CDS             complement(1790..2494)
                     /locus_tag="KB953_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBB6867456.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            2625..3128
                     /locus_tag="KB953_002527"
     CDS             2625..3128
                     /locus_tag="KB953_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBB6867457.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            3088..3426
                     /locus_tag="KB953_002528"
     CDS             3088..3426
                     /locus_tag="KB953_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HBB6867458.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            3447..4865
                     /gene="ffh"
                     /locus_tag="KB953_002529"
     CDS             3447..4865
                     /gene="ffh"
                     /locus_tag="KB953_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBB6867459.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            4977..5555
                     /locus_tag="KB953_002530"
     CDS             4977..5555
                     /locus_tag="KB953_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HBB6867460.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            5559..6080
                     /locus_tag="KB953_002531"
     CDS             5559..6080
                     /locus_tag="KB953_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBB6867461.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(6159..6713)
                     /locus_tag="KB953_002532"
     CDS             complement(6159..6713)
                     /locus_tag="KB953_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBB6867462.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
BASE COUNT         2182 a         1278 c         1417 g         1912 t
ORIGIN      
        1 tctttcgaag caggatgagt catccaactt taaaacgcac gagcagttgc tcttttttac
       61 attttcggaa tggtaacggt gaaagtcgaa cctgttccaa gatgactgtc tacagtaatc
      121 gtaccattaa ggagttgcac gtagtctttt acaatcgcta accctaatcc ggtcccgcct
      181 gaatgtcggc ttcgcgcctt gtctacgcgg taaaaacgct caaagatccg ttcctgatcc
      241 tcaatgtcga tacctatacc aaagtcttgg acagaaaacg atagatcatc tgttgcctga
      301 tagcggataa taatctcgct gtcgcttttt gaatattgga cagcattttc aattagattt
      361 ttgataattg gataaaggat atctgtttta gtagtaaatg gttcgttctt tccaatcaat
      421 cgaatcgtca accgtttctt ttcgatgatt ggctgataat tttgaacgat ttgctggaaa
      481 taaggttctg tataaattgt ctggatctcg taagaattct cgccatcttt tgacagctgg
      541 atgatttccc gaatcagttt atctaaccgg atcgcatctt tttgcatgat ctcaagaaaa
      601 gaagtcagag tttgcgggtc atcttttgca ccatccagta aagtctcggt aaaaccaatc
      661 agtgaagtca caggagtttt taattcgtga gagacgttgc caacgaaatc cttttgtagc
      721 ttttctaaac gtcgcacttt cgttaagtca tacgccaccc ctagaatctg tccggtacct
      781 tctgaattag taaagtagcg caaactcaag tctaatgtct gccgaccttc tgtaagggtg
      841 atctcctgat ggatcagcgg atgctcagga gtcacttgat gaatcagctg aatgagtttt
      901 ggctcttgaa tgacttctgt gtaccgctgt tcaggttgat agcttaccac gtttaaatgt
      961 agctgcatct ttggattaag cagtagtaaa cgagaatcag catcatcaat caggaatacg
     1021 ccaatcatca gttcgttcag taacgtatat aattgctctt ctgtggtagt acacgcccga
     1081 tacatcttac tcatttgttc gctcaacgca ttaatcgtct gatacaattc ttcccattga
     1141 tcgggtgttt gcatgattac ttccgcctga ttaggatttt gcaccatttt ttttagtacg
     1201 ggcagcacgg ttttcaatgg ctggtttttt tgatagatca gataatagat cattcctgca
     1261 agaacaagga agaaagacag aaagaagaaa aagacccaac ggcgaaaact ctcagttctc
     1321 ggtaaaaaac cgcttgtcgg ttctgcgata cgtaaaatgg cttctaactg gccacttttt
     1381 ttgactggta atgcgacata taaaagatcc tcattcaaag tagtactttt gcgcaaagcg
     1441 ctacctaggt tcccaccatt caatacggct ttgatttcgg gacgggagtt tcgctgttca
     1501 ttcaatgttt cattgtttgt gtcaaataag atctttccag tagcatccat taaagtaatc
     1561 cgttcgttcg actgatggac gaattcttcc aaagcagttt tgttttgcgc tgcttggagg
     1621 ttttcgacat ctaattgccg gatcagcagt tgtgcttttt ttgtcaggta gctttcttgc
     1681 tgttccacga tctgctgttg aaaataatgg gaaatcaatt gccagccacc aaaaaacaaa
     1741 ctaactaaaa gaataagaaa gaagagacgt tcataaattt ttttccattt catcgtttgg
     1801 gttcctgaaa cttatagcca aatcctctaa tagtcaccaa gtactttggg tgttttggat
     1861 ctcgctcgat tttttctcgc aaatgactaa cgtgtacatc tacgattcgg ctttgtcctg
     1921 cgaagtcaaa attccaaatg cggtctagca atgtgtctcg gtcgatcaca cggtcttttc
     1981 gtttcatgaa ataaacaagt aattcaaatt ctttcggtgt tagttcaatc agatggtcgt
     2041 ctacggtcac ttgataattg gtcaaatcag cagtgatttg tccaatcgat agatattcag
     2101 gctcagcttc atcttttgat tgcttacgag gttcaattcg tctgaatatt gctttcatac
     2161 gggctaatac ttcgcgagga ctaaatggtt tggtcaaata gtcgtcagca cctatttcta
     2221 ggccaatgat ccgatccact tggtcatctt tagccgtcag catcaaaatc ggtgtttcat
     2281 ttttttcttg acgcagccgc tgagtgatct ccatgccatc catcgttgga agcatcacat
     2341 ctaaaatgat gaaatcaaat gattgttcta atgctaattc taatccttcg cctccatctg
     2401 tggcactggt taccttgtag ccttcttttt ccaaattgaa cgtcaagagc gttacaatag
     2461 aaggttcatc atcaacaact agtatttttt tcattcaaat ctcctttaaa taggtttcat
     2521 tctgtttata ttttagcata gaatcagtac aaagggctat tttacaaaag aaataaatgg
     2581 tataatagga aggcactttg ctgaatagaa aggaagagca gtcaatgatt ggaatcagtg
     2641 catgtttagg cggtctggcc tgtcgctatg atggacagaa aaaagaagta ccagaattga
     2701 tggaactagt tgaaactgga gaagctgtca tggtctgtcc agaagtgatc ggcggattac
     2761 caattcctag agatcccgct gaaatcattg gtggaaatgg attcgatgtg tggaaagatc
     2821 aagcaaaagt atggacgaca agcggcgaag atgtaacgga agcgtacaaa aatggcgcga
     2881 tcaaagccta tcaaaaattg caggaaaaac acattgatct agtcatcatg aaggaaaaaa
     2941 gcccttcatg cggaacccat tcgatttatg acggaagttt ttccggaata aaaaaaaatg
     3001 gtgccggagt ggctactgct tattttattc agcaaaaaat gactgtgctc tctgatgaag
     3061 aatggctgaa aactagaggt gagcagaatg gaaatcgaaa agaccaatcg aatgaacgcg
     3121 ctctttgaat tttattcgac cttattgaca gaaaaacaaa tgaactacat ggaattatat
     3181 tatgcagatg atttttcatt aggtgagatc gcggaagaat acgaagtaag ccgacaagca
     3241 gtatatgaca atatcaagcg gacaagcaag atcttggagg attatgaaaa aaaactccat
     3301 cttttttcta attatattgt tcgagaacag gtattggaaa atatgactgc atatattaca
     3361 gataagtacc cggaagataa aaaactactt gaatatgtac agcaaattca agcaatcgaa
     3421 gaataaataa aaggaatgaa gaaaatatgg catttgaaag cttaactgaa cgtctccagc
     3481 aggcgatggg aaaaataaga aaaaaaggaa aagtatcaga agctgacgta aaagaaatga
     3541 tgcgggaaat ccgccttgcc ttactagaag ccgatgttaa cttgcaagta gtcaaagatt
     3601 ttactaagcg cgtacgcgaa cgtgcggtag gggcagaagt gctggaaagt ttatcacctg
     3661 cacaacaaat cgtaaaaatc gttgatgaag aattgacgat cacacttggt tctgaaacag
     3721 cagagttaaa taaatcacca aaaataccaa ctgttatcat gatggctggt cttcaagggg
     3781 caggtaaaac gacctttact ggtaaattag ccaattattt aaagaaaaat gaaaatgctc
     3841 gtccgctcct aatcgcaggg gacgtatatc gaccagctgc gatcgaccaa ttgaaagtat
     3901 taggtcaaca gctagacgtt cctgtatttg atatgggaac tgaagtcagc ccggtagaga
     3961 tcgtccgtca agggatggag ttagcaaaag aaaagaaaaa cgactatgta ttgatcgata
     4021 cggcaggacg tctgcatatc gatgaaacat tgatggatga gttgaaacaa atcaaagaat
     4081 tgacgcaacc aaatgagatt ttgcttgttg tcgatgccat gactggtcaa gatgccgtca
     4141 acgtagctga tagcttcaac cagcaattag gtattacagg ggtcgtcatc actaaacttg
     4201 atggggatac tcgaggcggg gcagcactat cgatccgctc agtgacaggc gcaccaatca
     4261 aattcatcgg ttctggtgaa aaattgactg acttagaagt attccaccca gatcgtatgg
     4321 ctagccgtat ccttggtatg ggggatatgc tgacattgat tgaaaaagcg cagcaagact
     4381 acgacgagaa aaaagcggaa gaacttgcac agaaaatgcg ggaaaattca ttcgatttca
     4441 atgactttat tgaacagctt gatcaagtga tgggtatggg cccactggaa gatctaatca
     4501 aaatgatccc aggaatgaat caagtccctg gaatcgaaaa cgtcaaggta gatccaaaag
     4561 atgttgaacg caaaaaagcg atggtttatt ctatgacgcc agcagaaaga gcaaatcctg
     4621 atctgttgaa cccaagccgt cgtcgccgaa ttgctgccgg ttctggaaac agtgttgttg
     4681 aagtgaatcg aatgatcaaa caattcaagg aatcaagaaa aatgatgcag caaatgtcca
     4741 aaggggatat gaacatccca ggtatggacc aaatgtttgg ttcaggcgtc aaaggaaaac
     4801 taggcaaaat ggcgatgaat cgaatgatca agaaaaataa gaagaaaaag aaaaaacgga
     4861 aataagatga aaaaaacagc ttcggtacag actgaaaatg tctactgccg gagcttttct
     4921 atcagcaatt tttattcttt actaatacaa ttactctaaa cgaagaggtg gcacagatga
     4981 gatgtccgtg gagtgaacaa acagaaagta tgcggcaata tcatgatact gtctggggtg
     5041 ttcctgaata tgatgaccaa aagctgttcc gaaaattgat gcttgatatt aatcaagccg
     5101 gattgagctg gcagacgatt ttaaataaaa gtgaagcatt cgatgaagca tatgatcgat
     5161 tcgatatcaa aaaagtcgca aattatggag aaacaaaaat cagtgaacta atggaaaaca
     5221 aaggaattat tcgtaaccga agaaaaatcg aagcagccgt ccaaaacgcc aaagcagtct
     5281 tgaaaattca agaagaaatc ggcagttttt cagagtatct ttggtcgttt tctaatggaa
     5341 aaatcgttga ttcttcttat cattcacaag aagaagtccc aacaacaagt cctctttcag
     5401 atgaattagc aaaagatcta aagaaaagag gatttaaatt tatcggaagc acaacaatct
     5461 atgctttttt agaagctgtc gggatcatca atgatcacct tgacagttgt ttccgcaaag
     5521 aagaggtcgt acgtatcggc agaaagggcg aataaagcat gcgaaaaatc atattttatg
     5581 gtgctataag tttagatggc tatttggcaa cgaaaacgga tgatcttcaa tggctttttg
     5641 acactccaac aggtaagcaa actacttatg aagcttttta tgaaacaatc gatacgacca
     5701 tcatgggcag aaaaacttat caggaagcaa aaaaatattt ggagaccgaa gcaatttatc
     5761 cagagaaaaa gaatttcgtt ttttccaccc acgcacaatt acaattgcca gatgctgagg
     5821 tcgtaaacga agatcctgtt gcttttctaa aaaatctaca aatgacagaa ggaaaagata
     5881 tctgggttgt gggtggcggt cgattgatca aaccgctatt ggaacatcaa atgattgatg
     5941 aatggtatat ccagattacc cctgtattat taggggacgg gattccttta ttccaaaaag
     6001 gaacatatga agcgcgtttt gaaatgctag acacgacacg attcggtgaa tttatcgaat
     6061 tgcattatgt acgaaagtag aagtaaataa aataaaaaag gcaactatat gcagaaatgt
     6121 cctgtacata gttgcctttt ttattaccgt tatgcttttt actctgttaa tttgttagaa
     6181 aaatctagat acgcatctaa aatcgtttgg aagaaactta atgttccttc aacgatatta
     6241 ccattttcat caattagatt cgcaacgttt ccgatatatg cttccggttg ctgcaaggtt
     6301 ggcacattca aaaagacaag tgattggcgt agatggtggt ttgcgccaaa gccgccgatc
     6361 ccacctggtg atgcagtaac gactaaacct ggtttatgat cccaaacact gtgtccataa
     6421 ggtcttgagc ctacatctaa agcatttttc aatactgctg gcacagaacg gttgtattca
     6481 ggagtaacga aaacgacacc gtctagtccc ctcacttcct cacgaaaacg agtccattca
     6541 gcaggagcct gttctggagt ttcgagatcc tcattgtaaa atggcagatc atcgattttt
     6601 acaaatacac tttcgtaccc ttcaggaagc aatttcgcaa aagcttctgc tacttttttg
     6661 ttgtaagaat cttttcttaa actaccaata aaaaatccaa ttttttttgt catacgattt
     6721 ttcctccttc aaactatcta taataatctt acaaaagata agtaactgga gcaaataaaa
     6781 acacttact
//
LOCUS       DADVXU010000120         6644 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.125, whole genome shotgun sequence.
ACCESSION   DADVXU010000120 DADVXU010000000
VERSION     DADVXU010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.125"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(1..859)
                     /locus_tag="KB953_002533"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="KB953_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="KB953_002534"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="KB953_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBB6867463.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="KB953_002535"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="KB953_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="KB953_002536"
     CDS             complement(2143..2373)
                     /locus_tag="KB953_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HBB6867464.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="KB953_002537"
     CDS             complement(2603..3421)
                     /locus_tag="KB953_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBB6867465.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="KB953_002538"
     CDS             3582..4271
                     /locus_tag="KB953_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBB6867466.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="KB953_002539"
     CDS             4285..5787
                     /locus_tag="KB953_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBB6867467.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="KB953_002540"
     CDS             5800..6276
                     /locus_tag="KB953_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBB6867468.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="KB953_002541"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="KB953_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2172 a         1032 c         1204 g         2236 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atgt
//
LOCUS       DADVXU010000121         6507 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.126, whole genome shotgun sequence.
ACCESSION   DADVXU010000121 DADVXU010000000
VERSION     DADVXU010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.126"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..465
                     /locus_tag="KB953_002542"
     CDS             <1..465
                     /locus_tag="KB953_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HBB6867469.1"
                     /translation="WKMDETYIKIKGKWHYLYRAIDADGLTLDIWLRKKRDTQAAYAF
                     LKRLVKQFDEPKVVVTDKAPSIASAFKKLKEYGFYQGTEHRTIKYLNNLIEQDHRPVK
                     RRNKFYRSLRTASPTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLLGIPD"
     gene            complement(1080..2120)
                     /locus_tag="KB953_002543"
     CDS             complement(1080..2120)
                     /locus_tag="KB953_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBB6867470.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(2469..2795)
                     /locus_tag="KB953_002544"
     CDS             complement(2469..2795)
                     /locus_tag="KB953_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867471.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2782..3585)
                     /locus_tag="KB953_002545"
     CDS             complement(2782..3585)
                     /locus_tag="KB953_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBB6867472.1"
                     /translation="MGDILMARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVD
                     IDPQGNATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAERE
                     FTQVGSEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLK
                     GLIKLSETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKT
                     YIRRTITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3938..4228)
                     /locus_tag="KB953_002546"
     CDS             complement(3938..4228)
                     /locus_tag="KB953_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HBB6867473.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(4364..4543)
                     /locus_tag="KB953_002547"
     CDS             complement(4364..4543)
                     /locus_tag="KB953_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867474.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(4540..4890)
                     /locus_tag="KB953_002548"
     CDS             complement(4540..4890)
                     /locus_tag="KB953_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867475.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(4883..6208)
                     /locus_tag="KB953_002549"
     CDS             complement(4883..6208)
                     /locus_tag="KB953_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBB6867476.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
BASE COUNT         1981 a         1176 c          972 g         2378 t
ORIGIN      
        1 tggaaaatgg atgaaacgta catcaaaatt aaaggaaaat ggcattattt gtatcgagcc
       61 atcgatgcag atggtttaac cttggatatt tggttacgta aaaaacggga cacacaagca
      121 gcctatgctt ttcttaagcg gttagtgaag cagtttgatg aaccgaaggt tgtagtcaca
      181 gataaagcac catcaattgc tagtgccttt aagaaactaa aagaatacgg attttatcaa
      241 gggacagaac atcgtaccat taaatacctg aataatttga ttgaacaaga ccatcgtcca
      301 gtaaagagac gcaataaatt ctatcgaagt ttacgcactg cctcacccac gattaaaggc
      361 atggaagcca tccgaggatt atataagaaa acccgaaaag aaggcactct cttcgggttt
      421 tcggtctgta ctgaaatcaa ggtattattg ggaatcccag attaaatcat agataccgta
      481 agggatttta ttctttattt aaaactttgc aacagaaccg tccgttgcgg gtaataccgt
      541 aacactgaag ggcacgcatt aaggcgtcat cttaatgctt tcctgtagga cactgacgga
      601 gattaccagt ctcccgagcc ttacaggggc ctttcttttt ttgtattttc aattatcaca
      661 atttatcgtc caaaagtgca gaacgaaaat caattcatca caagcatatc agacaattaa
      721 atttaaaaca actaaaaata tacaaaaaag tgtaattgaa tattagcagc actgcctgaa
      781 aagaagtaat atcaggcttt tcagcttggg gaaataagtg aattacctct cttcatgaat
      841 tattataact caaaaaagca catatgcaaa cactagttcc ctattttttt gaatgaaaaa
      901 taactaaaat atctaacgat tatgacttta aaacctacgt ttgcgattgt tttcataaac
      961 ttttaatcta aaaactccgg aataatagct atttatttgt aacatttgta ataaaattaa
     1021 aatcataaaa aagttctaat ttaaaattga tcatttttat agtgatcgct ttttatcaac
     1081 tactctagcc aattttccgt actaactgtt ggtaattcgt taaatgtttc tctttgttcg
     1141 tttgcaagct ttgccgcaat agctttttcg acaaaccaat tttttacata aacgaaaatt
     1201 agattctgca aattgtttac tttttcagtt ttttgttttt gataagcttt cagcaaagtc
     1261 atatataatc ctttgtcagc atcaattcca taagattcca gttcttcata aacaacaact
     1321 gtttgctcta attcttcagc ttttttcttt gcattatgaa tagtttggac ggttttttga
     1381 gcgtcttcgt aagtactaga aaatgttttt atcaagttta atacattttc tggtacaaat
     1441 gtatttatac tgtcctcttt catgatgtcg acgaagttat ctagtaaaac ttgattttgt
     1501 aactgatcag tttcagtgtc tattatgtgt ctattagtgt ctatagaatt agaatagaat
     1561 agattgtctg cgattttcgc aattacggtt gagtccttga tatgactgga ttttgccgta
     1621 tttgcgattt tcgcaattac ggtggtatca ttgatttctc gcggttttgc cgtatttgcg
     1681 attttcgcaa ttacggtggt atcagtattg tcttcctctt ccatgatttt aaagacgtct
     1741 tgattcgtta cttctggctt gcctaagtac aataaattag gcatggattc aatcttaccg
     1801 tcacgtttat ttactcttcc tctcttctgt ttcagaagac ctgctttttc taattccttt
     1861 ttgattttag atactttacc ttcgcggcat tgtaacaatt tcattaattc ttcaactgtg
     1921 aatatgaaaa atatattatc atcagaatct acccagttat ttttgactga ataatcaaat
     1981 cgatctttaa gtagcatata agcaattttt gcatcatttg atagatccat gtatttttcg
     2041 ttagtaaaaa ataccttagg catttgataa tatttttcac gataaatttc ttttgatttg
     2101 taataattaa aatcattcat agccaatcct ctttctgtag gatagcgttt tgttcaaaac
     2161 tatgttacaa tgaattaaac aaatatatct atccactata tatttttcta ctgctgataa
     2221 gtcgccaaac tttgttaaag cagtagtctt tttttatctt tttttagttt accttaaaaa
     2281 ataaattaaa gcaaaccgct ctatttcaag gtttaagctc atagtaacta tatcacgata
     2341 ttacaataat attatatcac gatatcgtaa tattacaata tgaagataag cgacttatca
     2401 gctttgatac tactgatttt tataaaaaaa atatcacgat attatgatat cgtgatattg
     2461 aatgtaaatt aaattttatt ttgtttcttt gctagtttat atccttcaat caagtattct
     2521 tcaggtatgg actttaatag cgcatcttct ataacgtctg gtttactttt gtctgattca
     2581 gcaatagtcg tccaatattt gagtgctgac attagaattg gatttatacg aatgctatct
     2641 gctgttctta cactacttcc ttgatctttt atgactttac ttatattttt cttggttaat
     2701 tcgatttctt ctacttcttt tgatttaaaa ttctttttat ttttatttcc tagtaatcta
     2761 cttactgcag cttgactgcg attatcttct gccatcaaaa tcaccaactt cttttaaata
     2821 ttcttctgta aatgctttat agtcatcttc agctgttgag ggtttattaa aattgattag
     2881 atctgatcca gcagcttgcg cttcgtcaac ggttattgtc ctacgaatat aagttttgaa
     2941 aatttttgca ccaatgactc tagaaatttc ggttgctgca gacgtcattt ctttagaaat
     3001 gttataacgc ttattatact tagtaaatag aactcctcgt acttcaagct tgtcgttcgt
     3061 atattctttt accgtttcaa tagtttcact taactttatt agtcctttta atgagaaata
     3121 cgcagcttca actggaatga taatttcatc agaaacagta aatgcgttga ctgtaagcat
     3181 tcctaaactt ggtggacaat caattataat ataatcatag ttgtccttga ttggttgaag
     3241 tactctttta agcctgtgtt cacttcctac ttgagtaaat tccctctctg cagaagaaag
     3301 ggctatgtcg gctggcaaaa catcaattcc tttatagtcc agaatagcat ctgtaatgtc
     3361 agcctcccct tttaatactt cataaattgt aggttggttt gttccatcta tatcgaaacc
     3421 tacattatct gtagcatttc cctgaggatc aatgtccact aaaagtacat ttttaccgta
     3481 aacaaatttc aaacatgctg ctaaattaac gctagtcgta gttttagaaa ctccaccttt
     3541 ttgattggct actgacaata ttcttgccat taaaatatcc cccattcatc ttatattata
     3601 atataattat atcataatat tgtgatattg caatatcgta tatgcattat attgtaatat
     3661 tgtgtgatta ttttgtttta aagatatata tcggttcgga tataaagatt attcatatgt
     3721 tattcgaaat taaaataatc actttttatt actttaaaat tttttattaa tgataagaaa
     3781 tatgttttgc gagcagcaac tagaataatt taaacattac taaactgtct atattgttac
     3841 taataattcc cttcttatag tgcattttct tagaaaaaaa caatattacg atatcatgat
     3901 attaagatat cgtaatatta tacaattgtg attgatatta tatatgtttt tttatttttt
     3961 tatcatgttt aatcttgaag gaatcatatt ctttttctaa agacaggagc ttttcataaa
     4021 catcagacat gtttaaacta gaggccaggc ttaaatcata aacgaatgaa gcaggtaatg
     4081 attttattct acgttgtcgg gttatccagc ttgcaaccgt tccttgtggg taggcatata
     4141 actcacaaaa ttgttctaca gtgattccta aattcttgat aatgtaaaca ttgatcgggt
     4201 gcgagtaaac aaatgtattg atagacatgg tgcgtaagcc tactttcttt tattattgcg
     4261 tttgcaataa tagtttaaca cacgcattat tctagtatct actcctattt gtaaaaacaa
     4321 aaaaggattc atagagataa ctctatagaa tccgtaaaat accttatata tataacggga
     4381 agatataaaa cctaaaatta tgataacctg ctgcagcttc tacatagaaa gagaagcgct
     4441 caacaccaac ctcatttatc aaacgttggc cataacccca aatctcttct gatagctgat
     4501 caaaggttat atactttagc tcatattgct ggataatgtt cattctaatc gactccattt
     4561 attatcctgg taaaattcga tatgacgtat ttcgtcgtag tgaattttgt cttttccaac
     4621 gtagatcccc agctcatcac tacctaagat cttgccaatg atatcttctg gatagtatcc
     4681 ttcagcgttt cgttcttctt tttgaataga tactgatcta ttttttatga ctgcagcgtt
     4741 caatacttcc gcaatctctt cagtcgtcat ttgttctttt gctaaatttt gactggttcg
     4801 ttgttcattg tcggcatcaa tttcttcagt atgatcagat aaataaaacc ctgcccactt
     4861 taaacgtcca cgatcattgt aatcaatcat ttttaattcc cccatttccg cccgcatgac
     4921 caccaacaag tgtgcttcta gtaatgcttc tagcgccttc taacattgaa gaagcatgta
     4981 caatcgacgt aaaaccatat ttttgccgaa tcttgtcaat aattttatct aaattttctt
     5041 cgtcaatttg cttttgtgga tcactaaata aatccagctg aagcgaatcg gtgtacacaa
     5101 gcttgctata ggttattcca acatgcctta cttcttgtcc ttcataatac ttatcaaaca
     5161 agaaaagaca ataattctgc aattcctttg tattatctgt gatagggatc ttcatctggt
     5221 gtgaaaaccc tggtctcgtt tctaagatcg acgtgccaat gtgtaaatgg acacaacctg
     5281 ttttacagtt atgctgccta atgcgtatcg ctacttgttc cgccatttct tttaccacca
     5341 gctcgatttc atttcttcta gtataatctc gtggtaacac ttgtgaattt ccatatgatt
     5401 tttcctttgt cggttctggt gggattgcta tatctgttcg atcgatccca ttagcatgga
     5461 agtacagctg taagccaatc acacctagtc tattcttaat cgtataagga ttccagttag
     5521 caagatctct aatgctcata atccccatct gattgagtct ttttttcatt cgagagccaa
     5581 ttccccaaaa gtccgtcatt tcaggaatat tccatacttt ttcaggaacg ttttcgtacg
     5641 tccattccgc aacaaatcct tcattatgct ttgcttcgtt gtctaaggct agtttagcca
     5701 aaaggggatt atctccgaca cctactgtag caatcaatcc tagctcttct ttaatattct
     5761 cctggatcat ttgagccagc ttctttctcc gttgacttcg tgttccttca gtcgtaaaaa
     5821 gattcagtga tcgggtcact tttaagattg attcatcgat cgagtaaatc agtagatctt
     5881 catcagccac atatcttctg aaaatattat ttacctgcat atttcgcttg atatataggt
     5941 tcatacgtgg tggaacaacg tgtagtgttt taggaaatgg ttgtggtaag tcacgtggtc
     6001 tactcacatt tgtaatacca taccgctttt ttgcttcagg agaagaagct aatatcaatc
     6061 ctgaaccagt attatcagct cgactcataa caacaagttc tgttgttaat ggatctaaat
     6121 ttctttctat acattcgaca cttgcgtaaa agcttttcat atcgattaaa aagtaatcat
     6181 ttactggctc ttttgaataa tccatcataa ataacactcc attctttcac attatacaaa
     6241 catatgttcg atattgtaaa gaagaaatag ctaaatacat aaaaaaagaa gcataggtat
     6301 gctatcaccc tgcttcttct tttgatttgt atgatcaatt tattctatca ctttactaat
     6361 taatttgaaa atctaataaa aactctcact ctaaaagagt gagagactgc aaattagagt
     6421 acggactttt ttggagttcc gttctttaat attaacaaat gaataaaatc cttacaacat
     6481 ttgagaataa gttatcagta gacactt
//
LOCUS       DADVXU010000122         6363 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.127, whole genome shotgun sequence.
ACCESSION   DADVXU010000122 DADVXU010000000
VERSION     DADVXU010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.127"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(95..1225)
                     /locus_tag="KB953_002550"
     CDS             complement(95..1225)
                     /locus_tag="KB953_002550"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HBB6867477.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1285..1512)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1874..3076)
                     /locus_tag="KB953_002551"
     CDS             complement(1874..3076)
                     /locus_tag="KB953_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBB6867478.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3136..3339)
                     /locus_tag="KB953_002552"
     CDS             complement(3136..3339)
                     /locus_tag="KB953_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HBB6867479.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3481..4713)
                     /locus_tag="KB953_002553"
                     /pseudo
     CDS             complement(3481..4713)
                     /locus_tag="KB953_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4876..5688
                     /locus_tag="KB953_002554"
     CDS             4876..5688
                     /locus_tag="KB953_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6867480.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1900 a         1304 c         1004 g         2155 t
ORIGIN      
        1 aaataatata aaaggtaaat aaaaaagagt ggatttctcc actccatgaa aagccgactt
       61 tttgggacgc ttttttttgc caagcctcac atttttatag ccaaacttct tttgcgactt
      121 gctgcaataa ttggattttc ttccattgtt cttcttctgt taaatggttt ccttcttctg
      181 ttgaagcaaa cccacattga ggactgatac ttagattttc caatggtaca aattgttgtg
      241 cttcttttat tcgttctttt attttctccg tatcttccaa atctggaaac ttagaagtaa
      301 ctaatcctaa aacaacttta ctttctttgt tactccaatg tcttaaagga gtaaaatctc
      361 ctgctcggtc agaatcgtat tccaaaaaga aaccatcata atttgttgaa aacaactctt
      421 ctgcaatggg ttcatatcca ccggaaaaca gccaagaaga agcgtgattc ccgcggcaga
      481 tatgtgttgt gatcgtcaaa tcttctggtt tgtcttccag tgcgttattg ataatctctg
      541 tacaaatatg ggccatttct ttcggatcaa ttcctttttg aagaagtgct tctcttttag
      601 tttgatcagc tagaaacgcc caatttgtat catcgaattg caggtaacgg caaccttttt
      661 cataaaaagc atgaatcgct gctttgtaag tagaggacaa gtcctcataa aaatcagata
      721 gatcaggata aatctccttg atttttgaag aaccatcatt agataggatc tcttgtcgta
      781 aaatcatcgt gggactaggg atcgttgctt ttgcaacaaa agtatcatct gtttccacta
      841 cttctttcag aaaagcaaaa tcttttaaaa atgggtgatt tggattgaaa gataatttat
      901 ctactacccg aatattataa gctggtgctg cttttccgtg gaatgtttga ttatatccag
      961 tttctggaac atagccttcg ataccattta gttcttctaa aaaatcaaga tgccaaaatc
     1021 cacgccgaaa ttcaccatct gtaattcctt tgatgccgac tgctttttgg tcttccacta
     1081 atttttttat ttcctggtct tcgattgcac gaagttcttc ttttgaaagt aagccttctt
     1141 gaactttttt tcttgcttct ttgatcgatt gcgggcgtaa gaaacttccg acatgatcta
     1201 ctgtaaatgg tctatttttg ctcatttcac tcactctttt ctttgttttt tgtaaaaaaa
     1261 aatcctttta ctagcaaata ctagtaaaag gacgattata ctcgtggtac cacctttttt
     1321 cagaaaaaac attctttctc tcagcctgac aataaaatca gtggtcttgg taacgaaaga
     1381 ctgttcgttt ttgcctacct ttcgacaaaa aatcctctaa ggccattttc gacagtcaac
     1441 ttctagcttt ttacaccaac caaagcctct cttgaaccat tttctgtcta ctttccttct
     1501 catcggatac tattagaata aaattagaat agcatgtttg aaagaaaagt caatagggaa
     1561 taagaacaaa caaaaactaa tttgttattc ttagtaattt gtttgtggaa tacaataaag
     1621 cttttctaat atgataattg ttcagtcgtc ctttcatctt agatcacttc cttaaaatga
     1681 tcatacccgt ttcaaccaca taaaaaagga cttaattttg aaaaatttcc catcaaaact
     1741 aagtcctttt tattatagtt atgcaaattt gcttattttc tcgctatttc cgatacatct
     1801 tgaacaggag caattcgact gaaaacactg gtgcaaaagc atttatttgt cttatggtag
     1861 aaccgtggaa aactcagact ggtaataaat tgtcattttt tgaaagaata gcgccctctt
     1921 gaacattatc aatcgcactt accacttgtt gatctgactt tgcatataag tgaccgtaag
     1981 ttcctaaagt gatttgaata ttcgcatggc ccaatcgttc ttgaatagat agcggttgaa
     2041 tattctgctc aattagaaat gcaacatgag aatgtcgtag cgcgtgaagc ttaatcggtt
     2101 tgacacccgc ttttttagca gcttctttta accagttcgc aaaagaataa ggtggcgtaa
     2161 atgttccgtc caactgagca acatattcaa aatttccaat acgtttttgc aagtcatacc
     2221 actgtctgag ttttaaggaa agcttcttcc ccaacccaat cgttcgataa gcacatttag
     2281 ttttgggagt cgaaaagtac caatcatctt ttgtattata aatcaatgtc ttctcaatcg
     2341 ttatcgtgtt tttctcaaaa tctacattcg accaagacaa agcttcaagt tctccaatcc
     2401 gaatgccagt ataaaagagc atttcataca caatcttccg atggtgggtt tgaatcttat
     2461 catccttcaa ttcattcatc actttttgaa actcactgtg ttcccaaaac tcaactttct
     2521 tattctcaga aggaagcgaa gaaacatctt caacgggatt tttagcaatc accccctctt
     2581 taacagcccg atccaataca atttttaggt gccctcgaat ccgattgata taagattttg
     2641 ataacgtttc gccattaact tttttccgtg aattacgtga ctctgtctgg cttagttcaa
     2701 ataaccaatc ctcaatatcg tgacttgtaa tatctcgaac acgaaaatga tagaaaaact
     2761 gcatacgctt ttcgaaaatg aattgcgcat tctcatatgt ttgacttttg acctgtctct
     2821 cgtaccattt tacaaaccta tccttgtaaa actcttcgaa agtcatttga ttggtagaac
     2881 acagttgatt aggaattgtc ttgttaaaat catgtgtttc catgtacttg tcataagcaa
     2941 ttttggcatc tttttgtgaa gcataacctc gtttagtctt ctgaaccttt cttccatttt
     3001 catctatacc tagatacgct cggaagaacc atttttttgt ctttttatct tggtaaatat
     3061 ttttaatttt tggcatcgtt ccattcctcc ctgtacttga gaaaagctac tttaatagta
     3121 ccgcgcttgt actgtttatt ccgcaacttg aatgtgaaaa agatgttcaa ttactcggga
     3181 tggcaccaca ccaacttggc gattattata aaaatcaata ccttcaatcc ttgcaagata
     3241 tgccttgctc tctttaatca ttcgtgcagc ttgactaggt ttaaagccca tctctatcaa
     3301 aattgcacga tttaccatga aatcacattt tgtttccatt ctctcacctc ctgattcttt
     3361 actcctttta aaataatcct ctttataatc tattgtttct cattcgatgg ttgtgttata
     3421 ctcgtcttgt cgaggcggag tacgttgcac aacttgttgt gtaagcagta ctttgctttt
     3481 ttagtagctt gttttctcat ttgatatact ttgtaatttt cgttcataat actcaacctc
     3541 ctcctgataa tactccgggt ctgccttcat attttggaga agtttgatct gtcttggatt
     3601 catttctcct tgttcaataa tcaaaggcaa caaggaagaa tgaaacgttt gatcgacctg
     3661 tgcaataaaa actaatgatg gtgaaacacc atgaattaac caactcatag ttttctcaaa
     3721 actttgagga attgtatcta aagaaatggt gattgcgcca taattgctaa taaaggctgc
     3781 ccatttggca tcaacttttg cacctagatc atttggatca cggtcataaa aacaaattgc
     3841 tccattaagt aactcaaaga acagtttggt taaatcatgg gtttttgaaa taatctttgc
     3901 caagctttgc gctttctctt ttcgataccg tagttcaaag cgattcttta cttttgcctc
     3961 ttctaaagga attcctcgtc gcttccgttg ttcatggtcc ttctgataaa agcaaaaacg
     4021 acactgactc ttctttgagc ctaaatacaa ggtctcacca tttgattcat tgtttatgat
     4081 atccgtaccg ccatgagatt cactcacttg aaaagtggtc cacacatggc ctagtattac
     4141 cttatgctta agttctggaa tgctgagact ccccacaaaa tcatctaaag taaaatcaat
     4201 ccgtgtaaag ttcccctgat aatctaatac cttacgaaaa aactgttgcc aatttgtttt
     4261 tcgtgttttt agccatcttg ctaagtgttt acagccttga cctgaaaact ccatcacagt
     4321 tcctttaacc gcatcgtcaa tggaataccg aatcgtgatt acttgattcc acacgtattg
     4381 gccaatatag ccctttggcg cataatccag ttcctcaaaa cgttcaatgg gcatttcaat
     4441 aatctcttca attagcttta aatgttgatt cgagaaaaag tgaatggtta gataatcaat
     4501 caaaacacgg ttaactggaa cgacctcttc ttgcaaacta tccaacacaa gattcaattt
     4561 ttcttcactt aattgtctcg ttcctttttc tactttcgat aataactatc tcgaaatccc
     4621 cactttttct gctaaatatc gttgtgtcca ccccctttct tctcgagaaa ctcgaatggc
     4681 ttcaccgaga tcatgttgat taacttggtt catcgacaaa cctccttctg ctatttactt
     4741 gtcaaagaac ctattgaact tattttaagt acatttttat tgtacgcctg tatttatttt
     4801 aagtcaatat gttttgactt tttttaagta cacacgtata ctaaatttaa gaacttatcc
     4861 tgaggaggag ttttcatgga gatagacaag caagcagtcg gcaatcgaat tcggcagatt
     4921 cgccaagaat taaagttgag tatggagaaa tttggcaaac taattggtga tttaccgcga
     4981 agtacagtca ataactggga acgtgggatc aaccttccaa aaaccgaaac ccttcaccaa
     5041 atcgcagaga taggtcatgt caccaatgaa tatcttttgt atggtgatca agagaatcaa
     5101 tacattttag aaatgctcca aaaaaaggca ggtaagcttg atcctaagat tgaaggtctc
     5161 atagtagacg aaatcaaaca cgctgacctt gttagtgaaa aagaaatgga tcgaatgatt
     5221 gaatttttta ttacaaatct cattccacca actgaacaag accagtttac tttccaatgt
     5281 atcgatgaaa aaaagcagct ttatcttggt tcaactaatt ttggtaagga agcccagctc
     5341 tatttgcaac atgataatca aaatcatatt ctgcacatga tgccctttac cttctccaac
     5401 tttagtgtgg atcgcatact cgtctattta gcaaatcaaa aatcctattc ctattttggg
     5461 aaacatttac ccaaggaact aggtgagaaa gccatccttc tatactcaat taatcaattg
     5521 accagcgatg tgcgaattgc cccgttggtc tattcagaag aaaccgcttc ttatcagtac
     5581 acagaggaca atcaatatct actagaacag caatatcttt atctaccatt cgtgatggaa
     5641 gtcgagaaag aacgcctgtt aaatgcagcg tatccatcat caaaatgagg tgacaaaatt
     5701 gataagcacg tgacaaagaa aatactgcta ggacactccc caatgggtag ttgtcacagt
     5761 ttcctgttct ttgttagccc cctgttagaa ccgggggctt tattcctgca gtccggctac
     5821 gccttcctgc aatagacaaa actccagatc gggtaattcg cttgtggcga aacccgacct
     5881 ggagtttgtc tctgtatttt gaaccctaaa aattaatcaa acagactgat attctcataa
     5941 tcagcgcgaa ataagccatt aagtacagtt ttcctaccta tttttgataa cccgctggtt
     6001 agttaatatt ccttttccga gagagataaa tgattggtta aatctatata catctcaacc
     6061 tttaaaaatg ttttcgaaat acttcatcag tacatgtcat acaaattacc agtgcattct
     6121 caaattctag ttctttaatt gatttcagtt gaggagcagt tacttttggc ggaaatttcc
     6181 atttttgtcc aaacctcttc ccccgatgaa atttctttgt tgcaattatt gcaatgaaaa
     6241 atttttgttt caaaaatata cataattact tcctccaata aaaactatct acctctcaaa
     6301 ttatttttct ctaataagaa aaacagaaaa ggtaataaga tacaacagtg atgattccta
     6361 ata
//
LOCUS       DADVXU010000123         6315 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.128, whole genome shotgun sequence.
ACCESSION   DADVXU010000123 DADVXU010000000
VERSION     DADVXU010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.128"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..182)
                     /locus_tag="KB953_002555"
     CDS             complement(<1..182)
                     /locus_tag="KB953_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate--amine ligase"
                     /protein_id="HBB6867481.1"
                     /translation="MNAFKTALNNNEMEGKIIGADCSELAPALYMCDEKIVLPKVSES
                     NYIEELIKVIKFFEIK"
     gene            complement(236..2053)
                     /locus_tag="KB953_002556"
     CDS             complement(236..2053)
                     /locus_tag="KB953_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBB6867482.1"
                     /translation="MFILTRRRKIATLVFVDSLLLIAANIAAAKFMKPFVSIPMDLIL
                     ISIGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFSSLSASAASSILILLFINRRYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYVSADSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIHVVGFVDDDPNKRNTYLSGKKVLGALKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESARVKVNTMPSIEELASGKISVSKLKTIDVVDLLGRDEVKLDIESI
                     KDQITDKVILVTGAGGSIGSEICRQIIQFNPAKLLLLGHGENSIYLIDRELRTHHQNC
                     PTEIVPIIADIQDREKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAQGGPITVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSIQKVMDFVKSLPQDDEQLAKDIVTFANASNR"
     gene            complement(2067..2831)
                     /locus_tag="KB953_002557"
     CDS             complement(2067..2831)
                     /locus_tag="KB953_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HBB6867483.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMTASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPDYEEVKKKKFGLF"
     gene            complement(2859..3557)
                     /locus_tag="KB953_002558"
     CDS             complement(2859..3557)
                     /locus_tag="KB953_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HBB6867484.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAR
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILDEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLNKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(3569..4348)
                     /locus_tag="KB953_002559"
     CDS             complement(3569..4348)
                     /locus_tag="KB953_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HBB6867485.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDALGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(4364..5308)
                     /locus_tag="KB953_002560"
     CDS             complement(4364..5308)
                     /locus_tag="KB953_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBB6867486.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTLQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(5354..6133)
                     /locus_tag="KB953_002561"
     CDS             complement(5354..6133)
                     /locus_tag="KB953_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867487.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKNFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISAAYATKAQTVAQQMGYSNISY"
BASE COUNT         1659 a         1313 c         1130 g         2213 t
ORIGIN      
        1 aatttaattt caaaaaactt tattactttt attaattcct caatataatt actttctgaa
       61 actttaggta atactatttt ttcgtcacac atatatagtg cgggtgctag ttcagagcaa
      121 tctgctccaa tgatttttcc ttccatttca ttattgttta atgctgtttt aaatgcattc
      181 actagttcac aacgacgtcc acaacttaat attaaaatat tcaattttta atctcctatc
      241 tattcgaagc atttgcaaaa gtgacaatgt ctttagccaa ttgttcatcg tcttgcggca
      301 ggctcttgac aaaatccatc actttttgta ttgagtaacc tttgatgttt cctacaaaga
      361 ttttctcata aactgcttca tcatttcttt ccttatccaa caatagttct tcatatagtt
      421 tttcccctgg acggatacct gtttcaatga tttcgatttc gtcttcgcta tacccgctca
      481 atcggatcat gtttttcgct aggtctacaa tcttcactgg ttcactcata tctagaataa
      541 aaatttcgcc accttttgct aatgcgccag attgaataac taaacgacta gcttcaggaa
      601 tcgtcataaa gtatcgagtc atacgaaagt ctgttacagt gattggacca ccttgagcaa
      661 tttgttctct gaatactgga atcacactac cgcgagaacc aaggacattc ccaaatctga
      721 cagctgagaa tttcgtacag ccttcttcat tcaatccagt cacaatcatt tcagcgattc
      781 gttttgttga acccatgaca tttggaggat tgttagcttt atctgtcgat accatgacga
      841 agtttttcac ttttgctgct ttagctgctt ctgctacatt ttttgtaccg aaaatattgt
      901 ttttgactgc ttcttttgga ttatattcca tcagtggtac atgtttatga gcagctgcat
      961 ggtagacgat atctggatga tattgttcca tgatctcatt gattttctcg cgatcttgga
     1021 tatctgcgat gatcggtacg atttctgtag gacaattttg atgatgcgtg cgtaactcac
     1081 ggtcaatcaa gtagatcgag ttctccccat gtcctaaaag caatagcttt gcaggattga
     1141 attgaatgat ttgccgacag atttctgaac cgatcgatcc tcctgcgccg gtgacaagaa
     1201 tgactttgtc tgtgatctga tctttgatgg attcaatatc cagctttacc tcatctcttc
     1261 ccagtaaatc cactacgtca attgttttta attttgatac gctaattttc cctgaagcaa
     1321 gttcttctat agaaggcatc gtgttgactt tcacacgagc ggattcgact aactcgaaga
     1381 ttcgtcgtaa tttttttctt gataaggaag ggatagcgat cgttaccatt tgaatgtcat
     1441 atttttcaat caattccgga atatctttta atgcacctaa tacttttttt cctgaaagat
     1501 acgtatttct tttatttgga tcatcatcta caaacccgac aacgtggata tcttgagccg
     1561 ttttggatcc aagaaagctg ttgtataaaa tacgcccgcc ttcgccagca ccaacaatca
     1621 atgtattttt agcagaatct gctgatacgt atctcatatt ttttgtttcg atgtagatac
     1681 gccagatcaa tcggctaccg atgatcaata agagcgaaag cagataagca aagataacta
     1741 accgtaaact gtatcgccga ttaatgaaca gcagtatgag gatacttgag gcagcacttg
     1801 cagatagtga actaaaaatt gcaatgatct ctcttaaatt tgtatagcga ttgatccgag
     1861 tgaaaacttt gaataaacta ccataaaata aataaaaacc aatcgacaaa ccgatagaga
     1921 ttaaaataag atccattggt atagatacaa acggcttcat gaatttagct gctgcaatat
     1981 ttgcagcaat taataacaag ctgtccacaa aaaccaatgt ggcaattttt cttcttcttg
     2041 ttaatataaa cactttttaa ttccccctaa aataaaccaa acttcttctt ttttacctct
     2101 tcatagtctg gataactgat ttcatccccg ttgatcagat cctttgcaac ttgcttcatc
     2161 tgtactactt tttccttacc aaagtccttg gcaatctgct catagcactc ttttaagtaa
     2221 aaggtgcgct tagagactcc gtgtgcatca gaagcagtca tctgcacgag attatgtttg
     2281 accattgttt tagctgtttt ctgaatatct ttgccaaata ccccaacata acttggcgca
     2341 gtaagttggg ccaaacaccc catatctagg aacggaagca aatgattcgg gtcttcgcga
     2401 aaatgcgcgt tgcgttctgg atggacgatc actggtgtct ttcctagttc taacaactca
     2461 aaaaaaagcc gttctgcata taacggcact tccagtgtcg gaaattcgat cagaagatat
     2521 gtatcatcta aatctgtaaa taaaatttca tctcggtgga tgtcttcgat gagcgttccg
     2581 gtaatacgta cttcttgtcc ttccaataac gtcaaaggca actgtctttt ctctaattcg
     2641 gcttgtaacg aagcgactag agaaatgact tgtgattttt cattgctgta ttttccatta
     2701 ttgtggtgtg gtgtgcataa aatatgcgtg atgccttgct gaatcgcttt ttcagccatt
     2761 gcaatacttg cttctaagtt ttctgccccg tcatcgattc ctggtaggat atggcaatgt
     2821 agatcgatca tttctcttgt ctcctctctt cttattcctt aatttccgta gtaataacca
     2881 gcatctttgc tatgttctgc tccgttgtat acaacaccta aaactcgtgc ttgtaccatc
     2941 tctagcagtt cttttgcttt attcaatgat tctttacgtg atgtattttc acgtacgacc
     3001 aataaagtgc catccgtttt tgaagacatg atctgtgcat ctgttacggc tactactggt
     3061 ggcatatcga atatcaccac gtcaaataac tgtcttgctt cgtctaggat ctgatccatt
     3121 cttggcgaac ttaacaattc tgatgggttg ggtggcttag gtccactagg caaaatcgac
     3181 aagttctcga ctggcgttcg ttggattaca tctgctacac ttcctgatga actaagtgct
     3241 gtacttaatc ccctggcatt gttcagctgg aatgtcttat agacgacggg tttacgtaaa
     3301 tctgcatcga ctagtagaac tcgttgaccg gatttagcaa aaacgactgc aatgtttgct
     3361 gcagtggtgg attttccttc tccaggacct gatgaagtca cgacaatcgt tttgatttgc
     3421 tgaccagctg cggaggcaaa ctggatattc gtccggatcg tacgatattg ttcggcgatt
     3481 ggtgaagatg ggtttgttaa tgtgatcaaa ctgactgcat gagtttgtgc tgctttttgt
     3541 ttctgtgttc gtgccatttt tcttcctcct agactcttga gcggcttctg cgtgatgggg
     3601 ctgttttatc gtttgtagct tttggtcgtg caggagctgt tcctttttta cggataccag
     3661 cgttcaattc tttcgctgac atttctggaa ctgttcctaa gattgggaat cctaatgcat
     3721 ccgtcacata tttttcatct ttcactgtcc gatcaagtaa ttcgaataag aatgccagac
     3781 ctacgcctac catcattcct aatactaagc caatcactag attcaattta ttatttggcg
     3841 aaacaggtgt cgtattggca actgcacctg aaataatcga gattttatcg acattcatca
     3901 cgtcttttgc gttctcttgg aaaacttgtg aagttgtgtt agcgatctgt tgagcagtca
     3961 ctgcattcgt gcttattgcc tggatcgaga acatttgtga attttgtgac tgattgacgg
     4021 agatcgcatc tttgatttgt ccagcagtca ttttcacatt gtcttctgtt tctaaacgat
     4081 ctttgacttc atttagtact aaatcactga cgatcatatc tttatacgta ttgatcattt
     4141 gcaggttcgt attgacatca ttgacatttg ctgtgtctga ttgtggcagt gtcacgatca
     4201 attgtgcttg tgaactgtat tttggtgtca agatgaaaaa tgttacgatt cctgagatgg
     4261 ccaagccagc aaacattgag atgatgattg ttaccaagtg tttgcgtaat gtttcaaaaa
     4321 tttctttcaa gctgattgtc tcttccattt tttctccact ttcctattta ttctctagat
     4381 tcatttgatc ttttaattct tttttcacac gatcaagttc ttgatcgctg actttttgat
     4441 aggagatatt atcttgcatg aagccttctc cttgcagctg atcttgcttg atcgtaccaa
     4501 atgcctcccg ataatctaaa gcgatttttt tcatttcatc aaagctcatg tctgttttca
     4561 tattcgactc tagagcattc aaaacggatt gatagttgct gacaccgttt aaactcaata
     4621 ctttttttgc tacaccttct acgatctttc tttgacgctc ttgtctacca tagtcaccat
     4681 ttggatcttc ataacgcata cgagaataag ataatgcctg atccccgttc aatgtaattt
     4741 taccgattgg aaagtcatag ccatcttgtg agaaagtgag gtcattattg acttcgatac
     4801 caccaactgc atcaacgagt tcctttagcc ctttcatatt gatagagaca tagtgatcga
     4861 ttgggatgtc tacatatttt tgtagggtat ccatcgccat ggcagctccg ccgaatgcgt
     4921 aagcgtggtt gatcttgtct tttgtgtcac gacctacgat gtcgacatac gtatctcgag
     4981 gaatgctgac gatcgttgtc tgtttgtctt caggactgac tgtggcaacc atcatcgtat
     5041 ctgatcttcc ttgatcgacc cgacctaaat ctccagtatc aatgcccatc aatagaacag
     5101 agaaagattc ttgtttgtcc agatcaactg gtttcgttcg ttctccttgg gttcgttcca
     5161 ctgattcgta agttttctca atagaatgtg agagatccag atataatttt gctcctactc
     5221 ctacaattgc taatactaat acgataagaa ctgctaaaat aatcagaaat atttttttcc
     5281 ctcttgattt tcttcttcgt cttgccatag tttcctccgt ttgctaacct tgcttgaatc
     5341 aacgttttaa ctgtcaataa cttatattgc tatagcccat ttgctgagca acagtttgtg
     5401 cttttgttgc gtatgctgcg cttatttttt cattccggat ctgctctatt tgactgagcg
     5461 ccgtcaaata ttgatcgtaa gtagcggtgg cagtcacttg accatccttc aacatcgtat
     5521 cgaataactg atctagttgc gtgacttgcg cgatctgagt atcagcgaat cctagatatt
     5581 gcataacaac cgttttccaa ggactatctt taaaaaagct catattttca cgtacacgag
     5641 cgacatctgc acttgtcacc ttttctttga tgacgacatc gtctacagct tgctgccagt
     5701 ttgatacatt tttagtaaat aaatcgttga cagctgtctg gatcttcagt tttgattctg
     5761 cactatctaa ctgtgtcacg attgctttct tctcatcttg gattgcaaca gcttctttag
     5821 gcaattctga ttctttgatg tcgtaatcat ctgctgaaag tttgacagag gatagcttat
     5881 ttgacaaact tgtgattgtt tcttctgtta tttcagggtt tagaaaattt ttttcttttg
     5941 tactatacag cttgtctact tcttttgata aagaagtcaa ggtgtctttt tgattggtga
     6001 tggcagattc tgcataattg atccgttgct gatgttgccg ctgattattg atatggtaac
     6061 caaacaaaca gatcgcaata gcacctgcac ctaccgccac agcagtcaat gacttgacga
     6121 atggcttttc catagattca ctcctaagta ttgatgatag ataaattaag atataattca
     6181 catacataaa aatgacagct ttttatttta aaaaacataa ttaaattaat atattcacag
     6241 aaatatttgt aaacaagttt tccctatata atcataccac aaactatttt cttgactagg
     6301 gcaatttacg attta
//
LOCUS       DADVXU010000124         6224 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.129, whole genome shotgun sequence.
ACCESSION   DADVXU010000124 DADVXU010000000
VERSION     DADVXU010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.129"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(207..1169)
                     /locus_tag="KB953_002562"
     CDS             complement(207..1169)
                     /locus_tag="KB953_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBB6867488.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="KB953_002563"
     CDS             complement(1171..2610)
                     /locus_tag="KB953_002563"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBB6867489.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="KB953_002564"
     CDS             2795..4741
                     /locus_tag="KB953_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HBB6867490.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            5005..5877
                     /locus_tag="KB953_002565"
     CDS             5005..5877
                     /locus_tag="KB953_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBB6867491.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT         1986 a         1061 c         1107 g         2070 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaggc acatttaaaa ttgatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat
     4801 caaatatttg agaataacat tttatttaat cttttcatat atcaattgta tgaaaaatta
     4861 ttagaatttt aaaaaatgat aaaaattcaa aaagaaaaaa tagtagttat ttacttgtca
     4921 tgttactaaa taaaaaaata atttaaatat gatatatagt catttattta gtgatttcat
     4981 tctaaaataa agggagaaat gtctatgcta tacggtgcaa ttgaagcagg cggcacaaaa
     5041 tttgtgtgtg ccattggcga tgaagaaatg acgatcaagg aaagagtgag ctttccgaca
     5101 acgacacctg aggaaacaat gcctttagtc attgacttct tcaaacaata ccaagcagat
     5161 ttagcaggga ttgggattgg ctcatttggg cctatcgata tccatcgcga ttccgccact
     5221 tatgggtaca ttacctcgac cccgaaattg gcatggcaaa attttgattt catcggtacc
     5281 atgaaaaaag aatttccgat cccgatttct tggacaacag atgtcaatgc ggctgcctat
     5341 ggtgagtatg tcttcggcag tggcaaagga ttgagcagtg tcgtttacta tacgatcggt
     5401 acgggtgttg gcggcggtgc cttacaagaa gggcgcttta tcgaagggtt tagtcatcca
     5461 gaaatggggc atatgctcgt cgtccctcat ccaaaagatg actttgctgg cagctgtcca
     5521 tttcatggca actgtttaga agggatggcc gctggacccg ccatcgaaaa ccgcttaggg
     5581 aaaaaaggac aagaggttgc agaagatgat ccttattggg aaatcgaagc ctcctatatt
     5641 gctcaatgtg cctacaatac aaccttgatg ttttctccag atgtgatcat ttttggcggt
     5701 ggtgtgatga aacaacgcca tatgctgcaa aacgtccacg acgcatttgc aaaattggtc
     5761 aatgggtatg tcgaaacacc tgagttaagc aaatatatcg tgaccccagc attagaagac
     5821 aacgctggaa cgctcggttg tttggcattg gctcgagaag cggtcttaca ttcataaacg
     5881 aaaaaaggct cttcgacatt tagtgttggt agagaaagat acaactgaaa atttagtcat
     5941 aaccagttag gcagagtgct taactggttt ttgttttact tggaagatca agccttgaat
     6001 gagataaatt cttgctcgaa agttatggaa atttcgatag ccgtaggcca cttgtattgt
     6061 gcaatagttt tttacaaaaa aaagggggtg cggatatttt tttggacctt tttaacaaat
     6121 aggttctgtt gcaaagtttt aaatctacta tcagataagg tagaataata gaaaaagata
     6181 gcaggaggaa tgacgatgag tcattttaaa ggaaagcaat ttca
//
LOCUS       DADVXU010000125         6173 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.130, whole genome shotgun sequence.
ACCESSION   DADVXU010000125 DADVXU010000000
VERSION     DADVXU010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.130"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            725..811
                     /locus_tag="KB953_002566"
     CDS             725..811
                     /locus_tag="KB953_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HBB6867492.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="KB953_002567"
     CDS             complement(932..1621)
                     /locus_tag="KB953_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867493.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="KB953_002568"
     CDS             complement(1628..1906)
                     /locus_tag="KB953_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867494.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="KB953_002569"
     CDS             complement(1866..2780)
                     /locus_tag="KB953_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBB6867495.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="KB953_002570"
     CDS             complement(2762..3142)
                     /locus_tag="KB953_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HBB6867496.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="KB953_002571"
     CDS             complement(3592..3879)
                     /locus_tag="KB953_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBB6867497.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="KB953_002572"
     CDS             complement(3899..4123)
                     /locus_tag="KB953_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HBB6867498.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4781)
                     /locus_tag="KB953_002573"
     CDS             complement(4578..4781)
                     /locus_tag="KB953_002573"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867499.1"
                     /translation="MAAMEMKKEILMTKKHLLFGSILLGILSFSPMLLSVANKGNKKG
                     ELKLNPNYYNSQNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="KB953_002574"
     CDS             complement(4763..5296)
                     /locus_tag="KB953_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HBB6867500.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="KB953_002575"
     CDS             complement(5317..6159)
                     /locus_tag="KB953_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBB6867501.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DADVXU010000126         6113 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.131, whole genome shotgun sequence.
ACCESSION   DADVXU010000126 DADVXU010000000
VERSION     DADVXU010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6113
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.131"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            56..901
                     /locus_tag="KB953_002576"
     CDS             56..901
                     /locus_tag="KB953_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBB6867502.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1182..2402
                     /locus_tag="KB953_002577"
     CDS             1182..2402
                     /locus_tag="KB953_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HBB6867503.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2399..5119
                     /locus_tag="KB953_002578"
     CDS             2399..5119
                     /locus_tag="KB953_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBB6867504.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5103..6047
                     /locus_tag="KB953_002579"
     CDS             5103..6047
                     /locus_tag="KB953_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBB6867505.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         2284 a          961 c         1195 g         1673 t
ORIGIN      
        1 gtgagaaatt tagacttaca ataagcagtg aatttataat caacgtatag gagacatgga
       61 tgaaatgaaa ctaattggta tagatttaga cggtactctt ttgaattctg accagagcat
      121 tagtcaaaaa aatgcaagaa caatgaaaca tttaccggaa gactgttttc cattcatttg
      181 ttctggaaga gaagtagaag acattaataa tattctcaaa aaatctggtt taaggatacc
      241 agcagtaggg ttaaatggtt ctgttggatt tgatggagaa aaaaagatat ttgagtcttc
      301 cttcgataaa aatgatgtaa aaaaaattga ttctgtccta tcgaaatttc caaccaaaat
      361 atatacaaat ctcggaagtt atgaatctca gaattataaa aataaaatga aaaaaatttt
      421 tcacgaggta gggagtgaat tttctatcaa agagctaaac tatgagcttg agtatgaaaa
      481 atctatttgt tcaatccctt ataataatat tgaggaaatt atagaaaagg atgctatcag
      541 catttacaaa ttttttattt ttattcctaa tcgtcaattg aaagctaata tatttaacca
      601 tttgaaaaag atgctaaatg tgtctgttac tgaatcggca cctgtaaata ttgaaatcgt
      661 acctcaaaat gtatctaaag gtgtggtgtt tgatcgtttg gaatcaatct acaatttaaa
      721 gaaacctttg agaatcgcga tcggagatag tttaaatgac ttatctatgt ttgaaagtgc
      781 agatattagt tttgcaatgg gaaatagtca tcaagaaatc atgaaaaaag ctgattatgt
      841 cactgtttca aatgataaag acggagtatc tcaagcattt gaaagaattc ttaccatata
      901 aactaattaa ggtagtatag aacatagaat tctaaatacg gaaaaatgga gcataaattc
      961 cagtacaaat agtacttctt cagtgtcaaa cgaagcgaat ataacataga tatttaggat
     1021 tgtgacaaaa atcatgtcac aatctttttt tcgaataaac ttattttttg aagtcggcca
     1081 tttatcgtaa tttctttgca taatacgaac aaattttcgg ttattgatga aaaaaaacat
     1141 tgaaaatggt atgataaaag aaaaaaagga ggaaattttt aatggtgaaa tttatccatg
     1201 cggcagattt acatatggat cggtcatttg aaggactgac aactcttgat aaaactgtac
     1261 aggaaaaact attgaagacg aatctgaccg ttttatcaaa tatcattgaa caggcaataa
     1321 taaataacgt tgattttgtt ttacttgcag gagataattt tcatcaaaat cgtccttcat
     1381 taaaaataca gaaacatttt tcagaacaga tgaaacggtt agaaaagaac catatacctg
     1441 tatttataat attcggaaat catgatttct atcaaaaaga gcggtattgg ttctcctttc
     1501 ctaaaaatgt ccatttattt acatccgaaa cggtagagac aaaaaaaata acgataaaaa
     1561 gtggagaaac tgtctctctc agtggattta gttatcgtca accgtggatt caaaaagaca
     1621 aagtgatgga atttccttct agagaactca cagattacca tattgggttg taccacgggg
     1681 aacctggcgt ctctcaaaaa ggaaactatg ctcctttcca accctcaaag atgcaagaaa
     1741 agggatacga ttattgggct ttagggcata tccatgtgcc aacggtctta aatgaaaagc
     1801 aaacaattgt ttatccaggt gcgcctcaag gtcatactaa aaaggaacaa gcttctacat
     1861 ctattctact agttgaatta agtaaaggaa gttgccaaat acatccgatt aaagtagcag
     1921 aggtctattg gaagacaaag gaaatatcat tgaggaaagc aagaacgaca aaagaggtca
     1981 ttgttcacat tcgtcagcag ctttcaaaag tagaggatgg atttagttta attgagataa
     2041 aattaaaaga ttatgaccat ctcaatacag atgttttgga acggatacaa tcaggagagc
     2101 tattagacta tttgttagag gaattctcag ataggataaa tgatttcttt atttggcgta
     2161 tttctctcat agagaatact ttttatacga agaccccact tgctgcttct gctaaattga
     2221 tggaacagtt atttcaatac tatcagacac ctcaagattt ccaacaaatt ttaaatgaag
     2281 tatatagtca tcaagaagcg gctagaatat tgagcgaact gccggaatat caagaagaaa
     2341 cacttgaaaa agccaagcag ctgcttaatc aagatttttt atttgaggag gatcaggaat
     2401 gaagatttta aggatcgaga ttgctgcttt tggaaaatgg cgtcaaaaaa gttttgactt
     2461 ttattcaggc aatcagctga tctatggggg aaacgaagca ggaaaatcaa cgatttatca
     2521 atttattcaa gcaatattat ttggtttccc ttcaaaaggc cgtaaaaaaa aggattatac
     2581 accaaaagac ggctcagcct atggcggcaa aatatggctt aaacatcctg tatatggtga
     2641 atttgctgtc gagcgctaca agcagcaaaa tcgcggaaaa tcgaaggtgt ggctggggga
     2701 tcaagtggga agtgatgagt tactagaaaa actgatcgca cctttgacga aagagttatt
     2761 ccagcaggta tttactttcc agcaagagca gttgatggaa ctagatcatt tgagagaaaa
     2821 agagctacat gatgcgctga tttctttagg catcacgggc agttctcaag tattccaaaa
     2881 gcggcaagaa tattatcaga aagcacaaca attattcaag aaaaaaggcc aaaaattact
     2941 aatcaatcaa aaattaaatg aatggaaaga gctgcaagaa aaaattcatc aaaaacaaga
     3001 acaggaagct tattttggca agttgatcca acaagaacag gaatacgagt cgcaacagca
     3061 gcagttattg gcacaattaa aagaggcgaa caaacaattt tcattagcca gacagcaaga
     3121 acttaatttt tctttatatg aagaatggca atctctcaaa aaaaacagca ctacaggtct
     3181 tcctgatgag caacttcttt tagatctgga gtctttctcc aaacggtatc agcaaacaaa
     3241 tgagcgtttg gaagaattag aagcaacact ggaaaagcat agcgggatgg atcagcagtc
     3301 agccagatat tatttttatt tagagcagga gcaggaacta aaagatctcc agcaacttca
     3361 atttacagcg gaacaattaa acgaagaatg gcaaagatta caaattgaga ctagagaaac
     3421 aaattcagaa ttgaagattt tggaacaaag gtggcattgg cagaaaaatc cgttaccgga
     3481 agaattttta tatgaagaag aatggaagca tcttctggct gaagaaagaa ggattgctga
     3541 tctagaaagc cagatttctg tgcgtctcca aatagctaaa gaacagcaag agatgattga
     3601 agcagaagtg aatgacttgg aagagaccta tccagcatta ttacaaaatc agtcagagat
     3661 agcaccagtc tcttcatcta aaccgtatat gctgatggct ggcggagcaa taatcattgc
     3721 aggtatattt gtaccaatgc ccttgaaagt gatcctttta ctaattggtg caggcatgat
     3781 tggctatttc ttctacaaaa ggcaaccaaa aattaaaact aaaaatgatt tctctgagat
     3841 aaaaggaatg tggcaagaaa aactaggaca acttgatcta gccttagctc aattagctga
     3901 agcagaagaa aaacaaaaga gaactggaga agaaaaacag attcttcagc aagaagtata
     3961 tcgttttgcg gatcatcggc acttaggaaa gatggatacg ctcaaactca tgaaagaata
     4021 tggacaggaa gtcatagatt atcagaaatt atatggcgta tttgagcgga aacataaaag
     4081 agaacaggaa atacaagaaa aactacgaca aatcgaccaa aaatttgagt ttgttaaaga
     4141 atggttaccg atacaagata agacaatttc agaaaaaatg gtattgcttt cccgttttat
     4201 acaagagatg gaacagatga aatttgccag aagctatcag caaaatactt tattgaagca
     4261 agaaatcaat caattgaaaa agaaacaaaa agtatggata caagaataca gcgaacaatt
     4321 gactcaagca ggcttatctt atccttcgga agtacctgtc tttttacaaa aagccaaaca
     4381 agccgaacag cagatgaaaa gatacaatga attatcggaa atgcttactc cactatttcc
     4441 tgaagagctg acaaaagaga aattatctgt aagactgcgc caactgaaag aaaagcaatc
     4501 ccaactccag caagaagaaa aagaagcaag tgaaaatcgg caacgactcc aacttcaagt
     4561 agaagagttg caaagagacg gcacattgga cgaactttat caacaagcta gccggcaaaa
     4621 agcaaaatta caggaattac tggaggaatg gggagcactt gaaatcgccg ggacattctt
     4681 aggtgatctt tcaacagaga tgagtgaaag acaattaccc cagttgttaa gagcagcagg
     4741 tgattattta ggtattttaa caaaaggaaa ttatcagaag atattactga aaaatgatac
     4801 actgacttta acagacggat atacagaatt tcccatctat gagttatcta caggtacgaa
     4861 agatcagctg attatggcga tgagatttgc ttacctctca ttagaagcta accgttcgat
     4921 atgcccgatc atcattgatg atggctggct tcattatgat catcagcgaa agtatcaatt
     4981 agctgaactt ttagctaaat ttggagaaaa gtatcaaata atttgttttt cttctgataa
     5041 agaaatggta agctattacc atgagatgaa ccaaatcgta aaacaattag aaggagcaaa
     5101 taatgaaaaa aattcgtgat ttagtaatcg atgagcagtt tgaacaattt gtcctgatca
     5161 aaagtgcaga tgtaagaatc gctaaaaacg gaaaaaaatt tattgctttt actttccaag
     5221 atacttcagg tactatcgat ggtaaatact gggatgcttc ggaggaggaa atcaataaat
     5281 ttacagccgg cacagtcgtt tttctaaacg gtaaaagaga ggtttatcaa ggaaaccctc
     5341 aagtaaagat tcttcatatg cgcttaacaa gggtagatga gccaggagag cctagtttat
     5401 acatggaaag agcccctttg aagaaagaag aaatggaaga agaaatcaat caattgttgt
     5461 ttgagatcac caatgcgcat tggaatcgta ttgttcgcta tctacttaat aaatatcaaa
     5521 aacaattttt tgagtttccg gcagcaaaaa gaaaccatca tgcatttgcc agtggattag
     5581 cttatcatac tttgacaatg cttcacttag ggaaatcaat cgccgatgaa tatcctgagc
     5641 tgaatcgagc attgttgtat gctggtatca ttttgcatga tttaggaaag gtgattgaac
     5701 tttccggtcc tatgtcgaca gaatatacgc ttgcaggaaa tttgatcggt catctggtat
     5761 tgattgacga agaaataact aaagcctgtc ttgcgttgaa aatcagcgaa gctgacgaag
     5821 atgttctcgt attacgtcat atggttttag cacaccatgg cttattagaa tatggctcac
     5881 ctgtacgtcc aagaataatg gaagcagaaa tattacatca aatcgacaat atcgatgctt
     5941 cgatgcagat gatgttgact tcgattcgtc aaacagaacc aggaaaatac accgatcgga
     6001 ttttcggtat ggacaaccga agtttctatg taccaaaaga tttatagcaa aaatttattt
     6061 ttttcattca aaacaaaaaa agcgagacat aagtcttcgc tttttttgtt ttc
//
LOCUS       DADVXU010000127         6087 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.132, whole genome shotgun sequence.
ACCESSION   DADVXU010000127 DADVXU010000000
VERSION     DADVXU010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.132"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..1331)
                     /locus_tag="KB953_002580"
     CDS             complement(<1..1331)
                     /locus_tag="KB953_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6867506.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRT"
     gene            complement(1629..2564)
                     /gene="murB"
                     /locus_tag="KB953_002581"
     CDS             complement(1629..2564)
                     /gene="murB"
                     /locus_tag="KB953_002581"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBB6867507.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(2637..3557)
                     /locus_tag="KB953_002582"
     CDS             complement(2637..3557)
                     /locus_tag="KB953_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBB6867508.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            3670..4422
                     /gene="xth"
                     /locus_tag="KB953_002583"
     CDS             3670..4422
                     /gene="xth"
                     /locus_tag="KB953_002583"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBB6867509.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            5286..5819
                     /locus_tag="KB953_002584"
     CDS             5286..5819
                     /locus_tag="KB953_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HBB6867510.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT         1829 a         1169 c         1022 g         2067 t
ORIGIN      
        1 gtacgagaag tgattcgact ctcaatcaca atattgtaat ttttaaatcc tttcacttga
       61 gaaataatgt aatcggtata agcagatcct tgcgaaaaat caatacagac atgaatcggt
      121 gcttcaaaat atgataccgg acaaataccg accaataaaa agagataatc ataaaataag
      181 cgaatcatga aacgttcatc ttttcccaag aaatagcgtt tattctcttg tatgaccttt
      241 tggattgctg tactgaatat tggatacgtc tcgaagaaaa actgtagctg ctttttagac
      301 gtaaagctag tcatctcaaa gtcgaatatc caattttttc gattgacatg catcataccc
      361 atacgaagtt cttttcgtat atatagtctt tcttcttcag agatttcaat cgaaaatggt
      421 aattgatcaa tcaattccac tgacattttt tgactaatca tgtggatatc atgaaaatga
      481 gcttccgcaa gtccaaatgc gccatctaat tcaaattctt cttccgtgat caaaaacaac
      541 atgatataag aaatctcatc aatgatctgc tctttaggga tatgaagaaa agaaatatgt
      601 gataaatcct tgatgatctt cattccttct gagctcttaa agtattcatc tgaaaagaaa
      661 cgttttccga ttggttgatt cccataggcc ctcaacaaac taatagtggt tagcaaattg
      721 aatttgattc gttttgttgc agacagtttt aaattgaaca gatattcaaa atattgcacg
      781 atactctttg cttcattcat gacgctttct ctaaatggca tagaaaaacc attaaagacc
      841 ccgaaatata ccccaaataa tacgttccgt atgtttattt cttccccaac taatatgttt
      901 ttctttaact ttatttgatg ctgttcaaga aaagagacta attctcttct ttttacatag
      961 gctcttgata tgcttaaaaa atgattctct gcaaattttt cgatcgttac ttttgaatcc
     1021 acgatctccg ttaacagtcg agcaattttt gatcgttgaa agtagattct tcttaaattt
     1081 ttgatatccg tcaaattcag acgttcgaca tggatctctg cttcttctga aatttggatc
     1141 tctggctttg ttttgaattc agtaaattct ttatttaatt ctatgatata atttcttgtt
     1201 ttaaatttag acaaacctaa tgtttcatat aacttaggca gcactacata attctttttc
     1261 tcaaattcta aatatctagc tagcagcagt ttttctctat cttcctcttc tataaggtaa
     1321 tccaacttca tatcctacac ctcaaaaaat gcctcatacc aattacttga tatgcagatg
     1381 aaagttcatc ttttcaccta tactgatttt atgatggatt atgcttgttt tttcatcagt
     1441 tattctcatt ttttatcttt tttttgagaa tattgcaaaa aatggaaaaa tttatgagga
     1501 aatattctat attttttgtt ttattgtgtt aaaatgtaca agatttgcgc catttgcgta
     1561 aacaaccaca aaaaagagca ggacggccac cgtcctgctc ttttacttat tttaagttcc
     1621 attacttatt aattggctaa acttctgctt gtttctgata ttgcttgttt ttctccaatg
     1681 atccgcactt ctgtttctaa agacacatca aatttttctt tgattacttc ttggatgtgc
     1741 gcaatcaatt caatataatc tgtcgctgtc gcatgatcga tattgacgat gaatccagca
     1801 tgtttttcag agacttgtgc accgccccat ttcaggcctt gcaatcctgc ttgttggatc
     1861 agctgacctg tatagtgtcc tactgggcgc ttaaagacac ttccgcatga agggtattct
     1921 aatggctgtt ttgattgacg caattcggtc aactcatcca ttcttgcttt gattgcttca
     1981 taatcccctg tttgcaaact gaatcgagct tccaatacga tagcacgtaa ttcttggact
     2041 ttactgtgac gataagaaaa taccatttcg tcttttgtca aagttttcaa tgttccatct
     2101 tcaagcaaaa gatccacttc agcaaaaaca tctttgatct ctccgccata cgcacctgca
     2161 ttcatataaa ccgctccacc gacacttccc gggatgccgc aagcaaattc aaatccagtc
     2221 aatgattctg ccaaagctgc ataagtcgta tcaatcagtg aggcacctgc ttccgcaatg
     2281 accatcgttc ctttgacatg aatctgtttc atttcagtca gcatgatgac tacaccgcga
     2341 atcccgccat cttgaacgat cagattgctt gcatttccta ataccatcca aggtatatca
     2401 tggattcggc aataatcgat gatttgcttt acttcttctt gttttttcgg gaatgcaagg
     2461 acatcagctg gtccacctgt tttcgtaaac gtatagttca tcaatggttc atcaaacaat
     2521 atagtaatcg tggctaagct ttcaactatt tcttctttat tcactgagga ttttcctttc
     2581 ttttctcaaa cgtcctcatt attctatcac gcttaataca atcttgccag tcttgttcaa
     2641 agttgatgac ggtagtgatc tttaagcact tcctcacata attcatgact tcttacaact
     2701 tctttttgtt taagcaattc tgtttccgat ttacctaaac ggatctgaga aaagaaggcc
     2761 tctaccattg gctcaaaccc gcgtttgact agcgtgtttg tccagtcgcc aaattctttc
     2821 actttttttc catcagcatc ttgaatgaca agctgggtca gatcgtttac ttcataagta
     2881 ccatttttac ttgttacttg atatttttct atattagcgc cacttgctaa atccatcgtt
     2941 aaaatagccg tttgattttg tgtttctaat tgtaaaatgg catattccaa aaattccttt
     3001 gtttctctta tctggtattt tgttctcaaa atgggttcat ctaataaata cacagctgta
     3061 tcaataagat gtaaaaacaa atcataaagg acgaactctg tcgtttcttt tgccgctatt
     3121 cgattttttt gcaggtggat caaccgctta tctgggatac gtttcaattc ttctaccata
     3181 ggagcaaagc gccggttgaa accgatcatc aaaattattt ggttcttatc agctaattct
     3241 tgcaattcac gaacttcatt tagatggaca ctcagtggtt tgtcaatata aacatggata
     3301 ccattttcta gacatttttt ggctaattca taatgtactt ttgttgctgc gtggatcata
     3361 catgcttcga ttttttcttc taaaagctca tcgatcgttt catatagatg ttggaacccg
     3421 tactcttttt gaattctttc ttttgtttct ttatttcttg ttgcaaaata aaaatcaagt
     3481 gttccttgtt ttttcgcata agttggtaaa tatgcttttt ctgcgatatt ccctacaccg
     3541 attacaccaa tcttcatcta ctttcctcct ggatcattta gttactttca tcttacacca
     3601 tttttttcta aaatgaggta caatagtatc gcaaaggtaa ggaaattaat tcgaaggagt
     3661 gtgcttttat tgaaatttat ctcttggaac gtaaacgggc ttcgtgccat cgtaaataaa
     3721 aattttttag aagtttttca tgagttagac gccgattttt tctgtctgca ggaaacgaaa
     3781 ctccaagcag gacaaattga tttagatctt ccaggttatt atcaatattg gaattacgcg
     3841 gaacgaaaag gctattccgg cacagcgatt tttgctaaaa aaccagcatt gaatgccact
     3901 tacggaatgg gtatcgatat ccatgataca gaaggccgtc tgattacttt ggaatattcc
     3961 gatttctttc ttgtaacctg ttacacgcca aattctcaaa gcgaattaaa acgtttagat
     4021 taccgattag aatgggaaga agccttttat aattatttgg aaaatctaaa aaaacaaaaa
     4081 ccagtcatcg tgtgtgggga tctaaatgtc gctcatcaaa agattgattt gaaaaactgg
     4141 aaaacgaatc aaaaaaatgc tggattcact ccagaagaaa gagcggctct ttctcgactg
     4201 ttggataacg ggtttatcga tacattccgt tatttttatc caacccagga aggtgtctac
     4261 tcttggtgga actaccgatt caattcaagg aaaaacaatg ctggttggcg gatcgattac
     4321 tttttgacga gcaaagattt agagccagga ctagctgatg caaagatcca tacggacatt
     4381 atgggcagtg atcattgtcc agtagaatta gatttgaaat aaagaggttg tgaaaaagtc
     4441 gtttagtttc gagtattaag aaaaattttg tgaaaatggc tttccacatt ttgcataaat
     4501 ttggcataat accgagaaac tggcttttga acactgttta tcgagagatt gtgaaaaagc
     4561 tgtcctgcat caagtaataa gaacagccaa acaaaaatag ttcctcatat ttttcgtttg
     4621 gttgggctta ttaccgcaga tacaagcttt tgaacaccgt ttattaagag gttgtgaaaa
     4681 agctgcactg catcaagtaa taagaacagc caaacaaaaa tagttcctca tatttttcgt
     4741 ttggttgggc ttattaccgc agatgcaagc ttttgaacac cgtttattaa gaggttgtga
     4801 aaaagctgca ctgcatcaag taataagaac agccaaacaa aaatagttcc tcatattttt
     4861 cgtttggttg gacttattac cgcagatgca agcttttgaa caccgtttat tgagaagttg
     4921 tgaaatcagc gtcctgacct gagtattaga gaaacaaatg gcaaaatagt tcctcatatt
     4981 ttgctatttt ttgagctaat accgagggtc agctgattga acaccgttta gtggagaagt
     5041 tgtgaaaaag tcgcctactt tcgagtcgta agaaattttt ttgaaaatgg ttcctcacaa
     5101 ctttgcataa atttgactta ataccgagaa gtcatgatcc ccatctaact ccaaatcaca
     5161 aggaatgagt tcaaaaacta gcttttctca acaaatatta cataaaaaat tctatgtttt
     5221 ttgttaaatt tatttgcttt tttcaaaagt atgttacagt aatttcccaa ggaggtttta
     5281 cagaaatgaa ttttgttgcc atggattttg aaactgccaa tcatcagcct tatagtgctt
     5341 gttctcttgc gctagtcatg gtaaaaaata gccagatcgt tgatgaattc tatacattga
     5401 tacagcctga gactcctttt ttctggcgaa atgtccaaat tcacggaatc catcaagaag
     5461 acgtacgcaa tgcgccgaaa ttcccagaag tctggcaaac tatccaacca tattttcaaa
     5521 aaaatcgttt agtcgttgca cataatgctg cttttgatac aaaggtctta gcgggatgct
     5581 tagattatta tcatcttgaa cagcctaatt atctttcttt atgtacagtg aaaagtagcc
     5641 gtcgcctgtt tcctgaattt ccaaatcatc gattgaacac tgtgtgcgaa aatttgaaca
     5701 tccaattaga tcatcaccat gatgcattgg aagatagtcg tgcatgtgcg gaaatcctgc
     5761 tttatcaaga aaaaaaattc gggacagatc cattgaaaaa acttgtgact ataaagtaaa
     5821 agaatactta tgaaacacac aaagaggtcg tgagcctgcc gtttcactcc gagtcacaag
     5881 gagcaatttc aaaaaatagc ttctcatgtt tattaaatat tgtgactggt gaccgaggag
     5941 ctggcaggag aacaccgttt attggagagg ttgtgaaaaa gctgttctgc atcaagtaat
     6001 aagaacagcc aaacaaaaat agcttctcat atttttcatt tggttggact tattaccgca
     6061 gatgcaagct tttggacacc gtttacc
//
LOCUS       DADVXU010000128         6029 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.133, whole genome shotgun sequence.
ACCESSION   DADVXU010000128 DADVXU010000000
VERSION     DADVXU010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.133"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(68..370)
                     /locus_tag="KB953_002585"
     CDS             complement(68..370)
                     /locus_tag="KB953_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867511.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(590..956)
                     /gene="ssrA"
                     /locus_tag="KB953_002586"
     tmRNA           complement(590..956)
                     /gene="ssrA"
                     /locus_tag="KB953_002586"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1127..1900)
                     /locus_tag="KB953_002587"
     CDS             complement(1127..1900)
                     /locus_tag="KB953_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBB6867512.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2044..2394
                     /locus_tag="KB953_002588"
     CDS             2044..2394
                     /locus_tag="KB953_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBB6867513.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2375..3091
                     /locus_tag="KB953_002589"
     CDS             2375..3091
                     /locus_tag="KB953_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBB6867514.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3091..4539
                     /locus_tag="KB953_002590"
     CDS             3091..4539
                     /locus_tag="KB953_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBB6867515.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4610..5119)
                     /locus_tag="KB953_002591"
     CDS             complement(4610..5119)
                     /locus_tag="KB953_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867516.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5109..5834)
                     /locus_tag="KB953_002592"
     CDS             complement(5109..5834)
                     /locus_tag="KB953_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867517.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         2052 a         1050 c         1170 g         1757 t
ORIGIN      
        1 aaaaatatcc cttttataag tatggtgata aaatccgttc cgggctacct tttttaggta
       61 gcctacttta atctttatac ctttctggat caacgaaagt atacttcaaa tagtcataac
      121 gccgatgatc gctccgtgcg tctggcacat cagtcacgat atcaaacaaa aaatacacat
      181 ctttcttcat tctggttttt gttgccggaa ttttaaagta gttcttatta gagtaataga
      241 gattgattaa taagctatct tcgattgcta aaaagaaaac ttctgaatcc cacaccttat
      301 aaaaatcttt gacaaatcta ttcgaaggat caaatttaaa ccataattgt gtttttcctt
      361 ccatcaacat aatgttcacc tcaaaaagag tatacgaact aatgttcttt ttgtaaagta
      421 ggaatatcat tgataatatg gatggaacat atatattaaa aagtgttctt tttgaatgct
      481 attttgaatg ccattttcat tcatttaacg aaaatctgcg aaaataaaaa atgctcaaac
      541 ccttataaaa taaggattta agcatctagc gaaatctgct gaaatctcac aatggagacg
      601 gcgggagtcg aacccgcgtc caaacacatc gccacttaaa aatctacgtt catagtcaac
      661 tcatttaagg attcacttta cagcttgccg agtgacaggc attctgtatc gctagtctga
      721 tgttctcttt ttcaacttac agacggaaag ttgaaacgta tcccactaaa gttaggaccc
      781 ttactcgagc acatgggcga tgccgagagg atctacgcta actgttttta ggcagctaaa
      841 gcgtaagagt tgttttcgtt tttagcagtt atatttaact gtaacgtttt tacgtagacg
      901 taacctacga aacgcaattc aagctcgact gtgcctgtcg aatccgtaac gtccccgtta
      961 gctaacaagg gttacgtgta tttttcatac aaaattagta tagcatatat tgggataaat
     1021 tcaataaaaa agcgaatcat atcccattat ttatataata actgcccctt tcttaagaaa
     1081 aaataagcga ttttcttaag aaaagaggta gcttcaaaaa aagatcttaa atcagatcaa
     1141 aatacttcaa tgcgtggaca attccgcctt ctgtattttt ctttgtgata aaggtagaaa
     1201 gttcttttaa tccgtcatag gcgttgccca tagcgatttt gttgtcgcaa gcttccaaca
     1261 aggcaaaatc attcggacca tcgccaaatc caaaagtagg aactttttcc agctgaagtt
     1321 ctttttgcag acgtttgaca cctgcccctt ttgagattcc tttcttcact gtatcgatcg
     1381 agtaaggacc attccgataa aaagttagct caggaaaatg ttcgtgatag tattcgtcac
     1441 ctgattgaga taagatcagc aacatattta ctgtgttttc tttaaagcct aaaggatcaa
     1501 tttccggaac atctgaatga atgaagtcgt aagcattgat cattgtttgg ctatgaccgg
     1561 tacaccagat tttctgatcg ttatagtaag aaagttcatg accgttcaac tgtacatgat
     1621 catacatttt ttgacattct tctgaagaaa atgtttgaga atagatttct tcgccgtcta
     1681 ctcgaatgaa agaaccattc atggtgaccg cagaagtaat gcccgcatct tccatgatag
     1741 agtggatttc tgcttctgta cgtcctgtag cgatgacggg gagaacattg ttttctttta
     1801 atgcgttcat agcttttgcc acttcgggtg tgatttttga tttcgcatct agtaatgttc
     1861 catcaagatc aaagaaagtg attgcacgat aatttgacac aaataaaact ccttttttat
     1921 tggaataagt ttatcttaac aaagaatgaa caaaagacaa acctttcgtg aagaattcct
     1981 gaacatattg gttcagtcaa caatttagag tatactataa gaagagaatt taggagggaa
     2041 agaatgattt ttttcagtat tggtatctta ttcttaataa ttggcagtct ttttcggatt
     2101 ctgccctcaa aaggaaatct gcctttttat gggtatcatt cgcctcttgc cgcaaaaaca
     2161 gacgctcatt ggcggctggc tcaaaagaca agcggaaact ggttttttct tatgggctta
     2221 ctgatggcat tgatcggcta ttatttaaag acatctgggc atacgaatta ttttctgatt
     2281 gaaatgcttc tgctagtatt tccaattatg cctattttta ttatgacaga aaagaaatta
     2341 cagaaatatg acctggaaac tggagggaac gataatgaat attttaatga ttgaagataa
     2401 tcaatctgtt tctgaaatga tgcagatgtt tttcttaaat gaaggttggg aagcaacttt
     2461 caagtatgat ggaaaagaag ggctcgatgc ctttttagaa aacccacaac attgggatat
     2521 gattacacta gacctgaatt tgcccacact ggacggtatg ctagtaggaa gagagatccg
     2581 caaagtttca agtactgtgc cgattatcat gctgacagct agagattctg aaagcgatca
     2641 ggtgatcggc ttagagatgg gggcagatga ttatgtcaca aaacctttca gtcctctgac
     2701 gcttattgct cgaatgaaag ctttgcatcg ccgtagtgag ttggcagaag caagagaaga
     2761 agcagtagtt tcagatgatc attttgatat cgagactagt catttcaaga tgaatacgaa
     2821 aacacgagaa gcgtatctgg acaaccggcc aattgaagga ctgacgccta aagagttcga
     2881 tctattgttt actttagcaa aaaaaccacg tcaagtattc tctcgggagc agttgcttga
     2941 attagtctgg gattatcagt actttggtga tgaacggaca gtggacgccc atatcaagaa
     3001 attacgacaa aaaatcgaaa aggtcggacc acaagtcatc caaactgttt ggggagtcgg
     3061 ctacaaattc gatgattctg gtgttgccta atgcgttatt tgtatcagca actcttagct
     3121 ttctgcggaa tggtcgcgtt gatcttattg atcgttggag tttcttttac acaactgaca
     3181 aaacaaacga ttgaagaaaa caattaccag caactgtttg gatatgctga atcagttgaa
     3241 aagacaacgc aaacctatgc cgatacgctc ccgcagttta gtcaagatca ggcgtttcaa
     3301 aatgcattgc tgctgacaga gcaagtactg acagaacaaa atgtgaactt tatatttatc
     3361 gataaaaacg aacgtgtcat ttatccgact aatacagctt tactgaattt taccttaaca
     3421 gctgatcaat ggaaaaatct gaggaatgga cgtcaagaaa aattcacatc gaataaaaat
     3481 attttaggac aagcacaggc gacctcttac gcacttgtgc catttaattt gaatcatgaa
     3541 ttctatggag cacttgtagt tagtcaacct gcgcgaaata tcgataacag cgttcgtcca
     3601 attacactga acctgttcaa aggatttatt ttttccagta tcgttgcagt tattgccagc
     3661 tatgcttttg cagcattcca agttaagcgt atcaatcggt taaggaatgc gacgaaagaa
     3721 gtgacgaatg gtaattttga tgtacagctc cccgtacatg acaaagatga atttgatgag
     3781 ttagcagatg atttcaataa aatgacgaat tcattgaaag aatctcaagc agaaattgaa
     3841 gaacaagaaa atcgtcgaag acaatttatg gcagatgctt ctcacgagat gagaacacct
     3901 cttacgacaa tcaacggact tctggaaggg ttagaataca atgcaatccc tgaaaatcag
     3961 cgagaaaacg cgattaaatt gatgaaaaat gaaacagagc gtctgatccg tttggtgaat
     4021 gaaaacttgg attatgaaaa aatccgtacg aatcaaatca gtatggtcat taaaaaattc
     4081 aatggaacag aaacattgaa aaatattgtt gcacagttag aagctaaagc agaagcagca
     4141 ggagatacat tgacattgaa agccgttgat gatattgatg tttacgctga ttatgaccga
     4201 tttgtccaaa tcatggtcaa tattattcaa aacgcgatac agtttactga aaacggccaa
     4261 atcacgatta ctttagaaaa aggctatctt gaaacgatca tcacgattga agatacggga
     4321 attggaatgt ctgaacaaca aatgaaaagt atctgggatc gttattataa agtagatcct
     4381 tcaagaaaaa atacaaaata tggtgaatca ggattaggtt taccaatcgt gcagcaactt
     4441 gtacgcttgc ataaaggaaa attagaagta gaaagcgaac taggaaaagg aacaaaattc
     4501 gctgttcgtt tgcctgacgt agaaatcaaa aaagagtaaa aaacaagcgg tataaaagat
     4561 catgaatcga tcttctatac cgcttgtttt ttactataaa atgattgatt tagcccgcgg
     4621 gctctaaacg atcaatataa ggactttgag caatagtagc atcatgagta aagagtaaga
     4681 taccttgatt ttcttcatga gctaaataag aaagcaagtg gagtagctct tggatctcca
     4741 atacactaag ttcatcaaat gtatcttcaa taacaatatt gtttcgcttg gaaagcaagg
     4801 catgaagtaa ttgcagcttt actttttcaa aagacgtcaa ctcagctgcc gcacaattaa
     4861 gcaaggaagg agagatatca agtttttctt gataggccaa acgatcttgt ttgatttctt
     4921 tttccggtac atccaacagg acatggtctt taagattcag ataaggaaac atcggccaat
     4981 ttttttggat gatcgcaaaa ttatccagtt ttgcaacaag aacctctttt ttcaagtgct
     5041 ccaatatttt atgctgtttt tcgagaggac acaaaataac ggtcaagtgt tcagcatgaa
     5101 tcaaatattc aggtttcatt acgtccaggc ctcctacttt gcaaatgatg gataaaaaaa
     5161 gtgaaaggga atagactaag aatgagacag catccactga gcccaataag agtttgggca
     5221 aatcccttaa gtatatctgt gaaaaaaaga tcattttgga aatcgatggt aaaaaaagtc
     5281 tgttgatcca tcggaattgt aagtccgctt accgaagtca agatatcacg gatatctggc
     5341 agttcagtag tctttaagac aagaaaattg atcctttgaa gaagcgcttc ataaactgac
     5401 tggaagctta cgatccacaa taaaaccaag atacaacaaa gaaagagagg aaagaacagc
     5461 tgccagaaaa taaaaaggaa taatttggtt ttagaaagac cagtcaggtg ccaattgatc
     5521 aattcctgct tgttttgcct gactgaatag taaaagaaaa agataaaaag gaaggcaaat
     5581 agaaaaaata aactgaatat gccttttata taaaatcggc tagctaattc taaagccgga
     5641 agaagctctg tagatgcaga agcgccaata aatgaaagcc gatcttggac ttgattggct
     5701 aaagccgatt gcaaatggta aagagtcagg catgctatca ataaaaaagc agaaaagctt
     5761 gaaaataaac aatagagtat tgtctgcttt ttgtaataaa ttaatcgagc agtagcatag
     5821 cggattattt tcatactttt cacctctata aaaagtatag aaaatgaata tgaataaagt
     5881 atgaaattcc tttagtgaaa tcacgaatta aaaccagaaa atattttgta aaaagcaaga
     5941 ggttgtgaag aagctgccct attctattac cgtagatgca agcttttgaa cactgtttat
     6001 tcgaaaagag gagcagcgac aataatttt
//
LOCUS       DADVXU010000129         5989 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.134, whole genome shotgun sequence.
ACCESSION   DADVXU010000129 DADVXU010000000
VERSION     DADVXU010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5989
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.134"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..450)
                     /locus_tag="KB953_002593"
     CDS             complement(<1..450)
                     /locus_tag="KB953_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBB6867518.1"
                     /translation="MKYKEMFVPSLIQLAVEVKNKEEAFLIAAQQLEKMGYVNENYLN
                     GLIAREREFPTGLITKYLNIALPHSECEYVEKPFVFVMQLKNPIYFYQMGDNQEMTVE
                     NLFFLGIKEPKEQVGLLQAFMNLFMDETFVENYKKQETPQNIYELITN"
     gene            674..1465
                     /locus_tag="KB953_002594"
     CDS             674..1465
                     /locus_tag="KB953_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBB6867519.1"
                     /translation="MLKKERQEQIITLLEKDFYLTNSEIANKLGVSEMTIRRDVTELA
                     KKNKVKKLYGGVERKEPYNRELSTQEKIDTNVAEKKYIGQVMNSMIPDNSTIYVGAGT
                     TILYALPFIQKKNLFVITNSLIAFNYLKNHTSYRILLTGGEFSPITEEFIGEVAERSF
                     ESLNVDISFAATNGIFDNNVTTAQFIEGNIQNAALNAAKIKCIVADYSKLNKSDVYTF
                     RKLTDFDYLITDNHLSEQDFNHYSKYLTILKEPKNDTDNHDEPIH"
     gene            1431..2384
                     /locus_tag="KB953_002595"
     CDS             1431..2384
                     /locus_tag="KB953_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HBB6867520.1"
                     /translation="MILTITMNPSIDSIYFTKHFELGTMTRFDSPVRYVGGKGINCGR
                     ASALLGSSVILTGFLGGYFGSMIQDMLKNENLFDLHFLPIAEESRGAVTIMHDDETQT
                     ELVEAGPEIADTTIKKLMNDIKLISKNNYLSVITINGSVNNPDPFLYAKLLQYIKEQI
                     DPSTYILMDISGQHLREVLASKYKPMFIKPNIHEFSDIIHQKITQKSDIKHHLKTNPL
                     FKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATVGGFAHSLEQNYPL
                     KECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK"
     gene            complement(2782..3756)
                     /locus_tag="KB953_002596"
     CDS             complement(2782..3756)
                     /locus_tag="KB953_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBB6867521.1"
                     /translation="MNKELNITQEWDKVFPKSNQVNHRKVSFPNRFGITLVADLYEPK
                     EVSEKKLPAIAVSGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGVPRYVAS
                     PDINTEDFQAAVDFLSVQKNVNPERIGIIGICGWGGMALNAAAIDTRVKATVVSTMYD
                     MTRVTANGYFDVMDENARYEMREELNAQRIIDFKQGDYARAGGVVDPLPEDAPQFVKD
                     YHAYYKTDRGYHVRSLNSNDGWNRTSGLSLMNAKLLHYIPEIRHAVLVIHGEKAHSRY
                     FGEDAFKELKGDNKELMIIPGASHTDLYDQTDIIPFEKMATFFEENLV"
     gene            3871..4305
                     /locus_tag="KB953_002597"
     CDS             3871..4305
                     /locus_tag="KB953_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBB6867522.1"
                     /translation="MYSIGQVSEMFGLPVSTLRYYDKEGLFPDIQRVSGIRKFSENEL
                     EALRVIDCLKKSGLEIKDIKQFMEWSKEGSKTFEVRKELFEKQKEVVEKEIAKLERVL
                     AMLNYKSWYYEEAIKAGNEEAVLTMIPDDLPQHVKEAYVHSH"
     gene            complement(4430..5452)
                     /locus_tag="KB953_002598"
     CDS             complement(4430..5452)
                     /locus_tag="KB953_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HBB6867523.1"
                     /translation="MKKIGFLSFGHWMEQGSLVKSAQDAYLQSIDLAVEAEKIGVDGA
                     FFRVHHFAPQIGSPFPLLAAIGAKTSKIEIGTGLIDMRYENPYYMAENAGLTDIITQG
                     RVQLGVGRGSPEQVLDGWKYFGYDYSEEEIKEVTRERTLEFLELLEGKPFEEPNPQPM
                     FPQGSGKLRIEPYSEGLRQRIWWGAGSQQTAIWAAQHGMNLQSSTLVNYESHEPFHIQ
                     QANQLRAYKEAWKQAGHDFEPRTLVTRSIQPITTDLDRRLFGQDGSPQQDAVGYLAYH
                     RNPTIFGKTFAGEPDQLIKELAEDEAIKEADTVLVTIPNTLGVEYNMHLLETIVKDIA
                     PELGWR"
     gene            5844..>5989
                     /locus_tag="KB953_002599"
     CDS             5844..>5989
                     /locus_tag="KB953_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HBB6867524.1"
                     /translation="MKKIYFLCTGNSCRSQIAEGYARKYLSPSMFEIRSAGIETHGLN
                     PRAI"
BASE COUNT         1885 a         1129 c         1031 g         1944 t
ORIGIN      
        1 atttgtaata agttcgtata tgttttgcgg tgtttcttgt ttcttgtaat tttccacgaa
       61 tgtttcatcc atgaataaat tcataaacgc ttgaagtaat cctacttgtt cttttggttc
      121 tttgattcct aaaaaaaata gattttctac agtcatttcc tgattatccc ccatttgata
      181 aaaataaatt gggttcttca gctgcataac aaaaacaaat ggcttttcga catattcaca
      241 ttccgaatgt ggcagcgcga tatttaaata cttagtaatc aatccagtag gaaattctct
      301 ttctcttgca atcagtccat tcaaataatt ctcattgacg taccccattt tttctaattg
      361 ttgtgcagcg attaaaaaag cttcttcttt attttttact tctaccgcta attgaatcaa
      421 actaggaacg aacatctctt tatatttcat tccaacctcc tcctttttga ttaagtttgt
      481 taatttattg ttctactaaa ttcatctttg ttttacaaga acattatatt tctgttagtt
      541 tgttttgtca acgctttcaa aagaaattta ttttgttaaa tttgatctac ttgaaaataa
      601 aatcgtttat ctatataatt aagaagaaca aagtcgaaca aaaattgact tttatgttaa
      661 aggagtaatt catatgctga aaaaagaaag acaagaacaa atcattacat tattagagaa
      721 agatttttat ttaactaatt cagaaatcgc taacaaatta ggcgtatcag aaatgaccat
      781 acgccgagat gtcactgaat tagcaaaaaa aaataaagta aaaaagctat atggtggcgt
      841 tgaacgaaag gaaccttata atcgagaact atcgactcaa gaaaagattg atacgaatgt
      901 agccgaaaaa aaatatattg gtcaagtcat gaacagtatg attcccgata attctactat
      961 ttatgtcggt gctggaacaa cgatcctata tgcacttcct ttcattcaaa agaaaaatct
     1021 atttgtaatt acaaacagtt tgattgcttt caactattta aagaatcata caagttatcg
     1081 gattttactt actggtggcg aattttctcc tattacagaa gagttcattg gggaagttgc
     1141 agaaagatct tttgaaagtt tgaatgtcga tatctctttc gctgcaacaa acggcatatt
     1201 tgataacaat gtgacaaccg ctcaatttat tgagggaaat attcagaacg cagcgctcaa
     1261 tgcagcaaaa atcaaatgca ttgttgcaga ttattcaaaa ttaaataaat cagacgtcta
     1321 tacttttaga aaattaaccg attttgatta tctaataacc gataatcatt tatcagaaca
     1381 ggatttcaat cattattcaa aatacttaac aatattaaag gagcccaaaa atgatactga
     1441 caatcacgat gaacccatcc attgattcca tttattttac aaaacacttt gaattaggta
     1501 cgatgacacg ctttgattct ccggtacgct atgtaggcgg caagggaatc aattgcggac
     1561 gagcttctgc tctactaggt tcatcagtta ttttaacagg gtttttgggc ggttattttg
     1621 gcagtatgat tcaagatatg ctgaaaaatg aaaatttgtt tgatttgcat tttttaccta
     1681 ttgcagaaga atcaagaggt gccgtcacga tcatgcatga tgatgaaacg cagacagagc
     1741 tagtggaagc tggtcctgaa atagcagata ctactatcaa aaaattaatg aatgatatca
     1801 aactaatcag taaaaataat tacctttcag tcattacaat aaatggttct gttaacaatc
     1861 cagatccctt tttgtatgct aaattattac aatatatcaa agaacaaata gatccttcca
     1921 cctatatatt aatggatata tctggacaac atctccgtga agtcttagca agtaagtata
     1981 aaccgatgtt catcaaacct aatatccatg agttttcaga tatcatccat caaaaaatta
     2041 ctcaaaaaag cgacataaaa caccatctaa aaacaaatcc actatttaaa gatatcgatg
     2101 ttatcatgat ttcttgcggc tcgtatggtg cagtagtcaa agtaaaagaa gaattttgga
     2161 atgttgagat ccctaagatt cctatcatta atacaacagg ctctggggat gccactgttg
     2221 gaggttttgc tcattcatta gaacagaatt atcctttaaa agaatgtgta aggtacgcaa
     2281 tggcttgcgg catgtcgaat gcacagcaat cagaagtcgg gaaagttatt ccagctgatg
     2341 taaaaaattt agcaaaaagc attaaaatca cagaaatgaa ataaatagta caaagtaaat
     2401 aattgtttat atgaaatgag acacaaagag actgtgacaa aaaacgtgtg taccgacccc
     2461 caaaagttag attttttggt ctaacttttg ggagtcgata cattgtcaca gtcctttttt
     2521 cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
     2581 gagaagctat tttttgaaat tgctccttgt gactcagagt taaacggcag gctcacgacc
     2641 tcttggtata aacggtgttc aaaagcttgc atctacgcta atgagtccaa ccaagtcaaa
     2701 aatgaggagc ttttttggct gtccttatta cttgatgcag ggcagttttt tcataatctc
     2761 ttttagatta aatccttttg gttacacaag gttctcttca aagaatgttg ccattttttc
     2821 aaatggaatg atgtctgtct ggtcgtatag atctgtatgg gatgctcctg gaatgatcat
     2881 gagttccttg ttgtcacctt ttagttcttt gaaagcgtcc tccccaaagt aacgtgaatg
     2941 agctttttcc ccgtgaatga ctaagactgc atgacggatt tctggaatat aatgcaatag
     3001 ttttgcattc ataagtgaca accctgatgt cctattccaa ccatcattgg agtttaaaga
     3061 acggacatga tatccgcggt ctgttttata atacgcatga tagtctttga caaattgagg
     3121 agcatcttca ggcaacggat caaccacacc tccagcacgt gcgtagtccc cttgtttgaa
     3181 atcaatgatg cgttgtgcat ttaattcttc acgcatttca tatcgggcat tttcatccat
     3241 aacatcgaag tagccatttg ctgttactcg agtcatgtca tacattgtgg aaacaactgt
     3301 tgctttgacc cgcgtatcga tagctgctgc attcaatgcc atacctcccc atccacaaat
     3361 accaatgatt ccgattctct cagggtttac atttttttgt acagataaaa agtctactgc
     3421 cgcttggaaa tcttccgtat tgatatcagg cgaagcaaca tatcgaggta ctcctccgct
     3481 ttctcctgta aacgatggat caaacgcaat cgttaaaaag cctctttcag ccatcgtctg
     3541 cgcatataga ccagacgatt gttccttcac tgcaccaaat ggcccgctca cagcgattgc
     3601 cggcaatttc ttttcactta cttcttttgg ttcatataaa tcagcgacta atgtgatacc
     3661 aaaacgattc gggaagctca cctttcgatg attgacttga ttgctttttg gaaatacttt
     3721 gtcccattct tgtgtgatgt ttaattcttt attcatattc tagactctcc tttgatcaaa
     3781 attgacattt gtttttgaat gtattattat gataaaactt aaacttgact ttaggtcaag
     3841 gtaaaaattt tattttagga ggattttttt atgtattcca ttggacaagt gtctgaaatg
     3901 tttggattgc ctgtttctac tttacgttat tacgacaaag agggattatt tcctgatatc
     3961 cagcgtgttt ctggtattag aaaattttca gagaatgagt tagaggcttt aagagtcatt
     4021 gactgtttga aaaaatctgg tttagaaata aaagacatta agcaattcat ggaatggagc
     4081 aaagaaggca gtaagacatt tgaggttcgt aaagaactat ttgaaaaaca aaaagaagta
     4141 gtagaaaaag aaatagcaaa attagaaaga gtactcgcta tgctaaacta caaaagctgg
     4201 tactatgaag aagcaataaa agccggcaat gaagaagccg tattgacaat gattcctgat
     4261 gatttacccc aacatgtaaa agaagcttat gtacattctc attgaacaaa cggataactc
     4321 actctccttc tctatgaact tcatgaaaac atgaataaac acctctaact gattgttaga
     4381 agtgtttatt ttttcactca agtatcctgc acttacaaaa atatcctttt taccgccatc
     4441 ctaattctgg agcgatatct tttacgattg tttcaagcaa gtgcatgtta tattctaccc
     4501 ccaaagtatt aggaatcgtg acaagtactg tgtctgcttc tttgattgct tcatcttcag
     4561 ctagctcctt gattaattga tcaggttcac cagcaaaagt ttttccaaaa atcgttgggt
     4621 ttcggtgata agcaagatag ccgacagcat cttgttgcgg actaccgtct tgtccaaata
     4681 agcgtcgatc caaatcagtg gtgattggct gaatcgagcg agtaactaat gttcttggtt
     4741 caaaatcatg acctgcttgc ttccacgctt ctttatacgc acgtaattgg tttgcttgtt
     4801 ggatatggaa tggctcgtgg gattcgtaat tgactaatgt agatgattgc agattcattc
     4861 catgttgtgc tgcccagatt gccgtttgct gcgaaccagc tccccaccag attctttgtc
     4921 gtaatccttc tgaataaggt tctatccgta attttcctga tccttgtggg aacattggtt
     4981 gaggatttgg ttcttcaaat ggttttcctt caagtaattc caagaattca agggtgcgtt
     5041 cccgtgttac ctctttgatt tcttcttcac tgtaatcata accaaagtat ttccagccat
     5101 ctagaacttg ttcaggtgaa ccacgcccca ctcctagttg cactcttcct tgcgtgatga
     5161 tatctgtcaa tccggcattt tctgccatat aataagggtt ttcatatcgc atatcaataa
     5221 gtcctgtacc gatttcaatc ttacttgtct ttgcgccgat tgccgctagc aaaggaaacg
     5281 gcgacccaat ctgaggggca aaatgatgta cgcggaaaaa cgctccgtcc actccgattt
     5341 tttctgcttc caccgctaaa tcaatcgatt gcaaataagc atcttgagct gatttcacaa
     5401 gcgatccttg ctccatccaa tgtccaaagc ttaaaaagcc gatttttttc aaggtaatga
     5461 cctcctttaa acctacaaaa atggaaggtt ttttattttt aatatgaact gattctacta
     5521 ctttttttct taaaagacga ataattgttc tcgcctcaca atcaagaaat gtatcggctt
     5581 actacacccc tactttcttc atcatctgcc gtgtcttaat ctcttttaac agtctgttac
     5641 atcgttgatt tttgactcaa aatgattgtt tctaacatgt atttatggta aaaaagacaa
     5701 gattagttct ttgtacacat cgatttatac gctgaacaaa aagaaatata taaactaaga
     5761 aacccattgt tttttcgctc catcacatag agtttacttt agataaacag tgacatttac
     5821 taatccataa cggaggaaat caaatgaaaa aaatctattt cttatgtacg ggcaattcat
     5881 gccgcagtca aatagcagaa ggctacgcgc gtaaatactt gtctccttca atgtttgaga
     5941 taagaagtgc ggggatcgag acacatggct tgaatccaag agctataaa
//
LOCUS       DADVXU010000130         5832 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.135, whole genome shotgun sequence.
ACCESSION   DADVXU010000130 DADVXU010000000
VERSION     DADVXU010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.135"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(58..516)
                     /locus_tag="KB953_002600"
     CDS             complement(58..516)
                     /locus_tag="KB953_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBB6867525.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(529..1860)
                     /locus_tag="KB953_002601"
     CDS             complement(529..1860)
                     /locus_tag="KB953_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HBB6867526.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1871..3313)
                     /locus_tag="KB953_002602"
     CDS             complement(1871..3313)
                     /locus_tag="KB953_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBB6867527.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3300..4064)
                     /locus_tag="KB953_002603"
     CDS             complement(3300..4064)
                     /locus_tag="KB953_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HBB6867528.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4168..5043)
                     /locus_tag="KB953_002604"
     CDS             complement(4168..5043)
                     /locus_tag="KB953_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBB6867529.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5298..5382)
                     /locus_tag="KB953_002605"
     tRNA            complement(5298..5382)
                     /locus_tag="KB953_002605"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5346..5348),aa:Leu,seq:caa)
     gene            complement(5400..5471)
                     /locus_tag="KB953_002606"
     tRNA            complement(5400..5471)
                     /locus_tag="KB953_002606"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5437..5439),aa:Gln,seq:ttg)
     gene            complement(5481..5553)
                     /locus_tag="KB953_002607"
     tRNA            complement(5481..5553)
                     /locus_tag="KB953_002607"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5518..5520),aa:His,seq:gtg)
     gene            complement(5615..5699)
                     /locus_tag="KB953_002608"
     tRNA            complement(5615..5699)
                     /locus_tag="KB953_002608"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5663..5665),aa:Leu,seq:caa)
     gene            complement(5720..5790)
                     /locus_tag="KB953_002609"
     tRNA            complement(5720..5790)
                     /locus_tag="KB953_002609"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5756..5758),aa:Cys,seq:gca)
BASE COUNT         1771 a         1247 c          892 g         1922 t
ORIGIN      
        1 aaaaattttg taaattcacc cataaatatc accctattta acttttttca tcgtacacta
       61 aaattcaaaa caactatctt taatctttac ttcttggttt tgttttaata aatttccttc
      121 aaaaatgcct ggagaaacta caatgatcac cgtatctttt tgtgcttgct ccaacttctt
      181 caaatctata atagctaaca gtgtcccttc ttttacctcc tggttacttt caacccgtat
      241 ctgaaacgga cttccttcga gttcaactgt atctaaaccc atatggatca gtatttttct
      301 gccagattca ctttcgatcg ttatggcatg tcgggtagga aatatattat cgattattcc
      361 tttaactggc gcatggatat atccatcatg attcgttata gcgtatccaa ctcctatcat
      421 ttttgaagaa aagacctcgt cttttactgc tgttaatgga ataatttgac cactagctac
      481 agcttttaga atgtttcttg ttttaaagaa atgcactttt tatcacctct actttaatgc
      541 tggccaatat tttttgttta ctttgatcat ttcatctagt attttttttg cgactctggc
      601 atttggtacg gtttgattca atgtaaatgc ttgtaaagct ttttgataag aaccttcaaa
      661 gtaagcatct actagtaact tctctactgc aacttgtgcc tccatcaatc ctttatgaaa
      721 atcactgatt ggttttctta gtgtaattgg ctccacacca gttgcaccaa catatgccgg
      781 tatttcaaca actgcatctg accgtagatt cggaatcgcc ccttgattag gaacgattag
      841 cataaagcga cgacgatcat cattcaacaa ggaaatagct atatcgacga tatattgtcc
      901 atgctcccca aaattaaagt tcaatacttc ttccacaata ccgcggcgta ttttatcagc
      961 catttctttg gtatttttct ctcgtccatc catgaccata ttagcacgtg tatactcttt
     1021 gtcagtatgc tcaacgacca tatcaggata taagtaatac tctaagtaat tattcgggat
     1081 attacttgga aaatttttcg tgatgactga catcatttga aaggtttttt gccaagtttt
     1141 atctcctata ttaaaatcgg cgacttgcat ttcttttgtt attagttttt caataatctc
     1201 tggcataatt tctcgttgca attctttatc ataaatcgat gtataccagc caaagtggtt
     1261 taaaccataa tacgtaacga tccaattctt acgatcataa ccgtaattca ctgcaatcgt
     1321 ttcttcaatt gaaatagtca tatcacaggc attaatcatt tttacattcg gatatttacg
     1381 acgaacagct tcagaaacga tcgactctgg atttgtataa ttcaaaatcc atgctttggg
     1441 ggcatagtct tgtatatgat cgacaatttc caataaacct ttgattgagc gcatcccata
     1501 agcaaatcct ccgagtccac acgtttcttg tcctactaat ccatgtttaa gtggaatctt
     1561 ttcgtccttt tctcgcatct ccaatcctcc aacacgaatt tgtgagaaaa caaaatcgca
     1621 tcctgtaaaa gcaagctttg gatcatccgt tgcacgaatg cgaatatttg aaaaaccttt
     1681 tcttttcagc ataaatttaa caatcaagta catatccata tttctttctt catcaatatc
     1741 atataaacgt atttcagata atggcaaccg tttttcattg ttcaaaatgg cttgaacgat
     1801 tcctggtgta tacgtactcc cgcctccagc tatagtgata atctgttttt tcatcatcat
     1861 cttttccttt ctattcttct aataatttat tgaattctgc cataacataa gtcacttcta
     1921 agccgatgat aatttgataa tttttttcag ttggtttaat gatcccgctt atccctaatt
     1981 gcttgattcg ctgttcctcg accatggtgg gatccactac ctctacccgt aggcgtgtat
     2041 tacaactata gacatccagg atattggctt ttcctccaaa cgcttgtagc atttttgttg
     2101 ccatatattc ataattttta cttgctaacc ctaactctgt ttcttctaag gttacttcat
     2161 tgccaaactc tgccttagct tcacgtccta atgtctgaat gtttctctta ttgatcaaat
     2221 aataaaaagt aacgtaatac aaggcaaaaa agaaaattcc gattggaaga atcaaaatag
     2281 cattggtagc gattcgaaaa ttgatgagat aatcaacaat acatgctccg aatgtaaaac
     2341 caattcgtat attgaataag taacagacaa tccctgctaa accggcaaaa agtgcatgga
     2401 tcacgtacaa cataggagaa agaaacatga aactaaattc aacaggctca gtgatatttg
     2461 caataaatga agttcctgca ccgcttccca tgattccttt gaccatctct ttattttctt
     2521 tataagctgc tttatagata gctagacata tagctggtat accaaacatc attactacaa
     2581 aaaaaactga taaaaacagt ccagcctgag gatctccgtt gataaagcgt gtcatctctc
     2641 ctctgaaaac ttccttgcta ggagatgtat agcttcctaa atcgtaataa atatacgtat
     2701 tcaacacatg gtgcagtcca aaggggatca gcaaacgatt caaaaacata aatatcccca
     2761 cacctaatgc tcccatatca acgagcagtt ttgcaaaatt atcaatccca atttggatga
     2821 gcggccagat aaagcccaaa aaaacagctg cgattgtttg tagaatcatc accatagtca
     2881 atgggaattt ttcgcccgca aaataagaaa aaactactgg taatttttgt tctttaaagc
     2941 gattatacgt ccatgccgct actaaaccgg aaataattcc accaaaaatc cccatattca
     3001 cttctgggtc cataatttct aagccttttt tcaaggtagc aatcgctaaa aaaccagtaa
     3061 gtgcaggtat tcctttgtcc ttatttttga caaaaccgat tgcaactcct atagcaaata
     3121 agatatccaa gtttccaaaa acggcatttc cagctgcacc aattactggg atgttcagca
     3181 tatcgtcagc tgataaacgc atgatcaatc ccgctaatgg catcgctgca actggaagta
     3241 gcatcgctct tccaagcttc tgcacatttg atttaacatt catttttatt cctcctctat
     3301 taaatatcct catatgttac taactcaatc aaaggatcat ttttcacttt ttgagcaaat
     3361 gctgaatgaa ttaaaaattc gacttggtgg gtacgaggat acacaaaact tgaaccattt
     3421 aacaattcct gatccaaata cgctgcgtga cacatgactt cgatcgtttt atcttctctc
     3481 aatatcatga gtaaacttgc caaatattct tccacactcg tattgatttg tcgttttttt
     3541 tcatcagttt ctcctacgac atcgaaacaa ggctcaaaat aatcgacatg acgaacttca
     3601 tcttttcgtt caaagttgct ccgaaccggc aaatcatatt tttttgctaa tgtaacaact
     3661 acctcctgat tttcatccaa agtatgagta tgatgatgac tgtctaaatg agttggtatg
     3721 atccccgcat gataaatttt ttgaatctga gcattccatt cctgatacag ttgatctttg
     3781 tcaatgttaa atggatgctg ataataagat aaggatctga actctccgct ttcgataaga
     3841 gtatcgacag ttttgaggag tggtttgcca cacgtcaatg ttaaatggac tcctacacct
     3901 agcctaggca tttcctttct tagtttcaca gcatgctcga aacccggcat atttgccatc
     3961 aatgtagttg atgtcaaaat tcctcgctga taagcatcca taatcccata attcacacca
     4021 tggctatagc caaaatcatc cgaattaaaa atcacttttc ccatttcatt ctccttcttt
     4081 tttctactaa aataggcgca accaaataat catagtccat ttactatgtg tcatttggtt
     4141 gcgcctatag atagttacgc ccttatttta atagattctt gctagatgaa ttgccaaata
     4201 gatgatttca ttttcttgaa tggcaagttg aaattgctgt tcgatgatat ctttaatctt
     4261 tagtgctgta caatacgctt tgctatgttt aaatgtttca gtaaaaaaac caatattttc
     4321 tactggcgtt tcttcctgat aaatcaagcg tttaaccagg aatttgatat gaaccattaa
     4381 gcgatgataa gtcagtgatt ctttatcata aacgtttgaa taggattttt ctagatggga
     4441 caaaatcgtg gtcgtcattt ctactatttt taagctcttt aaactattag gctctcctag
     4501 ctcagcatta atgatatgca tagtgatgaa tcctacttca tttgacccta actcggtttg
     4561 atatcgatga ttgattcgct cgaccacttc tactgctata tcatattcat ttggataaag
     4621 gaatctcatt tctggcaaca taattgctgt gacatctcta tcttctcttt ttcgttgaat
     4681 agcaaaataa atatgatctg tcaatgtaat atagatattt gaatctaatg gagaggagat
     4741 cctagctgtt atcagttcaa tcacttcttc cgcaaaatcc ataaattcga ttggaatctg
     4801 cgagactatc tcactgaatc gtttgatctt ttctttagaa tctaatacaa ataatttgtc
     4861 agcatcctca acatcaaatg gcttactcac tttagcagaa aagccaatcc ctttccccat
     4921 cgcaattttt tctattccat cgtcatcgat caatacagca ttatgattca gctttttcat
     4981 cacatacacc gttaattcac ctcctctata tgataaaaca aaacaacttg cagactcctt
     5041 catacatatt tttgtctcca aacaattttt aaagacaaaa aacgacctaa gaaagaaccc
     5101 tttcttaggt cgtgcctaat tgaatagtag caccctacat aatttacaaa aatagtttaa
     5161 cacataataa aagcgttatc aatatctttt ttactcgttt tatgataata cttgtatcga
     5221 attgatttgg aggtaattac ataaaaacaa aaaaaatata gcaactctct ataagaaagt
     5281 tgctatattg ataattatac cggcggccgg ggtcgaaccg gcacgtcctc aacggacact
     5341 ggattttgag tccagcgcgt ctgccaattc cgccacgccg gcaaatttaa aaataataac
     5401 tggggtagct ggattcgaac caacgcatga gggagtcaaa gtcccttgcc ttaccgcttg
     5461 gctatacccc aagaataaag ggcgaatgat gggaatcgaa cccacgaatg ccggagccac
     5521 aatccggtgc gttaaccact tcgccacatt cgccatcata aaagatgtta tttaatttct
     5581 caataaaaaa ctaagatgaa gacatcttag tcaataccgg cggccggggt cgaaccggca
     5641 cgccctcaac gggcactgga ttttgagtcc agcgcgtctg ccaattccgc cacgccggca
     5701 agttgattta ttagtactaa ggcggtaacc ggatttgaac cggtgatgaa ggttttgcag
     5761 acctctgcct taccacttgg ctataccgcc attatactat tatcaatcta taaaataaag
     5821 aataaattat ag
//
LOCUS       DADVXU010000131         5794 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.136, whole genome shotgun sequence.
ACCESSION   DADVXU010000131 DADVXU010000000
VERSION     DADVXU010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.136"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            176..1192
                     /locus_tag="KB953_002610"
                     /pseudo
     CDS             176..1192
                     /locus_tag="KB953_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1209..1358
                     /locus_tag="KB953_002611"
     CDS             1209..1358
                     /locus_tag="KB953_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867530.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPILNWKPKFNY
                     WEGNK"
     gene            1355..1801
                     /locus_tag="KB953_002612"
     CDS             1355..1801
                     /locus_tag="KB953_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867531.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1803..1940
                     /locus_tag="KB953_002613"
     CDS             1803..1940
                     /locus_tag="KB953_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBB6867532.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIISKEESA
                     S"
     gene            1977..2270
                     /locus_tag="KB953_002614"
     CDS             1977..2270
                     /locus_tag="KB953_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867533.1"
                     /translation="MINLGEWGTIAGSITAIVSLILLVIRPVTASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERSR"
     gene            2293..2490
                     /locus_tag="KB953_002615"
     CDS             2293..2490
                     /locus_tag="KB953_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HBB6867534.1"
                     /translation="MILPDKYYQVIKWTVLTVLPALSVLVATLGKAYGWNGTDMAVLT
                     INAAATFLGVITGVSAYNLKK"
     gene            2501..3520
                     /locus_tag="KB953_002616"
     CDS             2501..3520
                     /locus_tag="KB953_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HBB6867535.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGVNEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVKADGIY
                     RPDVNQAYNTLGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPQR"
     gene            3777..4424
                     /locus_tag="KB953_002617"
     CDS             3777..4424
                     /locus_tag="KB953_002617"
                     /inference="COORDINATES: protein motif:HMM:NF014505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBB6867536.1"
                     /translation="MPNKNYRVYKRGTIVYVDFGVNVGYELSGNHFAIVLNNKDNSKN
                     GVVCVVPISSKKKSNYVSVGKILEVASIKQFISQADKLKEKLRIIILLSLNRGIIDES
                     DLNSFIKDIEAKGEKLSVEEVEKRAVKHNLVCETREQVIKEIKSLSEDMVKYQKVFNC
                     YKKYTQESYVMPLNIQTISKNRIRRINKFDPSGNMKAPGNVLNEVDESLKKYFTK"
     gene            complement(4749..4895)
                     /locus_tag="KB953_002618"
                     /pseudo
     CDS             complement(4749..4895)
                     /locus_tag="KB953_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            5134..>5794
                     /locus_tag="KB953_002619"
     CDS             5134..>5794
                     /locus_tag="KB953_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBB6867537.1"
                     /translation="MNKKIVVSGLIIGGLVLAGIAGKNFADDILLWGGEDNIEQINHN
                     LESLDKALENKEQNISSLNSRLSSNNQELEQAKANVEEYKQKVLTLENDKNQLTIEKT
                     NLENQLSGKNAELQGKQNEINAKIDEINQKIAEINQLNTDWNNRLQTEQNKFNDAQNT
                     VNSLNQQINQLSNEKNTIKNQLQSTEQKLEKAQSENSNLNNYIEKLEKAKSDVEDTAN
                     RS"
BASE COUNT         2150 a          890 c         1131 g         1623 t
ORIGIN      
        1 acctaaaaat gaattgaata cgtggcagac gcttatcgga acgcgaacgg taaaatacac
       61 ctctgacgta ccaaatgaat ggtacccttt gtttagagac atccgaacac ttaaaccgac
      121 cggtaaagtt agactaagaa aaattaagat tgaagaaggc tcaacagcca ccccatatca
      181 gccaaactta cttgatgacc cttactggct aggtaaagcg cctttgggtg agaatattgc
      241 taataagtct gttacgtttc caatcaaatc tagcgcctac agcctatacc aagctaacat
      301 ggaagaagaa tttgttttag gtcaaaccta tacaattacc atgaaggcaa ccaaaccacc
      361 tatccaaacg ttcattgttt acaacgagga tagtggagat tatagatatg gtaacttaga
      421 gccagtagaa gggttagttg atacatgggg tctgactttt acaccgcaga aggttggagt
      481 taactacccc aaacggttaa ctattatcca gtatccccaa tcaacaacag gtgcatgtca
      541 aattgactgg ctcaagatcg aaaaaggcaa tactcgaacc ccgaatatta gtgagtataa
      601 atatcgtggt actggtatgc gtgattcaaa caatccaaaa gattatgttt gggatctagc
      661 accagaatat gtcgaagata atttggccac agatattaaa atttctgaaa ttactggtaa
      721 agcaaacaat tataccgatg ggaaagtatc ggagattaat tcgcagttga ctgcttcaat
      781 taatgaagta gacaccacag ctaaggatgc tcaaacaaaa gcgaatgcta atgcgactgc
      841 tatagatgaa ttagacaata agatcgatga acgcattaat gatacagcta ctaccacatt
      901 aacagttaca aacgggaata ccggatcagc aaagctttat cgtgaaggaa aaacagtttc
      961 tatatatttt gtggctttaa acggaaaaag cagtggtgga aatgattcaa cgatactaac
     1021 aattccagaa ggctatcggc caccaattag ttttgagcaa ctggttggct cgatagaccg
     1081 ttctactttg aacagtgctc agttatctat tggtgcagat ggagccatta aatggcgaag
     1141 aaactcaagt tatggatcgg attatacctt tgcaattact tacacgattt agaaaagcgt
     1201 gaatcgatat gaaggcagca tatagaccaa ttgaacctta cggattcgag caaatcattg
     1261 tgaatgatga agaacattta ccggaagaat gcacagaagt cgaaccaccg attctaaatt
     1321 ggaaaccgaa attcaattac tgggagggaa ataaatgaaa aacatttgga aatatggacg
     1381 tactggcgga gagtacgcag gaaaagtatt ggacgacatg cttgtatccg ttccttacac
     1441 agatcagcca ccgcttgaag gaattcgtgc tgatggcgaa ccgttaacaa tcgctgatca
     1501 aatgtttgat cctaaattga atcaatggat tattttagcg aacgcactag atcacaacga
     1561 tttaaacaat ctcaaagcaa tgtatgagtc gttagaaaat gagaacggcg atttaaaaca
     1621 gatcaatgcc aaactcatgc taagcgatgt agcaattaaa caggaaaata ctgcattgaa
     1681 agaaaaagcg gatagtttag cacaaatcaa ttcaaaaatg atgcttgctt cgttacaaaa
     1741 tagcaaagac atttcagaaa ttaaagagca actaaatcca gcttcaaagg gaggtgagta
     1801 gtatgtttag ttttagcgat gtgaaaatga tgtatgattg gggctgtttt actgacgatc
     1861 aagttcgact attcgttcca ctatgcatta cagacgaaga agcagataaa atcattagca
     1921 aagaagaaag cgcatcttaa gtgatgcgtt tttatttaag gtaaaggagt tgtcacatga
     1981 tcaatttagg ggaatgggga acaatcgcag gatcaattac tgcgattgtt tctttgattt
     2041 tattagtaat aagaccagtc actgcatctt tctcgaagat tactgaaact ctttcaaaag
     2101 taagtcacaa tttagatttg ctgactaaag atttagaatc gagcaaatca gatcgattga
     2161 tgattcatga agaactaaag aaacacgatg aaagattaga tacacatgca gaaaaattgg
     2221 tagaacacac acaacaaatt aaaactttat ttagagaaag gtctcggtaa aaaagaaagg
     2281 agttaagaag aaatgatttt acccgataag tattatcaag tcattaaatg gacagtttta
     2341 acagtattgc cagctttatc tgtattagta gccacattag gcaaagcata tggatggaat
     2401 ggaacagata tggcagttct gactatcaat gcagcagcaa catttttagg tgttatcact
     2461 ggtgtgtcgg cttataattt gaaaaaatag gaggaaacaa atgaaaaaga aaattactat
     2521 tactgcgatg agcctattaa cggctctttt tttattgccg attaatgggt ttgcctatac
     2581 gattaacaat gaatttaatt taggtgtaaa cgaaggtagt tctcaagtag caaataatca
     2641 gtacatttta ctgcatgaaa cggctaatga aacagcaaca ggacgcaatg aagcgcagta
     2701 tatgcaacgt tcatggacta gcgcttatac tgcttatatt gtgggagacg gcggaattgt
     2761 ttatcaagtc ggtcaacctg gttatgtaca gtacggtgct ggttcgtatg ctaatgcaaa
     2821 tagtcctgtg cagattgagt tacaacacac acatgataaa gcaacgtttg agaaaaacta
     2881 caaggcatac gttgaattag ctagagattc agcaatgaaa tatggtattc cacttacact
     2941 agacacaccg tataatcaac cagggatcaa atcgcattta tgggtaacac aaaatatttg
     3001 gggcgatcat acagatcctt acggttatct ttctgaaatg ggcgtaagta aagaaaaatt
     3061 agcctatgat ttggctcatg gatttaccga tgaaaatccg acaacttcag atgataaacc
     3121 agtcattgat ccaactcgag caggtgctgc aaatcctacc ctgacagatg gaacaaatta
     3181 cgcccacatt gatcagttcg gagaaatcga aaacgcaaac ttacatgtgg ctggttggca
     3241 catcgctaac tataaatacg agtatatctt cattatggat tacaatactg ggaaagaatt
     3301 agctcgagta aaagctgatg gaatatatag accagatgta aatcaagctt ataatacttt
     3361 aggaaatgtt ggctatcatg tatcttttaa catgcgcaac ttccctaata agaaagtcta
     3421 tgtaatgatg cgggcaacga atgatccaga agggaacact aaaggcggag cacaagattt
     3481 tcatgataaa cgctggtatt taaatattcc gcaacgataa aaatagcccc tcgtttttga
     3541 ggggaagtac ataatgcaaa tttgtccata acttattgat tatcttgtaa aaatgcggta
     3601 atttaatagt tagtgagaag aggtgcttaa ttatagttaa aaaagaggaa atagaagcag
     3661 catgtaacca agcaaatgtt aggtttattg gagaagcttt atctggatat cctaagtata
     3721 aatacttaca caactggtta caaagtaaaa gtttttatct taataaagaa agatgcatgc
     3781 ctaataaaaa ttatagggtt tataaaagag gaactattgt atatgttgat tttggggtga
     3841 acgtgggtta tgagttatct ggtaatcatt ttgctattgt tttaaataat aaagataatt
     3901 cgaaaaatgg tgttgtatgt gtagtaccaa tctcttcaaa aaagaaaagt aattatgtat
     3961 ctgtaggaaa aatattagaa gttgctagta ttaaacaatt tataagccaa gctgataaat
     4021 taaaagagaa acttaggata attattttac tttcattaaa tagaggcatc attgatgaaa
     4081 gcgatttgaa tagttttatt aaagatattg aagctaaagg tgaaaagctt tcagtggaag
     4141 aagtagaaaa acgagctgta aaacataatt tggtttgcga gacaagggaa caagtaatta
     4201 aagaaataaa atcattatct gaagatatgg taaaatatca gaaggtgttt aattgctaca
     4261 agaaatatac acaagaatcc tatgtaatgc cacttaatat ccaaacgatt agtaaaaata
     4321 gaattagaag aattaacaag tttgatcctt ctggaaatat gaaggcacct ggaaatgtcc
     4381 tcaatgaagt agatgaaagt ttaaaaaaat attttacaaa gtaaacgata tttttgttga
     4441 catttttctg ttcgttagtt accatgtagt tatagaagga cgtaagcgcc tatttttaat
     4501 gtacacatcg ggacgtatgc gcccacatag aaacatagtt gagagacatc tatatggtgt
     4561 ctcttttttt attaatatgc aaaaaagtct agttttttca ataatattta tgtaataata
     4621 aattagccac cacaacaaag aatgaaatcc attattatct agtctagtcc attctttttg
     4681 tttgcagtat ttgtgatggc tttccgtacc cttagctcag ttggtcagag cagacggctc
     4741 ataaccgtcc ggtcgtactt ctcgtaatcc aggaaggccg tcagctccgt ccgcagtagc
     4801 gtgtttacgg ccagctccag atgggagcgg aataactcgt tcaaatcacc tttggtggct
     4861 agagtctgca ggagttctgt aataaaatcg ttcataggga agtcctcttt tctgtgaatg
     4921 ggtgtggtaa ctttattcta cagaagggac ttcctttttt ctatttacac aaaatatttt
     4981 atactctcaa atgtttgtat gcagttacat ctgactttat tcgaattaag gaaaagattt
     5041 ataaaaataa aataaaaaaa tatatattta aattgactta ttgtgaaatc attaactata
     5101 attagttcag tttatgttaa ggagaatgat gatatgaata agaaaattgt agtaagtgga
     5161 ttaattatag gtggattagt gttagcaggg atcgcaggga aaaactttgc cgatgacatt
     5221 ttgttatggg gtggagaaga taatattgaa caaatcaatc ataatttgga gtcattagat
     5281 aaagcactag aaaataaaga gcaaaatatt agtagtttaa attcacgatt atcttcaaat
     5341 aatcaagaac ttgagcaagc aaaggctaac gttgaagaat ataaacaaaa agtgttgact
     5401 ttggaaaatg ataaaaatca attaactata gagaaaacaa atttagagaa tcaattatca
     5461 ggaaaaaatg ctgaattgca aggtaaacaa aatgaaatca atgctaaaat tgatgaaata
     5521 aatcaaaaaa tagctgaaat aaatcaatta aacactgatt ggaataatcg tttacaaaca
     5581 gaacaaaata aatttaatga tgctcaaaat actgttaata gtttaaatca acaaattaat
     5641 caattgtcaa atgaaaaaaa tactattaaa aaccaattgc aaagtactga acaaaaacta
     5701 gaaaaagcgc aatcagaaaa cagtaatttg aataattata ttgaaaaact ggaaaaagcc
     5761 aaaagcgatg tagaagacac agcaaacaga tcac
//
LOCUS       DADVXU010000132         5779 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.137, whole genome shotgun sequence.
ACCESSION   DADVXU010000132 DADVXU010000000
VERSION     DADVXU010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.137"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..813
                     /locus_tag="KB953_002620"
     CDS             <1..813
                     /locus_tag="KB953_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HBB6867538.1"
                     /translation="KKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSD
                     NGQIKLLWKSPLSTEKNGVSSLTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSG
                     DNFIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIES
                     KSLNQDQIIKYANYCGVYPKKIARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHAL
                     ALFGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            807..1229
                     /locus_tag="KB953_002621"
     CDS             807..1229
                     /locus_tag="KB953_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867539.1"
                     /translation="MVNKLIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2363..3331
                     /locus_tag="KB953_002622"
     CDS             2363..3331
                     /locus_tag="KB953_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBB6867540.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIISRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     NFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKEPTSLQEKIKEQKRKLELL
                     QQLAELERKQNTPSKKINKQIADNNSDITPEFKENLLDNLQNLFKE"
     gene            3341..3829
                     /locus_tag="KB953_002623"
     CDS             3341..3829
                     /locus_tag="KB953_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HBB6867541.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNITYLKKE
                     AVTTITKVLSGNESGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQIALETQ
                     QKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQNKEKKWWQF
                     WQ"
     gene            4125..4406
                     /locus_tag="KB953_002624"
     CDS             4125..4406
                     /locus_tag="KB953_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867542.1"
                     /translation="MDILVRNIDPKVISEIDRRCQELTTKTGKKWSRNAYLKVLIEND
                     FDQALRHYKKDQFDLLLEKFIDIQAKNTETLNDYVHTNNQLIETLIGGI"
     gene            4408..4824
                     /locus_tag="KB953_002625"
     CDS             4408..4824
                     /locus_tag="KB953_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867543.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPNTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSRNFDPTNFLKR"
     gene            5438..>5779
                     /locus_tag="KB953_002626"
     CDS             5438..>5779
                     /locus_tag="KB953_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HBB6867544.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTV"
BASE COUNT         2154 a          980 c          948 g         1697 t
ORIGIN      
        1 aaaaaaaata caaatggaga atattcagga agtatttcac aatttttagc aaaagaactt
       61 caaaatctag tagatagtaa tataacagat acaaccttat tttttcaaga taatagcatt
      121 tattattccg ataatggaca aattaaacta ctttggaaat cacctttaag tacagaaaaa
      181 aatggtgttt cttcccttac agaaaaaaat gatcttatat ccgtaaatat aaaaaataaa
      241 attaaaaatt tttctaaaaa acaaaatttt cctgcggtta aacgtgtaaa aagtgtctcc
      301 tccggagata actttattat gattaattgg cgtatagcag aaacccaaac aagttttccg
      361 tggtgcgctg cttacgtaga agctgctatc ttatgtaaca aaacagatgt acgtgctact
      421 tcagctagag ccattatgca ttatacctat cctaatttaa acaacagtca aattgaatca
      481 aaaagtctta atcaagatca aataataaaa tatgcaaatt actgtggtgt ttatcctaaa
      541 aaaatagcac gaatactaaa cctttcagag gtccaaacac aactaagaaa aggaaatgcc
      601 atttatatgg gaactgctgg ttatggaaat tatagtaaca gccgtcatgc acttgcatta
      661 tttgggtggg tcaatcaagg tggttatcaa acttactatg tgtggaatcc ttggtataac
      721 tacccaactg taataagtgc aaatgcagga ccagttacaa ttcctgtacc tggaggtggt
      781 tttacttggg acaataccat tacaaattgg taaataaact cattgcttta gttatgggtc
      841 tagtgttagt tcttttctta actagttgtt caaaaaaaca agaaatacaa aagactgatc
      901 tgattccttt tctaggaact tggcaaacta aagatcattc agaacgtcta gtaatcacta
      961 atttaaatac ttcaaaaaat atagaacaat ataccatctt agtaaaagag aacaatcata
     1021 aaattcaaat cattgataaa aatttaaata taaatgaaca agttttcatt tacaaagcaa
     1081 caaataattt actaacactg cgctcgaata aaaaacaatt agattacact tttagtttgg
     1141 attctccaaa aaagctcgta tttattttga caacgaatga tgggacagaa gggacagcta
     1201 aaccaattga attttttaaa gctgattgat gaatagttgt ttgatattac aaaacaataa
     1261 tttcaccgaa ggtgaaacaa gcgaaacttt acaaaaagtg gctagcattt gctagccttt
     1321 tttgtattgt ggagcgtggg ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1381 aagcggttat ttgcaccaaa tacgtaggta tttggctagc aaatatagag cttgccaagc
     1441 cctttagtgt gttttgtaga aagggacctt atagtcactt tcacattaca aacgatacgt
     1501 ccataggacg tatcaaaagg gtgggactta tgagcaaatt aaagcaaaat ttggctagca
     1561 atccttctaa aaaatacacc tattgatcag agtcaaaata aatcagtttt cgagtgagcg
     1621 aatccaaatt tgcaactctg aagctttgca aatgagtgtg ccggcttccg taaaagtcta
     1681 ggtaacatta gtcgatatat acatagggct ctaatttgcg ttttaaggcg ttttaaacgt
     1741 tctggtatgt aattatacca aagcgacttt aaaacgcctt aaaacgaatt ttagaggttc
     1801 tgtagaacgt tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg ctttaaaaac
     1861 aagataaggg attttatcta gacttgtctt aaaacgccgt agatgagcgc tcagagcttc
     1921 taaaaggact ccttacgaac tgtttttaag caaaggagcc tttaggcgac tgcgcagttt
     1981 aatgtgagag cgtagcgatc acacctttaa agcgaccgta gggagcagat tcccagttta
     2041 ggaaatttcg cgaacctgtt tggagaaaaa aagtaggaaa attttaggcg ggaaattttg
     2101 cccccctctt ttataatctc ttttataacc tctttttaaa cctctttacc gtcggctgta
     2161 tccttactct cccaaggtgt gcagaatctt ttaggggtct ttttgtctgt ttttaggggt
     2221 ctttttgtct gtttttaggg gtctttttgt ctgtttttag gggtcttttt gtctgttttt
     2281 aggggtcttt aattaggggg ttgaccccca ttagaaagct ctttataatt ttgttaagta
     2341 gttaatcttg aaggaggatt ttatggcaaa tgaaattgtg aaataccacc atgaattaaa
     2401 tacgattcct ttacgcaaat ttacgtcagt agaaatgaat ttattcttct ctataatatc
     2461 tagaatgcgt gatgtaggcg ataaaaaagt acaatttaca ttcgaacaat taaaagattt
     2521 aagcaattat aaagcaactg caaatgttcg attcatagat gatttagaaa caacgtatga
     2581 caaattaatg gatttacgtt ttggtagaag aagtgctgac ggtttgcaac gtgaacgatt
     2641 tgttttattt aatcaattta aaatcgatgg aaaagctgat attccttttg ctgaaataca
     2701 agtccatgag aaagccttac ccttacttaa taatcttgaa gaatgggttc gatattctct
     2761 ccaacaattc aatgaattag aaagcagtta ttccaaaacc atgtttagat tattaaaaca
     2821 atacagaacc aaaggaattg ccaatttttc aaaagaagac tttcatgaat tacttgatat
     2881 ccctaaaagt tatagagaaa gtgatataaa taaaaaaatt ataaagccta ttcgccaaga
     2941 actaaccgca atcttcaaag gtttgacaat ccaaaagaga tacggaaaag gacggggaaa
     3001 gcctgtcatt ggctatcaat ttacatttaa acctgagata aaaaaatcag acgactttaa
     3061 caaacatgct ccaaccaaag aagaacaaaa taaagctttg gaactagcta atcaacaggt
     3121 tcaatctgaa aaagaaccta cttcattaca agaaaaaatc aaagaacaaa aacgaaaatt
     3181 agaactttta caacagttag cagaattaga aagaaaacaa aataccccaa gtaagaaaat
     3241 taacaaacaa atagcagata acaattccga tattacccca gaatttaaag aaaacttact
     3301 agataatctc caaaacttat ttaaagaata aggggttttt atgacagata aaactatcaa
     3361 agaattagca gatattctag gtgtttcaaa agataaagtg aagtatcaac tgaggaaatt
     3421 acctagtaat tttacctaca aaaaaggaaa tattacctac ctgaaaaaag aagcagtaac
     3481 aacaattaca aaggttttat caggtaatga atcgggtaac ttacctagtg aattacccga
     3541 ttcattacct accgattacc tactcaagga gattgaaact aagaataatc atattgaaga
     3601 aaaagatagg caaattgaaa aacttcaaat agctttagaa acgcagcaaa aattactcga
     3661 ccagcaacaa caactcaccc tacaagccaa tcaacaaatc aaaaaacttc acgaacaact
     3721 ccagctaacc tatgcagaag aatcttcaga aaaagaaccc ctttctgtta ctgaagaaac
     3781 tcaaaaagga caaaacaagg agaaaaaatg gtggcaattc tggcaataac ccaacaattt
     3841 ttattcataa aaattgttgg gttactttct ttttttgctt ttacaagcat agcgacatga
     3901 tctcacgtcg ctatttttta aagagcataa ggaaacgcgg tatcactttc ttacgaaagg
     3961 tgctaggtag gctgtgccta ccacgtttct aagaaaaaaa tttgctatca ctttttcgtc
     4021 attgtggata gcttatgtat agtgtttcgg caaaaaacag ctatacaaat actatatttg
     4081 tatgatatac ataaattata cacaaatgcg aaaggaattc ttctatggac attttagttc
     4141 ggaatattga tccaaaagtt atttcagaaa tagatagacg ttgccaagaa ctcacaacaa
     4201 aaactggcaa aaaatggagc cgtaatgcgt acctaaaagt tctaatcgaa aacgattttg
     4261 accaggcact taggcattac aaaaaagatc aattcgattt attactcgaa aaatttatcg
     4321 atatacaagc aaaaaatact gaaacattga acgactatgt tcatacaaac aaccaactca
     4381 ttgaaacact tatcggagga atataacatg agaaaaactt ctgttcaaaa acacgttacc
     4441 ctttatccca gtcacattga cgttttagat tccatcatcg aaaaaaatcc agatatcaat
     4501 aactactcgg aagccattcg ttttctatgt atgagttatg aacctaatac taatacgaca
     4561 actaccagca accaagcaaa actaaatgcg atgggaaaag aaatttctat tatgtccgaa
     4621 atcattgcag aatttgctga tttatatcta aaagatactg gtgttcttat cggtacaaat
     4681 tctaaagtat atcaagaagt gaaagaaaaa attgaaaatc aactcaatcc ctcccgacga
     4741 aatcaaccca aaaatcgtca aaaagaaacc aatcaaacat tcacttctag aaattttgac
     4801 ccaacaaact ttctaaaaag atgaaaaaga aaccgaaaat tttttctatt tctcagtttc
     4861 ttttttaggg ggcagtcttc cagcaagatt tgcaagttac cggtaacttg cacaaaaaaa
     4921 gaaaaatccg cttgtgcgaa tccttctttt tgcattttgg ggcgctgccc caatctccaa
     4981 atatcggtta tttaatagcg tggtaactca aaaaaataga acgtactctg aaataaaaaa
     5041 agactgctct aacgagcagc caataaaaaa taatgagcgt ttattcttga aaaagcagcg
     5101 cgcttatgaa gcgctgccaa aaatttacca tattttatga aaaataacaa gtttctaaaa
     5161 aggaatcggt taagattcac aaagaatcgt aaacagacaa tagcggaaaa tcaagcttcc
     5221 gaacgtacct tttagctaga ccaatgaaaa atcacaaagt gagcggctgg atcggttggt
     5281 agaggggcta cttggcatag tgtacattat gttaactaac gctataaata acgattatgt
     5341 tcccaaaaca atattttatg aactataaca aaaatttgtt atcgcatatt gtatatttta
     5401 attataggtg tataccactt aaatttaaca caaaggcgtg attattttga aacccaaaaa
     5461 gaaaatttat ctaggtctcc tcttttcctt gatgattgga atttttagta taggaattac
     5521 aaatacctat gcttcagaaa attctaattt caatatcaac tcccaaccaa ttgaagaaca
     5581 agcaattaat tttgcgaaaa ctgattttga acactccata acttcaattc ttttaatgga
     5641 aaataacacc gaaactgata ttaactatac tcttggttcg ccatttaaaa tgctcgatgg
     5701 agaaaatacg gcaaatagtt ctattttctt tccagtattt tcagacgctg ataatacgat
     5761 taaatatatt tataccgtc
//
LOCUS       DADVXU010000133         5741 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.138, whole genome shotgun sequence.
ACCESSION   DADVXU010000133 DADVXU010000000
VERSION     DADVXU010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.138"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            100..399
                     /locus_tag="KB953_002627"
     CDS             100..399
                     /locus_tag="KB953_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6867545.1"
                     /translation="MEAIKLMISEEQEEMFLKQFYQIATKAIEKAQENAGMTREYLNQ
                     KEMAEYLNVSENFLKNAVRNEGLPVVELGARKFYSKKAVNEFMFARMKRGENIGR"
     gene            664..1539
                     /locus_tag="KB953_002628"
     CDS             664..1539
                     /locus_tag="KB953_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867546.1"
                     /translation="MNAIVILAEITYWYRPKKIFNDDGQLLGYQKKFLEDILQKSYKQ
                     LSKKTGLSERAIKDAIVYLEKKGIIKRVFRNIYYEKRAVTLSNVMYIVLNVHKLIEIT
                     FPTLDCEKKEYMPKIRQRGIEKKRYVSLKKGRGMYKFVYTYTKTTSQNISKTTLVMEE
                     EDINIKRIYSKISVLYDANNEDGRKLFQVYHYLRNANVDTEDIRKIIEYLSENTQYLI
                     AECIIKQHEACFFQRKTEEGLYDYVTYFLNGLQKRYKSQFLENVEPIDQFKKAFEIKE
                     LPEITMHNWLKGEES"
     gene            1536..1754
                     /locus_tag="KB953_002629"
     CDS             1536..1754
                     /locus_tag="KB953_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867547.1"
                     /translation="MIKNRLIDQMWCLDQNTDTLELIESICFNTVVLDLRAENDNCVT
                     HIIINQKMAQQLSESLRKWAEGENYESN"
     gene            1741..4368
                     /locus_tag="KB953_002630"
     CDS             1741..4368
                     /locus_tag="KB953_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867548.1"
                     /translation="MKAIKEKIVSRKINWLALSVLVLMISVVFTSNIVEASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSVINTIVVGLMILSLMFVGYKMITGKGTIDLRSVGMNIVMSVALI
                     LLMPTMISSGIQFAKIFYNDATTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNKLTRKNFQQTDLTQVLTDKVIDKLEKENPAADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNAGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSTGFNRAKGSGN
                     GNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERAIK
                     PFKSMRDMANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKS
                     AEELINQKRQEAKYTPEAMNREEMLRKRVEGRTGANTDEKEALTKERIQEAKNSNTGL
                     EKLVKENLQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIDV
                     RENVQNSTSSQGFKSMDVRENVQNSTSSQGFKSMDVRENVQSSTSSQGFKTVDVRENV
                     QKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKPRRRFTYEDNE
                     LFRDTLNDPNPLFDNLLKK"
     gene            4383..4601
                     /locus_tag="KB953_002631"
     CDS             4383..4601
                     /locus_tag="KB953_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867549.1"
                     /translation="MKLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKHEIKNNLKYCYLEKSEEIIHELNGF"
BASE COUNT         2276 a          714 c         1109 g         1642 t
ORIGIN      
        1 taaatacaaa taaatacatt tttttacgca atttgtattt atttgtactt ataaaggtag
       61 aggagttata gaataattaa ttaaaaagga gaggtttttg tggaagcaat aaaattaatg
      121 atttcagaag aacaggagga aatgtttcta aagcagtttt atcaaattgc tacaaaagct
      181 attgaaaagg ctcaagaaaa tgcggggatg acaagggaat atctcaatca aaaagaaatg
      241 gcggaatact taaatgtgag tgaaaacttt ttaaaaaatg ctgtgagaaa tgagggattg
      301 cccgtagttg aattaggggc aagaaaattt tattcgaaaa aggcagttaa tgagtttatg
      361 tttgctcgaa tgaaaagagg ggaaaatatt ggaagatgat gaagtagaaa ttataaaaaa
      421 atgctttaaa aaaagaaaaa aagtgttccc aaaagtttgc agactggggg aacactcaat
      481 aataaagtgc tgagaaaact ttatttatag ctattatatc aaaaaagaag gtgaaaaggt
      541 gttttcttct ggaaatgttg tagttgacaa acttagagaa ataaattttg acggaaatgt
      601 aatccacaca catggtacga tcaattaaga aaaaaaacga aaaaaggtgt tgagaagccc
      661 tatttgaatg caatagttat cttagcagaa attacctatt ggtatagacc taaaaaaatt
      721 tttaacgatg atggtcagct acttggttat caaaaaaagt ttcttgaaga tattttgcaa
      781 aaatcatata agcaattaag caaaaaaaca ggattatcag aacgagctat aaaagatgca
      841 attgtgtact tagagaaaaa ggggattatc aaaagggttt ttagaaatat ttattatgag
      901 aaaagagcag ttacattatc aaatgttatg tacattgttt taaatgtaca taaattgata
      961 gaaattactt ttcctacttt ggattgcgaa aaaaaggagt atatgccgaa aattcgtcag
     1021 aggggtatcg aaaaaaagag atatgtctct ttaaaaaaag gtagaggtat gtacaaattt
     1081 gtatatacgt atacaaagac tacttcacag aacatatcaa agactactct cgttatggaa
     1141 gaagaggata ttaatataaa gaggatttac agtaagatta gtgttcttta tgacgcaaat
     1201 aacgaagatg gtcgaaaatt atttcaagtg tatcactatt tgagaaacgc taatgtagat
     1261 acagaagata tcagaaaaat cattgaatat ttatctgaaa atactcagta tcttattgct
     1321 gagtgtatta taaaacaaca tgaagcatgc ttttttcaaa gaaaaactga agaagggtta
     1381 tacgattatg taacatattt tttgaatgga cttcagaaaa gatataaaag tcaattttta
     1441 gaaaatgtag agcctataga tcagttcaaa aaagctttcg aaatcaaaga gttacctgaa
     1501 attactatgc ataattggtt gaaaggagaa gaatcatgat taaaaatcgc ttaatagatc
     1561 aaatgtggtg tcttgatcaa aatacagata ctctcgaatt aatcgagagt atctgcttca
     1621 acacagtagt tctcgattta agagctgaaa atgacaattg tgtaactcat atcataatta
     1681 atcaaaaaat ggctcagcag ctctctgaga gcctaagaaa atgggctgaa ggagaaaact
     1741 atgaaagcaa ttaaagaaaa aatcgttagt agaaaaataa actggttagc actcagtgta
     1801 cttgttttaa tgattagcgt tgtttttaca agtaatattg ttgaagctag cattttcaat
     1861 tggtttgatg acacaaaaag tcaaacagag tttttgaata attctcttta tagaccttac
     1921 ctaaaaaaat acaatggaga tttccttagt cagttcggga tttggcttgg atgggctgtg
     1981 gtaaaaggca tgtttactgt gactgattcg attcaaaata tgattccaga tgtgttggat
     2041 ttgttcaatt tcattgaaag tacagggcta aataatgttt atcaatcagt cataaataca
     2101 attgtcgttg gattaatgat cctttcattg atgtttgttg gctacaagat gattactgga
     2161 aaaggaacaa tagatttaag atctgttgga atgaatatag tgatgtctgt agcgttaatt
     2221 cttttaatgc caacaatgat ttctagcggt attcaatttg ccaaaatatt ttacaatgat
     2281 gctactacga ttacaaattc agatgacgga gtcgcttggt cattgataaa acaaggagta
     2341 acagatctgg catatattaa taaaacagat caatactcaa gcattgataa aacggaggat
     2401 cgaaataagt taacacgaaa aaattttcaa cagacagatt taacacaagt gttgacggat
     2461 aaagtgattg ataaattgga aaaagaaaat ccagctgcgg ataatcttcg ctatgaatta
     2521 gttgaaaatt ctaataatga atttgttgcc acaaagtttt ctgataactt cttatcaaca
     2581 ttttctgata gcttgaaatc aggttattat cgatatcaag caaatctttg ggggatttcg
     2641 attgggttga cagcgttggc aatcgcctat gtgttcagcg cgtttgtgat cattacagca
     2701 atattagaac ttgcatttaa acgtgtgtta ggtgttcttg tttttgcgac agatattgaa
     2761 acaggacaac gttcaaaagt tgttttatcc gatattttac aatgttactt aacagttgga
     2821 tttcaagggt ttggactatc tatgtttgct atgtttatca attttttaaa tgctggacaa
     2881 ggtatctcca caaatatttt tataaaaaca attgcttata tctgtgctgt gtttgtgctg
     2941 ataaaaggca gtggaacagt catgagatac tttggtgttg atattggact caaagaaggc
     3001 tatgggcaac ttgcttctgc ttttggtatg ggcgcaatgc tgtttagaaa aggatcaact
     3061 ggatttaatc gtgctaaagg aagcggaaat ggaaatggat cagacagtgg agaaggagaa
     3121 gatcgaaaac cagaaaagaa tttcggcgaa actctatcaa aaaaagctgg caaaactgga
     3181 agagctttgg gatatgccca tgaacgtgga ttatcaggtt tagcttcaga tggagctaca
     3241 atggctagtg aaagagcaat aaaaccattt aagagtatgc gtgatatggc taatgatacc
     3301 aagaataagt tcaaagaagg tttagatgat ggtacagtgt ccgctattaa taaaaatagc
     3361 aaacctatgt tagctaaaaa taaagaagat gaaactggaa aatatgctga ttccatgcca
     3421 acacgtcttt cggatagaaa agatggtgct aaggttgaaa atgccgatag aattatgtct
     3481 agttctgaac gtatgcgtga agcaatgaaa aataatgctg aatcgaataa taatcctgta
     3541 agtgcaattc agcaaaaagt tcaacaagat attgaagaac gtaaaaatgc tatgcacggt
     3601 caagctaagt ctgctgaaga attaattaat cagaagcgac aagaagctaa atacacgcct
     3661 gaagcaatga atcgtgagga aatgttaagg aaacgtgtag aagggcgtac aggagctaat
     3721 acggacgaaa aagaagcgtt gaccaaagaa agaatccaag aagctaaaaa ctcaaatacc
     3781 ggattagaaa aattggtcaa agaaaatcta caaaattcta caagtagtca aggtggccga
     3841 tcagtagatg tccgagaaaa tattcaaggt tcattgaatg gtcatgtcgg acggaatgtt
     3901 gatgtaagag agaatttaca aagctcttca aatggtcaag gtactaagac aattgatgtt
     3961 cgagaaaatg tacaaaattc tacaagtagt caagggttta aatcaatgga tgtaagagag
     4021 aatgtacaaa attctacaag tagtcaaggg tttaaatcaa tggatgtaag agagaatgta
     4081 caaagttcta caagtagtca aggattcaaa acagtagatg ttcgggagaa cgttcaaaaa
     4141 gatagtggag aaatgaaaga aaaaacacaa aaaatcaata tcgttgaaga cagaaagtca
     4201 gcgaaaaaat ttgattcaaa tcatgaaaca gtgattattg atagtgaaat ccgtgaaaat
     4261 gataagggat caaaaccaag acgaagattt acctatgaag acaacgagtt attcagagat
     4321 acacttaatg atcctaatcc gcttttcgat aatctattaa aaaaataaaa gggggaattg
     4381 tcatgaaatt aattctaaag attagtacgc atgatttctt agaacttgac gaattatgtg
     4441 aatcagtaaa gaacgcaaca aatgaaaaaa tcacggtcac taattatatt gaactgatga
     4501 ttcaagaaga attaaagatg aaacatgaga taaaaaacaa cttgaaatat tgttatctag
     4561 aaaaaagtga agagataatc catgaattaa atggctttta aaaattgtat ttgacatgaa
     4621 tttacgacaa ggaattagtg gggattaagc gattattctc ttccacttta gagaaataat
     4681 gggttaaaaa gccagaatac gtagagcaat attgacaacc tataaaggag ctattgttga
     4741 aaattgaagt aacaaaaaga taaagggagg tttacatcta tcagattaaa agtgatagta
     4801 cacccatgaa acctgcaaaa gagttgaacc taatgtatat cggtttaact ctttttattt
     4861 gcaaattaaa aaaatatcac cgaagtgacg aatgaaacta tgaccaaatt aagcaagaaa
     4921 atatatcttg caaataaagt agtataaaaa gatattgata aagccaatca aaactttgaa
     4981 tttcaaacgt tagataatca aacagataca aaaaatgaaa tcagttctac gcgtacgatt
     5041 attaaaaata ataaattttc actagaagaa aatggaaata aaatcaaata attaggaaga
     5101 aaaaacaaag caattagaaa agcctgcaag caaatttgag taatcgagat ccaatttttt
     5161 taaggaagtt taacaaaaat atgtaattct attatcacta ttaaagagaa agttggagcg
     5221 gaatataaag aaaagagaaa agtaattaaa cgcaaaaaaa tgtagaactt aaaaaatata
     5281 aagaggaaca accgttaaaa ttaggaatga tgttgtgtaa acattataat tgctatgata
     5341 taaatgaagt gttaaagaaa gcaaatttaa accaattttg tgaacagaaa agccttagtc
     5401 actatgacca aaactacgga cgaaactatg accaaaagta ggtactaaat tatgaccgaa
     5461 aataggtcca aaatggattt ttttatgata tcaaaaatta tgaaatgatt ttgaaagaaa
     5521 accagttggc acagccaact tagccacctt cctgtaggaa ggtgctatca gttttccctt
     5581 atgctctttt ctttcttttt tgtttcatat gtcaaatgcc attttgaaat gtaccatctt
     5641 aagaaattta tccttaaaaa caattgcgtt attggataaa aagatgctac attatcttaa
     5701 aaaaggaatc aaatatgatg aatttagtgg ctagcatatc g
//
LOCUS       DADVXU010000134         5662 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.139, whole genome shotgun sequence.
ACCESSION   DADVXU010000134 DADVXU010000000
VERSION     DADVXU010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.139"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            512..1069
                     /locus_tag="KB953_002632"
                     /pseudo
     CDS             512..1069
                     /locus_tag="KB953_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            1127..3166
                     /locus_tag="KB953_002633"
     CDS             1127..3166
                     /locus_tag="KB953_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HBB6867550.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDQYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGEKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKDDDDSATKRASELLQYLNQNYQ"
     gene            complement(3275..4426)
                     /locus_tag="KB953_002634"
     CDS             complement(3275..4426)
                     /locus_tag="KB953_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBB6867551.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVFLLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4580..5110)
                     /locus_tag="KB953_002635"
     CDS             complement(4580..5110)
                     /locus_tag="KB953_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBB6867552.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(5209..5559)
                     /locus_tag="KB953_002636"
     CDS             complement(5209..5559)
                     /locus_tag="KB953_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HBB6867553.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
BASE COUNT         2061 a         1035 c         1053 g         1513 t
ORIGIN      
        1 tataaaaaaa cacataaaac taaaaaatta tcaaagaatc ctggtgcaac tgaaagaatt
       61 tgcaacggga ttctttcaac ttatttgtta tttttttcta aaaaatatta aagattgtta
      121 aatttcacta tttcatttca aattccattg cctaatcatt aggaaaagtc tatcatgaac
      181 tcgtaaatag aatatagtaa taaccttgtt aggtgaggct cctatacaaa cataggctgt
      241 tgcccagaaa cgtcgaaagt cgccaatggg taaaacagaa actgttggat taaggctttt
      301 tccaatagct aagaatgttt tctttacgtt gtatagtgct aaaactcgga cgatgagatg
      361 atgctaggtc agaattcttg ttcgaaaagg tttctgacct tttctttata tttttgtgac
      421 tgacttattt gatgagacaa actcgctcct cacctgacaa gtggattgct gtgtgaggag
      481 gagatgcggt gaaaaaagat cgattggtgg aggtcgctcg gattcacttc tggttgcaca
      541 atacaaccag aaaacacaac aagcaaaact gatctctatc atgagagatt catatgtcga
      601 tattccaggt tacggaatga ataagataaa tgcagcttac tcttatgggg gagttgattt
      661 attaaatcaa acattaaagg aaaatttcaa atttgaagct ccctattatg caagtatcac
      721 atttcaagat tttatcgatt gcgttaatga actgtttcct ggtggagtca agatcgatgc
      781 agaaaaatct ctagatttag atggcgtata tataaacaaa ggagagcaag tgatggatgg
      841 taatacttta ctccagtatg ctcgatttcg ggaagacaaa gaaggagact tcgggaggat
      901 cagaaggcag cagcaagtga tcaaagctat ctctcagcag ctgaaagacg taacttcaat
      961 attcaagcta cccaaagcgg ttgggaaatt acttggtagt atccaaacga accttccaga
     1021 aagtgtgctt ctggattgtg gtatggattt cctcaaagac atatgttaga ataaacaggt
     1081 ataaatagtg aaataaagga atgacaagca agagaaggag gaaaaaatga aaagaagtga
     1141 caagcacggc aaaaatcgaa caggcgctta tattgccggc gcagtgatct taatagcagc
     1201 tgcgggtggc ggttattttt actaccagca ctatcaagaa acccaagcag tagaagctgg
     1261 agaaaagaca gttgagcaat ttgtccaagc tttgaacaag ggagattata acaaagctgc
     1321 agaaatgaca tcaaaaaagg cggcaaataa aagtgcatta tctgaaaaag agatcttaga
     1381 tcaataccag aatatatacg gtgctgctga tgtcaaagga cttcagatat cgaatctaaa
     1441 agtagataaa aaagacgatt ctacttacag tttttcatat aaagcaaaga tgaatacctc
     1501 attaggtgaa ttgaaagatc tttcttataa aggaacatta gacagaaatg atggtcaaac
     1561 cacgatcaac tggcaaccca acttggtttt tccagaaatg gaaggaaatg acaaagtaag
     1621 cctgaccaca caagaagcag caagagggaa tattatagat cgaaatggtg aaccattagc
     1681 aacaacaggc aaactaaaac aattaggagt cgttccaagt aaacttggag atggaggcga
     1741 aaaaacagcc aatatcaaag cgattgcttc ctccttcgac ttaacagaag atgctatcaa
     1801 tcaggcgatt tcacaaagct gggtacaacc cgattacttt gtcccattga aaatcattga
     1861 tggagcaacg ccagaacttc cagctggagc taccattcaa gaagtagacg gcagatatta
     1921 tcctttgggt gaagcagctg ctcagctgat tggttacgtg ggagatatca cagcagaaga
     1981 tattgataag aatccagaat taagcagtaa tggaaaaatc ggtcgctcag gtttggagat
     2041 ggcttttgat aaggatcttc gtgggacaac aggtgagaaa ttaagcatta cagatgcaga
     2101 cggtgtagag aaaaaagttc tgatcgaaca tgaagttcaa aatggcaaag atatcaaatt
     2161 gacaatcgat gcgaaggcac aaaaaacagc ttttgacagt ctaggaggaa aagctggatc
     2221 aactgttgcg acaacgccaa aaaccggtga tcttcttgcg cttgctagct ctccaagcta
     2281 tgatccaaac aaaatgacaa acgggatctc acaagaagat tacaaagctt atgaagaaaa
     2341 tcctgaacat ccattcatca gccgatttgc gacaggttat gctcctggat ctacgtttaa
     2401 aataattaca gcagcaatcg gtctcgacaa cggcactatc gatccaaatg aagtgttgac
     2461 gatcaacggg cttaaatggc aaaaagacag ttcttgggga tcttttaaaa caacgcgtgt
     2521 cagtagtgat gtatcacaag tagacttaaa aactgctttg atctattccg ataatatata
     2581 tgcggcacaa gaaacgttga aaatgggtga gaaaaaattt cgtacaggct tagataaatt
     2641 catttttggt gaagaccttg atttgccaat tagtatgaat ccagcacaaa tttctaatga
     2701 agatagcttt aactcagata tcttgctagc tgatactgga tatggacagg gcgaacttct
     2761 aattactcct atccagcaag cagcaatgta ttctgttttt accaacaatg gcacacttgt
     2821 ctatcctaaa ttgattgcag ataaagagac aaaagataag aagaatgtca tcagcgaaac
     2881 agcagtacaa acgatcgtgc cagatctgag agaagttgtg caagatgtaa atggtacagc
     2941 acattctctt tctgctttag ggattccgtt ggcagcgaaa actggtacag cggaaatcaa
     3001 agaaaaacag gatgtaaagg ggcaagagaa cagtttcttg tttgctttca atcttgataa
     3061 ccaaggttat atgatggtca gcatgttgga aaataaagat gatgatgatt cagcaaccaa
     3121 acgagcatcg gaactattgc aatacctcaa ccaaaattat caataaaaaa ttgcttttta
     3181 agtacagtaa aaggactgtg gcgttatcgt cacagtcctt ttttcaaata aacggttttc
     3241 acgatgtctc tttcacaact tctttttgat ttactcacac ttcataaact ttttttgtaa
     3301 aatatttgag aacaagtgga gaaataagtg tacttaataa aatcacgata actaaaggtg
     3361 aatagtactg tggttcgatc aattgacttt gctgtccaat ttgtaaaatg atcaaagcca
     3421 tttctccacg cgaaatcata ccagcaccga ccatcaatga actatgttga gaaaaaccgg
     3481 caatctgagc accaaaataa cctccaagca atttggtcaa aatcgctaca agcgtcaata
     3541 tagaaataaa gagtagttgt tcactaaagt cagaaaaatc aacttctaat cctacactga
     3601 caaaaaatac tggtataaat actgcatatc ctaaagcctc tacattgtgg tatacttctt
     3661 ctttaacctt tgtctgactg acagctatcc cagcaaaaaa agcaccaatg acagagctaa
     3721 gcccaacaag atccgctacg taagacattc ctaaacaaat aactagcgat acgatgataa
     3781 tagaagaatt tgcgtacact ttttctgcaa atgaaagtag gaaaggcgca atccatttga
     3841 ctaataagaa aataaaaaca aaataaatta gctgttcaat caaaacaagc ggtaaagaag
     3901 cattgctaga agcttccccc gttagaaaag ataggctgaa acttagaact aaaacgacta
     3961 aaatgtcatc taccacagaa gcacccaaaa ttgttgatcc ttcttttgta ttcactacgt
     4021 ttagttcttt taatacttct actgaaatac tgacagaagt tgccgcaaga ataatcccaa
     4081 agaaaatcgc ttcatttccc gaaacttgga atgctttgcc tgtcaaccag ccaaaaacaa
     4141 ccgggaagat gatcccaagc aaagcaacga acataccagg tcggaaatat tttttcaaaa
     4201 gagataaatc actttccaga ccagctaaaa acattaataa gaacacacca atctccgaaa
     4261 agtcatggac tagaatatca ggtttgaccc aattcaatcc agcttggcct agcaaaacac
     4321 caacaagtaa ttgtccaatc actgctggga tacctaatct ccgcgagata tgtgagccaa
     4381 tcgttgttga aatcaatatc aggcataaaa taccaataaa ttccattctc atcattcctt
     4441 tgtataaaat atagcccgaa cttagcataa catacattat tgaaaaaata ggaaaatata
     4501 ctcacaaaaa aaccaacaat ccaaaagatt tttttggatt gttggttttt atacggtcta
     4561 ttttaataac taaaaatgat taacgtatag tcatttttct tattctgatg aaattgataa
     4621 tctcctggat gcccatgaaa atcaaaataa agccgaatat ttggaataga agcatcaaac
     4681 ttttgaatgg attgaacaat aagacgaatc cgccaatcaa caacagaata ctactataaa
     4741 cactccaact tttagctttt gtattcatgc tgaagaacaa ctggaacaag ctgttgatga
     4801 taacagctaa tccaagaaga atcggcagga tagaaacgat tgcaggtgca aaagcataga
     4861 cgatcagagc taagatcagg aaaaaaagac cactgaacag accgatactc caagaacctg
     4921 ttttcctttt aaaacggtaa gcctcaaaaa gattcaacaa cccaagcaaa acaagatagg
     4981 cggtgatcag ataaccgata aaatgaaaaa cagcagaagg gtttagcaca atcgcaatcc
     5041 cagaaataat ataaacagca gctcttaata aggcgtattt ttgaatattt tgtttaatcg
     5101 tctcaaacat ataagaaaac ctcctttgac ttattttaac gtgaactagt cattttacca
     5161 aattaaatgt tacaaaatga gcgaaggaga agaatatctc tggaaaaatc aacttctgag
     5221 gatcttttcc atttttttgc cttttgctag ctcatcgatc attttatcta aataacgaat
     5281 ttctttcatc aagttatcct cgatgtcttc tactcgtaca ccacaaacaa cacctgtaat
     5341 caaggaacgt gaaggattgg ggacaggggc ttccgcaaaa aattgttcga aagtcacttg
     5401 tttttgtagt tgatattcta gttctgtttg ggaataaccc gtcagccaac aaatgatttc
     5461 atctacttcc tcttttatac gatcttttct ctccgctttt gctacataca taggataaac
     5521 gcttgcaaaa cttgtcgtaa aaatacgatt ttgcgccata atcagtcacc tttctttttt
     5581 tcaaattatt tactacttat agtatataag aaaaagagaa gaaaaagcga ttattcaaaa
     5641 aaacaaaaag gattgtgaca aa
//
LOCUS       DADVXU010000135         5564 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.140, whole genome shotgun sequence.
ACCESSION   DADVXU010000135 DADVXU010000000
VERSION     DADVXU010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.140"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            69..887
                     /gene="purR"
                     /locus_tag="KB953_002637"
     CDS             69..887
                     /gene="purR"
                     /locus_tag="KB953_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HBB6867554.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            1390..2763
                     /gene="glmU"
                     /locus_tag="KB953_002638"
     CDS             1390..2763
                     /gene="glmU"
                     /locus_tag="KB953_002638"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBB6867555.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            2857..3828
                     /locus_tag="KB953_002639"
     CDS             2857..3828
                     /locus_tag="KB953_002639"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBB6867556.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      3834..3936
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4002..4571
                     /gene="thiT"
                     /locus_tag="KB953_002640"
     CDS             4002..4571
                     /gene="thiT"
                     /locus_tag="KB953_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HBB6867557.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(4652..5425)
                     /locus_tag="KB953_002641"
     CDS             complement(4652..5425)
                     /locus_tag="KB953_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867558.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
BASE COUNT         1823 a          983 c         1167 g         1591 t
ORIGIN      
        1 gctcgaattt ttgaagaaaa acttatataa tgtaaaacga taaaatttct taaaaggagt
       61 tggaataggt gaaagtacgt cgcagtgaac gtctagttga catgacacag tatttattga
      121 atcacccgca tgaattgatt tccttaacaa gctttgccga acgttatgaa tctgctaaat
      181 cttctatcag tgaagatttg ggaatcatca agaaaacatt taaagaacga gggtatggaa
      241 ccttggaaac aatcccgggt gctgcaggtg gtgtattgtt catccctgaa atttcatatg
      301 aagaggcgaa aaaatatatt gaatctctag cagaacgtct atcagaacaa gaccgtctgc
      361 ttccaggagg atatgtttat ctctctgatt tgttaggaga acctgaactg ctgcgtcaag
      421 ttggaagaat cattgcttct aaataccttg gtgaaaaaat cgatgctgta atgactgtag
      481 ccacaaaagg cgtacctatt gcccaagctg tgtcctatta tctgaatgcg ccattcgtta
      541 ttgtacgccg ggactcaaaa ataacagaag gatcaacagt aagtgtcaac tatgtgtctg
      601 gatcttctga aagaattgaa aagatggaac tgtctaagag aagtttgaaa cgtggttcac
      661 gtgtccttgt agtggatgat ttcatgaaag gcggcggaac agtcaacgga atgaagagct
      721 tgattgaaga attcgaatca gaacttgttg gcatcactgt atttgccgaa tcaaaattca
      781 acggtcatcg cgccatcgat gattatacgt cactgcttta tgtcaaagat gtagatactc
      841 aaacgaaaaa tattacggtt gtgccaggaa attatttttc agaataagag gttgtgaaaa
      901 agccgcttag tttcgagtag taagaaaaat tttgtgaaaa tggctttcca cattttttca
      961 taaatttggc ttaataccga gaaactggct tttgaacacc gtttatctag aggttgtgaa
     1021 atcagcgtcc tgtcctgagt attagctcaa taaacggaaa aattgctcct catattttgc
     1081 cgtttattga gctaataccg aaggtcagct gattgaaaaa cgtttaatga agaggttgta
     1141 agcctaccgt ttaactctaa gtcacaagaa acgatttcaa aaaacagctt ttcatgtttt
     1201 atgagtagtg tgacttgtga ccgaggagtt ggcaggagag caccggttac caaatagata
     1261 ggcccgttat ggtctatcta tttttttatc cagaaaacgc tgggataaaa gaaaatcact
     1321 tgttttgttg aaaaaatggg tatactggga taaactgaaa tcagcaaact atatttagga
     1381 gtgacgcagt tggacgcacg ttatgcaatt attttggcgg cagggaaagg tacccgcatg
     1441 aagtcaaaat tatataaagt tttacatccc gtttctggta aacctatggt agaacatatc
     1501 attaatcggg tcagtgaaac gaaaccagat gaagtgatta cgatcgttgg acacggagca
     1561 gaacaagtta aagcacaact aggagaacgc agtaaatatg cgttacaagc agaacaattg
     1621 ggaacaggac atgctgtttt gcaagctgct tctttcttgg aaggaaaaaa aggaacaacg
     1681 ttagttatca gtggcgatac accattattg actactgaaa cgctgaacaa tctttttgaa
     1741 tatcaccaag gaaaaaatgc aagtgcaacg atcttgacgg cacaagcaga aaacccaaca
     1801 ggatatggac gaatcatccg tgaccatatt gggatcgtag aaaaaatcgt ggaacaaaaa
     1861 gatgcaacac cagaagaagc tcttgtacaa gaaatcaata caggaactta ctgttttgac
     1921 aacgaagctt tgttcgatgc gttaagcaaa gttggaacaa acaatgctca aggtgaatac
     1981 tatctaacag atatcatcga aatcttaaaa gaagaaggcc atacagttgc tgcttatcaa
     2041 acggatgatt ttgaagaatc aatgggagtc aatgatcgga tcgcgttagc taaagcaaat
     2101 gagatcatga gaaagcgaat taaccagatg catatggtca atggcgtaag ctttgtcgat
     2161 tcagcaacga catacatcga tgcaggcgta gaaatcggtc cagacacatt gatcgaagca
     2221 ggtgtacaaa tccaaggaaa tacagtcatt ggttctgatt gtgtcatcgg gtcacattca
     2281 aaaatcgtag acagtaggat tgaggatcac gtcgtcattg aaaactcagt cattgaaagc
     2341 agccatgtca agaaacatgc agatgtggga ccttatgcac atttacgacc aaaagctgaa
     2401 attggcgaaa acgtccatat cggaaacttt gtggaagtga aaaatgctca aatcggcaaa
     2461 ggaactaaag tcggtcactt gacttatgtt ggtgatgcta cgctgggaga agaaatcaat
     2521 gtcggatgtg gtgttgtctt cgtcaactat gatggaaaga acaaacatca tacgactgtc
     2581 ggcgaccaca gctttattgg ttctagcacg aatatcatcg gacctgtcga ggtagctaaa
     2641 aacagttcga ttgctgccgg gtcaacaatt acagacaata ttccagaata tgcgctggca
     2701 attgcgagag caagacaagt caataaagaa ggatatgcaa aaaaactccc ttatctaaac
     2761 taagactttt gcaaaatcac ttgatttatt taaaaaatat gactactatt tattaggtag
     2821 aattctagag ttgtgaaaaa gaaagtggag gtccccatgt caaaacatta ctttgaccca
     2881 agattaaaaa tttttgcgtt aaattccaat cgcccattag cagagaaaat tgcagcagca
     2941 gtcggcgtcg agttaggaaa atcatctgtt actcaattta gtgatggaga aattcaagta
     3001 aacattgaag aaagtattcg tggatcgcat gtctatgtta ttcaatcaac gagcagccca
     3061 gtcaatgata acttgatgga gttgctgatc atgattgacg cactgaaacg agcaagtgca
     3121 aaaacaatca acgtagtcat gccttattat ggctatgcgc gtcaagaccg taaagcacgt
     3181 gcacgggaac caatcacagc gaaattagta gcaaacatga ttcaaaaagc tggtgcaaca
     3241 agaatgctga ctttggacct tcatgcagtc caaatccaag gtttctttga tattccagtc
     3301 gatcatttga tgggtgcgcc acttattgcc aattatttct tagaaaaagg aatcaaaggc
     3361 gatgatgttg tcgttgtttc accagaccat ggtggtgtca cacgggcacg taaattagcg
     3421 gaatttttga aatctccgat tgcgatcata gacaaacgcc gtccaaaagc aaatgttgca
     3481 gaagtaatga atatcattgg acaagtcaaa ggcaaaactt gtgtactgat cgatgatatg
     3541 atcgatacag caggtacgat cacattggct gccaatgctt tgaaagaagc tggtgcaact
     3601 agtgtgtatg cttcatgtac acatccagta ttatccggcc ctgctttaca aagaatagaa
     3661 gattcagcta tcgagcgtct agttgttaca gactcgatct atcttccaga agatcgtaaa
     3721 atcgacaaaa tcgatgaagt aagtgttggc ggactaatgg gagatgcaat caaacgtatc
     3781 catgaaaaca aaccagttag tccattgttt gaaacaaaaa ataaataata aataaactca
     3841 caaaggggag tccaattggg ctgagattga atctatttct aaacccttcg tacctgtatc
     3901 ggttatgcga gcgtaggaat tgtgaataaa cagctgtttg actattttcg aaagctgctt
     3961 cctcctttgt gactttttta ttcaaaggag gaagtttttt tatgggaaaa aatagagttt
     4021 gggtagaagg aacgattgtt gcagcgttag cgatggtgtt gtcgttgatt ccgatccaga
     4081 tcggcagcag tttttctatt tcattagggc agattccatt gactcttttt gcgttaagaa
     4141 gagggacaaa ggcagggatg ctggcaggtt tcatttgggg catcctacat tttccgttgg
     4201 gacaagttta ttatttaagt gtcgttcaag tgttgattga gtacccgatt gcctatactt
     4261 ttgcaggcgc agcagggctc atggcttcaa atgtccaaaa aggctatgta gacgataata
     4321 cgaaacaaat tagaaaaagc atcgtgtttg gtgcattgat tggcgcactt tccagatatt
     4381 tttggcattt tattgcagga gtggttttct ggggagcgta tgcactatgg gggatgaatc
     4441 cttggctctt ttctttcgta atgaatggtg ccagcgggtt gagtacagct attgtaacga
     4501 tcatcgtttt attagggatc caacaaacga ctccttcgat ttttgtgcca gataaacgac
     4561 aggagctgta aataaaaaag aagcaagaaa cttgagacag aatcatgtcc tagtttcttg
     4621 tttccttttt attttttgat aagaaataga tctacttcaa tggcagataa gaaagatcgg
     4681 catcttctac caattgccag tgtcgatcgg tgacagagta gattaaagta gcgatgattt
     4741 cctcttctcg ataccgggtg ccttttaatt tccaaatata atcttcaatc acatagattg
     4801 ggatacgata tttaccatcg atcaaaccaa cttgatcaaa agaaaaaatg atcccttcct
     4861 gcatcaaatt acgcatttct ttcaataatt ccacactgat tttttcaaca ggtgtttctt
     4921 ttttgaaaaa agattttttt acattgatct tatctgacaa gatcgaactt aatttactag
     4981 caaaaatcct ataaaattca tctaaatagc gataataaat ccgattcagc tgatcattaa
     5041 gatcgaagta tacaaaatta ttctgtagtt tataaaaaaa cggcgaatga agatgtgttt
     5101 tcatatgacc aaaatataat aattctgaaa tttctaaagg tgtcaattct ttcagcatcg
     5161 ttaaatcagt aaagtcgatc cacttcaatt catcgaatcc acgcctgtta cgagaagagg
     5221 aaaagaaacg ctgaatattt tctgtaccac ggattacttt cagccctgta tgggattcat
     5281 attctccagc agaagcagat ggatcaagca agagcaagtt ttgaggaaaa tgcacgatac
     5341 tgtttgcaaa gtcagtagca gtcagccctt ttgttaaaac agcattactt gtggtatcaa
     5401 tatgtacata aactgactca gtcatcatgt tcctcctctt atttcctctt aatctctttt
     5461 attttacaac atttgcatta aaaaagcttg tttcttttat tttaaaattc cattacaaaa
     5521 tcacgttttc attgaaaaca ctctcatttt caattaattt ttgt
//
LOCUS       DADVXU010000136         5304 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.142, whole genome shotgun sequence.
ACCESSION   DADVXU010000136 DADVXU010000000
VERSION     DADVXU010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.142"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(66..1214)
                     /gene="nagA"
                     /locus_tag="KB953_002642"
     CDS             complement(66..1214)
                     /gene="nagA"
                     /locus_tag="KB953_002642"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBB6867559.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1290..2126)
                     /locus_tag="KB953_002643"
     CDS             complement(1290..2126)
                     /locus_tag="KB953_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6867560.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2310..3527)
                     /locus_tag="KB953_002644"
     CDS             complement(2310..3527)
                     /locus_tag="KB953_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBB6867561.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3676..3855)
                     /locus_tag="KB953_002645"
     CDS             complement(3676..3855)
                     /locus_tag="KB953_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867562.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            3910..3993
                     /locus_tag="KB953_002646"
     tRNA            3910..3993
                     /locus_tag="KB953_002646"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3944..3946,aa:Leu,seq:gag)
     gene            complement(4233..4811)
                     /locus_tag="KB953_002647"
     CDS             complement(4233..4811)
                     /locus_tag="KB953_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867563.1"
                     /translation="MNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNT
                     MGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGI
                     YLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKE
                     LMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
     gene            complement(4817..5200)
                     /locus_tag="KB953_002648"
                     /pseudo
     CDS             complement(4817..5200)
                     /locus_tag="KB953_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1652 a         1081 c          838 g         1733 t
ORIGIN      
        1 agaacacaaa aaaaacctta aaaccaaact taagaagttt agttttaagg ttaacttaaa
       61 ttatattatg cgtggaaacg ttccacgcca tctagataag ttgcttccaa tgtcatatca
      121 gggttcaata cgataaagtc tgcatcgcgt ccttgagcga tttttccaca tgtatcatcg
      181 attttacagc tgattgctgg aatcagacta gccatcatga ctgcttgttc tggtgttgct
      241 aatccccagt ctacaacatt tttaattgct tctttcaatt tcaatatact tccagctaag
      301 ttgccagatt ctagacgtgc tgttccttct tttactacta ctggaaattc gcctaaattg
      361 taatttccat caggcatccc accggccatc atacagtctg taattaatgc gacatgatca
      421 tgtcctgctt tttccattag gattcccgca gcatttggat gtacatgatg gccatcacaa
      481 atcaattcag aaaagacatg ttctaaagat aataacgcac ccaccatacc tggttcgcga
      541 tgattcaatc cacgcatacc attgaaagca tggacaaata cacttgcgcc tgcttcaaca
      601 gctttttgcg cttcttctag tgtagcatta ctgtgtccta gagaaacaac tactccttct
      661 tcagtcactt gtttgacaaa ctcttctaca ccttgacgtt ccggtgctaa agcaattttt
      721 ttgattaaac cacctgaagc ttcttgccat tcatggaaag tatccaaatc aggatccccg
      781 aaataacttg ggttttgtgc ccctttgtgt tcttctgtga agaaaggacc ttcaaaatag
      841 attcctttga ttttagctcc tggtgcttcc tgatacattt tgccgattgt ctcagctaca
      901 tcttttaaac gttctttgct tgacgtcaat gttgttggta aaaatgaagt cactccacaa
      961 gataacagac cttcagacat cactttgatg ccttctgcat cattatccat tacatcatga
     1021 ttcatatagc cgtgaatatg tgtatctacc aaaccaggag caatccattt gccgctttgg
     1081 tcaatgatgt tgacttctcc ctcaggtagt tctttggtgt attcaccaaa aagtccatcg
     1141 gtaatttcaa gatagcctgc atgtttgaca cctgacgcta aaaagaattt gtcagcatag
     1201 ataaaagttc tcattctatt ctctcccttt gtttttctac ctctaaagta ctcttattta
     1261 gagaagaagt caagaatggt ctacaccatt tacaaatttt ttgaaatttg tttcacagct
     1321 tgtgctaagc cttcatagaa tgctgctctt tctggttctt ctttacgctt gtatattaag
     1381 cttttaagca caaataaatc attgaggtaa tagtagctat tcttctcttg agcccaatag
     1441 attccttgat tcacctgttc taacgcttcg tcgatttcat tttgatggaa taagaaattt
     1501 ccataattat aaaaaatttg cgaaaattca ctttttgatc gttcatcagc tgattgatgc
     1561 agcagttgga tgcttcgact caaatagtag cgtgcttcag ccgtttttcc tgccacacca
     1621 tatatttttc cgatacaact aatcagttgt atttctgtgt cagaaacatg gattctttct
     1681 ttttggctag agtagcttaa tgcttctttc aaataataaa gggactgaga gatattttga
     1741 cgcaacgtga attcgcaaac accgagataa taataatagc gttgaaagtc agttgcttga
     1801 tacagatttt cagaaatatc agaagatttt agtacttgtt ctaacttccg ataatcacgt
     1861 ttttggaaac aactatccaa cagttcaaat aatttatcgt ttgtccgagt agtttttcga
     1921 gagagattca tcacatgatc aatcgttacc cctagccgtt gacaaatctg catcatcacc
     1981 aatacattcg gcagttcctc attattttcg atccgactta atacactttg tgaacaaata
     2041 tcatgcgaaa gcattttttg cgtcattttt tgttcttttc gaatctcttt aatgatcgtt
     2101 ccaaaggact cgattaacgt ttccataagt atcctcccca aaatatgcgt attcgcattc
     2161 tattcgtatt ttactttagg aagtcgcaaa cttcaagaaa agacttgcaa ctaaataata
     2221 tactttagtt atttaagaaa actccactag aaagagagcc tcagtgtgtt tgtctgtggc
     2281 cctcctactg aaaaatttgg tttttaaaat catttttggc gataggttgc taggaattct
     2341 ctcattttct tagctgtttg tttcaaatca tccattttag gaagataaac gattcgaaaa
     2401 tgatctggct ggttccaatt aaatccacca ccatgtacaa gtagaatatg atgttcatgt
     2461 aaaaagtcca aaacgaattt ttcatcatcc aaaatattga atcgctttgt atcaattttt
     2521 gggaatatat aaaaagcagc ttttggtttt acggctgaaa gaccaggaat atcattgatt
     2581 gcattataga tatattctct ttgttcatag atccttccgc ctggcaggag cagctcatcg
     2641 acactttgat aaccgcctag cgcggtttgg ataatttgct gagacaatac attggaacac
     2701 aaacgcatgg aggacaacat attcagccct tctatatagc ctttgacgtg tgacttatct
     2761 ccacttagga ccatccagcc tactcgaaat cctgctacac gatgagattt ggatagaccg
     2821 ttcagcgtaa caacgaacaa atcaggcgct aatgtagcaa tcggaatatg ttctagacca
     2881 tccatcacta agcgatcata gatttcgtcg gaataaataa tcaggttatt ttgtcgagct
     2941 acttcaacga tttcttctaa taattctttt ggataaagtg ccccagtagg attattagga
     3001 ttaatgataa caatagcttt tgtccgactc gttatttttg atttgatatc atcaatatca
     3061 ggataccatt ctgcctcttc atcgcaaata taatgaacag gtgtgcctcc agctaatgca
     3121 atagaagccg tccacaacgg atagtccggc attggaacca gtacttcatc tccattgtcc
     3181 agcaacccct gcatacacat cgtgatcaat tcgctcactc cgttgccggt gtagatatca
     3241 ttgatcgtca cattcggaaa atgtttcagt tggcaatatt gctcaattgc ttttctagca
     3301 gaaaagatcc ccttggaatc tgagtagcct tctgagtcac gggcattcat gatcaaatca
     3361 cgaatgactt cgtttggtgc ttcaaatcca aaaggtgcgg gatttcccgt gttcaatttt
     3421 aaaatactaa tgccctcttc ttgcatccgc tccgcttctt ctagaacggg accgcgaaca
     3481 tcgtaactga caccttctaa tttgttggat ttttcaaatt ctctcatcac ttagccaatc
     3541 cctcatttca aaaagttaga actttccaaa ttttttgtta tttactattt caatatcata
     3601 ctccatccta gaaaattttg ccacttattt aggaaaaacg taagtattaa aaaaacacaa
     3661 acaagcgttg agaacttaat tgtcatgata gtcattccat agattcgctg cccaatccat
     3721 tgcaattcca caaatgattc ctagcataaa accaagcaaa aaacgtccga atgcaagtcc
     3781 aatcaataca cctaagatca ttcccagtcc tgaaaattct ccgtcatatt tctccatctt
     3841 aataagtcct cccatttttg agcttgacaa ctatgtaaaa cctcgctatc attagtgtgt
     3901 tattattacg cagatgtgtt ggaattggca gacaagcatg attgagggtc atgtgagcgt
     3961 tagctcgtgt gggttcgagt cccatcatct gcactacaac tcgtgaagtt aattttgctt
     4021 cacgagtttt tttgtttgaa cgtttcttta tgccattata tacatatcac tgtatccttt
     4081 acaaaaagaa cgtacaatca tgtaaacagc ctgaggtgag tatatgaagg tcggaaacaa
     4141 gctactcgta gaaacttgca agtgttaaaa caattaatcg aaaaacgagt aaaatccctc
     4201 acctaatact aaagcgaggg attttttgta tattatctgt ttatcaccgt tattataaag
     4261 gtacaaatac caagcacggt taatccaaat tgcataaatt catttttttg tacactcatg
     4321 cttttcatta gctctttaac tgccattagt actaagatgg cgataacaac ataaagtata
     4381 tagtacgtcc gcattccgga agcccatact gtgggagaaa atcccataat taaccttgaa
     4441 caaaatccca ttatcatcaa aataactaaa aaaatagaag ttttcttatt gtcaaaactt
     4501 aaataaattc caacaattaa acatagtaat aatgccaaga ctactaaagt agctatccaa
     4561 gtacctggat aaaaaagtga cagtttcgtt cctaactctg taaagagatt attcaggtta
     4621 ctactactcc aaatcattgc ccttttattt ccattgacgt aagtaaagct ttgacccatt
     4681 gtattaccaa aataaataat caggtttaag aaaaacggta atgcagtcaa tatacgcaca
     4741 taatagtttt gacatttcct atatgttaga aggaaaatta agaaaaatag caataaaaat
     4801 aagatattca tgtctaaaaa aacggctttc caaatgatga aatacctaaa tctaacttat
     4861 taagaaagtt gaaattttta tattcaggaa accatttatt aatttcatgt gcagcacgta
     4921 atgcattccc tggagttgtc aaagagaaaa ttaattctgc taagcttatt attgaaaata
     4981 ctgataactt ataattatat cgtccattga caatcaaata taaactaact atacttgtta
     5041 gaacaaaaaa acaggcgttg acttgttctt gattagttaa aaatatcaat aaaggaatac
     5101 ttacccaata tatttttcta tttatttctt ctcctcttaa gagttgaaaa aacggataaa
     5161 aagttagtaa actaaatgct actggccact gatagttcaa ggtggttgct ctccaaccgg
     5221 tttcccccat tactgtcaat ggtattaaac agtatataga gcaaaccaat aataaatttt
     5281 taacatttat tttctcaaaa acat
//
LOCUS       DADVXU010000137         5009 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.143, whole genome shotgun sequence.
ACCESSION   DADVXU010000137 DADVXU010000000
VERSION     DADVXU010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.143"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..182)
                     /locus_tag="KB953_002649"
     CDS             complement(<1..182)
                     /locus_tag="KB953_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBB6867564.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="KB953_002650"
     CDS             517..951
                     /locus_tag="KB953_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBB6867565.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="KB953_002651"
     CDS             974..1294
                     /locus_tag="KB953_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867566.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="KB953_002652"
     CDS             1435..1626
                     /locus_tag="KB953_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867567.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="KB953_002653"
     CDS             complement(1697..1954)
                     /locus_tag="KB953_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HBB6867568.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="KB953_002654"
     CDS             2112..2375
                     /locus_tag="KB953_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867569.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="KB953_002655"
     CDS             2399..2587
                     /locus_tag="KB953_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867570.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="KB953_002656"
     CDS             complement(2670..2855)
                     /locus_tag="KB953_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867571.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="KB953_002657"
     CDS             3070..3441
                     /locus_tag="KB953_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBB6867572.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="KB953_002658"
     tRNA            complement(3591..3663)
                     /locus_tag="KB953_002658"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="KB953_002659"
     CDS             complement(3709..4137)
                     /locus_tag="KB953_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867573.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="KB953_002660"
     CDS             complement(4197..4955)
                     /locus_tag="KB953_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HBB6867574.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT         1744 a          784 c          978 g         1503 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt atttttatt
//
LOCUS       DADVXU010000138         4931 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.144, whole genome shotgun sequence.
ACCESSION   DADVXU010000138 DADVXU010000000
VERSION     DADVXU010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.144"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            83..970
                     /locus_tag="KB953_002661"
     CDS             83..970
                     /locus_tag="KB953_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBB6867575.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1110..2036
                     /gene="cysK"
                     /locus_tag="KB953_002662"
     CDS             1110..2036
                     /gene="cysK"
                     /locus_tag="KB953_002662"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBB6867576.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2272..2718
                     /locus_tag="KB953_002663"
     CDS             2272..2718
                     /locus_tag="KB953_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBB6867577.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2991..4343
                     /locus_tag="KB953_002664"
     CDS             2991..4343
                     /locus_tag="KB953_002664"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HBB6867578.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4514..4801
                     /locus_tag="KB953_002665"
     CDS             4514..4801
                     /locus_tag="KB953_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867579.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1693 a          813 c         1030 g         1395 t
ORIGIN      
        1 ttaaaagtat aatttaatta attaagtttg ttttaagaag agaacttttt attaagtttt
       61 tttattttga ggaggaattt gaatgcaaga gctgaagacc cgcagtgccc ggcaccatag
      121 acctaaaaga aaaatggtga tcaataaaaa agcgattgct cctttaattg gaacgagtct
      181 tttagttggg ccgatgatgg ttacagcggt accgcaacat gttcatgcag atgaacaagg
      241 atctcaaaca gtcgctgatc ctcaagcgtt tatcgatcaa atcggctggt ctgcgtcaga
      301 agttgctgca agtaatgact tgtatgcttc agtcatgatc gctcaagcgt tgttggaaag
      361 tgcatatggc acttccggat tagctagtgc gccaaattat aatctattcg gtgtgaaagg
      421 aagctataac ggacaggtcg tttatatggc aaccaaagaa tatgtaaacg gacaatgggt
      481 aacgatgaat gagccattcc gaaaatattc ttcttattgg gaatccttcc aagaccatgc
      541 gaatgttctt cgttcaacca gtttttccac gggcgattat cattatgctg gcgtgtggaa
      601 aagcaataca acaagttttt atgatgcaac agcgtacttg acaggtcgtt atgctaccga
      661 tccaaattat ggggccaaat tgaactcatt gattaatact tataatttaa cccgttttga
      721 tacgccatca acaggttcgg ctacaacaac cacaagtaca acaacaacga ctactacgac
      781 gacttcaacg tcatcatctt caacaacaga tgtaaataca aataatggtc agacatatac
      841 agtcgtgtca ggtgatacgc tatgggatat tgcacaaaaa tttggcatgt ctgttgatga
      901 tctaatggcg aaaaatggca tgtctatgtc cagttattct ttattagttg gtcaagaact
      961 aaacgtttga tttagataaa aaggcaaaga aattaccata tatggtatat ctttgtcttt
     1021 tttttgctag tcaaagacga atcactcgcc ataaatgaac agattgtatt atgataagta
     1081 agaagagcaa agacaaggag gaattgaaga tgacagctat ctataactca gtcaccgaat
     1141 tgattggcaa aacgccaatc gtcaaattaa ataaaatcgt accagaagat tcagcagatg
     1201 tttttgtaaa gcttgagttt tttaatccag gaggaagtgt aaaagatcgt attgctttaa
     1261 gtatgataga aaaagcagaa cacgatggtc tactgaaacc aggtgatact atcattgaac
     1321 ctacttctgg aaatacagga atcggattgt cgatggtagg agttgcaaaa ggatataaag
     1381 taatcatcgt gatgcctgag acgatgagta tcgaaagacg tttattgatg aaagggtacg
     1441 gcgcagagtt gattttgact cctggtgcgg atggtatttc tggatcaatc agagaagcag
     1501 aacgtttggc aaaagaaaac ggctactttt taccattaca atttgaaaat gaagcaaatc
     1561 ctcttgtgca tgagaagacg actggtcctg agatacatca agcatttgga gtaaatggct
     1621 tggacgcgtt tgttgcaggt atcggtacag gaggaacgat cactggcgct ggccgagaat
     1681 taaaacgtgt gtatccgaaa atcgaattaa tcggggtaga accagcagaa tctgctattt
     1741 tagaagggaa agaagcggga cctcacaaaa tccaaggaat cggtacaggt tttgttccta
     1801 aaacactgga tacttctgtg tatgataaag tcctttctat tagtggagac gaagcgatgg
     1861 aaacagctag agaagttggg cgaaaagaag ggattttagt aggcatctca tctggcgctg
     1921 caattgctgc cgcgctaaaa gtcgcaaaag aattaggaaa aggcaagaag gtattagctg
     1981 tggttcccga caatggtgaa cgttatttat ctacagccct ttaccaagag atataaggca
     2041 cttggttttt ctccagatag gtttatgtaa aaaactttta aagatgaaaa gaacacacaa
     2101 aaatttatga tgagaaaaat tatttgaata gagacgtaga atagtgaacg cgactgtttt
     2161 acgtcttttt tcattaaaat acaataaaat tgaattttct tttcatgtta agtggatttt
     2221 aaaatgtaaa aatagtatga taagacgaga ataacaacgt ggggtgttta catggaaaat
     2281 aagttagtcg aagaggttat cgaaaagtta aaaaaagaga acattcgaat cacacctcaa
     2341 cggtacgcgg ttttagaata tttgattgaa catcattccc atccaacggc tgatgagatt
     2401 tataaagcac ttgaacatcg gtttccaaat atgagtgttg cgactgttta caataacctt
     2461 cgcttattta cttcgattgg atttgttcaa gaaatgaagt acggcgatgc ttctagccga
     2521 tttgatttca gttcaaagcg tcattatcat gtaatttgtg aaaactgcgg taagattgtt
     2581 gattttcatt acccagggct tgaagatata gaaatggctg caagcagact gactgatttt
     2641 gagattaatg aacatcggtt ggaattgtat ggactctgtc cggattgtaa agagtcagca
     2701 aaaaaggcgc aaatgtaaaa acagtttcta caagtgttat acctaaaaaa agctagctgt
     2761 gtaaaaaaaa cgctagcttt tttatttttt tgtcttttat tcacaatctt agatataaaa
     2821 gtgaatatta ttaaaataag aatcttgtag atattgggtt cttatgcttg aaaaggaatg
     2881 aaactacaaa caaacctttg tgaattcaca agcattaaac tgttgtatcc gttttatttc
     2941 tgttatagta gttttgtcga aggacaaaat gttaatcgga gggaccataa atgaaagtag
     3001 tagtaattgg ctgtactcat gcaggtactg cagctgtaaa aagtatttta aaaaatcatc
     3061 cagaagcaga agtgacagta tatgaaagaa atgacaatat ttctttctta tcttgcggta
     3121 tcgcacttta tgtcggcgga gtagtaaagg acccagcagg cttgttctac tcaaatcctg
     3181 aagaattatc ttcattagga gcaacagtta aaatggaaca cgatgtaaca gacgtcgata
     3241 cagaaaataa aacagtaacg gcaaaagatc tgaaaacagg agaaacagaa acggtttctt
     3301 atgataaatt agtgatgaca actggttcat ggcctattat cccaccgatt agtggaatcg
     3361 atgcgaaaaa tgtactccta tgcaaaaact acaatcaagc aaatgaaatt attgcacaag
     3421 cgaaagaagc aaaacgtgta gtaattgttg gtggtggata tatcggtatt gaattagtag
     3481 aagcttttgt cgaatctgga aaacaagtga cattgattga tggattagat cgcatcttga
     3541 ataaatatct agataaacca ttcacagata tcttagaaaa agaattgaca gaccgcggtg
     3601 ttactcttgc gctaggcgaa aatgttactg aatttatcac agatgaagaa ggaaaagtga
     3661 aacaagtagc tacaccaaca gatacatttg atacagatat ggtcatttta tgtgtgggct
     3721 tccgtccaaa tacaaaactg atcgaaggaa aagtagatac actgccaaac ggggcaatca
     3781 aagtgaatga atacatgcag acaagtaatc cagacatttt tgcagctgga gactctgcag
     3841 tagtcaacta caatccaagt ggcacgcaaa actatattcc tttggcaaca aatgccgttc
     3901 gtcaaggttt gcttgtggga aataacttga cagatcataa aatggcgtat cgtggtactc
     3961 aaggaacttc aggactttac ctatttggct ggacgatcgg ttcgacaggt gtgaccaaag
     4021 aaagcgcaac tttgaacgat ctagaagtcc aagcgactgt atttgaagat aattatcgtc
     4081 cagagttcat gccaactact gaaaaagtaa tgatggagct agtctatgaa aaaggaacga
     4141 atcgtatcgt aggtgctcag ttcatgtcca aatacgacat tacacaatca gcaaatacca
     4201 tgtctttagc tgttcaaaat aaaatgactg tagaagatct cgcactttca gatttcttct
     4261 tccaaccaca ttttgaccgc ccatggaatt acttgaacct acttgcacaa gcagcgctag
     4321 gcgaaatcga aactgcacaa taaagaaaaa gggttgtgac acaattttgt cacgaccctc
     4381 tgtctttttt tgtgaaaagt acaattagtt gtctttcttc gaattttttg tcacaataag
     4441 cagcaaagat aaagctgtta cataaaagaa caagcagctt tatttgattt tgaaaagaaa
     4501 ggtgagaaag aagatgaata aagaatattt tgtccaacaa gatgcatttt tagaagatta
     4561 taaaagtgct tcgaagaaat taacgatcaa tattgatgag ttggcagatc aagtactctc
     4621 tcagttgaat gaaacgcaag aggaatatta cacgatgcca gcaaatgacg ccaaagataa
     4681 tgtttctcat cgttttcctt tcagtagacg catctactca gatgaaaatg acggcacatt
     4741 gaatacagag tacacatatg aaggtcagcc atatgtgcta gaaactgatg atgatcttta
     4801 aagacagttt ttgaaaaggc cgctcatcct taagtgatga caaaaaatca tcacaattac
     4861 cgatgactgt ctctttacta tcttttaatc agaaaaaggg gttatgacaa gctttgtcaa
     4921 cccctttttt t
//
LOCUS       DADVXU010000139         4903 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.145, whole genome shotgun sequence.
ACCESSION   DADVXU010000139 DADVXU010000000
VERSION     DADVXU010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.145"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(82..1890)
                     /gene="pepF"
                     /locus_tag="KB953_002666"
     CDS             complement(82..1890)
                     /gene="pepF"
                     /locus_tag="KB953_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBB6867580.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2066..3064)
                     /locus_tag="KB953_002667"
     CDS             complement(2066..3064)
                     /locus_tag="KB953_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HBB6867581.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3290..3943)
                     /locus_tag="KB953_002668"
     CDS             complement(3290..3943)
                     /locus_tag="KB953_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HBB6867582.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4275..4673)
                     /gene="spxA"
                     /locus_tag="KB953_002669"
     CDS             complement(4275..4673)
                     /gene="spxA"
                     /locus_tag="KB953_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HBB6867583.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1450 a         1021 c          753 g         1679 t
ORIGIN      
        1 taaaaaaata cgtcgactcc cttacaagag agccgacgta tttttttcgt ttctattcga
       61 gaaaatcaaa aatagaaagc cttataaacg atcgatcaat tcttctaatt catttagacg
      121 ttgttcaaac atcgacattg catcttcaat atatgcagcc tgtgtcatat caactcctgc
      181 ttttttcatt acttccacag gataatcgct gttacctgct tttaggtaag ccaagtagtt
      241 ctctagtgcc tctggttcct ggtttagtat tttcttcgcc agtgctgaag cagcagaaaa
      301 gccagtcgag tattggaaaa catagtaatt ataataaaaa tgcggtatcc gtgaccattc
      361 gaatttaatt tctgggtctt cttcgactgc tggaccataa tattttgcat tcaatttacc
      421 ataactatca cttaggtatt cactagtcaa tggcacacct ttttcatctt ccgtatgcat
      481 gaaatgctcg aattcagcga attgtgtctg gcggaaaact gttcctttaa acccatccaa
      541 gtaatggttg agtacataag cccgtacacg agggtctttt tctgtttcca ataaatactc
      601 cgttaggata ttttcattcg ttgtcgaagc aatttctgct aaaaagatgg agtagtcgcc
      661 gtacacataa ggctggttcg aacgagtgaa ataactatga acactatgtc ccatttcgtg
      721 gacaagcgta aatagctgat ccagcgtatc atgccaattc aataaaatat atggatttgt
      781 gtcatagctt cccgaagaat aagcaccgct tcgtttccct ttattttcga caacatcgat
      841 ccaacgttca gagaatgctt tttctacgat ggccatgtat tcttcaccca ttggtttcag
      901 tgcttctaaa gctttttctt ttgcttcttc gtacgtaaag gtaattggag cttcccctaa
      961 gaccggtgta taaagatcat acatgtgcag tttttccact tctaataaac gtttccgtaa
     1021 ttccatgtat cgatgtaaca aaggcaaatg cttgtttacc acgtctacca aagtatcgta
     1081 tacactttca ggaatatgat tattgctcaa agacgcttct ctggcagagc tgtaattacg
     1141 gactttcgct ttaaaattat gtccttttat atgtgtgcct aaagtagaag caaatgtatt
     1201 tctaaattgt tcgtaaacac tgtacaaccc cttaaatgct gcttcgcgca cccttcgatc
     1261 cgtgctttct agcaactgac catacacgcc atgagataat tggactattt caccattttc
     1321 tccttcaatc gttggaaaaa ctagatctgc attattcaaa acagcaaatg tatcacttga
     1381 agcatcaaag atttcacctg ctccagcaag aagagattcc tgctcagcag ataagacatg
     1441 ggctcgatta tctactattt gctggatata atggcgatag acttccaatt ttggttcttc
     1501 tttgaaatat tgccaaatct ggtcatctga caattgcaat atttctggtt caaaccagga
     1561 aacggcttca ctaactttcg aaaacagact gcttgctctt gcataaagcg cttggtaatc
     1621 tgtatttcca gtgtcttgat cgtttttaag gtgcgcatat acataaatga cttcagtttg
     1681 gcgataaacc ctcaatacga attcaatcgc atttaaaaat tgagaagcgc cttgatcaag
     1741 tgttcctttg tgtttttcag attgttttaa ctcttctgat aattccaaat atttctcatc
     1801 aaactcttgg tcgcttgaaa agatcttggt caagtcccaa gttaaatttt caggcaattc
     1861 ttctcgtttt ggcaactgct ttacttccat gaaatccact ccttttcttc ccttagctct
     1921 atctatcaaa atagatcata gtctaacatc cacatgtcac agcatctaac ttttctactc
     1981 attcatgtgt ctgcttccat caattttagc ataactaaat cttgttttac atagaaaacc
     2041 gattcctagc ctatttttat acttactatt ttttcaaaga agacaagtgt atgcattcgt
     2101 taaaaaactg ctgatagacc atctcttcat caatcatagg aaaaagccat tcccttttat
     2161 gttcttttat ttggcaaagc catgagttga aatcaggaac tttctggttt tccaaggctt
     2221 cttcgtacaa cattcgataa agaaaaacct gttcttgtaa ataaaagaaa aaccgacttt
     2281 tttgatagat ccacgatgat agatgcaaaa gatgtttatg acgcaagtag cattgttctt
     2341 gtatggatac tgtattcttt tgcttgtgaa taagttggcg tgtcagccat ttcttcccat
     2401 cttcactcac tttttctatt tttttcattt ggttcatttt aatttcgtta ctagtgaata
     2461 tttctttaag gtccctacta gaaaaagtcc agcagctact gcaaaaggaa atatgccctg
     2521 atacagtctc tgtaatatga tgatacaaat aaagcttttg tttcttgaga tcagctttcc
     2581 agcaatgcac tcctcttttt ctattatagt agcagcaact tttttctatc aatgagaatt
     2641 gcgattgtcc aagaaaagaa ttaccaagta tccaccatac ttggtaactg ttattcaaat
     2701 aattttgggt tctttcaata aatctctggt gagacagtct agaacattgg atttctattg
     2761 ctagattcgc caacaaaata tccggccttt gccgcaacct cggcaaatag gcttccaaaa
     2821 atactggttc tactgactgt cccagccaat ccatgaagca ttcctttaac tgtaagtgct
     2881 cttttgattc tctttcacta aagaccgaac agttatctga tttttgatgg gcaaaatgag
     2941 cacatttttt ctttcccttt ttcaaaatca ctctttttcg acagccacga caataatatt
     3001 tctttccttt ttctgcatgt ttagcaaaac agcacttatt atattcattc attgcaagca
     3061 gcatttttct ccctctgaac ttatttacgt ctaagaattt atttacacga aaaaagaggg
     3121 cgtgaaaaag ctattctgca tcaagtaata agaacagcta aacaaaaata gttcccaata
     3181 tttttcgttt ggttgggctt gttaccgcag atgcaagctt ttgaacacat ttatacgaaa
     3241 aaaagactgc gacacaattt ttgtcacagt ctcttactat aatgctgatt tatacaaaat
     3301 aaaatttcgt tcgctcgata gcattacgcg ccatcagaca ttgtccatat tcattcaatg
     3361 tttctggtgt acgatcgctc ttttcagcaa actccaaaat tcgagcaatc tgattctcta
     3421 catccgcttg gttgagattt tccatccaga agtgaacgac taggtaatag cgctcttcga
     3481 ataaatacaa atcagtccaa gcagactcta gttcgacatc tgttgcaagt tggatcactt
     3541 gttcaaaatc ttcaaaagca aaagttgctt gtctttcctg ttcttctagg gcgcttccag
     3601 ataattcttc ctccggatcg ctgccaataa tctgctgacg aagaaagtca ctcacatctt
     3661 ctgaatcaac agtagaatct tctgattgag aaatttcttc atccggaaga tttttactga
     3721 taaacagttc taatccgtct ccttttggca aaacttggaa agtgacagct tcgcttcctc
     3781 ggaactcctc ttcgatatca acttcttcca aaatactata aaagaaattt tcgatttctt
     3841 tgtgattgcc aagtaaatcc aaaaatgaaa cgccccgttc ctctaaatct tcatgaccaa
     3901 ttagtacacg gatcgtattt tcattaatat gttccatttc cattttgcta cacctcactt
     3961 tgccacaatg tttaatatag attcattgta gcgaaatcag cttaaatgta aagaaaaaaa
     4021 atctcgctgt taagcgaatt taataaaacc agcggatttt tcaacctttc aagctatctt
     4081 atttaactat aacatttttg gcagacgaat actatatagc cgacttttca gctaattttc
     4141 cctctctatt tttcgtgcaa tcatcatctt ctactacctt tttatacatc taatcaagaa
     4201 tactttttgt aaaaacaaaa aaacttggaa aaactatctt tgtaatagtc ttttccaagt
     4261 ttcgtattat ccttctataa tccagccatc aattgtgctt gacgtaattc taattgtcgt
     4321 acatcgcgcg gtaggaaccg acgaatttca tcttcattaa aaccaacttg cagacgttta
     4381 tcatcaatca tgattggacg gcgaagcaat ccaggattgt tttgcacaag ttccaataat
     4441 tcttgtaacg gcagatcatc taaatccatg ttcagttttt ggaatacttt ggaacgtgtc
     4501 gagatgattt cttctgttcc gtcttctgtc atttgtaaaa tcgcttttaa ttcagaactg
     4561 ttcaatggtt ctgaaaaaat attacgttca acaaatggaa tctgatgttc ttgcaaccac
     4621 gcacgagcct ttctgcagga agtacaactt ggggaagtat ataatgttaa caacgtgcac
     4681 cactcctttt cgtgataaga atcacttttt tgtaacagaa gatcataata atctcccatc
     4741 tgtttctatt atacataatc aacttttaaa tagctacctt tttttcataa tttttttaaa
     4801 aaaatgaaag ttatctaagg ttaatataca gtaaaaccag cattctcgct ttttttatca
     4861 tttaaaaatt agagttttca aatctaattc aaattttcta aac
//
LOCUS       DADVXU010000140         4759 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.146, whole genome shotgun sequence.
ACCESSION   DADVXU010000140 DADVXU010000000
VERSION     DADVXU010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.146"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            174..518
                     /locus_tag="KB953_002670"
     CDS             174..518
                     /locus_tag="KB953_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBB6867584.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            519..938
                     /locus_tag="KB953_002671"
     CDS             519..938
                     /locus_tag="KB953_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBB6867585.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1030..1380)
                     /locus_tag="KB953_002672"
     CDS             complement(1030..1380)
                     /locus_tag="KB953_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867586.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1346..2677)
                     /locus_tag="KB953_002673"
     CDS             complement(1346..2677)
                     /locus_tag="KB953_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HBB6867587.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2933..3499
                     /locus_tag="KB953_002674"
     CDS             2933..3499
                     /locus_tag="KB953_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBB6867588.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3644..3731
                     /locus_tag="KB953_002675"
     tRNA            3644..3731
                     /locus_tag="KB953_002675"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3676..3678,aa:Ser,seq:cga)
     gene            4065..4631
                     /locus_tag="KB953_002676"
                     /pseudo
     CDS             4065..4631
                     /locus_tag="KB953_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
BASE COUNT         1739 a          797 c          910 g         1313 t
ORIGIN      
        1 aaataaaaaa agcgttagca actatgctta aaaagcatat cgactgaata aactaagcat
       61 tggacaggct gaaagactac atttaaaatg aaatgcgaag aaacaataga aaataaagtt
      121 gccttagaac catagttgaa tcacctaaca gtcgtgaagg gagaataaga gaaatggcag
      181 aaaaacaaac cgcaggacga gatcaattag gagattttgc accaaaattt gcagaattga
      241 atgatgatgt tttatttgga caagtatggt ccagagaaga attatctgct catcagcgta
      301 gtatcatcac catatctgca ttaatttcag gaggaaattt tgaacaattg ccagctcatc
      361 tgaagattgg caagcaaaat ggcattacta gtgaagaaat agcagaaatc attacccatt
      421 tagcttttta tgtaggatgg ccaaaagctt ggtcagcttt tagtttagca aaagaaatct
      481 atttagaaga aaatagcgaa gataagaaag aggcgtaaat gacgacaaaa cacgaagaaa
      541 tacacaatgg cgtgattttt ccagcaggtg aaaaaaacga agcttacgct caagtattta
      601 ccgggcaaag ctatctgaat atgttagtga atgatcccga agtttctgtt ggtgtaggta
      661 atgtaatttt tgaaccaggt tgtcgcaatc actggcatgt ccataaagac ggctatcaaa
      721 tcttacttgt gaccggagga gaaggctggt atcaggaaga aggaaagcca gcaagacgat
      781 taatggctgg agatgtcgtc gtaacgcaca aagggattaa acattggcat ggtgcgacaa
      841 aagacagttg gtttgaacat ttggcgatca ctgctggtaa ttcagagttt ttagaacctg
      901 taacagatga agtgtatgaa ggattggaaa atcaataatg tcaagcttga acagctcctg
      961 aaagtagatt ttaatcgaac tttcaggagc tgcttttttg attcagtctt ttataaatag
     1021 aaaatagggt taataatagg aatgagaagc acgtcgaaag atcttaatat tgataatcgg
     1081 ctgggcatac atgatttcca cgtcatcaat caaactacct aacagatgat aatcttcccg
     1141 atcatgtcgg ggattaccta atagttcttg atagtagatt tccatactag ggtcccgagg
     1201 attgttcagc aaatcagcaa gtacatctaa atcttctggc tgagtttctg ccgtaccctg
     1261 tatctctatg aaaagccctt cctcagcaaa ttctactcca atacgtattt cagttatttc
     1321 atgggaatat aaataagtaa gaagctcatc gatgattctt tttgataatt ctttttccaa
     1381 gtgtcttttt ttcctttctg acagcggtaa agttatctag tatcggtaca aggatacctg
     1441 caacgaaacc agttgaaaaa ccactgttat atagatttag tcctccgtgc aaagtgctga
     1501 cgttatggac aagagcaata tgtagtacac cagctattac accgtaaaaa ctgccgtaaa
     1561 aaccgcttat gggcgcgaga cctgtcccaa acacagctgc aacaagcatg ccagtactgg
     1621 tgatctcatg aaaagttcca aacaaagaag caataaatat tccgactagt atcggaaaac
     1681 tgttttttaa atgacaacca aaagcactga aacccaccgc agataaaata gcaccgatga
     1741 caggtccatt taactgaccg ccaaccagca aaacataaga agacagcatc agaccaacta
     1801 agcctatgtt gatcaaagta gcaccaatac cagaattagc aatgaaatca gttgtcaatt
     1861 ttccagatga atcgaaaatt tctcgaatcc ccaaaagccg aaaggaatta taatagaaac
     1921 caattccgac tgtcagcgaa aaaaagccaa aaataatcca aatgagagga aagtgatatt
     1981 cattgaaaac caatgtattt tcgacgattg aatagctgaa taacctcaaa acagcagtaa
     2041 aagtcatcgc aatcaaccca gaggtgaatc caatattata aagtgtgaat ccacgatgga
     2101 aaactaaagt ttgagcagct aagggcggaa gaagtagacc aattgctatg ccagctaaat
     2161 tccccaataa aatacctatc agtaaaggaa aacgcatacc aaaagtgata taactaacga
     2221 ccggtgataa tgcgctacca aacagtccga tcatgatgta ctgagaaaaa ggtttattga
     2281 caaatacagc gtatagatac acacctaaaa tgattgagat agaattataa aggtttttgc
     2341 caaaaaaaga gaaaccggta actgttagaa tcgaagcaaa taagggaccg tttaaaatca
     2401 ccttgtgccg ataagctaaa agcacagaaa gtaaggtcaa aatccctaca tttatgaatg
     2461 cgcttccgaa accgcctaaa gcaaaataat ctgtcaaaag attgcttggc gaggtaagaa
     2521 tagttagcaa tccactccaa ttatcagtag gagtattaga aagcaagcca ataaaaagaa
     2581 aaaaaacacc tgtaaaacca agataaaaat actttctttt ctgtgaaacg attcgttttt
     2641 gaggatgaat ataaataacg tcagtttcgt tttccattac acaccttctt tttcattttc
     2701 tttaactata acttgaaatg aaagatttgg acaactatca tttaggtcaa aactaaaaat
     2761 gtaagggaac ccattgaatt taaccaaatt tttttaattt tgcaaaaaaa agttaaagaa
     2821 gactgaaaaa aaatttaaaa tataacgttt tgagtaaaat gtttgcaaaa aatgctaaaa
     2881 atagggtaca ataaaacaac atatactgca gtaaagaagt gggggagttt taatgagaag
     2941 aaaagtctat accaaggatc aaattctaaa agctgcttat gatgttgttg caaaggaagg
     3001 gtttaaagga tttactgcga gaaatattgc taaaaagatg gggatttcga cacagccgat
     3061 ttatctggaa ttcaaaaaca tggaagattt aaaaaatacg ttgctggatt ctatctttga
     3121 acaactaaaa gaagatgttt tccctattga acgtactggt gataaaatca ttgatctcgg
     3181 tttgaactat atctactttg caaaaaagca tcatagtctt tacatggcat tgtacattga
     3241 tgaacatggg gacggaaaga aaatgcatga tttttcatac aattatttcc gtgaaatagt
     3301 taaagaagat gaaagataca atcaattatc ggatgaatat gtcgatgctc tgcatactgg
     3361 tacatgggta gcagtgactg gcttagcgtc attgatgtca tcaaacatca tgcatccaac
     3421 agaagaacaa atcattgcac tgatcaaacg gacaattgac tcgattttag cattagataa
     3481 tcctgaaata atcagatagt gttctatcag tatgaatttg atcggtaagt ccgttggctg
     3541 caaactgtca atggactttt ttgctaaaaa aagcgatttc tgttatttat ctattagaaa
     3601 taggcttgac atttcgtgaa aaaagtttaa aaatagtact tacggaaggt tggcagagtg
     3661 gtaatgcacc ggactcgaaa tccggcgaac cggctaatac cggcgcgcag gttcaaatcc
     3721 tgtaccttcc ttatatcaac gattagaggt tgtgaagaag ctgtcctgca tcaagtaata
     3781 agaacggcca aacaaaaata gcttttccta ttttttcgtt tgattgggct tattaccgca
     3841 gatgcaagct tttgaacacc gtttgttcgg aataaggggc tgtgacaaga cttttgtcac
     3901 agccccttat tgttgtactt ttaacctaca atattttttg attccccctg agtactattt
     3961 tttataaatg ctagtaaatc aatcttcttt ggcctttttt tgataaaaaa aatactagat
     4021 gtaagataaa agtgtaaaaa cgatttgttt taggagggga aacgatgtgt accgcaatca
     4081 cttatgcaac gaaagaccat tattttggaa ggaattttga ttatgagatg tcttataatg
     4141 aagtggtcac tattactcca agaaattatc gtttcgattt tagaaaggta aaaaatttag
     4201 ataaacatta tgcaatgata ggaatagcag ctggggtatc caactatcca ctctattatg
     4261 aggctacaaa tgaaaaaggg ttgagtatgg caggactgaa ttttcctggc aatgctgatt
     4321 ataaagaatt acaagaagga aaagataacg ttgcaccatt tgagtttatt ccatggatac
     4381 tagggcaatg ctcaaccata gacgaagcta aagaattatt ggctactatc aatctagtca
     4441 atatcgattt tagtgaaaaa ctacccttgt cacctttaca ctggttatta gctgataaag
     4501 aaaaatcgat agtaattgaa agtatgaaag atggacttca tttatatgat aatcctgtcg
     4561 gtgtgttgac aaataatccg ccatttgatt atcaattatt taatctgaac aattatcgct
     4621 cgctatcaaa tggaacgaga gtataaaata ttttgtgtaa atgaaaaaat ccatacaaaa
     4681 aaggaagtcc cttctgtaga ataaagttaa cgacaaccaa ttcacagaaa agaggacttc
     4741 cctatgaatg attttacta
//
LOCUS       DADVXU010000141         4694 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.148, whole genome shotgun sequence.
ACCESSION   DADVXU010000141 DADVXU010000000
VERSION     DADVXU010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.148"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(52..522)
                     /locus_tag="KB953_002677"
     CDS             complement(52..522)
                     /locus_tag="KB953_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HBB6867589.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(681..1139)
                     /locus_tag="KB953_002678"
     CDS             complement(681..1139)
                     /locus_tag="KB953_002678"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBB6867590.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1310..2455)
                     /locus_tag="KB953_002679"
     CDS             complement(1310..2455)
                     /locus_tag="KB953_002679"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HBB6867591.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2468..3385)
                     /locus_tag="KB953_002680"
     CDS             complement(2468..3385)
                     /locus_tag="KB953_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HBB6867592.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3813..4376
                     /gene="efp"
                     /locus_tag="KB953_002681"
     CDS             3813..4376
                     /gene="efp"
                     /locus_tag="KB953_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBB6867593.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4477..4647
                     /locus_tag="KB953_002682"
     CDS             4477..4647
                     /locus_tag="KB953_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867594.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
BASE COUNT         1471 a          851 c          883 g         1489 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tcttttttta tttttattaa gttaaagaac
       61 ggattcaatt gcagcggtca ttttcaatgg agaagtttgt ggtgcaaatc gttcgattac
      121 tttgccattt ctatcaatca aaaatttagt aaaattccat tttattgctt tagtgacagt
      181 accaggagct tcatttttaa gataagtaaa aagaggatct gtattttctc catttacgtc
      241 aatcaattga tgcataggga aggtaacgcc ataagtggtt ttacaagctt ctgctgcttc
      301 agtcgctgaa tctagctctt gtttaaattg attggaagga aagcctaaga taaccagacc
      361 ttgtgtctga taagcttggt aaagttcttc taattgttta aattgaggtg ctagaccgca
      421 ttttgttgca gtgttaacaa tgattatcgg tttgcctgca tattttttca atgaatagac
      481 agtgccgtct tctaaagtag tttcaaaatt ataaatagac ataaagtttt ctcctttcta
      541 ttttatataa taaaaagagc gctaaactga aaactagcta tttgttttcc cgtttagaac
      601 tcttttcaga caataaatat ttaataaaat acgaagataa gatagctata ttgcttctca
      661 tctttacgtt cttttagaaa ctatgcttct acttcagacc attcatcgcg ttttgctaaa
      721 aattcacgag ccaaagcttg tgcaccttct agtgtatggc tggcagccca gccacattgt
      781 gtttcattgc ttgcaggtac ttcagtagct tcaagaacat ctttcattgt tgcttcaaga
      841 acatctaaaa tgtcttcgta gctttcatga ttcaatacag tcaagtaaaa gcctgtttgg
      901 catcccattg gagaccagtc tacgatgtaa tctgcgtgat tgcggattag ttcggcagtc
      961 aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt ctttatttgg ttgtttaaag
     1021 cggacgtcgt atttaatgat gacatcgcca tgcgcacccg tttttcgatc agctacacgt
     1081 acataaggtg ctttgacttt tgtatggtca agattaaaac tttcgacatt cattctcatt
     1141 tgtaagactc ctatctgttt gttcattcgt tttgatgctt ttcactaaag aattatttag
     1201 tgaaatttac aaaaacgtct ttctacttga ttctaacact tatcgtttcc aaggtgtagc
     1261 aatctgaaat attttttaga tttttctgct aagtcgttta ttgggacttc tagttcttga
     1321 ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg accatactca attcctacag
     1381 aaagacggat caagccgtcc tttatgccag cctcttctcg tttttctttt ggaatagacg
     1441 catgagtcat gacagaagga atttccacta aactttctac gccacctaaa ctttctgcta
     1501 aagtaaatag ttggagtgat tcaacaaatg gaatagcaga ttcttcattt tttaaagtaa
     1561 aagaaatcat tccgctaaag ccgctcatct gctgcttagc aactccatgg agttcatgat
     1621 cgggtaatcc tggatagtag accttttcta ctgctggatg agaaaataga aaatcagcta
     1681 ccacaaaagc atttttttga tgttcctcca tacgaacact tagtgtcttg attccgcgct
     1741 gaagtaacca gctatcttgg ggaccaagta cggctccgat agcattttgg taaaatccga
     1801 tctgatcggc tagatcttta tggttagaag tcactaaacc agctactaca tcgctgtgtc
     1861 cgcctagata tttggtaccg ctatggacaa cgatatcaga accgagatct aaaggtcttt
     1921 gaaaataagg agtagcaaat gtattgtcag caataactat caatccatgt tgatgagcca
     1981 gagtggcact tttttctaaa tcagtaatct ttagaagcgg attgctaggt gtttcaagat
     2041 aaagtgcttt tgtattgggt ttgatggatt gttctatttt atcaagatta ctcgtatcta
     2101 tgatcgtgta ctcgagacca tttttagtaa gaactttgtc aaatagacgg aatgttcctc
     2161 catagacatc atctcctaat aagatatgat cgccagcttg aaaaagagaa aaaacggcat
     2221 gaatcccaga aagaccagaa gaaaaagcga atcctcgaac accgccttcc aaatcagcaa
     2281 tcagttcttc caatgcaaaa cgagtaggat taccagatct tgaatattca tattgtttag
     2341 gctggccgac gccatcttgc cgataagtgg atgtttgata aataggtaca ctgacagctc
     2401 ctgttgtggg atcttcactg atgcctccgt ggatcaattt tgtttgaatg tgcatgtttc
     2461 tttcctccta taagtaaatg ttttttgata aatagcggtc tgctgcgtct ggaaaaatcg
     2521 taacgacacg atgaccaggt ggtaaacgac gaatttctct taatgctgct gcaaaagccg
     2581 caccactcga acttccaact aatagtcctt gttctcgtgc taactgccta gtatagttaa
     2641 acccttctac atcagagatt gtctcgatct gattgattga aaggggagaa agaaacggcg
     2701 gaatgaattc tacaccgata ccctcaattt cgtgaggggc tggatcacca ccatttaaca
     2761 cagagccctc tggttctaca ccgattattc gaatgtcggg atatttttct ttcaaaaagg
     2821 tagaagttcc agcaaaggtt ccaccactcc caattcctgc tacaaaacta tggatatttt
     2881 ctttgatttc atgaaagatt tctggcccta acgtatgata gtaagcagca ggattatctt
     2941 tattttcaaa ttgaagaggc agatagctgt ttgatatctc ctctgcaagt tttttacttt
     3001 tgctgatggc accgatgatt ccttgctcac taggagaatg gatgatcttt gctcccaatg
     3061 ctttcatcaa ttgttgtttt tctaagctaa acttctctgg aacaacaaaa accgttctta
     3121 atttgtatgt gagtgcggcc agagcgatcc caattcctgt atttccggca gttggttcga
     3181 tgatcgtcgt tttttcattg atttttcctt gggcaaatcc ttctttgatc aaataactgc
     3241 ccaatctatc tttgatactt cctccaggat ttaaatattc tagttttgca taaatagcag
     3301 aacctttagg catagaatag tgctgattgt caaagcgata aagcggtgtg gaaccaatag
     3361 cttctgtaat tgtcgagata atcatgtggg cctcctaaaa aaagataata aaaaaaggag
     3421 ctgactatta aatcagctcc ttgaaagaca tagagaaaaa ttgagtagat aaaaagataa
     3481 gttacctggt actcattcta tgaagggcaa ttaatagaca tgacaaatag tatgctgctt
     3541 ttatgcacca tacaacagca acgattcaat tgtttttggt tgctgactgt catgtgtatc
     3601 cctccaagta aaatttttat taacgatagc atgaaaaaaa ataaaagtca aaggcttgaa
     3661 agaaaaaaat atttcgatag catcgtttac ccgtgtttat ttttctgaca aataggcatg
     3721 agaccttaaa agatagagaa tactctagtt ttctggccgg ttgtctgata caataaagtt
     3781 gattatgata aagaaaagaa ggagcaaaaa taatgatctc agcaagtgat ttaagagccg
     3841 gtatgacttt cgtacaagac ggcaaactga ttaaagttct ggatgcaagc caccataaac
     3901 ctggtaaagg aaatacagtt atgcgtatga aactgaaaga tgtccgttca ggtgcaacat
     3961 acgacacaac gttcagacca gaagaaaaat ttgaaaaagc acatatcgac acaaaacctg
     4021 tacaatattt gtacacgatg gatgatacag ctttcttcat ggatttggaa acatatgagc
     4081 aatatgaaat tcctgtagaa acagtagaag aagaaatgaa atatatccta gaaaacatgg
     4141 aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt tcaattacca acaactgtcg
     4201 tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc aacagtcact ggttcaggta
     4261 agccagcgac aatggaaact ggcttagtag tcaatgttcc tgacttcatc gaaacagatg
     4321 aattgttgga aatcaataca gcagaaggtt cttatgtaaa acgtgcgtca aaataattcg
     4381 ctaacggatt gataagaatt tgaaaacaga gcttgacctc ttgaaaggca gatcatcgga
     4441 tctgcctttc aagaggtttt ttgtggaatc agcaaattga aaaataaaaa agtatttgat
     4501 tttatttata ttttgaaaga tgaatggcaa aagctagaga aagaaaaaac tgcacttatt
     4561 tatgaagaaa ttgaaatgat tcataaaatt gaaaatatcc gaactattcc gaactatatt
     4621 gaagcgaaaa tattttttca tcactaaata gtgttgaatt tatgttttta tgtataaata
     4681 gcttctaata gttc
//
LOCUS       DADVXU010000142         4620 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.149, whole genome shotgun sequence.
ACCESSION   DADVXU010000142 DADVXU010000000
VERSION     DADVXU010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.149"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            153..959
                     /locus_tag="KB953_002683"
     CDS             153..959
                     /locus_tag="KB953_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HBB6867595.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            977..1684
                     /locus_tag="KB953_002684"
     CDS             977..1684
                     /locus_tag="KB953_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HBB6867596.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWLFLHTP
                     LTIIQRRGHEVKEFPEPNSDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKII
                     GQVFADKEPQALYKITNGSIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKC
                     KICYPLPESVDIQEENQ"
     gene            1681..2652
                     /locus_tag="KB953_002685"
     CDS             1681..2652
                     /locus_tag="KB953_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HBB6867597.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            2645..3673
                     /gene="vanB"
                     /locus_tag="KB953_002686"
     CDS             2645..3673
                     /gene="vanB"
                     /locus_tag="KB953_002686"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HBB6867598.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            3679..4287
                     /locus_tag="KB953_002687"
     CDS             3679..4287
                     /locus_tag="KB953_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HBB6867599.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(4353..4526)
                     /locus_tag="KB953_002688"
     CDS             complement(4353..4526)
                     /locus_tag="KB953_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867600.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         1300 a          972 c         1230 g         1118 t
ORIGIN      
        1 attttgaccg acaggtataa ccctgccggt ctttttttgc tgctacagga aaactacaga
       61 ctggctacag ggaaagtaca gatgcgcttg ccataataac aatcgtacca gccacaaatc
      121 gtagttttat tgcaaaggag gcattcgatc aaatggaaaa aagcaactat cattccaatg
      181 cggatcatca caaacggcat atgaaacaat ccgtggaaaa gcgggctttt ctatgtgcgt
      241 tcattatttc gttcacagtc tgcacgctgt ttttggggtg gagactggct tccgtattgg
      301 aggcaacaca gataccgccc atccctgcaa ctcatacagg cagcagcact gacgtagtgg
      361 agaatttgga ggaaaacgct cttgccaccg ccaaagaaca gggagatgaa caggaatgga
      421 gcctgatttt agtgaacagg cagaacccca tccccgcaca gtacgatgtg gagcttgagc
      481 aactatcaaa tggtgagcgg atagatattc ggatttctcc ctatcttcaa gatttgtttg
      541 atgccgcaag aactgatgga gtttacccga ttgtcgcatc cggataccga acaacagaaa
      601 aacagcaaga aattatggat gaaaaaattg ccgaatataa ggcgaaaggc tacacctctg
      661 cacaggctaa agcggaagca gaaacttggg tggccgtgcc gggaacgagc gagcatcagc
      721 ttggtcttgc tgtggatatc aatgcggacg gaattcattc aacaggcaac gaggtttata
      781 gatggctgga tgaaaacagc tatcgttttg gttttattcg tcgctaccca ccagacaaga
      841 cagagataac cggtgtgagc aacgagccgt ggcattaccg atatgttggc atcgaagctg
      901 ccacagagat gtacaaccaa ggggtttgcc ttgaggaata tttaaaacca gaaaaatgag
      961 aaaaggatgt tatgttatgg acagaaaaag attgacacag cgtttcccgt tcctgcttcc
     1021 aatgagacgg gcgcagagaa aaatgtgctt ttatgcggga atgagatttg acggctgtcg
     1081 ctatgcgcag acgatagggg aaaaatcgct ttcccatttg ctctttgaaa cggattgcgc
     1141 attatacaac cacaataccg gatttgacat gatataccag gaaaacaagg tgttcaactt
     1201 aaagctggca gcaaagacct taaacggctt attgataaga ccgggggaaa ccttttcttt
     1261 ctggttgttc ctgcatacgc cattgacaat tatccagcgc agaggtcacg aagtaaagga
     1321 gtttccagag ccaaacagtg acgagattaa aggggtggat gcaaccattt cagagggctg
     1381 gattgattta aaagtgcgaa acgataccga ctgcacctac caaatatggg tgaccttaga
     1441 tgatgagaaa atcatcggtc aggtgttcgc cgacaaagag ccccaagcat tatacaaaat
     1501 tacaaatggc agtatccagt atgtccgtga aagtggcggg atttatgaat atgcccaggt
     1561 taaacggatg caagttgcct taggtaccgg ggaaataata gattgcaagc tgctttatac
     1621 aaacaaatgc aaaatctgtt atcccctccc ggaaagtgtg gatattcagg aggagaacca
     1681 atgagaaaaa gtgtgggcat tactgttttt ggatgcgaac aggatgaggc aaatgctttc
     1741 cgcgctttat cgccggattt tcatattatc cctacgctga ttagcgatgc gatatcggca
     1801 gacaacgcaa aattggccgc tggcaatcaa tgcgttagcg taagccataa gtccgaggtt
     1861 tccgaggcga caattcttgc gctgagaaag gtcggggtaa aatacatttc tacccgcagc
     1921 atcggctgcg atcacattga tacgactgcc gccgagagaa tgggaatctc ggttggcacg
     1981 gttgcgtatt cgccggacag cgttgcggat tatgctttga tgctgatgct gatggccata
     2041 cggggtgcaa aacccaccat gcacgccgtg gcgcaacaag atttcagatt ggatcgtatc
     2101 cgggggaaag aactggggga tatgactgtg ggagttattg gaaccggcca tatcgggcaa
     2161 gcggtcgtca aaaggctgcg gggatttgga tgccatgtgc tggcctatga taacagccga
     2221 aaaatggatg cagattatgt ccagcttgat gagcttctaa aaaacagcga tattgttacg
     2281 ctccatgtgc cgctttgtgc ggatacccgc catctgatcg gtcagaagca aattggagag
     2341 atgaagcaag gcgcattttt aatcaacacc gggcgcggag cgcttgtcga taccggggcg
     2401 ctggtggagg cgttggaaag cggaaagctg ggtggtgcgg cactggatgt gttggagggc
     2461 gaggatcagt ttgtttatac cgattgctcg cagaaagtgc ttgaccatcc ctttttgtcg
     2521 cagcttttaa ggatgccgaa tgtgatcatc acaccccata cggcgtacta cactgagcgt
     2581 gtgctgcagg atactacaga aaaaacaatc aggaattgtc tcaattttga aaggagttta
     2641 cagcatgaat agaataaaag tcgcaatcat cttcggcggt tgctcggagg aacatgatgt
     2701 gtcggtaaaa gccgcaatag aaattgctgc gaacattgat acggaaaaat tcgatccgca
     2761 ctacatcgga attacaaaaa acggtgtatg gaagctatgc aagaagccat gtacggaatg
     2821 ggaagccgac agtctccccg ccatactctc cccggatagg aaaacgcatg ggctgcttgt
     2881 catgaaagaa agcgaatacg aaacacggcg tattgatgtg gctttcccgg ttttgcatgg
     2941 caaatgcggg gaggatggtg cgatacaggg gctgtttgta ttgtctggta tcccctatgt
     3001 gggctgtgat attcaaagct ccgcagcttg catggacaaa tcactggcct acattcttac
     3061 aaaaaatgcg ggcatcgccg ttcccgaatt tcaaatgatt gataaaggtg acaagccgga
     3121 ggcgggtgcg cttacctacc ctgtctttgt gaagccggca cggtcaggtt cgtcctttgg
     3181 cgtaaccaaa gtaaacggta cggaagaact taacgctgcg atagaagcgg caggacaata
     3241 tgatggaaaa atcttaattg agcaagcgat ttcgggctgt gaggtcgggt gtgcggtcat
     3301 ggggaacgag gatgatttga ttgtcggcga agtggatcaa atccggctga gccacggtat
     3361 cttccgcatc catcaggaaa acgagccgga aaaaggctca gaaaatgcga tgattacagt
     3421 tcccgcagac attccggtcg aggaacgaaa tcgggtgcag gaaacggcaa agaaagtata
     3481 tcgggtgctt ggatgcagag ggcttgcccg tgttgatctt tttttgcagg aggatggcgg
     3541 catcgttcta aatgaggtca ataccctgcc cggttttaca tcgtacagcc gctacccacg
     3601 tatgatggcc gccgcaggaa tcacgcttcc tgcactgatt gacagcctga ttacattggc
     3661 gttaaagagg tgataagtat ggaaaatggt tttttgtttt tagatgaaat gttgcatggc
     3721 gtccgttggg atgccaagta cgccacatgg gataacttca caggaaaacc ggtggatggg
     3781 tatgaggtga atcgcatcat cggcacaaag gccgtggcgt ttgctctgcg cgaggcacaa
     3841 atccatgcgg ctgcgcttgg ctatggcttg cttttatggg atggatatcg gccaagaact
     3901 gcggtggact gcttcctgcg ttgggcagcg caaccggagg acaatctcac aaaagaaaaa
     3961 ttttacccca atatagagcg agccgagttg attacaaagg gttatgtggc ctcacaatcc
     4021 agccatagcc gtggaagcgc aattgatctt acgctctacc acctggatac aggggaactt
     4081 gtttcaatgg gaagtaactt cgattttatg gacgaacggt cgcatcatac agcaaaaggg
     4141 ataggggatg cagaggcaca aaatcgaaga tgcttgcgta aaatcatgga aagcagcgga
     4201 tttcagtctt atcgctttga atggtggcac tataagttga ttgatgagcc ataccccgat
     4261 acctatttta attttgccgt ttcataatga aagtattggt tttgtatcag attttggatt
     4321 tgataaaatc tatgctggtc atggacaatc ttttactgga aagtggttaa agcatctttt
     4381 gtaatacatt gcaaattata gtattcaatc tgcccagcgg cagaaaagaa aaaaaccgct
     4441 gttgggcaga cctttcccac gcccattttg tattttgctg gcggcggttt tgagaaatca
     4501 aggccgccgt tcttttctgg cagcaaaagg tgtggcggcc cataggagga tgccgctatg
     4561 ctgttttgct attgccatgc tctaatccac ttccaccact caaaaaaagc cttgcccctc
//
LOCUS       DADVXU010000143         4590 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.150, whole genome shotgun sequence.
ACCESSION   DADVXU010000143 DADVXU010000000
VERSION     DADVXU010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.150"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            186..2855
                     /locus_tag="KB953_002689"
     CDS             186..2855
                     /locus_tag="KB953_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HBB6867601.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2946..3566)
                     /locus_tag="KB953_002690"
     CDS             complement(2946..3566)
                     /locus_tag="KB953_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBB6867602.1"
                     /translation="MFLINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKA
                     MHMVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPG
                     IEKRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTI
                     IKITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            3680..4219
                     /locus_tag="KB953_002691"
     CDS             3680..4219
                     /locus_tag="KB953_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867603.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGGMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            4216..4554
                     /locus_tag="KB953_002692"
     CDS             4216..4554
                     /locus_tag="KB953_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HBB6867604.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         1420 a          790 c          860 g         1520 t
ORIGIN      
        1 atttttaagg gtgggacttt tgattcctac cctttttttg cgtttatttt tgttatacga
       61 acaaattatg aagttattta ttctaaattt tatttttttg tgaaaattag actcttttta
      121 agcagagaaa aacaaggata tgataaactg aatacaactt catagaaatt tggtggaaaa
      181 taaatatgaa aaaaaattta aagtcattct ttagagaaaa cggacgctac gcagcagcta
      241 gtttcctgat tccgtttttg attttagcac ttgtttatct aagtatcggg atctatcctg
      301 gaagcagcag aagtattttg gcaagtgatg ccttttcaca gttttctaat tttcatgcca
      361 gcttccgaaa tgttcttctt ggaaagcaaa gtatttttta tacatggaat gcttctctag
      421 gattgaatta tttatcttta atttcttact atctaggcgg gatcttcacg ccactggtgt
      481 tcttttttcc aaaccagatg atgccagatg cactgtatgt tctgactttg cttaaggtcg
      541 gctgtgccgg attggctttt tggtttcttg cacgtacgtt caagattcct cgctgggggc
      601 atgttgcact tagcgtcagt tacgcattga tctcatttat cactgcacat tcggagatca
      661 tcatgtggct ggatgcattt gtttatcttc cattagtgat tttggggatt catcgtgtta
      721 tggatcttaa acggccgaaa cttttattta tcagttattt actcttgttt ttatccagtt
      781 tttatatggg ttttatgatt ggcgtatttt ctttcttata ttttatcgcc cgtctattga
      841 caaactggtc agaatataaa aaaacgattg ttccttatgg tatcacttct ttactggcag
      901 gcggcgcttc aatgatcatt gtattgccag cagtgctgga tcttcggtca aatggcgagg
      961 cactgagtca gatcactacg ttgaaaacag aagcgacttc ttatctggat ctaatcatga
     1021 aaaatatgat tggtgtttat gacacgacaa aatacggatc gattccgttc gtttacgcag
     1081 gtctgctacc attagtcctc tgtctgtttt attttgccag tcggaaagta ccgttgaaaa
     1141 ataaattttt gtacggcagt cttttcgcca tacttatcgc aagtttctat cttgttccat
     1201 tgaatttgtt ctggcatggt atgcatgcac cgaatatgtt tttattccgt tatgcctatc
     1261 tgttctcttt cttagtgctc ctgcttgcag gaaaaggatg ggaaaagata gaacaagacg
     1321 atcgtgggct gttgttagga atcattttgg tcgtagctgc tctctttgcg gtggcatggg
     1381 gcatcaaacc tgaaggaagc tacacctatg tgacgatgac ttcctttgta ttgacagtgg
     1441 ttttcttagg gatttatgct tttgctgtgg gattttatca gcataaaaaa ctttcattca
     1501 atcatctgag tatattgtta ttattgatca tgtctgctga agcactggtc aatacgaaca
     1561 gtatgatcca tggcattctt gatgattgga attatgcttc ccgcagttta tatactgatc
     1621 cttatcctga cttgaaagct cttgtaaatg aaacaaaaaa agattccgac tcgttttacc
     1681 gcttggaaaa tctggatcca gtatctgcaa acgattcgat caactatggt tacagtggta
     1741 tcagtttgtt ttcttctata agaaatcgta attcttcctc ttacctggat aaattaggtt
     1801 tccgttcaag agggacaaat ttgaatatcc gttataacaa taatactttg ctaatggatg
     1861 ggtttacagg aatcaaatat aacatttcta aaaacgaaag cacgttcagt aaatatggct
     1921 tccgccaagc tggtcaaagt ggcgactata cactgtatca gaatgtcaat gcattgccac
     1981 tagcatttac tgcaccgttg tccgttaatg atgttgagca gccagtgaca gataatttga
     2041 ccagtcagac aaacttgatc aatacattgt ctggtttaaa tgaacagtat tttacattct
     2101 atacacctac tttgaagaat cagttgaata cgacgatcac aaagacaacg actggcgtca
     2161 cttactcaga aacagcagat aaccagccaa aagagctcac ttgggaagtc catgtgcctg
     2221 caaatacaca agcttactta agtttgttcc caaccaattt tgcacaatta gaaagttcta
     2281 gtgcgacgat cactgtcaat ggtacatcgc gaaaaacgca aatcaacatt tctggtcaat
     2341 actatgatct tggctattat cctcaagaga caacagtgac atttactgca agtttttatg
     2401 gaacgaaaga agtaagtttc atggagccta aagtcgttgg tctagatgtt gtagcctatc
     2461 aagccgcaat gaatcagatc aaagaaaaag gtgtcgaaat ggaagccact ggcagaaaag
     2521 cttctgggac tgtaaatacc gctgaagatt ccatgttggt gacaactatt ccttatgaca
     2581 aaggatggaa agctaaaatt gatgggaaac cagtcaaggt cagcaatttc aaagacgcat
     2641 tcttgatgat aaaagttcca gcaggtaaac atactgtcac tttcagttac ttgcctgaag
     2701 gatttacgat aggtgcaatc ctttttgtcc tatgtattgc attgtttatt ctttatgtct
     2761 ggtaccttga ccgcaatcgc cccttttatg gcacgattga tccagcaata gaagaaaaac
     2821 cgcgaaatac acacagaaga agacgaagaa aataaagaaa cgatggaatc tgtgtcactc
     2881 ttctatttaa atggcaaaaa tttcaatctt taaatgacaa aaaccacctg atttttggtg
     2941 gttttttata tacctatgac taaactatct cttgctgctt cgacaacttc tacagtgatc
     3001 ttatccattt gattgattct taaaatccga tcaatctgcg caaataaacg atgaataagc
     3061 ctaaaatttc cttttgtgat cttaatgata gttgtgatag cctcataatc agaaaaatct
     3121 tctagcttga tatcaattcc taaatctgcc catttgtatt ccaaaatatg atgtgtttcg
     3181 tccttactaa ggttatcaaa ttcatgtgca aatcctatgc gagagtaaag ctgcggataa
     3241 cgagacaatc ttttttcaat accaggcata ccgataagaa ccattgccag atcatttcta
     3301 tcgtaaatat ctcttagctg ttctaaatgc tgaagcttta aacgatcaat ttcatcgaca
     3361 ataattaagt caattgcttc ataggtttta ggcacaggag gatcttcttg attgtatttt
     3421 agtaccatat gcattgcttt agtcagacct attctactag ttattgtgtt aatatccgaa
     3481 ctgacacgag acatcttttc agctggtaca gtataaaaaa ctttatttac acttaatatt
     3541 tcatctgttg catttatcaa gaacaagaaa aagtctgaat attcaaaaaa aataacggtg
     3601 tacattttta aaaggagatg tataatcgag atttagaaag cgttttcctc aaaatttaat
     3661 gaattaggaa gtgattacta tgaaaaaatt atttagcgca ctttttattc tgttatttgg
     3721 ctttattggt attgggactt ctggaaatat agttaaagct agcacgatgg ttgaacaaac
     3781 aagtgaaatg gcaaaaactt ttagtcaatt aacaccagaa gaacaaagtt acttcaaatc
     3841 caaaggattt agtgataaag atacttttta cactcaaaag atagagcctc aagatccgaa
     3901 aacacgtttt tctgcaaatg tattgcggtt aaccgcatct actaaaaaaa ttactaatac
     3961 gcaggggcgg acagaatata ttataacaag tcccaattcg gcgatgtatc gtatttctac
     4021 acgcttgagt ttaggtagtg caaaatcata tactcaaaca gtaaatctag ctaattctta
     4081 tggttatggt ggaggaatgt attttacatt tactgggaaa agacaatatt acagtgtaaa
     4141 attagctatg caagtttata atacatttgg tgtagggtca gtctcttgca ctgcgggagg
     4201 attgacaata ggaaaatgat aatttcttac tatgtcttaa atagaagtga tgatgaagga
     4261 ctcttcgttt ttcaatcatt acatgaggat acgatgctaa aagagtttta taatcggatt
     4321 aagaataaac agatagaaac aaatttatat tttttaagtg actctatctt gccttttatt
     4381 agcaacccta aaattcttaa cttgttgcag acaaaagatt tgaaaagttt accgattatt
     4441 gtcattgatg aggaaattgt aaaatatggc gcttatctaa gcgtagaaga attagaaaaa
     4501 ttaactaaca ttggaatgag tattcaggag gataatgggg tacatggaac atagaatgaa
     4561 tatgcacagt tgagaacgga atttgacatt
//
LOCUS       DADVXU010000144         4574 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.151, whole genome shotgun sequence.
ACCESSION   DADVXU010000144 DADVXU010000000
VERSION     DADVXU010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.151"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(1014..1304)
                     /locus_tag="KB953_002693"
     CDS             complement(1014..1304)
                     /locus_tag="KB953_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867605.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1706..1942
                     /locus_tag="KB953_002694"
     CDS             1706..1942
                     /locus_tag="KB953_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867606.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1949..3901
                     /locus_tag="KB953_002695"
     CDS             1949..3901
                     /locus_tag="KB953_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HBB6867607.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="KB953_002696"
     CDS             complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="KB953_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HBB6867608.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT         1563 a          804 c          755 g         1452 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattta agtagttcag ttttggagta caaaaagtga
     1501 aaatgtaaac ctaaatgagt atcaaattgc aactgaattt gatactcgtt ttttattgtt
     1561 aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag ttaaaatgta tataaaaatg
     1621 cgaacgtgaa ataattattg taggaatgca aattggtgtg ttaaaatggt tacaatttac
     1681 taagcaaaaa ggggtgcatt ttcctatgca atttcttgag ccaaaaaata agaacgctaa
     1741 gtctgtggat tgggaaatat cagagcaagt aagggtgata gttaagcaat acgctgaata
     1801 tgcagagcgt actgaaagcg aagcagtgga tgaattctta ttgaacattt tagacgataa
     1861 gaagtttatt gaatggattg ccaacaagcg aagtaataag cggattgttg aaaaaatggg
     1921 tatcaaagat agagtgggtt gatagacaat gaccaagctt aaaagattag ccacgaagga
     1981 agatgaaatt gtagatgtta aaattccgat ttcaaacgaa gaattaaaag atagggctaa
     2041 acaatatgat ttattaactc caaaacgatt tgcaacaagg tataacaaaa tgttgttctt
     2101 gccaacgtct tttaaatgga acggttctga atatcccatt caatataatt actgcatcaa
     2161 tccgttttgt tgtaattttg ggaaagaaca gcacaaattc aaagatgtaa aagggaaacc
     2221 aagtcgatat aaaatgactg gtagttccaa agataaaggt cataaaggta tgtactgcaa
     2281 tgacaatcct attggcagag gtgtatcgca aaactgtaca gtaacccctt tatctaattg
     2341 gtctgttgtt gaagaaatta aacgccttat agaaatcaat agcatccaag atgttgagcc
     2401 agattatcaa tttcataaag aaggctgctc ggaagaagaa tcaaccccat tcaatgagcc
     2461 gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct caacgctatc aatgtaaggc
     2521 gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa accactactt atcaccaaca
     2581 aaagaacacc attcttccaa tgttcgctaa gatggttgtt ggaagggttt cagtcagtcg
     2641 tacttgtgat attttaggaa ttggagttgg aacctactat cataagctgg agtggctgta
     2701 taggcgttgt ttggagtttt tagaacgata tgagacacaa ccccttcaaa caaagaaatt
     2761 taacgagatg tggcttaata cggacaagat gcactactac ctaaataacg tccgtaagaa
     2821 aggacaaggt tcaaagaaat atacggggtt tgaagacctt aatatgcaaa cgtatatagt
     2881 tgtatctgct gaggtcttat caagatatgt gttccgttct gatgttgcct atgattggaa
     2941 tatttcaatg gatgaattaa atgaagatac aagaaaattc aaagaagacc atctcaatac
     3001 ttttagtcgt aaaaatgata ggttagattg gtcttattat ccacaagaac catccgctaa
     3061 cgatagcgaa aataggaatg cttatcttca cgaattaggg aaaattacta ataggagtag
     3121 atttgtcgat ggtttaaatg ttgatgctcc ttatactaca actgctcact attggttaat
     3181 taaacaaatg gttaatgccg acgaatggcg aatgattagt gatgatgact tctctattag
     3241 aaacgccttt tatagagtat tcacgaaaga attaaggttg agcgatgcac atcattttat
     3301 ttgccaagtt aacaagacga aaagcagaaa acaatgctta aaagaattcg gacaagcaaa
     3361 agcagaactg ctcgattggg gagatatcag aggattcaaa acaaagtttc tacggacttt
     3421 ggcatctcat tatctaactg aattactaac aagtcatcaa ttccacgaag aggctattag
     3481 taaagatgga gaaagatatc gcaaatatgc cgataaccct atcaaacatc cattagccac
     3541 taaggataaa ggattttatt cagttgattg tagaacggat ttatctgctc tggaaccaaa
     3601 cgaaatcgca aagatgctat taaatgttaa tgaccattcg accaatagct ttattcaaca
     3661 gataagacgt tatatatcat ctcttgaaag accattaaca acagctcgtg gtgataagaa
     3721 gagctatatt tacgctaact tcaatccgaa gtatgctcaa tttgcgatta caattctaag
     3781 aacttactac aacttttgtc gcccttttaa atcagcggat aaaaaggttt tgacacctgc
     3841 tcaaagatta gggattacag ataaacaatt tgattggaaa gatataattt atttcaaatg
     3901 acaaaaagag cttggagtaa tcccaagctc ttgtgcttta tctttactgt tcttcttcaa
     3961 ttaacgcacc cgttaactca ataagttttt tctttcatat acatgaaaat agtagttata
     4021 cggattttta tcattcttta tccctttttg ggcaaatacc tcattccatt cctcataatt
     4081 cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt atttttgtga tgtacatttt
     4141 ctcaacataa gggaaaaaca aattataaat ctgttctcct ccgaaaataa aaatttcttc
     4201 ttcgttttta cataactcaa aaacatcttc tattgaatga acaatttcac aaccattaaa
     4261 ggtaaacccc ttatctctcg tcagaataat atttcttctg tcaggtaagg ctcttccgat
     4321 tgattcaagg ttcttcctac ctaatattat cggatgtccc tttgtagtat ttttaacata
     4381 ttcccagtcc ttgggaatcc tccaaggaat gtcattctct ttgccaatca ctctattctt
     4441 atccatcgca gcaatcaaag aaactttcat ttataacaca gcctttttaa ctaatttacc
     4501 atctaatttc ttatttctat caaaaaacct tttctcatta tcctgcccgt tagttgaata
     4561 agcaattttt tcta
//
LOCUS       DADVXU010000145         4491 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.152, whole genome shotgun sequence.
ACCESSION   DADVXU010000145 DADVXU010000000
VERSION     DADVXU010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.152"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            92..1426
                     /locus_tag="KB953_002697"
     CDS             92..1426
                     /locus_tag="KB953_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBB6867609.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            1467..2378
                     /locus_tag="KB953_002698"
     CDS             1467..2378
                     /locus_tag="KB953_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBB6867610.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            2375..3229
                     /locus_tag="KB953_002699"
     CDS             2375..3229
                     /locus_tag="KB953_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBB6867611.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            3226..3843
                     /locus_tag="KB953_002700"
     CDS             3226..3843
                     /locus_tag="KB953_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HBB6867612.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            3845..4363
                     /locus_tag="KB953_002701"
     CDS             3845..4363
                     /locus_tag="KB953_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBB6867613.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
BASE COUNT         1349 a          764 c          907 g         1471 t
ORIGIN      
        1 gaatcatacc ttataaattg ttattttcag aacaaaatga tatcgttata ttaaaaccgg
       61 catcggttac attaaaattt ggaggtaaaa tatgaaaaaa ttattgttta cagggttgct
      121 ggcaagttca gcattgtttt tattaggagg atgcggatca agtaatgcaa gcgatgccag
      181 ttcaagtgca gcaaattctt ctgaaaaaac aagtgatgga agaaaaaata ttgatttctg
      241 gtccttttgg ggatccggtg cacgaaaaga agctatcgaa gaaatcattg aggatttcaa
      301 tcaatcacaa aacgagatcc atgtaaatta taagtatcag ccatggggtg acatctggac
      361 taaatcttta tctgcaataa cagctggaaa tgcacccgat gttattgtac aggacataaa
      421 ttccgttgcc caaagagcag aagcacagca agcaactaat ctctccaaat atgtagaaaa
      481 agacacttct aaggattttt atccgcagct ttgggataca gtagaatatg aaggtgatcc
      541 ttatgcgatc ccattcaata cagatacaca agtcattttt tataataaaa agttatttaa
      601 agaagctgga atcagtgaac aagatctacc gacaacttgg gatgaattag aagaaacagc
      661 acgtaaacta gataaaaaag aaggaaaaga atttacacgc atcggttttt atccattatg
      721 gaatttaggg gctgatgtat gggcgctaaa cgcagataac gggactagtt ggtttgacaa
      781 agatgaaaaa gtcaagattg atactaaaaa taaagtggaa gccttggagt ggatccaaga
      841 ctggcaagat tattatggga aaaatacgat caataaatta gaagccgaat ttgggtctgg
      901 tgtatctgat ccatttattt ccggattagt agccatgcgt gcccaaaata tcaattatta
      961 ctctagtttg atggaaaatg caccggatga ttttgatttt ggcgtgatgc agctcccaga
     1021 aaaagaagaa ggttcaggcc attggtcgtg gggtggcgga ttcgtcttgg aagtaccata
     1081 cggagcaaaa gatcctgagg cctcttatga attcatcaaa tatctttctt ctccagaagt
     1141 acaagaaaaa ttcggcgaaa aaagttttga tatcatggcg agcaagacag ccaatgagaa
     1201 tttagtgaat aatgacaacc taacggataa aggaaaaatg atctatcaaa tggcggacga
     1261 aaactttaaa aatacggtga tcacaccagt tcctttagat gctccggatt acacgacatt
     1321 agtgaatgaa cagattgacc agattctttt aggaacaaaa tcaccaaaag aaggattggc
     1381 agatgcacaa aaagcagtag aaaacttagt caagcaaaat caataatcca tttttgactg
     1441 tctaggtaca gaggaggcaa gaaaatttga agcgtttatc tatccgaaag aaaaaagaag
     1501 caagatgggc ctatttattt atctcgcctt ttattatcgg gttcttggta tttatgctag
     1561 ggcctatgct tttttcagtt attggtagtt tcaccgatta taatttgaca tccaagatga
     1621 atttcattgg tctagcaaat ttcaaaagaa tgttcgttca cgatgattta ttttggaaat
     1681 cgttatacaa caccgtttat ttcgtcatct tcaatgtacc gctgacgacg attttcagtg
     1741 tgtttttagc cacagtactc aatcaaaaaa tcaaaggaat cagtgtattc cgtacagctt
     1801 tctatttacc ggcagtctta tcaggagtag cagtctacat cttatggatg cagctgcttt
     1861 ctccatctgc aggattgatc aacacatttc tgggctggtt ccatatccct ggaccagctt
     1921 ggctgtttga tccacaatgg acgaaacctg cgttgatttt gatgaaaata tggagttctg
     1981 gaggagcaat gctactttac ttagctacat tacaaaatgt tcctagatct ttatatgaat
     2041 cagcagaaat cgatggagca ggcgtttggg gaaagttcaa aagtattact ttgccattaa
     2101 tcactcctat tattttttac gatgtgatca cctctacgat cggttctttc caaatttttc
     2161 aagaagctta tgtcatgacg aaaaatggaa caggcggacc tgcaaattct ttgctttttt
     2221 ataatctgca tatgtggaac aaggcgttcg tagcttttga tatgggttat gcgatggcta
     2281 tgtcaatgat cctgtttctg atcgtgttgg ttctgacgtt tgtcaatttg aagcttgcac
     2341 ctaagtgggt ctattattct ggaggtgacg gcaaatgatg gattatttca aaaagaccag
     2401 tgtgaatcgg caaacaaaaa aaatactaag ttatgtcctc atgacagtaa taggaatcat
     2461 tttgatcatt ccattgcttt ggatggtgtt tacttcattg aaaccaatgg aagaaatcgt
     2521 tcgttatcct ccgacctttt tcccagaaaa aatcgtatgg gaaaactatc tggatacgat
     2581 tgccgccttt cctttttggc gctatgcaag aaatacatta ttcatcactg tgctagtagt
     2641 gatcggtaat gtcctttcaa attcttttat tgcttatgga tttgcgaagc ttgattttcc
     2701 tgggaaaaag ctgatgtttg ctttggttct ttctacgatg atgatcccgg ggtttgtaac
     2761 gatgatccct caatatgtat tattctccaa gatcggctgg gtaggtactt atttgcccct
     2821 gatcgttcca tcgttcttcg gcaatgcgtt caatatattt ctgatgcgtc aattttactt
     2881 atcgatcaat aatgaattga tcgaagctgc tgaaatcgac ggagcgaatc atttgtatat
     2941 ttggagtcat ttgatgcttc cattgacgaa acctgccttg attaccattg cgatcaattc
     3001 cttcaacgca gcttggaatg atttcttagg tcctctgtta tatatccaag atcaagaaaa
     3061 atacacgtta cagattggct tgcaagtttt tcagaaccaa gcaacgacac aatggaatta
     3121 tctgatggca ggagcaacac ttgtcttgat cccgacgatt ttgctgttct tttttgcaca
     3181 acgttatttc attgaaggga tggatttgac aggaggttca aaaggatgat gaatcgatcg
     3241 attggtaaaa aagccaatga ttatttctta gaaatcattg aatggattcc acgtcttatc
     3301 catttacagc tactatggtt cctgtgcgtc ttgcctgttt tcacattagg aactgcaagt
     3361 cgaacagttc tgtatatgat ttatcattac aaaaaaaatg aagagaaaaa gctcggttca
     3421 ctattctgga aaaagtttcg tgagaacttc tctctttatg gaaagcaaga tagtttagca
     3481 agtgtttatt ttcttcttct attgatcgat tatcagattt tccgctattg gggtggggga
     3541 attggctata cattgatgta tactacgttg tttttgcttc tattaagcgc gcttctcttt
     3601 agttacaaaa tcttgcttca actggaacaa aaagaagctt cttggattac tgcatttttc
     3661 ttgtttttta atgatttcaa aagcgcgctt ttctatttgg caggcacgtt gatccttctt
     3721 gtcgcattct ggtttgcagg accaatctat tttgctttat taggtttcag tttcttgttt
     3781 tattttcaag tcctgctttt tatccatcgt acgcaagaaa aggttcttaa aaaggaggaa
     3841 taacatgttg cgtttgattg ctaaaatcga tgatagtatt atcaacaagt tcgccaagca
     3901 acggaaaacg gatagtcgaa aaatcaattt tttgaaattt atcagtaata tctctaatgg
     3961 tggttttatt tggttgctcc ttgctgctgt tttagtcttg atacctgata ctagagaata
     4021 tggtctagcc attcttatcg gcagtttgat ctattttttt gttggtttgc tttttatcaa
     4081 aaaaacgaca agacggaaaa gaccttatga aaaaaatcgt cggcttgtcc tttacacaag
     4141 taaaccgaag acttcttctt ttccttcagg acatacagcg ctagcctttt tatcagtagc
     4201 tgcgttgttc gaattaagtc cagttgcagg aatattagcc tttttatttg cttgtttgat
     4261 cgcttattcg aggatttttt tacttttaca ttatcctact gacattttag gaggagctat
     4321 tttaggaagt gttattggca tcctatgcgc tgccgttatc tgaattaatt gtacgaataa
     4381 agcatgagtg cgtaaatgcg catttgtgct ttattttttt gtttcgagga aagcgtagtt
     4441 gtttattcat ttgtcttaat aacttaagtt ttgttaaatg agaacgttat a
//
LOCUS       DADVXU010000146         4477 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.153, whole genome shotgun sequence.
ACCESSION   DADVXU010000146 DADVXU010000000
VERSION     DADVXU010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.153"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            631..744
                     /locus_tag="KB953_002702"
     CDS             631..744
                     /locus_tag="KB953_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAAX protease"
                     /protein_id="HBB6867614.1"
                     /translation="MYAPGTILLTIAYLTANRSLAFIIAIHILNNVLGFVL"
     gene            complement(832..1125)
                     /locus_tag="KB953_002703"
     CDS             complement(832..1125)
                     /locus_tag="KB953_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HBB6867615.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(1528..2706)
                     /locus_tag="KB953_002704"
     CDS             complement(1528..2706)
                     /locus_tag="KB953_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBB6867616.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(2810..3124)
                     /locus_tag="KB953_002705"
     CDS             complement(2810..3124)
                     /locus_tag="KB953_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6867617.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            3231..3446
                     /locus_tag="KB953_002706"
     CDS             3231..3446
                     /locus_tag="KB953_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867618.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            complement(3746..3988)
                     /locus_tag="KB953_002707"
     CDS             complement(3746..3988)
                     /locus_tag="KB953_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6867619.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(3975..4403)
                     /locus_tag="KB953_002708"
     CDS             complement(3975..4403)
                     /locus_tag="KB953_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBB6867620.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
BASE COUNT         1418 a          841 c          663 g         1555 t
ORIGIN      
        1 ataaaaaatc atcaaataat cctttacacg ttattctaaa tttaattttt attattttat
       61 tgatgtatag aaagtttggg gctttttttc tctttttatt ctaaggttat tttagtcttg
      121 tcctttaata caaagatttt atttttaaat aaaaaatact ctttttttac gattattgaa
      181 aaaaaaagat aaaacttgat taattgaagt attgcttaaa tttggaagct atgagggaag
      241 aataaaagag ttaggacggg attatctact atttttcttt gttttaaata tttgttatct
      301 ttgaacataa aagagaaatg tgtcgactgc agtgttgtgt ccctcatcac gaataaacgg
      361 tgttcaatta gctgatcttg tattcactta aaaaatggta aagtacgagt tgctattccc
      421 cattttcaat tttctaacgc tcaggacaaa atactgtttt cacaacctca ttggaataaa
      481 agcatatata ttgtttcaag aacgaaatct tgttatcttt ttaaagggca tctgtccaaa
      541 atctaatttt aggaggtacg ttttggtatt tattcgtaca aataaaagag aattatcttt
      601 attcatgtaa cgcatccaat tgattttttc atgtacgctc ctggaacgat tttactgacg
      661 attgcttatt taactgcgaa tcgctcatta gcttttatca tagctatcca tattttgaat
      721 aacgttcttg gctttgtatt atagatacag atgacaaaaa aagttcaagt atgcggtgta
      781 gaaccaatat cgcatacttg aacttttttc aatgagtata aattaaaact tctaattttc
      841 aattcccatc atctgatatg cccgaccgtc tgaacttctt ttgaaaaagc caaatgtgat
      901 tagataacgt ctttgttcga cataatcgcc actagaaaat acgttcagca gtatcgaatt
      961 aacctctgtt tctgtataag tcactccatg gtcaaagcgt ttcaagactt ctttcaatag
     1021 gatgtatttc ttttttgttg aacttggtac ttgttttact tttccatcat tcatgaatgt
     1081 gtctaaaact ttcatctttt cttgctccgt ataagtataa accatcttct ttctcctttt
     1141 gattctaaga atactccatt tatgttagaa tattctggat tttttattaa agcggttact
     1201 cttaagaaag atattatcat agtgctcttt tttttactac tacttatagg tagatttatt
     1261 ctaatccatt attctcccct accaataatt aaagaaaaaa ctggatcagg gtgttcaaat
     1321 tacatgctaa gaaaaaagaa cccttgaata ccaagggttc tttctcagtc tgataacaat
     1381 gattaacgac ggatttcttt gatacttgtg aaaagtcgtt aaagtattga tataaaagga
     1441 tttttattac ctctgactac atattgacta atttgttaaa aaatcgtttt tcaagaatcc
     1501 tgtaatacca gtgctttcac cgttttttta actctctaaa gaactaaaag caatcagagg
     1561 ttttaccttt tcatttgacg ttaagtggcg ataaattttc gaagttgttt ggtcagtatg
     1621 gcctaagcga tctttcaaga cttcccagct acagtgttcc gctactaagc ttgccattgt
     1681 atgacgagct aaatgagtat gaaatggctt attaattcct gagcgattca cgcaccgttt
     1741 gacgtgatca tttacatact ctcgacgata aggaacacca cgtttactag aatcaagata
     1801 cacaaacaaa tatgtttctt ctataataaa cctgtaatgt tgcctcatct cacccaaagc
     1861 cttaatcaac tgttgtagct tttccataac tagaggagtt actagaactc gtctagcaga
     1921 actaactgtc ttagtagttt caattcgaaa atccttactc ttacctttaa ctgccgtttg
     1981 cttatagaac agaatttcat ttcttttgaa atctatatct ccaggctgta gagcaagcgc
     2041 ctcgctgact cggcagccag tgtaaaatag aacatggaaa agtacagaca tcggtaattc
     2101 ctcatgaaca atgccataat tcaaaaaagt tctcaattca tcgacagtaa gaaacttgtc
     2161 ctcaatatta tttatggctt cttttaaatc agttaccgtt aactgatatt ttggcattct
     2221 cacagacttc attggatttt cacttatcaa attctttctt tgacaatatt taaaaatcat
     2281 atttagaact tgaataagtg attgtaaact atttttggca tagccaacat ttgttccatt
     2341 aacgccacat tcctttaagg taaaaagcaa ttcttgaagg cactctggtg tcaaatcaga
     2401 caacttttta tcaccaatcc atcgaacaat aatttttaat tgttcctctc gcttatcata
     2461 ggttatttcc ttttgttcat ttaacgaacg gtaatattgg aaccattgct cacctacaac
     2521 actaaacttg tcattgcttg caacaatttt tttcttaagc ctttgttgtt tctccatttc
     2581 cagataagcg agttgcgcct cacgttttga attaaagcca cgtcttgttg ttcgatatgc
     2641 ttgaaagtac catgtgatcc taccagtaga actgatttgc ttcttgaatc gatctgtttt
     2701 ttccatttat tgattcctcc tattttgaag gaacccaaca agcagaaacc ctttggaaat
     2761 ctttctatct atgtcatcat tgtaccgcat cttagaaaga aaatgtaact tataaagatc
     2821 gaagtagtcg caatttttct cgatagtcat tcgaatcact ctttctcatc cattctaaaa
     2881 catcatcacg aaaatagtgt cctcgaatcc cgtaccaaca gggaggaaac tcaccaagtt
     2941 ttactaaacg gttcagtgta ctatcactta tctgaaatag tcgctttaaa tcagtatttg
     3001 ttaataccag aggtaaatga ctgactgcaa gcaatttttc cgtgtaatct tccagttttt
     3061 tttgtaaaat tgaatagaac tctatttcca gagaattaac atttgtttta acttccaact
     3121 ccatcaccct tctctctcaa aatcaaaacg accaccacgg taccccttaa aattgtaaaa
     3181 aaacactcca gtactccatc aaaacggact agaaaagagg aatttttaat atgaaacaag
     3241 gaattgcaga cataaaaata attaaagaaa ttcttgaaaa atctacggct aatgcaatag
     3301 cattcggaac aggaatcaac ttgagtacag taaaaaaact taaaagtgga gagagagctg
     3361 aagaaaaatt aaatcttgcc gatgctatta aaatcacaga atttggcatg aaaaatatgc
     3421 caaccaaaat cgaaatatgg aaatagtagt ttttctgatc tatcagctgt tttgccaaag
     3481 cacataggaa tctcacctcc ccccagtaat ggcgggccga tttggttacg gaagtatcat
     3541 tatcgtagtc gcgattctca tattaaaatt tgtcgcttaa aagctaacct tgtcatggcg
     3601 taactagaga atcaaaatct acatgtactg atagatttgc tattcatttg tcaaagagcg
     3661 ctaggctatt ttcagcctat ggaaatcctt tattttgacc cgataaaaaa cttccataga
     3721 atgaaaagga aagggaaaaa atactttaac gagttgtatc aaattcaagt atcttctcaa
     3781 tcaattttgt ttccagttgt ctacgcatat attcatctac atacataacc attccttttt
     3841 cttcttcata aactggtttc atggctaacc gtgaaatata attttgatat tttgacagaa
     3901 cgatggacat ggcaattgaa tcaccagaag ctgcggcttt gattgtcgtt agtggtatga
     3961 atggtatata tcgtttattt tgcttcatat ttgacaccca aatactttcc tagctcttcc
     4021 aaagccattt gtcgttggta ccaaacactg ccgagacttt tcccaataat atcgccaatt
     4081 tcacgatcat taagaccgat gaagaaggac ataaaaatga tttcttgtct ttgactaggt
     4141 aatgctgcaa tcgcatttgc caactcctca ttggaaataa agattttcat accaaggaca
     4201 ataaaagtac cactactatt caaatttggt tccaaataat agaactctgt acctaaccct
     4261 ttttgttcca atatttccat tgatatttgc cgttggcgaa atctagcgta cttcttttga
     4321 atatttaacg cctcattgca gactactttc ttacaaaaac tgtcaaacgt atgttgaatg
     4381 tgggtttcct ctgagtcgcc cataatattc tccccctcaa aaaattcatt caactcgttt
     4441 tacttctatc cctttccatt attactacaa tctagga
//
LOCUS       DADVXU010000147         4386 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.154, whole genome shotgun sequence.
ACCESSION   DADVXU010000147 DADVXU010000000
VERSION     DADVXU010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.154"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..356)
                     /locus_tag="KB953_002709"
     CDS             complement(<1..356)
                     /locus_tag="KB953_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867621.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRINESDYNKRDQVLL
                     VKLNEEISVEEAVRTF"
     gene            complement(616..1164)
                     /locus_tag="KB953_002710"
     CDS             complement(616..1164)
                     /locus_tag="KB953_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867622.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1165..2022)
                     /locus_tag="KB953_002711"
     CDS             complement(1165..2022)
                     /locus_tag="KB953_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBB6867623.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2308..2594)
                     /locus_tag="KB953_002712"
                     /pseudo
     CDS             complement(2308..2594)
                     /locus_tag="KB953_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2584..2913)
                     /locus_tag="KB953_002713"
     CDS             complement(2584..2913)
                     /locus_tag="KB953_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBB6867624.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2990..3262)
                     /locus_tag="KB953_002714"
     CDS             complement(2990..3262)
                     /locus_tag="KB953_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HBB6867625.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3262..3525)
                     /locus_tag="KB953_002715"
     CDS             complement(3262..3525)
                     /locus_tag="KB953_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBB6867626.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            3759..4386
                     /locus_tag="KB953_002716"
                     /pseudo
     CDS             3759..4386
                     /locus_tag="KB953_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
BASE COUNT         1328 a          873 c          586 g         1599 t
ORIGIN      
        1 ttaaatgttc ttactgcctc ttccacacta atttcttcgt tcaatttcac taataatact
       61 tgatctcgct tattataatc gctttcattt atcctaagag tcaaatcttt ttccaccata
      121 acttttacac ccaataaatt ttgcggttta tttttctctg ttgaaattct ttgtgtagcc
      181 tttaaactga caattagctc attgggtaca tactctattt gagagtcatc taattggctc
      241 ataatccttt ctttttggtt agtatctgca gttacttgtt tagtatgagc catgaaccca
      301 aacatactca ccccaacaac caatcctaaa tatattttct ttttaaactt tttcatctac
      361 atctcctcct aatagtttta ttttagagtt agctctatta cttatttact aactcaacaa
      421 cagattatca tacacttgtt aatgaaatca caatatctca ttaaaacaca ccctaaaatc
      481 ataaccgttc ctatgctcat tttttggatt ttttaatcac gtaaccagaa aaataaatcc
      541 cctctatttc acgcaaatca aagaagaact aagtaactac cgtttaaagg tagttactta
      601 gttcttcttt gattattatc taactttatt attcattccc caccctttgc gcagtgagtc
      661 ttcggtatct tgttttatct gactggtagt tcttattatt tctccacgat catctaattc
      721 tggaacaatt ggatattttt ctctgaacaa gtctagttct ttcaccacgt cattaaacaa
      781 atcttgctta tcttcttgcg taagattctg tttaatatac tcatctaaag cttttctaag
      841 caattcatct cttgtcatat tagtctcata cttttctact aaaactctgg aagtcgcatt
      901 taacaaagtc gacagtttta aaggatgctt ggtggaataa accttatcaa actttctgat
      961 tggtgcgcca actttttttg ttcttcctct actctgtctt tcaacattgg aggaagtttt
     1021 tatttcttga acttggtgca cttctaattg cttattttca gatggtgtaa ttgcttgctg
     1081 ctttttcttt gcttctgctt ctttttttat tttggcgagt cctttgttgt tcccaaaaat
     1141 atcaagtcct ttgatgtcag ccaattactc cacctccgca taccaattct cgtgttcttc
     1201 caattctttc aaaatactga taaataattc gtggcttttg cgatcccatt gttttaagag
     1261 acctttcttt gtcttctcca tcgtaatacc tgatccgtcg tatcgtttta agcgcgcatt
     1321 tttgagtaca acatttttaa tcaaatgttc cccataaatc tcttgggctt gtttatacat
     1381 ttcctgatct actgcgtcac cgctttccaa catgaaagga ataacaccag caacatataa
     1441 atttgtatca aattctttca caaaaaagtc catatactca agatattgca tcgcatttct
     1501 catcgatttt acttgggtct gtaagacgac gattgcccaa tccgcaatgt acatcgcgga
     1561 tttactatat acagaaatag aaggtgggac atctaaatag atgacgtcaa actcatcaaa
     1621 taatggttct agcttttctt tcatatattt gaattgggat aatgaatcat tatattggtc
     1681 tttgatccaa tcatcataca tttcaaagga catgtctgct ggaagtatat agaggttatt
     1741 gattacttta tagacagcct ctttaggtgg aagattgttt atcaacacgt cagtcacagt
     1801 attttcgtaa acaatcggtt caccttctcc gcctccttct tcgttagtga agttcatatg
     1861 cgttaagttc atcacatcag aagtatttcc ttgcatatcc aagtcaatta tcaaggttct
     1921 ttttccttca ttaactgctg attcaaaccc taacatttgc gcgcacgtac ttttacctac
     1981 gccaccttta aaattaccga ttacatattt cttcgctacc acgttcctac ctcttttctt
     2041 ttagcaatat tcatttgata ctattataac atatttattt ttagttacta ccaaaaaaaa
     2101 gatatatatg tactacctta taattaataa gtaaaaggta gtacataaat aaggaaaagc
     2161 tgaaatatat tgatttatag ctttcctcaa agtaatgacg ttatttaatt tatcgagaag
     2221 cagagaatac tcgcgacttt agtcatgtag acgaatcaga ttttcgataa aaaagaagcc
     2281 gtccttctta agaacgacaa caaatcacta gattaatttt ttcaactgtt cataaaagtt
     2341 ttcatgcgtt ccacataaca atactggaat aaccttgttt tctataatct cgtaagctac
     2401 tctatatgtt gtgcccgcat atttgaaccc actagaccaa atacctgata aatccccagt
     2461 tttttgttca cccttatatg gatcaatcgc aatttcatca taaattaaat tcaaaaattt
     2521 ttctttcaat ggcttctctt ttagcttttt aaatacttaa tgacatgttt tgaaggaacg
     2581 atttcataag ccaatttctg ttgccaactc ctctttagtc attactttag aattagtaag
     2641 tgtatcttca gcaatatctc taaaggaaga atgcattttg ttttttctat tcttaaattc
     2701 tacaagaatc tcatctttat cgtagccttc tgcaattata tcagacaaaa tatcttcgct
     2761 aaaatcataa aattcttttt gtggttcaac aaattcataa agaataccat tctctaccaa
     2821 ctttgcttca acttcgactc cattaggaac attaaaatct ttaggtactg tcaacatgat
     2881 tgaattgcct tgttttcttg tttttgtaat catttaaatc cctcatttct aacagtatgt
     2941 aattacttag taaatacatt ataccacgag ataatgtatt tacaagtaat tactgtgact
     3001 cgtaatgcgt ccttgctgaa taaatttcaa ccacgtgttc atcttcatct accttataaa
     3061 caacccggtg ttgtctattc aatcttctag aatacctacc ttcatgtgta ggttttaact
     3121 tctcaaatga ttgggtaggg agatagggat cttcctttaa agtctgaata accttctcaa
     3181 attgaggttt caaattagac tgtttgattt tctttaaatc agctttagcc gagtttttaa
     3241 tcatcacaga atagttattc attataagtt atcccaatca atttcatcaa cattagtaaa
     3301 tccactatca tctttttctc gcttacgaac aacacccaaa gtgcctattt gctcaagtaa
     3361 tagcgtttct ttgattgctt tccaatcatc caaaccaata agcaccgcat tattttcact
     3421 gcgctcacta atgatttcaa tttccgtatg attctcattt acatctttca acaacttata
     3481 aaaatttttc cgtgctgagc taggatttaa gactgttttt gccatatcta ctccccctct
     3541 aatttatatc tccattctaa cgtacttaat taagtacgta aattcgattg ttttgtttaa
     3601 attggagtac caaaacagca ttgtaaagtg ttatccgttt cctttatacc cgtggtgcta
     3661 gttaattttt tcctagagga aaatgtctaa acatgttggt aaatcaacaa aaaaacgcat
     3721 agagtagtct caaaactccc aagaaatgag gactacatat gcgttcatca aaatatactg
     3781 aacattatac agatactttt ataacattca aggaaattat gagaactgtt tctaacatat
     3841 accacaattg tgtaccagat aagattaaaa accgaagaaa tactgatcaa ctgaagtaac
     3901 gcgatacagt gattattgct tgtgttatat ggggcataat taatggctat actagccaaa
     3961 gagccacgta tagagcggtt tgttccgtct tatttcctaa tggtgacttt cctagtagga
     4021 gtcgattcac tcgcttaagt tcaaacttag cttacactat caagattatt cgatactttt
     4081 tcatcaagaa gctcaccaaa ggtgaactag tcggaattat agatagtttt cctagtccat
     4141 tatgtaaacc agttagaaat agacaagcaa aactattgaa tcaaatagcc aaagttggct
     4201 ataactcaac aaaaaaatct tatttttatg gtcttaaaat tcatatgatc gttactaaga
     4261 ctggctttcc gattacttac tcaatcacga atccaggtgt ccatgacgtg aaagtgttag
     4321 agactttatc ggaagaagcg aatcttccca atatcctagg ggacaaagga tatattagcc
     4381 ataaaa
//
LOCUS       DADVXU010000148         4365 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.155, whole genome shotgun sequence.
ACCESSION   DADVXU010000148 DADVXU010000000
VERSION     DADVXU010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.155"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            38..919
                     /locus_tag="KB953_002717"
     CDS             38..919
                     /locus_tag="KB953_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6867627.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            932..2542
                     /locus_tag="KB953_002718"
     CDS             932..2542
                     /locus_tag="KB953_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HBB6867628.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2619..3278)
                     /locus_tag="KB953_002719"
     CDS             complement(2619..3278)
                     /locus_tag="KB953_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBB6867629.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVALYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3561..4226)
                     /locus_tag="KB953_002720"
     CDS             complement(3561..4226)
                     /locus_tag="KB953_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HBB6867630.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
BASE COUNT         1167 a          853 c          868 g         1477 t
ORIGIN      
        1 tttagtatat atttttagaa aaaggagtgg ggtttttatg gcagaaatcg tcaaagtcac
       61 acatttgaaa aagagctttg gtcgtttcga agctttaaaa gatgtctctt tcaccgtaca
      121 ttcaggtgaa gtcgttggat ttatcggacc aaatggttct ggaaaatcca cgacgattcg
      181 gacactttta gggattataa aaaaagacgg cggaacggca acgatttttg ggcaagatgt
      241 atgggaagat agtttatcta tccatgaacg tctttcttat gttccaggcg atatctcttt
      301 gtggggaaat ttgactggag gagcgatcat cgatctcttc atgaagcttc atggaaaagg
      361 gagtgctcaa aaaaaagatg aactgattca acgtttctct cttgatccta agaaaaaagc
      421 aaaaaactat tcaaaaggaa atcgccaaaa agtgggcttg attgctgctt tggctgttga
      481 ttcggatctc tatttatttg atgagcctac ttctggttta gatcctttga tggaaacgat
      541 tttccaagaa gaagttgaaa aattgaaaga acagggcaaa tccatcctac tttcttctca
      601 tattctcagt gaagtcgagc gactagcgga taaagtcgtg atcatacgcg aaggaacact
      661 cgatgaagaa ggaacactcg atgaattgcg tcatttgact cgttcgacca tcactataga
      721 aacagaagca gagaatagtc acttagcaca attgaacggt gtccatgact ttgtgcaaaa
      781 aggtcacctc gctcaatttg ctgctgataa cgaagctttc gatgtgattt tagctgaagc
      841 gtccaaatta ggtgtgaaaa aatttgaatc tattccaccg actcttgagg atttattcat
      901 gagacattat gaaggatgag gaggaagcga aatgactgat aaattttcac gctggtccac
      961 ccttttttct caaaacctta gccgtgactg gaaaaagatc ctgatatggc tagtcggtgt
     1021 cactgctttt tctggtggtt ttgtccctgc gtttgaagaa ttgacttccg gacaaggatt
     1081 agctggatta tacgaaacct tgcaaaaccc ggcaatgatt gcaatggtcg gaccaactcc
     1141 agcttcgaat gctgctgagt atactttagg agcgatgtat gcaaatgaaa tgttgctttt
     1201 ttgcagtttg tttgctgctg tgatctctgt tttacacgtg gtgagccaaa cgagaaaaaa
     1261 agaagaactt ggtctgattg aacttattcg ttcctttcga gtcggacgtc aagcaaactc
     1321 tttagctgtc atcgctgaaa gtattctgat caatttgctt ttagcaatat cgatcagctg
     1381 tacgatgatt tttttccaag cggattcgat cactgcagaa ggtgcttggc tttttgggct
     1441 atctgtaggt agtatgggga ttttggggag tgccgttgca ctgtttgctg cacaactgat
     1501 gccaagttca gccagttcga atggtctgtc tttagcgttt ttaggtggac tttaccttct
     1561 acgagcaata acagatgtag ctgattcctc cctttcgatg tggaatcctt taggctggtc
     1621 ttatttgacc tatccattta caactaacaa ctggattcct ttgctttatt tgtttttgtt
     1681 cagtctaatc ttgataatca gttcatttgt tttagaggaa catcgagatt tgaacaccgg
     1741 ctatatcccc gaacgtgaag gaaaaccgta tgctaaacgt tctcttttat ccttgcgtgg
     1801 tttattattc tatctaaata aagggatgac aatcagttgg cttatcactt ttggtttgat
     1861 gggggcggca tacggctcta tctacgggaa tatgcaacag tttttagaaa gtaacgactt
     1921 aatgaaacag atgtttatcc aatctggcgt ctcaattgaa aaaaacttta caagcacgat
     1981 catggtagtc ttgatttgtc tagctgctat tttaccaatt gctagcatca atcgtttatt
     2041 tacagaagaa acccacggac gtatgaatca gctgttcgca acaaaaattt ctaggaacca
     2101 actttattgg accaatatgt tactagctat tctatttgct atattggcaa tcctgattag
     2161 ttctggtgca ctagggcttt ctgctttggc ttcgatgaag gatgcgaaag acatggatct
     2221 attgatgttt cttaaagccg ggttcaattt cctgcctgcc gttttagtgt ttatcggaat
     2281 tagtggcttt ctattcggat ggcttcctcg aatcggtaaa cttgcttata tctacttagc
     2341 ttactccttt gcactcaatt actttggcgg aattttggat ttaccagaat ggttttccaa
     2401 aacagctgtc cctagctggg ttccacggat gccaaacgat tcttttgatc tttccgtgtt
     2461 ccttacgtta tcatgtatca gccttgcact gttcgtcctt ggttctattg gttataggcg
     2521 aagagactta ctagaaggat aataaaaaag catgtacaat ttcatgctag cctccaaatc
     2581 tgggtcaaaa atccaacatt tggaggtcat ttttttattt acaagtcttc taactgctct
     2641 ttatcaatga tccagacttc tcgattcttt ctttttacat aaccattctc ctctaataat
     2701 ttaaattttc tagataaagt ttcgggggtc gtaccgataa atgcagcaag ttctttcatt
     2761 ttcattggca ggaaaaagtg atccttccct gtcgctttag ctaagtcaag cagatagaga
     2821 gcgactcgtt cttctacttt ttccatcatc aaaaaagttg tctgctgctg tacttttgtt
     2881 aatttctcaa cgttgattgc taaaagtttc agactaaggg cgggataatg cagtaagagt
     2941 tccgagaaat ctttttgtct caatatgcaa atttccgttt tttctagtgc ttcgccaaat
     3001 aaagactcat ttggaacgcc tagcaactgg ctctctccct catatccgcc cggctctatt
     3061 actcgtaaaa gctgttcctt tccgctagca gataattgat acactttcat actgccggag
     3121 gccacgatca ctaattgagg ttctccattc ggcgcaagaa tctgttctcc tttttccaca
     3181 tagtgatgtt tgacaagtgc gtgtactttt tcttgatctt gctgtactaa gtgattgaac
     3241 aaaggaacaa atgaaacaca taaatgatca tgactcataa tctctcctcc ttctaaacag
     3301 ctatttctaa taaagaaaca taacttccct tattccaact gataattttc ttttatcata
     3361 gcataaacgt caagcgaacc agataccagc aaatagagtg gatttctttt tgtcccttgc
     3421 tttattttcc ttcttttgag accttaagta attgattcct atcaattttt taaatattta
     3481 ctttttgaat aatcaaaaac aaaccttcac tcgcaagccg agagtaaggt ttgtttttcg
     3541 cattgcttat ttcgtgttgt ctattgaaat ttttccgcac ctagcttttg atatagtggt
     3601 gctagttctt gtaaggtttc agcggttatt tgctcaacgt gttcgatttc ttgcggatag
     3661 aagaatagtt tacccatttg aaaggttcgg tctctcatcg cttttttcaa ctcttcttgt
     3721 ttctttgtat cggacaaata gtcagacaaa gctaagtggt cgtcaagttc atgttctggt
     3781 tgcggccata tgtaataatc cttgatatcg actgttttgc tccactcttt cagtatcgca
     3841 agcaactggt tatacgctgc aatgctacca tatcgttctt cgcttttata atgttggtac
     3901 atcaaataga tttggtattg tttttgattc aacaaaactg ctagacaggc attctcgtct
     3961 tgacgagatt cgcttcggta cgcgctccaa aaatgactgc gtaaattcca gccattcgtc
     4021 cagctttcga ttttaggttt gctcatgccg tacatatcag gcaaaagtgc agctgtctgg
     4081 agctgaatct ctttccattt ttgccagtgt tctttatatt ctttcttgat ttgttcaatc
     4141 ttatctgttg gatggctctc tttgagctgt ttgaatgcgt agattggtcg atcaaacaac
     4201 tgataatctg attttccaag ttccattgtt tcgcctcttt ccgttatctt ttgtattcta
     4261 cctgaatttt gcgatatgga caaatagagg ttgtgaaaaa gctgttctgc atcaagtcat
     4321 aagaacagcc aaacaaaaaa ctgcgatgag catcaccgca gtttt
//
LOCUS       DADVXU010000149         4347 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.156, whole genome shotgun sequence.
ACCESSION   DADVXU010000149 DADVXU010000000
VERSION     DADVXU010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.156"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            107..562
                     /locus_tag="KB953_002721"
                     /pseudo
     CDS             107..562
                     /locus_tag="KB953_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            659..2908
                     /locus_tag="KB953_002722"
     CDS             659..2908
                     /locus_tag="KB953_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBB6867631.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            2911..4086
                     /locus_tag="KB953_002723"
     CDS             2911..4086
                     /locus_tag="KB953_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB6867632.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRVTRGAS"
     gene            complement(4089..>4347)
                     /locus_tag="KB953_002724"
     CDS             complement(4089..>4347)
                     /locus_tag="KB953_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HBB6867633.1"
                     /translation="GITISVPPRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQN
                     FNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1514 a          633 c          791 g         1409 t
ORIGIN      
        1 gtaataatat aaacataata aaacaaaaat tatccaaatc taacatagga gggtggatat
       61 ggaaaattct atatatttag taacagaacc taacactcag ctgaaagatc aggacaatct
      121 agtcagtgaa ttgtccagac aaaaacaagt ttataaattt ttagaactcc tcttaaattc
      181 agctagttat ctgaacgaag ggataattac taaaaagatg aatccttttg ttcgtcaaac
      241 aattgaaata atacgtgaga atgcgtatca aaaattatct gttaaagata ttgctgtcag
      301 tctatcgata cacgaaaatt atttatcacg attgtttaag aatgatatgg gaatatcaat
      361 aaaagagtat attagtgaaa tcaaaatttc tattgcaaac gatctgttat catcaactga
      421 atattcaatc cagcaaatca gtgacaagat aggattctca acagtacaat ctttttctaa
      481 agcctttaag aaagcaaatg gtgtttcacc gggagtatat cgaaaacgta gattaggtct
      541 aggaatcatt aatggtgaat aaactaaaca tttgattttg ataaatattc gttactttaa
      601 acgcttacta tttatgtgag cgttttttat aatgcaataa agaaacggag gagagcttat
      661 gtataaggat aatgaaatga atattattta tcgatcagaa aaccaacaat ttcatttgtt
      721 taatcaggag ataagttaca tttttaagat atcggaagat ggtaaattat tgcatttata
      781 ttatggtgag aacttaccag ataatgacta tagtcatcta attgagatgc atcatcggcc
      841 tatgacaacc tatagaaatg aaaacgattt gctatattcg ttagagcact taaaacaaga
      901 atttcctgaa tatggtacaa cagattttag acatcctgct atttcattac tacagaaaaa
      961 tggctctcgc ttatcagatt ttgtctatca agggtacgaa gtggaagaag gaaaacctaa
     1021 actagaaggt ttacctgcta cctatgttga taataatgat gaaagcaaaa cattgaaagt
     1081 ttatatttta gattcagtta caaatgttca agtagaatta ttatatacaa tttatagaga
     1141 ttatcctgtt attactcgta gcgcgcgagt tatgaataaa ggaaaagata ttcaaataat
     1201 cgagaatatt agcagtttat cattggattt acccgatgca aattatgaat ggcaacaatt
     1261 atctggtgct tggggtagag aaagaactat taagtcgcgt actttacaac aaggaattca
     1321 atcaatcgaa tctactcgag gtatttcaag ccatcagcat aacccatttg ttactttaaa
     1381 aagaaaaaat actgatgaaa atcaaggaga agctattggt gcagcactag tttactcagg
     1441 aaacttctta attcaagcag aagtagatac atgggatgtt actagactac aagttggcat
     1501 caatccattt ggatttaatt ggaaactcaa tccaggagaa atatttacag caccagaagc
     1561 gattctggtt tattcaagta agggtttaaa tgccatgagt caaacttttc atcaactatt
     1621 tagaaaacgc ttagctcgtg gtaagtggcg agaaaaagac cgtcctgtct taattaataa
     1681 ttgggaagca acatattttg attttgacga agaaaagttg gtaaatattg ctaaaaaagc
     1741 atttgatgtt ggtattgaat tatttgtatt agatgatggc tggtttggag aaagagaaaa
     1801 cgattgtgca ggattaggag attggcatgt aaatcctaaa agactaccta gaggaatcgg
     1861 cagcctttca gaaaaaattc gtagttttgg tatgaaattt ggtttatggt ttgaacctga
     1921 aatggtgaat aaggatagtg agttatatcg cacacatccg aattggatta tttctacacc
     1981 taatcgaaaa cctaatcatg gtaggaacca atatgtatta aattttgggt tagatgaggt
     2041 tgttgaaaat attttcgatc aaatgtgtaa aatcattgat gaatcaaact tagattacat
     2101 taagtgggat atgaatcgtc cattgactga tgtatttgac agtcacttgt cagcagatca
     2161 acaaggagaa gtattccaca gatatgtact aggcgtatat cgcttatatg aaaaacttat
     2221 aacaaaatat cctaacatac tatttgagtc ttgttcatca ggtggtgggc gctttgatcc
     2281 tggaatgctt cactatgcac ctcaagcttg ggctagtgat gattcagatg caattgaaag
     2341 gttaaaaatt caatatggaa ctagtatgct gtatcctctt tcgtctattg gtgctcatgt
     2401 atcaattgtt ccaaatcatc aaacaaaccg tataacacct ataaaaacta gaggaaatgt
     2461 agcttttttt ggtgcatttg gatatgagct tgacttaaat gaattaagtg aaggcgattt
     2521 gaggattgta aaagaacaaa ttgcctttta taaaaaatat agaaccataa ttcataatgg
     2581 tactttttat agactattat caccattcga tgatgataat caaaccgcgt ggatggttgt
     2641 tagtcatgat aagaaaacgg ctattgttgc tcattataaa acattaaatg aggtaaatgc
     2701 tccttaccgc cgactgaaat taaaagggtt agataatgac ggactttata atgtaggaca
     2761 aaatagtttg cgatctggaa tggaattgat gagagcagga ctagtctgtt cagatgcatc
     2821 tagtggacaa ataaatgatc agaatcgacg cccagaaaca tttgattttg attccaagat
     2881 ttgggttata acacaggagg aaaagtagag atgaataacc aaaaaagctt acttcaaaaa
     2941 ggagcaatct taagtatttc actaatgtta acaagctctc aagcaattaa tggtgttatt
     3001 ccacaaatga aacagtcttt ggggatatca caaagtcaaa gtgaactttt aggaactgca
     3061 ccaagtataa ctgttatttt gtttatctta ttatctagtt actttgccaa aagaatagga
     3121 atgaaaaaga caataatttt aggtttatta ttagctggta taggaggaat tattccagta
     3181 tttacagcta ccttccaaac cgttcttatt agtcgagtaa tattaggtgc cggtttagga
     3241 gtatataatt cattggctgt gacatatatc agctctttat actctggcaa tactagagca
     3301 acgctattag gtattagaaa ttcaatggaa gctttaggtc agacaatcct tatttttctt
     3361 gctggagttt tagtgaacat taattggacg gcttcgtttt tagtctatgc aattgcgttt
     3421 cctgtagctc tcttattcgc gctaaaagtt cctgaaatta gagatgaaaa tagtgacata
     3481 cctgaaaatc atgtaaaaga aaagatgaat ccaatggttt ttgccctagt tctatttgca
     3541 atcttactcg taatgaatag tattgctatt tctgtacgat ttgcatcaat tgcaactgaa
     3601 attaaagggg taaactttaa tgctagtaac tatttagctc taatgcctat cttaggaata
     3661 ctagcaggat ttatgtttgg accaataaat aagtggatag gaaaaggaac cctatacttg
     3721 ggaattatat tctttataat ttcaaatttg ttaattgctg tgtcaaacgg taatatgacg
     3781 ttcctgctaa cgggtttatt tttatcaagt ataacaggat catggtgctt cccatttatc
     3841 tttagtaact tagataaagt cactacaaag aatacaatta attttgctac ttcattgatt
     3901 tttattggct gtaatattgg taattttata gctcctattg ctatgcaatt agcacaattt
     3961 ctaacaagaa gtacaaattt gactgctcca ttttttgtat tcgcaggaat atttattgtg
     4021 atcctatttg taacattttt tgcgacaaat aaaaaaaata ctagagtaac ccgtggtgct
     4081 agttaatttt aatatcctaa agaatatctc aaagttcctt caaaacgtcg ttgtgctcga
     4141 cttaatagaa cactgtaagc cagtaatatg ctttcaaatc tactctctag ccctttaaca
     4201 gaacgtgagt tgaaattttg acaacctaat ttttctaagg aagaaaaaac agtctcaact
     4261 gtttttcttt gttttccaag taagctgtgg tctagttttt ctgatttatc catgttttta
     4321 cgtggcggaa cggatatagt aatgccc
//
LOCUS       DADVXU010000150         4249 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.157, whole genome shotgun sequence.
ACCESSION   DADVXU010000150 DADVXU010000000
VERSION     DADVXU010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.157"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            111..1412
                     /locus_tag="KB953_002725"
     CDS             111..1412
                     /locus_tag="KB953_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBB6867634.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1439..1699
                     /locus_tag="KB953_002726"
     CDS             1439..1699
                     /locus_tag="KB953_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBB6867635.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2698..3933)
                     /locus_tag="KB953_002727"
     CDS             complement(2698..3933)
                     /locus_tag="KB953_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBB6867636.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1234 a          676 c          878 g         1461 t
ORIGIN      
        1 ttaagagaat atattgaaat aaaatttctt aattatataa ttagttatga acattctttg
       61 cgttgtcatg caatgaatgg ttcatatttt attgaaaagg agtggtttta gtggaaaagc
      121 tgatgaaatg ggtcgaagag aaacttgtcc caccaatggc aaaaattggt acacagcgtc
      181 atttattagc aattcgtaat ggggttgttt caacattgtc attgattttg attgggacat
      241 tttttatggt ctttatcaat ttaccgtttc ctggatggaa tgaatttatt gccccttatt
      301 cggcaacgat tgttttaccc tttaggatga cgatgggatt gatggctatt tatgcaactt
      361 ttgttatggg ttcggatcta gctaaatcat atggattaga tagtgtgaca ggtggtatat
      421 tatctttagg aacgttcttt atgttacaag ttcctgtaaa cgttttaact cctgaagagg
      481 cacctcttgg ctgggtctta cctatgagtt ctttgggagc atctgggatg tttgcgggga
      541 tcttatctat gatttttgca gtggaagtat atcgtttctt taaaaatcga aatattacta
      601 tcaaaatgcc agaacaagtg ccacctgctg ttgcccgtag ttttgaggca ttgattccag
      661 gtgcagtcat tttaacaatt acttggctga ttcgaagcgt cgcgggcttt gatgtaaacg
      721 ctgccttatt atcgttattc gagcctttaa ccaatatttt agggaataat ttgcttggtg
      781 ttttactacc aatgtttttg atccatttgt tgtggtcatt tggtatccat ggcatgagta
      841 ttgtaggggc tgttgttcgt ccaatgtggc tgatcatgtt ggatgagaat gcaaaggcat
      901 tggcagatgg tacagctgcc acccaattac cctatatcgc tcctgaacag ttttatcaat
      961 ggacggtaac aattggtggt gcaggagcaa cgatcgtggt atctgttttg tttatattct
     1021 tctgtaaatc aaaattcttg aaagaggttg gtcgtttttc tattattcct ggtatcttca
     1081 atatcaatga gcccataatt tttggagctc ctatgatgtt gaatccttac atgttcatcc
     1141 cgttcaactt agttccttta gttttgacta ttatcagtta ttttgcagtc aaattagaaa
     1201 tggtcaatag atttactgtt ttgccaacat ggacattacc agctccaatc ggcggttatc
     1261 tttcagcagg aaatgactgg cgtgttgtta tcttgatcat gatcaacaca ttgatagcat
     1321 ttattatcta ttatccattt gtaaaagctt atgataaaaa gatgctgtca gatgaacaaa
     1381 gtcgtggaga aaacgaagtc cagactgtct aagtttggtt tttagaaggt gggaaacaat
     1441 gtattggctg attttatttt ttgtttttat ctttttatta acggctagtc accttatatt
     1501 gaatatgcta gctacatatc atattcaaat caatcgatgg atttgggcac tggcatcatt
     1561 tttgatcgtg attcttccaa agatcattgt accgcatatg aatgttttgt tttcatgggg
     1621 gacatatgta ctttgtggaa tttttgctat caattttatg atcgagcagc atagatggtt
     1681 tgtcacatcg aagctttaaa tgttctaaaa gtggttgtaa cagaagtggt tagcttcaag
     1741 aaataagaag aaatttacga aaattgcttc ttaaattttt gtgaaatgtt ggcttatttt
     1801 tcgtttggtt gaacttatta ccgcagatgc aagcttttga ccaccgttta ttcggaatat
     1861 gggactgtga caaggctttt gtcacagccc ttttttacat aaagattatt attgttttaa
     1921 ctgttttggc agaaagaagg aggaaaaaat ggaaatttta tcagcaatgg atttagaatt
     1981 agtagatcta ctaccaaagt gatcaagtca aatatagatt tgggagctta caaccatata
     2041 gtaggggctg cagtatggtg tatgaatggt aatatgtatg atgtgagtgt ttattcaaat
     2101 catgaaacgt gtatagaaat aattgtgctg ggaatagcaa tcagtcatgg tgaacgtgac
     2161 ttttagtgta tcgttgctga cgacatagaa cattctgaca tgatctactc aatatgtggg
     2221 gattgctggc agtttatata ggagtatgca tcggattgcg agatcattgt ctcaacagaa
     2281 gagggaggtt gattatcaaa gataaagtgg taaatcctac atttgattta gtaattgttg
     2341 aggggactag cggcacaaat ctctacaaag cgatgcttct agatctatat gacacatttt
     2401 aagaaaatgt atttgcctaa ttattatggt ctttcttttg aagaaacgtt gagatgatcc
     2461 aaccagagaa aggtgtcaaa gaatttgact aagaataaat ggcagacgct acctatgctc
     2521 atttggatag cgtcagatct tttattttaa ggatgatttt gtgtttttta gacaccgtaa
     2581 tttaagtaac tctaactttt caatccacta tttacttttt ttgtattttc actgtctggg
     2641 tctcgaatag cgaaattact gatgagtctc tattaaaaaa gctttttcta cttgaaacta
     2701 tattgggtgg ttgctcagga aggtttcgat ttcttgaatt gcttgttgtg gaccatttat
     2761 tggaaaaacg gcttgcagcg tgcgtgcctg ctgacgaatc ttgggtgaag aaacttcgcg
     2821 cagagcaatc tgcagttttc tttcagtcac tggttttgtg agggcgattg cgtttccgta
     2881 ttttacacaa tagtcaatat tacagctttg ctcaaattgc atccccacac cgataaacgg
     2941 tttgccggat tcacaggcag tttggacggt cccttccccg ccgtggataa aagaaaaatc
     3001 aatgaattca tgcaaaagat gactagacag atattctgtt attaaaagat cttctgagtc
     3061 agattgaatg tcatcaagca gatagtcaat aggtgcaata accgctagat ccggttgttt
     3121 ttgtaagaag aacaaaattt tgcgtaacat acgcggattg cctgaactac ccattgcgaa
     3181 atagataata cgttttcttt cttgttgctt tcgctgtata aaagttagca cttcttcagg
     3241 caaggaagta ctgagcttag caaaaattgg accaacgatt cgataatttt ccggcagctg
     3301 ttcttcttcc aaaaatagtt taggcgttgt gatgagattt agatcagcat ccatcatttg
     3361 taaacttgtt tttggtaaag gaagcatttc tttttgtgca gcgtgtttga aggcattggg
     3421 gatccatcgg gtatttacta tacgcttttt tgtccagtgc caaaggggat tgcttaaagg
     3481 gccgaagaaa cgtaactcgt ggggcatcag cgcatgctct gattgaaaaa aaggttcact
     3541 caaagcatag ggtttaacat atactaaagg aatcttttta ttgcgggccg ataaaaaaat
     3601 tgtcacattc gaacctgtca caatcagtcg aggctcaaac ttagcaatca gactctgttc
     3661 actagtgata cgagaggcaa ccatttcttt ggtaaaaggg tttttaagtg tacgcaactg
     3721 gtcaaatttt atgatttgtt cttcttgttt tttcgtaagg atcggcgcta gtaaatgata
     3781 ctcaaagcca ctttctgtga tcaaaggagc aaaagtttta gaaaagccaa agaatacaca
     3841 gtaataacgg tctttcatag ctttggcaac ctcaatcatt cgtgttgtct cagctaaatt
     3901 gaatactgca ggagcaaaaa gaattgtctt catgaaatcc tccttattgg aatgagtcta
     3961 tcccttcgtt tcttactagt atactaaaaa acacagcgtt cgagtagcct atgtatattt
     4021 tgaatattaa actgtctctc gctgttttca caacttctaa atttaaattt tgtaatcaag
     4081 ttgaaactcc aattttcaaa aaaacacgca acaatttttt gatcgattta tatgttgtgc
     4141 cagataaata cgagaagttg tgtcattaaa aataaacatg agaacataaa aaaataaaat
     4201 aattatatta attgataggt gtgctgtcgt cattatacac acgtaagaa
//
LOCUS       DADVXU010000151         4199 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.158, whole genome shotgun sequence.
ACCESSION   DADVXU010000151 DADVXU010000000
VERSION     DADVXU010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.158"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            127..579
                     /locus_tag="KB953_002728"
     CDS             127..579
                     /locus_tag="KB953_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867637.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="KB953_002729"
     CDS             593..1282
                     /locus_tag="KB953_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867638.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="KB953_002730"
     CDS             1407..3758
                     /locus_tag="KB953_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HBB6867639.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..>4199
                     /locus_tag="KB953_002731"
     CDS             3838..>4199
                     /locus_tag="KB953_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867640.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNP"
BASE COUNT         1458 a          541 c          846 g         1354 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccc
//
LOCUS       DADVXU010000152         4105 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.159, whole genome shotgun sequence.
ACCESSION   DADVXU010000152 DADVXU010000000
VERSION     DADVXU010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.159"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(214..1719)
                     /locus_tag="KB953_002732"
     CDS             complement(214..1719)
                     /locus_tag="KB953_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBB6867641.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDPFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1716..2402)
                     /locus_tag="KB953_002733"
     CDS             complement(1716..2402)
                     /locus_tag="KB953_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBB6867642.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2595..4016)
                     /gene="gndA"
                     /locus_tag="KB953_002734"
     CDS             complement(2595..4016)
                     /gene="gndA"
                     /locus_tag="KB953_002734"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HBB6867643.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1129 a          864 c          696 g         1416 t
ORIGIN      
        1 tccctatttt tatttattat ttttaaacac gaatattaca gcattttatt gttttttcgt
       61 tcatctaaaa gatacttaca tttaattaac ggatgttatt ctatttaatt gttcgctagt
      121 ttttttataa tgacatccct gaatttctca cttgcttgta ttttgtacct tattctttgt
      181 tggatatata aaaaaacaag taaagcacat gctttacttg tcttctgcct ttgcgatagg
      241 aataaagatt ctaaaaattg tcccttgccc tactacactt tctgctaaga tacgtccttt
      301 atagttttca accagttgtt tagcgataga caaccccaga ccatttccac ccttcgttct
      361 agctcttgct ttatccactc gataaaaacg attgaagatc ttattaaggt cttctttggg
      421 tattccttcg ccaaagtcct gaatcgcgat ctcaaattca ttcagagtgg aagaaataga
      481 gatatggact tcttttcgtg tagttgaata ctttactgcg ttatccaata tgataatgat
      541 taattgttca aagtgattac gataaatttg cagggtcact tcttttgtca aatcatcatc
      601 taaagtgatc acatagtctg gatataatat cttgaaatta ttataaactt gataggtgac
      661 ttcttttcct ttagttgttt cattcgcata atgaacatct acttgttctg ctcttgataa
      721 gtctagcatc tcttgaacta agcttttcat acggctgatt tcttgcatgc tggcgcttaa
      781 tgattcttct aatatctcag gatcatcttt cccccaacga tttagcagac taagatgccc
      841 ttcaataatt gcaacaggtg tccttaactc atgagatacg tcttccacga attgctcttg
      901 ttgttcaata tacaaacgca tacggtcaag catctcattg aatatttcag ctaaatctgc
      961 caattcatca tttgtgtcga tttcaggcat gtggatatct gattgcggat ctttacgtat
     1021 agtatccatt gtatctctga gtaccttgag tggttttaag aaataagaag ataaaaagaa
     1081 acctaaaata ctacttaaga tcaaagaaac aacttctaaa acaataagag tcaataatag
     1141 cttatttcga atatcataga aagaagataa ttcatagaag ctttgcgcat agccgatctt
     1201 ttctcttgta cctttagaat atataggctg gatagataaa aagccagttt ttccttctag
     1261 tgtaacgatc gttggaactt tacgtgtagt ctgaatcagg tttcgactgt tttcatgggt
     1321 tttaaaaatc aagttctcgt ccaaatcata aacataaaga tccattgatg gctgccctag
     1381 ctcagctata aagggatcga tcttcattaa gtttccttcg accagtgaag tatgatctac
     1441 gacctcatct ccgtcatttg tcgctttcgt cagattacga tacgtattta ctaatgtcag
     1501 ttcttcatcc gagttagcca aacgagaagc tacttctgaa atcgtacgct ctacattgtt
     1561 tctttctttt gccacaatca gattgataga tgatttatag gtaatgacag cgaaaattgt
     1621 aaacacaacg aatatgaaga aagaactcgc aaaggcccac ttgattgtca aggagggccc
     1681 tttcagttct cttttttccg attttttcgg ctttatcacg agcgcatcac ataacctgtt
     1741 ccgcgtacag tttggatata gctttcctcc ccgggaacgt caattttgtt gcgaagataa
     1801 cgaatatata cgtccactac gtttgtttca acttctgttt cgtatcccca cactttattt
     1861 agtaaaacgt cacgtgctaa taccacgttc acgttttcca ttaaagtaag cagcaactcg
     1921 tattctcttt ttgttagttc aattacttca tttccgcgac gtacgacacg gttttctttt
     1981 tcgatagtca aatcgcgata agtaatagta gtttgtttag ccacattttt atcgccttca
     2041 atatcgattc gacgcaacaa tgcacgaaga cgtgctaata attcttcgat agcaaatggt
     2101 ttgacgatgt agtcatctgc tccgtggtca agtccagata cacgatcgat cacagaatct
     2161 cttgcagtca tcataatgat tggtgtattt ttgacttggc gtacacgtct gcagacttct
     2221 aaaccgttca attctgggag catcagatcc aacaatatgg catcccactc attatttaat
     2281 gccgcatcta aaccagttcg gccgttgtag tggacttctg cgttataccc ttcatgcttc
     2341 aactctagct cgacaaatcg tgccaagttc ttttcatctt caatgattag aatattgctc
     2401 atttattatt ttatcctttc tattctactg gctttttaaa aactctatca ttataccgat
     2461 aatatgctta aaaatctata gtcattttat catggatagc aattttaaaa aagagctgaa
     2521 gtctactcat aacaatcgac ttcagctctt tcgtcctctt tttattttct catttcctaa
     2581 cagccgaaag actattattc ttcatggtac catgagtaat ggaagattcc ttctttgtcg
     2641 acacgctcat aggtatgtgc gccaaagtaa tcgcgttggg cttggattat atttgcaggc
     2701 agacgttctg ctctgtatga atcgaagtaa gcgatggctg atgagaaagt cggtacaggt
     2761 acgccagctt ggacagccaa ggcaacgacg tctctgactg attgctgata ttttttcgtg
     2821 atttcaacga agtaatcatc tagcaataaa ttttcgatct caggattttt gtcataggca
     2881 tccgtaattt tttgcaagaa tcgagctcgg atgatacatc cagcgcgcca gatttttgcg
     2941 atttcaccaa atggcaagtc ccaatcaaat tcttttgaag cgacacgtaa ttgagcaaat
     3001 ccttgggcat aactcatgat tttactgaaa tacaacgctt ctcggatttt ttcgaccaat
     3061 tcttttttgt ctcccgtgaa ctcaaaatcg ttcgtacgag acaaaatctt gcttgcttgt
     3121 acgcgttctt ctttataagc agaaatatag cgggcaaata cagattcagt gatcaatgga
     3181 agtggcacac ctaagtcaag cgcgctttgg cttgtccatt tacctgttcc tttattccca
     3241 gctgcatcaa ggatcacatc tactactggt ttacctgttc cttcatcgtc tttacgtgtc
     3301 agaatgtcag cagtgatttc tatcaaatag ctgtctaatt cgccttgatt ccattcttta
     3361 aagatatcag ccatttcttc tactgaaagg tttaaaatat tcttcattaa gtcataagat
     3421 tcagcaatca gttgcatatc tccatactca ataccgttgt gtaccatttt cacataatgt
     3481 ccggctccat ttgggccaat atatgtgaca catggtgctc cgtcttctgc ttttgcagaa
     3541 atttgctcta agattggtgc aactagcgca taggcttctt tttgtccacc aggcatgatt
     3601 gaaggtcctt ttaaagctcc ttcttcacca ccggaaacgc ctgtaccgat aaagttgatg
     3661 ccagagttgg ctaactcttc attacgacgc atcgtatctt taaagaatgt atttcctccg
     3721 tcaatcaaaa tatcgccttt atcaaggtga ggaagtaact cttggattgt tgcatctgtt
     3781 gccggtcctg ctttgaccat tagcaagatc cgacgtggtt tctcgataga ttctacaaat
     3841 tcttcgattg tatatgttgc tttgaaattt ttatctgggt gttcttctac tacagcggtt
     3901 gttttagccc ctgtacgatt gaataaagcc actgaatagc cgcggctttc tatgttcaag
     3961 gctagatttt tacccataac ggccataccg acaacgccga tttgttgttt tgacatatga
     4021 actcctccta catacgggat ttctccctaa tctcttacat tatagcttaa cacggctaca
     4081 ttgcaaacta aagaacaaca tattt
//
LOCUS       DADVXU010000153         4074 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.160, whole genome shotgun sequence.
ACCESSION   DADVXU010000153 DADVXU010000000
VERSION     DADVXU010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.160"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            526..2019
                     /locus_tag="KB953_002735"
     CDS             526..2019
                     /locus_tag="KB953_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HBB6867644.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            2154..2450
                     /locus_tag="KB953_002736"
     CDS             2154..2450
                     /locus_tag="KB953_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HBB6867645.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            2551..3402
                     /locus_tag="KB953_002737"
     CDS             2551..3402
                     /locus_tag="KB953_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867646.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            3423..3706
                     /locus_tag="KB953_002738"
                     /pseudo
     CDS             3423..3706
                     /locus_tag="KB953_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1452 a          586 c          824 g         1212 t
ORIGIN      
        1 taagaattca gactttataa ggaatatagt ggatatacta aatatatttg ctatattcag
       61 tatatcagat atattcaaaa tgtcaaaata tatttgtgcg ttttgcgttg acaatttaga
      121 gtgaaaagag gtaaacttaa ttctatagaa accacagata attcactatt tacaaagtag
      181 caaaagcagt ggtactcttt aaacagaaaa attacataaa aaaaagagac ggaaataatt
      241 gattttagca gaatcaatta tttctgaatc acatggttag gacatgtgtt cacgctcgtc
      301 tcaagaatat gtaagtagct accaactact tatattaaaa gttgatcgga taccttttcc
      361 gattgccttt attctaccaa aaaaaggcga gcaactcaac agaagaacgt ttatttagtg
      421 tacttaaatt aaatattctt aaacactgaa gagagaacac atactatggc tgagattaaa
      481 gctaaactgt gttctctttt tgtttgagaa aggacgttac aatcgatgtt gagcgaaatt
      541 tattcagcta ttcttaaaaa cggcttacgt acatacaaat atcgaaacag caccatgaaa
      601 cctgcaggtc atattgattc cagtaggagt ggtgctatct ttgcgtaccg tagcaagcaa
      661 ttaatgaatg ttgggcgtgg tatggtcatt acttcggaag aagctatttt agagaacgaa
      721 aagaaactaa cgcattggac acctaatacc tatcgctttg gtacttatgc ggatgattat
      781 cgaacagtgg ttaaaggtca ttctgagaaa aacttaagtc aaatcaacac atttgtagtt
      841 gatatcgatt caaaagagaa ccatcaaggc gaaattattt tagcctgttt agatcaagtg
      901 ggctacatgc caacgctaat tttagaatca gatcatggtt atcaagttta ttttgtacta
      961 aagacaccag gctatgtcac aaagaaaagc aatttcaagg tgatcgatgt agcaaaaaaa
     1021 atttcaaaaa caattcgtct acagttggct gaaaaattgt ccggagtgga tctaggatgt
     1081 aaccacttcg gaattgctcg attcccacaa gaaaataata ttgtttttaa agaaatgaac
     1141 tatcagtatt cttttgctga ctggattcaa tggtcgatga agcttgaatc agatcaaaaa
     1201 acaaaagaag atcgagaacg aaaactaatt gtctttcctg agaaaaaaga aattcgtcaa
     1261 gtagatgaac cgtggttcga tatgttactt cataaagcaa acatcgtcgg cggagaagga
     1321 cgtcttggtc gaaataatgt gattttcacg ctttctttag cttattactc ttcaggttat
     1381 gcattggata cttgcgaata caatatgttc acattcaacg aacggttaca ggaaccgcta
     1441 tctgaagcag aattagtaaa gattgtacgt agtgcttatt ctggtaatta tcaagcagca
     1501 agcagagaat tcattattga attgtgccag caatgggttt caccaacctt aaaaaataat
     1561 gatctgttca ttcaacgacg tggctggtgg aaatttaaaa agccacgaga tcaaagaaaa
     1621 tatagtcata aaaatgaatg gaaagaagat ctattggctt atctaagcga aaaaagtctt
     1681 gctagtgagc cttatttgaa cctgtcaaaa aaagaattgg cttcacaact aacgatgccc
     1741 aagcgaacac ttgataagat cttaaatgag ttacgagagg agaataaagt cttctacaag
     1801 gtaacacgag gaagaaatgg cggaatgatc attgcttcag tcaaaacgtt gttcgcgagg
     1861 gttattcaac taagtaaaga aaagagacta gattattcaa caaggatcac agaggtattt
     1921 ggactcaacg agagcttagt agaagagaca tttagacaat tatcaacccc cttgaaccct
     1981 cttaaagaag tgaatctttt tgaagtagat acaggttgaa aaataaaagg tgcaaattgc
     2041 cttatacaat atatctatga tacttctgta ggtgttgctc cctttgccta gctacgtgta
     2101 ggtggtatac ttataactgc gtatgcagtc ttatgtatca tggaggaagt tgtatgtcgt
     2161 taaaaaatta tgtctattca catcctgtta atgtgtttat cattggtcgt ttgggattat
     2221 ctgtggaagt gttctgtgag ctttatggtt ttaagcaggg gactttgtct tcttgggtta
     2281 ccagggaaaa aacagttgct tctttgccga ttgagtttct tcatgggttg tccttagctt
     2341 ctggggagac tatggatcag gtatatgatt gtttgtgtgt gttagagcag gagtatattg
     2401 agtataaaat agctaacgag ttaagaaaaa ggaagaagta tatccagtag tagtttttat
     2461 ttacttaaaa aattggttca ttgagcaacc tatcgctgct aaagtagcaa aggaaaacgt
     2521 taaaggaaag aatttaggga agaaatcgaa atgaaatcgg acgactggat tgaacacacc
     2581 tggcttcctc tgaaaagaaa tagcaaacga aagaaatttt gggtattgca attatggcca
     2641 ttaggaaaac tttgtagtta cggggcacga cttattcttg atttaaccgt ttactcgatt
     2701 ggagtatttg gaacggctta ttacttgata aatgatccca ataggattgc agcatcagac
     2761 atttttacag tgctaatgac gttgattttt gtcatgctat tgtctatttt tactcaatta
     2821 gtcatgcaat ttaccaggca aaaattaaca gcaggaaaac ttttcaaaag cctattcgat
     2881 ttaattgttg taaatgcaat tctagcgact ttaataaaac aaattgaaca atctggaaga
     2941 acaacaaaag gagtgaagga ttttttattg acgtttattc actttgtaaa tggacacatg
     3001 tatttgtttg aagtgggtat tattctcttt tttgtcgggt atgtatccac gaaacttatt
     3061 aaacgttcaa ttgaaaaaga aattgaaatt gacctgattc cgacacttct tggttatgag
     3121 tacaatgttg attttggaag atatgacgaa ttgatcaata cggatatcgt tcacacgagt
     3181 atccatgaat ctactgttaa gggcatcaga tctattttaa aaaatggcca ggaaatgaaa
     3241 gcaatagatc gaagaaaaga gatctacaat ttttatgaag tcgaagcaag cctacgatat
     3301 ggcatcggaa aattgtggtt agatggaagt aatattcgtc aaaacaaacc atacattttt
     3361 tggaacagta gtgatttaga acttccaatg aaagataaat aattatggag aatcgtttga
     3421 aaatgattcc tctatacagg attgtgctta tagttggcat ggagttaaaa accttaaaga
     3481 aggagcagtt attcaaagtg aaagaacgtc ataatcagtt actatgtagt aaaacgggta
     3541 cgatgaacat tgaataccga acagaatatt tagaattaac gaatcaaact gattttttgt
     3601 tgattcgaaa gcaacaagag caagaaaaaa attaatggct acttatagcc aattgattga
     3661 tgaatgggaa caattcaagg aatctaaaat tccagaacgt ctctaaaagc ctttatttgt
     3721 aaagagcata aggaaacgtg ggaacacttt ttaaaaaaag tgttccaata ggctgtgcct
     3781 attacgtttc tcaaaaataa atgtgggaac aaaatattta aaaataggat agtgaaagga
     3841 tagtgaagga tagtaaaagg agggtagtag gatagcaaaa aggataggct ccttttcgct
     3901 atccttttta aattggagga attaatatgg aagtaagaat aagaaatgtg gatgagaaaa
     3961 tcgtaagaga aattgatcga agatgtgacg aacggttctg ttgcaaagtt ttaaatctac
     4021 tatcaaataa ggtagaataa tagaaaaaga tagcaggagg aatgacgatg aatc
//
LOCUS       DADVXU010000154         4070 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.161, whole genome shotgun sequence.
ACCESSION   DADVXU010000154 DADVXU010000000
VERSION     DADVXU010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.161"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            86..1435
                     /locus_tag="KB953_002739"
     CDS             86..1435
                     /locus_tag="KB953_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBB6867647.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1563..2153)
                     /locus_tag="KB953_002740"
     CDS             complement(1563..2153)
                     /locus_tag="KB953_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HBB6867648.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2300..3652
                     /gene="brnQ"
                     /locus_tag="KB953_002741"
     CDS             2300..3652
                     /gene="brnQ"
                     /locus_tag="KB953_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBB6867649.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1176 a          744 c          789 g         1361 t
ORIGIN      
        1 aaaattttgg aatcttagtt gtgtttttca gaaaactctc ttaatatgga agtaattaaa
       61 cttttttagt taggatgatt taaaaatgaa tgaagagcaa gaactgcagc gtggactgaa
      121 aaatagacat gtccaactga tttctattgg cggagcgatt ggaacggggt tgtttttagg
      181 ttccggaaag tctatccagc tagctggccc ttcgatattg ctcgcctatc ttattactgg
      241 atgcatctgt tttttcatta tgcgtgcgtt aggcgaactt ctgttatcaa acaccaataa
      301 ccattctttt ctggattttg ttgcagaata tcttggcaaa aaagcggctt tcatcactgg
      361 ctggacgtat tggttctgct gggtatcgat tgcaatggcc gatttaacag ccatcggaat
      421 gtatgtacgc tactgggcgc ctggtgttcc tcagtggctt cctgaactga ttgcattagg
      481 tcttcttctt tgcttgaata tggtggctgt ctcattgttt ggtgagttag agttttggtt
      541 tgcattgatc aaagttgtag cgatcattgc ctttatcctc gtaggagctt atatgatcct
      601 cacccactat acgacagata ttggcacagc cagtatcacg aatctatggt ctcatggcgg
      661 attctttcca actggtgcaa aaggatttat cttagctttc caaatggtaa cctttgcttt
      721 tgctgggata gaattagttg gattagtcgc tggagaaaca gaaaatcctg aaaaagtgtt
      781 gccggaagct atcaataata ttcctatcag gatcatctta ttttacttag gatctctttt
      841 tgtcattatg gcaatctatc catggaacag cttgaatcca gataacagtc catttgtaga
      901 agtattttcg gaaattggga tcacgattgc tgcttccttg atcaatttag tcgtattaag
      961 tgcagcagct tctgcctgca acagtgcaat atatagtaca ggacgtatgc ttcgttcttt
     1021 agcgcaagaa ggcagtgccc ctaaaaaatt caaaaagtta acaacacatc atgtgccagg
     1081 gaatgctttg acattttcta ctatcgtcat tttcatttct gttatcttga attacgtgat
     1141 gcctagtgaa gtattcacac tagtaagcag tattgccact acttgtttct tgtttatctg
     1201 gagtatgctt gtctacacgc acatgaaata tcgtaaaagt atcctcggca aaaaagctca
     1261 ttcatttaaa atgccgttgt acccatttag taattatctg gtatttcttt acatggcctt
     1321 tgtgtgtgtc gtcttgttcc taggaaaaga tacgagaatc gctttattac taacacctgt
     1381 gtggttcttg cttctattac taatctatca tatgaaatat gaacgtaaga agtaagaaag
     1441 tagttgtgac agaagtggtc agcttcgaga aataagaagg aatttacgaa aattgcttct
     1501 taaatttttg tgaaatttcg gcttatttct tggagataaa agcttctgtc ccagcctctt
     1561 ttctatttcc ccggaaacaa ccgctgataa taggcatctg cttttttata ggaaaaaatc
     1621 caaataccag atacaaaaaa acgtccgaat aacccgatca acggtacaaa aaagacaccg
     1681 gcaatcaagc ataagttaat catcatggag ataagacttc gacggtcctt tgacaaaact
     1741 ctcacgacat gggaatctgg tttatttgct ttcatgattt gcctcatcaa gataccaaaa
     1801 gaaaaactcc acatcgtgta tactaagaca taagccatct caggccaaaa agcttcaggg
     1861 ttacgcccga cccaactagt aacagaagga cataaagtca gccaaaagag caaaaagata
     1921 ttcgaccaga ccactcggta attaattcgt tctgccatct gaaatatacg atgatggttc
     1981 acccagatca ccgcaacaaa tataaagctg atggtataag ctaataatgt atttcggatg
     2041 acccataaag aatgaaagct agatgtatta gggatcttga tctctaatac aagaactctc
     2101 aaaacaatgg ctaccactcc atcagtaaat gcttctaatc tcgactttgt cacgatgatt
     2161 cctcctcaga tgattcagcg cttttatttt atcacttacg ttattaaata accatcgttt
     2221 tatataggga attttctgaa aattgttgca tatttttcga aattttgtta cttttaaaaa
     2281 aaccgtaaag gagttatgta tgacaaaaaa attatcattt aaagactatc tttttatagg
     2341 ctccatgctt ttcggtctat tttttggtgc tggaaatctt atctttcccg ttcatttggg
     2401 tcaagaagca ggcgctgcta ccttttgggc gaatctaggt ttcttggtta ccggtatcgg
     2461 tcttcccttc ttaggggtga ttgccattgg tgtatcgaaa agctcaggtg tatatgaatt
     2521 agcaaaacgg ataaatcaaa catatgcgat ggtttttacc atcttgcttt acttagttat
     2581 cggaccgttt tttgctttac ctcgccttgc aaccacatct ttcgagattg gtttagcacc
     2641 atttatcgat cctagcagac agacgctata tctagcaatt ttcagtattg tattttttgt
     2701 gctggcttgg tggttttcaa gaaaaccgac aaagatatta gattatgttg gaaagttcct
     2761 gaatcctgca tttttggttt tattaggtgc tttgcttctc cttgcttttt tgaatccact
     2821 aggttctgta aatcaagcgc ctattcagcc aaattatcaa gagcatgctt ttttcacagg
     2881 gtttactcaa ggatataaca cattagatgc gcttgctgct ttagcatttg ggatcatcat
     2941 tgtcacaaca atccaaaaca tgggtgtaac aaaacctgct gaaatcgcta aagacacgat
     3001 caaatctggt gcgatcagca ttatattgat gggaatcatt tatactttgc tcgcttattt
     3061 aggtgctatg agcttaggta gctttgctct aagtgaaaat ggcgggatca cgctggctca
     3121 gattgctgat cattatttag gtacttatgg cagtatctta ttagcattta tcgttatatt
     3181 agcttgcttg aagaccggta ttggtttgat tacagctttt gcagaaacat tcacagattt
     3241 gtttccgcaa agaaattacg cattctttgt tgcacttgct agtatccttc cttgtttagc
     3301 ggcaaacgta ggcttgacga atatcattca attttcgttg cctgttttga tgttcatcta
     3361 tccattagca atgacactga ttttactagt gttagttggt cctttgttta agcagcgtac
     3421 ggctgtttat cgaatgacta cttattttac attgattgct tcgatttttg atgggttgaa
     3481 cgcttgccca gaaactatca aacaaacacc tattgttcaa aatcttttat atgttgcgga
     3541 aagctacttg ccgtttttca aattggggat ggggtggatc gtaccagcag ttatcggttt
     3601 tgttatcgga ctgatttgga gtttcgctaa gaaagaagaa gtagcagatt gataaaagag
     3661 gttgtgaaag agacgttcag ccttgagcaa taaaaagtaa ttttgaaaaa tggcttctca
     3721 catttttgca taaatttgac ttaataccga gaaactgact tttgaacacc gtttattgag
     3781 aggtcgtgaa aaagccgctt agtttcaagt attaagaaat attttgtgaa aatggcttct
     3841 cacatttttg cataaatttg acttaatacc gagaaactgg cttttgaaca ccgtttattg
     3901 agcgtctata aactgacctg agtattagag aaacaaaacg gcaaagtagc tccatatact
     3961 ttgccgtttt gttttgaact aataccgaag agaagctgat caaacaacat ttcttttata
     4021 atatagattt tagacatata tatgatataa aaatagtttt cgcaaaaaaa
//
LOCUS       DADVXU010000155         3965 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.162, whole genome shotgun sequence.
ACCESSION   DADVXU010000155 DADVXU010000000
VERSION     DADVXU010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3965
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.162"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(613..1170)
                     /locus_tag="KB953_002742"
     CDS             complement(613..1170)
                     /locus_tag="KB953_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867650.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1349..1960)
                     /locus_tag="KB953_002743"
     CDS             complement(1349..1960)
                     /locus_tag="KB953_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867651.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2073..2330)
                     /locus_tag="KB953_002744"
     CDS             complement(2073..2330)
                     /locus_tag="KB953_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867652.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2927..3205)
                     /locus_tag="KB953_002745"
     CDS             complement(2927..3205)
                     /locus_tag="KB953_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867653.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3239..3490)
                     /locus_tag="KB953_002746"
     CDS             complement(3239..3490)
                     /locus_tag="KB953_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867654.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1201 a          811 c          526 g         1427 t
ORIGIN      
        1 ttcttatttc ttggagttaa acgcttctgt cccagcctcc ttattccgaa taaatggtgt
       61 ccaaaagctt gcatctgcca gattatctca tttcttatga caagctaagc aaattatctc
      121 caccaaataa tttattcata tttttgactt gcgcatacta actaattctt aaattttcat
      181 acaaaatttc tcaatttccc ttaacgagta atatttatgt acggtgattc tcactagtat
      241 cccaatcact ttttaagtta tcccgatttc aattctactt caaaaatagt tgcttttatg
      301 tatgctgtta gttgattaga tggtcatttg ttgagctagg tcgtttattt taaacaagtt
      361 gtttctttag cttataccta tctcgtctac caaacgcaag atggcaaaca caaatgcata
      421 cgcatctgta tttgccactt tgattcgtat aaagctaaca actattaact cctctcagct
      481 aatagttgct ctctttttta ttcagattca tttttgctga tcacagccta gtatttcaat
      541 ggttttgaac actaaaccaa attgttccat tacaacattc actggctgat gaaatggatt
      601 agtagatttc gattattcaa agcgattttc tattggtgga tcgtgttccc aactaataag
      661 acggattcca aacctatcgt acagatcttc taaaccactt ataaattttg cacctatttc
      721 aggtgtttta ttgcgtggat attctattgt tacgccatta aatggatacc aattaggatt
      781 aggctctctt tttttctgta catctcccat agaatagtta gcactttctg ggacataata
      841 tattcttcga ccatcctcta attcatctaa taagatattt cgtgatcgtc tatcacttgc
      901 taaatgtaga cgccacgtgc gataccatgc catgctacct gcatctgcag aaaatccatc
      961 aaataatgca acaattgtat cttctatcac aatcctttcc ccatctactt catattctaa
     1021 tctaaaatca aactcccctc gggtaatttc tggtcttggt ggcttaggac ccaatattat
     1081 tctacttaaa accgcattcg ttcccagatg aaacacagct ataacaacaa taatgattaa
     1141 tattttgttt ctatttgata aatttctcat tattcgcaca tcacctctac attctcacat
     1201 attagttcgt aatactcctt taatatcaaa atatctactg gtcgtaataa tattcgctat
     1261 gaattcctac tactcgtata agaaaggcca aatcatcagc ttttacttgc tgatgatttg
     1321 gcttgtttct atttatcgta attgttttct aatcaccaaa attgttaaca atcggttcag
     1381 atatttccca gttgattaag gtaattccgt attgtgtcaa aacttcttgc gcttctccga
     1441 aattaagtac agttcttatg tatccacttt ctcctaatat aaagtctacc tctcttttaa
     1501 aaccttcctc gagatttcta agggctatat gtggttttaa tgacattgat tgtacatttt
     1561 cccccatata atattgcgca cttcctcgac taaaatagat tcctagatcg tccccaaaag
     1621 gtagatcttc tctaccacta gctaatctac ttctccatct tcgttctctt ttcatagaac
     1681 ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt atcttcaatt atgttaatct
     1741 gtccatctag ttcgtattca atcctaaatg gaaattctcc ataggtgatt tccggttcgg
     1801 gtattttagg tgagattaat gcaaagaaag gaaatattaa gcttggcaac atcaaaaaaa
     1861 ctcctcctat gatcacaatt cccgatgtaa tccaaaatat aatttgtaca attctcattg
     1921 atccattgta ttccatttta ttccccctat tactattcat atccgcctat ctatatcgat
     1981 cttccaatgc agagataatt gcttccatac cttgattttt tactgagtta cctatgcgag
     2041 atatcattct ccactaaggt gcatcatatt gtctatctaa catgattaat tctccaatag
     2101 gtgtatactc ctccaaaaac aacatttaat gcatctccct cgacaacata agctaccatg
     2161 tttgctgtat atgtgtttga gtctaattca taagcagata aatttactgt cgctggatta
     2221 aatataatcg cattcgtatt tatagctact gaatttgctg cttcagcacc accttttgaa
     2281 tgtccgatca ttgtgatttc aatagtagga tttacgtgga cagccctcac cgtttaccct
     2341 gaccattata gaaatcgctt acttttttga actacgcaac aaaggataca atgctcctaa
     2401 cttcactgtc tccatttttg tccactaagg aatataaaaa ataggcatta attcatttaa
     2461 ttcggtcaca acacgttacc ttttaaatca ttatactgta tctcatacat tattatcaat
     2521 aaactattat aaaaatgcaa aaaatgcttt atcaatctat tttgtaattg gtcataatat
     2581 cattgaaatc ataccctgta tcgatttttc ctatgaatct cgtctatttg tgattgacga
     2641 aaaagaggca agacagacac tggaatggta attttaccca ctattttgct ccaactattt
     2701 gtaatcgtac ccacttttga aataccgaga tgatcatttt aattgatatg ctgattccca
     2761 agcaaaaccc caagaaatta aatgctctat cgcatctagc ttcttgggtt ttgcttatat
     2821 aaattccaac atttaaattt tttatgctgt tcggtctcag aaatgccgtt tttgcttata
     2881 tcacaatatc aagatgtcaa attattcgtg ttgccgatga ctttgattaa tttttttcaa
     2941 attgtttatt aagactaatg ctgtcttttt gcagatacca atgttcgaac tttttaacat
     3001 tatcatatga tcctgcaagt tttaacttct taccattaga tgttatcaaa gttataatta
     3061 accttgcctt tttcccatta ggatctttta ttttatgaat tttctgcagg taatctctcc
     3121 taatggcagc tatttttacc tcatcagtgc tgataaacca ttgatcatcc acatacatac
     3181 tgcctgttat gttgtgtttt tccatttctt ccgagaacga aaagtttaag gctcgttctt
     3241 acttccaaag ttgaatgagt ccatggattg gacttagtgt gagaattgag aacaacaata
     3301 agaatccata tcctcccgca aacatcccta aggctatact gtctctccat ccataagtag
     3361 gattagaaga gaaaagagtc accgtttgta ataatatgaa aaggaataat ccagcacaaa
     3421 caccaaagat aacaactcca aacattgaat ttttatacaa tgaccattta tttataggct
     3481 tttctttcat tttacttccc cctactgttc tgttcacacg gaatgaattc aaagttcata
     3541 agccctcctc tttgaacata tgaatacgac ttgctaacta tactttaaaa aagcttgttt
     3601 taagcggtta taggcgagtt gtaatctcta ttcatctcat tagcaaataa cttgccgaaa
     3661 agtctactga aattaaagca gatagtggaa gaattattta gccaggatag acactatttg
     3721 tgaaacacat gtctttactt ttgaaattct atgatgaaaa gccttggaat acagtagctc
     3781 ttaattggta atactttttc cattataaat aacctctttt cattttatat ttatcccctc
     3841 taaaaataat atctaatcta atattttaaa cttatatttc cctcgctgtg ctaatagagc
     3901 attactaaat aaatagaaac tagaaaaaag cattacgcct gccagaataa accaattatt
     3961 tgtat
//
LOCUS       DADVXU010000156         3872 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.163, whole genome shotgun sequence.
ACCESSION   DADVXU010000156 DADVXU010000000
VERSION     DADVXU010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.163"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(716..3865)
                     /locus_tag="KB953_002747"
     CDS             complement(716..3865)
                     /locus_tag="KB953_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HBB6867655.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSVQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         1076 a          800 c          635 g         1361 t
ORIGIN      
        1 actaatacag gtagtcctct tttttgttat tttgatattc taaatcattt tttattggat
       61 tattcccttg ttcgatatag acccaaattg atctatcaat aattatcaaa ctgagtcata
      121 tagagacaaa aatttcaaaa atgacctgtt aaatggtcat cgaatatcat ttaaaatcaa
      181 tacgacggag tcagacacgg agtcaaaaca agcccgctga gagtaaaaaa tatatacagt
      241 cccataggta aagtgccaca ggactgtata tttttttatt tgagattacc tgaaatgatt
      301 ccatttactt tttatctcat tttttcctac tagttagtat caaatactct cctattttct
      361 caataaacta ttttgggtta catggtatct gaaacagcta aaaccttgtc attacagcaa
      421 aaaatcactt tatctccgtt tcatttcaga tacatttcac ttaagcggat tccaaaaaaa
      481 caaaagaaaa aggaacccct agttagccta tgctttttat tggttcctct caaaacacgg
      541 tcatataaca acggggtggg gtaaatgaat gaaaaaggtg gaggaaacat tattcaatag
      601 ctgtcttaaa tacttcgtta taaaatgtga acaaataaaa gacatctatc cctattttct
      661 acacttccac tttactacca tctttgaatc gtacaatttt cttaactctt ctcgtttacc
      721 tcaattcatt ttcaagtgga atcagctcat catctaacat tttacgccat gctcgcttca
      781 tttcttgtgg gtatttaaat ggcttcgctt ctggatggtt ggctttatat ccttcgtaat
      841 ccttactgtt gataattgct ttcatattag gaattggatc ttcacctggc atatattgca
      901 gattagaagc atgtaattct ttctcagaaa caaaccactc attggaaaag acatgaatca
      961 aatcatcact cgcttgtgta aaatagcgac gtttaatgac aaagatatct tcattactat
     1021 ctaaagtccc cacatcaaac gcatttaaaa tttgtcggta aacttctttg actccttctg
     1081 ttttattcaa tttagccaaa gctttttcaa tttcttctcg tgcatctgaa ctattcgccg
     1141 cataagttcc tagtaattga tcaatataag tagaatcaat atcatacaac ttcgtggtat
     1201 tttcactata aaattcgatc gtggaaaaat cttctccctc tttttctggt tcttcctcta
     1261 ttaatgaacc cttcacggtc tcgtacacgc caatttgttc ttccaaaagt tgaatttgat
     1321 tgttcaaggt tgaagatttc accatgtcat cattgtaatc gtcataagtc actaacgatt
     1381 cataagcacg attcaattct tggaatgctt tgacgtactc gatacggatt tcaagtggcg
     1441 tatgttcatc aatggattcc atggatgaaa cctcttttaa cttaccatat gcttgcttga
     1501 atcttttttt ggattcctga taacttggat aaatcaaacc cgattcttct tttgcttctg
     1561 aatacagttt tgtcgcatcc tcgacattct tctccattgt ggctggcttt cggaaagtaa
     1621 caattagtcc ttttgttttt tctggatacg tacggttcgt acgtgaaaaa gcttggatca
     1681 atcctgcata ctctagatta cgatccacaa atagggtttg aatcgtaggt gcgtcaaaac
     1741 ctgtcaatag acgatccact acgatcacta aatcaatttg tttcccaaat tgtttgaatt
     1801 ccgctctttt tcgtgccaaa cgattattga tgtctccgtt ataacgctca atgtctgcta
     1861 atgaccagga agtatcataa tactgattat attcctcaat aattttttgc atttcttctt
     1921 gttggacact tgaatctcgg ctgttttctt ctaatgaata ggtaatagcg attcgtggaa
     1981 aatcaggatc ctccatcgtt cttccttcac gaatgggctg attaggaaac tcttttgtta
     2041 accagtcagg ttctttcgtc atttctttga tcgctcgata atactttttg gccatgtcaa
     2101 tggaactcgt ggttaagatc gcagacttcg ttggacggcc atttcgaaaa tcaaattttg
     2161 tataggcatt gtctggacgg aacattttat gaatgacttt ttggatatgt tcatctgctt
     2221 caaaaacagt agggtccaaa taactttctt ttttgacagg ctccatttga tcaatcatac
     2281 gattgatctg ttccggtgaa tactctgcat atgtttctac ttcttttaat ttccgataga
     2341 ttttattctc taatgaagtc ggctccacgg tgttttcatg ctctacttga aatcctaaaa
     2401 ccgagccatc ttctaatgca ttcttgatgg tataggtatg caagacctct ccatactgat
     2461 catgggtcgt acgtgccaat tgccctttgg cttgtttccg attctcaggg aaaatcggtg
     2521 tacctgtaaa gccaaaccaa gtggatttcg gaaacatttt tttgatttct tccatatttt
     2581 cagcacttaa cgctcgatga cactcatcta ctacaaacac gatatgttgc cccattaatt
     2641 tttggaagcg attggttcct tttttctctt cttgtttttt tgcatatttc aacgccgcat
     2701 ctagcttttg acgggttgta ataatcacaa catttgcatt ggcatctgcc aacagagttt
     2761 cacttaactc tttggcactt cctgttccca cgatcaagct attcgctttg gcgttcccgg
     2821 aagaaatccc agtattaaat tccgaagcaa atttcgtaaa ctctgtggtg gtttgattat
     2881 ctaaatcttt gcggtccact aacatgatcg tccgatcgat ccccgatttt cgtgctaaaa
     2941 gcttagtaga tacaaaacta gtcaacgttt ttcctgaacc agtcgcatgc cacacatatc
     3001 ctgattgatg tttatttgca gcaataaaca aggcttgaat cgcatgtact tgataaggat
     3061 gtaacaccat gaggatttta ttgtcttgat cttcactgac aatcgtataa tccgcaatca
     3121 agcgatgagc atccgggata tttagcactt gtttgcaaaa ctcatataag ttttctacct
     3181 ttttattatc tttcgttcgc caactaaata aaaacttagg atgcaaatcc tttggtaagg
     3241 catttgcaaa ataccgagtg gtttgttcat tggaaaccac aaacaactgt aaggtcgaaa
     3301 agatattgtt ccgaaacata ccttcttctg catatttttt gatttggtta aacgcctgat
     3361 aaaaactatc ttttgcggtt acttgtttca gctcgatttg gacaatcggt aatccattga
     3421 tcagtaaggt gacgtcaaaa cgtcgatccc gtgcttctat gttacttcca cgttttgcaa
     3481 tttgatggac cacttcatag gtagagattc ctccaccaat gtcttgatta gaataaagaa
     3541 tgagcgagac agaacccagt tgcggatctt cccgttcaat cgtgatccga gccattccat
     3601 tttcaccttt taaccatttc gcagcatcaa agggggtttt cgttcgtagt aacaattctg
     3661 ttttgatcgt ttcaaattct ttatcggtca aaggaaaatc attcagttct gcttgattat
     3721 ttgagatgat tttttggcgt aaattgaccc aaagatcttc ttctgatttt agatcgggac
     3781 gatacgtcca ctgattatgc ccttccccca atacacggat caactgttct tcgacttccg
     3841 cttcctcacg gtgactaatt ttcatttatc tt
//
LOCUS       DADVXU010000157         3791 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.164, whole genome shotgun sequence.
ACCESSION   DADVXU010000157 DADVXU010000000
VERSION     DADVXU010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.164"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(95..592)
                     /locus_tag="KB953_002748"
     CDS             complement(95..592)
                     /locus_tag="KB953_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HBB6867656.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(722..1432)
                     /gene="budA"
                     /locus_tag="KB953_002749"
     CDS             complement(722..1432)
                     /gene="budA"
                     /locus_tag="KB953_002749"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HBB6867657.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1445..3109)
                     /gene="alsS"
                     /locus_tag="KB953_002750"
     CDS             complement(1445..3109)
                     /gene="alsS"
                     /locus_tag="KB953_002750"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HBB6867658.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3314..3781)
                     /locus_tag="KB953_002751"
     CDS             complement(3314..3781)
                     /locus_tag="KB953_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867659.1"
                     /translation="MIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLFAWIVSVI
                     MIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVLRIAFYYT
                     LKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTMDL"
BASE COUNT         1127 a          819 c          602 g         1243 t
ORIGIN      
        1 aaaaaaataa aaaaaccgaa aaacagaaat gcccactgtc tttccggctc tttttattct
       61 tgatttctct tcttttttgt ttcaatagac gattttagtt attaataaat gaaaaagcat
      121 ttatattaaa atttgctaat aaaagatcta catatttttc tagaaatgct tgatcgattt
      181 catcataccc tttcgttact ccgcaatcga tatctaaaac accgattaaa ttaccttctt
      241 ttaccattgg cactacgatt tctgattgtg cggctgaatc gcaggaaatg taatttgcat
      301 gggttttgac atcatcaacg atcaatgttt cttgtttttc agctgattct ccacaaactc
      361 cttttcccat cttgatacgt gtgcaagaaa ctcgcccttg gaacggtcct aaaatcaatt
      421 cattaccatc gaataaataa tatcctgcaa agacagtctc aggcaaagtc tcattcaaca
      481 atgcagatga atttgctaaa tttgctaacc agttatcttc tgcttctaat aatccttgtt
      541 gctgtaataa taaaatttcg tatgcatctt ttttctgttt ttctgtccac atgataaaaa
      601 ccgctccttt ttgatctatg gagaagatta tagctcagtt tttttgtaaa cgctaggttt
      661 cctcctcaaa aaaacgagat acgtcaggtt aatcaccttg aacgcatctc gctgtctttt
      721 tctattctgc ttgttggatt tctgctgcta aatcagcata atcaatcgtt tcgtgaacaa
      781 aagtcggatc atcaactgga aaatgctgta caagggacga gatgcggcta atctcaactt
      841 ttccttcagc cacttcaaaa tccattacat gtccgccaaa tgtatgtgta tcatcgataa
      901 aatgcaagtg aaatcccgct gcagcaactc cttggaataa ttcaggcgta aagaacccga
      961 ctaatgttcc ttggacattg ctttgttgga attctggttg agactctgaa atttttgcta
     1021 gtcttctgta aggtttttct tgcttcggca tgatacggat gtgcatattt ttgaataatc
     1081 cagttatctt cactgccaaa aataggtttt ccccggcttc tcttactaat atgtcttttt
     1141 tcaattgttc tccatctgca gtttttgaga cagaaaaact agtatctggc gtaaaatctg
     1201 taattgctgc atagggggta agttcacttc catctaattg ggcaactgag ccatctgatc
     1261 ttccttgata agctacgcca tctaaaaaga tgacttcacc atcaagccca tgcaatgttc
     1321 ccaatcccag agttcctttt tctaatatat gagcaatcgt ttctgttcca tccatcaagc
     1381 cagccatcaa agctcctaat gtcccgtgct ggtataagat tttttcagtc atgtcgacac
     1441 ttccttaata gaattgatct ggtaagattg tttctcccaa tttctcatta tcacggtaat
     1501 caatcggaat atcaataatt acgggtcctt ctgtcgcaaa tccttcttct aaagcttttt
     1561 ctaattcctc tacacttgta gctcggattc ctttagcgcc aaaagcttct gcatatttca
     1621 caaaatcaac tggaccaaag tctacacctg acgcccgctg atatttcatt ttttcttgga
     1681 actctaccat attgtagtgt ccatcattcc aaatcaaatg aacgatattc aattttttac
     1741 gtaccgccgt ttctaaatcc tgggcagaga ataaaaatcc cccatcgccg gaaactgata
     1801 cgatttgtgt atttggacgc accaatgcag cagaaatcgc ccatggcaat gcaacaccaa
     1861 gcgtttgcat accattactg aataacaaat gacgaggttc ataacttcta aagtggcgtg
     1921 ccatccaaat ataatgacta cctacatcta cggtaacagt catatcatct gttacttttg
     1981 actgaagcgt gttaataact tctaaaggat gcaagatacc tgcttctcct tttgattgaa
     2041 caatgtcccg ctccgttaat tctgcttgaa gagatgctaa atattctttt gcatcttctg
     2101 acacttgttg aggctctaat gaacctgtca ataaatcaag agtggctgag atatcaccga
     2161 tcaattctct ttcaggctgc ataaaaggat cgatttcagc tggcgcttca tcgatcacaa
     2221 tgatccgcgc atctttttcc gcattccagt ttcttgcttc atattcgatt ggatcatagc
     2281 caatagcaat aactagatca cttcgtttca aaagcatatc tcctggttgg tttctaaata
     2341 aacccacacg accaaagaaa tgcgcttcta attcacgtga gatcactcca gctgcttgga
     2401 atgtctctac aacaggcaat gctgtctttc caactaattg cctgatggct aacgtttctt
     2461 tttcacttga tcctcgcata cctactaata gtaccggtag ttttgcttct ctaatgcgtt
     2521 cagctaggta acggatatca tctgcagaag cagaacctaa tttaggatcg ctcaatggct
     2581 tgatgacatt cccttggaca ggtgcatcca ctacatcttg cgggatactg ataaagctcg
     2641 cgcctttttt tgaactctta gccatacgat aagcattggc aatcacttct gaaattgttt
     2701 ctggatcttg gatttctgca ctgtattttg tgattggctg gaacagtgct gcattatcca
     2761 tgctttgatg ggtcaacttc aatagatcgc ttcgttttac ttgtccggca attgctaaaa
     2821 ctgggtcacc ttctgctgtc gctgttacca aacctgtagc taagttacta gcaccaggac
     2881 cacttgttgc gataactact cctggctctc ctgtaatacg tccgattgct tgtgccataa
     2941 atgcagcatt ttgttcgtga cgagtcacga ttaattcagg accttgatcc tctaattcat
     3001 taaatacacc atcgattttt gcacctggta taccgaatac ataatcaaca tgatgattaa
     3061 tcaaactttc tactacagat ttcgaacctt gtttcatttt ttcgtccatt gaacatcgtc
     3121 ctttttatgc tttattttta attattagtt agaaataatc acaaataatt gatacagttt
     3181 ctaaacctgt ttcattcaaa agtatagcac gttttatttc atttgtttag ccatatgttt
     3241 caaaaaatga aaaagaatga cagtttttac ataaaaactg tcattcttaa ttgaaagttt
     3301 caatttataa agtctacaga tccatagttc tcatcaattt acgattgttg aagtaaatca
     3361 aaccaaatac gatagaagga cctagtagaa tacaccattg gggctttacg aaaaagaaga
     3421 gaaacaaact tgtcaatagt aaaaaatcta gtaaactcac ataccatttt ttcaacgtat
     3481 agtagaacgc tatccgtaga acttgatttg gtttcgacgc tggatttctc acctgaaaat
     3541 accagtagtt gatgctaaag gaaactgata aacaagcaag taaaacaaac aacggaatca
     3601 accattttcc ataagaaaaa cgtataaaaa agaaacaatc aaaaatcatt atcacactaa
     3661 ctatccaagc aaatagccaa taaaaaaatc cctttagcca aaattggcga aacgtcttta
     3721 tgaacaaacg tactggtgaa aaactttttt cttttgctaa ttcttctgtc atacctatca
     3781 tcacgatgca a
//
LOCUS       DADVXU010000158         3768 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.165, whole genome shotgun sequence.
ACCESSION   DADVXU010000158 DADVXU010000000
VERSION     DADVXU010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.165"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(83..334)
                     /locus_tag="KB953_002752"
     CDS             complement(83..334)
                     /locus_tag="KB953_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6867660.1"
                     /translation="MEQLALVNLSDLKASLAESEIANEVWDTKQAAEYLKTTTRTLTK
                     DAESGKIPAAKVGREWRFSSIALYQYLKGGKTWVNSTEH"
     gene            complement(426..683)
                     /locus_tag="KB953_002753"
     CDS             complement(426..683)
                     /locus_tag="KB953_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867661.1"
                     /translation="MNEEMSQTIKVQKMIDDLTHAIDSQADKIIKELQGQKVWDAKML
                     FKTIKFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(695..1456)
                     /locus_tag="KB953_002754"
     CDS             complement(695..1456)
                     /locus_tag="KB953_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HBB6867662.1"
                     /translation="MNTQKFENNLFELEVKTENGESLFNAETVARSLGFVETKNGKEY
                     VRWRTVNGYLKKYLSQEVAKNDFISEPMVYKLAFKANNVLAEKFQDWLATEVLPQIRK
                     HGMYATDELLNNPDLLIEVATKLKEERTLRLIAEQRVNELQPKADYYDRILNNKGLVT
                     VSTIAKNYGMSAVSFNKLLHELGIQFNQSGTWLLYSKFQDKGYTHIEPFDYEDKNGNR
                     QVKTRMKWTQKGHIFLYETLKKNNYLPMIEREQTA"
     gene            complement(1480..1704)
                     /locus_tag="KB953_002755"
     CDS             complement(1480..1704)
                     /locus_tag="KB953_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF739 family protein"
                     /protein_id="HBB6867663.1"
                     /translation="MSFDYSKLSGRIVEKYGTQYNFSIALGLSERSLSLKLNNRVPWK
                     STELQKAIELLDIPKEEIGEYFFNLEVQNV"
     gene            1864..2610
                     /locus_tag="KB953_002756"
     CDS             1864..2610
                     /locus_tag="KB953_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004564412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6867664.1"
                     /translation="MKQTTQQRLNQLMSERNLKQVDILNMSLPLQKETGIKMSKSHLS
                     QYVNGKSSPDQHKLYLLAKTLNVSEAWLLGYDVPKEDKKNNIPSIETIYNQLEKPRQT
                     KVYNFAEQQLEEQNRGNVVHFPKKEKLPTIKNSASAANPTELVYGDTVVEEEEFERVP
                     NNADFAVPIIGDSMEPVIRNGQFVFVKEQPDVEDGEIAIVEIDGDGVTCKEVYKDYES
                     QTIILRSINELYEDRVVSPEQIRIIGKVVF"
     gene            2686..2892
                     /locus_tag="KB953_002757"
     CDS             2686..2892
                     /locus_tag="KB953_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HBB6867665.1"
                     /translation="MPSYVVLQVVLKEKFIGKGSQNLSELENTINRQCSKGYRLHTIS
                     TTNGGSKGFGGGDRIQATLVFESL"
     gene            2995..3648
                     /locus_tag="KB953_002758"
     CDS             2995..3648
                     /locus_tag="KB953_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867666.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDATSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQVTAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFFKYGIQ"
BASE COUNT         1202 a          711 c          659 g         1196 t
ORIGIN      
        1 accatgtaaa cgatcgtgcc gattaatgct tgtctgcttc cccaaagtcc taaagcatag
       61 acgattaggg gtgcgctgaa cactagtgct ctgttgaatt tacccatgtt tttcctcctt
      121 ttagatactg atacaaagcg atactactaa acctccactc tctgccaact ttcgctgctg
      181 gaatctttcc agattcggca tctttagtca gcgtgcgtgt tgtggttttt aaatattccg
      241 cagcttgctt tgtatcccac acttcatttg caatctcaga ttctgccaaa gaagctttaa
      301 ggtctgagag gttaactaaa gctagttgct ccattggtta tttcctcctg tatttataat
      361 tcgtacattg caataatcga atctataatg atatagattc ggatgaaact gtcatctata
      421 aaacatcagc tgttaaaagt atcggtttca aatagtaatt cttgctcgtt gatagcggaa
      481 gccaactttt cttccaacac atcggcgagc tttctttttg ttggattcat ttcaaatttg
      541 atagtcttga aaagcatttt tgcatcccaa actttttgtc cttgcaattc cttgatgatt
      601 ttatcggctt ggctatcaat tgcatgtgtt agatcatcta tcattttttg taccttgatt
      661 gtttggctca tttcctcgtt catagtgtta cctcctatgc tgtttgttca cgttcaatca
      721 tcggtaagta attattcttc ttcaatgttt catataagaa gatgtgtcct ttttgcgtcc
      781 atttcatcct tgtttttact tgccgattgc cgtttttatc ttcataatca aatggctcaa
      841 tatgcgtgta tcctttgtct tggaacttac tgtatagcag ccatgtgcca ctttgattga
      901 actggatacc taattcatgt agcaatttat taaacgatac agcgctcata ccgtagttct
      961 tagcaatcgt actaactgta actaatcctt tgttgttcaa gattcggtcg taatagtctg
     1021 ccttcggttg taattcattt actcgttgtt cagctatcaa tcgtaacgtg cgttcttctt
     1081 tgagtttggt tgctacttca atcagcaagt ctggattgtt aagtagttca tctgttgcgt
     1141 acatgccgtg ttttcggatt tgaggtagga cttccgttgc taaccagtct tggaattttt
     1201 cagcaagtac gttgtttgct ttaaaggcta atttgtaaac cattggctca ctgataaagt
     1261 cgtttttcgc aacttcttgc gaaaggtatt tttttaaata tccattgaca gttctccacc
     1321 gaacatattc tttaccgttt tttgtttcca cgaaacccaa acttcttgct actgtttcag
     1381 cattaaataa cgattcacca ttttccgtct taacttctaa ttcaaataga ttgttttcaa
     1441 atttttgtgt gttcattttt tcgaattcct ttcttaagtt cagacatttt gaacttctaa
     1501 attaaaaaaa tattcgccaa tttcttcttt tggaatatct agcaattcaa tggctttttg
     1561 aagttcggtg cttttccatg gtactcggtt attcaacttt aatgacaaac ttctttccga
     1621 caaacctaaa gcgattgaaa aattgtattg cgttccatat ttctcaacaa ttcttcccga
     1681 caacttagaa taatcaaaac tcatattagt tcgctccttt ctagttcaaa tgttttgaac
     1741 tttgtaacca aagtataact cctgcataaa acattgtcaa cataaaagtt caaattaatt
     1801 taactttttt attgaactta tgttcaataa ggtatataat aagtatcaga aaggaggtat
     1861 aagatgaagc aaacgactca gcaacgactt aatcagttaa tgtctgaaag aaatttaaag
     1921 caagttgata ttttgaatat gtctttacct ttgcaaaaag aaactggaat aaaaatgtca
     1981 aaaagtcatc tatcgcaata tgtcaatggt aagtcatcgc ccgaccaaca caagctttat
     2041 ttgttggcta aaactttaaa tgtgagtgag gcatggttgc ttggttacga tgttcctaaa
     2101 gaagataaga aaaataacat tccatccatt gaaacaatct acaatcaatt agaaaaacca
     2161 agacaaacaa aagtctacaa ctttgctgaa cagcaactcg aagagcaaaa tcgaggaaac
     2221 gtggtgcact ttcctaaaaa ggaaaaactt ccgacaatca aaaattcagc ttctgccgcc
     2281 aacccgactg aattagttta tggcgataca gttgtagagg aagaagaatt tgaaagagtt
     2341 ccaaacaatg ctgattttgc tgttcctatt ataggcgatt caatggaacc agttatcagg
     2401 aatggacaat ttgtatttgt taaggaacag ccagacgtgg aagacggtga gattgctatc
     2461 gttgaaatag atggtgacgg tgtgacgtgt aaagaagtat ataaggatta tgagagtcaa
     2521 acgattattc tacgctctat aaacgaatta tatgaagata gagtcgtctc accggaacag
     2581 attagaataa tcgggaaagt cgtattctaa aaaatacccc agtcgtagtt ggcgctccgg
     2641 ctaggggtta gtatttatta tcatagtaga aagaaggaaa agaatatgcc aagttacgtt
     2701 gtattgcaag ttgtattaaa agaaaagttt ataggaaaag ggtcgcaaaa cctatcagaa
     2761 cttgaaaaca ctataaatag acagtgttct aaaggttatc gattacacac tatttccact
     2821 acaaacggtg gtagtaaagg tttcggtggt ggcgatagaa tccaagctac tttagttttc
     2881 gaaagtctat aaacaaaaaa acacgcccac cgaccaaagt ttgtggacgt gggatgttaa
     2941 acctatagta ggcttcttta caacttattg tatcaaagaa aagaggagta taaaatgaaa
     3001 aaagttagcg ttatgttgtt gttaagtact gctctgctac tttcagcttg ttcaaataat
     3061 aaaaaagctg aatcaacaga tgccacttct aaccaagaaa caaaaataag taaaacaaaa
     3121 gaaacaactg aaaccagttc atctactagc aaatctacat ctaaaacaga ttctagttca
     3181 acagttacaa gctccaacca agttacggcg gaacctagcc caacagttat aagctccagt
     3241 cagagtacaa tccaaaccgc acctcaagaa gaaacatatg aacagatgaa acaacgcact
     3301 ttacagtcaa ctccagctga tcgtgcaaat tggtccaaca aagagtggga agctttcggc
     3361 gtggcccttt atgaaaatgg attgactaca gatgatgctg gcaatattat cagtcaagat
     3421 cagaaagaac aacaagcagc atctcaacaa aatccagaag accaacaaac aagcgctcag
     3481 caagacgctg acactttatc acttactgat tttgttaaca aatacgggat gtcgcctgtt
     3541 gcatggaaag tacagaatgg aatgtctgaa gaagaagcat tgcgtacaac acagcaaaag
     3601 acttccggtg aagttcaatt aggatttttt aaatacggaa ttcaataata tatttttatg
     3661 ccctactatt ttgcctataa tctctaaaaa agttataaat aaaaaagccc gtgctgcaac
     3721 acggactcat acctcatttc tgagatcaca aatatattat aacaagaa
//
LOCUS       DADVXU010000159         3738 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.166, whole genome shotgun sequence.
ACCESSION   DADVXU010000159 DADVXU010000000
VERSION     DADVXU010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.166"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            72..155
                     /locus_tag="KB953_002759"
     CDS             72..155
                     /locus_tag="KB953_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HBB6867667.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            280..1017
                     /gene="erm(B)"
                     /locus_tag="KB953_002760"
     CDS             280..1017
                     /gene="erm(B)"
                     /locus_tag="KB953_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBB6867668.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1280..2143)
                     /locus_tag="KB953_002761"
     CDS             complement(1280..2143)
                     /locus_tag="KB953_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HBB6867669.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2145..2417)
                     /locus_tag="KB953_002762"
     CDS             complement(2145..2417)
                     /locus_tag="KB953_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBB6867670.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2434..2643)
                     /locus_tag="KB953_002763"
     CDS             complement(2434..2643)
                     /locus_tag="KB953_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HBB6867671.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(2742..3638)
                     /locus_tag="KB953_002764"
     CDS             complement(2742..3638)
                     /locus_tag="KB953_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBB6867672.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
BASE COUNT         1148 a          654 c          579 g         1357 t
ORIGIN      
        1 aaattttgta taataggaat tgaagttaaa ttagatgcta aaaatttgta attaagaagg
       61 agggattcgt catgttggta ttccaaatgc gtaatgtaga taaaacatct accgttttga
      121 aacagactaa aaacagtgat tacgcagata aataaatacg ttagattaat tcctaccagt
      181 gactaatctt atgacttttt aaacagataa ctaaaattac aaacaaatcg tttaacttct
      241 gtatttattt atagatgtaa tcacttcagg agtgattaca tgaacaaaaa tataaaatat
      301 tctcaaaact ttttaacgag tgaaaaagta ctcaaccaaa taataaaaca attgaattta
      361 aaagaaaccg ataccgttta cgaaattgga acaggtaaag ggcatttaac gacgaaactg
      421 gctaaaataa gtaaacaggt aacgtctatt gaattagaca gtcatctatt caacttatcg
      481 tcagaaaaat taaaactgaa cactcgtgtc actttaattc accaagatat tctacagttt
      541 caattcccta acaaacagag gtataaaatt gttgggaata ttccttacca tttaagcaca
      601 caaattatta aaaaagtggt ttttgaaagc catgcgtctg acatctatct gattgttgaa
      661 gaaggattct acaagcgtac cttggatatt caccgaacac tagggttgct cttgcacact
      721 caagtctcga ttcagcaatt gcttaagctg ccagcggaat gctttcatcc taaaccaaaa
      781 gtaaacagtg tcttaataaa acttacccgc cataccacag atgttccaga taaatattgg
      841 aagctatata cgtactttgt ttcaaaatgg gtcaatcgag aatatcgtca actgtttact
      901 aaaaatcagt ttcatcaagc aatgaaacac gccaaagtaa acaatttaag taccgttact
      961 tatgagcaag tattgtctat ttttaatagt tatctattat ttaacgggag gaaataattc
     1021 tatgagtcgc ttttgtaaat ttggaaagtt acacgttact aaagggaatg tagataaatt
     1081 attaggtata gggcacctct aataactacc aattaattca cctcaaaatg aaattagaaa
     1141 aaaacagcga aatttttaaa tctatttctt atcgatacaa attcctcgta ggcgctcggg
     1201 accccaacat gaggaaatgt ttttataaaa aaagagtagt taccgttttt tggtaactac
     1261 tcttttcctt ttttaaggtt taaatacctg gaagtttagg tgttttgggt attggtggtg
     1321 taggtagctg caagctttcc attttttgtt gaattgcttt aaattcaggg gtttcttggt
     1381 gttgattttg aaccattttt tgctctattc tctccaaagt cggttgaatt tctttccctg
     1441 atactttacg atttaattct ctttctaagg tctctttcgg actaatggaa ggcgtttcta
     1501 agcttgaata gagtttcact ccttctctat tgtaaagcct tatatcgcta aataagcccg
     1561 ttttatgaag ggtttctaaa ttggtcggta agtttttgac aacaatatca tgcgcttgtt
     1621 ttggtgttgc cctagctgtc attggatcat ctgcatacat ggtttcatat cgttcaatcg
     1681 ttcctaagta tgattcaatt ttcggtactg ccatgacgta tatttttgtt tcataacctt
     1741 tagattgaag cattgtggct gtttgaatgg gaacgtctgt tgttcgtcct gttccttcaa
     1801 tcactaaatt atagccttga tcgcttaaac ggctaataag tgcttctgtc atgcgattcg
     1861 aataaggggt aacgtgtttt actacgtctt tttcatagag tttcactaat tcgtcaaaat
     1921 ttgggtgctg ttgtttaaag gtatcattat caatgacaat aacattccct tgtgtttctt
     1981 caaaaattgc tgatcgcaaa ctggttttcc ctgaccctgg ttgcccacca agtaaaaaag
     2041 cggttggcga ttcaaccgct agtctatttt tagttagtcg ttcaacattc ttttctaaac
     2101 gatcttcaaa ttgcttttcg gtgaagtcag taatattcgc catgttaagc cactttctct
     2161 ttattcaaaa tttgttttga atattgattc attgaccgcc aatactcatg aatagcttgt
     2221 aattcttcaa atggttgttg aaataaatca acttcttgtg ccacttctaa ttgatttaaa
     2281 agattcactt ctagtaaacg agtattttta gtatctagtt catgattcaa aacataattt
     2341 aatacccgat tataaacact tgccgataac tcattaatga tttcaatttc aaatgttttt
     2401 tcatacgtaa ctgccatttc attttcactc cttttaaagt ttgtcaggta aatatttccg
     2461 tatatattct tctaaggctt gatccataac ttcttttacg tttccgccat tctttgctgt
     2521 ttcgattttt atgatatggt gcaagtcggc acgaacacga accgtcttat cccccattat
     2581 atcttttttt gcgctgattg gcgtatcatt tcgttttgct ttctgtgcgc ctaaatttcc
     2641 cacgatcgct cacttctttc tatttcttct tactatttat tttatcatca cctatcacaa
     2701 atcacaagtg atttgtgatt aatcacttgt gatttgtgat tctattcttt ttcattttct
     2761 aattgaatga ttcgctcaat catttcaaaa aatacgttct catacataga caaaactttt
     2821 ttgtcatagc ctttgtgttc tgtaatgcca tttttagacc aagtacttac tttattactt
     2881 cgcttgataa tatttcggaa aaccaaatta tcctctttat gttctttgta cagttcttcc
     2941 aggtttgatt ttatcgttgc gctgtccgta tcaactaaat aaggaacaaa accaatcata
     3001 tctagtccag ggttaaactg ttcttgcaaa tcaatcaaat aggaaatata gttttgaatg
     3061 ttgtttgtac tttcttcttc tgcttgtaaa gggatcataa cgtaatcact cgccacgatt
     3121 gcattatttg tataaacgct tggcgttggt acagtatcaa taataataag atcatagtca
     3181 ctttttaaag gtgctaaaag agtagcaagc aatctacttt cattttcaaa cgtccatgag
     3241 cgagttaatt ttggcagtaa catcaaatca aacgtgccag ggatcaagtc taaattatca
     3301 gtcaaatgaa taatagaaga agccaagttt ccatttttca agccttcata aaaattgaca
     3361 cgtggcaatt ctacctcaaa agtttttgct aagtcttttg tcaatgttgc ctgtaagtcc
     3421 ttatcgatca ttaaaacttt taaattcaat ttgtctgtca agtaagcaaa catagtcgat
     3481 aatttggact ttccaacacc acctttaaag taattattca agataataat tgcttcattt
     3541 ttattgttta aaatacgtct taattcttca agtattttta gtttttcctt ctccataact
     3601 acgccccatt ctttttttgt atagtaatat tgtatcatta agaatcacaa atcacaagtg
     3661 atttgtgatt aatcacttgt gatttgtgat aagtgatttg tgattaatat ataaaagccc
     3721 tctttaaagg gcttttat
//
LOCUS       DADVXU010000160         3719 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.167, whole genome shotgun sequence.
ACCESSION   DADVXU010000160 DADVXU010000000
VERSION     DADVXU010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.167"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            163..1878
                     /locus_tag="KB953_002765"
     CDS             163..1878
                     /locus_tag="KB953_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6867673.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            1881..3644
                     /locus_tag="KB953_002766"
     CDS             1881..3644
                     /locus_tag="KB953_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBB6867674.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
BASE COUNT         1109 a          728 c          738 g         1144 t
ORIGIN      
        1 ttttttttaa aaaaaatagt ctattcaatt tctaaagctt cttcataata aaactttcat
       61 ttatgaaaag gcttgttctc ttttcattct aaatttccta agctacaatg tggtagtact
      121 tgttttttca attgtggaca aataaataat aggaggaatc atatgattca gttattacgt
      181 tatgcaaaag actatcgtaa gcaaatcatt ttgggcccgt tcttcaaatt tcttgaagct
      241 gtcttcgaac ttgtcctgcc tttaatgatg gcttccttga tcgataatgg actaaaaatg
      301 aatgaccgag gaaaaatcat tgaaatgggc ttatggatgg tcgctatgtc tgtgattggt
      361 ctgatttgtg ctatcatttg ccaatactat gcttcagtag cttcacaagg atttggtaca
      421 gagttacgta atcagctaat caaaaaaatc aatactttct ctcacaacga gttgaatcat
      481 tttggaacag ataccttgat cacccgaatg acaaacgaca tcaatcagct tcagctagct
      541 ttagcaatgg tcattcgttt attgatcaga gcaccgtttt taagtatcgg gtcagtggtc
      601 atggcttttg tgatcgactg ggaagtaggt ctgtttttct tagcattatt gcctattttc
      661 tcaatcattt tattttttat catcaaaaaa accgttcctc tctaccaaaa agtgcaagaa
      721 aaactagatc agctcaatga acacgttagt cagaatctta gcggtgttcg tgtcatccgc
      781 gcatttgcgc agacaaaaaa agaaacagag aagttcgata aaagcacaaa tgatctagcg
      841 aacaattatt tacgtgtttc caatatctct gccctattat cacctgctac aaccttgatc
      901 atgaatgtcg ggatcatctg tttgctgaca gttggcggga tcaaagtaaa tattggtagc
      961 ttgcaacaag gacaagttct tgctttgatc aattatatga atcaaatgct tttggcactg
     1021 atcgttgttt caaacctcgt cgtgatcttc acacgtgccg aagcttcagg aaatcgggtg
     1081 aaagaggtgc tagacacaga aaattcgatt cttgaagctg aggtcagtac tactcccgat
     1141 ttttcttctg aagcaattat cgcttttgac catgttgact tccgttatac tcctgattct
     1201 ggtctttctc tccaaaatat tacatttcag ctgaagcgaa atacagtcct tggaatcatt
     1261 ggtccaaccg gaagcgggaa aacgactttg acacaattga ttccgcgttt ctatgatgtc
     1321 agttccggta cagttataat ggacggaatg gatgttcgtt cttggcagct ggatcatctc
     1381 cgtcagcaaa ttgctcttgt tccccaaact tctgtactat ttaccgggac tattcgtgaa
     1441 aaccttcaat ggggtaagaa agacgccaca gaaaaagaat gttgggaagc tctgaaaatc
     1501 gctcaggcag aagaatttgt ccgtcagctt ccaaatggtt tagatacaaa agtcatggaa
     1561 aatgggaaaa atttctctgg cggacaaaaa cagcgactga cgattgcaag agctttgatt
     1621 gccaagccag cattactgat tttagatgac tctttaagtg cgttggatta tcaaacagac
     1681 ttggatttgc gtaaggcgct acagacttat cttggcagta cagtgattat tatctcccaa
     1741 cgtgtccgct ctattcaaga agcacatcat atcttagtga tggatcaagg taagattgct
     1801 gctcagggta cccatgaaga attacttgct caatcagcag aatatcagga aatcgtcgca
     1861 tcacaggagg aagaatagtt atgaaagtaa aaaaattatc cttgcagacg ctccgccgat
     1921 tcattcctta ccttactgcc tatgcaaaag acatttggct agctgtgata ctaggaatca
     1981 tcaatgggat tgcaacagtt gtgatgacgt tacaaattgg tcaaagtatc gatcaaatga
     2041 ttggagaagg acaggtccgc ttcaatcgac tgttccattt acttattcta ttcggtggta
     2101 tcgttttaat caacgtagtc agtcaatggc tcatccaagt gttaagcaat cggttagctt
     2161 acttatccgt tgcaaaatta agaaaagatg catttgctca tctgaatcga ttgccacttc
     2221 gttattatga tcaaaactcc cacggaaata tcgtcagtcg atttacaaat gatatggaca
     2281 atatttccat tgcaatttct gcggccttca atcaattatt ttctggagtg gcaatcatca
     2341 ttctttcgtt tatttttatg atgcgtctaa gtccacttct gacattggtc gttctttgtt
     2401 ctacaccaat tattttcctt gtgagttgga tcgttgctcg tgcttcacaa acaaatttcg
     2461 atagacagca aaggattatt ggggatatct cgggctttgt gaccgaacga atcggtaacc
     2521 agaaaatcgt gaaagctttt cagcaagaag aagctagtca acagcaattt gaactgctaa
     2581 atgctgatct atatgaaaaa ggacaacggg cacaattttc ttcttccttg acaaacccgt
     2641 cttctcgttt tgtagatcac cttgcttatt tatcgattgg tttagttggc ggcctattgg
     2701 cattgaagag tggttcgtca atcactgttg gtgtgatttc cagctttacg atctactcaa
     2761 gtcagttttc taaaccattt atcgaacttt ctggaatcac gacacaactg caaaccgctt
     2821 tttctggtct agaacgagca tttgatttga tcgatcagcc agaagaaaag cctgatttgc
     2881 cagatgccta tgccttggaa cccaaaaata tcaaaggaaa agtctctttt gatcatgttt
     2941 ctttttctta tgaccccaag cagcgtttga tcgaagattt caattttact gcttatccag
     3001 gtcaaaccat tgctatagta ggaaaaacag gggccggaaa atccacactt gttaatttgc
     3061 tgatgcgttt ttatgacaca acaaaaggaa caattacagt cgatgaccat gacatccgct
     3121 ctatccaacg tgatacatta cgtaaaagtt ttggaatggt gttacaagat acttggctat
     3181 ttgacagtac attgcgagaa aatcttcgtt ttgggaatcc ggaagcaagt gacgcagaaa
     3241 tcaatgatgc cctaaaagcc tcttatatgt atgaatttgt agaacgtttg ccggaaaaat
     3301 tagatactaa gattggcggt caagggttaa agatatcaga tggacaacgc caactgttga
     3361 cgattgctcg aacgatgatc agtaatcccc ctatgttgat cttagatgaa gctaccagct
     3421 cagtcgatac attgacagag aaaaaaatcc aacaagcctt cttagcaatg atggaaggaa
     3481 aaaccagttt tgtgattgcc catcgcctgt ccacaatcaa aaatgcggac cagatattag
     3541 tgatggatca gggagctatt gtcgaaatgg gcacccatga tgagctgctt caaaaagatg
     3601 gttattaccg acggctctat caagcacaat tcacagcaca ataaaaacaa gcagtcttct
     3661 gggatgggaa gactgcttgt tttttattta gcgtggatcg ctaaacttct taaaatttt
//
LOCUS       DADVXU010000161         3691 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.168, whole genome shotgun sequence.
ACCESSION   DADVXU010000161 DADVXU010000000
VERSION     DADVXU010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.168"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            129..743
                     /locus_tag="KB953_002767"
     CDS             129..743
                     /locus_tag="KB953_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBB6867675.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(908..1150)
                     /locus_tag="KB953_002768"
                     /pseudo
     CDS             complement(908..1150)
                     /locus_tag="KB953_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1354..2673)
                     /locus_tag="KB953_002769"
     CDS             complement(1354..2673)
                     /locus_tag="KB953_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HBB6867676.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            complement(2828..3415)
                     /locus_tag="KB953_002770"
                     /pseudo
     CDS             complement(2828..3415)
                     /locus_tag="KB953_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1171 a          648 c          623 g         1249 t
ORIGIN      
        1 cctccatcat tagtttttgt ctatataagg tttaccctaa atagacactg gtataacatt
       61 gaaaaatatg gcttcctcgt gtatgattag ggtgtatttt aaatagttgc agaaagtagg
      121 taaaagtttt gagagttgca tacatacgtg tctcatcgat cgatcaaaat gaacaacgcc
      181 agattgaaga aatgaaaaaa ttcggtgcag agagaatttt catcgagaaa caatctggcg
      241 caacgattac tcatcgacct gttttccaag aagcattaga ttacgtccga gatcaagata
      301 tttttattgt ggaagctatt gatcgacttg gaagaaatta tgacgagatc atcgcttcag
      361 tcaattactt gaagaagaaa aatgtgaagc tgatcattac tagtttaccg atcatggctg
      421 aagcaattgg caatcctctc ctggataaat tcattaagga tctgatcatc caaattttgg
      481 ccatgatcgc tgaacaagaa cggactgaat cgaaacgtcg ccaggcacaa ggaattaaga
      541 tcgcaaaagc aaacggcgtc tacaaagggc ggcccaaatt atacagtgca gacgcaaaag
      601 atccccaacg tcgccttgtt tacaaaagca tcgttgaaga tcttaaaaat ggtgttgcga
      661 ttgctaagat tgctaaagac tacaatgtta cgagacaaac cgtgtataga ataaaaaaag
      721 acagcatggt caatgacaaa tagttgatca tgctgctttt attatcttct catctttaat
      781 ttcctacata taattactat ctataaaatt aagtcttact cagaacagat aaagcataaa
      841 attccaacaa accatttttt acttaactaa ataaagagtc tgttaatata atcttcactt
      901 atttacgtta atcccctact gtatttgttg gttccttatc tatattttct acaaaatcct
      961 tattatcttg tgatttagct tcgtataatt ctttgtatgt cgacacattg gttatactaa
     1021 ttcctatagc tagttcttga gactctactt ttctctctag ctcctttgac aaagtgattt
     1081 gagatttatt tggttccgtt ggagtcaatt ctatagattc ttcataaaaa tctctataca
     1141 taaagctatc cctgaataat tcatagcttt aattctttgg cacattttat tgaaatatgt
     1201 atcactaata tccctatcaa ctgattttga ctaaaaagtt acttcaaaca ttcactgttc
     1261 aaaaatattg aggaataaat tttgggtgta aggcattttg agcatacttc tccctggata
     1321 acttcccagt aaaaaaatcg ttagattgtt ggattagatc cattgactgg ctcgcctcag
     1381 gcgagcaaag accctgtaga attcattctg atacacatga taactagata atttgagata
     1441 gactcgtcta cccgtttgga ctaatttccc agcaactttc agaaacttca atcgaataga
     1501 atgaatggtc attccctttt ggacttcttc aaagccaatc gttcttaaga agttgactaa
     1561 gttgtaagct atcaagctga gcatcattcg gacatgattc tccaaaaagc gaggactgtc
     1621 tgttttgtca aagtaaaagc cagctttcgc ttctttaatg aagttctcca ttgtgccacg
     1681 tttggcatag atagaaaaga tggtatcagg agaaacattt tctgatagat tcgtcacgat
     1741 aaattcatgt cgaaagagta gttcgcccgc ttcacgtgtt gagcgtatac acacgcgacg
     1801 actttgtgac caggattgtg cttgataagt ggtcgagaag tactgaactt ctcgttcttc
     1861 ccacttttga ttatcgccat aaagtactga tttctcagct atttgaccta gtctacgatt
     1921 attcttcagt cgaataacat aatgactctt ttttgattca cacgaatcat acacaccagg
     1981 tgtagcgaac ccgctgtctc cacgaaccaa gatgtcagtg tttggtagag agtgattata
     2041 gtgctctaat aaaggtgtga gaaactcctt tacgcctttt gatgtatatt gatttcctga
     2101 acgtagttca gcttttaaga agtcaccagt caatccgtca aaagctacca atggatggta
     2161 accataggtt tggtagtgtg tattataatt cgtttgttct tggtgaccaa atgtatctga
     2221 atgggtcgaa tctaaatcaa tgattaattc cgtatcatta cggatgaggc gtgctttatc
     2281 gataagttct tggttcaaag cttgaagttc atggatattc tcctcagata gtcgatctaa
     2341 aaatcgggaa agtgaagatt gagaagcgag ttctttccta tctaaaacag ctttaaacac
     2401 aggatcttgt ctcaggagat tagctgcaga atcagctgag taaccagcaa ttaattgcat
     2461 aatgaactgc tcaagtatgg ataagttgtc atgagtaaaa tacgctcgtt cgtcttcaat
     2521 gtgaatgtgt tgcttagcca gttcagaaaa accaaagatg ttcatcagct ctttcactag
     2581 gacgagaccc gaatcactcg ataattgacc acctgtatga gaaatagtga tatttgaatt
     2641 gaattttact tggtttttgt gtaagctagt catgaaaaga actcctttct tttggttggt
     2701 ttagtcactt taaccatagc agaaaggggt tcttttttca tcacttaacg ggtgaagatg
     2761 aaaaagtaat tgtaagctgc cgtgaggcag tttcacatgg ttttaattaa aagtatgaat
     2821 tattcaggct atcattaatt ttataaaaaa aattaaatgt ttttagcact gccatataac
     2881 attcaaaaca ttcttctata gttggtttac ttccttgagg agaatgaacc aatttattac
     2941 gtttttctat gaaattttta atttcctttc ttatttcttt cggaaactca atattaaagt
     3001 accctttaaa catgctccct aataatttgt ctacaggggg agattgtaaa ttctcaaata
     3061 ttagagttaa atcaggtttc tcttgttgat aaaattcctt tacccctatt tcacaggctg
     3121 taattatcga aataaaccta tctctagtat ttgatatatt tttacttctt ttaaggagta
     3181 taagagaaaa ggggatactt tcgtgtccat gcatgtaatt aactaattgt tgagcttcag
     3241 aagttatatc tctaattata tagttaacgg aattaggaag tgtctttaac tctaatgaaa
     3301 tttctatcca attactatct tctaatctgt atatgctcga agtataattt gattcgtcag
     3361 caagcatcgc tacatcccaa acactgatca taaaatcttt taaacgttca atcatttcag
     3421 caatattgct aaaatcagct aatttttctt tagaataaaa aaaaacattt atatcagaat
     3481 caataaattc tattttttta ttactatatc gtcatcatta tcttgaccat ttatgtttat
     3541 tgttccctga gtggggagta cttctttcaa attcaagtta aatttatatt ccattacttc
     3601 tcctccaata tataaaaatt acttaactgt atccaaattt aatatgtaaa taaatcatta
     3661 atttttagct aaattctatg agaagtttat t
//
LOCUS       DADVXU010000162         3677 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.169, whole genome shotgun sequence.
ACCESSION   DADVXU010000162 DADVXU010000000
VERSION     DADVXU010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.169"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            64..972
                     /locus_tag="KB953_002771"
     CDS             64..972
                     /locus_tag="KB953_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HBB6867677.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..1592
                     /locus_tag="KB953_002772"
     CDS             972..1592
                     /locus_tag="KB953_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867678.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            1596..2045
                     /locus_tag="KB953_002773"
     CDS             1596..2045
                     /locus_tag="KB953_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867679.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            2045..2539
                     /locus_tag="KB953_002774"
     CDS             2045..2539
                     /locus_tag="KB953_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867680.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            2553..2984
                     /locus_tag="KB953_002775"
     CDS             2553..2984
                     /locus_tag="KB953_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867681.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            2986..3123
                     /locus_tag="KB953_002776"
     CDS             2986..3123
                     /locus_tag="KB953_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBB6867682.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            3160..3453
                     /locus_tag="KB953_002777"
     CDS             3160..3453
                     /locus_tag="KB953_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867683.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            3450..3674
                     /locus_tag="KB953_002778"
     CDS             3450..3674
                     /locus_tag="KB953_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HBB6867684.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
BASE COUNT         1324 a          546 c          814 g          993 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taagaataca gaggttatgg aagcgactat agttgaaaat
      181 ggtcttccct acgatttatc agaatgtaag gtatattttg aatcagttgt aggtgggaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat taactatatt
      301 tttgataaat attccatgca gtgtttacat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcattaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaatcg aagatttaat tgcggatatg
      481 acggacttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgaac gttggagaca agaacaggaa cacagtttcc aagattggag agaaggacaa
      601 gaatcagact atttggcatg gtttgaatca atcaaggata ttttaaagac gattgatcca
      661 ggtggagtca tgttagccga actaacggat gcgcgagtag atattcaagg agttcgtcat
      721 gcgtctattt cagagcgttt gctggcagat atggactatt tatatcagaa attgcgagca
      781 acgcttttca cgattgaata cggggagatt gaagtgactg atattttgca ggatgatctc
      841 ttttcagata atcatgaagt cgaaaaagtt ggaactgtag cattcccgat tgaagaagga
      901 gccttgatca tcgcaaccgt tgatgatcca aagcaaaatg tgttcactct tgagaaagtt
      961 ggggtgatct aatggctaaa actaaacgaa tgatggaaac ggatgaaaaa acgggtgtac
     1021 aacgccaatt ctttccaatc acacatgctt ccgcagttct tgggttagaa gaaataatag
     1081 caggagaagc aacagtttta tctgttaatg gaaaaattgg agccgtcgtc attactaaag
     1141 aagatttggg attagagaat gttctcacag aattacccta tgcaagtgaa gaagatgacg
     1201 gcattatcac ggcagaaatg tatcaaaaaa ttttaaacag tggagaaggt gactacgtgt
     1261 tgccagttgc cactatcgac cgtttgggtg gcataaaagt tggtgaatta ttgacgattg
     1321 atgagacagg gaaagtctct gcagtcagac aatctgatgt caatttttcg ctggagttaa
     1381 aagaaaaact cgattcactg aaaaattata ctgctggaga aaacatcact attgatgaag
     1441 atggaacaat tagttctaca ggtggtagtg ggactggtgg tgtcaatcag tcatatgtag
     1501 atcaaaaatt ccaagaagct gtaaatcaag cagaaaatta cacaaacgaa agaattccaa
     1561 attttacttt tgaaaaaatt ggggaggtat agagaatgac agatatcgta aaaataaaac
     1621 aaagcaatgt acaggtttat ccacaaactc attggaatgc tatagaaggc aaaccaacaa
     1681 cggtgaaggg agataaaggc gatcctggcc aagcagcaac aattaccata ggaaccgttt
     1741 ctagtggttc aaccgcttct gttacgaatg taggaacatc atctgcagct agatttaatt
     1801 ttgttttacc aaagggagat aaaggtgatc ctggaataaa tgccacaact acagctgtag
     1861 ctacaactac agcaaatggg ttgatgtcgt ccacagataa aactaagttg gatggaattg
     1921 cagctggagc acagaaaaat ccaggtaatg ctacaactac gacagcaggt ttaatgtcag
     1981 caaccgataa agtgaaactt gatggattag cgaatattac atttgagaaa gtggggacgg
     2041 tttaatggct gatattgttc aattaaaaga agacggagtt gctaaatact taaaaacgca
     2101 tgtagaagct attgatggta aagaagcatt ggtgcagaca gatggagacc aagccattgc
     2161 aggacataag aatttttcag gttctgtaac aatcaataat aaacgtgttt tgacgactga
     2221 tgataacaga tatgaagtgg taaatctggt tgtcactaac ggtaatacag ggacagcaaa
     2281 gctttatcgt gaaggaaaaa cagtttctat atattttgtg gctttaaacg gaaaaagtag
     2341 tggcgggaat gattcggtta ttttaactgt tccagaaggc tatcggacac caattagttt
     2401 tgaacaactg gttgggtcaa tagaccgttc tactttgaac agtgctcaac tatctattgg
     2461 cgcagacgga gccattaaat ggagaagaaa ttcaagttat ggatcagctt attcatttgt
     2521 tatcacttat tcaatttaag ggaggaaatc taatgaaagt agtttacaaa tcaatcaagc
     2581 cttacggatt cgagcaaatc attttgaaca atcaagaaaa tatccctgaa aactgtacag
     2641 agattaaacc accagttcct aactggagac caagatttga ttttgataaa aaacagtggg
     2701 ttgagttagc tactgaagaa gagaaaaaag ggaatgctgt tgatagcgta gatgaattgg
     2761 ctaatattaa ggcattatac gaaacgctga aggcagaaaa tgatgaattg aaacagctaa
     2821 attctaaagc aatgctaaac aatgtagcaa tcaagcaaga aaatgtctta ttgaaagaaa
     2881 agtcagaaag tttagcgcag ttgaattcaa aaacgatgct tgcttctgta caaaatacca
     2941 aggaaatcga agaaatcaaa aaacaattac aaggtgggaa gtaacatgta ttcatatgaa
     3001 gatatcaaac taatgtatga ctggggctgt ttcactaacg aacaagtaat ggttttcgtt
     3061 ccattatgca ttacagacga agaagcagat aaaatcatta ataaagataa gagcgcatct
     3121 taattgatgc gtttttattt aaggtaaagg agttgtcaca tgattaattt aggggaatgg
     3181 ggaatgatag caggatcaat aaccgctatc gtttctttga ttttattagt aattagaccg
     3241 attgctgcat ctttctcgaa aattactgag actctttcaa aagtaagcca caatttagat
     3301 ttgctgacta aagatttaga atcgagcaaa tcagatcgat tgatgattca tgaagaacta
     3361 aagaaacacg atgaaagatt agatacacat acagaaaaat tggtggaaca cacgcaacaa
     3421 atcaaaactt tgtttaggga gagaagaaaa tgaataataa aacgttcgaa gtactaaaat
     3481 ggttcgcact ggtaattatt cccgcactag ctactttcgt ggggttagtt ggtaaagcgc
     3541 tcaattggca gtacacagat atctgtgttg tcatcattac tggttttggc gcgtttttag
     3601 ggagtgtgtt gggtgtatca aatcgaacct acaaaatgtt ctcggctgaa agcgaagaag
     3661 gaggaaacaa atgaaaa
//
LOCUS       DADVXU010000163         3637 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.170, whole genome shotgun sequence.
ACCESSION   DADVXU010000163 DADVXU010000000
VERSION     DADVXU010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.170"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(256..933)
                     /gene="nth"
                     /locus_tag="KB953_002779"
     CDS             complement(256..933)
                     /gene="nth"
                     /locus_tag="KB953_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBB6867685.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(940..1638)
                     /locus_tag="KB953_002780"
     CDS             complement(940..1638)
                     /locus_tag="KB953_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBB6867686.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1906..3090
                     /locus_tag="KB953_002781"
     CDS             1906..3090
                     /locus_tag="KB953_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HBB6867687.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3096..3539)
                     /locus_tag="KB953_002782"
     CDS             complement(3096..3539)
                     /locus_tag="KB953_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBB6867688.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT          939 a          727 c          609 g         1362 t
ORIGIN      
        1 cgtcacttat ttatataatc ctttggaata ctggacttat tttataggta tattggaagg
       61 cattattttc tacatcgggt attttcccaa taaaaataac aacttttatt tttatcgcaa
      121 taaattgtaa taagttacca tcctagctac tgagtatgta catttacttt ttcatgcaaa
      181 aaagataaac gaaacagctt ttctccataa taacaagata attcatacat ttatttgcaa
      241 ttaaagaaac cacgtctaca ggcgtggttt cttttttagc gccttttctt ttagacgcat
      301 ccgatttttc ccatcttgac atattgatag caaaggacat acctcgcact tgggatttct
      361 tgccgtgcaa tgatatctac caaaaaaaat cagtgtatga tgtgtcttta cccataattc
      421 ttgtggtact ttccgcatca gtgtttcttc tacttccatg accgtagcat ctagtttgca
      481 gatcctcagg cgttttgaga cacgttccac gtgggtatct actgcgattg ctggtatccc
      541 gaatgcatcc cccaatacga catttgctgt ctttctgcct acaccaggaa gagacatcaa
      601 ttcttctctt gacgttggca cttgcccatc gaaacgttca atcagctgct gtgcacacgc
      661 tttgatgttt tttgctttat ttcgatacaa accaatcgtt tttattttaa gaatgatttc
      721 atcgattgaa gcttctgcta acgcatcggg tgtcggaaaa gaggcaaaca aatctggcgt
      781 tgctttattc actgatacat ctgtcgcctg ggcacttaag atcactgcaa tcaataactc
      841 aaatggatta ttgtgcttga gttctccatg tgcttcggga aacattccat acatcgtttc
      901 tagagcttcc attgtttttt gtttgcttaa catattccgc tactcctcca accaattttt
      961 cagtgagact ttcggcaatt cttcttgttg agctgactgt tctacttctc tttgaagaag
     1021 ctgctgtttt cttcgttttt ggtcttcttc cacttgttgt tttgtacgta gattttttct
     1081 ttcccacgat agcaaaatgc ggtcaacgta attaaaacta taagcctgat ttaatacggc
     1141 ttcccgcagt gctagcttca aaatttccgg ctgataatgg tcttcttcta gccattgtcc
     1201 gatccgctgg aactcgatcg aagaaagcgg gcgtccaaat tcttgttcaa ataattgata
     1261 cactgactgg atttcttttt cttgtgtttt ttcttcgtat tttttttctt gtgtttttaa
     1321 atactcacct aatagatcat agatggggta aagattatat cgatctgctt ttttaccgtt
     1381 gacaatcgtc gtttctatct tgataaatcc atttgttacg agacgatcca gtatttgaaa
     1441 aatctgatct tgtttgattc ccatatccat agcaatcatc tgcaaatcag ggaaagtatc
     1501 tccttcaagc tgtgccatat gcaactgtag aacaaataaa aattcttcag attgtagacc
     1561 gatcctatga taatttttaa gtaaaagatt agatatgact gtttgtccag cgcttaagta
     1621 attttctaaa ttcaacatga tttctcctcc gctcaatcac agtatacgta aaaaaaacat
     1681 gggaagcaag aacaggtttc ttttctattt acttcactga tcggtcagat ttacaatgtc
     1741 gtctacttga tttttgtttg ttcatgtttt tgatgcgtat cttcactttt cggaataaat
     1801 aaattaagta gatttcgctg ttccttagaa tgttgacaag gaaacaaaat tactatacac
     1861 tatttttgtt tccaagttaa cattattgca taaaggagtt tttacatgta cagtttcaca
     1921 aataaacatt ctaaagtttt tggatacgtc tctgtcttat tcactggatt aggatttact
     1981 atcgtcagtc ctgtacttcc gtttttaacg ctgccttatt ctcactccat acatcagcaa
     2041 gctttctata tcactttgct catgtctgtt tatgctctag ctgccttctt atctgccccc
     2101 attctcggta gcttaagtga tcattttggg cgtcatccga tccttattat cagtttatta
     2161 ggatcaagta tcggatattt gctttttggt ttaggaaatt cgatatggat gttgttcctt
     2221 ggtcgtatca ttgaaggatt gacggcagga gaaatctcca ccttatatgc ttactttgcc
     2281 gacatcacgg aacctaatga aagaaccaaa gtttttggat ggatgggtgc tttagtcggt
     2341 ataggaacaa cgctgggtcc tattataggg ggtctattag ctgagttggg gaattctgtt
     2401 cctatattta ttggagcact ctttactttc ttaaatgccg tttatggcta cacatttatg
     2461 ctagaaagcc ttccaataaa aaaacgttca gtagatttaa gtttctccca tgtaagacct
     2521 tttcaccaat tacaacaatt attcaagatt tcatccgtta ttcctttatt gacagcaggt
     2581 tttgctgttt ggttggcagc tggctctctt caatctatct tttctcaatt ttcgattgac
     2641 gcctttcagt ggaaggctgg gttggttggt ttaagcttct ctttgattgg aatcttggat
     2701 actgtctcgc aattgttcat tatgcctcga ttgctcaaaa aattttcaga acagcagatc
     2761 acaagaatag ggatgttcag tgaaatcctc gcctatctat tcatcacatt atctggaatt
     2821 cttctactgc cgattttgtt tttatttggg attatctgtt atggtttcgg tgattctatt
     2881 tttactcctg tattcaatgg ccaactatcc aattctgtct cagataacca gcaaggtctt
     2941 gtaatgggcg gtacccaaag tatacaatct ctatcgcgcg tcatcggccc gctaatcgca
     3001 ggacagcttt atgctgcagc accatttctt ccgacggtat tcggattttt gcttatgatt
     3061 tttgcctatt atcactacaa taaaaaatag tagcttcatt tgctacttcg tataaattct
     3121 ctgtatcgac cgataaaacg aaatactgtg tcaaattctt cttttgacat attttcaaat
     3181 acagtagcat ctcgctgtac ccatttttga tggagttgct cgtgtctatt ccttattttt
     3241 tgtccgattg gcgtcaattg aaaatagatt tcttttttat tctcttcgct ttgataagtg
     3301 tttatgatcc ctctagctat cagttttttt gttagcttac tgatggctcc tctagtcatg
     3361 taactagcat tcgctaattt tgtcacgtta ggatcaggga ttttcccgat atgttcaatc
     3421 acttctagtt catttagagt aaatcctgca aattcttttt ccatttctgg tttattacga
     3481 tgaataatct cattagctaa atccagatat tcttctatta gcagttgtct tttatccatt
     3541 tctacacctt ctttttcttt cattatattg tttttttgtt tcctattcaa caaagataaa
     3601 aatattttgt aggaaaaccc aatctgaaac caagaaa
//
LOCUS       DADVXU010000164         3497 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.171, whole genome shotgun sequence.
ACCESSION   DADVXU010000164 DADVXU010000000
VERSION     DADVXU010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.171"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..2232)
                     /locus_tag="KB953_002783"
     CDS             complement(<1..2232)
                     /locus_tag="KB953_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867689.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="KB953_002784"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="KB953_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          988 a          773 c          549 g         1187 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacatt tttataaaac gacgaaaaat aaatcactac cagtcaactt ctataatatt
     3481 accaaataac aaactaa
//
LOCUS       DADVXU010000165         3443 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.172, whole genome shotgun sequence.
ACCESSION   DADVXU010000165 DADVXU010000000
VERSION     DADVXU010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.172"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..547
                     /locus_tag="KB953_002785"
     CDS             <1..547
                     /locus_tag="KB953_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBB6867690.1"
                     /translation="TSSKKFITACRLDKQKNLPLMIDAIGKLIHEGYECELDIYGEGG
                     LKTELESYINHNGLTEFVHLKEFTNKIHEKMQNAAGFVISSDYEGISNSMLEALAIGV
                     PVIATDCPVGGARMYLGDRNEIGHLVECNNLDKLTDAMRRVLLDRESVVRKAQKGIKL
                     RKELTSKNISNIWYEYLNQFF"
     gene            590..1990
                     /gene="wzy"
                     /locus_tag="KB953_002786"
     CDS             590..1990
                     /gene="wzy"
                     /locus_tag="KB953_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy"
                     /protein_id="HBB6867691.1"
                     /translation="MFSLTLVAFTIFCAFSLNQNYWLNWLGILIYLYAICTWHWRKGD
                     SIFSLYTIFFTLFLLFSYGQCIMWAFGIGTDRGIGTTVVAYGTGIIPSESDMIMVKWY
                     SCISMFVFHIGALLFTRKTTLRKVSWYESGDADADRKFLFKIGCIISIIVVPIVFVSK
                     LLEVRIALVHGYNALYYGDYATQSGYLQIILYLFFPALICLLIGSNFSKVITRFVFII
                     FALYSLLGIMSGDRGSWLYSSIILIWAYTQYKGTFNYKKLIKWLILAVIGIYILNVIT
                     KARDGGGLSKLTLKDFTSVFDSDDSPLVDSFFEMGGTMSIITFFLISGNGIYPFANTY
                     LTSLLGSISTRMLSMFGIKFVLLADWFSQDYLGITWGTGFSMIAEAYVNGGYIGGIVY
                     IFIIGIVIGKILSNSIDNQDIENHPMKMFLAITTANIIMGFPRAASYLIVKNFVYGVL
                     IILLINFFLQQFNKRL"
     gene            2036..>3443
                     /locus_tag="KB953_002787"
     CDS             2036..>3443
                     /locus_tag="KB953_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HBB6867692.1"
                     /translation="MERTARLKKNTIWALVFQITSIVCGMILLRLILTKYGSEVNGMI
                     SSINQFLQIISFLQLGVGAVMQSTLYKPLAEKDNLMISQILASGGKFYRQIAYILLVY
                     VGLLLAVYYVLVPNEFGRSYTMAMIATLSINSFAQYYFGIVNTLLLSADQKIYITYIA
                     QTIAFISNTVACALLISLNTEILMVKLITAIIYLACPIYYRWYVDRNYNIKRNVSYDK
                     EPIPQKWNGVAQHVASVVLDSTDVVVLSIFSNLIYVSIYNVYNLVIYGVKQLFMAMTN
                     GVQALLGELWVRKEIEVLYKTFAWFEWIVHTLTVFIFGCTGILIVPFIQVYTRGVTDA
                     NYNQLSFAALLVTANALHCLRTPYHVMIKAAGKYKETQNSFIIASLLNLGLSLILVKP
                     LGLVGVALGSLISITYHTLWSANYNSKYLLKWPMKKFYKQLFVNILTVVLSFGATSQL
                     KMMSITYLDWIVLAIKCAV"
BASE COUNT         1142 a          442 c          628 g         1231 t
ORIGIN      
        1 tacaagtagt aaaaaattta ttactgcatg tagattggat aaacaaaaaa atttgccctt
       61 aatgattgat gcaataggaa aacttattca tgaaggatat gagtgtgaac tggatattta
      121 tggagaaggg ggattaaaaa ccgagcttga aagttatatc aaccataatg gattaacaga
      181 atttgttcat ctaaaagaat ttacaaataa aatacatgaa aagatgcaaa atgcggcagg
      241 atttgtaatt tcttctgatt atgaaggtat atctaattct atgttagaag cactggctat
      301 tggagtgcca gttatcgcta cagattgtcc agtaggtggc gcaagaatgt atttaggtga
      361 tagaaatgaa ataggtcatc ttgttgaatg taataatcta gataagttaa cagatgcaat
      421 gcgtagggta cttttggata gagaaagtgt ggtaagaaaa gcgcaaaaag gaattaaact
      481 aagaaaagaa ctgacatcta aaaatatttc aaatatatgg tacgaatatt taaaccagtt
      541 cttttaacca aaaggaggat attgtgtgaa agaaaaaata aatatattga tgttttcgtt
      601 aacgttagtc gcgtttacaa tattttgcgc tttttcatta aatcagaact attggctaaa
      661 ttggcttgga atattgattt acttatatgc aatctgtaca tggcattgga gaaaaggaga
      721 ttcaatattt tcattataca cgattttttt cactcttttt ttattattta gttatgggca
      781 atgcattatg tgggcattcg gtattggaac agatcgtgga atcggtacaa cagtagtagc
      841 ttatggtacg gggattattc ctagtgaatc agatatgata atggtaaaat ggtattcttg
      901 tatttctatg tttgtatttc acataggagc actcttattt acgagaaaaa ccacactaag
      961 aaaagttagt tggtatgaat ctggagatgc tgatgccgat agaaagtttt tatttaaaat
     1021 agggtgtatc atttctataa tagtagtacc gatagttttt gtttctaaat tactagaggt
     1081 acgtatagca ttagtccatg gttataatgc attatattat ggtgattatg ctacacaaag
     1141 tggatattta caaattattc tatatctttt ttttccagcc ttaatatgtt tattaattgg
     1201 tagcaacttt tcaaaagtaa taacgagatt tgtttttatt atttttgctt tatattcttt
     1261 attaggaatt atgtcaggag acagaggaag ttggttgtat agctctatta tactcatatg
     1321 ggcatacact caatataaag gaacctttaa ttataaaaaa ttaataaagt ggcttatttt
     1381 agctgttata ggaatatata ttctaaacgt aataacgaaa gcacgtgatg gtggaggact
     1441 aagtaaactt actttaaaag attttacatc tgtatttgat tcagatgatt ctcctttagt
     1501 tgattctttt tttgaaatgg gggggactat gagtattata acttttttcc taataagtgg
     1561 taatggaata tatccctttg ctaatactta tttgacctca ctcttaggct ctatatcaac
     1621 aagaatgcta tcaatgtttg gaataaagtt tgttttatta gctgattggt tttctcagga
     1681 ttatttagga atcacttggg ggactggatt ctcaatgatt gcagaagcct atgtcaatgg
     1741 tggatacatt gggggtatag tctatatttt tattattggt atagtcatcg gtaaaatcct
     1801 ttctaatagt atagacaacc aagatattga aaatcatcct atgaagatgt ttttggctat
     1861 aactacagca aatattataa tgggttttcc tagagctgct tcatatttaa ttgtgaaaaa
     1921 ttttgtgtat ggtgttttaa ttatattgtt gataaacttt ttcctacagc aatttaataa
     1981 acgtctttaa tatttaagct taataaaaag ataaaacaag tcaggagttg caaaaatgga
     2041 aagaactgct cggctcaaaa aaaacactat ttgggctctg gtgtttcaaa taacttctat
     2101 tgtctgtggt atgatcttgc tacgtttgat attaacaaag tacggatccg aggttaatgg
     2161 aatgattagc tctattaacc aatttctaca aattatttct tttttacaat taggagtagg
     2221 cgctgtaatg caatcaactc tatacaaacc actagcagaa aaggataatt taatgataag
     2281 tcaaatctta gcttctggag ggaagtttta tcggcaaatt gcatatattc tactagtata
     2341 cgtgggtttg ttacttgcag tctattatgt tctcgttcct aatgaatttg ggagatcata
     2401 tacaatggca atgattgcaa cattaagtat taattcattt gctcaatatt attttggtat
     2461 tgttaatact ttgttattaa gtgctgacca gaaaatttat attacgtata ttgcacagac
     2521 aatagcattt atttctaata cagttgcatg tgctttatta attagtctga acacagagat
     2581 ccttatggta aaacttatta ctgctataat ttatcttgct tgtccaatat attatagatg
     2641 gtatgtggat cgaaactata atataaagag gaatgtgtca tatgataagg aaccgatccc
     2701 tcaaaaatgg aatggggttg cacaacacgt agcttctgtt gtactagata gtactgatgt
     2761 tgtagtgtta tctatatttt ctaacctgat atatgtatct atatataatg tgtacaattt
     2821 agtaatatac ggagtaaaac agctattcat ggctatgaca aatggcgttc aggccttact
     2881 aggggaactt tgggtcagaa aagaaataga agttctttac aaaacatttg catggttcga
     2941 atggattgta catactttga cagtttttat ttttggctgt accggtatac taatcgttcc
     3001 atttattcaa gtatatacaa gaggagtaac agatgctaat tataatcaat tatcattcgc
     3061 agctttactt gtaactgcta acgcacttca ctgtctgcgt acaccttatc atgtaatgat
     3121 aaaagcagct ggtaaatata aagaaactca aaatagtttt ataattgctt ctttgttaaa
     3181 tttaggttta tcattaattt tggttaaacc actagggcta gttggagtag cattaggatc
     3241 tctaatttca atcacatatc atacactttg gagtgcaaat tataattcaa aatatttgct
     3301 taaatggccg atgaaaaaat tctataaaca gttatttgta aacatcttaa ctgtagttct
     3361 ttcttttggg gcgacatcac aattgaaaat gatgtcaatc acatatttgg attggattgt
     3421 tctcgcaatt aaatgtgcag tta
//
LOCUS       DADVXU010000166         3346 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.173, whole genome shotgun sequence.
ACCESSION   DADVXU010000166 DADVXU010000000
VERSION     DADVXU010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.173"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(451..1761)
                     /locus_tag="KB953_002788"
     CDS             complement(451..1761)
                     /locus_tag="KB953_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HBB6867693.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3191)
                     /locus_tag="KB953_002789"
     CDS             complement(1773..3191)
                     /locus_tag="KB953_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB6867694.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
BASE COUNT         1036 a          677 c          516 g         1117 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt
     3181 tgtgaaagca tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct
     3241 ttttttcttg tttttatatt cacaatgttg gctttttatt gcattatctt gacagataaa
     3301 aaacaaaaaa taatctaaaa acattgatgc ataagattga taactt
//
LOCUS       DADVXU010000167         3300 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.174, whole genome shotgun sequence.
ACCESSION   DADVXU010000167 DADVXU010000000
VERSION     DADVXU010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.174"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(51..2522)
                     /locus_tag="KB953_002790"
     CDS             complement(51..2522)
                     /locus_tag="KB953_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBB6867695.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            2795..>3300
                     /locus_tag="KB953_002791"
     CDS             2795..>3300
                     /locus_tag="KB953_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBB6867696.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQVILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSM"
BASE COUNT          975 a          664 c          595 g         1066 t
ORIGIN      
        1 aagatacacg agtggttgtt cttttttact cttttttgag atacttttcg tcaattttct
       61 cgtttaaata tcgatagaaa ttttttagct acgcgactaa acggcttttg ctctttttca
      121 tttttacgtt caaacaaata ttctaccaat gtggtcaaat gcctaaaaat cgaatctgcc
      181 gagaaaaata gaatgctcaa cagcaaaagt gatacagctg agataccggt gattgaaaac
      241 agcttatgca aaaagatgac cgaaaaaaag ttaccgctag cacagaaaag aatgatctga
      301 cttctgaatt tattcagcgg tgaacagatt ttatagagaa cgaggaaccc tactgcaatc
      361 aaaagcatgg tagatgctgt agaaacatct gttttattta gattcaaagt caccccgcag
      421 atcaccaacg caccgactac gatcatatct gttagtccac ctggcacagc tttttccatc
      481 acattcatga tgaatttccc tctgattcgc tttttgtttt cttctagagc aagcaagaaa
      541 gatggaagtc cgatagtaaa caaactaatc aatgtgattt gtgatggttc taaaggatag
      601 gtcagagcaa agatcactga aaacagtgag agcaaaaagg agaaaatatt cttcactaaa
      661 aacaagctgg ctgatcgctc aatattgttc acaacgcgcc gtccttctgc aacaacttct
      721 ggcatgcgtg aaaagtcaga atcaagtaag accacttgtg aggcctgcat ggcggcttca
      781 tttccggaag ccatcgctat actgcagtct gcttctttca tggctagaat atcattcacg
      841 ccatcacctg tcatagctac tgtattgtta cgctttatca attgaacgaa gattttcttt
      901 tgttctggct tgacgcgtcc aaaaactgta tatttttcga cagctgcttc atattcttct
      961 tctgataatt tagatgcatc aacgtactga tcagcatgtg aaatccctgc ttggacagct
     1021 acattggata cggtgagcgg attatctcca gaaatcactt tgatggctac ttgctgtttt
     1081 ttgaagtaat caaatgtggc tcttgcttct tttctgatag gattagcaat cagaatatag
     1141 cctaatggtg tgactgcttc tgtcaatact ggttcttcaa gaatcccatc gtacacacca
     1201 aagaccaaca cgcgatagcc tttttcagcg aaagaaacaa actcttccgc atattccggc
     1261 aattgatcgc gcaaaaccat ttcgggcgct cctaaaacat acaccttgtc tgccaacgct
     1321 acactgctga atttttggac agaagaaaaa ggaataacat ggtgatagga ttgatcagtg
     1381 gtttttgtaa aatatgcttt aacagcttcc attgttgcat tgtctcctgc catcgctttt
     1441 gcatagtcag caatgcatgc ttccaattga tctgactgac tgattatttc ttgtttttta
     1501 gagacgatca ttttttgaac agacatttta ttttctgtga tcgttcctgt tttatctaca
     1561 caaaggacat ttacccttgc caatgtctca atgcttttca tattgtgtaa aagaactctt
     1621 tgctttgcta gtttagtcgc acttaatgct aatgcaatcg tcgtcagcaa gtacagtcct
     1681 tcaggtatca taccgatcaa tgctgcttcc attgcaacga cactttcttt gatcgtgttt
     1741 ccattaaaaa agtaactttg agaaaaaagc aggataccga taggaataat gatgatccca
     1801 atccatttga tcaattgatt aagagaagca atcatctccg actgttctcc ttctcccatt
     1861 gtcttggctt tgactgtcag ctgggaaata tacgaatctg ctccaacttt gtctaatcgg
     1921 gcgtaagctt ttccggaaac aatgaaactg ccggacatta atggatcacc tatttctttg
     1981 gttatttcat ctgcttcgcc tgtcaataaa gcctcattaa cttgcagttt cccttccacg
     2041 acctctgcat ctgctggaat ttgatatcca gtctctaaaa taacaatgtc atctattaca
     2101 aggtcttcta tatctattgt ttgttctatc ccgtctcgta tgacattgac ttttgctgta
     2161 tttaaaacat ttagttcgtc caagatcttt ttggatcgaa tttcctgaaa aatggcgatt
     2221 cctgtattgg ctatgataac tggtaaaaaa gtcaagttct tataggattc tactaaacaa
     2281 agcaaaatgg ctaagataag aaagatcaaa ttgaaatacg taaatatgtt ttccaatacg
     2341 atttgctgat ttgttttgaa tgtgggatcg actgaattat ttgctgcacc tttttctatt
     2401 cgttccttga cttgatctgc cgttaatcct tctttatacg atggaaaaag acggatgact
     2461 ttatctgatg atctttgtgt tactttcttt tttttacttc gatttatcca tttattcttc
     2521 atgtggctac ctcgattgat ttcgttttct ggatatttcc aaacttctcc tatccagtat
     2581 agaacagata tggaaagtgt tcctcacatt aagcttgcaa acacctttca ttcctgtcat
     2641 atagcaaaaa aaaagggttc tataatattt tgtgttggtg gaaaatcgaa ggcgattttc
     2701 tactaacact ttttcttcgt ttcaactcaa aaagagactc acttttggta aaatttagtc
     2761 gaccaaaacg tcatcctacc aaaggagtct ctcaatggat aatcatacta gaaaattact
     2821 cggattaaca gataaacatt tattttttga tgaagaatgg ctgattgaaa aagaatataa
     2881 aggcgttcaa gcgcagttta ttaaaggaaa attggatgac tcttcttcct attgtgaaca
     2941 ctgtcgatcg gttcgtatta tccgcaacgg ttcctatacg acacgcactc agatgttgaa
     3001 agtcaaagaa aagttaacta ttttagaatt aaaacgtacc cgttttctct gccatgattg
     3061 tggaaggacc ttttctgcga aaacggactt agttgatgaa catcatcaac taacaaagga
     3121 actgaagcag gtgattctca tggaactgta cgaaaatcaa tcacgtaaac taatcgctaa
     3181 gaagtacttc gtctcggatg ggacggtgac acggatcttg cgtgaagcaa cgaagcatta
     3241 ccagccacgt atgaacttcc ttccgacggt gttgtgtatg gatgagttca aatcaatgaa
//
LOCUS       DADVXU010000168         3283 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.175, whole genome shotgun sequence.
ACCESSION   DADVXU010000168 DADVXU010000000
VERSION     DADVXU010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.175"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..125)
                     /locus_tag="KB953_002792"
     CDS             complement(<1..125)
                     /locus_tag="KB953_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HBB6867697.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYG"
     gene            complement(143..463)
                     /locus_tag="KB953_002793"
     CDS             complement(143..463)
                     /locus_tag="KB953_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6867698.1"
                     /translation="MITFERIKELAKKQGKSLNKVEEDLGYGKNVLYRLKNSNPSTER
                     LQEIADYFDVSVDYLLGREEKETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            757..897
                     /locus_tag="KB953_002794"
     CDS             757..897
                     /locus_tag="KB953_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867699.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
     gene            1195..1452
                     /locus_tag="KB953_002795"
     CDS             1195..1452
                     /locus_tag="KB953_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HBB6867700.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(1423..1731)
                     /locus_tag="KB953_002796"
     CDS             complement(1423..1731)
                     /locus_tag="KB953_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867701.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            1809..2555
                     /locus_tag="KB953_002797"
     CDS             1809..2555
                     /locus_tag="KB953_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HBB6867702.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            2570..2773
                     /locus_tag="KB953_002798"
     CDS             2570..2773
                     /locus_tag="KB953_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6867703.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            2878..3177
                     /locus_tag="KB953_002799"
     CDS             2878..3177
                     /locus_tag="KB953_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867704.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKGLSPSDVLKEAS"
BASE COUNT         1177 a          571 c          582 g          953 t
ORIGIN      
        1 ccatatagat tttcattcaa aaaaatgatt ctaggcttcg gataatagaa gccaggctct
       61 ttcatttcca tgtaaactac tttcaaattg tattcgctca acatttcttt caatttcaaa
      121 tacatacaac ccatcactcc aactattcat tttcctctaa agctttagca attgcaatcg
      181 ctttacgcat tgtctcctta gatatttctt ttccatcaaa agaaaaaaca gtatcgtttt
      241 ctgataaatc cacatgttta ggggtttctt tttcttcgcg ccccagaaga tagtctacag
      301 agacatcgaa atagtcagca atttcttgca aacgttctgt agaggggttt gagttcttaa
      361 gtctatataa gacgttttta ccatagccta ggtcttcttc aactttattt aatgattttc
      421 cctgtttttt cgctaactct tttattctct caaacgttat catatcaata ttcctttcgt
      481 tcaagaaaaa atatttaact ttatttgtta aaattcgttg actattttaa caaaaggtgt
      541 taatatcatt cttgtaaaca agtttaacaa ctaaaaagac aacaaaaaac actattgatt
      601 aataaatgcc aaccgccaag aaagctttta aaatcaatgt ttatatgtct tatttaacta
      661 tgttctgatt ttaacactgt atgttaattt agtcaacgca gttttttaaa aagttgttaa
      721 atttgtttac gaatataaaa gaaaggagag aagaatatgg aaaaaacagt ctcggcaaaa
      781 atcgaagatt tgaaattaga catactgaaa caagcaaaag tggcgatgga acacgcggta
      841 gataaagaag actctgccat ggttgcagcc atagcagaga ttttagctca cgtttagtca
      901 tcgtttttac ggtcttctgg aagctgacca tagataacag cataatgaat atctaaataa
      961 gcttgaacaa tttcttttgg cgaaattgat tcgcctttag tagatacttg tgattcgtga
     1021 tttagccatg caacaactaa atccgcagcg atttcaaacg gcgttacact ttgtgtcagt
     1081 tgccataaag aatttcacaa tatgtatggt cgtggtaata ataccaaaga gcaatttgag
     1141 gaatttatgg aaattaaaaa atacaaaaaa tcatttatta aaggaggcta aacaatgaac
     1201 aaggaaatga gccaaacaat caaggtacaa aaaatgatag atgatctaac acatggaatt
     1261 gatagccaag ccgataaaat catcaaggaa ttgcaaggac aaaaagttaa ggatgcaaaa
     1321 atgcttctca agactatcaa ctttgaaatg aatccaacaa aaagaaagct cgccgatgtg
     1381 ttggaagaaa agttggcttc cgctatcaac gagcaagaat tactatttga aaccgatact
     1441 tttaacagct gatgttttat agatgacagt ttcatccgaa tctatatcat cgccgagaga
     1501 aaagaatgtt gcttttgaca aaaagccgat aagacctaca agcggtcttg atgttgcaag
     1561 agtagatacc ccaatatcac cttgcaaggt attcacgtaa atttgagaag aatagaacat
     1621 tccatctttt tcttcgatat ttgacgcatc atcgtatcca acgaccaaag tatcggcaaa
     1681 tattttgatt ttttctccat caagcatagt aattacaaat tctggtttca taaattttca
     1741 cctccttaac aattatttca gcctgtcaca ctgataagga aattatacca aagaaaggaa
     1801 tgagaaatat gaatacacca caaattttta atttcgagca aaacgaagtt cgaactattt
     1861 tagtaaatga tgaaccatat tttgtaggaa aagatgtagc agacgttcta ggatattcga
     1921 atccgcaaaa agctattcga gatcatgtgg acttggagga taagacgcag aacgattcgt
     1981 tcaccgtcaa tggaacagca gttgttttga ttaacgagtc aggcctttac agtttaatct
     2041 taaaatcaaa acttccctct gccaaaaaat ttaaacgttg ggtaacgagt gaagtccttc
     2101 caacaattag aaaaacaggt agctattcaa acgtacctca aagttttgca caagcattgc
     2161 gtttagcagc ggatttagaa gaaaagaacc aattactcga acaacaaatt gccgagtacg
     2221 aaccaaagat tagctactta gatacgattc tttcatcgac agatacggta gctacttctc
     2281 aaattgcagc tgattacgga atgtcggcaa ttgctctaaa caaattgcta aacgagttag
     2341 gtgttcagca taaagttagc ggacaatgga tactttaccg aaaacatatg aaccaaggat
     2401 acacaaaatc gcacacaagt gagataccga aagccgatgg cggcactaaa gttgtaatga
     2461 ataccaaatg gacacagaaa gggcgagtgt ttatttacaa cttattaatc gcagagggct
     2521 attaccctca aatggattta gaggaaattg gttagaaagg agttttagta tgactgacat
     2581 tgcagaaatc actcaacgag atagagaaaa aatcaaagaa tatgtcgaaa gttcaaagtt
     2641 cttaacttac accatgcttg ctgaaagatt tggaattagc aaaagctact tatctttaat
     2701 tttaaacggt aaaaagactt ctgcagaagc aaacagaatt atagattcga ttatcactat
     2761 gtacgaattg tagagggagg taaagtcgtt ggaagataaa atcattctta caaaatcaga
     2821 gcttcaagaa atgttaaaca tcgagtatgg aacgataatt tttaggaggg ataataagtg
     2881 aaaaaaccaa cactttcaga gctgatagaa gctgctgaga aggcagtaaa accagacgac
     2941 tggtaccgac aaagtttaat cttggagaag ttccacggca tgtcaaaaac tactttagtt
     3001 gaatactgca aggaaatgga aacaattcct gaatttgcag aaggaatact tcgtccaggt
     3061 cactcaacca catttattca ttaccatact tttatttggt ttttaaagtg gaaagacgca
     3121 aacaaatatc gtgtaaaagg tttatcgcct tcagatgttt tgaaggaggc aagctaatgg
     3181 gcaaattcaa cagagcgcta gtattcagca caccattcat catctacgct ttaggtcttt
     3241 ggggaagcag acaagcatta atcggcacaa tcgtatatat ggt
//
LOCUS       DADVXU010000169         3283 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.176, whole genome shotgun sequence.
ACCESSION   DADVXU010000169 DADVXU010000000
VERSION     DADVXU010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.176"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            63..878
                     /locus_tag="KB953_002800"
     CDS             63..878
                     /locus_tag="KB953_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867705.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            1004..1261
                     /locus_tag="KB953_002801"
     CDS             1004..1261
                     /locus_tag="KB953_002801"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867706.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(1513..2085)
                     /locus_tag="KB953_002802"
     CDS             complement(1513..2085)
                     /locus_tag="KB953_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBB6867707.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2101..2706)
                     /locus_tag="KB953_002803"
     CDS             complement(2101..2706)
                     /locus_tag="KB953_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HBB6867708.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            complement(3107..>3283)
                     /locus_tag="KB953_002804"
     CDS             complement(3107..>3283)
                     /locus_tag="KB953_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6867709.1"
                     /translation="YLLGMALAYSVGSLIKVNVSLDLFTILLAVGVSSVIGLVFSVMP
                     ASEAAKKDLIDILR"
BASE COUNT         1078 a          611 c          501 g         1093 t
ORIGIN      
        1 tgctggacta caagtaaaaa atactgtatg atcatgacag attcaagaaa ggagcaataa
       61 gattggttaa gatagcagga ctttctaaag gtggaaaaac cgtttcacaa ctttataaac
      121 aaaactacag agactggaga cagttcagaa aaaacattaa ggattcttac tttattctac
      181 caacagaact tgaaaaatat ttaccgaata acaaagctat taatttatat ctgtattact
      241 gttttaaggc aaagaatgaa ggcggggatt cctggcattc ggtcaatacc atagcagagt
      301 ctctaggagt gacaactaaa actataaaca cctggaataa aaccttagtt gatttaggta
      361 tgattgcacg tatagacgat aaccatctca gtaaatcgac aattttactt cctctctcta
      421 gttactataa attagccctt gatagttctt taaaggatgt taccgagagc actgtccatg
      481 aaattgatgg caacttggtt gcagtttatc atttgtttga atggagaaaa aatgaggctg
      541 atgaaaatta taacgttcct tataacacac tctgttgtgt ttttaaaaga aaaacacaaa
      601 aagatactat ctataaattt atcttggtcc aagatgagaa aataagtaat ttcgaattaa
      661 gcactccttc tagtaagatt tatgacgatg catatagatt tgaatgtcaa gatctagaag
      721 ccctcctttc cgcaaataat ctaaacaata tagaacatga aaactttgtt gtaaacacta
      781 gatttaattt agtttctgaa aaggactcag atttgttaga tttgctgata gagcttacta
      841 aaaatctaga taagaaagaa aacattactg tactataact agcatcggtc catcattaag
      901 tttggactat tagaacactt gctattacag aaaaaggtat gctagccacg cttactattt
      961 attggtatca aacaagtatc ttaatttgaa aggaagatga tttatggcta atttatatat
     1021 agagaacaaa ggagacttga atgccattat tactttaatc gtagttatgg taaaatcaat
     1081 aggtaatatc acaataggaa tgctgtttat tatattagca gcagtgctga tttttatgat
     1141 agttatttgg ttcttaaatg aaagatctaa agacaatcaa caccgtaggg atatggagat
     1201 aaaatcaata gagtcaatag attatttatc gataataaca ctaaaaaagc cacgttccta
     1261 aacgtgactt acgctgaacg atatgtgttg tttggtagac gaacaaatcg ttcattataa
     1321 atatatgatg gtatcctaat atagtaagcg tggctagcat acctttaatt actttacctt
     1381 ttacagttac catacacatt ttttgagcag aaatcaagta aataaaaaaa tattatattt
     1441 cacaacatgc taatagactc atatctagaa cgcattaagt atttacctgt ctggttcgct
     1501 gagacttctt ttttaacttt cttcaatttt cttctttatt ctaaaaagcg tactgcgact
     1561 gaaaccggtt atagcttcaa cttccttata cgatttacct gaagtaagca gttcatatgc
     1621 gtgcctcttc ttcggtgtga ttgttgcttt cggacgtcct tctctgaaat taggattatt
     1681 ctttcttgca aacatttttc cttcttgggt acgttccaca atcatatcac gttccatttc
     1741 tgctacactc aacaacgtcc gtgtcaccat tcgccccatg gacgtatttt caatcgttcc
     1801 tagattcaaa accttgatcg ttacgttatc atcgagtaaa ggttcaatga tctctaatgc
     1861 ttctctcgtg ttacgagcaa atcgatcaag cttcgtaaca actatttcat ctccaggctt
     1921 cagaccagcc aacagctgat tgaattttgg ccgatcccgt tttgtacccg taaatttttc
     1981 tgaaaaaata ttcttcttct ctacgccgtt ctcaagcaac tgttgaattt gttcttttgt
     2041 actttgagaa gcggttgaga cacgtgcata tccatatttc gtcatttggt ccacctactt
     2101 ttacaaatca tctatcgtat actcagaata cccttcatag aaatagctga catcgattcc
     2161 tttcatatat aactcgcgat cgtcaatttt atcagtcaga gcattggaaa ttagatagcg
     2221 tatttccaaa tcgtttatgg gacttcgctc cattgcagaa agatagtctg ctttatcgat
     2281 taagttccaa tctacaactt tttgcaattc ttcctttaaa atgagatcca gccaaattct
     2341 agtacttcgg ccatttcctt ctcgaaatgg gtgagcgata ttcatctcca catatttctt
     2401 gataatctct tcaaaagaag tttgtggcat ctgatcaata tgctgtaacg aatgttctaa
     2461 atacataact ggtgcaaatc gaaaatttcc ttttgcaata ttgaccgatc ggatttttcc
     2521 ggcaaaatcg taaatatccg aaaatagaaa ttcatgaatt tcagataaac ctttaaatgt
     2581 accaacttcg atttcattta tttttccaca atcgtacaat tctttcgctt tttgtttact
     2641 gagtttttct tcaaccttag ccaactcaac ttgattggtt aatcccaatt tattctctaa
     2701 aatcatgcga aatctcctta actttatgac ataagtttat gaaacactct caaccccatt
     2761 atatcgcgat tttgtttatg tgtcaaaaac tattaagttt ctgacccccg atagattttt
     2821 caagtgtctc tattttgcac agtctagttg acctttgata cacctctata tttatagaga
     2881 atctaaaaca tcaacatggc atactcaaaa ttctaaatat tagggataaa gaattctcct
     2941 atacaatcaa aaatttcctt tatttcgaac tccggtcacc taactctatt gatctcttac
     3001 aagccaaaga atatctctat actacaaaga aacaataggg tgcctggttg gtctctttgt
     3061 gttttatttt agaatctaga acgtaaatat tcttgtcatt ctgattttat ctaagaatat
     3121 caattaaatc tttttttgca gcctctgaag cgggcattac agaaaagacc agtccaataa
     3181 ccgaagatac cccaaccgct agtaaaatag taaatagatc aagtgacacg tttaccttta
     3241 tcaaagaacc aacactataa gcaagagcca ttcctaataa ata
//
LOCUS       DADVXU010000170         3193 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.177, whole genome shotgun sequence.
ACCESSION   DADVXU010000170 DADVXU010000000
VERSION     DADVXU010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.177"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..250
                     /locus_tag="KB953_002805"
     CDS             <1..250
                     /locus_tag="KB953_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867710.1"
                     /translation="ARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFMDYQK
                     LDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            867..>3193
                     /gene="esp"
                     /locus_tag="KB953_002806"
     CDS             867..>3193
                     /gene="esp"
                     /locus_tag="KB953_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HBB6867711.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT         1170 a          471 c          614 g          938 t
ORIGIN      
        1 agctagagaa tctatcagtc atattaatgg tagcaatttt aaacaaacat tatttaatct
       61 gattaagcta gatgctaaaa tatcaagtta tttattcttt ttgatacatg atgaatttat
      121 ggattatcaa aaattggatc agttaattga aagagaaagc tgggaagatt attacgtggg
      181 tctaactttt tcagaaaagt taaacaacta caagttaata gattataagt atttgagtga
      241 aagcaattaa ttattcatgt agagtactag acactttaaa tatggacaat tcaattataa
      301 agaaaaagaa tcaataattt aatgaggctg tgacaaatgt cattttttgt ttcgctaaaa
      361 acgagtgaaa aaatgttgat aactccaatc attcgtttaa gattctagga atcattcaga
      421 aatgctgtga aaccgatact ttataattat aaagaaggtg atttcaaatt ccgaagaagg
      481 aatttgaagt caccttttta cttatgtcac agtctcattc agtaatgcgg gataaggctc
      541 cttattccgt tatttgaata ctctgaagtt taatctaaga aagggtaata ttttgaaaac
      601 atgcttcatc gcatagaaat gaacttaatt tcagaaagaa tacatacata tctttttata
      661 tgttttcagc tgctgaatgg ttaaaaagca caaatatccg aaaaaaacat gaagaaatgt
      721 actttttttg ttgtaatgtt catgaaattc tctaagtaaa ttagttaaca taaggttagt
      781 aagattccta ttctgtgaat aatacgtatc agatttatct tttattctta ctaggaaagt
      841 aattattaag ggagttgatt tgaataatgg ttagcaagaa taataagaga gtatttctag
      901 aaaaaacaaa gaaacgagta ttgaagtaca gtatcaaaaa attaagtgtt ggggttgcat
      961 ctgttttgat tggtgttggt cttgtcttgg gaacaactga actagttaaa gcacaagatg
     1021 aaatttctcc aagcagccca cttgaaacag cgataagctc cgtacaagta ggtgacaaag
     1081 tagcttcggg gaatactttt caggagaatc ctggttatac gaaaaattat aacttttcgg
     1141 atttacaatt tagtcctcaa gaactaactg gagacacact aaaaggcaat acaattggat
     1201 ttgaagttta cggaaaacat aatattgcag catcaactaa aaactgggaa atccgtcttc
     1261 aattagatga acgattagct aaatatgtag aaaaaattca ggtcgatcca aagaaaggaa
     1321 ttggatcttc tagacggacc tttgtaagaa ttaatgattc gctgggtaga cctacaaata
     1381 tttggaaggt caattatatt cgtgcaagtg atggattatt tgctggggca gaaacaaccg
     1441 atacgcaaac cgctcctaat ggggtaatta cgtttgaaaa gagcctagat gaaattttta
     1501 aagagatagg catagacaat ctcaagaccg atcgtttaat gtatcgaatc tacttggtaa
     1561 gtcatcaaga cgatgataaa attgtaccgg gtattgatag tacgggctac tttttaacag
     1621 attcagatga tttctataat agcttagatg tatctgaaaa taatcctgac cagtttaaac
     1681 atggatcagt aagtgcaaaa tatgagaaac ctaatactca aacaaaagat ggttcaggat
     1741 ccacaggtgc taatggagct ataatcttag atcataagtt aacgaaaaat tataatttct
     1801 catattcatc ttccgcaaag ggcacgcctt ggtatgctaa ttacaaaatt gatgaacgat
     1861 tagttccata tgtagcgggt atacagatgc acatggttca ggctgacaaa gtaacatatg
     1921 atgtttcgtt tgaatccgga acaaaagtag ctgatttagc aatcgagcga cgggaaggtc
     1981 atgaaaatta tggcataggt tcaataacta ataatgattt aactaaactt atcgattttg
     2041 ctaatgcaag tccacgtcca gtcgttattc gatatgtttt acaattgact aagccattag
     2101 atgaaatctt agaagatatg aaagcaacag cacaagttga agaaaataaa ccatttggtg
     2161 aagatttcat ctttgattct tggttgtcgg atacgaataa aaaattaatc cagaacactt
     2221 atggaacagg ttattattat ttgcaagata ttgatggtga tggaaaccct gacgataaag
     2281 aagagagcgg agacacgaat ccatatatcg ggaaacctga attagaagaa gtatatgatg
     2341 ttgacacaac agttaagggg aaagtattca tccacgagtt agcgggaaca ggtcacaaag
     2401 cccaacttgt tgataaagaa ggtactgtat tagcagaaaa aactatcgct ccaaatgaaa
     2461 aagatggggc tccaatttca gatactgtag aatttgaatt tacgggtgta gattcaagta
     2521 aattaatcgc gaaagatgaa ttaaaaatcc aaatcgtttc tccaggtttt gataaaccag
     2581 aagaaggttc aaccgttatt aaggaatcac caaaagcggt tgataaacaa accgtggtag
     2641 ttggatttaa accagatgct aaagaatcaa ttcggaataa taaaaactta cctgaagatg
     2701 cagagtattc atggaaaaca gagcctgata cttctaacgt tactgatagt acgaaaggta
     2761 ttgtaactgt taagatcgga aatcgaactt tcgacgtgga tgtagagttt gctgtaaaag
     2821 cttctcaagc tatggaaaat gatgcaacat acgtacctat aacaacaact ccagaaacga
     2881 cagttcaaag tggaaaacct acatttgata aaccagatgt tcctttagct aaagatgcct
     2941 tttcaatttc agatgtctat aataagtact tcggtaatgc aagtgttgat gcaaacactg
     3001 gtattgttac attcactcca gctaaaggtg taggagaatc ggagccgatt actggaacaa
     3061 ttcctattaa aattgtttac caagatggtt ctgtaggcac gaccgattta gcagtaactg
     3121 taagtaaaga tatttatgaa aatccaggag aaaacattcc tgcaggctac cacaaagtaa
     3181 ccttcaccgc agg
//
LOCUS       DADVXU010000171         2984 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.178, whole genome shotgun sequence.
ACCESSION   DADVXU010000171 DADVXU010000000
VERSION     DADVXU010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.178"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(74..616)
                     /locus_tag="KB953_002807"
     CDS             complement(74..616)
                     /locus_tag="KB953_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HBB6867712.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            751..1140
                     /locus_tag="KB953_002808"
     CDS             751..1140
                     /locus_tag="KB953_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867713.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1144..1908
                     /locus_tag="KB953_002809"
     CDS             1144..1908
                     /locus_tag="KB953_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBB6867714.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIQLMEEASTFLAKNLIKKYK"
     gene            2037..2984
                     /locus_tag="KB953_002810"
     CDS             2037..2984
                     /locus_tag="KB953_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBB6867715.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
BASE COUNT          961 a          500 c          613 g          910 t
ORIGIN      
        1 tcaaaataat aaataaaaac tgtctaacat ttaaaattta atttagatat tagacagttt
       61 ttaagtttgt gagttatttt ccttgtcgtt ttctcacttt tttagtccag aaaccacctg
      121 aaatatattt tggctcataa gaaatgataa aagcacgctc ttcgatttct ttgatcaggc
      181 gatacagttc acgttcgctt tttctaggag aaagtatttc gagaactagc cgctcacctt
      241 ccagccccat tgcagaagtc tgtgtcacac cataaccgtg ttctctcagt atacccggca
      301 aacttttatc ttctgcgcta tttgctggca aaatcgttgt gaccatgatg tatcctaatg
      361 ccaatttgtc ttcaatcaaa attccgacac tgatacctac tgcgtaacct aacgcataga
      421 caaataaatt cagtggatta tctaaacgat ctaaaacaag ggataagccg acgatataaa
      481 tcgtgatctc gaccatactg acaagtggag caatcagtcg atagcctttc attgtcaaca
      541 tgaaacgtaa cgtgttcaga gtgatataag caaaattaat gaggaagatc atccccaaca
      601 tttttggatc aatcatttgt aaaactcctt tcatttatat aacaacaata tcaaaaaaaa
      661 tcgtaagtga agaaaaattg aaactttata catacaaaaa aatgataaaa tgattataat
      721 agaattataa agataggtta ggtgattaaa atgaaaacgg tcaatcgtgt gttttgtgcc
      781 agccacgtgc taagtctcct tctcagtata tacttaggtg tctattcgat tcaacagcca
      841 attgaggtag agaacattgt ttttcagatg tcgctgacag atggtttaat tttaagcgtt
      901 tttttctttt tgatactgtt tattggtaat attttcggta gcgttgccag ttttttgaat
      961 ttttctattt atccactcct ttcgctagca ttaggagtgg tgggactttg ttctctgtgc
     1021 ttatttcccg agccattcag tcctgcattt atattatttg gtacactgaa cctgttccag
     1081 acaactgttg gagcatggtt gctatggcga tcaggaaatt taatgaaaat aggtgagtaa
     1141 caattgaatc caattatatt gaaacgttct gtccatcata ttccagtgtt agaagttgtt
     1201 ctgaaagaat taagagagga acgtattccc gttgtcatct attatcacgg atggcagaca
     1261 agtaaggagt tagttttgac gcaaggaaga aaaattgcgc agcagggcat tcgtgtgatc
     1321 ctgccagatg cgatgaatca tggggagcgc aaacagccgg tttcttctat tccatcctat
     1381 acattttggg atagtatcta cggaaatctg tttgaatttg acacacttat caagcattta
     1441 gaaaaaagag gtcttctaca tgatcagctt gcagttggcg gggtttcgat ggggggaatg
     1501 acaacatgcg ccttgttggc taaacatcct catatagtag cgggcgtgtg tttgatgggt
     1561 tcccctgcac ctttggaata tggaaaagaa atcgtaaggc gcgcatcgga atataattat
     1621 gtcgtaccaa aagactattt tgatttgatt tcatgggtta agagctatga tttgtcattg
     1681 cagccggaaa agttagctgg acgtcctctg ttcttttggc atggacaagc tgacgaaaaa
     1741 atcccttata tacaaacgga acgatttgta gaaaaaaatc ggcataagtc ttacggaaaa
     1801 gaaattgttt ttcgttcctc aaaaacacag cgacatatgg tacaaataca actaatggaa
     1861 gaagcttcca cttttttagc aaaaaatttg ataaaaaagt ataaataatt gaaacggaaa
     1921 atcactcatg acgatagagg attttccgtt ttttattttg ggctataata aactctgtta
     1981 ggaaaatttg gtataattga tagtaagaat gacaacatgt tagaaaggaa gagagcatgc
     2041 ccactttagc agatgttgca aaacgtgcca atgtttcaaa aatgacggtc tctcgagtca
     2101 tcaatcatcc ggaacaggtg actgacgaat tgaaagagtt agttttctca gcaatggctg
     2161 aactggatta ccgtccaaat attgctgcaa aagcacttgt cacgaatcgg tcgcagatca
     2221 tcaaattatt tatactggaa gaaatcgaca caactgagcc ctattacatg aatttgctga
     2281 tgggaatcgc taaacggatc ggtaaagcgc attattctct ccaattagtt acaaatgatg
     2341 ccttcgacgt cggttcttgt gatggataca tcatcacagg tgtcagaaaa aaagacttcg
     2401 attggatcga ccgtttggaa aaaccagttg tcttgtttgg ggaaaattct gaaggatatg
     2461 attatgtaga cagcgataat gaatatggta cagcaaaagc aacgcaatat gccttagaaa
     2521 gagattatca aacgattatt tatatcggta tcgaagtgga cgagcctttt gaaatttcaa
     2581 gggaaactgg ttatttatct gtgatgaagg aagttgatct tcctagtaaa atcttgcggt
     2641 ttccaaatca ttcgacagaa gtagaaagat atatgacaga aaactgggca gattttacag
     2701 ataaaacttg ttttgtatgt agttcagatc ggctagcact aggagcagta agagggatta
     2761 taaatgctgg aggacacttg cccgaagaat ttggtgtgat tggatttgat ggggtatttt
     2821 tagaccaaat cagttcacct aaattgacaa cgatcaagca agatattata cgtatgggtg
     2881 aagtatgtgg agaaatgttg ttaaaaaaaa tagaagaaaa aggggcgagt caaggttatc
     2941 gtcgtttttt tcctgaacta gttatcagag agacaacgaa ttaa
//
LOCUS       DADVXU010000172         2962 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.179, whole genome shotgun sequence.
ACCESSION   DADVXU010000172 DADVXU010000000
VERSION     DADVXU010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.179"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..1097)
                     /locus_tag="KB953_002811"
     CDS             complement(<1..1097)
                     /locus_tag="KB953_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBB6867716.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVRGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENA"
     gene            complement(1227..2603)
                     /locus_tag="KB953_002812"
     CDS             complement(1227..2603)
                     /locus_tag="KB953_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6867717.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2603..>2962)
                     /locus_tag="KB953_002813"
     CDS             complement(2603..>2962)
                     /locus_tag="KB953_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBB6867718.1"
                     /translation="NIRLVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARA
                     LVSDAPIILADEPTGNLDSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILE
                     LSGKKLKKVNKMNLEVE"
BASE COUNT          901 a          566 c          413 g         1082 t
ORIGIN      
        1 gcattctcat tagagaacac tccctttttt gtgacttttc tgaagctaga gtgaatactt
       61 tcaactgcat tagtagtgta catgattttt ctaatggcgc tgccataatc aaagagttgt
      121 tcaacatgag aaaagtttcg tttgcataca tctactgcac cagaataatg agaccaccga
      181 ttttgaaaac tgtcgaaggc agcgtgtgca gcgtttaaag aagaagcact gtagaatttt
      241 ttcatgtctc gacagacttc cttataatcc ttacttggga ggtatcgtaa cgcatttcga
      301 acaagatgaa caatacagcg ctgcacaatg acagaaggaa aaattgcttt tgcgccttct
      361 ttgagaccgg aaacaccgtc catcgaaatg aaaaaaacat cttctacacc acgtactttc
      421 agttcatcaa atacttgcat ccagcgattt ttagattctg tttgattgag ccataggcct
      481 aaaatctcct tgtttccttt aagatcatag ccaagaatgg tgtacacagc atattcttta
      541 gcttcatagt tttcgcgtaa agtgacgtac atacaatcaa cgaatagaaa tgcataacac
      601 ttggctaatg gccgagcttg ccattcttcc aattcaggaa ggacggcatc cgtgatatca
      661 gaaatcattt cgtgggaaat atcaaagccg taaatatctt caacggttgc ggcgatatcg
      721 cattgactca ttcctcgtgc atacatggaa agaaccttac cttcgatgtc ggagacatct
      781 cgttttctat taggaatcaa ctcaggttca aaagaagcct cccggtctct gggaacatca
      841 atgtttactt taccaaagct cgttttaagc gtcttattgc cataaccgtt tcgtcgattg
      901 tcatgtttct ttggctcttt agaatgggcg tcatacccta aatggttatt caattctcct
      961 tgaagcattt tttcaaataa gggaccaaac acatctttta aaacatcttg catatcctct
     1021 acagattcgg gttgataagt attcaaaatg gattcagcta acttctcagc atcaggattt
     1081 cttttctttc tagccatcgt gtaatcacct cttgatctta ttttagaaaa ataaaaaccg
     1141 tgaagcaacc acccgcctag gattgatggt tacttacacg gtttatatta cactctcatt
     1201 tttggtgcta aaaagcaccg aaaattttaa ttattgtcaa ttaataattc ttttggattc
     1261 cttcttaata tattgaatga agaaataatc aatgatacaa gaagtactaa actcataaat
     1321 ataactacat aaatcatgaa tttaggtaat acattgatat ctaaacttga taatgtcttg
     1381 ttaaatcctt cagcttctgc cccaccacct aattggctag atgcagattg tttagctatt
     1441 tgtttagcta tatcaccagt aaccttattc aatatattat tcccaagctt gtcagctgta
     1501 tattgagcta agaaatagga accaacgaat gcggggattg atataaatac catctcaatt
     1561 aggaattgac caaaaatttc taattttgat atacctaatg atagtaatac tgctatttct
     1621 ttctttctag cattcatcca taagaataat aataatgaaa ctacaactcc tgcaaatatt
     1681 aatgagccaa aaaataattt atttgaaatt gagtaaatac ctgatataga ttgttgtaat
     1741 gcagggtaat ttgatgaact tttaatcaaa ttatattctc tccaatttat atctaatttc
     1801 ccaagatctt ttattacact atcaagattc ttatcacctt ttacaaagaa tgttgcatct
     1861 tggtatacag ctgtatcttc ggtattacca taaactttag cagctgtatg aatatcagtg
     1921 attaatgtat tttcgtatag ttcttgtgct gcacttactc caccgctatt atgcccatcg
     1981 aatagacctt taatttctac ttctactgtt tcatctgcac ctttctcatt atcagcatca
     2041 aataaattag attttatttt tatcttatct ccaactttaa ggttattttt ttcagctaaa
     2101 tctttatgca ttaagatttt atttttatct tcattttcta aatgctttcc ttctactaat
     2161 ttatatgctt ctgatacaaa ttttgtttct tttgttgagt cgttaacccc agttaacata
     2221 actgttcttt taaaattctt tgctctttca ggtgattgat tcgcaagagt ctcttgagtt
     2281 tcaataatat cataatcaac taaatctgca acactgttta ttctttttac ataggagtct
     2341 atactatctg tttgagatat ctttttaata tcttcacctt ttacatttcc cccacctcta
     2401 ggtgttcctg gatttacttg tctatttatc tccatagaaa aactatttgt tatattagca
     2461 aatgtttctt ttgaagctct gtccgtagca tctttaatag ataaactaat gagacttaaa
     2521 gttgacatag ataaaataac taatatgata attaatgatt ttaaactttt tctagttaca
     2581 taagcaaatg cattttttat cattattcaa cctccaaatt cattttattt acttttttca
     2641 actttttacc acttagttct aaaatgatat ccgcagaatc tgctacttcc ttactatgtg
     2701 ttacaactat tacacattta tttctatctt tagctaatgt ctttaatata ttaattattt
     2761 ctccagcagt aacactgtct agattaccag taggctcatc agctagtatt attggagcat
     2821 ctgataccaa tgctctagca atagctactc tttgttgctg tccaccagat aatttcataa
     2881 catttctttt tatttgtttt ttatctaaac ctagttcaaa caagatactt tcatctgctg
     2941 atttatttac taatctaata tt
//
LOCUS       DADVXU010000173         2948 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.180, whole genome shotgun sequence.
ACCESSION   DADVXU010000173 DADVXU010000000
VERSION     DADVXU010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.180"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            98..2539
                     /locus_tag="KB953_002814"
     CDS             98..2539
                     /locus_tag="KB953_002814"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HBB6867719.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2696..>2948)
                     /locus_tag="KB953_002815"
     CDS             complement(2696..>2948)
                     /locus_tag="KB953_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBB6867720.1"
                     /translation="TEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVPHSNG
                     VTEGLNNKIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
BASE COUNT          963 a          523 c          691 g          771 t
ORIGIN      
        1 aggattgtta aaatttaatc ttagaaaaaa ataactggaa caagagctat ttgtttttac
       61 agcaaagaat ggtatatttt tgtaggaggt ggcttacttg aaagaagtcg ttatgataga
      121 tgctgcaaga acgccgatag gaaagtatcg cggaagttta agtccattta ctgcagttga
      181 attaggcaca cttgtgacta aaggattatt agataaaaca aaactgaaaa aagataaaat
      241 cgatcaagta atatttggaa atgtcctgca agctggtaat ggtcaaaatg ttgccagaca
      301 gattgctttg aacagcggtc tccctgtaga tgtcccagca atgacgatca atgaagtttg
      361 cggatctggt atgaaagcag tgatcttagc acgccagctg atccaattag gtgaagcaga
      421 acttgtgata gccggcggaa cagagagtat gtctcaagca cctatgctga aacagtatca
      481 gtcagaaaca aatgaatatg gtgaaccaat ttccagtatg gtcaacgacg gattgactga
      541 cgcattttca aatgcacata tgggattaac cgcagagaat gttgcaacac aattttctgt
      601 gagcagagaa gagcaggatc gctatgcctt gtcgtcccag ttgaaagcag cacatgctgt
      661 cgaagccggt gtattttctg aggagatcat cccagtcaag atttctgatg aagacgtgtt
      721 atctgaggat gaagcagttc gtggaaatag tacattggaa aaactgggca cgttacgtac
      781 agtattctca gaagaaggaa ctgtaacagc aggaaatgct tccccgttga atgacggtgc
      841 ctctgtggtg atccttgcat ccaaagaata cgcagaaaat aataatctgc cttatttagc
      901 aaccatcaaa gaagtagcgg aggtcggtat tgacccaagt atcatgggaa tcgctccaat
      961 caaagccatt caaaaattga cagatagatc aggaatgaat ttatcaacaa tcgatctttt
     1021 tgagatcaat gaagcatttg cagcttcttc tatcgtagtt agccaagaac tgcagttaga
     1081 tgaagaaaag gtcaatatat atggaggagc aatcgcatta ggccatccaa ttggtgcaag
     1141 cggtgcacgt atcttgacta cattagctta cggattgctt cgtgaacaga agagatacgg
     1201 tattgcttca ttatgtattg gtggaggttt aggcttggca gtcttgttag aagcgaatat
     1261 ggagcaaaca cataaggatg tgcaaaaaaa aaagttttat cagcttacgc catctgaacg
     1321 gcgcagtcaa ttaatagaaa aaaatgtatt gacacaagaa actgcgttga tttttcagga
     1381 acaaacactt tctgaagagc tctccgatca catgatcgaa aaccaagtga gtgaagtgga
     1441 aatcccaatg gggatcgcgc agaattttca aatcaatggc aagaaaaaat ggattccgat
     1501 ggcaacagag gaaccatctg tcattgcagc tgcaagtaat ggtgcaaaaa tctgtggaaa
     1561 tatctgcgca gaaacacctc agcggctgat gcgagggcaa atcgttctat caggaaaatc
     1621 ggagtatcaa gcagtgatca atgcagttaa tcatcgaaaa gaagagctga ttctttgtgc
     1681 aaatgaaagt tatccttcaa tcgtgaaacg cgggggcggt gttcaagata tctctactcg
     1741 ggaattcatg gggagtttcc atgcatactt atctattgat tttttagtgg atgtaaaaga
     1801 tgcaatgggc gctaacatga tcaactcgat tcttgagagt gtggcaaaca agttacggga
     1861 gtggttccct gaagaagaga ttctattcag tattttaagt aattttgcta cagaatcatt
     1921 ggcttctgcc tgttgtgaaa tcccattcga acgcttaggg agaaacaaag aaatcggtga
     1981 acaaattgcc aaaaaaatcc aacaggcagg ggaatatgcg aagcttgacc cttaccgtgc
     2041 agcaacgcac aacaaaggga tcatgaatgg tatagaagct gtagtggcag ctacaggaaa
     2101 tgatacacgt gcggttagcg cttcgatcca cgcctacgcg gcaagaaatg gattatacca
     2161 aggattaaca gactggcaga tcaaagggga taaactagtt ggaaaactaa cggtgccttt
     2221 ggctgttgcc acagtaggcg gagcttccaa tatcctgcca aaagcaaaag catctttagc
     2281 tatgttagat attgattcag ccaaggaact agcacaagta attgctgcag tagggttagc
     2341 tcaaaacctg gctgctttgc gtgcattagt tacagaagga atccaaaaag gccacatggg
     2401 acttcaagct cgttcattag cgatctcaat tggggctatt ggtgaggaaa tcgagcaagt
     2461 tgcgaaaaaa ttacgtgaag cagaaaagat gaaccagcag acagctatac aaatattgga
     2521 aaaaatccgg gaaaaataaa aaagcaatat ctttagaaga ataaagcaag atttacagtt
     2581 attttattta ctgcagatct tgcttttttg gctcttcgtc aaattgtgtt ggcgaagaca
     2641 aatttgaata aaacttagtc cgatatgcgg cgcgttctgt gcgccgcata tttttttagt
     2701 tttcaaagat cagaccttgt tgtaaataga tccgtgcccg aaagttataa aagttgcgat
     2761 aaccaaacgc aattcgttta atcagcttga tcttgttatt cagcccctca gtgacgccgt
     2821 ttgagtgggg caccttaaag gcatttgaaa tacccgattg gtagcgatgg aagatcgtga
     2881 atttcttttt aaacgactga ggtaacgtag atgaaattgt tttaagggtt tcttgaaagc
     2941 cttccgta
//
LOCUS       DADVXU010000174         2935 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.181, whole genome shotgun sequence.
ACCESSION   DADVXU010000174 DADVXU010000000
VERSION     DADVXU010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.181"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            141..1619
                     /locus_tag="KB953_002816"
     CDS             141..1619
                     /locus_tag="KB953_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HBB6867721.1"
                     /translation="MKKLSQKRLAQIIIDSRKSLKMTQQQLAVATGINRALISRLESL
                     DFIPSIQQLESLAEVLNFEPTDLFINESNKSNRTKHQSKNIAVAGTGYVGLSIATLLA
                     QHNQVTAVDILPEKVKQINNKKSPIQDDYIEKYLAEKNLNLIATTDGAAAYREADFVV
                     IAAPTNYDSKQNYFDTSAVESVIKLVLEVNPKAIMVIKSTVPVGYTESIRKKYNTKNI
                     LFSPEFLRESKALYDNLYPSRIIVGTDQTDKTLREASHTFADLLQEGAIKKDIETLFM
                     GFTEAEAVKLFANTYLALRVSYFNELDTYAEMKGLNTQEIIHGVCLDPRIGTHYNNPS
                     FGYGGYCLPKDTKQLLTNYNDVPQNMISAIVDSNRTRKDFIADRVLELAGTYEANSSW
                     NAEKEKEVVIGVYRLTMKSNSDNFRQSSIQGVMKRIKAKGATIVIYEPTLENNTTFFG
                     SKVINNLDDFKNISHSIIANRYDSNLDDVKEKVYTRDLFQRD"
     gene            complement(1687..2778)
                     /locus_tag="KB953_002817"
     CDS             complement(1687..2778)
                     /locus_tag="KB953_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HBB6867722.1"
                     /translation="MLELNTSLSNRTILITGAAGFIGSNLVLELLRTQSSIKIIGIDN
                     MNDYYDVSIKNYRLSEIETEAKNHPGSTWNFIKGSIADKATIDFIFDNYKPSIVVNLA
                     AQAGVRYSITNPDMYIESNIIGFYNILEACRHSYDNGESGVEHLVYASSSSVYGTNKK
                     IPYSTEDKVDNQVSLYAATKKSNESMAHAYSKLYNIPSTGLRFFTVYGPAGRPDMAYF
                     GFTDKLRAGDNIQIFNYGNCKRDFTYIDDIVEGVKRVMQCAPEKIDGEDGLPIPPYHI
                     YNIGNNTPENLLDFVDILQQELIAAGVLPEDYDFESHTELVPMQPGDVPVTYADTSAL
                     ERDFEFKPSTSLRDGLRKFAQWYKSFYEV"
BASE COUNT         1041 a          518 c          460 g          916 t
ORIGIN      
        1 aaaaaataat tgaaaatact taaaatcaaa aaatatcttt ttaatcaata aaaaaattga
       61 cagttttatt aataataagc tattctgtta tagaagaaaa aattgtttgt tattattagc
      121 aacaaaaaaa ggagtgcatc atgaaaaagt tatctcaaaa acgtttagct caaatcatta
      181 ttgattctag aaaatctctg aaaatgacac aacaacaact tgcagtagct actggtatca
      241 accgtgcctt aattagtcga ttagaatctc tcgattttat cccgtcaata caacaattag
      301 aatcattagc agaagttcta aattttgaac caacagatct atttataaat gaatcaaata
      361 aatcaaacag aacaaaacat cagtcgaaga atattgctgt agccggtact ggttacgtag
      421 gtctttccat tgctactctt cttgcccagc acaatcaggt aacagcagta gacattcttc
      481 ctgaaaaagt aaaacaaatt aacaataaga aatctccaat acaagacgat tacattgaaa
      541 aatatctagc tgaaaaaaat cttaatttaa ttgcaacgac tgatggtgca gccgcctata
      601 gagaagccga ttttgttgtc attgctgctc caacaaatta tgatagtaaa caaaattatt
      661 ttgatacttc tgctgtagag tcagtaatca aattggtgtt agaagtcaat ccaaaggcaa
      721 taatggtcat caagtcgacg gtacccgtag gctataccga aagtattcgt aaaaaataca
      781 atactaaaaa tattctattc agcccagaat ttctcagaga atctaaggca ttatatgaca
      841 atctatatcc ttcgcgaatt attgtcggca cggaccaaac tgacaaaact ttacgagaag
      901 cctctcacac ttttgcagat ttactccaag aaggagcaat caaaaaagat atcgaaactc
      961 ttttcatggg attcacagaa gctgaagcag ttaaattatt cgctaatact taccttgcac
     1021 ttcgagtatc ttacttcaat gagctggata cttatgcaga aatgaaagga ctaaatacac
     1081 aagaaatcat tcacggggtt tgcttagatc caagaattgg cacacattat aataatccat
     1141 cttttggata tggaggctat tgtttaccga aagatacaaa acaacttctc actaactata
     1201 acgatgttcc tcagaatatg ataagtgcaa ttgtcgatag taatcgtaca agaaaagact
     1261 ttatagcaga tcgagtgtta gaacttgctg gcacgtatga ggctaacagt tcgtggaatg
     1321 cagaaaaaga aaaagaggta gtcataggtg tataccgtct tacaatgaag agtaattcag
     1381 acaacttccg tcagtcatct attcaaggag ttatgaagcg aattaaagca aagggggcaa
     1441 ctattgtaat ttatgaacca acacttgaaa ataacactac attttttggt tcaaaagtaa
     1501 taaataattt agatgatttt aaaaatatca gtcacagtat tattgctaat cgttacgata
     1561 gtaatcttga tgatgtaaaa gaaaaagtat ataccagaga tttatttcaa cgagattaaa
     1621 aaataacacc tcagatttgt agtgtctgag gtgtttctat aaacttttat aagtaaaaaa
     1681 atacaattat acttcataaa aagacttata ccactgcgca aatttacgaa gcccgtcacg
     1741 caaacttgta cttggtttaa attcaaaatc tcgttctaac gcactagtat ctgcataagt
     1801 aactggcaca tctcctggtt gcattggaac tagttcggta tgagattcaa agtcgtaatc
     1861 ttctggtagc acccctgctg caattagctc ttgttgtaga atatctacaa aatctagtag
     1921 gttttccggc gtattgttac caatattata aatatgatat ggtgggattg gtagcccatc
     1981 ttccccatca attttctctg gagcgcactg cataaccctc tttactcctt caacaatatc
     2041 atctatatag gtaaagtcac gcttgcaatt cccataattg aatatttgga tattatcacc
     2101 agcacgaagt ttatcagtaa aaccaaaata agccatatct ggacgcccag caggaccata
     2161 tactgtaaag aaacgtaacc ctgtagacgg aatattgtaa agtttagaat atgcatgtgc
     2221 cattgattca ttacttttct tagttgctgc atacaacgat acttgattgt ctactttatc
     2281 ctctgtagaa taaggaattt tcttattagt accataaacg gaagacgatg aggcatacac
     2341 taaatgttcc acaccagatt ctccattatc atatgaatga cgacaagctt caagaatatt
     2401 atagaatcct attatgttag attcgatata catatctgga ttagtaattg aataacggac
     2461 tcctgcctga gctgctaaat tcaccacgat acttggttta taattatcaa agatgaaatc
     2521 aattgttgct ttatcagcaa tagaaccttt gataaaattc caagtagatc ctggatggtt
     2581 ctttgcctcc gtttctatct ctgataatct atagttctta atagagacat catagtaatc
     2641 attcatattg tcaatgccaa tgatttttat tgatgactga gtacgtaata actcaagaac
     2701 caaatttgat ccaataaaac cggctgcgcc agttattaat attgttctat ttgataagga
     2761 cgtgtttaat tccagcattt tttagcactc actttctttt tttcttatga atacatattt
     2821 gttatatatt aaaaaatctt atttagactt atctattgtc acttttatca ttctaatctt
     2881 tgcacgtagt agaataatta tacagatagg aaattgctaa gcaacccact tcttt
//
LOCUS       DADVXU010000175         2929 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.182, whole genome shotgun sequence.
ACCESSION   DADVXU010000175 DADVXU010000000
VERSION     DADVXU010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.182"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..1087
                     /locus_tag="KB953_002818"
     CDS             <1..1087
                     /locus_tag="KB953_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBB6867723.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1305..2447)
                     /locus_tag="KB953_002819"
     CDS             complement(1305..2447)
                     /locus_tag="KB953_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBB6867724.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2452..2637)
                     /locus_tag="KB953_002820"
     CDS             complement(2452..2637)
                     /locus_tag="KB953_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HBB6867725.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
BASE COUNT          974 a          536 c          456 g          963 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaaccac cggtggcaaa ccggtggcaa atccatgtaa
     1141 acatggttta ttttttacac tgttttcaaa caaacccttg ctatttcttc ggttcgtcct
     1201 cgtccatttt cgataccgac agctagctta taacccaagg tatatcaaca taaaaaaata
     1261 aaaataggct atgacagcag cgtgacagca aaaaagatgg aaagttaaat atcaaacctc
     1321 gtaatttcat ttgcgagtgc ttgatttgtt tctggatata agtgtccata tttgtccaaa
     1381 gtagttttaa tagaggcatg acctagacgc ttagatattg catagatatt tttattatgt
     1441 tcaattaaaa aggcaacatg cgagtgtctt aatccatgca tatgaattgg ttttactcct
     1501 gcaatttcag ctagtttttt tattctgctt aaaatagttc ttggagatgg cggtaatcca
     1561 tcaaaagaaa aaaccaattc tatatttttc ccaattgatt gttgagtttt tttccatgtt
     1621 tttaaatctt caacggtttg tctgtctaac gaaattatcc gaatacttga tgtatttttt
     1681 gtttcagcaa attcataatc ttgtcttgta cggatgtaaa ttgacttatt tacgctacat
     1741 ctgccattta ttaagtcaaa gtctttccat tgtaacgcta agagctcctc actccttaca
     1801 cctgtaacaa aaataaaacg catcaaacgt ttataaaatt cttcataaaa atccccgtga
     1861 taaacactat tatatacttt cttaaattca tcaactgtcc aaaattctac ttgaggacgc
     1921 tgagttttta ttttacctat agtatctaca ggattttctt caataatttc aagtacaatt
     1981 gcccttttaa aaactactct caacttattg aatatctgtt taatataatt tggtgataat
     2041 ggttgctcaa tgcccttttt tacaacatgg attttcgata aaaactgttg aaattcctgt
     2101 atatgaattg gtcgaatctt tacaacctgc atttctccaa aaaaatccaa tgcacgttgt
     2161 aaaatatgat ctgttttcat atatgttttt tctacagttc ccaacttata ccaaggaaaa
     2221 aaatactctc gataaaaatc atgaaacgtt attccttgca gataaattcc ttgtgaatca
     2281 gaaaaacttt gttttaactc ttcgagtccc cttctagctt ctccttgagt tttgaagcct
     2341 cttctggtca cttgaattct ttttccaaag cgatcttgtc ctaaagttgc agaaaaaaac
     2401 caagtgcctc tttttctgtc tttataaaca ttcgcaattt tcctcatttt actattcctc
     2461 ccaagaaata tggagaactt cttctaaaat tgaaatagga attcttttta tttttgaatt
     2521 acgataaaaa aagtatcctt tctcaactaa aagttcttta cattccttga agattttatt
     2581 agcatagtac tgagaaaaac ctagattaat caaatctttt cgagtagctg ttttcattat
     2641 agcaccttcc ttactttttt tcatttattt ctatcagctg cttttgtcaa aagcacatag
     2701 gaatttcacc tctccccaag tttacggcga gccttacgga agtatcatta tcccaacttt
     2761 aaatcatcgc tttttattga ctataaaaat tattttagaa tatttatcgc tccttcttta
     2821 cagcaagaaa tcatggcgca actaaaatat gctccttaaa gtgttaacgt actgatagaa
     2881 tctatttaat tttcaatgaa caataggttt tctacctaaa agaatctac
//
LOCUS       DADVXU010000176         2839 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.183, whole genome shotgun sequence.
ACCESSION   DADVXU010000176 DADVXU010000000
VERSION     DADVXU010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.183"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            80..1768
                     /locus_tag="KB953_002821"
     CDS             80..1768
                     /locus_tag="KB953_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867726.1"
                     /translation="MKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLSRI
                     KGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLLNI
                     CTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYY
                     AYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFV
                     VVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDI
                     TTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLL
                     QFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVI
                     QSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKA
                     ADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIE
                     ITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            1755..>2839
                     /locus_tag="KB953_002822"
     CDS             1755..>2839
                     /locus_tag="KB953_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867727.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKN"
BASE COUNT          960 a          419 c          465 g          995 t
ORIGIN      
        1 aaatttaacg caaaacttat taaaaaaaga caattctagt atactgaaaa tagaattaaa
       61 aaaggagaac aacaaatcta tgaaaataag taaaaaagca tttcttttag tagttcttat
      121 cttacttagt actttatatt cagtaaattt tatgagaaat gctcaagaaa tttatacaac
      181 aggtgattta tcttttcatc tttcacgaat caaagggtta tcttctattt ttgaagggcc
      241 aatcaactat acaacattta acaattatgg agatggactg aactattttt atccttttct
      301 tacaataatt cctgcagtcg ttttttatgg aatttcaaat aacttaatat taagttatgt
      361 tctatatatt tggttattaa atatttgtac gattctgatt agtttttatt atggagaaaa
      421 gttttttaaa aagatagatg ctgctttttt atttagttgt ttatatactt ttgcaactta
      481 tcgaacagtt gatatctatt atcgatccgc aattgcagaa gcaatcgctc ttacgattat
      541 cccagctgtt ttatattatg cttatggatt gatattcaat agagaaaaaa aagtcatcgc
      601 tttatctgtc agtataagtc tacttgtgta tactcatgtc ttaactacac tattgtgtag
      661 tgcattgatt gcttgtatga ttatagttcg tttgttttta agcaagcaaa taaaaaacga
      721 tataaaattt gtggtcgtga atttagtaaa ggctgttttt ttgacttgtg cattaactag
      781 ctatttttgg tatcctatgt tgcagcagat gaattatcaa gaaatacaca gtcccttcaa
      841 aacagatttg cagctagaag cactttctat ttcagatagt atcattcaag cgttaaataa
      901 cgatattaca acatacacta tggggttatt aggattggtt gcgttgattt ttcctttatt
      961 catactgaaa gaaatggaga aaaaagaaaa aatcatctat gttggcgtag tttctacatg
     1021 ggtatgcgta actaatttat ttccatggtt tttgtttcaa aaaacaccga taagcttact
     1081 ccagtttcct tggcgtatct taggtatcca aatctttttt tcttcaatga tcataacgct
     1141 tgtttttatg aaaaagacta tgaataaaaa atattcatgg ttaatcattg gcgctactgt
     1201 ggtgtttcta ctcgtcacgt ctatagcagc aaaagaaaat tacgcaaaag ttatccaatc
     1261 aaaacacggt catattgtta tcaatgaaga aacactccca cgttatacaa catcagatat
     1321 gggaggatta tacgattacg ccccaacaga agcgataaaa gaaaggcctc acttagaaaa
     1381 acatgaagta aaagtaggag aatcgtggga aaaagggaag tataaagctg cagataataa
     1441 cattacttac acagtggcaa gcaacaaaaa gcaaaaagtg acagtaccag tatatgctta
     1501 tttaggtaca caggtaaaag taaacggtaa agtcgtaaat gactcttata aaaaacatgg
     1561 cttagtgaat gtggatgtaa atgctggaga aaataagata gaaattacaa caaaatatac
     1621 tccaactgct ctcatggctt tatggatcag tattctgact tattgtttag tggtgtattc
     1681 aagttttaaa cgatttgcac ctaataagtt gaaagattct cgatatgtga ctaaaaagga
     1741 gataaacaag aagaatgaaa ataaataaaa aagtagtaat attgagtgga ttaattatag
     1801 ttagtagtat atattctttc atctttttca aaaatacaca gagtatttat accactggag
     1861 atctttcttt tcacttatct cgaatcaagg ggttatcgac tatttttacc agtccgatta
     1921 attatgagac attcaattat actggatatg gtgtaaatta tttttatccc tttctgactt
     1981 tttttccagc tgtaatgtta tattggatga caaaaaattt gattgtaagc tatattattt
     2041 acgtttggtt attgaacttg tgtacaacac tcgtagctta tcattatggc gagcggtttt
     2101 taaaacaaaa aaaagcggca tttttattta gttgtctcta tattttctct gcttatcgga
     2161 cagtagacat ctactatcgt tccgcaattg ccgaagctat cgcaataaca cttgttatcc
     2221 ctgtattatt ttatgcttat caaatcatct ctggaaaaga agaaaagtat ccgtctgtaa
     2281 agctagcatt gagtatgagt ttgctagtgt acagccatgt actatcaaca ttaatgagta
     2341 cagcattaat tatcattttt atttttattc gtttggtttc taaaggtttc aagaatgcag
     2401 attttattgc tatatttaaa aaattattta gtgcggcagg aatgacattg gttctgacga
     2461 gtgctttttg gtatccgatg tttgaacaaa tgctctacca aaaaatcaac aaacctagcg
     2521 tgacaaattt atatgctcat gcatctaatg tgttcgatag tctgactgaa gctatgaaca
     2581 acgacttaac tacttactca atgggactag ttggtctgtt atctttatgt attcccctga
     2641 ttttatttaa aaaattaaca aacatagaaa aaaagattta ttatggtacg tgtttgactt
     2701 ggctagctac aactagtttg gtgccatggt atcttctgca aaatacgcca gccaaattgt
     2761 tacaatttcc ttggcggatc ttaagcttgc aaataatatt ctcttctctg atattaacga
     2821 tgatattttt caaaaatag
//
LOCUS       DADVXU010000177         2769 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.185, whole genome shotgun sequence.
ACCESSION   DADVXU010000177 DADVXU010000000
VERSION     DADVXU010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.185"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(76..1875)
                     /locus_tag="KB953_002823"
     CDS             complement(76..1875)
                     /locus_tag="KB953_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBB6867728.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(1999..2595)
                     /locus_tag="KB953_002824"
     CDS             complement(1999..2595)
                     /locus_tag="KB953_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBB6867729.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT          977 a          522 c          480 g          790 t
ORIGIN      
        1 aaaaacgcat atacctgcgg tggcaggtta tgcgtttttt gttgtataaa gacttttttc
       61 attaacgatt tttgattagc cgactcccaa aacaaaattt agcagtttat ctgaataagt
      121 gatttcttca tctgtttgtt tcacactttc atttagtagt cccatctcat cggttatgat
      181 accatccaca ccataaaaca tcatgcgact catagcatct gaatcgttga ttgtccatac
      241 atatacttct tttccgtctt ggtgggcaga attaatgaag ttacggttga tcgtactata
      301 ttccattgtc agaaaatcag caggcgtcac agggggccca acaatattga aaggtaagat
      361 gtagcccaca taaaagtttg gttctgcttc ttttaaatct gaaacggttt ggtacgtcaa
      421 actttgcaaa atatgatggt tctctaagat aactttgcgg tatttatgga caaatcgttc
      481 gacaaggtca tccgaatctt ttttagaggt cttgatctca ataagtaatt tttggtttag
      541 cttatgtgct ttttccaaat aagaatcaaa agaacaaaca ggggcttgca ctccgttttc
      601 agtaactgta agatttgtca attctttcaa tgtaagatcc tgtggtgttc catttacccc
      661 tgttaagtga cgaagatttg catcatgcat cactacgaaa tgaccatctt ttgtttcctg
      721 aacatccatc tctacataat caggatgata gtctctgctt gttttttcaa gtgcttctaa
      781 agtattttgc gccccatttt gggtactcac acctctgtga gagacagtca ctggttttgt
      841 ctgaacagct gaagtcagat aatccatatt gtaaagacag acgccaatac cgaaaaaaac
      901 tgcaaaaaga gtcaaaccag tgtttttcag cgcagagaaa cgcaagttgg gggcttgtgg
      961 gataaaccac ttagggatct caggcaagaa gccttcgtca tccatgaaat caacaatgat
     1021 ataaaaaatc cctacggtag acatcacgat attgaataat aaaatccctt gtaaaagcgt
     1081 catagcaaac acagcgctga tcaatgcata gtctgggaaa aattgttcaa ccatactttg
     1141 tgccaaaata acaacgatat atcctaaaga agaaaggagt aaaatcgtac cgccaatcac
     1201 aataaattgg cccgtgatgg ccaaaagacg cgatttggtc aatgaccagc tttcccgaat
     1261 ggctgctcga aaaggacgat ctcgtagaat catttccggt aaagcaaaaa tcagtcgaat
     1321 cccaatatag ccaaggaaaa tataaacgag taaaaaggaa ctgacgatga tccaacgatt
     1381 agcaaaaata aaatccataa tgaaagcggg gatttttatt tttgacagta gatcagaatt
     1441 aaaacttaat ccgctgatcg gtagaatcaa taaaaaataa gcaaggaaaa acaaaaaagt
     1501 aatgggtctc actttcttca gctgaagtat cgtcaagtgt aagagttgct tcaaagagat
     1561 tggttctttc tttttaataa aaaaaacact catcaaaaga aaagtaaatt cgaaataaac
     1621 taacagtaaa atcgtcatca aaactacgat taacgagagt agaaccccag gatgttgaga
     1681 aaaaattgaa ggaatattgt cataagacaa atagtcaatt gcccctcggt gtaaaataaa
     1741 acgggtcgta ctgctcaata agggtaaaca aataaataaa ataaagccat gcatcagcaa
     1801 gacatcacgg aaataagctt gtgttccttt taaaaagtcc caagcattac ctaagctacg
     1861 tttcaaataa ttcatcctat cacctgctct tcacattgta gcgcaaatct aatgaaaaag
     1921 gaaagggtgc agtgtgtttt gcagaccctt tcccagattt aacattgtca atcaatgact
     1981 aattttacaa aataaatatt atccaagaag accacttaga tattgacctc cataaatcaa
     2041 ggccatacta tataagaaaa tcgacagtgg tttagcaatc aaaatgatta gtgtatatcg
     2101 tttcactgac atgtttgtca atccagccat caaacataac gcatcgtcag gagcaactgg
     2161 aagaaaaata gctagagcaa acagcttctc aaaacggttt tgattatcca gccagccaat
     2221 atatttgtta tatgtcttat cgctaatcat ggaaagtatg aatggctttc cataacgacg
     2281 ccctagcaga aaaatgataa tcgaaccaat gcagattcca atatagttat agataaatcc
     2341 agcataaggg ccaaatatca ggacaccagc tgcagtactg atccctccag gaatgattgg
     2401 aataactact tgcaggattt ggatgaagac aaaaataatc ggtcctaaaa tgatggaatc
     2461 acctactaat cctcttaacg cattcaaatc tttgaaaaca cctaaattaa taaaataaat
     2521 cgtcagcgcg attgataggc ctaatccgat aatggatatg aaatttatca gttttcgaga
     2581 ggcagcaata ttcattattc atcaactcct tagaggttct ttgttaacta tactttacta
     2641 gaaaagcaac taactgcgcc attttttgat atgacagaaa acaaacaatt ttgtaagttc
     2701 atcataagta taaaagattg atctcaaaaa aacatccaac taaagactta tttaataact
     2761 tgttttttt
//
LOCUS       DADVXU010000178         2768 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.186, whole genome shotgun sequence.
ACCESSION   DADVXU010000178 DADVXU010000000
VERSION     DADVXU010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.186"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            204..1529
                     /locus_tag="KB953_002825"
     CDS             204..1529
                     /locus_tag="KB953_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBB6867730.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            1522..1872
                     /locus_tag="KB953_002826"
     CDS             1522..1872
                     /locus_tag="KB953_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867731.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            1869..2039
                     /locus_tag="KB953_002827"
     CDS             1869..2039
                     /locus_tag="KB953_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867732.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHKYEYYIGY"
BASE COUNT          975 a          438 c          536 g          819 t
ORIGIN      
        1 ataatacgac acttattttc aaattttcac aagactaata gtaaatatcc atttaataaa
       61 gtgatacaat gtgttgattt tacaaatcaa cagaagaagt agggaaataa cttacctatg
      121 cttctttttt tgtacattta atcgattctt ctttacaaaa acgaacgtat gtttgtataa
      181 tgtggaagaa tggagtgtta tttatgatgg attattcaaa agagccagta aatgattact
      241 ttttgatcga tatgaaaagc ttttacgcaa gtgtcgaatg tatagaaagg aatttagatc
      301 cattaacgac tgaattagtc gtcatgagtc gagcggataa tacaggatca gggttgattt
      361 tagcttcttc accagctgca aagaagaagt acggtatttc taatgtcagt cgaccacgag
      421 atctaccgca accttttcct aaaacgttac atgtcgttcc accacgcatg aatctctata
      481 ttaagcgaaa tatgcaggta aacaatattt tcagaaggta tgtagcagac gaagatttat
      541 tgatttactc gatcgatgaa tcgatcttaa aagtgaccag atcaatgaat ctttttacga
      601 ctgaaggaac aagaagtcaa cggagaaaga agctggctca aatgatccag gagaatatta
      661 aagaagagct aggattgctt gctacagtag gtgtcggaga taatccctta ttagctaaac
      721 ttgctttaga caatgaagca aaacataatg atggattcct tgctgaatgg acgtatcaaa
      781 atgttcctga taaagtatgg aatattcctg aaatgacaga cctttggggt attggatctc
      841 gaatgaaaaa aagactcaac caaattggta tcatgagtat taaagactta gctaattgga
      901 atccatacac tattaagagc catttaggtg tgattggctt acagctgtat tttcatgcga
      961 acgggatcga tcgaacagat atagcgatcc caccagaacc gacaaaggaa aaatcatatg
     1021 gtaattcaca agtgttacca cgagattata ccaggagaaa tgaaatcgaa ctggtggtaa
     1081 aagaaatggc ggaacaagta gctattcgta tcaggcaaca taactgtaaa acaggttgtg
     1141 tccatttata cattggcacg tcgatcttag aaacgagacc aggtttttca caccagatga
     1201 agatccctat aacagataat acaaaggaat tgcagaatta ctgtcttttc ttgtttgata
     1261 agtattatga aggacaagaa gtaaggcacg ttggaataac ctatagcaag cttgtgtaca
     1321 ctgattcgct tcagctggat ttatttagtg atccacaaaa gcaaattgac gaagaaaatt
     1381 tagataaaat tattgacaag attcggcaaa aatatggttt tacgtcgatt gtacatgctt
     1441 cttctatgtt agaaggcgct cgtagtatta ctagaagtac attggttggt ggccatgcag
     1501 gcggcgcagg agggcttaaa aatgattgat tataatgatc gtggacgttt aaagtgggca
     1561 ggtttttatt tatctgatca tactgaaaaa attgatgccg acaatgaaca acgagccaat
     1621 caaaatttgg caaaagaaca aatgacgact gaagaggttg cggaagtatt gaacgctgca
     1681 gtcataaaaa atagatcagt atgtattcaa aaagaagaac gaaacgctga aggatactat
     1741 ccagaagata ttgttagcaa gatcttaggt agtgatgagc tggggatcta cgttggaaaa
     1801 aacaaagttc actacgacga aatacgtcat atcgaatttt accaggataa taaatggggt
     1861 cgattagaat gaacattatc cagcaatatg agctaaagta tataaccttt gatcagctat
     1921 cagaagagat ttggggttat ggccagcgtt tgataaatga ggttggtgtt gaacgcttct
     1981 ctttctatgt agaagctgca gcaggatacc ataaatacga atactatatt ggctattaaa
     2041 gtgcaataaa attatatttc ttttatgttt tacattttgt atcaattctg ataaaattac
     2101 ctataggtaa ttaaaaaata taaaaaaact agttacaacc ttaggacgtt gtaactagct
     2161 tgaagcatac tagagctaac taaaatagca ctagaaagca atgttaaacc cgtccaacaa
     2221 acttgattta acattgagac cagcttattc agctaaatat agttgaataa gcttcttttg
     2281 ttttaaaaaa acaaaaggtg tactacgaat aacgcaagta aaagaaatac tattacaacc
     2341 agtaggctac aagtaagtat tactaaacat tgcgtagtta gaacctttgc tctattgtct
     2401 aaagccacaa aattagagat cagctttatg atgctaagag cgagctgcat ccatgcggac
     2461 ataggttctt ttgacactat taattctcct ttttaggata agggaaattg ctagtagttt
     2521 tttcattagc accatccccc ttcatttgat tgagtctagt gctgctgtga tagtttgctg
     2581 tttaatatgc ttacattctt attataacat aaatgaaaca aacacatggt tagaggtcgt
     2641 atcaatcaaa atagtgtctc acaaacgatt tattacttac tcctgctgac taacggactg
     2701 ttcttcagta actgtaacta tatgtttgtt aaaaccacca ttacctgtag ataaaaacca
     2761 atatattt
//
LOCUS       DADVXU010000179         2675 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.187, whole genome shotgun sequence.
ACCESSION   DADVXU010000179 DADVXU010000000
VERSION     DADVXU010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.187"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            690..785
                     /locus_tag="KB953_002828"
     CDS             690..785
                     /locus_tag="KB953_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HBB6867733.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(1087..1332)
                     /locus_tag="KB953_002829"
     CDS             complement(1087..1332)
                     /locus_tag="KB953_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867734.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            1677..1961
                     /locus_tag="KB953_002830"
     CDS             1677..1961
                     /locus_tag="KB953_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867735.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            1964..2380
                     /locus_tag="KB953_002831"
     CDS             1964..2380
                     /locus_tag="KB953_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867736.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
BASE COUNT          882 a          497 c          430 g          866 t
ORIGIN      
        1 cgttttttca tccattcctt tcttcccccc ttacaaataa ataataacct ttaacaacca
       61 actcgtaaat aggttggttg ttattataca aaaggatatt agtactcaga taaaaagttg
      121 ttagtactca gataaaaagt tgttagtact cagataaaaa gttgttagta ctcagataaa
      181 aagttgttag tactcagata aaaagttgtt agtacccttc tgtatccgtt gggagagtaa
      241 ggctcaactg ccccctaaaa agggttctaa aaaatcttta aaaaataaat aaaataaacg
      301 cgcgcgcgcg agcaaaattt cccgccaaaa aatcacccac tttttaaaat aaacaggttc
      361 gcgcatgata ttaaactgcc aatctagctc cctatggtcg ctgtaagaag gagtgatcgc
      421 tacgctctca cattttactt gcagcagtcg cctttaggct ccttcgccaa aaataattca
      481 ttagggactc ctttgtggcg tcctgagagc tcgtctaagc cgttctaaga caagattcga
      541 taaaaactat gtccttcgtt ctaaagcgcc ttacaggcgg tcagggcaag ccctaacaac
      601 cccaaggcaa taaaaaactt aggcttattt ttgctttttt attgtataat tgattgtcgc
      661 ctatccgaca gtcttgcgac tggaaggagg tgtgtttcgt gttcactaac tttatttgct
      721 cccttcttgt tgggctttta agcgctttgt tcagttactg gttgaataac cgaaacaaga
      781 agtaaaactt aattaaggcg actcagccca caaaaaaagc cccagagaat ccgacctctc
      841 tggggctttc gtgtgtttcg tgttcactta tttgcaatca aattataaca gaaataaata
      901 tttatttcaa aaatatccta caaaagtcta aaatccgtaa ttatttctta tatgcaacat
      961 ttacaaataa agtcgctctg agagctcgta tttttcgatt taagacgttt tatttagttt
     1021 ttggtgtatt tcattggtcc aagctaaaaa actcttagaa gcgattttag agccatttaa
     1081 acccacttaa ttatattctc catgaaattc gctccatgcg tcaaaagctg cgtctgaaat
     1141 tccgccgtct tcgttcacta gttgcattaa tttttccaac gatcgttttc tttcgggtgt
     1201 catattttta tattccaaca atttttcgtc tgttaaatcg aacttgattg cttgaattgc
     1261 ttgctctaat ttttctaagt tttgaaaata ttcgtaagga aatttttcca ttttttcttt
     1321 ctccagtctc acaatttttc tagaaataaa atcacttctt tgccgtctat actctcaaaa
     1381 aaaatgtcgt ttgaaaaact aaataaattt ttgatcgtgc atttttaaat ttggttatcg
     1441 attacacgtg cagcactccc ccccgaattt taaagagcat aaggaaacgt gtacccactt
     1501 tctaaaaaaa gtgggtagat aggctgtgcc tatcacgttt ctcagaaaaa aatttgatat
     1561 cactttttcg ccatcgtggg tagcttatgt atagcatttt ggcaaaaaac agctatacaa
     1621 atatagtatt tgtatggtat acataaatta tacataattg caaaaggaga ttttttatgg
     1681 acattttagt tcggaatatt gatccaaagt ttgttgcaac aatagacaaa cgttgccaag
     1741 aactcacgaa taaaacagga gaaaaatgga gtcgaaataa atatctaaaa gttttaatcg
     1801 aaagtgattt tgaccgtcaa cttacgactt acaaaaaaga ccaattcgat ttattgctcg
     1861 ataaatttat tgctatccaa acagaaaata ctcaaatatt aagcgagtat gtctacacaa
     1921 ataatcaaat cattgaaatg cttatcggag gaatgatata accatgagaa aaacttctgt
     1981 ccaaaaacat gttactttgt accctagtca tgttgacgtt ttagattcca tcatcgaaaa
     2041 aaatccagat atcaataact actcggaagc cattcgcttt ttatgtatga gttatgaacc
     2101 cgataccaac acgaccacta ccagcaatca ggcaaaacta aatgctatgg gaaaagaaat
     2161 ttccatcatg actgaaatca tcgcagaatt tgctgattta tatctaaaag atacaggcgt
     2221 tcttattggc acaaattcta aagtatatca agaagtgaaa gaaaaaatcg aagctaaact
     2281 aaatccatca aatcgaaatc gaccaaagaa tcatcaaaaa gaaaccagtc aaacagtcac
     2341 ttctagaaat ttcgacccaa caaattttct aaaacgataa aaaacagata ggagttattc
     2401 ctatctgttt tttatttaga agccccatac aatatgctag aatgtactag caatcttagt
     2461 cggttgtaag ggacttgtct acttgcttaa tgtaaggggg gcgagttctt ttttatttat
     2521 tcttctaacc agttacgcag agaaatcttt ggcaaatctg acgttaggct tgaaaaagta
     2581 aattctaatt tggcgatttt attgcccttt tttgctttta tttttgtgac tttaagatca
     2641 ttgaagtatt tttttagttc tcgtaatgca ggatt
//
LOCUS       DADVXU010000180         2630 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.188, whole genome shotgun sequence.
ACCESSION   DADVXU010000180 DADVXU010000000
VERSION     DADVXU010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.188"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..574
                     /locus_tag="KB953_002832"
     CDS             <1..574
                     /locus_tag="KB953_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HBB6867737.1"
                     /translation="KYHLKFEKKKYVLQVFHPVVTELENIEKQILALINSMEQQSQPI
                     ICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTILKECKFMIGNSSSGIIEA
                     ASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKIQDPQFIKSLKNVKNIYGD
                     GNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            547..1224
                     /locus_tag="KB953_002833"
     CDS             547..1224
                     /locus_tag="KB953_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBB6867738.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            1214..2356
                     /locus_tag="KB953_002834"
     CDS             1214..2356
                     /locus_tag="KB953_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBB6867739.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            2369..>2630
                     /locus_tag="KB953_002835"
     CDS             2369..>2630
                     /locus_tag="KB953_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBB6867740.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCN"
BASE COUNT          986 a          343 c          510 g          791 t
ORIGIN      
        1 aaaatatcat ctaaaatttg aaaagaaaaa atacgttttg caagtatttc atccagttgt
       61 aaccgaacta gaaaatatag aaaaacaaat tttagctttg atcaattcaa tggaacaaca
      121 atctcaacca attatttgca ttttacctaa tagtgatgct ggtagcgaaa ttatcagaaa
      181 aatgtaccag aaatatttaa aaacggatat tatttatatt acgaatttag agccggagga
      241 atacctgact atattaaaag agtgcaaatt tatgatcgga aattcttctt ctgggataat
      301 tgaagctgca tcattcaaaa taccagtaat aaatttagga accagacaaa atggacgaga
      361 acaatctaaa aatattataa atgcaaatct aaatgaaaat gaaattgaaa ctgctttgaa
      421 taaaatacaa gatcctcagt ttataaaaag cttgaaaaat gttaagaaca tctacggtga
      481 cggcaacagt tctagaagaa taacaaaaac attgaatcaa atattgaatg atggggaaga
      541 tattaagtgg attcaaaaac aaatagctta ttaaaatata ctagtgatcc atacattatt
      601 attggggctg gcggacacgc aaaagtcatt attgaaacct taagattaga aaataagtta
      661 atatatggat tttttgatga taagcaaaat aaaatgcaag atctgaaata tcttgggaaa
      721 ataaacgaat tgaatagctg ggcggatagc aaaaaaaaaa tctataaatt tattgtagct
      781 attggtgact gtgtagtaag aaaaaagatt ataaaaaaat tgaatatgcc aagtgagcaa
      841 tatggaatag ctgttcatcc aagtgctata gtggcaaaag aaatttcaat tggttatgga
      901 actgttattt ttgcgaatgc tgtaattaac ccagatgctg taattggaga gcatgctata
      961 ataaacacag gtagcattgt tgagcatgat tgtagaatta ataactatgt tcatttatct
     1021 ccaggtgtat gcttagcggg tggagtacat gtaggtgtag gaactcaaat aggaattggt
     1081 tctcaatgta tccagatgaa gaaaattgga tcttggtgta tgataggcgc agggagcact
     1141 attgttaagg atattccctc acattcctta gcttatggca atccagctaa aattaagaaa
     1201 gaaggaatta attttgaatt ctagaatttt attatcaagt cctcatatgg gaggaacaga
     1261 agaaaaatat gttgctaagg cttttaaaga taattggata gcgccattag gtgaaaatgt
     1321 ggataaattt gaagaaagta ttacaaaata tactggttct aagggcggag cttctgcgtt
     1381 aagttcaggt acagctgcca tacatcttgc cttaagactg cttaatgttg gaaaaaacga
     1441 tattgtcttt gctccttcat ttacttttat agctacagta aatcctattt tatatcaagg
     1501 ggctgtacca gtgttcattg actcagaacc taatacatgg aatatgagcc ctgttgcact
     1561 agagagcgca ttagaagaag ctgaaagaaa caaccaatta cctaaggcaa tcatcgtagt
     1621 gaatttatat ggtcaaatgg ctgattatga acgaatttta gctattgcaa accaatttaa
     1681 tgttccagtg atagaagatg cagcggaatc gttaggttct acttataagg gaatttcgtc
     1741 aggcttattt gggacaattg ggatctattc atttaacggt aataagatca tcacaacctc
     1801 aggtggagga gctctagtgt ctagtgataa aaaatatgtt gagcattcaa gatttttagc
     1861 aacacaagca agagatcaag caccgtatta tcaacatagc gaaataggtt ataattaccg
     1921 tctaagcaat atttctgccg gtatcggacg tggacaaatg gaagtattga atgatagagt
     1981 agctaaacga agagaaattt tcaatcgata taaggaagct ttttctggaa ttgatagaat
     2041 tgaaatgatg ccagaagtgg atggcatggt gccaaatcgt tggttgtcta ctttaacgat
     2101 caatgattat aattctattg atcaagtagt taacgtaata aataaactaa atgaaaaaaa
     2161 cattgaaaca agagctttgt ggaaaccact acatttgcaa ccagtatttc aaggggtaaa
     2221 aatgtattac catgataata atcttagtgt gtcagaaaag ttatttaata gcggcttatg
     2281 cttaccttct ggaagtaata tgacagatag tgaacaagaa tttgttattg atgagtttaa
     2341 aaaaatattc aagtaggtgt gatattttat gaacatagga attattggtt taggttccat
     2401 ggggaaaaga agacttagac tcattcaaga acactttaat gatattttac tatgtggtgt
     2461 tgatactaat gaggcgagac gaaaggaagt tgaaaaggaa tttggaatac caacttataa
     2521 tagtctaaca gaggcaaaaa atttgtttga aataaattca gtttttgttt gtacatcccc
     2581 tataacccat gaatttatta taaaagaggc tttaaattta gattgtaata
//
LOCUS       DADVXU010000181         2629 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.189, whole genome shotgun sequence.
ACCESSION   DADVXU010000181 DADVXU010000000
VERSION     DADVXU010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.189"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..285
                     /locus_tag="KB953_002836"
     CDS             <1..285
                     /locus_tag="KB953_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HBB6867741.1"
                     /translation="LSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNI
                     YIENELTDESQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            412..1047
                     /locus_tag="KB953_002837"
     CDS             412..1047
                     /locus_tag="KB953_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HBB6867742.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWVLIVLLFFGIGGALSGGE
                     NKDSATATSTSTNKETTKETTTKEEKTYKIGDDVAVGKMEYKVNSVEVVKQVGPSVLP
                     TNAKDTFLVVDLSVKNAGDKAVTVDSSFFKLKADGKTFEADSAASMSANQDENGNITN
                     SFFMESLNPDMQQTGKIVFDISEAQANAKNNVLQAQTGYFGTETVSIALHN"
     gene            1166..2377
                     /locus_tag="KB953_002838"
     CDS             1166..2377
                     /locus_tag="KB953_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBB6867743.1"
                     /translation="MSEVTGYLEQVDNETYRLRAVLGYKPDGSAKRKSKTIKASSKRA
                     AYKELNIWLEQFEGMTDDSLDLFNITFGEFYRKIWLSEAEKNLEPKSYHNYKRMIENR
                     FLDKFDFIPLIDIKPYMIKKIVVNAQRINTKDPGRNSDKPLSRNTKLRMLYAVNNLFL
                     MAKNEYGAIKENPVENVKIPKEKGVKKNIEEPYSEEEIHAMLKAAFEESIEIKTLIVL
                     AFITGARQGEIAALEEKDIDFDKQEIRFHQRISEVDGKSDIRLLPGLKNDDDEKIVTG
                     PAYLFDMLDELIKENKKIRWKLNIKKLKHYFIFDTKQDGTLPRGSYLYKKFKRFTKRH
                     NLRHIRFHDIRHTSATYLLSDPNMTPKELQKRLGHRDFNTTMNVYGHVLRKEKDTATT
                     AFENLLKKDIK"
BASE COUNT         1016 a          410 c          466 g          737 t
ORIGIN      
        1 ttatctcatg aacttgcaca tttcagcgcc tctcattttg aattttcagt tttgtacgat
       61 acttctacaa cttttcactc aaaatttgaa acagaagcag ataaaatagc tattctaatt
      121 ttattaaata tctatattga gaacgaactg actgatgaat ctcagtttaa cctcgaaaaa
      181 tttatggaat attattctat tcagaataag ctcagataca cttgttatgc tgtctgccaa
      241 tgctatttta agaaaaaata ttcttacgca aggcaatatg tatgaataca tcaagaatta
      301 ttaaaaaaac cgatttcagc aaaacattaa aaaagcccgt gctggcacac ggactcatac
      361 ctcatttctg agatcgcaaa tatattataa caagaagtga ggaatattta aatggcaaaa
      421 aaagttatgg gtcaagatgg gaaaatgtat aaggttaaaa aaccgtttta taaacgggta
      481 tggttttggg tattaatagt acttttgttt tttggtattg gtggtgctct aagtggcggt
      541 gaaaacaaag attcagctac tgcaacatca acaagtacta ataaagaaac tacaaaagaa
      601 actacaacaa aagaagaaaa gacttataaa atcggtgatg acgttgctgt agggaaaatg
      661 gaatacaaag taaactccgt tgaagtagta aaacaagttg gcccatctgt acttcctact
      721 aacgcaaaag acactttctt agtagttgac ttatctgtaa aaaatgcagg tgataaggct
      781 gtgactgttg atagctcatt cttcaaatta aaagcagatg gaaaaacttt cgaagctgat
      841 tcggctgcat ctatgtcggc aaatcaagat gaaaatggta atatcactaa ctctttcttt
      901 atggaaagct taaatcctga tatgcaacaa actggtaaaa tagtttttga tatctctgaa
      961 gcccaagcaa atgcaaaaaa taatgtattg caagcacaaa caggttactt tggaacagag
     1021 acagtttcaa ttgctcttca caactaatta gtataaaaaa cacgccccac cgaccaaagc
     1081 gagcgtgttc taagaaagaa acaaacctat acgataggct tctttatagt acctattgta
     1141 tcagagaaag agagctgatt caattatgtc agaagtaaca ggatacttag aacaagtaga
     1201 caacgaaaca tatagactaa gagctgtatt aggatacaaa cctgatggct cggcaaaaag
     1261 aaaaagcaaa actataaaag caagtagtaa aagagcggca tataaagaat tgaacatctg
     1321 gttagagcaa ttcgaaggaa tgaccgatga ttcgttggat ttgttcaata taacttttgg
     1381 tgaattttat agaaaaatat ggctttcaga ggccgaaaaa aatttagagc caaagtctta
     1441 tcataactat aagcgaatga tcgagaatag atttcttgat aaatttgatt ttatccctct
     1501 aatcgatatc aaaccataca tgataaagaa aattgttgtt aatgctcaaa gaatcaatac
     1561 aaaagatcct gggagaaact ctgataaacc tttatcaaga aatacaaagc ttcgtatgtt
     1621 atacgccgtt aacaacttat ttttgatggc taaaaatgag tacggagcaa ttaaagaaaa
     1681 tcctgtggaa aatgtaaaaa taccaaaaga aaaaggcgta aaaaagaata tcgaagaacc
     1741 ttattcggaa gaagaaattc atgcaatgct aaaagcagca tttgaggaaa gcatagaaat
     1801 taaaacttta attgttctag catttattac aggagcaaga caaggagaaa ttgcagcttt
     1861 agaagaaaaa gatattgact ttgacaaaca agaaataaga tttcatcagc ggatttcaga
     1921 agtggatgga aaatcagata ttcggttgct gccaggtttg aaaaatgatg atgatgaaaa
     1981 aatagtaacc ggtcctgctt atctatttga tatgttagat gaattaatca aagagaataa
     2041 aaagattcgt tggaaattaa atataaagaa attaaaacac tattttatat ttgatacaaa
     2101 acaagatgga actttgccac gtggaagtta tctgtataag aagttcaaac gattcaccaa
     2161 acgtcacaat ttgcgtcaca ttcgttttca cgacattaga catacatcag ctacctattt
     2221 gcttagcgat cctaatatga ctccaaaaga gcttcaaaaa cgattgggac accgagattt
     2281 taatactaca atgaatgttt atggccatgt cttacgcaaa gaaaaagata cagcaactac
     2341 agcgtttgaa aacctattaa aaaaagatat aaaataacac taaaaaatta tattttcggg
     2401 tataaactcg ggtaggatat acaaaaactt gggataattt gcggtatata agcatttaaa
     2461 aatgctgtaa attcgcttat cttcatttaa aataccatta aataacgaac tcttaatcag
     2521 cgggtcgcgg gttcgagccc ctcacggccc attgggtgcc aaacccacgg aacaggtaaa
     2581 tgttggtgcg aaagcatttc gaaaacattt acgctgttct ttttttatt
//
LOCUS       DADVXU010000182         2610 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.190, whole genome shotgun sequence.
ACCESSION   DADVXU010000182 DADVXU010000000
VERSION     DADVXU010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.190"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(865..1383)
                     /locus_tag="KB953_002839"
     CDS             complement(865..1383)
                     /locus_tag="KB953_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HBB6867744.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            2501..>2610
                     /locus_tag="KB953_002840"
     CDS             2501..>2610
                     /locus_tag="KB953_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBB6867745.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELT"
BASE COUNT          863 a          476 c          387 g          884 t
ORIGIN      
        1 atacacctac aacttataaa ttgatggtat ttagataaca cgaaagaata aaaataacag
       61 ctgatagtaa agtttgttga cgattgctac tcaatcgaaa gtgagttagt gcgtatgaac
      121 ttgcttctga aaacttatga aatgaaagac cttttacatc ttgcagaaat cgttactggg
      181 attaaactaa gcaataaaaa ctaatgttcc cctcattctt tgaacttcta ctatttgatt
      241 tatttattct gataaatgac gatttcacct tcaggatctt caaatcccgt atagaattta
      301 tttaagatct cttcagtatc agtaatagga taggcgatag gccacatacc ctttgatggt
      361 tagaataaac tgctcaaaat ctgtatcgta tgttggcttt agcatattaa caaaggctat
      421 ctccaaatta taaaccataa gcgttgaatc gttcttaaca atttcaattc ttcctaaccc
      481 aaagtcagaa gatataatta ccggacttgc cttacccttc atgtgggttg ttgagattcc
      541 atatttaaag ggaattgtcg gtactatcgg cggtctgaaa aacatcccaa aaataaaaaa
      601 atctttcccc tgctagtctc ctaagtccct ttgtagcgga tatggcacca taatctcatt
      661 ttatcgaagt gagtcatggt ttatctgttg gccaaccaaa attgatacaa tctaacgatg
      721 caccctctac ggcattctag gggagtgcac ttgcattcac ccaataaaaa ccggcagtta
      781 gcaaagaact tatgtagtcg tatgttctta ctgttaacat acaagaggtc tcctttccac
      841 aattgcttag cagtaaacgg gaatttacaa agctaatctc ttccattttc ttatctttgt
      901 tctaaaagtc ccaaatggag caactgtatt tacatgaata aatttatata cttcccatac
      961 agccgttttg gttgcctcgt ctgcccattt tcttttgtgt gcagtgaata gatcttcatc
     1021 actcattgaa tcaatcatca tgttaatatc atcaatatta cgattgagaa tttcctctaa
     1081 ttctttcaat ggtagatgtg cataggtatt tgtaaaccac ttatataact ctcctaactg
     1141 attccattta aattgatcag aaggcgtttt aacagataat ccatttcttt catcattttc
     1201 ccattttaga attaaattcg tccaaccaac ttgatatgct aaattttcag caggtgttct
     1261 atccacttcc tcaactcttt tatccttcaa ttcttcagga atagtttcaa attctaaaat
     1321 atatttagta taagtcctat ttatttcttt ttttaattct tctttatttt tatatgttct
     1381 cattatatta cctccataaa ttcctaattg tctcctttaa ttcatctata atttcatcca
     1441 cgatatttta atccgttcta caatgcatat ctgatatgca ttgactgtct aactcatatg
     1501 tgaatgattt ataaaatatc ccaacgtctc atctaaagaa atgttgatcg tcagcaagga
     1561 gctaaaaaat acgaatatat ttcttttgta gtaaaatcgg actggtttgc atatcatttt
     1621 cttcgtaaat atcaaaatca tgttaactac tatcaattac taataaaaac taccacttta
     1681 agcgttaaga actgttaagt ttagttaagt gaggatagtt cacaaaatcc attgttaaac
     1741 ttcacgaaag tggtaaaact caaaatgctt tatataggat tacagcatcg ctctttcttc
     1801 gattgcgcgt tgggtaaaaa atattatgaa gtaaaaattg acgatgacac gttccttacg
     1861 gctcatcaga tgaaacagct acaaaaacga aatgatcaac tcgaagaaaa tttaattcta
     1921 aaaaagcgat tgccatattc acgcctcgct cgacgaacta ttaaaggtca agatttacct
     1981 gaagatcatc ctttttagga aattgaagct ttttatgttg cacaatgcat ttataatact
     2041 acaatgatgt tttcaccaga tattcttcta gggtgcggtg taatgaaaca gatacacttg
     2101 caaaagaaaa ttcaccgcga atttactaaa attatgaata attatgttaa tcttccgaaa
     2161 tttttttcca aaattaaggg atcaagctgg tgttatagat tgtttaaggt tggcaaaaaa
     2221 ataacttctt tttaaacaat tatagaaaac cgatactctg ttaagttggt tgacacggtg
     2281 tcgattttta tgactttctt attctgaaac ttcactaacg gacagtacct aaaaagtcta
     2341 tagtatggac gtttacgagt actattttat caaaaatgct gctcatttca acttatatgt
     2401 tttagtgaac tacccccact tagcttggat aggctacaat taactagaca gaaaaattaa
     2461 ggtgtgtaga ctagaagaaa acataccagg aggaattttt atgtctaaga gaacacgaag
     2521 aactttttca caagaattca agcaacaaat cgtcaatctt tacttagctg gaaagccacg
     2581 tgtagaaatc attcgagaat atgaactaac
//
LOCUS       DADVXU010000183         2588 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.191, whole genome shotgun sequence.
ACCESSION   DADVXU010000183 DADVXU010000000
VERSION     DADVXU010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.191"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            18..1025
                     /locus_tag="KB953_002841"
     CDS             18..1025
                     /locus_tag="KB953_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBB6867746.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1037..2041
                     /gene="neuB"
                     /locus_tag="KB953_002842"
     CDS             1037..2041
                     /gene="neuB"
                     /locus_tag="KB953_002842"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HBB6867747.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2038..>2588
                     /gene="neuC"
                     /locus_tag="KB953_002843"
     CDS             2038..>2588
                     /gene="neuC"
                     /locus_tag="KB953_002843"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HBB6867748.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNR"
BASE COUNT          947 a          333 c          518 g          790 t
ORIGIN      
        1 cataggagga gatataagtg tttaaaggaa aaacaatatt agtaactggc ggaactggtt
       61 ccattggaag tgaaattgtt cgtcagcttt taaaatatga accaaaagct atcagaattt
      121 atagtagagg tgaagaaaaa caattttata tgcaacaaga gctctcaaat cacactaatt
      181 tgagattttt aattggtgat atcagagact ctcaaaggat tgattatgca tgtcgtgatg
      241 tggatattat ttttcatgct gccgcaatga aacacgttcc agcaagtgaa tataatccta
      301 tggaagctgt aaaaacaaat gtgatgggaa ctcaaaatgt aatagatgca gcgatcaaaa
      361 ataatgtaaa acattttgta ggtatcagta cagataaagt agtgagtcct actaacacca
      421 tgggtgcgac aaagttattg agtgaaaaat taataatagc tgccaatcag tataaaggaa
      481 aaacagaaac aatattttct tgtgtaaggt ttggaaatgt catggggtca agtggatctg
      541 ttattcctct ttttagtaaa caaataaaaa acaataaaga tattactttg acagatgttg
      601 atatgacacg atttatgatg tcaataccgg atgctgcaag attagttctg tctgctgcta
      661 tgaattcaaa atttggagaa acatatgtct taaaaatgcc ggcaatcaat attttaactt
      721 tagctcaatc attaataaaa atatataatg atacacatga tgaaaaatat agaggtgaaa
      781 taaaagttgt aggtatcagg ccaggagaaa aattatatga agagctaatg actacagacg
      841 aaattgaacg ctcatatgaa aatgataaaa tgtatatcat tccaccacac tatcatgcta
      901 ctgacgaact gtacaaagat tatgaaaaaa ttaaaatcac tgcatactct tcagatgcaa
      961 cagaattaat gagtggacag gaatttatta gaatcgcaaa agagaataat gtcgtgactt
     1021 ggtaaggagt tttatgatga aatcagaagt atatgtaatt gcagaaattg gtgtaaacca
     1081 taatggaagt ctagatttag ctttagaaag tattgataaa gctaaagaat gtggagctaa
     1141 cgcggttaaa tttcaaacat ttaaaacgaa taagctagtt tccaaaaagg ctccaatggc
     1201 aaaatatcag aaggataatc ttaaagagga cagttctcaa tttgaaatgc taaaaaaatt
     1261 agaattatct caatctgatt ttattagaat aaaagattat tgtatacaaa ataaaattga
     1321 ttttttgtct actccttttg atgaagaaag tgcagacttt ctaaaacaaa ttggtgtaca
     1381 aggatttaaa attggctcag gagatttaac aaatttacct tttcttagaa aaatagatat
     1441 gtatgggttg ccagttttat tatctacagg tatggctgat attcatgagg tcagtgaggc
     1501 tgtagattgt ttcatagata gtccagttac tattttacat tgtacttcaa attatccggc
     1561 tagttctgaa gatattaatt tattggcgat taacacatta aaagaaatgt tcgggatacc
     1621 cgttgggtac tccgatcatt cattgggata tgatgttggt atttgtgctg ttgcgatggg
     1681 tgctaaggta attgagaaac attttacctt ggataaggaa ctacctggtc cagatcataa
     1741 agcttcttta gatcctcagg aatttgcaga ttttgtaaag catattagaa acactgaaat
     1801 atttttaggt gatggtgtta agcgagcaat gccttctgaa cttagtacaa aagaagtatc
     1861 aagaaaaagc ctagtggtca ctgagaattt aaatgtggga gatattctta cagagaaatc
     1921 tcttacaata aaacgtcctg gaaatggaat agctcctaag tttttatatc aatatattgg
     1981 caaaaaagtt aaaagaccaa taagtgcaga tagtgttctg cttgaggaag atgttgaatg
     2041 aaaaaaatac ttatagtaag tggaacacga gctgattatg gaatatataa acctgttgct
     2101 gagaatttac tacaagataa ttgggatgta tcatttgttg tttctggaat gcatttatcc
     2161 cataaatatg gatttactaa agaattgatc ttagctgacg gatttagaat tagtggagaa
     2221 gttcaaagtt tgtttttaga gaacaataag ggaaacatgg ctcggtctgt ggcattagag
     2281 ataaatggtt tcacaaatat atttgaacaa actagccctg attttgtatt attactaggg
     2341 gatagaggag aaatgttggc agctgcgata gcttgtttgt atcttggaat caaaacagca
     2401 catattcacg gaggcgaagt gtctggtact gtagatgaat caataagaca tgccataacg
     2461 aaaatagcat ctgttcattt tgctgctacg gaaaaaagca aagagcgttt aataaaaatg
     2521 ggggaagatt cttggagagt ttttcccgtc ggagctccta gaatagatac tattttaaat
     2581 aatagatt
//
LOCUS       DADVXU010000184         2566 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.192, whole genome shotgun sequence.
ACCESSION   DADVXU010000184 DADVXU010000000
VERSION     DADVXU010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.192"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..78
                     /locus_tag="KB953_002844"
     CDS             <1..78
                     /locus_tag="KB953_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HBB6867749.1"
                     /translation="LELFFAVFLFLGKNSLKQTKKKELV"
     gene            complement(108..617)
                     /locus_tag="KB953_002845"
     CDS             complement(108..617)
                     /locus_tag="KB953_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HBB6867750.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(729..1727)
                     /locus_tag="KB953_002846"
     CDS             complement(729..1727)
                     /locus_tag="KB953_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBB6867751.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1928..2512
                     /locus_tag="KB953_002847"
     CDS             1928..2512
                     /locus_tag="KB953_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBB6867752.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
BASE COUNT          801 a          475 c          483 g          807 t
ORIGIN      
        1 ttagaattat tttttgctgt ttttcttttc ttaggaaaaa atagcttgaa acaaacaaaa
       61 aagaaagaac tcgtctaaat agacgttttc tttctttttt tgttgaatta ttgagtaagg
      121 cgacttcttt tttctggttc tttgtgtgct aatcgactgg cagcagcagc ggcgatcgct
      181 ccgacgatat catctaagaa ggtatggaca tgaacgccat cgtggctgtt tagttctttt
      241 aaaataccag gtttcacctt gtcgatataa ccataattcg taaagccgat cgtaccgtaa
      301 acattcacga ttgacaaggc taggatctca tcgataccgt aaaggccttc gtcatcttcg
      361 ataatatgtt gcaacggact catcaatttt ttcttttctg ctaatatatc taattggatt
      421 ccagtgataa tggcgttatg tacttcacgc tttgttagca cgctgttgac actttcagta
      481 caatccgcca aagtcaaatc agaaatatag tctttttgta aaaacatgac aagttgtgcg
      541 atatcttcta cagttacgcc acgttccttc aatagttctc ttgccttttc ttctaaagtc
      601 tcagttttga cgaccatatt attgctcccc ctttattttt tctttctcta tttcatgtta
      661 acggaacatg aaaaaaatgc aagaaactgc tttcgggttt aacttgaaat gcaaagagat
      721 ataaaaaatc aactaggacg aagattttta tcgtataagc ttgtgctgtg accaggctga
      781 gttcgttcct ttggattaat atagtgtaaa gcgttgttca ctgcagtagg tgcttcaccg
      841 agcccagtag caatcagttt cacttttcct tcataggtac agatgtcacc agctgcataa
      901 acgcctggaa tagaagtaga catatctgat tttacagtaa tggcatttct ggtactatca
      961 agtccccaag aagaaagatg ttctaaatta gaagagaatc cataattgac gatcagagaa
     1021 tcaatcatca aatcaatcgt ctcgtcactt ttcacttttt ttaaccggat atctgtcagt
     1081 tccccattat tgcctgataa tccatcgatg agatagggag taagcaagtt gacagatgaa
     1141 gatttcaagc gtgatacact atgttcgtgc gcacgaaatt ccgggcgacg atgaatcaag
     1201 taaacttcgc ttgcaatagg ctctaacatc aacgtccaat cgatagcaga atcaccacct
     1261 ccagcaatag cgactttttt tccagcatac ttcatcaaat cattcacgaa ataatccaat
     1321 ccgtggtttt caaatgattc agcattatct aaattcaatt ttcttggctg gaatgaacca
     1381 tttcctaaag ctaaaatgac tgctttggaa taatggatgc ctttatttgt agtaatctcg
     1441 ataacttcgt cttcacgagt aagcgagaga acttcttctt ttaaatggaa tgtatggtta
     1501 aaagtagtta attgtttttc taattgatcg attaattcac ttgcttttat tgctggatac
     1561 ccaggaatat cgtaaatata tttttcggga tacaaagttg ctaattgacc accaagttga
     1621 ggtagactgt caatgatttt tgttttagct tggcgcattc ctgcgtaaaa agcggcaaac
     1681 atccctacag gacctgcacc gatgattgta atgtcataga tttccataaa aattcctccc
     1741 ttttcacact atatcagaat ttaattgaga atgcttctca aaaatgtttg aattcgaaat
     1801 cgaaacggta agttatagaa tgtatctagt gatgaaaaat aaaagaaaac aaatctagta
     1861 tttttacgag aggttaggta ttatgttatt attattatga agtatgcaaa tattaggagg
     1921 aaacaatatg gcattcccac aactagattt aaaaagtgaa aaaggaccaa aagctgttat
     1981 caaaacaaac cgtggtgaca ttactgttca attattccct gaactagcac cgaagacggt
     2041 tcaaaatttc attgaacttt ccaaaaaagg ttattatgat ggcgtgattt ttcaccgcgt
     2101 tattcctgat ttcatgatcc aaggcggcga tccaactgga acaggaatgg gtggcgaaag
     2161 tatttatggc gaaagttttg aagatgaatt cagtcgtgaa ctgttcaacc ttcgcggagc
     2221 tttatctatg gcaaattcag gtccaaacac aaacggaagc caattcttta ttgtaaataa
     2281 cacaaatgtg ccagctaata tgcttggaca actagaaggt gctggattcc catcagaaat
     2341 cattgaagcg tataaaggtg gcggaactcc ttggctagat ttccgacata cagtctttgg
     2401 acatgtacta gaagggatgg acacagtaga tgaaatcgca aatgtacaac gtggtccaca
     2461 agaccgacct gtacatgatg tagtgattga aacaattgaa atttcagaat agtttggtat
     2521 ggtacggagt aattatgaaa aaggacgaaa cctagtaagg tctcgt
//
LOCUS       DADVXU010000185         2446 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.193, whole genome shotgun sequence.
ACCESSION   DADVXU010000185 DADVXU010000000
VERSION     DADVXU010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.193"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(4..249)
                     /locus_tag="KB953_002848"
     CDS             complement(4..249)
                     /locus_tag="KB953_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867753.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(266..1117)
                     /locus_tag="KB953_002849"
     CDS             complement(266..1117)
                     /locus_tag="KB953_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBB6867754.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            1283..2287
                     /locus_tag="KB953_002850"
     CDS             1283..2287
                     /locus_tag="KB953_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBB6867755.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT          786 a          416 c          493 g          751 t
ORIGIN      
        1 tttctatttt ttcggtatca aggcaaaaac aggtagttca ggtgaataaa tgacttcatg
       61 ttcgtgccag ggtttgacca ttagcgaatg atcgaaccgt tttttcagtt cttcgatgat
      121 ttgatcatgt gtccagtttc ttgcaggaaa atgttggatt ttctctgcat cgatcaaaaa
      181 gacaaaatcg gaatgaaaag tctgggtaac ttcttccgag agagaattca agcgttcgat
      241 tggatccata aaatcacctt tcgacttaaa attgggtagt atctgggtct tttgctttgc
      301 cgactacacc atctaactga tcgatttgtt tcatttcttc ttctgttaat tcaaaatcaa
      361 ataattcagt attttctttg attctgcttg gtgtcactga cttaggaaga ggaaggaatt
      421 cgtgttgcag cgaccaacgc aaagcaactt gtgcaatcgt cttgtcatgt gcttcagcga
      481 tttgtttcat ttcaggcaca tcgaagattt taccagtgcc taatggacta tatgcttcga
      541 ggatcatatc atgttttttt gcatactcaa ctacagttgt ctgtaattcg cctggtgcaa
      601 ggaaaacttg attgaccatc ggcataattt ttgctgtttt tgctagtgta tctagatggt
      661 ggggtaagaa attgctcaca ccgattgctt tgattttacc tgcttcatac agttcttcaa
      721 atgctcgcca tgtatctgca tttgcttgtt cccaattatc acgaaaagca acagggtttg
      781 gccagtggat caaaaacaga tctaaataat ctgtttggag cttcttcaat gattcttcaa
      841 aagaagacat cacaagttca tagctgtggt ttgcattcca taattttgtt gtcaagaata
      901 tctcttctct aggaattcca ctttccttaa tcgcttggcc gacgctttct tcattcttgt
      961 atccttgggc agtatcgata tggcgataac ctgcagccag cgcttcttta acagaggaaa
     1021 cagcgacatc cccatcaggt gtttgccatg tgccgaatcc tactaccggg atttcgtaac
     1081 catttgatag acgataagtg cttgttaatg aattcatact ttcagctcct tttttattcg
     1141 ttcaactata agtgtagcgc atttttcgaa aaaaagaatc cattttgttt ctactcttga
     1201 aatttttgtt taaaagaaaa tgattttgat tctggtattt ttgatttttt ttccgtaaac
     1261 taagacagaa gggaatgaga aaatggatat ctatttaaaa aaagcaattc tgcatattat
     1321 tgacagagaa actggttctc cagtcttttc acaaaaagag ctggatctga caaaagaata
     1381 tattcgtgat tttcttcaaa agaagatcca aaaaatctct tctgctcaaa caaaaactgg
     1441 acaactagca gaagaaagca tgatcgttga atcacttaaa atcgctcaag aaaattttgt
     1501 ggaagcaagt gaagcaatcg ttcagcggtg gttcgatatc taccaagaaa gtgaagaagc
     1561 acctagtgca gatgtatttg ttgtcttgta tgaattagat acagtgatgt acttatcttt
     1621 attgaaagtc aattatcgtg aggcttacac tcactttgtt gaagcagacg aagcagggat
     1681 cgacaaccgc ttgattttga atcgagcaat tctaggggcg aaaacacaaa aagcagatga
     1741 agcattcgct gtgaatttga ctgattggac ctacgaatta attgaaaaaa aatatgagtt
     1801 ttctggcaaa aaagagcttt atttttctgg aagagtgatc gaaagtgttc ctgcaccttc
     1861 tttagaagaa aatgtaaaag tgatccaaaa agtagccaag catcttggaa aaaaatttga
     1921 gacagaagaa tttgatatca tggctgattt gaaagaagct gtctatgata cgatagaaga
     1981 aaaaggtcgg ttggatcatg agatgattgc ggaaaaagtc ttcaaggaaa acatcacggc
     2041 aaaaatggct tttaaagaag aagtacaaga gcaaggattt gtcccagaag cacctcctgt
     2101 aaaagaagta caagaaattt ctgagaaaaa atttggaaaa caaaaattca agctatcaaa
     2161 cgggatcgaa ctgatcgtac cagtagatgt atatcgcaac ccagatttga ttgaatttgt
     2221 gaataatccg gatggtacga tatccgtgat gatcaaaaat gtggatgaag tattgaatcg
     2281 tttataaaaa tgactagaat attaggtgtt caagtaccag tgatcagtta aatgaattgt
     2341 gtcaagagaa ggatcggaaa agacgaatat aacaaaaaaa gagcgtgaga caaaagtaaa
     2401 gaacactttt gtctcacgct ctaaacacgt ataaacggcg gaagca
//
LOCUS       DADVXU010000186         2405 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.194, whole genome shotgun sequence.
ACCESSION   DADVXU010000186 DADVXU010000000
VERSION     DADVXU010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.194"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(287..649)
                     /locus_tag="KB953_002851"
     CDS             complement(287..649)
                     /locus_tag="KB953_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867756.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="KB953_002852"
     CDS             complement(723..1103)
                     /locus_tag="KB953_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HBB6867757.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1167..2360
                     /locus_tag="KB953_002853"
     CDS             1167..2360
                     /locus_tag="KB953_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBB6867758.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
BASE COUNT          777 a          443 c          387 g          798 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt
     2401 tcgtc
//
LOCUS       DADVXU010000187         2399 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.195, whole genome shotgun sequence.
ACCESSION   DADVXU010000187 DADVXU010000000
VERSION     DADVXU010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.195"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            207..2069
                     /locus_tag="KB953_002854"
     CDS             207..2069
                     /locus_tag="KB953_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBB6867759.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2399
                     /locus_tag="KB953_002855"
     CDS             2069..>2399
                     /locus_tag="KB953_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBB6867760.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatt
//
LOCUS       DADVXU010000188         2395 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.196, whole genome shotgun sequence.
ACCESSION   DADVXU010000188 DADVXU010000000
VERSION     DADVXU010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.196"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            316..570
                     /locus_tag="KB953_002856"
     CDS             316..570
                     /locus_tag="KB953_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBB6867761.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="KB953_002857"
     CDS             571..888
                     /locus_tag="KB953_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBB6867762.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1447..2382
                     /locus_tag="KB953_002858"
     CDS             1447..2382
                     /locus_tag="KB953_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867763.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          861 a          339 c          453 g          742 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 ggcaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacatc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
     1501 actactttca aaagattatc cgagttaatg cagcagactg ttaaaagtgc tatgcctgaa
     1561 ggatacaagt ttaattttgt tcaatcttca gtatctactg aaagtatcta tttttcaatt
     1621 ctttctgaga gtaggctttt tcattttaga ataagttctc atgtaaagaa aactgatgtt
     1681 gatatatata cgttccgtac aaataattat aaaaatttaa atgatttgag tagaagtatc
     1741 agtgaatatc tcaaggggaa tagctatctc aagattcgat tttcacatat attaatttta
     1801 ctaccatatt tgaatgagaa tttcacccta attcaagtag ataagtcttt cattgcctca
     1861 ggtaatcaag gaaatatgaa tattacaagt aacaaaacgt cacgtgtgct attggcgttg
     1921 atgggttacg gaattcttta tagagatgac caaaatggcc agattctaat aaatcgggct
     1981 gggcgttcat tattaatgaa atatagaaaa tttataccgg aagattttca aaacttacag
     2041 ttagaaaaca tgcgtttaga agatttattt agattagcag aagatgataa gtatagattt
     2101 gaagttttga aaattcaaaa gaatgaacta ttgaataagt tatatcctga gaatgcgata
     2161 aaagcacatt ttggtgatgc taatcatatg tatagttggt tcattcctaa aagatttaga
     2221 gggaaaatca atgctggaga tcaagtgaaa gtactcaatc gaaatagtac agatgtcgta
     2281 atgataacag atatctatgt agaagatgaa gatataatca aatatatgaa gcctgtgata
     2341 aaacttgtta ataaaaataa aggaacccgt ggtgctagtt aattttttcc tagag
//
LOCUS       DADVXU010000189         2323 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.197, whole genome shotgun sequence.
ACCESSION   DADVXU010000189 DADVXU010000000
VERSION     DADVXU010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.197"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            7..1107
                     /locus_tag="KB953_002859"
     CDS             7..1107
                     /locus_tag="KB953_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBB6867764.1"
                     /translation="MAKYYNKKILIMANNDDGLYRFRGMLIKNLVDQGNEVIVSLPKG
                     KNIPKLIELGCQIIETPIDRRGINPKTDFKLFFNYCNILKKIKPDMVIAYTIKPNIYG
                     GLACRLRKVSYALNITGLGTAFQKKGVLKSLIVFLYRLAAKKSKVIFFENFENMKTFQ
                     TLQIAKQSKYKLLNGAGVDVEHFSYLEYPKDEQVHFLFIGRIMKEKGVDELFEAMKNL
                     YSEEKNCVLDVLGYFEEDYGIKIEKYEKEGWLKYHGYQSDIRPFVERAHCFVLPSWHE
                     GMANTNLESAASGRPIITSDIPGCKEAVIESKSGYLCERKNSCSLYNSMNKFLKLSYE
                     QRINMGKMGRNYMIKKFNKKKVVDETIKSLFS"
     gene            1114..1509
                     /locus_tag="KB953_002860"
     CDS             1114..1509
                     /locus_tag="KB953_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBB6867765.1"
                     /translation="MFCKVCDRKEGFCGFRIDIKELAKVSDIFLFTTLQEGMPRSMME
                     AMASGLPCIASKIRGNVDLLVEGKGGYLVPAKDAELVAECLRELINNKSLRKYMGNEN
                     LSRIKEFEVSIVKKRIKEIYEEVIGESVK"
     gene            1506..>2323
                     /locus_tag="KB953_002861"
     CDS             1506..>2323
                     /locus_tag="KB953_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBB6867766.1"
                     /translation="MKNPMFSIIVPIYCVEKYLKDCLDSIRNQTFKNFEAILVDDGSP
                     DECPEICDYYSQIDGRLKVIHKKNGGIIDARKTGVTSAMGEYIVFLDGDDWLANTYLE
                     KYAEVITKYNPDIICSGYFASRSNKIRKRELSCSFGYYDKDKIINLIYPKLIEDEHGE
                     YFRPQLWAKAFRTTLYKEQQQVSGQVSVGEDHACTKPCIYHANSIYMMEKCLYYYRIN
                     ENSITKKPQPYYWEGPKAIGEHFEKQIDMNKYDFQDQVYRNVTHNLFNVAVSQF"
BASE COUNT          909 a          262 c          445 g          707 t
ORIGIN      
        1 agagttatgg caaagtatta taataaaaaa attcttataa tggcaaacaa cgatgatggt
       61 ctttaccgtt ttcgaggaat gctcataaaa aacttagtag atcaaggtaa tgaagttatt
      121 gtatctcttc caaagggcaa gaatattcct aaattaattg aacttggatg tcaaattatt
      181 gaaactccaa tagatagaag aggaattaat ccaaaaactg actttaaact attttttaac
      241 tattgtaata ttttgaaaaa aataaagcca gatatggtca ttgcttatac tatcaaacca
      301 aatatatacg gtggattggc atgtcggtta agaaaggttt cttatgcatt aaatataacc
      361 ggattaggaa cggcatttca aaaaaagggg gttttgaaaa gtcttattgt atttttgtat
      421 agattagcag ctaaaaaatc taaggtcata tttttcgaaa attttgaaaa tatgaaaaca
      481 tttcagactt tacaaattgc taaacaaagt aaatacaagc tattaaacgg tgcaggagta
      541 gatgtagaac actttagtta tttagaatat cctaaagatg agcaagttca ttttttattt
      601 attggaagaa ttatgaaaga gaaaggtgta gatgaacttt ttgaagcaat gaaaaactta
      661 tatagtgaag agaaaaattg tgtacttgat gtccttgggt attttgaaga agattacggc
      721 ataaaaattg aaaaatacga aaaagaggga tggttaaaat atcatggata tcaatctgat
      781 attcgtccct ttgtagaaag agcacattgc tttgttttgc ctagttggca cgaaggaatg
      841 gcaaatacta accttgaatc agcagcatca gggagaccaa ttattacaag tgatatccca
      901 gggtgtaaag aagcagttat tgaatcgaag tctggttatt tatgtgaaag aaaaaatagt
      961 tgtagtctct ataattcgat gaataaattt ctaaaattat catatgagca gcgcattaac
     1021 atgggaaaaa tggggagaaa ttatatgatt aaaaaattta ataagaagaa agttgtcgat
     1081 gaaacaatta aatcattatt tagttaaaaa gatatgtttt gcaaagtatg tgatagaaaa
     1141 gagggattct gtggcttcag aatagatatc aaagaattgg caaaagtttc tgatattttt
     1201 ttatttacta cattacaaga aggaatgcca agatctatga tggaagctat ggcaagtgga
     1261 ttgccatgta tagcaagtaa aatccgaggt aatgtagacc ttttagtgga aggaaaaggt
     1321 ggatatcttg ttccagctaa agatgcagaa ttagtagcag agtgtttgag agaactaatc
     1381 aataataaat ctcttagaaa gtatatggga aatgagaacc tttccagaat taaagaattc
     1441 gaggttagta tcgtaaaaaa aagaatcaaa gaaatatatg aagaagttat aggtgagagt
     1501 gtaaaatgaa aaatccaatg ttttcaataa tagtacctat ctattgcgtt gaaaaatatt
     1561 tgaaagattg cttagattca ataagaaatc agacatttaa aaattttgaa gcaatactag
     1621 ttgatgacgg tagtcctgac gaatgcccag aaatatgtga ctattattcc caaatagatg
     1681 gacgtttaaa agtaattcat aagaaaaacg gtggaataat agatgcacgg aaaacaggag
     1741 tcactagtgc tatgggagag tatatagttt ttctagatgg tgatgattgg cttgcaaata
     1801 cttatttaga aaaatatgcg gaagtaatta ctaagtacaa tccagatata atatgtagtg
     1861 ggtattttgc atcacgttcc aacaaaatta gaaaaagaga attgtcttgc tcgtttggat
     1921 attatgacaa agataaaatt ataaatttga tttatcctaa attaattgag gatgaacatg
     1981 gagagtattt tagaccacaa ttatgggcaa aagcttttag aactactctg tacaaggaac
     2041 aacaacaagt atctgggcaa gttagtgttg gtgaagatca tgcatgtaca aaaccatgta
     2101 tttaccatgc aaattcgata tatatgatgg aaaaatgttt atattattac agaataaacg
     2161 aaaattctat aacaaaaaaa ccacaaccat attattggga aggaccaaag gctattggag
     2221 agcattttga aaagcagatt gatatgaata agtatgattt tcaagatcaa gtatatagaa
     2281 atgttacaca caatttattt aatgttgctg tttcacaatt taa
//
LOCUS       DADVXU010000190         2270 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.198, whole genome shotgun sequence.
ACCESSION   DADVXU010000190 DADVXU010000000
VERSION     DADVXU010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.198"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..489
                     /locus_tag="KB953_002862"
     CDS             <1..489
                     /locus_tag="KB953_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HBB6867767.1"
                     /translation="PYSKKEIKELVNVYREHKPDINFLENADTLLTELRSRGYKLGII
                     TDGYAITQRNKLDALKVDYLVDYICITDELGRELWKPHPISYLLLLEKFNLKAQECIY
                     IGDNELKDFITAKSIGMQTIKIERVNGIYRESSLKFKRFYQADKKIRSLWEIKNFLGV
                     IS"
     gene            486..1604
                     /locus_tag="KB953_002863"
     CDS             486..1604
                     /locus_tag="KB953_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBB6867768.1"
                     /translation="MKSKRILLASPHMSEEGYEKEYVKEAFDTNWVAPLGANVNGFEK
                     ELAEYVGASHAAALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPILYQGAIPV
                     FVDSDYETWNMKPEHLRTALEKYPNTKAVLVVHLYGLSANLDEITKICKEYDVTLIED
                     AAESLGTTYKGKYTGTFGDYGIYSFNGNKIITTSGGGMLVSNDKEKVEKVRFWATQSR
                     EKARHYQHNEIGYNYRMSNIVAGIGRGQLKVLNERIEKKRYIHSFYKKELSKLSNIQF
                     MPENNWNEPNYWLSVITIKGINANHLIDELDKNNIEARPVWKPMHLQPVFKNYDYIGD
                     DVAETLFDLGVCLPSDTKMTDEDLKYVVSTIKRVINDA"
     gene            1594..2214
                     /locus_tag="KB953_002864"
     CDS             1594..2214
                     /locus_tag="KB953_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBB6867769.1"
                     /translation="MMRKKTFYQLFGKRILDILLSGIALIVLSPIILIVGFLVRIKLG
                     SPIIFKQERPGKSEKIFSMYKFRTMTDERDHNGEYLPDEIRLTKFGKMLRATSLDELP
                     ELWNILKGDMSIVGPRPLLVEYLPLYSEKQRKRHNVRPGLTGLAQVNGRNAISWEEKF
                     DLDVYYVDKISFFNDLIIIIQTCKKVIKKENINTINNNIPEKFKGS"
BASE COUNT          857 a          295 c          442 g          676 t
ORIGIN      
        1 ccttatagta aaaaagaaat taaagaactg gtaaatgtat atcgagaaca caaaccagac
       61 ataaattttt tagaaaatgc agatacttta ttaacggaat taagaagtag aggatacaag
      121 ctggggatta ttacagatgg gtatgctata acccaacgaa acaaattgga tgctttgaaa
      181 gtagactatt tggtagatta tatttgtatt actgacgaat taggccgtga attgtggaaa
      241 ccacacccta tctcatatct tttattgtta gaaaagttta atttaaaggc acaagagtgt
      301 atatatattg gcgataatga gttgaaagat tttataactg ccaaatcaat aggaatgcaa
      361 acaattaaaa tagaaagagt aaatggtatt tacagagaaa gcagtttgaa gttcaaaaga
      421 ttttaccaag ctgataaaaa aatacgttcg ttatgggaga taaaaaattt tttgggagtg
      481 atttcatgaa aagtaaaaga attctattag cttctccaca tatgagtgaa gaaggatatg
      541 aaaaagagta tgtaaaagaa gcatttgata caaattgggt tgctccttta ggtgcgaatg
      601 tgaatggatt tgagaaagaa ttagcagaat acgtgggagc tagtcatgct gccgcattat
      661 cttctggaac agcagctata catttagctt tgaaagcggc tggggtcggt aaagatgaca
      721 tagtgatttg tcagtccttg acgttctcag ctactgctaa tccaattctc tatcaaggag
      781 ctatacctgt atttgttgat agtgattacg aaacttggaa tatgaaacct gaacatttgc
      841 gaacagcact agaaaaatat cctaacacaa aggctgtatt agtagtacat ttgtacggtt
      901 tatcagctaa tttagacgaa attactaaaa tatgcaaaga atacgatgtt actctaattg
      961 aagatgctgc agaatcatta ggaacaacat ataaaggaaa atatactggt acatttggcg
     1021 actatggaat ttattctttt aatggaaata agataatcac aacttcaggt ggcggaatgc
     1081 ttgtatccaa tgacaaagaa aaggtggaaa aagtgagatt ttgggctacg caatctcgtg
     1141 agaaggcacg ccattatcaa cacaatgaaa ttggctataa ttatcgaatg agcaatatcg
     1201 ttgctggtat cggtcgagga caattgaaag tattaaatga aagaattgag aaaaaacgat
     1261 atattcatag tttttataaa aaagaattgt ctaaattgtc aaatatacaa ttcatgccag
     1321 aaaataattg gaatgaaccg aattattggt taagtgtcat aacaataaag ggaataaatg
     1381 cgaatcatct tattgatgag ttagacaaaa ataatataga agcccgccca gtatggaaac
     1441 ctatgcattt acaacctgtt tttaaaaatt atgattatat tggcgatgat gtagcagaaa
     1501 ctttatttga cttaggagta tgtttaccat cagatacaaa gatgacagat gaagatttaa
     1561 aatatgtagt atctacgatt aaaagagtga tcaatgatgc gtaaaaaaac tttctatcag
     1621 ctttttggga aaagaatttt agatatccta ttgtcaggaa ttgctctcat cgttttatcg
     1681 ccgataatct taattgtagg ttttttagta agaattaaac ttggttctcc aattatcttc
     1741 aaacaagaac gaccaggaaa aagtgaaaaa attttttcca tgtataaatt tcgtacaatg
     1801 actgacgaaa gagaccacaa tggagaatat ttacctgatg aaattcgact aactaaattt
     1861 gggaaaatgc ttagagcaac aagtctggat gagctacccg aactttggaa tattttgaaa
     1921 ggtgatatgt cgatcgtagg acctcgtcca ttattagtgg agtatttgcc actatatagt
     1981 gagaagcaac ggaaaagaca caacgtaaga ccaggattaa ctggactggc tcaggtcaat
     2041 ggaagaaatg ctattagttg ggaagaaaag tttgatttag atgtttatta tgttgataaa
     2101 attagtttct ttaatgatct tattattata attcagacat gtaaaaaagt aatcaagaaa
     2161 gagaatatta atacaatcaa taacaatatc ccagaaaaat ttaaagggag ttaaatacag
     2221 cagcttaatg caagttttgt gacttgcatt atatttaggt ataaatcaaa
//
LOCUS       DADVXU010000191         2262 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.199, whole genome shotgun sequence.
ACCESSION   DADVXU010000191 DADVXU010000000
VERSION     DADVXU010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.199"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(151..2148)
                     /locus_tag="KB953_002865"
     CDS             complement(151..2148)
                     /locus_tag="KB953_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HBB6867770.1"
                     /translation="MIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKA
                     LVTNKANQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHL
                     KEATKRLSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVY
                     IKNSGVTDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADE
                     AEKYLAKGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGS
                     NLMLSMNAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHE
                     QGSKEITENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKS
                     SVFNRSGQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRD
                     SFKEFGLGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYT
                     PIQLAQYASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMA
                     ILREGFHQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGP
                     TDNPEIAISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          576 a          505 c          411 g          770 t
ORIGIN      
        1 aatttttgtc acaacctttt ttgccgagta aacggtgttc aaaagccagt ttctcggtat
       61 gaagtcaaat ttaagcaaaa atgtgagaag ctattttcac aaaatttttc ttcatactcg
      121 aaactaagcg gctttttcac gacctctttt ttattttgcc atgaacatat cataatacgc
      181 gtccattatt tctttagcaa tcgtcaagtt tatgtggtct tgttcgtcta ataagttcgg
      241 caagacaaca ctgatggcaa tttccggatt gtctgttggt ccgtatgcca cgatattact
      301 attgactgtc gcaatatctg gatgaccttc cgcaaccgtt tctgcagtcc ctgtttttgc
      361 ggaaaggtcc attttcgcac tggctaatgg tttggctgtt gtgtaaggat cggtaccatg
      421 gacaacttgg tggaatcctt cacggaggat cgccatattt tctgcagaaa tattgaccgt
      481 attttctact gttgtgccaa tttctttctt cacttcaccc aagtttccat tttcgtcatt
      541 gccatagatc gctttgacta ggtgcggctg tactcgtttc ccgccattag cgactgttga
      601 agcatattga gctaattgga ttggtgtata tgtgtcaaac tgtccaaatg ccaaatcggt
      661 aaagttacct ggagcatacc attctttgcc attatccgca ttgaacttct tcatataatc
      721 aacagaacgt gagatcccag gagattcatt cggcaaatcg atcccagtcg ttgtaccaag
      781 accaaactct ttgaaagagt ctctcaattg ctgataagct ttagcttctt catctaaact
      841 aggtagtccc attccaggtg tatagtctag tcctaacatc tttaacgcca ctttgatcat
      901 atacgtatta gaagacaatt ccgatgcttt gactgcatca agagcaactt gtccgcttcg
      961 attaaagaca gagctttttt ctgaagcacc ttgcagacga atcggctcat caatcaaaac
     1021 ttgatttccg ctgatcgcgt tacttgccca gcctgctgtc aaagtaccag ccttgactac
     1081 agaccctgga tcaaaggcgc tagtaatcgt gcctaaagca ttctcggtga tttctttgga
     1141 accttgttca tgactgaaac cagtcattgc tagcacttct cctgtctgag gattcatagc
     1201 cactgcataa gcaccaggcg aatactgtgc tttgccgttg ttgatcaatg tttgataatt
     1261 ccttttcaaa atttcttcta cttttgattg gaattctgca ttcatagata acattaaatt
     1321 agaacctttt tccccagaga agatttcttt ttgattcgaa acatttcctt cattatcaag
     1381 ggaaacttca tattgggttt tggttccttg caggacatct tcatattgtt tttccaaata
     1441 actttgtcct acacggtcat tccgagaata accttttgct aggtatttct ctgcttcatc
     1501 tgctggaagt ccttgctttt cagtcgtcac actgccaaga atacttttca gtgaacttgc
     1561 tgcattatat tctcgtgtcc agtctgtgcc agtcgaaact cctggaagtt cagaagcgcg
     1621 ttctgccaca acagctaact cttgatcagt aacaccagag tttttgatat agacagtgtt
     1681 taattcatat gcactattca tccgtttaaa gatggtagca atttttaatt gctcctcgtt
     1741 taaattaatc tcatcagctg tgactttatt gaccatcgca ctatattgtt cgctgttccc
     1801 taattgcttt tcttttgccg aaagtctttt tgtggcttct ttcaaatgtt tgggatcagc
     1861 cagccaaaaa tcttttttat ctctatctgt cagattttca tctaccggaa catcgatcag
     1921 cgagttcagc ttcgttgcga tttcaagcaa atctttagcc gtaacttgtg ttcctctcgt
     1981 atatgtgatg gcctgattcg ctttattcgt aactaaagct tttcctgtgg agtcgtagat
     2041 cattccgcga ggagtacttt cttggaccgt aatcgtagaa gtagacttca ctcgcgcctc
     2101 catctcttcg ccgttcacga tctgcaaata acccaatcta gcaatcaacg caacaaataa
     2161 cgtaaatata atgaaaaaaa gaaaatttaa gcgaaaagga acatgggacc ggcgaaacgt
     2221 tttgttctta ggttcttgtg caccttttct tattttttct aa
//
LOCUS       DADVXU010000192         2233 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.200, whole genome shotgun sequence.
ACCESSION   DADVXU010000192 DADVXU010000000
VERSION     DADVXU010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.200"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            96..>2233
                     /gene="acm"
                     /locus_tag="KB953_002866"
     CDS             96..>2233
                     /gene="acm"
                     /locus_tag="KB953_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HBB6867771.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAG"
BASE COUNT          873 a          346 c          446 g          568 t
ORIGIN      
        1 agattggaaa tgagtggggg aggaattgaa gaaaggattt ttgagagatg atatagtagg
       61 ttttattttt agttaaaata ggggagatag aaaaaatgaa aaagtgtaga aatattattt
      121 tatcaatgtt atttattatt actaatataa catcactgat tcctgttcat gtctatgcgg
      181 atgcaggcag agatatcagc agtaatgtca cttcgttgac cgtggaccca acgaatatta
      241 cagatggagg aaacataaaa gtaaaattct cgtttgacga aaaaaaacaa aatatccaac
      301 caggtgatta tctttggatc aattggccaa gtgaaggaaa tattcgcggt gaaggattcc
      361 aaaaagaaat cccattaatg attgaaaata aaaatgttgg aactttaacg gtcagaaaag
      421 attctgcgca ggttgtgttt aatgaaaata ttaaaaacct agactcagta gaaggttggg
      481 ggcaatttga gatacaggcc agaaacgtaa ccgataccgg tgaagaaaat acgggaccat
      541 tcaccgtgac gagcggtgat aaaacagcta ctgtaaatgt gacaaagcca gcttctggtt
      601 cttcttctag cgttttttat tataaaacag gagacatgct acctgaagat actaagcaca
      661 ttcgatggtt tttgaatatt aacaataacg gcacttatgt tgaacaacca gttaaaatca
      721 gtgatgaaat ccaaagtgga caaagattag atccaagtac gtttgaaatt aatcaaatac
      781 acttaggtga acagaaggtt tatagaggag aagagggaat ccaacaattt ttacaagatt
      841 tccccagcgc tacatttaat ttttcagtaa ctgataatta cattgaaata actataccaa
      901 aaaactttgt caatcttagg aaaataatgg tgagttataa gacgatcata gaaaacccag
      961 aacaaataaa ttttgagaat cattcggaag cttggtttaa agaatttaat aaacctgctg
     1021 ttgatggaga aagtttcaat catacagtta aaaatatttc agcttctggt ggagtcaacg
     1081 gtacagttag aggcgaattg aagattttca aatatatcaa tgacacagag attggtatcc
     1141 caaatgtgac tttcgaatta agacgagcag atgaacagcc tattcaagga cagtctagta
     1201 tcctgttgac ttcgaatgaa cagggagaag ctagtatcaa aggcctgcaa gtaggagact
     1261 atgttgtaaa agaaaaagaa gcacccaatt ggattgattt tgatccacta agttcaaatg
     1321 aactcaaatt ctccataaac gaaaatgata cagagggagt cagtttacct atttataaca
     1381 agaaaaaggt tacgaacatc acagcaacta aaatttggaa cggtggaacc actccaagac
     1441 cgagtattta ttttaaatta ttcagagcaa gtacaaacaa ttgggaacca gttcctgatg
     1501 cagaaacaaa acgattagac aatggaataa cttcagtgac ttgggaagat atccaacagt
     1561 atgatgatag tggaaacgaa tatacgttta aagtgcaaga agtagatcaa aacggaaatg
     1621 attatgtccc ttcaggttat caaaaaattg aaaacgggct agtcgttaca aatgagaata
     1681 aagaagtcat ttcagtctca ggacaaaaaa catgggaaga caatgagaac caagacggca
     1741 aacgaccgac gacgatcaca gtaaatctat tagccgacgg tcaaccgatc caacacaaag
     1801 aagtaagtga aaaagatgat tggcgctatc gtttcacaaa tctgccaaaa tacagagacg
     1861 ggcaagaaat catctatact gtaacggaag acaacgtgcc cgaatacagt acgacgattg
     1921 acgggtataa catcaaaaac agttatacac cggaaaaaac aaatatatct ataccaggta
     1981 gccatataac accgtggaaa aattttccag agaagacaga gagagagaaa attagaaatc
     2041 tattctctca aaaattgcaa atatttgctc atacaggaga atccagcgaa caaagaagca
     2101 attatattag taagagagct gtacctaaac agataacaaa taaatcaaaa agcattcttc
     2161 ctaaaacaag tagtaaaaaa tctagttttg ctgtaatcat tggtttattg atagtaacta
     2221 tctgtgctgg gat
//
LOCUS       DADVXU010000193         2221 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.201, whole genome shotgun sequence.
ACCESSION   DADVXU010000193 DADVXU010000000
VERSION     DADVXU010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.201"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            27..467
                     /locus_tag="KB953_002867"
     CDS             27..467
                     /locus_tag="KB953_002867"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBB6867772.1"
                     /translation="MVPRIFGLTPYIIEDKEMSPYYAQGNLIYVRPKNTNEILVGDII
                     TYYENSGKKISTRRVIAVENNHEDFYTKADTKTYIEPGVVKKRNIIGSPIFQIPYIGL
                     VLSKTAFAWIQGLFFTIAFCLTGITAWNTFVELKKKRRRTQKFL"
     gene            595..1278
                     /locus_tag="KB953_002868"
     CDS             595..1278
                     /locus_tag="KB953_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HBB6867773.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1587..1660
                     /locus_tag="KB953_002869"
     tRNA            1587..1660
                     /locus_tag="KB953_002869"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1621..1623,aa:Arg,seq:tct)
     gene            1819..2178
                     /locus_tag="KB953_002870"
     CDS             1819..2178
                     /locus_tag="KB953_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HBB6867774.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
BASE COUNT          738 a          346 c          452 g          685 t
ORIGIN      
        1 ttttaattct tattcttctc gtcgtcttgg ttccccgaat ttttggtttg actccttata
       61 ttattgagga caaagaaatg agtccatatt atgcacaagg aaatctgata tatgtacgac
      121 cgaaaaatac aaatgagatt ttagtcggag atattattac atattatgaa aattcaggaa
      181 aaaaaatctc gacgcgtcgc gtaatagccg ttgaaaacaa ccacgaagat ttttatacaa
      241 aagctgatac aaagacttat attgagcctg gagtagtaaa aaagagaaat attatcggta
      301 gtcccatctt tcagattcca tatattggac tagtcctttc aaaaacggca tttgcatgga
      361 ttcaggggtt attctttacg attgcttttt gcctaacagg aatcacagca tggaatacct
      421 ttgtagagtt gaaaaagaag cggagacgaa cacagaaatt tttgtaagaa tgttaaaaac
      481 ataaatgtat tccagagatc caacgactct tctgaacatg aaacacgaga aaacactcat
      541 gcagcttgta gatttagtca gttgttcgta taatgtgtga agaggaggag tagtatgcgc
      601 aatgaaatga tgcatcgtcc tgtagtcaaa gaagaactat tggattttat gcgtacccag
      661 caaaaaaaat taccgggtga gctaggcaaa ctggaagaag aagctcatgt agcagaagta
      721 ccagttattc cccatgaaac agttgtattt ttgaaatttt tacttggaca gcttcagcct
      781 gaacgaatct tggagatcgg agcagctatc ggtttttcat caagtgtcat ggccacgaca
      841 atcggagaaa acgctcatgt tacgacaatt gatcgtttcg atgtcatgat cgagaaagct
      901 aaaaaaaact atgaacgtct aggactgaca gataaagtga ctttgcttga aggtcaagca
      961 gcagatatct tgccggagtt gtctggtccg tatgacttta tttttatgga tagtgcaaaa
     1021 tcaaaatata ttgaattttt acctgaatgc ttacgtttgt taagaaaagg tggcgttttg
     1081 atggtagatg acatcttcca aggaggaacg atcttgctcc cagatgaaga gattccccgt
     1141 ggcaaacgag ctatccatcg taaattaaat gaatttcttc gtgttgtgat ggatcatccc
     1201 gatttgacat cgacaatctt gccattaggt gatggtgtga tcttaatgac aaaggaatca
     1261 gaaacaatcg agttgtgata catgattatg gcagcctgta gataatcgat tgaatcgagg
     1321 gctcaattcg aacttgatgt aaaagtttct atatttgtaa gtcaattaaa ggatacagga
     1381 aatctaaata aacaggagta gcatgaagtc aatgtagctc ctgttttcaa ttgataacct
     1441 tagaacagct gaaggagaat gtctcgtttt aaaagattga tttagtagtt gggccaaaat
     1501 aattcattat ttgctaatta aataactatt tcgtattgac agaagcaatt taattatcta
     1561 taattattga tgttccgctg attcttgtca tagtagctca gctggataga gcatccgcct
     1621 tctaagcgga cggtcggggg ttcgaatccc tcctgtgacg ctaaaaaacc gttagaaact
     1681 gaggtttcta acggtttttc tttgtttttt gtgccaatat tgtttgtttt caaaggcaaa
     1741 taaaataaaa aaacatcctt atcgagtaca attataagga gcaaaaaaat aaaactcatc
     1801 agaaaagagg aagaagatat gacgatacta tctagaattt tagtgactct agttgcaatt
     1861 gaattttttt atatcatgta tatcgaaacg gtgagaactg attctgacac gacaagtagg
     1921 gtttttaaaa tgagtaaaga agagttaagc agaaaatctg ttcaaacatt gtttaagaac
     1981 caaggagtgt acaatggact attaggagtg ggactacttt atggagctta tctttcttcc
     2041 gcatctaaag aaatcacaag tatgctatta atatctattt tttttgttgc attatatggc
     2101 agtttgacta gcgatcgttg gattttactc aaacaaggtg ggctagctct gttggcattg
     2161 ctttctattt tcttataaat atagaaaaaa taagaattta tctaacagga aactttgtga
     2221 a
//
LOCUS       DADVXU010000194         2159 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.202, whole genome shotgun sequence.
ACCESSION   DADVXU010000194 DADVXU010000000
VERSION     DADVXU010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.202"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            76..642
                     /locus_tag="KB953_002871"
                     /pseudo
     CDS             76..642
                     /locus_tag="KB953_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1023..1202
                     /locus_tag="KB953_002872"
     CDS             1023..1202
                     /locus_tag="KB953_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867775.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            1333..1602
                     /locus_tag="KB953_002873"
     CDS             1333..1602
                     /locus_tag="KB953_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBB6867776.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            1595..1852
                     /locus_tag="KB953_002874"
     CDS             1595..1852
                     /locus_tag="KB953_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HBB6867777.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
BASE COUNT          782 a          312 c          432 g          633 t
ORIGIN      
        1 cgtcattcct cctgctatct ttttctatta ttctacctta tttgatagta gatttaaaac
       61 tttgcaacag aacccgaaga acaattgtat ttggaaagtg aaacgagtca aacgaaggtc
      121 acagtcagcc ataatcgttt agacgtgtat aaacgtcgtc ctgaaacggt aaaaaaagga
      181 acggagcttc tacgactaga aacagaaaaa tacatcatga atgtttacct agaaaggagt
      241 gagctggtcg aaaactttgt cagctacacg atttgcagga aacactatta ctcagaatca
      301 gaatattatt caatttttga gagcatggaa ttaatggaag aagaaatgga agagctaaac
      361 agcaaagatg cgacgataga cgattattac cttgtattta ctagattttt gacgtgtgga
      421 acggaatcta tcgtgttaat gggaatggat tatgaactcg ctgtacccga agcatttttg
      481 agggaatatg tagaagaagt agataaccca ggcgacgact ggtacacgac tttaactgat
      541 tttttagaca cctatactta cgacgataca gaagaaatcc aaaatgaact tgaccgtaaa
      601 cgaatgccat atcaacgatt tgaaaggaaa aaaaggcaat aatcatttat accgaaaagt
      661 gagaatgtgt gaaataagga caaaaaacct agtaactaag ggaatggttc ttatttcgca
      721 ccaatcgctc caaccgcccc cgccgcttag gtggtgcgaa ataagcgggc aacacccgat
      781 aacctaagag aaagaaggag atccatatga ataaaatcat tcctctattg ttagtcctat
      841 ttgttggcga aatacttatg cgccatgctg cagctgtgca tttaaacttc gtagtatttt
      901 tggcaatact ggtattttgt ttgatctttt ggcgctttgt attagccctt gtgagtctac
      961 tattcaagat gattgcagcc atatttttca tagggttatt tcgatgaaag gagagagtac
     1021 taatgccaat tgcaatagga aataaacggt tacccgttac gttggatgaa aagagacaaa
     1081 aagaattgca gcaactaaag cagaagtacg gcaaaagtga atccaggatt atgtgtattg
     1141 cgttagattt attgattgcc caagaaaaag caggatttga ggtaccagca ctcaaaaagt
     1201 gacgtcactt tttatcctaa aaactaaaag tgatagcact tttaattata agaagttaga
     1261 atattaatca tttgcttaat tgtacaatat aatgtacaat tgttttatag aaataaataa
     1321 ggggtgaaag gaatggaagc agtagcttat tcaaatttcc gccaaaattt acgtagttat
     1381 atgaaacaag ttaatgagga tgctgaaaca cttattgtaa caagtaaaga tgtagaagat
     1441 acagttgttg tattatcaaa aagagattat gattctatgc aagaaacgtt gagaacactt
     1501 tctaataatt acgtcatgga aaaaattcgt cgaggagatg aacaattctc caaaggtgca
     1561 tttaaaacac atgacttaat cgaggttgaa tctgatgatt aaggcttggt ctgatgatgc
     1621 ttgggatgat tatctttatt ggcatgagca aggaaacaaa agcaatataa aaaagattaa
     1681 caagttaata aaagatatcg atcgttcccc ctttgctgga ttaggaaaac ctgagccatt
     1741 aaagcatgat ttatctggaa aatggtccag aagaattaca gatgaacata gactgatata
     1801 tagagttgaa aatgaaacga tatttattta ttctgcaaaa gatcactatt aaccaatcgg
     1861 aagtaaggaa agggtcagaa acttaaaagt ttttgatcct tattttattt accctagtca
     1921 tttaaaaagc taatatagct tagtgttgat tgttattaat gaatgtgttt gttacgcgta
     1981 ttacggatat aaggttagta aaatcatttc taaagttgag gaaaagtaaa tataaatggc
     2041 ttaaatttca acaatttgaa gttgaataga tatgttataa tactattgta gtgtgggatg
     2101 ttagttacta aaggatgacg cttatatata tgactgaata gaataagcaa taggtttaa
//
LOCUS       DADVXU010000195         2090 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.203, whole genome shotgun sequence.
ACCESSION   DADVXU010000195 DADVXU010000000
VERSION     DADVXU010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.203"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            703..1035
                     /locus_tag="KB953_002875"
     CDS             703..1035
                     /locus_tag="KB953_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HBB6867778.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1654..1995
                     /locus_tag="KB953_002876"
     CDS             1654..1995
                     /locus_tag="KB953_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HBB6867779.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          734 a          278 c          435 g          643 t
ORIGIN      
        1 aaaatattat gataaaaatc acaaacgtag ccttttacgt cataagtgtt aaaagaaata
       61 attaatttca aatatagaaa agggaacgta tgtttgcata ctcctttttt ttgagttata
      121 ataatacgcg aagagagata atttcattat ttaaacaagc taaaaatctt gatattactt
      181 cttttcaggc aatacgaata ttattcgctt acattttttg tataaattta attgtctgct
      241 atgcttgtga tgaattgatt ttcgttttgc acttttggat gataaatttg gacaattgaa
      301 aatacaaaaa aaagaaaggc ggtatgaact cggaaggctg gtaaccttcg tcagcgttca
      361 tacacggcat taagatttgg cccttaatgc gttaaccttt cagtgttacg gtattacccg
      421 caatggattt agtataacag atattcaaaa gtgataccac tttttttggc tgtgttaagg
      481 ttaaaaatac gtgatattac gatgaatcta tgcctaagca taatgttata aaaatgtccg
      541 tgtggctctt tgccgtatga cgaacgctga aattagaggg aacgcatgtt tggatgcgtt
      601 ccctcttttt tgtttgtcat gaacgaaaat caattctcta tttttgaaaa ataacaagaa
      661 aaacttgtta aaaatattta aagaaattgg gtggtttatt ttatgtcata tacaggaatt
      721 ttgagtctca aagatatttg tcactacggt aaacggtgta ccgctacaga gaaaatcacg
      781 aagaaactat caactggcca aagtaaaacc gttgtgcagt gtaagaaata tataatccag
      841 aaagacaaag tctcagagga aatgatttat tacattggga aacaaaagca aataatttta
      901 aaagatccta tccccttaaa agagctatat ccaacaatca aacatgttta tgaccaaaac
      961 ggtgtactga ttggtcgaag aaaaaatgga gtcttgcgtt gtaccgcaaa agggatgggg
     1021 cgtttgatcg gctgaattga tcattctcat aacttgtatt ttatcgtttt tcgcttgctt
     1081 gaaaatattg tcgagacttc taaaggaact tagagtcact agaaaaataa ggagtagact
     1141 tgatctatca acaattccct tcggggagtg acggggtagg ttttaacgat tggtaacgta
     1201 aaaaattggt caatcgagta aatgcgcttg tttggatagt ggtgaaatga atttgtgtaa
     1261 ataataagcg cagagtaaac aagagggaat ttagaagtta gacaagttag aatagcgtct
     1321 agattaatca tgagcagcta ctaagggatc tagactttga cgcaaactca tggaataagg
     1381 agtaggtctt ctcgtaatgg ggaggacgca caagaaattg tgtcgtgtgc tgcagtacat
     1441 gtagatactt gcgaggacgt tgattattgg atcaatcgag catttaccgc tgtctactag
     1501 ggtttctcgt ttcggacgcc ataaacgagt cgaaattttt tatgtatttt taagtaaacg
     1561 aaaaacgata aaatatgaac aagtcctgaa taaagtgaaa aaattgatat aaaacataga
     1621 gaaaatgaaa tttacgtaat ggaggaatga caaatgaaaa attatgatcc gaatatacga
     1681 ttgggcacac atactattaa ggtttctttc caacgttggg attacaaagg atttgtgacg
     1741 tttagaaggg gcggaaattg taaaggatta gatgttttag cattagacga ggatgattta
     1801 tacgatcaaa cattgactga taatccaatt ggattcggct tgctacctga agatgatgaa
     1861 ggtaatgaat ggtttaaaat gacgcttatg aatgataatg gagacgaatt atctgttgaa
     1921 gatacttggt catatttgag tgattatatt gtcagctttg aaatcattga atttgtagcg
     1981 gataaagaag aataaaaact ggaggaataa attttacaga taaaggctta gaaggagtca
     2041 cgacgattaa taaaaaggta gtattacctt aggcattgag taaggaattt
//
LOCUS       DADVXU010000196         2057 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.204, whole genome shotgun sequence.
ACCESSION   DADVXU010000196 DADVXU010000000
VERSION     DADVXU010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.204"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(565..930)
                     /locus_tag="KB953_002877"
     CDS             complement(565..930)
                     /locus_tag="KB953_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867780.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1006..1251)
                     /locus_tag="KB953_002878"
     CDS             complement(1006..1251)
                     /locus_tag="KB953_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6867781.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1321..1575)
                     /locus_tag="KB953_002879"
     CDS             complement(1321..1575)
                     /locus_tag="KB953_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867782.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1739..2044)
                     /locus_tag="KB953_002880"
     CDS             complement(1739..2044)
                     /locus_tag="KB953_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867783.1"
                     /translation="MPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVITLLLLV
                     MMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINPLIFSR"
BASE COUNT          758 a          357 c          366 g          576 t
ORIGIN      
        1 attaattttc gtctgaaaac aaaaaaagaa agcgttatta ttttagtcta ttttcagggg
       61 gggaacctta tgataaaaaa actattttta ctatcaatat taacgctagc aatggcaggt
      121 tgttctgact ctggcttagc aactgaactg atcacaaaat caactgaagc cacgacagag
      181 gagttaaaat gtggaaacga tcacagtgac tacagaggct taaatcgacg atgcatggga
      241 atattaggca gaagcaattg taagcatcga tgtagtaatc gaagaaggtg tagaagaagc
      301 aacagaaaaa tcaaaagaaa gatcaaaaga agcaatctgt gctgtgcatc gtatacagac
      361 gtcgaagatg cggtactttt aaaccacgtc aggagatgtt catttatgtc tagggaagtt
      421 ggcgatgaac caaaactaga gtacgctttt agttttgcac gtgaggatga agataaccct
      481 aaaacttacg acattttagc ttgatctaag atggttaaag ctttttaatg aagtaagagg
      541 agatagaagg cttttctagt tatattaggt aagataaatt cctttttgct ccaaatttgc
      601 taaacattct gctagataaa cttcatcgtg ttccggataa atcggcacgt tgcgctctac
      661 agtaggtaaa tcaaaaaatg cttcgcatga tttgccacga tacataggat ttttccactc
      721 tggtgctggg ctgtaaccac gtttttccat ttcatccatg acaagctgat gatattggaa
      781 taatctataa ggagaatagt gaaaaacata atccacagta gcatgtttct ttccccagcc
      841 atttcctctt aatgcacagc attcccgatg ttgtcctaaa agctgttgac gaggaagctt
      901 tgaaatcaat ttttcatgcc ataatcgcat agaaatgtct cctttatata tagattgtta
      961 accttagacg ttaaatgaaa aaaccagcac caaggctgga aaaaatcact tcagtttgct
     1021 tgctgtttct gagcgaccaa ttaggtaatc aacggaaata tcgtaaaagt cagcgatata
     1081 gattaattca atgatgcttg gaatttcttc tccttcttcg tacttacgat atttgaataa
     1141 aggaatactt aacgcgctag aaacagctac ttgagataaa gatcggccag ttcttaagaa
     1201 attcagacga caacgtaaag tatccagtaa aatagcttct tcgatactca tttaatcacc
     1261 tttcattata gtagaatagt tataatgact atagaaagct acacatgcaa tcaatagtac
     1321 tcaatgtttt ttatttaaac gtatcaaata aagaatgacg atcaataaag acaaaaccag
     1381 tcctgataat tcttctataa aaacactatc gggttcaaag tagttgtaat taaaaacttt
     1441 ccacataaag tagatagccg gaagagtcaa aatagctaaa taaaaccgtt ctttgttcca
     1501 attcataaga taaacaatac ttgcgaccag tgcgaccgca ggcacccaaa acaaaactct
     1561 caccaacgta atcatgtaac ggactcctca acgattcata taataaaaga taacatgtta
     1621 ttgcatacat cacaaatgaa atgctaggaa aataaaagaa gaatagatca atgaaaaatg
     1681 aaaaagcaga acagttttct gctgataagc aggaactgtt ccgctgaatg tgtcttatct
     1741 atcgagaaaa aatcaatgga ttgatatcta agtaattcaa tacatataaa aagaggacga
     1801 tcgcattcac taaaaaggaa agaatgatga tcccatagcg aatggaaaca tatttaacaa
     1861 atacttttgt ttgaatacgg gcatatcttt ttcctttata gctgagcatc atgacaagaa
     1921 gcaaaagggt aatcacacct tgaaaaatca ttccaataaa cagaattcct gaggtttgat
     1981 aatttaattg gagaaaataa gctgctgccg caactaaatc gaataaaatc gcaataaatg
     2041 gcaaaataat cactcct
//
LOCUS       DADVXU010000197         1981 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.205, whole genome shotgun sequence.
ACCESSION   DADVXU010000197 DADVXU010000000
VERSION     DADVXU010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.205"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..425)
                     /locus_tag="KB953_002881"
     CDS             complement(<1..425)
                     /locus_tag="KB953_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HBB6867784.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
     gene            complement(534..1565)
                     /locus_tag="KB953_002882"
     CDS             complement(534..1565)
                     /locus_tag="KB953_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBB6867785.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          610 a          327 c          341 g          703 t
ORIGIN      
        1 ggaatctgtt ttgattttgt tccatttgaa atttctctca ctcgcttttt tgtctcatca
       61 tcaaataaaa tgaaagggtc ttctaaacga tcgataaata cttccattgt tgttgttggt
      121 agatgataag aagtcatgac aggcactgca ccaaggtaag acacagaaac agctagaagg
      181 tatgtgtcaa aagcggaact cttataaata ataactttgt ctccggattt cactccaaga
      241 tgagccaatt gataagcccg tttcaacaca ctttcgcagc attttttgta agttgtttca
      301 agtcctaatt ctgggaaagc tgaaagtggc tcatcaaaga taatcggggt ctcggaaaag
      361 ttagctgcag cttcttgaaa attcgtaaat aagttcaaag gggaatacgt tatggtctcc
      421 atcattcaca ctccattatc ttttgttaag taatatacaa attcaagata atcgtaacat
      481 aatcctttat ataataacat agtttattgt gaaaaaaaga tcatacattt gttttatatg
      541 gaagaaaccc gaacgaatgc cgctaattcg tgataagaaa aatcaaccat cttttcccag
      601 atatcttgaa aagaatggga tgagaggtat cgattaccac ataaataatt taccgcgtca
      661 gcatcatcta aatatagttc tgcataataa cgatatgttt ttctgcatag ttctggcgat
      721 acgtttttat atactgtaat tttgctcaaa atttggcgaa tagtccgagg agaaatttgc
      781 tgatcatgtg aggaacgaaa aacaaaagaa ttttcgtccg tattttgtgc tgttaactta
      841 aaatagttct ccagaaatgg catcgcttct tttggtattg gtactttatt cagtctgttt
      901 tcttttctat tcagattaag aatctgcttg gtcaaagata catctttacg tttaagttct
      961 actacttcat ggatctttaa tccgcaatac agaagaatgg cagtaattgc aaggttcctc
     1021 ggtctattct tttcttcaaa gctagcttct ctgcttgttt gaaagccttc aatcgggata
     1081 aacaaatgct caaaatcttt gaaaagttct gcatcgatat atcgcggagc tgctttggtt
     1141 gtttccggta aacttgtacc caaaagagaa atctcttctt caatcagtcc tttttgatac
     1201 aaaaatcgta aaaaatgatt taatgaatat tgcttccttg aaatcccgta atagctattt
     1261 tcaaacaatt gctccgtttc ttggttcgct gtagtttgga acgaacgagt atgtcgatag
     1321 attttagcta agaaattctg ataatcttgt ttttctaaat ggataatcag ctgatcatcc
     1381 acttcttcag aaaatacttt tctcaaataa tcagtaaaca gatgaaaatc ttttgcgtat
     1441 tcatagcgac tgcctaatga aagggaagaa cgattttctt ctaaaaactg ttgaaagacg
     1501 gaagggagca agtgtgtacg ttgctttaac ttttcttgca gttgttttct agttttgctg
     1561 tccattacaa attccctcca atagtgttca ttttctatat tttaccgcat ttcatcaaat
     1621 gaagatagac agtttgtctt gatcttagga agcaaatttt taactaagta ctagctaaaa
     1681 gatatgaagt aagaagatac atttttagtt attttattag tgcagtcatt cttttgatct
     1741 tttaattgat ttatcctctt tttagctatg tggttacttc atgagcaatg aaatggattt
     1801 ggttcgttct aatggcagga ggctctttct tttatttccg aaaaattaga aaacattttt
     1861 tcagaatcga ttggctctcc atttattcaa taatggtttg tctaacgata ttgttgggat
     1921 aattcttaaa gaagtattag aaatagttgg tacttcatta ctttacatga agatttattc
     1981 t
//
LOCUS       DADVXU010000198         1971 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.206, whole genome shotgun sequence.
ACCESSION   DADVXU010000198 DADVXU010000000
VERSION     DADVXU010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.206"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            89..571
                     /locus_tag="KB953_002883"
     CDS             89..571
                     /locus_tag="KB953_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867786.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSTFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            768..1658
                     /locus_tag="KB953_002884"
     CDS             768..1658
                     /locus_tag="KB953_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HBB6867787.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT          644 a          337 c          407 g          583 t
ORIGIN      
        1 tctctaaaaa aaaagttttt ccccctaatt tttttagata gaaaattgta tagtatgatg
       61 agagaaaaag tagtttgggg gaacgttaat gagaagaaaa gcagtaatcg atcggcttta
      121 tttatttttt tggttttttc ctatcgtttt gctacctttt acatgggtaa atgctgttgt
      181 acaaggtgcg gagatattag gcctgatcca aggaagcgga tttgtggttt ctaggaattt
      241 tccagctttg attgttttct atttagtgat aagtgtaggt ctcttattac caaaatctct
      301 gatcaatcac tacaattggc agctgcttta cttttcttgc ttattagtat ttacatctac
      361 gttttctttg ctgcctcgct tgattatcgg ccctaatggt ttgagcgaga gtggattgaa
      421 ggatttagct tattcctcca tgtatttctt gacgatcaaa ccatggttct atttagtaat
      481 tgcctcattt attcttgcaa cattttttta cttctggaca gaaatcagga taaaaagtga
      541 acgatctgta tctataaaaa ataactattg atggtggatc atcagtaaaa aaacatatga
      601 gctgcgtaaa atgcatgttt ctcatatgct ttttttatta ttatgtaaag aatgcgaaaa
      661 acgtttacat gatttttaca taacttaact atatttttac agcaaccata cgagggattt
      721 acagtcgact gataaacttt agttgtaaat aagaaaggag caataaaatg aaaaaagtat
      781 tgtttactct aggtatgatg ggaattcttt tagccggatg cggctcagga tcaggaaata
      841 cagatggctc tgccacacaa aataattcaa gtgactcaaa tgaacaagta aaaatcgttg
      901 cggtaggttc gacagcatta cagccgttag tcgatgcagc acaagaaagc tttgtacaag
      961 aaaatccaaa ttatcaaatc tctgtacaag gaggaggaag cggaactggt ttgagccaag
     1021 tcgaagctgg agcagttact atcggaaact ctgatgtatt tgcagaagaa agagacggag
     1081 ttgatgcttc aaaattagtg gatcataaag tagcagtggt cgggatggca ccaatcgtca
     1141 ataaagatac agatgtcaaa gatatcacca aacaggaatt gattgatatc tttacaggaa
     1201 aaatcactaa ctggaaggaa gttggcggga aagatcaaaa aatcaacgtc gtgaaccgtg
     1261 caaacggcag cggtacccgc gcgacattcg aaaaatgggg actggatggt gctacccctg
     1321 tgcagtccca agaacaagat tcatcaggaa cagttcggca gctggtaagt caaacaccag
     1381 gagcaatcag ctatttagct ttctcatatc tcgatgattc cacacaagca ctaagtatcg
     1441 acggtgtaga accaaaagaa gagaatgtag cagataacag ctggggaatc tggtcatatg
     1501 aacatatgta cacgaacgga aaaccttccc ctgaagtcca aaaattctta gactacatga
     1561 tgacagaaga aatccaagaa ggacctgtca aagaattggg ctatttacca atcacaatga
     1621 tggaagtaga acgtgatcac gaaggaaata tcaaataact agcagataaa catgccacgg
     1681 tagtcgttac tattaaggaa aaatcaacta tgtttcccca catatacatt tttcctttaa
     1741 ttgtgaaaaa tagttcctca tattttgccg ttttttgagc taataccgag ggtcagctga
     1801 ttgaacaccg tttattcgaa aaaaagagct acgacataga actgtcgtaa ctttttttgg
     1861 ctcttcgtca aatcgtgttg gtgaagacaa atttgaataa aacttagacc gatatgcggc
     1921 gcgttctgtg cgccgcatat ttttttagtt ttcaaagatc agaccttgtt g
//
LOCUS       DADVXU010000199         1925 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.207, whole genome shotgun sequence.
ACCESSION   DADVXU010000199 DADVXU010000000
VERSION     DADVXU010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.207"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(71..292)
                     /locus_tag="KB953_002885"
     CDS             complement(71..292)
                     /locus_tag="KB953_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867788.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(390..635)
                     /locus_tag="KB953_002886"
     CDS             complement(390..635)
                     /locus_tag="KB953_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867789.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            875..1489
                     /locus_tag="KB953_002887"
                     /pseudo
     CDS             875..1489
                     /locus_tag="KB953_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          663 a          326 c          287 g          649 t
ORIGIN      
        1 tttattgtaa tatgaagctg tattttacat atagtaacca aagagttaat aaaataaaaa
       61 gagcgaacca ttatagatca gctctttttt cattgctgtt tttaatctta tctagaatca
      121 cttcaaatgt ttcaaaggta tttacatcta actgacataa ccaaagcaag tacttctttt
      181 ctttgtaagt ccaatcaata ttacggtaag aggacattag attatggtac cttgctcttt
      241 gttcaactga agccaattcc cagcgttttt caaatatttt ttcttcttcc attattgatc
      301 cctcgcaaga cgctacctca aacttaaata atgggcagcg aaagtctaat tccttagatc
      361 taagtatagc aaacaaatat gaaaaaaact tagaaggtag taaaagttct tccgtctttt
      421 gctttgcttc tattcttaat gtgtttcgtt atcggtgccc tacaaagatt ttcattaatt
      481 acacactcat ttattgttgt gtattttctg acatttctct cttgatcaat tattttaatt
      541 ccatgggcat cctgatagtt cataggagag gagcggctat cagaaatagt tgcacctatc
      601 tcttgaactt cttttacaac ttctggaaaa tccatccatc ctgcttcagc tgtcaaaagt
      661 aataatctat aatatttatc tttccttttc aatctaagaa atcgtcaccc cattcttttt
      721 cttctattat aaactattta acttttagaa ttcaaaaacc gtttgtctat ttagagttta
      781 ccctaaatag acaaaaatat aacattgaaa aatttagagt tctaacgtat gattagggtg
      841 tattataaac gttatagaaa agtagggaat aaatttgaaa attgcttata ttagagtttc
      901 atccatcgat caaaatgaac aacgtcaaat tgaagaaatg aaaaagtttg gcgctgaaaa
      961 aatttttatc gaaaaacaat ctggagcgac gattagtcat cggccgattt tccaggaagc
     1021 tttagatttt gtcagagagc aagatatttt tattgtagaa gccattgatc gacttggacg
     1081 caattatgac gagattatcg attcagtgaa ttatctaaag aagaagaatg tgcgacttat
     1141 tatcaccagt ttaccgatca tggctgaagc aattggaaat cctctcctag ataaattcat
     1201 caaagactta atcatttaaa tctcagccat gattgctgaa caagaacgta ctgaatcgaa
     1261 acgacgccag gcacaaggaa ttaaaatcgc taaagcaaac ggcgtctata aaggtcgtcc
     1321 taagttatac agtgcagaca ccaaagatcc tcaacgtcgc cttgtctata ggagtatcgt
     1381 tcaggatctt gagaatgggg ttgctatctc aaagattgca acagactaca atgtgactag
     1441 gcaaacaatt taccgaataa aaaaagaaat cgaccaacta attgtatgat ttcttttttg
     1501 aatcacgtaa ttattaatta gctttgtcat caatacagac aatcatttta gattcatcta
     1561 gctaagttga ttacatttat gttaaaaaga atttagttaa cataaatata attgatgcat
     1621 aaatcgtcct tatttaaagg catttaacat tgacaactta gtataatgta tcttatgtaa
     1681 actaataaga tttgaatttt attttataca cactcgataa tagatcaatt ttatggtccc
     1741 ccgcatttac gattgcacaa gagaaacata aagtcgacct attagcattt cttttcaatt
     1801 caaacattaa aaaagtttat acctctgcta ctaaaaaatg aaattatcta tttaaaattt
     1861 aaaacggtaa atctgattca tccaaattgg aaaatggatc agcctgtatt ggagctttgt
     1921 atttc
//
LOCUS       DADVXU010000200         1892 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.208, whole genome shotgun sequence.
ACCESSION   DADVXU010000200 DADVXU010000000
VERSION     DADVXU010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.208"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..550
                     /locus_tag="KB953_002888"
     CDS             <1..550
                     /locus_tag="KB953_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867790.1"
                     /translation="KKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYG
                     IDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTL
                     LISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLS
                     EEKILLPENWAELRKLVEYGII"
     gene            537..>1892
                     /locus_tag="KB953_002889"
     CDS             537..>1892
                     /locus_tag="KB953_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HBB6867791.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTS
                     LL"
BASE COUNT          629 a          244 c          289 g          730 t
ORIGIN      
        1 aaaaaaaatt cccaaaatag atgaaaacag taaattgatg aaaaacatac aatcaatctt
       61 tgggatagaa gaagtttttg tcggagataa atgtatcttt tttgatcaac ctttattatc
      121 tgatggttat ggcatagatg aaacagatat tttcgctaaa gtaaatagta tattttctgg
      181 aaaaaaagaa actttaatta agcttcatcc tagagcaagg acaaatcgtt atggtgaaaa
      241 aaacatattg aattccagtt taccgtttga agtcttgcta cttgaaaatg acttctcttc
      301 aacacttttg attagtccta tatctacaat ttctttttca ccatctatga tgtttgaaaa
      361 gacgatcaac tctattatgt tggctaaatt aattttaaat gaatatacag cattatctga
      421 agataaaaaa gaaacattga caaatattgt tcttttctgt gaacattata accgtctatc
      481 agaagaaaaa attttactcc cagaaaactg ggcagaatta agaaagttgg tagagtatgg
      541 gattatttaa cacactttcc aagtcaaaat tagttaagtc cagtttctgg tatactatag
      601 ggaatttttt tattaaggga atttcattca taactattcc tatttttgta aatattacta
      661 ctcaagcaga atatggtttg attaataatt tcacttcatt tgtatcaatt ttttcgattc
      721 ttgttgggct aagcctaaat tcggcaatta ataatgcaag ttttgactat aaagatgaca
      781 taaatgaatt tatgtcatcg gtactttctc tagctacact ttcgttaatt gtgttcttat
      841 taatagggaa catatatttt ttgttttcaa atgctttttt tgatatgaac caacttgtat
      901 ttaatttgat gttattacaa agttttgcta gttttgtaat tagttatatc cagtcttatt
      961 ttacgattaa ctttaaccat tttaaatatt tgattttatc ttttatatct acttttttga
     1021 atattgcaat ctctgtagct ttgatgcaga cattatattt tgatgataaa tatgttggca
     1081 gagtagtcgg cggaacactg tcatttgtgt tgataggttt agtaatttat ttaagaataa
     1141 tgataaaggg tgctcatttt tttgataaaa aacagtggag atacgcttta aaaatctctt
     1201 tgccgctaat cccacattcc ttagcaaata tagttttagc acaattcgat agaattatga
     1261 ttaattctta tttaggtcct gctgcggggg gaatatattc ctacattagt aacataggta
     1321 ttattctaag cgtaatatgg atgtctacta ataacgcctg ggttccatgg ttctatggtg
     1381 agatgagtaa aaataatgaa aaaaaagtga agaaagttag taactattat ttagtagtct
     1441 ttactttgat tacgatgatt gtaatggtag tttcgattga tttagcaaaa tttatggctc
     1501 cgaaagacta ttattctggg ttatctttag tggttccgat aaccttaggc tatttctttc
     1561 agtttttata tagtttacca gttaatgctg aattttatga gaaaaaaaca tcgtttattg
     1621 cgattggtac agtagcaagt gctatagtta atatactatt aaacatgatt ttcataccta
     1681 ggttaggaat aattgctgct ggatacacaa ccgtatttac gtatttttta ctttttattt
     1741 ttcattataa tatcgctaaa aaaattacga ataaacaatt atttgacaca aaaaaaatat
     1801 gtatcataat aatttttgta tcaatttttt ctttgatact tcatatttta atcaatcaaa
     1861 tgttgattcg ttacgttttg acatcattat tg
//
LOCUS       DADVXU010000201         1863 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.209, whole genome shotgun sequence.
ACCESSION   DADVXU010000201 DADVXU010000000
VERSION     DADVXU010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.209"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            210..407
                     /locus_tag="KB953_002890"
     CDS             210..407
                     /locus_tag="KB953_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867792.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            513..995
                     /locus_tag="KB953_002891"
     CDS             513..995
                     /locus_tag="KB953_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867793.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1053..1649)
                     /locus_tag="KB953_002892"
     CDS             complement(1053..1649)
                     /locus_tag="KB953_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBB6867794.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
BASE COUNT          583 a          276 c          370 g          634 t
ORIGIN      
        1 gaaataatta gtttttaagg aatatgtttt aattttttga aaaaaattct tctaaataca
       61 acagctgttt taattgtgaa aacgaaaaca gtacgctata atgcctgtaa ggttgatttt
      121 taactgtaaa taaaaatcaa tgagttaaat ctccaaagaa atagcagtgg taatgtggag
      181 aaaagttcca gcagtttagg aggattttga tgacggaaga aatgattaat ttaggagaac
      241 aatatgcatg caaaccaatt ggttttacaa aaacggtcat tggtgaagtt gtatcaaaaa
      301 tgacaaactg cgccgtggtc aaagttgccc aatgtgctgc agaggaccaa gaattattag
      361 atgaaaaagc aagcatggtt gttgcaaaat atgatacatt tgaataaaga aaacaattgg
      421 acgtgcggcg tgctgcaagt ccaatttctt tgagaaggta aatcagtatt cttttcctat
      481 tttctggaga agaaaaagcg aggcgagttg tcatggtaac attctttacg gttctattgt
      541 ttttattcgt tgttgtgatg tttttatttc tttggctggt tcgaaaagaa attatttatc
      601 ggactgtgcg aaatcgatgg gtctacttgg tgataccctt cttagtgtca ctagtcattt
      661 ggtatacact catcagtcat cctacagcag atgaattggc aaaagggatc ttgtcagcaa
      721 tgatttttat tagttttttg ttggacagta gaggaatcac agaagaagga cttgttttga
      781 atagctttga caaaaaaggt gttccttttt ctgagatcaa taaaattgta ttatatcagc
      841 caaaaggttc taaaatcgta aaaatgaatt ttttccgaaa cggatggcga ggaccgatgc
      901 tgaaattttc tgcatcatta gaagaattag ttccattttt gtcccaacgc ttaaacgatg
      961 aggcagagat tgatattatg attgatccag aataaaaaca gaggttgcga caagatgttt
     1021 gtcacaacct ctgtttttta tttcaaataa aattatccaa atacgcggaa gcgatcggca
     1081 tcgttcatgt attctttttc ctcagccggt gtttctggtg aaccgaatac taattgcgaa
     1141 cgtagtttcc agtttgaagg gatattccat tcttttgcaa ctgcttcgtc aataactggg
     1201 ttgtaatgtt gcaagtttgc gcccaatcct ttatcaacta atgctaccca agtattagct
     1261 gttgcgattc cgtttgattg ttctgaccaa tctgggaagt tgtctgcata taattcaaat
     1321 tgttcttgta gaccttttac cacgtcagta tctttgaaga ataatactgt accataacca
     1381 tttttgaaac cagctaattt gttttgtgta tttgggaaag cttctgctgg agtcaatggg
     1441 cgaagtgctt cttcagtgat ttcccaaagt ttttcatgtg catctccgaa aaggataact
     1501 gcgcgtgttg attgagcatt gaaagcagtt gggctttctt taattgcttc tttgatcaaa
     1561 gttgttaatt cttcattaga aagtgaaaca ttacgtccta gatgatagat tgaacggcgg
     1621 tttttcaaag tgtcagtaaa agttgtcata ataaaatttc tcctttgatt tgcgttttgc
     1681 atttttttaa ttgattacga aatgaagtat agcaccttac aaaaagtaag taaacagata
     1741 tgctcacaaa aaataagcga agttcaaaaa acaattttaa taagaaaaat cgttatgtaa
     1801 ggattttctt agttatcgct ttcattgttg tgttttagga atttttgctt atttgaaaaa
     1861 aac
//
LOCUS       DADVXU010000202         1811 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.210, whole genome shotgun sequence.
ACCESSION   DADVXU010000202 DADVXU010000000
VERSION     DADVXU010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.210"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            287..694
                     /locus_tag="KB953_002893"
     CDS             287..694
                     /locus_tag="KB953_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867795.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="KB953_002894"
     CDS             708..1109
                     /locus_tag="KB953_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867796.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..1482
                     /locus_tag="KB953_002895"
     CDS             1111..1482
                     /locus_tag="KB953_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867797.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1518..>1811)
                     /locus_tag="KB953_002896"
     CDS             complement(1518..>1811)
                     /locus_tag="KB953_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867798.1"
                     /translation="FNETQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINLYYS
                     NKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT          676 a          236 c          307 g          592 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1381 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1441 atgaatcgag aagcagaaaa aataataaac aaactcacct aatgaatttc aagcgagttt
     1501 gtttgttatt tttttattta atctttgtat ctttctggat caacgaaagt atactttata
     1561 tagtcataac gccgatgatc gctacgtgcg tccggcacgt cagtcacgat atcaaacaaa
     1621 aagtatacac ccttcttcat tctagttttc gcagcaggaa ttttgaaata gttcttatta
     1681 gaatagtaga gattgattaa taagctatct tcgattgcta aaaagaaaac ttctgaattc
     1741 catactttat aaaaatcttt gataaatcta ttcgaagggt caaatttaaa ccataattgt
     1801 gtctcattaa a
//
LOCUS       DADVXU010000203         1760 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.211, whole genome shotgun sequence.
ACCESSION   DADVXU010000203 DADVXU010000000
VERSION     DADVXU010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.211"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..506)
                     /locus_tag="KB953_002897"
     CDS             complement(<1..506)
                     /locus_tag="KB953_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBB6867799.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSM"
     gene            complement(914..1618)
                     /locus_tag="KB953_002898"
     CDS             complement(914..1618)
                     /locus_tag="KB953_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867800.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT          506 a          325 c          322 g          607 t
ORIGIN      
        1 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
       61 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      121 ttagcgatta gtttacgtga ttgattttcg tacagttcca tgagaatcgc ctgcttcagt
      181 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtccgtcca
      241 caatcatggc agagaaaacg ggtacgtttt aattctaaaa tagtcaactt ttctttgact
      301 ttcaacatct gagtgcgtgt cgtataggaa ccgttgcgga taatacgaat cgatccacag
      361 tgttcacaat gggaaggtga gtcatccaat ctccctttaa taaactgcgc ttgaacgcct
      421 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      481 agtaattttc tagtatgatt atccattgag agactccttt ggtagggtga cgttttggtc
      541 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa aagtgttagt
      601 agaaaatcgc tttcgatttt ccaccaacac aaaatattat agaaccgctt ttttctctat
      661 cagaaaatat gtttattatt agaatttttt atagttaagg ttaggcatct taagaaatca
      721 ggcaactcca agatatcttc aggtagaaga gaagtcgcct aacattgtag gatcataggt
      781 gatttgttgg acaggtcaag ctgatatttg agcctgatgt tagaaggaat ttctttttac
      841 ttgctgtaga aattattata tgactaaaaa ggctgggatt ttaaatccca gccttttatg
      901 acaaatagaa tttttatcga tcagtaatcg ctgtcgctgc aaattgttcg atcaaaggaa
      961 tcaaagtcaa tttgactttg tatttccgtt cgagtgcttt ttcgataacg aactcatttt
     1021 cagtagtcat agaaatagtt gctaatttac gaggttcatc agcatttttt tcattaataa
     1081 ctacttcatg ttcattttgt tctgtgtaaa atttaggata ctcagcaacg gcttttgaaa
     1141 aatcagcaaa acttaaagcg gcattcgtat cagttgagcg tgtattttca gttgtgtccg
     1201 cttttggttt ttccgttttt tcttctggca cactgactgt ttcttctgta actggtgtcg
     1261 caacagcttg cgcagttggt tcatcgtttt tcaatcgttg atcattccag taagacacga
     1321 tttcaataag aagattgtca cggaatcgtt cgaaaatccc gttttctcct gaacgaattc
     1381 tctttaatac agtattgaag ttgtcatcga ttggcacgtt gatccgtgta tcttcatttg
     1441 ttttcccagc taatccgatc gttaagattt gacggttttt gggatcaagc atgacttgta
     1501 acggcaaacc ataacgtcgc aagctctctt cttctagaga acggaataat tgtcctctta
     1561 caccatccaa tgaaggacgg aattgattta ataatttttg tttctgtttt ttcttcatat
     1621 gttctctcct tcgctcatac cctttcgatt ataagaaaaa aacagccaaa tgaaaaggag
     1681 taatgcaaaa aaacttgcgt atacgcaaat tacaacagaa aaatttgcgt attttcccgt
     1741 tttttgaaag aaaaataaaa
//
LOCUS       DADVXU010000204         1741 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.212, whole genome shotgun sequence.
ACCESSION   DADVXU010000204 DADVXU010000000
VERSION     DADVXU010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.212"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            49..1572
                     /locus_tag="KB953_002899"
     CDS             49..1572
                     /locus_tag="KB953_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HBB6867801.1"
                     /translation="MDPTVFEAEEHIQKVIHKMFRPDNAYTKFDFRNGRPTKSAILTT
                     SSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTMEDPDFPRIAITYSLEENSRD
                     SSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNRLARKRAEFKQFGKQIDLVIV
                     VDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTYPEKTKGLIVTFRKPATMEKN
                     VEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKEVSSMESIDEHTPLEIRIEYV
                     KAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEEQIGVYETVKGSLMEEEPEKE
                     GEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSDAREEIEKALAKLNKTEGVKE
                     VYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHAFSNEWFVSEKELHASNLQYM
                     PGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT          628 a          279 c          352 g          482 t
ORIGIN      
        1 atcaatcgta tgatcgatca aatggagtct gtcaaaaaag aaagttattt ggaccctact
       61 gtttttgaag cagaggaaca tatccaaaaa gtcattcata aaatgttccg tccagacaat
      121 gcctatacaa aatttgattt tcgaaatggc cgtccaacga agtctgcgat cttaaccacg
      181 agttccattg atatggccaa aaagtattat cgggccatca aagagatgac gaaagaatct
      241 gattggttaa taaaagagtt tcccaatcag cctatccgtg aaggacgaac gatggaggat
      301 cctgatttcc cacgaatcgc tattacctat tcattagaag aaaatagccg agattcaagt
      361 gcccaacaag aagaaatgca aaaaattatt gaggaatata atcagtatta tgatactgcc
      421 tggtcactag cagacattga gcgttataac ggagacatca ataatcgttt ggcacgaaaa
      481 agagcggaat tcaaacaatt tgggaaacaa atcgatttag taatcgtagt ggatcgtcta
      541 ttgacaggtt ttgacgcccc tacgattcaa accctatttg tggatcgtaa tctagagtat
      601 gcaggattga tccaagcctt ttcacgtacg aaccgtacgt atcctgaaaa aacaaaagga
      661 ctaattgtta ctttccgaaa gccagcgaca atggagaaga atgtcgagga tgcgacaaaa
      721 ctgtattcag aagctaaaga agaatcgggt ttgatttatc caagttacca agaatccaaa
      781 aaaagattca agcaagcata tggtaagtta aaagaggttt catccatgga atccattgat
      841 gaacatacgc cacttgaaat ccgtatcgag tacgtcaaag catttcaaga attgaatcgt
      901 gcgtatgaat cgttagtgac ttatgatgat tacaatgatg acatggtgca atcttcaact
      961 ttgaacaatc aaattcaact tttggaagaa caaattggcg tgtacgagac cgtgaagggt
     1021 tcattgatgg aggaagaacc agaaaaagag ggagaagatt tttcaacgat cgaattttat
     1081 agtgaaaata ccactaagtt gtatgatatt gattctactt atattgatca attactagga
     1141 acttatgctg cgaatagttc agatgcacga gaagaaattg aaaaagcttt ggctaaattg
     1201 aataaaacag aaggagtcaa agaagtttac cgacaaattt taaatgcatt tgatgtgggg
     1261 actttagata gtaatgaaga tatctttgtc attaaacgtc gctattttac acaagcaagt
     1321 gataatttga ttcatgcctt ttccaatgag tggtttgttt ctgagaaaga attacatgct
     1381 tctaatctgc aatatatgcc aggtgaagat ccaattccta atatgaaagc aattatcaac
     1441 agtaaggatt acgaaggata taaagccaaa catccagaag cgaaaccatt taaataccca
     1501 caagaaatga agcgagcatg gcgtaaaatg ttagatgatg agttgattcc acttgaaaat
     1561 gaattgaggt aaacgagaag agttaagaaa attgtacgat tcaaagatag tagtaaagtg
     1621 gaagtgtaga aaatagggat agatgtcttt ttacttgttc acattttata acggagtatt
     1681 taagaaagct attgaataat gattcttcca tctttttcat tcatttaccg gttctgttgc
     1741 a
//
LOCUS       DADVXU010000205         1730 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.213, whole genome shotgun sequence.
ACCESSION   DADVXU010000205 DADVXU010000000
VERSION     DADVXU010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.213"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..901)
                     /locus_tag="KB953_002900"
     CDS             complement(<1..901)
                     /locus_tag="KB953_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HBB6867802.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYF"
     gene            complement(1060..1326)
                     /locus_tag="KB953_002901"
     CDS             complement(1060..1326)
                     /locus_tag="KB953_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6867803.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1316..1672)
                     /locus_tag="KB953_002902"
     CDS             complement(1316..1672)
                     /locus_tag="KB953_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBB6867804.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          502 a          330 c          207 g          691 t
ORIGIN      
        1 aaaaatacct tttaaatttc ttatagtcct ctgaaatcaa aatatagcgt gatggatact
       61 catcaaaaga agaatcgtat tcttccttta aaattcccga tttacttaat ttttctagta
      121 catcaaatac tctatcactc aagtaactcc ttaaattgtt atcactttcc cattgaacta
      181 cttttctata agttgaatta tcagttgaaa agatagaatt ttcatccata aacgcatata
      241 ttagcagcaa tctttcatca ttgtcaaatt ctattggatc aaaaatatct aagttgtcat
      301 caaaatcttt atcagttccg gtaacttttt tgttaattac tgaaggtgtc ttgtaagaaa
      361 tagaatctaa atcaaagcgt tttgattcta aattattttg tgctttctca attaaataat
      421 tcactttttt tgtgagaaac cttctgatat tgtctttgct atatgttcca taaaaaccct
      481 tatcttctaa agagtccctg tacttcttaa cttctatata ctcttcagcc aaaacttgtg
      541 gaactggttc atctaagaaa aaaaccccta cctcgccttt gctattttta taaaagtaat
      601 caatttctgc tatagttccc gatctataat tatcaactgg agaacccaac ttatggccaa
      661 aaatcgctaa taatatatca ctattttcta caatttgttt attaataaca ctttgcccag
      721 agctattatt tatataagta ggaatcgcat tcgtttccca tcgaattggc atcaaaatta
      781 cttttgtatt atctgtattt tctatattcc atctatatat ggtttcttct acttcattcc
      841 tctttgctgc tatatcacct ggtgatgcta taaatacttt tattagattt atggttttca
      901 tttattgtac ctttctcttt atgtctcttt aaagtaattt ttgtataaaa tttattgact
      961 tttacactat aggacaactt ttatctattt agtacaaaaa ggcccactaa actaattgat
     1021 tagttaggag cctattacag gtatattttc aactcgcttt taagcaaatt ctactaccag
     1081 ctttttgcct aatgccctag cgatatcatc aagtttatcg aaagatacgt tatcccctct
     1141 ctcaattcga gcaatagtag attgagttgt atgtgctttt tgtgcaagct ccgcttgagt
     1201 taggcctgct tcctcccgtg catgataaag agcaaccgca gtttcgagtt ttcttccttc
     1261 acggtcatat gcttctgcaa aatctttgtc tttttgcttt tcttcaatca attttttaat
     1321 catcatttgt gaaccactcc tctctaagtt cttttgcatg tttaagttca cttattggtg
     1381 ttttctccgt ttttttagta aatccatggg taatgacata ccttccattg accacatgga
     1441 aatagatagc tctttggatg tttgatccta ccttacttct taattcgtac aaattagtgt
     1501 ctaatttttt cacccatttc aaccgttgag ctactagcat cccattcttt tctgtttctt
     1561 ctattgtacg taaaagcttc gcactatctt tttttggtaa tgtttccaac cattctaaaa
     1621 attcatcatg accattaggt cttttaaatg tttcaaaaga tattttttcc atacccaatt
     1681 atatcatata ttgattttat attatagtat ataacataaa aattcgaatt
//
LOCUS       DADVXU010000206         1690 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.214, whole genome shotgun sequence.
ACCESSION   DADVXU010000206 DADVXU010000000
VERSION     DADVXU010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.214"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..1406
                     /locus_tag="KB953_002903"
     CDS             <1..1406
                     /locus_tag="KB953_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBB6867805.1"
                     /translation="VEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTNTP
                     GNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTN
                     LPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADK
                     YNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPD
                     GSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWK
                     EKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKT
                     EPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVV
                     SSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLIL
                     LGTLGLLGYKNRKKENE"
     gene            1540..>1690
                     /locus_tag="KB953_002904"
     CDS             1540..>1690
                     /locus_tag="KB953_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBB6867806.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIK"
BASE COUNT          666 a          252 c          364 g          408 t
ORIGIN      
        1 cagtagaagg cgaaaaagta gaagttgggg gcacagtaga tctaacagat aatgttacta
       61 acttgccagc gttaccagaa ggaacaacag taacagatgt tactcccggc ggtacaattg
      121 ataccaacac accaggtaat tacgaaggtg tcattgaagt aacgtatcca gacggtacga
      181 aagatacggt gaaagttcca gtagaagtga cagacaatcg ttctgacgcc gataaatatg
      241 aaccaacagt agaaggcgaa aaagtagaaa ttggaggcac agtagattta acagataatg
      301 ttactaactt gccagcgtta ccagaaggaa caacagtaac agatgttact cccggcggta
      361 caattgatac caacacacca ggcaattatg aaggtgtcat tgaagtaacg tatccagacg
      421 gtacgaaaga tacggtgaaa gttccagtag aagtgacaga caatcgttct aatgcagata
      481 aatacaatcc tgaaggacaa aaagtgacta cagacttaaa taaagagcca gatgcatctg
      541 agggaattaa gaacaaagaa gatttaccaa aaggaactac ttatgcttgg aaagagaaag
      601 tagatgttag cacagctgga aataaaaaag gtactattgt agtgacatat ccagatggat
      661 catctgatga agttgaagta gacgttacag tgacagatac tcgttccgat gcagataaat
      721 ataatcctga aggacaaaaa gtgactacag acttaaataa agagccagat gcatctgagg
      781 gaattaagaa caaagaagat ctaccaaaag gaactactta tacttggaaa gaaaaagtag
      841 atgttagtac agctggaaac aaaaaaggta ctgtagtagt aacatatcca gatggatcta
      901 aagaagaagt agaagttact atttctgtag tagataaaaa agcaccgaat aaacctcaag
      961 ttgatcctat tacagagggt gaccaaattg ttactggtaa aactgaacca aatgcagagg
     1021 tgacagttac attaccagac ggaagtcaac accatgggac agctgataaa aatggtaatt
     1081 ttacagttaa agttcctaaa ttagaggcag ggacaaaagt gatagtaact gcaactgatg
     1141 aatctggtaa tactagtgaa cctactaatg tagttgtttc ttccaacaaa aaagacagtg
     1201 gaaaaaacgg tagtaaaggt agtaagactg acaatcaagg tagcaacagc aatcaagaca
     1261 aaaatagagg caaaagccag tcctcaaaag tttttcctaa gacgggagag agtgacagta
     1321 atatctttac tattagcggt ggtttaatac tgttaggaac gttaggatta ttagggtata
     1381 aaaatcgaaa aaaagagaat gaatagaaaa tcaatttctt aagtcttctc tgagaagagg
     1441 aagaatcttt ttcttcctct ttttgctttt ttatgatggg aaataagtgt tagttaaaga
     1501 tgaatatact ttttatagaa aggaaaagaa tgtaattaag tggaaaaaat agtaattatt
     1561 ggttcaaacc attcagggct agccgcagct cgatttctaa acttaagcga ttcaaattat
     1621 gaagttattc taatcgataa aaaatcagaa attagttact tggaatgtgg gacatcgtta
     1681 tttataaaag
//
LOCUS       DADVXU010000207         1666 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.215, whole genome shotgun sequence.
ACCESSION   DADVXU010000207 DADVXU010000000
VERSION     DADVXU010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.215"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            214..894
                     /locus_tag="KB953_002905"
                     /pseudo
     CDS             214..894
                     /locus_tag="KB953_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            956..1573
                     /locus_tag="KB953_002906"
     CDS             956..1573
                     /locus_tag="KB953_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867807.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
BASE COUNT          605 a          246 c          335 g          480 t
ORIGIN      
        1 attgaaatag gacataaata tatgagtatg agaaaggtga ttccatgttt aataaatggg
       61 caaaccaccc aatgaactcg agaggtagag ataatttgaa ggagacggac aagttctggg
      121 aagaagtata tagtaaaaac ggttctgttg caaagttttc caaaaaatct attttagtgt
      181 aaaattgaga aaaaagacag agaggacaga gtaatgaatc attttaaagg caaacaattc
      241 aaaaaagacg tcattattgt cgctgttggt tactacctgc gttacaatct aagctatcgt
      301 gaagttcagg aattgttata tgatcgtgga ataaatgttt gtcatactac gatttatcgt
      361 tgggtataag agtacagcaa agtcctctat tatctttgga agaagaaaaa tagacaatcc
      421 ttctattcat ggaaaatgga cgaaacctat atcaaaatta agggacgttg gcattatctt
      481 tatcgtgcaa ttgatgcgga cggcttaacc ttagatatct ggttacgaaa gaaacgggat
      541 acgcaagcag cctatgcttt cttaaaacga ctccataaac agtttggtga gccgaaagca
      601 attgtgaccg ataaagcacc ttctcttggc tccgccttta gaaagttaca gagtgtgggt
      661 ttatatacta agacagagca ccgaactgtg aagtatctta acaatttaat agaacaagac
      721 catcgaccta ttaaacgacg aaataaattt tatcaaagtc tccgtacagc ctcttccacg
      781 attaagggca tggagaccat tcgaggaata tataaaaaga accgaagaaa tggaacgctc
      841 ttcggctttt cggtgtctac tgaaatcaag gtattaatgg gaataacagc ctaagatatt
      901 tggagttcag agagggcgcg tttgattttc aaacttcgca acagaaccga gaaccttggg
      961 ttttcaagtg ctttttggtt cattaatctt aaccatagtg tttttaaagt ggaaaacaag
     1021 caacaaaaaa agtatgtgga gccttgtagg aatagttctt attattttta ctgtgactgc
     1081 agcaacagaa gcaaattatt cgcataaaat tcagtcgtat aaaggtcgtt tagttatgac
     1141 caaaaaagat gttacacgtt atacatcctc aagaactgga ggcctatatg actatgcacc
     1201 attagatgca ttgaaatata aagatcattt aaagaaacat gaagtaaaag ttggtgatcg
     1261 gtgggagaat gcatctcata aagcatataa ttccaaaata gtctatacag tagaagatgc
     1321 cgatgagcaa aatatcacat tacctgtatt caattattgg ggaacagttg tgaaagtaaa
     1381 tgggaaaaag agcactatag aaaatgacaa tggtttagtg tctattaaag gtacggggaa
     1441 aagaactata gttgaggttt catctacgta tccaactacg atgattctat ctttattaat
     1501 tagttcaata acttttatat tcttatttac acgttattta ataagatgct gcggtgaaat
     1561 gaaaatagat tagatagata ttataaatat gttgattcta ttttcatagc aatggagaaa
     1621 atacattagc aatatataaa agaaggtatc aagagtcaca gtgaat
//
LOCUS       DADVXU010000208         1664 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.216, whole genome shotgun sequence.
ACCESSION   DADVXU010000208 DADVXU010000000
VERSION     DADVXU010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.216"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            131..505
                     /locus_tag="KB953_002907"
     CDS             131..505
                     /locus_tag="KB953_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBB6867808.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="KB953_002908"
     CDS             complement(619..1248)
                     /locus_tag="KB953_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBB6867809.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="KB953_002909"
     tRNA            complement(1382..1454)
                     /locus_tag="KB953_002909"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DADVXU010000209         1602 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.217, whole genome shotgun sequence.
ACCESSION   DADVXU010000209 DADVXU010000000
VERSION     DADVXU010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.217"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..413
                     /locus_tag="KB953_002910"
     CDS             <1..413
                     /locus_tag="KB953_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HBB6867810.1"
                     /translation="SPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIE
                     NEVTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILD
                     IDSEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            418..1365
                     /locus_tag="KB953_002911"
     CDS             418..1365
                     /locus_tag="KB953_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HBB6867811.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          538 a          224 c          321 g          519 t
ORIGIN      
        1 cttcaccttt acgtgaagtt tctaatcttg aagaattaat agatgaacat ctaaaaaatt
       61 tagataaaga cgtctacttt tcagttgttt ctgcaagacg taatccctat tttaatatgg
      121 taaaaagaat tgaaaatgaa gttactatag ttaataaatc aagatacaca tctaggcaac
      181 agacacctga agtttttgaa atgaatgcct caatgtattc ttttaaacct gaatttctaa
      241 aaaaaagtaa ttatattttt gatgggcaat gtgggataat aaaaatgaga gattatctaa
      301 ttttagatat tgatagtgaa gaagatttta aatggttaga atttttgttt cctaaatttt
      361 tagaagattc ttcggagctt caagatatct ataattattt atatcggagg taaaaggatg
      421 agctgttatc ttatcaccgg tggggctggc ttcattggtt ctaccttagc caatcattta
      481 ggagaagaac atgtggtaat cgtcgtcgat gacttatcaa tgggaaaagt agaaaatctt
      541 gatatgacga aaaatatcac ttttattgag ggagatgtcg ctgagaaatc cttgatggaa
      601 gagatcatga aagctaataa atttgattat atctttcatt tagcagcagt tgctagtgtg
      661 gcagattcag ttgcccgacc agtagaaaca catcgtgtga attttgaaag tgtattaatg
      721 attttagaat tgatccgcaa gtatcaacct gatttaaaac ggatcgtgtt tagttcttca
      781 gcggctgttt atggggatga accgacttta ccaaaaaaag aagagtccgt gattcgccca
      841 ttgacgcctt atgcggttga taaattcgct gcagaacaat atgtgctaga ttattgtcat
      901 ttatatgatg tacccggtag cgcagttcgt tttttcaatg tctatggtcc aaatcaaaat
      961 ccgaattctc catacagtgg tgtgatttct attctcgtag atcgctataa gaaacaaatc
     1021 gtaggagaag caacttcatt tacattattt ggtgatggaa gccagtctag agactttgtt
     1081 tatatagatg atgtaatcca agcattactt ttagttgcga atgaggaagc tgcactgggg
     1141 aaacaattca atgttggaac gggtaaagca accacacttt tggaattgat tcatgcaatc
     1201 aatgaaattc tcggtgtaga attgacactt gaatacaaag aagagcgttc gggtgatatc
     1261 catgactctc tagcagatat tactaagatc cagtcaattg gctatcaacc gaaatatggt
     1321 attttaagtg gaatgactaa ttatttgaaa actgaaatca attaaaacat actaagataa
     1381 gattagaggg taaattcagg ctctttgtca ataaggactg atgagttttg taaaatcaaa
     1441 tctgggatag aagggctctt tgtcaaatga tgttggtaaa gagaaatctt agaataattt
     1501 ggacagaaca gaaagaagaa aacctctttc tgttctgttt atttggttaa aaatgttttg
     1561 ccatgacgaa tcataattcg tagtttgaaa ttgtaaaagt tg
//
LOCUS       DADVXU010000210         1601 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.218, whole genome shotgun sequence.
ACCESSION   DADVXU010000210 DADVXU010000000
VERSION     DADVXU010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.218"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..233
                     /locus_tag="KB953_002912"
     CDS             <1..233
                     /locus_tag="KB953_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBB6867812.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
     gene            392..>1601
                     /locus_tag="KB953_002913"
     CDS             392..>1601
                     /locus_tag="KB953_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBB6867813.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVL"
BASE COUNT          518 a          309 c          295 g          479 t
ORIGIN      
        1 tattaaaaaa tcttcctgaa acacttgatg cacaacttcg cacgaaactt caaaaccttt
       61 taacgtatga agaaggtatt tacaatgcta tgatttaccc gtattctaat ggcaaaatag
      121 aggccaagat tccccatatc aagacactga aacgattatc ttatggattt aaatcatttg
      181 agaacatgaa aatcaggatt tttttgatca atcaattgat tcaagtgaaa taacaaaaaa
      241 atctgagaca gaaggtcagt ccttctatcg gctctatact ttttgatgtt ggtgaacttc
      301 accaacatct tttttgtata aaaaaatata gaaaagacac cctgaaatcc tttagaatta
      361 atttggctac caattcatag gaggattcaa gatgtctcat actcagctta tcaaagatac
      421 cctaaatatt ctagacctaa atatctattt cgaggaaaat tgtttaacta aagaaaaata
      481 caaagggcaa atttgcttga tttacaaagg aactcttctg tacaagccag aagagtgttc
      541 ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg gggacgacaa ccgttcggtt
      601 gttactgaat gatgtgtctg agtatcgcac ttatctggag ctgaagaaac aacgatttaa
      661 atgtaagtct tgccaaagaa cattcgtagc ggatacctct gttgctgaaa aacactgttt
      721 cattagtcaa aaagttcgtt ggtctgtcat tgctcgctta aaagagaata cttccatgac
      781 ggaaatcgct cgtcagaaaa atatttccac ttcttcggtc tatcgtgtca tgaagcgatt
      841 ctaccgccca ttgaatcctt tcaaacagac tttgccaaag gtcttatgct ttgatgaatt
      901 caaatcggtt cgaagtgttg ccagtgcaat gagttttatc atgatggacg gacaaagcca
      961 tgcattattg gatattgtcg aaaatcgacg cttgccttat ttagaacgct atttttctcg
     1021 gttttcatta gcgactcgtg aagccgttca attgattgtt attgatatgt atgcgcctta
     1081 tgtttcgttg gtgaaaaagc ttttcccgaa tgctcagttg atcatcgatc gtttccacat
     1141 cgtccaacat attggcagaa ctttcttgaa tcatcgagtg aaagaaacca cggtacgact
     1201 taaaggaaat tcccactctc aacgagggtt agggaaaaaa ttgaagcgct attggaaact
     1261 cctacaaaaa gaagaagcaa agttaaacta tgaaaaacgg gtttggcgtg ccagttttaa
     1321 agattatttg actgaaagtg agattgccga tcgactgtta gctgcttgtc ccaacttacg
     1381 ccaaggctat cagttatacc aagacattct ttatgcggta aagaaaagag atcagtcact
     1441 atttgaggat tgtttaacta gagacattag cggtctgcca gaaacctata caaccacttt
     1501 acgaacgttt aaaaagtttc tgccacagat ccaaaatgcg cttcactaca gctattcaaa
     1561 cggaccattg gaatgcttaa acaaccacat caaagtatta a
//
LOCUS       DADVXU010000211         1546 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.219, whole genome shotgun sequence.
ACCESSION   DADVXU010000211 DADVXU010000000
VERSION     DADVXU010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.219"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(62..280)
                     /locus_tag="KB953_002914"
                     /pseudo
     CDS             complement(62..280)
                     /locus_tag="KB953_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(546..740)
                     /locus_tag="KB953_002915"
     CDS             complement(546..740)
                     /locus_tag="KB953_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867814.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(840..926)
                     /locus_tag="KB953_002916"
                     /pseudo
     CDS             complement(840..926)
                     /locus_tag="KB953_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          545 a          236 c          207 g          558 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattata ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt agtacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaattatgt actacctaaa taaagtagtt tataattagc aagtatatat
     1321 atttaaaaat ttaagattaa tttttttatg acttgtacaa atttatctga tattctctta
     1381 ttaagaacta cctaatttgg gtagttctta ttttttacat tttaagtagt atttaattgc
     1441 aatttggtag tattttttta ttatcttatt cttagaacgg tagtacttaa tataaatata
     1501 ggtagtatct aatttaagta ttaatggtag tatctaaatt aacagg
//
LOCUS       DADVXU010000212         1544 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.220, whole genome shotgun sequence.
ACCESSION   DADVXU010000212 DADVXU010000000
VERSION     DADVXU010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.220"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            47..1216
                     /locus_tag="KB953_002917"
     CDS             47..1216
                     /locus_tag="KB953_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HBB6867815.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT          554 a          274 c          249 g          467 t
ORIGIN      
        1 agctttattc tttctgtaaa aaagaaagga gactaaataa actattatga aaaaaggaaa
       61 acatgctaaa aaaagaaaat attcattttt aatgatcgtt gtcgtgttgt ttagccttag
      121 tgcgttctct acaggaacta ttcttggatg gcaattttac aataatgaac actctcagat
      181 agttgcccat gctcaatcag ctcaaaaaaa gttgatactg actaccaatg aacaacagct
      241 cgttgaaaaa atcgatcaac tattaagaaa aaatgattat attggctcaa tttacgtaaa
      301 gaaaaataat cgcatcatca tggaaaaagg ctatggctat gcgaataaac tcacgcaaca
      361 accaaatgat ccttctcttt attatcagat tggttcggtt caaaaagcat tgactactct
      421 tttgatttta aaacaagtcg agcgtgggca cctttcttta gatactaagc tggctcaatt
      481 ctatccacaa attgctggca gccagcaaat tactatccgt gacttgctgt atatgcgctc
      541 aggcttgcac cgaactgctt ctccaaccat accgatgact gatgaagaag tcgttcaatt
      601 tgcgattcag cacttaaaat ttacagatta tcagacctct cattatgatt cattgaactt
      661 taccatattg actggaattt tgatcaaatt aacaaatcaa ccgtatgaaa aattattgac
      721 cgatgatatc atcgaacctt taggattaca gcatacaaaa ttttatgacc aagtcaaaaa
      781 ttcatccaat catgcactat cctatcaaat gtctaaagag gatgattact ttaaaccact
      841 gacagaatct gaaacagaga tccataacga attagggact ggtaatatca gtatgtccgt
      901 ctatgattta aatacgctgt ttacacaagt tttatctgga aaactagttt ccaaagactt
      961 actcttctca ttatggcgtg aaaatgcgaa tggacatcca tatagtggtg gggtatatag
     1021 cggagataat tatatcctgt cgcaaggaaa tatcgactct tttgatacat ctgctatatt
     1081 tagcaaagat tcacaggatg ctatcatcat ggaaagcaat ataaaagctg acaaaactat
     1141 caaactgcct actgctgatc taagaaacca aatctatgac ctgatggaag gaactactaa
     1201 gttggcaaat agctgatatg atgaaaagat agcaaatact acttctttga atagcatttg
     1261 cttaaaaaaa gtactcgact aaacgtagga aagagtgtgg gacaaatcta aaaatcaatt
     1321 tttgctacag actttaattc caaataaaca gaacagaaag aggtgttctt ctttctgttc
     1381 tgtccaaatt attctaagat ttctctttac caacattatt tgacaaaggg cctattaaat
     1441 ctaaaatagt atctttagat tgctaaaata tgtgctagca aatcattcat ggcttgttct
     1501 tatttttaat tttatgcaat aaaaaagaag aacaattagc gtga
//
LOCUS       DADVXU010000213         1544 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.221, whole genome shotgun sequence.
ACCESSION   DADVXU010000213 DADVXU010000000
VERSION     DADVXU010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.221"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            34..1500
                     /locus_tag="KB953_002918"
     CDS             34..1500
                     /locus_tag="KB953_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBB6867816.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          426 a          290 c          326 g          502 t
ORIGIN      
        1 tttctgaaaa tttaaagaat tggggtatag attatggaaa aacagcaaga caagggcttc
       61 ttcgggcagc cgaagggatt gtcaactttg ttcttcactg agatgtggga acgatttagt
      121 tattacggta tgcgtgcatt acttatctat tatatgtacg atacagttgc caacggggga
      181 cttggattac cacgagcaac agcattagct atcatgtcga tctacggatc tttgatctac
      241 atgtctagta tcgtcggcgg atgggtatct gaccgagtgt taggagcaaa acgcactgta
      301 ttcttcggtg gagttttcat tatgcttggc cacatcgtct tggctttacc atttggtgta
      361 agtacattgt ttgtttcgat ggcattgatt attatcggaa ccgccttttt gaaaccaaat
      421 gtttctggca tggtcggaca tctttattca aagactgact tgcgaagaga tgcaggattt
      481 tcgatttttt acatgggtgt caacttaggg gcattattag caccactaat cgttggtaca
      541 atcggtcaag aatataacta tcatttaggg ttctctttag ctgctattgg gatgttcttt
      601 ggattattac aatacatcat ccaaggacgc aagacattag acggagtggg tacaacaccg
      661 ccgaatcctt tgacaactga agaacgtaaa ggattcatcc gtaacttgat catcgcactt
      721 gttgttatcg ctgcagtatt cggcggggca caactaactg gtcatctgac aattgacttt
      781 tttgtcaata caatcagtgt attggggatt atcttaccgt tgtattactt tatcaaaatg
      841 ctgacttcaa aagatgtaac tgctgaagaa aaaccaagag tatgggctta tatcccatta
      901 ttccttgcag caatcatttt ctggtcatta caagaacaag gttcatctat cttagcttta
      961 tttgctagtg aacgtacaca atctacgcta tttggtgtaa cgatccaagc aagctggtat
     1021 caatcattga atccaatttt catctttatt ctgacaccag tattcgtaac gatgtggacg
     1081 aaacttggtg aacgtcaacc atctacagta gtgaaattct ctcttggact tttatttgca
     1141 ggattatcgt acttgctatt gatgttgccg ggtatgcttt atggtacaag tggaaaagta
     1201 agtccattgt ggttgttggg aagtttcttc atcgtggtta ttgctgaatt gtgtctttca
     1261 ccagttggtt tgtctgtgac aacaaaatta gcaccacgag catttgaatc acaaacaatc
     1321 gctatttggt tcttagctga tgcttcttca caagcaatca atgcacaaat tgcacgcttc
     1381 tacacaccgg gaacagaatc tgcttacttc ggtattatcg gtgcagtagc aatcgtcggt
     1441 ggtatcttgc tatttactgt gaaagaacca atcaaaaaac taatgggctc tattcattaa
     1501 tcaatgaaca taaaaacagg aattgcttat ttgcagcagt tcct
//
LOCUS       DADVXU010000214         1513 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.222, whole genome shotgun sequence.
ACCESSION   DADVXU010000214 DADVXU010000000
VERSION     DADVXU010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.222"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            34..456
                     /locus_tag="KB953_002919"
     CDS             34..456
                     /locus_tag="KB953_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867817.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            466..669
                     /locus_tag="KB953_002920"
     CDS             466..669
                     /locus_tag="KB953_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867818.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            880..1464
                     /locus_tag="KB953_002921"
     CDS             880..1464
                     /locus_tag="KB953_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBB6867819.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          534 a          219 c          283 g          477 t
ORIGIN      
        1 gttttcttat ttttgatttc gaggtgatag acaatgaaaa ataagtttat tttatcttta
       61 ggtattttaa ctattggaat tagtgtaact ggaactacaa ttcctacatt tgctgatagt
      121 acaaatgaac caactactgt agtagataat aaccaagaat taactccttt agataggaac
      181 ggaatagcca ttggtaaccc aactaacgac ttaactgtta ctgaaaatta tttaatagtt
      241 tctccattac ttgctcgagc agcttctaaa acgatacgag tgacaggaac gatgattttt
      301 gataaagttg gttggccttc tagacctcct caatcacgga attggaaaga aaaaagaaat
      361 ggttattggt actatggaaa attgtattta caaagttata aatcagctgg aaacaattgg
      421 attgcgactt attcaggaac cttaactaga aagtagggat tttttatgcc agaatttgtg
      481 aacacaccaa atttttatca agatagcgat attctaagta aaattaattt accgaaagaa
      541 gaatcagctt tacaagttca gcgaacttta caggagacac aaaaaattca aaaatcaatt
      601 tctaatctag aagattctga agaaaaaaga aaaaaaagag accaaaaaac atatcaaaaa
      661 gaaaaataat gattttaaaa aaagagtgtc tagtcgacat tctttttttc taattgaaag
      721 tgttcataaa atcacccatt tctggacact ttcttttttt gctttatttt tcaaaaaaac
      781 aattcatttc atgttcataa acccataaca atatctatgt tcagttaaac tatatggata
      841 aagttatgtt acaataaaat taatgaagga gtgatttttt tgaaaatagg atacgcccgg
      901 gtatctactg gtttacaaaa tttggattta caagaagaca gcctaaataa atttggttgt
      961 gaaaaaattt ttactgatca tatgagtggg gcaaaaagta atcgaccagg attagaaatg
     1021 gccattgatt ttgttcgctc tggcgataca cttgtcgttt ggcgcttaga tcgtttaggt
     1081 agaaatatgg aagatttgat ttcaatcgtc aatcgactga atgagcgtgg tgtgagtttt
     1141 catagtattc aagaaaatat tacgatggac aaatcaagtt ctacaggaca gctcatgttt
     1201 catttgtttg cggcatttgc agaatttgag cgtaatttga tcctggaacg atccgcagca
     1261 ggacgagcag ctgctcgtgc aagaggacgg tttggtggtc gtcctgaaaa attgtctgaa
     1321 caagatttgg aattactgaa gactttagtg gatagcggta cacctattaa gactattgcg
     1381 gaacggtgga acgtctcaag aaccaccatt tatcgttatt tggcaaaaat ggaggagaaa
     1441 aatggaaaga caaattctca ttagtttttg tcatttaatg ctgaaaattt ttgccattta
     1501 tctagaaaag tga
//
LOCUS       DADVXU010000215         1480 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.223, whole genome shotgun sequence.
ACCESSION   DADVXU010000215 DADVXU010000000
VERSION     DADVXU010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.223"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            64..972
                     /locus_tag="KB953_002922"
     CDS             64..972
                     /locus_tag="KB953_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HBB6867820.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..>1480
                     /locus_tag="KB953_002923"
     CDS             972..>1480
                     /locus_tag="KB953_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867821.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGV"
BASE COUNT          529 a          197 c          316 g          438 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taaaaacact gaagttatgg aagctacaat tgtcgaaaat
      181 ggtcttccat atgatttatc agaatgtaag gtatattttg aatcagttgt tggcggaaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat caaatatatt
      301 tttgataaat attccatgca gtgtttgcat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcatcaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaattg aagatttaat tgctgatatg
      481 acagatttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgatc gctggagaga ggaacaggag aatagtttcc aagattggag agaaggacaa
      601 gaatcagatt acttgtcatg gtttgaatca atcaaggata ttttgaagtc tatcgatcca
      661 ggtggagtaa tgttagccga attaatggat gcacgtgtag atcttcaagg tgtacgccat
      721 gaatcgattt ctgaacgttt cctatccgat ctaaattatt tgtatcaaaa aatgaaagca
      781 gcacttttca cgattgaata cggtgaaatt gaagtgactg atattcttca agacgatctc
      841 ttttcagata atcacgaagt cgaaaaaatt gaaacagtag aatttccaat tgaagaagga
      901 gctttgatta ttgcaactgt tgatgatcca aaacagaatg ttttcactct tgagaaagtt
      961 ggggtgattt gatggttaaa gtaaagagaa tgatggaaac tgatgaatct ggtgttaaac
     1021 gtcaatttat gccaattaca catgtctctg cagttttagg cttagaaaaa atgatatcag
     1081 gacaatcaaa agttctttcg gttaatggta aatatggtgc agtcatttta acgaaagctg
     1141 atttaggatt agagaatgca attactgaac taccttatgc aagcgagaca agcgatggta
     1201 ttcttactgc tgaaatgttt caaaagatcg taaatggaga aggtggaaca tatattttgc
     1261 cgattgctac tcctgaacaa ttaggaggta taaaagttgg ggaactctta gaagttactg
     1321 aagaaggtgt tttatctgca actaagcaaa cagacttcaa tttctctgaa gaattaaaag
     1381 gaaaattaga atctctaaaa atattaaaag caggtgcaaa tatatcaatt gcagaagatg
     1441 gaacaattag ttctacaggt ggtagtggga ctggtggtgt
//
LOCUS       DADVXU010000216         1467 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.224, whole genome shotgun sequence.
ACCESSION   DADVXU010000216 DADVXU010000000
VERSION     DADVXU010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.224"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..626)
                     /locus_tag="KB953_002924"
     CDS             complement(<1..626)
                     /locus_tag="KB953_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBB6867822.1"
                     /translation="MDNHTRKLLVLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLRFPR"
     gene            914..>1467
                     /locus_tag="KB953_002925"
     CDS             914..>1467
                     /locus_tag="KB953_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBB6867823.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPY"
BASE COUNT          508 a          214 c          280 g          465 t
ORIGIN      
        1 tttctaggaa agcgcaggaa gtattgagtt agtttgtaaa gtctgcgatc ttcaaggatc
       61 gtaaatagct gatgggttgt cccatcaaca caaataaaac tcatggaacc agaaacagac
      121 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
      181 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      241 ttagcgatta gtttacgtga ttgattttcg tacagttcta tgagaatcgc ctgcttcagt
      301 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtctgccca
      361 caatcatagc atagaaaacg ggtacgtttt aattctaaaa tagtcaactt ttctttgact
      421 ttcaatatct gagtacgtgt cgtataggaa ccgttgcgga taatacgaat cgatccacag
      481 tgttcacaat gggaaggtga gtcatccaat ctccctttaa taaactgcgc ttgaacgcct
      541 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatacg
      601 agtaattttc tagtatgatt atccatagag cgactccttt ggtagggtga tgttttggtc
      661 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa agtgttagta
      721 gaaaatcgcc ttcgattttc caacaacaca aaatattata gaacccttta ttctaaagga
      781 tttcaaggtg tctgtttgca tttattagaa aaaagaaaaa gtattggcaa cagaaggtgt
      841 caccaacact agatattata gagccataat atgctgtata atatctagtg tataggctta
      901 agtggaggta tatatgaaaa tattaactgt tgaagatgat aatatgataa gagagggaat
      961 aagtgaatat ctttcagaat ttggatatac tgttattcaa gctaaagatg gaagagaagc
     1021 cttgtcaaaa tttaatagcg atataaattt ggttatctta gacattcaga taccttttat
     1081 aaatggttta gaagtgttga aagaaattag aaagaaaagc aatttaccaa ttctaatctt
     1141 gactgcattt agtgatgaag aatataaaat tgatgcattt actaatttag tagacggata
     1201 tgtagaaaag ccattttcat tgccggtctt aaaagctaga atagattctt taattaagaa
     1261 gaattatgga catttagaga agtttgaata taaggatcta tcggttaatt ttaatagcta
     1321 tacagctaaa ataaatggtg aagaaataga tgtaaatgca aaagaactag aagtattaaa
     1381 atgtttattg gataataatg gacaagtgtt aacaagaatg caaattattg attatgtatg
     1441 gaaagatagc gaagaaactc cttatga
//
LOCUS       DADVXU010000217         1456 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.225, whole genome shotgun sequence.
ACCESSION   DADVXU010000217 DADVXU010000000
VERSION     DADVXU010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.225"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            321..1361
                     /locus_tag="KB953_002926"
     CDS             321..1361
                     /locus_tag="KB953_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBB6867824.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
BASE COUNT          573 a          193 c          228 g          462 t
ORIGIN      
        1 atggtagtat gttttatatg cataaggtag ttcttaattt attttttgtt ttgacactgg
       61 tagtacttaa ttatctttaa ttttaaaact aaattttatt catacgtttt tctggtagta
      121 tttaattata aataatattt atttaaagta gttcttaatg actttaaagg tagtacataa
      181 ttcattttat ttctgatttt aggtagtatt taattaaaaa acagttttaa tgcgtttgca
      241 ttttttattt atctttgcta ttttcgaaaa gagtaggtaa ttttacataa ataaaacaac
      301 gtgaaagtag aggatctaca atgactgatt ttaaattttt taaggcggat agagtttaca
      361 acgaattatt ttatcaattt ccaaaagtct ttattgtttc tgacgaatac aaaaaaatga
      421 aagattcaac taagattgcc tatatgcttt taaaagcaag attagagatc gcaatcagca
      481 aacggcaaat cgatgaagaa ggtaatgttt attttactta tacgacaaat gaactatgta
      541 gagtattaaa ctgccaaaaa caaaaagcga tagcaatcaa aaaagagttg gaatcctttg
      601 gtttattatt acaaaagcag atgggattta acaaacagtt agggaaaaat aatcctaata
      661 gactatatct agcagaatta aaagtctcag aaaatgatat ctacttactc gaaaaatttg
      721 atagagagaa tagggaaaac gttgataaat cagagggtat gaaaatcata cccaccctcg
      781 acgaaaaatc agacgctgaa tcccttgggg ctcaagaggg tatgaaaatc ataccgtgcc
      841 aaaacgttga taaatcagag ggtatgaaaa tcataccaga acttaataat aatatattag
      901 acactaatag acacaatata gacactgaaa aagaccgcct acaagatcaa ttgttgttag
      961 acaattttga gacaattatg acaaacgaca gcattgctac gtttgtccct gaacgagtat
     1021 taaatttgat aaaaacattt tcttcaagtt acagtgaagc tcaaaaaacc gtccagacta
     1081 ttcataatgc aaagaaaaaa gctgaaatag aaagtgatat ttcgatagtt tttgaagaac
     1141 tcgatagtta ttatgtcaat gcagaacaag aattatacac gacactgtta aaagcctatc
     1201 aaaaattaaa aaccgaaaaa gtcgaaaata tccagaacct gatttttgtc tatgtaaaaa
     1261 attggtttat cgaaaaacca atagctgcta aagtatcaag tgaaaaacgt ttgaattatg
     1321 caagctcccc aagcactatt acgaaagact ggttagagtg aaactttact gacaaagtga
     1381 ttgttgcttc atggagtaac attaaaatta aaaatttttt attattctaa ttatattacg
     1441 aatattacaa aataat
//
LOCUS       DADVXU010000218         1425 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.226, whole genome shotgun sequence.
ACCESSION   DADVXU010000218 DADVXU010000000
VERSION     DADVXU010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.226"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(45..1184)
                     /locus_tag="KB953_002927"
     CDS             complement(45..1184)
                     /locus_tag="KB953_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HBB6867825.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT          367 a          305 c          260 g          493 t
ORIGIN      
        1 aataatgcta atctggctct gttttttcat ttattaaaaa aatcttacgc taagtaggat
       61 aaaataattc cttgatattt ttcgatcata tccaaataat tatcgatttc aacatactca
      121 tctacttgat gaggcaaagt aacgacacca ggcccaaaga cgacaaagtc gaaggaatgg
      181 ttagctttcg taaattcggc tgcatccgtt gttgctgccg ctccaactaa aggtaatggc
      241 tggctgaatt gttgctgaat gcaatgaatc aaaggagaat ctggatcagc tttgacaggt
      301 attttgttgt aatcgatcat caattccaaa tgatagtctg tctcttgatt caattcattg
      361 acgattgatt gcagcaatgc aatgatttta tcatttgggt attctggaat cgatcgaatg
      421 ttcccttgga gctgcgcatg gcttgggatg ctgttgactt gattcccgcc actgatcaat
      481 gtaacattat ggattgtttt tcctaggaca ggattctcga ttgtttcagc gaggtgattc
      541 atttcagcat ttgctttcgt aatgaattca tttaggtgat tgattgcatt ataaccttgg
      601 tctggcatag agctatgcgc ttcttttccg tgcgaggtga ctgtatagtt gattgatccc
      661 atgtgcgtat acatcaaact gtaattggtt ggctctccga tgatcaaggc atctaagtca
      721 tctacatagc ctgctttcgt gagctgttcc ccacctagtt cacccacttc ttctccgaca
      781 gtggctaata atttgactgt tccgttaaat ggttttcctg actctttcag ttcgatcatg
      841 gcaatcacca ttgctgccaa gccactcttc atatctgtag caccacgtcc gtacaaacgg
      901 ttcccatgga tttctgctgc aaagggtgca taggtccaag acgattcatc accagccgcc
      961 acgacatcca tatgcccaga taggcctaac acttttcctg attggccctt ttggtaacga
     1021 gcaatcaaat tatctctacc atctctgtaa gaaacaatct ctcctgttat gtcatgcttt
     1081 gccaaaagct tattcaaata ggctgctact tcaccttcgt ttccattcac tgatttaatt
     1141 cggatgatct cttgtaaaat cgcgattttt tcttcttttt tcatctaatc ctctcccttg
     1201 ccatttcttt ttattgtatt tgagtgtgct cgaattttca aataggagca accaaagtaa
     1261 aaatgaagaa agagagccac tttatgaaaa atatgctgct acttccctcc tattttattt
     1321 atacgagagg acttgctcat agattcctat tctactgttc tatccttaaa agctcttata
     1381 tgatgctagt tattgttagc tttccttttt caaggatgat ctttt
//
LOCUS       DADVXU010000219         1405 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.227, whole genome shotgun sequence.
ACCESSION   DADVXU010000219 DADVXU010000000
VERSION     DADVXU010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.227"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(149..448)
                     /locus_tag="KB953_002928"
     CDS             complement(149..448)
                     /locus_tag="KB953_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBB6867826.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            799..>1405
                     /locus_tag="KB953_002929"
     CDS             799..>1405
                     /locus_tag="KB953_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBB6867827.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFAR"
BASE COUNT          474 a          225 c          200 g          506 t
ORIGIN      
        1 tgttaattaa aggataaaat acaatataaa gaaacatctg tttctttatt tagaataaaa
       61 aaatacagac tctgttcaaa taaatgaacc tagcctgcat tatgagattg actatacttt
      121 ttaattcatt gagatgaata actcgttttt ataaatgccc aaaagtaatc ggtgccgaag
      181 ccattcctcg acctaattct ttttcaaatt ttcctattgt tgatatctcc atataaaacg
      241 gtgatactgg tttagacaat tctcccctga ttgctaacgg acgtaaacta cgttccaaat
      301 tatttatgac tttaggataa tactgacctt tttctaagcc tgtctttgcg tctatcaaaa
      361 gtatacgttc cttttctttg atctctggat ttaaaacgag atcatataat tttagtacca
      421 tgttttctat ttcttcactt ttagacatca cgatttcctc ccaaaaatat atttcttccc
      481 actacattct ataaacaaag tatacgctac atctagattt ttttaaatca ttactggatt
      541 gttaaaacct ttcaatacac tgttatggca acgattttta ttttaaaaac attctaaatt
      601 aaatcagtaa aaatttttat tctaagattt ttattagaat tacgcctaat ttgtgtctct
      661 cagggcaatt ctaagaacgt ttcaagagat agcgacacta tcgttatcga aaaaggcgaa
      721 aacctatctc attttctgct aatgtaagtt ggtttagctt ttgttaatat gactttatga
      781 aaatggaggt aattaaatat gatttttcct aaagttaatg aagcaagctt gaataaagga
      841 tccttttttc atatctatga aaacgaagat tttgaactgt ataatatcct ttcacaaaaa
      901 gaggtcatcg aaaaccaaat aatctgccat ccaattattt acaaatctat tttcaagtat
      961 gcattttttg cttccaatga ttttcaaaga atggagatta ccgactgtat attcgaaaac
     1021 tgtgattttt caggctgtat attgacaaaa agcatgttgc atcgcgtgaa atttgtcaat
     1081 tgtaaattaa caggtactaa attgatggaa agttatattg ggaacacgtt attcgagaat
     1141 tgtaaaatgg actatgtaaa tttatctggt tcaaacataa aagaaagtac ctttgagcac
     1201 tctgtcttat caagcgcaga ttttgtagac tgttccctca ctaaaataat gttttctact
     1261 aatgatttag aaaatgctag cttttttgat actgatttaa aaaatatcga tttaagtagc
     1321 aactcctttg aaatgattga agtacgctcg gaacatctaa aaaattgtac gcttaatcag
     1381 ttgcaagcac ttggttttgc tagaa
//
LOCUS       DADVXU010000220         1364 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.228, whole genome shotgun sequence.
ACCESSION   DADVXU010000220 DADVXU010000000
VERSION     DADVXU010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.228"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            82..>1364
                     /locus_tag="KB953_002930"
     CDS             82..>1364
                     /locus_tag="KB953_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HBB6867828.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          465 a          262 c          257 g          380 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taat
//
LOCUS       DADVXU010000221         1355 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.229, whole genome shotgun sequence.
ACCESSION   DADVXU010000221 DADVXU010000000
VERSION     DADVXU010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.229"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..1355
                     /locus_tag="KB953_002931"
     rRNA            <1..1355
                     /locus_tag="KB953_002931"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DADVXU010000222         1337 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.230, whole genome shotgun sequence.
ACCESSION   DADVXU010000222 DADVXU010000000
VERSION     DADVXU010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.230"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(76..375)
                     /locus_tag="KB953_002932"
     CDS             complement(76..375)
                     /locus_tag="KB953_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867829.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(381..611)
                     /locus_tag="KB953_002933"
     CDS             complement(381..611)
                     /locus_tag="KB953_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867830.1"
                     /translation="MMEEFIRKNISDEYADFYEQSNEKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            complement(861..1061)
                     /locus_tag="KB953_002934"
     CDS             complement(861..1061)
                     /locus_tag="KB953_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBB6867831.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
BASE COUNT          462 a          222 c          200 g          453 t
ORIGIN      
        1 ctaacttaaa aaatatctcc tttcataagt atggtgataa aatccgttcc gggctacctt
       61 tttaggtagc ctactttaat ctttgtatct ttctggatca acgaaagtat actttatata
      121 gtcataacgc cgatgatcgc ttcgagcgtc tagcacatca gtcacgatat caaacaaaaa
      181 atatacgtct ttcttcattc tagttttcgc agcagggatt ttaaagtagt tcttattaga
      241 atagtagaga ttaattaata agctatcttc gattgctaaa aagaaaactt ctgaatccca
      301 caccttataa aaatctttga caaatctatt cgaaggatca aatttaaacc ataattgcgt
      361 ctcattaaaa agcataacca ttactccgat ctatttttaa acttagtctc tacctctaat
      421 atatatcgag tttttatttt gccttcagag aatactgttt ctttttttgc agttacaggt
      481 tgtttatttt cggaaaaagc taatatagct aaaattgaaa catccatctg gaatttgtct
      541 ttttcgttgc tttgctcata aaaatctgca tattcatcac taatattttt tctaataaat
      601 tcttccatca taaagatcac ctcgaaaaga gtatacgaac aaacgttctt tttgtaaagg
      661 tggaatttat tatactaaat aaataggtga acaagtactt gtgccaaatt atgtgccaaa
      721 aaaaatcgaa tttaataaaa cacaaacaaa aagaaatcct attatgataa catttcttat
      781 aataacaaac acaacagaag acgtgtaata gttagtcagg aacgtacaaa taacccctgt
      841 atcctttgcg gtacaggggg ttattttgta taagtagaca ttgtagtttc taaactgcta
      901 tgtcctaaac gtttcgatac gctaagtata tttactcctt gataaagtaa aatagatgca
      961 tgcgtatgtc ttagtccatc aataaacagt cgaagagaaa atagggaaaa attgattact
     1021 aaagaaaaac ttactaggtc gacggtgtag ttgattttca tacttcaata gattcgaatt
     1081 aaacgatttc gattggctta taccagtcct atccaacgaa agatatatcg ttcttaactc
     1141 tagcggtata acttgaagct ttacgtaaag ccaatgggaa tgaactggag atgacttttt
     1201 attattttta gtaatagctg atgggttaat acttacaact ttccttttca aagtaccata
     1261 taaaaaaacg gtggggttgt ttagcgaaat aaaggatcta agccaatagc ttagatcctt
     1321 tattctatgc tttatat
//
LOCUS       DADVXU010000223         1313 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.231, whole genome shotgun sequence.
ACCESSION   DADVXU010000223 DADVXU010000000
VERSION     DADVXU010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.231"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            23..1291
                     /locus_tag="KB953_002935"
     CDS             23..1291
                     /locus_tag="KB953_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HBB6867832.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVRKGILFLPIVKRMKEKNKV"
BASE COUNT          324 a          236 c          318 g          435 t
ORIGIN      
        1 ttttattgag gaggaatttt ttatggaaaa acaattaccg agtccagtac gcattttagt
       61 gtacatgctg aagtggctgg tgatcacagc gattctcggt atattcatgg ggagcttatc
      121 tgcttttttc ttaaatagcc tgacagttgt gacagatatc cgactatcac atccttggct
      181 cctttatcta ctaccagtta gtggtgcgct ttttgcttat ctctatgcgt atcacggcgg
      241 tctttctagt cgtggaaaca acttagtgat cgatcaagga aatggtggag aagaaaaaat
      301 cccgttgcga cttattccac ttacactgtt tggaaccata actacccatt tatttggagg
      361 atctgttggc cgtgaaggaa cagcagtcca aatgggggga gcgttagcag ataatacagc
      421 acgtttgttc cgattggata aatcagaacg ggaaatcttg attatcagtg gaatcagtgc
      481 gggttttagt tctgtatttg gcacaccttt agctggaaca ttatttggat tagaagtgtt
      541 ggcaatcggg aaagtccgaa cagaagcgat cttccctagc ttctttgctg cactgtttgc
      601 aaattttatg acagaaagct atggtgtttc tcatctacat taccaaatgg gtgtgattcc
      661 tgaatggagt atgttgctgt tcttcaaagt ttttgttgct gggattgctt ttggtttgat
      721 tggttgggta ttcagccgtt cgatcgtctt tttaaaagcg cgatatgcac aatggattcc
      781 tcatcctgtt tggcgaaata cgataggtgg ggctgtagtt gtagccgcag cattgatttt
      841 gcaaacacaa cgttatctcg gattgagtct tccattactg caggaatcat ttaatggaca
      901 ggcacatgtc tatgatttta ttgggaagtt attcttcaca gttttgtctt taggcgcggg
      961 atatcaaggg ggagaagtga caccattgtt tgaaattggt gcaacgcttg gtgcagcact
     1021 agcgcctttg ctccatgttt ctgttccttt tcttgcagga ttgggcttta tcggagtatt
     1081 ttctggtgcg acaaatacac cgattgcctg ctttgtgatg gggatcgaat tgttcggttc
     1141 acaagcggca gtatacttct ttatgatctg cttgatcagc tttatgtgtt caggcaacag
     1201 cggtatttat tctgctcagc aagtagcggt cagaaaaggg atactctttt tgccaatagt
     1261 taagagaatg aaagaaaaaa acaaggtgtg acatatgttt tgtcacacct tgt
//
LOCUS       DADVXU010000224         1307 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.232, whole genome shotgun sequence.
ACCESSION   DADVXU010000224 DADVXU010000000
VERSION     DADVXU010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.232"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..636)
                     /locus_tag="KB953_002936"
     CDS             complement(<1..636)
                     /locus_tag="KB953_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBB6867833.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRY"
     gene            complement(636..>1307)
                     /locus_tag="KB953_002937"
     CDS             complement(636..>1307)
                     /locus_tag="KB953_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBB6867834.1"
                     /translation="IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQ
                     YLPDWHPWEDYRNFFVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDID
                     YSDSFTLIIEHETGIVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETS
                     SMTDIQLYDEVMHDNNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLIN
                     DIEVN"
BASE COUNT          444 a          222 c          146 g          495 t
ORIGIN      
        1 gtatctcaat atatatgttg ctaaatcatt actgttaatt tcaagattcg aatatttacc
       61 aaactgtttt tttactgatt tagggaaacc aaataggttt ataatataat ccaattcatg
      121 tatacagtct aattctactc ctccccccat agatttaaag gcagaataat tatttctata
      181 atcagtacct tttctccaat caggaagata agtagaacaa atagctctta cagaatatac
      241 ttttttactt tgaatactat tacgaacaac ttccatgata tttttatacc tcagtggagc
      301 agcaacataa atatttttgt aatcataatt atattgagta agatcaatac tctcagaaaa
      361 agcaggtttt tctaaaaata tgtaatcaac tttttcaaca attacattta gtgtttccag
      421 atgcttaaaa gtcggatttg taataaaagc tatatcataa tgtgtatcca aatcgtctct
      481 attttgaata tacgttattt cttcaaactc tttttttcca ctgctactat aagctgttat
      541 atcaaaatta atagaattct tttttccata gcttattaga ttttttagat gtctctttcc
      601 aattgaccct aacccaaaaa aaattacttt catcattaat ttacctcaat gtcgttaatt
      661 aagttaataa tatacttatc tttttcaaaa gtatattggg gttccatttt cttttccaaa
      721 agcgtgaaaa attctttaat ctcctctaaa tatgcatctt caataatagt atgtgaataa
      781 ttattatcat gcataacctc atcgtacaat tgaatatctg tcatactaga agtttcttca
      841 ttccatgaaa atagagaatc cgggttacct tcccattcta ttaaaatctt ttcaccaatt
      901 atagttaaat ttcttttagc tactcttgaa acaacatcta cacttaaagt tccaactata
      961 ccagtttcat gctcaataat aagagtgaaa ctgtccgaat aatcaatatc aagattggat
     1021 aatttctggg tcattacagt aaacgattta actttcccaa atgtatctat aatccaaggt
     1081 aattcaatag cgaatatttc tctacaccca ttagtttttt tgtttgaaac aaaaaaattg
     1141 cgataatctt cccaaggatg ccagtcgggt aaatactgtc ctacatggta ttgataagaa
     1201 atttttttat tatgcgtcac tttagactta atatattgaa tttcttttct cttcaaaaat
     1261 gtagaagata gataaagtaa tctatctttc tttttagcta cttcgat
//
LOCUS       DADVXU010000225         1305 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.233, whole genome shotgun sequence.
ACCESSION   DADVXU010000225 DADVXU010000000
VERSION     DADVXU010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.233"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..155
                     /locus_tag="KB953_002938"
     CDS             <1..155
                     /locus_tag="KB953_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBB6867835.1"
                     /translation="KDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDAI
                     SYGVRK"
     gene            174..>1305
                     /locus_tag="KB953_002939"
     CDS             174..>1305
                     /locus_tag="KB953_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBB6867836.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSL"
BASE COUNT          544 a          110 c          225 g          426 t
ORIGIN      
        1 ataaggataa gtgtattaat tgtggaaaat gtaaaaaagt ttgccaaatg aataaagatt
       61 cagttgagaa ttgcaatcat ctggagtgta tcagatgtgg acgatgtaaa aatgcttgtc
      121 cagtggatgc aattagttat ggagtaagaa aatagagagg agtagttagg aaagtgataa
      181 aaaatgctat agcatatatt acaagaaaga aaaatagaac tttaataatt tttatcatat
      241 taactatagt tctttcttgc ttgtattctt gtttaacaat aatgaaatca agcaatgaaa
      301 tagaaaaggc tttatatgaa agttctaatt cttcaatatc aataacaaaa aaagatggta
      361 aatattttaa tgttaatcaa tttaaagata ttgaaaaatt aaaagaaatt gaagaaaaaa
      421 taattcaata tgatggatta gcaaaactaa aagatgctaa agtagttagc ggagaacaaa
      481 gaataaatag agaagattta tctgacgaat ttaagaatgt tgtttcactc gaagctacaa
      541 ataatactaa aagaaatatt ttatttagta gtggagtatt tacaattaaa gaaggtaaaa
      601 atatagaaga aaatgataag aattcaatta ttgttcatga agaatttgct aaacaaaaca
      661 atctaaaatt aggtgatgaa gttgatcttg aattactaga tattgaaaaa agtggaaaaa
      721 taaaaagtca taaatttaaa attataggga tcttttctgg taaaaaacag gaaacatata
      781 caggattatc ttctgatttt agtgaaaata tggtgtttgt agattattca acaagccaag
      841 agatattaaa taattcagaa aataatgaaa ttgcaaataa aattttaatg tattctggta
      901 gtgcagaatc tacagactta gccttaaaca aattgaaaga gcttaaaatt gatgagtcaa
      961 agtattttgt tgaaaaagat tctaatgcgt ttgaagagtc tttagagtca gtgagtggaa
     1021 taaaatatat aataaaagta atgacttatt ctattatgtt aggtgggatg gttgttcttt
     1081 cattaatctt gattctatgg ttaagggaaa gaatttatga aataggtata ttcttatcta
     1141 ttggaacatc taagatacaa attataatgc aatttatatt cgagttaata ttcatatcga
     1201 taccaagtat aatatcttcc ttatttttag gaaatgtatt actaaaagta attgtagatg
     1261 gattcattaa ctcagaggat tcaatgattt ccggtggaag tttaa
//
LOCUS       DADVXU010000226         1292 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.234, whole genome shotgun sequence.
ACCESSION   DADVXU010000226 DADVXU010000000
VERSION     DADVXU010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.234"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(262..876)
                     /locus_tag="KB953_002940"
     CDS             complement(262..876)
                     /locus_tag="KB953_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBB6867837.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT          417 a          258 c          214 g          403 t
ORIGIN      
        1 cttgtctctt tttccactac tttttatttt tctgacacta catattctta taactcattt
       61 ctcataactg tgaaacaact tataaaaata acaaacttta aacaaaacaa gcttaaaatc
      121 aaagatgatc catcttaaaa taactaaaac aaaaaaagta gtcgttgtta atcctcatta
      181 ataatcgttt tctttttttc aaacgctcca tttttatgtg gaacattatt tttttaagga
      241 actttgtcaa catgtggata actattttcc acttgtgtcc tggactagct gttttgctgt
      301 aaaatacaaa acttgctgca gcaaaaagat cgttgcaaga gaggctgttc cgcagatcgc
      361 tggatctaga tttggcaaag tcaagactgc atcatgtaaa ttggcgtggt tgtccaaaac
      421 caaatcagca aattcaaata atcgcaaacc actgctatgt tgcgatttga tacttcgtga
      481 caaatcaaat ccagtaacaa tgattaatgg atgaccattt tctttgatcc actgagctag
      541 ttcgatgatt gccgggtttc ttccttcatt cgaaagaaga agaaaaatgt cttctgattt
      601 gacagaaagc tgatcaataa ttgcctgtcc tgcaccttct agcgtttcat agacacctcc
      661 ggctggattt tcaatcaata tacttggtac aagatccggg gtttgatccc aaaattcaaa
      721 tgctacacca cttagtgtgc ggctagcata tatctgcaat ctaccacctt gattaatttg
      781 tttcgcgatc caagtgccag cttgaagcat cagcggctct tctatcctta atttctcttc
      841 caattggtga taatagtcaa aataacataa actcatttat ctctcccgcc tagctatact
      901 tgacatgaat agaaacagct gcctaatcta aggaagtaca taaaaaaaca gtagttagac
      961 agtaacacat gtggtctttg ctgtttcaaa cacaaaaaag gcattgaacg aatacgccca
     1021 ataccttgac atagcaaata aaacaacgtg cagaccatgg aaaatatcta cataggggta
     1081 gaattttcac cctagattag gtacaataaa ttaggtcgat gcactgcatt gactatggca
     1141 aaatgatcgg tcagggtgta tgtgtgtgac tggtcattcc ctgattctta gcgccaacta
     1201 agggtcaggg agcctttttt tattcagctg tttctatctg aatttattgt aagtaatctc
     1261 ttatccaagt gcaagcaaga aaatacaaaa aa
//
LOCUS       DADVXU010000227         1249 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.235, whole genome shotgun sequence.
ACCESSION   DADVXU010000227 DADVXU010000000
VERSION     DADVXU010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.235"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(221..370)
                     /locus_tag="KB953_002941"
     CDS             complement(221..370)
                     /locus_tag="KB953_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867838.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            832..1044
                     /locus_tag="KB953_002942"
     CDS             832..1044
                     /locus_tag="KB953_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867839.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
BASE COUNT          464 a          188 c          208 g          389 t
ORIGIN      
        1 ataacggaat attatcatcg aaacagctaa caaaattgtc ataaaaaacg agatggttga
       61 tattagaact tgtgtttttg tttaggtcta gagataaaga gagacaattt ccaaatatat
      121 gtaatgaata caaaaagacg tttgtcgcgg attaccgtta caaacgtctt ttattgattt
      181 tccgagatct catcagtcgc catttggaat ggttcactcc ttatctagat tcgtttaatg
      241 ctcggactgc ttgttcgatt gtaataccaa acgcaacgtt attttcgttt tgtgcatcaa
      301 tcgccataaa acggttagaa gttgtatcaa aagctacgcg gtattgcata tttgtttttg
      361 taaacgtcat aaaattcatc tcctttgtaa tgtaatttag ttcttgaaag tttcctttaa
      421 actttcgaac ttataccaag tttagcatcg tacgtttttt gatgcaatta aaaagcattt
      481 atcattttcc ttatttattt tttcttaaat ggtttagcta ttttctttta atccgataag
      541 tagcccaatc atcaaggtta caaatactat tgcttgaata taaatcatat gctcacctct
      601 ttataaataa cttagcaata taaagaaaaa aacgcacgaa caacatagga atataaacaa
      661 acgtttttgt cacaacgtct aagatcgtgt taaaaaatag ttatctaaaa agcagaaaga
      721 tactcaaaat aaaaaataaa aaaatggata aaaacgtttt caaatacaag gtaatttgtt
      781 attataaaat aaagaaaaaa caaaataaaa ataaagaaaa gagggaatga aatggtaaaa
      841 gcagaagtag gaagatctta tatatgcaaa ccgatcggag tatctcatga actaatcggc
      901 tgtattgaac gtacatatat caacacggct gtcgtgtatg ttgaaagtta tgaccaacga
      961 gactatcaac ttatcaatga aagcaagtat cgcatgctcg taaaattctg tgatattagc
     1021 gcacctgctg aattggtttc ttaaacagta tttttgagaa ttcgcaagaa ctcccttata
     1081 atataaataa agacagaagg gagcgagaag atatgccaaa aagaaaaagt tcagggaaaa
     1141 tgggaattat tcttgcagtg atctctgcaa ttcctgctat tttagctatt gttcgtgcga
     1201 tgaaggaatc aagagaaagt taagcacaca ttatgagcgt caaacaaaa
//
LOCUS       DADVXU010000228         1219 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.236, whole genome shotgun sequence.
ACCESSION   DADVXU010000228 DADVXU010000000
VERSION     DADVXU010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.236"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..460
                     /locus_tag="KB953_002943"
     CDS             <1..460
                     /locus_tag="KB953_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBB6867840.1"
                     /translation="LLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTTLFVSV
                     IYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGLLPKFM
                     KVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            469..696
                     /locus_tag="KB953_002944"
     CDS             469..696
                     /locus_tag="KB953_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6867841.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          377 a          195 c          204 g          443 t
ORIGIN      
        1 attgctatta aaatttgtcg ttttaggtat aagtgccgta tttattttgt tcggcgtact
       61 tttttatact cagatcgcta caagcgaaaa aggattcaat tttgattggc agaccacatt
      121 atttgtttct gtcatttact ttgcccttct cctagggatc gttattgctt atttcttatt
      181 gaaactattg aaaaaaatcg aagataacca agctttttcc actcaaagct taaacatact
      241 aaaaaaaatc gaacgaacca ttttgtttat tggtttcgcc ttttttggct tacttcctaa
      301 atttatgaaa gtagcggatt tagacgatgc tccaggtttg atgttggtag gaatcgctgt
      361 cgtattcatt ccttttgcag tatacacatt aacaagcgtg ctggaaaaac tgcttgaaca
      421 tgcgatttta cttaagacag atcatgattt gacagtgtga ggtgcgacat ggcaattatc
      481 gttaatttag atgtagtatt agctcaaaaa aaaatgagtg tgactgaatt gagtgagaaa
      541 gtgggcatta cgatggcaaa catctctatt ctaaaaaatg gaaaagcaaa agccatccgt
      601 ttttccactc tcgaaaaaat ctgccaagtt ttggagtgtc aaccaggtga tttgttgacc
      661 tataaaaatg actcgaatac tataccagaa gaataaaata aagtagaaca aacagtaaat
      721 aaaaaataat ctgaatgata gatgtgtgag acttttagaa gttatttacg tctattattc
      781 agatttttta tttcgattga ataccgtttt atctataaat aagatatgta ctgactcata
      841 caaagggggc cttttcttct ccatgtacta tcacttttcc tgatacaact ggtttcctgt
      901 tacgttgtag ttggatctgc ttttagtata atactttatt cattccacca gactgatagc
      961 agtttgagca gtctaaataa tcatgtagta ttacaggcca ttctgtttca taccttgaag
     1021 tgcttaaaac ttcagctaat ggcccattgt gttaactctt actccattct tttttagtaa
     1081 gctcccatat atactgtaaa gccttctata tattatgtca tgttgctttt taacatttac
     1141 tatgtagttt tgataaaatg atttgaataa cagtgatgga caattctttt ttatttttaa
     1201 cattattttg ataataatg
//
LOCUS       DADVXU010000229         1218 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.237, whole genome shotgun sequence.
ACCESSION   DADVXU010000229 DADVXU010000000
VERSION     DADVXU010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.237"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            21..974
                     /locus_tag="KB953_002945"
     CDS             21..974
                     /locus_tag="KB953_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBB6867842.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            946..>1218
                     /locus_tag="KB953_002946"
     CDS             946..>1218
                     /locus_tag="KB953_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HBB6867843.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
BASE COUNT          439 a          189 c          234 g          356 t
ORIGIN      
        1 ataggaaatt ggagggaact atggaaaact tacggactgc atttaaaaca tcagataaac
       61 taggaaaagt tgtaatgctt acaggtagaa aaggcggcat tggtaagact acagacaatg
      121 atttattagc gattgtttct agtcaacttt ttgaaaaaga cgtgttgcta attgattatg
      181 accaacaaag aaatactact tccaacattg gatcaacgta tcaaattact agctttgatc
      241 gatccatgtc tgcagcaatc aaaaaagggg actgggtttc aggtattact caagtaagtc
      301 cacatttata tattatggct ggctctcctg gatctgaaga acttaatgaa tacttgtccg
      361 aaaaatatcc tgatcgtcgt aagagatcat tggccttcat taaaccttta gaagaattga
      421 gaaaaaattt cgattatatc tttatcgact gcccaccatc aacagataat gtggttcgtg
      481 cattcttaac tgctgcagat tatattattg caatgcaaga attaaaacgg tacgcaatgg
      541 aaggaacaga agattttatt aataaagttt tagttccgat tgtcacgaat tttgaagagt
      601 ctcatcttca aatcattgga attttgcctg ttcttttctc agtgcgacgt tcctctcaac
      661 acgcaaacta tcaaaaaaca attgataagt atggtgaatc aaacattttt aaaagcattg
      721 ttaaaggctc ggatcggtta gaaatgtatg gtgagaatgg tatccaatta aatgattatg
      781 tagatcgaag atggtgggct atttttgcag acatctttac agaattagaa gaaagaattg
      841 aatactatga acaacatggt gatattgaag gatttgatta tacacctaaa tatgctgact
      901 caatggccaa tcatatccta cctcttggaa aggagattaa actaaatggc attattacca
      961 acgaacaaag ataagaagaa aattggtgct gaagatattt caattaaaga tgatattaat
     1021 gtcgagttag gggaacgaaa gtacactgcg aaagaaagaa aaccagttca agtagatcct
     1081 cccgtactaa aaaccattcg ggatcttagt tatgcaaaag atattcctat gtatgaaata
     1141 gtcaaacttg caatggacgc ctatgttgat actttaaacg acgaggaacg gattatatat
     1201 aatagacgat caaaagat
//
LOCUS       DADVXU010000230         1214 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.238, whole genome shotgun sequence.
ACCESSION   DADVXU010000230 DADVXU010000000
VERSION     DADVXU010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.238"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..814
                     /locus_tag="KB953_002947"
     CDS             <1..814
                     /locus_tag="KB953_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HBB6867844.1"
                     /translation="VKGLLNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLE
                     FKLQELELHYSYLVKITEEKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEI
                     SKLIAYMQKANADVEKELYANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNI
                     GSFGEDIYKIGMTRRLEPMDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRK
                     QEVNKINQRKEFFKVSLDEIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            886..>1214
                     /locus_tag="KB953_002948"
     CDS             886..>1214
                     /locus_tag="KB953_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867845.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVN"
BASE COUNT          535 a          140 c          200 g          339 t
ORIGIN      
        1 agtgaaagga ttactcaata aactaactat ttctaatata gactcgacta gggaaaaaat
       61 cataaaaata tttgataaaa ttaataattt attcaagaat gacaatgctg aattaaggaa
      121 gggtctttta gagtttaaac ttcaagaact agaacttcat tactcttatt tagtgaaaat
      181 taccgaagaa aaagaacaac aaaaagcaat aaaagaacag atgatagaag aggaaaaagt
      241 tcgtagagaa attgatcgtg aaaagaaacg tattgataaa gaacaacgtc aattcaattc
      301 tgaaatttcc aaattaatcg catatatgca aaaagctaat gcagatgtgg aaaaagaatt
      361 atatgcaaat aaaattcaag aactggaaga aaaattaaaa gaattagaag ttatacgtga
      421 aaatgtatta caaagagaat taaatactag agccggttat gtgtatgtaa ttagtaatat
      481 tgggtctttt ggtgaagaca tatataaaat tggtatgact agaagattag aacctatgga
      541 tagaataaaa gaattgagta gtgcatcggt tccatttgaa tttgacgttc atgcgatgat
      601 tttttcagag gacgctccgt ctttggaaac taaattgcat aatcatttta gaaaacaaga
      661 agttaataaa attaatcaaa gaaaagaatt tttcaaagtt tcactagacg aaatagagaa
      721 agttgtttta gaaaattata atggaacagt aacatttaca aaattagcta aagctgaaca
      781 atatagacga tctttagaat tgtcaaaaga ttaaatatat aaaaagcctt cgggcttttt
      841 cttttaacaa ttaataggaa cgtatattcg aaaggaatag aaataatgtg gttagaaaaa
      901 ataaatgaca aaaaatttaa gtacaccgaa cgttatacgg atcccttaac tgaaaaaaag
      961 agaaaagtgt ccgttacttt atctagcaat tcacgccaag cttggaatca agctaactta
     1021 cttttgaatg aaaaaatcgc agaaaaaata aaacgaaatg aagaattacc actaactttt
     1081 ggagaattaa aaactaaatg ggatgaaaaa tacaagccaa ctgtaaaaga aagttcctat
     1141 cgaacaactc aggtttatct atcattaatt tcaaaatata taaaagatga tgtacttgta
     1201 aaaaacgtta atag
//
LOCUS       DADVXU010000231         1209 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.239, whole genome shotgun sequence.
ACCESSION   DADVXU010000231 DADVXU010000000
VERSION     DADVXU010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.239"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..798
                     /locus_tag="KB953_002949"
     CDS             <1..798
                     /locus_tag="KB953_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HBB6867846.1"
                     /translation="MMNFIVQIFYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIR
                     EVLPVKIKWMVSILLVLLVYFSFGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPE
                     KEVILQHSSDNSEISAGRFTIWKAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDT
                     TDVNELKRAHGNMHNGFLQVLVSSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLG
                     IVLIFILSIFIDEMVEAHVLFNKRDVISIVFWYYLGSISYLYLKKEN"
     gene            800..>1209
                     /locus_tag="KB953_002950"
     CDS             800..>1209
                     /locus_tag="KB953_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867847.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTS"
BASE COUNT          406 a          143 c          203 g          457 t
ORIGIN      
        1 atgatgaatt ttatcgtaca aatattttat attacacttt cttcgtctag aggtgcccag
       61 gtagtaattt gttctttttt agtattattt cttttgttgt ttgccaattc aaaatttaga
      121 aagagcatta gagaagtact acctgtaaaa atcaaatgga tggtaagcat attattagtt
      181 ctattggtat atttttcttt tggtactaat tatgctaagg attttttagc aacaattcct
      241 gtatcgattg atagaaaagt agaagttata gagtcaaatg aatcagataa aactccactt
      301 ccagaaaaag aagtaatatt acagcattct agtgataact ccgaaatttc agctggtcgt
      361 tttacgattt ggaaagctgc gcttaaagtt ataagaccaa ctttgttatt tggttatgga
      421 gagaccgatt tctatgggaa tagcgatgca aaatttttga aaaaagtaga tcttgatact
      481 acagatgtga atgaactaaa aagagcacat ggaaacatgc ataatggatt tcttcaagta
      541 cttgtatcaa gtggtataat tgcttttata tgtatctatg ctttctatgt gttaaatatc
      601 tttagtttac tcaaaacatt ttttagaagt aaagtgaatt atgcgtttct tggtattgtt
      661 ttaattttta tactatctat ttttatagat gaaatggtgg aggctcatgt acttttcaat
      721 aagcgcgatg tcatttcaat tgttttctgg tattatttgg gaagcatttc ttacttgtat
      781 ttaaaaaagg agaattaata tgaagacagc tttcattgct tggactcctt tacatttgat
      841 taatatatta aatgtttatc aaaattttta ccgagaatca aaaggagatt tatttattta
      901 ttctgaattt gaaaacgcta gaagatatag tgagcgtata aaagaattag ggctatttaa
      961 tgatgttgtt tttgtgaatc accggaaaat cggttcttcc tttaaacgta aaattaactt
     1021 gttaactaac cataatcaat ttttctcgga tgatttatcc gtttatgatg aaatttttat
     1081 acagggggaa aattatttca caaaaatact tttctccaat gtttacaaaa aaaatccaga
     1141 tgtaaaacta aattatatag aagatggttt gggtacttat ttgaagaaga gtatattttc
     1201 aacaagtaa
//
LOCUS       DADVXU010000232         1183 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.240, whole genome shotgun sequence.
ACCESSION   DADVXU010000232 DADVXU010000000
VERSION     DADVXU010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.240"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            4..963
                     /locus_tag="KB953_002951"
     CDS             4..963
                     /locus_tag="KB953_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HBB6867848.1"
                     /translation="MNGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRNE
                     AQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKAT
                     FEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSEM
                     GVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIEN
                     ANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRADVNQAYNTSGNVGYHVSF
                     NMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          397 a          222 c          240 g          324 t
ORIGIN      
        1 ccaattaacg gatttgccta tactatcaac aatgaattta atttgggcgc aaatgaaggt
       61 agctcacaag tagcaaataa tcagtatatt ttactgcatg aaacggctaa tgaaacagca
      121 acaggacgca atgaagcgca gtatatgcaa cgttcatgga ctagcgctta tactgcttat
      181 attgtgggag acggcggaat tgtttatcaa gtcggtcaac ctggttatgt acagtacggt
      241 gctggttcgt atgctaatgc caacagtcct gtgcagattg agttacaaca cacacatgat
      301 aaagcaacgt ttgagaaaaa ctacaaggca tacgttgaat tggctagaga ttcagcaatg
      361 aaatatggta ttccattaac gttggacact ccttataacc aaccgggaat caaatcgcat
      421 ttatgggtaa cacaaaacat ctggggcgat catacagatc cttacggtta tctttctgaa
      481 atgggcgtaa gtaaagaaaa attagcatat gatttagctc atggatttac cgatgaaaat
      541 ccaacaactt ctgaaaacaa gcctgtcatt gatccaacac gagctggtgc agctaatcct
      601 acactgacag atggaacgaa ttacgcccac attgatcagt ttggagaaat cgaaaatgca
      661 aatttgcatg tagctggatg gcacattgct aactataaat acgagtatat cttcattatg
      721 gattacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagagcagat
      781 gtaaatcaag cttataatac ttctggaaat gttggctatc atgtatcttt taacatgcgt
      841 aacttcccta ataagaaagt ctatgtcatg atgcgggcaa cgaatgatcc agagggaaac
      901 actaaaggcg gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga
      961 taaaaatagc tcctcgttga ggagcattac ataactatat tgacaactat aaaaatcatt
     1021 cgataaaata gtgatgttat cgcatatctt cactatcacc cataatagtc acactccaag
     1081 ctatgcgata acaggtttgt tgccacacat tctactggtt gattgtttat ggctttatgt
     1141 ggcaacaacc agtaccctta gctcagttgg tcagagcaga cgg
//
LOCUS       DADVXU010000233         1177 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.241, whole genome shotgun sequence.
ACCESSION   DADVXU010000233 DADVXU010000000
VERSION     DADVXU010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.241"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            34..294
                     /locus_tag="KB953_002952"
     CDS             34..294
                     /locus_tag="KB953_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867849.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(656..895)
                     /locus_tag="KB953_002953"
     CDS             complement(656..895)
                     /locus_tag="KB953_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867850.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          419 a          164 c          219 g          375 t
ORIGIN      
        1 aaataatgga aaaacattaa ggatggatga aatatgaata acgaaactat cattttagga
       61 aaaaaatata tttgccaacc tattggcttg aaacaaaaag tggttggaga agtaatcagt
      121 aaattagaaa attgtgttgt gatctgcgta gacgaatatc atgacgtcga tcgcaatgaa
      181 atattagaaa aatgcggaaa agtagtggca aaatatactc acgtttatga gttggaaaca
      241 acagaatatc tatataacta tagcaacaaa tatgaacata ctttagtttc ataaagattt
      301 aaataaagtg ccttcaaaag cgagaagacc aagcttttga aggcacttta tttagttggc
      361 agtgattgtt catgattttt gatttattca aaaggttcgc ctagagaatc tgtctctgta
      421 acgaggcctg gtggagaaac tcgataaaaa cctatactgc cagaactgtc ctattttcgg
      481 atagacaaac tagtagtttt aaaaagatca tccgatccaa tcatttcaaa aaatgcaaaa
      541 ctgatatcag gatcatttag ctcaggcttg ttatcgtaaa gcatttgtaa aggttgcatt
      601 tagttaggag taaacgcgaa actatcatca ttatttgctg aagaagatga agaagctaat
      661 tcagagtttg tttgaaataa aggagacgtg ctgacagttt ctttaccctg ttttttgcag
      721 aggtagaaac ttttatatcg gaagtttcta ccggagaatt tttctcattt gtagaaattg
      781 ttgaatcgat actattcgaa atttgcgtat ctgaaggtat gttctttgat gcagaagtcc
      841 cacaagcagt aagtaacaat aaatttgata aactgataaa cagaattctt ttcattatgt
      901 acacactcct tatagtatcg tttagcatac acctatgctc agtattaggc aaaatcaatg
      961 caaaactatc aagtctttta tagtatctat tagtcttagt aatacatata tggagagggt
     1021 gatatcccag ttcaaaccag atacattggt taccatctgt attaacgatt atgcctctaa
     1081 acgctttttt tatgaaaaaa tttataagtg aaaacgtagg tatgatatta tctagaggta
     1141 atttttttgg gtatttaaat agttgctagg aggatag
//
LOCUS       DADVXU010000234         1132 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.242, whole genome shotgun sequence.
ACCESSION   DADVXU010000234 DADVXU010000000
VERSION     DADVXU010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.242"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..1121
                     /locus_tag="KB953_002954"
     CDS             <1..1121
                     /locus_tag="KB953_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBB6867851.1"
                     /translation="LETEVDKIDIQNKKISCVKKDGTRFSQEYDKVILATGSKQIMLD
                     IPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYIGVEIAEAIRKRGKEVYL
                     FDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYLGRKKIEKIVTTSGTQYS
                     MDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKDIYAIGDCATTFFTSINR
                     SSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFNYNFYAAGLNLKTARLLS
                     ESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQLCSQEDLSGLLSMFSLAI
                     EEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
BASE COUNT          437 a          138 c          191 g          366 t
ORIGIN      
        1 ttctagagac agaagttgac aaaattgata ttcaaaataa aaagattagt tgtgtaaaaa
       61 aagatggtac aagattctct caagaatatg acaaagttat attagctaca ggttcaaagc
      121 aaattatgct agatataccg gggaataatt tagctaatat tttttcgata aagaattttc
      181 caaatgcaaa agagattttt aaagctttgg atgatagtac tattaaaaaa gtaggaatta
      241 ttggagcagg atatattgga gttgaaattg ctgaagccat aagaaaaaga gggaaagaag
      301 tatatctttt cgatgttgca gatagagtgt tgtcgactta ttacgaccgt agtttttcag
      361 ataaagttga agaaatacta tcaaaaaatg gaatacatct ttgtctatca gaaagtccta
      421 aaaaatattt aggaagaaaa aagattgaaa aaatagtcac tacaagtggt acccaatact
      481 ctatggatat ggtcgtacaa gctataggat ttctatctaa tagtccaatt ggggaaaagg
      541 aactcttgag agatgttagt ggagcatatc taactaatga ataccagcaa acaaatataa
      601 aagatatata tgctattggg gattgtgcta caactttttt tacgtccatt aatagaagta
      661 gtgttgattt ttctatttct aatgctctaa gaacagctta tattgcaact caacatatta
      721 acaatcaaga ttttacacct tctggttccc aatttacaaa tggcttttct atatttaatt
      781 acaattttta cgcagcaggt ttgaatttaa aaacagcacg attattaagt gagtctatag
      841 attatataga aatagaagat ttcattagac ctgctttttt gagaaaaaat agtcgtgtaa
      901 aattaagaat tagctttgaa aaaaatacaa agagaatagt aggtgtacaa ctttgtagtc
      961 aagaagattt atctggacta ttatcaatgt tttctcttgc tatagaagaa caaattacat
     1021 tagacaaatt gaaaatgtta gattatttat ttcatcctag ttttagccaa ccctataatt
     1081 ttattttaaa ggcagtaaca ggattaaaca ttgaaacata atcgttacta aa
//
LOCUS       DADVXU010000235         1095 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.243, whole genome shotgun sequence.
ACCESSION   DADVXU010000235 DADVXU010000000
VERSION     DADVXU010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.243"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            266..>1095
                     /locus_tag="KB953_002955"
     CDS             266..>1095
                     /locus_tag="KB953_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBB6867852.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
BASE COUNT          386 a          152 c          186 g          371 t
ORIGIN      
        1 gtataattta aaggtaataa gttattcttt tattactatg tttgggtttt atagcaaaga
       61 aagtttaata agagtttgga cctagcaaac aatattaatg aggtcattaa atcttattat
      121 ctttagtgat ataacgaaat atctacacag acaaaatgat agcgtaaaat attttgtgta
      181 aatagatatt tagggtagaa aaaaagaaag tccattctgt aaaattaaag ttaccacaac
      241 cgaaatttta taggaggact ttctcatgcc caattttact acagaaatta tggaaacact
      301 aatcaataaa ggtgatttag atgaattatt tcgatgtcat ttagaattag ctgttaattc
      361 attattgcag gctgaattaa cagcttttct tgattatgaa aaatatgatc gtgctgggtt
      421 caattcaggt aattcccgta atgggaatta ttcacgttca ttcagaacag agtatggaga
      481 attaaatttg gtaattccta gagatagaaa tggtaagttt tctcaacaaa ctttacctgc
      541 ttataaaaga accaatgact ctttagaaac cactattatt cagctatttc aaaaaggaat
      601 tactatgtct gaaatctctg aattgattga aaaaatgtac gggcatcact atacgccaca
      661 aaccatttca aatatgacta aaatagtttc tgaagatatt attgccttta aagaaaggtc
      721 cttagaatcc agatactctg tcatttttat ggatgctact cacattcctt taaaaagaca
      781 aactgtttca aaagaagcag tttatattgt aattggcatt cgattagatg gaaccaaaga
      841 ggttcttggg tttagtcttg ctccaactga atctgcttat gtttggaaag agattcttca
      901 agacctaaaa gaccgtggtt taaaagaggt tttattagtc gtaactgatg gtttaagtgg
      961 cattaacgat agtattcata gtgtttatcc aaatgctcaa tttcaacaat gttgtgtcca
     1021 tatatctaga aatatcgctc ataaggttcg tgttagtgat cgacaagaaa tctgtagcga
     1081 tttcaaatta gttta
//
LOCUS       DADVXU010000236         1087 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.244, whole genome shotgun sequence.
ACCESSION   DADVXU010000236 DADVXU010000000
VERSION     DADVXU010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.244"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            60..752
                     /locus_tag="KB953_002956"
     CDS             60..752
                     /locus_tag="KB953_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBB6867853.1"
                     /translation="MRKKIIINAGVIIGLMLVIFSGKQYADNILLWGGEDNIEKINNN
                     LTILDTALENKEQKISILNSQLSSNNQTLEQLRTNIEVSKQKVASLENDKNQLVSEKT
                     NLENQLSAKDSELQGKQNEINAKIDEINQKVAEINRLNSDWNNRLQNEKNKFNEEQST
                     INSLNQQINQMLSEKNNIQNQLQATQQKLEEEKSKNSSLNNYIEKLQKAKNDVEDTAN
                     KAQQIVNEHTIQ"
BASE COUNT          481 a          120 c          162 g          324 t
ORIGIN      
        1 acgaagaaaa taatttttaa taacttattt aaggaaattt tttgaaggag aagataaaag
       61 tgagaaaaaa gattattatt aatgctggag taattatagg attgatgtta gtaattttct
      121 ccggaaagca atatgctgat aatattttat tatggggagg ggaagataat attgaaaaga
      181 tcaacaacaa tttaactata ttagacacag ctctagaaaa taaagagcaa aaaattagta
      241 ttttaaattc acaattgtct tcaaacaacc aaactcttga acaactaaga actaatattg
      301 aagtttccaa acaaaaagtg gcatctttag aaaacgacaa gaatcaatta gtatcagaga
      361 aaactaattt agaaaatcaa ttgtcagcaa aagactctga attgcaagga aaacaaaatg
      421 aaattaatgc gaaaatagac gaaattaacc aaaaagtagc tgaaataaat cgattaaata
      481 gcgattggaa taatcgttta caaaatgaaa agaacaaatt taatgaagaa caatctacta
      541 ttaatagttt aaaccagcaa attaatcaaa tgttgagtga aaaaaataac attcaaaatc
      601 aactgcaagc tactcagcaa aagctagaag aagaaaagtc aaaaaatagt agtttgaata
      661 attacattga aaagcttcaa aaagctaaaa atgatgtaga ggataccgca aataaagcac
      721 aacaaattgt aaatgaacat acaatccaat agatatagtt ttaatttaac aaatggtatg
      781 tttggagata atgagataga atataaatgt cataatatta tacaatctaa acaccgtttc
      841 cttttctatt tttgatgaca tcagtatata gctactcgaa agagtagtct ttttattatt
      901 ctcgcttatt attgatatga aaatttcgat ttgacaaaag gtaccgaata attttgtaat
      961 atgaattatt atcaagaagt aattatgatg ataacatatg gaaactagtt agtaataata
     1021 acaggtgatt aaacttttga acgacttgta attctatatt ttttctttta agtaaaaaat
     1081 ataggtg
//
LOCUS       DADVXU010000237         1069 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.245, whole genome shotgun sequence.
ACCESSION   DADVXU010000237 DADVXU010000000
VERSION     DADVXU010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.245"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..969
                     /locus_tag="KB953_002957"
     CDS             <1..969
                     /locus_tag="KB953_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBB6867854.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          368 a          185 c          214 g          302 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccat
//
LOCUS       DADVXU010000238         1063 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.246, whole genome shotgun sequence.
ACCESSION   DADVXU010000238 DADVXU010000000
VERSION     DADVXU010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.246"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(38..994)
                     /locus_tag="KB953_002958"
     CDS             complement(38..994)
                     /locus_tag="KB953_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867855.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          177 c          160 g          380 t
ORIGIN      
        1 tttagaaaaa gcagtcggac tttctaaaag aagttcacta ttcgatcctg tcgatgattt
       61 cttcaggaaa atcgtctatt tgcttgtcaa ttttgctgtc tttgtatttc tcttcttgga
      121 tagtcaacaa aatctttgta agattccccc agtctactgc tcttggcgga tcgagccaaa
      181 ccactacttg agatgcactt tcttcatata gattattcct attcgtgatt ataacatccg
      241 ggaactcagg ctttcctgcc tctatcactt ccgtgttcag attaatgtac aagcttaaat
      301 ttttcttgat aaattgatta taggcagcac cataagaaaa atcgatgatg atctttactg
      361 gttcagaaat cagttgaatg ggtatgtgat tgatcaataa taataaataa ctgaaaaaaa
      421 gaaaaaattt atcttgaagc aactctttgc gttttgacgt tagatcattt atatactcta
      481 tcaaatagca ataaaatgct ggatagtttt ctctaaaaaa agaaacatca agtggaaatt
      541 catcagcttt attaatagtg taatgcaaaa gtttaaaatg tattttggtt aactctcttc
      601 ggatcaattg gctcgtctct tctcgcaaca gattaaactg attttcaaaa ctttcaatga
      661 atcgttcatt caaccattcg attgtttcat cttcattttt tctcaagtat ttttgatcca
      721 atttatcatg aagataaagc caagagagtg tcttattgat aaaaataacg gatttttctc
      781 ctaatactcc ttttaagtgg taaaatccta tatccgaaaa aaatagcgac tgattgctag
      841 caatatctaa agaatcagct tgttcagcca acaggttgat tgctaaaaag tggaataatt
      901 tctttttttc ataagcagac agaggataat actgttttaa atcttttaac attttttctg
      961 tttttataat tataggtagc gtatacaatt ccacaaaact tatctctgtt gagttaaaaa
     1021 gattatataa aaaaagccga atgtttttag gattccctgt caa
//
LOCUS       DADVXU010000239         1049 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.247, whole genome shotgun sequence.
ACCESSION   DADVXU010000239 DADVXU010000000
VERSION     DADVXU010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.247"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            47..457
                     /gene="spx"
                     /locus_tag="KB953_002959"
     CDS             47..457
                     /gene="spx"
                     /locus_tag="KB953_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HBB6867856.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            471..827
                     /locus_tag="KB953_002960"
     CDS             471..827
                     /locus_tag="KB953_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HBB6867857.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          360 a          157 c          204 g          328 t
ORIGIN      
        1 aagaaattac tgtagaattg gattataatt aaaaaggagt gagaaaatga taaaagtata
       61 tacttcgcct agctgtactt cctgtagaaa agcaagagca tggttgcaag cgaatcaact
      121 tgaatttgaa gagaagaata tttttgccga accgttaaca gaaagtgaga tcaaaaagat
      181 cttggcagcg actgaaggag gagttcaaga tattatttcc actcggtcga aaatttacga
      241 aaagctgggc atagatttta acgaattaac tttaaagcaa atgatcgtct tattcaaaga
      301 atatccttca ttattgagac gacctatttt gattgatgaa aaaagaatac taatcggatt
      361 taatgaagat gagatccggt catttgtccc tcgtgaaatt cgatgtgttg ccagaaatct
      421 gatctattta caaaacaata acatcttaaa ttcataggga gggagccttc gtggaagtga
      481 aagtctatgg atcagcgcac tgtgtaacaa gtctgaaggc gaaacaatgg ttagaacacc
      541 atcaattgcc ttatcaattt gttgacttag aagaacgcga tttaagtaca gaagaagcag
      601 aagaattatg cagttttgaa gaagtgcaag cagaacagct atttgcgact tggtctgagg
      661 cttttcacaa cattgcgatt gacctttcct gtgtaggaaa aaaacaactg atttttttat
      721 gcacacaaca taccaatctc ctcagacgtc ctttgattat tatcgatgaa gttctattta
      781 ttggatacaa tgagcagttg atggaacagc ttatcttaaa taaatagact ttaactgttt
      841 taatcaagtc actgagcatt tcatacagtt gtaacagtcg aacacttagc atcaagcgtg
      901 ttatgtgttc tttttttgac tttatttttc tactatagag acatgctcaa tttatgtggt
      961 caaaattcaa atctaggtgg ggatatagag ttaaaaatat attgacatgt gtaaatttac
     1021 atgatatatt catttctgtt gttttaagt
//
LOCUS       DADVXU010000240         1045 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.248, whole genome shotgun sequence.
ACCESSION   DADVXU010000240 DADVXU010000000
VERSION     DADVXU010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.248"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="KB953_002961"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="KB953_002961"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HBB6867858.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          376 a          132 c          182 g          355 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaac
//
LOCUS       DADVXU010000241         1035 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.249, whole genome shotgun sequence.
ACCESSION   DADVXU010000241 DADVXU010000000
VERSION     DADVXU010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.249"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..>1035)
                     /locus_tag="KB953_002962"
     rRNA            complement(<1..>1035)
                     /locus_tag="KB953_002962"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          218 a          324 c          234 g          259 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg ccttt
//
LOCUS       DADVXU010000242         1033 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.250, whole genome shotgun sequence.
ACCESSION   DADVXU010000242 DADVXU010000000
VERSION     DADVXU010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.250"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            78..193
                     /gene="rrf"
                     /locus_tag="KB953_002963"
     rRNA            78..193
                     /gene="rrf"
                     /locus_tag="KB953_002963"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            203..276
                     /locus_tag="KB953_002964"
     tRNA            203..276
                     /locus_tag="KB953_002964"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:237..239,aa:Asn,seq:gtt)
     gene            316..405
                     /locus_tag="KB953_002965"
     tRNA            316..405
                     /locus_tag="KB953_002965"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:350..352,aa:Ser,seq:gga)
     gene            420..491
                     /locus_tag="KB953_002966"
     tRNA            420..491
                     /locus_tag="KB953_002966"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:453..455,aa:Glu,seq:ttc)
     gene            507..579
                     /locus_tag="KB953_002967"
     tRNA            507..579
                     /locus_tag="KB953_002967"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:540..542,aa:Val,seq:tac)
     gene            589..661
                     /locus_tag="KB953_002968"
     tRNA            589..661
                     /locus_tag="KB953_002968"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:622..624,aa:Asp,seq:gtc)
     gene            697..772
                     /locus_tag="KB953_002969"
     tRNA            697..772
                     /locus_tag="KB953_002969"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:730..732,aa:Phe,seq:gaa)
     gene            774..854
                     /locus_tag="KB953_002970"
     tRNA            774..854
                     /locus_tag="KB953_002970"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:808..810,aa:Tyr,seq:gta)
     gene            861..934
                     /locus_tag="KB953_002971"
     tRNA            861..934
                     /locus_tag="KB953_002971"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:893..895,aa:Trp,seq:cca)
     gene            937..1009
                     /locus_tag="KB953_002972"
     tRNA            937..1009
                     /locus_tag="KB953_002972"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:970..972,aa:His,seq:gtg)
BASE COUNT          244 a          231 c          277 g          281 t
ORIGIN      
        1 actcggaaaa aaacttatgt gacttcaaat ccagttttga gtgaacaaaa tttactcaat
       61 aaaagataac accacagtgt ggtggcgata gcgagaagga tacacctgta accatgccga
      121 acacagaagt taagcttctt agcgccgatt gtagtgaggg gttgcccctt gtgagagtag
      181 gacgtcgcca cgctgatgtt attccggcat agctcagttg gtagtagcgc atgactgtta
      241 atcatgatgt cgtaggttcg agtcctactg ccggagttct cattttgaga aagagtattg
      301 agcttctttc actctggaga gttgtccgag aggccgaagg agcatgattg gaaatcatgt
      361 agatggtgcc aaactgtctc aagggttcga atcccttact ctccgtagca aatttacctg
      421 gcccgttggt caagcggtta agacaccgcc ctttcacggc ggtaacacgg gttcgaatcc
      481 cgtacgggtc attttaattg aatgttggag gtttagctca gctgggagag catctgcctt
      541 acaagcagag ggtcagcggt tcgatcccgt taacctccat aatgaactgg ttccgtggtg
      601 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
      661 gtcgttcttg ttattcaatt aataatttta aaatatggcg cggtagctca gttggtagag
      721 caacggattg aagctccgtg tgtcggcagt tcgattctgt cccgcgccac catggaggag
      781 tagcgaagtg gctaaacgcg acggactgta aatccgttcc ttagggttca gtggttcgaa
      841 tccactctcc tccattttat aggggcatag tttaaaggta gaacagcggt ctccaaaacc
      901 gttagtgtgg gttcaattcc tgctgcccct gccatggcga atgtggcgaa gtggttaacg
      961 caccggattg tggctccggc attcgtgggt tcgattccca tcattcgccc tttttattct
     1021 tggggtatag cca
//
LOCUS       DADVXU010000243         1033 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.251, whole genome shotgun sequence.
ACCESSION   DADVXU010000243 DADVXU010000000
VERSION     DADVXU010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.251"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            101..841
                     /locus_tag="KB953_002973"
     CDS             101..841
                     /locus_tag="KB953_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867859.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT          380 a          185 c          199 g          269 t
ORIGIN      
        1 ttcatcgcta ttttcttaaa ataatcagcg cattgctact gaaaacaatg aatctgtgct
       61 attattgaga aaagttccat ttttgagaag gatggtttga atgaaagaaa tcagacgaaa
      121 acatttccgt tttcctgcgt acgatgatga gttaggcgta aaattaacca acgacaagaa
      181 gcgagtatta tttgacggac aagaagatct tttgactgaa cagaatcagt ccccgtttga
      241 acagatgaaa gaatttcatt tcacgaacac gaacaccgag aaaatccgtc gcaagcgtcg
      301 tgcaacaaat ggattgaagt cagctccgcg taaggaaaat ttggaaaagc ataaaggaaa
      361 attaccggat tataccggcc attaccaatc agaaatcaca tcgacaggac gaaaaaaact
      421 ttttggtgat tcctctcgtt ctgtcagaca aagtgaaaca aggacagagt ccaagcatac
      481 tacacgaaac gcatctgttg acacgaaggt gcgggagcgt tcttattttg ttcctaaata
      541 tgtacccgca tcgattatcc cagaccctag agaaagcaaa atttctgagg atcatttaat
      601 cgaatcgatg aaaaaaacgg atgactctta tctgttgttc gatacagaac cgacgccgta
      661 tcaagaaaaa agaaatggtg agccgtctgt acgaacattc aatcgaacaa aaacgttaga
      721 ggaaacaaag cagtcagaag aaaaaaaatc gaaaaaaagc catggtatct tagaacgctc
      781 attacaagga ttgatcgatg atcaaggaaa ctctttggga gaaaatagct attttaaata
      841 agtgatatac aaatttatat tcggctacag ttggaaagga aacgctgcgg cgtttcctta
      901 ttttatactc tatccgaaag tttatatcgt atcctcaaat ttttttgagt ttaagcgaat
      961 tttttctccc aataagtaag aaaataatgg caaaataaaa aaacaaacta ttttcgcaga
     1021 tcgtttgcaa aaa
//
LOCUS       DADVXU010000244         1026 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.252, whole genome shotgun sequence.
ACCESSION   DADVXU010000244 DADVXU010000000
VERSION     DADVXU010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.252"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            46..822
                     /locus_tag="KB953_002974"
     CDS             46..822
                     /locus_tag="KB953_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HBB6867860.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            837..>1026
                     /locus_tag="KB953_002975"
     CDS             837..>1026
                     /locus_tag="KB953_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6867861.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIIT"
BASE COUNT          405 a          161 c          190 g          270 t
ORIGIN      
        1 agttgttaaa tttgtttacg aatataaaag aaaggaatga gaaatatgaa cacaccgcaa
       61 atttttaatt tcgaacaaaa cgaagttcgg acaattttag taaatgatga accatatttt
      121 gtaggcaaag acgttgcgag tgttttgggt tattcaaaca ctaaagatgc tttgtcgcgg
      181 catgtagatt tggaagataa gatggggtcg cgaatcacga cctcaggtca atcaagagag
      241 atgacaatca tcaacgaatc tggtttgtac agtttaatct taaaatcaaa acttccctct
      301 gccaaaaaat ttaaacgttg ggtaacaagt gaagtgttgc cagcaattag aaaacatgga
      361 ggttatctaa ctccagaaaa agtagaagaa gctttgctta atccagatac aatcattcaa
      421 ttagcaactc aactaaaaga agaaagaact ggaagattaa tcgcagaaca aaagattgca
      481 gagtacgaac ctaaaatctc ctatttagat agcatattat cttctacaga ttcagtaaca
      541 attagtcaga ttgcagcaga ttatgggatg tctccacaac agatgaataa attacttcat
      601 aaactaggta ttcagaaaaa agtcggtaac caatggttat tgtgcaaaaa acacatgaac
      661 caaggataca caaaatctca tacaactgag atcccgaaag ccgatggtgg cactaaaatt
      721 gtaatgaata ccaaatggac acagaaaggg cgtctattta tctacgaatt actaaaaaaa
      781 gaaggatatt accctcaaat ggatttagag gaaattggtt agaaaggagt tttagtatga
      841 ctgacattgc agaaatcact caacgagata gagaaaaaat caaagaatat gtcgaaagtt
      901 caaagttctt aacttacacc atgcttgctg aaagatttgg aattagcaaa agctacttat
      961 ctttaatttt aaacggtaaa aagacttctg cagaagcaaa cagaattata gattcgatta
     1021 tcacta
//
LOCUS       DADVXU010000245         1020 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.253, whole genome shotgun sequence.
ACCESSION   DADVXU010000245 DADVXU010000000
VERSION     DADVXU010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.253"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..520
                     /locus_tag="KB953_002976"
     CDS             <1..520
                     /locus_tag="KB953_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBB6867862.1"
                     /translation="LEFNLSFGDNKIIFAEDNWFQLQVFMTNTRICFVDKLCYYYRQQ
                     NNSIMHKPKSNLVFRHGNMIADYEKLIRQNGNNIIDKKACMLVAFDVLIMEIFNQKNV
                     GGTCEDYKNALVSLKHHKILRENISNIVRMKAYELEPNMRKKLALVTISIMFNFKMYK
                     TAYNMLWRIYNR"
     gene            534..>1020
                     /locus_tag="KB953_002977"
     CDS             534..>1020
                     /locus_tag="KB953_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBB6867863.1"
                     /translation="MRKLTFVIGIMGKGGAERVIANLANNFIQDNWEIDIITIYGNRQ
                     DYELNRKIQVHPIICKSSFRAFRPLERFKELRKKIREIQPDLVISFLADVNIHVIISL
                     LGVKQKLIVSERNDPQRNPEEPWIRKIRDFLYKNVDGIVFQTPDAKKYFSKINDKKVK
                     TI"
BASE COUNT          411 a          104 c          186 g          319 t
ORIGIN      
        1 attagagttt aatttatcat ttggtgataa caaaataatt tttgcagaag ataattggtt
       61 tcaattacaa gtatttatga caaatacaag aatctgtttc gtagataaat tgtgttatta
      121 ttatcgccaa caaaataatt ctataatgca taagccaaaa agtaatttgg tttttagaca
      181 tggaaatatg attgctgatt atgaaaagtt gataaggcaa aatggaaaca acataataga
      241 taaaaaagct tgtatgttgg ttgcatttga tgtactaatt atggagattt ttaatcaaaa
      301 aaacgttggt ggaacatgtg aagattataa gaatgcatta gtttctttga aacatcataa
      361 aatattgaga gaaaatattt caaatatagt tagaatgaaa gcatatgaac ttgaacctaa
      421 tatgagaaag aagttagccc ttgtgaccat tagtataatg tttaatttta aaatgtataa
      481 aacagcgtat aatatgctct ggagaatata taatcgataa agaggtagga aatatgagga
      541 aattgacctt tgtgatagga attatgggaa aaggaggggc agaacgagta attgcgaatt
      601 tggcaaataa ttttattcaa gataactggg aaattgatat tataactata tatgggaatc
      661 ggcaagacta tgaactgaat agaaaaatac aggtacatcc aataatatgt aaaagttcgt
      721 ttcgtgcatt tagacctttg gaacgcttta aggaacttag gaaaaaaata agggaaatac
      781 agcctgatct ggttatttcg ttccttgctg atgtaaatat ccatgtcatt atttcattgt
      841 taggagtaaa acaaaaatta attgtttctg aacgaaatga tccacaacgg aatccagaag
      901 aaccatggat taggaaaatt agagattttc tttataaaaa tgtagatgga attgtttttc
      961 aaacaccaga cgcaaaaaaa tattttagta agatcaatga taaaaaagta aaaacaatta
//
LOCUS       DADVXU010000246         1000 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.254, whole genome shotgun sequence.
ACCESSION   DADVXU010000246 DADVXU010000000
VERSION     DADVXU010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.254"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            369..1000
                     /locus_tag="KB953_002978"
                     /pseudo
     CDS             369..1000
                     /locus_tag="KB953_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          181 c          183 g          294 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata
      301 taggctctat aatttatggg actgatgaat caaaattgat tcttcagtcc cattgttatt
      361 ttaggtatat gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa
      421 ggaccctgcc aaaagtattg atggtggatg aatttcgttc gcacgcttca attgtggata
      481 aaatgagctt tatttgtgcg gatggcgaga ctggaaaact cattgatatt ttgcctacac
      541 gaaaattgtc tcgattaaca cactatttta gtcgctgtgc aaatcctgaa gcggtggaat
      601 atttagtgac agatatgaac gccgcctact tccaactgac aaaacgtgtc ttaccaaatg
      661 cgaaagtcgt cattgattgt tttcatatca tcaaacattt gaatcaggcg ttcaatgatt
      721 ttcgtgtaca agaaatgaaa agattacacc agttagggca acgacgggaa gccgagaaag
      781 tgaaaaagaa ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac tatgattaca
      841 aaacatggcg cagttttcgt gcacctaaat acccgctact cactgaagca atgatgattg
      901 atcgattgct ggcattttca acgcctttaa aggacgccta tacttttttc catgagctga
      961 ccgaagcctt tagagacaag gaatcagact tattttttac
//
LOCUS       DADVXU010000247          986 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.255, whole genome shotgun sequence.
ACCESSION   DADVXU010000247 DADVXU010000000
VERSION     DADVXU010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.255"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..>986
                     /locus_tag="KB953_002979"
     CDS             <1..>986
                     /locus_tag="KB953_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBB6867864.1"
                     /translation="LEPGDFIMSGAGTIGRISRVPKQIKPGVFNQALIRLRINKEITD
                     SEYFIQFIRADFMQRKLTGANPGSAITNLVPMSEVKKWIVQFPILEEQKKIGNFFKQL
                     DDTIALQQRKLDLLKETKKGFLQKMFPKNGAKVPEIRFPGFTEDWEQRKFKEFSKKTG
                     KKNTKDLDFPAYSVSNKAGLISQTEQFDGSRLDDLEKTNYKFVEPNEFAYNPARVNVG
                     SIAFNNLGMTVIVSSLYVVVKMSEDLDNEFILQFIKSPTFIKEVKRNTEGSVREYLFY
                     ENFANIKFPFTRNKEEQQKIGAFFKQLDDTIALHQRKLDLLKETKKGFLQKM"
BASE COUNT          375 a          159 c          189 g          263 t
ORIGIN      
        1 ttagaaccag gcgattttat catgagtggc gctggaacaa ttgggagaat ttcaagagta
       61 ccaaagcaaa taaaaccggg tgtattcaat caggctttga ttagacttag aataaataaa
      121 gaaatcacag attcagaata ttttattcaa tttattcgtg cagattttat gcaaagaaaa
      181 ctcacaggag ctaatccagg ttcagcaatt acaaatctcg taccaatgtc agaagtgaaa
      241 aaatggatag tacaatttcc aatattagaa gaacaaaaga aaattgggaa cttcttcaaa
      301 caactggacg acactatcgc tcttcaacaa cgtaagctag atttattaaa ggaaacgaaa
      361 aaaggattct tacaaaaaat gttccctaaa aacggagcaa aagtgccaga aattcggttt
      421 cctggattta cggaagattg ggaacagcgt aagttcaaag agtttagtaa gaagactgga
      481 aaaaagaaca cgaaagattt agacttccct gcctattcgg taagtaataa agcaggttta
      541 atttctcaaa cagaacagtt tgatggtagt aggttggatg atttagaaaa aacaaattat
      601 aaattcgttg aaccaaatga attcgcctat aatccagcaa gagtaaacgt aggatcaatt
      661 gcatttaaca atcttggtat gacggtcatt gttagctctc tttatgtagt tgtgaaaatg
      721 agcgaggact tagataatga gttcatactt cagtttatca agtcaccaac atttatcaaa
      781 gaggtcaagc gcaacactga aggaagcgta cgagaatact tattctatga gaatttcgct
      841 aatatcaagt ttccttttac taggaataaa gaggaacaac aaaaaatcgg cgctttcttc
      901 aaacaactcg acgacacgat cgctcttcat caacgtaagt tagatttgct gaaggaaacg
      961 aaaaaaggct tcttacaaaa gatgtt
//
LOCUS       DADVXU010000248          983 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.256, whole genome shotgun sequence.
ACCESSION   DADVXU010000248 DADVXU010000000
VERSION     DADVXU010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.256"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(34..107)
                     /locus_tag="KB953_002980"
     tRNA            complement(34..107)
                     /locus_tag="KB953_002980"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(71..73),aa:Ile,seq:cat)
     gene            complement(662..976)
                     /locus_tag="KB953_002981"
     CDS             complement(662..976)
                     /locus_tag="KB953_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867865.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          363 a          154 c          154 g          312 t
ORIGIN      
        1 tctaacacag aacgattcaa ttggctacat taatgtaccc tgtaggactc gaacctacga
       61 ccggacggtt atgagccgtc tgctctgacc aactgagcta agggtagtgg ttgttgccac
      121 ataaagccat aaacaatcaa ccagtagaat gcgtggcaac aaacctgtta tcgcatatct
      181 tggagtgtga ctatttatga gtgatagtga agatatgcgt ctccatatat gtatggttga
      241 gacagataaa catgtatgaa aaacgttgtt aaatcaacgt ttttttattt tggtcaagga
      301 gagaaaagta gagaaataca aaattttttg cattgatttt gcattcaaaa aaacattcaa
      361 acatttagaa atccccaata cgtattcagg aaaatctatt ttaaggttta caaaaagaac
      421 ttttgttcgt acactctttt tgaggtgaac ttttatgctg atggaaggaa aaacacaatt
      481 atggtttaag catgttcctt caaataagtt ttttaattat aaactctaga gaaattaatt
      541 catcctaaca taaaataatt gttcactcaa aagtttttct agaacaatta aaacagcagg
      601 cttcacattc gctatacatt tgtaagaagt aataattcct gtactaaaag gcataattgc
      661 tttaattatt ttttgacttc tcttcaaaca caagaagtag ttgtgtaata attgcagaaa
      721 taaggattcc aataatagat ataaaatttg acatggaaac gtctataaca ttaaaagaat
      781 aaagtgttga agaaagtaaa ctgattgaaa taacccctcc gtaaaaaata gtctgtgaat
      841 atacccactt tttttcagaa gacgtacttc gtttggttct aaaacctatc cctgcatttc
      901 tactcttatc aaaaaaccaa atatttagaa gtaataatat tgctattatt aagataaaat
      961 tactgtaaaa atccaaaagc tat
//
LOCUS       DADVXU010000249          979 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.257, whole genome shotgun sequence.
ACCESSION   DADVXU010000249 DADVXU010000000
VERSION     DADVXU010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.257"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            100..>979
                     /locus_tag="KB953_002982"
     CDS             100..>979
                     /locus_tag="KB953_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBB6867866.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSAASKRNNIP"
BASE COUNT          342 a          171 c          179 g          287 t
ORIGIN      
        1 ttattacgcc gattgtaaaa ttaagctaga caactaaaaa agtcatttgt gctacactca
       61 aaatagttcc gaccaaagaa ttataaggag tgagtacaaa tgacctatac ccatcttaca
      121 acggatgaac ttgtaataat agagtcttat ttcaaaatga atcaatctgt tgctaaaact
      181 gcccattgct tgaatcgttc aagacaaacg atccataaag tatatctgtt cttcaagcaa
      241 ggaaaatcag ccttagaata ttatcaacag tataagaaaa acaaatcaaa ctgtggtaga
      301 cgtccgcttg ttttacccga ggaacaatca gaatatattc aaagaaaggt tgttcaagga
      361 tggacacccg atgtgattgt tggtcgtgca gcgtttccta ttagttgttc tgctcgtacc
      421 atttatcgta tgttcaaaaa ggggctattt gattcttctg acttaccgat gaaaggcaag
      481 cgtaaaccga atggacatca agaaagacgt ggaaaacaaa ctttccgccg ctctattcat
      541 gaacgtgaaa aggattatag ccaattctca aatgagtttg gtcaccttga aggtgacact
      601 atcgtaggtc tgaaacataa aagtgctgta attaccttag ttgaacgatt atcaaaagtt
      661 atcatcacat tgaaaccgtg tggtagacaa gcgattgata ttgaaaaaaa attaaatcaa
      721 tggtttgaat ctgtaccgaa aaacctattc aaatccatca cttttgattg tggaaaggaa
      781 ttttcaaatt ggaaacagat cagtaatgtc aatgatattg ccatttattt cgctgatcca
      841 ggaacgccgt ctcaaagagg cctaaacgag aattctaacg gattgttacg tagagatggt
      901 ttattgaaat ctatggattt caattcagta gatgaatttt ttattcaatc tgccgcatct
      961 aaacgaaata atattccta
//
LOCUS       DADVXU010000250          966 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.258, whole genome shotgun sequence.
ACCESSION   DADVXU010000250 DADVXU010000000
VERSION     DADVXU010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.258"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            76..768
                     /locus_tag="KB953_002983"
     CDS             76..768
                     /locus_tag="KB953_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBB6867867.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSWKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          421 a          146 c          163 g          236 t
ORIGIN      
        1 ttaaaaacgc ttgacattgt gtttgagatc acttactttt agcatgtaaa agtattggat
       61 taaggagcta tgcaaatgaa aaagaaattt attactggtg ggattgtcgt tgcatttcta
      121 tgtctaattg gctacacagg cacgcggtac gcaaatgaaa ttattttgtg gggtggagag
      181 acagatattg atacaatcaa tgagaatctg acacagcttg atcatacatt gacagataaa
      241 gagcaagcga ttacagatct taccgctcga ctagcacaaa aagaacagac attgcaagat
      301 tcaaaggcag atgtccaaga ataccaaaca aaagtaaatg aattagaaaa ccaaaaaaat
      361 caatcggcag cagaaaaaca aaacttagaa agtcaattag tcagcaagga tgcacaacta
      421 caaagcaaac aaaatgaaat caatgtcaaa ctagatgaga tcaaccaaaa aaatgatgaa
      481 atcaatcatc tgaatgcaga ttggacagca agactacaag aagcacaagc aaaactttca
      541 gaattacaaa atactgtaaa tggtttgaat caacaaatcg caacactgac acaagaaaaa
      601 acaaatgtag aaactcagtt aacagataca caaaagaaat tagacgaagc aactgctgaa
      661 aactcaagct ggaaacaata tattcaaaag ttagaaaaag caaaagatga agtggaagat
      721 acagctgata aatctcagca aattgttgaa gaacatttgc gtaaataatc tttcttaaca
      781 agtaaaccta aattagtaaa gaagcaacag taatctatac taagtagttc agttttggag
      841 tacaaaatga caaaatgtac ttcaaaatgg actacttttt tcattttaac ttacatacca
      901 aattacatac aaaatgattt ataaaatgca attgaaaatg ataattgaat gaaaatttgt
      961 gatttt
//
LOCUS       DADVXU010000251          943 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.259, whole genome shotgun sequence.
ACCESSION   DADVXU010000251 DADVXU010000000
VERSION     DADVXU010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.259"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..>943
                     /locus_tag="KB953_002984"
     rRNA            <1..>943
                     /locus_tag="KB953_002984"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DADVXU010000252          917 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.260, whole genome shotgun sequence.
ACCESSION   DADVXU010000252 DADVXU010000000
VERSION     DADVXU010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.260"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..>917
                     /locus_tag="KB953_002985"
     CDS             <1..>917
                     /locus_tag="KB953_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBB6867868.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLP"
BASE COUNT          316 a          176 c          166 g          259 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcct
//
LOCUS       DADVXU010000253          880 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.261, whole genome shotgun sequence.
ACCESSION   DADVXU010000253 DADVXU010000000
VERSION     DADVXU010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.261"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..714
                     /locus_tag="KB953_002986"
     CDS             <1..714
                     /locus_tag="KB953_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HBB6867869.1"
                     /translation="STLSRNKKYIENETDSVVLVSSEKVIDICENKILTSAFFEQNGF
                     KTPEILNSSEITKFPVFIKPLNGSSSVNTFIVNNEKELLFFSEYIDNPLIQEYILGEE
                     YTIDVCLNFDSTPVSIVPRKRIATRGGEILKGQIIKDRELIETTKKMLEKLNAIGHIT
                     IQCIKNEKGIFYLEINARYGGGAPISIKSGANSPEYIIKMIDSKKNLRYRENYLDKMI
                     ALRYDQAIYLDEKGNVYND"
     gene            686..>880
                     /locus_tag="KB953_002987"
     CDS             686..>880
                     /locus_tag="KB953_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase"
                     /protein_id="HBB6867870.1"
                     /translation="MKKEMYIMIKAVIFDLDDTLVSEYDYVKSGYKCVARRLAVQKIF
                     PATFDEIYRVLLDLFAEDSKN"
BASE COUNT          349 a           98 c          150 g          283 t
ORIGIN      
        1 tcaacattat caagaaataa aaaatatatt gaaaatgaaa cagactcagt tgtattagtt
       61 tcttcagaaa aagtcatcga catttgtgag aacaaaattt taacttctgc tttttttgaa
      121 caaaatggtt ttaaaactcc tgaaatttta aactcttctg aaattacaaa atttcctgta
      181 tttataaaac ctctgaatgg tagttcatct gttaacacat ttattgtaaa taatgaaaaa
      241 gaattgttgt tttttagtga atatatagat aatcctctta ttcaagagta tattttaggt
      301 gaagaatata ctatagatgt ttgcttaaac tttgattcga caccagtaag tattgttcct
      361 agaaaaagaa tagcaactag aggtggggaa atactgaagg gtcagattat taaagataga
      421 gaactaattg agactacgaa aaagatgttg gaaaaattaa atgcaatcgg acatattact
      481 attcaatgta taaaaaacga aaagggaata ttttatttag aaataaatgc aagatacggt
      541 ggaggagccc ccattagcat taaatcagga gctaattccc ctgaatatat tataaagatg
      601 atagattcaa aaaaaaattt gagatacaga gaaaattatt tagataaaat gatagcactg
      661 agatatgatc aagcgattta tttagatgaa aaaggaaatg tatataatga ttaaagcggt
      721 gatctttgat ttagatgaca ctcttgtgtc tgaatatgat tatgtaaaaa gtggatacaa
      781 atgtgtagct agaagattag cagtgcaaaa aattttcccg gctacttttg acgaaatata
      841 tagagtcctt ttagatcttt ttgctgaaga tagtaaaaat
//
LOCUS       DADVXU010000254          879 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.262, whole genome shotgun sequence.
ACCESSION   DADVXU010000254 DADVXU010000000
VERSION     DADVXU010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.262"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            50..>879
                     /locus_tag="KB953_002988"
     CDS             50..>879
                     /locus_tag="KB953_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HBB6867871.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
BASE COUNT          305 a          131 c          160 g          283 t
ORIGIN      
        1 ctgtagaata gagttaccac aacacattca cagaaaagag gacttccata tgaacgattt
       61 tactacagaa attatggaaa tactaatcaa taaaggtgat ttggatgatt tgtttcgtcg
      121 tcacctagaa ttggctatta acgctttatt acaagctgaa ttaacggtat ttcttgacta
      181 tgaaaagtat gatagagctg gatttaattc aggtaattcc cgtaatggga attattcgcg
      241 ctcttttaaa acagagtacg gtgaattgaa tttagtaata cctagagata ggaatggaga
      301 cttttctcaa caaactttac ctgcttataa aagaaccaat gactctttag aaaccactat
      361 tattcagcta gttcaaaaag gaattactat gtccgaaatc tctgatctaa tcgaaaaaat
      421 gtatggtcat tattacaaac cacaaactat ttcaaatatg agtaaaattg tgtctgaaga
      481 tgtttcggca tttaaagaaa gaactttaga agctaaatac tcagtcattt ttatggatgc
      541 tactcatatt cccttaaaaa gacaaaccgt atcaaaagaa gctgtctata tagtaattgg
      601 tatcaggttg gatggaacta aagaggttct aggatttact atagctccaa ctgaatctgc
      661 ttatgtttgg aaagagattc ttcaagatct aaaagaccgt ggtttagaag aggttttgtt
      721 agtcgtaaca gatggtttaa gtggtattga cgatagtatt catagtgttt atccaaatgc
      781 tcaatttcaa caatgttgtg tccatatatc tagaaatatc gctcataagg ttcgtgttag
      841 tgatcgacaa gaaatctgta gcgatttcaa attagttta
//
LOCUS       DADVXU010000255          857 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.263, whole genome shotgun sequence.
ACCESSION   DADVXU010000255 DADVXU010000000
VERSION     DADVXU010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.263"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..116
                     /locus_tag="KB953_002989"
     CDS             <1..116
                     /locus_tag="KB953_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HBB6867872.1"
                     /translation="KILIIDDVYTTGQTLFHAADCLQNAQPKIIRTISLAR"
     gene            244..795
                     /gene="raiA"
                     /locus_tag="KB953_002990"
     CDS             244..795
                     /gene="raiA"
                     /locus_tag="KB953_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBB6867873.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
BASE COUNT          306 a          151 c          177 g          223 t
ORIGIN      
        1 aaaaaatatt gattattgac gatgtctata caacaggaca gacgcttttt catgcagcag
       61 attgcttgca gaacgcacag ccgaaaatca tccgaaccat ttctttggca agatgaatca
      121 gatgtggaaa tttgatagcg atttaatttg caaataacct agagttcgat ataatagaat
      181 taagaagagg gaaagagtgg tccttctcct tcttgattgt ggtagacgaa aggggtaatt
      241 tttatgttta gatataatgt acgcggcgaa aatatcgaag taactgaagc gattcgcgac
      301 tacgttgaga aaaaagtagg caaactagaa cgctatttca gtgacgcacc cgatgcaacg
      361 gcgcatgtga acttaaaagt ttatacagaa aaaacagcaa aagtggaagt aacgatccca
      421 ttaccttatc ttgtattacg tgccgaagaa acttctccag atttatacgc aagtatcgat
      481 ttggttgttg acaaattgga acgccaaatc cgcaaattca aaacaaaaat taatcgtaaa
      541 tcccgtgaaa cgggcatgaa tactgcagat gctgctgctt tattcaatga agaggacgaa
      601 aacgaaaatg attctgaatt agatatcgta cgtacgaaac gcctttcatt gaaaccaatg
      661 gatagtgaag aagcagtctt acaaatgaac atgctgggac ataacttctt tatttttgaa
      721 gatgcagaaa caaatggtac aagtatcgtt taccgccgta aagatggaaa atacggactg
      781 attgaaacag actgatcagc cttacttaaa taacaacgaa aacttgcagc agctactgac
      841 agctgccgca agttttt
//
LOCUS       DADVXU010000256          812 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.264, whole genome shotgun sequence.
ACCESSION   DADVXU010000256 DADVXU010000000
VERSION     DADVXU010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.264"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..673
                     /locus_tag="KB953_002991"
     CDS             <1..673
                     /locus_tag="KB953_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBB6867874.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          236 t
ORIGIN      
        1 tagcggtaaa tactatattt ctttgttgtg taaagaagaa attatcgaat tacctaaaac
       61 taattccaca attggtattg acttaggtat tatggacttt gcgattcttt ctgatggaca
      121 aaaaattgat aataataaat tcacgtccaa aatggaaaag aaactaaagc gtgaacaacg
      181 caaattgtct aggcgtgctt tattggctaa aaataaaggt accaatctat ttgaagctaa
      241 aaattatcaa aaacaaaaac gcaaagtagc tagattgcat gaaaaagtaa tgaatcaacg
      301 tactgatttt ctgaataagt tgagtacaga aatgatcaaa aatcacgata ttatctgtat
      361 tgaagactta aacacaaaag gtatgttgcg aaatcataaa ttagctaaaa gtatttctga
      421 tgtttcttgg tcaaagtttg tgactaaatt acaatacaag gctgactggt atggacgaaa
      481 aatcatcaaa gtagataaat ggtttccgtc tagtcagatt tgttcagagt gcggacataa
      541 agatggcaag aaatctcttg aaattcgaga atggacttgt cctgtttgtc atactcatca
      601 tgatcgtgat atcaatgcta gtatcaatat tctgaccgaa ggtctaagac tacattctat
      661 gggattggct tagatccaat catcaagaac cgtaggaact acggggatag cttggtaaat
      721 aagagacacc gctgttagta aagaaatacg ctatcaagta tgctctattc ccaagaagct
      781 cccacttcaa gcgttaagaa ccgtcaggtt ta
//
LOCUS       DADVXU010000257          809 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.265, whole genome shotgun sequence.
ACCESSION   DADVXU010000257 DADVXU010000000
VERSION     DADVXU010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.265"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(38..283)
                     /locus_tag="KB953_002992"
     CDS             complement(38..283)
                     /locus_tag="KB953_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6867875.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(277..678)
                     /locus_tag="KB953_002993"
     CDS             complement(277..678)
                     /locus_tag="KB953_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBB6867876.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
BASE COUNT          263 a          141 c          109 g          296 t
ORIGIN      
        1 cttttaaaca gaaaaagtgg aaaggggatt tttttaatta gttaatttta aattttaaga
       61 tcgcttctat taatttaatt tctagctgac ttatcatata ttcatctaca taggtaacga
      121 attgacctac ctcgttaaat ccatttgtca aacataactt agccatataa gaatgataat
      181 gtttgagtat catcttgatc gaaattggat ctccttctag agcagataag ataatggata
      241 gtggaactaa taggtactgc cgctttttag atttatctac cataggtcat accccatttt
      301 caattgttca actgccttct ttcgttgata ccagatacca cttgtgctca tattgagaat
      361 tttaccgatt tcagcatcat taaaacctgc aaaataataa agtaggataa tctttcttct
      421 tatctccgat aactgatcaa ttgctgctga taacttttga tctgaaatta atatttccat
      481 tccaaataag aaatagacat cttgttcatc aaaatgagtt tgggaatacc tgccttcatt
      541 tgaatctgcc aatggttcct cattatcctt tatgttagcg tttcgttttc gaatagactt
      601 agcttcgttt cgtaatactt ttttacagta ggcatcaaat tggtgttgta tagatatgtc
      661 attgtaatgt tgttgcatga taatatgctc ctttttcccc ctttccacta atacaacaat
      721 cagaaagccc ctttttttcg aaaaaactag aaaaaaaaca taaaaatatt tatcattctt
      781 taaacagaaa atctttctat aaaaaacaa
//
LOCUS       DADVXU010000258          794 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.266, whole genome shotgun sequence.
ACCESSION   DADVXU010000258 DADVXU010000000
VERSION     DADVXU010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.266"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..767)
                     /locus_tag="KB953_002994"
     CDS             complement(<1..767)
                     /locus_tag="KB953_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867877.1"
                     /translation="MLFLSFAYVIPLFMSKGIYHSVNQDTYFHLSRIIGLDNVWSSPV
                     NFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFITFLTMVVSYFSMK
                     QIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYIRDYQK
                     WYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVLTFFLS
                     AGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHIL"
BASE COUNT          317 a          121 c          130 g          226 t
ORIGIN      
        1 tttaaaatat gtgtcagcat atcactagga gccataccac ttaattcttt tcccagtggg
       61 actttcaatt cttgtgctct agattgttga ataaaaggaa ttaagaatcc cgcacttaaa
      121 aaaaaggtga ggacggtagc ttttaaaaga cttaagagtc tagcgatttt ttggtcccaa
      181 aaataaaaag aaagaaacaa cgttcctcca ataaatacag aaaccatagc gacagaaagt
      241 aaatgagtat acactactag cgtcatgcca atagacaacc agtaccattt ttggtaatct
      301 ctaatataaa tttcgtaaca ccccattagt atcaaaggca aaaatgttaa agcgacagct
      361 tctcccaatg aagcacgatg gaatatgtca atggcacgat aggcggaaaa tgtataaata
      421 atcgaaaata acagcgaaat ataacgattg ttttttattt gtttcataga aaaataagaa
      481 acgaccattg ttaaaaaagt gataaaaaaa tagtagatat tataacttaa aacaagattt
      541 cccatcattt tataaaataa aaaagctgga taaagagtaa gccatggata aaaaatattc
      601 atcattgttc catgatgatc gaagttattg aagtttactg gagaactcca tacattatct
      661 agtcctatga ttcgagataa atgaaagtaa gtatcctgat tgacactatg atagattccc
      721 ttagacatga atagagggat gacgtaagca aaacttagaa ataacataaa aatgatatct
      781 attatttggt tttt
//
LOCUS       DADVXU010000259          791 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.267, whole genome shotgun sequence.
ACCESSION   DADVXU010000259 DADVXU010000000
VERSION     DADVXU010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.267"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            12..>791
                     /locus_tag="KB953_002995"
     CDS             12..>791
                     /locus_tag="KB953_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867878.1"
                     /translation="MEIFGFYDTAFHINRALSLESIFSSPINFETFRSYGMQVNNFYP
                     WLTLYPLFLLIKFTNLAIGYNLFLYIVTLITLFICHYVMYEITKKHVTSSFFAIIYTT
                     SSFRSVEIFLRGAMGELLAMSILPLILLGFIKLYDSKKESWVMLAISMTLLIYTHVLS
                     VAMTMVMIIIALIIQFYRKKKIEIFLIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLN
                     RPYVDILQKRAIYLGKEFLIGSIDSELLSFGIGLTLLICLII"
BASE COUNT          247 a          110 c          117 g          317 t
ORIGIN      
        1 catttaaaaa catggaaata tttggctttt atgatacggc atttcacatt aatcgtgcgt
       61 taagtttaga atctattttt agctccccta ttaattttga aacgtttaga tcatatggca
      121 tgcaagtaaa taatttttac ccatggttaa ctctatatcc tttattttta ttgatcaagt
      181 ttactaattt agcaataggc tataaccttt ttctttatat tgttacgctc attactctat
      241 ttatatgtca ttatgtaatg tatgaaatta ccaaaaaaca tgttactagc tcctttttcg
      301 ctattatata tacaacatct tcttttagga gtgtagaaat atttcttcga ggggcaatgg
      361 gggaattact cgcaatgtca atacttcctc ttattttact cgggtttata aagctttacg
      421 actcaaaaaa agaatcttgg gttatgttgg cgattagcat gactttatta atttacactc
      481 atgttctttc tgtagctatg actatggtaa tgattataat tgctttaatt attcaatttt
      541 atcggaaaaa gaaaatagaa atcttcttaa ttattaaatt aataaaagct gctatagtta
      601 ctttacttct ttcactagcg tttataggtc ctatgattga gcagacattg tatcaagatc
      661 tgaatagacc ttatgtggat atattacaaa aaagagcaat ttatctgggt aaagagtttt
      721 tgattggaag tatagattca gaattactat catttggtat tggcttaact ttgcttattt
      781 gtttaattat a
//
LOCUS       DADVXU010000260          789 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.268, whole genome shotgun sequence.
ACCESSION   DADVXU010000260 DADVXU010000000
VERSION     DADVXU010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.268"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..632
                     /locus_tag="KB953_002996"
     CDS             <1..632
                     /locus_tag="KB953_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HBB6867879.1"
                     /translation="NGDIRNLENMVFNLSNGIIPSVSGDTIRSEKNYSIIVFEKLAQT
                     SITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPLI
                     SSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMHITIYNYISKRKISTAKIM
                     LKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
BASE COUNT          281 a           90 c          147 g          271 t
ORIGIN      
        1 aaaatggaga tattcgaaat ttagaaaaca tggtttttaa tctgagtaat ggaataattc
       61 caagtgttag tggagatacg attcggtctg aaaaaaatta ttctatcatt gtttttgaaa
      121 agttagcaca aacgtctata acattaggaa tggatataat agaagcgtat caaagtagag
      181 atgctttgat acaagaaaac gaattagctg tatcgttacc tgaggtctta aaagtgaggg
      241 attctgggat tgtttattat actaaggaaa tcggaaaaac taagatagag catctttctc
      301 ccttaatttc ttctgtcgtt caattcatcg gcttaaatat ctataaaaga attactgtaa
      361 aggaaatagc taattatttt tctgtaagtg aaactaaatt aagagaaagc tttaagaatg
      421 aaatgcatat tacgatttat aattatatct ctaagcgaaa aatttctacg gctaaaataa
      481 tgttgaaatc aaaccatact atcagtgaag tttctttagg tttaggattt tcagattctt
      541 cacacttttc gagagtattc aaaaagtacg caggtgtatc tcctaagcaa tatcaattag
      601 gtttagtgga caatttcaac aacattagct gagaggtttt attttattat gagactatat
      661 catttacgta gtttggagga gttgaggaaa ttggatttta acaaaaaatt tttttgggaa
      721 atttctgatt tgagttgttt cgtagaatct gagcgtctaa gtaatggtaa taatagtgtg
      781 ttaaatgaa
//
LOCUS       DADVXU010000261          780 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.269, whole genome shotgun sequence.
ACCESSION   DADVXU010000261 DADVXU010000000
VERSION     DADVXU010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.269"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..279)
                     /locus_tag="KB953_002997"
     CDS             complement(<1..279)
                     /locus_tag="KB953_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase domain-containing protein"
                     /protein_id="HBB6867880.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIP"
     gene            complement(272..>780)
                     /locus_tag="KB953_002998"
     CDS             complement(272..>780)
                     /locus_tag="KB953_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBB6867881.1"
                     /translation="TTAFKIFRAIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKEC
                     LTELIQMYEQNQQDYLNFKIGMFAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIE
                     ETTLQTSKLANIHIIDPSMNKWFDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEI
                     LKVGNIDE"
BASE COUNT          276 a          122 c           92 g          290 t
ORIGIN      
        1 tggaattaaa aaattttctc tgttaatgat tatagttaag ataaaactaa tcattgctcc
       61 aatcagtagc caagaaaaac gcattttaaa aaaatttttc tttataaaaa cctcatacaa
      121 aactataatt ccaccccaag caaaagctat tttcatcggg atattagtta cattaaaagt
      181 actaggcata aaataaagca gacaaatgat taaatttatt actcgaaacg catctcgact
      241 taataaactc ttctctaaaa caggtactat tctattcatc tatatttcca actttcaaaa
      301 tttctttatc aattctattt aatagtttat cagagtaatc cgatttaact tttcttacaa
      361 ataatttttc actttctttt atttgatcta tatccctttc atcaaaccat ttgttcatgg
      421 aaggatcaat aatatgaata tttgctaatt tcgaagtttg taaagtagtt tcttcaatat
      481 ttttcaattc aagtggtaaa tctggtcctc caataacatt tttatttttg aacgaggaat
      541 taaaaaaaag tgtatgaaaa aatttttcat ctggggcaaa catacctatt ttaaaattca
      601 ggtagtcttg ttgattttgt tcatacattt gtattaattc tgttaggcat tctttcgaca
      661 aagcccacca ttgagaacca tgatacaaat caaatttatg gtgtctaatt tttgggggaa
      721 gtttaatcat cctcagacaa cgattaatta tagctctaaa aattttaaat gcagttgtgt
//
LOCUS       DADVXU010000262          760 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.270, whole genome shotgun sequence.
ACCESSION   DADVXU010000262 DADVXU010000000
VERSION     DADVXU010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.270"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            22..252
                     /locus_tag="KB953_002999"
     CDS             22..252
                     /locus_tag="KB953_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBB6867882.1"
                     /translation="MGFRTKRSLSSKKNWVYSQTIFYGGIVLISLLSSTLYSLNIIDV
                     STSNSISIIGIIIAAIITQLFLVFGEKKRSKK"
     gene            complement(343..708)
                     /locus_tag="KB953_003000"
     CDS             complement(343..708)
                     /locus_tag="KB953_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867883.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
BASE COUNT          287 a           98 c          136 g          239 t
ORIGIN      
        1 gataggagta gaaattcaag tataggtttt agaactaaac gaagtttgtc ttctaaaaaa
       61 aattgggtat actcacaaac tattttttat ggaggaattg ttttaattag tttactttct
      121 tcaacacttt attctttgaa tattatagat gtttccacct caaattcaat atctattatt
      181 ggaatcatta ttgctgcaat tattacacaa ctgtttttag tgtttggaga gaagaaaaga
      241 agtaaaaaat agaaaaacta gtgatagttt tggtgatata agaaaaacat atatgtataa
      301 aaagaagaaa gtagcttctc tttgtacata tatggtttgt ttttatggat gccgagcttt
      361 tttataagat aaggctaccg cccataacga cccaaggatt tgtggaacta agtaaatacc
      421 tgcaagaagc aagatcagct gggtcggttt taaaggatga tagaagtaac gccattcagt
      481 aaaattggcg caaaataaat aggaagtgac agtagatata atattggcaa tgaataaaga
      541 tataatacgc ctgttccttt gaaaataata tccagcaaaa agagataaaa atatcaatgt
      601 gatgaacccg cttacggaat gataacgaaa atcaagcagt aaggcgaaat gcacataagg
      661 aacaaaagat aaaagcaacg gaaggttttt ccagcaagtt tttatcatct ttcatcacca
      721 cctaacaagt gttattattt tgttagtttt attgtaaatt
//
LOCUS       DADVXU010000263          711 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.271, whole genome shotgun sequence.
ACCESSION   DADVXU010000263 DADVXU010000000
VERSION     DADVXU010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.271"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..125)
                     /locus_tag="KB953_003001"
     CDS             complement(<1..125)
                     /locus_tag="KB953_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HBB6867884.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYG"
     gene            complement(143..463)
                     /locus_tag="KB953_003002"
     CDS             complement(143..463)
                     /locus_tag="KB953_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6867885.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
BASE COUNT          243 a          131 c           86 g          251 t
ORIGIN      
        1 ccgtatagat tttcattcaa aaaaatgatt ctgggttttg gatagtagaa accaggctct
       61 ttcatctcca tataaactac tttcaaattg tattcgctca acatttcttt caatttcaaa
      121 tacatacaaa ccatcactcc aactattcat tttcctctaa agctttagca attgcaatcg
      181 ctttacgcat tgtctcctta gatatttctt ttccatcaaa agaaaaaaca gtatcgtctt
      241 ctgataaatc cacatgttta ggggtttctc tttcttctct acctagaagg tagtctacag
      301 agacatcgaa atagtcagca atccgactta attcatctga attaggagta ttatttttcc
      361 actttgctag ataaccgtta gaatatccaa aattaatttc taactgccgt atagatatct
      421 ttttcctctt cgccaagtct tttattattt cataggtatt catcgataaa acaacctttc
      481 taaatgttta caaaaaaagt ttagaaaaat acgcagaaat aatttgacta attctgagta
      541 atgagctata ctatatcttg taaacagatt taacaactaa aaagacaaca aaaaacacta
      601 ttgatatgta aatgcagacc gccaagaaag ctttaaaatc aatgttttta tgtcttattt
      661 aattatgctc ttagtataga gtattactca gatactgtca attgaattta g
//
LOCUS       DADVXU010000264          652 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.272, whole genome shotgun sequence.
ACCESSION   DADVXU010000264 DADVXU010000000
VERSION     DADVXU010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.272"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..611)
                     /locus_tag="KB953_003003"
     CDS             complement(<1..611)
                     /locus_tag="KB953_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867886.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQ"
BASE COUNT          282 a           94 c          122 g          154 t
ORIGIN      
        1 ttttgtttgt tttttctttg ccacataaat acacctagac tgacgatgaa gagcatatcc
       61 aacagatttc ctgcgacttg gatcgtcgaa gggttggtca gtggcattaa agcaaggaca
      121 aaaaaggaaa acaagctgta aataaaacag gtgacaggaa ctaatcgaaa gaagagcgga
      181 catttcaatt ggctaaaaat gagtgaaaaa ataaaataag taaaaatgga taaaatcacg
      241 actaaaccat tcatgataaa gaagaaaagc gtattgtgat taaacgaata aaaaagaatt
      301 gctaaaaaag aaaacgtttt aaatgcaatc aatacgcttg aagcttcgtt taacgtacca
      361 ggatgtcgat gaaaaaaata ggcaccgaaa taaacagaaa acagtaagcc gcaaagagaa
      421 gacaaaatca aaaaaaacgg attttcttcg gcttctgtga tatcagaatg atccaaaagc
      481 aaccagccta atgcactaga aggcgcatgt gaggtcttaa ggtatgaata acatagaaaa
      541 aataagatcg tcaaagttgc aaaaaaagag cctaaatgta aaaaattagt ggttctacgt
      601 tgaatttcca taatatctcc aaattaaatt gataagtata taaagtataa ta
//
LOCUS       DADVXU010000265          640 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.273, whole genome shotgun sequence.
ACCESSION   DADVXU010000265 DADVXU010000000
VERSION     DADVXU010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.273"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..447)
                     /locus_tag="KB953_003004"
     CDS             complement(<1..447)
                     /locus_tag="KB953_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6867887.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT          189 a          116 c           88 g          247 t
ORIGIN      
        1 caactttctc ttttcaaaaa aaatttgata tttttttaga tttcttttta gtctttgtaa
       61 tactttataa gccacagaat aactcacgtg atgctgttta gcaaaatttt ctactgagat
      121 atatttttcc tgaaagaaag ccaataccat tttcatagat aaagaatccg ttatataacg
      181 agataacaga gctttgctag aacattttcc attaatttcc agacatatat ccaattcact
      241 gacggttact cgcaaatttt tctctagatc gaacttttgt aaatcatgtt tcagcttatc
      301 gatcgttttt ttcaagagaa atgtcgatag cgacaactct tcttgaacat tcgatattga
      361 ctctgataaa tttgagcttg cttctaaata tttaagtaat gaataacttc tgtgatcttc
      421 tttgcttaaa aaaaactcat aattcattag tcatcagctc tttctacgta tattactttc
      481 catgcattca ccaactagct ggggataaaa ctgatttcct ggatccgagt cactgttgtc
      541 agcgattctt gtttgtataa tatacatcgt cctgtctttt ttttaacatt ttggagagcg
      601 ttttatttgg cctttctcgc gatcaaacct gttattagaa
//
LOCUS       DADVXU010000266          625 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.274, whole genome shotgun sequence.
ACCESSION   DADVXU010000266 DADVXU010000000
VERSION     DADVXU010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.274"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            1..625
                     /locus_tag="KB953_003005"
                     /pseudo
     CDS             1..625
                     /locus_tag="KB953_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          247 a          105 c          118 g          155 t
ORIGIN      
        1 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
       61 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      121 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      181 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
      241 caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt cgctgaagaa tttatccgca
      301 gccgaaaggc ctacggctca agaaaaataa aaaaagcctt atcaaaacga ggcattcaga
      361 tcagccgacg aaaaattagt agaatcatga aaaatagagg attaaaatcg agctatactg
      421 ttgcttattt taaagtacat cattctactt gcaatgaagc caaaacgaca aacgtattga
      481 atcgtaaatt cttaagagac aacccattag aagcgatcgt aacagacttg acttatgtac
      541 gagtcgggaa aaaatggaat tatgtctgtt tcattttgga tctgttcaat cgagaaattc
      601 tcggctattc ttgtggagaa cataa
//
LOCUS       DADVXU010000267          534 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.275, whole genome shotgun sequence.
ACCESSION   DADVXU010000267 DADVXU010000000
VERSION     DADVXU010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.275"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..>534
                     /locus_tag="KB953_003006"
     CDS             <1..>534
                     /locus_tag="KB953_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HBB6867888.1"
                     /translation="DGFKACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDL
                     KPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNT
                     SAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINWARGGQVGSWFADGTYGHTGVIR
                     GLANGRMQTYEQNTELG"
BASE COUNT          163 a           80 c          137 g          154 t
ORIGIN      
        1 tgatggattt aaggcctgta ctgatccaag acaagcagca tatgcttttg aacgaaacta
       61 tgaacgtccg gcagcgacac atcctgaacg tcaagattat gcagttaact ggtataacaa
      121 atttaaagat ttaaaaccag gaggagctac tggagaagca ggactaaaac atttagagtc
      181 tttgattgga caacgaattg gcaatggtca gtgttatggc ttgtctgcag aatattcagg
      241 atatctgggt ggttgtggca tgggtgctgg aacaaaatat ggtttaactc atgtgatagg
      301 aaatacttct gcagcatctg atattggtat tgcctatgat tggtctgctg ttggttggaa
      361 agtgattcaa aaccctagat atgatcaatt agtagttggt gcaattatta attgggcaag
      421 aggtggacaa gtgggttcat ggtttgcaga tggaacttat ggacatactg gcgttatccg
      481 aggcttagct aatggtcgta tgcaaactta tgaacaaaac acagagttag gtat
//
LOCUS       DADVXU010000268          519 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.276, whole genome shotgun sequence.
ACCESSION   DADVXU010000268 DADVXU010000000
VERSION     DADVXU010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.276"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..221
                     /locus_tag="KB953_003007"
     CDS             <1..221
                     /locus_tag="KB953_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBB6867889.1"
                     /translation="KFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKY
                     FFENVVTGKENWNDLYHANEVLKIAEEK"
     gene            222..>519
                     /locus_tag="KB953_003008"
     CDS             222..>519
                     /locus_tag="KB953_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HBB6867890.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLE"
BASE COUNT          202 a           59 c          100 g          158 t
ORIGIN      
        1 gaaaatttca attgtttact ttagatgaat gtatcaatgt tgacttatta aataataaaa
       61 taagttttga agaaaacatt gttaaagaaa tgcctgaatc tgctaatgat atgtatgaga
      121 aggagttgaa atatttcttt gaaaacgtag taacaggcaa agaaaactgg aatgatttat
      181 atcatgcaaa cgaagtattg aaaatagctg aggagaaata aatggaaatt ttatttacta
      241 tttgtggtag agctgggtct aaggggttta aaaataaaaa tttgaaatat ttggcaggaa
      301 agcccctagt ccatttcgct ttagcaatga tagatatcta tcaacaaaaa catcctgaac
      361 ataattgcac gacttgtttg aatacggaca gtaatgagtt agtagaattg acgactaagt
      421 taaatccgtc tgtgaaaatt gtagaacgta aacctgaatt agctacagat actgtaggga
      481 aaattgaagt gatacgtgat tcattcttac aactagaaa
//
LOCUS       DADVXU010000269          519 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.277, whole genome shotgun sequence.
ACCESSION   DADVXU010000269 DADVXU010000000
VERSION     DADVXU010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.277"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            141..398
                     /locus_tag="KB953_003009"
     CDS             141..398
                     /locus_tag="KB953_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867891.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
BASE COUNT          218 a           65 c           73 g          163 t
ORIGIN      
        1 ttctgtccac gttcatttta ctccaaagtt ttcctcattt caaaagaaaa tcacagaata
       61 cacgaagaag cgtttctatt tgtataaact atatcaaagt tctatactta aattatcaaa
      121 taaaaaggac gtgaagataa atgtcaaatt tagaaaacaa agaagaaaaa gttgtaaaca
      181 aaattgtttc tgttgtcaat aaactggata aagagttaga cgaacttgat acgttatctg
      241 aaaatccaga aaaaaaacat aacctaaaaa aatggttagt tgaacgaaag gctattcatg
      301 aaattaaaaa agttcttcat gaagctgata aatatgaaaa atatgatgaa aaagaacttg
      361 ataaagaatt taaagaaatc aatgacttat tactataatt gatttatacg actcattttt
      421 gagtcgtcag actgcaaaca acactctaaa tttaggagtt tgtctacagt ctgaatagat
      481 tttaaattta ttgtccataa tgttattatg aatttatag
//
LOCUS       DADVXU010000270          516 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.278, whole genome shotgun sequence.
ACCESSION   DADVXU010000270 DADVXU010000000
VERSION     DADVXU010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.278"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(107..>516)
                     /locus_tag="KB953_003010"
     CDS             complement(107..>516)
                     /locus_tag="KB953_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HBB6867892.1"
                     /translation="GTPENHFSNQISLNVYSRGMGGLGLPGDLSSVSRFVKATFTKMN
                     AASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSCCNVDKGIYYYRTYED
                     SQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT          163 a          103 c           78 g          172 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttccat
//
LOCUS       DADVXU010000271          495 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.279, whole genome shotgun sequence.
ACCESSION   DADVXU010000271 DADVXU010000000
VERSION     DADVXU010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.279"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
BASE COUNT          167 a           96 c           58 g          174 t
ORIGIN      
        1 aaataagaat gctaaatcac tctacaataa cggcgaaagc acaaaaaata aaaaaattat
       61 tacgttatta aaaaaacaga tgaaagatca ggttttatta gatctcattc tttcatcact
      121 tgaagggatt taatgaacta ctttttcctc tttgtcgttt ttatatattc gtattccttc
      181 aaacagtagg atagcctctt tcaatgcttg atatttgaac tgtccgttac ttaaaaattt
      241 agatgtgccg ccctcttcct agaacatatt cttttttact ttttcattaa ttgacagacc
      301 aatcgaacga aactgtaaat ttcgatagga taatgattgg tcaaactcat tcattttcaa
      361 gacatctcga ttcatattca agataggaat ctttttatca acatcacttt attcgtattt
      421 tcctctacca taaaagcttc acgctccaca aaattgtttt atacactact attcagccca
      481 ttcaacgacc aatga
//
LOCUS       DADVXU010000272          487 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.280, whole genome shotgun sequence.
ACCESSION   DADVXU010000272 DADVXU010000000
VERSION     DADVXU010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.280"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..>487)
                     /locus_tag="KB953_003011"
     CDS             complement(<1..>487)
                     /locus_tag="KB953_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HBB6867893.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IK"
BASE COUNT          142 a           92 c           86 g          167 t
ORIGIN      
        1 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
       61 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
      121 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
      181 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
      241 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
      301 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
      361 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
      421 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg tagaaacgac
      481 gatggat
//
LOCUS       DADVXU010000273          464 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.281, whole genome shotgun sequence.
ACCESSION   DADVXU010000273 DADVXU010000000
VERSION     DADVXU010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.281"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..>464
                     /locus_tag="KB953_003012"
     CDS             <1..>464
                     /locus_tag="KB953_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBB6867894.1"
                     /translation="LVTKGDLNELFRSHLEKAINTLLRTELTAFLDYEKYDRTGFNSG
                     NSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLETTIIHLFEKGVT
                     MSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAIFMDAT"
BASE COUNT          168 a           85 c           77 g          134 t
ORIGIN      
        1 ctagtcacta aaggcgattt aaatgaatta ttccgttcgc acttagaaaa agcgataaac
       61 acactcctac ggactgaatt aacggctttt ttagattacg aaaaatatga tcgcactggt
      121 tttaattcag gtaattcgag aaacggttct tactttcgat caatcaaaac cgaatatggt
      181 gaattaacat tggaaatacc tagagatcgt aatggtgagt ttaaacaaca aactttacca
      241 gcctacaaaa gaacaaacga tacattggaa accactatta tccatttatt cgaaaaaggt
      301 gttacgatgt ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca ctatactcca
      361 caaaccatgt ccaacatgac taaagttctg actgaagaag taaatgcctt taaatccaga
      421 gccttaaatg ataagtatgt cgctattttt atggacgcta ctta
//
LOCUS       DADVXU010000274          444 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.282, whole genome shotgun sequence.
ACCESSION   DADVXU010000274 DADVXU010000000
VERSION     DADVXU010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.282"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
BASE COUNT          161 a           63 c           54 g          166 t
ORIGIN      
        1 gtaattccta attttgctcc acttttagat aatttttagg tctatttaac tcatcagaca
       61 ttctatgctc atttttctcc aaatttgttt caactctttc gtacttaatt ttgcgtattt
      121 gaaaattctt cttttagagt acttttatta cgatactagt atgatatata tctatagcaa
      181 acagttaaaa tcttcctaaa ataagcgaat agcattccta aaacctataa aatttttaga
      241 tgaagcgaca agtggacaat taataaacta cttgaacatg aaattatgga tcacctttca
      301 aatttagaaa atggaattct tatacttatt gcgcaacatt tattgattgc aaaattatgt
      361 gacaaaaaaa actagtagaa gaaagaataa tggcttgata ataaagtact acctattaaa
      421 ggtagtactt tatttaattt agta
//
LOCUS       DADVXU010000275          441 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.283, whole genome shotgun sequence.
ACCESSION   DADVXU010000275 DADVXU010000000
VERSION     DADVXU010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.283"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(124..>441)
                     /locus_tag="KB953_003013"
     CDS             complement(124..>441)
                     /locus_tag="KB953_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBB6867895.1"
                     /translation="ANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYS
                     TNLIEGFNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLE
                     SMF"
BASE COUNT          142 a           60 c           69 g          170 t
ORIGIN      
        1 acattttttt ataaaaaata accacccgaa ggtggctgaa gaataagctt ttatttgtaa
       61 gtttccagaa taggttgtgt caataattat gtgtaaatgg aaaagcccat tctgttgatc
      121 aagttaaaac attgattcta atgtatcatg tatttcttta aaacctttat gaactcggca
      181 tagaaacttt tgattatagt tgttgaattg agatactaga aatctctcca gagattcttc
      241 attaggaaat tgctcttttc ttttggtata tttctttaat tgtttgttaa aaccttcaat
      301 caaattagtt gaataaatag ttcttctgat tgacggaggg aaattataaa aggttaatat
      361 agcaggattc atgagcaatt taaccactcg tggatactgc tttttccatt tatctatcat
      421 aaaccttatt tgattatttg c
//
LOCUS       DADVXU010000276          439 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.284, whole genome shotgun sequence.
ACCESSION   DADVXU010000276 DADVXU010000000
VERSION     DADVXU010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.284"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..>439
                     /locus_tag="KB953_003014"
     CDS             <1..>439
                     /locus_tag="KB953_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867896.1"
                     /translation="TVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPITADDTE
                     FVSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDV
                     SAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          177 a           67 c          101 g           94 t
ORIGIN      
        1 caacagtctt tgcagtgaaa gacggcgtaa gcttaccaga agataaactt ccagtgttga
       61 aagcaaaaga tggttataca gatgcgaaat ggccagagga agcaactcaa ccaattacag
      121 cagatgatac agaatttgta tcaagtgcaa caaaactgga tgataaatct gacgctgaca
      181 aatataatcc tgaaggtcaa aaagtgacta cagaattgaa taaggaacca gacgcatttg
      241 agggaattaa gaacaaagaa gatttaccaa aagatactaa gtatacttgg aaagaaaaag
      301 tagatgttag cgcagctgga aataaaaaag ggactgttgt agtgacatat ccagatggat
      361 catctgatga agttgaagta gatgttacag tgacagacaa tcgttctgac gccgataaat
      421 atgagccaac agtagaagg
//
LOCUS       DADVXU010000277          430 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.286, whole genome shotgun sequence.
ACCESSION   DADVXU010000277 DADVXU010000000
VERSION     DADVXU010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.286"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            79..>430
                     /locus_tag="KB953_003015"
     CDS             79..>430
                     /locus_tag="KB953_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6867897.1"
                     /translation="MKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKD
                     IQERYSDTFSLDKDY"
BASE COUNT          177 a           67 c           66 g          120 t
ORIGIN      
        1 ataaaaagta aatactttta aatactttta ttttataaat atgatatact atctttaaaa
       61 ataaaaagga gaaaaaagat gaagccaaat ccttcagaag caggaaaacg aatcaaacaa
      121 ttacgccttt catgtggttt tactatggaa gaacttggaa ggaaaataga taattcacca
      181 cgtgcgacca tatccaactg ggaacgtggg accaatttac caaatccaca aaagctgaaa
      241 ttattatcaa ctatcactaa ttcaacaatt gactggatta aatggggaac tttagaagaa
      301 tatataactt cataccttat cgatataggg tatgagctat atattaagga cttcccagaa
      361 atacctcaca aagtctttaa agacattcaa gaaagatata gtgatacgtt ttcgctagat
      421 aaagactatg
//
LOCUS       DADVXU010000278          421 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.287, whole genome shotgun sequence.
ACCESSION   DADVXU010000278 DADVXU010000000
VERSION     DADVXU010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.287"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..390
                     /locus_tag="KB953_003016"
     CDS             <1..390
                     /locus_tag="KB953_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBB6867898.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          163 a           74 c           65 g          119 t
ORIGIN      
        1 aaagaccgaa aagaaatctg tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa
       61 gaagcgaata ccttcttatc cggcatgatt gagaaatgga agaaaaacta tcctaaagtg
      121 acgcagtcac tcatagaaaa ccaagactta ttaacttttt atgattttcc acctagcatt
      181 cgtagaacca tttactcaac caatctaatc gagtctttca ataagcaaat taaaagatac
      241 agccgtagaa aagagcagtt tcaaaatgaa gaatcactag aacgctttct agtcagcatt
      301 tttgatacat acaatcaaaa atttctaaac agaagccata aaggttttca acaggtaacc
      361 gatacattag tttcaatgtt tactgagtaa ctaattattt tgcaggagga caatttattt
      421 a
//
LOCUS       DADVXU010000279          415 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.289, whole genome shotgun sequence.
ACCESSION   DADVXU010000279 DADVXU010000000
VERSION     DADVXU010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.289"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..405)
                     /locus_tag="KB953_003017"
     CDS             complement(<1..405)
                     /locus_tag="KB953_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBB6867899.1"
                     /translation="MVPCYNVEKYIDECINSLINQTLKDIEIILLNDGSTDNTLAILK
                     KYEKLDSRIKVYEHKNMGLGPTRNRGIKIANGEFLAFVDSDDFVSNTMFESLYNCAIA
                     ENADIAIGEVGIFSDREIERVKIRKSLTNLKTI"
BASE COUNT          134 a           84 c           35 g          162 t
ORIGIN      
        1 aattgttttt aagttcgtta gactttttct tatttttact ctttctattt ctctatcaga
       61 aaaaatgcct acttctccaa ttgcaatgtc cgcattctct gcaattgcac aattatataa
      121 actttcaaac atagtattgg aaacaaaatc atcagaatcc acaaaagcca aaaattctcc
      181 attagctatt ttgatccctc gatttctagt tggccctaac cccatatttt tatgttcgta
      241 aacttttatt ctagaatcaa gcttttcata tttctttaat atcgccaacg tattatctgt
      301 tgaaccatcg tttaacaaaa taatctcaat atctttcaat gtttgattta tcaaactatt
      361 tatacactca tcaatatatt tttcaacatt ataacaaggt acaattacac taatc
//
LOCUS       DADVXU010000280          415 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.290, whole genome shotgun sequence.
ACCESSION   DADVXU010000280 DADVXU010000000
VERSION     DADVXU010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.290"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..339)
                     /locus_tag="KB953_003018"
     CDS             complement(<1..339)
                     /locus_tag="KB953_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6867900.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DADVXU010000281          396 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.291, whole genome shotgun sequence.
ACCESSION   DADVXU010000281 DADVXU010000000
VERSION     DADVXU010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.291"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(91..>396)
                     /locus_tag="KB953_003019"
     CDS             complement(91..>396)
                     /locus_tag="KB953_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBB6867901.1"
                     /translation="TIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQF
                     ETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          107 a           95 c           78 g          116 t
ORIGIN      
        1 ttgtgaatgc tgcggtgtta tggctacgat tgcccttgac aatgagcatc ctcggatctg
       61 attttctccg gcagaagcac aatcattcaa ttacgctgcc tctccagagg tatcagatcc
      121 gcgctcatta tcaaggattc gcttcgccaa tctctgttgt cgaatttgga tcggtgtttc
      181 atacgccaac gtgccatgta agcgtagata gttccaccaa tgcacataat caaacaattc
      241 tagacgtagc tgtgccagtg tctcaaattg gtattgatgc acgaattcta ctttgacaga
      301 tttataggtt gattccacaa cggcattatc atacggacaa cccttcctac tcaatgaacg
      361 agtaattcca aaaccattca gaatctcatg aatggt
//
LOCUS       DADVXU010000282          386 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.292, whole genome shotgun sequence.
ACCESSION   DADVXU010000282 DADVXU010000000
VERSION     DADVXU010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.292"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..>386
                     /locus_tag="KB953_003020"
     CDS             <1..>386
                     /locus_tag="KB953_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBB6867902.1"
                     /translation="KTFTGMIFKFANRRYGLKYNPVNGVSLARKPKTNEEIKKEKDGY
                     LSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGELISLTESDYDGNKITVSGTY
                     DYELKIKTTTKNTGSYRTIELSSNAK"
BASE COUNT          158 a           48 c           59 g          121 t
ORIGIN      
        1 aaaaactttt acaggaatga tttttaaatt cgccaaccgt cgatatggat taaaatataa
       61 tcccgtaaat ggggtttctt tagcaagaaa acctaagaca aacgaagaaa taaaaaaaga
      121 aaaagatggt tatttatcaa aagaagaagt tcaaatgatt gctagtaaac aaaaaagttc
      181 acatcaacag tcacgttatt ctttaattac taaatttttg tttttaactg gactacgtta
      241 tggtgaattg atatctttga ctgaatctga ttatgatggt aataaaatta cggtttctgg
      301 tacttatgat tacgagttaa aaattaaaac aacaactaaa aatacaggtt cttatagaac
      361 cattgaatta tcaagtaatg ctaaag
//
LOCUS       DADVXU010000283          370 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.293, whole genome shotgun sequence.
ACCESSION   DADVXU010000283 DADVXU010000000
VERSION     DADVXU010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.293"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            <1..>370
                     /locus_tag="KB953_003021"
     CDS             <1..>370
                     /locus_tag="KB953_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867903.1"
                     /translation="VKEMNFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQV
                     TEDGQVIYSSFSPNKDEITFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPF
                     VTVKSESGINKVTIRYSYTFF"
BASE COUNT          151 a           44 c           59 g          116 t
ORIGIN      
        1 ggtgaaagaa atgaattttc aaggaactta ttctaatgaa caaatggaaa ataaacttga
       61 agaatttgag aattttgatt atgtacctat aggctataaa aaagatcaat tgcttttgaa
      121 aaaccatcaa gttacagaag atggtcaagt tatttatagt agcttttctc caaataaaga
      181 tgaaattaca tttacgttta aatcttcaaa aggcaaaaca atagttttac ctgtattccg
      241 gtataaagga ttaacggtta ctgttaattc aaaaaatgct acagtactaa aacaaggagg
      301 tccttttgtt acagtaaaaa gtgaaagtgg aataaataaa gtaacaatca ggtattcata
      361 cacatttttt
//
LOCUS       DADVXU010000284          353 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.294, whole genome shotgun sequence.
ACCESSION   DADVXU010000284 DADVXU010000000
VERSION     DADVXU010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.294"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="KB953_003022"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="KB953_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HBB6867904.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT          114 a           52 c           56 g          131 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttgtatagac tgattgtaac atggaacaaa ata
//
LOCUS       DADVXU010000285          338 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.296, whole genome shotgun sequence.
ACCESSION   DADVXU010000285 DADVXU010000000
VERSION     DADVXU010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.296"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            105..302
                     /locus_tag="KB953_003023"
     CDS             105..302
                     /locus_tag="KB953_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HBB6867905.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
BASE COUNT          122 a           34 c           71 g          111 t
ORIGIN      
        1 tattcaggaa ttattttgct acgtttcaag aaggaattgt tttttgaaat ttaatgaatg
       61 ttagaataat agtctaaaaa cattaattta ggggtgatta gattatgaaa catttaaaaa
      121 ttttgtctat taaagagaca caacttatct atgggggtac cactcatagt ggaaaatatt
      181 atggaaatgg agtgtattgc actaaaaata aatgtacggt cgattgggcc aaggcaacta
      241 cttgtattgc aggaatgtct ataggtggtt ttttaggtgg agcaattcca gggaagtgct
      301 aaaatgaaaa aaaatgctaa gcaaattgtt catgaatt
//
LOCUS       DADVXU010000286          337 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.297, whole genome shotgun sequence.
ACCESSION   DADVXU010000286 DADVXU010000000
VERSION     DADVXU010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.297"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            74..>337
                     /locus_tag="KB953_003024"
     CDS             74..>337
                     /locus_tag="KB953_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBB6867906.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQN"
BASE COUNT          146 a           35 c           54 g          102 t
ORIGIN      
        1 tgtatttgct tcttcattaa tgttaatcaa gaagcctaaa gaaatcttat caaaaataag
       61 ttaggagaaa ataatggata tattagaaat aaagaatgta gcatatagtt atgcaaattc
      121 taaagaaaaa gttttgtcag gagtaaatca aaaatttgaa cttgggaagt tttatgcgat
      181 agtaggaaag tcaggaacag gaaaatctac acttctttcc ttacttgctg gacttgataa
      241 acctcaaaca ggaaaaatat tgtttaagaa tgaagatata caaaataaag gatatagtaa
      301 tcatagaaaa aataatatat ctttagtatt tcaaaac
//
LOCUS       DADVXU010000287          321 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.298, whole genome shotgun sequence.
ACCESSION   DADVXU010000287 DADVXU010000000
VERSION     DADVXU010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.298"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(69..321)
                     /locus_tag="KB953_003025"
                     /pseudo
     CDS             complement(69..321)
                     /locus_tag="KB953_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DADVXU010000288          293 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.denovo.300, whole genome shotgun sequence.
ACCESSION   DADVXU010000288 DADVXU010000000
VERSION     DADVXU010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.denovo.300"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
BASE COUNT           95 a           56 c           34 g          108 t
ORIGIN      
        1 ttagaagcat gtaatttttt ctcagaaaca aaccactcat tggaaaagac atgaatcaaa
       61 tcatcactta cttgtgtaaa atagcgacgt ttaatgacaa agatatcttc attgctatct
      121 agtgtacctt catttctttg tgcttaaatt caaagataat tttctcaata aagatttttg
      181 attccagtaa gattccttta ctccttttac aacatagcgt tgtcctttgt tttgaaacat
      241 cttcttcccc tctttgccat taaaaatagc ccaattctct ataaagaaga tta
//
LOCUS       DADVXU010000289        41032 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.guided.013, whole genome shotgun sequence.
ACCESSION   DADVXU010000289 DADVXU010000000
VERSION     DADVXU010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 41032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.guided.013"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            64..699
                     /locus_tag="KB953_003026"
     CDS             64..699
                     /locus_tag="KB953_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBB6867907.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            748..1428
                     /locus_tag="KB953_003027"
     CDS             748..1428
                     /locus_tag="KB953_003027"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBB6867908.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            1563..2792
                     /gene="rpsA"
                     /locus_tag="KB953_003028"
     CDS             1563..2792
                     /gene="rpsA"
                     /locus_tag="KB953_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HBB6867909.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            2974..4284
                     /gene="der"
                     /locus_tag="KB953_003029"
     CDS             2974..4284
                     /gene="der"
                     /locus_tag="KB953_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBB6867910.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            4527..4802
                     /locus_tag="KB953_003030"
     CDS             4527..4802
                     /locus_tag="KB953_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HBB6867911.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            5037..6083
                     /locus_tag="KB953_003031"
     CDS             5037..6083
                     /locus_tag="KB953_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867912.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            6085..7344
                     /locus_tag="KB953_003032"
     CDS             6085..7344
                     /locus_tag="KB953_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBB6867913.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            7350..7931
                     /locus_tag="KB953_003033"
     CDS             7350..7931
                     /locus_tag="KB953_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HBB6867914.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(8013..8888)
                     /locus_tag="KB953_003034"
     CDS             complement(8013..8888)
                     /locus_tag="KB953_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBB6867915.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            9036..9368
                     /locus_tag="KB953_003035"
     CDS             9036..9368
                     /locus_tag="KB953_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HBB6867916.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            9480..10688
                     /locus_tag="KB953_003036"
     CDS             9480..10688
                     /locus_tag="KB953_003036"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HBB6867917.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            10842..11297
                     /locus_tag="KB953_003037"
     CDS             10842..11297
                     /locus_tag="KB953_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867918.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            11659..11934
                     /locus_tag="KB953_003038"
     CDS             11659..11934
                     /locus_tag="KB953_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867919.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            11947..14025
                     /locus_tag="KB953_003039"
     CDS             11947..14025
                     /locus_tag="KB953_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HBB6867920.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            14185..16071
                     /locus_tag="KB953_003040"
     CDS             14185..16071
                     /locus_tag="KB953_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBB6867921.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            16083..17030
                     /locus_tag="KB953_003041"
     CDS             16083..17030
                     /locus_tag="KB953_003041"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBB6867922.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            17050..17577
                     /locus_tag="KB953_003042"
     CDS             17050..17577
                     /locus_tag="KB953_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBB6867923.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(17640..18293)
                     /locus_tag="KB953_003043"
     CDS             complement(17640..18293)
                     /locus_tag="KB953_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBB6867924.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            18425..19267
                     /locus_tag="KB953_003044"
     CDS             18425..19267
                     /locus_tag="KB953_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBB6867925.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            19425..20321
                     /locus_tag="KB953_003045"
     CDS             19425..20321
                     /locus_tag="KB953_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBB6867926.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            20324..21037
                     /locus_tag="KB953_003046"
     CDS             20324..21037
                     /locus_tag="KB953_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HBB6867927.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            21057..21575
                     /gene="msrA"
                     /locus_tag="KB953_003047"
     CDS             21057..21575
                     /gene="msrA"
                     /locus_tag="KB953_003047"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBB6867928.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            21572..21799
                     /locus_tag="KB953_003048"
     CDS             21572..21799
                     /locus_tag="KB953_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HBB6867929.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            21951..22502
                     /gene="lepB"
                     /locus_tag="KB953_003049"
     CDS             21951..22502
                     /gene="lepB"
                     /locus_tag="KB953_003049"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBB6867930.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(22585..23085)
                     /locus_tag="KB953_003050"
     CDS             complement(22585..23085)
                     /locus_tag="KB953_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HBB6867931.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(23364..23762)
                     /locus_tag="KB953_003051"
     CDS             complement(23364..23762)
                     /locus_tag="KB953_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBB6867932.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            24022..24882
                     /gene="ylqF"
                     /locus_tag="KB953_003052"
     CDS             24022..24882
                     /gene="ylqF"
                     /locus_tag="KB953_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HBB6867933.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            24875..25642
                     /locus_tag="KB953_003053"
     CDS             24875..25642
                     /locus_tag="KB953_003053"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBB6867934.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            25698..26555
                     /gene="dprA"
                     /locus_tag="KB953_003054"
     CDS             25698..26555
                     /gene="dprA"
                     /locus_tag="KB953_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBB6867935.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            26677..28755
                     /gene="topA"
                     /locus_tag="KB953_003055"
     CDS             26677..28755
                     /gene="topA"
                     /locus_tag="KB953_003055"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBB6867936.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            28724..30100
                     /gene="trmFO"
                     /locus_tag="KB953_003056"
     CDS             28724..30100
                     /gene="trmFO"
                     /locus_tag="KB953_003056"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HBB6867937.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            30298..31203
                     /gene="xerC"
                     /locus_tag="KB953_003057"
     CDS             30298..31203
                     /gene="xerC"
                     /locus_tag="KB953_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HBB6867938.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            31240..31788
                     /gene="hslV"
                     /locus_tag="KB953_003058"
     CDS             31240..31788
                     /gene="hslV"
                     /locus_tag="KB953_003058"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HBB6867939.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            31802..33202
                     /gene="hslU"
                     /locus_tag="KB953_003059"
     CDS             31802..33202
                     /gene="hslU"
                     /locus_tag="KB953_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HBB6867940.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            33220..34011
                     /gene="codY"
                     /locus_tag="KB953_003060"
     CDS             33220..34011
                     /gene="codY"
                     /locus_tag="KB953_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HBB6867941.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            34093..34968
                     /locus_tag="KB953_003061"
     CDS             34093..34968
                     /locus_tag="KB953_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBB6867942.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(35100..35738)
                     /gene="plsY"
                     /locus_tag="KB953_003062"
     CDS             complement(35100..35738)
                     /gene="plsY"
                     /locus_tag="KB953_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBB6867943.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            35865..36299
                     /locus_tag="KB953_003063"
     CDS             35865..36299
                     /locus_tag="KB953_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBB6867944.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            36465..38510
                     /gene="parE"
                     /locus_tag="KB953_003064"
     CDS             36465..38510
                     /gene="parE"
                     /locus_tag="KB953_003064"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HBB6867945.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            38522..40972
                     /gene="parC"
                     /locus_tag="KB953_003065"
     CDS             38522..40972
                     /gene="parC"
                     /locus_tag="KB953_003065"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HBB6867946.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
BASE COUNT        14218 a         7043 c         8743 g        11028 t
ORIGIN      
        1 ttttaggaca agacactttt tcctatggta taatacacac gatagagttt aggaggtaaa
       61 acagtgagca gaaaagatag acatcaatct acagaaaata acgaaacaca agaaccatgg
      121 gaacaaccaa tctacgatac agatgatgaa gtttcatcaa gaagttctca gagacaacaa
      181 aagaaaggaa atacattatt tctttcgcta tttttgattt tactggtctt atgtatcgct
      241 attccagcag gtgcttattt ctggatcaga aatggatcaa gcaacaatgc aacagcagca
      301 acttcttctg aaacttcgac atcagtagta gaaagttcta caagtacaac aaaagaggag
      361 tcaactgaga cttcaacagt agaatctagt acaatcgcgg aagaatctac tgtgtctcaa
      421 gatacaggtg taacgtcaga aagcagtatt gcagcagaga caacaccgac ttcctcaagt
      481 gaggcaactt atcaagcagg ggattcaagc agcaatgcag cagaaacaac accttcttca
      541 tcagctgcag ccggttcaac aacgactgtt caagcaggcg aaggtccaaa acaagtggct
      601 gagcgtgcag ggatcaccac tgatcaattg tttgaattga atggcttaga tccaaacaac
      661 tttatgcttt accctggtca agaattaaga attaaataat aatagccgcg ctttgcggct
      721 ttttttctga gaaaaaggag ttatatcatg acacaaatca gtattgcaat cgacgggcct
      781 gcttcatctg ggaaaagtac cgttgccaag atattagcaa aagaattgaa ttatatttat
      841 acagataccg gtgccatgta tcgcgcagta acttatttag ctattacgaa caatgtatct
      901 ttttcagatg aagaagggct agtttcgctg atcaaaaagt atcctatctc tttttcgcaa
      961 caagaagatg gacagcatgt attcatagca tcaaaagatg tgactttaga aatccgtcaa
     1021 ccagatgtca caaaagctgt ttctgaagtc tctgcacatg gaaaagtcag agaagaatta
     1081 gttgctgtcc aacgaaaaat aggggaacgc ggtggcgttg tcatggacgg aagagacatc
     1141 gggacagctg tattaccgaa tgcagaagtg aaaatctttt tagtagcaag cgtaagtgag
     1201 cgtgcagaaa gacgtttcaa agagaaccaa gaaaaaggaa ttccgactga tcttgaaaca
     1261 ctgacgaaag agatacagga aagagaccat tatgattcaa caagggacgt ctctccgctg
     1321 aagcaagcag aggatgctgt tcgtatcgat acgactggca aatcgattcc agaagtcgtt
     1381 gcagcaataa aagctgttgt tttgaaaaaa ggttatcttc ttttttaaat taagaaaaaa
     1441 aatgaaaaac aattgttttt tcaaggaaaa cgaacgaaat cgctttattt tttttgaata
     1501 atcaattaaa ctaaaaacag taccgtactt acggcagaat gttggttagg aggacaagtg
     1561 ttatgacaga atttgatcaa aatcaagaga acaaccagtc aatggaagac gcaatgaaaa
     1621 gcgtacaaga agtaaaagtt ggcgatgttg ttaagggcga agttttagtc attgaagaca
     1681 aacaagtgat tgtcggaatc gaaggggctg gagttgaagg agtcgttccg gcaaaagaat
     1741 tatcaacttt gccggtagaa gatatcagcg aagtagtaaa agtcggtgat gtattagatt
     1801 tagtcgttat cagtacgatc ggcaaagata aagaaaacgg cagctatcta ctttcaaaac
     1861 gccgtttaga tgctaaaaaa gtttgggaag atatcgaaag agatttccaa gagggcaagg
     1921 tcatcgaagc acctgttaca aatgttgtca aaggcggttt agtcgttgac gtcggtgtac
     1981 gtggttttgt ccctgcatca atggtggaag atcattttgt tgcagacttt tcagactata
     2041 aaggcaaaac attgagcttt aaaatcatcg aaatcgaacc atcagaaaat cgtttgatct
     2101 tatcgcataa agcggtagtg gaaaacgaaa gagaagaaaa gaaaaaagaa attctttcaa
     2161 ctatccatga gggagatgtt ctcagtggac gtgttgcacg tcttactgat tttggcgcat
     2221 ttgtcgacct aggcggaatt gacggtctag tccatgtctc agagatttca catagtcatg
     2281 tagcaaaacc aagtgatgta ttggctgtca atgatgatgt aaatgtcaaa gtgttgtcta
     2341 tcaatcctga acaagaacgt gtttccttgt caatcaaaga cacacagcct ggaccttggt
     2401 cagaaatcga tacaaaagct ccagttggca cagtattaga cggaacagtt aaacgtttga
     2461 caagcttcgg tgcctttgtt gaagtgttcc ctggagtaga agggctagtc catatctctc
     2521 aaatttcaca taaacatatt gcgacaccac atgaagtgct tcatgaaggt gacgaagtga
     2581 aagtgaaagt attagagatc catccagaag aacatcgtat tgcattgagt atcaaagctt
     2641 tagaagaaaa accagcttca gaagaagaac caaaaaatgt tgagtcttat gaattaccag
     2701 aagaaaatac tggatttact atgggagaca ttttaggtga tgcgttgaaa gaagaagtag
     2761 aagaaaaagc aaccgatgat tctgaaaaat aattgatttt gtatctgtga ctgagatgaa
     2821 agcagctgct tttgtctcag tttttctttt gaaaagcgat tagtatttca ataaggaata
     2881 aaaatacttc gtttaattga caatctatgc ttgaaagttg gcaatgcatt cgttaaacta
     2941 agtgagattg gaaacttgtg aggaggaaat aatatggcaa atccaacaat tgcaatcgtt
     3001 ggccgcccga acgtcggtaa gtctacgatt tttaaccgca tcgctggtga gcgtatctcg
     3061 attgtcgaag atactcctgg agtaactcgt gatcgtattt atgcaaaagg tgaatggtta
     3121 ggtcgagaat tcagcgtgat cgacacaggc ggaattgatc ttggcgatga accttttatg
     3181 gatcagatca agcatcaagc agaaatcgcg attgaagaag cagatgtaat catttgtgta
     3241 gtcagcggac gcgaaggcgt gacagacgca gatgagatgg ttgcgaaaat tttatacaga
     3301 agtaataaac cagtgatttt agcagtcaat aaagtagata atccagaaat gagaaacgat
     3361 atctatgaat tttattcctt agggctaggc gatccttatc ctgtttcagg aagccatgga
     3421 ctagggattg gtgatatttt agatgaagca gtgaagtatt tctctgaaga gactgaagaa
     3481 gaggacgacg acacgattaa gtttagtttg attggtcgcc caaatgttgg taaatcctct
     3541 ttgatcaatg cgattttagg agaagaacgt gtaattgttt cggatattga aggaacgacc
     3601 cgagacgcaa tcgacaccca cttcgtttct gaaagtggtc agaaattctt aatgatagat
     3661 acagctggta tgcgtaaacg cggaaaagta tacgaaaata ctgaaaaata cagcgtgatg
     3721 cgtgcaatgc gtgccattga ccgttctgat atcgttttga tggtactgaa tgctgaagaa
     3781 gggattcggg aacaagacaa gcgagtagcg ggttacgccc atgaagctgg tcgtggaatt
     3841 attatcgtcg tgaataaatg ggatcttgta aagaaagaaa ccaatacaat gcgtgatttc
     3901 gaacaagaga ttcgtgatga attccggtat cttgattacg ctccaatcgt ttttgtttct
     3961 gcagtgacta aacaacggtt ggaacgtctg cctgaaatga tcgaacaagt aagtatgaac
     4021 cagaatcttc gtatctcttc ggctgtgttg aatgatatca ttatggatgc tgtagcaatc
     4081 aatccaacac caacagataa aggaaaacgc ctgaagattt tttatgctac acaagtcgct
     4141 gtcaaaccac ctacatttgt cgtttttgtc aatgaagagg aattgatgca tttttcttat
     4201 gaacgtttct tagaaaatca aatcagaaaa gcatttactt ttgaagggac gtccatccga
     4261 attatccctc gtaggagaaa ataggcattt taccatatcg acgttttttt ttcaaaagaa
     4321 aaagacagtt tcgtttgtta cgtttctatg actaaaaaaa gcatgtgaaa attggtaaaa
     4381 accttgctat taagcggttc atatgatatc gtgataacag aatattgaag ttaatcaata
     4441 tttcttttta aacttctgga ccactcagaa agttttaact aaatgatatt tctatcatat
     4501 ctcttgtgga ggaggtgaat ctatccatgg caaacaaagc agaattaatc gaaaaagttg
     4561 cttcagctac tgatttaact aaaaaagatg caactgcagc agtagatgct gtattttcaa
     4621 ctattcaaga tgctttagct aacggcgaaa aagttcaatt aatcggtttt ggtaactttg
     4681 aagttcgcga acgcgcagct cgcaaaggtc gtaacccaca aactggtgaa gaaatcgaaa
     4741 ttccagctag caaagtacct gcattcaaac caggtaaagc attaaaagac gctgttaaat
     4801 aagacagttg aaaaagcgct tgtggctcta gggctacaag tgcttttctt tttatttcct
     4861 agcagaaaag gaataaataa gagacagaga ttccgaatcc gtatgtcata catagaaaac
     4921 acatgtattt tatttaattt ttatttattt gcaagttatg aggtgagctt tgctcttatc
     4981 ttttctagaa tttgcttact aatggtaaaa taaaaataag aagaggaggg atgcgtatgg
     5041 aacttgatta tcaagtagaa gatgcgttga cgaaagtgtt ttacggagaa acgctagtca
     5101 gtcagctaag agaactttcc attgatcagc caatcttatt tttaacgaat cagcgctatt
     5161 atgatttgtt tgcagataag atcaacccgt tatttgcaaa tcaagcaaac aatgactggt
     5221 atatttgtgc caacactcaa tgcaatcatt taacagaatt aaagaacctg cttgatttcg
     5281 tcaaacgcta tcccgaaaat caatccatgt ttatcatcgg atttggaaat gaagggatca
     5341 tggatctggc aggttttttt caaaaacata caacccttga ttcatcacta tggatgatcc
     5401 ctgtatcgat ccgttctcta agccgagcat taacaccgca gcggacgatt ttgcagctac
     5461 caaatcgtgt agtgctgcaa accatcaatc tgccagaacg catcgtttat gatcaaacaa
     5521 tcagcgagcg gcaggtggac ggaaagcaga ttgacttttt gacttttatc tgttgcggaa
     5581 tgatttgtga tcatcgtttt ttgcaaaatc tgtataaaaa ctatccaaat aaaaagcaat
     5641 taacgactcg gccgtttgcc gggatgatcg aagaaatgat ctatttttat caattagagg
     5701 cagaaaacct gtcaaattat ggtaaactgt ttgaacaagc tttttatctg accgaaaatg
     5761 gccatctact ttctgctagt atgaaaaaat ttttaggaat gatgtttcat ttcgtgtgga
     5821 atctccaagc atatcctttt gatttccaac taaaaaactt ttttgtgtgg ctggacttgc
     5881 ttggctatcc aatccactgg ctggaacaga tttcaaagat ggagtatttg gaaaaagttc
     5941 tttttttggc agaaaaaagc aaaaaaatac ttgttttaga aaaaataggt ataatagacg
     6001 gataccaaca tccaaacgaa aaagaattat tgagaacaat gacggtattt caacaaatcg
     6061 tcagtgagat aagagggaat tgaaatgaat acaaatagcg aaaaaatgtt gcaagcttta
     6121 tcagatgaag acttgtcaca agcacaaatc tttttagaaa aagcattaaa agaagaccca
     6181 gcagatatat tagctgaact aggcgaagaa ttgctggcta ttggcttttt gatcgaagca
     6241 aaacagattt ttgaacaact gatcaaccaa tacccagaaa atgacggact gaacatcccg
     6301 ttagcagaaa ttgcaatcga agataatgag atcgacttag cttttgaata cctggaaaaa
     6361 gtggataagc aaagtgatta ctatccgcaa agcttattgg cgatagctga tttgtaccaa
     6421 gttatcggaa tccctgaagt gagtgaagca aaattgaaag aagcagctgg gttgcttcct
     6481 gatgaaccat tgatccagtt tgcgctagct gaattgtatt tttcagttga tcgtttcaaa
     6541 gaggcagcag tggtttatga aacattgttg gcaagcggga taaatgaaat ttcaggaatc
     6601 tcgatgcagg aacgtttagg acaatcctta agtatgcaag gagaattcga agcagctatc
     6661 gtacctttag aagaagcact gaaagaagaa aggacagatg atcgactgtt ccagctagct
     6721 tttacgaatc ttcaattgaa agaaaatcag caggcaatca actatctgga agaacttcga
     6781 gaagttaatc cgcattatca atcgttatat ctatatctag gacaagctct tcaagaagaa
     6841 gaaatgattg aagaagcaca aacagtgtta gaagaaggaa tcaaagaaaa tccttaccaa
     6901 gtcgaacttt atcacctggc ctcggaaaat gctttccgtc tgcatgatag acagcgagca
     6961 gaagaacttt tactgaaagc tttagaagta ggagacaaac aagatgaaac attacttact
     7021 ttaagtaatc tttacttgga tgatgaaaga tatgaagatg tgatacaagt gatcaatcag
     7081 atggaagaaa ctgctaatcc ttatgcagaa tggaacctag cccatgctta taatgaatta
     7141 gaagacttta ccgttgcagc tgtacactat gaacaggctt atcatgaact atcacatgaa
     7201 ccagatttct tgaaagagta tgcctttttc ttaagagaag aagggcagct gaaaagagcg
     7261 caagaattat tgacttatta tttaacactt gaaccaggag atatggaggc actgtcatta
     7321 ttagatgacc taacagagag gtgagaaaaa tgctaattga tgtacgggaa aagaagaatt
     7381 ttttaacgtg gatggtcaat catgtatctt ttagccgaag agaagttttt tggatcttga
     7441 attatcttgc caatcatgaa gcgattttaa gcaatgtcca ctttgttgaa ggggcgaatt
     7501 ttgcggatcg aggactgcag atcagtgatc tatctgttga aggggaaccc atgaaattgt
     7561 ttttgcaggg aaaagaattt acggacacag accagatttt tcatgaaatc cgtttgaatt
     7621 ggaaaaagcc tttatatgtg gaatgtatat tcgaaaatgc ttggcagaca agagaatatc
     7681 tatctatctt agaagacaat ccattttctt cttgggattc ttcagtcagt gaagaagata
     7741 ctgaattaat tgaacgatat tttcaagaaa aagaagaaga ggcaaaacta aaaatgcttt
     7801 atgctcaaat cgatcaagca ctagaagatg gagataggga tgcttttttg aaattatctg
     7861 atgaagttaa ccgaaagatc cttttgcaga cacaaaagaa aagtaaccgg gtaagtgaac
     7921 ataacaatta gttaaaaata gtatagtact aaaaaagaca aaggtcagct ggatgtggaa
     7981 tctttcctca tagcctatct ttgtcttttt tcttatagtt ttttcagtaa tcttgtctgt
     8041 ggcttcaaat aagtaaagcc ttgcccttcc acttcatgca cattgatgat tgaaaggaaa
     8101 gctttttcgt caatctcgtg gacgatcctc ttagcttcag ttaattcact agagctaacg
     8161 accatataga tgattttttt tgaattacca gagaatgcgc cttctccaga aaggaaagtc
     8221 acacctcttt gcagaccggt catcagtagt ggtgcaatct cttcgctttt gttggagacg
     8281 acaagtagac cttttgcaga atatccgcca tctaagatcg aatcaatcac acgactgaaa
     8341 acatacgaaa caatcaacgt atacatcata cgtttcaagt cgatataggt taatgaagca
     8401 agtaagacga tgatatcaaa tgccaatagg gaacgcccca tagtgatgcc gtaattttgt
     8461 tctaaaatcc gagcgataat atcactgccg cctgttgttc cacccatgcg gtagacgatc
     8521 ccgcttccta cacctgcaac taaacccgca agtagagaaa cgatcagtag atcatgctgt
     8581 aaattgattt ctagtggaac cctttgccag atccataaaa agaatgacaa agaaagtgta
     8641 cctagaacag tataggccaa ggaacgttta ccgaggatct ttcctcctag taaaatcaat
     8701 gggatgttga ttaataaagt cgtataagct ggatcaatac caaataaagc tcttaagatc
     8761 aaggtgatcc ctgagactcc cccttctgct aaatgattag cgatgttcaa gtaaacaaga
     8821 ccaaatgcat aaatacttgt tcctataatg ataaaaagaa cttcttttac tgtttttcgt
     8881 ttgaccatat gaattccagc tttcaattat gtattttcac agaatttcca tttagaaatt
     8941 ccttcctaaa tgtagcatgg aaattcgatt ttctcaattg ctcttatcta ggatttgcgt
     9001 taaagttttg gtaagatagt tttggaggga aaaagatgga taaaaagcgt tctttataca
     9061 gtatgcagca ggaagtggat gaatatatcc agcaattcaa ggtggggtat ttctctccat
     9121 tagctcagat ggctcgatta actgaagaag taggtgaatt ggcaagagaa gtcaaccaca
     9181 cttatggaga aaaaagcaaa aaggcttctg aaccagttaa ctcggtagca gaagaacttg
     9241 gtgatgtttt attcgttacg atgatcatgg ctaattcatt gaatatcgac ttaactgaag
     9301 tttttgaaaa aaacatggaa aaattcaatc aaagagacca taatcgcttt gagcgtaagg
     9361 acgagtaaac aagatgaagt ggtcctggcc ttgaagactc tgaaacggac ttgtcaaaca
     9421 gtcaccatgc aactgagtct ttatctcagg acaagaaaca cgcaggaaag gttggaaaaa
     9481 tgaaattaaa acaattacct ctggaatacc aaaaggcaat tccagttctg aaaaaattag
     9541 agaatgcagg atatgaagct tattttgtag gtggaagtgt tcgtgatatc ttgctgaacc
     9601 agccaatcca tgatgtggat atcgcaacaa gcgcatttcc agaagaaatc aaacagcttt
     9661 ttccaaaaac gatagatatt gggatcgaac atggaactgt gttggtatta gaaaatgatg
     9721 aacaatatga gatcaccaca tttcggacgg aatcaactta tcaggactac cgtagaccag
     9781 atcatgtcga attcgttcgt tcgttggcag aagatttgaa aagaagagat tttacgatca
     9841 atgcgtttgc tttgaaagaa gatggagaaa tcatcgattt gttcgatggt ctgcaagatc
     9901 tagaagaaca gatcttgcga gctgtaggca atcctcatga gcggttccat gaagatgcct
     9961 tacgaatgat gcgcgggttg cgttttgtca gtcaactagg gttcaaatta gaggaagaaa
    10021 ccttctacgc catcaaagaa aaccacgagc tattaggtaa aatatctgta gaacgtataa
    10081 atgtggaatt catcaagatg ctcttgggaa atgaacgggc cgcgggaatc catgcattta
    10141 ttgaaacaga gtgctacatt tgctgtcccg gtttgaaaga agaaggcgaa gcacttttac
    10201 gttttgctga attaccatcc gtcccattga aagaagagag ccaagcatgg gcattattga
    10261 tcagccaact ggatctaaaa gaaaatgaga ttcgttcctt tttgaaatcg tggaagtgtt
    10321 ctaaccaaat gattaaagac gttcaagcac ttgtccaagg actacgacaa cgagaaactg
    10381 gagctttgga accattcgaa ttgtacttct taggcaaaaa acatgcactg caagtagaag
    10441 aattgatgat ctattttgac gaaacacctc agatggatgc cgtaggagaa tcctatgatc
    10501 gtttacctat caagtcatta agcgaattag cagtagatgg taaaatcttg ctagaagaaa
    10561 ctggaaaaaa acctggtaag tgggttagtg aagctttgca attagctgaa aaagccgtcg
    10621 tagaacaaag aataaaaaat gataaaaaac aggttttgga ttttttatcg aaagaaaaat
    10681 tgatttaaaa aacactagac tgtatctgct cagctttgtt ggctacttaa agtgggcaga
    10741 tacgtcatta gaagaaaaaa cataagatgt gaccacttac ataaatttgt gctaaactga
    10801 ggtagtacaa gaataaatta catcataaag gagtcttttt aatgagtaaa gaaatgacag
    10861 cgctaaaatt ttattttaga aatggagaaa cctggacgat caaccgccgt catatcggcg
    10921 atctttggat caaacaaatc acaacaagtt ttggacgaat caatggcagt gaatttgttg
    10981 aaatccatcc gtgtgctgga tttaaaatcg aaatcttcca tgaaggggac gcagttgcga
    11041 cacatgatat caacctaggc ggactggaaa tgggcatgtt taatcgcgca ttgaaatacg
    11101 aagatatcga gcgaatggaa atcctttacc gaaatggcac acctgatctt gtgtacttcc
    11161 catatctaga taaaggaaca gaaggattag acaatcaata tcaatcaaca aaaatcagcg
    11221 aaaaaacagg taacttgtat atcgtgatca atccagatca acgtgtagaa gatgtatacg
    11281 gggaattttt cgaataaatc gatttttacg caaaaatgag gctgtgaaag agatgtgtct
    11341 tattactact gataggctgt ctcttccaca ccgtttattc agaataaggg actgtgacaa
    11401 gatttttgtc gcagccccat ttttttgttg tgataaaaga acaagaaagg caggaatggg
    11461 aaagtaaaca tccttttgtc agaaactttt atctttctta agttttactt gatatctgat
    11521 actatttgga atagaatgag gcgttgaaaa atttatggaa atttctaact agcaataaac
    11581 gatgaaacaa tgaaatattt ttcatccaaa aagaactttg ctataatgat ggaagtttgt
    11641 gtataggaga tctggaaaat gagagagagt tttgaacaac aaaaaaaact gttgcatgat
    11701 cgctatggcg ccctttcgat ggatgaccgc cgtcaaatac tttgtaagtt gcgtaaaaga
    11761 aatatattga tgtatcgtca gttggaacgt ctgaaacatg atctgcttcg tttggagtca
    11821 aaacgtgtac agtgtgaatt agaggggaac caaacacagg ttgaagtagt cgaaaccaaa
    11881 attttaaaga aaaaagaaca atttttaaaa atgcttacac aaaataaaaa ataaagggga
    11941 tggacgatgg aatttgctga gttagttatt gtatttgcag taacgatcac cttatcgaac
    12001 atagctagcc gcattttgcc aatgattcca gctccactga tccaaatttt ccttggagtg
    12061 gtattaggtc tgacagagtg gggacaatca atcgattttg aacctgaatt gttcttagtt
    12121 atgattattg caccattgtt atttagagaa ggagaaaaag cagatatttc ttcgattctc
    12181 aaaaatttcg ggacaatttt gttcttggca tttggaggag tcattttgac ccttgtcggg
    12241 gtaggtgcga cgttatcatt cctgttgcca agtgtcccac ttgctgcctg ctttgctttt
    12301 ggagcagcgt taggtccgac agatgcggta gcagtcagtt ctctttctgg acgagtgaat
    12361 atccctaaaa aggcgatgca tattttggaa ggagaaggtc ttttgaatga tgcatctggt
    12421 gttactgcct tccagtttgc tcttggtgcg ctgatcaccg gatctttttc agcagtaaac
    12481 gcggggatga gtctagtagt ttctagtatt gggggagctt tgatcggctt tttgcttgtc
    12541 tggttcaaac aaaaaataat tcatttgatc gaaaaagcat cagcacaaga tgtcacaggg
    12601 tatctgctga tcgaactttt gcttcctttt ctagcctatg tattggcaga ggcatttgat
    12661 gtatctggaa tcattgctgc agtggcagca ggtattctcc aagcttctgg ttttaggaaa
    12721 atctctgtgt ttgatgcaga attgtcgagc ttgtcccaca gtacatggac aactattgct
    12781 tttacactga atgcattagt atttatattt ttaggaatcg aattgacgca agtgttctcg
    12841 ccagtatggg gagacggttt atatccaaac ggtcttttat tagcgattat cgtattaatc
    12901 tctgtcatgc tttttgtgat cagattcatt tctatcagtc ttttttatgt attcaaagat
    12961 ggcagtaaaa agttcaaaaa acagttgaac gagatcttga tcttaacatt tggcggtgta
    13021 aagggaacag tcagtttggc tacgattttt attttgccat tttcaatcaa taatatgatg
    13081 ttttaccagc ggtctttgct tttgtttctg acggcgggtg tcatcttagt gactttggtc
    13141 atagggatca ttgtcttgcc tatgctgacg gaaacagagg aagcgaaaag cacggatttg
    13201 aatgctttga tgattttaga agaggtcgtt gaagttttgc gaaaagaaat aaaggaaatt
    13261 gatcgtaata caaaagaatt tttagccact gaagcagtga tagaaaatta tcaagaacgg
    13321 atacgtgatc tgtacttgga agatttaacc gatgatgaaa agcaagaagt acaagaaata
    13381 caagcactga ttttgtcgat cgaacgggat ggattggatg aaagttatcg ttcgggcaag
    13441 ctttcttcta atggttatcg tttttactcc cgttttcttt ctcgattcga acattctatc
    13501 acaagccaga ttttgtcttt tatcggcttt tggttcattg ttgtccgtag gttaatgcga
    13561 atcgttctcc atccgaaaat gttttggcaa agaagatatg ctgatcgtca gacatttatt
    13621 agtgatgaag atatccaaga aatcagagag acatatcaaa aaaatactca gttgattatt
    13681 gaaagtttag ataacttgac tgatatttat gatgaaacga tggttcaatt ttttatccag
    13741 caacgcaaac ttgaaggaat aaaaatgaac tcggggaatt tgatttcttc gtggatgatc
    13801 cagcaagata gtctgtttac gaaaaaaatg ctaagaggtt attatcttga aagaaaaatc
    13861 attgatgaat acgaagttgc tgaaaaaatc acgacgtttt ctgcgaacag ctatcgcagg
    13921 aatatcaatt tgttagaatc ttatacgatg aacaaaccat ctgataactt ttctttccga
    13981 tttgcgtttc gagcaaagtc aaaaaaaacg agtccaataa aataaaaagg taaaaatttt
    14041 caagttttat aaaaaacaaa acatatcaga acaggatcga atcggtcctg ttcttttttt
    14101 gagaacacaa aaagaacaaa taccaaaaaa attttcctga agttcactat ttttatgata
    14161 caatatccaa gataaggagg gaacatgttg aaagaattaa aagtaactga cctcaaaaaa
    14221 acttatggtg aaaaagattt gtttgaccag atttcttttt tggtccacga aaaagaccgt
    14281 atcgggttga ttgggacgaa cgggacgggt aaaacaagtc tcttaaatat tattgccggt
    14341 atcgatagca gcgacggtga tcggcaaaca gtgttttatc caactgatta ccgtattggt
    14401 tatctttctc aagcagctga attttccgat gaattaactg ttctccaagc agtctttcaa
    14461 ggaaacagcc ccttgatcca aactgttcga gcatatgagg aagcattgat cgaactaggg
    14521 gaaaacggag aagaccctga tgtgcaaaaa cgttatgcta aagcagaaga acaaatgaat
    14581 aaagaagatg cttggactac agataccaac gcaaagatta ttttgcaaaa attaggaatc
    14641 agtgaattag ataaaaaaat cagtacttta tcaggtggtc agaaaaaacg agttagtctg
    14701 gcgcaagtgt tgattgatga acccgattta ctattactag acgaaccaac aaaccattta
    14761 gattatgaag cgatcgagtg gttggaaaac tatttgaaac aataccgtgg cgcactattg
    14821 atggttaccc acgatcgtta ttttctagat agagtagcaa accgaatctt cgaactttca
    14881 tttggaaaat tatacgagta taaaggaaat tatgaagctt acgtcttaga aaaagcagaa
    14941 cgtgatcgtg tagaagtaga acaagaagaa aagcgcaagc ggctttataa acaggaatta
    15001 gcttggatga gggctggcgt acaggcacgt ggcaccaagc agcaagcacg gatcaaccgt
    15061 ttcgaagatc taaaggagaa cttgtaccaa gtaaaccaag aagatgattt agagctgaac
    15121 ctagcgacac aacgtttagg gaaaaaagta ctggaaatca aagatggcag ttaccgtata
    15181 aatgaccaaa cactgctaga acatttagat ttattgattc aatctcggga aagactaggt
    15241 attactggta aaaatggtgc gggaaaatcg acattgctta atattctagc tggcagaatt
    15301 cccctggata gcggtacaat gtctatcggt gaaacggttc atcttgcgta ttatacacaa
    15361 gaaaatgaag aaatggcacc agataaacgg atgattgctt acttacaaga agctgctgaa
    15421 gaagcaaaaa cagcagatgg cagtcaaatc ggagtagcag aattacttga acgattttta
    15481 tttccaagat ttatgcatgg gacactgata ggcaaacttt caggcggaga aaaacgccgg
    15541 ttatttttgt tgaaactgct gattcagcaa cctaatgttt tattattgga tgaaccgacc
    15601 aatgatttgg acatagcaac attgacgatt ttggaagatt attttagaag ttttccaggt
    15661 gctgtgatca ctgtttccca tgaccgttat ttcttggaca aggtagcaga taagttatta
    15721 gtattccaag gaaacggaaa acaagaactt tattacggaa acatgtccag ttatttactg
    15781 aagcaaaaag agacccagca gcctgctgaa aaagcgaaac caaaaataca gagcaaggaa
    15841 cctgctgaaa agaagaaatt atcttacatg gaacaaaaag aatgggaaac gattgaagat
    15901 gagattgcag aacttgaaga aaaaatctca ttgctccaag aagagatgaa tcatcaagga
    15961 gataatttca ctcgattaca agaattgcaa aacgacgtat ctgaaacaga agcacaactt
    16021 gaagaaaaaa tggcacggtg ggaatacttg agtgaatggg tagaagatta ggaggaaaaa
    16081 ggatggaaca agcgtatctt gacttaggaa gaaaattgtt ggaagaaggg catcgtaaag
    16141 aagacagaac aggcacaggc acattaagct tattcggata ccaaatgcgg tttgatctgc
    16201 aaaaagggtt tcctttgtta acaacgaaac gagtaccttt tggattgatc aaaagcgaat
    16261 tgctttggtt tttaaaagga gatacgaata tccgctatct attacaaaat aaaaaccata
    16321 tttgggatga atgggcattt gaacgctaca tcaaaagtcc agactataat ggaccagata
    16381 tgactgattt tggccataga tgtttgacag atcctgaatt caatgaagaa tataaaaagc
    16441 agagcaagct tttttgcgag aagatcctaa ccgatgattc ctttgctgaa acatacggcg
    16501 atcttggaca catctacggc tatcaatggc gtcactggga aacaaaagac ggtggcttta
    16561 tcgatcaaat caaacaagtg atcgaagcaa tcaaaaagac tcctgattct agaagattga
    16621 tcgtttctgc atggaatccg gaagatgttc ctagcatggc tttgcctcct tgtcatacga
    16681 tgtttcaatt ttatgttcaa gagggacggc taagctgcca attgtatcaa cgcagtgggg
    16741 atgtgtttct aggcgttccg ttcaacattg caagttatgc gttgctgact cacctaattg
    16801 cacatgaaac aggtttagcg gttggcgaat ttgttcacac attaggagat gctcatcttt
    16861 atatcaatca tatcgaacaa atgaaagaac aattgagccg agaaatacgc gcatttccga
    16921 ctttagtttt gaatcaggaa aagcagtcag tatttgattt tgagatggaa gatatccaat
    16981 tagaaggata tgacccacat ccggcgatca aagctcctat cgctgtttag aatcagaaag
    17041 gatgagaaaa tgttagctgc aatttgggca caggatgaaa acggattgat tggaaaagaa
    17101 gaccgtcttc cttggcgttt gccaaatgat ctgaaattct tcaaacaaat gacagaagca
    17161 aatacactag tgatgggacg aaaaacattc gaagggatgg gtagccgtcc gttgccgaat
    17221 cgtcagacga ttgttttgac gagagattct tcctataaag cagaaggcgt gcatgtcatg
    17281 catagtgtgg aagacgtgct tgctctggaa aaagaaacag acggtatctt tttcatcgct
    17341 ggagggtcag ctgtttatca agaatttctg ccattttgta cgatattata ccgtacagtc
    17401 atccatcatg tattcgatgg agatgcttat tttccacctg ttgactggtc tgattggtca
    17461 ctgatcaata ctagtcaagg agaaatggat gaaaaaaatc gttatccgca tcaatttgaa
    17521 acgtatcagc gtaatgagaa tcctgtaaaa agcgggacag gcgaattgac gatataaaaa
    17581 agctatggat agactagctg ttatttagag ctgtcccatc catagcttta ttttttttat
    17641 taagtaaaga ataaaacaga gaagtacata aaacctgcac ctaacataac aaacaaatgc
    17701 caaacaacat gcatatagcg gatagaattc aagctataaa agaatgcgcc tatcgtgtaa
    17761 gaaagcccgc ctgctaccat caaaataaca ccagtcattc caagagagtg ataaagagga
    17821 acagcagcca cgacacagag ccaccccata aaaatataga tgatcgtaga tacttttgat
    17881 actctctccc gtttatgcaa cgtcaacgct ttatatataa tgccaagaat cgcaaacaac
    17941 cagattaata caaataaagt ccaacctagc catcctcgga tactcagcaa acaaaaagga
    18001 gtataacttc ctgcaatcaa aagaaaaatc gaactatgat cgaatacttg gaacaccttt
    18061 tttgcttttg taaaaatcaa tgaatgaaac aaagtagacg ttaaaaataa aagaatcatc
    18121 atcgatccat agatagcata agagacaata tggagggagg aatgcaagtg ggcacctttt
    18181 actaaaagaa ccactagtcc cgcgatactc aatccagtcc caattccatg ggtgactgca
    18241 ttaaacactt catttaatat ttcatacgtt cgattaaatt tcgttttttc catgaaaacg
    18301 gctcctttac taggtgtaat taatgaaaaa tcattatttg tctgttatac taaaaataga
    18361 aattgtctta catgtagttt aacatgaata aggcatcttg ttaaagaaag agagagagaa
    18421 aataatgaca aatgtaaaaa tcgtaacaga ttcttcatgt acgatggaaa aaaatgtacg
    18481 cgatgcttta ggcattcgtg tcatgccgct atccatcatg atcgacggaa cagtatatcc
    18541 cgatgatgac caactagacg gcgaaagatt tatggaaatg atggcgaaag caaaaaatct
    18601 gcccaaaaca agccaaccac caattggaga attcatctcg atgtatgatg agctaggaga
    18661 agacggcagt gaagtgattt cgatccatat gacaaaaggc ttgagtggaa cagtagaagc
    18721 agcaagacaa gccagtaatc taacaaaaac gaaagtaacg gttatcgaca gtgataccac
    18781 tgaccaagga ttatcttttc aggtaattcg agcagctgaa ttagcaaaag aaggtaaaac
    18841 agtagagaaa atccttgaag aagtcaaaaa agtacaagac aatacaaaat tatacatcgg
    18901 aatatccaca ctagataact tggtaaaagg tggaagaatc agccgaacaa ctggtttgct
    18961 gtctagtgtt ttgaacatac gcgtggtgat gaatttcgcc cattctgaat taatccctgt
    19021 aactaagggg cgaggaaaga aaacgttcga taaatggttt aataatttga aaaatgaact
    19081 gaaagaattg ccaaatgtgc gacaaatcgg tatttcacat gcggatgcaa aagaattggc
    19141 gcaagaattc aaagagggtt tgcaagaatt attcccggac atgcatatcc ctgtactgca
    19201 tacgaatccg gttatcgcta cgcatactgg taaagacgcg tttgcaatca tgtattatgt
    19261 agattagtcg aaaagaggat aagccctcag cctgaaaggc ttgcagctta ttctcttttt
    19321 ctttataaaa ttaggaaaat aattgttttc ttttttcata aagactacct ttttgataaa
    19381 atttttgata acattagaac caatcgaata caagaggtga aaaaatgaaa ttaacgaaac
    19441 gacatttgat cctcgttttg ttcccattat taattgcgat catcatctat gcaggtttag
    19501 ctgcttctat accgaaagct ggcccactaa tgaaaccagt aactactgtg agagaagatc
    19561 caaataaaaa agaaaaactt cactacatag ctatcggaga ttcgctgact gaaggagtag
    19621 gagatcaaac caagcaagga ggattcgttc cccttgtcgc aaatgatcta aaagaccgtt
    19681 ataatttgac cgcggttgaa acagaaaact atggtgtaaa tggtgaacga agtgaccaga
    19741 tcttaaagcg agtaaaaaca aaagaagaaa ttcgaaacaa tatcaaaaca gctgatttta
    19801 tcacactgac tgttggcggt aatgatttga tgaaagttat ccaaaataat ttattcgggt
    19861 taacggtcaa ttcctttaaa aagccactga aaaactatca ggaaaatgtc acagaactat
    19921 tggaagaatt tcgatccctc aataaaaccg cacctattta cattttaggc atctacaatc
    19981 cattttattt gaactttcct gagatcactg acatgcagac aattgttaac aattggaaca
    20041 aaggaacaga aacggtgaca aaaaaaatgc cgaactgcta ctttatccca atcaatgatt
    20101 tgctttatca aggattgaat caggaagtag gtgtcacaga agaaggaaca gatcaaacaa
    20161 ctagcacgac agaaaatcta aatggcatca aaaataatgt gctctatgaa gaagatcatt
    20221 tccatccaaa taatttaggt tatcaattga tggcaaatgc agtgagagat cagctgatcg
    20281 agacacaaaa ccaatggtta gagaaggagg atgatcaata aacatgaaaa caagatctga
    20341 cgtatcttca aacaaaacaa agaaaacaaa ttactggaaa ttcgccttcc ttatccttct
    20401 tggggtagta cttggttcga ccgtattttt aggcagccgt atttttgcta atcgggaacc
    20461 agaactccca gaaataccag ctcttacgga aagacaagga gatcctgtct taacgatcaa
    20521 ttcaaataaa gaaaaagtca atcaaatcat cagctttttc cttagtgagt atcaaaaaga
    20581 tagtgatatt gaatataaat tttatttgga aaatgaagca ctactcaatg gtactttcga
    20641 agtactaggt tttcctatcg atttctattt atattttgat ccgtatgtga tggaaaacgg
    20701 aaatgtccaa ctaaaagcta gaagtctttc tatcggaaca ttgagtttgc caatcaaaga
    20761 tgtgatgaat atgatcaaga gaaactataa attaccagat tggatcgaag tcaatacaga
    20821 tgatctgact atcatgctta ggttggatca attccgaatg caaaacggga tgtatattaa
    20881 agcagataaa atcgacttag taaatgatga cattcggttt agtctatatt tgccaaaaga
    20941 tacctctgat gcttcaaaca actcaaatac caacaaaact agcaccagca gtactgaaag
    21001 caagacaaaa gaaaataaaa aatcatcgga tgagtagtat tagaggggga atcataatgg
    21061 aacgagcaat atttgcagga ggctgcttct ggtgtatgat ccagcctttc gacacgcttc
    21121 ctggtatcca tacagtgatg tcaggatata ctggagggca tgtaccagat ccgacttatg
    21181 aacaagtaaa aagccaaacg actggacata cagaagcagt ggagattatc tatgatccaa
    21241 cattgatttc atatgaacaa ttagtagaac tttactggca gcaaactgat ccaactgacg
    21301 ctttcggaca attcgaggat cgcggtgaca attatcgccc ggtgatcttt tatttgacag
    21361 aagaacagcg gatcattgct gaaaaaagta agaaacagtt agctgaaagt ggacgtttcg
    21421 aacagccaat cgttacaaaa atcgaaccag cccaaccatt ttatctagca gaagaagacc
    21481 atcaagagta ttataaaaag catcctgaaa attttaaaag aaaccatgca cgccgggctg
    21541 cctttatcga agaaaattgg aggaacgaag ggtgaatcga agcttttttc attatttaat
    21601 gacactgcga ggcggtggca cacatgatgc tttatctgtt ttcgctacag aagccggtaa
    21661 agacatccaa tttcctaagc acagctctag ctacgaagaa atttctaatt atctggaact
    21721 gaatgtcgac tatttgccgt ccatggatat ctttgacgaa gcatgggaaa aatatttaga
    21781 aaataatcat gacatataag agatagtttt gtactttatg atcaaaaaag ttttttgtcc
    21841 aatagcctaa ttgaaagaat tatttttatt tcagctatct agtcgcattt ttttgatatt
    21901 tttcttataa taaagaaaga atgaaaaaaa gagggatgga ttgatagaga ttgacaaaaa
    21961 aacaacgcta tattaatcgc ttctggcttg tattcaaata cttacttgtt tctgtattcc
    22021 ttgcgttcat gttgcgggga tttttattca tcccggtacc agtggaaggc aattcaatgg
    22081 aaaacgtgtt gaagcaaggt gatatggttg tgatggaaaa attttcggag atcagacgat
    22141 ttgatattgt tgtttttcaa ttagcagatg gcacgattta tatcaaacga gtaatcggtc
    22201 ttccaggaga aaatgtcagc taccaaaatg atcaattgaa gatcaatggg aaagtagtta
    22261 aggaacctta tctaactaaa aacctgaaat cagatcatgc aaatgcatcc tatacgacgg
    22321 actttacact acaagagcta acgggacaat ccaagcttcc ggaagataat tattttgtac
    22381 ttggagataa tcgaagagtt tcaaaagata gtcgttcgtt tggcacgatc aataaaactg
    22441 atattctagg taaagcaaga tttgtctatt atccacttga tgaaataaaa tggatccagt
    22501 gagaaacaat aaagcgtgca gtctgttgag actgcacgct ttattgttta atccaaaatc
    22561 agtttactac cttagtacgt aaaatcaaac agctcgttta aacacttttc tagcctctaa
    22621 agttttgata aatgaataaa caaccattat ttgaataggg atttggatca tattcctgat
    22681 gaatctacct agaaaaatta ctttgaaatc tgcaccaaac aaaacagaca tcagccaaga
    22741 attgataaaa aggctgactg ggatcataat caataattgg gcagcgatga cggaaggcag
    22801 gtttatcttt ttatgatata aaaataagcc attaataaat ccaccaagga aagccgtgat
    22861 aagaaaagca gcttgaaacg ttcctgaacc aaatatcgtg aaatttaaag tgttcgacaa
    22921 catttcaagc aaaccagccc agatcggtcc atatagataa cccataagcg ccatacttaa
    22981 aaatccgaat gtgatttgaa aataagcagg accaatcgct atcttgtata agatgacatc
    23041 aagggctaat aaaagagcag aggtcgacaa caatcttgca ttcatttcgg gcaatctaaa
    23101 aacgtctttt gcaatcaaag tgttcaaaat attctctcct ttgtcagtta ataataaaag
    23161 gataccataa aatcaccaat gtgtcatgac acattggtga tttacataca ctggcatcta
    23221 acaattaaaa aaagggtgag aagactgctt ttataaactg taaaaaagag attgtacgaa
    23281 taaaaaaaat agaggcggag aatacttatc gtatcatccg cctttgtttt tttgttataa
    23341 aaaacgtcag attagttatc tgcttacatc ttcatccata cgggttctaa tgtatttccg
    23401 tctagatctt ggacttctaa tgaaaacatt tcttcttcag gcattcccat atctacttcg
    23461 tggaaagtac caccattttc ttttgcaatt tgagcaaatt tttttacttc atccgcattt
    23521 tcaacactaa acgaaatcaa agcaccactt gtttttttat tatcagctat ttctttatct
    23581 tttataaatt ttttataaaa atcatgagag agaagcatta tccaaaaagt gtcatcccat
    23641 accattgaac cagcttcttc agtagaaaat tcttcatttt ttttaaaacc cagcttttca
    23701 taaaaagcaa cggaacgatt gacatcagca acaggaaaat taacaaaaac catactattc
    23761 atttgtttcg tctcctttct gttttgttcg tctatcatct cttatttagg aaacgctgtc
    23821 aaaaatttag attcatatat agaaaccaaa ataaaaattt gcatcatcaa aaaagtgaag
    23881 atgtgataga atgaggactg gttcactttc acttaactaa tagatggaat ttctttataa
    23941 taaaaaagtg aatttatatt gaaaaagaaa aaaacaagta aagccgggga atattccggt
    24001 atgaaaggaa tggagagaac aatgaccatc caatggtttc cgggacatat ggcaaaagcc
    24061 cgacgagaag tttctgaaaa aatgaaatat gtggacatcg tatttgaatt ggtagacgca
    24121 cgattacctt tatcttctcg taatccaatg ctagatcaga tcattcagca aaaacctcgc
    24181 cttgttttat tgaataaagc ggatctggca gatccacaac agacaaagct gtggcaagaa
    24241 tattttcgta aacaaggcca tcttgcatta gctatcacgg cacaagaaag taaaggaatt
    24301 aaaggtctag tacctgcggc taaagaagca ttgaaagaaa aacgggcaag agatgctgct
    24361 cgggggatca agcctcgcgc tattcgggca atggtcttag gtattccaaa tgttgggaaa
    24421 tctacgttga tgaatcgact agtaggcaag aaaattgcac agacaggaaa taagcctggc
    24481 gtaacaaaag gtcagcagtg gcttcgttta ggtaatgagt tggaattgtt agacacacca
    24541 gggattttat ggcccaaatt tgaagatgaa gaaatcggca aaaaactagc gctgacaggc
    24601 gcgatcaaag accagcttgt ccatttagat gatttagcga tttacggatt agactttttt
    24661 gcccgatatt attcaggaag aatcagcgat cgttatggtt tatcaatcga gcaagaagaa
    24721 aaattggcac cggaattact gatggatatt acagcaaaac gtgggtataa agaagactat
    24781 gatcgtggca gcgaattagt cgtgcatgat attcgtcaag gaaaacttgg acgatacaca
    24841 ctggatcgtt gcgaagaaat ggagcaaaaa agtgatgact gaatccatcc aaagtatcaa
    24901 agaacgattg aaaagcatac agagaataga tgatcctaca ctagaatcat taagaaatga
    24961 tccacgcaaa ggcgtgcagc aagcactggc ttcttttgaa cgacgattga aaaaggaagc
    25021 attaattaag gaaaaatatg aagaaatgtt ttgctttgaa aagcaagggt acgcacaagg
    25081 tcaccaatgg attgccggca ttgatgaagt aggaagagga ccattagctg gacctgtcgt
    25141 ggcagctgca gttatcttgc cgaaaaatga gatcatatta gggctgaatg actccaaaca
    25201 attatcagag aaaaagcgag aacttcttta tcaacaagtt caagaaaaag ccctggcaat
    25261 cgggatcggg atcgttgatc agcagacaat cgatgatgtg aatatttatg aagcaagtaa
    25321 attagcgatg atccaagcag tagaaaaatt gtccattgta ccaagctatt tgttgatcga
    25381 tgcgatgacc ttggatttgc ctattcagca ggaaaaaata atcaaagggg atgcacgatc
    25441 tgtgtcgatt gctgctgcaa gtatcattgc taaagtatat cgagaccaat tgatgaaaga
    25501 atatgacgtg ctttatcctg gatatggttt ctcgcataat gcaggttatg gaacaaagga
    25561 acacttagaa ggacttgaac gacaaggaat tacgccaatc catcgtaaaa gttttgcacc
    25621 tgtaaaaaaa tatttacaat aaaaaacagc cgcttttacg tatcttatta gaaagagaga
    25681 cgaaaggcgg ttttatgatg tatcaaatag aagaaaattt attgaaactt gcttatacaa
    25741 aaggaatcag tagtcaagga aaatgggcac ttgctaactg gatgatgcag tatccttata
    25801 aagaaatcgg atttgaagac atcattaaga taggtaaaat aaccaaacac cgggagctgt
    25861 ttcgcaaaag ttggcaggaa attcacgcta actggggcaa aatccaatcg aaacagtcgt
    25921 ttatcacttg ctttgatccc aaatatcccc ctcagctgct tcacttgacc tatccgccaa
    25981 ttgtactgtt ttataatggt gatctatctt tactttcttg taacatgtta agcgttgttg
    26041 gtggaagaca cacttctgct ttagccaaaa aaacagttta ttatttacta aaaccagtga
    26101 tagaagctgg atatgtgatc gtcagtggct gcgcgaaagg gatagataca tacggtcacc
    26161 aagcagcaat ctctcatggt ggacggacaa tcgcagtcat tggaacaggg atagagcaag
    26221 tctatcctgc tgaaaatcgt gacttgcaag aggtgattgc aaatgatcat cttcttttga
    26281 gtgaatatcc tcctcaatcc ggcccgaaaa agcattgttt tccaatgaga aatcggatta
    26341 tcgcagcttt gtctaaagga acatgtgtct ttgaagccaa gaaaaaaagc ggctctctga
    26401 taacagcaca gcaagcattg gatcttggaa gaacagtttt ttctgttcca ggaaatattt
    26461 taacagaaca ttctaccgga tgtcatcatt tgatccaaga cggtgcagtt tgcacggttt
    26521 cgggtgaaga tatcttggca gaatttaaag aataacacca tacttttcat ttgcttttct
    26581 tgacaaacgt tttctgaatg gatatgatta gctacgattt gtatgaatcc tcttttaata
    26641 gtaggaacac gacaatggaa aggagtccat tcagtaatgg cgtataaata tttagtgatt
    26701 gtagaatcac ctgcaaaagc caagacaatt gagaaatatc ttggacgaaa ctataaagta
    26761 atggccagtg ttggccatat aagagattta ccgaaaagta aaatgggtat cgattttgaa
    26821 aacaattatg aaccccatta tatttctata cgcggaaaag gcgatgtcat caaaagcctg
    26881 aaagccgcag caaaaaaagc tcaaaaagtt tacttggcaa gtgacccgga tagagaagga
    26941 gaagcgattg cttggcattt agcgtaccta cttgggttgg atctgaaaga aaaaaatcgg
    27001 gtggtcttca atgaaatcac aaaagacgca gtcaaagcag cttttaagga accaagaacg
    27061 atcgatgtag atttagtaga tgcacagcaa gctcgtcgta ccttagacag aatcgttggt
    27121 tattcgatca gtcctattct ctggcgtaag gtcaagaaag ggttaagtgc aggacgtgtc
    27181 caatctgtcg ctttaaaaat tattattgac cgtgaaaaag agatccgaga atttgttcca
    27241 gaagaatatt ggagcatcga cggtaatttt aaaaaagctc gcaagaaatt caaagcaaat
    27301 ttctggggaa tcgacggtaa gaaaaagaaa ttaccagatg cacaaagtgt aaaagaagtc
    27361 actgctagaa ttgatggaac agacttcgaa gttaaaaaag tagaaaagaa agagcgcaaa
    27421 agaaaccctg cgaatccttt cactacaagt agcctgcaac aagaagctgc ccgtaagtta
    27481 aactttcgaa caagaaaaac catgatggta gcacagcaat tatatgaagg aatcactttg
    27541 ggtaaacaag gaacagtcgg cttgattaca tacatgcgta ctgattctac ccggattgcc
    27601 gatacagcaa aagcagaggc cgcagcattt attgaagata cgtatggaaa agaattttct
    27661 agacatgatc accgaaagat caaaaatgct caaggcgcac aagatgccca tgaagctgtg
    27721 cgtccaacga gtgtgcttcg tacaccagat gaactcaaac aatatttaga taaagatcaa
    27781 ttgaaacttt atacattgat ctggtcacgt tttgtggcta gtcagatgac accagctatt
    27841 ttagatacaa tgaaagtgac attagaacaa aatggtgtca tcttcattgc gaacggttca
    27901 aaaatcaaat tcaaaggatt catgcaagtg tatgtggaag gtagagatga cggcaaagaa
    27961 gagaaagaaa atattcttcc agaacttgaa gaaggcgatg tagtccaatc tgttgatatt
    28021 gaaccaaaac aacattttac tcaaccacca gcacgtttta gtgaggctac gttgattcgt
    28081 gcactagaag aaaacggggt agggcgacct tccacttatg caccaacact tgatacgatc
    28141 caaagacgct actatgtaaa attgacacaa aaaagatttg agccgactga actaggagaa
    28201 attgtcaatt cattaatctg tgaattcttc cctcaaatcg tcgatatcca tttcacagca
    28261 gaaatggaag gagatttgga taaaattgaa gaaggcacgg aagcgtgggt caaagtggtt
    28321 gatcgtttct ataaaccgtt tgaaaaagaa ctgacgaatg cggaagaaaa aatcgaaaaa
    28381 attcaaatca aagatgaacc agcaggattc gattgtgatg tctgtggaca tccgatggtg
    28441 ataaaattag ggaaatatgg gaagttttat gcttgcagta attttcctga ttgtcggaat
    28501 acgaaaccaa tcgtaaaaga aatcggtgtg acttgcccag tatgtcatga aggacaagtc
    28561 attgaacgaa aatctaagaa aaacagacta ttctatggtt gttcccgttt cccagaatgt
    28621 gaattcactt cttgggataa accagtgggc agaaattgtc caaaatgtga cggatatctg
    28681 gtcgagaaga aagtcaaagg cggtaaacaa gtagtctgtg taaatggaga ttacgaagaa
    28741 gacatccaaa aataatagaa aagaggttct ttcattgaaa atggtaaatg ttgttggagc
    28801 agggcttgcc ggaagtgaag ctgcttggca gatcgcacaa gcaggagtcc ctgtccgttt
    28861 atatgaaatg cgtccagtca aatcgactga ggcgcatcac acaagtaatt ttgctgaact
    28921 agtatgttcc aattcattaa gaggaaacag tttggctaat gcagtagggt tattgaaaga
    28981 agaaatgcgc cgattgaatt cggtagtaat cacttcggca gatgaaacag cagtgccagc
    29041 tggtggtgcc ttagctgttg accgcgattc tttttctgaa acaattaccc gaaaagtcaa
    29101 agagcatcca ttagtaacag tgataaacga agaaattact gaaataccgg aaggaatcac
    29161 cgtagtagca acaggacctt tgacttctca tccattagca gaaagcatca aagcattcaa
    29221 tggttcagat ggtttttatt tctatgatgc agctgcacca atcgtagaca aagctacgat
    29281 cgatatggat aaagtatatt tgaagtcccg ctatgacaaa ggagaagcag cttacttgaa
    29341 ctgtccgatg acagaagagg aattcaaagc tttccatgag gcactaatca atgccgaagt
    29401 ggcagaactt aaatcttttg agaaagaaaa atattttgaa ggatgtatgc caattgaagt
    29461 aatggcacag cgaggattca aaacgatgct tttcggacct atgaaaccag ttggattgga
    29521 agatcctaaa actggcaaac gtccttatgc tgttatccag ctgcgtcaag acaatgctgc
    29581 agcttcttta tacaacatcg tgggctttca gactcacttg aaatggggcg agcaaaaacg
    29641 tgttttccgg atgattcctg ggttagaaaa tgctgaattc gttcgctatg gtgtgatgca
    29701 ccgaaacagc tttatgaatt cgccagaatt gctgaaaccg acttaccaat caacgaaacg
    29761 cgaagatctg ttctttgcag gtcagatgac tggtgtagaa ggctatgttg aaagtgcagc
    29821 gagcggacta cttgctggaa tcaacgccgc acgcttagca aaagaaatgg aaccagtcga
    29881 gtttccacaa gaaacagcta ttggaagtat ggcttactac attactcatg cggaaggaaa
    29941 acatttccaa ccgatgaatg ccaactatgg tctgtttcct gaactgccag agcgcattcg
    30001 agacaaaaaa gcaagatatg aagcaattgc acaacgagct ttaaaagcta atgaggcaat
    30061 aaaaacagaa ttagaaaaaa ctgcaatccc aaatgagtaa ggaaactcca tgaagagggt
    30121 gagacggata gtctcgctct ttttcttgcg tttttcttaa gaatttctca aacaaaatgt
    30181 gaggaattgc gccaattgtg aacaaactta gaaatattgg ttaaattctg acaatttatt
    30241 tgtcttttta taaacgctgt gctaaattat tctctgggac aaaaggaagg tgagaaaatg
    30301 acagaagcca cgtgggaaca aaaatttttg cgttatttga ttgtcgaaag aggatattca
    30361 gaaaaaacga aagaagcata tgaagaagac attctccatt tcaaacattt tttggaggaa
    30421 tctggagacg ctgatctgct gaaggcggaa tattttacgg ttcgtgtcta tttgagtgca
    30481 ttagctgatc atcgtcctag ctatagcaga aattctatca gtcgcaagat agccagccta
    30541 cgttcgtttt atcagtatct tctcaaagaa gaagtgataa aagaaaatcc attttcttac
    30601 gttcatctca aaaagaaaaa tttgagactg ccacgttttt tttatgaaaa tgaaatgcaa
    30661 gcactttttg acagcgtagc aggagatagt cctttggatt tgaggaaccg cgctttactg
    30721 gaagtccttt acgggtcagg catccgttta agtgagtgta gcagtttgtc ggtatcagac
    30781 attgattttg attcagaggt catgttgatc catggtaaag ggaacaaaga gagatatgct
    30841 cctctaggtt cttttgctca agatgctctg caagaatatt tcagggaagg ccgccaggta
    30901 ttgatgacaa aatatcacga agagcatgat atcgtttttg tgaatcatca tggaaaacca
    30961 atcacgccta ctggaattga atatgtcttg aatcaaatga tcaaaaaaag cagtttggat
    31021 agtgatatcc atcctcacat gctgcgccat acatttgcta ctcatttatt aaataatggg
    31081 gcagatttgc ggacagtcca agaattgcta ggacatgcca atttatcgac tacccagatc
    31141 tatgcccatg taacaaaaga aagtttacaa aaaaactatc gttctttcca tccaagagcg
    31201 taaaaataag gaaaagaaca aatttaggag gaaacaaaca tggtcgaatc accatttcat
    31261 tcaaccacta tctgtgccgt cgaaaaagac gggaaatttg ctatggctgg agatggccaa
    31321 gtaacaatgg gtgaatctgt tgtcatgaaa ggcactgcaa agaaagtacg acgtatctac
    31381 aatggagaag ttgttgtagg tttcgccgga agtgttgcag atgcgtttac tttagaagaa
    31441 aaatttgaag gcaaactaaa cgaatataac gggaatctac aacgcgcagc tgtcgaatta
    31501 gctcaagaat ggcggacaca gcaatctatg caaaaacttg aagcgatgct gatcgtaatg
    31561 aatgataaag aaatgcttct tgtttcaggt acaggagaag ttatcacacc agatgatggt
    31621 atcttagcta tcggatcagg agggaatttt gcgttggctg cagcccgagc aatgaaaaac
    31681 tatgctcaaa acgacatgag tgcgaaagaa attgcagaaa atgcgttaaa tattgccgca
    31741 gatatctgta tctttactaa tcataatatt atcgtagaag aactgtaaga ggtgagtaga
    31801 tatgtatgaa ttaaaacaaa caccaagaca aatcgtaagt gaattagatg aatatatcgt
    31861 aggacagcac gcagcaaaaa aatctgttgc cgttgctttg cgtaaccgct atcgccgttt
    31921 gcagttagat gaaaaaatgc aacaagatat tacaccaaaa aatatgctga tgatcggacc
    31981 tacaggggtc ggtaaaacag aaatcgcacg acgtttggca aaaatcgtca atgcaccatt
    32041 tgtcaaagtc gaagcaacaa aatttactga agtcggttat gtcggaagag atgtagaatc
    32101 aatggttcgg gatttagtag aaaatgcgat ccaaatcgta gaaaaagagc agtacagccg
    32161 tgtatacaca agtgctaaaa aaaatgcaga aaaacgtttg gtaaaaatct tggtacctgg
    32221 tatcaaaaaa gaacagaaac aatccactaa tcaatttgaa caaatgatga atctttttaa
    32281 tgctagtcag caaaataccg ctgaaccgca agaagaagtg acagaagata ttcgaatcaa
    32341 tcgccgtaca gtcttggaac aactacaaaa gggtttactg gatagtcgtg aagtaaccat
    32401 tgaagttgaa gaccaaaaga aacctgcacc aatgatgaac aatggattgg agcaaatggg
    32461 gatcgaccta aatgaaactc taggtgcttt gacaccgaag aaaaaagtgg aacggacggt
    32521 aacagtcaaa gaagctttag agttattgat caaggaagaa tcagcaaaat tagtcaagga
    32581 tgctgatatc catagtgagg caatcaaatt agcggaagaa agtggaatta tttttatcga
    32641 tgaaatcgat aagatcactt ctaaatcaca gcaatctgga gaagtatcca gagaaggcgt
    32701 ccaaagagac atattgccaa ttgtcgaagg ttcacaagta aacacaaaat acgggatcat
    32761 ccaaacagat cacgtcctat tcattgcttc aggagctttc catttgtcta aaccaagtga
    32821 tctaattcca gaacttcaag gacgcttccc gatacgggta gaattagatg atttgacagc
    32881 tgaagatttt gtgaagattc tgaccgaacc aaataatgct ttagtaaaac aatacgtcgc
    32941 gttgatcggt acagaaaatg tgtcagttac gtttacaaaa gaagcaattg aacggatcgc
    33001 tcaaatcgct ttcgatgtca accgtgacac agataatatc ggcgcacgca gactgcatac
    33061 cattttggaa aaactactag aagatctatt atttgaagca ccagatatgc aaatgggaga
    33121 aataacgatc acagaagcat atgtggatga caaacttggc tcaattgcgg caaatgaaga
    33181 tttaagtcgc tatattttat aaaaaatcgg aggacgacaa tgacagactt gctaacgaaa
    33241 acccgcaaga taaacacact tttgcagcaa aagaatactt ttgacatgca agcagatttg
    33301 ccttacgata aaatggctgt cacactaggt ggtattcttg acagcaacac ctatatcata
    33361 ggaagtgacg gagtattact aggttataat gaacgtcacg atgtgaacaa tgaacgaatc
    33421 aaaacaatgt ttgttgagaa acaatttcct gaatcttata ctgatacagt ggcacagttg
    33481 atgaaaactg aagctaatat cccaattact agtgatatta ctgcatttcc ggtagagtca
    33541 agagagaaat atccatttgg tctgacgact gtcgttccga tctttggagc tggcgaacgt
    33601 ttgggtacga ttatcttatc tcgtatggaa caatcgtttg cagatgacga tctagtctta
    33661 gcagaatatg gagctacggt tgtcggtatg cagatccttt atcagaagtc tcgaagcatc
    33721 gaagcagatg tacggagtgc aacggctgtc caaatggcaa tcaacacatt atcttatagc
    33781 gaattgaagg ctgtacaagc tatctttgaa gcattggatg gagatgaagg tcgtctgact
    33841 gcttctaata ttgccgatag tatcggcatc actcgttctg tgatcgtcaa tgctttacga
    33901 aaattggaat cagctggaat cattgaatct cgttcgttag gcatgaaggg aacttattta
    33961 aaagtattga attcccgttt taaagacgag ttagcaaaac acagttatta gaagtaaggg
    34021 gactggggca gatagaaatg tttgttccag ccttcccatt ttaaaaagta agtcgaagtt
    34081 ggaggaatgg caatgactgt tattattcaa aatgaccagc tgatcgccga aatcgctgaa
    34141 cacggtgcgg aattgatcag tctgaaatca aaagaacatg gtttagagta tatctggcaa
    34201 ggtgatccag cgtattgggg cagacatgca ccggttttat tcccttttgt tggacgttta
    34261 aaagatgatc agtatgttta tcaagggaaa acatatcaaa tggggcaaca cggatttgct
    34321 cgggatatgg attttgaagt cattgaacat ggacaagaaa tggctagttt catcttaagg
    34381 agtacgccgg agacattaga aaagtatcca tttgattttg aattagtgat cagctatgaa
    34441 ttaggaggag acggcatcac ggttcattat caagtggcaa ataccggtga aaaagaaatg
    34501 ttcttttcta tcggtggaca tcctgctttc aatgtacctc ttgaagaagg attgtcattt
    34561 gaagatttct atctggcatt ttctccattg aaatcaagat tacgcctacc attgaaaggc
    34621 ccattcatcg atatggatca aaagacgtta ggtcagacaa atacgaatct ggctttgaat
    34681 cgtgaacttt ttgagcaaga tgccttggtt tttgaaacaa ggggtctaaa tgcgtacgcc
    34741 attcgaagtg aaaaatcttc ccatagcgta actttaagct ataaagatat gccttatgta
    34801 gggatctggt ctccttatcc aaaagaagcg cctttcgttt gtatcgaacc gtggtgcgga
    34861 atcgctgaca cggtgaacag tagtggaaac ttgatggaaa aagtagggat caatcaacta
    34921 ggtgcccatg aaatcttcaa gacaaaatat tccatcacag tgaagtaaga ggttgtgacc
    34981 gagaagttag cagggaacac cgtttaccaa ggtgtgtaaa aaagctatcc tgtaagtaat
    35041 aagggagtgt gacatcgatt tgtcacactc ccgttttatc tctttggaat gaaaaagtat
    35101 caggattttt tcttgctgct attcaaacca aacgggattc gactttcagt atggtttctg
    35161 atacgcttga tattgtcttt atgccgataa aagataaagg aagaaactaa aaaggcaatg
    35221 atcgtcagta accaattggg cttagccaaa atcacaggag ctacataagg aaggacgatc
    35281 gtagataaag tgatcaatac tgcactgatc atactgctca aactcaccat acttgtacaa
    35341 aataaactaa cgataaagat gatacaagaa taaacaaaaa atacaggatt aaatcctaaa
    35401 agcattcctg cacttgtcgc aacagctttt cctcctttga acccagcaaa aatcggaaat
    35461 gtatggccaa gaatggcgca taccccaaac caaagagggt taatcccaga aaggtggaag
    35521 atcatcggca aacaagtagc caatgtacct tttaaaatat ccataagcag gacaacaatc
    35581 ccggctgttt tgcctaatac acgaaatgta ttggtcgttc cagtattacc gctcccgtgt
    35641 tgtcggatat cttttttgaa aaacaatttg ccaatccaga caccggaagg aatagaacct
    35701 aaaagatatg ctaaaataag taataagatc aatttcattt tttaccctct ttcaagttga
    35761 aaactatcct ttattttagc atgaataggt aacaggaaca attgaaaaaa tgaaagatac
    35821 aagtataata gatggaataa aaagaaaagc aaggggtggc agaaatgtct gtaaaaaatc
    35881 caacacaaga aacgattttc aactatttgg aagaagcaaa aaatattgcc gtcgtaggct
    35941 tgagcaataa accagaacga acaagttaca tggttgcaca ggtattgcaa gacaatggat
    36001 ataaaatcat accagtaaat ccattattgg ctggtgaaga aattctaggc gagaaagtct
    36061 atgcgaattt gacagaaatc gatgatccaa tcgatatcgt agatgtattt agacggagtg
    36121 aatttttacc tgaaatagca ggtgattttt tgaaaactga tgctaaagtg ttctgggcac
    36181 aattagggat tgaaaacaaa gaagcagctg agatgctaag aaaagcagga cgtacagata
    36241 tcgtaatgga tcgttgtatt aagattgaac ttggtaagtt gaaagaaaac caaaaataaa
    36301 aatgaccaca aagaagagat gatcgataga ggggaatgtc gaaaaagcgg ttaggagata
    36361 attaaaattg atttctcgtc taccctcttt tttttcacga ccttttctag tatgataatc
    36421 gtggtgcggt tttgccgcaa agtattattg aaggagcgtt tgccttggca aaaaaagtaa
    36481 acaacgaata caatgactcg tccatccaag tattggaagg attagaggca gttagaaaaa
    36541 gaccagggat gtacattgga tccactgaca gccgaggctt gcatcattta gtctatgaaa
    36601 tcgtcgataa tgcagttgac gaagcattat caggttatgg cagtgagatt gatgtgacca
    36661 tccatgaaga caatagtata actgttgcag atagcggacg tgggatgcct gtaggaatgc
    36721 atgcttccgg aataccaaca gttgaagtga tttttactgt gctgcatgct ggcggaaagt
    36781 ttggtcaagg agggtacaaa acttctggtg gactgcacgg tgtaggagcc agtgtggtaa
    36841 atgccttatc gaaatggcta attgtaacga tcgttagaga tggcgtagag tatcagcaga
    36901 aatttaaaaa cggcggtaaa cctgacggta ctttgaaaaa gataggaaaa acaaaaaaag
    36961 caaacgggac gactgtccat tttttaccag atgatacaat attcagtaca acgaaatttt
    37021 catatgaaat tttagccgaa cggttaagag aatctgcctt tcttctaaaa ggcgttaaaa
    37081 tctctctttc tgatcttcgt ggagaagaac cagtcaagga aatttttcat tacgaagaag
    37141 gaatcaaaga atttgttgat tatctgaatg aagaaaaaga tacattgaca ccagttgttt
    37201 atttttcagg agaaaaagaa ggtatcgaag tagaggtcgc ttatcaatac aatgacggtt
    37261 attcagaaaa tgttttatct tttgtcaata acgttcggac aaaagatggg ggaacccatg
    37321 aagctggtat gaaagcggcg atgacgaaat cttataatga atatgcgaga aaagttggtt
    37381 tgctgaaaga acgggacaag aatctggaag gtagtgattt cagagaaggg ttagctgctg
    37441 tactttctat ccgtgtacct gaaaatcttc tgcaatttga agggcaaaca aaagaaaaac
    37501 ttggtacgcc agttgctcgt accgtagtag ataacgtcat cagtgaacaa atgggctttt
    37561 atttgcagga aaatagcgaa atgagccaga tgttggtccg taaagcaatc aaagccagag
    37621 aagcgagaga agcagcacgt aaagccagag aagaaagccg aaatgggaag aagcgtaaaa
    37681 aaggcgaatc tcttctttca ggaaaattga cacccgcaca atcccgaaac cctaagaaaa
    37741 atgaattgta tttagtcgaa ggggattctg ctggcggatc tgctaaacaa ggaagagaca
    37801 gaaagttcca agcaattcta cctttacgag gaaaagtcat caataccgaa aaagctaaaa
    37861 tgcaagatat cttgaaaaat gaagaaatca atacgatgat ctatacgatt ggtgcaggag
    37921 tcggtcctga gttttctatc gaagactgta attacgacaa agtaatcatc atgaccgatg
    37981 cggatacaga cggcgcccat attcaagtat tactgcttac tttcttttat cgttatatga
    38041 aaccgctgat tgaagctggg aaagtatata tcgcgttacc gcctttatat aaagtatcca
    38101 aaggacaagg aaagaagcaa gtcattgaat atgcgtggac agatgacgaa ttggcagcaa
    38161 tgattaaaaa agtcggcaaa ggatatatgc tgcagcgata taaaggactt ggagagatga
    38221 atgcggaaca attgtgggaa acaacgatgg atccgacttc ccgtacgttg atccgcgtcc
    38281 gtatcgatga tgctgcgcaa gctgaacgtc gagtaaccac actgatgggt gacaaagtag
    38341 aacctcgaag aaaatggatc gaaaatcacg tgcaatttac gctcgaagaa gatggcagta
    38401 tcttagataa aaaagaggat actgaaatct ctccttcagt ttctaatgat ttattagatg
    38461 aagaacgagc agataaaaat gaaaacaatc agttatttga agtagaatag gaggtcacct
    38521 gatggaaaat cgacaagaag tacaagaatt aactttagaa gaagtgatgg gtgaccgctt
    38581 cggacgttat tccaaatata ttatccaaga acgtgcactg cctgatatcc gtgatggatt
    38641 gaagccagtg caacgccgga tattatttgc catgaacaaa gatggcaata cgtatgacaa
    38701 aggattccgt aaatcagcaa aatctgtcgg aaatatcatg gggaattatc atcctcatgg
    38761 tgacatcagt atttatgaag cgatggtccg catgagccaa gactggaagt tgcgggaagt
    38821 actgatcgaa atgcacggga ataacgggag tatggacggt gatccacctg ctgcgatgcg
    38881 ttatactgaa gccagattat ccaagttaag tggagagatg ctagcagata tcgagaaaga
    38941 aacagtcgat ctcgtgtgga attttgatga tacagaaaaa gaaccaacag tccttccagc
    39001 tcgataccca aaccttctcg tcaatggatc gacaggtata tctgctggat atgcaacaga
    39061 aatcccgact cataacttag ctgaagtgat cgatggcaca atctatatga tcgatcatcc
    39121 acaagcgagc cttgaaaaat tgatggaata tattcctgga cctgactttc caacaggagg
    39181 gatcttgcaa ggaaaagatg aaatcaaaaa agcttatgaa acaggacgag gaaaagttat
    39241 tcttcgttcg aaaacaaaga tagaggcaat gaagggtaat aaacagcaaa tcgtgatcac
    39301 agaaatccca tatgaagtaa ataaagcgac cttggtgaaa aaaatggatg agatccgttt
    39361 gaataagaaa atcgatggaa tagctgaagt acgagatgaa agtgatagaa caggtctgca
    39421 aattgtcgtc gaattgaaaa aagacgccaa tgctcaagga attttgaatt atttgttcaa
    39481 aaacactgag ctacagatca attacaattt caatatggtc gctatcgacc atatgactcc
    39541 gcatcaagtt ggactgaaag acattttaag aagttatatc gagcatcgaa agcaagtcat
    39601 aacaaaacgt agtcaattcg atttagccaa agctcaaaaa agacagcata tcgtagaagg
    39661 attgatgaaa gctttatcga ttctggatga agtaatcgca acgattcgtg aaagtaaaga
    39721 caaaaaagac gccaaaaaga atttagtcga cgtctttcaa tttacagaag aacaagcaga
    39781 agcaattgtc acattgcaat tataccgttt gacgaacacg gatatcacag agcttcaaaa
    39841 ggaatccgaa agcttgattg cgcaaatcac agagttgaat aaaatccttt caaatgacaa
    39901 agagcttttc tctgttatga aaaaagaatt gcgagaagtg aaaaaaaatt attcttccgc
    39961 tcgtctaacg acaatcgaag acgaaatcga agaaatcaaa attgatacgc aagtcttagt
    40021 ggcacaagaa gacgtaattg tcagtgtgac gcgagaaggc tatgtgaagc gaacaagcct
    40081 tcgttcttat agtgcttcca aaccagaaga aatcgggatg cgagaaggcg actacttgct
    40141 atactcaggt gaattgagta cattggacca tgttcttctg atcacaaata aagcaaatgt
    40201 gatctaccgc cctgtccatg aactacccga tttgaaatgg aaagatgctg gtgagcacat
    40261 ttctcaaaca atctccaatc tgtctgtaga cgaatcgatt ttagctgttt tcccatatca
    40321 aaaaattcaa gcagagaaaa catttgtatt catcagcaag aatggattga tcaagcaaac
    40381 acgaatgaca gagttcgaac catggcgcac ctataagagc cgtccgctca gctgtatgaa
    40441 gctaaaggcg gaagaagatg aactgattgc tgtgtatctg aaagaagatc agccaggctt
    40501 agatgttttc ttagtaacgc atcaaggaat gggtttgcgc tatcctttaa cagatgttcc
    40561 cgttgttgga gcaaaggcag ctggagtgaa gtctatgaat ttgaaagatg gagataggat
    40621 tatcaacggg ctgcttgtgt tagcagaagg tgatacgccg atcgtaatcg ttactcagcg
    40681 aggagctgtt aaacgcatgc ttgctcaaga aatcagccaa acttcacgtg cgaagcgggg
    40741 agtgacggtc atgcgtgagc tgaaaaaaaa tccgcaccgt attatctata tgtcggaagg
    40801 aagatcaaaa gaaataacct tgatgaatca aaaagggcag cagttaacga ttgacccaac
    40861 tgatttccct ataggtgatc gaacatcgaa tggatcattt gcgatggatg agaaaaaagg
    40921 tggagaagtc atcgaagtta tcgatgctcc tgagattttc atcgagtcgt aactgttgta
    40981 gtattttaat gaagagacgc catctgtatt aatacaggtg gcgtctcttt tt
//
LOCUS       DADVXU010000290        30589 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.guided.022, whole genome shotgun sequence.
ACCESSION   DADVXU010000290 DADVXU010000000
VERSION     DADVXU010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 30589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.guided.022"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            1246..1560
                     /locus_tag="KB953_003066"
     CDS             1246..1560
                     /locus_tag="KB953_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867947.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFQSFRNQIIRSGKT
                     VN"
     gene            complement(1613..2386)
                     /locus_tag="KB953_003067"
     CDS             complement(1613..2386)
                     /locus_tag="KB953_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867948.1"
                     /translation="MKKFAIILTCALLIGTISGCTTSDTSSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSETESTEKILPIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKVNIEKGALAQLYTEKVEQFFEAGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMKLKKIKSYDYPLNLTLNKGILSFVLAGYVDYEYTADYLETPLQHDNNDINFR
                     FIYNSKSKTWLVDQLSTINTGFHELPKPDNETALVIKTF"
     gene            2491..2736
                     /locus_tag="KB953_003068"
     CDS             2491..2736
                     /locus_tag="KB953_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBB6867949.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRVRLEKGIGQTELIRDLQLRKIA
                     ITRETLVKIEGGRQHIKLDQLKAIKDILQVSYEDLLQ"
     gene            2892..3743
                     /locus_tag="KB953_003069"
     CDS             2892..3743
                     /locus_tag="KB953_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HBB6867950.1"
                     /translation="MYKAYELDFTNADLNAFSSSSQSYTHVVNQIDHNQKSINKRIEN
                     LFESENDLVDYYSKDSKILDADEIIKDWFPTIKADIFISHSHADEKLAIRFASWLFEN
                     FGLTAFIDSSVWGYSSDLLKKIDQKYCYKEQTKTYDYDKRNVTTSHVHMMLSTALNNM
                     IDSTECLFFLNTPNSISLSNEITNEQKFTYSPWLYSELTTASIVEKKNPRLESNPQMS
                     TEDVRSIIKHYSSQENLQIKYSLNLSELTYLRKADLKKWALDAVECTKGSNTLDILYK
                     NTIFTNL"
     gene            3776..4450
                     /locus_tag="KB953_003070"
                     /pseudo
     CDS             3776..4450
                     /locus_tag="KB953_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287699.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
     gene            4707..4895
                     /locus_tag="KB953_003071"
                     /pseudo
     CDS             4707..4895
                     /locus_tag="KB953_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604486.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            4941..5906
                     /locus_tag="KB953_003072"
     CDS             4941..5906
                     /locus_tag="KB953_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6867951.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            5928..6140
                     /locus_tag="KB953_003073"
     CDS             5928..6140
                     /locus_tag="KB953_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HBB6867952.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            6215..6691
                     /locus_tag="KB953_003074"
     CDS             6215..6691
                     /locus_tag="KB953_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HBB6867953.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            6688..8496
                     /locus_tag="KB953_003075"
     CDS             6688..8496
                     /locus_tag="KB953_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HBB6867954.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            8573..8788
                     /locus_tag="KB953_003076"
     CDS             8573..8788
                     /locus_tag="KB953_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family protein"
                     /protein_id="HBB6867955.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            8880..9743
                     /locus_tag="KB953_003077"
     CDS             8880..9743
                     /locus_tag="KB953_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867956.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            9780..10316
                     /locus_tag="KB953_003078"
     CDS             9780..10316
                     /locus_tag="KB953_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HBB6867957.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            10330..10752
                     /locus_tag="KB953_003079"
     CDS             10330..10752
                     /locus_tag="KB953_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HBB6867958.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            10682..13081
                     /locus_tag="KB953_003080"
     CDS             10682..13081
                     /locus_tag="KB953_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HBB6867959.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            13113..15104
                     /locus_tag="KB953_003081"
     CDS             13113..15104
                     /locus_tag="KB953_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBB6867960.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            15127..15378
                     /locus_tag="KB953_003082"
     CDS             15127..15378
                     /locus_tag="KB953_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HBB6867961.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            15368..16597
                     /locus_tag="KB953_003083"
     CDS             15368..16597
                     /locus_tag="KB953_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HBB6867962.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            16594..18675
                     /locus_tag="KB953_003084"
     CDS             16594..18675
                     /locus_tag="KB953_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HBB6867963.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            18824..22744
                     /locus_tag="KB953_003085"
     CDS             18824..22744
                     /locus_tag="KB953_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HBB6867964.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKTD
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLIERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            22745..23689
                     /locus_tag="KB953_003086"
     CDS             22745..23689
                     /locus_tag="KB953_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867965.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            23693..23896
                     /locus_tag="KB953_003087"
     CDS             23693..23896
                     /locus_tag="KB953_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HBB6867966.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(24196..24432)
                     /locus_tag="KB953_003088"
     CDS             complement(24196..24432)
                     /locus_tag="KB953_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBB6867967.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(24615..25001)
                     /locus_tag="KB953_003089"
     CDS             complement(24615..25001)
                     /locus_tag="KB953_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HBB6867968.1"
                     /translation="MDARAMSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECI
                     CVQSISYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAA
                     CSKRMRRRKEAERQRKRYWKPTHLEL"
     gene            complement(24983..26311)
                     /locus_tag="KB953_003090"
     CDS             complement(24983..26311)
                     /locus_tag="KB953_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBB6867969.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(26272..26601)
                     /gene="mobC"
                     /locus_tag="KB953_003091"
     CDS             complement(26272..26601)
                     /gene="mobC"
                     /locus_tag="KB953_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HBB6867970.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(26859..27229)
                     /locus_tag="KB953_003092"
                     /pseudo
     CDS             complement(26859..27229)
                     /locus_tag="KB953_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            27374..27559
                     /locus_tag="KB953_003093"
     CDS             27374..27559
                     /locus_tag="KB953_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBB6867971.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            27717..27884
                     /locus_tag="KB953_003094"
     CDS             27717..27884
                     /locus_tag="KB953_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867972.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            28071..28289
                     /locus_tag="KB953_003095"
     CDS             28071..28289
                     /locus_tag="KB953_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6867973.1"
                     /translation="MRQERPEGVHTGPACVKSHTARCIRKKEAAHFAEAWGVPNKQNE
                     NAAGGQVVDQNIRKGYSDPLLASIIGMF"
     gene            28502..29161
                     /gene="vanR"
                     /locus_tag="KB953_003096"
     CDS             28502..29161
                     /gene="vanR"
                     /locus_tag="KB953_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HBB6867974.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            29164..30525
                     /gene="vanS"
                     /locus_tag="KB953_003097"
     CDS             29164..30525
                     /gene="vanS"
                     /locus_tag="KB953_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HBB6867975.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLT
                     STL"
BASE COUNT         8158 a         7547 c         7915 g         6969 t
ORIGIN      
        1 tatcaccgaa gtgacgaatg aaactatgac caaattaagc aagaaaatat atcttgcaaa
       61 taaagtagta taaaaagata ttgataaagc caatcaaaac tttgaatttc aaacgttaga
      121 taatcaaaca gatacaaaaa atgaaatcag ttctacgcgt acgattatta aaaataataa
      181 attttcacta gaagaaaatg gaaataaaat caaataatta ggaagaaaaa acaaagcaat
      241 tagaaaagcc tgcaagcaaa tttgagtaat cgagatccaa tttttttaag gaagtttaac
      301 aaaaatatgt aattctatta tcactattaa agagaaagtt ggagcggaat ataaagaaaa
      361 gagaaaagta attaaacgca aaaaaatgta gaacttaaaa aatataaaga ggaacaaccg
      421 ttaaaattag gaatgatgtt gtgtaaacat tataattgct atgatataaa tgaagtgtta
      481 aagaaagcaa atttaaacca attttgtgaa cagaaaagcc ttagtcacta tgaccaaaac
      541 tacggacgaa actatgacca aaagtaggta ctaaattatg accgaaaata ggtccaaaat
      601 ggattttttt atgatatcaa aaattatgaa atgattttga aagaaaacca gttggcacag
      661 ccaacttagc caccttcctg taggaaggtg ctatcagttt tcccttatgc tcttttcttt
      721 cttttttgtt tcatatgtca aatgccattt tgaaatgtac catcttaaga aatttatcct
      781 taaaaacaat tgcgttattg gataaaaaga tgctacatta tcttaaaaaa ggaatcaaat
      841 atgatgaatt tagtggctag catatcgtgt gattaagata ctttatagtg taagttgaag
      901 gagaaaaaat atttaaaata tacttcccat ttcagtggga agtaaaatta atcttgttgt
      961 ttctactagt aatgtatctt tttggaaact atgtaacgat gtaaacaaga tttttttcag
     1021 aagaaaaaac gataaaagtt gtgataaacg atttaaaaaa taggagaaaa tatatcaagc
     1081 gcatggtatg aaacgcacat ttaaaaagat tcgactaact aaaaaaataa gtttacaaag
     1141 cattgttgtg tattacaagt gatctataca ctgaaaaatg atagagaaaa tctgatacgt
     1201 ccgcaacaat ggacaattga aaatgaaagt gaggaaattg gaagaatggc tgatgaaaat
     1261 aaagttgttc cgcttgaaac actagtgata aataaggaag agtttgagca actttttctt
     1321 gatttttttg ttcaatatga caggatttat gattttagtt cgtcactaga tttatttgaa
     1381 ttagaggcag aaataataga aaacagtcga gaggagaatg gtaaaatcat catttcagta
     1441 gaaaaattca atgaattatt ctcagaaaca tttgaaatag atacagatta ttatcaacca
     1501 caagaagttt tccaatcatt tagaaatcag attattcgct caggtaagac cgtaaactga
     1561 aaaacaataa aagaaacgtt gaaagaataa ttattccttc aacgtttctt ttttagaatg
     1621 ttttaatgac caaagcagtt tcattgtcag gcttaggtaa ttcatgaaat cccgtattta
     1681 tagtgctcaa ttgatcaact aaccatgttt tactttttga attgtagata aatctaaaat
     1741 taatatcgtt attatcgtgt tgaaggggcg tctccaaata atctgctgta tattcatagt
     1801 caacgtaacc tgctaataca aaagagagta tccctttatt taatgttaga tttaatggat
     1861 aatcatacga ctttattttt ttcaacttca tattgtacat atcttcagcc ataaaagttg
     1921 agttcgtgat ttcatcttgg gtctcattaa aaaagttatc cgaataatcg cttatttttg
     1981 aaaagtcttt attggcacca gcctcaaaaa attgttcaac tttttcagta taaagttgtg
     2041 ctaacgctcc tttttcaata ttaactttaa tattttcatg tctatcaatt tgtgtgctgt
     2101 tcagtaaggg aagatcagct tttagtttta tatcgtcttg atttctttca atttcaattg
     2161 gcaaaatctt ttcagtagat tcagtttctg ataaaggaac atctagtttt ttatcatgat
     2221 aataagcttc aaatccatct ggaaccggaa aaaaatgtat tgggtaattc ttttctgagg
     2281 ttgttgtact agtttccgta cttgtagaac ttgtgtcaga agtggtacac ccgcttatag
     2341 ttccgatgag taaagcgcaa gttagaatga ttgcaaattt cttcaaaaaa attcctcctt
     2401 aaaattgaat atggactaat actagtccca ttgtagcata ccgaatcttt aagattaata
     2461 agaaaaagga ctaattttag tccaaaaggt gtgctattct tgaaagagga aaaaattatt
     2521 gataaagaaa atgtaatcgg tagcaatatt agacgagtcc gattagaaaa aggaattggt
     2581 caaaccgagc tgattagaga cctacagctt cgtaaaattg ctataactag ggaaacgttg
     2641 gttaagattg aaggtggccg ccaacacatt aagttggatc aattgaaagc catcaaagat
     2701 attctacagg tgtcttatga agatttgctt caatgactac ttgtagtcat ttcgtttgaa
     2761 attattatgt tacaccctta tgtgtcacga agaaatactg gtataataat gaatgggata
     2821 tgtaacgata gggtatactg ttatgttgcc taataaattg tggatcaaaa tacaagaatg
     2881 gagggatatc cttgtataag gcatatgaat tggattttac aaatgctgac ttaaatgcat
     2941 tctcctcaag ctcgcaatca tatacacatg tagtaaatca aatagatcac aaccaaaaat
     3001 ctattaacaa gagaatagaa aatctatttg aaagtgaaaa tgatttagtt gattattatt
     3061 ctaaggacag caaaattcta gatgcagacg aaattattaa agattggttt ccaactataa
     3121 aagcagatat ttttatatct cactctcatg ctgatgaaaa attagctata cgttttgcat
     3181 catggctatt tgaaaatttt ggtcttactg catttattga ttcttcggtt tggggataca
     3241 gttctgattt attaaaaaaa atagaccaaa aatattgcta taaggaacaa acaaaaacat
     3301 atgattatga taaaagaaat gtaacaacta gtcatgttca tatgatgctt tctacagctt
     3361 taaataatat gattgatagt acagaatgtc tgttttttct taatacacct aattcaatta
     3421 gtttgagcaa tgaaatcact aatgaacaga aatttacgta ttcgccatgg ctatatagcg
     3481 aactgacgac agcttcaata gtggaaaaaa agaatccacg attggaaagt aatcctcaaa
     3541 tgagcaccga ggatgttaga agcattataa agcactattc ttcgcaagaa aatctgcaaa
     3601 taaaatattc tttaaattta agtgaattaa cttatttacg aaaagctgat ttgaaaaaat
     3661 gggctttaga tgctgtggaa tgcactaaag gaagtaatac attagatatt ttatataaaa
     3721 acaccatttt cactaatctc taatcaattt actgaatcga aaggaaccgg gtagaatgga
     3781 taagaaagtt ttaataaaga ggttctctga agctattgaa aaaggaaatg cagcaatatt
     3841 tgcaggtgca ggattatcga tgtcacaagg atatgtaagt tggccggaac tacttaatga
     3901 tcctgcaaca gaaatcgggt tggattctaa aaaagaaaca gacttagtta ctctggctca
     3961 atattataaa aatgaaaatg gtggatcaag aggaattttg aatcagatac ttatggataa
     4021 ttttggtgaa gaattagaaa tatctgaaaa tcatcgaata ctagcaagtt tacctattga
     4081 aacttattgg acaactaatt atgatcatct aatagaaaaa tcaatccgag aagcttataa
     4141 aaaccctcag gttaagaaaa attatactca gttagcaaca actaatccaa atgtggatac
     4201 aattgtttat aaaatgcatg gtgatataga tgatgtcagt tcgacagtaa taacaagaga
     4261 tgattatgaa aaatatgatg attcgtacgc gttatttaaa gaaactttaa aaggagattt
     4321 gttaacaaaa acttttctct ttttaggatt tagttttaca gaccctaatt tagaaagaat
     4381 tttatcagat ataagatggg ttttgagaga aaaccaaaga cctcattatt gtataatgag
     4441 aaaaatatta tagaaaaaat tttaggttat cgctgaaagc cccggaaata cgggcatttc
     4501 gcaggatatg agaactcaat ttactaccaa tttactactt tcggattgag ccgcatatat
     4561 gctgaaccat actgctaata cccgccaccc caaatacggc acatcataaa ttgaatacga
     4621 caccgtaaac gcggcgttcc tctttcccgt catgggagaa caggacgccg cttttttatg
     4681 cccaaataga gaaaggaggc gacgccatgc tatttacttc cggccctgac cgggcgtttg
     4741 aacggctgat gcaggaaaag cccggtttcg atcactacga cggcggcggg gcggacgcgc
     4801 cggaggattg cggcacctgc cgattttacc gcccggattg gaaatatcaa ttctgccagt
     4861 ttgcagagtg tccctatcag ccgggcaagc tgaccgacta tgcagcggtc aaattcacag
     4921 tgaaaggagc gaacaacgct atggcagttt tccgtgtgga aaagaacaaa ggctatacgg
     4981 tgatgtcaaa tcaccacctg cggaataaga acctgacctt aaaggccaag ggcctgctgt
     5041 cgcagatgtt gtccttgccg gaggattggg actataccct aaaaggactg tcctatatca
     5101 accgggaaag catcgacgcg atcaggacag cggtatggga gcttgaaaaa gctggatata
     5161 tccggcgtga gcagggccgt gacgcaaaag gcaaaatggc cgatatggtc tataccatct
     5221 acgaacagcc ggtattggaa aacccggtat tggaaaatcc aacatcggat aacccggtgt
     5281 tggaaaatcc gacatcggat aatccggcgt cggaaaatcc aacgcaatta aataaagaag
     5341 tacaaagaac tgacttatca aaaaaagaaa aaataattac agatggacaa agtacccatt
     5401 ccattcctat cccatccccg acccctgccc ctatggaggg cgaggcagcg gctacgccgc
     5461 cggaacggaa aaggacagga aaggatgagg ctgtacggat ttatcgggaa atcattttgg
     5521 agaacatcga gtatgcctat ctcattcagg acaggagcat tgaccgtgaa cagcttgacg
     5581 aaatcgtaga cctgatgctg gaaaccgtct gcaccgcccg aaagacaatc cgtgtcgccg
     5641 gggacgacta ccctgccgag ctggtgaaat ccaagtacat gaagctgacc ggcgaacata
     5701 tccgctttgt ccttgactgt ctgcgggaga acacaacgaa agtccgcaat atcaaacaat
     5761 acctgcgggc tatgctgttc aacgccccca gcaccatttc caactactac acggcccttg
     5821 tcgcgcacga catggcacag cccgattggg gaaagcctaa atccggcctg ccggactatt
     5881 cctgcggcga gggcgaaagc ctgtaacgag aaaaggagga cgaccctatg aagcagggcg
     5941 cattgatttt tgacgagcga acagaccgtt acgacatccg ctttgatctg gccgactatt
     6001 acggcggcct gcattgcggg gagtgtttcg acgtgatgat cggcggcaaa tggaggccca
     6061 cccgcattga aatggccgaa aactggtatc ttgtcggcat ccgggccgac gacctttccg
     6121 gcctgcgggt gcggatttga cctatatcta cctgcggcgt ctggtggttc cgccttagac
     6181 gccgcttttg actttctgac aaggagggat gcgattgcag gaagaagtta ctcaaaagac
     6241 cattgccctg tccatgaaaa cgggcaagct cacggctcaa gtgctgcaag cggcgctgaa
     6301 aaagtacctg caacaccggg ccaagggcaa aaacacgctg caccacggcc agcagagcct
     6361 aaagcagctt aaaaagcatg gggctgatct ctccaacatt gagatcaccg aggccaacat
     6421 tggagcgttc aagccatgcg ccaagaaata cggcgtggat tttaccttac gaaaagataa
     6481 gaccacccag ccgccccact acgttgtgat tttcaaggcc aaggatgcag ataaccttga
     6541 tctggcgttt cgggagttta ccgccaagac gctttccaag gagcagcggc cctctatccg
     6601 caaggttttg tccgctgtga agcaaaaggc ggcggcccag accaagcagc gggccaagga
     6661 gaaaatcaag gaaagggggc tggaactgtg aagcctgacg taaagaaatt gctgatcctg
     6721 aacctcccgt atctgatttt tgtgtacctg tttgcgaaat gcggggaggc gtaccggctg
     6781 gccgctggtg ctgacctgtc ggggaagctc ctgcattttg cggatggttt tgcggcggct
     6841 tttgccaacc cgctgccaag cctgcacctg tttgacctgt gtatcggtat cgccggtgcc
     6901 gtgatcgtgc ggctggtggt gtactgcaag agcaagaacg tgaagaaata ccgcaaaggc
     6961 atggagtatg gcagcgcccg ctgggggacg gccaaagaca tagcgcctta catcgacccc
     7021 aagtttgaaa ataatatcct gctcacccaa acggaacggc ttaccatgac cggcaggccc
     7081 aaagacccca agacagcgcg gaataaaaac gtgctggtga tcggtggcag cggttcaggg
     7141 aaaacgagat ttttcgtgaa acccaacctg atgcaatgct ttccgaccac ggactatccg
     7201 acttcgtttg tggtgacaga tccgaaaggc actttggttc tggaaaccgg caggatgttc
     7261 cagcaggcgg gctaccgcat taaaatcctg aataccatta acttttccaa gtccatgaag
     7321 tacaacccct ttgtctacat ccattcggaa aaggacgtgc taaagctggt aaataccctg
     7381 atttccaaca ccaagggcga gggggaaaag tcagcggagg atttttgggt gaagtcggaa
     7441 cggttgtttt attcggccct gatcggctat atctggtacg aggccccagc ggaggaaatg
     7501 aacttctcaa cgctgctgga aatgataaac gccagtgaag cccgcgagga cgacccggag
     7561 tttcaaagcc ccgtcgatct catgtttgag cgtttggagc agaaagaccc cgaccatttt
     7621 gcggtgcgcc agtacaagaa attcctgctg tccgcaggca agacccgttc ttccatcctg
     7681 atttcatgcg gtgccagact tgcgcccttt gacatccggg aggtacgcga gctgatggag
     7741 gacgacgaaa tggagctgga caccatcggg gacgaaaaga cggtgctgta tctcattatg
     7801 agcgatacgg acacgacctt taatttcatt ctggctatgc tccaaagcca gctaatcaac
     7861 ctgctttgtg accgggctga tgatgtgtac ggcgggcggc tgcctgtcca tgtacgcctg
     7921 attttggatg aatttgccaa cattggccaa atacccaatt tcgataaact cattgccacc
     7981 attcgaagcc gtgaaatatc ggcctctatt attttgcaga gccagtcgca gctaaaagcg
     8041 atttacaagg acgccgccga gatcatttcg gataactgcg atagtgtgct tttccttagt
     8101 gggcgcggca aaaatgccaa ggagatttcc gatgcgctgg ggaaagaaac gattgacagt
     8161 ttcaacacca gcgagaaccg gggaagccaa acgtcccacg gattgaacta tcaaaaatta
     8221 ggaaaggagt tgatgtccca agacgaaatt gccgtgatgg acggcggcaa gtgtatcttg
     8281 cagctacggg gtgtgcgccc gtttttatcg gagaaattcg atattaccaa gcatccccgg
     8341 tacaaatacc ttgccgacgc cgacaagaag aatacctttg acgtggacag atttttgact
     8401 atcacgcgcc ggaaacggca gcaagtggta acgcaggatg aagtattcga cctgtatgaa
     8461 atcgacctgt cggacgagga cgccgccgct gaataaacag cggctttttt cattccaaca
     8521 acaaaatcaa aaaatcatta tgagccggaa accggcagaa agaggtaaat ttatggcatt
     8581 ttttaattcc gcagttacta ctttgcagac tatcgtgatc ggactgggcg gcgctctgtg
     8641 cgtatggggc ggcatcaatc tgttggaggg atacgggcag gacaatccgg gggcaaaatc
     8701 gcagggtgtc aagcagcttg tcgctggcgg cggcgtggcc ctgatcggtg tcactctggt
     8761 tcccctgctg tccgggctgc tgggctgatc ctggcctttg tccctgtcca aacccttaac
     8821 taatcgcctc cgcgtcctcc cggccagacc gggagggcgc gggaaaggag gtgctacccg
     8881 tgattagtga aatcattgaa gaatggataa aaggcatcct catggacggt atcacgggca
     8941 acctgtccgg gctgttcgat acggtaaatg ccaaagtcgg agaaatcgcc gccgacgtgg
     9001 gacgaacccc gcaggcatgg aacagcggta ttttcaatat gctgcacagc ctgtcggaaa
     9061 ccgtgatcgt ccccatagcc ggggccatac tcgcccttgt catgtgctat gagcttatcc
     9121 agatgataac ggaaaagaac aatatgcacg actttgacag ctccatgttc ttccgctgga
     9181 tattcaaatc cgcgtttgca atcctgatcg taacgaacac atggaacatc gtcatgggcg
     9241 tattcgacgc cacccaaagc gtcgtcaatc aaagcgccgg tgtgattatc gggcagacat
     9301 ccattgattt taacaccctg ataccagacc ttgaaagcca attggaggct atgagcgtgg
     9361 gcgctctttt ggggctgtgg ttccaaaccc ttgttgtggg cctcaccatg aatatccttt
     9421 ccatctgcat tttccttgtg acctatggcc gcatggtgga aatctatgtt gtcacgtcgc
     9481 tggggcctat cccccttgcc acaatgggca acgcggaatg gcgcggcatg ggccaagggt
     9541 atctgaaatc cctgctggcg ctgggcttcc aagccttttt aatcatggtt gtggtgggta
     9601 tctacgcggt gctgatacag aatatcggtt ccgcttccga catatccggc gcaatctggg
     9661 gctgtatggg ctatacggtg ctgctgtgct tctgcctgtt caagaccggc agtatatcaa
     9721 aggccgtatt cggggcgcat tgatcccaaa atccaagcat acacaggagg aacttgttta
     9781 tttcaacgta caacatcatt tacgctgacc cgccgtggcg gtatgactgc aaaaacgtac
     9841 agggcgcagc ggaaaatcac tattccacca tgagcattga cgagctgtgc gccctgccgg
     9901 tggaacggct ggcgtccaaa gactgcctgc tgtttttgtg ggccacattc cccatgctgc
     9961 cggaagctct gcggttaatc aaagcgtggg gcttttcatt caagaccgtg gcttttgtat
    10021 ggctgaaaca gaaccgtaaa agccttacat ggttttacgg gctgggccgc tggacaaggg
    10081 gaaacgcgga aatctgcctg ctggcaacac acggcaagcc gaagcgtcgt tctgcctctg
    10141 tccatcagtt tattatcagc ccgattgaac aacacagcaa aaagccggat gtgacccgtg
    10201 aaaaaatcgt agagctggcg ggcgatttgc cccgcgtgga gctgtttgcc agacagaaaa
    10261 cccccggctg ggacgtgtgg gggaacgagg tggaatgtga ccttatcctg acgtgaaagg
    10321 aggtgccaaa tggcttatgt aaccgtcccc aaggacttaa cccatgtgaa aagcaaggtt
    10381 gtgtttgggc ttaccaaacg gcaactgatc tgtttttccg gggcgctggt gatcggcgtc
    10441 cccttatatt ttctcacccg tgactatctt accaacagcg cggcggccct gctcatggta
    10501 tttgccatgc tgccgggctt tttgcttgcc atgtacgagc gtcacggcca gccccttgaa
    10561 gtggtgatta tgcagatcat ccagtgctgc ttcatccagc caaaggagcg tccctatcag
    10621 accgacaaca cctacaccgc ccttgtgcgg caataccaga tggaacagga ggtaaaggcc
    10681 attgtccaaa aaggaaatcc caagagaaac cgaaaaaaca aagctgaccc gcgcccagcg
    10741 aaaagaaatt gacgcggtaa tccgtaaata taagggcgac ggcaggcccc acacggcgca
    10801 gcagagcatc ccctacgagg tcatgtaccc ggacggcgtg tgccgggtat cgcccggcgt
    10861 cttttccaag tgcattgagt ttgcggacat cagctatcag cttgcccagc cggacacgca
    10921 gacggctatc tttgaaaaac tgtgcgacct gtataactat gtggacgcct ccattcatat
    10981 ccagttttct ttcctcaatc ggaaagttga cccggtgcag tacgccaaga gctttgagat
    11041 cgcgccgcag ggggacgact tcgacgacat ccgggccgag tacaccggca ttctgcaaaa
    11101 gcagcttgca aacggcaaca acggcatggt aaagaccaaa tacctgacat tcaccatcga
    11161 ggccgaaagc gtgaaagcgg cgcgagccag attgaagcgc atcggctttg acctgttagg
    11221 ctatttcaag agcatggggg ccgtagccca tgtgatggac ggctgggaac ggctgaacct
    11281 gctgcatggc gtgtaccacc cggacggcga gatattcaat tttgactgga agtggctggc
    11341 cccgtccggc ctctccacca aggattttat tgccccgtcc tccctgtgct tcggcaatgc
    11401 aaagacattc ggcatgggcg gcaaatacgg cgcggtaagt ttcctgcaaa tcctgtcccc
    11461 ggagctgtcg gacgatatgc tggccgactt cctcaatacg gaaagcggtg ttttagtcaa
    11521 tctccatgtg caggccattg aacagaccaa ggcgatcaag accatcaagc gcaaaattac
    11581 cgaccttgac gctatgaaga ttgcggagca gaaaaaggcc gtccgcagcg gttacgatat
    11641 ggacattctt cccagcgacc ttgccaccta cggcgaggac gccaagaagc tgctgacaaa
    11701 gttgcagacc cgcaatgaac ggctgttcca gttgacgttc cttgtcctca acgtggccga
    11761 caccaagcaa aagctgaata acgacgtgtt ccaagccgca ggcgtggccc aaaagcataa
    11821 ctgtcccctt gtccggctgg actaccagca ggaacagggc cttgcaagca gcctgccgct
    11881 gggcgtcaat cagatcaaga tacagcgcag ccttacgact tccagcgtgg ccgtgttcgt
    11941 cccctttgtg acgcaggagc tttttcaggg cggcgcggcc atgtattacg gcatcaacgc
    12001 caagtcccgc aacatgatta tgctggacag gaagcaggcc cgttgcccca atgccttgaa
    12061 gctcggcacc cccggcagcg gcaaatccat gagctgtaaa tccgagatcg tcagtgtgtt
    12121 cttaacgacg cctgacgata tttttatttc agacccggag gccgaatatt acccccttgt
    12181 caaacggctc cacggacagg taatccggct gtcgcctacc agcaaggact ttgtgaaccc
    12241 gctggacatc aacctgaatt attcggagga cgataacccg ctggccctga aatccgactt
    12301 tgtgctgtcg ttctgtgagc ttgtcatggg cggcaagaac gggctggaag ccattgaaaa
    12361 gaccgtgata gaccgggctg tgcgcgtgat ctaccggccc tatctggcag acccccgccc
    12421 ggagaatatg ccgatcctgt ccgacctcca caaagcgttg ttagatcagc atgtcccgga
    12481 ggctgaccgg gtggcgcagg cccttgacct gtatgtgtcc ggctccctga acgtatttaa
    12541 ccaccgtacc aacgtggaca tcggcaaccg gcttgtgtcc tttgacatta aggagctggg
    12601 caagcagctc aaaaagctgg ggatgctcat tgtccaagac cagatatggg ggcgcgtcac
    12661 ggccaaccga agccaaggaa aggccacatg gtattttgcc gacgagttcc atttgctttt
    12721 gaaagaggaa cagacggcgg cctattctgc ggagatttgg aagcggttta ggaaatgggg
    12781 cggcattcca acaggtgcca cccagaacgt caaagacctg ctttcctccc cggaaattga
    12841 aaatatcctt gaaaacagcg acttcattac gctgctcaat caggcgtccg gggatagaaa
    12901 aatccttgcg gaacgcttga atttgtccac cgaacagcag aagtacatcg acaattccga
    12961 acccggcgag ggccttttga tctttgaaaa cgtggtgctg ccctttacca accccatccc
    13021 gcacaacacc cagctttaca agattatgac gacccgctta aacgaggtgg cgggagtatg
    13081 acccggcgac gccacaaagg aggtgaacca ccatgaaaga gctgaaagcc aaggcgaaag
    13141 tgacgcaggc catgacccgc gacgggctga cgctggaaaa tcaggccacc ggcgaggtgg
    13201 agaacatttc cagccgggag gccgaacagg atttgtccgc gccggggccg ggcggcaccg
    13261 ccgataagct gctggaacgg gccgacgcca tccatgacgg ccataaggcc aagaaagccg
    13321 cgaagcaggc cggggctgtg gccgccgagg gggacgctgc cctgcaccgg ccatccgccc
    13381 ggctccaact gtcggaggaa gaacgggccg acccggattt acagccctat atccgaaaat
    13441 cggacaaacg ggccgataag ctggataagg cccgcgccgc catccccaag aagcgcgtcc
    13501 cggtcaagga aaccgtcttt gacgaggcaa gcggcaaagc aaagaccgtc ctgcactttg
    13561 acaaacagga caaggggcct ccctccctga aacccaatcc ggcaggccgt cctgtttccg
    13621 aggtttttct attcggccac gggaaaatcc atgaagtcga gcatgaaaac gtgggtgtgg
    13681 agggcggcca taagggggag gaacttgccg agcggcaggc cggaaaagcc atccgcagca
    13741 ccatccggca ccacaggctg aaaccctact gggacgcgga aaaggccgag cgcaggctat
    13801 tgtctgccaa cgcggagtat ttctaccaga aatctttgcg ggataacccg gagatcgcac
    13861 aggcggccag caaccccatt tcccggttct ggcagaaaca gcagatcaag cgccagtacg
    13921 ccaaggccgc ccggacagcg ggccagaccg caggcgcggc ggcacagggc gcggccaaga
    13981 cagccggggc ctccacgaaa gcggccaagg ccgcagccaa agaaagtgaa cgggcggcct
    14041 cctttgtggc ccgccactgg aaaggggcgc tggccgtcct tgtgttgggg cttttgatgc
    14101 tgcttatcat cggcggctta cagtcctgca ccgccatgtt cggcagcgcc ggaagcggca
    14161 tcacggcgtc ctcttacttt tccgaggata gcgatatgtt ggccgccgag gacgcctacg
    14221 cggagctgga agccgagctg caagactatc tggacaacta cgaggccacc catgactatg
    14281 acgaatacca ttttgacctt gacgagatca gccatgaccc ctatgtgctg atttccattc
    14341 tgtcggcgct ccatgacggg atatttacca tcggggaggt acagggtgat ttgtccatgc
    14401 tctttgacaa gcaatatatc ctgacagaaa acgtcgtggt agaaacccgt taccgcaccg
    14461 aaaccgatac atggacggac gcggagggca acacccatac agacacctac caagtgccgt
    14521 atgactatta catctgctac gtcacgctgg aaaacttcga cctgtcccat gtgccagtct
    14581 atatcatgga cgaggaaacg ctaagtttct attccctgta tatgtccacg ctaggaaacc
    14641 ggcctgatct gttttccggc agccagtacc ccaacgcctc caccctgacg ccgccgacct
    14701 actacgacgt acccccggag gctttggagg atgccacctt tgccgccatg ctcaaagagg
    14761 cggaaaaata tatcggttat ccctatgtgt ggggcggcag ctctcccagc accagctttg
    14821 attgcagcgg cttcatttcg tgggtaatca atcatagtgg atggaacgtg ggacggctgg
    14881 gggcgcaggg cctatataac atctgcaccc ctgtttccag ttcgcaggcc aagcccggcg
    14941 accttgtatt tttcaaggga acctacgaca cccccggcgt gtcccacgtc ggcctgtatg
    15001 tgggcaacaa tgtgatgctg cattgcggga acccgatttc ctacacgaac cttacttcaa
    15061 actactggca atcccatttt tacagttacg ggcgtttacc ttaacgccag aaaggagctt
    15121 tcaattatgg caaccaaact tgaccgcatc gaaaaggaca tccagaaaac caagtctaag
    15181 atcgcggagt ttcagaaaca gcttcgggag ctggaaacgc agaaaaccga acaggagaat
    15241 ttgcagatca tccagcttgt gcgcggcatg aacatgaagc cggaggaatt tgccgcgttc
    15301 ttacgcagcg gcgcaatgca ggccgcccct gccgcaaccc cttaccatga acaggaggac
    15361 aatgcccatg aagaataaga ccagacggat ttcccgtatt ctccccgcac ttctggccgt
    15421 gatgctctgc gtcacggcct ttcccgttac cgcccttgca ggcggcaatg acccggcacc
    15481 ggccccgctg ccggaggcta caaccgaacc cgctacgggc ggtttggagc ctgaaacaga
    15541 cgaaaccggc accgccacgg acgggacaga aaccgaggcc gaaaaagata ccggccctct
    15601 gaccggcaag gacatcaccg acctgttttc cgcgctgttc ggttccaagg tgagcattgc
    15661 cgccacggac gacggcatcc agatcaccac cggcaccgac aaaaaagaac ctgaacagac
    15721 cggcaccgtc accaccaacg gcggcaggct gaatgtccgc accggcgcgg ggcttgacaa
    15781 gaccgcgttc acccagcttc ccaatgggac gacggttgag gtagtcggca cagacggcga
    15841 ctggattaaa attctcttgc cggagcgcat cggctatgtc cattccgact atatgaccgt
    15901 cagcgaaaag gaagtagcgg ctaccggcga gggcggtttt tccctctcca ttgacccgga
    15961 ggaaattgct tctctgctgg aactgttcaa tggcggcggg gccagcgccg ccctgtcccc
    16021 ggacgggaac ctgtcactga ttgacgacat cggcagcgcc gcgaaaaatg gcaagcagtt
    16081 tatcaccgtg gaaaccaaaa acggcaatgt gttttatctg attattgacc gggacgacga
    16141 gggaaaagaa actgtccatt tcttaaatca ggtggatgaa gccgatctgc tgacgctgat
    16201 ggaggacggg gaaaccgtca cccccgctgc tgtctgctcc tgtaccacga aatgcaaggc
    16261 cggggctgtg aacacgaatt gcccggtatg taaaaccaac ctgaccgagt gcagcggccc
    16321 ggagccgcag gaaccccagc cggaggaacc cgaagccccg gaggaagaac ccaagggcgg
    16381 cgcgggcggc ctgatcgtgt tcctgctggt ggcgctggcg ggcggcggtg cggcccttta
    16441 ctatttcaag ttcaaaaagc ccaaggctga aaccaccggc agcgacgatt tgggcgagta
    16501 tgattttggg gaggacgagg atttggagga cgaggaaccc gaaaccgagt ttgacccgga
    16561 agctgatttt gtgagtgaac aggaggacga ggaatgacat ataaacttgt aatcgcggaa
    16621 aaacccagcg tggcccgcag cattgcgggc gtgatcgggg ccacggagaa acacgacggc
    16681 tatttggagg ggaacggcta tctggtgagc tggtgcatcg gacatctggt gtcctttgcc
    16741 gacgccggac gctatgacga gcgtttcaaa aaatggcgct atgaggattt gcccattctc
    16801 ccggaaacat ggcagtacat cgtccccgac gacaagaaac agcagtttga cgttctctgt
    16861 tcgctgatgc agcgccccga cgtgaccggc cttgtgtgcg ccaccgacgc cgggcgcgag
    16921 ggggaattga ttttccgttt cgtctaccag atggcgggat gccaaaagcc ctttgagcgc
    16981 ctttggattt cctctatgga ggacagtgct attaaggacg gctttgccca cctgaaaccg
    17041 ggcggcgact atgatccgct gtatcaatcg gccctgtgcc gggccaaggc cgactggctg
    17101 atcggcatta acgccacccg gcttttttcc gtcctgtacc acaagaacct gactgtgggc
    17161 cgcgtccaga cgccgacctt aaaaatgctg gtggacaggg acgccaagat taccgatttc
    17221 aagaaagaga aatatcacat cgtccatatc acggcgggcg gcgcggacgc ggtaagcagc
    17281 cgcttttccg acgctgccga ggcaaacacc gtcaaggcgg cttgtgcaaa agcgcaggcc
    17341 gtttgcgttt ccgtgacgcg ggagaaaaag acggaacagc cgcccaagct ctatgacctt
    17401 accaccttgc agcgggaggc taaccggctt ttcggctata ccgccaagca gacccttgac
    17461 tatgcccagg cactgtatct aaagaagctg ctgacctatc cgagaacgga tagtcaatat
    17521 cttaccgacg atatgctgcc cgccgtggaa agccttgtgt ccggcctgtg ggagctggtg
    17581 ccgtttgcca agggcctgaa cctttccccg cagtttgacc gcatcctgaa cagcaaaaag
    17641 gtgtccgacc accacgccat tatccccacc gctgaatttg tgaagcaggg ctttggcact
    17701 ctggccgaga gtgaaaaaaa gctgctgtcc ctgatctgct gcaagctctt atgtgccgtg
    17761 gccgggccgc atatctatga ggccgtcacc gccacattta cctgtgccgg gcaggagttt
    17821 accgccaagg gcaagaccgt cctttccccc ggctggaaag agattgaacg ccggttccgt
    17881 tcttccttaa agaccgacgc cgacgaggac gccgaggcgg tgcgggagct gccggagctt
    17941 tgcgagggcc agacctttgc agacgtggcc gcctctgtca ccgagcattt caccacgccg
    18001 cccaagccct acaccgaaga tacgcttttg tccgctatgg aacgggccgg ggccgaggat
    18061 atgccggagg atgccgagcg caagggatta ggcacaccgg ccacccgcgc cgccattctg
    18121 gaaaagctgg tgcagatggg ctttgtccag agaaaaggca agcagctcat tcccaccaag
    18181 gacggcatca atctggctgt ggtgctgccg gaggcgctta cttctcccca gcttactgcc
    18241 gaatgggaaa accggctgac cgagatcgcc aaggggcagg ccgatcctga cgaatttatg
    18301 gacggcatcg agacgcaggc gcgggagctg gtgcagacct attcctgtat cagtgaggaa
    18361 aagcagaaat tgtttcaggc cgagcgtgtt cctatcggga cgtgtccccg ctgtggtgag
    18421 gctgtctatg agggcaagaa aaactactac tgcggaaacc gggcctgcca gttcgtcatg
    18481 tggaagaatg accgattttt tgaggaacgc aagaaagcgt tcaccccgaa gatcgccgct
    18541 gcgctgctca aaaccggcaa agtcaaggta aagggcctct attccgtaaa gaccggcaaa
    18601 acctacgacg ggacggtgct tttggctgat accgggggca agtacgtcaa ctaccgcatt
    18661 gaacacagga gctaaaggac agtaacacag caagcatagg gagtgtggct acactcccgg
    18721 taaaatcccc cggccgcgtc cctgccgggc cggggatttt tgcttgttgg ggaagtcccc
    18781 aaccccctat tttttgaaac agaacagaga aaggagtgac cgcgtgaacg aagtattttc
    18841 tatccaagtc cataaccggc agtttttgga acagggcaaa caaggcgtct ggctggattt
    18901 gcccaccaca accgaaacgc tgcaagccgc cctgcgggat gttggcatta ctgccgacaa
    18961 tccgcaggat ttttttatca acgggtattc ctgcccggag gacagacacc ttgccattcc
    19021 ctatgacatg gtgctggccg ccgacgtgga cgagctgaat ttccttgccg cccggctggg
    19081 ccagcttgac gccgccgaga tcgcagagct gaacgccgcc ctgcaaaatc cccgtggcgg
    19141 ctttgagaat atcggccaga tcatcgacta caccgaaaat gtggattact atgtgcatct
    19201 tcccgacgtt aggggggatg gacagttggg cgactactac ctgaaccgtt ccggcatggt
    19261 ggatatgccg gaggaatgga aagccgggat tgatacggcg cagtttggac gccacattgc
    19321 ccagcaggaa caaggcgttt ttacccagta cggctatctg gtgagaagcg gcgacgaatg
    19381 gcagcgcgtc catgagggcc agccggtgcc ggaggaatac cgggtgatgc actaccccca
    19441 gccggaagct gaccgggagg aaccgcaggc ccagcagccg gaggcatccg aaagccccga
    19501 caaagcgccg cccccggtta tcccgatcat gctggacggc atgaacagca aagaacgcat
    19561 gaaagtgatt actgaccgtc tggaaaccgg cgtacaggag ctttttgaaa gtagccgcta
    19621 tatggcttac ttatccgcta tgtccaagat gcacgattac agtctgaaca atacgctgct
    19681 gatcgccatg cagggcggtt cgattgtggc gggctataac aggtggcagg atgaatttga
    19741 ccgccatgtg aagcggggcg agaaaggcat taagatactg gccccggccc cgttcaaggt
    19801 taagcagcag atggagaaaa ccgacccggc cacccagcag cccgtgatcg gcgcggacgg
    19861 caagcccgtg accgaagaac aggaagtgac ggttccggcc tttaaggtgg tgacagtctt
    19921 tgacatcagc cagaccgagg gcaaggattt tcccatgccc tatatcagtg aacttaccgg
    19981 cgacgtgcag cagtatcagg atttctttgc cgcgctggaa cggacatcac cctatcccat
    20041 aagcattgag ccgatttcag acggggccaa gggccgctgc ttctacgcaa aacagcgcat
    20101 tgccatcaac gagggcatga gcgaattgca gaccctaaag accgccatcc atgagattgc
    20161 ccatgccaag ctccacgcca ttgaccggga ggcccccgcc gaggaacagg cggcccggcc
    20221 agaccgcagc acccgcgagg tacaggccga aagcgtggcc tataccgtct gccagtatta
    20281 cgggctggac acgtcggact attcttttgg ctatgtggcc gattggagca gcggcaggga
    20341 gttatccgag ctgaaagcct cccttgaaac catccgggcc acggccagcg aattgataac
    20401 cgagatcgac ggccattttg cccagcttca gcaggagcgc gaggcccggc aggaacagga
    20461 ggccgtcacg gttgacgtta ccccggaggc agcggccccg gcgcaggccg aggaagaaca
    20521 ggccccggcc cttgatccgg cagccgagcc gcttgtgact gtgctatgga gcgaaagccc
    20581 ccagctaaag gacggccagc aaatgccgtt gcatgaggcc gacgcgctgt ttgcccgtct
    20641 ggatgcagca tatccggcag aggcaggcta tgacaaaacc gcgttccgca ttgactgtat
    20701 ctttcagggc gagccggaca gctacgaggg gcggcaggat tttggagata gggacggttc
    20761 cctgatcgaa catatccaga gctaccacca attttacgcg caggacgaaa actggaaaaa
    20821 gcatgtaatc gctacaaccg gcatagaggg ctgggagcag gacaaggccc atcgggatat
    20881 gctgttaggc gagtttgtcc cctatatgaa gctgcattgc agccttgccc aaatggaaca
    20941 gacggcgcag ggccgcttac agtccgggga aaccctgacg ccggaggaaa cggcctattt
    21001 gggggcgctt ctctcttatg tgcaggagtg ccgccccatg ctcaatcagg gccagtatca
    21061 gttgccggag ccgccgaaac tggctgactt cgaccagagc ttgcaggact acaaagcaca
    21121 ggtacaggcc gagatcgcgc aggaggccgc cgcagccggt atgaccgtgg aggaatacgc
    21181 ggcggctggc tatgaagcac cccaacagga caccttttcc atctaccagc ttcgaggcgg
    21241 ggaggaaacc cgcgactatc ggtttgagcc atacgagcgt ttacaggccg cagggctggc
    21301 cgtggacagt gccaactatg accttgttta tacggcaccg ctggataaaa gcacgtcttt
    21361 ggaggatatt taccgcacat tcaacatcga ccacccggca gattttaggg gacacagcct
    21421 ttccgtttcc gacgtggtgg tgctgcacca gaatggacag gacaccgccc actatgtaga
    21481 cagcttcggc tatatggaag tgccggagtt tttgcgggaa aatcccctgc gtaccgccga
    21541 actgtctacg gagcagaacg aaaacatgat tgacggcatt atcaacaata cgccgcccga
    21601 accgcagccg gaggaacccg aacagctatc ttttgaggat gtggcagttg cttccggcca
    21661 gacacaggag gaacccccgg aggctgcgcc cccgcaaacg gctgtccgct attatcccat
    21721 caatgaggga gccgcccgcc gcgccaagga agccatgagc ttttctgact accggcccgg
    21781 cagcgccacg gcggaatacc ggcactatgt cgatcaggcc gtggaaattg cggagcgcca
    21841 gaaaaagcgt gtggagccag agtatcacga aaagatagac agcctccttg acacctacgc
    21901 ccgcaagctg gccgagaaca tgaataaggg ctatgagatt accgcccgcg tcccctctgt
    21961 cctgattgcc gggccgtcca atttcccggt acgggcgaaa gaaaagcaga acgccgccag
    22021 tgaccgcaac atggaggaat ttcagtacat tcagggcttg cttgacaaaa tccgcagcac
    22081 cggcatgggt ggtatcagcg ccgacgatcc gcaggccgtt tccaagctgg aaaagaaact
    22141 ggaaaagctg gaagcgtcgc aggagttgat gaaggccgtc aatgcctact accgcaagca
    22201 tgggacgctg gacggatgcc cccatctgat cgaaagaggc attgaaaacc tgaaagcgga
    22261 tatggcgtcg ggctggcact atgagaaaaa accgttccag tcctggcagc tctccaacaa
    22321 taacgccgag atacgccggt taaagggccg cattgaggaa ttgacccgcc aaaaggaggc
    22381 ggcctatgtg ggatgggagt ttgacggcgg cactgtggag atcaaccggg tggccaaccg
    22441 cctgcaaatc ttctttgagg ggaagccgga tgctgctgta cgcgacgagc tgaaaagcaa
    22501 cggtttccgc tggtcgccca aggccggggc atggcagcgg cagttaaacg acaccacaat
    22561 ccgcgtcgct gaccgtatca agtgcatcca gcccctttcc ggcgaaaagc ccagcgcctt
    22621 acagcttgcg gcccgcaggg agaaagaaaa gccctctatc cgggcgcagc tcaacgcggc
    22681 caagaccgaa caggccaagc agccaccggc gcgggaaaaa tcaaagggat tggaggtaga
    22741 gtaaatgcag aagtttgaaa atgtggacgt tctggccgcg ctggaacaga tcatgcggca
    22801 gaacaccgcg ttttatcaaa gtgactttgg cattgacaaa gacattatca ggcgggatgc
    22861 cgccagcgat caggccgcag acaagaccct gctctggatg tcccggccct ccgggacgta
    22921 ctgtttccgg gaacgtgatg ttttcctgaa agacacccga cagtacaaca catggaaatt
    22981 ttacggggag cagacccgtg acaagatact ggcctacgcg gtggagctga ccggcacaca
    23041 gggcggcact atccggggca atctctatga gctggactat ccgcagcatt tccggcacgt
    23101 cattgaaacc gcccagccgg tgagtgtgaa ccggctcttt tatgagcatg gcacccgtga
    23161 catcccggag agccagcgtt ttgacggaag ccctgaccgg gtattgggaa actttttgta
    23221 ctatgaggcc cagccccatg acccggctgt gctgcaggag gcgctacgga cggagcagca
    23281 cagccggggg cgggccgtcc ccggcgattt caaagtccac gtcaccgctt tgcatgacag
    23341 ccggattgaa accgaggccc ggcgtattgt ggacagcctg aaagatctgt ccgcgcctaa
    23401 cagccccaat aaaacgcatt tcatggtgga gatctccccg cactttgcgg cgattgcttc
    23461 caccaaggat aatgaccggc tctttgccat gctgccctat aaatccctgt gttttaccgg
    23521 gatgaaaggc aggcatggcg tgtatgccgt gattaaccgg gacgagcgcc gggatgtgag
    23581 tattcgcagg ccccggccct ctatccgcaa gcagctatcc gacagcaaac aggccgccag
    23641 cccaaaaaag gcggcggcca gaaccaaaaa gaatgaattg gaggtataag gaatgatgca
    23701 ctttaccgtg gaggaagaaa acctgatctg tatgtaccac aatgcagacc ggcgcagaac
    23761 gataaacagg ctcaccgccg tcatgccggt tatggatggg gatatgcggg cgcaggccca
    23821 gcagaccctt tccaagctgg aacgcatgac ggatgccgac tatgacggcc agaagttcca
    23881 cttcacagac gaataacgaa cagcagacgc cgggcgcggc ggccatgtgg tcgcttgcgt
    23941 ccggcgtctt ttctttttgc ctattcctac gcatttagaa cccggtattg tcggggatag
    24001 gatgaagtat tcgcttgccc ctgcaaacat gcgggagaag ccttgcagcg ggcggttttc
    24061 aatcccttaa atgcgtaggt tgcagctata attagacccc tgaaacagat atagccgcgc
    24121 agcgggtgta tctgtttcag gggtgtgcag gtgatagccg caaagcggcg taagagggtg
    24181 cagccctctt gccgtctatc tctgttccag cgggaagctc ggcaatgctt ccagcagttt
    24241 cagggaaatg cgctgccgta tatcttcgtc aatgactttc ttctttttgc cgtttgcctg
    24301 tctgatctcc accgtggaaa gctcgttgat gtagtcagca taatgggcca acactttttc
    24361 cgtggcccac ctctcaccgg caacggcggc tttgatcgtt tcatagtcca gcagcttttc
    24421 ttgtccgtcc atcctgatac ccccgtcaga ctgatttttt ctatgatacc atattcggaa
    24481 gcatggaacc agagaaaacg gcgcttttct gctggtaaat tctacgcatt tagaacagca
    24541 cttcggcggg gataggataa aacacccaca taccccgctg accatgccgg aaaagccttg
    24601 aaatataagg gcttttacag ctctaagtgc gtaggtttcc agtatctttt ccgctggcgc
    24661 tcggcctctt tgcggcggcg catccgtttg gagcaggccg cgcagtattt ggcccggtta
    24721 gacttcggcg tgaacgtggc cccgcactca cagcagcgtt tcacttcgtc ccggtctttg
    24781 gtgatctcgg catacagggc cgcatccagc gggaggacag cggccttaaa ccagcggcac
    24841 agaagcgaat aggaaatgct ctgtacgcaa atacattctt cgccgtcgtc cagcaacagg
    24901 cagttgccgt cacaatagtt gcagcactca tgcgtcagct tccgggcctt gcgatattga
    24961 gggtaattca ttcgtggaat actcatagct cttgcgtcca ttcctttccc ggcgtcatac
    25021 gcaaaatgcc gtccacattc tgcttgatga tgccgtattc ctgcatctgc tttttcgctt
    25081 cgccgtactt ctcatacagc cgttcctttt cctcggccaa tcgcttgtac tcggctttca
    25141 gggcgtacag atcggggagc tttttcacac cctgtccctt tagggccttt gccgcagctt
    25201 catacaaaat caacgtgctt tcatgctcac gccggaattt tgccttatcc gctgcattgc
    25261 ggtattccat agccacgggc cagagctgct tgtaggtaga aatattcttt atcagcaccg
    25321 ccatatccgc aagccggtgt tccatagctt tgagggtagc ggcggcctcg tcgctggcgg
    25381 cggtgatctc ggccactttg ctttccaaat ccgggtaggt gtctatccca tgttcagtca
    25441 ggaaattcaa agtctttgcg gcctgcttca aattgtggag cttcgcccac ctctcatagc
    25501 ctgccgactg ctgggcctta atgttgtttt ccagatcaat ccgcaaagaa atccccttgc
    25561 gctctttggg cgctctggtg ataacgcggc ctttgatacg ctctttgatg gcttcctcgg
    25621 tgtaggcttc ccccaatgtc ttgcagcggg taaagcgttc ctgtccggga gcgcggaaag
    25681 aaataaactt tcccggcttg atctcatagc cctgcgcctg catgagccgc aggaaatcat
    25741 caaaatcttt tgcctgcggg atagcggcgt caatggctgc tttgagcttc gctttccagc
    25801 tcgtcccttt ccgctgggcg tcccactcgg catagctctt gcccttactc tggccgggct
    25861 tcaccacgga caatccgttt tccttgcaca gcctgtcgct ggtgcgccgg atataggcat
    25921 agctctgttt gttggaaatg tacttgcgct gatttaccat atccacggca cagaaaataa
    25981 tgtgattgtg gacgtggccc ttatcaatat gggtagtcag cacataggga tatttccctt
    26041 gcagcacttc ctcggcaagc tgcttcccga tctcatgggc ctgttccggc gtggcttcgc
    26101 cgggggcaaa ggattgtatc aggtggtggc ccagattatt gcccttttgg tatgcctgcg
    26161 ccagcagcat ttcaaattca atgtccgctg tttcataaga gcaggcaaag gaggccacca
    26221 gcagcttgcc gtcagttttg tccgggttct cgatgtagtc aagggctttt ttcagcgttg
    26281 acttgatagg atgtatcttt gtaactgcca tatctgcgcc agcgccccct ttatatcctc
    26341 gatgtcctgt gcgtacaccg gccccatacc gttcagcctc tttgcaatct gattgacgtt
    26401 gacgccgatc ttctgtatct cggcggtctg cgcccggatg tcgctggtgt ccatgttgac
    26461 gatatagccg tcaatgagca tcttccgggc gtaggctgaa aagttcttcg tatgcagcag
    26521 cgccattttc tgttcaatca tagcccgttc ctccggcgtc acagggacgc gcagcacgat
    26581 ttttcgcttt ctgtttgcca tgcttcaccg tcctttccat tcaggggttt ggggatttcc
    26641 ccaacaagca aaacggacgc ggcccggcag ggacggggca ggccgttttt ccgggagtgg
    26701 ctacactccc tatgcttgct acgttactgt ttctccacta acaagacgtt tccaaagccc
    26761 caaactaccc gcaaaatagc cctctggaaa gaaaaaagtg caaaaaaata gaagctacgc
    26821 tgtgcagctc ccgctaaaag taaaacagag gatgccagtt acacatcctc cgtttcattc
    26881 ttcttcggct tttcaattag tatggcgctg tcaatcgcgc cctcgatgat cggcaggtat
    26941 tcttcggggc agagtttcag cttgtggctg accctttgcc gcagttcgct ttcctcccgc
    27001 atgatctccg ggttaaagta gcgttccaca ggaagcccgc aggctttaat aagctgtatg
    27061 acaaccggca gactgggaat ggcccgtcgt tttcgatgtt ggcaagatag cgccagtcaa
    27121 tatttatttt ctctgccatt gctttgcgcg aaacgcgctt tgcttgccgg gccgctttca
    27181 catccgcgcc gaaagtttca aagccgggac aatcttcaat attcgccata tcgcacctcc
    27241 cacttacatt gtatatttca tactctgccc gtggaatgtt ggtatatgca cctttcttaa
    27301 agtttatatt tcacattttg tttcttttat tcttcgagca aaagaattat aatgtaccct
    27361 gtggaggtgt actatggact acatgacgtt gaaagaggca ggcgagaaat ggggtgtgcc
    27421 cccacgatgg ataaactact attgtgctgc gggacgcatc cccggcgctg taaagatggc
    27481 gacgatctgg ctcattccca aaactgcgga aaagccgatt gacaggcgta caaagaaagg
    27541 aaaggcggtt actgaatagt ataatgaaac cggcgcattt tggatatggt tttgaagaga
    27601 gagaaagcat ggtattttgt cacggaaaaa ctataagatg atagatggca ggctgctcca
    27661 gaccgataaa aagtattctc atctgaaaca gaaagaaaag atttacacat ggatgtatga
    27721 agaagcgaaa ctttatcatg acagaaatgg aaaatgcccg aaacaaagac gaggaagctg
    27781 ctgctgtatc agccgtatat ggtagaattg aaaaggccgg catctggatt ctttatggtg
    27841 aagtctccag acattggata actgctggtc taatgcgcct ttgagtatgg ggtgattgat
    27901 tttaataccg gggaggtacg gctttttgag gaaaatattc tgggatttac atttggcgat
    27961 tattacattt cctgtaactg caaggacacg gatgatgaag atatgaggaa tatagacttg
    28021 ctgattttct actgtcttga acggtattag gactgaatct gctgcgcgga gtgcggcaag
    28081 agcgaccgga gggagtacac acaggccctg cctgtgtgaa aagccatacc gcaaggtgca
    28141 ttcgcaaaaa agaggccgcg cattttgctg aggcttgggg tgtccccaac aagcaaaacg
    28201 agaacgccgc aggcgggcaa gtggtggatc agaatatccg taaaggatat tctgatccat
    28261 tgcttgccag tattatcggg atgttttgac aaccgctgta atcgaaacat cccaaacatt
    28321 aagaaggtgt taattattat tatcttgatt taattcgttt aaaccaataa tatagataca
    28381 tacaccatga aaagaagcga gtatcccaat gacggatgat taaaatccga aaagaaatcg
    28441 ctttgaaact acagggaaac tacagactgt tatgttatct tcttaaaatg gagggatttt
    28501 tatgtcgata cgaattctac ttgtcgagga tgatgatcat atctgtaata cagtaagggc
    28561 gtttctggct gaggcaggat atcaggtgga tgcctgcaca gatggaaatg aggcatacac
    28621 caagttttac gaaaacactt atcaactggt tattcttgat attatgctgc ccggtatgaa
    28681 cgggcatgaa cttttgcgtg aatttcgtgc gaaaaatgat actcccattc tgatgatgac
    28741 agccctgtcg gatgacgaaa accaaatccg ggcgtttgat gcagaggcag acgactatgt
    28801 aacaaagcca ttcaagatgc agattttact aaagcgggtg gaagccctgc tgcggcgcag
    28861 cggtgcgctg gcaaaggaaa tccgtgtcgg caggctgaca cttctgccgg aggatttcac
    28921 ggtactttgt gacggtacag agctgcccct gacacgaaaa gaatttgaaa tccttttgct
    28981 gctggtgcag aacaaaggca gaaccttaac gcatgaaatc attttgtcac gtatatgggg
    29041 atatgacttt gagggtgatg gcagcacagt ccacactcat atcaaaaatt tgcgggccaa
    29101 gctgccggaa aatatcatca aaaccattcg cggtgtagga taccgattgg aggaatcata
    29161 ataatggaaa gaaaagggat tttcattaag gttttttcct atacgatcat tgtcctgcta
    29221 ctgcttgtcg gtgtaacagc aacactgttt gcacagcaat ttgtgtctta tttcagagtg
    29281 atggaattac agcaaacagt aaaatcctat cagccattgg tggaactgat tcagaatagc
    29341 gataggcttg atattcaaga ggtggcaggg ctgtttcact acaataacca atcctttgag
    29401 ttttatattg aagataaaga gggaagcgta ctctatgcca caccaaatgc caatacatca
    29461 aatagtttta gacccaactt tctttatgtg gtacatagag atgataatat ttcgattgtt
    29521 gctcaaagca aggcaggtgt gggattgctt tatcaagggc tgacaattcg gggaattgtt
    29581 atgattgcga taatggttgt attcagcctt ttatgcgcgt atatctttgc gcggcaaatg
    29641 acaacgccga tcaaagcctt agcggacagt gcgaataaaa tggcaaacct gaaagatgta
    29701 ccgccgccgc tggagcgaaa ggatgagctt ggcgcactgg ctcatgatat gcattccatg
    29761 tatgtcaggc tgaaagaaac catcgcaagg ctggaggatg aaatcgcaag ggaacatgag
    29821 ttggaggaaa cacagcgata tttctttgcg gcagcttctc atgagctaaa aacgcccatc
    29881 gcggctacaa gcgttctgtt ggagggaatg cttgaaaata tcggtgacta caaagatcat
    29941 tctaagtatc tgcgcgaatg catcaaaatg atggataggc agggcaaaat catttccgaa
    30001 atactggagc ttgtcagcct gaatgatggg agaatcgtac ccatagctga accgttggac
    30061 atagggcgca cggttgccga gttgctgccc gattttcaaa ccttggcaga ggcaaacaac
    30121 cagcggttcg tcacagatat tccagccggg caaattgtcc tgtccgatcc gaggctgctc
    30181 caaaaggcac tatccaatgt catattgaat gcagttcaga acacgccgca gggaggcgag
    30241 gtacggatat ggagtgagcc tggtgctgaa aaatgccgcc tttttgtttt gaacatgggc
    30301 gttcacattg atgatactgc gcttccaagg ctgttcaccc cattctatcg cattgatcag
    30361 gcgcgaagca gaaaaagtgg gcgaagcaga aaaagtgggc gaagcggttt aggacttgcc
    30421 atcgtacaaa aaacgctgga tgccatgagc ctccagtatg cgctggaaaa cacctcggat
    30481 ggcgttttgt tctggctgga tttaccgctc acatcaacat tgtaaatatt taaaacttaa
    30541 atgattttgg gctctttgtc aaataggact gatagagtga gataagaac
//
LOCUS       DADVXU010000291        16982 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.guided.060, whole genome shotgun sequence.
ACCESSION   DADVXU010000291 DADVXU010000000
VERSION     DADVXU010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.guided.060"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(134..577)
                     /locus_tag="KB953_003098"
     CDS             complement(134..577)
                     /locus_tag="KB953_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HBB6867976.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="KB953_003099"
     CDS             complement(795..2135)
                     /locus_tag="KB953_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBB6867977.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="KB953_003100"
     CDS             2404..3300
                     /locus_tag="KB953_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HBB6867978.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="KB953_003101"
     CDS             complement(3450..4391)
                     /locus_tag="KB953_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBB6867979.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="KB953_003102"
     CDS             4517..5638
                     /locus_tag="KB953_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBB6867980.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /gene="eat(A)"
                     /locus_tag="KB953_003103"
     CDS             5896..7398
                     /gene="eat(A)"
                     /locus_tag="KB953_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HBB6867981.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7426..7992
                     /locus_tag="KB953_003104"
     CDS             7426..7992
                     /locus_tag="KB953_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBB6867982.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            8203..9264
                     /locus_tag="KB953_003105"
     CDS             8203..9264
                     /locus_tag="KB953_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBB6867983.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9267..10163
                     /gene="murQ"
                     /locus_tag="KB953_003106"
     CDS             9267..10163
                     /gene="murQ"
                     /locus_tag="KB953_003106"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBB6867984.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            10177..12159
                     /locus_tag="KB953_003107"
     CDS             10177..12159
                     /locus_tag="KB953_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBB6867985.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="KB953_003108"
     CDS             complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="KB953_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBB6867986.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            12881..14305
                     /locus_tag="KB953_003109"
     CDS             12881..14305
                     /locus_tag="KB953_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBB6867987.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(14486..15259)
                     /locus_tag="KB953_003110"
     CDS             complement(14486..15259)
                     /locus_tag="KB953_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBB6867988.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(15256..16161)
                     /locus_tag="KB953_003111"
     CDS             complement(15256..16161)
                     /locus_tag="KB953_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBB6867989.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(16315..16635)
                     /locus_tag="KB953_003112"
     CDS             complement(16315..16635)
                     /locus_tag="KB953_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HBB6867990.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         5487 a         3098 c         3482 g         4915 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
     8161 tagtcataga aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata
     8221 tttatgaacg attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat
     8281 ggctttgtcg gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa
     8341 aagcgattga tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat
     8401 atttcaggag aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa
     8461 gcaattggtg taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac
     8521 tggtcacatc aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat
     8581 gaattgaaag aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca
     8641 cgtcccgaaa cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt
     8701 caaggtctcc ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat
     8761 caaggattgc caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat
     8821 ctattagcaa gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt
     8881 caggagcaat ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta
     8941 gatgaagaat tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga
     9001 gatgtcatac gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat
     9061 acagctacgc gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg
     9121 ggtgagatcc aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct
     9181 aaagttatca aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa
     9241 tttataagaa aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg
     9301 aacgcagaaa ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac
     9361 taatgaacaa agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg
     9421 aaccagtgat tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg
     9481 gagccggaac aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg
     9541 gtgtagaacc ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag
     9601 ctgtcgaagg ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt
     9661 tgacaaaaaa tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg
     9721 gtggattaga ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac
     9781 aagccgaaat cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt
     9841 tgacaggctc tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt
     9901 ctactatcag tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac
     9961 caacaaatga aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg
    10021 attacgaaac agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg
    10081 tcatgatttt gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg
    10141 gatttgtaaa agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg
    10201 gaagaactgg caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt
    10261 gtccactgca tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct
    10321 gaattaaaag cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc
    10381 atcggacctg ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa
    10441 ttaggagaaa aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa
    10501 gaccgtgtaa acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca
    10561 tctaaaatca aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca
    10621 tttgtcggta ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc
    10681 ggaacacttg atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac
    10741 gggatgttta gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca
    10801 acaccaactc ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa
    10861 gcaccactga aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta
    10921 ggtgtcatct tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta
    10981 cctgatgcgg tagatatcat cgttacacca actctttcat tgattgccat cggtctgatt
    11041 gaaattttct tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc
    11101 aactgggtac ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg
    11161 ccgatggtta tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat
    11221 gaaactggtc gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt
    11281 gcagcattgg cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa
    11341 ggagctttac cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta
    11401 cctctaggac gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc
    11461 ggaatgatcg gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca
    11521 ttgatctctg acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt
    11581 ggcttcgttg ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta
    11641 gcgactgaaa cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct
    11701 gaaagtgtga ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca
    11761 agtgatccag tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac
    11821 ggacatatct attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct
    11881 attgggatca caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt
    11941 gacttaggcg gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat
    12001 acgcttgtag cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg
    12061 atcgtcttgg taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa
    12121 gcaaaagcaa aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac
    12181 gaaagaagaa actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg
    12241 ctaaaatctg ataaattcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca
    12301 agaaaggaaa gccaaatgtt tgtgcatatt gtttggctgt tagacaatac tgattattga
    12361 agtttttatt tccgctaccg actacaccaa gacaaagttt atgattgtct tcatgttcca
    12421 gatattcacg catcgtttcg gtcaagattt ctgtatcacc gttatctaaa ccatttccgc
    12481 cgtctaaata agtaggaacg aaagtaaaaa atggttctgt ttctttcgta aaatcactat
    12541 tttcatggat ttcttttaaa gtaacttcag gattgacagg atcttctgca tgcatttttt
    12601 cagcatattc tgctagatgt ttggcaaagg cacgcgtatt tcctgaaatc gatatatata
    12661 aaatgttcat ggatcatcct ccaattcttt ccttttagta tagcatcttt tttaagtttt
    12721 ttggtggtgt aatttggaaa gggactaggc gctttgcttg tataatgaga aaaaagccgg
    12781 tagaattata tttctattaa agtttttatt aattaagtaa aaataaaaaa ggtttgttat
    12841 actttttgat aaaagcggtt ttattttgga ggatgcttac atggatacag agcaacagaa
    12901 aaaaggaatt ggtttaattc cacttgctgc cttagtaatt ggttcagcga ttggaggtgg
    12961 cgtatttggt attatgacgg acatctccgg atcggctggc ggcccagcta ttttcagttg
    13021 ggtactcgta ggaacagcaa tgttgatgct ctccttgtcg atcaataata tcaataaaaa
    13081 acgtccagat ttaaaaggcg ggatctttag ttacgcagaa gaaggttttg gccgtttcgc
    13141 tggttttatc agcggttggg gttactggct cacatgctgg ttaggcaacg tagcttttgc
    13201 aacattgttg atgagcgcat taggatattt cttcccagta tttgaaggtg ggcaaaatct
    13261 ggcatcgatc gtgatgagta cagtcttttt atggggatat gcatggctag tgaatcgggg
    13321 agtcgaaaat gctagtttcg tcaatgctat cgtcacattc tgtaaattag tgccattatt
    13381 tttatttatt gtagtagcta tcagcacgtt ttcgatccaa caattcattg ataacttttc
    13441 tagcgacatt atattgaatg cgaatggaca gccgatcccg ttttccagtc aagtattgag
    13501 ttgcatcatg gtgatgcttt gggtatttgt tggtattgaa ggagcctctg tcgtaggttc
    13561 acgagcaaaa aaacaatcag atgttgggaa agcaacgatt ctagggatat tcggcttgat
    13621 tattatttac gtcttggttt ccatgcttcc atatgggaca atgacgatga atcagctgca
    13681 aacgatcaag gcgcagcctg ctatgggcta tgtgtttgaa atgatggttg gtaaatgggg
    13741 agcagtcgtg atcaacggcg gtttgatcat ttctttagtt ggtgtttggc tctcatggac
    13801 gatcttaccg attgaaacaa tgggaaacat ggctgaagac ggacttttgc ctaaagcatg
    13861 gggaaaactc aacaaaaatg gggcaccaac ttatgctatc gtattgacga cgttatgtac
    13921 gaatgtcttt ttactttcat tattattcac tgacagtgct tataactttg cttacacact
    13981 tggtacagca gctattttct tcacgtggct tttcttagga ttgtaccaaa tgaaactttc
    14041 ttatcagcgg aaagaatggg tccaattttt agtagggctt ttagcaagtg cctttcaagt
    14101 atgggctatg ctagtcgtag cctttaatga agtaatgctt gttcttgttc ttttcttgcc
    14161 aggcatttat ttttacctca aagcacgtaa ggagcaaaac gagcctgtac aacatatcca
    14221 cttagataag attttactta taggtgtgac gatggtcgga gtgattgcaa tggttttatt
    14281 tgccactggg atgcttcacg tatagaagta taaaagaagt ataaagagag gttgtgagcc
    14341 tgtcgtttat tcaaaaacat actaggaaag gagctgtgac acaaatgttg tcacagctcc
    14401 tttttcagaa taaacggtgt ttaagagaca gccaatcggc aataaggctg aacgtctctt
    14461 tcacagcctc ttttttgatg agtcatcata cattttttcg cttaaatacg agatatccta
    14521 atgctaagaa caaaacgatg taagctaaat taccgaatag catttgatta gtagaaagat
    14581 gagtcaattg cttcatgatc tcttcgtttc caatttggtt ttgcagattc aacatactga
    14641 tcggattcca tttgatccac tcccattttt ggatgagtgc aataaggata ccagatatga
    14701 ttgacgaagc aaaataaaag acgattcctg ctgagatgga tgcgccggaa ctattgataa
    14761 agcaagcaat catcaacact aaacttaaaa tcagccataa accgatataa ctacccaaag
    14821 tataagtgaa caagctttgg atcagtgttt gaccagtaga aaccttctcc gtgaaggaaa
    14881 tcgaagggaa aagaatcagt ttcatcaaca cagtgacaat aatagctaaa aggtataaat
    14941 aaacagaata aataactaat gtgatccatt tgcttactaa tatttctcct cgagaatact
    15001 ttcgataaag gagatttttc aatgtaccat attgtgcttc catcgaaatg atcgtgcttg
    15061 cagctgcaat catgataaag acaatccaag atgtggcact aaaaagctga gcagtcattt
    15121 caactggatc aatgatcgtc gtgtattttt tggctagaag tgctgttcct atgagcaaaa
    15181 caactaatag aacagataaa acagcagttg actttttctt gatcaattta aataattctt
    15241 gttttacaag caatgtcatt tttcttcttc cccctttact gaatccagaa tttccaatag
    15301 agaagattcc aaatcattcg tcagatgctg gacgtctgtc acatggatat tttgtgcaga
    15361 caatagctgg atcacggttc ctaataattc ttcgttttct tttaaaagct tgattttatc
    15421 aggatcttcc aaagtatatc cagcttctgt caagatcgtt cgagcagcta gatcatttgt
    15481 tgtagagata acgatgaaat cttgcgctgt ttggttcaat tcagccattg tacattgttt
    15541 gatgatcttt ccttgattga tcaggatcag ctcatcgacc attttttcaa gctcgctgag
    15601 taagtgactg gagatcaaga aagaggtgcc ctgagtagcc aagtcggtaa tgatttttcg
    15661 cacatctctt gttgcttgtg gatccaaacc gttcatcggc tcatctaaaa taacgataga
    15721 cggatggttc agtaaggcaa gagcaattcc caatttttgt ttcattccca acgagtagga
    15781 tttcgctttt ttgtgaatgt acttttccat cttgagctga ttcacgatag tgtcgatagc
    15841 agatacatcg ttggtttcag agaataactt cagatgatca tagcctgtta agaatggata
    15901 gatacccggg tattcgatca atgctcctac ttgttccaac cctttatgac tagagggaga
    15961 aatctcattt tcaccaatac agattgttcc atctgagtaa tggatcagtc ctaaaatagc
    16021 tttcatgatc gttgtttttc cagcgccatt aggacctact aatccaacga ttttgccagg
    16081 ggataagcta aaactgacat cgctgagggc ttgtttgtga ccaaagttct tggataattc
    16141 ttggaccgaa agacttttca aaaatattca ctccttttct atagatagta caagcataca
    16201 cctgatgagt ggagaagtcg ctaggtcatt ggacttattt cttatttttt cataaaaaaa
    16261 ttgaatatta aataaaaagg cttatttttt ctgatcagta cttattccaa gtgtctagtg
    16321 gccgctagta atcttgatgc caatgatact gatgagtagc atccccacaa agaaccaagt
    16381 ggaaagattc agcttatcat gaaacaagac cacaccaatt atgaccgaac caactgcgcc
    16441 aatccctgtc cagataggat aagagagact catcggcaaa cttttgattg cctttgacaa
    16501 aaagtaaaaa ctagcaatca tgccaatcac tgtgtaaaaa gaataattga ttttagaaaa
    16561 accatcactc catttcatca tcgttgacca gaatacttcc aaaattcctg ctatgattaa
    16621 ttccatccat gccatactta cattcctcct taactataaa aaaatacaca aaaagaggaa
    16681 aatcatccct tcaaagacct aatggaatga tcatcctctt tatcctaccc ggtggcagat
    16741 tgtatatctt tttctgtagt atagcacacc tatccagata tcggcaatct catacttttt
    16801 agtaaagtgt accaagcact gacggatata tcgttagcgg aaaagaagat gaatgctttg
    16861 caatttcata gaaagaagtt gttatttatt aatcatttaa aaaaaacagt aactagacct
    16921 tatttctata tattatcggg caataaatta aaaaaagctt taccttacaa taagtaagat
    16981 aa
//
LOCUS       DADVXU010000292        13213 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.guided.079, whole genome shotgun sequence.
ACCESSION   DADVXU010000292 DADVXU010000000
VERSION     DADVXU010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.guided.079"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            complement(204..2162)
                     /locus_tag="KB953_003113"
     CDS             complement(204..2162)
                     /locus_tag="KB953_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBB6867991.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(2159..3031)
                     /locus_tag="KB953_003114"
     CDS             complement(2159..3031)
                     /locus_tag="KB953_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBB6867992.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(3373..4245)
                     /locus_tag="KB953_003115"
     CDS             complement(3373..4245)
                     /locus_tag="KB953_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBB6867993.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            4373..4942
                     /locus_tag="KB953_003116"
     CDS             4373..4942
                     /locus_tag="KB953_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBB6867994.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            5009..5746
                     /locus_tag="KB953_003117"
     CDS             5009..5746
                     /locus_tag="KB953_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBB6867995.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            5977..7245
                     /locus_tag="KB953_003118"
     CDS             5977..7245
                     /locus_tag="KB953_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBB6867996.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            7732..8196
                     /locus_tag="KB953_003119"
     CDS             7732..8196
                     /locus_tag="KB953_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBB6867997.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            8180..8809
                     /locus_tag="KB953_003120"
     CDS             8180..8809
                     /locus_tag="KB953_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBB6867998.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            9163..10641
                     /gene="msr(C)"
                     /locus_tag="KB953_003121"
     CDS             9163..10641
                     /gene="msr(C)"
                     /locus_tag="KB953_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HBB6867999.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            10688..11134
                     /locus_tag="KB953_003122"
     CDS             10688..11134
                     /locus_tag="KB953_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HBB6868000.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(11333..11848)
                     /locus_tag="KB953_003123"
     CDS             complement(11333..11848)
                     /locus_tag="KB953_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBB6868001.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            11965..13167
                     /locus_tag="KB953_003124"
     CDS             11965..13167
                     /locus_tag="KB953_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBB6868002.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         4116 a         2424 c         2708 g         3965 t
ORIGIN      
        1 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa gagtcaaata aaaaatagct
       61 cttcatatgt tacgtttggc tggacttatt accgcggagt tggcaggaga acgccgtttt
      121 tattcggaaa aaggagctga gacaagattt ttgtctcagc tcctgtttgt ttatcgataa
      181 aaaagtctgg ggaagacgat ggctcaggct ttgttggaaa cagagccgat tttatcattt
      241 gcttttacat cacgtgcggg ttcgatttct aagctttgga ctttgtcgcc atttgtgaat
      301 acaacgacca tatccgtgtt tttgcctgca gattgaatag cggcaagatc aactaaagca
      361 atcaattgac ctcttgcgac tttttgacct tcttttacat ataaagtgaa tggttcgcct
      421 tttaagtcaa cagtgttgat gcccatatga agcaagactt cgataccagc atctgtttgg
      481 attccgacag catgtttcgt tgggaaaatg tttgtgatcg taccagcgat tggagagaag
      541 atttctccat tttctggtaa tacagcatag ccgtcaccca tcattttttc cgcgaataca
      601 tcatctgaaa cttcagtgat tggaatcagt ttcccatttg cgatagcgta aatatcttgt
      661 tcgatcgttc caccggcaaa tgtttctgtt ttaaccacgg tgtttgtttg tgcaggtcgt
      721 gttgcagcac caccatgtag ttttgccatc tcatctgcaa cgaattgtac ttctgtacct
      781 acgatgactt gcgcattttt tccgtcgatg atcttcatac caggtacgcc agtggctttg
      841 attttatttt gatcgatcag gcctgtatct ttgacttgca aacgaaggcg tgtagtacag
      901 ttatcgatcg aagttacatt gtcggctccg cctaatgcag cgtagatttt gcttgcttgt
      961 gccgcatatt tattgtctga attagtcgtt gcagtatcgt catctgaaag atctgcttcc
     1021 tcgcggccag gagtcatcag atggaatttt ttgatagcaa agttaaagcc aaagtagtaa
     1081 atcactgctg ttactaatcc ttggacgatc aacatcaatg gctgattcgc aatcggattt
     1141 ttcaaagata ggaagtaatc gacaaatccg gcactaaagg caaatcctgc tgtccaatgg
     1201 aagaatgcac tcactgctaa agaaatccca gtgaaaattg catgcaagac atataatggc
     1261 caagcaacga acataaatga gaattcaagt ggttctgtta caccagtaaa gaatgaagca
     1321 aaagctgctg ctaacatcaa ggaagctgtt tttgctttgt tttctggacg tgcatttctg
     1381 taaatagcat atgctcctgc tggaagcccg aacatcatga ttgggaaaaa tccagcttga
     1441 tacattcctg ttacgccttt tgttccttca gaagccaaaa atttaccaat atcattgatt
     1501 cctgctacgt caaaccagaa cacggagttc attgcttgat gcaatcctgt agggatcagt
     1561 aaacggttga agaagccata aagacctgca ccaccccagc ctaaactcaa aatgaattta
     1621 gcaaaagaaa ctaaagcaga gtaaaccgga ggccatacga gcagtagcac tgcactgatc
     1681 agcaacatga caaaggcagc caagattggc actagacgct tgccgctaaa gaaagataaa
     1741 gccatcggta attttgcttc gtggaaacga ttataaagag ctgccgcaat caatccggca
     1801 ataataccaa taaatacgtt actgatctga gcaaaagatg gatcaactga ttctggtttg
     1861 atattcaata aagtagctac tgaatcagtt tttaaaacat ttactggaac ttcaaatgcg
     1921 accaaacctg ccaaggcagc agcacctgat ttgtccactg acatccctag tgctaaccca
     1981 acggcaaaca gcaaaggtaa ttgacctaaa actgcgttac cgccagcttg taaaaaatga
     2041 gcgacaatgt cgttactata agatgcccac cagtttccga tccctactag taaagcagca
     2101 gcaggtaaga ccgcaactgg aagttgaagg gaacgtccca ttcgttgtag atatgctttc
     2161 aatttttttc ctcctcatga ttgttaacat ttgtttgtac gattgcgctt cttaaacgct
     2221 cgatatggat ggccaagtaa cccagttctt ctttcgtgat cgcgagctgg tattctttgg
     2281 tcagcaacac acggattttt ttagaaatct tatagctttc tgggtatttt cgtttgacca
     2341 tttctacgat ttccgtgtct aaaacagcat attgttgcgc atgggttcgt tgtagaagca
     2401 gtcgtaaatg attcaccagt cttgaataat tcagtgaaaa tggttctgaa tggatatctg
     2461 tttccaattc tgattccact aaatggatga tgtcagatac aatcgtcact tcacggatac
     2521 tgcgatggtt attgttccga ccgcttcgtc cgctgtgaat atggattgcg atatatcctg
     2581 cttcgtcata agtatatgga atcccaagct cttttgtcag atactctact gcccattgag
     2641 caatactgaa ttcatcactg taaagcactt cgatttctct taaaagtttg ttgcggatga
     2701 cgataccgtt attaatgttc tccgcagaaa aagcaatgtg gtcggtcaaa gcgatcaata
     2761 catgttcatt caacttttcc atcaaaacag tttccgcatg atcaatgatc gtttctgctg
     2821 caaataaaaa gcgttcatca atctgattaa gtaaattttg caacttgatt tgaccttctg
     2881 gttccatgac aaatatccgt tcgacatcac gatcaaaaat caaatcattt ctttttctgt
     2941 caaatccaat cccttttcca atagctactt tttcctgtcc accgtcatct accaatactg
     3001 cattttgatt aaagatcttt ttgattttca tgcgcgcacc ctttttgaag atttggtttt
     3061 cacgtttgct agaccagttg attgcggcca aacaaaaata gacacgaaaa acctcctagg
     3121 gaaaccatgt tcctcagaga tttttcatgc ctagtatgat ctagtcacat gaaacgaatt
     3181 attgttaacg taatcaattt tatatagaca gttatgtttt gtcaattctt tttttataat
     3241 tatagtaatt tactggaatt atcagaaata aaaggaagga acaaattaag aaacttatgt
     3301 tatttaatca ttggatcttt tgcttaactt attcgttgtt aaaaagctga aagcgaagga
     3361 aaaaaactct acttaactgt atttctccgt taggacatct aaaaatttac gagcagcagg
     3421 agaaagaggc tggtcttttt tccaaatgag attcagtgaa gcttgtaatg gcggatcgag
     3481 tgggatgaat actttttccg tcccataagt attgatgatc ccatctagag ctaatgcacc
     3541 aaaagtcccg ttatcgacca tcaaagaagc attgtacaat aagttatatg ttccgacgat
     3601 cgataactga tctatgctga aacctagcca ttcagctagt tgactgtcca ctgacgtttg
     3661 ccgggaaatg atcaaaggaa cagttttcag ttcctgcgga tggatgactg tttgctttgc
     3721 taaaggatga ttccgatgta tcaaaagtcc ccaacgatct tgggctggca gtcgaaatga
     3781 ctggtatttt tgtttgtttg tcggttcgat tacgagtcca aaatcaagta atccgctatc
     3841 cagtttgtct atgatatcat ctgcattccc gctatataaa tgaaactgga tatcaggatg
     3901 cgccttgatc aacgaatgaa ggctatcaga aatgaaagag attgctttag tttcgcctcc
     3961 gccaatgtag atttctccag agatgattgc gtcatttgtt aaattgttcg ccgttttttc
     4021 taccaacgac aagatttctt ttccttttga ataaagatat cgaccagaat ccgtcaactg
     4081 tatctctcgg gagcctcgag tgaaaagtgt cacaccaagt tcttcttcta aatctttcag
     4141 ttgttttgag agagtaggtt gcgtgatatg cagagatttt gctgctcggc tgatattttt
     4201 ttctctagct actgctaaaa aatacgtcag tatacgaata tccatttttt ccctcctgtt
     4261 tttaaaaaag cataccacta agtatagtta aaaggaatag ttaagtatgt tttataagta
     4321 ttagacatca taagccgtcc gttataaagt aaggataaaa ggagggagca aaatgacaag
     4381 cctcatcgaa aaacaaatcg tcaataaaga gattcgagta acggatccgc tctttgaagt
     4441 gatccatcag atccaagcgg aaaacgaaga gcctttagca gcgctgaata ctgggtacca
     4501 tgaaccagca gatatccgca aacggctaga aaatatcatt agtgacaaag tagatgatac
     4561 ggtgactgtt ctgctgcctt tttatacaga tttcggcaaa catattagta tagggaaaaa
     4621 tgtctttatc aatcgtcaag tgatgttcgt tgacttaggt ggcatctgtt tagaagattc
     4681 agtattgatt ggtcctcgtg tcaacctcat cacggtgaac catctaaccg atccaaaaga
     4741 acgaagaggc ttgtcagtaa agccaatcca catcaagaaa aatgcttgga tcggagcagg
     4801 agcaactatt ctgccaggcg taacgattgg cgagaatgcc attgttgcag cgaatgcaac
     4861 tgttacgaaa gatgtcccag acaataccat cgttgcagga atccctgcca aaatcgtcaa
     4921 acctgtcgaa cggattattt gaacagtaag atgaaataag agatagtgag tataagcaag
     4981 agatttttca aaacaggagg agaatagaat gaatgattta tcagaaaaag tgattgtcat
     5041 catgggcgct tcaagcggaa tcggtgaagc aacaacgaag ctattagcag aaaaaggggc
     5101 taaactagtt atagctgcac gccgagaaga tcgcctaaaa gcaataaaag aatctttacc
     5161 agaagctgag ctatatattc aaacagcaga tgtaagagat tttgcacaag tccaagcagt
     5221 tatcgattta gcgatggaaa agttcggtcg aatcgatgta ttgtacaata atgcaggaat
     5281 catgccaacc gctccattag ttgaaggtca ccgtgatgaa tggcaaaata tgcttgatat
     5341 caacatcatg ggtgtgttaa atggaatttc cgccgtactt ccaatcatgg aaaaacaaaa
     5401 atctgggcat atcatttcaa ctgattctgt ggcgggacat gttgtttatc ctgattccgc
     5461 cgtttattgc ggaacaaaat ttgctgtccg tgcaatcatg gaaggattac gtcaagagca
     5521 acggcaaaat aacatcaaat ctacgatcat ctctccagga gctgttcaaa ctgaattata
     5581 tcaaaccatc agcgacaaaa aggctgctct tgaacttcat gaagcgcaaa aagaatgggg
     5641 attaacatct gaagatatag cttctgctgt agcatttgcg atagaaacgc cagaccggat
     5701 gtctgtcagc aacatgatta tccgtccgac gacacaggaa gtataatcca taacggataa
     5761 tatccagaga gaattacttg ttaaatagtt attcaaacga ccatcaattt aaggcgtgaa
     5821 tggagaagaa tattcttcat ttacgtcttt ttatgcagaa aaagcaaatt tattaaaaag
     5881 aaacaaatgt tatttaaata tggacattga cttgctttta cttttttagt tacactgtta
     5941 tttaggaaaa gtttcataat tcaaaggaga aaaaagatga aagcaaagaa tttattattt
     6001 agcgttattc ttttaggaaa tctagcagtt gccaacacaa cagtgctagc tgaagaatcg
     6061 acagaaactg tcgcaactca gacatcgagt tcttcagaaa caggaattga aaacgataca
     6121 gaaagtactt tgcagtcagc tactgtctca gaaaatacaa tttcaaccga agaaccaaca
     6181 actgaaacaa cagaacaaac acaaaccgaa gacagtacat cagcagaagc tcagaacaat
     6241 ccagagaatg cagaagaaat cacgaaccaa ttgacagatg ggcaaacatt gacacatacc
     6301 catcaagcag atttgtctga attcgaaaac tatactggct atgcttttta tgctcgctca
     6361 tctgctaatt ctcaacaagc gttcattgat agtattgcct caactgcaca aagtttggca
     6421 agtgccaatg acttatatgc atcagtcatg attgctcaag ccatcgttga aagtggctgg
     6481 gggaacagta ctttggcttc tgcccctaat tacaatctat ttgggattaa aggaagctat
     6541 aatggccaat ctgttacgat gcctacttcg gaatatgtga atgggcaatg gattacggtg
     6601 aatgccgctt ttagaaaata tccttcgtac aaagaatcat tgcaagataa cgtaacggta
     6661 ttgaagacga cttcttttca gccaggcgtt tactactatt ccggtgcatg gaaaagcaat
     6721 acgaactctt acaaagatgc tacggcttgg ctaacaggtc gttatgccac tgcgcctaat
     6781 tatggttcta cattgaacaa tgtaatcgaa acctataatc tgacacagta tgatacagca
     6841 ccaacttcaa cttctccgat gtatcgtctg tataaccgtc atacaggaga acatttatat
     6901 acattgaatg ccggagaaaa aaattatctg ccaactgtag gctgggaata tgaagggatt
     6961 gcttggcaag caccaaacag cggacaacct gtctaccgtt tatacaatcc ttacagtgga
     7021 gaccatcatt acacaatggc acaatctgag atcaactctt taacaaaaat cggttggcgt
     7081 tatgaaggtc taagttttta ttctggtggt tccaagccaa tttatcgact atttaatcca
     7141 aatgaaaaaa ctggtaccca tcactatacc ttgagtgcta aggaaagaga ttggctaaca
     7201 ccgatgggct ggagatatga aggaatagga ttttatggtt attaaaaaga ggtagtgaaa
     7261 aagctgccct gcatcaagta gtaagaacag ccaaacaaaa acagattttc atgttttttg
     7321 tttggttgaa cttattaccg cagatgcaag tttttgaaca ccgtttactc aaaaaaggtt
     7381 gtgacaacat cttgtcataa cttttttatt actgtttaag catactgatc caatttctaa
     7441 tattcgacgg gattcttgca aaaggcgatt tcggaaattt ttggcggtgt aaaattatct
     7501 actgcagaag agctatcttc tatagtttga taagaatcaa taatcttggt tttagcgttg
     7561 tgctttcttt atcatttttt ccgttcattc tacaaaaaat ttcttttctc tatacttttc
     7621 tattgagagc gtttttttta ttacacagct gattctttga cttttttctt tttttattct
     7681 attattgaca agtcaatcgt tgacgagtca attattagaa aggaggaaat tatgtcttta
     7741 gaagagatta gtcacctttt atatcaaatc aaattagccg atcaaaaaat cgtccagatc
     7801 tttgaagaaa atatcggtat aagtcttact agatacgaga tcatgatgag actgaaagaa
     7861 gaaaatccat tgctacaaag cagtttgcaa gaaaagttgc agattgatca aggagcagtt
     7921 actcgtcatc tgaaggtatt ggaagcaaat ggctatgttt cgcgtcagag aaatcctcag
     7981 aacaaccggg aagtgtatgt agcgctaaca gaaaaagccc agaagaaaat caagcattgt
     8041 gaaatggacc ataaagatac aggttccgtg ctatccccgg ctttcagcga tgtagaaatg
     8101 aaacaatttc taacactttt agataaattg aatacccaac tttgcaatat aacaacagaa
     8161 acgaaagagg ttaataataa tggaacaaaa aaatgatttt tcaacaatta tgactggtag
     8221 aagatccgtt cgcgtatatg atgaaacata caaaatccca cacgaagaaa tgctggaaat
     8281 gatcaaagaa gcgacgactg caccttcttc tgtcaatatg aaaccatggc gctttgtggt
     8341 cgctgaaagc gaggaagcaa aagaaatctt gcgtcctttg atccgtttca atacgagaca
     8401 aaatgatact tcttctgcaa tggtcatgat ttttggcgat atgcaatgtt acgaatatgg
     8461 cgaagaaatc tacaaccaag cttacgaatc agggaagatg ccaaaagaag tcagagatca
     8521 acaattagct gcgatcattc cttattacaa gagtttttca agagaagaaa tgaacgatgt
     8581 agtcaaagtc gattctagct tagcggctat gcaattcatg ttagttgcgc gtgatcatgg
     8641 ctatgaaaca aatccaattg gtggattcga agcagatcag ttggcagagg catttggttt
     8701 ggataaagac cgttatgttc cagttattat tttatcaata ggaaaagcgg tagaagaagg
     8761 ttacgagtct gttcgtttag caccagaaaa gatcactatg ttcgaataaa aggttttcag
     8821 ttactattca gtagctattg ctttttttga atcgtgtgtg gtaacctaat aaaaaaggag
     8881 gtcggaaaaa tgactgcatc gatgaaatta cgtttcgaac ttttgaataa caactaaata
     8941 aattcaaaag acatgtataa acaacaattt catggatgga gtatgtccat tttttcgaac
     9001 gacagtagaa taggtgaaag ctatgacaga tttggtgatt tagtcatagt tatttcgttc
     9061 agagtaggaa aagatgtgat gcagtttttt tgcaaacaga tttttctgct cttttttagt
     9121 atcgataatt gttgttttac atgaaatagg agtgtaaaac atatggaaaa tttagcagta
     9181 aatataacaa atctacaagt cagctttggc aaccagctag aactatctat tgattctctt
     9241 cgtgtctatc agcaagatcg tatagggatc ataggagaaa atggtgtcgg taaatcgact
     9301 ttgctcaaac taatagccgg tgaacttttt cccgatcatg ggaaaatcca aacagagatc
     9361 accttcaact acctgcctca attaacctat ctcgctgaag caaaggacct aaatttggaa
     9421 ttagccagtc atttccagtt aagactggaa gaaacttcag agcggaaatg gagcggaggg
     9481 gaagaacgaa agatcgagtt gatacgtctt ctttcttctt atgaacaagg gatgcttcta
     9541 gacgagccaa caacccatct agatagaaaa agtattgatc gactgattga agagcttcgt
     9601 tattattatg gtacgctggt ttttgttagt catgatcgct attttctaga tgagctagca
     9661 tcgaaaatct gggaagtaaa agacggagaa atccgagagt tttcggggaa ttatagtgcc
     9721 tatctcactc aaaaggaatt ggagaaaaag actcagctac gagaagcaga gtcgatcatg
     9781 aaagagaaaa aacgattgga aaaatcgatc caagaaaaga aaaaacaagc ggaaaagttg
     9841 gaaaaagtgt ccagtaaaaa gaaaaagcaa caaatcagac tggatcggtt gtcttcctct
     9901 aaacaaaaag acagtgtaca aaaagccatc caaaagaatg cgaaaacatt agagagaaga
     9961 ctccaaaaaa taggagaaac aaccaagccg cagcagatga aacaaatccg ttttccagta
    10021 ccaaaatctc ttgaactcca cagccgctat cccatcatgg gacaaaatgt ccaattggaa
    10081 agaagtggga gaacattact ggtaaatggt gattttcagt tttctttagg taaaaaaatc
    10141 gcgattgtcg gtgaaaatgg ttcaggtaag acaaccttat tagaacatat ccgcaaacaa
    10201 ggagaaggaa tccttctctc tccgaaagta agctttcaag tatatcagca aaagggttat
    10261 caaatgacat ctgaagaatc catcattcgt tttgtcatga gacaaacaga gttttcagaa
    10321 tcgcttgtcc gtagtttgct gaatcactta gggtttgctc aggaaacttt gacgaaaccg
    10381 ttatgtacat taagtggggg agaagcgacc cgtctgacga ttgctttgct ttttactaag
    10441 ccaagtaatg tgttgctgtt agatgaaccg actaatttta ttgatatggc aacgatcgaa
    10501 gctttagaga agctaatgca aatatatccg ggaacgattt tgtttacttc tcatgattct
    10561 tactttgtcg agcgtacggc tgatgaagtt tatgaaataa aagggcagaa aataaaaaaa
    10621 gtacttacga gaaattttta aatgtgctac gatgattttc gtgacaaatt taaaggagtg
    10681 gtacagattg cctacaaata aaaaagaagg aataattttt acaacaatga tgtgtttttt
    10741 gatggtcttg gggatgagcg cgtataattt atggctgcat caagatcttc atttatcaag
    10801 tttgttgatt ggtctagtgc ctggattcgt ggtggctttc attttggatg tcttcatcat
    10861 cggtgtgctg gcgaaaaaaa ttgcctttaa actaccagta aatccagaaa gcaaggtcca
    10921 aatgatctta actatttcat gcttgatggt tttaggaatg gtgacatgta tgtctttatt
    10981 cggtgtgatc atggaacaag gaatacctga tcaattattc tctgcttata tgaatgcttg
    11041 gaaaatgaat gtgattgccg ctttaccgtt gcagttgatc atcgttggcc cgctttcaag
    11101 aagtgtgcta aaagcaattc aaagtaatga atgatggaac actgttattt gaaaaaaggc
    11161 cttggcatgc tttttgtcat agtctcttga atgcagttct agaaaaatag tgatccgaac
    11221 gataggtttt ggcctgtcgt tcggatcgct ttttcttttc caactgtatt gctcttttat
    11281 tgaagataat ttgctcttca atagatattt ctagaaaggt tctgtttatc ccttactcaa
    11341 aaaactgctg catttgttct cttacttgct gacaccattg gagttcctgc tgacaaagca
    11401 taattctgcg ctggatcaca acataacgtc caaattcttg tttcttgtct agcgagaggt
    11461 cattcaattt tttttgattt ttttgcatga ttttgttcag ctgttgttct ctagtgaata
    11521 gcagtccagt agctgttgtt ttatcgagcg tcgaaaaagc atagattttt gctagaaatt
    11581 catctttttg cgtaggagcg ggagtttcct ctttgatcca ttcctctaca agagcacgtc
    11641 cttcatcagt caagtggtag atcttttttt gagagtgctc gtctttgtcc aaaacggtca
    11701 cataaccctg ctggtctaac ttggcaagcg ttgtatagat ctgactgtga tgggcttccc
    11761 aaaaaattgt gatcagctgc ttcaattcat acccagaacg tggtttggat aaaagcacgc
    11821 ataataatat ataggctaaa gtattcataa aaatcctacc tctcgtctta ttttctttat
    11881 tatatcacag taaagtttcc acgttttaca tgtgcatatt gacatgtaaa ataaatcatg
    11941 tatgatgcta atagagagaa agggatggaa atattggaga atcaaataga tatacaagaa
    12001 caaacaaatg aagaaacaaa ggggaaaagt tcagcgctag ttgtagcagc tacctgtatg
    12061 atcgctttta tgggagtggg tctagtagac ccaatcttaa aaacgattgc tgcacagtta
    12121 aacgctacgc cagctgaaac gaccctgtta tttactagtt acatgttagt cactggtgtg
    12181 gtgatgctat ttacaggttt cttatctagt cggatcggtt tgaaaaaaac attgatgaca
    12241 ggattgttta tcatcgttgt attcgctggt cttggcggat tatctgggaa tatcggaatg
    12301 ctgattgggt tacgtgccgg ctggggtgtc gggaatgctt tatttgtctc tactgcttta
    12361 gcaacgatcg tcagtatttt agttggtaac acagaaaaag caatcatgat gtatgaagca
    12421 gcacttggct gcggaatggc tgttggccct ctagtcggtg gggtcttggg cagtggttca
    12481 tggcgttatc cattttttgg ggtagcattt ttgatgtttt gcggttttat agccctagcg
    12541 gtacttttac ctgaaactga gaaaccaaag agaaaagtag gcctttggga aggtgtcaaa
    12601 gcgttaagca atcataaact tcgttcagtt ggactaatcg cattgttata taatttcgga
    12661 ttctttactt tacttgcgta tgcgccattt ttattgattg gctattctga attagaagtc
    12721 ggttttgtat tttttggctg gggtgtgctt ctggctattg cttcgatttt cgttgcaccg
    12781 gctcttgagc ggaagtttac gacttatcgg acaattttag cagcatttgt cttattcatt
    12841 atttgtttgc tgttattagg agctggaact tctatgccag tccttgtacc gatcagcatt
    12901 gtgttagcag gatttttcca aggaattatc aatacactgt tgacgacgat tgctatggaa
    12961 ataccaggtc tgcaacgaaa cgttgcttcg tccagctata gttttgtccg ctttttcggt
    13021 ggagctttag caccatttat tgcaggtgtg attggtgaac atattggtga aagctatgcg
    13081 ttttattttg caggtgccat tgttcttatc agtgtatgtt gtatcattta tcaccgtaat
    13141 tattttgcta caaatgaaga agaataagaa atcagaggtt gtgaaaaagc tgtcctgcat
    13201 caagtcataa gaa
//
LOCUS       DADVXU010000293         7943 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.guided.115, whole genome shotgun sequence.
ACCESSION   DADVXU010000293 DADVXU010000000
VERSION     DADVXU010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.guided.115"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            128..1564
                     /locus_tag="KB953_003125"
     CDS             128..1564
                     /locus_tag="KB953_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBB6868003.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKSSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4185
                     /locus_tag="KB953_003126"
     CDS             1753..4185
                     /locus_tag="KB953_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBB6868004.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQLSFVPI
                     DSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQDYELPT
                     VDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEVQPAVG
                     VKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIEAQPNH
                     PDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRAKPHEV
                     KLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAATGVRNI
                     SGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMARAAGI
                     HMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGDMLFLP
                     MGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQPQDELF
                     EEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKVFIEQE
                     ETSETLPDDNDM"
     gene            4415..5482
                     /gene="rlmN"
                     /locus_tag="KB953_003127"
     CDS             4415..5482
                     /gene="rlmN"
                     /locus_tag="KB953_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBB6868005.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5675..6778)
                     /locus_tag="KB953_003128"
     CDS             complement(5675..6778)
                     /locus_tag="KB953_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBB6868006.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7330..7878
                     /gene="aac(6')"
                     /locus_tag="KB953_003129"
     CDS             7330..7878
                     /gene="aac(6')"
                     /locus_tag="KB953_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HBB6868007.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2658 a         1357 c         1724 g         2204 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaagttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaata tgagaagaaa aaagcggtga gccagcaaag ggaaaatact
     2521 gctgatgaat ggcaggaacc agaaaataca gagccggagc agctaagttt tgttccaatc
     2581 gacagttttc aagaaaatat ccatcctgcg aatctagaga aaccagtacc agatacacca
     2641 aaacagacca atacagcaga aggtttttca gatgagttgc cagaggatga cggtacctca
     2701 ttagaatttg agattgaagc agaacaggaa aatcaagact atgaattacc gacagttgat
     2761 ttgcttgatt ctataccaac tgtggatcaa agtgatgagt ataaaaaaat tgaaaaaaat
     2821 atcggcgtat tagaacaaac tttccaaagt ttcggtgtcg atgccaaggt cgtcaaagcc
     2881 agcttaggac catccgtcac aaaattcgaa gtccagccgg ctgtaggggt aaaagtaagt
     2941 aaaattgtga acttaacaga tgatatcgcg cttgctttag cggcgaaaga tgtccggatg
     3001 gaagcaccga ttccaggtaa atcactgatt ggtattgaag taccaaatgg aaagatcagt
     3061 atggtttcct ttagagaaat catcgaagca caaccaaacc acccagataa attactagaa
     3121 gtacctttag gacgtgatgt ttctggacgg gtacaaacgg ctgatctttc gaagatgcct
     3181 cacttactcg tggctggttc gactggtagt gggaagtcag tagcaattaa tgggattatc
     3241 acaagtatat taatgcgtgc taagccccat gaagtaaaac tgatgatgat cgatccgaaa
     3301 atggttgaat tgaatatgta caacggcata cctcatctgt tgacgccggt cgtgacgaac
     3361 cctagaaaag cagcgcaagc cttacaaaaa gtagtacaag aaatggaaga acgatatgaa
     3421 aaattcgcgg caaccggagt aagaaatatt tctggttaca atgaatttgt tcaacagaaa
     3481 aacttggaaa acggaacgaa acatccaacc ttaccattca tcgtagtgat cgtagatgaa
     3541 ttagctgatt tgatgatggt agccagcaat gaagttgaag atgcgattat tcgtttagcg
     3601 caaatggctc gggcagcggg gattcatatg atcctagcta ctcaacgtcc aagtgtggat
     3661 gttatcacag gaatcatcaa agcaaatgta ccttccagaa tggcatttgc ggtatcaagc
     3721 ggtacagatt ctcggacgat cattgattct aatggggcag aaaagctctt agggcgagga
     3781 gatatgctat tcttgccaat gggtgagaat aaaccgatcc gtgtgcaagg agcattcatt
     3841 tctgaccacg aagtagaacg ggtcgttcaa tttgttacag atcagcaaga agctcattat
     3901 gaagagaaaa tgatgccgac agatgaagtt gaaacgacag gtgctccaga acagccacaa
     3961 gatgagctgt ttgaagaagc aaaagcactt gtcgttgaaa tgcaaacggc aagtatttcc
     4021 cttctccaaa gaagatttcg gatcggttac aaccgagcag cacgtttagt agatgaactg
     4081 gaagctcatg gagttgttgg cccgtcagaa ggaagcaagc ctcggaaagt attcattgag
     4141 caggaagaaa catcagaaac gttgccggac gataatgaca tgtaaaactt gtgtgatgaa
     4201 gggttgaaaa acgaataacc aataaaaaga caaggcatga tgataccaaa agaaaaaatg
     4261 gtttttttcc atcttttcta atacggtatg atgtcttgtc tttttagttt ttgtgagatt
     4321 aaagttctat tcgatttatt tgtgaagtat tgcattaagg aaatgaatca tgtatagtat
     4381 gtagcgaaac tttgagaaaa agagggaaaa tactttggaa aaacagtcca tttatggctt
     4441 gacgaatgaa gaattaatca attggtttat agaaaacggc gagaaaaaat tccgagctgc
     4501 ccaagtttgg gaatggttgt atcaaaaacg tgtaagtaat ttcacagaaa tgacgaatct
     4561 atcgaaacaa ttgatcgaaa aattatcgga gcattttatc atcaatcctc taaaacagat
     4621 ggtcgtccaa gaagcttcgg atggtacagt aaaatatttg tttgaactcc ctgataaaaa
     4681 catgattgag actgtactga tgagacaaga atatggcttg tcagtttgtg tgacgacaca
     4741 agtaggatgt aatatcggct gtacgttttg tgcaagcggg ctgctcaaaa agaaccgtga
     4801 tctgactgct ggagaaattg ttgcacagat catgatggtc caacattatt ttgatgagcg
     4861 caatcttggc gaacgcgttt cacatgtcgt tgtaatgggg atcggtgagc catttgataa
     4921 ttacgacaat gtaatggact ttttgcatat catcaatgat gccaaaggat tggcaattgg
     4981 tgctcggcac attacagttt ccactagtgg attagcacac aaaatcaagg aattcgctaa
     5041 taacggcttg caagtcaatc tggctatttc cttgcacgcg ccgaataatg aggtgcgaac
     5101 atcgatcatg cggatcaacc gaagtttccc aattgagaaa ttgatggaag ctgtggatga
     5161 gtatttagag aaaacaaacc gtcgaatcac gtttgaatat atcatgttga atcaagtaaa
     5221 tgatagacca gagcatgcgc aacaattagc agatctatta aaagataaga aaaaattagc
     5281 atatgtcaac ttgattccat ataatccagt cagtgaacat gatcaatatt ctcgcagtcc
     5341 aaaagctgat gtattgagat tttatgatgt attgaagaaa aacggcgtaa actgtgtgat
     5401 acgtaaagaa catggtacag atattgatgc agcttgcgga caattgcgaa gcaagcaaat
     5461 gaaaaaaaca gttgccaaat aatcgtatcc tactcgtttt ttcaattacc ataaaaggag
     5521 cagttttctt aattaaggaa ctgttcaact taaataagag aaaagattct atagcgaaag
     5581 ctataaagct ttttttgaca agaaaaaccc tcatcaagca aagaaatgtc ctcaatttga
     5641 tgacagcctg cttgatgggg gttttagcaa ttatttaact gattttctta tatccttgct
     5701 ggctttcttg tgtagcatga taagtttcta cgtcaagaat ggcctgttta aatttttctc
     5761 gggtgatttc ataaggcaca tgtttaatat catttgttgc catggcttta tctaagattg
     5821 cttctaaatc ggtcgtgaca ttgagattca tgtcggttaa cgatactgga agttggttct
     5881 ttttcataaa cacatagatt ttatcaaaca aatcgaactg ctgatccatc atcaataagg
     5941 tcagtacacc ataactgaca gatgctccgt gaaggtatcc ctccattttt tctagaacag
     6001 tcgtaccgta atagaatgag tgtgccatag cagaattata gttattgtta actaaaacag
     6061 aaaccaatcc ggtagtaaca atgatatccg aaacgacttg ttcaattgca tatgagggac
     6121 gatcgtcggc actatctttc aatgcttgcg taccataagt caagagacga tcaatgcaca
     6181 tttttccgat ttggattcct aatctatccg tgtagcctag atcatcgcca cgagcagaaa
     6241 aagtagattc gacttctttg gataaagcat cgccaatacc tgcccataag tatttatcag
     6301 gtgcttcggc aataattgct gtatcaatga aggtgtgtac aggtggtttt gaaggaaaga
     6361 agagtccgtc cattacgcct tcttcgttat agatgacaca taccgcagtg acgggcgaac
     6421 agttcgaacc aatagtggga aaggtgaaga ctggtttagc tagtttttca ccaatgactt
     6481 ttacagtatc gcaagcacgg ccaccaccga ctgcaaacag gacgtctgct ttttggacac
     6541 tttcatgctg gatcaactgt tcaatatttc cataagttga attacccccg taccagagac
     6601 tggcgacaac ctctatttcc tgtgtcaaag ctacttttat tttgttttct gcctttgaaa
     6661 gtgcagtctt ccctccaatc agcactactt ttgtcccata ttctttgacg attccatttg
     6721 ctttttcata cgcatctgtt ccaatggtat aatttggtaa gaaaacactt tgactcatat
     6781 tcatttcctt ctttcttcgt tgaaacgggt taaaatttta aagagtgatc gatctgactg
     6841 cggctgtaga tggagtagag gtgaacctaa gacaggataa gtgatgaata aggtggcatg
     6901 aacaccttga aatcatcaat aagtatccta aaaacgaaca agtacaaaaa cattgaaaac
     6961 gacaataatg aatttttgtt cctcttagta tttctaccga ttccctttgc ttttttgtaa
     7021 ttatctaaaa attctaaaaa ttaagattat tatatgaaaa caggttttat ttgtcaattc
     7081 ataattggtt ttttttaaag caaaagataa tctatatcat gatgaacaaa cagagttttt
     7141 tcctccaatt gatggctttc ctgatctttc ctattttgaa taaagtgttt ctgtataatt
     7201 ttttatttta taagtaagat aaataatgaa agaatagaga tactgattgg tagctaaaga
     7261 tttttgagcg agaacatttc gttttttcaa aaggactgtt agaataagca agaacaaggg
     7321 ggaagacgta tgataatcag tgaatttgac cgtaataatc cagtattgaa agatcagctt
     7381 tctgatttac tgagactgac ttggccggaa gaatatggag acagctcggc agaagaagta
     7441 gaagaaatga tgaatccaga acgaatcgcg gtagcagcgg tagaccaaga tgagttagta
     7501 ggatttattg gtgcaatccc tcaatacggt atcacaggtt gggaattgca tccattagtt
     7561 gtagaaagct cccgacgaaa gaaccaaata ggtactcgat tagtcaatta cttagaaaaa
     7621 gaagtagctt ccagaggagg aatcacgatt tatttaggta cggatgattt agaccatgga
     7681 acaacgttaa gtcaaacgga cctgtatgag catacatttg ataaagtggc ttccatccag
     7741 aaccttcgtg aacatccgta tgaattctat gaaaaattag gttataaaat cgtaggtgtc
     7801 ttaccaaatg caaatggctg ggacaaaccg gatatttgga tggcaaaaac gattattcct
     7861 cgaccaaatt ctcaataata aaaagaggtt gtgacagaag taatcagctt caagaaataa
     7921 gaaggaattt acgaaaatta ctt
//
LOCUS       DADVXU010000294         5366 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.guided.141, whole genome shotgun sequence.
ACCESSION   DADVXU010000294 DADVXU010000000
VERSION     DADVXU010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.guided.141"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            322..1014
                     /locus_tag="KB953_003130"
     CDS             322..1014
                     /locus_tag="KB953_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBB6868008.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSWKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            1341..1838
                     /gene="dfrG"
                     /locus_tag="KB953_003131"
     CDS             1341..1838
                     /gene="dfrG"
                     /locus_tag="KB953_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HBB6868009.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(1910..3862)
                     /locus_tag="KB953_003132"
     CDS             complement(1910..3862)
                     /locus_tag="KB953_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HBB6868010.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3869..4105)
                     /locus_tag="KB953_003133"
     CDS             complement(3869..4105)
                     /locus_tag="KB953_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6868011.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            4507..4797
                     /locus_tag="KB953_003134"
     CDS             4507..4797
                     /locus_tag="KB953_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6868012.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
BASE COUNT         1821 a          877 c          929 g         1739 t
ORIGIN      
        1 tttcataggt aaaagaagca tgacaattta tgacaaatcc gcgttttttg taataaatac
       61 agtaatctat gaaatgaaaa gggttacgtt taataaaact tgcttttttg tttttgttca
      121 ccataaatat ttatttaaga aagtattaac ctgcattttt ctctataaaa aagatttttt
      181 aaatagataa aaaaggagga ttaaatgagt atttgatagt atttatatta aaattacata
      241 tgtaatttaa aaacgcttga cattgtgttt gagatcactt acttttagca tgtaaaagta
      301 ttggattaag gagctatgca aatgaaaaag aaatttatta ctggtgggat tgtcgttgca
      361 tttctatgtc taattggcta cacaggcacg cggtacgcaa atgaaattat tttgtggggt
      421 ggagagacag atattgatac aatcaatgag aatctgacac agcttgatca tacattgaca
      481 gataaagagc aagcgattac agatcttacc gctcgactag cacaaaaaga acagacattg
      541 caagattcaa aggcagatgt ccaagaatac caaacaaaag taaatgaatt agaaaaccaa
      601 aaaaatcaat cggcagcaga aaaacaaaac ttagaaagtc aattagtcag caaggatgca
      661 caactacaaa gcaaacaaaa tgaaatcaat gtcaaactag atgagatcaa ccaaaaaaat
      721 gatgaaatca atcatctgaa tgcagattgg acagcaagac tacaagaagc acaagcaaaa
      781 ctttcagaat tacaaaatac tgtaaatggt ttgaatcaac aaatcgcaac actgacacaa
      841 gaaaaaacaa atgtagaaac tcagttaaca gatacacaaa agaaattaga cgaagcaact
      901 gctgaaaact caagctggaa acaatatatt caaaagttag aaaaagcaaa agatgaagtg
      961 gaagatacag ctgataaatc tcagcaaatt gttgaagaac atttgcgtaa ataatctttc
     1021 ttaacaagta aacctaaatt agtaaagaag caacagtaat ctatactaag tagttcagtt
     1081 ttggagtaca aaatgacaaa atgtacttca aaatggacta cttttttcat tttaacttac
     1141 ataccaaatt acatacaaaa tgatttataa aatgcaattg aaaatgataa ttgaatgaaa
     1201 atttgtgatt ttgtggtatt atgataatat aacttataga aaaaattgct tattcaacta
     1261 acgggcagga taatgagaaa aggttttttg atagaaataa gaaattagat ggtaaattag
     1321 ttaaaaaggc tgtgttataa atgaaagttt ctttgattgc tgcgatggat aagaatagag
     1381 tgattggcaa agagaatgac attccttgga ggattcccaa ggactgggaa tatgttaaaa
     1441 atactacaaa gggacatccg ataatattag gtaggaagaa ccttgaatca atcggaagag
     1501 ccttacctga cagaagaaat attattctga cgagagataa ggggtttacc tttaatggtt
     1561 gtgaaattgt tcattcaata gaagatgttt ttgagttatg taaaaacgaa gaagaaattt
     1621 ttattttcgg aggagaacag atttataatt tgtttttccc ttatgttgag aaaatgtaca
     1681 tcacaaaaat acatcatgaa ttcgaaggag atactttttt tccagaagtg aattatgagg
     1741 aatggaatga ggtatttgcc caaaaaggga taaagaatga taaaaatccg tataactact
     1801 attttcatgt atatgaaaga aaaaacttat tgagttaacg ggtgcgttaa ttgaagaaga
     1861 acagtaaaga taaagcacaa gagcttggga ttactccaag ctctttttgt catttgaaat
     1921 aaattatatc tttccaatca aattgtttat ctgtaatccc taatctttga gcaggtgtca
     1981 aaaccttttt atccgctgat ttaaaagggc gacaaaagtt gtagtaagtt cttagaattg
     2041 taatcgcaaa ttgagcatac ttcggattga agttagcgta aatatagctc ttcttatcac
     2101 cacgagctgt tgttaatggt ctttcaagag atgatatata acgtcttatc tgttgaataa
     2161 agctattggt cgaatggtca ttaacattta atagcatctt tgcgatttcg tttggttcca
     2221 gagcagataa atccgttcta caatcaactg aataaaatcc tttatcctta gtggctaatg
     2281 gatgtttgat agggttatcg gcatatttgc gatatctttc tccatcttta ctaatagcct
     2341 cttcgtggaa ttgatgactt gttagtaatt cagttagata atgagatgcc aaagtccgta
     2401 gaaactttgt tttgaatcct ctgatatctc cccaatcgag cagttctgct tttgcttgtc
     2461 cgaattcttt taagcattgt tttctgcttt tcgtcttgtt aacttggcaa ataaaatgat
     2521 gtgcatcgct caaccttaat tctttcgtga atactctata aaaggcgttt ctaatagaga
     2581 agtcatcatc actaatcatt cgccattcgt cggcattaac catttgttta attaaccaat
     2641 agtgagcagt tgtagtataa ggagcatcaa catttaaacc atcgacaaat ctactcctat
     2701 tagtaatttt ccctaattcg tgaagataag cattcctatt ttcgctatcg ttagcggatg
     2761 gttcttgtgg ataataagac caatctaacc tatcattttt acgactaaaa gtattgagat
     2821 ggtcttcttt gaattttctt gtatcttcat ttaattcatc cattgaaata ttccaatcat
     2881 aggcaacatc agaacggaac acatatcttg ataagacctc agcagataca actatatacg
     2941 tttgcatatt aaggtcttca aaccccgtat atttctttga accttgtcct ttcttacgga
     3001 cgttatttag gtagtagtgc atcttgtccg tattaagcca catctcgtta aatttctttg
     3061 tttgaagggg ttgtgtctca tatcgttcta aaaactccaa acaacgccta tacagccact
     3121 ccagcttatg atagtaggtt ccaactccaa ttcctaaaat atcacaagta cgactgactg
     3181 aaacccttcc aacaaccatc ttagcgaaca ttggaagaat ggtgttcttt tgttggtgat
     3241 aagtagtggt ttcttctctt tttggtagaa cattagtgaa cttcttacac gccttacatt
     3301 gatagcgttg agatttgccc ctacttttcc cccttttgta aaattgcttc ggctcattga
     3361 atggggttga ttcttcttcc gagcagcctt ctttatgaaa ttgataatct ggctcaacat
     3421 cttggatgct attgatttct ataaggcgtt taatttcttc aacaacagac caattagata
     3481 aaggggttac tgtacagttt tgcgatacac ctctgccaat aggattgtca ttgcagtaca
     3541 tacctttatg acctttatct ttggaactac cagtcatttt atatcgactt ggtttccctt
     3601 ttacatcttt gaatttgtgc tgttctttcc caaaattaca acaaaacgga ttgatgcagt
     3661 aattatattg aatgggatat tcagaaccgt tccatttaaa agacgttggc aagaacaaca
     3721 ttttgttata ccttgttgca aatcgttttg gagttaataa atcatattgt ttagccctat
     3781 cttttaattc ttcgtttgaa atcggaattt taacatctac aatttcatct tccttcgtgg
     3841 ctaatctttt aagcttggtc attgtctatc aacccactct atctttgata cccatttttt
     3901 caacaatccg cttattactt cgcttgttgg caatccattc aataaacttc ttatcgtcta
     3961 aaatgttcaa taagaattca tccactgctt cgctttcagt acgctctgca tattcagcgt
     4021 attgcttaac tatcaccctt acttgctctg atatttccca atccacagac ttagcgttct
     4081 tattttttgg ctcaagaaat tgcataggaa aatgcacccc tttttgctta gtaaattgta
     4141 accattttaa cacaccaatt tgcattccta caataattat ttcacgttcg catttttata
     4201 tacattttaa cttacatttt gtggtgtaaa ttaacaacaa acgttgattt aacaataaaa
     4261 aacgagtatc aaattcagtt gcaatttgat actcatttag gtttacattt tcactttttg
     4321 tactccaaaa ctgaactact taaatctata ctgctgcctc ttttttttag tacatgtcct
     4381 tcatcttcgc aggaagtgcc aatatgattt taagaaaggt aatagacaaa aagtcaaaaa
     4441 gataaaacag agtttcttct ttacaaaggg aatgtatgtt cgtataatgg tattgaggtg
     4501 aaggttatgg agaaaaaata tggaacagta gataagatcc gtactttgca agttagtgcc
     4561 agaccgttga tccgtttcag tttaaatggg gtcaattgtt tgattgcttc ccatagtttg
     4621 aacttcctag ctgaagtaga cgaagggatg aagttagtcg ttacaggtta ttataataat
     4681 agaaagcaat ttgttgtgag atcctatcat gtgttaggga aacccaaaat tgtggtagaa
     4741 tatgagaaaa gtctataccc aagaaaaaag gtccgacaag atgaaagaat aaactagaaa
     4801 acaagcggct gagacaaaaa tgtcctagcc gcttgttttg gaaagatgga tgcagacgat
     4861 gggatttgaa cccacacgag catacgctca caagaacctc aatctatata taactacatg
     4921 tatttaagta tttgtgatga atcactaaac tttccaaact catttgaggg atattgctta
     4981 aagattgaac agatcaattc gaagccctat tagtattcgc tcaatggggg gatcagaggc
     5041 cataaaaaga gactagtcat tactagtctc aggtagtgga attgtaggat tttaaacgtt
     5101 cctggagagt accggtatgt aactcgaaga ggtgattatc atagtcgtaa aagtaaattg
     5161 aagaagcctc gcctttcaca cgagaacgtc ctggttcgac agttaaacct aacatttgga
     5221 tttttgagac aaacgaatct atatcttgtt cgtcaatttt aaaagcaata tgattgtatg
     5281 tctttggcag tttattcgac gaattctgca ttactgcaat ccataggtcc ttaactaaga
     5341 aaaatttttc tggatataat gaaaat
//
LOCUS       DADVXU010000295         4702 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.guided.147, whole genome shotgun sequence.
ACCESSION   DADVXU010000295 DADVXU010000000
VERSION     DADVXU010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.guided.147"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            194..1000
                     /locus_tag="KB953_003135"
     CDS             194..1000
                     /locus_tag="KB953_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HBB6868013.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            1018..1725
                     /locus_tag="KB953_003136"
     CDS             1018..1725
                     /locus_tag="KB953_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HBB6868014.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWLFLHTP
                     LTIIQRRGHEVKEFPEPNSDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKII
                     GQVFADKEPQALYKITNGSIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKC
                     KICYPLPESVDIQEENQ"
     gene            1722..2693
                     /locus_tag="KB953_003137"
     CDS             1722..2693
                     /locus_tag="KB953_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HBB6868015.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            2686..3714
                     /gene="vanB"
                     /locus_tag="KB953_003138"
     CDS             2686..3714
                     /gene="vanB"
                     /locus_tag="KB953_003138"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HBB6868016.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            3720..4328
                     /locus_tag="KB953_003139"
     CDS             3720..4328
                     /locus_tag="KB953_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HBB6868017.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(4394..4567)
                     /locus_tag="KB953_003140"
     CDS             complement(4394..4567)
                     /locus_tag="KB953_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBB6868018.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         1329 a          986 c         1245 g         1142 t
ORIGIN      
        1 gatagaatga ttttctatca gtcctagaaa ttatagagcc gattttgacc gacaggtata
       61 accctgccgg tctttttttg ctgctacagg aaaactacag actggctaca gggaaagtac
      121 agatgcgctt gccataataa caatcgtacc agccacaaat cgtagtttta ttgcaaagga
      181 ggcattcgat caaatggaaa aaagcaacta tcattccaat gcggatcatc acaaacggca
      241 tatgaaacaa tccgtggaaa agcgggcttt tctatgtgcg ttcattattt cgttcacagt
      301 ctgcacgctg tttttggggt ggagactggc ttccgtattg gaggcaacac agataccgcc
      361 catccctgca actcatacag gcagcagcac tgacgtagtg gagaatttgg aggaaaacgc
      421 tcttgccacc gccaaagaac agggagatga acaggaatgg agcctgattt tagtgaacag
      481 gcagaacccc atccccgcac agtacgatgt ggagcttgag caactatcaa atggtgagcg
      541 gatagatatt cggatttctc cctatcttca agatttgttt gatgccgcaa gaactgatgg
      601 agtttacccg attgtcgcat ccggataccg aacaacagaa aaacagcaag aaattatgga
      661 tgaaaaaatt gccgaatata aggcgaaagg ctacacctct gcacaggcta aagcggaagc
      721 agaaacttgg gtggccgtgc cgggaacgag cgagcatcag cttggtcttg ctgtggatat
      781 caatgcggac ggaattcatt caacaggcaa cgaggtttat agatggctgg atgaaaacag
      841 ctatcgtttt ggttttattc gtcgctaccc accagacaag acagagataa ccggtgtgag
      901 caacgagccg tggcattacc gatatgttgg catcgaagct gccacagaga tgtacaacca
      961 aggggtttgc cttgaggaat atttaaaacc agaaaaatga gaaaaggatg ttatgttatg
     1021 gacagaaaaa gattgacaca gcgtttcccg ttcctgcttc caatgagacg ggcgcagaga
     1081 aaaatgtgct tttatgcggg aatgagattt gacggctgtc gctatgcgca gacgataggg
     1141 gaaaaatcgc tttcccattt gctctttgaa acggattgcg cattatacaa ccacaatacc
     1201 ggatttgaca tgatatacca ggaaaacaag gtgttcaact taaagctggc agcaaagacc
     1261 ttaaacggct tattgataag accgggggaa accttttctt tctggttgtt cctgcatacg
     1321 ccattgacaa ttatccagcg cagaggtcac gaagtaaagg agtttccaga gccaaacagt
     1381 gacgagatta aaggggtgga tgcaaccatt tcagagggct ggattgattt aaaagtgcga
     1441 aacgataccg actgcaccta ccaaatatgg gtgaccttag atgatgagaa aatcatcggt
     1501 caggtgttcg ccgacaaaga gccccaagca ttatacaaaa ttacaaatgg cagtatccag
     1561 tatgtccgtg aaagtggcgg gatttatgaa tatgcccagg ttaaacggat gcaagttgcc
     1621 ttaggtaccg gggaaataat agattgcaag ctgctttata caaacaaatg caaaatctgt
     1681 tatcccctcc cggaaagtgt ggatattcag gaggagaacc aatgagaaaa agtgtgggca
     1741 ttactgtttt tggatgcgaa caggatgagg caaatgcttt ccgcgcttta tcgccggatt
     1801 ttcatattat ccctacgctg attagcgatg cgatatcggc agacaacgca aaattggccg
     1861 ctggcaatca atgcgttagc gtaagccata agtccgaggt ttccgaggcg acaattcttg
     1921 cgctgagaaa ggtcggggta aaatacattt ctacccgcag catcggctgc gatcacattg
     1981 atacgactgc cgccgagaga atgggaatct cggttggcac ggttgcgtat tcgccggaca
     2041 gcgttgcgga ttatgctttg atgctgatgc tgatggccat acggggtgca aaacccacca
     2101 tgcacgccgt ggcgcaacaa gatttcagat tggatcgtat ccgggggaaa gaactggggg
     2161 atatgactgt gggagttatt ggaaccggcc atatcgggca agcggtcgtc aaaaggctgc
     2221 ggggatttgg atgccatgtg ctggcctatg ataacagccg aaaaatggat gcagattatg
     2281 tccagcttga tgagcttcta aaaaacagcg atattgttac gctccatgtg ccgctttgtg
     2341 cggatacccg ccatctgatc ggtcagaagc aaattggaga gatgaagcaa ggcgcatttt
     2401 taatcaacac cgggcgcgga gcgcttgtcg ataccggggc gctggtggag gcgttggaaa
     2461 gcggaaagct gggtggtgcg gcactggatg tgttggaggg cgaggatcag tttgtttata
     2521 ccgattgctc gcagaaagtg cttgaccatc cctttttgtc gcagctttta aggatgccga
     2581 atgtgatcat cacaccccat acggcgtact acactgagcg tgtgctgcag gatactacag
     2641 aaaaaacaat caggaattgt ctcaattttg aaaggagttt acagcatgaa tagaataaaa
     2701 gtcgcaatca tcttcggcgg ttgctcggag gaacatgatg tgtcggtaaa agccgcaata
     2761 gaaattgctg cgaacattga tacggaaaaa ttcgatccgc actacatcgg aattacaaaa
     2821 aacggtgtat ggaagctatg caagaagcca tgtacggaat gggaagccga cagtctcccc
     2881 gccatactct ccccggatag gaaaacgcat gggctgcttg tcatgaaaga aagcgaatac
     2941 gaaacacggc gtattgatgt ggctttcccg gttttgcatg gcaaatgcgg ggaggatggt
     3001 gcgatacagg ggctgtttgt attgtctggt atcccctatg tgggctgtga tattcaaagc
     3061 tccgcagctt gcatggacaa atcactggcc tacattctta caaaaaatgc gggcatcgcc
     3121 gttcccgaat ttcaaatgat tgataaaggt gacaagccgg aggcgggtgc gcttacctac
     3181 cctgtctttg tgaagccggc acggtcaggt tcgtcctttg gcgtaaccaa agtaaacggt
     3241 acggaagaac ttaacgctgc gatagaagcg gcaggacaat atgatggaaa aatcttaatt
     3301 gagcaagcga tttcgggctg tgaggtcggg tgtgcggtca tggggaacga ggatgatttg
     3361 attgtcggcg aagtggatca aatccggctg agccacggta tcttccgcat ccatcaggaa
     3421 aacgagccgg aaaaaggctc agaaaatgcg atgattacag ttcccgcaga cattccggtc
     3481 gaggaacgaa atcgggtgca ggaaacggca aagaaagtat atcgggtgct tggatgcaga
     3541 gggcttgccc gtgttgatct ttttttgcag gaggatggcg gcatcgttct aaatgaggtc
     3601 aataccctgc ccggttttac atcgtacagc cgctacccac gtatgatggc cgccgcagga
     3661 atcacgcttc ctgcactgat tgacagcctg attacattgg cgttaaagag gtgataagta
     3721 tggaaaatgg ttttttgttt ttagatgaaa tgttgcatgg cgtccgttgg gatgccaagt
     3781 acgccacatg ggataacttc acaggaaaac cggtggatgg gtatgaggtg aatcgcatca
     3841 tcggcacaaa ggccgtggcg tttgctctgc gcgaggcaca aatccatgcg gctgcgcttg
     3901 gctatggctt gcttttatgg gatggatatc ggccaagaac tgcggtggac tgcttcctgc
     3961 gttgggcagc gcaaccggag gacaatctca caaaagaaaa attttacccc aatatagagc
     4021 gagccgagtt gattacaaag ggttatgtgg cctcacaatc cagccatagc cgtggaagcg
     4081 caattgatct tacgctctac cacctggata caggggaact tgtttcaatg ggaagtaact
     4141 tcgattttat ggacgaacgg tcgcatcata cagcaaaagg gataggggat gcagaggcac
     4201 aaaatcgaag atgcttgcgt aaaatcatgg aaagcagcgg atttcagtct tatcgctttg
     4261 aatggtggca ctataagttg attgatgagc cataccccga tacctatttt aattttgccg
     4321 tttcataatg aaagtattgg ttttgtatca gattttggat ttgataaaat ctatgctggt
     4381 catggacaat cttttactgg aaagtggtta aagcatcttt tgtaatacat tgcaaattat
     4441 agtattcaat ctgcccagcg gcagaaaaga aaaaaaccgc tgttgggcag acctttccca
     4501 cgcccatttt gtattttgct ggcggcggtt ttgagaaatc aaggccgccg ttcttttctg
     4561 gcagcaaaag gtgtggcggc ccataggagg atgccgctat gctgttttgc tattgccatg
     4621 ctctaatcca cttccaccac tcaaaaaaag ccttgcccct ccatgaactg aaccccaaaa
     4681 gttgaactat ttaatggact gt
//
LOCUS       DADVXU010000296         2771 bp    DNA     linear   BCT 15-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate 301497-19
            SAMEA8426449-rid11941473.guided.184, whole genome shotgun sequence.
ACCESSION   DADVXU010000296 DADVXU010000000
VERSION     DADVXU010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8426449
            Sequence Read Archive: ERR5555414
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-APR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001005863.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001005863.1
            Genome Coverage       :: 246x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/15/2021 08:28:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,145
            CDSs (total)                      :: 3,083
            Genes (coding)                    :: 3,013
            CDSs (with protein)               :: 3,013
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 27 of 70
            Pseudo Genes (incomplete)         :: 33 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8426449-rid11941473.guided.184"
                     /isolate="301497-19"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019-03"
     gene            110..193
                     /locus_tag="KB953_003141"
     CDS             110..193
                     /locus_tag="KB953_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HBB6868019.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            318..1055
                     /gene="erm(B)"
                     /locus_tag="KB953_003142"
     CDS             318..1055
                     /gene="erm(B)"
                     /locus_tag="KB953_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBB6868020.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1318..2181)
                     /locus_tag="KB953_003143"
     CDS             complement(1318..2181)
                     /locus_tag="KB953_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HBB6868021.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2183..2455)
                     /locus_tag="KB953_003144"
     CDS             complement(2183..2455)
                     /locus_tag="KB953_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBB6868022.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2472..2681)
                     /locus_tag="KB953_003145"
     CDS             complement(2472..2681)
                     /locus_tag="KB953_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HBB6868023.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
BASE COUNT          851 a          487 c          443 g          990 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccaaa attttgtata ataggaattg
       61 aagttaaatt agatgctaaa aatttgtaat taagaaggag ggattcgtca tgttggtatt
      121 ccaaatgcgt aatgtagata aaacatctac cgttttgaaa cagactaaaa acagtgatta
      181 cgcagataaa taaatacgtt agattaattc ctaccagtga ctaatcttat gactttttaa
      241 acagataact aaaattacaa acaaatcgtt taacttctgt atttatttat agatgtaatc
      301 acttcaggag tgattacatg aacaaaaata taaaatattc tcaaaacttt ttaacgagtg
      361 aaaaagtact caaccaaata ataaaacaat tgaatttaaa agaaaccgat accgtttacg
      421 aaattggaac aggtaaaggg catttaacga cgaaactggc taaaataagt aaacaggtaa
      481 cgtctattga attagacagt catctattca acttatcgtc agaaaaatta aaactgaaca
      541 ctcgtgtcac tttaattcac caagatattc tacagtttca attccctaac aaacagaggt
      601 ataaaattgt tgggaatatt ccttaccatt taagcacaca aattattaaa aaagtggttt
      661 ttgaaagcca tgcgtctgac atctatctga ttgttgaaga aggattctac aagcgtacct
      721 tggatattca ccgaacacta gggttgctct tgcacactca agtctcgatt cagcaattgc
      781 ttaagctgcc agcggaatgc tttcatccta aaccaaaagt aaacagtgtc ttaataaaac
      841 ttacccgcca taccacagat gttccagata aatattggaa gctatatacg tactttgttt
      901 caaaatgggt caatcgagaa tatcgtcaac tgtttactaa aaatcagttt catcaagcaa
      961 tgaaacacgc caaagtaaac aatttaagta ccgttactta tgagcaagta ttgtctattt
     1021 ttaatagtta tctattattt aacgggagga aataattcta tgagtcgctt ttgtaaattt
     1081 ggaaagttac acgttactaa agggaatgta gataaattat taggtatagg gcacctctaa
     1141 taactaccaa ttaattcacc tcaaaatgaa attagaaaaa aacagcgaaa tttttaaatc
     1201 tatttcttat cgatacaaat tcctcgtagg cgctcgggac cccaacatga ggaaatgttt
     1261 ttataaaaaa agagtagtta ccgttttttg gtaactactc ttttcctttt ttaaggttta
     1321 aatacctgga agtttaggtg ttttgggtat tggtggtgta ggtagctgca agctttccat
     1381 tttttgttga attgctttaa attcaggggt ttcttggtgt tgattttgaa ccattttttg
     1441 ctctattctc tccaaagtcg gttgaatttc tttccctgat actttacgat ttaattctct
     1501 ttctaaggtc tctttcggac taatggaagg cgtttctaag cttgaataga gtttcactcc
     1561 ttctctattg taaagcctta tatcgctaaa taagcccgtt ttatgaaggg tttctaaatt
     1621 ggtcggtaag tttttgacaa caatatcatg cgcttgtttt ggtgttgccc tagctgtcat
     1681 tggatcatct gcatacatgg tttcatatcg ttcaatcgtt cctaagtatg attcaatttt
     1741 cggtactgcc atgacgtata tttttgtttc ataaccttta gattgaagca ttgtggctgt
     1801 ttgaatggga acgtctgttg ttcgtcctgt tccttcaatc actaaattat agccttgatc
     1861 gcttaaacgg ctaataagtg cttctgtcat gcgattcgaa taaggggtaa cgtgttttac
     1921 tacgtctttt tcatagagtt tcactaattc gtcaaaattt gggtgctgtt gtttaaaggt
     1981 atcattatca atgacaataa cattcccttg tgtttcttca aaaattgctg atcgcaaact
     2041 ggttttccct gaccctggtt gcccaccaag taaaaaagcg gttggcgatt caaccgctag
     2101 tctattttta gttagtcgtt caacattctt ttctaaacga tcttcaaatt gcttttcggt
     2161 gaagtcagta atattcgcca tgttaagcca ctttctcttt attcaaaatt tgttttgaat
     2221 attgattcat tgaccgccaa tactcatgaa tagcttgtaa ttcttcaaat ggttgttgaa
     2281 ataaatcaac ttcttgtgcc acttctaatt gatttaaaag attcacttct agtaaacgag
     2341 tatttttagt atctagttca tgattcaaaa cataatttaa tacccgatta taaacacttg
     2401 ccgataactc attaatgatt tcaatttcaa atgttttttc atacgtaact gccatttcat
     2461 tttcactcct tttaaagttt gtcaggtaaa tatttccgta tatattcttc taaggcttga
     2521 tccataactt cttttacgtt tccgccattc tttgctgttt cgatttttat gatatggtgc
     2581 aagtcggcac gaacacgaac cgtcttatcc cccattatat ctttttttgc gctgattggc
     2641 gtatcatttc gttttgcttt ctgtgcgcct aaatttccca cgatcgctca cttctttcta
     2701 tttcttctta ctatttattt tatcatcacc tatcacaaat cacaagtgat ttgtgattaa
     2761 tcacttgtga t
//
